LOCUS       DAOOKS010000001        11112 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_6611_length_11112_cov_3.953875, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000001 DAOOKS010000000
VERSION     DAOOKS010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11112
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6611_length_11112_cov_3.953875"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1030
                     /locus_tag="PK112_00005"
     CDS             <1..1030
                     /locus_tag="PK112_00005"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HPC10463.1"
                     /translation="LTAVVLKAIHRDPAKRYQTALEMKEALVSASGVTLPFRYATPTP
                     PPPPPRKPARPGVKWAAAGVLVAAAVAAYLFFGRGQPSGPDTTAALEPPPVPVPAAPP
                     DGGRLEAGAAVRLVWSSAGDSTVHYRVQIADNADFLNSATYENLSDTFRILDTSFAAE
                     VMYWRVRAVSREGLASAYSAPGRFAVAAGPAATSGTLKVTANRPVRLKLDNAVVAQRV
                     TSWDTVLAAGDYRLEANHPGAAPSTQQQPVRVVGGRTARVSFEFALPADSGVLFVGSD
                     PVGNEIYLNGRPRGSTPQFFKLPVGEYRVQVKKGDVASSPEVGRIVQHGDTAWFMYDF
                     TGDSVAVE"
     gene            1125..2168
                     /locus_tag="PK112_00010"
     CDS             1125..2168
                     /locus_tag="PK112_00010"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HPC10464.1"
                     /translation="MSRFRMRLVAALLLSAAALPAVSLVAQTPADVPGELEVVRQHYA
                     RGDWQQCLDRLDKLLARPALTAYDSIAVFEMQSLVYRMMGEDYLERSYTCLQKIAAIS
                     PCLVRLPRDVWPQDLKNRWYSVVSPLGALTCKSTGPTEPQTVAVWYFDNFSVQKYLDE
                     LGSLGSALGLMFQEEFKKLGSLQVVEREKFDYIINEQKLAAGGAVDQATAIQAGKILQ
                     AHVMVFGSFTQLDRSHTMVQARAVNVETSEIIASVSAEGNPDKYFQIVKDLVQELCQA
                     LDVKLSKDDMKLIEEGGTGSYDATVAFAKGVEYEKKHDYKLAYEHFKKAFELDPGFKE
                     AETKMNVYVHLAA"
     gene            2221..3531
                     /locus_tag="PK112_00015"
     CDS             2221..3531
                     /locus_tag="PK112_00015"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HPC10465.1"
                     /translation="MRMRMGLALLGALLMVSCAPNLVTQGRKLTDQARYEQARECLFK
                     ELSVNPTNAAAWREVGIAYYKEGRAAEADVAFKNAVNQPAPDPAAYVYLGLIQENKGY
                     PDSAVGYYIAALDRYPTGRSAATVRKRLDQLLQEELRNRLPQVVAGEGQLDASAIPEN
                     SVAVLNFGGARLDPEVAPVARGLSALVAADLAKVSSIRSIERLKVETLLAEQRLRDMG
                     VADSLSAVQKGYLLGAHTVVTGELMSIGDKIRLDAALVDVVRATPTPSAPTEDALDQF
                     YRFQKRFVFSLLDSLHVTLTPEERARLDDVPTESSLAFLAYCRGLQYQGEGYYKAAQV
                     EFQQALKHDGSFSQARTELDNTNTLMSLQNMGDVSAGQVESAVGQDFLGGAPDDLGAS
                     LQGLHDDLGIVPADGEQDGTSDSEEPPVPAGVGAAQVRGNLDGK"
     gene            3521..4642
                     /locus_tag="PK112_00020"
     CDS             3521..4642
                     /locus_tag="PK112_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10466.1"
                     /translation="MGNSHALRRWAALLLAVAGLASAAECQVIYGQPASGRASFVYTS
                     WDLDSTGAGTTSIAQSLLIVSGFLPLRDNLEARFFLPTAAGDLESPEAAADVSGLGDL
                     RLQVSRSLSNDQILLSIGLSLPTGKKELRFADERMIVQVLSENFLDFPVRRFGEGLGL
                     NALAGFARNYGRATVAGGVSYSYTGAYSPYEGIDDYNPGDVLSANLGADLKAGRAVWS
                     AAATLSYRTTDTREGAKMFRQSTDLDFRLANQTQLDRLSLLLLTRYLLRGESALYDQY
                     EESLNRRLYGNEFHLSGRARYAVSEGWGLGPAAGLRLIAADDEFGASTVVRLGVEASR
                     KIAAHGSLDVDLRYLTGSADGGAIDLSGWQASVSLQAAM"
     gene            4666..5571
                     /locus_tag="PK112_00025"
     CDS             4666..5571
                     /locus_tag="PK112_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10467.1"
                     /translation="MNRIWTSTGAALLAGAVLAAFAGGCADTVTGRQPGRTVTVAVQL
                     QKNTSALQESFQAFILTVARVRDGVLLAADTSYDLTASVVTVTVENLPSGEPLEFVVS
                     ILNDAGVEVYGGRQVQEIAPDLTTVIDLPLTPSVPMLKFVPRYIAPLDSGAVLDTVIQ
                     ACFYRQPALYGIAFRVYYRNDLVRIDSVGPSPALAALVAGGEVLFLPPQFVQVQTGLG
                     YWAVALTQTVPGRALVDAAARGPLVDVHVQSLGVGAPAAASTSIWLAPTSMTLTDGTA
                     IDSTQVTAVLYTDDCYIDAGSPGPM"
     gene            complement(5654..9205)
                     /locus_tag="PK112_00030"
     CDS             complement(5654..9205)
                     /locus_tag="PK112_00030"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPC10468.1"
                     /translation="MDADAARRSSYSGPLDVEGFVPTIDPATGAPVFRAALPLAPADY
                     PDDIYWDNRISPSVPGVSGTVRAVAVYNGQLIVGGSFEVAGGVLAHNIASWDGTSWSA
                     LGSGANDWVRSLTVYDGSLIAGGDFDTAGGIQANRIAAWNGLSWSALGAGMDRSVKAL
                     AAYDGRLVAGGNFDTAGAVAARRIAAWNGSSWSPLGSGMNGNVEAVTVWDHRLIAGGW
                     FTTAGGISAARVAAWDGSSWAPLASGIADGGVYALVEYNGNLIAGGYFTTAGGTGANH
                     IASWDGRTWSPLGTGMNGSVYSVGVYDGHLVAGGWYATAGGTAAENIAAWNGSAWSAL
                     GSGAGSGVYAVTPYGSALAAGGAFGVAGDIRAVGIAAWNGAVWAALGAGTDDLVYALT
                     LYNGRVIAGGRFTSIGGVQANRIAAWDGSSWSPLGVGLNDDVYALTVYDGRLTAGGRF
                     TASGEQGVSRVAAWDGSAWSPVGPGVNGDGVYALTEYNGDLIAGGWFDTAGWVVADNI
                     AAWDGSSWRPLGSGMADGDVYALVEYNGDLIAGGWFYSAGDTTAPYVAAWNGSTWSPL
                     VSPDEEFNPVNALTVYDGRLIAALDVGAYKSDAQTAYNGIAAWDGTAWSWLGTGIADD
                     NASVDAVAVYLGRLIAGGRFITADDPAAYFIASWDGRAWSAMGSGLSGSAASFTVAND
                     RLFVGGRFTIAGGKVAAFLAEWTKGVDTDSDGIDDDLDNCPTTYNPDQADRDVDGLGD
                     ACDTGPELALSPETVDIGWELTRGEFVVKNAGYGTLEFRLDTLPEWCTARPVSGSLGA
                     GGEAVIEVEVDRRGTLMGDHAGVVAISSNGGAGGVLVRMYTPIAPLVGSDSVGIEVGE
                     EIRIPFNGLAEAGSLLSRLQVVSDAGELAYGVAVDTEYVGLRIPVTVVYIYPDEGWSF
                     PELAGIRVMFADSIVDAHGVPGPQGYAFTYVNGAVVWPGDTDGDGVVDERDILPIGLY
                     YGSGGPGRGEGQGTEWGPYSVAVLAEGVVWRPLRAVYADADGSGEVDALDVCAITENW
                     RARADGWKRGVGEGAGVEGLPKELSPGVAAALRAALAECPEGAGKAALAEVLGGIGEP
                     VLPGEVTLYQNYPNPFNPSTVVEFYLPAAGEIRVGVWNVLGQEVAELYRGWHASGYGR
                     LEWSGRTRGGGEAAAGLYFYRLTAGDRTLTRRMLLLK"
     gene            complement(9543..>11112)
                     /locus_tag="PK112_00035"
     CDS             complement(9543..>11112)
                     /locus_tag="PK112_00035"
                     /inference="COORDINATES: protein motif:HMM:NF040218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPC10469.1"
                     /translation="EPLQCAAAANPARGPAPLTVNFSASASGGEPPYTFAWSFGDGGS
                     SSAPNPAHAYSAVGDYAASLAVTDSRAIACGDTLLIQVLGPELALSPETVDIGWELTR
                     GEFVVKNAGYGTLEFRLDTLPEWCTARPVSGSLGAGGEAVIEVEVDRRGTLMGDHAGV
                     VAISSNGGAGGVLVRMYTPIAPLVGSDSVGIEVGEEIRIPFNGLAEAGSLLSRLQVVS
                     DAGELAYGVAVDTEYVGLRIPVTVVYIYPDEGWSFPELAGIRVMFADSIVDAHGVPGP
                     QGYAFTYVNGAVVWPGDTDGDGVVDERDILPIGLYYGSGGPGRGEGQGTEWGPYSVAV
                     LAEGVVWRPLRAVYADADGSGEVDALDVCAITENWRARADGWKRGVGEGAGVEGLPKE
                     LSPGVAAALRAALAECPEGAGKAALAEVLGGIGEPVLPGEVTLYQNYPNPFNPSTVVE
                     FYLPAAGEIRVGVWNVLGQEVAELYRGWHASGYGRLEWSGRTRGGGEAAAGLYFYRLT
                     AGDRTLTRRMLLLK"
BASE COUNT         1897 a         4150 c         3367 g         1698 t
ORIGIN      
        1 gctgaccgcg gtcgtcctga aagccatcca ccgggatccg gccaagcgat accagacggc
       61 cctcgagatg aaagaggcgc tagtgtccgc gtcgggtgtg acgctgccgt ttcgctatgc
      121 gacgccgacg ccgccgcccc cgccgccgcg gaagccggcg cgcccggggg tgaagtgggc
      181 cgcggcgggc gtgctggtcg cggccgcagt cgccgcctac cttttcttcg gacgcggcca
      241 accgtccgga ccggatacga cagccgccct ggaacccccg ccggttccgg tgccggcggc
      301 gccgccggac ggcgggcggc tggaggcggg cgcggcggtt cggctggtgt ggagctcagc
      361 cggcgattca accgtgcact accgcgttca gattgccgac aatgcggact ttctgaattc
      421 ggccacgtac gaaaacctca gcgacacgtt tcggatcctc gacacgagtt tcgcggcgga
      481 ggtgatgtac tggcgggtgc gggcggtcag ccgggagggt ctggccagtg cctactcggc
      541 gccgggcagg ttcgcggtgg cggccgggcc ggcggcaacc agcggcacac tgaaggtcac
      601 ggcaaaccgg ccggttcgct tgaagctcga taacgccgtt gttgcgcagc gcgttacctc
      661 ctgggacacg gtgctcgcgg ccggcgacta ccggctcgaa gcgaatcatc cgggagcggc
      721 gccgagcacg cagcaacagc cggtccgcgt ggtcggcggg cgcacggccc gggtctcgtt
      781 cgaattcgcg ctccccgccg attcgggcgt gctgtttgtc gggtccgatc ccgtggggaa
      841 cgagatctac ctcaacggcc ggccgcgggg atccaccccc cagttcttca agctgccggt
      901 gggcgaatac cgcgttcagg tgaagaaagg ggacgtcgcg tcctcgcccg aggtggggcg
      961 catcgttcag cacggcgaca cggcgtggtt catgtatgat ttcaccggcg actcggtggc
     1021 ggtggagtga gccggccaaa aaaggtcttt actgcggtcc ggggatgccg tatcatgaag
     1081 cgggttcaaa aaggaaaatc gcaaaattcc acaaggagat tccgatgagt cggtttcgca
     1141 tgcgcctcgt ggcagctctc cttttgtcgg ccgccgcgct cccggcggtg tcacttgtgg
     1201 cccagacacc ggccgatgtc cccggcgagc tggaggtcgt gcgccagcac tatgcccgcg
     1261 gcgactggca gcaatgcctc gaccgcctgg acaagctgct ggcccggccc gccctcacgg
     1321 cctatgacag catcgccgtg ttcgaaatgc agagcctcgt gtaccgcatg atgggggagg
     1381 actacctcga gcggtcgtac acctgcctgc agaagatcgc cgccatcagc ccctgcctgg
     1441 tgcggctccc ccgcgatgtc tggccgcagg atctgaagaa ccggtggtac tcggtggtga
     1501 gtccgctcgg cgcgctcacc tgcaagtcga cgggcccgac cgaaccgcag accgtcgccg
     1561 tctggtactt cgacaacttc tcggtccaga agtacctcga tgaactcggg tcgctcggtt
     1621 cagcgctcgg cctgatgttt caggaggagt tcaagaagct gggcagcctg caggtggtgg
     1681 agcgcgagaa attcgactac atcattaatg agcagaagct ggcggccggc ggcgccgtcg
     1741 atcaggcgac cgcgatccag gccggcaaga tcctgcaggc ccacgtgatg gtgttcggct
     1801 ccttcacgca actggaccgc agccacacga tggtccaggc ccgcgccgtc aacgtcgaga
     1861 cctcggaaat catcgcctcg gtgtcggccg aggggaaccc cgacaagtac ttccagatcg
     1921 tcaaggacct cgtgcaggaa ctgtgccagg cgctcgatgt caagctgtcg aaggacgaca
     1981 tgaagctgat cgaggagggc ggcaccggat cgtacgacgc cacggtcgct ttcgccaagg
     2041 gcgtcgagta cgagaagaag cacgactaca agctggccta cgagcatttc aagaaggcct
     2101 ttgaactcga ccccgggttc aaggaggccg agaccaagat gaatgtctac gtccatctgg
     2161 ccgcgtagcc gcgccgtccg gatgaacccg tctttcgcaa ccgaagtgag gcaagacact
     2221 atgcgcatgc gaatgggcct cgcgctcctc ggcgccctgc tgatggtttc gtgcgctccg
     2281 aacctggtca cccagggccg gaagctgacg gatcaggccc gctacgagca ggcccgcgaa
     2341 tgtctcttca aggagctgtc ggtcaatccg accaatgcgg cggcctggcg ggaggtgggg
     2401 atcgcgtact acaaggaggg ccgcgcggcg gaagcggacg tggccttcaa gaacgccgtc
     2461 aaccagccgg cccccgatcc cgcggcctac gtctacctcg gcctgattca ggagaacaag
     2521 ggctatcccg attcggccgt cggctactac atcgccgccc tcgaccggta cccgaccggc
     2581 cgctcggccg cgaccgtccg caagcgtctc gatcagctcc tgcaggagga gctgcggaac
     2641 cggcttcccc aggtggtggc cggggagggc caattggatg cgagcgcgat tccggagaac
     2701 tcggtggcgg tcctgaattt cggcggcgcc cgcctcgacc ccgaggtcgc cccggtggcg
     2761 cgcgggctgt cggcgctggt cgcggccgat ctcgccaagg tctcctcgat ccgctcgatc
     2821 gaacggctga aggtcgagac gctgctggcc gagcagcggc tccgcgacat gggtgtcgcc
     2881 gactcgctga gcgcggtgca gaagggctac ctgctggggg cccacaccgt cgtcaccggc
     2941 gaactcatga gcatcggcga caagatccgc ctcgacgcgg cgctggtgga tgtcgtgcgg
     3001 gccacgccga ctccgtccgc cccgaccgag gatgccctcg accagttcta ccgcttccag
     3061 aaaagattcg tcttctctct gctggactcg ctccacgtca cgctgacgcc cgaggagcgg
     3121 gcccggctgg atgatgtgcc gaccgaatca tcgctggcct tcctcgccta ctgccggggc
     3181 ctgcagtacc agggcgaagg gtactacaag gcggcgcagg tggagttcca gcaggcgctc
     3241 aagcacgacg gttccttttc ccaggcccgg acggaactgg acaacacgaa caccctgatg
     3301 agcctgcaga acatggggga cgtgagcgcc ggccaggtgg agtccgcggt cgggcaggat
     3361 ttcctcggcg gcgcccccga cgatctcggg gccagcctgc aggggctcca cgacgatctc
     3421 gggatcgttc cggccgacgg cgaacaggac gggacgtcgg attcggagga gccgccggtg
     3481 ccggccgggg tcggcgcggc ccaggtaagg gggaacctcg atgggaaata gtcatgcact
     3541 gcggcgctgg gccgcgctgc tgctggcggt ggcgggcctg gcctcggcgg ccgagtgcca
     3601 ggtgatctac ggccagcccg cctccgggcg cgcttcgttc gtgtacacga gctgggatct
     3661 cgattcgacc ggggccggca cgaccagcat cgcgcagagc ctcctgatag tgagcggttt
     3721 cctcccgctg cgggacaacc tggaggcccg cttcttcctc cccaccgcgg ccggggatct
     3781 cgaatcgccg gaggccgcgg ccgacgtctc cggcctcggc gaccttcgcc tgcaggtgag
     3841 ccggtcgctg agcaacgacc aaatcctgct aagcatcggc ctcagcctcc ccaccggcaa
     3901 gaaggagctg cgctttgccg atgagcggat gatcgtgcag gtgctgtccg agaatttcct
     3961 ggacttcccg gtgcggcggt tcggcgaggg tctcgggctg aacgccctgg cggggttcgc
     4021 ccgcaactac ggccgcgcca cggtggccgg cggcgtctcc tactcctaca ccggcgccta
     4081 ctcgccgtac gaggggatcg acgattacaa cccgggcgac gtgctgtccg ccaacctcgg
     4141 ggccgacctc aaggccggcc gcgcggtctg gtcggcggcg gccacgctca gctaccgcac
     4201 gaccgacacg cgcgagggcg ccaagatgtt ccgccagagc accgacctcg atttccgcct
     4261 ggcgaaccag acgcaactgg accggctcag ccttctcctg ttgacccggt accttctgag
     4321 gggagagagc gcgctctacg accagtacga ggaatcgctg aaccgccggc tctacggcaa
     4381 cgagttccat ctctccggcc gcgcccggta tgccgtatcg gaggggtggg gtctcgggcc
     4441 ggcggccggc ctccgcttga tcgcggccga tgacgaattc ggcgcgagca cggtggtccg
     4501 gctgggcgtg gaggcaagca ggaaaatcgc cgctcacggc agcctcgatg tggacctccg
     4561 gtacctcacc gggagcgccg acggcggggc catcgatctg agcggctggc aggcctcggt
     4621 gagcctccag gccgcgatgt gaagggggat ttcgtgggaa gaactatgaa caggatatgg
     4681 accagtaccg gggcggctct tctggccggc gccgtcctgg cggctttcgc cggcggctgc
     4741 gccgacaccg tcaccggccg gcagcccggc cgcaccgtca ccgtggctgt ccaactgcag
     4801 aagaatacct cggccctcca ggagtcgttt caggctttca tcctcacagt cgcgcgggtc
     4861 agggacggcg tgcttctggc ggccgacacc agctacgacc tgaccgcctc ggtggtcacg
     4921 gtcacagtcg agaacctccc ctccggggag ccgctggaat ttgtcgtgag catcctcaac
     4981 gacgccggcg tggaggtgta cggcgggcgc caggtgcagg agatcgcacc cgacctgacg
     5041 accgtcatcg accttccgct cacgccgtcg gtgccgatgc tcaagttcgt gccgcgctac
     5101 atcgctcctc tcgactccgg ggcggtgctc gatacggtca tccaggcctg cttctaccgg
     5161 cagccggcgc tgtacgggat cgcttttcgc gtgtactatc ggaacgacct ggtgcgcatc
     5221 gacagcgtcg gcccgtcccc cgctctggcg gccctggtgg ccggcggcga agttctgttc
     5281 ttgcccccgc aatttgtgca ggtgcagacc ggtctgggct actgggcggt ggcgctcacg
     5341 cagactgtgc cggggagagc gctcgtcgat gccgccgcca gggggccgct cgtcgatgtg
     5401 cacgtccaat cgcttggcgt cggcgccccc gcggccgcct cgacatccat ctggctggct
     5461 ccgacctcga tgacgctcac cgacggcacg gcgatcgaca gcacgcaggt gaccgcggtt
     5521 ctctacacgg atgactgcta catcgatgcc ggctcccccg gtccgatgta gcgagagggc
     5581 ggcggttgcc gccggccccg cacgtgacag tcgaaagttg cgcgaccgcc ggcgtcttgg
     5641 gcgccggcgc gtgctacttt agcagcagca tgcggcgggt gagcgttcgg tcgcccgccg
     5701 tcagccggta aaagtacagg cccgccgccg cctccccgcc accccgtgtc cgccccgacc
     5761 actccagccg cccatacccc gacgcgtgcc acccccggta caactccgcc acctcctgac
     5821 ccaggacgtt ccacactccc acccggatct cccccgccgc cggaagataa aactccacca
     5881 ccgtcgacgg attaaacgga ttcggatagt tctggtacaa cgtcacctcc cccggaagca
     5941 ccggctcccc gatccccccc agcacctccg ccagcgccgc cttccccgcg ccctccggac
     6001 actccgccag cgccgcccgc agcgccgccg ccacccccgg actcaactcc ttcggcaacc
     6061 cctccacccc ggccccctct cccacacccc gcttccaccc gtccgccctc gcccgccagt
     6121 tctccgtgat cgcgcacaca tccagcgcgt ccacctcccc cgatccgtct gcatccgcgt
     6181 acaccgcccg cagcggacgc cacaccaccc cctccgccaa caccgccacc gagtacggac
     6241 cccactccgt cccctggccc tccccccggc ccggcccccc cgagccgtag tacaacccga
     6301 tcggcaaaat gtcccgctcg tccaccaccc cgtccccatc cgtgtccccc ggccacacca
     6361 ccgcaccgtt cacatacgtg aacgcgtacc cctgaggccc cggaactccg tgcgcatcaa
     6421 caatcgaatc cgcaaacatc acccgaatcc ccgccagctc cggaaacgac caaccctcgt
     6481 ccgggtagat gtacaccacc gtgaccggaa tccgcagccc cacgtactcc gtatccaccg
     6541 ccacaccata cgccagttcc cccgcgtccg acaccacctg cagccgcgac aacagcgacc
     6601 ccgcctccgc caggccgttg aacgggatcc gtatctcctc ccccacctcg atccccacgc
     6661 tgtcagaccc caccagcggc gcgatcggcg tgtacatacg caccaacacc ccgcccgcgc
     6721 ccccgttgct gctgatcgcc accacgcccg catggtcacc catcagcgtc ccccgacggt
     6781 ccacctccac ctcgatcacc gcctcccccc ccgctcccag cgaacccgac accggcctcg
     6841 ccgtgcacca ctccggcagc gtgtcgagcc ggaactccag cgtcccgtac cccgcgttct
     6901 tcaccacgaa ctccccgcgc gtcaactccc aaccaatatc caccgtctcc gggctcaacg
     6961 ccagctccgg acccgtatcg cacgcatccc cgaggccatc gacatcgcga tcggcctggt
     7021 cggggttgta ggtggtcggg cagttgtcga gatcatcgtc gatgccgtcg ctatcggtat
     7081 ccacgccttt ggtccattcg gcaaggaaag ctgcgacctt tcctcccgct atcgtgaagc
     7141 gacccccgac aaagaggcgg tcgttggcga cggtgaagga cgctgccgag ccgctgagcc
     7201 ctgatcccat ggccgaccag gcccggccat cccacgaggc aatgaagtag gccgccggat
     7261 cgtccgccgt gatgaagcgc ccgccggcga tcaaacgccc gagatatacg gccacggcat
     7321 cgactgaggc gttgtcatcg gcgatgcccg ttcccagcca cgaccaggcc gtgccgtccc
     7381 acgcggcgat cccattgtag gcggtctgtg cgtcactctt ataggccccc acatccagag
     7441 cggcgatcag tcttccgtca tagaccgtca gagcgttgac ggggttgaat tcctcgtctg
     7501 gcgacacgag cggcgaccac gtggaaccgt tccacgcggc gacatacggg gcagtagtat
     7561 cccctgccga gtaaaaccat ccaccggcga tgaggtcgcc gttgtattcg accagtgcgt
     7621 agacgtctcc gtccgccatc cccgatccca gcgggcgcca tgaggagccg tcccaggcgg
     7681 cgatgttgtc ggcgacgacc caccctgccg tatcgaacca tccgcccgca atcaagtcac
     7741 cgttgtactc tgtcagggcg tacacgccgt cgccgttcac gccgggtccg acaggggacc
     7801 aggcggaacc atcccaagcg gccacgcggc tcacaccctg ctcgccggaa gccgtgaagc
     7861 gacccccggc agtcaggcgg ccgtcgtaca cagtcagggc gtagacatca tcgttcaacc
     7921 cgactcctaa cggagaccac gaggaaccgt cccaggcggc tatgcggttg gcctgaaccc
     7981 cgccgatcga agtgaatcgt ccaccggcga tgacgcgccc gttgtagagg gtgagggcgt
     8041 agacaaggtc atccgtcccg gcccctaagg ccgcccacac ggcgccgttc caggcggcga
     8101 tcccgaccgc ccgtatgtcg cccgcaaccc cgaaagctcc ccctgccgcc agcgcagagc
     8161 cgtagggagt cacagcatag accccgctgc cggctccgga tcccagtgcg gaccaggcgg
     8221 aaccgttcca ggcggcgatg ttctcggcgg cggtccctcc ggccgttgcg taccatccgc
     8281 ccgcaacgag atggccgtca tagacgccca ccgaatacac agaaccgttc atccccgttc
     8341 cgagcggact ccatgtccgg ccgtcccagg aggcgatatg gttggcgccg gttccgcccg
     8401 cagtggtgaa atatcccccc gcgatcaggt tgccgttgta ttcgaccagc gcgtagacgc
     8461 ctccatcggc gatcccggac gccagcggcg cccaggagga accgtcccag gcggcgactc
     8521 gggcggcgct gatgccgccc gctgtcgtga accatccccc ggcaatcaac cggtgatccc
     8581 agacagtcac ggcttccacg ttcccgttca tgccggatcc aagaggggac caggatgagc
     8641 cattccacgc ggcaatgcgg cgcgcggcaa ccgcgccggc ggtgtcaaag ttgccgcccg
     8701 cgaccaggcg accgtcgtag gccgccaacg ccttcaccga gcgatccatc cccgctccga
     8761 gggccgacca cgacaagccg ttccacgcgg cgatgcgatt cgcttgtatt ccgccggccg
     8821 tgtcaaaatc tccgccggcg atcagagaac cgtcatacac cgtaagtgag cggacccaat
     8881 cgttggctcc ggatccaaga gcggaccagg aggtgccatc ccaggacgca atattgtggg
     8941 ccaatacacc gccggctacc tcaaagcttc cgcccacgat gagttgcccg ttgtacacgg
     9001 caactgcccg cactgtaccg gaaacgccgg gcacggaggg agagatgcgg ttgtcccagt
     9061 agatgtcgtc ggggtagtcg gccggcgcga gcggcaaagc cgcgcgaaag accggtgcgc
     9121 cggtcgcggg atcgatggtc gggacgaatc cttcaacgtc gagcggcccc gagtaggagg
     9181 agcgccgggc cgcatccgcg tccaaccgac cgtcgggagt gagatacgtt tcgatccttg
     9241 ggctgtaccg ggtatcgcgg gtgggacagc ccgcagacat gcgtctggaa agcgcggagg
     9301 gccggtctgc cgatgatgcg gcgagaccgc ccgccagcaa gagcccgagg actgcgctca
     9361 tacctatgcg actcatgcgt aacctccttg cgtgcgaacc cccgcgcgac ctccggcgaa
     9421 gcctacagga gagccttcct gcgggctgcc ggtcgattca tggtgtcggc gggcaatata
     9481 ggccgcccga caccgggcga caacgagatt ctgcggatcg gcgtcttggg cgccggcgcg
     9541 tgctacttta gcagcagcat gcggcgggtg agcgttcggt cgcccgccgt cagccggtaa
     9601 aagtacaggc ccgccgccgc ctccccgcca ccccgtgtcc gccccgacca ctccagccgc
     9661 ccataccccg acgcgtgcca cccccggtac aactccgcca cctcctgacc caggacgttc
     9721 cacactccca cccggatctc ccccgccgcc ggaagataaa actccaccac cgtcgacgga
     9781 ttaaacggat tcggatagtt ctggtacaac gtcacctccc ccggaagcac cggctccccg
     9841 atccccccca gcacctccgc cagcgccgcc ttccccgcgc cctccggaca ctccgccagc
     9901 gccgcccgca gcgccgccgc cacccccgga ctcaactcct tcggcaaccc ctccaccccg
     9961 gccccctctc ccacaccccg cttccacccg tccgccctcg cccgccagtt ctccgtgatc
    10021 gcgcacacat ccagcgcgtc cacctccccc gatccgtctg catccgcgta caccgcccgc
    10081 agcggacgcc acaccacccc ctccgccaac accgccaccg agtacggacc ccactccgtc
    10141 ccctggccct ccccccggcc cggccccccc gagccgtagt acaacccgat cggcaaaatg
    10201 tcccgctcgt ccaccacccc gtccccatcc gtgtcccccg gccacaccac cgcaccgttc
    10261 acatacgtga acgcgtaccc ctgaggcccc ggaactccgt gcgcatcaac aatcgaatcc
    10321 gcaaacatca cccgaatccc cgccagctcc ggaaacgacc aaccctcgtc cgggtagatg
    10381 tacaccaccg tgaccggaat ccgcagcccc acgtactccg tatccaccgc cacaccatac
    10441 gccagttccc ccgcgtccga caccacctgc agccgcgaca acagcgaccc cgcctccgcc
    10501 aggccgttga acgggatccg tatctcctcc cccacctcga tccccacgct gtcagacccc
    10561 accagcggcg cgatcggcgt gtacatacgc accaacaccc cgcccgcgcc cccgttgctg
    10621 ctgatcgcca ccacgcccgc atggtcaccc atcagcgtcc cccgacggtc cacctccacc
    10681 tcgatcaccg cctccccccc cgctcccagc gaacccgaca ccggcctcgc cgtgcaccac
    10741 tccggcagcg tgtcgagccg gaactccagc gtcccgtacc ccgcgttctt caccacgaac
    10801 tccccgcgcg tcaactccca accaatatcc accgtctccg ggctcaacgc cagctccgga
    10861 cccagcacct ggatgaggag agtgtcgccg caggcgatgg cccgggagtc ggtgacagcg
    10921 agcgaggccg catagtcgcc caccgcgcta taggcgtggg ccgggtttgg cgcggaactg
    10981 ctgccgccgt cgccgaagct ccacgcgaac gtgtagggag gttctccgcc agaggcgctc
    11041 gcgctgaaat tgaccgtcag cggcgccggg ccgcgggccg gattggccgc cgccgcgcac
    11101 tgcagcggct ca
//
LOCUS       DAOOKS010000002        10954 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_6751_length_10954_cov_4.018626, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000002 DAOOKS010000000
VERSION     DAOOKS010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10954
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6751_length_10954_cov_4.018626"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="PK112_00040"
     CDS             <1..715
                     /locus_tag="PK112_00040"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HPC10470.1"
                     /translation="GPVRQSRVFMTYGGSFENLLTLAHELGHAYHQQVITEKPYFAQQ
                     YPMPLAETASIFSETLVTDAALGQAADAGERLMLLDQKLQSAYVMFTDLHCRYIFDRA
                     FYAERRNGMVPAERLSEMMVTAQKEAFAGMLDDSGCHPLFWASKLHFYMTSAPFYNFP
                     YVFGFLFAGGVYDRAKREGKKFAKDYRALLADTGSMTCEELARTHLGVDLADEGFWRS
                     AVDRALAEVEAYVKLAKSM"
     gene            858..1361
                     /locus_tag="PK112_00045"
     CDS             858..1361
                     /locus_tag="PK112_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013932680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HPC10471.1"
                     /translation="MENDFTVEVLVEIPKGSRNKYEYDKARGLFRLDRMLFSAVHYPS
                     DYGFILNSLGGDRDPLDALVLTWEPTFPGCLIDARPVGLFRMQDEKGPDEKILCVPIH
                     DPMWNRIRALEEVPPHLVKEIEHFFQVYKELEEKKTAVEGWEDRAAAERVVREAQERW
                     RKAGEHD"
     gene            1662..4262
                     /locus_tag="PK112_00050"
     CDS             1662..4262
                     /locus_tag="PK112_00050"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPC10472.1"
                     /translation="MRAVFVLVMLTVLCGFVFTEDFRVGQSCVPPSGDEYVPDEIVVK
                     FKDGVGRQAVSAALADLGATTISTNRQIGFHQLRIPAGTTVEEMVAAFGARPDVEYAE
                     PNYIAHAFMTPNDPYYTYQWHMPLIKMPAAWDQTTGSPSVIVAVVDCGVAYETYGSFT
                     QAPDLAGTSFVPGYDFINNDSHPNDDCAHGTHVAGTVAQTTNNNLGVTGVAFNCSIMP
                     VKVLDASGSGSYTAIANGVTFAADNGAHVINMSLGGSYNSSTLQNAVVYAYNKGVTIV
                     CAAGNAGTSTPQYPAAYSQCISVSAVRYNKTYTSYTSYGSTIDICAPGGDVSVDQNGD
                     GYVDGVLQQTHDGTNYGTFGYYFYEGTSMASPHVAGVAALLIAKNGGSMAPADVRAAL
                     QNTAEDLGAAGWDQYYGYGLVDANAALLSISTNNPPVAAFSGTPTSGTAPLTVVFTDA
                     STNNPTSWSWNFGDGGTSTAQNPTHTYASAGTYTVTLTATNAYGSDSETKTGYITVSA
                     PPTYPPVAAFSGTPTSGTAPLTVVFTDASTNTPTSWSWNFGDGGTSTAQNPTHTYTSA
                     GTYTVTLTATNAYGSDSETKTNYITVTTETAQCDDFNDGNYTGWSNKLGTWSAANYYL
                     KGNSTTSNARLTSPFGTFSTATITCKVRMNTGRTQRNTRIIFAYTNSSNYRYVQFDDY
                     YNRVYVGEILSGRQYTRAYASYTFSTGVWYDVTVSVAASGTVSMMVGSTSIGSYTFSA
                     RAGLVGVGYTKSNSDFDDFCVNAAASWAEAPAAPAELVPAGFELAQNYPNPFNPTTTI
                     SFTLPTPGDVTLEVFNVLGQRVAVLASGPYGAGTHHVEWNASGVSSGVYLYRLSAGEF
                     TETRKMLLVK"
     gene            complement(4353..5831)
                     /locus_tag="PK112_00055"
     CDS             complement(4353..5831)
                     /locus_tag="PK112_00055"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HPC10473.1"
                     /translation="MAWQGPLDKIDAWRYQIPSSFASPVIREKGLRMRVPGLIFTDEA
                     MLEAIRRDDSLFQVANVATLPGIVEKSIAMPDIHHGYGFAIGAVAAFDGAAGVISPGG
                     VGYDINCGVRLMRTDLHLGDVLPRIETLVDTMFANVPSGVGSSGRISLSEREVDEVLA
                     TGARWAVSREYGLAEDLEAMEEGGCLEESDPSVISKNAKARGHDQLGTLGAGNHFLEI
                     QRVDAIYEPDAARVFGLEEVGQIAVMIHTGSRGCGHQICTDYLESMRRASRKYGIALP
                     DQQLMCAPVDSPEARQYFLAMNAAANFAWCNRQVIMHWVRESFEAVFGQPADRLGLRL
                     VYDIAHNMAKREQHDIGGKKRWVYVHRKGATRAFGPGHRDVPEKYRAVGQPVIIPGDM
                     GTASYLLVGTAQAMRETFGSSCHGAGRRLSRTASIKEHRAEDVIRKLREGGIYLRAKS
                     KRVVAEEAPGAYKSVDEVVAISHHAGITRKVARLTPVGVVKG"
     gene            complement(5885..6307)
                     /locus_tag="PK112_00060"
     CDS             complement(5885..6307)
                     /locus_tag="PK112_00060"
                     /inference="COORDINATES: protein motif:HMM:NF014058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HPC10474.1"
                     /translation="MKRFEFLDHTADVILQGHGDTLAEAFAAAADGLFAVITDLDRIA
                     PTLDLDVAVEAIDREGLLVAFLSELIIRHEVDRIVAKDFAVVLEGDTRLAARGRGEKF
                     DERRHECGMHVKAVSYHMIEVVAGGPGRPARVRVVLDV"
     gene            complement(6358..7200)
                     /locus_tag="PK112_00065"
     CDS             complement(6358..7200)
                     /locus_tag="PK112_00065"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HPC10475.1"
                     /translation="MRLIFHPTCLTYHLPGHPETPRRVRLAWEHLSRQYETVPPEPAG
                     DEDILTVHTPRHLEMVRTGQFLDWDTPALPFIFDHALLAAGGALTAMRLALDGVAAIS
                     LLRPPGHHAGPDSVAGFCYFNNIAIAAARALEQVDRVAIVDFDCHHGNGTQDIFLGRD
                     RVVYLSLHQSPFYPGTGLFSERNCFNYPLRMGADEEMWLRALDDGLAKVRAFGPDLIA
                     VSAGFDLYKLDPFSFIPLSHEAFRAAGERIGALGRPVFSVLEGGYSDGLGRCAAAYLE
                     GLAG"
     gene            complement(7264..8223)
                     /locus_tag="PK112_00070"
     CDS             complement(7264..8223)
                     /locus_tag="PK112_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family protein"
                     /protein_id="HPC10476.1"
                     /translation="MKYRRVGRSGLKVSEIALGAWLTYGGPVSASQTAPIVRRAIAHG
                     VNFIDLADIYAWGAAEETLGRILKEFRRADLVLSSKLFWPMSDNPNDRGLSRKHIRES
                     IEGTLRRLGTDYLDIYFCHRFDPETEVEETVRAMDDLVRQGKIIYWGTSVWEAAQIER
                     AVGLARDYRAYAPIVEQPRYHLLDRHIEPEIMPVCAHNGMGLTVWSPLAQGLLTGKYN
                     AGIPDGSRGATSMWLKPSLTEANIAAVRRLSELAASLDITVARLALAWVLRRPEISCA
                     IIGATAPEQVDENVKAADTVLSAETLAAIEQILGNDPAARTQA"
     gene            8363..9103
                     /gene="ubiE"
                     /locus_tag="PK112_00075"
     CDS             8363..9103
                     /gene="ubiE"
                     /locus_tag="PK112_00075"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: protein motif:HMM:NF001244.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HPC10477.1"
                     /translation="MAAEDHADKTADPIPEKGESPQMFDRIAHRYDLVNRVLTLRLDV
                     RWRKKLAAMFPDRDNLAVLDVACGTGDVLTIGLKQRKNVSLGVGLDPAARMMARGRDK
                     ARECGLAERIAFVRGDALRLPFADGSFDVVTISFGIRNVTDVPRALAEMHRVLAPGGR
                     VLVLEFSFPDNRALRAAYLVYLRHILPRIGAAISGDAAAYRYLDRTVESFPYGEAFCD
                     LLRRAGFAAVRPRPLTFGVATVYSGEKK"
     gene            9132..10517
                     /locus_tag="PK112_00080"
     CDS             9132..10517
                     /locus_tag="PK112_00080"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HPC10478.1"
                     /translation="MMPSVFKDSDTHTPAGAAAHLAEEIARRAAHPRAGSPHVERCEV
                     PLAAMEPAAWLAAQRQSERIFWSDREDRSAVAGVGAAAVEFRAVCADPHDLMGRIREL
                     IGDAEGVRCYGGIRFDSDREQADHWRQFGGARFVLPRFELAARGGAAVLAVNLRFPED
                     RGQVSRIIEDLAQLRFAPADPGIPPAGVRRRVDTPGREDWLDIVGAAVDEINAGRMDK
                     IVLARESRCELGSPADPFRLTARLAASAPGCFVFAFKPPDGAAFLGATPERLYRREGR
                     RITSEALAGTRRRGETPAEDALLGEKLLTSRKEAQEHACVADAVRGALAALCREAAEE
                     KGRELVKLARVQHLATRFAGVLRDGVGDADILARLHPTPAVGGAPTATARQRIRELEA
                     FDRGWYAGPVGWIGADSAEFAVAIRSALAAGDTLRLYSGAGIVGGSHPDREWDEIENK
                     IDNYLKLLAGP"
     gene            10514..>10954
                     /locus_tag="PK112_00085"
     CDS             10514..>10954
                     /locus_tag="PK112_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006670476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HPC10479.1"
                     /translation="MNSRFPNLNSLWAEAAVEELVRCGVRLFCLCPGSRSTPLTAAAA
                     RHEAARKIVHFDERGAAFCALGYARATEKPAAVVVTSGTAAANLYPAVIEAAMDGVPL
                     LVLTADRPPELRASGANQTIDQTKLYADYPRWFFDLPCSSRETPP"
BASE COUNT         1839 a         3548 c         3789 g         1778 t
ORIGIN      
        1 cggaccggtc cgccagtcgc gggtgttcat gacctacggc gggtcgttcg agaaccttct
       61 cacgctcgcc cacgaactcg gccacgccta ccaccagcag gtcatcaccg agaaaccgta
      121 tttcgcgcaa cagtacccca tgccgctggc cgagacggct tcgattttct ccgaaacgct
      181 ggtgaccgat gcggccctgg gccaggcggc cgacgccggg gaacggctca tgctgctgga
      241 ccagaaactc cagtcggcct atgtgatgtt taccgacctg cactgccggt acattttcga
      301 ccgagccttt tacgcggaac gccggaacgg gatggtcccg gcggaacggc tgtcggagat
      361 gatggtgacg gcgcagaagg aggcgtttgc gggaatgctg gacgattcgg ggtgccaccc
      421 gctcttctgg gcatccaaac tgcacttcta catgacctcg gcgcccttct acaatttccc
      481 ctacgtgttc gggttcctct ttgccggcgg cgtctacgat cgggccaagc gggaggggaa
      541 gaagttcgcg aaggactacc gcgcgctcct ggcggacacc ggcagcatga cctgcgaaga
      601 gctcgcccgg actcacctgg gggtggatct ggccgacgag ggtttctggc gcagcgcggt
      661 cgaccgggcg ctggccgagg tcgaggcgta tgtgaagttg gcgaagtcga tgtaggaggc
      721 gtcggcgcgg attggcgccg accgggtccg ggggggccgg tcggttccgt gcggcgcgcg
      781 atgtgtgcgg aatacgaata gagcggcgcc tgcccgccgg aggctgagag cggagggctc
      841 tctcggagaa ccgtatgatg gaaaatgact tcactgtcga agtgctggtc gagatcccca
      901 aggggagccg gaacaagtac gagtacgaca aggcgcgcgg gctgttccgt ctcgatcgca
      961 tgctgttttc ggcggtgcac tacccgagcg attatggttt catcctcaat tcgctggggg
     1021 gcgaccgcga cccgctcgat gctctcgtgc tcacctggga gccgactttc ccgggatgcc
     1081 tgatcgatgc ccgtccggta gggctgttcc gcatgcagga cgaaaagggc ccggatgaga
     1141 agattttgtg cgtgccgatc cacgacccga tgtggaacag gatccgggcg ctggaggagg
     1201 tcccgccgca cctggtgaaa gagatcgagc atttcttcca ggtatacaag gagttggagg
     1261 agaagaagac ggcggtcgag gggtgggagg accgggcggc cgcggagcgc gtggtgcgcg
     1321 aggcgcagga gcgctggcgc aaagccggcg agcacgacta ggcccgccgc gcggggggaa
     1381 cctggaaaag ggggtccgct tttttttatt gactcgggcg cgcccgccct gtattttttc
     1441 agtaatcaca acgactatag gctgagtcag ccctttgttt tcgcgtatgg tgcggatcat
     1501 catgcaatct gcccgtggac tctcattgca tgtgaggagg tggtgcgccc gacagacact
     1561 attcccttgt tcgcaaccgt cacttgatta aacccggctg cccggagcaa agggtctcct
     1621 ttgcgggcag cgttcctccc aatcgaagaa ggagtcaaac catgagagca gtgttcgtgc
     1681 tggtgatgct gactgtcctt tgcggtttcg tcttcaccga ggattttcgg gtcgggcagt
     1741 catgcgtccc gccgtccgga gatgagtacg ttccggacga gatagttgtg aagttcaagg
     1801 acggcgtggg ccgccaggcg gtcagtgccg cgctcgccga cctcggggcc accacgatct
     1861 caaccaaccg ccagatcggt ttccaccagc ttcggatccc ggcggggacg acggttgagg
     1921 agatggtggc ggctttcggc gcccgtcccg atgtcgagta tgccgagcca aactatatcg
     1981 cccacgcctt catgacgccg aacgatccct actacacgta ccagtggcac atgccgctga
     2041 tcaagatgcc ggcggcgtgg gaccagacca ccggctcgcc cagcgtgatc gtggcggtgg
     2101 tcgactgcgg ggtcgcctac gagacctacg gcagcttcac gcaggccccg gacctggccg
     2161 ggacgagctt cgtgcccggc tatgatttca tcaacaacga cagccacccc aacgacgact
     2221 gcgcccacgg cacgcacgtg gccggcacgg tcgcccagac caccaacaac aacctcggcg
     2281 tgacgggcgt ggccttcaac tgcagcatca tgccggtgaa agtgctcgat gcctccggca
     2341 gcggttcgta cacggcgatc gccaacgggg tcacgttcgc cgccgacaac ggcgcccacg
     2401 tgatcaacat gagcctgggc ggatcctaca actcgtccac cctccagaac gcggtcgtct
     2461 acgcctacaa caagggcgtg acgatcgtct gcgccgcggg caacgccggc acctccacgc
     2521 cccagtatcc ggccgcctac tcccagtgca tctccgtctc ggccgtccgc tacaacaaga
     2581 cgtacacgtc gtacacgagc tacggctcga ccatcgacat ctgtgcgccc ggcggcgacg
     2641 tgagcgtcga ccagaacggc gacggctatg ttgacggcgt gctgcagcag acgcatgacg
     2701 ggaccaacta cgggacgttc gggtactact tctacgaggg gacgtcgatg gcgtcgccgc
     2761 acgtggccgg tgtggccgcc ctgctgatcg ccaagaacgg cggctcgatg gccccggccg
     2821 atgtgcgggc ggccctgcag aacacggccg aggatctcgg cgccgccggg tgggaccagt
     2881 actacggcta cggcttggtg gacgccaacg ccgccctgct gtcgatctcg accaacaacc
     2941 cgccagtggc ggctttctcc ggcacgccga cttcggggac ggcgccgctg acggtcgtgt
     3001 tcaccgacgc ctcgaccaac aatcccacga gctggtcgtg gaatttcggc gatggcggca
     3061 cgtcgactgc ccagaacccg acccacacct acgcctcggc gggaacgtac acggtgacgc
     3121 tgacggcgac caacgcgtac ggttcggact ccgagaccaa gaccggctac atcacggtgt
     3181 cggcgccgcc gacctacccg ccggtggcgg ccttctccgg cacgccgact tcggggacgg
     3241 cgccgctgac ggtcgtgttc accgacgcct cgaccaacac tcccacgagc tggtcgtgga
     3301 atttcggcga cggcggcacg tcaaccgccc agaacccgac ccacacctac acatcggcgg
     3361 ggacgtacac ggtgacgctg acggcgacca atgcgtacgg ttcggactcg gagacgaaga
     3421 ccaactacat tacggtcacg accgagaccg cgcagtgcga cgactttaac gacggcaact
     3481 acaccgggtg gagcaacaag ctcgggacct ggtcggccgc gaactactac ctgaagggga
     3541 actcgacgac ctcgaatgcc cggctgacct cgcccttcgg gacgttctcg acggcgacga
     3601 ttacgtgcaa ggtgcgcatg aacaccgggc gcacgcagcg caacacccgc atcatcttcg
     3661 cctacaccaa cagctcgaac taccgctacg tgcagttcga cgattactac aaccgggtgt
     3721 acgtcggcga gatcctcagc gggcgccagt acacgcgggc gtatgcgagc tacactttca
     3781 gcaccggggt ctggtatgac gtgaccgtgt ccgtggctgc gagcgggacg gtctcgatga
     3841 tggtcgggtc gacgtcgatc ggctcctaca ccttctcggc ccgggccggc ctggtgggag
     3901 taggctacac caagtccaac agcgacttcg acgacttctg cgtcaacgcc gcggcgtcct
     3961 gggcggaggc cccggcggcg ccggcggagc tcgtcccggc cgggtttgaa ctcgcccaga
     4021 actaccccaa cccgttcaac ccgacgacga cgatcagctt cacgctcccg accccgggcg
     4081 acgtgacgct cgaagtgttc aacgtgctcg gtcagcgggt ggcggtgctg gcgagcgggc
     4141 cgtacggggc cggcacgcac cacgtcgagt ggaacgcctc cggcgtgtcc agcggcgtgt
     4201 atctgtatcg gctctcggcc ggcgagttca cggaaacccg gaagatgctg ctggtgaagt
     4261 agcatcgtcc ggacaggaat tcggcagacg ccatgcgggc gggtcggtcg acccgcccgc
     4321 tttatattgg gcggagcgcg tccgcgccgg gtctatccct tgacgacccc gaccggagtc
     4381 aggcgggcga ctttgcgggt gatgccggcg tggtgggaga tggcgacgac ctcgtcgacg
     4441 ctcttgtagg cgccgggcgc ttcctcggcg acgacccgct tgctcttggc ccggaggtag
     4501 attcccccct cgcggagttt gcggatcacg tcctcggctc ggtgctcctt gatcgaggcg
     4561 gtgcgcgaca gccgccgccc ggcgccgtgg caggaggagc cgaaagtctc ccgcatggcc
     4621 tgggccgtgc cgaccaggag gtagctggcg gtgcccatgt cgccggggat gatcacgggc
     4681 tggccgaccg cccggtattt ctccgggaca tccctgtgcc ccgggccgaa ggcccgggtg
     4741 gcccctttgc ggtggacata cacccagcgc ttcttcccgc cgatgtcgtg ctgctcgcgt
     4801 ttggccatgt tgtgggcgat gtcgtacacg aggcgaaggc cgagccgatc ggccggctgt
     4861 ccgaaaaccg cctcgaacga ttcccgcacc cagtgcatga tcacctggcg gttgcaccag
     4921 gcgaagttgg ccgccgcgtt catcgccaga aaatactgcc gggcctcggg ggaatcgacc
     4981 ggtgcacaca tgagctgctg gtcgggcagc gcgatgccgt atttccttga ggcccggcgc
     5041 atgctctcca gatagtcggt gcaaatctgg tgtccgcagc cgcgcgaccc ggtgtggatc
     5101 atgaccgcga tctgccccac ttcctccagg ccgaacaccc gcgcggcgtc cggttcgtag
     5161 atggcgtcga cgcgctggat ttcgagaaag tgattcccgg cgccgagagt gccgagttgg
     5221 tcgtggccgc gcgccttggc gttcttcgag atcaccgacg ggtcactctc ctccagacac
     5281 cccccttcct ccatcgcttc gagatcctca gcgaggccgt actcgcggct gaccgcccac
     5341 cgggcgccgg tggccagcac ctcatcgacc tcgcgctccg acaggctgat tcttccggag
     5401 gaacccacgc cggaggggac gttggcgaac atcgtgtcga ccagcgtctc gatccgcggg
     5461 aggacgtcgc caagatggag gtcggtgcgc atgaggcgca cgccgcagtt gatgtcgtac
     5521 ccgaccccgc ccggggagat caccccggcc gcgccgtcga acgccgcgac cgccccgatg
     5581 gcgaacccgt aaccgtggtg gatgtcgggc atggcgatcg acttctcgac gatgcccggg
     5641 agggtggcga cgttggcgac ctggaagagc gagtcgtcgc ggcggatcgc ctcgagcatc
     5701 gcctcatcgg tgaaaatcag ccccggcacg cgcatccgca gccccttctc cctgatcacg
     5761 gggctggcga acgacgacgg gatctggtaa cgccacgcat cgattttgtc cagcggaccc
     5821 tgccatgcca tagcacactc cgatttccgg cggcgccggg cgcatttccc ggccgcgggg
     5881 atcctcacac gtcgaggacg acccgcaccc gggccggccg gcccgggccg cccgcgacga
     5941 cttcgatcat atggtacgac accgccttca cgtgcatccc gcactcgtgg cggcgctcgt
     6001 cgaacttctc cccccgcccg cgcgcggcga ggcgggtgtc cccctccaac acgaccgcga
     6061 aatccttcgc cacaatccgg tcgacctcgt gccggatgat gagctcgctg aggaaagcca
     6121 ccagcagccc ctcgcgatcg atcgcctcga ccgcgacgtc gaggtcgagt gtcggtgcga
     6181 tccggtcgag atcggtgatc accgcgaaca atccgtcggc cgcggcggcg aatgcctccg
     6241 cgagcgtgtc gccgtggcct tggaggatga cgtcggcggt gtggtcgaga aattcgaacc
     6301 gcttcatacc gtatagtatg ccgggcggcg gcggccgcaa ggacaattgc ggccggttta
     6361 cccggccagc ccttcaagat aagcggcggc gcaccggccg aggccgtcgg agtagccccc
     6421 ctccaggacg ctgaacaccg gtcggccgag cgcgccgata cgctccccgg cggcccggaa
     6481 cgcttcgtgc gacagcggga taaacgaaaa cggatcgagc ttgtagaggt caaacccggc
     6541 cgacaccgcg atcaggtcgg ggccgaaagc ccgcactttc gccaggccgt catccagcgc
     6601 ccgcagccac atctcctcgt cggcgcccat ccggagcgga tagttgaagc agttccgctc
     6661 ggaaaacagc ccggtgccgg ggtagaacgg gctctggtgg agggagaggt acacgacccg
     6721 gtcgcggccg agaaaaatgt cctgcgtgcc gttgccgtgg tggcagtcaa aatcgacgat
     6781 ggcgacgcgg tccacctgtt cgagggcccg ggcggcggcg atggcgatgt tgttgaaata
     6841 gcagaacccg gcgaccgagt ccgggccggc gtggtggccg ggcggacgga ggagggagat
     6901 cgccgcgacc ccgtcgagcg caagccgcat cgctgtcagg gcgccgcccg ccgcgagcag
     6961 ggcatggtcg aagatgaacg ggagggcggg cgtgtcccag tcgagaaatt gcccggtccg
     7021 caccatctcc aggtggcggg gcgtgtggac ggtcaaaata tcctcgtcgc cggccggttc
     7081 cggcggcacc gtctcgtact gccgggacag gtgctcccag gcgagccgca cgcgccgggg
     7141 cgtttcggga tgccccggca ggtggtaggt cagacaggtc ggatggaata tcagccgcat
     7201 ctgtgccaat ccgttcgcgg ccgcgcgccc ggaccccgca ccctctcgga ccggaggggg
     7261 atgttacgcc tgggtgcggg ccgccgggtc gttgccgagg atctgttcaa tcgccgcgag
     7321 ggtctcggcc gacaagacgg tgtcggccgc tttcacattc tcatcgacct gttccggcgc
     7381 ggtggcgccg ataatggcgc aactgatttc cggccggcgc agaacccagg cgagggcgag
     7441 ccgggccacg gtgatgtcca gcgacgcggc gagttcgctg agccgccgga cggcggcgat
     7501 gttggcctcg gtcaggctcg gcttgagcca catgctcgtg gcgccgcggc ttccgtccgg
     7561 tatccccgcg ttgtacttgc cggtgagcag cccctgggcg aggggggacc acacggtcag
     7621 gcccatgccg ttgtgggcgc acaccggcat gatctccggc tcgatgtgac ggtcgagcag
     7681 atggtagcgg ggctgttcca cgatcggcgc gtaggcgcgg tagtcgcggg ccagaccgac
     7741 cgcccgctcg atctgggccg cctcccacac cgaggttccc cagtagatga tcttcccctg
     7801 ccgcaccagg tcatccatgg cgcggacggt ttcctcgacc tccgtctccg gatcgaagcg
     7861 gtggcagaag tagatgtcga ggtagtcggt gccgagacgg cggagcgttc cctcgatgga
     7921 ttccctgatg tgtttgcgcg agaggccccg gtcgttgggg ttgtcggaca tcggccagaa
     7981 gagcttgctg gacaggacca ggtcggcgcg gcggaattcc ttgagaatac ggccgagggt
     8041 ttcttcggcc gcgccccagg cgtagatgtc ggcgaggtcg atgaaattga cgccgtgggc
     8101 gatcgcccgg cggacgatgg gcgcagtctg tgaggcggag accggcccgc cgtaagtcaa
     8161 ccaggcccca agagcgatct ccgagacttt cagcccgctg cggccgactc gacgatactt
     8221 catgaacgct gatcctttcg cgttgcggca gccttttggg caaggtacga aaaaggaccg
     8281 cgcgaggcaa tcgggtttga gggagcggag ggattgcgta tattgcggcg gtcggaacgg
     8341 atcggccgga aagcggatag aaatggcggc ggaagatcac gcggacaaga cggcggaccc
     8401 gatccccgaa aagggggagt cgccccagat gtttgaccgg atcgcgcacc ggtacgacct
     8461 ggtcaaccgc gtgctgacac tccgcctgga tgtccgctgg cggaagaagc tggcggcgat
     8521 gtttcccgac cgggacaatc ttgccgtgct cgacgtcgcc tgcggcaccg gggatgtact
     8581 gacgatcggg ctgaagcagc ggaagaatgt gagcctgggg gtggggctcg atccggcggc
     8641 gcgcatgatg gcccgcggcc gggacaaagc gcgggaatgc gggctggccg agcggatcgc
     8701 attcgtgcgg ggcgatgccc tgcgcctgcc gtttgccgac ggaagttttg acgtcgtgac
     8761 gatcagtttc gggattcgca atgtgaccga cgtgccgcgg gcactggccg agatgcaccg
     8821 ggtgctggcc cccggcgggc gcgtgctggt gctcgagttt tccttccccg acaaccgcgc
     8881 cctccgggcg gcctacctgg tctacctccg gcacatcctc ccgcgcatcg gcgcggcgat
     8941 cagcggggat gcggcggcct accgctacct cgaccggaca gtggagtcgt ttccctacgg
     9001 cgaggcgttc tgcgacctcc ttcgccgggc cgggttcgcg gcggtccggc cgcggccgct
     9061 gacgttcgga gtggccactg tttacagcgg ggagaaaaag tgatatagtg cggaaccaac
     9121 ggtgcaggcc catgatgccc tcagtattca aagacagcga cacgcacacg cccgccgggg
     9181 cggcggccca tctggccgaa gagatcgccc ggcgggcggc gcatccgcgg gcgggcagcc
     9241 cgcacgtgga gcggtgcgag gtccctctcg ccgcgatgga accggcggcc tggctcgcgg
     9301 cgcagcggca gagcgagcgg atcttctgga gcgaccgcga ggaccgctcg gccgtcgccg
     9361 gggtgggcgc cgcggcggtg gaattccgcg cggtctgcgc cgatccgcat gacctgatgg
     9421 ggcggatccg ggaactgatc ggcgacgccg agggcgtgcg gtgctacggc ggaatacggt
     9481 tcgacagcga ccgggagcag gccgatcact ggcggcagtt cggcggagcg cggtttgtgc
     9541 tcccccgctt cgagctcgcc gcccgcggcg gggcggccgt gctggcagtc aatctgcggt
     9601 tccccgagga ccgcgggcag gtttcccgca tcatcgaaga cctggcgcag ttgcgcttcg
     9661 cgccggcgga ccccggtatc ccgccggccg gcgtgcgccg gcgggtcgat acgccgggcc
     9721 gagaggattg gctggacatt gtcggagcgg cggtggacga aatcaacgcc gggcggatgg
     9781 acaagatcgt gctcgcgcgc gaaagccgct gtgagctggg cagtccggcg gatccgttcc
     9841 gcctgacggc ccggctggcg gcctcggcgc cgggctgttt cgtcttcgcc ttcaagccgc
     9901 cggacggcgc cgccttcctg ggagcgacgc cggagcggct gtaccgccgg gaggggcggc
     9961 ggatcacgag cgaggctctg gccggaaccc ggcggcgcgg ggagacgccg gcggaggacg
    10021 ccctcttggg cgagaaactc ctgaccagcc ggaaagaagc gcaggaacac gcctgcgtcg
    10081 ccgacgcggt gcgcggggcg ctcgcggcgc tgtgccggga ggcagccgag gagaagggac
    10141 gggaactggt caaactggcg cgcgtgcagc acctcgcgac ccgtttcgcg ggcgttctca
    10201 gagacggcgt cggggacgcg gatattctgg cgcgcctcca cccgacgccg gcggtcgggg
    10261 gggcgccgac cgcaaccgcc cggcagcgga ttcgcgaact cgaggctttc gaccggggct
    10321 ggtatgccgg accggtcggt tggatcgggg cagacagcgc ggaattcgcg gtcgcgatcc
    10381 gctcggcgct ggcggccgga gacacgctgc ggctctattc gggagccggg attgtcggcg
    10441 gttcgcatcc cgacagagaa tgggacgaga tcgaaaataa gatcgataac tacctgaagc
    10501 tgctggcggg gccatgaaca gccgatttcc gaatctgaac agcctctggg cggaggccgc
    10561 ggtcgaagaa ctggtgcgct gcggggtgag gttgttctgc ctctgccccg gctcgcggtc
    10621 gacgccgctg acggcggcgg cggcgcggca cgaggcggct aggaagatcg tccatttcga
    10681 cgagcgcggg gcggccttct gcgcgctcgg ctacgcgcgg gcgacggaaa aaccggcggc
    10741 cgtggtcgtg acctcgggaa ccgcggcagc caacctctac ccggcggtga tcgaggcggc
    10801 gatggacggc gtgccgctgc tggtcctcac ggcggaccgc ccgcccgagc tgcgggcaag
    10861 cggcgcgaat cagacgatcg accagacgaa gctctacgcc gattatcccc ggtggttttt
    10921 cgatctcccc tgctccagcc gggagacacc gccg
//
LOCUS       DAOOKS010000003        10081 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_7651_length_10081_cov_3.506683, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000003 DAOOKS010000000
VERSION     DAOOKS010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10081
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7651_length_10081_cov_3.506683"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..996
                     /locus_tag="PK112_00090"
     CDS             <1..996
                     /locus_tag="PK112_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HPC10480.1"
                     /translation="AFVCGEETALIASIEGRRGMPRSRPPFPAIAGLHGKPTIINNVE
                     TLGTLPHILRHGAAWYRQYGTQGNYGTKTFSLVGKVRRPGLIEVPLGMTLRQIIFDIG
                     GGTRKKFKAVQTGGPSGGCLSEEFLDTPVDYESLTAAGSIMGSGGLIVMDEDTCVVDI
                     ARYFLDFTQKESCGKCTPCRVGSRHMVEILERITLGQAAPEDLIRLQVLAETVKRGSL
                     CGLGQTAPNPALTTLRYFRPEFLLHVTEKHCPASVCRELIEYRVVPEKCTGCQRCVSV
                     CPTGAITGPRSETHNLDPDKCIKCRACYEICRFDAIAGDAIIIRSKRSVPNELTP"
     gene            980..3490
                     /locus_tag="PK112_00095"
     CDS             980..3490
                     /locus_tag="PK112_00095"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPC10481.1"
                     /translation="MNSPHDPVTVTIDSRSLSAARGATILEIARAAGLYIPTLCAHKD
                     LTPYGGCRMCIVEVDGMRGFPTACTTPAESGMVIRTHTAQLHEIRTEILRLILSEHPC
                     SCLVCDEQDECRQYSATIRKAGVTTGCRYCPNDGQCELQEVAARLGITEIGYPVYYRN
                     LPVHKDDPFFDRDYNLCILCGRCVRMCQEIRTAGTLTFLRRGPQTVIGPAFDRTHLEA
                     GCEFCGACVSVCPTGALAEKARKWKGAAEREVTTTCPFCGVGCQVRLQVKKDEVVGAL
                     PAEDDLVNRGQLCVKGRFCIPEIVSHPRRLRHAYKLRDGVLVDIGWEEAATLAAERLT
                     ACPPEEFGLLVSANCSNEDLYVAQKFARAVMRSNNIDTTFRLAYGASCAPYVEHLRAP
                     ATLEDLRRAEAVIAIGLDTRFGRSVIGAELRKAFRRGARIVTIHHRPHDLAQIAAHWL
                     RPAAGQEGELLRELMAATAPAARGGRGKTTGGADIAAAAGTIRESGRTVILLGPMLLD
                     RTDGPDLLRAVHAWAAALGAAVMPLPSQNNFLGALLMGAVGELLPGGAAWADTAARDR
                     LARRWQSALPAEGAQWNSSLVGRGPRRKVLYLLGEVIPNPRAGAETVIFQNIFHPPDL
                     TGADLVLPAAAFSETDGTVINGEGRVQRLHRALDPPGDAAPDWEIVCRIARAMGAAGF
                     AFGSAREIHEEIATLIEGFDAFDAPARAPHRWPLAECDLPAAASAPRSDGSATNPFLL
                     LTTNLEHTHRGFAMADRVAGLSRLYPLRVVEMNPVDARTMGIADGETVAVTAEGLDRC
                     WPVCVTAAQPEGLLHVVLPPGELDGANPRRVQVRKCHV"
     gene            3483..4325
                     /locus_tag="PK112_00100"
     CDS             3483..4325
                     /locus_tag="PK112_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HPC10482.1"
                     /translation="MYKVIDNRMVVPNIHMLRVEAPHVSREARPGQFVIVRAEDDGER
                     IPLSISDWDARDGTIVVVYKNVGATTNRLSNVRPGESLLTVVGPLGKETEIDTYGTVL
                     CVGGCYGIGSIYPIARALRERGNRVIVVGEARSSYLTFWDDKLRAAAHRVVWITRDGT
                     RGLRGHVPRLSEVIGQESGPINRAIVNGCTFLMKRVSDMTRPLGIKTIVSLNPIMIDG
                     TGMCGVCRVSVGGATRFACVDGPDFDGHEVDWTELLQRRKSYLTEEIIPQSTSRCEEH
                     AGNA"
     gene            4343..5752
                     /gene="gltA"
                     /locus_tag="PK112_00105"
     CDS             4343..5752
                     /gene="gltA"
                     /locus_tag="PK112_00105"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HPC10483.1"
                     /translation="MTEEPQRKKIDPHRREMPKQAAEVRRRNFHEVALGYPIATAVAE
                     AKRCLQCKKPKCVSNCPVEIDIPGFIQHIADGDFAGGTRVLKEKNCLPAVCGRVCPQE
                     EQCEKTCLLEKKGCAVAIGRLERFLADWEAAQGDPELPSIPAPTGRTIAVVGGGPAGL
                     TVAGDLIKLGHRVTIFEALHKMGGVLMYGIPEFRLPKAIVAREVNYLRMLGVEIITDF
                     VVGKTRPLARLLEEFDAIFIGAGAGLPWFMQIPGENLNGVYSANEYLTRMNLMKGYLF
                     PDSPTPIKGHTRVAVIGGGNVAMDCARTALRLGAETRIIYRRSRQELPARLEEVENAE
                     EEGVILDLLTLPVKYIGDSDGWVREIECIRMELGEPDASGRRSPVPVKDSNFRVAVDA
                     VVCAIGNSPNPLIPTTTAGLEIGKKGNIVADDATGRTSLARVWAGGDVVTGAATVILA
                     MGAGRKAARSMHEFLMAPR"
     gene            complement(5785..6720)
                     /locus_tag="PK112_00110"
     CDS             complement(5785..6720)
                     /locus_tag="PK112_00110"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019364575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HPC10484.1"
                     /translation="MVNSYGNVLQAIGDTSMVQLRRVAPPGAAAIVAKLEWENPTGSM
                     KDRAALAMIARAEADGRLKSGDTVVEYTGGSTGISLALVCLARGYRLRIVSSDAFSRD
                     KLRHMAALGAELTLFPSEGGLTTKKRIRDMIEAARELSRQPRTYWTNQLSNADSIEGY
                     RSLGEEIWTQTGGRVDAFVHCVGTAASLRGVAAVLKERRPAIRVAAVEPAESAVLSGG
                     RPGPHKIEGVGIGYTPPLWDPGLVDEIVAVGTAEAKAMARRLAREEGIFAGTSSGANV
                     LAAIAVARRLGPRATVVTLMADTGLKYVSTDVFEG"
     gene            complement(6791..8017)
                     /locus_tag="PK112_00115"
     CDS             complement(6791..8017)
                     /locus_tag="PK112_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10485.1"
                     /translation="MVKWPTLIGAFLALVVCAQPAHQENTVTDNLILNPREWGDRQST
                     GYLPVSGRLGQETVWQKAADAAGDSVLPHHLCLIGDRIAVWYGRRLDLRSAATGEPLW
                     SQETLENCSFEAQRDGLVAFDEHGAFRLLRYDGTYGERVRAPLYNGAGRVFVADASAD
                     AVFLCAQMHPGPTSTAGEKVVGPEIILAALNRGANELAWSVIRDGFLIAALISPDGRQ
                     ACLATRDRLLLAALPAAAAYDPAAELEVAVIEIPFDSVIACAYSHAGEILAVAEKSDN
                     PEGRRERALFVLSSAGEERRRLPLAGAGSLAQPPASFPDGDVCLSLGREIWRLRGGEK
                     LWVQKIPAEPGQILITLLKDGSLLAAAGKLLLQLSPEGEEISRRIVDGPITCRPVMDP
                     QGRVYVAGPGGVYCLR"
     gene            complement(8094..9998)
                     /locus_tag="PK112_00120"
     CDS             complement(8094..9998)
                     /locus_tag="PK112_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10486.1"
                     /translation="MSSPGAAAIAQAILAHYEQAMLVVDPATGFLKLYQPGADLNSAR
                     IPFARNLITEPAEDVKIVFFRDGFYPVGRRRTEPSPNWPANGFVVGARAAVRNDSDYH
                     CTWALQETRTEFASTGDFDLHYFHALHVDDEHPISFTISYASMSFMADTRAPAQFGPI
                     PTEAEVDNFRNQGCYRAMDVWNRKQLFMEEIAPSARTVIIRHFYFFDEREHFKPTAPA
                     GGWVNCNFDNQPSDNAADNFETLFAQQALRDAERDALGGKAKFLALVCRNENGDWGPA
                     YQWAIRDSANRPYSLLKLNKSCYQDTPTILNGISVTEHGDTWVPNTMAHELGHGTGQP
                     DEYIRRQFTPHSGANAGANANFIQHYIPYSMPFNNGSLMYWNCAPRLRYLWYPMRKIN
                     VESRRVGSPLRALLPDKTFVARLDRPSFVFTFHRKVTGGNPSVPAVKEDPIASEKQYE
                     LTSSPVRKLSLELYDVGRDESSRDTFHAAQGGRVYHAVLVVRVLMKIEWTGSWTDNRK
                     RDRIVEIEDAFKAWGGHYRLEGGTGDIRDIYIHFLPGFYASSDGDTESDKHYDVELTR
                     GAETAISTGFWNSSDLEVSRVITGAQLVDYFFNRASGQTGEQALHFLADWADGRLPGT
                     FTLHSWGGPP"
BASE COUNT         1582 a         3204 c         3681 g         1614 t
ORIGIN      
        1 gctttcgtgt gcggcgagga gaccgccctg atcgcatcga ttgagggccg gcgggggatg
       61 ccccgctcgc gcccgccctt cccggccatc gccggcctcc acgggaagcc gacgatcatc
      121 aacaatgtcg agacgctcgg cacccttccg cacattctgc ggcacggggc cgcctggtac
      181 cgccagtacg gcacgcaggg gaactacggc accaagacgt tttcgctggt gggcaaggtg
      241 cggcggccgg ggctgatcga ggtgccgctc gggatgacgc tccggcagat cattttcgac
      301 atcggcgggg gcacgcggaa gaagttcaaa gcggtccaga ccggcggacc gtcgggcggc
      361 tgcctctccg aggagttcct cgacacgccg gtggattacg agtcgctgac ggcggccggg
      421 tcgatcatgg gttcgggggg gctgatcgtc atggatgagg acacgtgcgt ggtggacatc
      481 gcccggtact tcctcgattt cacgcagaag gagtcgtgcg gcaagtgcac gccctgccgg
      541 gtcgggtcgc gccacatggt ggagattctg gagcgcatca cgctcggcca ggcggcgccg
      601 gaggacctga tccggctgca ggtgctggca gagacggtca agcggggctc gctgtgcggg
      661 ctcggccaga cggcgcccaa cccggcgctg acgacgctgc gctatttccg gccggagttt
      721 ctcctgcatg tgacggagaa gcactgtccg gcctcggtgt gccgcgagct gatcgagtac
      781 cgggtggtgc ccgagaaatg cacggggtgc cagcgctgcg tgagcgtctg tccgacgggg
      841 gcgatcacgg gtccgcgctc ggagacgcac aacctcgatc ccgacaagtg catcaagtgc
      901 cgggcgtgtt acgagatctg ccgtttcgac gccatcgccg gcgacgccat catcatccgg
      961 tccaaacgga gtgtgcccaa tgaactcacc ccatgacccc gtcaccgtca ccatcgacag
     1021 ccgttccctg agcgcggcgc gcggggcgac cattctggag atcgcgcgcg cggccggcct
     1081 ctacatcccg accctgtgcg cccacaagga cctcacgccc tacggcgggt gccggatgtg
     1141 cattgtcgag gttgacggga tgcgcggttt tccgaccgcc tgcacgacgc cggccgaatc
     1201 gggcatggtg atccgcaccc acaccgccca actgcacgag atccgcaccg agattctccg
     1261 cctgatcctg agcgagcatc cgtgcagctg cctggtgtgc gacgagcagg acgaatgccg
     1321 gcagtactcg gccacgattc ggaaggccgg cgtgaccacc ggatgccgct actgcccgaa
     1381 cgacggccag tgcgaactgc aggaggtggc ggcccggctc ggcatcaccg agatcggcta
     1441 ccccgtctac taccgcaacc taccggtgca caaggacgat ccgtttttcg accgcgacta
     1501 caacctctgc attctctgcg ggcggtgcgt gcggatgtgc caggagatcc ggacggcggg
     1561 gacactgacc tttctgcggc gggggccgca aacggtgatc ggtccggcgt tcgaccggac
     1621 gcatctcgag gccggctgcg agttctgcgg cgcgtgcgtg tcggtgtgcc cgaccggcgc
     1681 gctggcggag aaagcgcgca aatggaaagg ggccgccgag cgcgaggtca ccacgacgtg
     1741 cccgttttgc ggtgtcggct gccaggtgcg cctgcaggtc aagaaagacg aagtggtcgg
     1801 ggcgctgccg gccgaggacg acctggtcaa tcgggggcag ctatgtgtga aggggcggtt
     1861 ctgtatcccc gagattgtgt cccatccgcg gcggctccgg cacgcgtaca agctgcgcga
     1921 cggggtactg gtcgacatcg gctgggagga ggcggcgact ctggcggccg agcggctgac
     1981 cgcgtgccca ccggaggagt tcggcctgct cgtttcggcg aactgcagca acgaggatct
     2041 ctacgtggcg cagaaattcg cacgcgcggt catgcgttcg aacaacattg acacgacgtt
     2101 ccgcctggcc tacggggcga gttgcgcgcc gtacgtcgag cacctgcggg cgccggcgac
     2161 gctggaggat ttgcgccggg cggaggccgt gatcgcgatc gggctggata cgcgtttcgg
     2221 ccggtcggtg atcggggccg agttgcgcaa ggcgttccgg cggggcgcgc gcattgtcac
     2281 gattcaccac cggccgcacg atctggcgca gatcgccgct cactggctcc ggccggccgc
     2341 cggccaggag ggggagctgc tccgggagtt gatggccgcg acggctccgg ccgcgcgcgg
     2401 cgggcgggga aagaccacgg gcggagcgga tatcgccgcc gccgcgggga cgatcaggga
     2461 gtcggggcgg acggtgatcc tgctcgggcc gatgctgttg gaccggacgg acgggcccga
     2521 cctgctgcgg gcggtgcacg cctgggcggc ggcgctcggg gccgcggtga tgccgcttcc
     2581 ctcccagaac aacttcctcg gcgccctgct catgggcgcg gtcggcgagc tgctccccgg
     2641 cggggcagcc tgggccgaca ccgccgcccg ggaccgcctc gcccggcgct ggcagtccgc
     2701 gctgccggcg gaaggcgcgc agtggaacag ttccctggtc ggccgcggcc cgcgccggaa
     2761 ggtcctctac cttctggggg aggtgatccc gaatccgcgg gccggcgccg agacggtgat
     2821 attccagaac atcttccacc cgccggacct caccggggcc gacctcgtgc tgccggcggc
     2881 ggcgttcagc gagaccgacg gcacggtgat caacggggag gggcgcgtcc agcggctgca
     2941 ccgggcgctc gacccgccgg gggacgcggc gcccgactgg gagattgtct gccggatcgc
     3001 gcgcgccatg ggtgcggcgg ggttcgcgtt cggctcggcg cgggagatcc acgaggagat
     3061 cgcgactctg atcgaaggtt tcgacgcatt cgatgcaccc gcgcgggcgc cgcaccggtg
     3121 gccgctggcc gagtgcgact tgcccgcggc cgcgtccgca ccccggtcgg acggctcggc
     3181 gacaaatccg tttctgctat taaccaccaa cctcgagcac acccaccggg ggttcgccat
     3241 ggccgaccgg gtggccggtc tgtcgcggct ctatccgctg cgggtggtcg agatgaaccc
     3301 ggtcgacgcc cggacgatgg ggattgccga cggcgagacg gtggcggtta ccgcagaggg
     3361 gctggaccgg tgctggccgg tgtgcgtgac ggcggcgcag cccgagggtc tgctccacgt
     3421 ggtgctgcca ccgggcgaac tcgacggtgc gaacccgcgc cgcgtgcagg tgaggaaatg
     3481 ccatgtataa agtaatcgac aaccgcatgg tcgtcccgaa catccacatg ctccgggtgg
     3541 aggcgcccca cgtgtcgcgc gaggcgcggc cgggacagtt cgtgatcgtc cgggccgagg
     3601 atgacggcga gcgcattccg ctgtcgatat ccgactggga cgcccgcgac ggcacgatcg
     3661 tcgtcgtcta caagaacgtc ggcgccacca ccaaccgcct ctccaacgtc cggccagggg
     3721 aaagccttct cacggtcgtc ggtccgctcg ggaaggagac ggagatcgac acctacggca
     3781 cggtgctgtg cgtcggggga tgctacggga tcgggagcat ttacccgatc gcgcgggcgc
     3841 tgcgcgagcg gggcaaccgc gtcatcgtcg tcggcgaggc ccgcagctcc tacctcacgt
     3901 tctgggacga caagctgcgg gcggcggcgc accgggtggt ctggatcacg cgggacggga
     3961 cgcgcggtct gcgcgggcac gtgccgcgcc tgtcggaggt gatcgggcag gagtcgggcc
     4021 cgataaatcg cgccatcgtc aacggctgca cgttcctgat gaagcgggtg tcggacatga
     4081 cccgccccct cggcatcaag acgatcgtca gcctgaaccc gatcatgatc gacggcacgg
     4141 gcatgtgcgg cgtctgccgg gtctcggtcg gcggcgccac ccgcttcgcc tgtgtcgacg
     4201 gtccggactt tgacggccac gaggtcgact ggactgagct tctccagcgc cgcaagtcgt
     4261 atttgaccga agagattatt ccgcagtcga cgagccggtg cgaggagcac gccggcaacg
     4321 cgtaaccgag ggatgaggcg ccatgacaga agaaccgcag cgaaagaaga tcgacccgca
     4381 ccggcgggag atgcccaagc aggcggccga ggtgcgccgg cgcaactttc acgaggtggc
     4441 gctgggctac ccgatcgcga cggccgtggc cgaggcgaag cgctgcctgc agtgcaagaa
     4501 gccgaagtgc gtgagcaact gcccggtcga gatcgacatc ccggggttca tccagcacat
     4561 tgccgacggc gatttcgccg gcggcacgcg ggtcctcaag gagaagaact gtctcccggc
     4621 cgtgtgcggc cgcgtctgcc cgcaggaaga gcagtgcgag aagacctgcc tgctggagaa
     4681 gaagggctgc gcggtggcga tcgggcggct cgagcggttc ctcgccgact gggaggcggc
     4741 ccagggggat cccgagctcc cgtcgatccc cgcgccgacc gggcgcacga tcgcggttgt
     4801 cggcggaggg ccggcgggcc tgacagtcgc gggcgacctg atcaagctgg gccaccgggt
     4861 cacgatcttc gaggcgctcc acaagatggg gggggtcctg atgtacggga tccccgagtt
     4921 ccggctgccc aaagcgatcg tcgcgcgcga ggtgaactac ctgcgcatgc tgggcgtgga
     4981 gatcatcacc gatttcgtcg tgggcaagac gcggccgctc gcccgcctgc ttgaggaatt
     5041 cgacgcgatc ttcatcgggg cgggcgccgg actcccctgg tttatgcaga tacccggcga
     5101 gaacctcaac ggcgtctact ccgccaacga gtacctcacg cggatgaatc tgatgaaggg
     5161 gtacctcttc cccgactccc cgacgccgat caaggggcac acgcgggtgg cggtgatcgg
     5221 cggcggcaac gtcgccatgg actgcgcccg caccgccctc cgcctgggcg cggagacgcg
     5281 catcatctac cgccgctccc gccaggagtt gccggcgcgg ctcgaggagg tcgagaacgc
     5341 ggaggaggag ggggtgattc tggatttgct gacgctgccg gtgaagtaca tcggcgacag
     5401 cgacggctgg gtgcgggaga tcgagtgcat ccgcatggaa cttggcgagc cggacgcctc
     5461 cgggcgccgc tcgccggtgc cggtcaagga ttcgaatttc cgggtggccg tcgacgcggt
     5521 cgtgtgcgcg atcggaaaca gcccgaaccc gctgataccg acgacgacgg cggggctgga
     5581 gatcggcaag aaggggaaca tcgtggccga tgacgcgacc gggcgcacct cactggcgcg
     5641 ggtgtgggcg ggcggcgacg tggtgacggg tgcggcgacg gtgattctcg cgatgggagc
     5701 cggtcgcaag gccgcccgct ccatgcacga gtttttgatg gcgccgcgct aagcgcggcg
     5761 ctgtagccgg cggcggaccc tcccctatcc ctcgaatacg tcggtgctca cgtatttcag
     5821 ccccgtgtcc gccatcaggg tgacgaccgt ggcccggggg ccgagccgcc gggcgaccgc
     5881 gatggccgcg aggacgttcg cccccgacga agttcccgca aagatcccct cctcgcgcgc
     5941 gagccgccgc gccatcgcct tggcctcggc ggtgcccacc gcgacaatct catcgaccag
     6001 gccgggatcc cacaggggcg gggtgtagcc gatccccacg ccctcgatct tgtgcggtcc
     6061 cgggcggccc ccggagagga cggcggattc cgccggctcg acggccgcca cgcggatggc
     6121 gggtcggcgc tctttcagga cggcggcgac accgcgcagc gacgcggccg tgccgacgca
     6181 gtgcacgaag gcatcgaccc ggccgccggt ctgggtccag atctcctcgc cgagggaccg
     6241 gtagccctcg atgctgtcgg cgttgctgag ctggttggtc cagtaggtgc ggggttgccg
     6301 gctgagctcg cgggcggcct cgatcatatc gcgaatgcgc ttcttggtgg tcaggccgcc
     6361 ctcgctgggg aacagggtca gctccgcgcc cagggccgcc atgtgcctga gcttgtcccg
     6421 gctgaaagca tcggaggaga cgatgcgcag gcggtacccc cgggcgagac agacgagggc
     6481 cagcgagatg ccggtgcttc cgccggtgta ctcgacaacc gtgtcgccgg atttcagccg
     6541 gccgtcggcc tcggcccggg cgatcatggc cagagcggcg cggtctttca tgctcccggt
     6601 agggttttcc cactcgagct tggcgacaat ggcggcggcg cccggcggcg cgactctgcg
     6661 gagctgcacc atcgacgtgt cgccgatcgc ctgaagaaca ttcccgtatg aattcaccat
     6721 ggcttcgctt tcgcgcaggc ggcgggttcg cgcggcccga tgcgcgcaga cggccccggc
     6781 agatgagatc ctagcggagg cagtagacgc cgccggggcc ggcgacatag acgcggccct
     6841 gcggatccat gacaggccgg caggtgatcg gcccgtcgac aatccggcgg ctgatttcct
     6901 cgccctccgg ggacagttgc agcagcagtt ttccggcggc ggcgaggagg gagccgtctt
     6961 tcagcagcgt gatgaggatc tgtcccggct cggcagggat tttctgcacc cagagcttct
     7021 cgccgccgcg gaggcgccag atttcgcggc cgagcgagag gcagacgtcg ccgtcgggaa
     7081 aactcgccgg cggctgcgcg agacttcccg ccccggccag cgggaggcgg cggcgctcct
     7141 cgccggcgga cgacaggaca aagagggcac gctcgcgtcg gccctcgggg ttgtcggatt
     7201 tttcggccac cgccaggatc tcgccggcgt gcgaataggc gcaggcgatc acgctgtcga
     7261 acgggatctc gatcaccgca acctcgagtt cggcggccgg atcgtaggcg gccgccgccg
     7321 ggagggcggc gaggagcagc cggtcccggg tcgcgaggca agcctggcgg ccgtccggac
     7381 tgatcagggc cgcgatgaga aaaccgtccc ggatcaccga ccaggccagc tcgttggcgc
     7441 cccggttgag ggcggcgagg atgatctccg ggccgacgac cttctccccc gccgtactcg
     7501 tcggtccggg gtgcatctgg gcgcagagga agacggcgtc ggcggaggca tcagcgacaa
     7561 agacgcgtcc ggcgccgttg tacagcggcg cccggacgcg ctcgccgtaa gtgccgtcat
     7621 agcggagcag ccggaaggca ccgtgttcat cgaaggcaac aaggccgtct ctctgcgctt
     7681 cgaacgagca gttttcgagc gtctcctgcg accagagggg ttcgccggtg gccgcgctgc
     7741 gcaggtcgag gcggcggccg taccacacgg cgatgcggtc gccgatgagg cagagatggt
     7801 gcgggagcac ggagtcgccg gccgcatcgg ccgccttctg ccacacggtc tcctgtccga
     7861 gccgtccgga caccggcaga tagccggtgc tctggcggtc gccccactcg cggggattga
     7921 ggatgaggtt atcggtcacg gtgttctcct gatgagccgg ttgcgcgcac acgaccagcg
     7981 cgaggaaggc gccgatgagg gtcggccatt tgaccatagt tcccattcct ttcgaaggcg
     8041 gctggatgtt gacgacgcgg tatcattggt ttcgcctttg cggccggtcc cccttacggc
     8101 gggccgcccc aactgtgcag cgtgaacgtt cccggaaggc ggccatcggc ccaatccgcg
     8161 aggaagtgga gcgcctgctc gccggtctgg ccggaggcgc ggttgaaaaa gtaatcgacg
     8221 agctgggcgc cggtgatgac gcggcttacc tcgagatcgg aactgttcca gaagccggtg
     8281 ctgatggcgg tctcggcccc ccgcgtcagc tcgacgtcgt aatgcttgtc ggactcggtg
     8341 tcgccgtcgc tggaggcata gaagccgggc aggaagtgga tgtagatgtc gcggatgtcg
     8401 ccggtgccgc cctccagccg gtagtgtccg ccccaggcct tgaaggcatc ttcgatttcg
     8461 acgatgcggt cgcgcttgcg gttgtcggtc cacgatccgg tccactcgat tttcatgagc
     8521 acgcgcacga ccagcacggc gtggtagacg cggccgccct gggcggcgtg gaaggtgtcg
     8581 cgggacgact cgtcccggcc gacgtcgtac agctcaaggc tgagcttgcg cacggggctc
     8641 gaggtgagct cgtattgctt ctcgcttgcg atcgggtcct ctttgacggc cgggacggag
     8701 gggttgccgc cggtgacttt gcggtggaag gtgaaaacga aggaggggcg gtcgaggcgg
     8761 gccacgaagg tcttgtccgg aagcagggcg cgcagcggcg atcccacccg gcggctctcc
     8821 acgttgatct tgcgcatcgg gtaccagagg taccgcaacc ggggggcgca gttccagtac
     8881 atgagcgagc cgttgttgaa cggcatggag taggggatgt agtgctgaat gaaattggcg
     8941 ttggcgccgg cgttcgcgcc cgaatgcggc gtgaactggc ggcggatgta ctcatcgggc
     9001 tggcccgtgc cgtggcccag ttcgtgcgcc atcgtattcg gcacccaggt gtcgccgtgc
     9061 tcggtgacgc tgatgccgtt caggatggtg ggcgtatcct ggtagcagga cttgttcagt
     9121 ttgagcagcg agtaggggcg gttggcggag tcgcgaatgg cccattggta ggccgggccc
     9181 cagtcgccgt tttcgttgcg gcagacgagg gcgaggaatt tcgccttgcc gcccagggca
     9241 tcgcgctcgg cgtcgcgcag cgcctgctgg gcgaagaggg tctcgaagtt gtcggcggcg
     9301 ttatccgacg gttggttgtc gaaattgcag ttcacccacc cgcccgccgg cgctgtcggc
     9361 ttgaaatgct cccgctcgtc gaagaaatag aaatgccgga taatgaccgt gcgcgcggaa
     9421 ggcgcgattt cctccatgaa gagctgtttc cggttccaca catccatggc ccgatagcat
     9481 ccctggttgc ggaagttgtc gacctcggcc tcggtgggga tcggtccgaa ctgagccggg
     9541 gcgcgggtat cggccatgaa gctcatcgac gcatacgaga tggtgaacga gatcgggtgc
     9601 tcgtcgtcca catgaagtgc gtggaagtag tgcaggtcga aatcgccggt ggaggcgaat
     9661 tcggtgcggg tttcctgaag cgcccaggtg cagtggtagt cgctgtcgtt gcgcacggcg
     9721 gcgcgcgcgc cgaccacgaa tccgttggcc ggccagttcg gcgagggctc ggtgcgccgg
     9781 cggcccacgg gatagaaccc gtcgcggaag aaaacgattt tgacgtcttc ggcgggctcg
     9841 gtgatgagat tccgggcgaa cgggatccgg gcggagttca ggtcggcgcc cggctggtac
     9901 agcttgagga atcccgtggc cgggtcgacc accagcatgg cctgttcgta gtgggcgagg
     9961 atcgcctggg cgatcgcggc ggcgccgggg ctgctcatct aggacgtgct gccctgctgc
    10021 ggatcgaagt agtaggtcac gtgctggcgc gggctcaggt agttgtagat ttcccgcggc
    10081 a
//
LOCUS       DAOOKS010000004         9104 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_8911_length_9104_cov_3.764725, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000004 DAOOKS010000000
VERSION     DAOOKS010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9104
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8911_length_9104_cov_3.764725"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2351)
                     /locus_tag="PK112_00125"
     CDS             complement(<1..2351)
                     /locus_tag="PK112_00125"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HPC10487.1"
                     /translation="MCAGSPVLNFDEFARDRCFGHTFSNCFDTACCVVGAQLCFKVRA
                     TDPGATDDFIRLGEGANSNFLYQISLSQLLAIHTAGADTVWNFGDTMTVCLDLGNLPY
                     AGPYNVSSMIAALQDGTLDFMMIDDTQIDYLELIVQRCCPSQQYCSISGAKFNDLDHD
                     GKQDPGEPGLPGWTINLFYQGGPLYASAVTDANGNYTFANIPCSTYTVSEVNQPGWVQ
                     TTLPTAYTVTTAPGLPAVNISFGNWMCGGGNSGVPDTCCVKPPAKMLAWYPLDEAAPA
                     TALDLAGHDNNGTQLGSFGFAPGMVGGAYEFGPNVPNLDIVRVYNDPFVTIGPGDFSA
                     DAWIHPRSFGANCAVFNQNVGNQPCVDPIFDNRQWFFGGSGLDGVCFFVEDDGQGTGT
                     AFLGLIMSSFPAPQTVFYSPPGTVQLNQWQHVAVTVSRTTGGGTLYLNGAPVATFTPL
                     TGPLYSTNSGPILDIGHGTFLNTGGCYFTNRYFDGFLDEIEIFNRALTPAEVQGLYEA
                     GATGKCKVRCRVPTVVFCKNQTTRPLAFTVCNDGAASAALNWSLAGMASGSGCNFNGA
                     GLVFTPASGLTAPLAPGACTTITVTVTRPAGFNPGAVACYSLSVLNPMNGSSSTCEGQ
                     IKAGFGKWCATIIGLPIDPIGIGLGGGSGTARVMVTNDDDPTGSLSYGLQVLDHDGAP
                     SQAVSLNGLQPGEPVLGNRMIPLGTTQEFEVQLTALLWPGPGPENLVLSIDEDGDGVL
                     DRTADVGVEVISFADCNGNGADDAQDILSGVSTDANGNGVPDE"
     gene            complement(2327..2752)
                     /locus_tag="PK112_00130"
     CDS             complement(2327..2752)
                     /locus_tag="PK112_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10488.1"
                     /translation="MKYDDKNCNGTFDAGDVPLPNWIIELNTIWWGYQYDTTDALGQY
                     AFYNLNAGYHVLNEIQQPGWVQTAPSSVNYQLFIPSFNAVINNLDFGNLDPQLCDTTM
                     ADTCMAGAKDDFLGGTPRFPRRRPTSSRSCWRCARGRRC"
     gene            complement(2780..3718)
                     /locus_tag="PK112_00135"
     CDS             complement(2780..3718)
                     /locus_tag="PK112_00135"
                     /inference="COORDINATES: protein motif:HMM:NF028519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HPC10489.1"
                     /translation="MGSILRTAVYRRFGAGVLAAVFLCCPVAPALAQTGSISGIAFED
                     IDCDGSRDAHEAALGSIKIYLRGQAGGQPPTLDSTTTNADGAYSFAGLSSGDYDLSCA
                     VPDGYVQRGPVSVDYRFAHGGSGTMGGMDFTFRAEAGCDMVPTYCPAGVADNFDPANG
                     SEPSSPSADLAAQMATCGSSLPFFDQPASGACFGHTFSGCWDPCDIVRGELRLGLRAS
                     AAGSQDDELLLGDWPSPGRIWGASLSHLLEIKTGGADPSWDPGDTMTLVLDLGRLPLA
                     DRGLTNVLAAVQDGDLDVFIGSETEIDYLTGKMDGG"
     gene            complement(4002..4730)
                     /locus_tag="PK112_00140"
     CDS             complement(4002..4730)
                     /locus_tag="PK112_00140"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPC10490.1"
                     /translation="MALLTDKVAVVTGASRGVGAATARLLAAEGARVVLCARRREGLE
                     QAAHTLDPDGSRCLIAPADVGTLDGMKGLVDAALGRFGRIDLFINNAGAGVRKPIEET
                     TEGEWDLMFAVNVKAVFRSLRELIPVMRRQGGGHIINVSSMASTAGQPTLGPYAASKA
                     ALNLLTESAGEELRNDNIKVSLVMPGSIATNFLARVAADRNPSPAAKPRLTPEQVAAM
                     IVAIAAQDAGVWTSRVHIRPLRTK"
     gene            complement(4858..5025)
                     /locus_tag="PK112_00145"
     CDS             complement(4858..5025)
                     /locus_tag="PK112_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10491.1"
                     /translation="MSTFEMVRQRIRWYHVAGLAVLVVVTAGLVWFWLERRRTAALRA
                     EDKTDLAGGAE"
     gene            complement(5634..7877)
                     /locus_tag="PK112_00150"
     CDS             complement(5634..7877)
                     /locus_tag="PK112_00150"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPC10492.1"
                     /translation="MRVITDTADGLLAAAAHYLEELLAARDFSGAVKYYEANRRQLET
                     AGSALAGRILRLVARAHLALASYPTALRLARMAQAIHSEHGDTLELAETFLTVAEVLR
                     DSGELKQAEKAFRDAESIFRRNDCAEGQSRALNLLAGLYFRQNDYRNSLTVLMEAIGL
                     AKRLDDRKKLAYMMGNIGRIQTFLGEFAEAKKHLEINLTLSEELGQTLEAARAALSLG
                     YVQMQEGDFAGAETTFQKAAAGLRRVESPRDQVILRTYLGELYYRSERAAEAAEMLES
                     ALAAAEAIGRETTLTGRVLRHLAEASLRVGHDRVAARYAARALAILAKTGEMVEQGAV
                     LKVQAILAERGGRIAEARELFVKALDTLHQSGVRFEKAEALVAAGQSAAFDPRKRLTC
                     LFRAEEYFRRGGMQRRLEEIDRLLAGTDRLRPGDAPGAAARTSEVEFLTANEQIKRFK
                     QQLSYVAKSDLPVLLTGETGVGKDHMARYFHAICRPDGPFVAVNCASIPETLLESELF
                     GYAKGAFTGADRDKEGLFVTANGGVLYLDEIGDMPPTLQAKLLGVLESRRVVPVGSTK
                     EVPLDIRLVVATNRDLQAMVERGLFRRDLYYRISGITFHLPPLRDRKEDIPLLLRHFL
                     QNCRRLAESPLPADVIHRFVEYAWPGNVRELNNKVKRLEVMAELAAEGDLSELAHAVF
                     GDPGRDQRSSLFEQVERFERQMLIEALLATGGNKSEAARILGIHEATVRTKLKRYGIS
                     LEGGALN"
     gene            complement(7890..8264)
                     /locus_tag="PK112_00155"
     CDS             complement(7890..8264)
                     /locus_tag="PK112_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10493.1"
                     /translation="MKRLLTVSVVLVLLLATAVSSVSARRNPIAVPYPRPSSEDHPWG
                     GEQNYFPDSRLSQPGTSSGVAPGIVGIETSLLWRLVMPLWFERLTGHTIVTLPQPSTD
                     GNAIVGENNPGATAPANNGRGN"
     gene            complement(8587..8787)
                     /locus_tag="PK112_00160"
     CDS             complement(8587..8787)
                     /locus_tag="PK112_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HPC10494.1"
                     /translation="MITPETRVDEMVERYPDAVRFLMSRGLPCVVCGEPFWGSLEELC
                     RQKGWPTVRLAELVDEFNRLHS"
     gene            complement(8978..9103)
                     /locus_tag="PK112_00165"
     CDS             complement(8978..9103)
                     /locus_tag="PK112_00165"
                     /inference="COORDINATES: protein motif:HMM:NF021916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HPC10495.1"
                     /translation="MLSLVQFGDFVSYYLAILNEVDPTPVKIIDALKKALAEAKK"
BASE COUNT         1581 a         3044 c         2874 g         1605 t
ORIGIN      
        1 cactcgtccg gaacgccgtt gccgttggcg tcggtggaga caccgctcag aatatcctga
       61 gcgtcatccg cgccgttgcc gttgcagtcg gcgaacgaga tcacttccac cccgacgtcg
      121 gctgtccgat ccagtactcc gtcgccgtcc tcatcaatgc tcagcacgag attttcggga
      181 cccgggcccg gccaaaggag ggccgtgagc tggacctcga attcctgcgt cgtcccgagc
      241 ggaatcatcc ggttgcccag gaccggttcg cccggctgca gaccgttgag gctgacggcc
      301 tgcgacggcg cgccgtcgtg atcgagcacc tggagaccgt aggagagcga tccggtcggg
      361 tcatcgtcgt tggtcaccat gacgcgggca gtgccgcttc ccccgccgag gccgatgccg
      421 atcgggtcga tcgggaggcc gatgatggtg gcgcaccatt tgccgaaccc ggccttgatc
      481 tgcccttcgc aggtcgagga cgagccgttc atcgggttga gcacgctcag gctgtagcag
      541 gccacggcgc cggggttgaa tccggccggc cgggtcaccg tcaccgtgat cgtcgtgcag
      601 gcgcccgggg caagcggcgc ggtgagcccg ctcgcgggcg tgaacaccag gccggcgccg
      661 ttgaaattgc agcccgaccc cgacgccatc ccggccagcg accagttcag agccgcgctc
      721 gcggcgccgt cgttgcacac cgtgaacgcc agcggccggg tggtctggtt cttgcagaag
      781 accacggtgg gcacgcggca gcgcactttg catttgcccg tggctcccgc ctcatataga
      841 ccctggacct cggccggagt gagcgcccgg ttgaaaatct cgatttcatc gaggaaaccg
      901 tcgaagtacc ggttcgtgaa atagcagccg ccggtgttca ggaacgtgcc gtgcccgata
      961 tccaggatcg gcccgctgtt ggtgctgtag agcggcccgg tgagcggcgt gaaggtcgcc
     1021 accggcgcgc cgttgaggta gagcgtcccg ccgccggttg tccgcgacac cgtcaccgcc
     1081 acgtgctgcc actggttgag ctgcacggtt ccgggcgggc tgtagaacac cgtctgcggc
     1141 gccggaaaac tgctcatgat gaggccgagg aaagccgtcc ccgtcccttg gccatcgtcc
     1201 tcgacaaaga agcagacgcc gtcgagaccc gagccgccga agaaccactg gcggttgtca
     1261 aagatggggt cgacgcaggg ttggttgccg acattctgat tgaacaccgc gcagttggcg
     1321 ccgaagctcc gcgggtggat ccaggcatcg gcgctgaaat cacccggccc gatcgttacg
     1381 aacggatcat tgtagacccg cacgatgtcg agattgggga cattcggccc gaactcatac
     1441 gccccgccga ccatgcccgg cgcgaaaccg aaactcccga gctgcgtgcc gttgttgtcg
     1501 tggccggcca ggtcgagcgc ggtcgccggc gccgcctcat cgagcgggta ccaggccagc
     1561 atcttcgccg gcggcttcac gcagcacgtg tcgggaacgc cgctgttgcc gcccccgcac
     1621 atccagttgc cgaaactgat attcaccgcc ggcaggcccg gcgccgtcgt caccgtgtag
     1681 gcggtcggca gtgtcgtctg cacccatccc ggctggttca cctcgctgac cgtgtaggtc
     1741 gagcagggga tgttggcgaa ggtgtagttg ccgttggcgt cggtgacggc ggacgcgtag
     1801 agcggcccgc cctggtagaa caggttgatc gtccagcccg gcagccccgg ttcgcccgga
     1861 tcctgcttgc cgtcgtgatc gagatcgttg aacttggcgc cgctgatgct gcagtactgc
     1921 tgcgacgggc agcaccgctg cacaatcagc tccaggtagt cgatctgcgt gtcgtcgatc
     1981 atcatgaagt cgagcgtgcc gtcctggagc gccgcgatca tgctcgagac gttgtaaggc
     2041 ccggcgtagg gcaggttgcc gagatcgagg cagaccgtca tggtgtcgcc gaaattccag
     2101 accgtgtcgg ccccggccgt gtgaatcgcc agcagttggc tcaggctgat ctggtagagg
     2161 aagttggaat tggctccctc gccgagccgg atgaagtcat cggtcgcccc cgggtcggtg
     2221 gcgcgcacct tgaaacacaa ctgcgccccg accacacagc aggccgtgtc gaaacagttg
     2281 ctgaaggtgt ggccgaaaca gcggtcgcgc gcaaactcgt cgaaattcag caccggcgac
     2341 cccgcgcaca tctccagcag gatcgactgg aggtagggcg ccggcgtggg aatctcgggg
     2401 tcccccccag gaaatcatcc ttcgccccgg ccatgcaggt atcggccatc gtcgtgtcgc
     2461 acaactgcgg gtcgaggttg ccgaagtcca ggttgttgat gaccgcgttg aaactcggga
     2521 taaacaactg gtagttgacg ctcgacggcg ccgtctgcac ccaccccggc tgctgaatct
     2581 cgttgagcac gtggtagccg gcgttcagat tgtagaaagc gtactgcccg agcgcatcgg
     2641 tggtgtcgta ctggtaaccc caccagatcg tgttgagctc gataatccaa ttgggaaggg
     2701 gaacgtcccc tgcgtcgaaa gtgccgttgc agttcttgtc gtcatacttc acaccgtgaa
     2761 gttcagcttc cggcaagggt cagccgccgt ccatctttcc cgtgaggtaa tcgatctccg
     2821 tctcgctgcc gatgaagaca tccaggtcgc catcctgcac ggcggcgagc acgttggtca
     2881 ggccgcggtc ggccagcggg agccgcccga gatcgagcac gagcgtcatc gtgtcgcccg
     2941 gatcccagct cggatcggcc ccgcccgtct tgatctccag caggtggctc agcgaggcgc
     3001 cccagatgcg tccgggcgag ggccagtccc ccaggaggag ttcgtcatcc tggctgccgg
     3061 ccgccgaggc ccgcaggccc aggcgcagct cgccgcgtac gatgtcgcac ggatcccagc
     3121 agccgctgaa ggtgtgcccg aaacacgccc ccgaggccgg ctgatcgaaa aacgggaggg
     3181 acgacccgca cgtcgccatc tgcgcggcca ggtcggcgct gggcgaggac ggttccgagc
     3241 cgttggccgg atcgaaattg tccgccaccc cggccgggca gtacgtcgga accatgtcgc
     3301 agcccgcctc cgcccggaac gtgaaatcca tcccgcccat ggtcccgctt ccgccatggg
     3361 cgaaacggta gtcgaccgac accggccccc gctgcacata cccgtccggg accgcgcacg
     3421 acaggtcata gtcgccgctg ctcaaccccg caaacgagta tgccccgtcg gcattcgtgg
     3481 tcgtcgagtc cagcgtcggc ggctgcccgc ccgcctggcc ccgcaggtaa atcttgatgc
     3541 tccccaaggc cgcctcgtgg gcgtcacggg atccgtcgca gtcgatgtcc tcgaaggcta
     3601 tcccggaaat cgaccccgtt tgcgccagtg ccggcgcgac cggacagcac aggaaaacgg
     3661 cggcaagcac tcccgcccca aaccgccgat acacggcggt ccgcaggata cttcccataa
     3721 agtctccttg cctcgctatg gtatagaacg ttgtagggta tggtcgccgg cggccggaac
     3781 cgccgccggc agcgttactg taggatcaac atagagtaaa acgatgtata ttgtcaaata
     3841 ttattttcgc cgtcccgcaa ctttgtcacg gctgcgcggc cgctcgcgac acagtctgtc
     3901 ccccggccct acgcactccc tacgcaggcg ccgatggtgc gggaattggc ggaatttacc
     3961 ggggcatgcc cgccccgtgg cgatggcgca gaggggattg cctacttggt gcgcagcggc
     4021 cgtatgtgga cccgcgaggt ccacacgccc gcatcctgcg cggcgattgc gacgatcatc
     4081 gccgccacct gctccggcgt caggcgcggc ttggcggcgg gcgagggatt gcgatcggcg
     4141 gcgacccgcg cgaggaaatt ggtcgcgatt gaccccggca tgacgagcga caccttgatg
     4201 ttgtcgttgc gcagctcttc cccggccgat tcggtgagca ggttgagcgc ggctttcgaa
     4261 gcggcgtagg ggcccagggt cggctgcccg gcggtggagg ccatggacga cacgttgatg
     4321 atgtgcccgc ccccttgccg ccgcatgacc gggatcagtt cgcgcaggga acggaagacc
     4381 gctttgacgt tgacggcgaa catgagatcc cattccccct cggtcgtttc ttcgatcggc
     4441 ttgcggactc ccgccccggc attgttgatg aacaggtcga ttcgcccgaa ccggccgagc
     4501 gccgcgtcga ccagtccctt cataccgtca agcgtcccga cgtccgccgg ggcgatgaga
     4561 cagcgcgagc cgtccggatc gagcgtgtgg gcggcctgct cgagcccctc ccgccgcctc
     4621 gcgcagagca cgacccgggc cccttcggct gccagaagcc gggcggtcgc cgccccgacc
     4681 cctcgggagg ccccggtgac gaccgccact ttgtccgtga gtaatgccat ggctacagat
     4741 cgacccgacg agcgcggttc acccggcgga gagtctccgt cgcctccggc aggcgcgcaa
     4801 gccggccgtt cagcccggga gataccgcgg acccgcggat gcccccggga agtgttctca
     4861 ctccgccccg ccggccagat ccgtcttatc ttcagcccgg agcgctgcgg tccggcggcg
     4921 ctccaaccag aaccagacca gccccgcggt caccaccacc agcaccgcga gcccggccac
     4981 gtgataccac cggatccgct gtctgaccat ttcaaatgta ctcactgcat acctccgttc
     5041 ctggcggtcc cgttgaaatc atcggccttc ccttcccaca tccaaataac gccgcccccg
     5101 aaaaaacgca atcccattac gcgcacaagc ggcgatatcc gcccagagct gaagcgcccg
     5161 ctctccggcg gttcgttctt aaagtgatcg gccaaaaaaa taccacccgt gctcccctca
     5221 agcacgggtg gcgatccgcc aaagagaaaa gataagcgta acaattactg caacgctcag
     5281 ggagttaaga tgacagttat tggaacagca agcttcgtgc cgcatggtgt cgcattgcct
     5341 gacgacgccg tagtcgtttg catcacagca tattagctct gtgctacggt tccctgttcc
     5401 acattcgttc gtggaatttc gtacgatcat acgagtccgg tcccgaaaaa aagcggtaag
     5461 cgcctgtcaa tcaaacgctt accgcaacca aggagtaact gtgtctgttc gtaagccgct
     5521 acgattccac ccgcccggtt ccggtaccga tgcctccaag cgccatctca cacgctcggt
     5581 cgcgacgatc acacggagcg gggcgaggaa atccggctcg tgtcggtttc agcctagttg
     5641 agcgcacctc cttccaggct gatgccgtag cgcttgagtt tcgtgcggac ggtggcctcg
     5701 tggattccga ggatgcgcgc cgcctccgac ttgttgccac cggtggccag cagggcctcg
     5761 atcagcatct gccgctcgaa ccgctcaacc tgctcgaaga gcgaggagcg ttgatcccga
     5821 ccggggtcgc cgaacacggc atgggccaat tcggagagat ctccctcggc ggccagttcc
     5881 gccatcacct cgagccgttt gactttgttg ttcagctcgc gcacgttgcc cggccaggca
     5941 tactcgacaa agcggtggat aacgtcggcc ggcagcgggc tctccgccag gcggcggcag
     6001 ttttggagga agtgccgcag caggagcggg atatcctctt tccggtcgcg cagcgggggg
     6061 aggtggaacg taatgccgct gatgcggtag tagagatcgc ggcggaacaa tccgcgctcc
     6121 accatcgcct ggagatcgcg gttggtcgcc acgacgagcc ggatgtcgag cggcacctct
     6181 ttcgtcgatc cgaccggcac cacccggcgg ctttccagaa cgccgagcag tttggcctgg
     6241 agagtcggcg gcatgtcccc gatctcgtcg aggtagagca cgccgccgtt ggcggtcacg
     6301 aaaagtcctt ctttgtcgcg gtcggctccg gtgaaagccc ccttggcgta tccgaacagc
     6361 tccgactcca gaagggtctc gggaatcgag gcgcaattga ccgccacaaa aggaccatcg
     6421 ggccggcaga tcgcgtggaa gtaccgggcc atgtggtcct tgccgacacc cgtctcgccg
     6481 gtcagcagga cggggagatc ggacttggcg acgtagctca gttgctgttt gaaccgcttg
     6541 atctgctcgt tggcggtcag gaattccacc tcggaggtcc tcgcggccgc gccgggggcg
     6601 tcgcccggcc gcaggcggtc cgtgccggcc agcaggcggt caatctcctc cagacgccgc
     6661 tgcatccccc cgcggcggaa atactcttcg gcgcggaaca ggcaggtcag gcgtttgcgc
     6721 gggtcgaaag cggccgactg gccggccgcc accagcgcct cggccttctc aaaccgaacg
     6781 cccgactggt ggagcgtgtc gagcgctttc acgaacagct cgcgggcttc ggcgatgcga
     6841 ccgccgcgtt cggcgaggat ggcctgcact ttcagcacgg ctccctgctc gaccatctcg
     6901 ccggttttgg ccagaatcgc cagcgcccgg gccgcatagc gcgcggcaac ccggtcgtgg
     6961 ccgacacgca gcgaggcctc ggccaggtgg cgcagcacgc ggccggtcag ggtcgtctcc
     7021 cgaccgatag cctcggcggc cgccagcgcg ctctcaagca tctcggccgc ttccgccgcc
     7081 cgctcgctcc ggtagtacag ctcgcccagg taggtgcgga ggattacctg atcgcgcggg
     7141 ctttcgaccc ggcgcaaacc ggccgccgct ttctggaacg tcgtctcggc cccggcaaag
     7201 tccccttcct gcatctgcac gtagccgagg gacagcgccg cccgcgccgc ctcgagagtc
     7261 tgtcccagtt cctcggacag ggtgaggttg atttcgagat gtttcttggc ctctgcgaat
     7321 tcgccgagga atgtctggat acggccgatg ttgcccatca tgtacgcaag tttcttgcgg
     7381 tcgtccaggc gcttggcgag gccgatggcc tccatcagca ccgtcagcga attgcgatag
     7441 tcgttttggc ggaagtagag gccggccagc aggttcagcg ccctgctctg cccttccgca
     7501 caatcgttgc gccgaaagat tgattcggcg tctcggaatg ctttctccgc ttgcttcagt
     7561 tcgccgctgt cccgaagaac ttcggccacc gtgaggaaag tctccgccaa ctcgagcgtg
     7621 tcgccgtgct cggagtggat ggcctgcgcc atgcgggcca ggcggagcgc cgtggggtac
     7681 gaagcgagcg caaggtgagc ccgggccacc aaccggagaa ttcgtcccgc gagcgcgctt
     7741 cccgccgtct caagctggcg gcgattcgcc tcgtagtact tgaccgcgcc cgaaaaatcc
     7801 cgcgcggcga gcaattcctc caggtagtga gccgctgccg caagcagtcc gtcggcagtg
     7861 tcggtaatca ctctcatacc ttcgttccct cagtttcctc gaccgttgtt tgccggggcc
     7921 gtcgcacccg ggttgttctc tcccacaatt gcgttgccgt cagtcgacgg ctgcggcagt
     7981 gtcacgatgg tgtggccggt cagccgttcg aaccagagcg gcatcaccag ccgccacaac
     8041 aggctggtct caatgccgac aattccgggc gccactcccg acgaagtgcc gggctgggac
     8101 aggcgagaat cagggaaata gttctgctcc ccgccccacg gatggtcctc gctggacggc
     8161 cgcggatacg ggacagcaat cgggttccgg cgagcactga cactgctgac ggcggtggcc
     8221 aacaacagca ccaggacaac cgaaacagtg agaagacgtt tcatgacaca ctccttggtt
     8281 tatagtgaca caagttttgc caaagacaca gggccggcgg acgagagagg ggattgtgca
     8341 gagacatgag catacctccg gtcaagtccc tgccccttct actataatgt ggagcgcacc
     8401 ggttgtcaat gggatttttg aactcacccg ccgcggagca gattccgcgc tgctttcctg
     8461 tcgtctccgg taggatgacg taccatgcga aaggcgcgcg ccggcgtggc cgccccgccc
     8521 gtcgtctgtt cgccggccgg gccccgcaac acagtctctg cgaccgccgc accggccgct
     8581 cccccttcac gagtgcagcc gattgaactc gtccacgagt tccgcaaggc ggaccgtcgg
     8641 ccaacccttc tgccggcaca actcctccag gcttccccag aacggctccc cgcacacgac
     8701 gcacggcagt ccccggctca tgagaaagcg caccgcgtcc ggatagcgct ccaccatttc
     8761 gtcgactctg gtttccggcg taatcatctt cctgctccgc tcgggaccga tcgcggtcgc
     8821 gccgtgcggc gcgccgcctc aaactgttaa acgcccggag cccgacagag tgccgtggaa
     8881 gacgcggggg agggtttggc gcgccgggcc gcgcgcggga gaaaaaaagg cggggccggt
     8941 ctggcggccc cgcccaattg ccgtcggtcc cccggcgcta ttttttggcc tccgcgagcg
     9001 ccttcttcag ggcgtcgatg atcttcaccg gcgtcgggtc gacctcgttg agaatcgcga
     9061 ggtaatagga gacgaagtcg ccgaactgga ccagcgagag catc
//
LOCUS       DAOOKS010000005         8146 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_10344_length_8146_cov_3.577679, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000005 DAOOKS010000000
VERSION     DAOOKS010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8146
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10344_length_8146_cov_3.577679"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="PK112_00170"
     CDS             complement(<1..523)
                     /locus_tag="PK112_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010522376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HPC10496.1"
                     /translation="MASHRMGTAVPALLLLLVLAAAVSAADMQVRVYLDGKEDAAGLH
                     PLGLDVVWQGKDFIEIIATADELRELEGLGLRTEIVHGDVAAFYRSRLDHARDMGGYK
                     TLAEIQAYMDEIAFFHDAIVFPKTVIGYTLEGRPMYAMKISDNPELDEPDEPEVLYTA
                     AIHAREVITPETVL"
     gene            complement(622..1794)
                     /locus_tag="PK112_00175"
     CDS             complement(622..1794)
                     /locus_tag="PK112_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HPC10497.1"
                     /translation="MEPILTREEIEQRERATLAPYAALAAESRGRRFPSRDHPLRTVF
                     QRDRDRVVHAAAFRRLEYKTQVFIPHERDHFRTRLTHTIEVAQIARTLARSLRLNEDL
                     AEAIALAHDLGHTPFGHSGEDVLKDLLAGFGGFNHNRQTLRIVDLLEQRYPEHPGLNL
                     TYEVREGIVKHETVWDHKVAEFDDHAHPTLEAALVDVADELAYNAHDIDDGLSAGMLA
                     FDDLCRQPVWEVFGLDGRGAFERLHEDQKRYALVRRLIDLTATDVIAHTAAQIARFRI
                     ASLADVRSAPEKLTGYSPDLRPAVTALKQFLFSDMYQRPRLLALAKRARTILEGIFDR
                     LMAEPERLPARFREMLQTEETEIVVTDYMAGMTDRYAEKVYRELTGKEPPDLPEML"
     gene            complement(1799..1948)
                     /locus_tag="PK112_00180"
     CDS             complement(1799..1948)
                     /locus_tag="PK112_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10498.1"
                     /translation="MGLASEPLTFGGGIDIMHRGARLTYAAGYHPVLGLTHALALALE
                     TGRCR"
     gene            complement(2057..2623)
                     /locus_tag="PK112_00185"
     CDS             complement(2057..2623)
                     /locus_tag="PK112_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10499.1"
                     /translation="MRRPGRAAIGAGLLLACAAGAAAFELPTPRGEGFGRTMLLADAS
                     AAALTKAPTAGIRTGEWRFELGGVRRFELSDLDQVFAAAAGRLGRAVLAAGVSQFGNT
                     ELYAETTVRLAAGWRVGAFGAGGSLAAKIVEFGGGYGRLHAAAVGLSATYAGKRVQAA
                     AAAENLNCPRLDAAAAPDHRRLDVYVEI"
     gene            complement(2620..3594)
                     /locus_tag="PK112_00190"
     CDS             complement(2620..3594)
                     /locus_tag="PK112_00190"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HPC10500.1"
                     /translation="MRKVRNSAAARSQGAGEIGAPAAAASAAPAQAEVLALAKAMLGA
                     FEGTLGFILAVGALLAGAAAPVQAIRLNEVLANPTAGYGSEWIELENETDTAVSLAGW
                     RIGDALRTAVIIDTGAGPAVSDAAVPPRGYAVLVQDSAAFFASAGRPEAPVIEPPSWP
                     VLNNTGDSIRLIAPGGALHDHFVYRAVFPDNRTWCRHPIDPSRWDRSADPGGTPGREN
                     VLPDSDAAGAAALTVTPRVFSPDGDGVDDSTVIGLEADEELDRRVEIYDREGRRVWEM
                     AIPAGQRQAQCVWWGRSRAGERLPIGIYIIYLEAIGGESARRTVVIAR"
     gene            complement(3724..5358)
                     /locus_tag="PK112_00195"
     CDS             complement(3724..5358)
                     /locus_tag="PK112_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10501.1"
                     /translation="MRAVTLTAAITIALAAAGGTAQELSTDVYPSEDELLEALRRGDI
                     DQERYDILREILREGIDQPSRYLFNEIPNLAGEERADTALPRLAAEQAEAFTAKRPPA
                     PRLAVRHRYAQEIERSGRGSYRTTVSGRLGPHLDGAVRLERTVSGRERVVGRMIRWRP
                     ERGVICEAAVGSLARRLGLGTVAGYRGKILGSSPRLDGESVRYPDYGGQNGGYVRALA
                     GRWQAEALVSHTRDGEFTLLSAAVAVARRCGKVRAASIAGINRLRSRVRGEPRSDVKY
                     AGLLEYAYGNGRIAAEAAGQAGVRSAFGAVVFEGRHRLTGAEIRYCGWHYDRDYVDLA
                     GGSAGGTIAARDSIPGPAFAFSSRRTGNTGATVRTEAALTARVALETGVAGSIRSSDT
                     LRGEVLAGLIAAASRALQVRADHLERLTRTRESLYDRDAVVRRTRAEVRYDRAALAVR
                     TYVGYTVRSGAADSWNWFARLRYRSARVGDLEIWANLGTISPARRQIDYAYGYVQLRQ
                     MIGEAVAIAVKIAQRYSRGADDPHRTTVALEAEAVL"
     gene            complement(5363..6775)
                     /locus_tag="PK112_00200"
     CDS             complement(5363..6775)
                     /locus_tag="PK112_00200"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPC10502.1"
                     /translation="MVNRTIILLAPETAVASGIAWERLGETVTELSELYRLVRERRVD
                     LVLVDENRADLKRPVARRIRRQNGLTEIWLLTHDREPAPAEVQFIDGRLPLSLGPDGL
                     AQKIAHIYHGKHLLERYDMVGRAPQMKIVAETIERIAPTDVSVLVVGQSGTGKELVAR
                     ALHTQSARKDRPYVAINCGALAEGVLESELFGHERGAFTGSVARREGLFARAEGGTIF
                     LDEIGETKPDTQVKLLRVLEDGTYYPVGSSTARHADVRVIAATNRDLTEAISERRFRE
                     DLYFRIGVVKIVLPPLLDRKQDIEALLQHFWRGKELDYTDPALDLLLKYDWSGNVRQL
                     KNFADRMRALKPRGTVEVEDVERFIDEQHVTAKHLPVTTGKTVEEAGQELIYRAILQL
                     GAEIRLLRDLITAHLPSEPPEETAEAAPAADGATMEEVEERMIRRTLRETGGNRKEAA
                     RRLGIGERTLYRKLKKYGLS"
     gene            complement(6794..>8146)
                     /locus_tag="PK112_00205"
     CDS             complement(6794..>8146)
                     /locus_tag="PK112_00205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - exoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VacB/RNase II family 3'-5' exoribonuclease"
                     /protein_id="HPC10503.1"
                     /translation="YLPGMVIPMLPESLSNDVCSLKPNRKRLTFSVFMDFDRAGKMLS
                     WEIRPSAIKSRAKLSYEEVQEFFDKGLPEGAGRRKLERVAENLTTARELARLLSARRF
                     AEGSLDFDLPEAKITLNEKGEVIELGNRVRLESHRLIEEFMLSANKAVALEMFRSAQA
                     FLYRVHDRPDMEKLEAFSAMVARLGYRFPVSPTTKPGDLARFLETVKDKPEAEFINEL
                     MLRSLKKAVYQRNNVGHFGLAFTHYTHFTSPIRRYPDLLVHRLLRMRRPDGKYSPAAA
                     RRIPAVIDHAGKHCSETERIAEAAEREAVKVKQVQYMARHVGDFFGGIISGVTGFGFF
                     VRLDNLGAEGLVRVSTIDDDYYRFDEQRYALIGRRTGRMFRLGDRVEVGVLSVDKVNN
                     EIALFAPGLPAQAKAGSGPKGKRKKKGWAEAQPPKAKTPARTRTAPPGYGRTRKKRPR
                     "
BASE COUNT         1190 a         2855 c         2712 g         1389 t
ORIGIN      
        1 gaaggaccgt ctcgggggtg atcacctcgc gggcgtgaat ggccgcggta tagagcacct
       61 ccggctcgtc gggttcgtcg agctccgggt tgtcggaaat cttcatggcg tacatcgggc
      121 gtccctcgag cgtgtatccg atcacggttt tcgggaagac gatcgcgtcg tggaagaagg
      181 cgatttcatc catgtacgcc tggatttccg cgagcgtctt gtaccctccc atgtcgcgcg
      241 cgtggtcgag acgcgagcgg tagaaggcgg cgacgtcgcc gtggactatt tccgtgcgca
      301 gccccaaacc ctcgagttcg cggagctcgt cggccgtcgc gatgatctcg ataaagtcct
      361 ttccctgcca gaccacgtcg agccccaggg ggtgaagccc ggcggcgtcc tccttgccgt
      421 cgagatagac gcgcacctgc atatcggcgg cggatacggc ggcggcgagc acgagcagaa
      481 gcagcaacgc ggggacggcc gtccccatgc ggtgagaagc catgttgtcc tccgaaccag
      541 gcggaaactt cgattgcccg taatatacac cggaaggccg attttaccaa tgaataatcg
      601 gctgcgggag cggggcggca actagagcat ttcggggaga tccggcggtt ctttccccgt
      661 gagctcccgg tacactttct cggcgtagcg gtccgtcatc ccggccatgt agtcggtgac
      721 gacaatttcc gtttcttcgg tctggagcat ctcccggaac cgcgccggca aacgttcggg
      781 ttcggccatg aggcggtcga agattccctc gagaatggtg cgcgcccgtt tcgccagcgc
      841 caggaggcgc ggtctctggt acatgtcgga gaagaggaac tgcttgagcg cggtgaccgc
      901 cgggcgcaga tccggggagt agcccgtcag cttctccggc gcgctgcgca cgtcggcgag
      961 actcgcgatc cggaagcgcg cgatctgcgc ggcggtgtgg gcgatcacat cggtggccgt
     1021 gaggtcgatc agccgccgca cgagcgcgta gcgtttctgg tcctcatgga gccgctcgaa
     1081 cgccccgcgc ccgtccagcc cgaacacttc ccagaccggc tggcggcaca ggtcgtcgaa
     1141 ggcgagcatc cccgccgaca gaccgtcgtc gatgtcgtgg gcgttgtacg ccagttcatc
     1201 ggcgacgtcc acgagcgccg cctcgagcgt cgggtgcgcg tggtcgtcga actccgccac
     1261 cttgtggtcc cacaccgtct cgtgtttgac aatcccctcg cgtacttcat aggtgaggtt
     1321 gagccccggg tgctcgggat agcgctgctc gagcaggtcg acgatccgca gggtctggcg
     1381 attgtggttg aacccgccga acccggcgag cagatccttc agcacgtcct ccccggaatg
     1441 tccgaagggg gtgtggccga gatcgtgggc cagggcgatg gcttcggcca gatcctcgtt
     1501 gaggcggagg ctgcgggcga gcgtccgcgc gatctgcgcc acctcgatcg tgtgcgtaag
     1561 ccgggtgcgg aaatggtccc gctcgtgggg gatgaacacc tgcgtcttgt attcgaggcg
     1621 gcggaaggcg gcggcgtgga caacccgatc acggtcgcgc tggaaaaccg tccgcagggg
     1681 gtggtcgcgc gagggaaagc gccgtccccg cgattcggcc gcgagcgccg catacggcgc
     1741 cagggttgcc cgctcccgct gctcgatctc ctcccgggtc agtatcggtt ccatccgttc
     1801 acctgcatcg gccggtttcc agggcgagcg ccagcgcgtg cgtcaggcca agcaccgggt
     1861 ggtacccggc ggcatacgtg aggcgggcgc cgcgatgcat gatatcgatt cccccgccga
     1921 aggtcaacgg ctcagaggcc aggcccacga atattgcggc cggcggcgcc accgcgaacc
     1981 gctggccgat gccgaaccgg ggggactgcg tcggctcaaa actcgcccgc ccggtcgtct
     2041 ggtaggcgcc gttcccctag atttccacgt acacatccag ccggcgatgg tcgggcgcgg
     2101 cggcggcgtc caggcgcggg cagttaagat tctcggccgc ggccgccgcc tgcacgcgct
     2161 tcccggcata ggttgccgag aggccgaccg ccgcggcgtg gaggcggccg tacccgccgc
     2221 cgaattcgac gatcttcgcg gcgaggctgc ccccggcgcc gaatgcgccc acccgccagc
     2281 cggccgccag acggaccgtc gtctcggcat acaactccgt attgccgaac tgggacacgc
     2341 ccgccgccag caccgcccgg cccagtcgtc cggccgcggc ggcgaacacc tggtcgaggt
     2401 cgctcagttc aaagcgccgc acgccgccga gttcgaaccg ccactccccc gtgcggatcc
     2461 cggcggtcgg cgccttggtc agcgcggcgg ccgaggcgtc ggccaggagc atggtccggc
     2521 cgaagccttc gccgcgcggc gtcggcaatt cgaacgccgc ggcgccggcg gcgcaggcca
     2581 aaagcagacc cgcgccgatc gccgcccgcc ccggccgtct catcgcgcga tcaccacggt
     2641 ccggcgcgcc gactccccgc cgatcgcttc gaggtaaatg atgtagatcc cgatcgggag
     2701 acgctcgccc gctcgcgacc gcccccacca cacgcactgc gcctgccgct gccccgccgg
     2761 gatcgccatc tcccacaccc gtcgtccctc gcgatcgtaa atctccaccc ggcggtcgag
     2821 ctcctcgtcc gcctccagcc caatcacggt ggagtcgtcg acgccgtctc cgtccggcga
     2881 gaacaccctg ggcgtcacgg tcagcgccgc cgcgccggcg gcatcagagt cgggcaggac
     2941 gttctctcgg ccgggcgtgc ctcccggatc ggccgaccgg tcccaccgcg aggggtcgat
     3001 cgggtggcgg caccaggtcc ggttgtccgg aaacacggcc cgatacacga agtgatcgtg
     3061 gagcgcgccc cccggggcga tcaggcggat gctgtcgccg gtgttgttga gcacgggcca
     3121 cgagggcggc tcgatcaccg gcgcttccgg ccggccggcg gaggcgaaga atgcggcgga
     3181 atcctgcacg aggacggcat atccccgggg cgggacagcc gcgtctgaaa cagccgggcc
     3241 cgctccggtg tcgataatca ccgctgtccg cagcgcgtct ccgatccgcc atccggcgag
     3301 actgacggcg gtatcggttt cgttctccag ttcgatccac tccgatccat aacctgcggt
     3361 cggattggcg agcacttcgt taaggcggat cgcctgcacc ggcgccgccg caccggccag
     3421 gagggcaccg accgccagga tgaaaccgag cgtcccttcg aacgccccca gcatggcctt
     3481 cgccagcgcg aggacctccg cctgggctgg cgccgccgag gccgcggcgg ccggcgcgcc
     3541 tatctcgccg gcaccctgag accgcgccgc ggccgaattc ctgaccttcc gcatcgtacc
     3601 tcctcccctg tatccgggtg ttcgtgcagg tccggaccgt tatggtcgtc aattgtttct
     3661 tttcgctgcc ggcgtcaaaa ccatccccca tttcatgcca gatccatgcg tcacctcgcg
     3721 tcgctacagg actgcttcgg cctcgagcgc caccgtcgtg cggtgagggt cgtccgctcc
     3781 ccggctatag cgctgcgcga ttttcaccgc gatcgccacc gcctccccga tcatttgccg
     3841 caactggaca tacccgtagg cgtagtcaat ttgtctgcgc gcgggcgaga tcgtccccag
     3901 gttcgcccag atttcgagat ccccgacccg cgcagagcgg tagcggagac gcgcgaacca
     3961 gttccaggag tcggcggcgc cgctgcggac ggtgtacccg acataggtgc gcacggccag
     4021 ggccgcgcga tcgtaccgca cctcggcgcg cgtgcggcgc acgacggcgt cgcggtcgta
     4081 caggctctcc ctcgtgcggg tgaggcgctc caggtgatcg gcccggacct gcagcgcgcg
     4141 gctcgcggcg gcgataagcc ccgccaggac ctcgccgcgc agcgtatcgc tgctgcggat
     4201 ggatccggcc acgccggtct cgagcgccac ccgcgccgtc agggcggcct cggtgcgcac
     4261 cgtggcgccg gtgttgccgg tgcgccgcga ggagaacgcg aacgccggac cgggaatgct
     4321 gtctcgcgcg gcgatcgtcc cccccgcgct gccgcccgcc agatcgacat aatcgcgatc
     4381 gtagtgccac ccgcagtagc ggatctcggc ccccgtcagg cggtgccgtc cttcgaaaac
     4441 gacggcgccg aaggcgctgc gcacgcccgc ctgcccggcc gcttccgcgg cgatccggcc
     4501 attgccgtag gcatattcga gcaaccctgc gtacttgaca tcgctccgcg gctcgccgcg
     4561 cacgcggctg cgcaggcggt tgatccctgc aatcgatgcg gcccgcactt tgccgcaccg
     4621 gcgggcgacc gccaccgcgg ccgacagcag ggtgaactcc ccgtcgcggg tgtgcgagac
     4681 gagcgcttcg gcctgccacc gccccgcgag cgcgcggaca tatccgccgt tctgcccgcc
     4741 gtagtcgggg taccggaccg actcgccgtc gagccgcggc gaactcccga ggatctttcc
     4801 acggtagccg gccaccgttc cgagccccag ccggcgggcg agggatccga ccgcggcctc
     4861 gcaaatcacg ccccgctcgg gccgccagcg gatcatcctt cccaccaccc gttcgcgccc
     4921 ggaaaccgtc cgctccagcc tcaccgcgcc gtcgagatgc gggccgagcc gcccggagac
     4981 agtggtccgg tagcttcctc ttccgctgcg ctcgatctcc tgcgcgtacc gatgcctgac
     5041 cgccagccgc ggcgccggcg gccgcttggc ggtgaaggct tcggcctgct ccgccgccag
     5101 gcgcgggagc gccgtgtcgg cgcgttcctc cccggccaga ttggggattt cgttgaacag
     5161 gtagcgggac ggctggtcga ttccctcgcg caggatttcg cggagaatgt cgtaccgctc
     5221 ctgatcgatg tcgccccgcc gcagcgcctc gagcaactcg tcctcggagg gatagacgtc
     5281 ggtcgagagt tcctgggcgg tccctcccgc ggccgcgagt gcgatggtga ttgcggcggt
     5341 cagcgtgacc gctcgcatgg tgtcagctca gcccgtactt cttgagtttc cggtagagcg
     5401 tgcgctcgcc gatccccagc cgccgcgcgg cttccttgcg gttgccgccg gtttcgcgca
     5461 gggtccgccg gatcatgcgt tcttccacct cttccatggt cgcgccgtcg gccgccggcg
     5521 cggcctcggc cgtctcctcg ggcggttcgc tcggcaggtg ggcggtgatg aggtcgcgca
     5581 ggagtcgaat ttcggcgccc aactggagaa tcgcccggta gattaactcc tgtcccgcct
     5641 cctcgactgt cttgccggtc gtgaccggca ggtgttttgc cgtgacgtgt tgctcgtcga
     5701 tgaaccgttc gacatcctcg acctccaccg tcccgcgcgg tttcagcgcc cgcatgcgat
     5761 cggcgaaatt cttcagttgc cgaacgttgc ccgaccagtc gtatttcaac agaagatcga
     5821 gcgccgggtc ggtgtagtca agctcttttc cgcgccagaa atgctgcagg agcgcctcga
     5881 tgtcctgttt gcggtccaaa agcggcggca ggacgatttt cacaacgccg atgcggaagt
     5941 acaggtcctc gcggaagcgc ctttcgctga tggcctcagt gaggtcgcgg ttggtggccg
     6001 cgatcacgcg cacgtcggca tgccgcgccg tcgacgatcc caccgggtag taggtgccgt
     6061 cctcgagcac gcgcagcagt ttgacctggg tgtcgggctt ggtttcgccg atttcgtcga
     6121 ggaagatcgt ccccccttcg gcccgcgcga acagtccctc gcgtctggcg accgacccgg
     6181 tgaacgctcc gcgctcgtgc ccgaacagct ccgattcgag cacgccctcg gcgagcgcgc
     6241 cgcagttgat ggccacgtac ggccggtcct tgcgcgccga ctgcgtgtgc agggcccggg
     6301 ccacgagttc cttcccggtg ccggactgcc cgacgacgag gaccgagacg tcggtcggcg
     6361 cgatccgctc gatcgtttcc gcaacgatct tcatctgcgg cgcgcgcccc accatatcgt
     6421 accgttcgag caggtgcttg ccgtggtaga tgtgggcgat tttttgcgcg agcccgtccg
     6481 gcccgagcga cagcggaagg cgcccgtcga taaactgcac ctcggccggg gccggctccc
     6541 ggtcgtgggt gagcagccag atttcggtca ggccgttctg gcggcggatt ctgcgggcca
     6601 ccggccgttt gaggtcggcc cgattctcat cgaccaggac cagatccacg cgccgctcgc
     6661 gcacgaggcg gtagagctcc gacagctcgg tgaccgtttc gccgaggcgc tcccaggcga
     6721 tcccggaggc gacggcggtt tcgggcgcga ggagtatgat cgtgcggttt accatggcgg
     6781 tcgattcccc gtctcagcgc gggcgcttct tgcgggtgcg cccgtacccc ggcggggcgg
     6841 tcctggtccg cgcgggggtc ttggccttgg gcggctgcgc ctccgcccac cctttcttct
     6901 tgcgcttccc cttcggcccg ctcccggctt tcgcttgagc gggcagcccc ggggcaaaga
     6961 gcgcgatttc gttattcacc ttgtccaccg agagcacccc cacctccacc cggtcgccca
     7021 gacggaacat ccgccccgtc cgccgcccga tgagggcgta gcgctgctcg tcgaaccggt
     7081 agtagtcgtc gtcaatcgtc gatacccgca ccagcccctc ggcgccgagg ttgtccaggc
     7141 ggacgaagaa gccgaatccg gtgactccgg agatgatgcc cccgaagaag tcgccgacgt
     7201 gccgcgccat gtactggacc tgcttcacct tcaccgcctc ccgctccgcg gcctcggcga
     7261 tgcgctcggt ctccgagcag tgttttccgg cgtggtcgat gactgcagga atccgtcggg
     7321 ccgccgccgg cgagtacttc ccgtcgggcc gccgcatccg cagcagccgg tggacgagca
     7381 ggtcggggta gcggcggatg ggcgaggtaa agtgggtgta gtgggtgaac gccagcccga
     7441 agtggccgac attgttgcgc tggtagaccg ccttcttcag cgagcggagc atcagctcgt
     7501 tgatgaattc ggcctccggc ttgtccttca ccgtttccag gaagcgggcg aggtctcccg
     7561 gtttggtcgt gggggagacg gggaagcggt atccgaggcg ggccaccatg gccgagaagg
     7621 cctcgagttt ttccatgtcg gggcggtcgt ggacgcggta gaggaaggcc tgggcactgc
     7681 ggaacatctc gagcgcgacc gccttgttgg ccgacagcat gaactcctcg atcagccggt
     7741 gcgattccag gcgcacgcgg ttgcccagct cgatcacctc ccccttttcg ttgagggtga
     7801 tcttggcttc gggcaggtcg aaatcgaggg atccctcggc gaaccggcga gcgctgagca
     7861 ggcgggcgag ctcgcgggcc gtcgtgagat tctcagccac ccgctcgagc ttccgccgcc
     7921 ctgcgccctc ggggagccct ttgtcgaaaa actcctgcac ctcctcgtag ctcagcttgg
     7981 cccgcgactt gatcgccgac ggccggatct cccacgacag catcttcccc gcccggtcga
     8041 aatccatgaa caccgagaag gtcaggcgct tccggttcgg cttgagcgaa cagacgtcgt
     8101 tcgagaggct ctccggcagc atcgggatca ccatcccggg caggta
//
LOCUS       DAOOKS010000006         7857 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_10873_length_7857_cov_3.840810, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000006 DAOOKS010000000
VERSION     DAOOKS010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7857
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10873_length_7857_cov_3.840810"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="PK112_00210"
     CDS             complement(<1..366)
                     /locus_tag="PK112_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPC10504.1"
                     /translation="MTSTAPAAGAAGGAANPGLFRQLASLPRPFWMANVIEMFERLAY
                     YGVRVVIPIYIAQADEPGGLHFTQADKGLIFMIWAIVQSLVPMVSGGFADRYGYKKTM
                     AFAFTVKIIRYLTMATQREF"
     gene            496..780
                     /locus_tag="PK112_00215"
     CDS             496..780
                     /locus_tag="PK112_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012099192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HPC10505.1"
                     /translation="MTDHAPDPVEYPIDGTLDLHQFAPEDTRAVVEEYLRACLEKRIY
                     ALRIVHGKGSGVKRRIVHALLARHPNVVRFRHEEGSGGSWGATVVDLKPD"
     gene            794..1309
                     /locus_tag="PK112_00220"
     CDS             794..1309
                     /locus_tag="PK112_00220"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HPC10506.1"
                     /translation="MEPYLREILWRQCGAAIDMLENAIAACPDEVWAAPDGPAKAGDC
                     WYLAYHTLFWLDLYLDGAAEGFAPPAPFGLEELDPAGRLPERAYTQDQLLAYLAHGRE
                     KCRAAVAGLTDQSARRPCVFRWGEVAYVELLLYTMRHVQHHAAQMNRLLREATGSAPH
                     WVGITRRPLSR"
     gene            complement(1426..7824)
                     /locus_tag="PK112_00225"
     CDS             complement(1426..7824)
                     /locus_tag="PK112_00225"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPC10507.1"
                     /translation="MADSCGDADTVSHTYNITINQPPTIALGADTALALCAPQPICLS
                     YAAGDPDAGDLVTETMVSGYGAIDTAANQICFTPTSGGLYQFIVQATDNCGDSDLDTA
                     LVLVSFGDVAAIDCPTGPINVSLCAADEVCQMLAVTPPTAAVTASFGTWAAGQLCFQA
                     DTSGTYHIQVVASEDCGADTCLLTFNVVIGAAASITCPEPQTESLCAADTVCVPVSIN
                     GGNLSVTVTPTGWYSAGNVCFVADTSGHYALTVIANTLCGADTCAFTVDVTINTPPTA
                     ADPSSPVDTFMCAAAQVCYQFAAADAEGDPLTWSRLSGSGTVSSSGLWCFTAAAGSHS
                     VTAVAADPCGAKDTVSLTYEVAINSAPVMALGNDTTVFQCAATAMCLPYAATDADGNI
                     TLIEILAGAPGAVLDQPNSRFCFTPAGAGSYTFILRVTDACGQSDRDTMVVTVGLNSS
                     PAVSLGADQTLFACAPAQICLPVASSDPDNNLSAVTLVSGPGTLVGNQVCFTPTGTGT
                     YQFIVRATDACGAVDEDTALVYYTLNSAPTADAGADQTLFLCAPAPICWPAAGSDPDG
                     NLASVTLTQGPGTFNGSQICFTPSADGSYLFVLRATDACGAADYDSATITVALNEAPV
                     CVAPSDTSIAQCAAQEVCLPAYATDANGNLHMCQIISGPGSLVNGQWCYTPASDQTVT
                     VVMRCTDSCGAYCESQFTVDFDVNGPPQIAFGADINTFQCQLAEICLPYTASDPEGLG
                     TTTITLVSGPGTLDQANSRVCYTPAAPGTATFIIGIADHCNRADYDTINVTVGLNVPP
                     AVNAGSDQDLFLCQTGAEICWPASVTDANNNLTSVNLIGPGTFDGDEICFNPTTSGDY
                     DFILEATDACGVYRADTVTITVTNNEPPTVQLPGDSSLFLCAPQEVCFDYAADDPKGG
                     RLTETMLSGFGTIDTAANRICFTPAAAGSYEFVVRVTDSCGASDRDTMVVTVAFGTFV
                     SIDCPSTPIAVSLCGPDEVCYLLGITPGSATVSTSHGTYSGGQLCFPADTTGTYAIEV
                     IASTDCNADTCILEFTVDIGAAAQISCPGPQTFDICTPDTVCVPVTVLMRSDTVTVSP
                     IGWYAGGNVCFFADTSGHYELTVIAATTCGADTCTVIADVSINTAPAADLPDPVDSFL
                     CASGQICFAFAAADADGDPLTWTRLSGPGTVTPAGVWCFTPTEGGAYPVTVIVADPCG
                     AADTATMTYNLTFNTPPVVDLGGGKVNKLTLFQCDPSEQCLPYTVTDPDNNVVREELV
                     SGTATLDTAANELCFTAPASGTYRFIVRVEDACGAFDLDTLDVTVQINYAPTANAGAD
                     QQLFLCASTPLCWPAGCTDPDNNLDSCYLITPAGTYSGGQICFTPDTAGIYGFILRTV
                     DVCGEADEDTAFVAVEINSAPVCHLPNDTTIAQCLPAQVQLPVSATDVDGNLDRCEII
                     TGPGSIQSGAWVYTPTADQTVTVQVLCIDSCGTVCTGDFTVAFQVNEPPAVNLGPDTT
                     LFLCQPQQVCRPVAVTDDENNLQSTTVISPAGATLNGDQVCFATALGQPTYPVIVEAV
                     DACGRSRRDTSIVTVNFNRPPALTVTPSFTIYLDFSGEVCFSVAATDPDNNLTGVGVT
                     APAALAGNEACFWADTSGVYCFVVTAHDACNVAAVDTICVTVAIDECIHLRVEKTHNS
                     FQGNHEFVDIFQQGSGKEIGGFDLLLQYDPTALLFNNAVPGDLLEQCGWEYFTYRQGP
                     AGNCSGCPNGLVRIVAIAEYNNGANHPGCFLQDLTGSLATLDFVVSNDRNLECQYAPI
                     RFFWVDCGDNAVSSRTGDTLFISRKVFDFELNEMTSYTGTLPGFTGAPDVCITSGGPG
                     KPSTVRCVDFTNGGVDIVCADSIDARGDINLDGQMNTIADAVLLSNYFVYGLGVFTIN
                     VEGQIAATDVNADGLALSVADLVYLIRVVVGDALPYPKLDPNKSAEVQLALIDGVLEI
                     QRTDYEIGAIALILEGEAVPELHPEAKGMELRYSFDGTDTRVLVYNSQAAAALSGGKV
                     LRIGGAARIKTIDLGAFNGLALTAKVSSLPSRYALSQNFPNPFNPATTIEFALPKTTD
                     WELKIFNVLGQTVETFTGTQDVGYMKIVWDAGRYASGVYLYRLTAGEFTATKKMVLLK
                     "
BASE COUNT         1285 a         2661 c         2605 g         1306 t
ORIGIN      
        1 gaactcccgc tgcgtggcca tcgtgaggta cctgataatc ttgaccgtga acgcgaaggc
       61 catcgtcttc ttgtagccgt agcggtcggc gaagcccccc gagaccatcg gcaccagcga
      121 ctgcacgatc gcccagatca tgaagatgag tcccttgtcg gcctgggtga aatgcagccc
      181 gcccggctcg tcggcctggg cgatgtaaat gggaatcacg acccgcactc cgtagtaggc
      241 caggcgctcg aacatctcga tgacgttggc catccagaag gggcgcggaa gggaagcgag
      301 ttggcggaaa aggccggggt tggcggcccc gcccgcggca ccggctgccg gcgcggttga
      361 cgtcatggta cctcctgatg tatattttcc agaccgctgc gccaattaac atgccggcgg
      421 ggcgcaaggc aattgattca tgcgccccgg cagcttccgc gccgcggccg caacccgcca
      481 ccgggagaag aacctgtgac cgaccacgcc cccgacccgg tcgagtaccc gatcgacggc
      541 accctcgacc tccatcaatt cgcccccgag gacacccgcg cggtagtcga ggagtacctt
      601 cgcgcctgcc tggaaaagcg gatctacgct ctgcggattg tgcacggcaa ggggagcggg
      661 gtcaaacgcc gcatcgtcca cgccctcctc gcccgccacc ccaacgttgt ccgcttccgc
      721 cacgaggagg gttccggggg atcatggggc gccacggtgg tcgacctgaa gccggattga
      781 caaggtggag gctgtggaac cgtatctgag ggagattctc tggcgccagt gcggagccgc
      841 cattgacatg ctcgagaatg ccatcgccgc ctgcccggac gaggtctggg cggcgccgga
      901 cgggcccgcc aaggccgggg actgctggta cctcgcctac cacaccctgt tctggcttga
      961 tctctatctc gatggtgcgg cggaggggtt cgcgccgccg gccccgttcg ggctcgagga
     1021 gctcgatccg gctggacggc ttccggagcg ggcctacacg caagaccaac tgctggccta
     1081 cctggcgcac ggccgcgaga agtgccgcgc cgccgtcgcc gggctgaccg atcaatcggc
     1141 gcgccgcccc tgcgtctttc gctggggcga ggtcgcatac gtggaactgc tgctgtacac
     1201 gatgcgccac gtgcagcacc acgcggccca aatgaaccgc ctcctgcgcg aggcaaccgg
     1261 ctcggctccg cactgggtcg ggatcacccg gcggccgctc agccggtgag cgccgcctgc
     1321 gtcccggccg ccgccaatga caaaccccgc gggcagtagg cgcggggttt gtcgtttatg
     1381 aagggtcccc ctctcgggtt accctccggg tcggtggccc tgccgctact tcagcagaac
     1441 catcttcttc gtggcggtga actcaccagc cgtcagacga tagagataca cccctgaggc
     1501 gtatcgtccg gcatcccaca caatcttcat gtacccgacg tcctgggtcc ccgtgaacgt
     1561 ctcgaccgtc tggccgagca cgttgaagat cttgagttcc caatcggtcg tcttgggcag
     1621 ggcgaattcg attgtcgtcg ccgggttgaa cgggttcggg aagttctgcg agagcgcgta
     1681 acgcgacggc aggctgctca ccttggccgt cagcgcgagg ccgttgaagg ccccgaggtc
     1741 gatcgtctta atcctcgccg cccccccgat ccgcaggacc ttcccgccgg agagcgcggc
     1801 cgccgcctgg ctgttgtaga cgagcacgcg ggtgtcggtc ccgtcgaagc tgtaccgcaa
     1861 ctccatcccc tttgcctccg ggtgcagttc cggcacggcc tcgccttcga ggatcagggc
     1921 gatggcgccg atctcgtagt cggtcctctg gatctccagc acgccgtcaa tcagcgccaa
     1981 ctgcacctca gccgacttgt tgggatcgag tttcggatac gggagcgcgt cgccgaccac
     2041 tacccggata aggtagacca aatcggccac cgacagcgcc agcccgtcgg cgttcacgtc
     2101 cgtggccgcg atctgcccct ccacgttgat cgtgaacacg cccaggccgt agacgaaata
     2161 gttgctcagg agcacggcgt cggcgatcgt attcatctgg ccgtcgaggt tgatgtcgcc
     2221 gcgggcgtcg atcgagtcgg cgcacacgat gtccacgccg ccgttggtga agtccacgca
     2281 gcgcacggtc gagggcttgc cgggaccgcc gctcgtgatg cagacgtccg gcgcccccgt
     2341 gaaccccggc agcgtcccgg tgtacgaggt catctcgttg agctcgaaat cgaacacctt
     2401 gcgcgagata aagagcgtgt cgccggtgcg cgacgacacc gcgttgtcgc cgcaatccac
     2461 ccagaagaac cggatcggcg catactggca ctcgaggttg cggtcgttgg acacgacgaa
     2521 gtcgagcgtc gccagcgaac cggtcagatc ctgcaggaaa cagccggggt ggttggcgcc
     2581 gttgttgtac tcggcgatcg ccacaatccg caccaggccg ttgggacagc cggagcagtt
     2641 gcccgccgga ccctggcggt aggtgaagta ctcccagccg cactgctcga gcaggtctcc
     2701 gggcacggcg ttgttgaaca ggagagccgt gggatcatac tgcagcagca ggtcgaaccc
     2761 accgatctcc ttgcccgatc cctgctggaa gatgtcgacg aactcgtggt tgccctggaa
     2821 gctgttgtgg gtcttctcca cccggaggtg gatgcactcg tcgatcgcca ccgtcacgca
     2881 gattgtgtcg acggcggcaa cgttgcaggc atcgtgcgcc gtcacgacaa agcagtacac
     2941 gcccgaagta tcagcccaga agcaggcttc gttccccgcc agcgccgccg gcgccgtcac
     3001 cccgaccccg gtgagattgt tgtccgggtc ggtcgccgcg acgctgaagc acacttcgcc
     3061 cgagaagtcg aggtagatcg tgaaactcgg cgtcacggtg agcgccggcg ggcggttgaa
     3121 gttgaccgtg acaatgctgg tgtcgcgccg cgagcggccg caggcatcga ccgcctcgac
     3181 aatgaccggg taagtcggct ggccgagcgc ggtcgcaaaa cagacctggt cgccgttgag
     3241 cgtcgccccg gccggcgaaa tgaccgtcgt gctctgcagg ttgttctcgt catcggtcac
     3301 cgccaccggg cggcacacct gctggggctg gcagaggaac agcgtcgtgt cgggtccgag
     3361 gttcaccgcc ggcggctcgt tcacctggaa agcgaccgtg aagtcccccg tgcacaccgt
     3421 tccgcacgag tcgatgcaca gcacctgcac cgtcaccgtc tggtcggccg tgggcgtgta
     3481 gacccaggcg ccgctctgga tggagccggg gccagtgatg atctcgcacc ggtcgaggtt
     3541 gccgtcgacg tcggtcgcgc tcaccggaag ctggacctgc gccggcagac actgcgcgat
     3601 ggtcgtgtcg ttcggcaggt ggcacaccgg cgcgctgttg atctcgaccg ccacgaacgc
     3661 ggtgtcctcg tcggcctccc cgcacacgtc aaccgtccgc aggatgaacc cgtagatgcc
     3721 ggccgtgtcg ggcgtgaaac agatctgccc gccgctgtac gtccccgcgg gcgtgatcag
     3781 gtagcagctg tcgaggttgt tgtccgggtc cgtgcagccc gccggccagc agagcggcgt
     3841 gctcgcgcac aggaacaact gctggtccgc cccggcgttc gccgtcggcg cgtagttgat
     3901 ctgcaccgtc acgtcgagcg tatccaggtc gaatgcgccg caggcatcct cgacccgcac
     3961 gatgaaccgg taggtgcctg aggcgggggc cgtgaagcac agctcgttgg ccgccgtgtc
     4021 cagggtcgcc gtcccgctga ccaactcctc gcggacgacg ttgttgtccg ggtcggtcac
     4081 cgtgtacggc aggcactgct cgctgggatc gcactggaag agcgtgagct tgttgacctt
     4141 gccgccgccg aggtcgacca cgggcggcgt attgaatgtc agattatagg tcatcgtcgc
     4201 cgtatcggcc gcgccgcacg gatcggccac aatgaccgtg accgggtacg cgccgccctc
     4261 ggtcggcgtg aaacaccata ccccggccgg cgtcaccgtc cccggcccgc tcaggcgcgt
     4321 ccaggtcagc ggatcgccgt ctgcatccgc ggccgcgaac gcaaagcaga tctggcccga
     4381 ggcgcagagg aaactgtcga ccggatcggg caggtcggcc gccggcgccg tgttgatcga
     4441 cacgtcggcg atcaccgtgc aggtgtccgc cccgcaggtg gtcgccgcaa tcaccgtcag
     4501 ttcgtagtgg ccgctggtgt cggcgaagaa gcagacgttg ccgcccgcgt accacccgat
     4561 cgggctgacc gtcaccgtgt cgctccgcat caggaccgtc acgggcacac acaccgtgtc
     4621 gggcgtgcag atgtcgaacg tttgcggccc ggggcagctg atctgcgcgg ccgcgccgat
     4681 gtcgaccgtg aactcaagga tgcaggtgtc ggcgttgcag tcggtcgagg caatgacctc
     4741 gatcgcgtaa gtcccggtcg tgtccgccgg gaagcagagc tgcccgccgc tgtacgtgcc
     4801 gtgggacgtg ctcaccgtcg cgctgccggg cgtgatgccg agcaggtagc agacctcatc
     4861 cggtccgcag agcgagacgg cgatcggcgt gctcgggcag tcaatcgaga cgaaagtccc
     4921 gaacgccacc gtcaccacca tcgtatcccg gtcgctcgca ccgcacgaat ccgtcacccg
     4981 cacgacgaac tcgtagctcc cggcggcggc cggcgtgaaa cagatccggt tggccgccgt
     5041 gtcgatcgtg ccgaaacccg agagcatggt ctcggtgagc cgccccccct tcggatcgtc
     5101 ggccgcgtag tcaaagcaga cctcctgcgg cgcgcacagg aagaggctcg agtcgcccgg
     5161 cagctgcact gtcggcggct cgttgttcgt tactgtgatc gttaccgtgt ccgcccggta
     5221 caccccgcag gcatcggttg cctcgagaat aaaatcgtag tcgcccgagg tcgtcgggtt
     5281 gaaacagatc tcgtccccgt cgaacgtccc cgggccgatc agattcacgc tcgtcaggtt
     5341 gttgttggcg tcggtcaccg acgccggcca gcagatctcc gcacccgtct ggcagaggaa
     5401 gagatcctgg tcgctgccgg cgttcaccgc cggcggcacg ttgagcccga cggtcacgtt
     5461 gatcgtgtcg taatcagccc ggttgcagtg gtcggcgatg ccgatgatga aagtcgcggt
     5521 gcccggggcc gccggcgtgt agcaaacccg ggagttcgcc tggtcaagcg ttcccggccc
     5581 ggacacgagc gtgatggtcg tcgtccccag cccctccgga tcggaggcgg tgtagggcag
     5641 acagatttcg gcgagctggc actggaacgt gttgatgtcc gcgccgaagg cgatctgcgg
     5701 gggaccgttg acgtcaaaat cgaccgtgaa ctgcgattcg cagtaggccc cgcaggaatc
     5761 ggtgcagcgc atcaccactg tcaccgtctg gtcgctggcc ggcgtgtagc accactggcc
     5821 gttcaccagc gagcccggac cggaaataat ctggcacatg tgcaggttgc cgttggcgtc
     5881 ggtcgcgtaa gccggcagac agacctcctg cgccgcgcac tgggcgatgc tcgtgtcgct
     5941 cggcgccacg cagaccgggg cctcattgag cgccaccgtt atcgtcgccg aatcgtaatc
     6001 cgccgccccg caggcgtcgg tcgcccgcag cacgaacagg tacgatccgt cggcggacgg
     6061 cgtgaagcag atttgcgagc cgttgaacgt ccccgggccc tgcgtgagcg tcacgctcgc
     6121 caggttcccg tcggggtcgc tccccgcggc cggccagcag atcggcgcgg gcgcgcagag
     6181 gaagagcgtc tggtccgctc ccgcatcggc cgtcggggcg ctgttgagcg tgtagtacac
     6241 gagcgccgtg tcctcgtcaa ccgccccgca cgcatcggtt gcccgcacga tgaactggta
     6301 cgtccccgtg cccgtcggcg taaagcacac ctggttgccg accagggtgc cggggccgct
     6361 gaccagggtc accgcgctga ggttgttgtc cggatcgctg ctcgccaccg gcagacagat
     6421 ctgcgccggt gcgcatgcaa acagcgtctg gtcggctccc agactcaccg ccggcgagct
     6481 gttgagcccg accgtgacca ccatcgtgtc gcggtcgctc tgcccgcagg cgtccgtgac
     6541 ccgcaggata aacgtgtacg aaccggcccc ggcgggcgtg aagcagaacc ggctgttggg
     6601 ctggtcgagc accgcccccg gcgcgccggc gagaatctct atcagtgtaa tgttgccgtc
     6661 cgcatcggtc gccgcgtacg gcaggcacat cgccgtcgcc gcgcactgga agaccgtcgt
     6721 atcgttgccc agcgccatga ccggcgcgct gttgatcgcc acctcgtagg tgaggctgac
     6781 cgtgtccttc gccccgcacg gatcggccgc caccgccgtg acgctgtggc tgccggccgc
     6841 cgcggtgaaa caccacagac ccgaggaact cacggttccg ctccccgaaa gacggctcca
     6901 ggtgagcgga tcgccctcgg cgtcggccgc cgcgaactgg tagcacacct gcgccgccgc
     6961 gcacatgaag gtgtcgaccg gcgaggacgg atcggccgcc gtcggcggcg tgttgatcgt
     7021 cacgtccacc gtgaacgcac aggtgtcggc cccgcacagc gtgttggcga tcaccgtcag
     7081 cgcgtagtgg ccgctcgtgt cggcgacgaa acagacgttg ccggccgagt accagccggt
     7141 cggcgtgacc gtgaccgaga ggttgccccc gttgatgctg accggcacgc agaccgtgtc
     7201 ggccgcgcac agcgactccg tctgcggctc agggcaggtg atcgaagccg ccgcgccgat
     7261 gacgacgttg aaagtgagca ggcaggtgtc ggcgccgcag tcctccgagg ccaccacctg
     7321 aatgtggtag gtcccgctcg tatccgcctg gaaacagagc tggccggccg cccacgtccc
     7381 gaacgaggcc gtcaccgccg ccgtgggcgg cgtcaccgcg agcatctggc agacttcgtc
     7441 ggccgcgcag agcgacacgt tgatcggccc ggtcgggcag tcgatcgccg cgacgtcgcc
     7501 gaaactcacc agcaccaggg ccgtgtcgag gtcgctgtcg ccgcaattgt cagtcgcttg
     7561 cacgatgaac tggtagagac cgccgctcgt cggcgtgaaa cagatttgat tggcggccgt
     7621 gtcgatcgcg ccgtacccgg acaccattgt ctcggtcacc aggtcgcccg cgtcaggatc
     7681 gcccgccgcg tagctcaggc agatcggctg cggcgcgcac agcgccaggg ccgtgtccgc
     7741 acccagcgcg atcgtcggcg gctggttgat cgtgatgttg taggtgtggc tcaccgtgtc
     7801 ggcatccccg caggaatccg ccacctgggc cgtcaccgcg taggcgccgg cgccggt
//
LOCUS       DAOOKS010000007         7844 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_10890_length_7844_cov_3.984337, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000007 DAOOKS010000000
VERSION     DAOOKS010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7844
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10890_length_7844_cov_3.984337"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /locus_tag="PK112_00230"
     CDS             complement(<1..899)
                     /locus_tag="PK112_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HPC10508.1"
                     /translation="MPNNVQVVEVESRAQLRDFITYPNRLYRDDPCYVVPLLSERLEF
                     FDFEKNPFYRAARVRLFLAKRGEVTVGRIATCINYAHNEFHGEKTGFFGFFDCPDDYD
                     VAFVLLKVAMIRLKMEGMEKMRGPMNFSTNHEIGFLVEGFDEPPRIMMPYNPPYLPEL
                     AARFGLRKAMDLLAYKLTDETPIPERVETVIGKIRQRGNLVVRPIRLRDFDNEVDRVN
                     QVYNQAWQNNWGFVPMGDAEFRWIARNLRQVIDPDLALLCEHEGRPVAFILGIPDINQ
                     ALARLRGRLFPLGLLRLLWHTKIR"
     gene            complement(906..2045)
                     /locus_tag="PK112_00235"
     CDS             complement(906..2045)
                     /locus_tag="PK112_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HPC10509.1"
                     /translation="MGIYPYFHPLQSAPGNEVIVDGKKCIMVGSNNYLGLVDHPKVKE
                     AAAEAARKFGSGCTGSRFLNGTLDLHLELEERLARFMQRPAALVFSTGFQTNLGTIAT
                     LVGKNDAVVIDRSDHASIVDGTRLSFGKVYKFAHNNTEDLDRVLARVRREITRGGVII
                     VVDGVFSMEGDIINLADLVPVAEKYNARVLVDDAHAVGVLGKTGAGTAEHFGLIDRVG
                     ITMGTFSKSFASLGGFIAADWEVINYIKHRARALIFSASIPPSNAAAVLAALDIIQAE
                     PERRERLWKNALRMQKEFRDLGFNICHSQTPIVPLVVGDDLDAFAFWKALFDNGVFTN
                     PVISPAVPPGQAMIRTSYTATHTDDQLDRVVEVIAKVGREKGIIP"
     gene            complement(2506..3588)
                     /gene="serC"
                     /locus_tag="PK112_00240"
     CDS             complement(2506..3588)
                     /gene="serC"
                     /locus_tag="PK112_00240"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018701774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HPC10510.1"
                     /translation="MAHRVYNFNPGPAVLPLEVLETVQKELLDYKGTGMSILESSHRA
                     AEYTEVNDQCIALARELFGLPADYHVLFMTGGASTQFALVPMNFLNGKTGAYIDTGSW
                     SNKAIKEAKIVGKAHVCYSGKEGGYKHIPTQKELDIPADAAYLHMTTNNTIYGTQFSS
                     IPDSKGLPLIADMSSDIASRRLDFTKFDLIYAGAQKNIGPAGVTLVLLRDRLLKTANP
                     NLPTMFKYGTHAEEKSLYNTPPAFGVYVMKLVFEWIKRQGGLAAVEKVNLAKKERVYQ
                     IMDLYPDYYRGHCDKESRSWMNITLRLPTEDLEKKFLAEAKAHGFIGLKGHRSVGGIR
                     VSAYNALPLEGVDKLVAFMEKFKKQN"
     gene            complement(3616..4494)
                     /locus_tag="PK112_00245"
     CDS             complement(3616..4494)
                     /locus_tag="PK112_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018053188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HPC10511.1"
                     /translation="MLILISDAFDASLPGILSRFGEVTDDKSRLADAEIVLIRSKTKV
                     TREYIDSAPRLRYVIRGGVGLDNVDVAYARTKGIKVDNTADASTTAVAELAFALMIAL
                     PNHIAAADASMRQGKWLKNELKRTELYGKTLGILGIGRIGLSLAVRARAFQMRVLGWH
                     PDVYFTDFAEICPTMEEVLERSDYVSLHMPLVDSTRGIINKETLKRFKDGAYLINTGR
                     GKCIVEQDVVDALKSGKLRGYATDVWNSDPPENSPLLGAPNTILTPHIGASTKENMIR
                     IGIIVERLIEDYTAKK"
     gene            complement(4512..5756)
                     /locus_tag="PK112_00250"
     CDS             complement(4512..5756)
                     /locus_tag="PK112_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007039214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HPC10512.1"
                     /translation="MSIVRPFKGLRPRPEYAGQVAAPPYDVLSSDEARAMAAGNPISF
                     LHVNKPEIDFPPDQDPTAPAVYEAGRKNLQALVDRGVMCRDTSPCLYLYRLTWRGRSQ
                     TGLWALTSCDEYNRNLIKKHEHTRPAKVSDRADHMETLGAQVGPVFSCFRHNEEIRRL
                     FDHVKKVPPMIDITTEQGVRHELWVISHRDTLDSLAAAFGRLPYLYIADGHHRSAAAA
                     EVAGRRAAKNPHHSGDEHYNFFMNVIFPDDELRILPYNRVVRSLNGMTLEELFARAGD
                     TFTFAPSDAPVEPQKPYEFGLYAAGRWYRMSARPGKVAAAGPAEAIDSAVLTRHFLTP
                     YLGIKDIRTDDRIDFVGGIRGVNELERLVDSGAFAIAFSLFPATVDQLLAVADAGEVM
                     PPKSTWFEPKLRSGLVVNYLDE"
     gene            6240..6506
                     /locus_tag="PK112_00255"
     CDS             6240..6506
                     /locus_tag="PK112_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10513.1"
                     /translation="MAEDVEKPDRGAGHRTRAQKWQEGHNLRDTSMQCAQCGEEIKGK
                     PVKQEGEIYCSLECAYLAAGLEPEEDEDYFEEDPVEGTFGDDDE"
     gene            complement(6705..7181)
                     /gene="greA"
                     /locus_tag="PK112_00260"
     CDS             complement(6705..7181)
                     /gene="greA"
                     /locus_tag="PK112_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HPC10514.1"
                     /translation="MSEPIYMSKDGVRKLEVELTRLKFEERPKIVADIKRAREHGDLS
                     ENAEYHAAKEAQAHLERRIAELELKLSRVQTIDTDAIPSDRVYLYAKVLVEDMVDGEQ
                     IQYAVVPPAEADVDNDIISVESPIGKGLLGKAEGEVAEIAVPAGMLRYKILKISRD"
     gene            complement(7197..>7844)
                     /locus_tag="PK112_00265"
     CDS             complement(7197..>7844)
                     /locus_tag="PK112_00265"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10515.1"
                     /translation="FCCGCKSCECDDEFGLFLTTVTGMYARAILPELADPVSSYPALA
                     FHLLPPGLLGIFALGLLATVMSTVDSYAHIAATTFGNDIVGRAARLKAGGILFYSRLG
                     LVISSVLAILVAIFFRSVVDIWHAFGSVGTPALLVPVVTSFVGRRRLRPAWALLSIVA
                     CGGLSALWLAASYVRADGGYWFGIQPILPGLVLSLLLFALFSRPVAPGNLPTPPD"
BASE COUNT         1470 a         2346 c         2647 g         1381 t
ORIGIN      
        1 cggatcttgg tgtgccagag cagccggagc agcccgagcg ggaacaggcg gccgcggagg
       61 cgggcgagcg cctggttgat gtcggggatg ccgaggatga aggcgaccgg gcggccctcg
      121 tgctcgcaca gcagggcgag gtcgggatcg atcacctgcc ggagattgcg ggcgatccag
      181 cggaactcgg cgtcgcccat cggcacaaac ccccagttgt tctgccaggc ctggttgtag
      241 acctggttca cccggtcgac ctcgttgtcg aagtcgcgga ggcggatggg gcggacgacc
      301 agattgccgc gctgccggat cttcccgatc accgtctcca cgcgctcggg gatcggggtc
      361 tcatcggtga gtttgtacgc caggaggtcc atcgccttgc gcaggccgaa acgggccgcg
      421 agctccggca agtacggcgg gttgtagggc atcatgatgc ggggcggctc gtcaaaaccc
      481 tcgaccagga agccgatttc gtggttggtg gagaagttca tggggccgcg catcttctcc
      541 atcccctcca tcttgagccg gatcatggca accttgagca ggacaaacgc aacatcgtag
      601 tcgtcggggc agtcgaagaa gccgaagaac ccggtcttct cgccgtggaa ctcgttgtgg
      661 gcataattga tgcaggtggc gatccgcccc accgttacct ccccccgctt ggcgagaaag
      721 agccgcaccc gggcggcccg gtagaacggg ttcttctcaa aatcaaagaa ctccaggcgc
      781 tcgctcagca gcggaacgac gtagcacgga tcatcccggt agaggcggtt gggataggta
      841 atgaaatccc gcaactgcgc gcgcgactca acttcgacga cctggacgtt gttcggcata
      901 ggcgtctacg gaatgatccc cttctcgcgg ccgaccttgg cgatcacctc gaccacgcgg
      961 tcgagctggt cgtcggtgtg ggtggcggtg taggaggtgc ggatcatggc ctggcccggc
     1021 ggcacggcgg gagaaatcac cgggttggtg aaaacgccgt tgtcgaagag agctttccag
     1081 aaggcgaacg catcgaggtc gtcgccgacg acgagcggga caatcggggt ctgcgagtgg
     1141 cagatgttga agcccaggtc gcggaactcc ttctgcatgc gcagcgcatt cttccagagg
     1201 cgctcgcgcc gttccggttc cgcctggatg atgtcgagcg cggccagcac ggcggcggcg
     1261 ttggacgggg ggatggaggc ggaaaagatc agcgcccgcg cccggtgctt gatgtagttg
     1321 atgacttccc agtcggcggc gatgaatccg ccgagcgagg cgaacgattt cgagaacgtg
     1381 cccatggtga tgccgacgcg gtcgatcagg ccgaagtgct cggccgtgcc ggcgccggtc
     1441 ttgccgagaa cgcccaccgc gtgggcgtcg tcaaccagca cccgcgcgtt gtacttctcg
     1501 gccaccggga ccaggtcggc gagattgatg atgtcgccct ccatggagaa gacgccgtcg
     1561 acgacaatga taacgccgcc gcgggtgatc tcgcgccgga cgcgggcgag cacccggtcg
     1621 agatcctccg tgttgttgtg ggcgaacttg tacaccttgc cgaaggagag ccgggtgccg
     1681 tcgactatgc tcgcgtggtc gctgcggtcg atcacgacgg cgtcgttctt acccacgagc
     1741 gtcgcaatcg tccccaggtt ggtctggaaa ccggtggaga agaccagggc ggccgggcgc
     1801 tgcatgaacc gggccagccg ctcctccagc tccaggtgca ggtcgagcgt gccgttgaga
     1861 aagcgcgaac cggtgcaccc ggaaccgaat ttgcgggccg cctcggccgc cgcctccttc
     1921 accttgggat ggtcgaccag gccgaggtag ttgttggagc cgaccatgat gcacttctta
     1981 ccgtcgacaa tcacctcgtt gcccggcgcc gactgcaggg gatggaaata gggatagatc
     2041 cccatcgcct gcaccttctg cggttcggtg tacgcgtagc acttctcgaa caggtccggg
     2101 tatgacgtca ccacagcggc cttcttctcg gctggcaagt caactccttt gttgcggttg
     2161 gcacactccg tccgaaccgt ctatgaaagc cattctgggt atgtttgtca agttaatagc
     2221 cggttcgctt cgggagatcg ttacctcttg cgaaataaca tcttagagct gaactccccc
     2281 cgcctggccg ccctcacccg cccaccggaa tcgaaggccg ggcgcaccct cgcgagggac
     2341 ggtgcgagcg aaatccacga atgcgatcgc cccgcttgcc gccggcgatc cggaaccggg
     2401 actcccggcc cgatgcggcg ccggcgggcc gcccggtcgg gccggataaa gaaagcggcg
     2461 aggctggggc ctcgccgcgg atgttgaccg gatgcacggg ccgggtcagt tctgcttctt
     2521 gaacttctcc atgaacgcca cgagcttgtc gacgccttca agcggcaggg cgttgtaggc
     2581 ggagacccgg atgccgccga cactgcggtg gcccttgagc ccgatgaacc cgtgcgcctt
     2641 ggcctccgcg aggaacttct tctcgagatc ctcggtggga aggcggaggg tgatgttcat
     2701 ccaggagcgg ctttccttgt cgcagtggcc gcggtagtaa tcggggtaca gatccattat
     2761 ctggtacacc cgttccttct tggccaggtt gaccttttcg accgccgcca ggccgccctg
     2821 ccgtttgatc cactcgaaga cgagtttcat gacgtagacg ccgaatgccg gcggcgtgtt
     2881 gtagagcgac ttctcctcgg cgtgggtgcc gtacttgaac atggtcggca ggttcggatt
     2941 ggccgtcttc agcagccggt ccctcaggag gacgagggtg acgccggcgg ggccgatgtt
     3001 cttctgggcg cccgcgtaga tgaggtcgaa cttcgtgaag tcgagccgcc gcgaggcgat
     3061 gtcggaggac atgtcggcga tcaacggcag ccccttgctg tccggaatgg aggaaaactg
     3121 ggtgccgtag atcgtgttgt tggtggtcat gtggagatag gcggcgtcgg cgggaatatc
     3181 gagttccttc tgggtcggaa tgtgtttgta gccgccctcc ttgccggagt agcagacgtg
     3241 ggctttcccc acgatcttgg cttccttgat cgccttgttg gaccaggagc cggtgtcgat
     3301 gtaggcgccc gtcttgccgt tcaggaagtt catggggaca agtgcgaact gggtggaggc
     3361 gccgccggtc atgaacagaa cgtggtaatc cgcgggcagg ccgaagagct cgcgcgcgag
     3421 ggcgatgcac tggtcgttga cctcggtgta ctcggccgcg cggtgggaac tctcgagaat
     3481 cgacatcccc gtgcccttgt aatccagaag ctccttctgg acggtctcga ggacctccag
     3541 ggggaggacg gcgggcccgg gattgaaatt gtagacacga tgagccataa gattttctcc
     3601 ctatgccgtg agcggttact tcttggccgt gtagtcttcg atgagccgct cgacaatgat
     3661 gccgatgcgg atcatgttct ccttggtgga ggcgccgatg tgcggcgtaa gaatcgtgtt
     3721 cggagcgccc agcagcggcg aattctctgg cgggtcggaa ttccacacgt cggtggcata
     3781 cccgcggagc ttgccgctct tgagggcgtc cacgacatcc tgctcgacga tgcacttgcc
     3841 gcggccggtg ttgatcaggt aggcgccgtc cttgaatctc ttgagcgtct ccttgttgat
     3901 gatgccgcgg gtggaatcga cgagcggcat gtggagcgac acatagtcgg agcgctcgag
     3961 cacctcttcc atggtcgggc agatctcggc gaagtcggtg aagtagacat cggggtgcca
     4021 cccgagaact ctcatctgga aagcgcgggc gcggaccgcc agcgacaggc cgatccggcc
     4081 gatgccgagg atccccagcg tcttgccgta cagctcggtg cgcttgagct cgttcttcag
     4141 ccacttcccc tggcgcatgg acgcgtcggc ggcggcaatg tggttgggca gcgcaatcat
     4201 gagggcgaac gccagctcgg ccaccgcggt ggtggaggca tccgccgtgt tgtcgacctt
     4261 gatccccttg gtccgggcgt aggcgacatc gacgttgtcc aggccgacgc cgccccgaat
     4321 gacgtagcgc agacggggag ccgagtcgat gtactcccgc gtcactttcg tcttgctccg
     4381 aatcagcacg atctcggcgt cggccagacg ggacttgtca tcggtcacct cgccgaaccg
     4441 ggacaaaatg cccggcaggg aagcatcaaa cgcgtcggaa atgagaatca acatacatcc
     4501 atccctttca tctattcatc gaggtagttc accacgaggc cactacggag tttgggttca
     4561 aaccaggtag acttgggcgg catgacttcg ccggcgtcgg ccaccgccag cagctggtca
     4621 accgtcgccg ggaaaagcga gaacgcgatg gcgaaggcgc cggagtcgac caggcgctca
     4681 agctcgttca cgccgcgaat gccgccgaca aagtcgatcc ggtcgtcggt gcgaatatcc
     4741 ttgatgccaa gatagggcgt gagaaagtgc cgggtcagga cggcggagtc aatggcctca
     4801 gccggaccgg ccgccgccac cttccccggc cgggcgctca tccggtacca gcggccggcg
     4861 gcgtagaggc cgaactcgta cggtttctgc ggctcgaccg gagcgtccga cggcgcgaac
     4921 gtgaaggtgt cgccggcccg cgcaaagagc tcctcgaggg tcatgccgtt gaggctccgg
     4981 acgacgcgat tgtacgggag aatgcgaagc tcgtcatcgg gaaagatcac gttcataaag
     5041 aagttgtagt gttcgtcgcc gctgtggtgc gggttcttcg ccgcccggcg gcccgccacc
     5101 tcggccgccg cggccgaccg gtggtggcca tcggcgatat acaggtacgg cagccggccg
     5161 aaggccgccg ccaggctgtc gagtgtgtcg cggtggctga tcacccagag ctcgtggcgc
     5221 acgccctgct cggtcgtaat gtcgatcatc ggcggtacct tcttgacgtg gtcgaaaagc
     5281 cgccggatct cttcgttgtg ccggaagcag gagaagaccg gtcccacttg ggcgccgagt
     5341 gtctccatgt ggtcggcccg atcggagact ttggccggac gggtgtgctc gtgtttcttg
     5401 atcaggttgc gattgtattc atcgcacgac gtcagcgccc agagaccggt ctggctccgc
     5461 ccccgccagg tcaaacgata caggtagagg cagggggagg tgtcgcggca catgacgccg
     5521 cggtcgacca gcgcctggag attcttgcgc ccggcctcat aaacggcggg agcggtcggg
     5581 tcctggtccg gcgggaaatc aatttccggc ttgttcacgt ggagaaatga aatcgggttg
     5641 ccggcggcca tcgcgcgcgc ctcgtccgag gacaggacat catacggcgg cgcagcgacc
     5701 tggccggcgt attcgggtcg ggggcgaagc cccttgaagg gcctgacaat cgacattgca
     5761 atcctcgctt tggggcctaa tgtgaacgat ttcacaactt gcgtacaata taggacgcag
     5821 gaatgcgagg tcaaccgaaa tcgggcgcgc ctgcaggcgg cggccgtact ccgcgccgca
     5881 cgcggctacc cataggaaaa ccggcgcaat cgggaccgcc ggaacaggaa aacgcgggaa
     5941 cgcggcggcc gcctccggag cggcggaagg gtgcgcagcc ggtcgaattc cgtcatagtt
     6001 cgactgaaat tgacgcaccg gacgctccct gaacccggcc ggtggctcag ccggatgaat
     6061 tcgccgcttg accgcgcgtg ccggcccgga agtcgtgcgg cgggcgggct ttcggggcgc
     6121 gccgggagag gtcaaaaacc gcgcccgaca tggaaaaatc agttgcgcgg ggaaattcac
     6181 ccgttctata tggggactga aaacggccgc ccgacagagt catcggccgt aaatcagcga
     6241 tggcagaaga cgtcgaaaaa cctgaccgcg gcgcgggtca ccgcacccgc gcacaaaagt
     6301 ggcaggaagg tcataacctg agggatacca gcatgcagtg cgcacagtgc ggcgaagaga
     6361 tcaaagggaa accggtgaaa caagaggggg aaatatactg ctcccttgaa tgcgcttatc
     6421 ttgccgccgg actcgaaccg gaagaagacg aggactactt cgaagaagac ccggtggagg
     6481 ggacgttcgg ggacgacgac gagtgattga gcggcgccgc cgggggaggc gcgccgcaag
     6541 ggaatcgggg gagcttcgcg cagggactca agggatcaca cggacccgcg ggcaggttgc
     6601 ccgcctcggg ggagggccgc gatgggttgc tcgttcccgg cgccgggaaa aacccccgcc
     6661 atcgaactcc ctgcggaacg caggcggccc aacgcgggtg ccgctcagtc gcgcgagatc
     6721 ttcagaatct tgtatcggag catgccggcg ggaacagcga tttccgccac ctccccctcc
     6781 gctttgccca gcagaccctt gccgatcggg gattcgacgg aaatgatgtc gttgtcgacg
     6841 tcggcctccg ccggggggac gacggcatac tgaatctgct ccccgtcgac catgtcctcc
     6901 accagcactt tcgcatagag gtacacccgg tcggagggga tggcgtcggt gtcgatcgtc
     6961 tggacccgcg agagtttgag ctccagttcg gcgatgcgtc gctcgaggtg ggcctgggcc
     7021 tctttggcgg cgtggtattc ggcgttttcg gacaagtcgc cgtgctcgcg ggcgcgcttg
     7081 atgtcggcta cgattttcgg tcgttcctca aacttcaggc gggtcaactc cacctcgagt
     7141 ttacggacgc cgtctttgga catgtaaatc ggttcgctca tacgccttat aatacgctaa
     7201 tccgggggag ttggcaagtt tccaggggca acggggcggg aaaagagggc aaagagcagc
     7261 agcgaaagca caaggccggg gagaatcggc tggatcccga accagtagcc gccgtcggcg
     7321 cggacatagc tcgccgccag ccagagcgcc gacaacccgc cgcacgcgac aatcgataac
     7381 aaagcccacg cggggcgaag ccgccggcgc cccacaaatg aggtaaccac cggcaccagc
     7441 agggcgggcg ttcccaccga cccgaatgcg tgccagatgt cgacgaccga gcggaagaag
     7501 atggcgacga ggatggcgag gacggacgag atgacgagcc ccagccgcga gtagaacagg
     7561 atcccccccg ccttgagccg tgcggcccgg ccgacaatgt cgtttccgaa cgtggtggcg
     7621 gcgatgtgcg cgtacgaatc gaccgtggac atcaccgtcg ctaacagccc cagcgcaaaa
     7681 atccccagca gaccgggcgg gaggagatga aaggccagcg cgggatacga cgacaccgga
     7741 tcggcgagct cggggaggat ggcccgggcg tacatgccgg tgacggtggt gaggaacaag
     7801 ccgaactcgt catcgcactc gcaagacttg caaccgcaac aaaa
//
LOCUS       DAOOKS010000008         7650 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_11300_length_7650_cov_3.670968, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000008 DAOOKS010000000
VERSION     DAOOKS010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7650
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11300_length_7650_cov_3.670968"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="PK112_00270"
     CDS             <1..224
                     /locus_tag="PK112_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10516.1"
                     /translation="TRLLRDYVLLAGGRADTPAQVREMFRERLPAYAVPVRVALIPEE
                     ARAGLKPDRKYLERVAQALYGQAGEKTAG"
     gene            complement(324..725)
                     /locus_tag="PK112_00275"
     CDS             complement(324..725)
                     /locus_tag="PK112_00275"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HPC10517.1"
                     /translation="MYTYRTAVKLHDTDAAGVLFFANYFRLAHEAYEAWMSAVGFDFF
                     HLIHEADYLVLIVRAECDFRRPLRVGDGVTVRMTASGIGRSSFTLAYTVVDDDDRVVA
                     QGATVHACVGKPDLNSVPLPAGLKTELEKLQ"
     gene            844..2733
                     /gene="pepF"
                     /locus_tag="PK112_00280"
     CDS             844..2733
                     /gene="pepF"
                     /locus_tag="PK112_00280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HPC10518.1"
                     /translation="MWLTSRVLSPVLLAVGLFLAAASAVISADSAIPQRADIADQYKW
                     RVADIFPTDSAWEAAFAGLQGSIGLFDQYRGRLGEGPATLLACLATRDSIELIADNLY
                     VYAYLILDEDNRVSTYQEMGGRIRQLWSRISEGTSFIEPEIIAAGKDKIVGFAGQDPK
                     LAVYRHYLDDLFRKQAHVLSDKEEALLALAGPAVSASGEIFNMVDNADIDYGTVADEN
                     GAEVALTKERVYKMLESTDRELRRRVSDRYNAAYLDYENTLAATLAASVKSDYFQAQA
                     RGYATCLESSLDRYNIPVAVFHSLIAAVNENLAPLHKWASLRKRILGVDTLFTFDLSV
                     PLMPEGRKEYTYEQAQEILRRGLQPLGPQFLTDLEKGFTSGWIDVYETEGKGSGAYSW
                     GTYSSHPYVLLNYNGTLASVFTLAHEMGHAMHSYYTNRHEPYISHNHSLFAAEVASTG
                     NEAVLMKYLLDNTTDRGDKMELLNYYIEQIIGSFFTQVMFSEFELTIHERIESGQALS
                     AEFMRKTYRDIYQKYWGPDLTIGAVNDLGCLRIGHFYSQYYVYQYATSYAAAQAMSQK
                     VLEDPKYLDTYMQFLAVGNSDYPVEILKKAGVDLTSPEPVQRTMKLFADLVDQMEALL
                     AESGK"
     gene            2801..3145
                     /locus_tag="PK112_00285"
     CDS             2801..3145
                     /locus_tag="PK112_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HPC10519.1"
                     /translation="MSDRVQQFRQEREKLNEIVLERDNLDIKRFFALDGAVYRDGALD
                     AKTKELLGLAASLVLRCDDCIAYHVIRARENRVTDAEFDEVMAIALVVGGSITIPHLR
                     RAARLWKEMKEE"
     gene            3142..4044
                     /locus_tag="PK112_00290"
     CDS             3142..4044
                     /locus_tag="PK112_00290"
                     /inference="COORDINATES: protein motif:HMM:NF027464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HPC10520.1"
                     /translation="MTRCVAALLAILAAAVPLRDSRGADPAPPAPFDSLLAWYRQTYS
                     FISMDDTIYTFETEFRLPEGFHRPDSAALTGYQYWVSHLPLWNQWKPVGVWKGGKAYE
                     YGEVSRVVHMPWRGTMYTDIGFPLRVFAEYCRYAGRESAMHIVTRADGDTLAYAAWLQ
                     SKVSYTPLGGPRLVPATPREASEMEFYRFLTVAMEQQTYQTLAANCDSLPPEEVRPGD
                     LVIGHDDRLLVGKVYIVVNMLVNDEGERMYTVATGCPKPNACDLHIPLLTPDRHSPWI
                     TPGHLAALVDDLPQWGFFRLRGSQ"
     gene            4130..4855
                     /locus_tag="PK112_00295"
     CDS             4130..4855
                     /locus_tag="PK112_00295"
                     /inference="COORDINATES: protein motif:HMM:NF016382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease V"
                     /protein_id="HPC10521.1"
                     /translation="MQYRQIEKWPVTRAEAAALQTALAREIRIAPAASEPRLIAAVDT
                     AYGRDGKRIYAAAVVVRYPGLEPVDRAEGCAPAIFPYVPGLLFFREGPAVIAALHRLA
                     TEPDMLMIHGHGLAHPARCGMAGLLGVLFNRPSTGCSRKLLAGTHRPVADAKGSWEPI
                     RIEGAEVGCAYRSKDRVKPIFVSPGHGCDLAGARELVVRCLRDYRLPEPLRLAHVFAN
                     KYKRYQENGTAGRKAGSPPPERT"
     gene            4858..6060
                     /locus_tag="PK112_00300"
     CDS             4858..6060
                     /locus_tag="PK112_00300"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HPC10522.1"
                     /translation="MPHSPDRPDRILAAAKRIAARQAALRRHLHQYPELSAREFRTSE
                     YLAAALRRLGLRLLATTTDTGVLAELQGVAGGRTVVVRSDIDALPITERTGLPFASKN
                     PGCMHACGHDMHMAVVWGVAAVLSGLRGQIAGTVRFIFQPSEEMPPGGARPMIESGAL
                     TDASAVFGLHVDPHVRTGKIGLRDGPTMAAVTDFDLIVRGRSAHAARPHLGVDAITTA
                     AEVIEAVQKVVAREIDPVEPAVITFGQIEGGVARNVVADEVRVAGTARTLSPRAEKAV
                     PRLIRRVAENIGRARGARVELRLIADYPVLSNDPRANRVLARNMERLYGRGKIETTEV
                     MLGGEDFACYLKQAPGAMVRLGVGNRKIKADQPWHSPAFIADEAALPVGTALLAAAVL
                     DVLSGGLA"
     gene            6057..7526
                     /locus_tag="PK112_00305"
     CDS             6057..7526
                     /locus_tag="PK112_00305"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HPC10523.1"
                     /translation="MKARTTIAGILAGLLLAVAAHAGYPEGQAVTFSDFSRTRSIAVT
                     VVRVYFATAQGIICYNQGEDRWEEPLPAIPGVDPEDISRIWADQFGRTLYAATPDGRY
                     VYDQTLAMWFLTSETPEGDGAAREVPPPAIMHAPPGFNYFAGGQLIDPYGRTWQINEM
                     VDDGSGMYWMAIWGYGPAKARTTNLIIDLLPFGLVQRRVNALDQEGGRLWVSGAVYDD
                     LRTGITGFEPAMMTFRHLESGVRPEFPADDINCLRSDSAHLYIGTARGLLVMDLESEM
                     IIRTVGERQGLAGENVLSIAVTGDSVFIGTDEGLNLVIGASDSAALILPTTFEGQYIW
                     DIEEVGDYVWVAAESGAYRLTKGAERLQRYRDPENVLFSHVYDIERWADKLWFVSDGG
                     LAELDLQTAESRSFHDMTQQLLPRALAVNETIAAVASDKGLTILFHTADKPFKRDFTV
                     DDGLASNNVYALAVDGDYLWVGTDRGLTRFLWNNPDRVD"
BASE COUNT         1334 a         2454 c         2629 g         1233 t
ORIGIN      
        1 cgacgcggct cctgcgggat tatgtccttc tcgccggcgg gcgggcggat actcccgcgc
       61 aggtgcggga gatgttcagg gagaggctcc cggcctacgc ggtgccggtg cgggtggcgt
      121 tgatccccga ggaggcgcgg gcgggattga aaccggaccg aaagtatctc gagcgggtgg
      181 cgcaagcact ctacgggcag gcaggagaga agaccgcggg gtagacggcg agcgttccct
      241 tttacgggag agagggccgc gaaaagaacg ctcctccgga agcttcagga tctccccgaa
      301 gggttgccgg cgccccgccc cccttactgc agcttctcca gttccgtttt cagtccggcc
      361 gggagaggga cggagttgag atccggcttg cccacgcagg catgcacggt ggcgccctgg
      421 gcgacgacgc ggtcgtcgtc gtccacaaca gtgtaggcga gcgtgaagga ggagcggccg
      481 atgccggaag cggtcatccg cacggtcaca ccgtcgccca cgcggagcgg gcgcctgaaa
      541 tcgcactcgg cgcggacgat gagcacgaga tagtcggcct cgtggatcag gtggaagaag
      601 tcgaacccga cggcggacat ccaggcctca taggcctcgt gggcgagccg gaaataattg
      661 gcgaaaaaga gcactccggc ggcgtcggta tcgtggagct tgaccgcagt ccgataggtg
      721 tacatggcgg tgaccttcaa gaaaagttcg tgcgcgacgc gcgggcggcg cgtatatttg
      781 cagcctatgg taacgccgaa agcaagcgtg gaacaagtga aatactcatc ccggagacct
      841 cctatgtggc tcacatcgcg tgtcctttcc cccgtcctcc tcgcggtcgg gctgtttctc
      901 gcagccgcgt ccgccgtcat ttccgccgac agcgccattc cccagcgggc ggatatcgcc
      961 gaccaataca agtggcgggt cgccgacatc ttcccgaccg acagcgcctg ggaggccgcc
     1021 ttcgccggtc tccagggaag catcgggctg ttcgaccaat accgcggccg tctgggcgag
     1081 ggaccggcga ccctcctggc ctgtctggcc acccgcgaca gcatcgagct gatcgccgac
     1141 aacctgtatg tctacgccta cctgatactc gatgaggaca accgggtgag cacctaccag
     1201 gagatgggag ggcgcatccg gcagttgtgg tcccggattt ccgaggggac ctccttcatc
     1261 gaaccggaga tcatcgccgc cggcaaggac aagatcgtcg gattcgccgg gcaggacccg
     1321 aagctggcgg tgtaccgcca ctacctggac gatctcttcc gcaagcaggc gcacgtgctg
     1381 tcggacaagg aggaggctct cctggcgctg gccgggcctg cggtgagcgc atccggagaa
     1441 atcttcaaca tggtcgacaa cgccgatatc gactacggca cggtggccga tgagaacggc
     1501 gcggaggtgg cgctcacgaa agagcgcgta tacaagatgc tcgagtccac cgaccgcgaa
     1561 ctccgccgac gggtgagcga ccggtacaac gccgcctacc tcgactatga gaacacgctg
     1621 gcggcgacgt tggcggcctc ggtcaagagc gactacttcc aggcccaggc gcgcggctac
     1681 gcgacctgcc tggagtcctc gctcgaccgc tacaacatcc cggtcgcggt cttccacagc
     1741 ctcatcgcgg cggtcaacga gaacctcgcc ccgctgcaca agtgggcctc gctgcgcaaa
     1801 cgcatcctcg gggtcgacac gctcttcacg tttgacctct ccgtgccgct catgccggag
     1861 gggcggaagg agtataccta cgagcaggcc caggagatcc tccgccgcgg cttgcagccg
     1921 ctggggccgc agttccttac tgacctcgag aaagggttca cgtcgggctg gatcgacgtc
     1981 tacgagacgg aggggaaggg gtcgggggcg tactcgtggg gaacttacag ctcgcacccc
     2041 tacgtgctgc tgaattacaa cgggacgctg gcctcggtgt tcacgctcgc ccacgaaatg
     2101 ggccacgcca tgcacagcta ctacaccaac cggcacgaac cgtacatcag ccacaaccat
     2161 tcgctcttcg cggccgaggt ggcctccacg gggaacgaag cggttctcat gaagtacctc
     2221 ctggacaaca cgaccgaccg gggcgacaag atggagctgc tcaactacta catcgagcag
     2281 atcatcgggt cgttcttcac ccaggtcatg ttcagcgaat tcgagctgac gatccacgag
     2341 cggatcgaga gcgggcaggc gctgtcggcc gaattcatgc gcaagaccta ccgcgacatc
     2401 taccagaagt actgggggcc ggacctgacg atcggggcgg tcaacgacct ggggtgcctg
     2461 cgcatcgggc atttctacag ccagtactac gtctaccagt acgccacctc ctacgcggcg
     2521 gcgcaggcga tgtcccagaa ggtgctcgag gatccgaaat atctcgatac ctacatgcag
     2581 ttcctggcgg tcggcaattc cgactacccg gtggagatcc tgaagaaggc gggggtcgac
     2641 ctgacctcgc ccgaaccggt ccagcggacg atgaagctgt tcgccgacct ggtggaccag
     2701 atggaggcgc tgctggccga gagcggcaaa taaggggcgg cggtccccgg ggcggcggca
     2761 acggccgccc cggcggcgcg gaaaaatatc ggagaaatcg atgtcggatc gggtacaaca
     2821 gttcaggcag gagcgggaga agctgaacga gattgtcctc gagcgggaca acctcgatat
     2881 caagcgcttc ttcgctctgg acggcgccgt gtaccgggac ggtgcgctgg acgccaaaac
     2941 caaggagctg ctcggactgg cggcctcgct ggtgctccgc tgcgacgact gcatcgccta
     3001 ccacgtcatc cgcgcccggg agaaccgcgt gaccgacgcg gagttcgacg aggtgatggc
     3061 gatcgcgctg gtggtcggcg gctccatcac cattccccac ctgcgccggg cggcccggct
     3121 gtggaaggag atgaaagagg aatgacgcgc tgcgtcgctg cgctgcttgc gattctggcg
     3181 gcggccgtcc cgctccggga cagccgcggg gcggatccgg ccccgccggc cccctttgat
     3241 tccctgctgg cctggtaccg ccagacctac tccttcatca gcatggacga cacgatctac
     3301 acgttcgaga cggagttccg tctccccgag gggttccacc ggccggattc ggccgccctg
     3361 accggatacc agtactgggt ctcccacctg ccgctgtgga accagtggaa gccggtcggg
     3421 gtgtggaagg gcgggaaagc gtacgagtac ggcgaggtct cgcgcgtcgt gcacatgccc
     3481 tggcggggga cgatgtacac cgatatcggg tttccgctgc gcgtgttcgc ggagtactgc
     3541 cggtacgccg gccgcgagtc ggccatgcac atcgtgacgc gggcggacgg cgacacgctc
     3601 gcctacgccg cctggctcca gagcaaagtg agctacacgc cgctcggcgg gccgcggctg
     3661 gtgccggcca cgccgcgcga ggccagcgag atggaattct accggttcct gacggtggcg
     3721 atggagcagc agacgtacca gacgctggcg gccaactgcg attccctccc ccctgaggag
     3781 gtgcgacccg gagacctcgt catcgggcac gacgaccgcc tgttggtcgg aaaggtctac
     3841 attgtggtca acatgctggt caacgacgag ggggagcgga tgtatacggt ggcgaccggc
     3901 tgccccaaac cgaacgcctg cgacctgcac attccgctgc tgacgccgga ccgccactcc
     3961 ccctggatca cgccggggca cctcgcggca cttgtcgacg atctcccgca gtgggggttc
     4021 ttccgcctgc ggggttcgca gtgacggtcc cgcgccttga cgggacggga ggttcttcgt
     4081 atcgtttcgc agaattcaga accgcagccc gcggtgttca caggcaggcg tgcagtatcg
     4141 gcagattgag aagtggccgg tgacgcgggc cgaggcggcg gcgctccaga cggcccttgc
     4201 ccgcgagatc cggattgccc ccgcggcctc cgagccccgc ctgatcgcgg cggtcgacac
     4261 ggcctatggc cgcgacggca agcggattta cgccgccgcc gtcgtcgtcc ggtatcccgg
     4321 cctggagccg gtggatcggg ccgaggggtg tgcccccgcg atttttccct atgtgccggg
     4381 gctgctgttt ttccgcgagg gaccggcggt catcgcagcg ctccaccgcc tggcgaccga
     4441 gccggacatg ctcatgatcc atggccacgg cttggcccac ccggcccgct gcggcatggc
     4501 cggcctgctc ggcgtcctgt tcaaccggcc ctcgaccggg tgcagccgca agctcctcgc
     4561 gggaactcat cggccggtag ccgatgcgaa agggagttgg gagccgatcc gcattgaggg
     4621 agccgaagtc gggtgcgcct accgttcgaa agaccgggtg aaaccgatct ttgtctcccc
     4681 cggacacggt tgcgatctgg ccggcgcccg ggaactggtg gtccgctgtc tgcgggacta
     4741 ccggttgccg gaacctttgc ggctggcgca tgtgtttgcc aataagtaca agcgctatca
     4801 ggaaaacgga acagccggcc gtaaggccgg atcaccccca ccggagcgaa cctgaacgtg
     4861 ccccactccc ctgaccgacc agaccggatt ctcgccgcgg cgaagcggat cgctgcccgc
     4921 caggcggcgc tgcgccgcca cctccatcag tatcccgaac tttccgcgag ggagtttcgg
     4981 acgagcgagt acttggccgc cgcgctgcgg cggctgggcc tgcggttgct tgcgaccacg
     5041 accgacaccg gtgtgctggc ggagttgcag ggggttgcgg gggggcggac ggtggtcgtg
     5101 cgcagcgaca tcgacgcgct gccgattacg gagcgcaccg gcctgccgtt cgcatcgaaa
     5161 aacccgggct gcatgcacgc ctgcggccac gatatgcaca tggcggtagt ctggggagtg
     5221 gcagccgtgc tgagcggtct gcgcggccaa atcgccggaa ccgtccggtt catcttccaa
     5281 ccctccgaag aaatgccccc cggcggggcg cggccgatga tcgagtcggg cgccctgaca
     5341 gacgcctccg cggtgttcgg tctccatgtc gacccgcacg tgcggacggg gaaaatcggc
     5401 ctgcgggacg gtccgacgat ggcggcggtg accgatttcg atctcattgt gcgcggccgg
     5461 agcgcccatg ccgcccggcc ccacctggga gtggatgcga tcacaaccgc cgccgaggtg
     5521 atcgaggcgg tgcagaaagt ggtcgcgcgc gagatcgacc cggtcgagcc ggccgtgatc
     5581 acgttcgggc agatcgaggg cggggtggcg cgcaacgtgg tcgccgacga ggtgcgggtg
     5641 gccgggacgg cgcggacgct gtcgccgcgg gccgagaagg cggtgccgcg cctgatccgc
     5701 cgggtggccg aaaacatcgg ccgggcccgc ggcgcccggg tcgagctgcg cctgatcgcc
     5761 gactatccgg tgctcagcaa cgacccgcgc gccaaccgtg tgctggcccg caacatggaa
     5821 cggctctacg gacgcggtaa aatcgaaacg accgaggtga tgctcggcgg cgaggatttc
     5881 gcctgctacc tcaagcaggc gccgggggcg atggtgcggt tgggtgtggg gaacaggaaa
     5941 atcaaggccg accaaccctg gcactcgccg gcgttcatag ccgacgaagc ggccctgccg
     6001 gtcggcaccg cgctgctggc ggcggcggtc ctggacgtcc tctcaggcgg gctcgcatga
     6061 aggcgcgcac gacgatcgcg gggatcctcg cgggactcct gctggccgtt gcggcccacg
     6121 ccggctatcc cgaggggcag gccgtgacct tctcggattt ctcccgaacc aggagcatcg
     6181 ccgtgaccgt cgtccgcgta tatttcgcca ccgcccaggg gatcatctgc tacaaccagg
     6241 gggaggaccg ctgggaggag cccctgccgg cgatcccggg agtcgatccg gaagatatca
     6301 gccggatctg ggccgaccag ttcgggcgca cgctctatgc ggcgacgccg gacgggaggt
     6361 acgtttacga ccagacgctg gcgatgtggt tcctcaccag cgagacgccc gagggggacg
     6421 gggcggcgcg cgaggtgccc ccgccggcca tcatgcacgc tccgcccggt ttcaactatt
     6481 tcgccggcgg acagctcatc gacccctacg ggcggacctg gcagatcaac gagatggtcg
     6541 acgacgggag cgggatgtac tggatggcca tctggggata cggcccggcg aaagcgcgga
     6601 ccaccaacct catcatcgac ctgctcccgt tcggactggt ccagcggcgc gtcaacgccc
     6661 tcgatcagga gggcggccgg ctgtgggtgt ccggggcggt gtacgacgac ctgcgcaccg
     6721 ggattacagg attcgagccg gcgatgatga ctttccgcca cctcgagtcc ggggtgcggc
     6781 cggagtttcc cgccgacgac atcaattgtc tccgctccga ttccgcccac ttgtacatcg
     6841 ggaccgcgcg cgggctgctc gtgatggacc tggagagcga aatgatcata cggacggtcg
     6901 gggagcggca gggactggcc ggcgagaatg tcctgagcat cgcggtgacc ggcgattcgg
     6961 tgtttatcgg gaccgacgag gggctgaacc tggtgatcgg ggcctccgat tcggccgccc
     7021 tgatcctccc cacgacgttt gagggccagt acatctggga catcgaggaa gtcggcgact
     7081 acgtttgggt cgccgcggag agcggcgcct accggctgac gaagggcgcg gagcgactcc
     7141 agcggtatcg cgatccggag aacgtgctgt tcagccacgt ctacgacatc gagcggtggg
     7201 cggacaagct ctggtttgtg tccgacggcg ggctggcgga gctggatctg cagacggcgg
     7261 agtcgcggtc gttccacgac atgacccagc agctgctccc gcgggcgctg gcggtcaacg
     7321 agacgatcgc ggcggtggcc tcggacaagg ggctgacgat cctctttcac accgcggaca
     7381 aaccgttcaa gcgggatttc acggtcgacg acgggctggc ctcgaacaac gtctacgcac
     7441 tggcggtgga tggggactac ctgtgggtgg ggaccgaccg ggggctgacc cggtttctct
     7501 ggaacaatcc cgaccgcgtg gactagttcc ggccggattt ccgcaattgt tggttgtttg
     7561 cggccgggac gtgtgttacc atatcggcgg ccggtcgccc gccgcccggc cgtgggaacc
     7621 gtgacatatg aaatccctgc tcgagaactt
//
LOCUS       DAOOKS010000009         7644 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_11313_length_7644_cov_4.393069, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000009 DAOOKS010000000
VERSION     DAOOKS010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7644
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11313_length_7644_cov_4.393069"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="PK112_00310"
     CDS             complement(<1..296)
                     /locus_tag="PK112_00310"
                     /inference="COORDINATES: protein motif:HMM:NF016829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HPC10524.1"
                     /translation="MELLSLPLLPDAGHFPRTALDRSIVYSIGLILTSRLGMMPFLPD
                     YGCDLWEREFTDLPTANKANIRASLRNSIDTHERRLYNVSVSFSEAQTSSGHALG"
     gene            complement(304..2199)
                     /locus_tag="PK112_00315"
     CDS             complement(304..2199)
                     /locus_tag="PK112_00315"
                     /inference="COORDINATES: protein motif:HMM:NF017739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HPC10525.1"
                     /translation="MTPEFDAEFRARRADVGADALARRAENVPLDYGTPVAALLRVAG
                     KEIKADIHSVRLNQHVFGHHVMQVLVRRVGLAEGGSGEFEQISESAGFLGKPVTLELR
                     PEGGVVAGDRRLEFIGLITEVRFDHTIDGINSVLITAHSPTISMDLVPRTRVSPPGGA
                     CGTIIGGILGEHPITQGRVQSSPVASAPAGLNLEVDSGVQYFETDYRYLRRLASEHQM
                     FAYYDGRAFHVEKAAANNEQVLKWREELGSFAVGIGTGPLAFRGQVWDPRRKEMVTGS
                     ADRSSIRAALSPLSRTALDGSERVFTTAGVSLRVKHPDQSKVDGALAREVEASAGQMM
                     LCRGTSIVPAVTVGSCVRVRGMDELDGQYWVTAVRHEVSDSGKYYNEFECTPLELALP
                     PLHTERHRRGYFQTGVVTDNNDPDGLGRVRVRLSWQAEDQASEFLRLLTFDGGQGRGW
                     FGLPEVGDEVLVAYERGNPDAPVVLGCLYNGQDTPPKATADCLDGGQVATKIFRTRRG
                     NELVFDDRDGTETITINQRDNTNKIVLAMDGPKIVIETTGDLLMKAAAIAVESTSGDI
                     SIKSAAALKGEAAADIDLSAQANFKSEGAMNYEAKGGVEFKASATQATVEGSAMTTVK
                     GGIVKIN"
     gene            complement(2260..2670)
                     /gene="tssD"
                     /locus_tag="PK112_00320"
     CDS             complement(2260..2670)
                     /gene="tssD"
                     /locus_tag="PK112_00320"
                     /inference="COORDINATES: protein motif:HMM:NF037603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
                     /protein_id="HPC10526.1"
                     /translation="MAHRPKLEIDGVEYPHVYSVSYHVYTAKDETGRPTDRAHAGLIK
                     IKVESAEDGNVDIARWAMDTSKPNWKSGTVVFFNKDGAVMKELSWTDGFITDFRETIP
                     DVKNNPDEQIYQEFEISSNAIMIGDAELQNYWDK"
     gene            complement(2755..4176)
                     /locus_tag="PK112_00325"
     CDS             complement(2755..4176)
                     /locus_tag="PK112_00325"
                     /inference="COORDINATES: protein motif:HMM:NF036663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10527.1"
                     /translation="MGEEEKRAASSGPQADAKPAKAKELLSDEEFAGILGDAFGDFKK
                     FAGLLPSFKTADGDLVRGVEWLDPKKEFQRKEFLTKPDHARQRRLLAERLKLWIEMLA
                     EGGSIDDLRKKLNEKAAQLESNLEKNMKKVHKAARDLETGYRAIDKFFANAQAEPDAK
                     VNAWFANVSAEELMDPDDKEKFEQLSKAIAAEFREWGIKRCYSMCVVPGYLGSVQNID
                     TLARQIGLPNRVHVVTDLPDLESVEDIMEMLEDPSMDGLMSSDECKQYVSLFANYLLA
                     RSANQYEDGDMWVPPSAAVAGKMYLGDETVGMQQPAAGYKYGKINEAKYLRFRANQPD
                     ASKVNEKGVNPLVSFEGQAVAMGSSTLSNKETFNVYSIRRTYDYVYKTLRNYLNKQTF
                     NVIDQKFIDTLRKDIDKFMRAISGGDNILQDYTVQVYADEEMRKRQEVDIKVALNPKY
                     PARTFNIEFTAWNEDNQTAVKDK"
     gene            complement(4194..4637)
                     /locus_tag="PK112_00330"
     CDS             complement(4194..4637)
                     /locus_tag="PK112_00330"
                     /inference="COORDINATES: protein motif:HMM:NF017409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HPC10528.1"
                     /translation="MANNFWLGARERKVKDDSIPVELLPSNKTLYVAKLNNDEAAGVE
                     PVECNNLKQVFEKFQPSFSAEVETLDGQSAPAEFKIRAMKDFQSKELIEQNEYLQKVY
                     FSKEILSDLDKQLKKNTALKKTLEDPQTREALLKAAKYYVELLSE"
     gene            complement(4665..5768)
                     /locus_tag="PK112_00335"
     CDS             complement(4665..5768)
                     /locus_tag="PK112_00335"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPC10529.1"
                     /translation="MARPELTLKDIREAFARQQQRCRDLAEVVRNLQPVIHESDIGAV
                     VARRTGIPLSKMMTAEKDRLINMEAHLASKIVGQEKAITSVANAIRKARAGLKLANRP
                     VGSFLFLGPTGTGKTYLPKLLAEFLFDDKNAMVRLDMSEFMEKHSVAKMIGAPPGYVG
                     YEEGGVLTEAVRRKPFSIVLFDEVEKAHPDVFNILLQLLDDGRLTDGQNRTVDFSNCI
                     VVLTSNYAADLILDADREGREVDMDQVREFLFTKFRPEFLNRLNDIIIYHTFTPAQVE
                     TIAGLEFAQLARMLAEQNIAVSLSPEARAKLARDGYTFELGARPIQRVIERDITNRLS
                     VEIITGTLAPGDAVVIAVQDDQYVFTRTGESRG"
     gene            complement(5768..7219)
                     /locus_tag="PK112_00340"
     CDS             complement(5768..7219)
                     /locus_tag="PK112_00340"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPC10530.1"
                     /translation="MDIAKYSSDSRAVIKVAREVAAEFRHPEISIEHLLVAVVRHEGS
                     EVESILNQLKKNPATVAAMVESYLKQQPSRAAARDSLSASPAVQAVLAQAFEEKGKLY
                     DALVEPEHILLAIFDRASALPPYLREQLEFTKEDLYQAMAESRSVEAIAVSPPRGEGS
                     AAPAEEPRTVAAVSRYCADLTGRAAAGEYDPMIGRAEELRQVIQILLRRRKNSPVLVG
                     GAGVGKSAIVEGLAQAVVSGAVPKALQGVKVLEVDMGGLVAGAKYKGEFEERFKALIG
                     EVIKSAGRLILFIDEVHTIMGAGSGGAGMDAANLLKPALARGQLRLVGATTEEEYTKH
                     LEKDKALLRRFEKVKVGEPPFDEAVAIVNGVAAKYEEHHRVTFSPEARVAAVKYAKRY
                     LSEKNLPDIALDIIDEAASEFGVKKEMAERLLPGLEAAMKDIEPLLAAADGAGLSDGD
                     QQKLRALYETFAGQLDALGGYWGHRLEVPEVSG"
     gene            complement(7398..>7644)
                     /locus_tag="PK112_00345"
     CDS             complement(7398..>7644)
                     /locus_tag="PK112_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10531.1"
                     /translation="LLQPVYSATVGDFTYEQCPYGADSCWFLENNPERDVDWVGYEFR
                     QGIGLVSTSVLYMSIDLVDYRIGSAGRDSLKAGNSSP"
BASE COUNT         1150 a         2301 c         2594 g         1599 t
ORIGIN      
        1 ccgagggcgt ggccggagct cgtttgcgcc tccgagaacg acaccgagac gttgtagagg
       61 cgccgttcgt gggtgtcgat ggagttgcgg aggctggcgc ggatgttcgc cttgttcgcc
      121 gtcggcaggt cggtgaactc gcgctcccac aggtcgcacc cgtagtcggg cagaaaaggc
      181 atcatgccga ggcgggaggt caggatcagc ccgatggaat acacgatgct gcggtcgaga
      241 gcggttctcg ggaagtggcc ggcgtcgggc aggaggggca gtgagagcaa ttccatgcga
      301 tacctagttg atcttgacga tcccgccttt gacggtggtc attgcgctcc cctcgacggt
      361 cgcctgggtc gccgaggctt tgaattccac gccccccttg gcctcgtagt tcatcgcgcc
      421 ttcgctcttg aaattggcct gggcgctgag gtcgatgtcg gcggccgctt cgcccttgag
      481 cgccgccgcc gatttgatcg agatgtcacc ggaggtcgac tcgaccgcga tggccgccgc
      541 cttcatgagc agatccccgg tcgtctcgat cacgatcttg gggccatcca tggccaggac
      601 gattttgttg gtgttgtcgc gctggttgat ggtgatcgtc tccgtgccgt cgcggtcgtc
      661 gaacaccagc tcgttgccgc ggcgggtgcg gaaaattttg gtcgccacct ggccgccatc
      721 gagacaatcg gcggtcgctt tcggcggcgt atcctgcccg ttgtacagac acccgaggac
      781 gaccggggcg tcgggattgc cccgctcgta ggccacgagg acctcgtcgc cgacctccgg
      841 cagtccgaac cagccgcggc cctggccgcc gtcaaaggtg agcaggcgga ggaactcgga
      901 ggcctggtcc tcggcctgcc aggagaggcg gacgcggacg cggccgagcc cgtcggggtc
      961 gttgttgtcg gtgacgacgc cggtctggaa gtacccgcgg cggtggcgtt cggtgtgcag
     1021 gggcgggagc gccagttcga ggggcgtgca ctcgaattcg ttgtagtact tgccgctgtc
     1081 ggagacttcg tgccgcaccg cggtgaccca gtactggccg tcgagttcgt ccatgccccg
     1141 cacgcgcacg cagcttccca cggtgacggc ggggacgatt gaggtcccgc ggcagagcat
     1201 catctgcccg gcggaggcct cgacctcgcg cgccagcgcc ccgtccacct tcgactggtc
     1261 ggggtgcttc acgcggagcg acacgccggc ggtggtgaag acgcgctcag aaccgtcgag
     1321 cgccgtgcgc gacagcggcg acagggcggc gcggatcgag gaccggtcgg cgctcccggt
     1381 caccatttct ttccggcggg ggtcccacac ctgtccccgg aacgccaggg ggccggtgcc
     1441 gatgccgacg gcgaaggagc cgagttcctc gcgccacttg agcacctgct cgttgttggc
     1501 ggcggctttc tcgacgtgga acgcccggcc gtcgtagtag gcgaacatct ggtgttccga
     1561 ggccaggcgg cggaggtagc ggtagtcggt ttcaaagtac tggacgccgg agtcgacctc
     1621 gaggttgagt ccggcggggg cgctggccac cggggagctc tggacgcggc cctgggtgat
     1681 ggggtgttcg ccgaggatgc cgccgatgat ggtcccgcag gcgccgccgg gcggcgacac
     1741 gcgggtgcgc ggcaccaggt ccatcgagat cgtggggctg tgggcggtga tgagcacgct
     1801 gttgatgccg tcgatcgtgt ggtcgaaacg gacctcggtg atcaggccga tgaattcgag
     1861 ccggcggtcg ccggcgacga cgccgccttc ggggcgcagt tcgagggtga ccggtttgcc
     1921 gaggaagcct gcggattcgg agatctgctc gaattctccg gatcccccct cggccagtcc
     1981 gacgcgccgg acgagtacct gcatgacgtg gtggccgaag acgtgctgat tgaggcggac
     2041 ggagtggatg tcggccttaa tctctttgcc ggccacccgg aggagggcgg cgacaggggt
     2101 gccgtagtcg aggggcacgt tttcggcgcg gcgggcgagg gcgtcggccc cgacgtcggc
     2161 gcggcgggcc cggaattcgg cgtcaaattc gggtgtcatg agtgacctgt cggccggccc
     2221 ggccgggcgg cacacggccg cccggccgcg gcgcgccgct tacttatccc agtaattctg
     2281 cagctccgcg tcgccgatca tgatcgcgtt cgacgagatc tcgaattcct ggtagatctg
     2341 ctcgtccgga ttgttcttga cgtccgggat ggtttcccgg aaatccgtga tgaaaccgtc
     2401 ggtccagctc agttccttca tcacggcccc gtccttgttg aagaagacga ccgttcccga
     2461 tttccagttg ggcttggagg tgtccatcgc ccagcgggcg atgtccacgt tgccgtcttc
     2521 cgccgactcg actttgatct tgatcaggcc ggcgtgggcg cggtcggtgg gccgcccggt
     2581 ttcatccttc gccgtgtaca catggtacga cacggagtac acgtgggggt actccacccc
     2641 gtcgatttcg agtttcgggc gatgtgccat ggcaatcctt ccttttccag tgtttgcgtt
     2701 ggaaagtcac cgttccgtat tcgcgacagt ttttgctcct gacgaccggg cggcctactt
     2761 gtccttcacc gccgtctgat tatcctcgtt ccaggcggtg aattcgatgt tgaaggttcg
     2821 cgccgggtac ttcgggttca gcgcgacctt gatgtccacc tcctggcgtt tacgcatttc
     2881 ctcgtcggcg tagacctgca cggtgtagtc ctggaggatg ttgtcgccgc cggagatggc
     2941 gcgcatgaac ttgtcgatgt ccttgcgcag ggtgtcgatg aatttctggt cgatgacgtt
     3001 gaaggtttgt ttgttcaggt agttgcggag cgtcttgtac acgtagtcgt aggtgcgccg
     3061 gatggagtag acgttgaagg tctccttgtt ggagagggtg gacgatccca tggcgaccgc
     3121 ctggccctcg aaactgacca gggggttgac gcccttctcg ttgaccttcg aggcgtcggg
     3181 ctggttggcg cggaagcgca ggtacttggc ctcgttgatc ttgccgtact tgtagccggc
     3241 ggcgggctgc tgcatgccca cggtttcatc gccgaggtac atcttgccgg ccacggcggc
     3301 tgaggggggc acccacatgt cgccgtcctc gtactggttg gcgctgcggg cgaggaggta
     3361 gttggcgaag agcgagacgt actgcttgca ctcgtcgctg ctcatgaggc cgtccatgct
     3421 cggatcttcg agcatctcca tgatgtcctc gaccgactcg agatcgggca ggtcggtgac
     3481 gacgtgcacg cggttgggca ggccgatctg gcgggcaagg gtgtcgatgt tctggacgct
     3541 gccgaggtag ccgggaacca cgcacatcga gtagcagcgt ttgatgcccc actcgcggaa
     3601 ctcggccgcg atggccttgc tcagctgttc gaacttctcc ttgtcgtcgg ggtccatgag
     3661 ttcctcggcg ctcacgttgg cgaaccaggc gttgaccttg gcgtccggtt cggcctgggc
     3721 gttggcgaag aacttatcga tggcccggta gccggtttcc aggtcgcggg ccgccttgtg
     3781 gaccttcttc atgttcttct cgagattgct ctcgagctgg gcggctttct cgttgagttt
     3841 cttgcggagg tcgtcgatgc tgccgccctc ggcgagcatt tcgatccaca gtttgagccg
     3901 ctcggcgagc agccggcgct gccgggcgtg gtccggcttg gtgaggaact ctttgcgttg
     3961 gaattctttt ttggggtcga gccactcgac gccgcggacc aggtcgccgt cggccgtctt
     4021 gaacgacggc aaaagcccgg cgaacttctt gaagtcgccg aaagcgtcgc ccaggatgcc
     4081 tgcgaactcc tcgtcggaga gcagctcttt cgctttggcg ggtttcgcgt cggcctgcgg
     4141 accggaggag gccgcccgtt tttcttcttc gcccattacc ttctacctcg ctctcattcg
     4201 ctcaacaatt ccacgtaata tttggccgct ttcagcaggg cttcccgggt ctggggatcc
     4261 tccagcgttt tcttgagggc ggtgttcttc ttgagctgtt tgtccagatc gctgaggatt
     4321 tccttgctga agtagacttt ctgcaggtac tcgttctgtt cgatgagttc tttggactgg
     4381 aagtccttca tggcgcggat tttgaactcg gccggcgccg actggccgtc gagggtctcg
     4441 acctcagcgg agaaggaggg ctggaacttt tcgaacacct gcttgagatt gttgcactcc
     4501 accggctcga cgccggccgc ctcgtcgttg ttgagtttgg ccacatagag cgtcttgttc
     4561 gagggaagca gctcgaccgg gatcgagtcg tccttcacct ttcgttcgcg ggctccgagc
     4621 caaaagttgt tggccattcc gatatcctcc gaatctgagt attgttatcc gcgggattcc
     4681 ccggttcggg tgaacacgta ctgatcgtcc tgtacggcga tgacgacggc gtcgccgggc
     4741 gcgagggtgc cggtgatgat ctcgaccgag aggcggttag tgatgtcgcg ctcgatgacg
     4801 cgctggatcg gccgggcgcc cagctcgaaa gtgtagccgt cgcgcgcgag cttggcccgc
     4861 gcctcggggc tcagggagac ggcgatgttc tgttcggcga gcatgcgggc gagctgggca
     4921 aattccagtc ccgcaatggt ctccacctgg gccggggtga acgtatgata gatgatgatg
     4981 tcattgaggc ggttgagaaa ctccggacgg aacttggtga agaggaattc gcggacctgg
     5041 tccatgtcga cctcccgccc ttcgcggtcg gcgtcgagta tcaggtcggc ggcgtagttg
     5101 gaggtgagga cgacgatgca gttggagaaa tccaccgtcc ggttctgccc gtcggtcagc
     5161 cgcccgtcgt caagaagctg caggaggatg ttaaagacgt cggggtgggc cttttcgacc
     5221 tcgtcgaaga gcacgatcga gaagggtttg cgccgcaccg cctcggtcag cacgccgcct
     5281 tcctcatacc cgacatagcc ggggggcgcg ccgatcattt tggcgaccga gtgtttttcc
     5341 atgaactcgg acatgtcgag ccggaccatg gcgttcttgt cgtcgaacag gaattcggcg
     5401 agcagtttgg gcaggtaggt cttcccggtg ccggtggggc cgaggaagag gaacgatccg
     5461 accgggcggt tggccagttt cagtccggcc cgcgccttgc ggatcgcgtt ggccacgctc
     5521 gtgatggcct tctcctgtcc gacgattttg gaggccaggt gcgcctccat attaatgagc
     5581 cggtccttct cggccgtcat catcttcgac aggggaatcc cggtccgccg ggccacgacc
     5641 gcgccgatgt cggattcgtg gatgaccggc tggaggttcc gcaccacctc ggccagatcg
     5701 cggcagcgtt gctgctggcg ggcgaaggcc tcgcggatat ctttcagggt cagttcgggt
     5761 ctggccatca gccgctcacc tccggcacct ccaggcggtg cccccagtac ccgccgaggg
     5821 cgtccagttg cccggcgaac gtctcgtaga gggcgcgcag tttttgctgg tccccgtcgc
     5881 tcaggccggc cccgtcggcg gccgccagga gcggctcaat gtctttcata gcggcttcca
     5941 gccccggcag gaggcgctcg gccatttctt tcttgacgcc gaattcgctg gccgcttcat
     6001 cgatgatgtc gagggcgatg tcggggaggt ttttctcgct gaggtagcgt ttggcgtact
     6061 tgacggcggc cacgcgcgcc tcggggctga aagtcacgcg gtggtgttcc tcgtactttg
     6121 cggccacgcc gttgacgatc gccaccgcct cgtcgaaggg cggttccccg accttgacct
     6181 tctcgaaccg gcgcaggagc gccttgtctt tttcgaggtg tttggtgtac tcctcttcgg
     6241 tggtcgcgcc gaccaggcgg agctggccgc gggcgagggc gggtttgagg agattggcgg
     6301 cgtccatacc ggcgccgccg cttcccgcac ccatgatggt gtggacttcg tcgatgaaca
     6361 ggatgaggcg gccggccgac ttgatgacct cgccgatgag cgccttgaac cgctcttcga
     6421 attcgccttt gtacttggcg ccggccacga gcccgcccat gtccacttcg agaactttga
     6481 cgccctggag ggccttgggg accgctccgc tcacgaccgc ctgggccagg ccctcgacga
     6541 tcgccgattt gccgaccccg gcgcccccca cgaggacggg ggaatttttg cggcggcgga
     6601 gcagaatctg aatcacctgg cgcagttcct cggcccgccc gatcatcggg tcgtactcgc
     6661 cggccgccgc ccggccggtg agatcggcgc agtagcggga gaccgcggcg accgtcctgg
     6721 gctcctcggc cggggcagcc gatccctcgc cccggggcgg ggagacagcg atcgcctcga
     6781 ccgaccggct ctccgccatg gcctggtaca aatcctcctt ggtgaactcc agttgttcgc
     6841 ggaggtaggg cgggagagcg gaggcgcggt cgaatatcgc caggaggata tgctcgggct
     6901 cgacgagggc gtcgtagagc tttccctttt cctcgaaagc ctgcgcgaga accgcctgca
     6961 ccgccggcga ggcgctgagg ctgtcccggg cagcggcccg cgagggctgc tgcttgaggt
     7021 aactctcgac catggcggcg accgtggcgg gatttttctt gagctggttg aggatcgact
     7081 cgacctcgga accctcgtgg cgcaccaccg ccaccaggag gtgctcgatg ctgatttccg
     7141 gatgtcggaa ttcagcggca acttcccggg ccactttaat tacggcgcgg gaatcggatg
     7201 agtatttcgc tatatccata tgcggactgc cgccgcgtcg ctcggctatg ggtctgcagg
     7261 tgtctgcctc acaagaggat gcgaaccccc tcgcgagttg catcaggaac taaatcttat
     7321 cattccagta tcgcattgtc aagtaatttg tcgcgacgcg gggataccga gcggcgaccc
     7381 ggcgccctcg aaccccacta aggcgacgaa ttgccggctt tcaggctgtc gcggccggcg
     7441 gagccgatcc ggtagtccac gagatcgatg gacatataga gcacgctggt ggagaccaga
     7501 ccgattccct ggcggaactc atacccgacc cagtccacat cgcgctccgg gttgttttcg
     7561 agaaaccagc aggagtcggc gccgtagggg cattgctcgt aagtgaaatc gcccacggtt
     7621 gcactataga cgggttgcag gagc
//
LOCUS       DAOOKS010000010         7247 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_12193_length_7247_cov_3.437152, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000010 DAOOKS010000000
VERSION     DAOOKS010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7247
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12193_length_7247_cov_3.437152"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            352..1080
                     /locus_tag="PK112_00350"
     CDS             352..1080
                     /locus_tag="PK112_00350"
                     /inference="COORDINATES: protein motif:HMM:NF003952.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HPC10532.1"
                     /translation="MAGVVAIIPARLGSTRFPRKVLHLYRGRPLLYYVWREVSRARRI
                     DRVAVATDSREIVAAVREFGGEAVRTSARHRTGSDRAAEAAEKIGGRIIVNVQADNFG
                     LTGAALDRVVARMEQDRTIEFATLAFPIGGAEDLADRNLVKVVASDREGRALWFSRWP
                     IPLVRDAGARYRHLGHIGVYFFRRAALRRYAEWPRTAAERAESLEQLRILEHGGRMLV
                     FETRARTVSVDAPPDVAKLDTLYR"
     gene            1089..2765
                     /locus_tag="PK112_00355"
     CDS             1089..2765
                     /locus_tag="PK112_00355"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008188463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HPC10533.1"
                     /translation="MADKTKYIFVTGGVVSALGKGISSASIGLLLQRRGLTVYNMKLD
                     PYLNVDPGTMNPYQHGEVFVLDDGSETDLDLGHYERFLDRSLTRDHNVTTGQIYHTII
                     TRERRGDYLGATVQVIPHVTEEIKNRIRRLERLPEKPDVVICEIGGTVGDIESLPFME
                     AIRQMGLEQGYDNVMYVHVTLVPYIATAGEFKTKPTQHSVKELRAIGIQPNMLLCRSA
                     KPLDEGLRQKISLFCSVPTKSVIAAEDVSTIYEMPLRFRAQGMDDIICRHFGLETPEP
                     DLGDWEQMVERISYPTHRLKIAICGKYVMLKDAYKSIIEAFVHAGVHNGAEVKLIWVS
                     SEEIKHNGAGKFLHDVDGLLIPGGFGERGVEGKIEAIRYVRENRIPFLGICLGMQCAV
                     IEFARNVCGLEDAHSYEFYRDLKHPVIHLMADQEGVTELGGTMRLGAYPCRLAAGSLA
                     RQAYGADEISERHRHRYELNNAYREMLGSHGLRLSGMSPDGRLVEIIEHPEHPWFVAS
                     QFHPELKSRPTRPHPLFRDFVKAAVDYKVRRNGASGSNNEEEHDLSEAAR"
     gene            2794..3282
                     /locus_tag="PK112_00360"
     CDS             2794..3282
                     /locus_tag="PK112_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10534.1"
                     /translation="MSRAGKARFRPLILLLAAAALAGGCKEDRQALAPPEVYTGMMVM
                     EPDFASGPMEIYPDSTMVTFTVEGTLYELLLEDETDFHLCHTRGTVRSFGTNALTLAP
                     TGTFGAGNCDSICVPRGELPTVFRGDSLFIGPKDIAYEIHINNTTVTDIMRYTFRLRR
                     VQ"
     gene            complement(3309..4421)
                     /locus_tag="PK112_00365"
     CDS             complement(3309..4421)
                     /locus_tag="PK112_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10535.1"
                     /translation="MNFARLRSKSFLVPTAVTFLFLAVVLALLGTGERKYAPLARTLI
                     QWDGRHYLSIAETGYEKFPCPEDPSLVCGNVGWFPLFPLAARVVSVALGPLGLGMAWS
                     MLITGWLCLWAAMLLLYRLFARRFDHTVAAGSLAALALFPTSFYYLTAFPYALYLLLA
                     AAVFLLVEKERYFAAAAPAGLLAITYPTGVAIILPLLWVLVSRWRGLTPRDRAGLRTA
                     SAAVVLALILYALYYRLAFGDFFLYVHFQSKPYYAHGATVPLLPILDAVVHLPGAHPV
                     RVMLIFVAAALALFSTRRIPAAWQVFLWGVLLFTPAAGTTACYYRHVVVAFPLFAMVG
                     LSLRSRRRPLAVLYAAAALALMLGVFLDAYKRGVLM"
     gene            4559..6280
                     /locus_tag="PK112_00370"
     CDS             4559..6280
                     /locus_tag="PK112_00370"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HPC10536.1"
                     /translation="MDLRKPKVATVGLSAVLALYYALPFLLKLTWWGVRDWDLFTTIA
                     AVPVGTVLHYGQFPFWNPYLGGGNILFHHPEVAVLSPFVLLYFLCGPVVGLKLQVLIA
                     YFLGFWGSLRLFRTLGMPRGSTVVAAVAYFGSVHFALHFAEGHMPFTHYAFLPWFVHF
                     VLKGAEDRRNLLGAVVALALMILGNGAAVPLLYTLLATALLLALRAAQRRSPAEARVF
                     LLAVGLALALAAVKVLPMIAYLAQNRWTGDPAEAIPWSALPAMFFGLKHSLFVQNFAG
                     QKWAWHEYGVYVSPLLVAAGAYAAVARFRRHWPWVVFIAFFLLLGLGNFAPWSPWALL
                     TGLPGFESLRATGRAMHFVILGAAVLGGLGLGCWEDRARAAARGGWLRGAVIAAGLLI
                     AATNLALAWPIMSEGFRKPPKHVDRSPEFRQVVDAEPHAFENYLANRGSLVSPWLSAY
                     HPSRGLVGPGIVYSELFSGGEAKVYVRDYTPNRITYTLNGGGHGGELVIGMGYDPGWR
                     ATDGRTITENQGLIAFPVTPGRQTVVLVYRTPYFSHGAVISLLTLGGMVLWRRRLFGR
                     PGRGANL"
     gene            6383..>7247
                     /locus_tag="PK112_00375"
     CDS             6383..>7247
                     /locus_tag="PK112_00375"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HPC10537.1"
                     /translation="MRLVSTILAVLMVTAAAAIAQPDRADSVGAGGYRFFDMPIDPER
                     YLLRPDERLQVTFLGTALAPLTVRVNPEGRIVHPTLGSFDLRGATLAEARVALVKALS
                     SLYQADSIDISVLAPRRVAIQVSGAVQSPGLYTVYTSQRVSEVIDSAGGLSPSGSRRL
                     IRLLGGPREIRVDLDRALLGDERWNPPLYAGYTVIVPAKSARVVNVVGAVDRPRDIEI
                     VDGESVAEILPLVGPFRSSADTLAVQIIRGSERLPAAGVPLQAEDIVFVPTLSRLAEG
                     APVTVYGAVARP"
BASE COUNT         1184 a         2250 c         2603 g         1210 t
ORIGIN      
        1 ccggggagga ggaactttag ggcagggtgg agcccggggg acgggcgcgg gcggcaaaaa
       61 ccttgacagg ccgcgggcgc cgccgtatca tcgctcatgc cgctcgacag catccagatc
      121 ggccgcggag cacggccccc ccgaccgaaa actcccactc ccctgaccat agaatccccg
      181 tttcagcggc tcggagcgcg gtggtcgcgc catgccttgg gggcaggtcg tcatgccgct
      241 aatcccttgt tgcagaatat gataaacgtc ggtagcccgg gtctcacgat ggacgtcggc
      301 cagggagatt ggcggccgga ggaaccgggt gatccggccg gggaggatca aatggcgggg
      361 gtagtcgcga tcattcctgc gcgcctgggg tcgacccggt ttcctcggaa ggtactccac
      421 ctttaccgcg ggcggccgct gttgtactat gtgtggcgcg aggtcagccg ggcccggcgg
      481 atcgaccggg tggcggtggc gaccgactcc cgggagatcg tagcggcggt cagggaattc
      541 ggaggcgaag cggtgcgtac gtcggcccgg caccggaccg gatcagaccg ggcggcggaa
      601 gcggccgaga agatcggcgg gcggatcatt gtgaatgtgc aggcggacaa cttcggcctc
      661 accggcgcgg cgctcgaccg cgtggtcgcg cggatggagc aggatcggac gatcgagttc
      721 gcgacactgg catttccgat cggcggggcg gaggatcttg cggaccggaa tcttgttaaa
      781 gtagtggcgt cggaccggga ggggagggcg ctgtggtttt cgcggtggcc gattccgctg
      841 gtgcgcgacg ccggggcgcg ctaccgccac ctcgggcata tcggagtgta cttcttccgg
      901 cgggccgccc tccggcgcta tgcggagtgg ccccggacgg cggccgaacg ggccgagtcg
      961 ctcgagcagt tgcggattct ggagcacggc ggccggatgc tggtgtttga gacgcgcgcg
     1021 cggacggtgt cggtggacgc tccgccggat gtggcaaagt tggatacgct atacaggtag
     1081 aggatgcgat ggcggacaag accaaataca ttttcgtgac cggcggcgtg gtctcggccc
     1141 tgggcaaggg gatctcgtcg gcctcgatcg ggctgttgct gcagcggcgg ggtctgacgg
     1201 tgtacaacat gaagctggac ccgtacctca atgtggaccc ggggacgatg aacccgtacc
     1261 agcacgggga ggtgttcgtg ctcgacgacg gttcggagac ggatctcgac ctggggcact
     1321 acgagcggtt tctcgaccgc tcgctcaccc gcgaccacaa cgtcaccacc gggcagatct
     1381 accacacgat catcacgcgc gagcggcggg gggattacct cggggcgacc gtgcaggtca
     1441 tcccgcacgt gacggaggaa atcaagaacc gcatccggcg cctggaacgg cttccggaga
     1501 agccggacgt ggtgatctgt gagatcggcg ggacggtggg ggacatcgag tcgctcccct
     1561 tcatggaggc gatccgccag atggggctgg agcaggggta cgacaacgtc atgtacgtgc
     1621 acgtcacgct cgtgccgtac atcgcgacgg ccggcgagtt caagaccaag ccgacgcagc
     1681 attcggtgaa agagctgcgg gccatcggga ttcagcccaa catgctcctg tgccggtcgg
     1741 ccaagccgct cgacgaaggg ctgcgccaga agatcagcct cttctgctcc gtgccgacca
     1801 aatcggtcat cgccgccgag gacgtctcga cgatttacga gatgccgctg cggttccggg
     1861 cgcagggaat ggacgatatc atctgccgcc atttcgggct ggagacgccg gagccggacc
     1921 tgggcgactg ggagcagatg gtggagcgga tcagctaccc gacccatcgg ctcaaaatcg
     1981 cgatctgcgg gaaatacgtg atgctcaagg acgcctacaa atcgatcatc gaggcgttcg
     2041 tccacgcggg tgtccacaac ggggccgagg tcaagctcat ctgggtgagt tccgaagaga
     2101 tcaaacacaa cggcgccggc aagttcctcc acgacgtcga cggcctgctg atccccggcg
     2161 ggttcgggga gcgcggggtc gaggggaaga tcgaggctat ccgctacgtg cgcgagaaca
     2221 gaatcccgtt tttgggcatc tgcctcggca tgcagtgcgc cgtcatcgag ttcgcccgga
     2281 acgtctgcgg cctcgaggac gcccacagct acgaattcta ccgcgacctg aaacacccgg
     2341 tgattcacct catggccgac caggaagggg tgaccgagct gggcgggacg atgcggctgg
     2401 gggcgtaccc gtgccgactg gccgcgggct cgctcgcccg ccaggcctac ggcgcagacg
     2461 aaatctccga acgccaccgt caccgctacg aactgaacaa cgcctaccgg gagatgctgg
     2521 ggtcgcacgg cctgcggctg agcgggatgt cgcccgacgg gcggctggtg gagatcatcg
     2581 agcatcccga gcatccctgg tttgtcgcct cgcagttcca cccggagctg aaatcgcggc
     2641 cgacccggcc gcacccgctg ttccgcgact ttgtcaaggc cgcggtggac tacaaggtgc
     2701 ggcgcaacgg cgcctccgga tcgaacaacg aggaagagca cgacctgtcc gaggcggcgc
     2761 gctgacgacg cgcgggtggg aggaggaccg aggatgtcga gagcggggaa ggcgcgtttc
     2821 cggccattga tcctgctgct ggcggcggcg gccctggcgg gggggtgcaa agaggaccgg
     2881 caggcgctcg cgcctccgga agtgtacacg gggatgatgg tgatggagcc ggatttcgcc
     2941 tcgggtccca tggagatcta cccggactcc accatggtga cgttcacggt cgagggcaca
     3001 ctctacgagc tgctgctgga ggacgagacg gattttcacc tctgccacac gcgggggacg
     3061 gtgcggtcgt tcgggacgaa cgcgctcacg ctggctccca cggggacttt cggcgcgggc
     3121 aactgcgatt cgatctgcgt cccgcgaggg gaactcccga ccgtgttccg gggggacagc
     3181 ctcttcatcg ggccgaaaga catcgcctac gagattcaca tcaacaacac caccgtcacc
     3241 gacatcatgc gctacacgtt ccggctgagg cgggtgcagt aggggcggcg cggggttcgg
     3301 cccggcggtc acatcaggac gccgcgtttg taggcgtcga ggaagacccc cagcatgagc
     3361 gccagggcgg cggcggcgta gaggaccgcc agcgggcggc ggcgcgagcg cagcgacaac
     3421 ccgaccatcg caaacagcgg aaacgcgacc acgacgtggc gatagtagca ggcagtcgtc
     3481 cccgcggccg gagtgaacag cagcacgccc cacaggaaaa cctgccaggc ggccgggatc
     3541 cggcgcgtgg agaagagcgc gagcgcggcg gcgacgaaaa tcagcatgac acggaccggg
     3601 tgggcgccgg gcaggtgcac gaccgcatcg aggatcggga gcagcggtac ggtcgccccg
     3661 tgggcgtagt acggcttgga ctgaaagtgc acatagagga agaagtcgcc gaaagccagc
     3721 cggtagtaga gcgcgtagag gatcagggcg agcacgaccg cggcggaggc ggttcgcagg
     3781 ccggcccggt cgcggggcgt aagccctcgc cagcgtgaga cgagaaccca cagcaggggg
     3841 aggatgatcg cgaccccggt ggggtaggtg atcgccagca gtccggccgg cgccgccgcc
     3901 gcgaagtagc gctccttctc cacaaggagg aagacggcgg cggccagcag caggtacagg
     3961 gcatagggga aggcggtgag gtagtagaac gaggtgggga agagcgcgag ggcggccagc
     4021 gacccggcgg cgacggtgtg gtcgaaacgc cgggcgaaca ggcggtacag caggagcatc
     4081 gccgcccaga ggcagagcca cccggtgatc agcatcgacc aggccatgcc gaggccgagc
     4141 gggccgagcg caacactcac cacccgcgcc gccagcggaa acagcgggaa ccacccgaca
     4201 ttgccgcaca cgagcgaggg atcctccggg cagggaaatt tctcgtaccc ggtctccgct
     4261 atcgacagat agtgccggcc gtcccactgg atcagcgtgc gcgccagcgg cgcgtacttg
     4321 cgctcccccg tcccgagaag cgcgaggacg accgcgagga agaggaaagt cacggcggtc
     4381 gggacgagga aacttttact gcgcaggcgg gcgaaattca ttaagttgtt ccgttcgcgc
     4441 gacccggcgg ggccggcgcg gatctccggc cgcggatcgc ggctcttcgg actctcccgg
     4501 cgcgaccgac gccggcggcg ccggccgata ccggacagga tggaaagtag catcccggat
     4561 ggacctgcga aagcccaaag ttgcgactgt tggtctctcg gccgtcctgg cgttgtacta
     4621 cgcgctgcca ttccttttga agctgacgtg gtggggggtg cgggactggg acttgttcac
     4681 caccattgcc gctgttccgg tggggaccgt ccttcactac ggccagttcc ccttctggaa
     4741 tccctatctc ggcgggggga atatcctctt tcatcatccc gaggtcgcgg ttctgtcgcc
     4801 ctttgtgctg ctgtattttc tgtgcgggcc ggtggtcggg ctgaaactcc aggtgctgat
     4861 cgcgtacttc ctcggatttt gggggagcct gcggctcttt cgcacgctgg ggatgccccg
     4921 gggcagcact gtcgtggccg ccgtcgcgta tttcgggtcg gtgcatttcg cgctccattt
     4981 tgccgagggg cacatgccgt tcacgcacta cgcttttctg ccgtggtttg tgcactttgt
     5041 cctcaaggga gcggaagatc gccgcaacct cttgggggcg gtcgtcgcat tggcgctcat
     5101 gatcctcggc aacggcgcgg ccgtgccgct tctgtacacg ctgctggcga ccgctctgct
     5161 gctggcgttg cgggcggcgc agcggcgctc gccggccgag gcccgggtgt ttctcctggc
     5221 ggtcggcctc gcgctggccc tcgcggcggt gaaggttctc ccgatgatcg cgtaccttgc
     5281 ccagaaccgc tggacgggcg acccggcgga ggcgatcccg tggtcggcgc tgccggcgat
     5341 gttttttggt ttgaagcact cgctgttcgt gcagaatttc gccggccaga aatgggcctg
     5401 gcatgaatac ggcgtctacg tgtcgccgct gcttgtggcc gcgggagcgt acgccgccgt
     5461 ggcgcggttc cggcggcatt ggccctgggt ggtgttcatt gcctttttcc tgctcctcgg
     5521 actgggtaac ttcgctccgt ggtcgccctg ggcgctgctc accggtttgc ccgggttcga
     5581 gtcgctgcgc gccaccggcc gcgccatgca cttcgtcatc ctcggagcgg ctgtgctcgg
     5641 cggactgggc ctgggctgtt gggaggaccg ggcccgggcg gcggcccggg gagggtggct
     5701 gcggggagcc gtgatcgcgg ccgggctgct catcgcggcc accaacctgg cgctggcctg
     5761 gccgatcatg tccgaggggt ttcggaagcc gccgaaacac gtggatcgat ccccggagtt
     5821 ccgccaggtg gtcgacgccg agccgcacgc gttcgaaaac tacctcgcca accggggctc
     5881 gctcgtgtcg ccgtggctct cggcctacca tcccagccgg gggctggtcg gccccggcat
     5941 agtgtattcg gagctattct cgggcgggga ggcgaaagtc tacgttcggg actacacccc
     6001 gaatcgaatc acttacactc tcaacggcgg cggccacggc ggggaactgg tgatcgggat
     6061 gggctacgat ccgggctggc gcgcgaccga cggccggaca atcacggaga atcagggact
     6121 gatcgcattc ccggtcacgc cgggacggca gacggttgtc ctggtctacc gaacgcccta
     6181 cttctctcac ggggcggtca tttcgctttt gacgctgggc gggatggttc tgtggcgccg
     6241 gcgcctcttc ggccgcccgg ggcgcggggc gaatctgtag attcggatag ccgttgactt
     6301 cgccttgggc gtgggttatc cttggcggat gcagcgacgg ggttaaccct gcgctcacac
     6361 cttgtgcgga gtgttcggaa tcatgcgtct tgtgtccacc atactggcgg tgctgatggt
     6421 gacggcggcg gctgcgatcg cccagcccga ccgggccgat tccgtcggtg ccggcggcta
     6481 ccgctttttc gacatgccca tcgacccgga gcgctatctg ctgcggccgg atgaacggct
     6541 gcaggtgact tttctcggga cggcgctggc tccgctgaca gtgcgggtca atccggaggg
     6601 gagaatcgtg catcccacgc tcgggtcgtt cgatctgagg ggcgcgacgc tcgcggaggc
     6661 ccgggtggcg ctcgtgaaag cgctctcgtc cctgtaccag gccgactcga tcgacatctc
     6721 agttctcgcg ccccgccgcg tggctatcca ggtcagcggc gccgtgcagt cgccggggct
     6781 ctacacggtc tatacgtcgc agcgcgtctc ggaggtgatc gactcggccg gcggccttag
     6841 tccgagcggc tcgcggcggc tgatccggct cctgggcggt ccccgggaga tccgcgtcga
     6901 cctggaccgc gcgctcctgg gcgacgagcg gtggaatccg ccgctctacg ccgggtacac
     6961 ggtgatcgtg ccggcgaaat ccgcccgcgt cgtcaacgtc gtcggagcgg tggaccgtcc
     7021 ccgcgatatc gagatcgtcg acggagagtc ggtcgccgag attctccccc tcgtcgggcc
     7081 cttccgctct tcggccgaca ccttggcggt gcagatcatc cgcggttcgg aacgcctgcc
     7141 ggcggccggt gtgccgctcc aggcggagga cattgttttt gtcccgacgc tctcccggct
     7201 ggccgagggc gcgccggtga ccgtgtacgg agcggtcgcg aggccgg
//
LOCUS       DAOOKS010000011         6723 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_13521_length_6723_cov_3.402969, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000011 DAOOKS010000000
VERSION     DAOOKS010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6723
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13521_length_6723_cov_3.402969"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1276
                     /locus_tag="PK112_00380"
     CDS             <1..1276
                     /locus_tag="PK112_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10538.1"
                     /translation="LYNLDAYFTTGRLPAAVEPITEAAPAGGGVHNADASYGYAVDMV
                     GEKTYLVLNRLFAEDVTVHAAEKPFRLEGRDYGAGALVLRRRGNPDNLPDIVARLAAE
                     VGIDIRGISTGYSEIGSKLGAGTFRLLRQPRVALAVGMPLSSGTSGSLWFTIDRQLGM
                     PHSLVSFDRMAWSDLSSYNVLIIPDSWGGLGDLLGGGIPGKIAKWVGDGGTLILIGSS
                     GDWAADTAGSLSAVRLRSQVLSRLGDYDAALQREIAADAPVVDTMALWHPEKVSAAKE
                     EDKGEREGRATGSDEEADRWARRFHPRGVILQADLNPEHYLAFGLGRRLPVFVWADDC
                     WLAKSPVTAVARLTPDEMKLRLAGLLWPEARRRWAGSAYITHERQGQGQIIMFAGDPT
                     FRAYTYGTRQLLLNAVLYGPGFAGGFESYQQK"
     gene            1455..3410
                     /locus_tag="PK112_00385"
     CDS             1455..3410
                     /locus_tag="PK112_00385"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HPC10539.1"
                     /translation="MKAGLLLVLVAVVVAAAGSALAADPPIPANYPISSPYYELQNEE
                     QIWISPLDSTRVVAVWRDFRLGYRQVAIGIAFGSPDFWVDWLIPPTQQVGEQQSDPTL
                     TVDRNGTFYISVLDYVPAYQFTDSSFISFLKSTDNGMTWSGPVTVEGTSGPYFEDKQF
                     ITVDRTTGPHSGNVYAAWARFPNPTRIMFARSTDGAAGFDPPVIVGQPWDFTDCGWTD
                     VDAGQFANPLVGADGSVYVFWIGVWADPDCNFSYALHCVKSTDGGATFGPPQFIRTTF
                     GNWGQVDGFVDVYNQPVVAADLSAFPHGGNLYVAYANMDTVGSFFDYEIDFLRSLDGG
                     ATWSEPIRINDGLGGDTDSTDQFHPWLVINDEGTLACIWYDQRTDPANHYLFDVYAAY
                     SFDGGASFTRNHRVSEVSINPSSLKKSDRDRPGPDAPAPVAKSALAPMAGLIAEYIGV
                     SIRNNYLHAAWTDTRNGNQDVYGANWEIPFLAPRLISPPDSAVALGPTDDTVALRWST
                     CWRENDDHYTVQIAADAGFSTILAVLDVSAADTVVDAAAFPALSAYHWRVRAHRYALG
                     DATDWSDGRVVRRAATCCVLRGDFNDDGAVKVSDLTALINYLFRGGPAPACAEHGDAN
                     ADGAIKVSDLTLLIDYLFRSGPLPAAC"
     gene            4067..4810
                     /locus_tag="PK112_00390"
     CDS             4067..4810
                     /locus_tag="PK112_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPC10540.1"
                     /translation="MQHRTLHFHQFHGRLRRPGETPVDRGQPLVGVGAYGETKIEGEK
                     ICREFRRDGLCITIIRPKTFIGTARLGVFQILYDWVDSGKRIPIIGRGENLYQLLEVS
                     DLVEAIWLAGAAEPEQTNDVFNVGAQHFEKVKTDVGALCEFAGTGARVQPTPAKLVKG
                     TLAFFEFLRISPLYKWIYGTADKDSYVSTRKIEERLGWKSSFSNQDALISSHRWYLEH
                     KEELKHLKSGVTHRVGWNQGVLKLIKKFM"
     gene            complement(4871..6238)
                     /locus_tag="PK112_00395"
     CDS             complement(4871..6238)
                     /locus_tag="PK112_00395"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HPC10541.1"
                     /translation="MTENRKTLVFVGAAAVLTALAFLLAPSTPTPDEFADQGTPFFPD
                     FTDPNTATTLEVVEFDEATGSTKPFKVTFENGRWVIPSHHNYPADAASQLAQTAAGVI
                     EIARDDFRSDNAADYEALGVVDPLGEGAALSGRGKRVTIRGRNGEVLADLIFGKKVEG
                     RDGFRYVRHPEEKRVYAAKVNIDISTRFQDWIDPDLLQLTRSDIERIEVNDYSINERT
                     RSVDRRGQVVLTKGEDGGWKMAGLPSGQTVDSATVAAMLATLDSLKIVGVRPKPEGLS
                     ASLRQDEQQVSMSQADLLSLQGKGFYLSRDGQLMSNEGELLVHTRDGVAYTLRFGEVV
                     YGSGLEVTAGTEDDQSPEPQGNAQNQYLFVTAEFDPKRLPEPKRPSDTSFVGKPDSLL
                     SDADRQQRSLYNAWRAWQGNAERGRKKAQELNTRFADWYYVISHDSFRKLHKSRGDLV
                     AAGKD"
     gene            complement(6241..>6723)
                     /locus_tag="PK112_00400"
     CDS             complement(6241..>6723)
                     /locus_tag="PK112_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HPC10542.1"
                     /translation="VGDSSFVALRNKRVAHRTLETVEAQTQEFVRERLAREKEAEEEA
                     QAALAEAQQRLTERVNEVRNRTDLDAQTKQIMAQNLQEVENRRFEVLKANIEAQKQAQ
                     VAAAKEDVEGAIRGIQTRIRTLAVLLPPIPVFAFGVFIFVRRRRREQEGAAAAQRLRS
                     "
BASE COUNT         1091 a         2398 c         2047 g         1187 t
ORIGIN      
        1 cctgtacaat ctggacgcct acttcacgac cggccgtctc ccggcagcgg tcgagccgat
       61 caccgaggcc gcgcccgccg gcgggggggt ccacaatgcc gacgcctcct acggctacgc
      121 cgtcgacatg gtcggcgaga agacctacct tgtgctcaat cgcctgtttg ctgaagatgt
      181 gaccgtccac gcggccgaga aaccgtttcg cctggagggg cgcgattacg gggcgggagc
      241 gctcgtgctt cgccggcgcg gcaatccgga caacctcccc gacatcgtcg cccgcctcgc
      301 ggccgaggtg ggaattgaca tccgcggcat cagcaccggg tactccgaga tcggctcgaa
      361 gcttggggcg ggcactttcc gtctgctccg ccagccccgg gtggcgctcg ccgtgggcat
      421 gccgctgagc tccggcacca gcggctcact ctggttcacc atcgacaggc aactggggat
      481 gccccattcg ctggtgtcgt tcgaccggat ggcctggtcc gacctgtcgt cctacaacgt
      541 gctcatcatt cccgattcgt ggggcggact cggcgatctc ctcggcggag gaattcccgg
      601 caagatcgcg aagtgggtgg gcgacggcgg gacgctcatc ctcataggct cgagcggcga
      661 ctgggccgcc gacaccgccg gcagcctctc cgcggtgcgc ctccgcagcc aggtgctgtc
      721 gaggctgggc gactacgatg ccgccctgca gcgggaaatc gccgccgacg cgccggtcgt
      781 cgacacgatg gcgctctggc atccggagaa agtgtccgcc gccaaagaag aggacaaggg
      841 cgagcgggag ggccgggcga cgggatcgga cgaggaagcc gaccgctggg cgcgccgctt
      901 ccacccgcgc ggcgtaatcc tgcaggccga cctcaatccc gaacactacc tcgccttcgg
      961 actcggccgg cgcctgccgg tgttcgtctg ggccgatgac tgctggctgg ccaaatcccc
     1021 ggtcaccgct gtcgcccggc tcacccccga cgagatgaaa ctgcggttgg ccggtctgct
     1081 ctggccggaa gcccgccgcc gctgggccgg cagcgcgtac attactcacg aacgccaggg
     1141 ccagggacag atcatcatgt tcgccggcga ccccacgttc cgggcttaca cctacggcac
     1201 tcgacaactc ctcctgaacg ccgtcctcta cggccccgga ttcgccggcg ggttcgagtc
     1261 gtaccagcag aagtgaccgt tcgacgatgg tatcttgcgg gcggggccgg tacggctccg
     1321 cccgcttctt gtgcctgacg cccccgcacc cgcgggcggc agaatcgact tgcgtccgca
     1381 cgtagaccgg actatattag tcctatatac tatcccgatg tcatcgaagc ccgcccgctc
     1441 aggaaaggat cactatgaag gctggattac tgctggtctt ggtggcggtt gtggtggcgg
     1501 cggccggatc cgcactcgcg gccgatcccc ccatcccggc caactacccg atttcctcgc
     1561 cctactatga gctgcagaac gaggaacaga tctggatctc cccgctcgac agcacgagag
     1621 tggtggcggt ctggcgcgac ttccgcctcg gctaccgcca ggtcgcgatc ggcatcgcat
     1681 tcggctcccc cgacttctgg gtcgactggc tgatcccgcc gacccagcag gtgggcgaac
     1741 agcaatccga ccccaccctg acggtggacc gcaacggcac gttctatatc agcgtcctcg
     1801 actacgtgcc cgcctaccag tttaccgact cttccttcat cagcttcctc aaatccactg
     1861 acaacgggat gacgtggagc ggaccggtga ccgtcgaggg aacgtcggga ccatatttcg
     1921 aggacaagca gttcattacg gtcgatcgca ccaccggccc ccactccgga aacgtctacg
     1981 cggcctgggc ccgctttccc aacccgaccc ggatcatgtt cgcgcgctcg accgacggcg
     2041 ccgccggttt cgatcccccc gtcatcgtcg gccagccctg ggatttcacc gactgcggct
     2101 ggacggatgt cgacgccggt cagtttgcca acccgctggt gggagcggac ggcagcgtct
     2161 atgtgttctg gattggcgtg tgggccgacc ccgactgcaa cttctcctat gcgctccatt
     2221 gcgtgaagtc caccgacggc ggtgcgacgt tcggcccgcc gcagtttatc cgcaccacct
     2281 tcggcaactg gggccaggtc gacgggttcg tcgacgtcta caaccagccg gtggtggccg
     2341 ccgacctctc cgccttcccc cacggcggca acctctatgt cgcctacgcg aacatggata
     2401 ccgtcggatc gtttttcgac tacgagatcg acttcctccg atcgctcgac ggcggcgcaa
     2461 cctggtccga accgatccgc atcaacgacg gcctgggggg agacaccgac agcactgacc
     2521 agtttcaccc gtggctggtg attaatgacg agggcacgct cgcctgtatc tggtacgacc
     2581 agcgcaccga tccggccaat cactaccttt tcgatgtgta tgcggcgtat tcgttcgacg
     2641 gcggcgcatc cttcacccgc aaccaccgcg tgtcggaagt ctccatcaat ccgagttcgc
     2701 tcaaaaaatc ggaccgcgac cgcccgggtc cggacgctcc cgccccggtc gcaaaatccg
     2761 cgctcgctcc catggccggc ctcatcgccg aatacatcgg cgtcagtatt cggaacaact
     2821 acctccatgc cgcctggacc gacacgcgca acggcaacca ggacgtctac ggcgccaact
     2881 gggagattcc gtttttggct ccccggctga tcagccctcc ggattccgcg gtcgcactcg
     2941 gcccgaccga tgataccgtc gctctccgct ggtcgacgtg ctggagggag aacgacgacc
     3001 actacacggt gcagatcgcc gccgatgcag gcttcagcac catcctggcc gtcctggacg
     3061 tttccgccgc cgacaccgtc gtcgacgccg ccgccttccc ggcgctctcc gcctaccact
     3121 ggcgcgttcg ggcgcatcgc tacgcgcttg gcgatgcgac cgattggtcg gacgggcggg
     3181 tggttcgccg cgcggccacc tgctgtgtgc tccgcggcga tttcaacgac gacggagccg
     3241 tgaaagtctc ggacctgacc gccctgatca actacctgtt tcgcggcggt cccgctccgg
     3301 cgtgcgcgga acacggcgac gcgaacgccg acggagcaat caaggtgtcg gatctgactc
     3361 tcctgatcga ctacctgttc cgctccggac cgctcccggc cgcctgttga gaacaggccg
     3421 gacggacaac acacccggcg ccgcgcgata cgcgccgccc ccccccgcag cgcccggacg
     3481 gggcggcgcc atttccgcct tgccttctcc catctatgga ctatattgcc tcccgctccc
     3541 tcgacggggc gggccgatca ggacacgctg tcccgaacgc cgttccagag tgccggacct
     3601 tgatggagat cgggcctccg agcccgccga aagcgggcgg gagatccgac tcgccgcgtc
     3661 gcgcggagtg cggagcgggt caataccggg gtggtcctga ccccaacaga gagactccgg
     3721 cggcatcccg gagcacacca acaggtggga acatgaaagg caaacgcgta ctgattaccg
     3781 gcggcgccgg ttaccttgga tatcatttgg cgcagaatct cccgcggcgc ggcgtggggt
     3841 ttctcgcgct caacgacatc gcccccttct tcaaagacga ataccctccc gacgcccttc
     3901 tggtcaacca ggatgtccgc gatccggagg gaatgtaccg tctcattcgc gaccaccgca
     3961 tcgaggtgat catccactgc gccgccgcgc tccccctctg gaaccgccag gagatcttct
     4021 cgaccaacat cggcggcctg cgcaccacgc tcgagcaggc ccgcaagtgc aacatcgaac
     4081 gcttcatttt catcagttcc acggccgtct acggcgtccc ggagaaacac ccgttgatcg
     4141 aggacaaccc ctcgtcggcg tcggtgccta tggcgagacg aaaatcgaag gggagaagat
     4201 ctgccgggag ttccgccggg acggcctgtg catcacgatt atccgcccca agacgttcat
     4261 cggcaccgcc cggctcggcg tgtttcagat tctctatgac tgggtcgact ccggcaagcg
     4321 catccccatc atcggccggg gcgagaacct ctaccagttg ctcgaagtga gcgacctcgt
     4381 cgaggcgatc tggctggccg gtgcggccga accggagcag accaacgatg tcttcaacgt
     4441 cggcgcccag cattttgaga aagtcaagac cgacgtcggg gcgctctgtg aattcgccgg
     4501 caccggcgcg cgcgtgcagc cgacccccgc caaactcgtc aaaggcacgc tcgccttctt
     4561 cgagttcctc cgcatctccc cgctctacaa gtggatctac gggaccgccg acaaggattc
     4621 ctacgtgtcc acccggaaaa tcgaagagcg gctcggctgg aagtcgagct tctccaacca
     4681 ggacgccctc atctcctcgc accgctggta cctcgagcac aaggaggaac tcaagcatct
     4741 caagtcgggc gtcacccacc gcgtcggctg gaaccagggc gtgctgaaac tgataaagaa
     4801 gttcatgtag ccggggcggc gcggccggcc ggatccgcgg gtcgggcgcc gctgcgggcg
     4861 cccgcccttc tcaatccttc cctgccgcga ccaggtcccc ccgcgacttg tgcagtttcc
     4921 ggaagctgtc gtgggagatc acatagtacc agtcggcgaa gcgcgtgttc agttcctgcg
     4981 ctttcttccg cccgcgctcc gcgttcccct gccacgcccg ccacgcgttg tagagactcc
     5041 gctgctgccg gtcggcgtcg ctgagcaggc tgtccggctt gccgacaaat gaggtgtccg
     5101 acggccgctt cggctcgggg agccgttttg gatcgaactc cgcggttacg aacaggtact
     5161 ggttctgggc gttgccctgc ggctccggcg actgatcgtc ctcggtcccc gccgtgacct
     5221 ccagccccga tccgtacacc acctcgccga accggagcgt gtacgccacg ccgtcgcgcg
     5281 tgtgcacgag cagctcccct tcgttcgaca tgagctgccc gtcgcgcgag aggtagaacc
     5341 ccttcccctg gagcgacagc aggtcggcct ggctcatcga cacctgttgc tcgtcctggc
     5401 ggagcgaggc cgacagaccc tccggtttgg gccgcacgcc gacaatcttg agcgagtcca
     5461 gggtcgccag catggccgcc acggtcgccg aatccaccgt ctgcccggac ggcagcccgg
     5521 ccatcttcca ccccccgtcc tcgcccttcg tgagcacgac ctgcccgcgc cggtccacgc
     5581 tgcgcgtccg ctcgttgatc gagtagtcgt tcacctcgat ccgctcgata tcgctcctcg
     5641 tgagctggag gaggtccggg tcgatccagt cctggaaccg cgtggagatg tcgatattca
     5701 ctttcgccgc gtaaacccgt ttctcctccg ggtggcggac gtagcggaac ccgtcgcgcc
     5761 cctcgacttt cttgccgaaa atgaggtcgg cgagcacctc gccgttccgg ccgcggatcg
     5821 tcacgcgctt gccgcgcccc gagagcgcag ccccttcgcc gagcgggtcg accacaccga
     5881 gcgcctcgta gtcggcggcg ttgtcggagc ggaagtcatc gcgcgcaatc tcgatcaccc
     5941 ccgccgccgt ctgggcgagc tgcgaggcgg catcggccgg gtagttgtgg tgcgagggga
     6001 tcacccaccg cccgttctcg aacgtcacct tgaacggctt cgtcgacccg gtcgcttcgt
     6061 cgaattccac gacctcgagc gtggtggccg tgttcgggtc ggtgaaatcg gggaagaatg
     6121 gggttccctg gtcggcgaat tcgtcggggg tgggcgtcga cggcgccagc agaaacgcca
     6181 gcgccgtgag caccgccgcc gcgccgacaa acacaagcgt ctttcggttc tccgtcatgg
     6241 ctagctcctc aaccgttgcg cggcggccgc gccttcctgt tcccgccggc gccgccggac
     6301 gaaaatgaac accccgaacg cgaacaccgg gatcggcgga agcagcaccg cgagcgtccg
     6361 gatgcgcgtc tggatgcccc ggatcgcccc ctcgacgtcc tccttggcgg cggcgacctg
     6421 cgcctgcttc tgcgcctcga tgttggcctt cagcacctcg aaccgccggt tctcgacttc
     6481 ctgcaggttc tgcgccatga tctgcttggt ctgcgcatcg agatcggtcc ggttgcgcac
     6541 ctcgttgacc cgttcggtga ggcgctgctg ggcctcggcg agcgccgcct gggcctcctc
     6601 ctcggcttct ttctcgcggg cgagccgctc ccgcacgaac tcctgcgttt gcgcttcaac
     6661 cgtctccagc gtccggtgcg cgacccgctt gttgcgcagc gccacgaacg acgagtcgcc
     6721 gac
//
LOCUS       DAOOKS010000012         6509 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_14149_length_6509_cov_3.429346, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000012 DAOOKS010000000
VERSION     DAOOKS010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6509
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14149_length_6509_cov_3.429346"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1522
                     /locus_tag="PK112_00405"
     CDS             <1..1522
                     /locus_tag="PK112_00405"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HPC10543.1"
                     /translation="LSGDRACVRREIAISGPAARPLAVTLCAVPLPDQAAEIVGIVRE
                     ASADQNRRAHDEFSILAEVAAALSSSLELRQILRIILTGATASQGLGFNRAFLFLFDR
                     DRHELTAHMAVGPTSAEEAGRIWTRLAGMHLSLAQLLDVHPSGSEDPTDRLFECIRGL
                     TIALDNESLLAHICDSGTGVNLEPDAELDDLTAGLLRRTGTTRAALVPLMSKGTCQGL
                     VVADNAITGAPIGDDAVRLLKVLADQAAVAIQRARLYDRERQRARELERTYRRLEQSQ
                     EQVIRAEKMSVMGELTAAVADELRNPLTIIEGFTNLLRESHRSECGAEYLNIIASEVR
                     RADALLNQVIEFASASSRQNQVFDFSVLAANVIARLCGQTEHRPSGLSLSAATERLSV
                     YGNPDQLGNAFYQLFQMIVEETADEARIDVRTERADGRARLSLAFQTRPERTERLLRM
                     LDQIFARQTAALRLQVLVASETIRYHGGDYRLSTTDNGAPLLVLEMPLAGQQVGMP"
     gene            1519..1893
                     /locus_tag="PK112_00410"
     CDS             1519..1893
                     /locus_tag="PK112_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10544.1"
                     /translation="MTKSRTDEFRPIDPAIIERVDELNEEVKVLALNLAIYLARAKGR
                     SATLSRLEPDFIKLVNGTVKVVHELADIINAARNLEAMVNDLATRPPGSDPIEGRLRA
                     ILEQCTRIMDALAEEGRVNTKG"
     gene            2003..2419
                     /locus_tag="PK112_00415"
     CDS             2003..2419
                     /locus_tag="PK112_00415"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPC10545.1"
                     /translation="MLDSDMQKLTRLAQERGEPFRVLIVDDEKWVRDVFRDFCGTTDA
                     FRVDLADSGLEALAKVEATPYDLITVDLIMPEMSGLEVLSEIKKRRPRTPVVVITGNA
                     TDKLVHKAGLLGACRVMYKPVQLEDFISEISSALSR"
     gene            2483..2878
                     /locus_tag="PK112_00420"
     CDS             2483..2878
                     /locus_tag="PK112_00420"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPC10546.1"
                     /translation="MKSAVSILVVDDEAMMRLLLEKILSREGYRVATAPDGETAAALL
                     RSQPCDIVISDMKMPGMNGFDLLKIIKREHPRTGVIIMTAYGDTYTVKDALLLGADEY
                     ITKPFKSYEIALVVERAYWRILAASNSSA"
     gene            2889..4322
                     /locus_tag="PK112_00425"
     CDS             2889..4322
                     /locus_tag="PK112_00425"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HPC10547.1"
                     /translation="MTETSPETRVQRPKPKLVRVVITRDNMSAMMAVSDPEHLGGEVT
                     LDDVREEIVRAGVTHGIDWDAIKRVLDQRLYDVPTKIATGTPPRRGKDAAFEYAFEPE
                     TQHTPEESGDGRIDYRSINFIQNVREGTVLVRKIPPGDGEDGCGVDGRLIPGIRGRDF
                     NFRQGKNTRVEPDGLSLVAAASGAIVFTRGTVSVNDVTRIEGDVDMSIGNINCIGSVR
                     VVGDVRPGFTLNVGGNLEVGGNVADCTIRCEGNILIKGGAYGKGEGMIKADGDVLVKY
                     AEGVNIISGNDVLVGDELLNCQVTAAERIIVRARKGKIIGGESNAGREIRVATAGNDA
                     GTATILRVGYDAELMREYRCTGAEIARIEKDLARIRATLLTMYRLQAEGLLDPQRAEA
                     LGRLEEFVAAAPGELERLADVRYRLEERLREVAGAQIIVQDRLYHGVSAHFGVIYREF
                     TDIHKRCKVTLEEGKVVISEYRPDRDG"
     gene            4462..5502
                     /locus_tag="PK112_00430"
     CDS             4462..5502
                     /locus_tag="PK112_00430"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPC10548.1"
                     /translation="MPESKPSKFDLAVIMPVRNEEKYVGQTLEQLYLQDFPMDRLEVV
                     VADGGSTDRTREIVESYKTRFGALRLLDNPVRRPSSGRNIGIRNSTAPRIVVIDGHVY
                     IPSKTLLRDVVETFDRTGADCLCRPQPLTPPDLNDLQTAIALCRGSALGHKPGSEIYA
                     DYEGEVDPTSSGAMWTRATFDRVGLFDENFDAGEDIELNFRVHRAKLKAWLNPALRVY
                     YYPRATLPALWRQMVRYGLGRFRMSRKHGLWAPTHWLAAAGVVLCIVLFVLGFLSLAV
                     MKLFQIVAALYLLTVIFYSLSLALRHRNLNCLLYGPLIFPTIHFGLGVGFLKGVIESY
                     SPPPAREAGPPQ"
     gene            5539..>6509
                     /locus_tag="PK112_00435"
     CDS             5539..>6509
                     /locus_tag="PK112_00435"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HPC10549.1"
                     /translation="MLTTFSHSALQTFAQCRRKFQFRYLERPEIPKRISAEAYLGTAV
                     HTVLQALYERGADGVELSLEAALEIYERAWREVDRALLAVVTEHMGIDDYIRIGREIL
                     ARHHERYRPFRPGALLGAELHLHFTLPGTPYRLSARIDRLWRRDDGTVEICDYKTGKN
                     LSRPHDPDYFHQMGLYQLAVMANYPQYRDIEAAQYCLRMDEVVAHRFRPDDLELLVEN
                     IRQQILAIHEADRLSQFPTRESSLCNWCEYAALCPAKRHKLLIEAGEEDRDGALRRAY
                     AKATEYIARYAAVKELEAEMAALRADLVRLAGELQMTKFSGEGGEVAV"
BASE COUNT         1171 a         2306 c         2035 g          997 t
ORIGIN      
        1 cctcagcggc gaccgggcct gcgtgcgccg agagattgcg atcagcggcc ccgccgcccg
       61 cccgctggcg gtgacgctgt gcgccgtacc gctgcccgac caggcggcgg aaatcgtggg
      121 catcgtccgc gaggcctccg ccgaccagaa ccgccgcgcc cacgacgagt tctccatcct
      181 ggccgaggtg gccgcggccc tgtcgtcctc gctggagctg cgccagattc tccgcatcat
      241 tctcaccggg gccaccgcct cgcaggggct cggtttcaac cgggcgtttc tcttcctctt
      301 cgaccgcgac cggcacgagc tgaccgccca catggcggtc gggccgacct cggccgagga
      361 agccgggcgc atatggaccc gcctggcggg gatgcacctg tcgctggcgc agctgctcga
      421 tgtgcacccc agcgggtcgg aggacccgac cgaccggctg ttcgaatgca tccgcggtct
      481 caccatcgct ctcgataacg aatcccttct ggcccacatc tgcgattccg gcacgggcgt
      541 gaatctcgag cccgatgccg agctcgacga cctgaccgcc ggtcttctcc ggcgcaccgg
      601 gacgacccgg gccgccctcg tgccgctcat gagcaaaggg acgtgccaag gcctggtggt
      661 ggccgacaac gcgatcacgg gcgcgccgat cggcgacgac gcggtgcggc tgctcaaagt
      721 gctggccgac caggcggccg tggcgatcca gcgcgcccgc ctctacgacc gcgagcgcca
      781 gcgcgcccgc gagctcgaac gcacctaccg ccgccttgaa cagtcccagg agcaggtcat
      841 ccgggctgag aagatgtcgg tcatgggcga gctcaccgcc gccgtggccg atgaactgcg
      901 caacccgctc accattatcg aaggcttcac caacctgctt cgtgagagcc accgcagcga
      961 gtgcggcgcc gagtatctca acattatcgc cagcgaagtg cggcgggccg acgcgctctt
     1021 gaaccaggtg atcgaattcg cctcggcctc cagccgccaa aaccaggtct tcgatttctc
     1081 cgtcctcgcg gccaacgtga tcgcccgtct ctgcggtcag accgaacacc ggccgagcgg
     1141 cctgagcctg tcggcggcca ccgagcggct ctcggtatac ggcaacccgg accagctcgg
     1201 caatgcgttc taccagttgt tccagatgat cgtcgaagag acggcggacg aagcccgcat
     1261 cgacgtgcgc acggagcggg ccgacggccg ggcgcggctc agcctggcgt tccagacgcg
     1321 gccggagcgc accgaacgcc tcctgcgcat gctcgaccag attttcgccc gccaaacggc
     1381 ggccctccgg ctgcaggtgc tggtcgcgag cgagacaatt cgctaccacg gcggcgatta
     1441 ccgactgagc acgaccgaca acggagcccc gctcctggtg ctcgaaatgc ccctcgccgg
     1501 gcagcaagtg gggatgccat gacgaaatcc cgaaccgatg agttccggcc catcgacccg
     1561 gccatcatcg aacgggtcga tgagctgaac gaagaggtga aggtgctggc cctcaacctc
     1621 gccatctacc tcgcccgcgc caaagggcgg tcggcgaccc tcagccgcct ggaacccgac
     1681 ttcatcaagc tggtcaacgg cacggtcaag gtggtgcacg aactggcgga tatcatcaac
     1741 gccgcccgga acctcgaggc gatggtcaac gacctcgcca cccggccgcc ggggagcgac
     1801 ccgatcgagg gccgtctccg cgccatcctg gagcagtgca cccgcatcat ggacgctctg
     1861 gccgaggagg gacgggtcaa cacgaaagga taacgcgcag cgccccttgc ccgatgcggt
     1921 acaagacttg tccggatttg ccgataaacc gggcaaagag cggcgcaaac gacactgcca
     1981 aaccccaacc gggatgatgc cgatgctcga cagcgacatg cagaaactga cccgcctggc
     2041 tcaggaacgc ggcgagccct tccgcgtgct catcgtggac gacgagaagt gggtgcgcga
     2101 cgtcttccgc gatttctgcg gaacgaccga cgctttccgc gtcgacctgg ccgacagcgg
     2161 gctcgaggct ttggcgaaag tcgaggccac cccttacgac ctgatcacgg tcgacctcat
     2221 catgcccgaa atgtcgggcc tggaggttct ctccgagatc aagaagcggc gcccccggac
     2281 gccggtggtc gtgatcacgg gcaacgccac cgacaagctg gtgcacaaag ccggcctgct
     2341 gggcgcctgc cgcgtaatgt acaaaccggt gcagctggaa gatttcattt ccgaaatctc
     2401 gtccgcgctc agccggtgac cgggcgccgg ggagaccggc acccaaaagg cgaacgacaa
     2461 ttgcacggag gacaccaggc ccatgaaaag tgcggtctcg attcttgtag ttgacgatga
     2521 ggccatgatg cggctcctgc tggagaagat cctctcccgc gaagggtacc gagtggcgac
     2581 ggcgccggat ggcgagacgg cggcggccct gctccggagc caaccctgcg acatcgtcat
     2641 ctccgacatg aagatgcccg ggatgaacgg gttcgacctg ctcaagatca tcaagcggga
     2701 acacccccgc actggcgtga tcatcatgac cgcctacggc gacacctaca ccgtcaagga
     2761 tgccctcttg ctgggggccg acgaatacat caccaaaccg ttcaagagct acgaaatcgc
     2821 cctggtggtc gagcgcgcct actggcgcat cctggccgcc tcgaactcct cggcctgaga
     2881 gtgcacccat gaccgagacc tcgccggaaa cccgggtcca gcgtcccaaa ccgaaactcg
     2941 tgcgggtcgt catcacgcgc gacaacatgt ccgccatgat ggccgtgagc gacccggaac
     3001 acctcggcgg ggaggtcacc ctcgatgacg tccgggagga gatcgtccgt gccggcgtga
     3061 cccacggcat cgactgggac gccatcaagc gcgtcctcga ccagcggctc tacgacgtcc
     3121 cgaccaaaat cgccaccggc acgccccccc ggcgcggaaa ggacgccgcc ttcgagtacg
     3181 ccttcgaacc cgagacccag cacacgcccg aggaatccgg cgacgggcgc atcgactacc
     3241 gctcgatcaa tttcatccag aacgtccgcg aggggaccgt gctggtgcgc aagattccgc
     3301 caggcgacgg cgaggacggg tgcggggtgg acggccgtct gatccccggc attcgcgggc
     3361 gcgatttcaa cttccgccag gggaagaaca cgcgcgtcga gccggacggc ctctcgctgg
     3421 tggcggccgc cagcggcgcc atcgtcttca cccgcggcac cgtctccgtc aacgacgtca
     3481 cccgcatcga gggggacgtc gacatgagca tcggcaacat caactgcatc ggctcggtgc
     3541 gcgtcgtcgg cgacgtccgg cccggcttca ccctcaatgt cggcggcaac ctcgaagtcg
     3601 gcggcaacgt cgccgactgc accatccgat gcgagggcaa catcctcatc aaggggggcg
     3661 cctacggcaa gggggagggg atgatcaagg ccgacggcga cgtgctggtc aagtacgccg
     3721 agggggtcaa catcatctcc ggcaacgacg ttctcgtcgg cgacgaactc ctcaactgcc
     3781 aggtcacggc ggccgagcgc atcatcgtcc gcgcccgcaa aggaaaaatc atcggcggcg
     3841 aatccaacgc cggccgcgaa atccgcgtcg ccaccgccgg caacgacgcc ggcaccgcca
     3901 ccatcctccg cgtcggctac gacgccgagc tcatgcgcga gtaccgctgc acgggtgctg
     3961 aaatcgcgcg catcgagaaa gacctcgcgc gcattcgggc cacccttctg accatgtacc
     4021 ggctgcaggc ggagggtctg cttgatcccc agcgggccga ggccctgggc cgactggagg
     4081 aattcgtggc cgccgccccg ggcgagctcg aacgcctcgc cgacgtccgc taccggctcg
     4141 aggagcgcct gcgcgaggtc gcgggcgcgc agatcatcgt ccaggaccgc ctctaccacg
     4201 gtgtctccgc ccatttcggc gtcatctacc gcgaattcac cgacatccac aaacgctgca
     4261 aggtcacgct ggaggagggg aaagtcgtga tcagcgagta ccgcccggat cgggacgggt
     4321 gagcgggcgc ggccccgccc ccgcgcactc cccggttttg ccttgtttgg accggccgcg
     4381 cgtgcgtata ttgcccccgc gcgccggtcg ggtccggcgc cggcgtccgg gtaaacctga
     4441 aatcaggagc caacggtccc tatgcccgag agcaaaccgt cgaaatttga cctggcggtg
     4501 atcatgcccg tccgcaacga ggagaagtac gtcgggcaga ccctcgagca gttgtacctt
     4561 caggattttc ccatggaccg cctcgaggtg gtggtggccg acggcgggtc gaccgaccgc
     4621 acccgcgaaa tcgtcgagtc ctacaagact cggttcggcg ccctgcggct gctggacaac
     4681 ccggtgcgcc gcccttcctc gggccgcaac atcggcatcc gcaacagcac cgcgccgcgg
     4741 atcgtcgtca tcgacggtca cgtgtacatt ccgagcaaga ccctcctgcg cgacgtcgtc
     4801 gaaaccttcg accgcaccgg cgccgactgt ctctgccggc cgcagccgct cactccgccc
     4861 gacctcaacg atttgcagac ggcgatcgcg ctctgccgcg gctcggcgct cggccacaaa
     4921 cccggatcgg aaatctacgc cgactacgag ggggaggtcg acccgacctc ctccggggcg
     4981 atgtggacgc gcgcgacctt cgaccgggtc gggctgttcg acgagaactt cgacgccggc
     5041 gaggacatcg aactcaactt ccgcgtccac cgggcgaaac tgaaagcctg gctcaacccc
     5101 gcactgagag tctactacta cccgcgcgcg actctgccgg ccttgtggcg gcagatggtc
     5161 cgctacggtc tcgggcgttt ccgcatgtcg cgcaagcacg gactctgggc gcccacccac
     5221 tggctggccg ccgccggcgt cgtcctctgc atcgtcctct tcgtcctcgg gtttctctcg
     5281 ctcgcggtga tgaagctctt ccagattgtc gcggcgctct acctcctcac cgtcattttc
     5341 tattccctca gtctcgcgct gcggcatcgc aatctcaact gcctgctgta cggaccgttg
     5401 attttcccaa ccatccactt cggcctcgga gtgggatttc tcaaaggcgt gatcgaatcg
     5461 tactcccctc cgccggcgcg ggaggccggt ccgccgcagt agctcagccg gcgtcacacg
     5521 agggaggctt gcgtgtccat gctcacgacc ttcagccaca gcgcgctcca gacttttgcg
     5581 cagtgccggc gcaaatttca gttccggtac ctcgagcggc cggaaatccc gaaacggatc
     5641 tccgccgaag cgtacctggg aaccgccgtc cacaccgtcc tccaggcgct ctacgagcgc
     5701 ggcgccgacg gcgtggagct gtcgctcgag gccgccctcg agatctacga gcgggcctgg
     5761 cgcgaggtcg accgcgctct cctggcggtt gtgaccgagc acatgggcat cgacgattac
     5821 atccgcatcg gccgtgaaat cctcgcccgc caccacgagc gctaccggcc gttccggccc
     5881 ggcgccctgc tgggcgcgga gctccacctc cacttcaccc tccccggcac gccctaccgg
     5941 ctgagcgccc gcatcgaccg gttgtggcgg cgggacgacg gcacggtgga gatctgcgac
     6001 tacaagaccg gcaagaacct ttcccgcccg cacgacccgg attatttcca ccagatgggc
     6061 ctctaccagt tggcggtgat ggcgaactac ccgcagtacc gcgacatcga ggcggcccag
     6121 tactgcctgc gcatggacga ggtcgtcgcc caccggttcc gccccgatga tctcgaactg
     6181 ctcgtcgaaa acatccgcca gcagatcctg gcgatccacg aggccgaccg tctcagccag
     6241 tttccgacgc gcgaatcgtc gctgtgcaac tggtgcgagt atgccgcgct ctgtccggcc
     6301 aagcggcaca agctgctgat cgaggccggc gaggaggacc gggacggagc tctgcggcgg
     6361 gcctacgcca aagcgaccga gtatatcgcg cgctacgcgg cggtcaaaga gctcgaggcc
     6421 gaaatggccg ccctgcgggc cgacctcgtg cggctggccg gggagctcca gatgaccaag
     6481 ttctcaggtg aagggggaga ggtcgcggt
//
LOCUS       DAOOKS010000013         6250 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_14896_length_6250_cov_3.040517, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000013 DAOOKS010000000
VERSION     DAOOKS010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6250
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14896_length_6250_cov_3.040517"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3498
                     /locus_tag="PK112_00440"
     CDS             <1..3498
                     /locus_tag="PK112_00440"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPC10550.1"
                     /translation="EDLERVYFLATERPAAAAADITDMLGRGNVEWVEPEYFLELFEL
                     PADSLFPRQWHLLNTGQEYFGINRIDGAYNDERVIKRGTAGRDVRLVGQYQSPPADSA
                     EKVVVAIIDTGVDTDHPELAGRVWRNLDEIPGNGGDDDHNGYIDDTAGYDISGDTSNL
                     FDPAGDADPRDEAGHGTHCAGIVGANGDGAGVVGIAPWIEIMPVKIFPNGTTRIGGLG
                     ILYAVNNGARVINASWGSPFQSQFLREVINFARENGVLVCMASGNSGDNSRFYPAGFD
                     LDSTLVVGAGNSDGELTYFSTFGSHVDIIAPGLDILSLRAAGTDMYAAYPAYEPEVRI
                     VDSLYYLSDGTSMAAPMVCGAAGMLLSFRPDLSLGELVAILKHGATDLVDPLNQGDDL
                     PGPDSLCGWGYLNIDASLGLMESGGMYLTAPEHRSRHTGDIIIKGGTIGGYDDGWTIE
                     YATGLSPEAWHFLAAGNGLPPDSVLAVFRNPGAAGLLSFRLTDSYGRSGTVTVTYAPT
                     NVLAMSSPADGMTFDYAIPVRGSAYGPDFDSLVVKSRGPRGNVVRLFAGTGEVFDSLF
                     YTWPASGADTGAFTLYLYGYFATGVEGDSAGITVTSAFADGWPRRIPGWGGMTAVSAD
                     LNRDGSKELIAATSGGLAVYRAADGELLSGFPALAGRDVRCVPAVYDVDGDGHDEIIV
                     TDSTGLHVIRDDGTAAPGWPQACFTGMTPYEYGFPAPVIVELRRGSPPGAVPDSGILF
                     INRLGQLIAFRFNGDRYFYSRKMWGQFDARISPSYLTGGDTSPFATAADVDGDGRREV
                     CASFSSANPHTGMGLFDGANGRPAFGRDVPTIQTIASVSGSVLADLDGDGLPEAITTG
                     LGDDDLEHVWIKTLGTDDFASWVVPIDDSWIGWIASYPVVADLDRDGTPEILMTYFEF
                     DQAALFIFRADGTPYATGPRGIPGEAFYDYATFATPAVANIMGDEYPEIIFRAGHLLP
                     GTGPELLYILDYAAVPVAGWPKRTPAPSATVASGRQVPLVDDLDNDGKVELALLSDEQ
                     SLLVWDFEGSYDEGRNTFRWMYDNGNSGIYPGSQAAPQSSDGPARVRKAQRSAAGEAR
                     AVGRATAPVRFNLPAPARVAVEIYDTAGKRVATLPERMMEAGDQEMDFSEAALAPGTY
                     FYRLIVDGTAFSRRMAVIE"
     gene            3495..5645
                     /locus_tag="PK112_00445"
     CDS             3495..5645
                     /locus_tag="PK112_00445"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HPC10551.1"
                     /translation="MTRQSAAAVLAVVLVLAAPTGSPWADLADGPVHTILWNRMDLVE
                     VVDSLAVGFSSEGVTVCRWSDSLNQFVVESVHEIDYYPAAVRMAEGRLMVHTADQRLI
                     AYDAAMLPELVTAWEFAPGAAFADWALVDTQLYLSRWFEGLWRYAIGPAGTAALLDTS
                     MVGIMVTQMEAEGPWLYVLDEYNGIMRYDPAGAGSAAFLDYLYVPQEVLSFAQADSTS
                     AMIGRSGGVVIGRYPGPQSLVAPAVPDGDRPQRVYLSGDAMVTVAPRSIEVRDRGTGE
                     VRAAMAIEGVVTDGALFTRGGTRYLVLPREAGGLFLVNIDDLGQSGPGLYRSGPVQAL
                     AFGGGKLFTSGGHNPIEAYTFDSTLAPHLDFALLPHLNSMQALTVTGDTLLAYMAGIN
                     TIVVIRRPFFADSFLVDRAITLDDTMAGRIVFLPRLRHDSLRAIAVTGLRTMQVFTIN
                     DSGRVSSQEPWEQQFGITASCLTDTMVFLATGKDQIWSYRIGPDLRTQLAGITDLPSQ
                     ASVMVWDSGRLLVFVRDQLLVSRLLPSGALSVESWIDMPFTASDAVISGRGLFAVGTT
                     GIGVFRLDREVPELAEYGGDPGTVIAVSGEMVAASDGGSVKIYRLGVATGVDPEPELP
                     RRFALDQNYPNPFNGETVIRYSLAEGARVRLAVYNVLGQRVKTLVDEYRAAGIYEALW
                     DGTDRAGAPVASGVYLYRLEAGKAAGARKMVFVK"
     gene            complement(5651..>6250)
                     /locus_tag="PK112_00450"
     CDS             complement(5651..>6250)
                     /locus_tag="PK112_00450"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HPC10552.1"
                     /translation="LFDITSGVLRRAWKFDGSTASPDRDLIESLLGRVGWEKVEWDGR
                     RLRLQPGMEIDLGGLGKEYAADRAADLAFRASGAPLMANFGGDIRALSADPDSPPWVV
                     GIEDPLREGTAVGRIDLSNGGVATSGDARRFCLVEGVRLGHILNPRTGWPVADAPRSV
                     TVLGDYCVEAGFLATLAMLHGRAAEDFLAGEKVPYHCIR"
BASE COUNT          989 a         1965 c         2226 g         1070 t
ORIGIN      
        1 gaggatttgg agcgcgtata cttcctggcg actgagcggc ctgccgcggc ggccgccgac
       61 atcacggaca tgctcggccg cggcaatgtc gaatgggtgg agccggaata cttcctcgaa
      121 ctcttcgaac tgcccgccga ttccctgttc ccgcgccaat ggcatctgct gaatacaggg
      181 caagagtatt tcggaatcaa ccgcatcgat ggggcataca acgatgagcg cgtgatcaag
      241 cggggtacgg cggggaggga cgtgcgcctg gtcggccagt accagtcgcc gccggcggat
      301 tcggccgaga aagtggtcgt cgcgatcatc gacacaggcg tggatacgga tcacccggaa
      361 ctggccggac gggtctggcg gaacctcgat gaaattcccg gcaacggcgg cgacgacgat
      421 cacaatggat atatcgacga caccgccggg tacgatattt cgggggatac cagcaacctc
      481 ttcgatccgg cgggtgacgc cgacccgcgc gacgaggccg gtcatgggac gcactgcgcg
      541 gggatcgtgg gagcgaacgg cgacggcgcg ggagtggtgg gaatcgcgcc gtggatcgag
      601 atcatgccgg tgaagatttt cccgaacggg accacgagga tcggcgggct cggcatcctc
      661 tatgccgtga acaacggggc gcgggtgatc aacgcgagct ggggctcgcc cttccaatca
      721 caattcctgc gcgaggtcat caacttcgcg cgggaaaacg gcgtgctcgt ctgcatggcc
      781 tcgggcaaca gcggcgacaa cagccggttc tacccggccg ggttcgacct ggattcgacg
      841 ctggtcgtgg gggccgggaa ctccgacgga gagctgactt acttctcgac cttcggctcg
      901 cacgtcgaca tcatcgcccc gggcctggac atcctgtcgc tgcgggctgc ggggaccgac
      961 atgtacgccg cctacccggc ctacgagccg gaggtgcgga ttgtcgacag cctctactac
     1021 ctctcggacg ggacgtcgat ggcggcgccc atggtgtgcg gggcggccgg aatgctgctg
     1081 tcgttccgcc ccgacctgtc gctcggcgag ctcgtggcca tcctgaagca cggggcgacc
     1141 gacctggtcg atccgctcaa ccagggggat gatctgcccg gcccagactc gctctgcggc
     1201 tgggggtacc tcaatatcga cgcctccctc ggtctgatgg aaagcggcgg catgtacctc
     1261 accgcgcccg aacaccgcag ccgccacacc ggcgacatca ttatcaaggg aggaacgatc
     1321 ggcggctacg acgatgggtg gacgatcgag tacgcgaccg ggttgagccc ggaggcctgg
     1381 cacttcctcg ccgccggcaa cggcctgccg cccgattcgg tgctcgcggt ttttcgcaac
     1441 cccggcgcgg cgggactgct cagtttccgc ctcacggaca gctacgggcg cagcgggaca
     1501 gtgacggtga cgtatgcgcc gacgaatgtg ctggcgatgt cgtcgccggc cgacggaatg
     1561 acgttcgact atgcaatccc cgtcaggggg tcggcttacg gacccgattt cgactcgctg
     1621 gtggtcaaga gccgcggacc gcgcgggaat gtcgtgcggc tttttgcagg gaccggggag
     1681 gtatttgact cgctcttcta tacctggccg gcctcggggg ccgatacagg ggcgttcacc
     1741 ctgtacctct acggttattt cgcaaccggt gtggaagggg attcggccgg gatcacggtg
     1801 acttcggctt tcgccgacgg atggccgcgg cggatccccg gctggggggg gatgacggcg
     1861 gtgagcgcgg atctcaatcg ggacggttcc aaagaactga tcgcggccac gtccggggga
     1921 ctcgcggtgt atcgggcggc cgacggagaa ctgctgagcg gcttcccggc gctcgcgggg
     1981 cgcgacgtgc gctgcgtgcc ggccgtgtat gatgtggacg gcgacggcca tgacgagatc
     2041 atagtcaccg attcgaccgg gctccacgtg attcgcgacg acggcacggc cgcgcccggc
     2101 tggccgcagg cctgtttcac gggcatgacc ccctacgaat acgggttccc ggcgccggtg
     2161 attgtcgagc tgcgccgcgg ttcgcccccg ggagcagtgc ccgattcggg catcctgttc
     2221 atcaaccggc tgggacagct cattgccttc cgcttcaatg gcgacaggta cttctactcg
     2281 cggaagatgt ggggtcaatt cgatgcgcgg atatccccct cgtacctgac cgggggagac
     2341 acctcgccct tcgcaacggc cgcggatgtc gacggcgacg ggcggcggga agtgtgcgcg
     2401 tccttcagct cggccaaccc ccacaccggc atggggctgt ttgacggggc caacggccgc
     2461 ccggctttcg ggagggacgt cccgaccatt caaacaatcg cctcggtgag cggcagcgtc
     2521 ctggcggatc ttgacggcga cgggctgccg gaggcgatca cgaccggcct gggcgacgat
     2581 gatctcgagc acgtgtggat caagactctc ggaaccgatg atttcgcgag ctgggtggtg
     2641 ccgatcgacg actcgtggat cggctggatc gcctcctacc cggtggtcgc cgatctggat
     2701 cgcgacggga cgccggaaat tctcatgacc tatttcgaat tcgatcaggc cgcgctcttt
     2761 attttccggg cggacggcac gccgtacgcg acgggaccgc gcggaattcc cggggaggcg
     2821 ttttacgact acgcaacctt cgcgaccccg gccgtggcga atatcatggg ggacgagtac
     2881 ccggagatca tcttccgggc cgggcacctg ctgccgggga ccggtccgga gctgctctac
     2941 atcctggact atgcggccgt gccggtggcc ggctggccga aacggacgcc cgcaccgtcg
     3001 gcgaccgtgg cctcggggcg gcaggtcccg ctggtcgatg acctcgacaa cgacggcaaa
     3061 gtcgaactcg ccttgctgag cgacgagcag agcctgctgg tgtgggattt cgaggggagt
     3121 tacgacgagg ggcggaacac gtttcgctgg atgtacgaca acgggaacag cggcatctac
     3181 ccggggtcgc aggctgcgcc gcagagttcg gacggcccgg ctcgggtgcg caaggcgcag
     3241 cggtcggcgg ccggcgaggc gagggcggtc ggtcgggcga cggcgccggt gcgcttcaat
     3301 ctccccgccc cggcgcgtgt ggcggtcgag atttacgaca ccgccggcaa gcgggtcgca
     3361 acgctgccgg agcgaatgat ggaagcgggg gaccaggaga tggatttctc cgaggcggcc
     3421 ctcgcgccgg gaacgtattt ctaccggctg atcgtggacg gcacggcgtt ttcgcggcga
     3481 atggcggtga tcgaatgaca cggcaatcgg cggcggctgt tctcgcggtc gttctggtgc
     3541 tggcggcgcc gacagggtcg ccttgggcgg acctggccga cgggccggtg cacaccattc
     3601 tgtggaaccg gatggacctc gtagaggtcg tcgattcgct cgccgtgggg ttctcgtccg
     3661 aaggggtgac ggtctgccgg tggtcggaca gcctcaacca gtttgtcgta gagagcgtgc
     3721 acgagattga ctactatcca gcggccgtgc ggatggcgga gggacgcctc atggtgcaca
     3781 ccgccgacca gcgactgatt gcgtacgacg cggcgatgct gcccgaactg gtgaccgcct
     3841 gggagttcgc tcccggggcg gcgttcgccg actgggcgct cgtcgacacc cagctctacc
     3901 tgtcgcggtg gttcgagggc ctgtggcgct atgcgatcgg accggccgga acggccgcgc
     3961 tccttgacac gagcatggtg gggattatgg tgacgcagat ggaggcggag ggaccctggc
     4021 tgtatgtcct cgacgaatac aacgggatca tgcgctacga cccggccggg gcggggtcgg
     4081 cggcctttct cgattacctc tacgtgcccc aggaagttct ttcgttcgcg caggcggact
     4141 cgacttcggc catgatcgga cggtccggcg gggtcgtcat cgggcggtat cccgggccgc
     4201 agtcgctcgt cgcgcccgcg gttcccgacg gcgaccggcc gcagcgggtg tacctgagcg
     4261 gggatgcgat ggtgaccgtg gcgccgcgct ccattgaggt gcgcgaccgg gggaccggcg
     4321 aggtgcgggc cgcgatggcg attgagggcg tggtgaccga cggcgcgctg ttcacccggg
     4381 gtggaacccg ctacctggtg ctgccccgcg aagccggcgg gctgtttctg gtgaatatcg
     4441 atgacctcgg gcaaagcggg ccggggctgt atcgctccgg gccggtgcag gcgctggcgt
     4501 tcggcggcgg caaactgttc acgtcgggcg gccacaaccc gatcgaggcc tacacgtttg
     4561 actccacgct cgcgccccac ctggacttcg ccctcctgcc ccacttgaac agcatgcagg
     4621 cgctgacggt gacgggcgac actctcctgg cctacatggc cggcatcaac accattgtgg
     4681 tgatccgccg gcccttcttc gccgattcct tcctcgtcga ccgggcgatc accctggacg
     4741 acacgatggc ggggcggatc gtctttctgc cccgcctgcg gcacgactcc cttcgggcga
     4801 tcgccgtcac ggggctgcgc actatgcagg tctttacgat caacgactcc ggccgggtca
     4861 gttcgcagga gccgtgggag cagcagttcg ggatcaccgc atcctgcctc acggacacga
     4921 tggtcttcct ggccacgggg aaagaccaga tctggtcgta tcgaatcggg ccggatctcc
     4981 gcacgcagtt ggccggcatc accgaccttc cctcgcaggc ctccgtgatg gtctgggaca
     5041 gcggccgcct cctcgtgttc gtccgcgacc agcttctcgt gtcccgtctc ctgccgagcg
     5101 gagcgctctc tgtcgagagc tggatcgata tgccgttcac ggcgagcgat gcggtcatct
     5161 cggggcgggg gctgtttgcg gtcggaacca cggggatcgg ggtcttccgt ctcgaccggg
     5221 aagtgccgga attggcggag tacggcgggg acccgggcac cgtcatcgca gtgtcgggag
     5281 agatggtcgc ggcctcggac ggcggttctg tgaagattta ccgcctgggc gtggccacgg
     5341 gcgtggatcc cgaaccggaa ctgccgcggc ggttcgcgct cgaccagaac tacccgaatc
     5401 cgttcaacgg tgagacggtg atccgctatt ctctggcgga gggagcgcgc gtgcggctcg
     5461 cggtctacaa cgtgctcggg cagcgcgtga aaacgctcgt ggatgagtac cgggcggcgg
     5521 ggatctatga agcgctgtgg gacggcacgg atcgcgcggg ggcgccggtg gcgagcgggg
     5581 tctacctcta ccggctcgag gccgggaagg ccgccggggc caggaagatg gttttcgtga
     5641 agtagggggc ctaccggatg cagtggtaag ggactttttc tccggcgagg aagtcctcgg
     5701 ccgcccggcc gtgcagcatg gccagggtgg ccaggaaccc ggcctcgacg cagtagtctc
     5761 ccagaaccgt gacacttcgg ggagcatcgg ccaccggcca cccggtgcgg gggttgagaa
     5821 tgtgacccag ccgcacgcct tccaccaggc agaaccgccg cgcgtcgccg ctggtggcga
     5881 cgccgccgtt gctgaggtct attcgaccga cggcggttcc ctcgcgcagc gggtcctcga
     5941 tgcccacgac ccacggcggc gagtcggggt cggcgctgag ggcccggatg tcgccgccga
     6001 agttggccat gaggggggcc cccgaggcgc ggaacgcgag gtcggccgcc cgatcggccg
     6061 cgtactcttt gccgagaccg ccgagatcga tttccatgcc gggttgcagg cgcaggcgcc
     6121 gcccatccca ttcgaccttc tcccacccga cccgtccgag cagcgactcg atcaggtctc
     6181 ggtccggcga agccgtcgat ccgtcgaatt tccaggcccg gcgaaggacg ccggaggtga
     6241 tgtcgaagag
//
LOCUS       DAOOKS010000014         6240 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_14929_length_6240_cov_3.454810, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000014 DAOOKS010000000
VERSION     DAOOKS010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6240
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14929_length_6240_cov_3.454810"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="PK112_00455"
     CDS             complement(<1..823)
                     /locus_tag="PK112_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HPC10553.1"
                     /translation="MSRSDIALAIGVIGIIGVLVIPIPTPLLDFALAFNITFSLVVLL
                     TTLYVTRPLDMSVFPGMLLIVTLMRLALNVASTRLILGQAFAGDVIQTFGNFVVQGNY
                     VVGFIVFVILVIIQFVVITKGAGRISEVAARFTLDAMPGKQMAIDADLNAGIITEEEA
                     RARRRDIAREADFYGAMDGASKFVRGDAIAGILITLINIIGGFIIGIAINGMDINEAL
                     RTYSLLSIGDGLVTQIPALLVSTASGIIVTRAAADSNMGTDLTRQLTRQPRAVMVA"
     gene            complement(867..1937)
                     /gene="flhB"
                     /locus_tag="PK112_00460"
     CDS             complement(867..1937)
                     /gene="flhB"
                     /locus_tag="PK112_00460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HPC10554.1"
                     /translation="MADESFQERTEKATSRRREKAREEGQVAKSMELNSAAVICLGFV
                     TLYLMGPYIAGQALALMRATMANAPSLALADPTFTGVFRNHLLKFFIILGPVFAVLTV
                     IAVGVNVAQVGFRITPKAAAPQLDKLDPVKGFKRIFSVRSAVTLLRDLLKLTVVAFVA
                     YRVIAAEFPEFYRFADMTVAQIAAAMGKLALELALKVGAVILVIAVLDYIYQRYEFEK
                     SIRMSKQDLKDEFKDTEGSPQIKARVRQIQLQIARSRMMEAVPTADVVITNPTHYAVA
                     LKYAPEEHRAPHVVAKGQRLVAQKIKEIALAHNVPCVEDRPLAQALFKMCDVGQMIPA
                     ALFRAVAEVLAYVYRLKGQGVH"
     gene            complement(1943..2731)
                     /gene="fliR"
                     /locus_tag="PK112_00465"
     CDS             complement(1943..2731)
                     /gene="fliR"
                     /locus_tag="PK112_00465"
                     /inference="COORDINATES: protein motif:HMM:NF013478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HPC10555.1"
                     /translation="MFEFVNFGANALQVFLLVIMRASGLFLMAPVFGDKSVPVLVRIG
                     LIILLAGVIASVIRQPQLPAFDSLWVLAGFAVKEVLVGLIIGLVFRLLFMGVETGGAL
                     VGYQMGFAMVELPDTDGSSQMPVIGKFWVLTATVIFFVIGGHHLVLRAFADSYEVIPP
                     GVAGTSGAVGELLIKYTAYVFVIAIKIAAPVMITLFLADVALGVIAKTMPTMNVFFVG
                     IPIKIAAGLAMLALSLPLFGYVLEKVTLHLDRELRVLYLAFGKA"
     gene            complement(2986..4017)
                     /locus_tag="PK112_00470"
     CDS             complement(2986..4017)
                     /locus_tag="PK112_00470"
                     /inference="COORDINATES: protein motif:HMM:NF024937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HPC10556.1"
                     /translation="MQTLSLLGSMFGLALLSGLNLYAAALTVGLAVRYNIISLPLPLQ
                     GLEVLAHPAVLVIAGLLYAVEFIADKIPWVDSAWDAVHTFVRPLGAAALGLAAFQGMD
                     PVWETAAALVCGGLSLSSHSTKAATRLIVNQSPEPVSNSLVSLFEDVLVVVGSLLFLR
                     YPALGMAAVGLFLALMFWLGPKLLRLAKMEFAAVTGSLRGDEGAPVLSDTDLPDPFRR
                     ALNALPPTDPPRRRAWVYSGKGFSGGRFYAGLLVSGPARLAFITRRWFKVRTYEIALS
                     ATARIEFRKRPVLSVMHIESGRRVIKFYGTRDKAAALEALAEGLRAQLGRGEAAASSP
                     SSDPRTDLS"
     gene            4320..5735
                     /locus_tag="PK112_00475"
     CDS             4320..5735
                     /locus_tag="PK112_00475"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HPC10557.1"
                     /translation="MSISHRALVAGGRKARPSRLRAVVCAALTLVLLAAPGRSAAGAP
                     GQLGTGRALIPITPENAPNVALLATLSEHTGGVWTAAFSPDGRQLASCGEDGRVIIRV
                     VDSLSHTRTLGDSEYWVVGLAFSPDGSQLAAGEADGFGGAYGPIRIWDAAADTLVRTL
                     SGHYGGVWSLDYQESTGMLASGSFDGTVRIWDPQTGAPVRILSGHTAAVLAVDFSPTQ
                     DLLASSGVDFTVRLWQPATGDTVRVLRGHTGNIGYVEFSPDGLALASSADDRTVRLWD
                     FSDGSLLWSRAAGQGWVNCVTFSGDGRLMATCGHDGSVVLRDASTGAELKRLAGHQGP
                     VLRGAFSPDGTLLATASWDLTVRLWGVETDLDGDGADNDSDNCPNVPNPGQEDADGDG
                     IGDACCCTVRGDCTGDAAVGVADLTYFIRYLFRGGPAAGCPSHADINADETVGLADLT
                     CLIGFLFREGPAPAACPSAWR"
     gene            complement(5781..6050)
                     /gene="fliQ"
                     /locus_tag="PK112_00480"
     CDS             complement(5781..6050)
                     /gene="fliQ"
                     /locus_tag="PK112_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004360999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HPC10558.1"
                     /translation="MTQEMVVAIGREALSVTLLISGPMLLFGLIVGLVISIFQAVTQI
                     HEMTLTFVPKILAVALALLIFLPWMINIATDFTRHMFDLIPQLVG"
     gene            complement(6120..>6240)
                     /locus_tag="PK112_00485"
     CDS             complement(6120..>6240)
                     /locus_tag="PK112_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017437074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliP"
                     /protein_id="HPC10559.1"
                     /translation="MGMMMLPPVIVSLPFKILLFVLVDGWYLLVKSLVESFRM"
BASE COUNT         1165 a         1890 c         2215 g          970 t
ORIGIN      
        1 cggcgaccat gaccgcgcgc ggctggcggg tgagctggcg ggtgaggtcg gtgcccatgt
       61 tcgagtcggc ggcggcgcgg gtgacgatga taccggaggc ggtcgaaacc agaagggccg
      121 ggatctgcgt caccaaaccg tcgccgatcg acaagagcga gtaggtgcgc agggcctcgt
      181 tgatatccat gccgttgatg gcgatgccga tgatgaagcc gccgatgatg ttgatcaggg
      241 tgatgaggat tccggcgatg gcgtcgccgc ggacgaattt cgaagcgccg tccatggccc
      301 cgtagaaatc agcctcgcgg gcgatatccc ggcggcgcgc ccgcgcctct tcttctgtga
      361 tgatccccgc gttgaggtcg gcgtcgatgg ccatctgctt gccgggcatg gcgtcgaggg
      421 tgaaccgggc ggcgacctcg gagatacgcc ccgcaccctt cgtgatcacc acgaactgga
      481 tgatgacgag gatgacgaag acgatgaagc cgacgacgta gtttccctgg acgacgaagt
      541 tgccgaaagt ctggatcacg tcgccggcga aggcctggcc gaggatgagc cgggtcgagg
      601 cgacgttgag ggcgagccgc atgagggtca cgatgagcag catgccggga aagaccgaca
      661 tgtcgagcgg ccgcgtgaca tagagggtgg tgagcagcac gacgagcgag aaggtgatgt
      721 tgaaggcgag ggcgaaatcg agcagcggcg tggggatcgg gatgacgagc acgccgatga
      781 tgccgatcac gccgatcgcc agggcgatgt cggagcggct cacgagcagg tcgagcaggc
      841 cggtatggtc tttccccgcc ttcatctcag tgcactccct gtcccttgag gcggtagacg
      901 taggcgagca cctcggccac ggcgcggaag agcgcggccg ggatcatctg gccgacgtcg
      961 cacattttga agagggcctg ggcgaggggg cgatcctcga cgcaggggac gttatgggcg
     1021 agggcgattt ccttgatctt ctgcgccacc agccgctgcc ccttggcaac gacgtgcggg
     1081 gcgcggtgct cctcgggcgc atacttgagg gcgaccgcgt agtgggtcgg gttggtgata
     1141 acgacgtcgg cggtcggcac cgcctccatc atgcggctgc gggcgatctg gagctggatc
     1201 tgccgcaccc gggccttgat ctgcggcgac ccctcggtgt ccttgaactc gtccttgagg
     1261 tcctgcttgc tcatgcggat agacttctcg aattcgtagc gctggtagat gtagtcgagg
     1321 acggcgatga cgaggatgac cgcaccgacc ttgagcgcca gttcgagcgc aagcttgccc
     1381 atggcggcgg cgatctgcgc caccgtcata tcggcgaagc ggtagaattc ggggaattcg
     1441 gcggcgatga cgcggtaggc gacgaacgcg accacggtga gtttcagcag gtcgcgcagg
     1501 agcgtgaccg ccgagcggac cgagaagatg cgcttgaaac ccttgaccgg gtcgagcttg
     1561 tccaactgcg gcgcagccgc cttgggggtg atgcggaaac cgacctgggc gacattcacg
     1621 ccgaccgcga tcacggtcag cacggcaaac accgggccga ggatgatgaa gaacttgagg
     1681 aggtggttgc ggaagacgcc ggtgaaggtc gggtcggcca gggcgagact gggcgcgttg
     1741 gccatggtcg cccgcatgag cgccagcgcc tggccggcga tgtacggccc catcaggtag
     1801 agggtgacga agccgaggca gatcacggcg gccgagttca actccatcga cttcgcgacc
     1861 tgcccctcct cgcgcgcctt ctcccggcgg cgtgaggtcg ccttctcggt tctttcctgg
     1921 aaactctcgt ccgccatttc ccttatgcct taccgaatgc gaggtagagc acgcgcaatt
     1981 cccgatcgag gtggagcgtc actttctcca gcacgtagcc gaagagcggg agcgacaggg
     2041 caagcatggc cagcccggcc gcgattttga tcgggatgcc gacgaaaaag acattcatcg
     2101 tcggcatcgt cttggcgatc acgccgaggg cgacgtcggc gaggaagagc gtgatcatga
     2161 ccggggcggc gattttgatc gcgatgacga acacgtaggc cgtgtacttg atcagcagtt
     2221 cgccgaccgc ccccgacgtc ccggcgacac cgggcgggat gacctcgtag ctgtcggcga
     2281 aggctctcag cacgaggtgg tggccgccga tcacgaagaa gatcacggtc gcggtgagga
     2341 cccagaactt gccgatcacc ggcatctggc tggagccatc ggtgtccgga agctcgacca
     2401 tcgcaaatcc catctggtag ccgacgagcg cgccgccggt ttcaacgccc atgaacagga
     2461 ggcggaaaac cagcccgatg atcagcccga ccagaacctc cttgaccgcg aacccggcca
     2521 gcacccagag cgagtcgaaa gccgggagct ggggctggcg gatgacggag gcgatgacgc
     2581 cggccaggag gatgatcagg ccgatccgca ccaggaccgg gaccgacttg tcgccgaaca
     2641 ccggcgccat caggaagagg ccggaggccc gcatgatgac gaggaggaag acctggagag
     2701 cgttggcgcc gaagtttacg aattcaaaca agggtcacaa tcctggttta tcggttatcg
     2761 ccttttgcta tgtcaaatgg tgtgccgaag tgaaacggcg ggggcggtac aggtaagtcg
     2821 ttgggagaaa gcatcttgcg ggacgggaaa aatcgggcgg gggccgccgg agagcggact
     2881 gcggaaaggt ttaccagcag ttgtgggaaa aaagttcaga gcgcggaaag gccgcggccg
     2941 ggatgtcggc ggccacggac cggggagcag ggcgggcgat aggggttacg acaggtcggt
     3001 gcgcgggtcg gaggatggcg aagacgcggc ggcctctccc cgcccgagct gcgcgcgcaa
     3061 cccttcggcc agcgcctcca gggcggcggc tttgtcccgc gtgccgtaga acttgatgac
     3121 ccggcgtccc gactcgatgt gcattaccga gagcacgggg cgtttacgga attcaatgcg
     3181 cgccgtcgcg gacaacgcga tttcgtaggt acgtactttg aaccaccgcc gcgtgatgaa
     3241 ggccagccgc gcggggccgg agacgagcag cccggcgtag aaccgcccgc ccgaaaatcc
     3301 cttgcccgaa tatacccagg cccgccgccg cggcggatcg gtcggcggaa gggcgttgag
     3361 ggcccggcgg aaggggtcgg ggaggtcggt gtcagaaagg accggggccc cctcatcccc
     3421 gcgcagcgat ccggtcaccg cggcgaactc cattttcgca agacggagga gtttcggccc
     3481 gagccagaac atcagcgcca gaaacagccc gaccgccgcc atccccagag cgggatagcg
     3541 caggaagagg aggctgccga cgacgacgag gacatcctca aacagactca ccaggctgtt
     3601 ggagaccggt tccggactct gattgacgat gagccgggtc gccgctttcg tcgagtggga
     3661 cgacagactg aggccgccgc aaacaagcgc ggcggcggtc tcccacaccg ggtccatccc
     3721 ctggaaggcg gccagaccga gcgcggccgc gccaagcggt cgaacaaacg tatgcacggc
     3781 atcccacgcc gaatccaccc aggggatctt gtcggcgatg aattcgaccg cgtacaggag
     3841 accggcgatc acgagcacgg ccgggtgggc aagcacctcc agaccctgca gggggagggg
     3901 gagcgaaatg atgttgtagc ggacggcaag tccgaccgtc agcgcggcgg cgtacaggtt
     3961 gagacccgac agcagcgcca gaccgaacat cgaccccaaa agcgagagtg tctgcatgcg
     4021 gcaggtttcc ttctgcacgc aagctaccgc tctgccgagg aactgtcaac agcgttcggc
     4081 caacgggaca acggcccaca ggcaagtcac catggagatg ccccagagcg gacatttcgc
     4141 aggcggttac gaaacatgcc cgcggaggtc tccgtatggg agactgggat tgatcctgcc
     4201 gatggggatt cggcgggcgg ccgccctcgt acagacgcgc gaggcgtgcg gagcggagtc
     4261 acatcatccg cacgcaaacg aggcggcgcc gcgtgcgccg caccaggtcg gaggtcggca
     4321 tgtccatctc gcacagagct ctggtcgccg gtggtcggaa ggcacgacca tcccggctgc
     4381 gggcggttgt ctgcgccgcc ctcactctcg tcctgctcgc cgcgccgggc agatccgcag
     4441 ccggggctcc cggtcagctc gggaccgggc gcgccctgat tcccatcacg cccgaaaacg
     4501 ccccgaatgt cgcgttgctg gcaacgctgt cggaacacac gggcggcgtg tggacggcgg
     4561 cgttcagtcc cgacggccgt caactggcct cctgcggcga ggacggccgg gtgatcatcc
     4621 gcgtggttga ttcactgtcg cacacccgga cactgggcga ttccgagtac tgggtggtcg
     4681 ggctggcgtt cagtcccgac ggttcgcagt tggccgccgg ggaggccgac gggttcggag
     4741 gagcttacgg cccgatccgc atttgggacg cggcggccga taccctcgtg cggacgctgt
     4801 cggggcatta cgggggcgtc tggagtctcg actaccagga gagcacgggg atgctggcct
     4861 cgggcagctt cgatgggacg gtgcggatct gggacccgca gaccggcgcg ccggtgcgca
     4921 tcctgagcgg acacacggcg gcggtgctcg cggtagattt cagccccacc caggacctgc
     4981 tcgcttcgtc gggagtggat ttcacggtac gcctgtggca acccgcgacc ggcgacaccg
     5041 tgcgcgtgct ccgcgggcac acgggcaata tcgggtacgt ggagttcagt ccggacggat
     5101 tggcgctggc ctcatcggcc gacgaccgga cggtccggtt gtgggatttt tcggacggca
     5161 gtctgctctg gtcgcgcgcg gccggccagg gctgggtgaa ctgcgtgact ttcagcggcg
     5221 acgggcgcct aatggccacc tgcggccacg acggctcggt ggtgctgcgc gacgcctcca
     5281 ccggggccga gctcaaacgg ctggcgggac accagggtcc ggtgctgcgc ggggcgttca
     5341 gccctgacgg cacgctgctg gccaccgcct cgtgggacct cacggttcgg ctctggggcg
     5401 tggagacgga cttagacggc gacggagcgg acaatgacag cgacaactgc ccgaacgtcc
     5461 ccaatccggg gcaggaggat gccgacggcg acgggatcgg cgatgcctgc tgctgcactg
     5521 tgcggggcga ctgcaccggc gatgcggcgg tgggagtcgc ggatctcacg tacttcatca
     5581 gatacctctt ccgcgggggg ccggcggcgg gttgcccgtc gcacgcggac atcaacgcgg
     5641 acgaaacggt gggactggcg gacctgacct gcctgatcgg ctttttgttc cgcgaagggc
     5701 cggctcccgc ggcctgccct tcggcctggc ggtgagggga gggtcgtgtg ccgccggagg
     5761 gcgccgacgg ccggacagct ctaccccacg agctgcggga tgaggtcgaa catgtgtcgc
     5821 gtgaagtcgg tcgcgatgtt gatcatccag gggagaaaga tcagcagcgc gagcgcgacc
     5881 gcgaggatct tgggcacgaa ggtcagcgtc atctcgtgaa tctgcgtgac cgcctggaag
     5941 atcgagatca cgagcccgac gatcaggccg aacaggagca tcgggccgga gatcaagagg
     6001 gtcaccgaaa gggcctcgcg gccgatggcc accaccattt cctgggtcat atgatcagtt
     6061 tctccgtcaa tggaccggtc gtcatcacgc cgggttcatg agggtcccgg ccaccttcgt
     6121 tacatccgga acgactccac gagcgatttc accagcaagt accagccgtc cacgagcaca
     6181 aacaggagga tcttgaacgg cagcgatacg atcaccggcg gcagcatcat catccccatc
//
LOCUS       DAOOKS010000015         6119 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_15343_length_6119_cov_3.310521, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000015 DAOOKS010000000
VERSION     DAOOKS010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6119
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15343_length_6119_cov_3.310521"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="PK112_00490"
     CDS             <1..472
                     /locus_tag="PK112_00490"
                     /inference="COORDINATES: protein motif:HMM:NF020660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1926 domain-containing protein"
                     /protein_id="HPC10560.1"
                     /translation="RAGMTAIGNWETQEDIAVDYTLRCGAGRSAEVVFGIENNWTFQA
                     GHAEDRYVLIDGVRLPDSYLDGAAVHREVRTVLLADGCRRLQVGIVSDRPYEIWRLPI
                     WTVSQSEQGFERVYQGTTVVAVYRLRLTEQPTVLSLRLAAGGLAEASAHRPRLD"
     gene            527..1267
                     /locus_tag="PK112_00495"
     CDS             527..1267
                     /locus_tag="PK112_00495"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HPC10561.1"
                     /translation="MVIDEIVRIMRGSRRCVVLTGAGISAESGVPTFRGKEGLWGKFR
                     PEELATMEAFLANPAIVWEWYNWRRTVLGSVQPNPGHFALADMQELFDSFVLVTQNVD
                     GLHRQAGSREVLELHGNIRRNKCLDCAEPHADSADIDPEAIPACPRCGGKIRPDVVWF
                     GEMLPEAVIERAFAESARADVFFSVGTSAIVQPAALLPVEAKHGGAALIEVNPERTPL
                     SHLADLHVSAASGEFLPRLVEAFRTARA"
     gene            1338..3479
                     /locus_tag="PK112_00500"
     CDS             1338..3479
                     /locus_tag="PK112_00500"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPC10562.1"
                     /translation="MIHTMDHFRTMIARVVVPVLLLLAAGCGAYYNTFYNAKRAFNQA
                     ESERRNTKPGQPERILTGDYQTAIEKSLKVVENHPNSKYYDDAVFVLGVSYYYTNQFA
                     KAERRFREILANYPDGQYARDSKLYLAKAMLRQDAVAEAMDLFAELFESDLDRSFKAE
                     AAMALGKYQFEQGRYQLANRYFLAIRDSLGADIDKRVAQRYIADGLMAQFRYNDALAA
                     YLQVLGMDPAPPERYHAMFSAAQAAFRLQRIDVGQDYLSTLAKDNLYFDSLSSLRLAM
                     ADGYEWQGNLIKAEEMYRLVSDEAANRDHKAQANLALGLIYQYDYDDLAKAKEFYDAA
                     AAASRGSSWGQEALQLSSNIGKLETFARTVKLDSTTTQAQIDDAAYTQYQLAELYWYQ
                     LSKPDSAMAEMQYLVDSFPTAYDAPKAMIALAQMAWQSGPDSARGDSIIREMLGRYPH
                     SDFVPEALGLLGLAGTEADTGYAEWYIRRAENFAVDDENIDSALANYQVVTERFPESK
                     FYLQAKFAGIWLTETYRSPGDSSILWAYQAIADSFPSTEWGQAARSKLTGQPIRTLPK
                     AQPADTVTGSRFNITDSSAIDTMAEGESSDTVSMDDKYLAIITAPDGSRCVDIPPNVG
                     IIETRKEFVYPSEAYVSRWEGQLHFQIRLDFSGEVQQAIPKTFADVQEINIRAKETVE
                     STVFDMRLIRPELLDRWFLYVFTVNLPSHLR"
     gene            3476..4270
                     /locus_tag="PK112_00505"
     CDS             3476..4270
                     /locus_tag="PK112_00505"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HPC10563.1"
                     /translation="MMQATCVDTHVLYKHDLSHGYFALTLAGYPRAGECRPGQFLHVR
                     LPGSEVFFRRPMSVAGVNGDRGEIDIIFKVLGRGTRLMSALHDGDPVNLLGPLGKPFR
                     PPDDGSTVVMVAGGVGFPPLLFLAEWLVQGGWDPARIEFLYGGRSTGDILCRDRIRAL
                     GVRFHPTTEDGSLGRRGLVTEPLEDLIRERGAAGLFVYGCGPEPMLKAVNDLGIARRL
                     RGELSLEAPMPCGIGVCLGCVVPRTNGEHARVCLEGPVFDIGEIAL"
     gene            4267..5184
                     /locus_tag="PK112_00510"
     CDS             4267..5184
                     /locus_tag="PK112_00510"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HPC10564.1"
                     /translation="MKPDLRVTIAGVEFANPVMTASGCCGYGEELARLYPLCRLGALV
                     TKSITRAPRLGHKPPRTAETASGMLNAIGLANVGIDRFIAEKIPFLQQQDTRIIVNVA
                     GSTLEEYVEVCARLADVPRVDMIELNISCPNVSEGGIEIGSNPRLTEQTVTAVKRVFP
                     RPLVVKLSPNVTSIVPLAQAAAQGGADALSLINSLIGTAVDIETWRPRLTNNRGGLTG
                     PAIKPVALAMVDAVYNACPLPIIAIGGIATAADAVEFILCGAVAVQVGTALFVQPDTP
                     LRIVDDLSSYLERKSLSSIDELRGKVRKY"
     gene            5184..6104
                     /gene="pyrF"
                     /locus_tag="PK112_00515"
     CDS             5184..6104
                     /gene="pyrF"
                     /locus_tag="PK112_00515"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HPC10565.1"
                     /translation="MTALDKLLKAQSKNRSLVCLGLDLDPKKMPPEYSKSAKTMFDFT
                     MRIFEVTADCVCAYKPNIAFYESLGGEGVSLLMQIMHRIPEGIPVILDAKRGDIGNTA
                     THYAQALYDRYNADWVTLNPYMGYDSLRPFIERKDKGAFILCLTSNTGARDFQMLEID
                     GKPLYRIVAEKVEYWNKSRNCGLVVGATLPEQLAEIRRAAGDMPILIPGVGAQGGSVE
                     LAAVNGTDNFQKPAVINVSRSVLYASSGSDFAQKAREEVLKLNQAVNALRTGKKSEPL
                     QPPPLPPRDSAPPPPEPPAAPPASPEPTQP"
BASE COUNT         1093 a         2187 c         1836 g         1003 t
ORIGIN      
        1 ccgcgccggc atgacggcca tcggcaactg ggagacgcag gaagatatcg ccgtcgacta
       61 caccctccgc tgcggggccg ggcggtccgc cgaggtggtc ttcggaatcg aaaacaactg
      121 gaccttccag gccgggcacg ccgaggaccg ctatgtcctc atcgatggcg tgcggctgcc
      181 ggacagctat ctcgacggcg ccgccgtcca tcgggaggtg cgcacggtcc tgctcgccga
      241 cggctgccgc cgcctccagg tggggatcgt ctccgaccgg ccctacgaga tctggcgtct
      301 gccgatctgg accgtttccc agtcggagca gggattcgag cgcgtgtacc aggggaccac
      361 ggtcgtggcg gtgtaccggc tccgcctgac tgagcagccg accgtcttgt cgctgcgcct
      421 ggcggcgggg gggctcgcgg aggcttcggc gcaccgcccc cggctggact gaccccgccc
      481 ggccccttat tccgtttgat tttccgcccc cgaaccgtat cttcccgtgg tgattgacga
      541 aatcgtcagg atcatgcgcg ggagccgacg gtgtgtcgtg ctcaccggag cgggcatctc
      601 ggccgagtcg ggggtgccga cctttcgcgg caaagagggg ctgtggggga aatttcgccc
      661 cgaagagctc gcgaccatgg aggccttcct ggccaacccc gccatcgtgt gggaatggta
      721 caactggcgc cggaccgtcc tgggcagcgt ccagccgaac ccgggccact tcgccctcgc
      781 cgacatgcag gagctgttcg attctttcgt gctcgtgacc cagaatgtcg acggcctcca
      841 ccgtcaggca ggctcccgcg aggtgcttga actccacggc aatatccggc gaaacaagtg
      901 cctggactgc gcggaacccc acgccgacag cgccgacatc gacccggaag ccatcccggc
      961 ctgcccgcgg tgcgggggta aaatccgccc cgacgtcgtc tggttcggag agatgcttcc
     1021 cgaagccgtg atcgagcggg cgtttgcaga atcggcccgc gccgacgtct tcttctcggt
     1081 ggggacctcg gcgatcgtcc aaccggccgc tctgctgccg gtggaggcca aacacggcgg
     1141 cgcggctctc atcgaggtca acccggagcg gactcccctg tcgcacctgg ccgacctgca
     1201 cgtgtcggcc gcctcgggag agttcctccc ccgcctggtg gaggcttttc ggaccgcccg
     1261 cgcctgagcg gcggcgctga accggttatt ccgcccggct gtataagggg cgcggtgcaa
     1321 ggcgcgccga aacaagcgtg attcacacaa tggaccactt ccgcaccatg atagcccgag
     1381 tcgtcgtccc ggtgctcctg ctgctcgccg ccgggtgtgg cgcttactac aacacgttct
     1441 acaacgccaa gcgcgccttc aaccaggccg agagcgagcg ccgcaacacc aagccgggtc
     1501 agcccgagcg gatcctcacc ggcgactacc agaccgcgat cgaaaagtcc ctgaaagtgg
     1561 tcgagaatca ccctaactcg aagtactacg acgatgccgt gttcgtgctc ggcgtgtcct
     1621 actactacac gaaccagttc gccaaggcgg agcgccggtt ccgcgaaatc ctcgccaact
     1681 accccgacgg ccagtacgcc cgcgactcca agctgtacct ggccaaggcc atgctcaggc
     1741 aggacgcggt agccgaagcc atggacctgt tcgccgagct cttcgagagc gacctcgacc
     1801 gctcgttcaa ggcggaagca gctatggcgc tcggcaagta ccagttcgag cagggacggt
     1861 accagctcgc caaccggtac ttcctggcga tccgcgactc cctcggcgcc gatatcgaca
     1921 agcgcgtggc ccagcggtac atcgccgacg gcctcatggc gcagttccgc tataacgacg
     1981 ccctcgcggc ctacctccag gtgctcggta tggacccggc cccgcctgag cgctaccacg
     2041 ccatgttcag cgccgcccag gccgctttcc gcctgcagcg gatcgacgtc ggccaggact
     2101 acctaagcac gctggctaaa gacaatctct actttgactc cctgtcgtcg ctccggctgg
     2161 ccatggccga cggttacgag tggcagggga acctcatcaa agccgaggag atgtaccggc
     2221 tggtgtccga tgaggcggcc aaccgggacc acaaggccca ggccaacctg gcgctcggtc
     2281 tgatctacca gtacgattac gacgatctgg cgaaagccaa ggagttttat gacgcggcgg
     2341 cggccgccag ccggggttcc tcgtggggac aggaggccct gcagctctcc tcgaacatcg
     2401 gcaaactgga gaccttcgcc cgcaccgtca agctcgattc gaccaccacc caggcgcaga
     2461 tcgacgacgc cgcctacacc cagtaccagc tcgccgagtt gtactggtac cagttgtcca
     2521 agccggattc ggccatggcc gaaatgcagt acctggtgga ctcattcccg accgcctacg
     2581 atgcgccgaa agcgatgatc gccctggccc aaatggcctg gcagagcggg ccggactcgg
     2641 cccgggggga ttcgatcatc cgcgagatgc tcgggcgcta cccccattcg gatttcgtac
     2701 cggaggcgct tggcctgctc ggactggccg gcaccgaggc cgacaccggt tatgccgagt
     2761 ggtacattcg gcgagccgag aatttcgccg tcgacgacga aaacatcgac tccgccctag
     2821 ccaactatca ggtcgtgacc gaacgcttcc ccgagtccaa attctacctc caggccaagt
     2881 tcgcggggat ctggctcacc gagacgtacc ggagtccggg ggactcgtcg atcctctggg
     2941 cctaccaggc catcgccgac tcgtttccga gcaccgaatg gggccaggcc gcccgaagca
     3001 aactcaccgg ccagcctatc cgaaccctcc cgaaagccca gccggccgac acggtcaccg
     3061 gcagccgctt caacatcacc gacagctccg ccatcgacac gatggccgag ggggaatcgt
     3121 cggatacggt cagcatggac gacaagtacc tggccattat cacggcccct gacggctccc
     3181 gctgcgtcga catccccccc aacgtcggca tcatcgagac ccgcaaggag ttcgtctacc
     3241 ccagcgaggc ctatgtgtcg cggtgggaag gacagctcca ctttcaaatc cgacttgatt
     3301 tttccggaga agttcagcaa gctatcccga agacattcgc ggatgttcag gagatcaaca
     3361 ttcgcgccaa ggaaaccgtt gagtcgaccg tgttcgacat gcgcctcatc cggccggagc
     3421 tgctcgaccg ctggtttctg tatgtgttta cggtcaattt gccgagtcac ctgagatgat
     3481 gcaagccacg tgtgtcgaca cgcacgttct ctacaaacac gacctctccc acggctactt
     3541 cgcgctgact ctggcgggct atccccgcgc cggcgagtgc cgccccggcc agttcctcca
     3601 tgttcggctc cccggcagcg aagtcttctt tcgccgcccc atgtcggtcg cgggcgtcaa
     3661 cggcgaccgg ggcgagatcg acatcatctt caaagtcctc ggccgcggca cgcgcctgat
     3721 gagcgcgctg cacgacggcg acccggtcaa cctcctcggt ccgctcggca aaccgttccg
     3781 cccgcccgat gacggcagca ccgttgtgat ggtggccggc ggagtcggct ttccccccct
     3841 gctgttcctc gccgagtggc tggtgcaggg cgggtgggac ccggcgcgaa tcgaatttct
     3901 ctacggcggc cgctcgaccg gtgacatcct ctgccgcgac cggatccgcg cccttggcgt
     3961 ccgtttccac ccgaccaccg aggacggttc cctcggccgc cggggtctgg tgacggagcc
     4021 gctggaggat ctgatccgcg agcgaggcgc cgccgggctg ttcgtgtacg gctgcggacc
     4081 ggagccgatg ctcaaggcgg tcaacgacct cgggatcgcc cgccgcctcc gcggcgagct
     4141 gtcgctcgag gcccccatgc cctgcggaat cggcgtctgc ctcggctgcg tcgtcccgcg
     4201 caccaacggc gagcacgccc gcgtgtgcct cgaggggccg gtcttcgaca tcggggagat
     4261 cgccctgtga aacccgacct gcgcgtcaca atcgccggcg tcgaattcgc caaccccgtc
     4321 atgaccgcct ccgggtgctg cggctacggc gaggaactcg cgcggctcta ccccctctgt
     4381 cgcctcggcg ccctcgtcac caagtcgatc acgcgcgccc cccggctcgg tcacaaaccg
     4441 ccccgcaccg ccgaaacggc ctccggcatg ctcaacgcca tcggcctggc caacgtcggc
     4501 atcgaccgct tcatcgccga aaaaatcccg tttctccagc agcaggacac ccggatcatc
     4561 gtcaatgtcg ccgggtccac gctcgaggag tatgtcgagg tctgcgcccg gttggccgat
     4621 gtcccccggg tcgacatgat cgaactgaat atcagctgcc ccaacgtgag cgaagggggc
     4681 atcgaaatcg gcagcaaccc gcgcctgacc gaacagaccg tcaccgccgt gaagagggtc
     4741 tttccgcgcc cgctcgttgt gaagctctcg ccgaacgtga cgagcatcgt gcccctggcc
     4801 caagcggccg cccagggggg agccgacgca ctctcgctga tcaattcgct gatcggcacc
     4861 gccgtcgata tcgagacctg gcgtccccgc ctgaccaaca accggggggg gctgaccgga
     4921 ccggccatca agccggtcgc gctggccatg gtcgacgcgg tgtacaacgc ctgcccgctg
     4981 ccgatcatcg ccatcggcgg catcgccacc gcggctgacg ccgtcgaatt catcctctgc
     5041 ggcgccgtcg ccgtccaggt gggcacggcg ctcttcgtgc agccggacac accgctccgg
     5101 atcgtcgacg acctgtcgtc ctacctggag cgcaagagtc tctcttccat cgatgaactg
     5161 aggggaaagg tgaggaaata ctgatgacgg cccttgacaa gctgctcaaa gcccagagca
     5221 agaaccgctc gctggtgtgc ctcgggctcg acttggaccc gaagaagatg ccgccggagt
     5281 acagcaagtc ggccaagact atgttcgatt tcaccatgcg catcttcgaa gtgaccgccg
     5341 actgcgtgtg cgcctacaaa cccaacatcg ccttctacga atcgctcggc ggcgagggcg
     5401 tctcgctact catgcagatc atgcaccgca tccccgaggg aatcccggtg attctcgacg
     5461 ccaagcgcgg cgatatcggc aacaccgcca cccactacgc ccaggccctc tacgaccgct
     5521 ataacgccga ctgggtcacc ctcaatccct acatggggta cgattccctc cgacccttca
     5581 tcgagcgcaa agacaaaggc gccttcatcc tctgcctgac ctccaacacc ggcgcccgcg
     5641 acttccagat gctggagatc gacggcaagc ccctctaccg gattgtcgcc gagaaggtcg
     5701 agtactggaa caagagccgc aactgtggcc tcgtcgtcgg ggccactctc cccgaacagc
     5761 tcgcggaaat ccgccgcgcc gccggcgaca tgccgatcct catccccggc gtcggcgcgc
     5821 aggggggctc ggtggaactg gcagccgtca acggcaccga caacttccag aaacccgcgg
     5881 tcatcaacgt ctcccgctcc gtcctctatg cctccagcgg cagcgatttc gcgcagaaag
     5941 cgcgcgagga agtgctgaaa ctcaaccagg ctgtgaacgc cctgcggaca ggcaagaaat
     6001 ccgagccgct ccagcccccg ccgctcccac cgcgcgactc cgccccgcct ccgcccgaac
     6061 cgcccgctgc gccccccgca tcccccgaac cgactcaacc gtagcggcgg agcaggcgc
//
LOCUS       DAOOKS010000016         6030 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_15654_length_6030_cov_3.236151, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000016 DAOOKS010000000
VERSION     DAOOKS010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6030
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15654_length_6030_cov_3.236151"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="PK112_00520"
     CDS             <1..230
                     /locus_tag="PK112_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10566.1"
                     /translation="LARALVKGPEILLADQPTGNLDRENTRQLADLLTAHNRDGLTVI
                     LVTHDLPLAERLAGRVVRMHYGRLVEGTEAG"
     gene            227..1699
                     /locus_tag="PK112_00525"
     CDS             227..1699
                     /locus_tag="PK112_00525"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPC10567.1"
                     /translation="MMTVRDLVEISAGNLWRMKLRTCLTLAGVVIAIGAFVAMVSFGA
                     GAEKNVSEEYEKLGLFTTMQVYPPAADSLGDSVPPPPLTDSVVARLARLPGVRLAYPY
                     DAFSVSVRLDTLELSAEAQVLPPAAVATRLYSRLVAGRRPDANGSREAMVTREFLEDH
                     ALGPADSILGRSIAVSVSSSTLDSAVVHVVRQESRRVEQFIDAFDFDSLDNVGYLRRT
                     ATGEFNAAARSFLEGYLGRPMITTDTLTIVGVIDGPQGRRLRVKPLIIPEQSARRFAA
                     AKSGPDPAELLAAFQSGRVGNLFGGDTAAPDAYSRVTLDLEPGTGYAALSDSIRAMGY
                     ETFSYLEQFAEIQRFFLYFDLMLGVVGMIALVTASLGIVNTMVMSILERRREIGVLKS
                     LGADDRDVWLQFLAESGVIGSVGAVLGILLGWVVTRIASFVAVRVMENQGMTAVELFD
                     LPLWLIAVALLFGTAVSVAAGLYPAARAARVNPVEALRNE"
     gene            1726..2721
                     /locus_tag="PK112_00530"
     CDS             1726..2721
                     /locus_tag="PK112_00530"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HPC10568.1"
                     /translation="MDRRSREAFAAGRTALLSEAAREWDFEPRTAAELDGFENHIFEV
                     ARGGRPFVLRLCHSLHRTAALVCAELHWLDFLARRGLPVAAPVPGRGGALARVIPAAD
                     SFFVASLCAKAPGRRPLRSDYSDRFFENWGELVGRIHRVTREYLPLAPPVRRFHWHED
                     PTLDPAPYRPAVDDDVLARFARIVAQLRALPVDRTAYGLIHNDLHPGNFFIDGDCLTA
                     FDFDDCHYQFLVNDVAMALFYALPLPARPRTAEAFAETFLPPFLRGYRRHAALGPEDL
                     RRLPMFLKLRELDLYSQIVLHLGADVGGWPGAYLEGRRDRILADRPVVDLDFAEF"
     gene            complement(2750..3700)
                     /locus_tag="PK112_00535"
     CDS             complement(2750..3700)
                     /locus_tag="PK112_00535"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPC10569.1"
                     /translation="MPTKPSRPGPAGALAAQRRTGYLLALISAVGGGAATATGKWNLV
                     HLSPLAMNALIFSLATVMLSAWVLPGYGPARVFRQTRRAWFWILLFSLSSWFAVLLVW
                     AGVQKMDPSLASFLNRSEVLVAIALGILFLGERLSRIETLGVLLSLAGIALMRLTLRG
                     EYSVGFGLVLLGSIGFGLTEFISKHAVRHVDPLILTYIRNGLLALGYWAVLFAAGGDL
                     AGLAQVWPGVIALALFGPLVSRLFYLQALARLELTKVAVISQSQPVVVMLIALALLHQ
                     LPTAREALGGLLLLIGCVIMAAAHRRPWARFGQQADTRAG"
     gene            complement(3706..4530)
                     /locus_tag="PK112_00540"
     CDS             complement(3706..4530)
                     /locus_tag="PK112_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10570.1"
                     /translation="MAARHYAILSISAAALLPAACGSDQDRFPTITYEMKTIERRFGT
                     EGDTVNGFAYLNVTYPEVTAASERGVADSVSARVWQFLLRPMAGAGTFETVDDMAKEF
                     IRNFKDRPDPARTASYGWTWLRKARVETDTLGILGVSLVETRYQGPEDQHAERRYVNF
                     DARTGRLLALSDLLIPGGDTLLNRTAEQAFRRARRLDLVESLAAAGFAFDSTAGFRHN
                     GNFLITPKGLAFYVNRGEAGKEELGPTGYTVPYTDLAALARPDGPLTPFVAPPPGL"
     gene            4735..>6030
                     /locus_tag="PK112_00545"
     CDS             4735..>6030
                     /locus_tag="PK112_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018465948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HPC10571.1"
                     /translation="MTDAPSEPPDEIVPRKPPPVLSDDLTRRIAEQVLSGERVSRWQR
                     LLPFLGPAFIASVAYVDPGNFATNIQGGAEFGYRLLWVIVFSNLMAMLLQTLSAKLGI
                     ATGYNLAEHCRREFPTWVAWGMWVLMEAVAMSTDLAEFLGAALGFYLLLGIPLIWGAV
                     LTGIATFLILGLERRGFRRLEAVITAMVAVIVFAYVVESIMDRPDWGLVACHAVVPSL
                     PGPEAVLLAAGILGATVMPHAIFLHSALTQGRIQVRGAAQLQRLFRFQIIDVTVAMGL
                     ASLVNMAILIMAAATFHRHGLSEIASIEEAHRTLEPLLGSAASGVFAVSLLASGLSSS
                     TVGTSAGQVIMQGFLRRHIPVWVRRLVTMAPALIVIALGLDPTRTLVISQVVLSFGLP
                     FAVVPLVLFTARRSIMGVLVNRKPTTVAAAIVASLIIGLN"
BASE COUNT          900 a         2151 c         1950 g         1029 t
ORIGIN      
        1 cgctggcccg cgcgctggtg aaaggcccgg agattctgct tgccgatcaa ccgaccggca
       61 acctcgaccg cgagaacacg cggcagttgg cggacctcct gaccgcacac aatcgcgacg
      121 ggctgaccgt gatcctcgtc acccacgatc tcccgttggc cgagcggctc gccggccgcg
      181 tcgtgcggat gcactacggg cggctggtcg aaggaaccga ggccggatga tgaccgtccg
      241 cgacctcgtc gagatctccg ccggcaatct ctggcggatg aaactgcgga cttgcctcac
      301 cctggcgggt gtcgttatcg ccatcggcgc cttcgtcgcc atggtctcgt tcggggccgg
      361 cgcggagaag aacgtctccg aggagtatga gaaactcggt ttgttcacga ccatgcaggt
      421 ctacccgccg gcggccgaca gcctcggcga ctcggtgccg ccgccgccgc tgaccgactc
      481 ggtcgtcgcc cgcctggcgc gtctgccggg cgtgcgcctc gcttatccct acgacgcctt
      541 cagcgtctcc gtccgcctcg acacgctcga gctctcggct gaggctcagg tgcttccgcc
      601 ggcggccgtc gcgacccgtc tgtattcccg tctcgtcgcg gggcggcgac cggacgccaa
      661 cggctcgcgc gaggcgatgg tgacgcgcga gtttctggaa gatcacgcgc tcggacccgc
      721 cgactcgatc ctcggtcgat cgatcgccgt ctccgtctcg tcctcgacgc tcgacagcgc
      781 cgtggtccac gtcgtccgcc aggagagccg gcgcgtcgaa caattcatcg acgcctttga
      841 cttcgattcc ctcgacaacg tcgggtacct tcggcggacg gcgaccggcg aattcaacgc
      901 cgcggcgcgg agctttctcg aggggtatct cgggcggccg atgattacca ccgacacgct
      961 gaccattgtc ggcgtgatcg acggcccgca gggccgccgg ctccgggtca aacccctcat
     1021 cattccggag caaagcgccc ggcgcttcgc cgcggccaag agcggcccgg acccggccga
     1081 actgctggcc gccttccaga gcgggcgcgt cggcaacctc ttcggcggcg acacagcggc
     1141 gcccgacgcc tacagccgcg tcacgctcga cctcgaaccg gggaccggct acgccgccct
     1201 gagcgacagc atcagggcga tgggctacga gaccttcagc tacctcgagc agttcgccga
     1261 gatccagcgc ttcttcctct atttcgacct catgctcggg gtggtgggga tgatcgcgct
     1321 ggtaaccgcc tccctgggga tcgtcaacac catggtcatg tcgatcctcg aacgccgccg
     1381 cgagatcggc gtgctgaaat cgctcggcgc cgacgaccgc gacgtctggc tccagttcct
     1441 cgccgaatcc ggcgtcatcg gctcggtcgg cgccgtcctc ggcattctcc tcggttgggt
     1501 tgtgacccgc atcgcttcgt tcgtggccgt gcgggtgatg gagaaccagg gaatgacggc
     1561 ggtggaattg ttcgatctcc ccctgtggtt gatcgcggtc gcgctcctgt tcggcacggc
     1621 ggtgagcgtc gccgccggcc tttaccccgc cgcgcgggcc gcccgcgtca atccggtcga
     1681 agccctccgc aatgagtgag cccgctccgg agaagggaga cgtctgtgga ccgacgatcc
     1741 cgtgaggcat tcgcagccgg ccgaaccgcg ctgctttcgg aagcggcgcg cgagtgggat
     1801 tttgagccgc gcacggccgc cgaactggat ggcttcgaga accacatctt tgaggtcgcg
     1861 cgcggcgggc gtccgttcgt gctccgtctg tgccactcgc tccaccgtac ggccgcgctc
     1921 gtctgcgctg aattgcactg gctggacttc ctggcccggc gcgggttgcc cgtggccgcg
     1981 cccgttcccg gccgcggcgg cgccctcgcc cgcgtgatcc ccgccgccga ctccttcttc
     2041 gttgcctcac tctgtgcgaa agcccccggc cggagaccgc tccggagcga ctacagcgac
     2101 cgcttcttcg aaaactgggg ggagctggtc ggccggatcc accgcgtcac ccgggagtac
     2161 ctgccgctcg cgccgccggt ccggcgcttc cactggcatg aggacccgac gctcgacccg
     2221 gcgccctatc gcccggccgt cgacgacgac gtcctcgccc gcttcgcccg gatcgtggcg
     2281 cagctcaggg cgctgccggt cgatcgtacg gcctacggtc tgatccacaa cgaccttcac
     2341 cccggcaatt tcttcatcga cggcgactgt ctgacggcgt tcgatttcga cgattgccac
     2401 taccagtttc tggtcaatga tgtagcgatg gccctgtttt atgccttgcc cttgcccgct
     2461 cggccgagga ccgccgaggc gttcgcggag acgttccttc cgccgttcct ccgcggttac
     2521 cgccgtcacg ccgcgctggg gccggaggac ctgcggcgtc tcccgatgtt cctcaaactc
     2581 cgcgagctcg acctctacag ccagattgtg cttcacctcg gagccgacgt cggcggctgg
     2641 cccggcgcgt acctcgaggg gcggcgcgac cgcatcctcg ccgatcgccc ggtcgtcgac
     2701 ctggatttcg cggaattttg acccgggccc cggtgggcgg cagcccgtcc tatccggccc
     2761 gggtgtccgc ctgttgcccg aatcgcgccc acggcctccg atgcgcggcg gccatgatga
     2821 cgcagccgat caggagcagc aacccgccga gcgcctcccg cgccgtcggc aactggtgca
     2881 agagcgccag cgcgatgagc atcaccacca ccggctggct ctggctgatc accgccacct
     2941 tggtgagttc caaccgggcc agcgcctgca ggtagaacag ccgggagacg agcggcccga
     3001 acagcgccag ggcgatcacc ccgggccaca cctgggcgag tcccgccaga tcgccgcccg
     3061 cggcgaacag caccgcccag taccccagcg cgagcagtcc gttgcggatg tatgtcagga
     3121 tcagcgggtc gacatgccgc accgcgtgct tggaaatgaa ctcggtgagg ccgaaaccga
     3181 tcgaccccag gagcacgagc ccgaacccga ccgagtattc cccccgcagc gtcagccgca
     3241 tgagcgcgat cccggccagc gacagcagca cccccagcgt ctcgatccgg ctgagccgct
     3301 ccccgaggaa cagaatgccg agcgcgatcg ccaccagcac ctccgaccgg ttgaggaagg
     3361 aggccagcga tggatccatc ttctggacgc cggcccacac caggaggacg gcgaaccagg
     3421 acgagaggct gaacaggaga atccagaacc aggcgcgccg ggtctgccgg aaaacccgcg
     3481 ccggaccgta gccgggcagc acccacgccg acaacatcac cgtcgccagc gagaaaatca
     3541 gcgcgttcat ggccaggggg gagaggtgca caaggttcca tttcccggtc gccgtggccg
     3601 ccccgccccc aaccgccgaa atcagcgcca aaaggtaacc ggtgcgccgt tgcgccgcaa
     3661 gcgcgccggc cggtccgggg cggctcggct tggtcggcat gacggctaca gccccggtgg
     3721 gggcgcgaca aagggggtca ggggaccatc ggggcgggcg agcgccgcca ggtcggtata
     3781 ggggaccgtg tagccggtcg ggcccaattc ctccttgccc gcctcgccgc ggttgacata
     3841 gaaggccaag cctttcgggg tgatgaggaa gtttccgttg tgacggaaac cggccgtgga
     3901 atcgaacgca aacccggccg cggccagcga ctccacgaga tcgagtctgc gggcgcgccg
     3961 gaacgcctgc tcggcggttc ggttgagaag tgtgtcgccg ccgggaatca gcaggtcgct
     4021 cagggcgagc agccggcccg tccgggcgtc gaaattgaca taccgccgct ccgcatgctg
     4081 gtcctccgga ccctggtacc gggtctccac caggctcact cccaagatgc cgagcgtgtc
     4141 ggtctccacc cgggctttcc gcaaccacgt ccagccgtag ctggctgtcc gcgcgggatc
     4201 cggccggtcc ttgaaattcc ggatgaactc cttggccatg tcgtcgaccg tctcgaacgt
     4261 gcccgctccc gccatcggcc gcaggaggaa ctgccagacg cgcgcggaga ccgagtcggc
     4321 gacccctctc tccgaggctg ccgtgacctc cgggtaggtc acgttgaggt aggcgaaacc
     4381 gttgaccgtg tctccctcgg tgccgaaccg ccgctcgatc gttttcatct catacgtgat
     4441 tgtggggaag cggtcctggt cggaaccgca ggccgcgggc aggagggccg ccgcgctgat
     4501 cgagagaatc gcgtaatgtc gcgccgccat gggtgatacc tcgagttggt tccgccaagg
     4561 tagcgcaaac tcccggtaaa ttcaacttct ttgtgggaac cgccgatggg gggtgcggcg
     4621 gctgcggtgc gaagagaatc ttgcgcctgc gggagactcc tgcgtacgtg ttgtcggcgg
     4681 gcggcgcagc cccgaaagta acgcgcccga cccgatttcg acagcgagcg aaatatgacc
     4741 gacgcgccat ccgaaccgcc cgacgagatt gtcccgagga agccgccgcc cgtgctctcg
     4801 gacgacctga cgcggcggat cgccgagcag gtgctgtcgg gggagcgcgt gtcccgctgg
     4861 cagcggttgc tgccgttcct cgggccggcc ttcatcgcca gcgtcgccta cgtcgatccc
     4921 ggtaatttcg ccaccaatat ccagggcggc gccgagttcg gctaccgcct cctctgggtg
     4981 atcgtcttct ccaatctcat ggccatgctg ctccagacgc tctccgcgaa actgggcatc
     5041 gccaccggct acaacctcgc cgagcactgc cgccgcgagt ttccgacctg ggtcgcctgg
     5101 gggatgtggg tgctcatgga agcggtcgcc atgtccaccg acctggccga attcctcggc
     5161 gccgccctcg ggttctacct cctgctcggc atcccgctca tctggggcgc ggtgcttacc
     5221 gggatcgcga ccttcctcat tctcggtctg gagcgccggg ggttccgccg cctggaggcg
     5281 gtcatcacgg ccatggtcgc cgtcatcgtc ttcgcctatg ttgtggaatc gatcatggac
     5341 cggccggact gggggctggt ggcctgccat gcggtcgtcc cgagccttcc cggcccggag
     5401 gcggtgttgc tggcggccgg gattctcggc gcgaccgtga tgccgcatgc gatttttctc
     5461 cattcggccc tgacccaggg ccgcatccag gttcgcggcg ccgcccagct gcagcggctc
     5521 tttcgtttcc aaatcatcga cgtcaccgtc gccatggggc tcgccagcct cgtcaacatg
     5581 gccatcctca tcatggccgc ggccactttc catcgccacg gcctgtccga gatcgcctcc
     5641 atcgaggagg cccaccggac tctcgaaccg cttctcggca gcgccgccag cggcgtcttc
     5701 gccgtgtcgc tgctggcctc cgggttgtcc tcctcaactg tcggtacgag cgccggccag
     5761 gtgatcatgc agggcttcct gaggcgccac atcccggtct gggtgcgccg gctggtgacg
     5821 atggcgccgg cgctgatcgt catcgccctc gggctcgacc cgacgcggac gctggtcatc
     5881 agccaggtgg tcctgagttt cggcctcccg ttcgccgtcg tgccgctcgt gctgttcacc
     5941 gctcgccgct cgatcatggg cgtgctggtg aaccggaaac cgactaccgt cgcggccgcg
     6001 atcgtggcgt cgctcatcat cggcctgaac
//
LOCUS       DAOOKS010000017         5842 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_16393_length_5842_cov_3.234837, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000017 DAOOKS010000000
VERSION     DAOOKS010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5842
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16393_length_5842_cov_3.234837"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1606)
                     /locus_tag="PK112_00550"
     CDS             complement(<1..1606)
                     /locus_tag="PK112_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10572.1"
                     /translation="MIDPLINWPTYYGQQNTGLVRATFGDNGSFGWSFLRRTMGIPGW
                     PEAGFEVPVSSGVDYLFGGAVWIGGVVGSDTLVSTGSEGWVNVREFFPDDLAGAVDEL
                     FGPADFAMRAEFTDTSVARVSVTDEFDSRLHLPLDVRITNRSHAWRTSPADAAVVYDL
                     VVTNIGVEAVHDGYIGFYFDSDVCGDCDANSGFADDLSGSLREQGIGYVIDNDGDSLP
                     GETPRAFCFKLLHSSVPLPDTSFNWWASSAIAELDFGPRMKDRPGDPYRDFGTGGTGT
                     AVGDANRYYLMRHQEWDYDQVWCHSIGPDDTLWWQPPLSLAYDLADGFDARFLMSFGP
                     FDLAPGQSERFIIATFTTDSIHTLPGNAANLPGDPAAYLANLDFTSMIRNANVADSLA
                     AILVDPLQPPAGLQVRATSGNDVTIGWDGSCLPEVTGHVVYLSVVDPASLPYPGVAPP
                     WLEMTELEIEATPGPEQSAWTLPNLATDRFYLASVSYRSASGTGEPAHPITVWAGERA
                     EAPEFVGSEYVFVAPGDNIPLSWTTLS"
     gene            1921..2451
                     /locus_tag="PK112_00555"
     CDS             1921..2451
                     /locus_tag="PK112_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10573.1"
                     /translation="MMDSYVAWVTARPLLSAALQFALLGTLGEIVSVSIRRGRPALPG
                     TWWETLAKVPAWAVLGIVIKYGFTGMKGFTAALLDHHFLPAACGAGLGWAFAVSVLTN
                     LLFGPQMMFFHRLEDNLILRRWDMTGLTTAWWTLLWFWIPAHTLTFSLPTEYQIGLAA
                     LWSFVLGIILSFAKGR"
     gene            2455..2871
                     /locus_tag="PK112_00560"
     CDS             2455..2871
                     /locus_tag="PK112_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005544293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPC10574.1"
                     /translation="MVIEYVDSAAGLAPDRLTGFFAGWPNPPSPEMHRRILRRSDCVV
                     LAIDPSAGRVVGFINALTDRVLSAYIPLLEVLPEYRGQGIGSELVRRLLKRLSGLYMI
                     DLMCDPSVQPSYERLGFVRAAGMSRRDFSAQGGKPD"
     gene            complement(2879..3436)
                     /locus_tag="PK112_00565"
     CDS             complement(2879..3436)
                     /locus_tag="PK112_00565"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPC10575.1"
                     /translation="MRAGKSIATLRLELIPATPSLLAADVARSARLAEELGAVVPPDW
                     PPSELAEVLPRLLSALERRPDLRGWWAYYWVASGEGRGPRTLIGSGGFHGRPDAEGCI
                     EIGYGVLPDFQGRGYATEAVAALVAWALRHRTVTMVFANTGADNQPSIRLLERLGFLR
                     AGIGAEPWTVRFEVDRRRFRRPMPR"
     gene            complement(3455..4264)
                     /locus_tag="PK112_00570"
     CDS             complement(3455..4264)
                     /locus_tag="PK112_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014587468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HPC10576.1"
                     /translation="MTAGPGRPLRGAVIAALLAVLPAACGSSQKQPAPPAKIAAYRVK
                     VIRTYPHDSLAFTQGLAYEGGYLYEGTGKPGASSVRRVRLETGAVLQRRDLPAPHFGE
                     GIALVGDRLVQLTWTSRTGFVYDRATLAPLSEFRYLTEGWGLTYDGIRLIMSDGSATL
                     QLLDPQTYEYCSFLAVRADGEPVERLNELEYVDGEIFANVWPGDRIARISAATGQVTG
                     WIDASGLLAPEDYGDSTDVLNGIAWDAEHRRLFLTGKCWPKLFEVELEPVE"
     gene            complement(4261..5085)
                     /locus_tag="PK112_00575"
     CDS             complement(4261..5085)
                     /locus_tag="PK112_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014587468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HPC10577.1"
                     /translation="MMRTACYMSRAGCALAALSVGAALSAGCSKSTDAEPPPAPDSTR
                     TYTYTVIDSFPHDSTAYTQGLAFGPEGYLYEGTGLYGGSSLRRVELTTGRVDTMIGLA
                     ALYFGEGIALLGNRIVQLTWRENSAFVWNRASFAREGQFSYAGEGWGLTFDGSRFIMS
                     NGGSALQFRDTATFEIRRSAQVRDDDGPVDRLNELEYIDGEVWANIWLTDWIARIDPD
                     DGRVTGYVDLTGLLSRTAHPTAEVLNGIAWDSAGARLFVTGKYWPWLYQIEVREAP"
     gene            5218..>5842
                     /locus_tag="PK112_00580"
     CDS             5218..>5842
                     /locus_tag="PK112_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HPC10578.1"
                     /translation="MMSRPLRTACALFLLLALLGPATGPAAQENITYQTPPQAIVDIV
                     DAPRTPRLSLGPDRQNLLLLEYPALPPLEEVAQPELRLAGERINPRTNGPSRGGYYSS
                     MKLRTLDDRTEYPITGLPSPVRITDIEWSPDGSRVAFACTIEDRIELWLLDLAARTAR
                     RLSERPLNAVFGSPFTWFSDSRTLLVRLSPIVGEEPNKQRPAEGPNVQ"
BASE COUNT         1007 a         1968 c         1856 g         1011 t
ORIGIN      
        1 aagaaagtgt cgtccagctc agcgggatgt tgtcgcccgg cgcgacgaac acgtactcgc
       61 tgccgacaaa ttccggcgcc tcggcgcgct cgccggccca gaccgtgatc ggatgggcgg
      121 gttcaccggt cccggaggca ctccggtagg aaaccgaggc gagatagaaa cgatcagtcg
      181 caaggttcgg gagcgtccaa gcggactgct ccggcccggg cgtggcctcg atctccaatt
      241 cggtcatttc cagccagggc ggggcaaccc cgggataggg aagagaggcg gggtcaacca
      301 ctgacaggta aacgacatgg ccggtcacct cgggcaggca ggagccatcc caaccgatgg
      361 taacgtcgtt cccgctggtc gctctcacct gcaagccggc cggcggctgc agcgggtcaa
      421 caaggatcgc ggccagtgaa tcggccacgt tggcgttgcg gatcatgctg gtgaagtcca
      481 ggttcgccag ataggctgcc gggtcccccg gcaagttggc ggcattccca gggagcgtgt
      541 ggatcgaatc ggtcgtgaag gtggcgatga tgaaccgctc gctctggccc ggcgccagat
      601 cgaacggccc gaacgacatc aggaatcggg cgtcaaagcc gtccgccagg tcgtaggcca
      661 gggacagcgg cggctgccac cagagggtat catccggacc aatggagtga caccatactt
      721 ggtcgtagtc ccactcctga tggcgcatca ggtagtaccg gttggcatcc ccgacggcgg
      781 tgcctgtccc gccggttccg aaatcccggt acgggtcgcc cggccggtcc ttcatccgcg
      841 gaccgaaatc cagttcggcg attgcgcttg aggcccacca gttgaagctc gtgtcgggca
      901 gcggcacgga cgagtgcagc agcttgaaac aaaacgcccg gggcgtctca cccggcaatg
      961 agtccccgtc gttgtcaatg acgtagccaa ttccctgttc gcgcagagac ccggagagat
     1021 catccgcgaa acctgagttg gcgtcacagt caccgcagac atccgagtcg aagtagaacc
     1081 cgatataacc gtcgtgaacc gcctcgacgc cgatgttggt cactaccagg tcatagacga
     1141 cagccgcatc ggccggcgac gtgcgccagg catggctgcg gttggtgatc cggacatcca
     1201 gcggcaggtg caggcgggag tcaaactcat ccgtcacgga aacgcgggcg acgctggtat
     1261 cggtgaattc agcccgcatc gcaaagtccg ccggaccgaa cagctcatcg actgcgcccg
     1321 cgagatcatc ggggaagaac tcccgcacgt taacccaacc ttcagatcct gtggacacca
     1381 gcgtgtcgct ccccaccacc ccgccgatcc agacggcgcc tccgaagagg taatcgaccc
     1441 cgctgctcac cggtacttcg aaacctgcct cgggccaacc gggaatcccc atcgtacgcc
     1501 gcaggaagga ccacccaaag gagccattat ccccgaatgt cgcgcgcacc agtcccgtgt
     1561 tctgctggcc atagtacgtc ggccagttga tcagcggatc aatcacagtg tctgcggcgt
     1621 acggtgcaac atacagcgcc cggttctgcc gcccctcacc ggcgtccggt ttttgatctc
     1681 gggcctggcc tgcagtccag agaaaggctg tggccagcgc ccacgctgtc acaataccag
     1741 acctggtcat cctcgttcct cctcgattga atttgatgcc tgacaatagc gcgccatgcc
     1801 tgtgctgtca atcaatgacg gcagtcgccc ggtgtctgtt taatctctcc tgttttttct
     1861 tgaactcacc gcctgcggcc gatatgctgt cgtcgacatc aacctaccgc ctttggagcg
     1921 atgatggatt cgtacgtcgc ctgggtcacg gcccgcccgc tgctctcggc cgccctgcag
     1981 tttgccctgc tgggcacgct tggtgaaatc gtctcggtca gcatccggag aggccgcccc
     2041 gccctccccg gaacgtggtg ggaaacgctc gccaaagtcc cggcctgggc agtgctcggc
     2101 attgtcatca agtacgggtt cacggggatg aagggattta cggcggccct gctggaccat
     2161 cattttctgc ccgcggcgtg cggcgccggg ctcggctggg cgttcgccgt ctccgtgctc
     2221 accaacctcc ttttcgggcc gcagatgatg ttcttccacc ggctcgagga caatctcatc
     2281 ctccgccgct gggacatgac cgggctaacc accgcgtggt ggacgctcct gtggttctgg
     2341 atcccggctc acacgctgac cttttcgttg ccgaccgagt accagatcgg cctggcggcg
     2401 ctctggtcgt tcgttttggg gatcattcta agctttgcca aggggaggtg atccatggtg
     2461 atcgagtatg tggattcggc ggccggtctc gcgcccgacc gtctgaccgg ctttttcgcc
     2521 ggctggccga atcctccgtc gccggagatg caccggcgca tcctccggcg gagcgactgt
     2581 gtcgtgctcg cgatcgatcc atccgccggc cgcgtcgtcg gcttcatcaa cgctctcacc
     2641 gaccgcgtcc tctccgccta cattccgctt ttggaggttc tccccgagta ccggggacag
     2701 ggcatcggaa gcgagttggt ccggcgactg cttaagcggc tgtcggggct gtatatgatc
     2761 gacctgatgt gcgatccgtc agtccagccg tcttatgagc gtctgggatt cgtgcgcgcg
     2821 gcggggatga gccggcggga tttttcggcg caaggcggaa aaccggatta gcaggaggct
     2881 accggggcat ggggcggcga aagcgccgcc ggtcaacctc gaaacggact gtccagggct
     2941 cggccccgat gcctgcgcgg aggaatccga gccgctccag gagacggatg gacggctggt
     3001 tgtccgctcc ggtgttggcg aagaccatcg taaccgtgcg atggcgcagc gcccaggcga
     3061 ccagggcagc caccgcctca gtcgcgtatc cgcggccctg gaagtccggc aacacgccgt
     3121 agccgatttc gatgcaccct tcggcatcgg gccggccgtg gaatccgccg ctgccgatca
     3181 gggtgcgcgg tccgcgtccc tcgcctgaag ccacccagta gtaggcccac cagccgcgca
     3241 ggtccggacg gcgctccaga gccgacagca ggcgcgggag cacctcggcc agttcggaag
     3301 gcggccagtc gggcggcacg accgccccca gttcctccgc caatcgggcc gaacgggcga
     3361 catcggccgc cagcaacgag ggcgtggccg ggatcagttc cagccgcagc gtcgcgatcg
     3421 acttaccggc gcgcacagct cacactcctt gcagtcattc cacgggttcg agttctactt
     3481 cgaacagctt gggccagcac ttgccggtga ggaacagacg acggtgctcg gcatcccagg
     3541 cgataccgtt gaggacatcg gtgctgtcgc cgtagtcctc gggcgcgagc agcccggagg
     3601 cgtcgatcca gccggtgacc tgtccggtgg cggcgctgat acgggcgatg cggtcgccgg
     3661 gccacacgtt ggcgaaaatc tcgccgtcga catactccag ctcgttgagc cgctcgaccg
     3721 gttcgccgtc ggcccgcacg gccaggaaac tgcaatactc gtacgtctgc ggatccagaa
     3781 gctgcagcgt cgcgctgccg tcgctcatga tcagacggat cccgtcgtac gtgagccccc
     3841 agccttcggt gagatacctg aactcggaca gaggcgcgag cgtcgcccgg tcgtacacga
     3901 atccggtgcg ggaggtccag gtgagctgaa ccaggcggtc tccgaccaga gcgattccct
     3961 cgccgaaatg gggcgccggc agatcgcggc gctgcagcac cgcaccggtc tcgagccgga
     4021 cccggcgcac gctcgacgcg ccgggtttcc cggtcccctc gtacaagtag cctccttcgt
     4081 aggccaggcc ctgcgtgaaa gcgagactgt cgtgcggata cgtgcggatc actttcaccc
     4141 ggtaggcggc gatcttcgcc ggcggcgccg gctgcttctg cgacgatccg caggcggccg
     4201 gcagcaccgc cagcaaggcg gcgatgaccg cgccgcgcag cggcctgccg ggcccggccg
     4261 tcacggcgcc tcccgcactt caatctggta gagccagggc cagtatttgc cggtgacgaa
     4321 gagccgcgcc ccggccgaat cccaggcaat gccgttgagg acctccgccg tcgggtgggc
     4381 agttcgcgag agcagcccgg tcaggtcgac gtagcccgtc acccggccgt cgtccgggtc
     4441 aatccgggcg atccaatcgg taagccagat gttggcccag acctcgccgt cgatatactc
     4501 cagttcgttg aggcggtcaa ccggcccgtc gtcgtcgcga acctgggcgc tccgccggat
     4561 ctcgaacgtg gccgtgtcac gaaactggag agccgagccg ccgttgctca taataaaccg
     4621 gctgccgtcg aaggtcagcc cccagccctc gcccgcatag ctgaactgac cttcccgcgc
     4681 gaacgatgcg cggttccaca cgaacgccga gttttctctc caggtgagtt ggacgatgcg
     4741 attccccagc agggcaatcc cctcgccgaa atagagcgcg gcgaggccga tcatcgtatc
     4801 gacccggccg gtcgtcagtt cgacccggcg cagcgacgag ccgccgtaca aaccggtccc
     4861 ctcgtacagg taaccctcgg gaccgaaagc gaggccctgg gtgtacgcgg tcgagtcgtg
     4921 ggggaagctg tcgatcaccg tgtacgtgta agtgcgcgtg gagtcggggg ccggcggcgg
     4981 ttcggcatcg gtcgatttcg agcagccggc ggagagggcc gcgcccacgc taagcgcggc
     5041 cagcgcgcac ccggcgcggc tcatgtagca cgcagttcgc atcatccgct ggccaaacta
     5101 ccgggcgcgg cctcaagacg caacgacaat tccgacggtc ttgtgcccgg ccgccggccg
     5161 tgttatactc ccggcgtgac cagcaacatg tgtgctcttt tctacaggag tctcgccatg
     5221 atgtctcgcc cgctgcgaac cgcttgcgcg ctcttccttc ttctggccct gctcggcccg
     5281 gcgacggggc cggcggccca ggagaacatc acctaccaga cgccgccgca ggcgattgtc
     5341 gacatcgtcg acgcaccccg gacgccgcgg ctgtcgctcg gccccgacag acagaacctt
     5401 ctcctcctgg agtaccccgc cctcccgcct ctcgaggagg tggcccaacc ggaactccgg
     5461 cttgccggcg agcgcatcaa cccccgcacc aacggcccca gccgcggcgg gtattacagc
     5521 agcatgaagc ttcggacgct cgatgatcgg accgagtatc ccatcacggg tctgccctct
     5581 cccgtccgca tcaccgacat agagtggtct ccggacgggt cacgggtcgc ttttgcctgt
     5641 acgatcgagg atcggattga gttgtggctg ctcgacctgg ctgcacgcac cgcccggcgg
     5701 ctttcggagc ggcccctgaa cgccgtgttc ggcagcccgt tcacatggtt ctcggacagc
     5761 cggacgctgc ttgttcggct ctccccgatc gtcggggagg aaccgaacaa gcagcgtccg
     5821 gctgagggcc cgaacgtcca gg
//
LOCUS       DAOOKS010000018         5731 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_16828_length_5731_cov_3.308844, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000018 DAOOKS010000000
VERSION     DAOOKS010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5731
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16828_length_5731_cov_3.308844"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..1584)
                     /gene="fliF"
                     /locus_tag="PK112_00585"
     CDS             complement(22..1584)
                     /gene="fliF"
                     /locus_tag="PK112_00585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HPC10579.1"
                     /translation="MEQVRQFFRNLAAWVGRLSPGQVMMLLGIAAGTIVGTILLVGWV
                     NTVTYARLYSDVDEAEAGEVVAYLNDNKIPYELSDGGRTIAVPSDQVYKTRIALASEG
                     LPRNGNIGYSIFDKNNLGMTDFLQNLNFRRALEGELTRTIMQLSEVQAARVHIVMPSD
                     RLFKQDQKQATASVVLKLRSGGLTKQQINGISHLVAASVEGLRPENISIVDYDGNLLS
                     SGQPTDELAGLTASQLDVRKQAETYLEHKAQSMLDDVLGPGRSVIRVTAELDFNQTER
                     TSETYDANQPAVRSEERTKQTNASTDKAAEASESQEQGSTETTITNYELNKTVEHIIN
                     GIGSIKRLSVAVMVDGVYTPAAAEDGGAAMSYQPRSQEELDRLAAIVRNAVGFDSQRN
                     DQIEMVNIAFDRRDLIEDQQALDSMYRRDFYLEIAKKVGLVLLALLVFLYLRRKVSRL
                     FQSIGAMAAAPKRTPARPLTPGAAPEAAAEEGPRVIPEQRRTRLVDEMQDAARKQPEE
                     IARAIKTMMSDV"
     gene            complement(1646..1966)
                     /locus_tag="PK112_00590"
     CDS             complement(1646..1966)
                     /locus_tag="PK112_00590"
                     /inference="COORDINATES: protein motif:HMM:NF014143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HPC10580.1"
                     /translation="MTAPVNGLTRLVPGLVARLEPTGKTVPLPEMKAEDGFGEMLSDF
                     VSGVNSLQYESAQIQQAFLEGEPVELHQVMVKAEEAGVAAELLIEIRNKLVEGYQQLI
                     QMPL"
     gene            complement(1968..2282)
                     /locus_tag="PK112_00595"
     CDS             complement(1968..2282)
                     /locus_tag="PK112_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10581.1"
                     /translation="MKINPTAIQSYQQLVQRQPQRPAEQARPADAARELTIAPQAAAS
                     GSDLAVKAPEGSYADFLTEAERAALELLFQRFGDSGRFGAALGGEAAESRVLGKLIDV
                     KV"
     gene            complement(2300..2794)
                     /gene="flgC"
                     /locus_tag="PK112_00600"
     CDS             complement(2300..2794)
                     /gene="flgC"
                     /locus_tag="PK112_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HPC10582.1"
                     /translation="MAGILGAIEIASRGLSVQRAKMNATAENIANAETTQTPEGGPYR
                     RKRVQVREDETGVSFRQAMRRAGVSLERTNTRHFPARGVCVQSEVEVPDVDMKEIRDP
                     QSSYRLIHDPEHPDADEQGYVKMPDIEVVNEMVDMMAAARAYEANTVTIAAAKQMAKD
                     ALDI"
     gene            complement(2806..3213)
                     /gene="flgB"
                     /locus_tag="PK112_00605"
     CDS             complement(2806..3213)
                     /gene="flgB"
                     /locus_tag="PK112_00605"
                     /inference="COORDINATES: protein motif:HMM:TIGR01396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HPC10583.1"
                     /translation="MKIADFLFKSTGVHRFEAYLDVSSFRHKLISSNVANVSTPGYGA
                     RDIDFQAEYTRLTGETGRVAGELTHPAHLPLGAHRDREPDIEETRPKPDELNAVDIDR
                     EVSQMAQNELQFTVAARLLQKKFEGLRRAIAGR"
     gene            complement(3392..4138)
                     /locus_tag="PK112_00610"
     CDS             complement(3392..4138)
                     /locus_tag="PK112_00610"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HPC10584.1"
                     /translation="MRRFRRRVEDKENLDRWLLTYSDLITLLLAFFVVMYSMSQVDAK
                     RFGQMAKALHGILKGGENVFDYDEAKPTEDGHGLLKLGNMRMLQARVEERFKGINRLD
                     DVSSEITERGLVIHIVDRALFDEGAADLKPQAIDVLDLINDEVRAMPNHLRIEGHTDD
                     RQIATLRFPSNWELSAARATEVVRFFVNQHDFPPDRISALGYGEFRPIRPNNSIENRA
                     MNRRVDIVILTMELSLKEPTSQLYFTAANE"
     gene            complement(4141..4905)
                     /locus_tag="PK112_00615"
     CDS             complement(4141..4905)
                     /locus_tag="PK112_00615"
                     /inference="COORDINATES: protein motif:HMM:NF006583.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HPC10585.1"
                     /translation="MDLATLIGLVVAFGAVGGSFLMEGGSFDAVFLAAPILIVVGGTL
                     GATTVTTSIDTVNHIPHYLRIAFFGRSHRFSDTIEQIVKLGEKARREGILGLERDMER
                     IRNPFFRKAVRLVIDGTETSALREILETEIAYLEARHKRGITFFQKAGGYSPTMGILG
                     TVLGLVHTLGNTSDASRMAAHIAAAFIATLWGVGLANLFFLPVSDKLRMRHEEEIAHL
                     ELIMEGIMALQSGENPRHIRTRLLAFIAPIHRNHEE"
     gene            complement(5117..>5731)
                     /locus_tag="PK112_00620"
     CDS             complement(5117..>5731)
                     /locus_tag="PK112_00620"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPC10586.1"
                     /translation="NLQSKLLRVIQEREFERVGSSQTIQVDVRLVATSNRNLREYIAA
                     GRFREDLYYRLNVIPIHLAPLEERKEDVPLLVEHFIAKYNRENSRTVKGVDAQAMRLL
                     MHYHWPGNVRELENYIERAVVTGNKEILTEDEFPAELALGRAADSLAALRVPMKLEEG
                     NKYLILKTLEKFNGNKTRAAEALGISARTIRNKLAEYGEEVSNS"
BASE COUNT          875 a         1804 c         1913 g         1139 t
ORIGIN      
        1 attcgtactt gagcgctccc atcagacgtc actcatcatg gttttgatcg cgcgggcgat
       61 ctcttcgggc tgcttgcggg ccgcgtcctg catctcatcg accaggcggg ttcgccgctg
      121 ctcggggatc acgcgtggcc cttcctccgc ggcggcttcg ggggccgcgc ccggcgtcag
      181 cggccgggcg ggggtccgtt tcggcgccgc ggccatggcc ccgatcgact ggaacagccg
      241 gctcaccttg cgccgcaggt acaggaagac caggagggcg agcaggacaa ggccgacttt
      301 cttggcgatt tcgaggtaga aatcccgccg gtacatgctg tcgagagcct gctggtcctc
      361 gatcaggtcg cggcggtcga aggcgatgtt gaccatttcg atctggtcgt tgcgctggga
      421 gtcgaagccg acggcgttgc ggacgatcgc ggccagccgg tccagttcct cctgggagcg
      481 gggctggtag gacatcgccg cgccgccgtc ctcggcggcc gccggggtat agacgccgtc
      541 caccatcacc gccaccgaga ggcgtttgat cgagccgatc ccgttgatga tgtgctcgac
      601 cgtcttgttg agttcgtagt tggtgatggt cgtttcggtg gatccctgtt cctggctctc
      661 gctggcctcg gcggccttgt cggtcgaggc gttggtctgc ttggtgcgtt cttcgctgcg
      721 cacggccggc tggttggcgt cgtacgtctc cgaggtccgc tcggtctggt tgaagtcaag
      781 ttcggcggtg acccggatca cggagcgtcc cggcccgagc acgtcgtcca gcatcgactg
      841 cgccttgtgt tcgagatagg tctcggcctg tttgcgcacg tcgagctgcg aggcggtcag
      901 tccggccagc tcatcggtcg gctggcccga ggagagcagg ttgccgtcgt agtcgacaat
      961 cgagatattt tcgggccgca ggccttccac cgaggccgcg acgaggtggg agatcccgtt
     1021 gatctgctgt ttggtcaggc cgccgctgcg cagtttgagg accaccgaag cggtggcctg
     1081 tttctggtcc tgcttgaaga ggcggtcgga aggcatcacg atgtggaccc gggcggcctg
     1141 cacctccgac aactgcatga tcgtgcgcgt caactcccct tcgagggcgc ggcggaaatt
     1201 gaggttctgc aggaagtcgg tcatcccgag gttgttcttg tcgaagatcg agtagccgat
     1261 gttgccgttg cggggaaggc cctcggaggc cagcgcaatc cgcgtcttgt agacctggtc
     1321 ggagggtacc gcgatcgtcc gcccgccgtc gctcaactcg taggggatct tgttgtcgtt
     1381 caggtaggcg accacctcgc cggcctcggc ctcatcgacg tcagaataga ggcgggcgta
     1441 ggtgacggta ttgacccagc cgacgaggag gatcgtgccg acgatggtgc cggcggcgat
     1501 tcccagcagc atcatgacct ggccgggcga gagccggccg acccacgcgg cgaggttgcg
     1561 gaaaaactgt ctcacttgct ccatggcatc atcctatgtc acgtccgcgg ccggtccctg
     1621 gccgcgcacc cacgttggct gccgactaca agggcatctg tataagctgc tggtatccct
     1681 cgaccagctt gttgcggatt tcaatgagga gctcggcggc gaccccggct tcctccgctt
     1741 tcaccatcac ctggtgcagt tcgaccggct cgccttcgag aaacgcctgc tggatctgcg
     1801 ccgattcgta ctgcagcgag ttgacgccgc tgacaaagtc ggagagcatc tcaccgaaac
     1861 cgtcctcggc cttcatctcc ggcaacggca cggtctttcc agtcggctcg agccgcgcca
     1921 cgagtcccgg caccaaccgg gtgaggccat tgacaggtgc cgtcatgtta caccttcacg
     1981 tcgatcagtt taccgagcac ccgggactcg gcggcctcgc cgcccagggc cgctccgaag
     2041 cggccggagt cgccgaagcg ctggaagagc agctccaggg ccgcgcgctc ggcctcggtg
     2101 aggaaatcgg cgtaggatcc ctcgggcgct ttgaccgcga ggtcggatcc ggacgcggcc
     2161 gcctgcggcg cgatggtcag ctcgcgtgcg gcgtcggccg gccgggcctg ctcggccggg
     2221 cgctgcggct gccgctgcac gagctgctgg tacgactgaa ttgctgtcgg gttaatcttc
     2281 atggctgcct gtcactttcc tatatgtcca gtgcatcttt ggccatctgc ttggcggcgg
     2341 cgatcgtcac cgtgttggcc tcgtaggcgc gcgccgccgc catcatgtcg accatctcgt
     2401 tgaccacctc aatgtccggc atcttcacgt acccctgctc gtcggcgtcg ggatgctcgg
     2461 gatcgtggat gagccggtag ctcgattgcg gatcccggat ttccttcatg tcgacgtcgg
     2521 ggacctccac ctcagattgc acacacacgc cgcgggcggg gaaatgccgg gtgttggtgc
     2581 gctcgaggga gacgccggcg cgccgcatgg cctgccggaa cgacaccccc gtctcgtctt
     2641 cccgcacctg cacgcgcttg cgccggtaag ggccgccctc cggggtctgc gtggtctcgg
     2701 cgttggcgat gttttcggcc gtggcgttca tttttgcccg ctggaccgac agtccgcgcg
     2761 acgcaatctc aatggctccg agtatgcccg ccatacctta ctctcctatc tccccgctat
     2821 ggcccgccgc agcccctcaa atttcttctg caggagccga gcggccaccg taaactgaag
     2881 ttcgttctgg gccatctgcg agacctcccg gtcgatgtcg acggcgttga gctcatccgg
     2941 tttgggtctg gtctcctcga tatccggttc gcggtcgcgg tgcgcgccga gaggcaggtg
     3001 cgcggggtgg gtcaactcgc cggccacccg gccggtctcg ccggtgaggc gcgtgtactc
     3061 ggcttgaaag tcgatgtccc gggcgccgta cccgggcgtc gacacgttgg ccacgttgct
     3121 gctgatcagc ttgtgccgga acgaggagac atccaggtac gcctcgaatc tgtggacgcc
     3181 ggtcgacttg aagagaaagt ccgctatttt catacggtca cacacggcct catggttttt
     3241 ctcgcgctgc gtctatgcaa cgtgcgtgcc gaatcgcggt cggacgccgc aggcggttgc
     3301 ggcccagcgg gttggactga cgcgcgcggt cccacagcgc ggggcgagcc cgaatgtcag
     3361 ggaaagtatt tccggaggag ccgggaaaag actactcgtt ggcggccgtg aaatacaact
     3421 gcgaggtcgg ttctttgagc gagagttcca tggtgaggat cacgatgtcg acgcgccgat
     3481 tcatggcccg gttctcgatc gagttgttgg ggcggatcgg ccggaactcc ccgtagccga
     3541 gcgcggaaat gcggtcgggc gggaaatcgt gttggttcac gaaaaagcgc acgacctcgg
     3601 tcgcgcgggc cgccgagagc tcccagttgg agggaaaccg cagcgtggcg atctggcggt
     3661 cgtcggtgtg cccctcgatc cggaggtggt tgggcatagc gcggacttcg tcgttgatga
     3721 gatcgaggac gtcgatcgcc tgcggcttca ggtcggccgc cccctcgtcg aacagcgccc
     3781 ggtcgacaat gtgaatcacc agcccgcgct cggtgatctc cgagctgacg tcgtccagcc
     3841 ggttgattcc tttgaagcgt tcctccacgc gggcctgcag catgcgcatg ttgccgagtt
     3901 tcagcaggcc atggccgtcc tcggtcggtt tcgcctcgtc gtagtcgaag acgttctccc
     3961 ctcctttaag aatgccgtgg agagccttgg ccatctgccc gaagcgcttg gcgtcgacct
     4021 gcgacatgga gtacatgacg acgaagaacg ccagaagcag cgtgatgagg tcgctgtagg
     4081 tgagcagcca gcgatcgagg ttctccttgt cttcgacccg gcggcgaaag cggcgcatgg
     4141 ctactcctcg tggttgcggt ggatcggggc gatgaaagcg agcaggcggg tgcggatgtg
     4201 ccgcgggttc tcaccggact ggagggccat gatcccctcc atgatcagtt cgaggtgggc
     4261 gatctcctcc tcgtggcgca tgcgcagttt gtccgagacc gggagaaaga acaggttggc
     4321 cagtccgact ccccagaggg tggcgatgaa cgcggccgcg atatgggccg ccatgcgcga
     4381 ggcgtccgag gtgttcccca gcgtgtgcac caggccgagg acggtcccga ggatccccat
     4441 ggtcggcgag tagccgcccg ctttctggaa gaaagtgatg ccgcgcttgt gccgcgcctc
     4501 gaggtaggcg atctcggttt cgaggatttc gcggagcgcg ctcgtttccg tcccgtcgat
     4561 gaccaggcgc acggctttcc ggaaaaacgg gttccggatg cgctccatgt cgcgctcgag
     4621 gccgagaatg ccttcccgcc gggccttctc ccccagtttc acgatctgct cgatcgtgtc
     4681 gctgaaccgg tgcgaccggc cgaagaaggc gatgcgcagg tagtggggaa tgtggttgac
     4741 ggtgtcgatc gaggtggtca ccgtggtcgc cccgagcgtg ccgccgacca cgatcaggat
     4801 gggcgcggcg aggaagacgg cgtcgaagga tcctccctcc atgaggaacg acccgccgac
     4861 cgcgccgaac gccaccacca aacctatcag tgtcgccaga tccatgggta tgtcaactcg
     4921 gttacgaccg caccggggtc accgtcccgg cccgcggccg tgacgggtgg ggcggggcgc
     4981 cggcgttttc ggccgtccga cgtctgtgcg ccgggcgtcc cgcacatcct accccaatac
     5041 tatcggcagg ggggatgatt agtggagagc gggcggggcg gcaactgccc gccccgtacc
     5101 cgcttgcaga aactgctcag gaattggaca cttcttcgcc gtactcggcc agcttgttgc
     5161 ggatggtccg ggccgagatc cccagcgcct cggcggcccg ggtcttgttg ccgttgaact
     5221 tctcgagcgt cttgaggatc aggtacttgt tcccctcctc cagtttcatc ggcacccgca
     5281 gagccgccag gctgtcggcc gcccgaccga gagccagctc ggcggggaac tcgtcctcgg
     5341 tgaggatttc cttgttcccg gtcacgaccg cccgctcgat atagttttcc agctcccgca
     5401 cgttgcccgg ccaatggtag tgcatcagca gccgcatcgc ctgagcgtcg acccctttca
     5461 cggtccggct gttctcgcgg ttgtacttgg cgatgaagtg ctcgaccagt agcgggacgt
     5521 cttccttgcg ctcctccagc ggggccaggt ggatcgggat gacattcagg cggtagtaga
     5581 gatcctcgcg gaaccgtccg gcggcgatgt actcgcggag attccggttc gaggtggcga
     5641 ccagccggac atcgacctgg atggtctggc tcgatcccac ccgttcgaac tcccgctcct
     5701 ggatcacgcg gagcagcttg gactgcaagt t
//
LOCUS       DAOOKS010000019         5648 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_17167_length_5648_cov_3.802074, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000019 DAOOKS010000000
VERSION     DAOOKS010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5648
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17167_length_5648_cov_3.802074"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /locus_tag="PK112_00625"
     CDS             complement(<1..851)
                     /locus_tag="PK112_00625"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPC10587.1"
                     /translation="MTRIRTGVVGVGALGRHHLRWFSQIPESHLVGFHDIDPEKRERY
                     AAEYGVAAFATVEELAEAVDAASVAVPTTAHRAAAGALLKRGVHCLIEKPVTSTLDEA
                     HELAELAAASGARVAVGHIERFNPAVQAVLRIGVRPAFIEAHRLAAFDPRGTDVAVVR
                     DLMIHDIDLVLTLTGARVVDIQASAVAVVSDQPDIANARLTLDSGAVANLTASRISLN
                     QMRKLRIFQRSGYFSLDLAKKQADLYRLAAPGEEIEGLRVPLGKSGRELIYASHGDTG
                     QDQLQMEL"
     gene            complement(1014..1481)
                     /locus_tag="PK112_00630"
     CDS             complement(1014..1481)
                     /locus_tag="PK112_00630"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPC10588.1"
                     /translation="MSQTAVVTFRAAKETEYAGFLDLIHQHAADYLEPSLTLLGLDKT
                     EFAHLFRTRGQVVSILADRAVAGFYWIEERDGTLHIHGLVIKPEFQKRGLAAAALAHL
                     ETGASPDVARFELGVHRANGRARKIYERAGYRVTRALPDFDFSIMHKDRPAQS"
     gene            complement(1517..2290)
                     /gene="lpxA"
                     /locus_tag="PK112_00635"
     CDS             complement(1517..2290)
                     /gene="lpxA"
                     /locus_tag="PK112_00635"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HPC10589.1"
                     /translation="MSGIHPTAIISPAAELAEDVSVGPYTVIEGDVRIGPGTAIASSC
                     LIASGARIGERCVIAHGAVIATQPQDLKFGGEKTFVEIGDGTLIREYATVNRGTAAHG
                     TTTVGKGCMLMAYSHVAHDCILGDHVILANAVNLAGHVEIDDYVIIGGIVPVHQFVKI
                     GAHAMIGGGFRVPQDVCPYSLCGGYPLRVMGINAVGLRRRGFTRDAVAAIEQAFKILF
                     FSKLNTSQALERIRAEMEITPEVGRILEFADRSTRGLTK"
     gene            complement(2287..3678)
                     /locus_tag="PK112_00640"
     CDS             complement(2287..3678)
                     /locus_tag="PK112_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010933326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HPC10590.1"
                     /translation="MYEKQRTIRRPISLSGIGLHTGNLCTMTFHPAPPDHGIRFVRVD
                     LPDRPSVIADIDHVVDISRGTTLQDGEAKVHTVEHVLAAFAGLQLDNMLVELDNNEPP
                     IGDGSAKPYVDKILEAGIESQDADKVYLEIDTPMAYNERERGVDLVVAPSNDLRITFL
                     IDYKNPALGTQYTTLQDLEQEFVDEFAPARTFCFLSEVESLKAAGLIKGGSLDNAVVI
                     YDSDRGQVEVDRIRRALDLKEEAFVGKTGIINDIPLRFYNEPVRHKALDLLGDLFLIG
                     VPFKGHVLAARSGHKANVALARKMRDLYKKKKIASRYAGRASAALFDIDAIQRIMPHR
                     YPFLLIDRILDLEPDTRVSALKNVTINEPFFQGHFPGHPIMPGVLILEAMAQAGGVLL
                     LNAIPQPETKVVYFMSIDNAKFRKPVVPGDQLRFELQMISFRRMTCKMAGEAYVDGEL
                     VASAEFLAMVRDR"
     gene            complement(3733..4101)
                     /locus_tag="PK112_00645"
     CDS             complement(3733..4101)
                     /locus_tag="PK112_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10591.1"
                     /translation="MFSNNEERIRYFASTMNAGRILVNTPASQGALGGTYNALQPSLT
                     LGCGTGGKNITTDNISARHLLNIQRIARRRVSPCIGPEMMAAYLDETVDALALERRCA
                     EMEAGAARPGDPAGEPPKKT"
     gene            complement(4053..5222)
                     /locus_tag="PK112_00650"
     CDS             complement(4053..5222)
                     /locus_tag="PK112_00650"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPC10592.1"
                     /translation="MGEPAGTRLDVDGIMNRAAAAAEAFRRCDQEHTDRIVTAVYRAG
                     FDQRIPLAKMAAEETGLGVWQDKVIKNVIATRFVYENIKTLKTVGVIAENEEAGIVEI
                     AQPIGPIFAVTPITNPTSTVLFKILIALKARNPIVIRPHGAARKCSIEAARICYEAAL
                     AAGAPEHCVQWVRRSTEEETLALTRHRKTALILATGSEALVRAAYSSGNPAIGVGPGN
                     VPVFIGKTADVPFAVDQIVMSKTLDHGTVCASEQALVVRACHAAETRRLLIERGAYFL
                     TPEEIRRLETVAFNPATRVMRVEVIGQPAEKIARMAGIEAPAGTRLLVAELEDVGPHT
                     PLSLEILLPSWPSTWPRISTRRSPSAAKSTTTGDWATPSACSQTTRSGSATSPPP"
     gene            complement(5330..>5648)
                     /locus_tag="PK112_00655"
     CDS             complement(5330..>5648)
                     /locus_tag="PK112_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10593.1"
                     /translation="LLEPGGVQQALTAELAEPVDIVCRAIMSKDLDKASENASGSRLM
                     AGVDYELFFWGGEWKSLGTATAAEEPLVFLAPSGGLYRLAAAGSDGDERIFTWREGAQ
                     TWW"
BASE COUNT          871 a         1856 c         1908 g         1013 t
ORIGIN      
        1 agctccatct ggagctggtc ctggccggtg tcgccgtgcg aggcatagat gagttctcgc
       61 cccgatttcc cgagcggtac cctgagtccc tcgatttcct ctcccggcgc cgcgaggcgg
      121 tagaggtcgg cctgcttttt cgcaaggtcg agcgagaaat agccggagcg ctggaagatc
      181 ctgagcttgc gcatctggtt gagcgaaatg cgggaggccg tgaggttggc caccgcgccg
      241 ctgtccaggg ttagccgggc gttggcgatg tcgggctgat cggacacgac cgcgacagcc
      301 gaggcctgga tgtcgacgac ccgggcgccg gtgagcgtca ggacgaggtc gatgtcgtgt
      361 atcatgaggt cgcgcacgac ggcgacgtcg gtgccgcggg gatcgaaggc ggcgaggcgg
      421 tgggcttcaa tgaaggcggg ccggacgccg atgcggagca ccgcctgcac ggcggggttg
      481 aaccgttcga tgtgtccgac cgccacccgg gcgccgctgg ccgcggccag ttccgccagc
      541 tcgtgcgcct cgtccagcgt cgacgtcacc ggtttctcga tcaggcagtg caccccgcgt
      601 ttcaaaagcg cgccggcggc cgcccggtgc gcggtggtcg gcacggcgac gctcgccgca
      661 tcaaccgctt ccgccagctc ctcgaccgtc gcgaaggccg ccaccccgta ttcggcggcg
      721 tagcgctccc gcttttccgg gtcgatgtcg tggaagccga ccaggtgcga ctcggggatc
      781 tgcgagaacc agcggaggtg gtgtcgcccg agcgcgccga caccgaccac gcccgtccga
      841 atccgtgtca tagctgctgc tttccctgtc cgtgacggtt ccgcgccgcc gcgcgcccgc
      901 ccgggccggg cggcggtatg ccgtcgtttg cgatcgacgg gaaagtaata caaacggcgg
      961 cgccgcaaaa ggacaatgtt gttccccgcg gcgctgtcgg ccgcggcggc ccttcagctc
     1021 tgcgcgggac ggtccttgtg catgatggag aaatcgaagt cgggaagcgc ccgcgtcacc
     1081 cggtagccgg cccgctcata aatcttgcgg gcgcggccgt tggcgcggtg cacgcccagc
     1141 tcgaaccggg ccacgtcggg cgatgcaccc gtttcgaggt gggccagagc ggccgccgcc
     1201 aggccccgct tctgaaactc cggcttgatc accagtccat ggatgtggag ggtcccatcg
     1261 cgctcctcaa tccagtagaa cccggccacg gcgcggtcgg cgagaatact caccacctgt
     1321 ccccgcgtgc ggaacagatg ggcgaactcg gtcttgtcca ggccgagcag cgtgagcgac
     1381 ggttccaggt aatcggcggc gtgctggtgg atcagatcca gaaaccctgc gtactcggtt
     1441 tccttcgccg cgcggaaggt gacaactgcg gtttgagaca tgggccactc tcccctctga
     1501 cgggttggcg tttgctctat ttcgtcaggc cgcgggtcga gcggtccgcg aactcgagga
     1561 tgcgcccgac ctccggcgtg atctccattt ctgcccggat gcgttccagc gcctgggagg
     1621 tgttgagttt gctgaagaag aggattttga aggcctgttc gatggcggcg acggcatcgc
     1681 gggtgaagcc ccgccggcgc aggccgacgg cgttgatgcc catgaccctg agcgggtacc
     1741 cgccgcacag gctgtacggg cagacgtcct ggggcacgcg gaatcccccc ccgatcatcg
     1801 cgtgcgcccc gattttcacg aactggtgga cgggcacgat gccgccgatg atgacgtagt
     1861 cgtcgatctc cacgtgtccg gcgaggttca ccgcgttggc caggatcacg tggtcgccga
     1921 ggatgcagtc gtgggcgacg tgggagtagg ccatcagcat gcaccccttc cccaccgtcg
     1981 tcgttccgtg ggccgcggtg ccccggttca cggtcgcgta ctcgcggatg agggtgccgt
     2041 cgccgatctc cacgaacgtt ttctcgccgc cgaatttgag gtcctggggc tgggtggcga
     2101 tcaccgcgcc gtgggcgatc acgcagcgct cgccgatgcg ggcgcccgag gcgatcaggc
     2161 aggaggaggc gatcgccgtc cccggcccga tccgcacatc accctcgatg accgtgtacg
     2221 gtccgaccga aacgtcctcg gccagctccg cggccggcga tatgatcgcg gtcgggtgga
     2281 tgccgctcat cggtccctca ccatggcgag gaactcggcg ctggccacca gctcgccgtc
     2341 gacgtaggcc tcgcccgcca tcttgcaggt catgcgccgg aacgagatca tctggagctc
     2401 gaaacggagc tggtcgccgg gcacaaccgg cttgcggaat ttcgcgttgt cgatcgacat
     2461 gaagtacacc actttggttt ccggctgcgg gatggcgttg agcaggagca cgccgcccgc
     2521 ctgcgccatt gcctcgagga tcagcacgcc cggcatgatg gggtggccgg ggaaatgccc
     2581 ctggaaaaac ggctcgttga tcgtcacgtt cttcagcgcg ctcacccgcg tgtccggctc
     2641 gagatcgagg atgcggtcga tcaggagaaa ggggtagcgg tgcggcatga tccgctggat
     2701 ggcgtcgatg tcaaagagcg cggccgaggc ccgcccggcg taccggctcg caatcttctt
     2761 cttcttgtac aggtcgcgca tcttgcgcgc gagggccacg ttggccttgt gccccgagcg
     2821 ggcggccagc acgtgcccct tgaacggcac gccgatgagg aacaggtcgc cgagtagatc
     2881 gagcgccttg tgccgcaccg gttcgttgta gaaacgcagg ggaatgtcgt tgatgatccc
     2941 ggtctttccg acgaatgcct cctccttcaa atcgagcgcc cggcggatcc ggtccacctc
     3001 gacctggccg cggtcggagt cgtagatcac gaccgcgttg tccaggctcc cccccttgat
     3061 cagcccggcg gccttgagcg attccacctc ggagagaaaa cagaacgtcc gcgccggtgc
     3121 gaattcatcg acaaactcct gctcgagatc ctgcagcgtc gtgtactgcg tccccagagc
     3181 cgggttcttg tagtcgatga ggaaggtgat gcgcaggtcg ttcgagggcg ccaccaccag
     3241 gtcgaccccg cgctcccgct cgttgtacgc catcggcgtg tcgatctcca ggtacacctt
     3301 gtcggcatcc tgtgactcga tccccgcctc gaggatcttg tcgacatacg gtttcgcgga
     3361 accgtcgccg atgggcggct cgttgttgtc cagctcgaca agcatgttgt ccagttgcag
     3421 gccggcgaaa gcagcgagca cgtgctcgac cgtgtggact ttcgcctcgc cgtcctgaag
     3481 cgtcgtgccg cgcgagatat ccacgacgtg gtcgatatcg gcgatgaccg aggggcggtc
     3541 ggggagatcg acgcggacga agcggatgcc gtggtcgggg ggggcgggat ggaacgtcat
     3601 cgtgcacagg ttgccggtat gcaatccgat gccggacaaa gaaataggcc gcctgatggt
     3661 ccgttgcttc tcgtacatac ttccgttact cgatcgtagt ttggtcgatg atacaagaaa
     3721 ggaggaaccg gctcaagtct tttttggcgg ttctccggcg gggtcgccgg gccgcgcggc
     3781 gcccgcctcc atttccgcgc accgccgctc cagcgcgagg gcatcgaccg tctcgtccag
     3841 gtacgccgcc atcatctctg ggccgatgca cgggctgacg cggcggcgcg cgatccgctg
     3901 gatattgagg aggtggcggg ccgagatgtt gtcggtggtg atgttctttc cgccggtccc
     3961 gcagccgagg gtgagcgacg gctggagggc gttgtaggtt ccgcccagcg ccccctgcga
     4021 ggccggcgtg ttgaccagga tccgcccggc gttcatggtg gaggcgaagt agcggatccg
     4081 ctcctcgttg tttgagaaca tgctgatggt gtggcccagt cccccgttgt ggttgatttt
     4141 gcggcagagg gcgatcgcct ggtcgatatc ctcggccacg tagaaggcca ggacgggagg
     4201 aggatttcca gcgagagagg cgtgtggggg ccgacatcct caagctcggc gacgagcagc
     4261 ctcgtcccgg cgggcgcctc gatccccgcc atccgcgcga tcttctcggc cggctggccg
     4321 atcacctcga cccgcatgac gcgggtggcc gggttgaagg ccaccgtttc aagccggcgg
     4381 atttcctccg gggtgaggaa gtacgccccc ctctcgatga gcagccgccg cgtctcggcg
     4441 gcgtggcagg ctcgcaccac gagcgcctgc tcgctcgcgc acaccgtgcc gtggtcgaga
     4501 gttttcgaca tgacgatctg gtcgacggcg aacggaacgt cggccgtctt gccgataaac
     4561 accggcacgt ttcccggacc gacgccgatg gcggggttgc cggagctgta ggcggcccgg
     4621 acaagcgcct ccgaaccggt cgcgagaatc agcgccgtct tgcggtgccg agtgagggcg
     4681 agcgtttcct cttcggtcga ccggcgcacc cactggacgc agtgctcggg ggcccccgcc
     4741 gcgagcgccg cctcgtagca gatgcgcgcc gcctcgatcg agcacttccg cgccgccccg
     4801 tggggacgga tcacgatcgg gttgcgcgct ttcaacgcaa tgaggatctt gaacagcacg
     4861 gtcgaggtcg ggttggtgat cggggtgacg gcgaagatcg gtccgatcgg ctgggcgatc
     4921 tcgacgatcc ccgcttcctc gttctcggca atcaccccca ccgtcttcag ggtcttgatg
     4981 ttctcgtaga caaaccgggt ggcgatcacg ttcttgatca ccttgtcctg ccacactccg
     5041 aggccggttt cctcggccgc cattttcgcc agggggatgc gctggtcgaa cccggcccgg
     5101 tagaccgcgg tcacgatgcg gtcggtatgc tcctggtcgc agcggcggaa ggcctcggcc
     5161 gcggccgccg cccgattcat gattccgtca acgtcaagtc gcgtgcctgc cggctcgccc
     5221 atagtctgct ccctcgctgc ccggtaagtg tgaacacctg tgaaggctgc cggaaatccc
     5281 gcccggcgag agttccggac cggtcacgat cgcccccccg ccggcccgct caccaccacg
     5341 tctgcgctcc ctcgcgccac gtgaagatcc gctcgtcccc gtcggagccc gccgccgcga
     5401 gccggtagag cccgcccgag ggggcgagga acacaagggg ttcttcggcc gccgtcgccg
     5461 tcccaagcga cttccattcc ccgccccaga agaacagctc atagtccacc ccggccatca
     5521 gccgggagcc ggaggcgttt tccgacgcct tgtcgaggtc tttgctcatg atcgcccgac
     5581 agacgatgtc gaccggctcg gcgagctccg ccgtcagcgc ctgctgaacc ccgcccggct
     5641 cgaggagg
//
LOCUS       DAOOKS010000020         5638 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_17203_length_5638_cov_3.371664, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000020 DAOOKS010000000
VERSION     DAOOKS010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5638
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17203_length_5638_cov_3.371664"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1218
                     /locus_tag="PK112_00660"
     CDS             <1..1218
                     /locus_tag="PK112_00660"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HPC10594.1"
                     /translation="EVAEKQTGQVSAGAGYNSQDKVVGTFGMGIPNFRGLGQNLSFNV
                     SLGKSYNSFSVSFTEPWMFGYPTSFGASAFSLNRRWYGDYTEGRAGGSLRLGRRLRWP
                     DNYFRVYASYALERTRYYDFDATFAASNSYQSRYYYNNPETISPADSLMAARTYGPYP
                     GSILEYEDWQTASRVSLTLLRDSRNLPEFATKGSILSYTLELTGGLLGGYWEYTKHDF
                     EAAKFIPLMWGWALAAKAQYGVVTAPAGDARIMISDRFTPGGTAYDGIVRGYDDGSLT
                     PDSAITTDTIFYYSNENAVIGVDPPDGRYFPLDTTRVRGKYMFVANVEVQIPIAARQL
                     YGLLFFDAGNSWLYRRDIDLLNDLYKSVGFGFRIIVPGIGTLGFDFGYPLDDVEGEPR
                     SWKPHFQIGTTFR"
     gene            1243..1773
                     /locus_tag="PK112_00665"
     CDS             1243..1773
                     /locus_tag="PK112_00665"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HPC10595.1"
                     /translation="MRVTKAASVLSAVLALTVILSGAAAAQTLKIGFVRDDRIYAEYT
                     EWQRAQETWETDRKAWDDEASAKEQELQELTADYEKQRLILSDEKKREREAAIRAKRE
                     GLDAYTKQIYGPGGTAEQREAQLIEPLLTKIRQAMEAVATENSYDVIFTLQGIGYIKE
                     EYDVTDKVLAALNEME"
     gene            1833..2849
                     /gene="lpxD"
                     /locus_tag="PK112_00670"
     CDS             1833..2849
                     /gene="lpxD"
                     /locus_tag="PK112_00670"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HPC10596.1"
                     /translation="MAFTVAYLAERVGGTVAGDGSVEIGAAAPIETAGPGDISFIANP
                     RYARFLATTRAGAVVVSPQTECPRLPLIRHAQPYLAFACILDLLYPDERLVAPGIDPR
                     AVVAAGAVVDPTAGVGALCHIREGVRIGAGTELVSSVFVGRDVVVGDNCRIYPGVCLM
                     DGTRLGNNVIIHSSTVIGSDGFGFAPSPTGLKKIKQVGWVEIDDDVEIGSNCSVDRGA
                     LGPTRIGRGTKIDNLVQIAHNVEIGRHAIIVSQVGISGSTRVGNGVVLAGQVGLVGHI
                     EVGDGVQVGAQSGVARSVPAGKKVFGSPARDLAETGRIEAALVRLPELLKRVRRLEDK
                     LGGE"
     gene            complement(2853..3431)
                     /locus_tag="PK112_00675"
     CDS             complement(2853..3431)
                     /locus_tag="PK112_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10597.1"
                     /translation="MSYASRFIPRTDTPEGQQVATRAMLLIGSAVTLSALIIIAFNWT
                     LSRSTLEQIGSGVELAALTLVPYMIAAAVAAITALCVLLMVPAAQLRQPATVIRDRLQ
                     ALANGDLASRIQGRKPAPHVQELVYELNLATSNLSHQIAQWKVINRQQWELLEAMREI
                     TVVDGHRQLVRIIEQMQANWEKIAEIEERLKT"
     gene            complement(3604..4032)
                     /gene="ssb"
                     /locus_tag="PK112_00680"
     CDS             complement(3604..4032)
                     /gene="ssb"
                     /locus_tag="PK112_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019024082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HPC10598.1"
                     /translation="MGVNKAILIGRLGKDPELKYTASGRPVTTFTLATTERWTNQEGQ
                     KQEQTTWHNIVAWGRPAEIMNEYLAKGREVYIEGRIVNRSYDDKDGNKRHISEIVVQN
                     FQFIGSGREGGGQGGGERAPAAEDAAAPEGAAPAEDDLPF"
     gene            4279..5094
                     /locus_tag="PK112_00685"
     CDS             4279..5094
                     /locus_tag="PK112_00685"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF010587.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HPC10599.1"
                     /translation="MEARPEIDLDRAVTTITRFLTGKLLQSGLDGYVVGVSGGLDSAV
                     AAALAVAAVGPDKVLGLLLPYRTSSAESLHDARLMVERLGIPHRVIDISPMIDAYYPR
                     ITDELKRRAGNKMARERMAILFDVALETSRLVLGTGNRSEIALGYTTWHGDSACSVNP
                     IGELYKTEVRLLARKLAVPEPIIAKPPTADLWKDQTDEGEIGVTYPQIDRLLARIVDQ
                     GVRSMSQLAAEGFPPTEISRVVSLLNRNAFKRALPEVAPLQRKPIPANVQLSE"
     gene            5105..>5638
                     /gene="rsmI"
                     /locus_tag="PK112_00690"
     CDS             5105..>5638
                     /gene="rsmI"
                     /locus_tag="PK112_00690"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HPC10600.1"
                     /translation="MPESPGLLHLVPTPSGNMADLTRRAEEMLAAADLVACEDTRHSG
                     RLLKALGLNKRLISYHDFNERTRAEQLIDLIRQGQTVAVITDAGSPGISDPAYRIVRT
                     AIDNGITIIPLPGPTSIIPALTASGLPTDRFFFEGFLPHKSGARRTRLARLTEFDHTL
                     VFFESPHRVVRSLEDIRE"
BASE COUNT          976 a         1859 c         1795 g         1008 t
ORIGIN      
        1 gaagtggccg aaaagcagac cgggcaggtg tcggccggcg ccgggtacaa cagccaggac
       61 aaggtggtcg gcacgttcgg catgggcatc ccgaatttcc gggggctcgg ccagaacctg
      121 tctttcaacg tcagtctcgg caaatcgtac aactccttct cggtctcgtt caccgagccg
      181 tggatgttcg gctatccgac ctcgttcgga gccagcgcct tctctctcaa ccgccgctgg
      241 tacggcgact acaccgaggg gcgggcgggc ggctcgctcc gcctggggcg acggctgcgc
      301 tggccggaca actatttccg cgtctacgcc tcctatgccc tggagcggac gcgctactac
      361 gatttcgacg cgacctttgc agcctccaat tcctaccaat cgcggtacta ctacaacaac
      421 ccggagacca tctcgccggc ggattccctc atggccgccc ggacctatgg gccgtacccg
      481 ggttccattc tcgagtacga agactggcag acggcatcgc gggtgtcgct gacgcttctg
      541 cgcgactcgc gcaaccttcc ggaattcgcc accaagggtt ccatcctctc gtacacgctc
      601 gagttgaccg gggggctcct cggcggctac tgggagtaca cgaagcacga cttcgaggcg
      661 gccaagttca tcccgctcat gtggggctgg gcgctcgccg ccaaggcgca gtacggggtt
      721 gtgacggccc cggctggcga cgcccgcatc atgatctccg accgcttcac gcccggcggc
      781 accgcctacg acggcatcgt ccgcggctac gacgacggca gcctgacgcc cgactcggcc
      841 atcaccaccg acacgatctt ctactacagc aatgaaaacg cggtcatcgg ggtcgatccg
      901 cccgacggca ggtattttcc gctggacacc acccgggtcc gcggcaagta catgttcgtg
      961 gccaacgtcg aagtgcagat acccatcgcc gcccggcagc tctacggcct gctgtttttc
     1021 gacgccggca actcctggct ctaccgccgc gacatcgatc tgctcaatga cctgtataag
     1081 agcgtgggat tcggcttccg catcatcgtc ccgggcatcg gcacgttagg gtttgacttc
     1141 gggtacccgc ttgacgatgt tgaaggcgaa ccgagaagct ggaagcccca tttccagatc
     1201 ggaacaacat tcagataaga atacgagcaa gtgaggattc gcatgcgagt aacaaaagct
     1261 gcatcagttc tgtccgccgt cctggcgctc acagtcatcc tgtccggggc ggccgccgcc
     1321 cagaccctga agatcggttt cgtgcgcgac gaccgcatct acgccgaata caccgagtgg
     1381 cagcgcgccc aggaaacctg ggagaccgac cggaaagcgt gggacgacga agccagcgcc
     1441 aaagagcagg aacttcagga gctcaccgcc gactacgaga aacagcgcct gatcctctcc
     1501 gacgagaaga agcgggagcg cgaggcggct attcgggcca agcgcgaggg tctcgacgcc
     1561 tacaccaagc agatctatgg tccgggcggc accgcggaac agcgggaggc ccagctgatc
     1621 gagccgctgt tgaccaagat ccgccaggcc atggaggcgg tggccaccga gaacagctac
     1681 gacgtcatat tcaccctcca ggggatcggc tacatcaagg aggagtacga cgtcacggac
     1741 aaggtgctgg cggccctgaa cgaaatggag taaagctccg cgccgccgcc gatccccggt
     1801 cggaggcggg cggccgcggg gcgcgaggac tcatggcctt caccgttgca tatctggccg
     1861 agcgcgtcgg agggaccgtt gccggcgacg gcagcgtgga gatcggggca gccgcgccga
     1921 tcgagacggc cggtcccggc gacatcagct tcatcgccaa cccgcgctac gcgcgctttc
     1981 tcgccacgac ccgcgccggc gccgtcgtcg tgagcccgca gaccgagtgc ccccggctcc
     2041 ccctcatccg ccacgcgcag ccctaccttg cctttgcctg catactcgac ctgctctacc
     2101 ccgatgaacg cctggtcgct cccggcatcg acccgcgcgc ggtggtggcc gcgggcgcgg
     2161 tcgtggaccc gacggccggg gtcggcgcgc tctgccacat tcgcgagggg gtgcgcatcg
     2221 gcgccgggac cgaactcgtg tcctcggttt ttgtcgggcg cgacgtcgtg gtcggggaca
     2281 actgccgcat ctacccgggg gtttgcctca tggacggcac gcgcctcggc aacaacgtca
     2341 tcatccattc ttcgacggtg atcgggtccg acggtttcgg gtttgcgccg tcaccgaccg
     2401 gattgaagaa gatcaaacag gtggggtggg tggagatcga tgacgatgtg gagatcggat
     2461 cgaactgctc ggttgaccgc ggggcgctcg gacccacccg gatcggccgg gggaccaaga
     2521 tcgacaacct cgtgcagatc gcccacaacg tcgagatcgg tcggcacgcg atcatcgtgt
     2581 cccaggtggg catctcgggc tccacacggg tcggcaacgg cgtggtgctg gccggccagg
     2641 tcggcctggt ggggcacatt gaagtcggcg acggcgttca ggtgggggcc cagtccgggg
     2701 tcgcccgctc ggttcccgcg ggcaagaaag tcttcggttc gccggcccgc gatctggcgg
     2761 agacgggccg catcgaagcg gccctcgtgc ggctgccgga actgctcaaa cgggtcaggc
     2821 ggctcgaaga caagctcggc ggcgagtagg tctcacgtct tcaggcgctc ctcaatctcc
     2881 gcgatcttct cccagttcgc ctgcatctgc tcaataatgc ggaccagttg ccggtgtcca
     2941 tccacaacgg taatctcccg catcgcctcg aggagttccc actgctggcg gttgatcact
     3001 ttccactggg cgatttggtg gctcagattc gaggtggcga ggttcagttc atacaccagt
     3061 tcctgcacgt gtggcgccgg cttgcgcccc tggattctgg aggccaggtc gccgttggcc
     3121 agcgcctgca ggcggtcgcg tatgacggtg gccggctggc gcaactgggc cgccggcacc
     3181 atcagcagga cgcacagtgc cgtgatcgcc gcgaccgccg ccgcgatcat gtacgggacc
     3241 agcgtgagag ccgccaactc cacgccgctg ccgatctgct caagcgtaga gcgggagagg
     3301 gtccagttga aggcgatgat gatcagcgcc gagagggtga cggcggaccc aatgaggagc
     3361 atcgcccggg tggccacctg ctggccctcc ggggtatcgg tacgggggat gaagcgcgag
     3421 gcatacgaca tagtctgtcc ctcctatgat cgttccgagg taaccttgta gtcggctcat
     3481 cgacggtggg gcaggctggg cttagcccaa tctcggcgca ttctccggaa ctcgcctgaa
     3541 cagtaaaagg ccctgtcatc caatggatta cagggccccg tgaaaccgtt ggtcagggtt
     3601 taatcagaac ggaaggtcgt cctcggcggg tgcggctccc tcgggggccg cggcgtcttc
     3661 cgcggccggg gcacgctcgc cgcccccctg gccgccaccc tcccggccgc tgccgataaa
     3721 ctggaagttc tgtacgacga tttccgagat gtgccgcttg ttgccgtcct tgtcatcgta
     3781 ggaccggttg acaatccgcc cttcgatata gacctcgcgc cccttggcga ggtactcgtt
     3841 cataatctcg gcggggcgtc cccaggccac gatgttgtgc caggtggtct gctcctgttt
     3901 ctggccctcc tggttggtcc accgctcggt ggtggccagg gtgaaagtcg tgaccggccg
     3961 accgctggcc gtgtacttga gctccgggtc tttgcccagg cggccgatga ggattgcttt
     4021 gttgactccc ataatcattt acctctagag gttaggcgca ctcgttctcc acctgctgcg
     4081 gcggccgctc cggaaccacg gcctgtgcac gagctggggc gcgccgcggt cgagaggtcc
     4141 gcgggctcac gccggagcat ctccgcacgg aacgaacggg acctgccgtg cgttcatggg
     4201 tacaatattt agcttgtctg cacaagcgca atcacatatt tagtggcgat ttcgaaagaa
     4261 tctattgtgt gctcagccat ggaagctcgc ccggaaatcg acctcgaccg cgccgtgacc
     4321 acgatcacgc ggtttctcac cggcaagctg ctgcagtcgg gccttgacgg ctatgtcgtc
     4381 ggcgtatccg gggggctgga ctccgccgtg gcggctgctc tggcggttgc ggcggtcggt
     4441 cccgacaaag tactcggtct gcttttgccc taccgaactt catcggcgga gtcgctgcac
     4501 gacgcccgtc tgatggtgga acggctcggc attccgcacc gggtcatcga catctcgccg
     4561 atgatcgacg cttactaccc acggatcacc gatgagctca agcgccgtgc cggcaacaag
     4621 atggcgcggg agcgcatggc tatcctgttc gacgtcgcgc tggagacctc gcggctggtt
     4681 ctcgggaccg gcaaccgcag cgaaattgcg ctgggctaca cgacctggca cggcgattcc
     4741 gcctgcagcg tcaatccgat cggcgagctg tacaagaccg aggtgcgcct cctcgcccgc
     4801 aagctggcgg tcccggaacc gatcatcgcc aagccgccca ccgcggattt gtggaaagat
     4861 cagaccgatg agggggagat cggcgtcacc tacccgcaga tcgaccgcct gctggcccgg
     4921 attgtcgacc agggcgttcg ttccatgagt caactggcgg cggagggttt cccgcccacc
     4981 gaaatatcgc gggtcgtgtc gctgctcaac cgcaacgcct tcaagcgcgc cttgccggag
     5041 gtcgcccccc tgcagcgaaa gccgatcccg gcgaacgtgc aactctcgga ataagggggt
     5101 cgttatgccc gagtcgcccg gcctgctcca tctggttccg acgccgagcg gcaacatggc
     5161 cgatctgacc cgtcgggcgg aagaaatgct ggccgccgcc gacctggtcg cctgcgagga
     5221 cacccggcac agcggccggc tcctcaaggc ccttggtctg aataagcggt tgatcagcta
     5281 ccatgatttc aatgagcgga cgcgcgccga gcagctcatc gacctcatcc ggcagggaca
     5341 gacggtggcg gtcatcaccg atgccggctc gcccggcatt tcggatccgg cctaccgcat
     5401 cgtgcgcacc gccatcgaca acggcattac gatcatcccc ttgcccggtc cgacctcgat
     5461 catcccggcc ctgacggcat cggggctgcc gaccgaccgg ttctttttcg aggggtttct
     5521 gccgcacaag tcgggagcgc gccgcacgcg gctggcccgg ctgaccgagt ttgaccacac
     5581 cctcgtcttc ttcgaatcgc cccatcgcgt ggtgcggtcg ctggaggaca tccgggag
//
LOCUS       DAOOKS010000021         5584 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_17432_length_5584_cov_3.686743, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000021 DAOOKS010000000
VERSION     DAOOKS010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5584
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17432_length_5584_cov_3.686743"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="PK112_00695"
     CDS             complement(<1..263)
                     /locus_tag="PK112_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HPC10601.1"
                     /translation="MMTAQESAPAPRTQPRRRAVDILGLLGWLILVFAVSFAASRYMP
                     GEWYARLHKPSWTPPNWMFPVVWTLLYAAMAAACSMLVWSGFCR"
     gene            356..1630
                     /locus_tag="PK112_00700"
     CDS             356..1630
                     /locus_tag="PK112_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010472222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPC10602.1"
                     /translation="MANHKDRSDRRRLRLGVVGGGAAGLFAAIAAAETGRPGEVIVWE
                     ATGAPLDKVRLSGGGRCNVTHRCFEPADLVKNYPRGERELRGPFARFQPRDMIAWLEG
                     RGVRLKAEADGRVFPASDDSGAVVACLLREAERAGVRIRLGSRVREAAPAGRDTDDAP
                     LFDVIAGDGSREQCDRLLIAAGGGRPGHALAQALGHTIVPPVPSLFTFTVPDLRLQGL
                     AGVSFESVQLRLAVGGERFEQSGPMLITHWGLSGPAVIRLSAWAARPLHESRYRAELV
                     VNFVPGSTAEQVYRGMTAHKQAHGKRLAHGDSPVSLPSRYWSRIAAAVGIPPETAWAA
                     VTREHMAVLAEQVTETHLAVAGRGIFKEEFVTCGGVALGEVDFRTMESRLRQGLYFAG
                     EVLDIDGLTGGFNLQSAWTTGWIAGRSMAGVK"
     gene            complement(1689..2768)
                     /locus_tag="PK112_00705"
     CDS             complement(1689..2768)
                     /locus_tag="PK112_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10603.1"
                     /translation="MNYRLAFILALLLPGMSAAARIVHNRTTLATGEDSLAVAFQVLD
                     SLGNPTEADSIFISVIGPSGGMTAVDSMTPADARVSVTAPGGQPAYVFHCQVSAIDGA
                     GAPGVYALTVMAKSTALGLATQTAAAFQIVAAPLSERLALLDDTVRVNGGVIDSNRTE
                     RGMAIGSGAVSVVLIAYDSTRTQVIPGVRVVIRDLAQTLLHGLGFTDVSGAAAFNLDA
                     DSVLAVAAAPGYLFPPFDTLAIVGATMDTLFGTASYPEPPPAGGLCRVWGCLYGIDGS
                     PQAGATISAMLPSGVARFGGAVVAPSPVSTATDSTGLFQLDLIPSELLESEDPRYDLT
                     IFRPDGVILRQRIAVPNQPLWQLEW"
     gene            complement(2795..3544)
                     /locus_tag="PK112_00710"
     CDS             complement(2795..3544)
                     /locus_tag="PK112_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10604.1"
                     /translation="MTRSAAVLLAALTPLLAEAARIDTLRGPSQIEDATIYGYLTCDG
                     EISGEDCRRYNTGNCLYFSVGTQGTGNHRAVLRFPGWDGVVPDSSVLELFCELEDDGA
                     DRRIFLYPLTRRFVEGVEGFGAGGIPADSGVTWNHAWLAHGRGDSLSWTSPGGDFTAA
                     VACTLIAADSGKYQRVAGFNRMLAYWDSTGSDPGVILIGENVFPANTAKKTFTSAEGA
                     GDRAPRLLLYYPGAPVAAGRRRLQSTAAPAR"
     gene            complement(3541..3882)
                     /locus_tag="PK112_00715"
     CDS             complement(3541..3882)
                     /locus_tag="PK112_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10605.1"
                     /translation="MAPGAQIRERLLCEWLARLELLIAGHFDALLGFARPKSRSRSGG
                     ASGPPRSAQNAKVSELIKMIQLYARLADSRPGTDEFWEMIERIRQEKLPLRPTGQLPA
                     AEASPKKGRRP"
     gene            complement(3884..4258)
                     /locus_tag="PK112_00720"
     CDS             complement(3884..4258)
                     /locus_tag="PK112_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10606.1"
                     /translation="MLTPLNDVLARRLWFIPGFVVWGVPGLSEIDFVAAEQIGGATAL
                     RHGAAVPGGGPQTVLYADLVDHRGNALPAALASPRVWLRPQGATPAFVVGAETPSSFR
                     VARDPDGAGPVTADLFVIEMGD"
     gene            complement(4270..5082)
                     /locus_tag="PK112_00725"
     CDS             complement(4270..5082)
                     /locus_tag="PK112_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10607.1"
                     /translation="MSYTTDELVRRHLADTRPLADRVTDQAIVLGESGWVLFWRGPIE
                     AGTVVVKALRGAAPLRRALALAGGSAALGVAPVVPGSVVAASDSSLGTIYAENVDFVI
                     DYPRAVFYAKAGGALADDASVTVWLREYTLCAPGLDYALDAEAGALRRLAGGSIAPVE
                     TVRLDYTPLYAAGDDMLIAAAVAEANGLVGAAVDPARAFGADPALEAAATCHALAVVC
                     RGSAARVLATRPEQERAAAAWTRLSELYADRADRLLAAFHPPAAGPAAPSAV"
     gene            complement(5086..>5584)
                     /locus_tag="PK112_00730"
     CDS             complement(5086..>5584)
                     /locus_tag="PK112_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10608.1"
                     /translation="APAALDARAAPFAAVALYDTVSASWANLTAEATGTAPADIAHPV
                     SGALLAHAGDALYLGMEERFRFAKVRLSSAGAGGSTVCSYWDGSAWAAITPASGPYNF
                     DVLEHDLLLWNDYNTVPVDWQKKPVAGAVHFWVKIEALADFAAAPVGSQITAVSRVTA
                     LTVRR"
BASE COUNT          870 a         1839 c         2083 g          792 t
ORIGIN      
        1 cggcagaatc ccgaccacac gagcatagag cacgccgccg ccatcgccgc atacagcagc
       61 gtccacacca ccgggaacat ccagttcggg ggagtccacg acggtttgtg cagtcgggcg
      121 taccactcgc ccggcatgta ccgggaggcg gcgaacgaca ccgcgaacac gaggatcaac
      181 catcccagca acccgaggat atcaaccgcc cgccggcggg gctgagttcg gggagcgggg
      241 gcgctttcct gggcagtcat catgcgacct ccatcgccgc ggaccgaccg gtccgctccg
      301 cgcggggcgg agtttgggct tgtaaccgct tcctccggat acaatactac ctcgcgtggc
      361 gaatcacaag gacagatccg accggcgccg gctgcggctg ggggttgtcg gcggaggggc
      421 ggccggcctc tttgcggcga tcgcggccgc cgaaaccggc cggcccggcg aggtgatcgt
      481 gtgggaggcg accggcgcgc cgctcgacaa ggtccgcctg tcgggaggcg gacggtgcaa
      541 cgtcacgcac cgttgtttcg agcccgcgga tttggtgaag aactacccgc gcggggagcg
      601 cgagctgcgc gggccgttcg cgcggttcca gccgcgcgac atgatcgcct ggctcgaggg
      661 ccggggggtg cgcctgaagg cggaggcgga cgggcgagta tttccggcga gcgatgattc
      721 gggcgcggtg gtcgcctgcc tgctgcgcga ggcggagcga gccggcgtgc ggatacggct
      781 ggggtcgcgt gtgcgcgagg cggcgccggc gggaagggac accgacgatg cgccgctgtt
      841 cgatgtcatc gcgggcgacg ggagccgaga gcaatgcgac cgcctgctga tcgccgccgg
      901 cggcggtcgg ccggggcacg cgctggctca ggcgctcggc cacaccattg ttccccccgt
      961 gccgtcgctg ttcaccttca ccgttcccga cctgcgcctg caggggctgg cgggagtcag
     1021 tttcgaatcc gtgcagttgc ggctcgcagt gggaggggaa cggttcgaac agagcggacc
     1081 gatgctcatt acccattggg ggctgagcgg gccggctgtg atccggctct ccgcgtgggc
     1141 ggcgcgtccg cttcacgaga gccgttaccg cgcggaactg gtggtcaatt tcgtccccgg
     1201 ttccacggcc gaacaggtct accgcggtat gacagcgcac aagcaggcgc acggcaagcg
     1261 gctggcgcac ggcgacagcc ccgtctcgct cccctcgcgc tactggagcc ggatcgcggc
     1321 cgcagtcggg atcccgccgg agaccgcgtg ggcggccgtc acgcgggagc acatggcggt
     1381 cctcgccgag caggtgacag agacgcatct ggccgtggcc gggcggggga ttttcaaaga
     1441 ggaattcgtc acctgcggcg gggtggcgct gggggaggtc gatttccgga cgatggagag
     1501 ccggttgcgg caggggctgt atttcgccgg ggaggtgctc gatatcgacg gtctcacggg
     1561 ggggttcaat ctccagagcg cctggaccac cggctggatc gccggccgga gcatggcggg
     1621 ggtgaagtag gggaagggca cggccaggcg gcggccatgc ccctctcttg gcgctgaagg
     1681 gcgcggtcct accactccaa ctgccagagc ggctgattcg ggacggcgat ccgctggcgg
     1741 aggatgacgc cgtcgggccg aaagatcgtc aggtcatagc ggggatcctc gctctccagc
     1801 agttccgacg gaatcaggtc cagttgaaag aggccggtgc tgtcggtggc ggtgctcacc
     1861 gggctgggcg ccacgaccgc accgccgaag cgcgcgacgc ccgaggggag catcgcggag
     1921 atcgtcgccc cggcctgcgg cgatccgtca atgccgtaca ggcagcccca gacacggcac
     1981 aggccgcccg cgggcggcgg ttcggggtac gaggccgtcc cgaacagcgt gtccatggtc
     2041 gcgccgacga tcgccagcgt gtcgaacggc gggaagaggt aaccgggagc ggccgcgacc
     2101 gcgagcaccg agtcggcgtc gagattgaag gcggctgcgc cgctcacgtc ggtgaacccg
     2161 aggccgtgca ggagcgtctg ggcgagatcg cggatgacga cgcggacgcc ggggatgacc
     2221 tgggtgcggg tcgagtcgta ggcgatgagc acgaccgaca cggcgcccga gccaatcgcc
     2281 atcccgcgct cggtgcggtt gctgtcgatc actccgccgt tcacccggac ggtatcatcg
     2341 agcagggcga gccgttcgga caacggcgcg gcgacgatct ggaaggccgc cgccgtctgg
     2401 gtcgccagcc cgagggccgt cgatttggcc atcaccgtca gcgcgtaaac gcccggtgcg
     2461 ccggcgccgt cgatggccga gacctggcag tggaacacat aggcgggctg accacccggc
     2521 gctgtcaccg agacccgcgc gtcggccggc gtcattgagt cgaccgccgt catgccgccc
     2581 gacggaccga tcacggagat gaagatcgaa tcggcctcag tcgggttgcc gagactgtcg
     2641 agcacctgga acgccacggc gagcgaatcc tcaccggtcg caagcgtcgt gcggttgtgg
     2701 acgatccggg cggcggccga catccccggc agcagcagcg cgagtataaa agcaagccga
     2761 taattcatgg gcacgttcct ttctcgtcgg atcatcagcg cgcgggggcg gctgtcgact
     2821 gcagccgccg ccgtccggcg gcgaccggag cgccgggata gtacaacaac agccgcggcg
     2881 cccggtcgcc ggcgccttcg gccgaggtga aggtcttttt cgccgtgttg gcggggaaga
     2941 cgttttctcc gatcaggatg acgccgggat cggacccggt ggaatcccag taggcgagca
     3001 tgcggttgaa accggcgacg cgctggtatt tgccgctgtc ggcggcgatc agcgtgcacg
     3061 ccacggctgc ggtgaaatca cctcccggcg aggtccagga gagcgagtcg ccccggccgt
     3121 gagccagcca ggcgtggttc caggtgacgc cgctgtcggc gggaatgccg cccgcgccga
     3181 atccctcgac gccctccacg aagcggcggg tgaggggata gaggaagatc cgccggtcgg
     3241 cgccgtcgtc ctccagttcg cagaacaatt ccaggacgga ggaatccggc acgacgccgt
     3301 cccatccggg aaagcgcagc accgcgcgat ggttgccggt gccttgggtt cccacggaga
     3361 aatagaggca gttgcccgtg ttgtagcgcc ggcagtcctc gccgctgatt tcgccgtcgc
     3421 aggtaaggta gccgtagatg gtggcgtcct caatctgcga cggtccgcgc agcgtgtcga
     3481 tgcgcgcggc ctcggcgaga agcggcgtca gcgccgcgag cagcacggcc gccgagcgcg
     3541 tcatggccgg cgtcctttct tggggctcgc ctccgccgcg ggaagctgcc cggtcggccg
     3601 gagcggcaat ttctcctggc ggattcgttc gatcatctcc cagaactcgt cggttccggg
     3661 ccggctgtcg gccagccggg catacagttg gatcattttg atcaactccg atactttggc
     3721 gttttgggcg ctgcgcggcg ggccggacgc gccgccggag cggctccggc ttttcggccg
     3781 ggcgaatccg agcagggcgt cgaagtgccc cgcgatgagc aattcgaggc gcgcgagcca
     3841 ttcgcaaagg agccgttcgc ggatctgggc gcccggagcc atctcagtcg cccatttcga
     3901 tgacgaagag atcggcggtc accggcccgg cgccgtcggg atcgcgcgcg acccggaagg
     3961 acgagggcgt ttcggcgccc acgacgaagg caggcgtcgc gccctgcggc cgcagccaca
     4021 cgcgcggcga tgccagcgcc gccggcagag cgttcccccg gtggtcgacg aggtcggcgt
     4081 agagcaccgt ctgcggtccg ccgccgggga cggcggcgcc gtggcggagc gcggtcgcgc
     4141 cgccgatctg ctcggcggcc acgaagtcga tttcgctcag ccccggcacg ccccagacga
     4201 cgaatccggg gataaaccac aggcggcggg ccagcacgtc gttaagcgga gtcagcatgg
     4261 ttcctccttt cacacggccg agggcgcggc gggaccggca gccggcggat ggaaggccgc
     4321 gagcagacgg tcggcgcggt cggcgtagag ttccgagagc cgggtccagg cagcggccgc
     4381 tctctcctgt tcgggacggg tggcgagcac gcgggcggcc gatccgcggc agacgacggc
     4441 gagggcatga caggttgccg ccgcctcgag ggcgggatcg gcgccgaacg ccctggccgg
     4501 gtcgaccgcc gcacccacca ggccgttggc ctcggccact gccgccgcaa tcagcatgtc
     4561 gtcgccggcc gcgtagagcg gcgtgtagtc gaggcggacg gtttcgacgg gggcgatgga
     4621 cccgccggcg agccgccgga gcgcccccgc ctcggcatcg agggcgtagt cgaggccggg
     4681 cgcgcacagc gtgtactcgc gcagccagac ggttaccgac gcgtcgtcgg ccagcgcgcc
     4741 gccggctttg gcatagaaaa cggcgcgggg ataatcgatg acaaaatcga cgttttcggc
     4801 gtaaatcgtc ccgagcgagc tgtcggaggc ggccacgacc gaccccggca cgaccggggc
     4861 gactcccagc gccgcgcttc cgcccgcgag ggcgagcgcg cgccggagcg gcgccgcgcc
     4921 gcggagggct ttcacgacga ccgtccccgc ctcgatcggt ccgcgccaaa aaagaaccca
     4981 gccgctttct cccaggacga tcgcctggtc ggtcacccgg tcggccagcg gccgggtgtc
     5041 ggcgaggtgg cggcgcacca gttcatcggt ggtgtacgac actgttcacc tcctcaccgt
     5101 gagggcagtc acgcgggaga cggcggtgat ctggctgccc accggggcgg ctgcaaaatc
     5161 ggcgagcgcc tcgattttca cccagaagtg taccgcgccc gcgaccggct tcttctgcca
     5221 gtcgaccggc acggtgttgt agtcgttcca caggagcagg tcgtgctcga gtacgtcgaa
     5281 gttgtacggg ccggaggcgg gcgtgatggc cgcccacgcg gaaccgtccc agtagctgca
     5341 gacggtgctg cctcccgccc cggcggagga cagccgcact ttcgcgaagc ggaagcgctc
     5401 ctccatgccg aggtagaggg cgtcgcccgc gtgggcaagc agggcgcccg agaccggatg
     5461 ggcgatatcg gcgggcgcgg tcccggtagc ctcggcggtg aggttcgccc acgaggcgct
     5521 caccgtgtcg tacagggcga cggcggcgaa gggggcggcg cgggcatcga gcgccgccgg
     5581 cgcg
//
LOCUS       DAOOKS010000022         5514 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_17734_length_5514_cov_3.052391, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000022 DAOOKS010000000
VERSION     DAOOKS010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5514
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17734_length_5514_cov_3.052391"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="PK112_00735"
     CDS             complement(<1..258)
                     /locus_tag="PK112_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10609.1"
                     /translation="MERREFLKRTGRAMVLAAASGGIGVLFHNRNAAGYRPLAVFDRR
                     FTVFGDPACPAVAFARHPDPEHALDAALAALAAHPDRRDVRL"
     gene            399..1178
                     /locus_tag="PK112_00740"
     CDS             399..1178
                     /locus_tag="PK112_00740"
                     /inference="COORDINATES: protein motif:HMM:NF018024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HPC10610.1"
                     /translation="MNRRLVSAAWAVAALVAGAAAPAEATFSIVAVDTVNGVIGGAGA
                     SCIDNCFIIDDIIESIGAVHTQAYWLQGNKNYAHQLLAQGLTPDSIILLLVQNDVEGT
                     PEYRQYGVVTLAGSGASAAYTGSATSDWAGHLTGPGYAVQGNILLGPEVIADMETAFL
                     GTDGPLEAKLMAAMEAAKRPGADNRCIFAGKSAISAFIKVVRPGDGGTPFLQEIVPTT
                     TGSTDPIDVLHAQFDTWRLRQAAHPDSSTVVIGKTALDGDG"
     gene            1245..1628
                     /locus_tag="PK112_00745"
     CDS             1245..1628
                     /locus_tag="PK112_00745"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HPC10611.1"
                     /translation="MRVGTTGQAVLSAVADNGDGTFACVLTAPLYGAPDDTLTVTVGA
                     YYDFVTELHSRPAVTYYYCGDVTGDGSIKVSDLTFFVNFKFRGGPGAAFPSSADMNGD
                     GQVKISDVTYIVAYLFAGGPPPICE"
     gene            complement(1669..2664)
                     /locus_tag="PK112_00750"
     CDS             complement(1669..2664)
                     /locus_tag="PK112_00750"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10612.1"
                     /translation="MRHHAVLQGTYAQTTLLRSALLKAARAWFEREHFLEICVPHITG
                     ATGSCEWFPNAMPVTMYDPAGAETPMFLRQTGQLYLEAFTQAHNRVYTIGPSFRQERK
                     VTDRHLCEFTLIEFEGRDFELDGLMNHIEGLVKTMYRAARRVMPTDRLTRYIRSPFNR
                     ITYREAVRLLCARGHGLTYGDDLGSAEEKALCAHLGGLPTFVTHYPADPRPRPGGVIK
                     FFSMKRREGETLCCDLLLPFVGESVGGAVREGCAETCRRQFRESYMYDHIVGAGIDPR
                     QFDWYFEVLSHGTGQSAGCGIGFERVVQSILATRQQEVTIKGAVELPRSPDFLVP"
     gene            complement(3017..3532)
                     /locus_tag="PK112_00755"
     CDS             complement(3017..3532)
                     /locus_tag="PK112_00755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HPC10613.1"
                     /translation="MMKPVVMGMGNELFGDDGFGIIAARRLAPLAGEAADVIECGAAG
                     LALLDPLIGRPRAVIIDAICTGRQAPGTIYRVSPADLRDITNLSPHYAGLPEVIRMAQ
                     RLELPIPDTIDIIAIEAADITTMGAGLSPAVAAALEPVVALAVELLLGGRRTGSPAAD
                     AQGISAAGTHV"
     gene            complement(3532..5001)
                     /locus_tag="PK112_00760"
     CDS             complement(3532..5001)
                     /locus_tag="PK112_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012309762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HPC10614.1"
                     /translation="MTTTRRVTIDPITRLEGHGKIDILLNDRGDVDHAYFQVPELRGF
                     ERFAHGRPAEDMPQITSRICGVCPTAHHMASTRTLDDLYKVEPPPAAHKVRELLYNIF
                     FVEDHALHFFYLAGPDFVVGPTAPKAERNIIGLLGKVGIEAGKKVIGMRRDLRELMVL
                     AMGKAAHPAFGLPGGISRPIPPEHQKAFIEVCERAVEFAQFSLKVFDDVVLANKTYVD
                     YITSDTYTHRTHYMGLVDAHNRPNYYRGELRVVGPDGREVCKFPVAKYLDHIAEHVEP
                     WSFMKFSYLKDVGWKGFVDGPDSGIIAVAPLARLNAAEGMPTPLAQKAFERFYQTFGG
                     RPVHHTLANHWARLIELLCAAERARELSRDPEIVSKDVRVLPTKTPSEGIGVVEAPRG
                     TLIHHYRTDERGVIKMANLIVATQNNAARISLSVDKAARGVISGGRIDDGVLNLVEMA
                     FRAYDPCNGCATHTLPGSMPLVVRVYDAAHNQVQEIRRD"
     gene            complement(4998..>5514)
                     /locus_tag="PK112_00765"
     CDS             complement(4998..>5514)
                     /locus_tag="PK112_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HPC10615.1"
                     /translation="AVDYFLPGCPPPVKLILGAVEAILAGKLPAKGSTLAPDMALCDE
                     CPRKETKPEKLTVKEFHRPHEILIDPEQCLLAQGLLCLGPVTRQGCGGACTKVSMPCT
                     GCLGPTSRITDFGAKAMSAMASIIEAKDDAGIEATLARIVDPAGTFFRYSLPASLLHR
                     KYEPETTRGMK"
BASE COUNT          900 a         1825 c         1893 g          896 t
ORIGIN      
        1 cagacggacg tcgcgccggt ccgggtgcgc cgctagcgcg gccagggcgg cgtccagggc
       61 gtgctcgggg tcggggtggc gggcaaaagc gaccgcgggg caggccgggt cgccgaaaac
      121 cgtgaaccgg cggtcgaaca ccgcgagcgg tctgtaaccc gcggcgttgc ggttgtggaa
      181 gaggacgccg atgccgcccg aggcggccgc cagcaccatc gcccggcccg ttcgcttgag
      241 gaactcgcgt cgctccatga agatccctcg gtcacccgac cttttccgcc aatatacgcc
      301 gggggaacgc gcgcggcaag aagaggcctt gacgaatcgc gaaatccgcg catattggag
      361 aaaaggtccc atcaccgtcc gcttgcccag gagtgcgtat gaatcggaga ctggtgtcgg
      421 ccgcgtgggc ggttgcagcc ctcgtcgcgg gagccgccgc cccggcggaa gcgacgtttt
      481 caatcgtggc cgtcgatacc gtcaacggcg tcattggcgg cgccggagcc tcctgcatcg
      541 acaactgctt catcatcgat gacatcatcg aatcgatcgg cgcggtccac acccaggcct
      601 actggctgca gggcaacaag aactacgccc accagttgct cgcccaggga ttgacgcccg
      661 attcgattat cctcctgctc gttcagaacg atgtcgaagg gacgcccgaa taccgccagt
      721 acggcgtcgt caccctggcc ggatccggcg cctcggccgc ctacaccggc agcgctacca
      781 gcgactgggc cggtcacctc accggccccg gctacgctgt ccaggggaac atcctcctcg
      841 ggccggaggt gatcgccgac atggagacgg cgtttctggg aactgacggc ccgctggagg
      901 cgaaactgat ggcggcgatg gaggcggcca aacggccggg ggcggacaac cgctgcatct
      961 tcgccggcaa gtcggcgatc tcggcattca tcaaggtcgt gcggccgggc gacggcggca
     1021 cgcctttcct ccaggagatc gtccccacca ctaccggatc gaccgacccg atcgatgttc
     1081 tgcacgccca gttcgacacc tggcgtctgc gccaggctgc ccacccggat tcctcgacgg
     1141 tggtgatcgg gaagactgcg ctcgacggcg acgggtaggc gtcgacggtc atcaccattg
     1201 ctccgcgcaa ctgggccggg gcgcccccgg tccggatcga ctcggtgcgg gtgggcacga
     1261 cgggccaggc ggttctgagc gcggtcgccg acaacggcga cggaaccttc gcgtgtgtcc
     1321 tgaccgcccc cctctacggc gcccccgacg ataccctcac cgtgaccgtg ggggcctact
     1381 atgatttcgt caccgaactg cactcccgcc cggccgtcac ctactactac tgcggcgatg
     1441 tgaccggcga cggcagcatc aaggtctccg acctgacgtt ctttgtgaac ttcaagttcc
     1501 ggggcggtcc gggagccgct ttcccctcct ccgccgatat gaacggggac gggcaggtga
     1561 aaatcagcga cgtcacgtac atcgtggcgt acctgttcgc cggcggtccg ccgccgatct
     1621 gcgaatagcc ggccgcctcc ccggcgcggg ccgtcagcag gacggtcgtc aggggacaag
     1681 aaaatcgggc gagcggggga gttccaccgc ccccttgatg gtcacctcct gctggcgggt
     1741 ggccagaatc gactgcacga cgcgttcgaa tccgatgccg cacccggcgc tctggccggt
     1801 gccgtgcgag agcacctcga agtaccagtc gaactgccgc gggtcgatcc cggcgccgac
     1861 gatgtggtcg tacatgtacg attcgcggaa ctgccggcgg caggtctcgg cgcatccctc
     1921 gcgcacggcc ccgccgacgg actcgccgac gaaggggaga agcaggtcgc agcagagcgt
     1981 ctcgccctcc cgccgcttca tcgaaaagaa cttgatcacg ccgccgggcc gcggcctggg
     2041 atcggccggg tagtgggtga cgaaggtcgg cagaccgccg aggtgggcgc acagcgcctt
     2101 ctcctcggcg ctcccgaggt cgtcgccgta agtcagcccg tggccgcggg cgcaaaggag
     2161 ccgaaccgcc tcacggtagg tgatgcggtt gaagggagac cggatgtagc gggtgagccg
     2221 gtcggtcggc atgaccctgc gagcggcccg gtacatcgtc ttgaccaaac cttcgatgtg
     2281 gttcatcaga ccgtccagct caaagtcacg cccctcgaac tcgatgaggg tgaactcgca
     2341 caggtggcgg tcggtcactt tgcgctcctg gcggaacgac ggtccgatcg tatagacgcg
     2401 gttgtgggcc tgggtgaacg cctcgaggta gagctggccg gtctggcgga gaaacatcgg
     2461 cgtctcggcg cccgcgggat cgtacatggt gaccggcatg gcgttgggga accactcgca
     2521 cgagccggtg gcgccggtga tgtgggggac gcagatctca agaaagtgct cgcgctcgaa
     2581 ccaggcgcgg gcggccttca aaagcgcgga acgcagaagc gtggtctggg catacgtgcc
     2641 ctgcaggaca gcgtggtgac gcatacgggc ctccatggca tacggcggtg ataaaaaaag
     2701 gccgcccgga aagccgggtg gccgatagtt ggcgtgcggg aaagaaaacc cccgcggcgc
     2761 gggcggcggt cattggaccg cctcgcgcgc ccttgccgca gggcggcccg tcgggcgccg
     2821 gtccggtctc ttcagcggca ggcgcatagg ctgcggtcgc ggaccgggcg caagcgcgcc
     2881 ccggccgccg atgaggaaac gctttgatct gtgcggaaat aatacattct ggagcttaac
     2941 ggtacatgtt ccccgccgcc gtgtcaagcg gaaaataaaa aaaaccgccg cccgccccgc
     3001 cgcgcgggcg cgggtttcag acgtgtgtgc cggccgcgga gatcccctgc gcgtccgccg
     3061 ccgggctgcc cgtccgccgc ccgcccaaca ggagctcgac cgccagggcg acgaccggtt
     3121 cgagagctgc cgcgaccgcg gggctcagac cggcgcccat ggtcgtgatg tccgccgcct
     3181 cgatcgcgat gatatcgatc gtgtcgggta tcggcagctc cagccgctgc gccatgcgaa
     3241 tgacctcggg cagtccggca taatgcggtg agaggttggt tatgtcccgc aagtctgccg
     3301 gactgacccg atatatcgtg cccggggcct gccgccccgt gcaaatcgca tcgattatca
     3361 cggcccgggg acgcccgatg agcgggtcca gcagcgccag accggcggcg ccgcactcga
     3421 tcacatcggc ggcctcgccc gccagggggg ccagacggcg ggcggcgatg atgccgaacc
     3481 cgtcgtcgcc gaacagctcg ttccccatgc ccatgacaac cggtttcatc atcagtcgcg
     3541 ccgaatctcc tgaacctggt tgtgggcggc gtcgtacacg cgcacgacga gcggcatcga
     3601 ccccggaagg gtgtgggtgg cgcagccgtt gcacggatcg taggcccgga aggccatctc
     3661 gacgaggttg aggacgccgt cgtcgatgcg gccgccggag atcacgccgc gcgcggcctt
     3721 gtccaccgac agggagatcc gggcggcatt gttctgggtc gccacgatga ggttggccat
     3781 tttgatcacg ccccgctcgt cggtccggta gtggtgaatg agggtgccgc gcggcgcttc
     3841 gaccacgccg atcccctcgc tcggcgtctt ggtcgggagc acccgcacat ccttgctcac
     3901 gatctcgggg tcgcgggaca gctcgcgcgc ccgctcggcg gcgcacagca gctcgatcag
     3961 gcgggcccag tgattggcga gcgtgtggtg caccggccgc ccgccgaacg tctggtagaa
     4021 ccgctcgaag gctttctgcg ccagcggcgt cggcatcccc tcggcggcgt tgagccgggc
     4081 cagcggagcc accgcgatga tgccgctgtc gggaccgtcc acgaacccct tccacccgac
     4141 atctttcagg taggagaact tcatgaagct ccacggctcg acgtgttcgg cgatgtggtc
     4201 gaggtatttg gccaccggga acttgcacac ctcgcggccg tcgggaccga ccacccgcag
     4261 ttcgccgcgg tagtagttgg ggcggttgtg ggcgtcgacc aggcccatgt agtgggtccg
     4321 gtgagtgtag gtgtcggagg tgatgtagtc gacgtacgtc ttgttcgcca gcacgacgtc
     4381 atcgaacacc ttcaggctga actgggcgaa ttccaccgcc cgctcgcaca cctcgatgaa
     4441 cgccttctgg tgctccggcg ggatcggccg ggagatgccg ccaggcagac cgaaggccgg
     4501 gtgggcggct ttccccatcg ccagcaccat cagctcccgc aggtcccgcc gcatcccgat
     4561 caccttcttg cccgcttcga tccccacttt cccgagcagg ccgatgatgt tgcgctcggc
     4621 cttgggggcg gtgggcccga ccacgaaatc cggccccgcg aggtagaaga agtgcagggc
     4681 gtggtcctcg acgaagaaaa tgttatagag cagctcgcgc accttgtggg cggccggggg
     4741 cggctccacc ttgtacaggt cgtcgagcgt ccgcgtggag gccatgtggt gggcggtcgg
     4801 gcagacgccg cagatgcggg aggtgatctg cggcatgtct tcggccggac gcccgtgggc
     4861 gaaccgctcg aacccgcgca gttcgggcac ctggaaatag gcatggtcga cgtccccccg
     4921 gtcgttcagg agaatgtcga tcttgccgtg cccctcgagc cgcgtgatcg ggtctattgt
     4981 cacccgtcgt gtcgtcgtca tttcatcccc cgcgtggttt cgggttcata cttccggtgg
     5041 aggagcgagg ccggcaggct gtagcggaag aaagtgccgg cggggtcgac gatcctggcg
     5101 agcgtcgctt cgatgccggc gtcgtctttc gcctcgatga tggaggccat cgccgacatc
     5161 gccttggcgc cgaaatcggt gatccggctg gtcggcccga ggcagccggt gcagggcatg
     5221 ctgaccttgg tgcaggcgcc gccgcagccc tggcgcgtga ccggcccgag gcacaggagc
     5281 ccctgggcga gcaggcactg ctcgggatcg atgagaatct cgtgcggacg gtggaactcc
     5341 ttcaccgtca gtttctccgg cttggtctcc ttgcggggac actcgtcgca cagcgccatg
     5401 tccggcgcga gcgtgctccc cttggccggg agcttccccg cgaggatcgc ttccaccgcg
     5461 ccgagaatca gcttcaccgg cggcggacag ccggggagaa agtagtccac ggcg
//
LOCUS       DAOOKS010000023         5502 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_17778_length_5502_cov_2.699468, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000023 DAOOKS010000000
VERSION     DAOOKS010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5502
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17778_length_5502_cov_2.699468"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /gene="pyrH"
                     /locus_tag="PK112_00770"
     CDS             <1..294
                     /gene="pyrH"
                     /locus_tag="PK112_00770"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683263.1"
                     /note="Catalyzes the phosphorylation of UMP to UDP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HPC10616.1"
                     /translation="TAASLRAMEVGADLMIKATNVDGVYSADPRKDAKATFYPRLSYM
                     DILTRELRVIDATAISLLKENQIPLRIVDLHRPGNLRRTVLGEDVGTLVTSAG"
     gene            326..883
                     /gene="frr"
                     /locus_tag="PK112_00775"
     CDS             326..883
                     /gene="frr"
                     /locus_tag="PK112_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009611042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HPC10617.1"
                     /translation="MVDKIEKDAHDRMHKTIETLKREFGTVRTGKASPHLLDTVTVEA
                     YGTQMPLNQVATVTAPEPRLLVVTPFDKSTVGNVVKGIQAADLGFNPMVDGQIIRVPI
                     PALNEERRRTLVKQCKHLAEEARVAVRNIRRDANEYLKQAEKDSKISEDEMRKSLDKV
                     QKLTDEHIKEIDSLLTVKEKEVMEV"
     gene            complement(1006..2817)
                     /locus_tag="PK112_00780"
     CDS             complement(1006..2817)
                     /locus_tag="PK112_00780"
                     /inference="COORDINATES: protein motif:HMM:NF025067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HPC10618.1"
                     /translation="MKLPSLAQLAAEYGRTIRRFPLVLVCALLGTLAALILVDQEGSR
                     EASVLYNILLTAILALPVMTAVKLAAERSSAPASSAWPAQAAAAALLVVYAATVPTDL
                     PRAPLVHLLRFFALGIGCTLLASVWPFRQKGEDNRFWHFTSLVVFRIMLAGAFALVLF
                     AGLALALAALDNLFGMAIPSKRYAELWILTLGLFAVPYVLAGLPEDWSALGTGSDYPR
                     PLKVLGQYVLPPLVAVYFVILYAYIAKIIVTWSWPQGWVGRLIIGFSATGILALSVLD
                     PMRERTESLWIKRASRWFYLILLPLIVVLFLALWRRISEYGLTEGRYLGLAVGVWLAV
                     IAAYFLLSRCKSIRMIPASLCAFAFLISVGPWGMFSVSEQSQIRRLRGMLTADSILVN
                     GAVTKAPAPVPADHEVQISAILIYLYEVHGFSALEPWFGETLRADTEDAGNRFKGPEY
                     VAELLGIEFHPYDTPGREQYFAVRIDPQTPISVAGYDHMLRAGYGGRAAAPSPAGEPV
                     AGAIETAADSVVVRFVLDSTTTDSVTVSLRPLIDRIVAARADARGSEISGEEAMIENE
                     TGGVKVKVVMLQADIRRDDSITVLNSYDALILYSRRK"
     gene            3288..3662
                     /gene="acpS"
                     /locus_tag="PK112_00785"
     CDS             3288..3662
                     /gene="acpS"
                     /locus_tag="PK112_00785"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HPC10619.1"
                     /translation="MIQAIGLDIVEVARMERDLRTWGNRFAERILGAEELAILNRRHD
                     KSVFLAGRFAAKEAVIKALGYRLSERPAYRAIAITNDASGQPVLSLPDDIRAALGRAR
                     CFLSITHERTHAAAVAVITEDS"
     gene            3659..4237
                     /gene="pyrE"
                     /locus_tag="PK112_00790"
     CDS             3659..4237
                     /gene="pyrE"
                     /locus_tag="PK112_00790"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HPC10620.1"
                     /translation="MSQDDVVNLFKESGALLTGHFKLTSGRHSDVYYEKFTLLKQPAL
                     CTRLCARMADTLRTTGVRTIVGPTTGGIILAYDVARYLGVEAIYAEAADDGKGRVFKR
                     GFHLEPGQKVAIVDDVLTTGTSVDEVIALVRRYGAEIVGLRLMLDRSNGKVRFDYPFA
                     ALATVAAESWDPAECPLCAKAMPLTQRGSRKF"
     gene            complement(4290..4760)
                     /locus_tag="PK112_00795"
     CDS             complement(4290..4760)
                     /locus_tag="PK112_00795"
                     /inference="COORDINATES: protein motif:HMM:NF014629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HPC10621.1"
                     /translation="MLNITVLAVGRDKDRWVSEAVAHYEKLLARWCRLAWRIVPSPKH
                     SAALSPEEIRRAESARLRAALGPGYIAALAASGRTVDSPAFARLLERWHTVSGSNLTF
                     VIGGPHGLDRDLLSRADTVLSLSPLTFSHQLVRLILLEQLYRAFSILHGTDYHK"
     gene            complement(4753..5112)
                     /locus_tag="PK112_00800"
     CDS             complement(4753..5112)
                     /locus_tag="PK112_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HPC10622.1"
                     /translation="MIDVTAQVRRAVRESGVASGIAVCFVPHTTAAVTINENADPDVA
                     RDILYKLDKEIPAADGYRHAEGNSDAHVKSTLVGVSAHVLIEGGDLVLGAWQGIYFCE
                     FDGPRRRHLIVRVMSGA"
     gene            complement(5132..>5502)
                     /locus_tag="PK112_00805"
     CDS             complement(5132..>5502)
                     /locus_tag="PK112_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004850924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HPC10623.1"
                     /translation="LLVDTAGRLHTKSNLMEELKKIRRVIAKAAPEAAISTRLILDGS
                     TGQNALSQVRVFTDAVGCDGLIVTKLDGTAKGGVMIAVAQELNVPVDFIGLGEGIDDI
                     QRFNPREYTEALFADEPAKR"
BASE COUNT         1064 a         1708 c         1849 g          881 t
ORIGIN      
        1 accgcggcct cgctgcgcgc catggaggtg ggggcggacc tcatgatcaa ggcgaccaat
       61 gtcgacgggg tgtactcggc cgatccgcgc aaggacgcga aggcgacgtt ctacccccgg
      121 ctgtcgtaca tggacattct cacgcgggag ctgcgggtga tcgatgccac ggcgatctcg
      181 cttctgaaag aaaaccaaat ccccctgcgt atcgtggacc tgcaccgtcc cggcaatctc
      241 cgccgcacgg tgctcgggga agacgtcggg acgctggtga cgtcggcggg ataacacgca
      301 acgtgccatt gagggaggca ccgatatggt tgacaagatc gagaaagacg cccacgaccg
      361 gatgcacaag acgatcgaga cgctcaagcg cgaattcgga accgtccgca cgggaaaggc
      421 ctcgccgcac ctgctggaca ccgttactgt cgaagcttac ggcacccaga tgcccctcaa
      481 ccaggtggcc acggtgacgg cccccgagcc gcggctgctg gtggtgacgc cgttcgacaa
      541 gtcgacggtc ggcaacgtgg tcaaggggat ccaggccgcc gacctcgggt tcaacccgat
      601 ggtggacgga caaatcatcc gcgtgccgat cccggcgctc aatgaggaac ggcgcagaac
      661 gctggtgaaa cagtgcaagc acctggcgga agaggcccgc gtcgccgtgc gcaacattcg
      721 gcgcgacgcg aacgaatatc tcaaacaggc ggagaaggac tcgaaaatct ccgaggatga
      781 gatgcgcaag agcctggaca aggtgcagaa gctgaccgat gagcacatca aagagatcga
      841 ttctctcctg acggtcaagg agaaggaggt catggaggtc tagtatccgt ccatgcaaag
      901 cattgacagg cagaaggtta caccctcctg ccttcttttt ttttcgggga gacgggcccg
      961 cccggacctc gcggcgcgcg gccgcgaacc ggccaaacgc cgccgttact tcctgcgcga
     1021 atagagaatc agggcatcgt acgagttcag cacggtgatg ctgtcgtccc ggcggatgtc
     1081 cgcctgcagc atgaccacct tgaccttgac tcctcccgtt tcgttttcga tcatcgcctc
     1141 ctcgccggag atctccgaac cgcgggcgtc tgcccgcgcc gcgacgatcc ggtcgatcag
     1201 cgggcgaagc gaaacggtga cggaatcggt ggtcgtgctg tcgagcacga atctcacgac
     1261 cacggaatcg gcggccgtct cgattgcgcc ggcgaccggc tcgccggccg gactcggcgc
     1321 agccgcccgc ccgccgtagc cggcccgcag catgtggtcg tatccggcga ccgaaatcgg
     1381 agtctgcgga tcaatgcgga ccgcgaagta ctgctcccgc ccgggcgtat cgtatggatg
     1441 gaactcgatg cccagaagtt cggcgacgta ctccggcccc ttgaaccgat tcccggcatc
     1501 ctccgtgtcc gcgcggaggg tctcgccgaa ccacggctcc agggcggaga agccgtgcac
     1561 ctcgtacaag tagatgagaa tcgcgctgat ctggacctcg tgatcggcgg gaaccggggc
     1621 cggcgccttt gtcaccgcgc cgttcaccag gatcgaatcg gcagtcagca tcccacggag
     1681 tctccggatc tggctctgct ccgagacgct gaacattccc cagggaccga ctgagatcaa
     1741 aaaggcgaag gcgcagaggg aagcggggat catcctgatg ctcttgcaac ggctgagaag
     1801 gaagtacgcg gcgatgacgg ccagccagac gcccacggcc aggcccagat acctcccctc
     1861 ggtcagaccg tactcggaga tgcgccgcca gagcgccagg aagaggacga cgatgagggg
     1921 gaggaggatc aggtagaacc aacgggacgc tctctttatc cacagggact ccgtcctctc
     1981 gcgcatggga tcgagcaccg agagggcgag gatgccggtc gcggagaagc cgatgatcag
     2041 ccgccccacc cagccctgcg gccagctcca ggtgacgatg atcttggcga tgtaggcgta
     2101 gaggatcacg aagtagaccg cgacgagagg cgggagaaca tactgcccca gcaccttcag
     2161 cggccgggga taatcgctcc ccgtcccgag cgcgctccaa tcctcgggca gtccggccaa
     2221 aacatagggg acggcaaaga gcccgagggt aaggatccag agttccgcat accgcttcga
     2281 cggtatcgcc atgccgaaga gattgtccag cgcggccagc gccagcgcca ggccggcgaa
     2341 gaggaccagc gcgaacgccc ccgcgagcat gattcggaag acgaccaggc tggtgaaatg
     2401 ccagaagcgg ttgtcctccc ctttctgccg gaacggccac accgaggcca ggagcgtgca
     2461 cccgatcccc agcgcaaaga aacgcaggag atgcaccaga ggtgcgcggg ggaggtcggt
     2521 cggaacggtg gcggcgtaca ccaccagcag ggcggcggcc gccgcctgag ccggccaggc
     2581 gctgctcgcg ggtgcgctgc tcctctctgc ggccagcttc acggccgtca tcacgggaag
     2641 ggcaaggatg gcggtcagga ggatgttgta gagaaccgac gcctcgcggc tgccctcctg
     2701 atccacaaga atcaaggccg ccagggttcc cagcagcgcg cagaccagca caaggggaaa
     2761 acgccgaatc gtccggccat actcggccgc aagctgtgcc agcgacggca gtttcatatc
     2821 gattccttcc tcgagtaatt ctctgtctgc gggttttata cgaagaccgc agtggtacgg
     2881 caacttacaa cttttcggct gccgggcggg gggcgggtcg ggagccggac tcgaactcgg
     2941 cgataacaag aaaaaccggc ccacgagtgg gccggtttac ttttgcgggt cgagagccac
     3001 ggaatttgaa aggactcaca ccattacggt acggtaaagg gtttttacag tcacttcccg
     3061 cgtcttgctt tcgctaccgt ccaaaggacg ggtcacggcg tgtgcgcagc gacgaagaga
     3121 cgaccttcat tagtccagtg taagaggttt tcaaattaca tttctctctc cccgtcactt
     3181 gtatggtacg tcccatcggc gccggaagca attagttttt tgactctgtt gcgggaaatc
     3241 gcgccgctgc ctatcttccc tcgccggcct cagccgcgga aaagagtatg attcaggcaa
     3301 tcggactgga cattgtggaa gtcgcgcgga tggaacggga tctccgaacc tggggcaacc
     3361 gtttcgccga acgcatcttg ggggcggagg aactcgccat cctcaatcgt cgacacgaca
     3421 agtctgtgtt tttggccggg cgcttcgccg cgaaagaggc ggtcatcaaa gccctcggct
     3481 accggctgag cgagcgcccg gcctaccgcg cgatcgcaat caccaacgac gcctccggac
     3541 agccggtgct ctcgctgccc gatgacatcc gggcggccct gggccgcgcg cggtgtttcc
     3601 tgtcaatcac acacgagcga acgcatgccg ccgccgtggc ggtcataacg gaggattcat
     3661 gagccaggat gacgtggtga acctgttcaa ggagtccggg gcgcttctga ccgggcattt
     3721 caagctgacc tcgggacggc actcggacgt gtactatgaa aagttcacgc tgctcaaaca
     3781 acccgcgctc tgcaccaggc tgtgcgcgcg catggccgac acgctccgta ccacgggtgt
     3841 ccggacgatt gtcgggccga cgaccggggg gatcatcctg gcctacgatg tcgcgcggta
     3901 cctcggcgtg gaagcgatct acgcggaggc ggcggacgac ggcaaggggc gggtgttcaa
     3961 gcgggggttt cacctcgagc cggggcagaa ggtggcgatt gtcgatgacg tgctgacgac
     4021 cggaacgtcg gtggacgagg tgatcgccct ggtccgccgg tacggcgccg agatcgtcgg
     4081 gctgcggctc atgctcgatc ggtcgaacgg gaaggtcagg tttgactatc cgttcgccgc
     4141 gctggcgacc gtggcggccg agagctggga tccggcggag tgccccctgt gcgcgaaagc
     4201 gatgccgctg acccagcggg gcagccggaa gttctgaccg cgccgcgccg ggcccgacga
     4261 ggccgcgctc cgcgggcggc cggaccgccc tacttgtggt agtcggtccc gtggagaatg
     4321 gagaaggctc ggtagagctg ctccagcagg atgagccgca cgagctggtg cgaaaaagtg
     4381 agcggggaga gggagagaac cgtgtcggcg cgagagagca ggtcccggtc gagaccgtgg
     4441 ggaccgccga tgacgaaagt gaggttgctc cccgacaccg tgtgccagcg ctcgaggagc
     4501 cgggcgaaag ccgggctgtc gaccgtccgg ccggaggccg cgagggccgc gatgtacccg
     4561 ggaccgagcg ccgcccgcag gcgcgccgac tccgcccggc ggatctcctc gggagagagc
     4621 gcggccgaat gcttcggcga cggcacgatg cgccaggcga gacggcacca gcgggcaaga
     4681 agtttctcgt agtgcgcgac cgcctccgat acccagcggt ccttgtccct gccgacggca
     4741 agaacggtga tgttaagcac cggacatcac tctgacgata aggtggcggc ggcgggggcc
     4801 gtcgaactcg cagaagtaga ttccctgcca ggcgccgagg accaggtcgc cgccctcgat
     4861 cagcacgtgc gccgagaccc cgaccagcgt gctcttgacg tgggcgtcgg aattcccctc
     4921 ggcgtggcgg tagccgtcgg ccgccgggat ctccttgtcc agcttgtaga gaatgtcgcg
     4981 ggccacgtcg gggtcggcgt tctcgttgat ggtgaccgcc gcggtcgtgt gcggcacgaa
     5041 gcagacggcg atgcccgagg cgacgcccga ttcccggacg gcgcggcgga cctgcgcggt
     5101 gacgtcgatc atctcctcgc gcgcgtgcgt ctcaacgctt agccggctcg tcggcaaaga
     5161 gggcctccgt gtactcgcgc gggttgaacc gctggatgtc gtcgatcccc tcgccgaggc
     5221 cgatgaaatc gacggggacg ttgagttcct gggcgacggc gatcatcacg cctcctttgg
     5281 cggtgccgtc gagcttggtg acaatgaggc cgtcgcagcc gaccgcgtcg gtaaagaccc
     5341 tgacctgcga cagggcgttt tggccggtgg aaccgtcgag gatgaggcgg gtggagatcg
     5401 cggcctcggg ggcggccttg gcgatcacgc gccggatctt cttcagctcc tccatcaggt
     5461 tggacttcgt gtgcagacgg ccggcggtgt cgacaagcag ga
//
LOCUS       DAOOKS010000024         5483 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_17850_length_5483_cov_3.506080, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000024 DAOOKS010000000
VERSION     DAOOKS010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5483
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17850_length_5483_cov_3.506080"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="PK112_00810"
     CDS             complement(<1..231)
                     /locus_tag="PK112_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10624.1"
                     /translation="MGNLLSIIIQLICGAVGGNVAGSLMKKSSLGTIGNSIAGILGGG
                     IGGQILGALGVGAAGGGTDVGSIVGSILGGGVG"
     gene            complement(467..1912)
                     /locus_tag="PK112_00815"
     CDS             complement(467..1912)
                     /locus_tag="PK112_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HPC10625.1"
                     /translation="MDALLIAKAGRDLHLLPRMANRHGMIAGATGTGKTVTLQVMAEQ
                     FSRLGVPVFLADVKGDLAGLSQPGGGNARVDERLKALGVNDWIWQGCPVRFWDVYGEQ
                     GHPVRTTVSDMGPLLLSRLLDLNDTQAGVLSLVFRLADDNGLLLVDMKDLRAMLQYAA
                     DNSRRIQTAYGNISPASVGAIQRALLALEEQGGDRLLGEPALDIDDLRQTDGDGRGVV
                     NILAADRLLQTPRVYTTFLLWLLSELFEQLPEAGDLDKPKLVFFFDEAHLLFDEAPKV
                     LVEKIEQVVRLIRSRGVGVYFISQNPLDVPDDVLGQLGNRVQHALRAFTPKDQKAVRS
                     AAETFRANPALDVERAITELATGEALVSLLDAQGSPSPVERALIYPPHSRIGAIAPET
                     RRDLIGQSPLRGRYETLLDRESAYEMLRARADRAAADGASPSPRPGRPSSGASGNAAK
                     IATSIARSAGSQLGRELVRGVLGSLFGRRRR"
     gene            2127..2816
                     /locus_tag="PK112_00820"
     CDS             2127..2816
                     /locus_tag="PK112_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10626.1"
                     /translation="MKLKKGLLALGVVILAAATGSAQEPARSGAVDSRMVVIPMRSVA
                     EVTADIDNATMVKEMAAAQKAQAEGRLKEIELTIEQKQTALKDVDRRKGEAKKGQRQS
                     EVIALDIEKKVEQQAIDLLKRLKDLRKAEIEQAQVEGELAERRIGVLQLEAELQRKRI
                     EYDSLSTAGASPLSQTAAQQVLGEIEVKLLKLQQEQAGVTQKLASRQKDIVARRIKLH
                     DAQVKLGMPRA"
     gene            complement(2932..4176)
                     /locus_tag="PK112_00825"
     CDS             complement(2932..4176)
                     /locus_tag="PK112_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10627.1"
                     /translation="MDTLRLTARWALPALAVIVAGCSKSVDVEDEETDGTPPLAVANL
                     AVMASTSQSAVLRWTTPADRRDDQSPGAIAAYDLRFSFDSITDENFAAAVPVEPVPAP
                     LPAGHVQQWTVFGLTPDSGHYFALKSRDRRGNWSAVSNCPWVHCAAVRPAAFADGALE
                     QAVRSHIHKPSGDILTSDIDTIGQLVVRDAGIASLAGLEDCRSLAAVDFARNDITSLA
                     PLSGLPLLSGLYLAENNVSDLNPLAGMASLRQIQLQDNPVSDLGPLATIDSLQQLILW
                     GTPVTDFSALYELRFLSDVGFDRMNLADISFMSHLKRPQICGLAFNNITSLEPLRPLY
                     MLESLNLMQNHITDLAGLTPLTGLRELNLANNQIVDLLPLIYNTGLDSGDVIRLEGNP
                     LSDYARAIQIPTLRLRKVTVIF"
     gene            complement(4247..>5483)
                     /locus_tag="PK112_00830"
     CDS             complement(4247..>5483)
                     /locus_tag="PK112_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HPC10628.1"
                     /translation="AVRTLPKDALLQDEPDERLEREPIFLGLIGMIDPPRAEAQEAVA
                     RAVGAGMRPIMITGDHPRTAAVIARELGIACDGRAVTGAEIESTEDAAFDRLVQEVSV
                     YARVNPEHKLRIVRALQRGGATVAMTGDGVNDAPALKTADIGVAMGITGTDVSKEAAD
                     MVLADDNFATIVAAVEEGRAIFSNIRKFLRYLLSSNIGEVMTMFLGVVGADLLGLRSD
                     GGGAIVLPLLATQILWVNLVTDGAPALALGVDPASRGLMNRPPRPRRERVITRRMWSG
                     ILFVGAVIAVGTLFAVDACLPGGLVEGAGSLRYAQTMAFTTLVLFSLFNVFNARSDER
                     SAFRGLFANRWLWGSVLLSLALQAAVIYLPFLQQTFSTVPLSLGDWLFCAAVASSVLW
                     LRELTKAAVRGRRRPANRP"
BASE COUNT          995 a         1641 c         2008 g          839 t
ORIGIN      
        1 gccgacaccg ccgccgagga tgctgccgac aatgctcccg acatccgtcc cgccccctgc
       61 cgcgccgacg ccgagcgcgc cgagaatctg gccgccgatg ccgccgccca gaatgccggc
      121 gatggaattt ccgatcgtgc ccaacgaaga ttttttcatc agcgacccgg cgacgttgcc
      181 gccgacggcg ccgcagatga gctggataat gattgataag aggtttccca tgtcgttgct
      241 cctttcactt cgaccacagt tggggctcgg gcagtgagtg catctgtgct ccactccggc
      301 acagacgcgg taaatgaaag tcgcccctac tgaataatga acccacctgt cgatgatggg
      361 gcaagttaaa agattggacg cgtaccgcga agtgcggttc tccggctgag cgggcggcga
      421 ggagggcgcg ccccggctac ggagggcgga gtccacccgc gctctactag cgtcggcggc
      481 ggccgaagag cgacccgagc accccgcgca ccaattcgcg accgagctgg ctgccggccg
      541 agcgcgcgat ggaagtggcg attttggcgg cgttgcccga cgccccggac gacggccggc
      601 ccggccgcgg ggagggagag gcgccgtccg cggccgcccg atcggcgcgc gctctgagca
      661 tttcgtaagc agactcgcgg tcgaggaggg tctcgtagcg gccccgcagc ggcgattgcc
      721 cgatgaggtc gcggcgggtc tccggcgcga tggcgccgat gcggctgtgg ggcggataga
      781 tcagcgcccg ctcgaccggc gatgggctgc cctgggcgtc cagcagcgac acgagcgcct
      841 cgcccgtggc cagttcggtg atcgctcgct ctacatccag ggccgggttg gcgcggaaag
      901 tctcggcggc cgagcgcacc gctttctggt ctttgggggt gaaggcgcgc agcgcgtgct
      961 ggacccggtt gccgagctgg ccgaggacgt cgtccggcac atccagcggg ttctggctga
     1021 tgaagtagac gcccaccccc cgggagcgga tgagccgcac gacctgctcg attttctcca
     1081 ccagcacttt gggcgcttcg tcgaagagca agtgcgcctc gtcgaagaag aagaccagtt
     1141 tcggcttgtc caggtcgccg gcctcgggga gctgctcgaa caactcggag agcagccaaa
     1201 ggagaaaggt ggtatagacg cggggggtct gcaggagccg gtcggcggcg aggatgttga
     1261 ccaccccgcg gccgtcgccg tcggtctgcc ggagatcatc gatgtcgagc gccggttcgc
     1321 ccagaagccg gtcgcctccc tgctcctcca gcgcgagcag agcgcgctga atggcgccga
     1381 ccgaggcggg ggagatgttg ccgtacgcag tctgaatgcg ccggctattg tcggcggcgt
     1441 actggagcat ggcgcggaga tccttcatgt ccacgagcag caggccgttg tcgtcggcga
     1501 ggcgaaacac gaggctgagc accccggcct gggtgtcgtt gaggtcgagg aggcggctca
     1561 gcaacagagg gcccatgtcg gagacggtag tgcggacggg atggccctgc tcgccgtaga
     1621 catcccagaa tcgcaccggg caaccctgcc agatccagtc gttgacgccg agcgccttca
     1681 ggcgttcgtc gacccgggcg ttgcctccgc cgggctgact gagaccggcg aggtccccct
     1741 tgacatcggc gagaaaaacg ggcacgccga ggcggctgaa ctgctcggcc atgacttgga
     1801 gggtgacggt cttgccggtc ccggtggcgc ccgcgatcat gccgtggcga ttcgccatgc
     1861 gcggcaacag gtgcagatcg cggccggctt tcgcaatcaa aagagcatcc atgcgtacct
     1921 ccttcgcctc gcacggcagt atacaatgct gccggggatc gacaaagaaa aatcccccgc
     1981 tgacagccgc cgtcggacca ggccgtccgc gaagacagga attcggtata aaggccgttg
     2041 acaccggcgg cgcgggcctt atgttacggc ggccccggag gtatgttctc aaacgggccg
     2101 gtcgaggccg gccggaggtt gtttacatga agctgaagaa gggacttctc gcactgggtg
     2161 tggtgatcct ggcggcggcc accggctccg cgcaggagcc ggctcggtcg ggcgcagtgg
     2221 attcgcgcat ggtcgtcatt cccatgcgca gtgtggcgga agtgacggcc gacatcgaca
     2281 acgccacgat ggtgaaggaa atggcggccg cccagaaggc gcaggccgaa gggcgtctga
     2341 aagagatcga gctcacgatc gagcagaaac agacggcgct caaggacgtc gaccggcgca
     2401 agggagaggc caagaagggg cagcggcagt ccgaggtgat tgccctcgat atcgagaaaa
     2461 aagtcgaaca gcaggcgatc gacctgctca agcggctgaa ggatttgcgg aaggcggaga
     2521 tcgagcaggc gcaggtggag ggggagttgg cggagcgccg tatcggcgtc ctccaactgg
     2581 aggccgagct ccagcggaaa cggatcgagt acgattcgct gagcacggca ggcgccagcc
     2641 cgctttcgca gacggcggcg caacaggtgc tcggagaaat tgaagtgaag ctgctgaagc
     2701 tgcagcagga acaggcgggc gtgacgcaga agctggcttc ccgccagaaa gacattgtcg
     2761 cgcggcggat caagctgcac gatgcgcagg tgaagctcgg catgccgcgg gcgtgacctt
     2821 ggccgcaagg gcggtcggta tgacaatgat gccggccggg ctgcagcccg gccggtattt
     2881 ttttggagtg tttgccggcc gcgatccgct ccgaaaggcc gaccgcacga ttcagaaaat
     2941 gacagtcact ttccgcaacc ggagggtcgg tatctggatc gctcgcgcat agtcggagag
     3001 cgggtttccc tccagccgga tgacgtcgcc cgaatcaagg ccggtgttgt agatcagggg
     3061 caacagatca acgatctggt tattggcgag attgagttcc cgcaggccgg tcaggggcgt
     3121 caacccggcg agatcggtga tgtgattctg catgaggttc agcgactcga gcatgtacag
     3181 cgggcggagc ggttcgaggg aggtgatgtt gttgaaagcg agcccgcaga tctgcggccg
     3241 cttcaggtgg ctcatgaagc tgatgtcggc aaggttcatg cggtcgaagc cgacgtcgct
     3301 gagaaaacgg agttcgtaga gagcggagaa atcagtcacc ggggtccccc agaggatgag
     3361 ctgctggagg gagtcaatgg tcgccagcgg gcccaggtcg ctgacggggt tatcctgaag
     3421 ctgaatctgg cggaggcttg ccatacccgc cagggggttg agatcgctga cgttgttttc
     3481 ggcgagatag aggccgctca gcagaggaag gccggagagc ggcgccaggc tggtgatatc
     3541 attgcgggcg aagtccacgg ccgccagaga ccggcagtcc tccagacccg cgagcgaggc
     3601 gatgccggcg tcccggacga cgagctggcc gatggtatcg atgtcggacg tcaggatatc
     3661 gccggaaggt ttgtggatgt gggagcggac cgcctgctcg agcgcgccat cggcgaaggc
     3721 cgccgggcgg acagcggcgc agtgcaccca cgggcagttg gacacggcgc tccagttgcc
     3781 gcggcggtcg cgcgacttca gggcgaaata atggccgctg tcgggggtca ggccgaagac
     3841 agtccactgc tgaacgtgtc cggccgggag cggcgccggc accggctcga ccggaaccgc
     3901 ggccgcgaag ttctcgtcgg tgatgctgtc gaaagagaag cggagatcgt acgcggcgat
     3961 agcgccgggc gactgatcgt cgcggcggtc cgccggcgtc gtccagcgga gcacggcgct
     4021 ctgggaggtc gacgccatca cggccaaatt ggccacggcg agcggcggcg tgccgtctgt
     4081 ttcctcgtct tcaacgtcga cagatttgct gcagccggcg acgatcacgg cgagcgccgg
     4141 caacgcccag cgggcggtta agcgaagggt gtccatcagc atggttccgg tgtccgaaca
     4201 aagagtgatt tgtctccgtc cagagtaggg ccgggcggac gcggtgtcaa gggcgattcg
     4261 caggccgccg gcggccgcgc acggcagcct tggtcagttc gcgcagccac agcacggaac
     4321 tggcgaccgc ggcgcagaag agccagtcgc ccagcgacaa cggcacggtc gagaaggtct
     4381 gctgcagaaa aggcaggtag ataacggctg cctgcagggc gagcgacagc aggaccgacc
     4441 cccagagcca gcggttggca aacagcccgc ggaaggcgct gcgctcgtcc gaccgggcgt
     4501 tgaagacgtt gaacagcgag aagaggacga gggtggtgaa ggccatggtc tgagcgtagc
     4561 ggagactgcc ggcgccttcg accagcccgc cggggaggca ggcgtcgacg gcgaagagcg
     4621 tgccgacggc gatgacggcg ccaacgaaga ggatccctga ccacatgcgg cgggtgatga
     4681 cccgctcgcg gcgggggcgg ggcggacggt tcatgaggcc gcggctcgcc ggatcgacgc
     4741 cgagggcgag agcgggcgcg ccgtcggtaa ccaggttgac ccacaggatc tgcgtggcca
     4801 gcaggggcag cacgatcgcg ccgccgccgt ccgacctgag gccgaggagg tcggcgccca
     4861 ccacgccgag gaacatggtc atgacttcgc cgatattcga cgacaggagg tagcggagga
     4921 atttgcggat gttggagaag atggcgcggc cctcctcgac cgcggcgacg atggtggcga
     4981 agttgtcgtc ggccagcacc atgtcggcgg cttctttcga gacgtcggtg ccggtaatgc
     5041 ccatggcgac gccgatgtcg gcggttttga gagcgggggc atcgttgacg ccgtcgccgg
     5101 tcatggccac ggtggcgccg ccgcgctgga gggcccggac aatgcgcagc ttgtgttcgg
     5161 ggttgacgcg ggcatagacg gagacttcct gaacgaggcg gtcgaaagcc gcgtcctcgg
     5221 tgctctcgat ttcggcgccc gtgacggcgc ggccgtcgca cgcgatgccg agctcgcggg
     5281 cgatcacggc ggcggttctg gggtggtcgc cggtgatcat gatggggcgc atgccggcgc
     5341 ccacggcccg cgcgaccgcc tcctgcgcct ccgcgcgcgg cgggtcgatc atcccgatga
     5401 ggccgaggaa aatgggttcc cgctccaggc gctcatcggg ctcgtcctgc aggagggcgt
     5461 ccttgggcag cgtccgcacg gcc
//
LOCUS       DAOOKS010000025         5445 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_18028_length_5445_cov_4.146197, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000025 DAOOKS010000000
VERSION     DAOOKS010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5445
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18028_length_5445_cov_4.146197"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="PK112_00835"
     CDS             complement(<1..689)
                     /locus_tag="PK112_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10629.1"
                     /translation="MTPDHSDGGAGGGAGGSGAASGWTPPIVRPAEREDTPPPAAAPD
                     DDAPAAESDMSAAAGSPPAPTAGKSDSPTVSATGLVTGNKYAICVRDSYLNHLEAEFD
                     LKAGDIDDEGVRRLKRRGKKRKQTTPDGWVVELQDHLKKFVIGDVGESDGIFGRKGVL
                     KAVQAFQAAALTKHRWQGKELKEVQITYKGKIDGLVDRDVKEEITRWLGNGYTAYYPK
                     LTASVGRRKNK"
     gene            complement(764..2977)
                     /locus_tag="PK112_00840"
     CDS             complement(764..2977)
                     /locus_tag="PK112_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10630.1"
                     /translation="MTALKSTKATDAEHEVKLEPRLISLSWRSGKARAGGIARFEVVT
                     EFVGNGAKVKVTGKSEKGKKLGAVDALMRRNRLTGQFDVPADIDLDDRVFFEVRLPGN
                     DLEGTSDLIPAAPPVKVNSLRWSCDEARRDDEVALSAFVDGVDEAEEVALTIYEFDRD
                     GAHDKITELPGQVVDGRVEKKWRFEYHEDVDEIPTQEEMERYGGAYNPPEYFFTITVD
                     GVEFGKKRASPHLLTFRDWIELSLCDSSGRPLANEDYELLLADGTKRTGTLDGEGKAK
                     VDGVPPGRFQLKLANVRGSLAHGRPAVQQTCPKARAIAKVEGPSRIRVGGRAEYTAKR
                     AQNADQSAPVKWRVSDIESEEVLEVETEIQQDTLILPCIPDSFKGKTVKVEAWQEEPE
                     DGATFSSLVRYDEVGEYIARCRKLENAFPDWTGEAVLNALRRLAGCDDKRFQRMFGVA
                     AADNLQEMCDSRRLPSGVAADDIKWLIEHSRHREKIDPPDDRSGAMKLKEQPQQKLEA
                     DLRGCATDCLGYPLAMGHVLCGISAGQHRNTSVRPHFLVVPGALGWHVDNLYAVTVAG
                     DLGQSAVCVFLGDKSVLIGDDRATTEATYDELRGDVDGFVLGHHLKSLLNGRSLDKLP
                     SDGGLRLSELLQTYYCVCEATASSSLPTAATRFAAFKRAGRHHLLDEIRQFALTFGYS
                     KPIFQKLRALLVQKNFDESGEKQPEPVKHMKALIQRIYDEFLAWLDKHADAEAKR"
     gene            complement(2992..3468)
                     /locus_tag="PK112_00845"
     CDS             complement(2992..3468)
                     /locus_tag="PK112_00845"
                     /inference="COORDINATES: protein motif:HMM:NF020366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HPC10631.1"
                     /translation="MVADPLCEGWPVIDEMGKTNKRFSMRLAEGWEDQTVHLFVGPAE
                     RGMNHHLTIAVDDIPLAETAQDYAEYRVEQFLDTLPAAEVLKEEPLALGEDLDAYEVV
                     IKFVPSEAGPRFRRLVFVLHENTAFTFAADFTKVTRNTLGAQMIEMIKTFRPEAGA"
     gene            complement(4307..4576)
                     /locus_tag="PK112_00850"
                     /pseudo
     CDS             complement(4307..4576)
                     /locus_tag="PK112_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015834410.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
     gene            4925..>5445
                     /locus_tag="PK112_00855"
     CDS             4925..>5445
                     /locus_tag="PK112_00855"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HPC10632.1"
                     /translation="MNEQLIGDYRVIEERGAGGMARVYLAVHREVPSLKVILKVLKDP
                     QHAERFRQEADKLALLEEDSRVCRIKGFFQWEGDLIIVMEFIDGVTVEDLIKSSGHLP
                     VREAVLIAVETLDVLAFAHRKGIYHRDIKPSNIMRDKNGAVRIIDLGIAKAKTDPSLT
                     AVGGCCGTPEYMAP"
BASE COUNT          819 a         1791 c         1760 g         1075 t
ORIGIN      
        1 ttcttgttct tgcggcggcc gacgctggcg gtgagttttg gataataggc ggtgtaaccg
       61 ttgcccagcc agcgagtaat ctcctctttc acatccctgt cgaccaggcc gtcgatcttc
      121 cccttgtagg tgatctgcac ctctttgagc tctttcccct gccagcggtg cttggtcagc
      181 gcggcagcct gaaacgcctg caccgccttc agcaccccct tcctcccgaa gattccgtcg
      241 ctctccccga cgtcgccgat aacgaacttc ttgaggtggt cctgaagctc gaccacccat
      301 ccatcggggg tggtctgttt gcgcttcttt ccccggcgct tgagccgccg caccccctcg
      361 tcgtcgatgt cgccggcttt gagatcgaat tcggcttcca ggtgattgag ataggaatcg
      421 cggacacaga tggcgtattt gtttccggtc accagtccgg tcgcggagac ggtgggcgag
      481 tcgctttttc ccgcggtggg ggccggcggg ctgccggcgg cggccgacat gtccgactcg
      541 gccgcgggag cgtcgtcatc gggcgcagcg gcgggcgggg gggtatcttc gcgttcggcc
      601 ggtcggacaa tcggcggggt ccagccgctt gccgccccac tgccgccggc gccgccgccg
      661 gccccaccat cgctatggtc aggcgtcata tccgctctcc gcgttcccgc actccgtcca
      721 atcgtttttg ctgcagttct tcttcaccca tccttcggcg acgctatctc ttcgcttccg
      781 cgtcagcatg tttgtccagc cacgcgagga attcatcgta gatgcgctga atgagcgcct
      841 tcatgtgctt taccggttcc ggctgtttct ccccgctctc gtcgaaattt ttttgcacga
      901 gcagggcgcg cagcttctgg aagattggct tgctgtaacc gaacgtgagc gcgaactgcc
      961 ggatttcatc gaggagatga tgccggcccg cgcgcttgaa tgccgcgaac cgcgtcgccg
     1021 ctgtcggcag gctgctcgat gccgtggcct cacagacgca gtagtaagtc tggaggagtt
     1081 cgctaagacg gagtccgccg tcgctgggca gtttgtccag tgaacgtccg ttcagtagcg
     1141 acttcaagtg gtggccgagg acaaaaccgt cgacatcgcc gcgcaactca tcgtaggtgg
     1201 cctcggtcgt ggcccgatcg tcgccgatca aaacactctt atcacccagg aagacgcaca
     1261 ccgccgattg gccgaggtcg ccggctacgg tgacggcgta caggttgtcc acgtgccatc
     1321 cgagcgcgcc cggaacgaca aggaaatgtg gccgcacgga ggtattccga tgctggccgg
     1381 ccgagatgcc gcacagcacg tggcccatcg ccagcgggta tccgaggcag tcggtggcgc
     1441 agccgcgcaa gtctgcttcc agtttctgtt ggggctgttc tttcagcttc attgctcctg
     1501 accggtcatc aggagggtcg atcttctctc ggtgtcggga gtgttctatc agccacttga
     1561 tgtcgtcggc ggcaaccccg gagggcagtc gtctggaatc gcacatttcc tgcaggttat
     1621 cggccgccgc caccccgaac atccgctgaa accgtttatc gtcgcacccc gccagccgcc
     1681 gcagggcgtt gaggaccgcc tcccccgtcc agtccggaaa ggcgttctcg agtttgcgac
     1741 agcgggcgat gtactccccc acttcgtcgt acctgacgag cgaggagaaa gtcgcgccgt
     1801 cctccggctc ctcctgccag gcctcgacct tgaccgtttt ccccttgaac gagtccggga
     1861 tacacggcag gatcagcgtg tcttgctgga tttcggtttc gacctccaga acctcctccg
     1921 actcgatatc gctgacgcgc cacttgaccg gcgccgattg gtcggcgttt tgcgcacgct
     1981 tggccgtgta ctccgcccgt ccgccgacgc ggatgcgcga gggtccttcc actttggcga
     2041 tcgcccgggc tttcgggcaa gtctgctgga ccgccggacg gccgtgggca aggctgccgc
     2101 gaacgttggc cagcttgagc tgaaaccggc cgggcggcac gccgtcgacc ttcgccttgc
     2161 cctccccatc gagcgtcccc gtccgcttgg tcccgtcggc gaggagcagt tcgtaatcct
     2221 cgttggccag cggccggccg gaggagtcgc acagactcag ctcgatccag tcgcggaaag
     2281 tcagcaggtg gggggaggcg cgcttcttgc cgaactcgac gccatcgacg gtgatcgtga
     2341 agaagtactc gggcgggttg taggcgccgc cgtaccgctc catctcctcc tgggtgggga
     2401 tctcgtcgac atcttcgtgg tactcgaacc gccacttctt ctccacccgg ccgtcgacca
     2461 cctggccggg gagttcggtg attttgtcgt gcgctccgtc gcggtcgaac tcgtagatcg
     2521 tcagcgccac ttcttccgcc tcatcgaccc cgtcgacaaa tgcggacagc gcgacctcat
     2581 cgtcccggcg ggcttcgtcg cagctccacc ggaggctgtt gactttgacc gggggggctg
     2641 ccgggatcag gtcggaggtc ccctcgagat cgttgccggg gagcctgacc tcgaagaaca
     2701 cgcggtcgtc gaggtcgata tcggcgggga cgtcgaattg tccggtgagg cggttcctgc
     2761 gcatgagggc gtcgacggcg cccagttttt ttcctttttc gcttttgccg gtgactttca
     2821 ctttcgcgcc gttgccgacg aactcggtca ccacctcgaa ccgggcgatt cctccggcgc
     2881 gcgcttttcc ggagcgccaa ctgagcgaga tgagcctcgg ttcgagcttg acttcgtgct
     2941 cggcgtcggt ggctttggtg cttttcaggg cggtcataca gcctcgcggg ttcaggcgcc
     3001 ggcctccggc cggaatgtct tgatcatctc gatcatctgc gccccgagcg tattccgggt
     3061 cactttggtg aaatccgccg cgaaggtgaa ggcggtgttc tcgtggagca cgaacacgag
     3121 gcggcggaac cggggtccgg cctccgaggg cacgaatttg atcacgacct cgtaggcgtc
     3181 gagatcctca ccgagcgcca gcggctcctc tttgagcacc tcggccgccg gcagggtgtc
     3241 cagaaactgc tcgacgcggt actcggcgta atcctgggcc gtctccgcca gggggatgtc
     3301 gtcgaccgcg atcgtcaggt ggtggttcat gccgcgctcg gcggggccga cgaacaggtg
     3361 cacggtctgg tcctcccagc cctcggcgag cctcatcgag aaccgcttgt tggttttccc
     3421 catttcatcg atcaccggcc atccctcgca taacgggtcc gcgaccatgt tgacgtcggc
     3481 aggcgagacc ggttgcagtc gcgggcaacc ggcgtggtca cgcctgcggt tcaaagaata
     3541 tctgcgcctt tcccggcggc acgttttcca accgggcttt gccgtcgctg tccagcgtcc
     3601 cctcgcggat ctctccgttg gggaagacga ctttgtaggc ttttccggcg gccgcttttc
     3661 cctggctgtc ggcaagccgg atttcgagcc agtcgctgta gaagaggaag ggcgagcggg
     3721 cagcgcagcc ctcggcctca gcgacaaagt agaaccgcgg actggaatag ccgctccggg
     3781 cctgctgcga tgactgcagc ctgaggatct tttcgtcgac agcgaagacc cagtccgcct
     3841 cgatggcatc gttggcgacc tcggccggaa gctcggccac cacgcggtcg gggacgttgt
     3901 agtcgcgcac gaatattttc agagcggcct tggttccgga ttcgagtccg gcgcacacgg
     3961 cgcgcagggt caccgtatcc cccaccttgg cccgctcgac cgaccactcg gccttggaga
     4021 tgcagcgcag gccgatctcg taacttccgg catccactcc gtcgcggcgg atgcggccgg
     4081 ccaggaggcc ggagtccgtc ttgccgtcgg ggcccttgac gacgtacccg acgccggtga
     4141 tcggtttgcc ggctttgtcc acgtacttga cgtcgaggaa gtgtccctcg ggttcttgct
     4201 cggcggcttc gccggcgccg ccgccttcgc cggccgcggt ctcggcggtt cccgaggcgg
     4261 cgccgctgcc ggcgccgccg ccgcctcctc cgccgccgcc gccggggccg atgagcacgg
     4321 tcgggcaacc ggggggtgca atcgcatcgg gcgggccgga acaggtggcc atgtcgccga
     4381 cacaggccgc cggtttgccg ccgatgagga cggtgacggc gccggggggc atgatggggc
     4441 cgcccacgtg gggcgggggc ggggcccccg ggttaaccat cgggcaggtg tgcatgtcgc
     4501 cgacgcgcgc ggccgggagg ccgccgatga ggacagtcgg ttcgccgacg acgatggctc
     4561 cgccgtggac cgtcatatca cccatccgcg ctgccggctt gcccatgctc agatctccct
     4621 gttcggaaag ggcggccgag gtcgcgatcg cctcgccgcc gctccgctgc gcccatggtg
     4681 ccggccgccc ggttcggcgg cgggcacatg gttttttcga tcgctgctgt ctacaataat
     4741 aaaggcgcgg gcgcgcggcc gtcaatcatt gttttgcgcc gtccgcggcg cggggggcat
     4801 cgaccgccgg cccggcgcag ccggggagcc gcggccgaat tcgccttgca ttccgccccg
     4861 ggcgtccgta atttgactcg ccatcaacga cgcggccgaa gggccggaac cggggcgcca
     4921 gcgaatgaac gagcaactga tcggcgacta cagagtaatc gaagagcgag gggccggcgg
     4981 gatggcccgc gtctacctcg ctgtccaccg ggaagtaccc agtctcaagg ttatcctcaa
     5041 agtcctgaaa gatccgcagc atgccgagcg cttccgccag gaagccgaca agttggcgct
     5101 gctggaggaa gactcccgcg tctgccggat caagggtttt tttcagtggg agggggacct
     5161 gatcatcgtg atggagttca tcgacggcgt cacggtcgag gacctcatca aatccagcgg
     5221 ccacctgccc gtgcgtgagg cggtcctcat cgccgtggag acgctcgatg tgctggcgtt
     5281 cgcgcaccgc aaggggatct accaccgcga catcaagccg agcaatatca tgcgggacaa
     5341 gaacggcgcg gtgcggatca tcgatctggg catcgccaag gccaagaccg atccgtcgtt
     5401 gaccgcggtg ggcggctgct gcggtacgcc cgagtacatg gcccc
//
LOCUS       DAOOKS010000026         5445 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_18029_length_5445_cov_3.578664, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000026 DAOOKS010000000
VERSION     DAOOKS010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5445
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18029_length_5445_cov_3.578664"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1227)
                     /locus_tag="PK112_00860"
     CDS             complement(<1..1227)
                     /locus_tag="PK112_00860"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HPC10633.1"
                     /translation="MMKFLMAGFSAALVAGCAVGPNYVRPEIETPGRWQNPDSVFAAD
                     TAAVRSETAWWGLFGDTVLTNLIAAALQENADVRIAAARVEEVMGRYGVARSDFFPKL
                     GAAGAASRGQLLSDEAGRGLESTRNRFQVNLAADWEIDLWGKLRRATEAARADLLATE
                     EARRGVVLTTVALVASTYVDLLSMDWQLEIARQTALTRREALDLFEVRREKGDLSDLI
                     FSQAEAEYWLAMSQIPLIEENIAFLENMISCLVGRNPGPVARGKTLEDLAPPAVPVGL
                     PSELLERRPDIRFAEEQLRAANARIGVARALYFPSLSLSGLLGYASDDLSDLVNSDTR
                     TWRVGGDVLAPIFRFGEIKGQVKAAEGRQKQALYAYVEAVHNAFRDTENALVRRRCTN
                     EQLQAEGKRVAALATYA"
     gene            complement(1224..4412)
                     /locus_tag="PK112_00865"
     CDS             complement(1224..4412)
                     /locus_tag="PK112_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HPC10634.1"
                     /translation="MSKFFVNRPIVAMVIAIVMVLVGLIVMRGLPISQFPDIVPPMIN
                     IATTYVGASAIDVEQSVATPLELQVNGVEDMIYMKSINTSDGMMTLQVSFEVGADLDV
                     ANMLTQNRVSQAQASLPPAVKNYGVTTKKSLVFPLLLVALTSPEESYDNIFLSNYAAI
                     NINDRLSRINGVGDVKLFGGGDYAMRIWVRPDALARLGLTVSDLVRAVQEQNVISPAG
                     QLGAPPAPPGTEFTYTVRTQGRLFNEEEFGRIVLRTNPDGSQVRLRDVARIELGTLMY
                     GQTSRNNGKPAAVIGVYQLPGSNALAVEESVSKTMEELAQRFPPDLVHEITLNTTRAV
                     EEGINEIVHTLFEAVLLVILVVFLFLQNWRATLIPLIAVPVSLVATFMVFPLLGFSIN
                     TLSLLGLVLAIGIVVDDAIVVVEAVMHHIEEGLPPKEATLKAMEEVSGPVVAIALILT
                     AVFIPVALIGGITGQLYQQFAITIAISVLFSAFNALTLSPALAAKLLRPKGQGRRSPL
                     QRFYDWFNRGFGRFTGGYLSVTGILVRKVMRGVVFIGILLAATALLLKGVPTGFVPEE
                     DNGYFLITVQLPDAASLERTDAVCRKIEAILGETRGVANYTAVAGYSISSQAPGSNVG
                     TFFVSLEPWEERTTGEESSFGLVAALNQRFAREIPGAAVFAFGPPAIQGLGTGAGFTF
                     MLQDMTGGSPQELAAMAEQFAAAARQRPEIGRVATFYRAGVPQMYAEVDRDKALKLGV
                     SIADVNTTLGSLLGANYINDFNRFGRVYKVYLAAEGEYRNETWDLGLFYVRNKNGEMV
                     PLSTLVTMKPVSGPQYTNRFNLYRSAEMMGGPAAGYSSSQALAALEEVAADVLPAGWG
                     YDWANMSYQEKKAAGTGGVVFVMALVFVFLILAAQYESWSLPLSVLLATPFAIFGAMT
                     GLWVSRMVDTMYVNNVFAQIGFVTLIGLAAKNSILIVEFAKMQKEKGLPTVEAALAAA
                     KLRLRPILMTSFAFILGVVPLVLAAGAGAEARKVMGMAVFAGLLVATSLGIFLIPMLY
                     VVVQKYFVRERSPEPARRCDDGAGAAEKGAGV"
     gene            complement(4416..>5445)
                     /locus_tag="PK112_00870"
     CDS             complement(4416..>5445)
                     /locus_tag="PK112_00870"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPC10635.1"
                     /translation="DVPVSREWLGQTLGAVDIEIRARVDGWLESIHFREGSEVRKGDL
                     LYTIDPAELEQKVVQAKAKVAEAQTMFVRAESDANRYRPLAEAKAVSQRDLEAVLAEY
                     GARQGEVEAARAALRLAEIELGYATITAPINGLIGLSAARVGDLVGRPPSPVILNTVS
                     RVDSVRVRFSITENEYLELARRLQATPDGPPARERFPLRLILSDGTRYPHVGSVSVGQ
                     REIDPATGTLQLEALYPNPERLLRPGQFARVVAEVGMRKDAVVVPARAVTELQGQQML
                     FTVGADNKVAVRRVAVGPSAGPFTVIDRGLAAGEKVIVEGQQRVRPDMPVRPVEIPAD
                     SLAGGGGC"
BASE COUNT          956 a         1835 c         1804 g          850 t
ORIGIN      
        1 cgcgtaggtg gcgagggcgg cgacgcgttt accctccgcc tggagctgct cgttcgtgca
       61 gcggcgccgc acgagagcgt tttcggtgtc gcggaaagcg ttgtgcacgg cctcgacgta
      121 ggcatagagc gcctgtttct gccggccctc ggccgccttg acctgcccct tgatctcgcc
      181 gaatcgaaat atcggcgcga gaacatcgcc cccgacccgc cacgtgcgcg tgtcggaatt
      241 gaccaaatcg gagagatcgt cgctcgcgta accgagcagc cccgacagcg aaagggaggg
      301 gaagtagagc gcccgggcca cgccgatccg ggcgttggcg gcgcgcagct gctcctcggc
      361 gaagcggatg tcgggccggc gctcgaggag ttcggacggc aacccgacgg gcacggcggg
      421 cggcgcgaga tcctcgagcg tcttcccgcg cgccaccgga ccggggttgc ggccgaccag
      481 gcaactgatc atgttttcca ggaaggcgat gttctcctcg atgagcggga tctgcgacat
      541 ggcaagccag tactccgcct cggcctggct gaagatgaga tccgacagat cccccttttc
      601 gcgccgcacc tcgaacagat cgagcgcctc ccgccgggtg agggccgtct ggcgcgcaat
      661 ctcaagttgc caatccatcg agagcaggtc gacataggtg ctcgcgacga gggcgaccgt
      721 ggtcagcacc acgccgcggc gcgcctcttc ggtcgccagc aagtcggccc gcgccgcctc
      781 ggtggcccgg cggagcttgc cccacaaatc aatctcccag tcggccgcga gattcacctg
      841 aaagcggttg cgggtggact cgagaccgcg gccggcctcg tccgacaaga gctgcccgcg
      901 cgaggccgcg ccggcggctc cgagcttggg aaagaaatcc gaccgggcca cgccgtaccg
      961 gcccatgact tcctccaccc gggcggccgc gatccggaca tcggcgtttt cctgcagggc
     1021 ggcggcgatg agattggtca gaacggtgtc gccgaacagg ccccaccagg cggtctcaga
     1081 gcgcaccgcg gcggtgtcgg ccgcgaaaac ggagtcggga ttctgccacc gcccgggtgt
     1141 ctcgatctcc ggccggacat aattggggcc gaccgcgcat ccggccacga gggccgccga
     1201 gaacccggcc atgaggaatt tcatcatacg cctgctcctt tctctgcggc cccggcgccg
     1261 tcgtcgcaac gccgggcggg ctcgggggac ctctcgcgca cgaagtattt ttgcaccacg
     1321 acgtacagca tcgggatcag gaatatcccc aggctggtgg cgacgagcag gccggcgaag
     1381 accgccatcc ccatcacctt gcgcgcctcg gcgcccgccc ccgcggcgag cacgagcggt
     1441 acgacgccga gaatgaaggc gaaggaggtc atcagaatgg ggcgcaggcg gagtttggcg
     1501 gccgccaggg ccgcctcgac ggtcggcaga cccttctcct tctgcatctt cgcaaactcc
     1561 acgatcagaa tggagttctt cgccgccaaa ccgatcagcg tcacaaaacc gatctgcgcg
     1621 aagacgttgt tgacgtacat ggtatcgacc atccgcgaca cccagagccc ggtcatggcg
     1681 ccgaagatgg cgaagggcgt ggcgaggagc acgctcagcg gcagcgacca gctctcgtac
     1741 tgggcggcca ggatcaggaa gacgaagacg agggccatga cgaagacaac tccgcccgtg
     1801 ccggccgcct tcttctcctg gtaggacatg ttcgcccagt cgtaacccca gccggcgggg
     1861 aggacgtccg ccgccacctc ctcgagcgcg gccagtgcct ggctcgaact gtagccggcc
     1921 gccggaccgc ccatcatctc cgccgatcgg tacagattga agcggttggt gtactgggga
     1981 cccgaaaccg gtttcatggt caccagggtg ctcagcggca ccatctcgcc gttcttgttg
     2041 cggacataga aaagtccgag atcccacgtc tcgttgcggt actcgccctc ggcggccagg
     2101 tacaccttat agacccggcc gaaccggttg aagtcgttga tgtagtttgc cccgaggagc
     2161 gatcccaggg tggtgttgac gtcggcgatg ctcacgccca gtttgagcgc cttgtcgcga
     2221 tccacctcgg cgtacatctg cgggacgccg gcgcggtaga aggtcgccac gcggccgatt
     2281 tcggggcgct ggcgcgccgc cgccgcaaat tgttcggcca tggcggccag ttcctgcggc
     2341 gacccgccgg tcatgtcctg cagcatgaag gtgaacccgg cgccggtgcc gaggccctgg
     2401 atggccggcg gcccgaacgc gaacaccgcc gccccgggga tctcgcgggc aaagcgctgg
     2461 ttgagcgccg ccaccagacc aaacgaactc tcctccccgg tcgttctctc ctcccagggc
     2521 tcaagcgaaa cgaaaaacgt gcccacgttg gaaccgggcg cctgggaact gatgctgtaa
     2581 ccggccaccg ccgtgtagtt ggcgaccccc cgcgtctctc cgaggatcgc ctcgatcttc
     2641 cgacagacgg cgtcggtgcg ctccagcgac gcggcgtcgg ggagctggac ggtgatgagg
     2701 aagtagccgt tgtcctcctc gggcacgaaa ccggtgggaa cgcccttcag gaggagcgcc
     2761 gtggccgcca gcaggatgcc gatgaagacc acgccgcgca tcaccttgcg cacgaggatg
     2821 ccggtgacgg agaggtagcc gccggtgaac cggccgaaac cgcggttgaa ccagtcgtag
     2881 aaacgctgca gcggcgaccg ccgcccctgg cccttgggcc gcagcagctt cgccgccaga
     2941 gccggactca gggtcagagc gttgaaagcc gagaagagca ccgagatcgc gatcgtgatg
     3001 gcgaactgct ggtacagctg gccggtgatc ccgccgatga gggccaccgg aatgaacacc
     3061 gcggtcagga tgagggcgat agcaacaacc ggtcccgaca cctcctccat cgccttcagc
     3121 gtcgcctcct tcggcggcaa accctcctcg atgtggtgca tcacggcctc gacgaccacg
     3181 atagcgtcgt cgaccacgat gccgatcgcg agcaccagcc ccagcagcga cagcgtgttg
     3241 atggagaacc cgaggagggg aaacaccatg aaggtcgcca ccagggacac cggcacggcg
     3301 atcagcggaa tgagcgtggc ccgccagttc tgcaggaaga ggaacaccac caggatcacg
     3361 agcagcaccg cctcaaacag cgtgtggacg atctcgttga tcccctcctc cactgcccgc
     3421 gtggtgttga gcgtaatctc gtgcacgagg tcgggcggaa agcgctgcgc gagctcctcc
     3481 atcgtcttgc tcacgctctc ctcgaccgcg agggcgttgg aaccggggag ctggtacacg
     3541 ccgatgacgg cggccggctt gccgttgttg cggctcgtct ggccgtacat gagagtcccc
     3601 agttcgatcc gggcgacgtc gcgcaggcgc acctgagaac cgtcgggatt ggtgcggagc
     3661 acgatgcggc cgaactcctc ttcgttgaac agccggccct gggtgcgcac ggtgtaggtg
     3721 aactccgtcc ccggcggcgc cgggggagcg ccgagctggc cggccgggct gatgacgttc
     3781 tgttcctgaa cggcccgcac gaggtccgac acggtcagcc cgagccgcgc cagggcatcg
     3841 ggccggaccc aaatgcgcat cgcgtagtcg ccgccgccga aaagcttgac gtcgccgaca
     3901 ccgttgatgc ggctcagccg gtcgttgatg ttgatagccg catagttgct caagaagata
     3961 ttgtcgtagg actcctcggg cgaggtcagg gccaccagga gcagggggaa cacgagcgac
     4021 ttcttggtcg tgacgccata gttcttcacg gccgggggta gcgacgcctg ggcctgcgac
     4081 acccggttct gggtgagcat gttggcgacg tccaggtcgg cgccgacctc gaacgacacc
     4141 tgcagcgtca tcatgccgtc gctcgtgttg atagacttca tgtagatcat gtcctccacg
     4201 ccgttcacct gcagctcgag cggcgtggcc accgactgct cgacatcgat agccgaggcg
     4261 cccacgtaag tggtggcgat gttgatcatg ggcgggacga tgtcgggaaa ctgcgagatc
     4321 gggaggccgc gcatgacgat caagccgacg aggaccatga caatggcgat gaccatcgcg
     4381 acgatcgggc ggttgacgaa gaatttcgac ataggtcagc accctccgcc gcccgcgagg
     4441 gaatccgccg gtatctcgac cggccggacc ggcatatcgg gacggacgcg ctgctggccc
     4501 tcgacgatga ctttctcgcc cgccgccagc ccgcggtcga tcacggtgaa ggggccggcc
     4561 gagggcccga ccgcgacccg gcgcaccgcc accttgttgt ctgcgccgac cgtgaacaac
     4621 atttgctgcc cctggagttc ggtcacggcc cgcgccggca ccacgacagc gtccttgcgc
     4681 atcccgacct cggcgaccac ccgcgcaaac tggccgggcc gcaggaggcg ctccgggttg
     4741 ggatagagcg cctcaagctg cagcgtcccg gtggccggat cgatctcgcg ctgcccgacg
     4801 ctgacgctcc ccacatgggg gtagcgggtg ccgtcagaca ggatcaggcg cagggggaaa
     4861 cgctcgcgcg ccggcggacc gtcgggcgtg gcctgcagcc gccgagccaa ctccagatac
     4921 tcgttttccg taatggagaa gcggacgcgg accgaatcca cgcgggaaac ggtgttgagg
     4981 atgacggggc tgggcggacg gccgaccagg tcgccgaccc gcgcggccga caggccgatc
     5041 aggccgttga taggcgccgt gatcgtggca tacccgagct cgatctcggc caggcggaga
     5101 gccgcccggg ccgcctcaac ctccccttgc cgtgcaccgt attcggccag aaccgcctcc
     5161 aggtcccgct ggctcaccgc tttggcctcc gcaagaggac ggtagcggtt ggcgtcggat
     5221 tccgcccgca cgaacatcgt ctgcgcctcg gccaccttgg ctttcgcctg aaccaccttc
     5281 tgctccagtt cggccgggtc gatcgtgtag aggaggtccc ccttcctgac ctcgctgcct
     5341 tcgcggaagt gaatgctctc cagccacccg tcgacgcgcg cccggatttc gatgtcgaca
     5401 gctccgaggg tctggccgag ccactcgcgg ctgacgggca catct
//
LOCUS       DAOOKS010000027         5434 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_18069_length_5434_cov_3.258412, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000027 DAOOKS010000000
VERSION     DAOOKS010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5434
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18069_length_5434_cov_3.258412"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="PK112_00875"
     CDS             complement(<1..505)
                     /locus_tag="PK112_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007660492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HPC10636.1"
                     /translation="MNYFRLWLAMGVVIVASFAVLGYFGGDIYRQAPPVPDRVVTSDG
                     AVLFTGSDIKDGQNVWQSTGGQQVGTVWGHGSYVAPDWSADWLHRESEWILNEWARTE
                     YGVSAYTSLSGPDQAALRERLREEIRPNTFDPKTGDLVVSPVRARAIEAVGRHYAGLF
                     GGDPALGK"
     gene            764..1546
                     /locus_tag="PK112_00880"
     CDS             764..1546
                     /locus_tag="PK112_00880"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015188125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HPC10637.1"
                     /translation="MRTAFIFCAALLWATAAGAAVHTETVEYRHGKVVLEGYLAYDSS
                     LIGTGPVPGVLVVHEWTGLGDYVKIRARRLAELGYVAFAADVYGKGIRAKNADEAAKL
                     AQTYYQDRRLFRDRVQAALAVLRGQELSDDRRLAAIGYCFGGSAVLELARAGADVRGV
                     VSFHGILSTTQPAEPGTVKAKILVLGGADDPYVPDSQVAAFEEEMRRVGADWQLTEYG
                     GAVHSFTNPDAGNDASRGAAYNEAADRRSWEQMKMFFEEIFR"
     gene            1829..2212
                     /locus_tag="PK112_00885"
     CDS             1829..2212
                     /locus_tag="PK112_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 domain-containing protein"
                     /protein_id="HPC10638.1"
                     /translation="MAHEWQELTRLTGDKEVIVERVRLVVEGIALEGEFELPPLAKLT
                     AEDQLFVAAFVRSHGSIKKMEEVFGISYPTVKNRLNRIGAALSFAQVQVEPGDRREEL
                     LDRLERGEITVQDALSQLEKEQNHE"
     gene            2205..2627
                     /locus_tag="PK112_00890"
     CDS             2205..2627
                     /locus_tag="PK112_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006344828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10639.1"
                     /translation="MSEDRRRILNMLAEGKITADEAERLLEALGRTAERTAPETTGRT
                     AGARPKYLRVIVQPKQGQRDQVNIRIPLALLRAGVKLGGVVPDSVKGRINEALAEKGF
                     TVDLGKLNMEDLERLIDALGEMSIDVDDERESVRVCCE"
     gene            complement(2712..3311)
                     /locus_tag="PK112_00895"
     CDS             complement(2712..3311)
                     /locus_tag="PK112_00895"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HPC10640.1"
                     /translation="MKATFVAACVCLLILGAALSAQAQSEKPTLGLGYHGILAGDVLQ
                     GLSGRIWTSPKVGLEANLYYAHVGVDVKMEGEEGQEDDEYDISGTLTDFSGKFLYAVA
                     NHSQSKFYVGAELGYGHVSVEVETPGLPAALAEGFDVITLGPLMGAEFSFAGIPEMSV
                     DWELGYRTNLVDIEVADYDISLSMGVLAASVGVHYYFGR"
     gene            complement(4146..4796)
                     /locus_tag="PK112_00900"
     CDS             complement(4146..4796)
                     /locus_tag="PK112_00900"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HPC10641.1"
                     /translation="MFKRAAMFTLALLLIVSMPTWAQNQIKPVVYAGGGIDLLLSPDL
                     LKDYWKMGYGFGGGVGIQVKPNLELIGRVSFNTFPFDADKFLDDMDAPAGVSVDGLDF
                     RSLEFGADMKYIFMSDQANMMFKPFVVAGLGFANIKATDATITAEDEDDIEEVHLPFG
                     DASETDFSFNFGGGFDYMFAPKAGAWVDVRYTYIASEGDAISYLPIRAGVKFLFGN"
     gene            complement(5018..>5434)
                     /locus_tag="PK112_00905"
     CDS             complement(5018..>5434)
                     /locus_tag="PK112_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10642.1"
                     /translation="WFEVGFPYVVVATDQVNFWVRPGFQFASLDDRIFSPDSKVYDAD
                     DDFEEQGMDDVGLDAKFTQMTISLTPAAEWFISDNFSIEAGHGIKIQMLKFPDDKIPG
                     SDPAEYYYGDAGGETETSIVTFSADNVFFAFHFYFK"
BASE COUNT         1128 a         1602 c         1696 g         1008 t
ORIGIN      
        1 gcttgccgag cgccggatcg ccgccgaata gacccgcgta gtgccgtccg accgcctcga
       61 tggcgcgcgc ccgcaccggg gagaccacca ggtccccggt tttcggatcg aacgtgttgg
      121 ggcggatttc ctcgcgcaga cgctcccgga gagccgcctg atcggggccg ctcagggacg
      181 tgtaggccga cacgccgtac tcggtgcgcg cccactcatt gagaatccac tccgattccc
      241 ggtgcagcca gtcagccgac cagtcggggg ctacgtacga gccgtgtccc cagaccgtgc
      301 ccacctgctg gccgccggtg gactgccaga cgttctgccc atccttgatg tcgctgccgg
      361 tgaacagcac cgcgccgtcg gacgtgacga cccggtcggg caccggcggg gcctgacgat
      421 agatatcgcc gccgaaataa ccgaggactg cgaaggaggc gacaatcacc acacccatcg
      481 cgagccacag tcggaagtag ttcacaccaa agctcctttc atatatgaga cctcgccatg
      541 tcggctcagc cgccccggtg cgggttgatt ccccgcacgt attacggagc aggcggcgga
      601 cagtttgtcg cggacgccgg gcacgggggc cggtacgcac cgccgcgcga agtatcgact
      661 gaataggcct atgatagcct tgacattagt caacggccag tgttttttgt gcgcggaccc
      721 ggcgaacagt tcgccggcgc atccctgaga aaggacccga ataatgagga ctgcattcat
      781 cttctgtgcg gcgctgcttt gggcaacggc ggccggcgcc gcggtgcaca ccgaaaccgt
      841 cgagtaccgg catggcaagg tcgtcctcga gggatacctg gcctatgatt ccagcctgat
      901 cggcacagga ccggtccccg gcgtgctggt ggtccacgag tggaccgggt tgggcgacta
      961 cgtcaagata cgcgcccggc ggctggccga actcggctat gtggctttcg ccgccgacgt
     1021 ctacggcaaa gggatccgtg cgaaaaacgc cgacgaggcg gcgaaactgg cccagaccta
     1081 ctaccaggac cgccgccttt tccgtgaccg cgttcaggcg gcgctcgccg tgctgcgcgg
     1141 gcaggaactc tccgatgatc gccggctggc cgcgatcgga tactgcttcg gcggctccgc
     1201 ggtgctggag ctggcgcggg cgggggccga cgtccgcggc gtggtgagtt tccacggcat
     1261 tctctcgaca actcagccgg ccgagccggg gacggtcaag gccaaaatcc tcgtgctcgg
     1321 cggagccgat gatccgtacg taccggattc gcaggtggcc gcgttcgaag aggaaatgcg
     1381 ccgggtcggc gccgactggc aactgactga gtacggcggg gcggtgcaca gcttcaccaa
     1441 cccggacgcc ggcaacgacg cgtcgagggg cgccgcgtat aacgaggcgg ccgaccggcg
     1501 gtcgtgggag caaatgaaga tgttctttga agagatcttc cggtagcccg caggacaggc
     1561 atactcggcg gcgctctgcc tgcttcagcg gcgcgagaac cgatatgggt cggttttcgc
     1621 gccgcttctt cattaatgga tgcgctgccc agagcgttct cgcttaagta aatggggaat
     1681 aatggcttgc cgattgagaa caaggcctgt tgatgagcgg agaggtctga atccacaaat
     1741 tcttacaatg aaaatcaatt ctcgtattaa cttctacgac tattgatcgt atacaatatt
     1801 aaataactga aacaatggga agacacatat ggcccatgaa tggcaggaat tgacccgcct
     1861 caccggcgat aaagaagtga tcgtggagcg cgtgcggctg gtcgtcgagg gcattgcgct
     1921 ggaaggggag ttcgaattgc cgccgttggc gaaacttacg gccgaggatc agctgtttgt
     1981 cgccgctttc gtgcgcagcc acggttcgat caagaagatg gaggaggtgt tcgggatcag
     2041 ctatccgacg gtcaagaacc ggttgaaccg gatcggggcc gcgctgtcat tcgcccaggt
     2101 gcaggtcgag cccggcgatc gccgggaaga actgctcgac cggctcgagc ggggagaaat
     2161 cacggttcag gacgcgttgt cgcaattgga gaaggagcag aatcatgagt gaggatcgtc
     2221 gccgcattct caacatgctg gccgaaggga aaatcacggc cgatgaggcc gagcggctgc
     2281 tcgaggcgct cggccggacg gcggagcgca ccgctccgga gacgacgggg aggacggccg
     2341 gcgctcggcc caaatacctc cgggtaatcg tgcagccgaa acaggggcag cgggatcagg
     2401 tcaatatccg gatccccctc gctctgctcc gggccggggt gaaactcggc ggcgtagtgc
     2461 cggacagcgt gaaggggcgg atcaacgagg cactggcgga gaaagggttc acggtcgacc
     2521 tgggcaagct gaacatggaa gatctcgagc ggctgatcga cgcgctcggg gagatgagta
     2581 tcgacgtgga tgacgagcgc gagagcgtcc gagtctgctg cgagtgaagc gccgccgctc
     2641 ccgccctctc cggtgcgagg agcccggcgg cagggccggg ctccgttgcg cgaacctgga
     2701 acagagtgcg gctagcggcc gaagtagtag tgcacgccga ccgaggccgc gaggacgccc
     2761 atcgacagcg agatgtcgta gtcggcgacc tcgatatcga ccaaattcgt gcgatagccg
     2821 agctcccagt caaccgacat ctcggggatt ccggcgaacg agaactcggc gcccatgagc
     2881 gggccgagcg tgatcacgtc gaatccctcg gccagcgcgg ccggaagccc cggggtctcg
     2941 acctcgaccg acacatggcc gtaaccgagt tccgcgccga catagaactt gctctggctg
     3001 tgattggcta ccgcgtagag gaacttgccc gaaaagtccg tcagcgtgcc gctgatatcg
     3061 tactcgtcgt cttcctgccc ttcctcaccc tccattttca cgtccacgcc cacgtgggcg
     3121 tagtagaggt tggcctcaag gccgactttg ggcgatgtcc agattcggcc gctcagcccc
     3181 tgcaggacgt cgccggccag aattccgtgg taacccagac caagcgtcgg cttttcactc
     3241 tgcgcctgcg cggacagcgc cgcgcccagg atcagcaggc agacacaggc ggccacaaac
     3301 gttgctttca taagtcactg cctttcgact aagtggtgat tcccctgaac cggcccgacg
     3361 agatcttcga gtcggcacga cgtcagacca tggagcgaat tggttcgttc ccctcctttc
     3421 ctccgctagt gcggcgccct cgacaagagc ggcacccttc ggtgatgaac gctggtgttt
     3481 tcgtctatgg gtacctggtg ttgagagacg atgccgaata cccctctaac cgggatgccg
     3541 gaccgactgc cgcaggctgc cttcggactc tgccgcggta gaggtacacg aatgaggaaa
     3601 ttctaccgtg ccgcggcccg gctgtcaaca tattttaccc ggtcgtccat tttagtcgac
     3661 gattggcggg gggagcgggc agggctggga gttcgcagtt gatgaagata acctgcggct
     3721 ggacgccggc gagctgactg cggcaccggt ccccgcgggg cggaatctga gccgcggcgg
     3781 ccacttctcc catctgtcgt tcggccgaga cgctgacaga aggataaggt caatgccgcg
     3841 atttcgccga gcctgattgg aacgacagca ggcttagcgg ctttctgtcg ggcagtttga
     3901 atgggcgatc cacgaggcgc ccgtagggga gaggtcgctg ccgtcccggt gaagacactc
     3961 gccggcgatc ttgaacctct cacccatgcg cttcgcaatc gctgttgttt tcagacaaac
     4021 cgtggtaagg tgcaggtgcg gagtcgttgc gagagagccc aagcccacct gccgctgcgg
     4081 aggtatcaaa agaaccgggc tcgaattgcc aagcccggtt ctcagtggac ctatcgtaca
     4141 gggaatcagt tgccaaagag gaacttcacg cccgcacgaa tcggcaagta ggagatggca
     4201 tctccttcgc tggcgatata ggtgtagcga acatccaccc aggcgccggc ctttggcgcg
     4261 aacatgtagt caaagccccc gccgaagtta aacgagaaat cggtttcgct ggcatcgcca
     4321 aaaggcaaat gaacttcctc tatgtcatcc tcgtcctcag ccgtaatggt cgcatccgtg
     4381 gccttgatgt tcgcaaagcc gagcccggcg actacgaatg gcttgaacat catattcgct
     4441 tgatcagaca tgaagatata cttcatatcg gctccgaact caagggagcg gaagtcaagc
     4501 ccatcgacag agactcccgc cggggcatcc atgtcgtcca ggaatttgtc ggcgtcaaag
     4561 ggaaaagtgt tgaaggacac gcgaccgatg agttcaaggt taggcttgac ttgaatgccc
     4621 acgccgccgc cgaatccata tcccatcttc cagtagtctt tgagcaaatc aggcgacagg
     4681 agcaagtcaa ttccaccgcc cgcatacacc accggtttta tctggttttg ggcccacgtg
     4741 ggcatggata cgatcagcaa caaggccaga gtgaacattg cagcacgctt aaacatggag
     4801 cacctcctga gtgttgggta atgaacctga ctatcctttt ccggttctcg cctatggcca
     4861 gacttccgtg tcaaactact ttcagtattc agtcaaagct acaaaaaaac cgggcgccgc
     4921 gatcggggtt ctgaggacaa acaaatataa caaaggggca ggatcggatg accctgcccg
     4981 tgtcgtttgc actaatcacc tcagcgaagg ggacggccta cttgaagtag aagtgaaatg
     5041 cgaagaagac attgtcggct gagaaggtca cgatgctggt ctcggtctcg ccgccagcat
     5101 caccgtagta gtactcggcc gggtcgcttc cggggatttt gtcgtccggg aacttgagca
     5161 tctggatctt gatcccgtgg ccggcctcaa tcgagaaatt gtcgctaatg aaccactcag
     5221 ctgcgggagt aaggctgata gtcatctggg taaacttggc gtcgaggccg acatcgtcca
     5281 tcccctgttc ctcgaagtcg tcatctgcat catagacttt cgagtcgggg ctgaagatgc
     5341 ggtcgtccaa cgaagcgaat tggaatcccg ggcgaaccca gaagttcacc tgatccgtcg
     5401 caacgaccac atacgggaat ccaacctcga acca
//
LOCUS       DAOOKS010000028         5426 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_18108_length_5426_cov_3.666356, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000028 DAOOKS010000000
VERSION     DAOOKS010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5426
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18108_length_5426_cov_3.666356"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /gene="flgG"
                     /locus_tag="PK112_00910"
     CDS             <1..590
                     /gene="flgG"
                     /locus_tag="PK112_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013681739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HPC10643.1"
                     /translation="IGYGTRASATVRQYAEGNLTPTGNPLDLAIDGDGFFQVQMPDGT
                     TAYTRDGAFKISGDGQIVTSDGYFLLPQVSIPQDATALAVGTDGTIEVMVAGQSDPVE
                     IGQIELARFVNPAGLLAVGRNLLTPTRASGDALTDVPTQSGLGQIDQGYLEMSNVKVV
                     DEMVNMIVAQRAYEMNSKAIQTADDMAQIANNLKR"
     gene            596..1321
                     /gene="flgA"
                     /locus_tag="PK112_00915"
     CDS             596..1321
                     /gene="flgA"
                     /locus_tag="PK112_00915"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HPC10644.1"
                     /translation="MTELIRKIILILLFLAFAAAGGAVPVTQAAIDELVRMYGLDTTM
                     YAIDVDLNRLHTTEVGTIPATAPGAAAEPSRLTLRPLSAKEPLGLFTAEATVEQGGAP
                     VESAMIRFRITKFAQVLVAADRIDMRAPIAPEQVEVRRVDVTTMYEKPLTAVGELAGM
                     RAARMIRKGTVLTTACVEIPPDVESGRTVEIVYRDGQCRIATRGTALQAGRVGEAIKV
                     RNDSSGKVITARVADAGAVTVEP"
     gene            1334..1921
                     /locus_tag="PK112_00920"
     CDS             1334..1921
                     /locus_tag="PK112_00920"
                     /inference="COORDINATES: protein motif:HMM:NF014195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HPC10645.1"
                     /translation="MKLLKALIILLILLLLPYSLKLKGQDFGQSRSLFTDVKANRVGD
                     LLTVLIYENNTAQQNAQTKTEKSTEASTSGGPGVGSLDFIKLFGADAKNESTFDGKGS
                     NSRNGSLRARMSVTVVEVRANGDLVIEGSRTIGINGERETLHLAGVVRQKDVTPQNTV
                     DSYLIGDAEIHYTGKGSLNSGTRPGIFTRIINWLF"
     gene            1932..3068
                     /locus_tag="PK112_00925"
     CDS             1932..3068
                     /locus_tag="PK112_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HPC10646.1"
                     /translation="MVGTMCHRLATPTGLVIALAILLAWATTAEANKVRIKDIADFRN
                     EQQVDLIGYGLVIGLDGTGDGTGTQFTVQSLVNMMQRMGMTVDVEKVKVKNVAAVMVT
                     GRITSSQTEGGLIDVTVSSIGDAKSLQGGTLLMTPLSGLDGTVRAVAQGPVSIGGFNI
                     QVDESNKLYNNYTLVGRVPNGGKVTAPPTPENATEREIYLSLRSPDYTTAHRVAERIN
                     IKYGLLAYPVDAGSVRLVVPDSLSHPTARIEFLSDVGQLQVVPDNPARVVINERTGTI
                     VAGEHVTIDAVAIAHGSITVNIESTPVISQPEPFSQGETVVTSDARIQVQDEKARVVE
                     LKPAVYLSDVANALNRIGATPRDIIAIFQALKQSGALRAELVIL"
     gene            3704..4108
                     /locus_tag="PK112_00930"
                     /pseudo
     CDS             3704..4108
                     /locus_tag="PK112_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012749457.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
     gene            4139..4627
                     /gene="flgN"
                     /locus_tag="PK112_00935"
     CDS             4139..4627
                     /gene="flgN"
                     /locus_tag="PK112_00935"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FlgN"
                     /protein_id="HPC10647.1"
                     /translation="MVDRLIDIIGREAALFERFLLLLERQQRALVANDQEELTRVTDL
                     QRETLAESRRLHEEREGVLADIRRTNAIEGDLTVTRLVAMVDADRADRLAALRGTILD
                     LNERILRLRNQNVLLLNRSREYIRRTMELLSRIASPRAETYAPAGAAGAEARTVALDR
                     RV"
     gene            4627..>5426
                     /gene="flgK"
                     /locus_tag="PK112_00940"
     CDS             4627..>5426
                     /gene="flgK"
                     /locus_tag="PK112_00940"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HPC10648.1"
                     /translation="MSGLMAGLEIGKRALLSHQVNLQTIGHNIANVNTPGYSRQRVTI
                     SSTYPSETAAGLVGTGVTVEDIRHVRDLFLGDQYRQENKTLGQWTYKQKILSQVESLF
                     AEPNDNTLADRLNEFWDSWDDLSKNPNATNREGVIGAARLLTESFHTLARDINKLRDS
                     VDADLATYTSQINQTTGEIAALNRQIVTQEVDGSRASDLRDRRNQLIDELSSLLDVNT
                     IEDANGAVRVMVGAMEIVTGLDTIPIATRILNDNGRMTHELVWRNTAV"
BASE COUNT         1046 a         1746 c         1881 g          753 t
ORIGIN      
        1 cgatcgggta cggcacgcgc gcctcggcga ccgtccggca gtacgcggag gggaacctga
       61 ccccgacagg aaatccgctc gacctggcga tcgacggcga cgggttcttc caggtgcaga
      121 tgccggacgg gaccacggcc tacacgcgcg acggggcctt caagatttcg ggcgacggcc
      181 agatagtcac ttccgacgga tacttcctgc tgccgcaggt gtcgatcccg caggatgcga
      241 cggcgctggc ggtgggcacc gacgggacga tcgaagtcat ggtggcgggg cagagcgatc
      301 cggtggagat cggccagatc gaactggcgc ggttcgtcaa cccggccgga ctgctcgcgg
      361 tcgggcgcaa cctgctcacg cccacgcggg cctcggggga cgccctgacc gacgtgccga
      421 cgcagagcgg gctgggccag atcgaccagg ggtatctcga gatgtcgaac gtgaaagtgg
      481 tcgatgaaat ggtcaacatg atcgtcgccc agcgggccta cgagatgaac tcgaaggcga
      541 ttcagaccgc cgacgacatg gcgcagatcg cgaacaacct caagaggtag cggccatgac
      601 cgagctgata cggaaaatca tcctgatcct gctctttctc gcgttcgcgg ctgccggcgg
      661 ggccgttccg gtcacgcagg cggcgatcga tgaactggtg cgcatgtacg ggctcgacac
      721 gacgatgtat gcgatcgacg tggacctcaa ccggctccac acgacggagg tcgggaccat
      781 ccccgcgacc gcgccgggcg cggccgccga gccgtcccga ctgaccctgc ggccgctctc
      841 ggccaaagag ccgctcgggc tgtttacggc ggaggcgacc gtggagcagg gcggggcgcc
      901 ggtcgagagc gcgatgatcc gctttcgcat cacgaaattc gcccaggtcc tggtggcggc
      961 cgaccgcatc gacatgcgcg cgccgatcgc cccggagcag gtcgaggtgc ggcgggtcga
     1021 cgtgacgacg atgtacgaaa agccgctcac cgcggtcggc gaactggccg ggatgcgggc
     1081 ggcccgcatg atccggaagg ggacggtgct gacgacggcc tgcgtggaga tcccgccgga
     1141 cgtcgaatcg gggcgcacgg tggagatcgt gtaccgcgac ggtcagtgcc ggatcgccac
     1201 ccgcgggacg gcgctgcagg cggggcgggt cggggaggcc atcaaggtga gaaacgattc
     1261 atcgggcaaa gtcatcacgg cccgcgtcgc cgacgccggc gcggtgaccg tggagccata
     1321 ggaaggcgga gcgatgaaac tgctcaaagc gctcatcatc ctgttgatcc tgctgctgct
     1381 tccgtactcg ctgaagctca aggggcagga tttcggacag agccgttcgc tgttcacgga
     1441 cgtaaaggcc aaccgggtcg gggacctgct caccgtgctc atctacgaga acaacacggc
     1501 acagcagaac gcccagacca aaaccgagaa gagcacggaa gcctcgacct cggggggacc
     1561 gggggtcggg agcctcgact tcatcaagct gttcggggcg gacgcgaaaa acgagagcac
     1621 cttcgacggc aagggatcga actcgcgcaa cgggagtctc cgagcgcgca tgtcggtgac
     1681 ggtggtcgag gtgcgcgcca acggcgacct cgtgatcgaa ggatcgcgga cgatcgggat
     1741 caacggcgag cgtgagacgc tccacctcgc cggggtggtg cgccagaagg acgtcacccc
     1801 gcagaacacg gtggattcgt acctcatcgg cgacgccgag atccactaca cggggaaggg
     1861 gagcctgaac agcgggactc ggccgggcat tttcacccga atcatcaact ggctcttcta
     1921 ggaggcggga cgtggtggga acaatgtgcc atcgactggc gacgccgacc gggctcgtga
     1981 tcgcgctggc gattctcctc gcctgggcga caactgcgga ggcgaacaag gtccgcatca
     2041 aggatatcgc cgacttccgc aacgaacagc aggtcgacct gatcggctac ggactggtga
     2101 tcggtctcga cggaacgggg gacgggaccg ggacccagtt caccgtgcag tcgctggtca
     2161 atatgatgca gcggatggga atgacggtgg acgtcgagaa ggtcaaggtc aagaatgtgg
     2221 cggcggtcat ggtcaccggg cggatcacga gcagccagac ggagggcggg ctgatcgacg
     2281 tgaccgtgtc gtcgatcggg gacgcgaaat cgctgcaggg ggggacgctg ctgatgaccc
     2341 cgctgagcgg acttgacggg acggtgcggg cggtggccca ggggccggtg tcgatcggcg
     2401 ggttcaacat tcaggtcgac gaatcgaaca agctctacaa caactacacg ctcgtggggc
     2461 gggtgccaaa cggcggcaag gtcaccgctc cgccgacccc tgagaacgcc accgagcgtg
     2521 aaatctacct ctctctgcga agccccgact acaccaccgc tcaccgggtg gcggaacgga
     2581 tcaacatcaa gtacggcctc ctcgcctacc cggtcgatgc cgggtcggtg cgactggtgg
     2641 tgcccgactc gctgtcgcac ccgacggcgc ggatcgagtt cttgtccgac gtcggccagc
     2701 tccaggtggt gccggacaac ccggcgcggg tggtcatcaa cgagcggacg gggaccattg
     2761 tggccgggga gcacgtgacg atcgacgccg tggcgatcgc ccacgggtcg atcacggtca
     2821 atattgaaag cacgcccgtg atctcacagc ccgaaccctt ctcgcagggg gagacggtgg
     2881 tgacgagcga cgcgcggatc caggtccagg atgagaaagc gcgggtggtg gaactcaagc
     2941 cggcggtgta tctgtcggac gtggccaacg ccctcaaccg gatcggggcg accccgcgcg
     3001 acatcatcgc catcttccag gcgctaaagc agtccggggc gctccgcgcg gagctggtga
     3061 ttctgtaatg tcaaccctgc gacccataga cgcgccggtc gtcgcgaacg gcgacctcga
     3121 tcggctgcgc ggccccgccg ctacccccga gcaggagaga gcgcgtctgc gcaaggcggc
     3181 ccaggaattc gaggccctct tcatgtacca gatgctcaaa gcgatgcggc agacggtggg
     3241 agagagctcc ctggcgaaaa acctccccct cggcggaagc ctgggcaagg acacctacac
     3301 tgaccttttt gatttggagc tcgcgcgcgg catggccacc ggcgggcacg ggtcgatctc
     3361 ggatatcctc tgccgagcgc tggaaaaaac agtgaccgcg gattccgggc agacgcccgt
     3421 gccgacggcg atccggccgc tggagagcgg cgaccgggag cgcaaggcga ttgccataca
     3481 ggggccgcgg ctgccgctgg accgggggga gccgcacccg gcgcccctgg cgccgaaacg
     3541 gccgccgccg cttccgcccc ggccgaatca cgcggcgccg cccgctgccc cgccgccgcc
     3601 gcgcgcgccg gtgcaggcgc cgcccgccga gccgatgccc gaaagcgccc cggccgacgc
     3661 ggccgatccc atccaggcgc ggttcggacg gtggatcgac gaggcggccg aggccaaccg
     3721 gctcgattcc gcgctcatcc gcgcggtcat cgccgccgag tcgggcggga atccgcgcgc
     3781 cgtatcgccg gccggcgcga aaggactgat gcagctcatg gataccaccg ccgcggacat
     3841 gggtgtgaaa gagccgttcg atgcgcgcga gaacatcatg tcgggaacca aatacctgcg
     3901 ccggctgatc gaccgcttcg gcgatctcaa gctggcgctg gcggcctaca acgcgggacc
     3961 gggcacggtc gagcggcacg gcggcgtgcc gccctacgcc gagacccggg cctacgtgga
     4021 gaaagtgctg gattcggccc gggcgctcgc ggccgacggg ggggctccgg acggggagtc
     4081 taaagccccc ggcggcgcgc gccgataaca ccacaggggt cttgcaggat gcagaaacat
     4141 ggtggatagg ctcatcgaca tcatcggcag ggaagcggcc ctcttcgaac gctttctcct
     4201 tttgctcgag cggcaacagc gcgccctggt cgccaacgac caggaagaac tcacgcgggt
     4261 caccgatctc cagcgggaga cgctcgcgga aagccggcgg ctgcacgagg agcgggaagg
     4321 cgtgctcgcc gacatccggc ggacgaacgc gatcgagggc gacctgacgg tgacgcggct
     4381 ggtggcgatg gtcgatgccg accgcgccga ccgcctcgcc gcgctccggg gcacgatcct
     4441 cgacctcaac gagcgaatac tccggctccg gaaccagaac gtcctgctgc tcaaccggtc
     4501 gcgcgagtat atccggcgga ccatggaact gctttcgcgg attgcgagcc cccgggcgga
     4561 aacgtacgcg ccggcgggcg ccgcgggggc cgaggcccgc acggtcgccc tggatcggag
     4621 ggtgtgatgt ccggactaat ggcgggactg gaaatcggaa aacgggcgct gctgtcgcac
     4681 caggtcaacc tgcagacaat cggccacaac atcgccaacg tcaatacacc cggctactcg
     4741 cggcagcggg tcacgatcag ctcgacctac ccgtcggaaa cggccgccgg actggtcggg
     4801 accggcgtca cggtcgagga tatccgccac gtgcgcgacc tgttcctcgg cgaccagtac
     4861 cgccaggaaa acaagaccct cggccagtgg acctacaagc agaaaattct gagccaggtg
     4921 gagtcgctgt tcgccgaacc caacgacaac accctggccg accgtctcaa cgagttctgg
     4981 gattcctggg acgacctgtc aaaaaacccc aacgcgacca accgggaagg ggtgatcgga
     5041 gcggcccgcc tgctgaccga gagtttccac accctcgccc gcgacatcaa caagctgcgc
     5101 gattcggtcg acgccgacct ggccacctac accagccaga tcaaccagac gaccggcgaa
     5161 atcgccgccc tcaaccgcca gattgtcacg caggaagtgg acgggtcgcg ggccagcgat
     5221 ctccgggacc gccgcaacca gttgatcgac gagctctctt cgctgctcga cgtgaacacg
     5281 atcgaggatg cgaacggagc ggtgcgggtg atggtcgggg cgatggagat cgtgaccggt
     5341 ctggacacga tcccgatcgc gacgcggatt ctcaacgaca acggccgcat gacccacgaa
     5401 ctggtgtggc ggaatacggc ggtcga
//
LOCUS       DAOOKS010000029         5409 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_18201_length_5409_cov_2.636907, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000029 DAOOKS010000000
VERSION     DAOOKS010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5409
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18201_length_5409_cov_2.636907"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            369..959
                     /locus_tag="PK112_00945"
     CDS             369..959
                     /locus_tag="PK112_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007065578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diheme cytochrome c-553"
                     /protein_id="HPC10649.1"
                     /translation="MNQRAILVGRGRLVAAGVVMVLAMPLAWLWAQGAPTYTAEQVKR
                     GEYLVVIGGCHDCHTPKKMTERGPEPDFARSLSGHPAQEKLPAVPAGAITPTGWMALT
                     NGNLTAWAGPWGVSFALNLTPDVKTGIGGWTEEMFIQALRTGKHLGMGRPILPPMPWQ
                     NLSHATTEDLRAIFAYLKSLPVIENRVPDPIPPAVH"
     gene            complement(1098..1352)
                     /locus_tag="PK112_00950"
     CDS             complement(1098..1352)
                     /locus_tag="PK112_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10650.1"
                     /translation="MTHGSRNLLSSLGRRRLLGLLLVAGGAVLSGCGLRSAGEKARAR
                     GRILARGGGASIPAGLEVTLQFPLLEAIYGRRSRRFFRGA"
     gene            1657..2478
                     /locus_tag="PK112_00955"
     CDS             1657..2478
                     /locus_tag="PK112_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10651.1"
                     /translation="MTGLSAVALAVAGMAAIAGTEAAPPGAALLPPSLNKLYPPEAPA
                     PVYLIEMHALAAPLSGMMSDLMEGDHANAMANFEAFRAQYAKVAELVPEWRHEFPTGP
                     VETLATALRSGDQGAVMRAGDAVGRACHGCHLQTMVPAQHRFHWGDFGAISVTDPVIG
                     QDMPFAQFMHMLDGDLAGIGNDLKQGQPENARRHAEGLAARYATLEEVCGVCHDTDRK
                     YYVDEKVMQMIADLPAALENGKPENLGALLQGIGQESCQKCHLVHIPAAYARAAH"
     gene            complement(2565..3695)
                     /locus_tag="PK112_00960"
     CDS             complement(2565..3695)
                     /locus_tag="PK112_00960"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPC10652.1"
                     /translation="MKRWRILVVDDEPGVLRAVQRVLGSAHDLTLCDSSPAARDLARR
                     SRPHLALIDIRMPHLDGFDLMTQLKEDDPDLEVILMTGSMDDTDEKLVRAVRNRAFYY
                     ITKPFDRDALLALVDRCLELKRLELSNRAYADHLRRQLLAAGAFQRTMLPPASAHLNG
                     VELRAGYYPCDQVAGDYYDYAVTGGDLVTLLLADVVGHGASAAMFTALVKSAFHRAAD
                     RGYAPLRVARILLDDLSAFESDQYVTLLCARLSRSGRTIEYVNAGHSGGLIVGGDETL
                     LPLEVTSPLISPVVPPSALRTATIPLPADSLLLLFTDGAAEAWSDDEMFGLERLYALA
                     RTGWACRDALPQHLQDGVRRFLGDRSQTDDITFLAAWPASAR"
     gene            complement(3692..>5409)
                     /locus_tag="PK112_00965"
     CDS             complement(3692..>5409)
                     /locus_tag="PK112_00965"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPC10653.1"
                     /translation="RTAHAGVVMFDGTIDKMHADLYKVLFDLRGTLRDYGEPRKMLIY
                     GMRRAVEFLAADAGAVATLSASAHTADLLFTTRTSTEWNVGLLNDFIKRREPRIPDDV
                     VLARVERRAATWGAIAVRLPHPKEAFHLRALGLIAEAVREGIAQYDDRQIRRLRHRLE
                     QKVVNRREPKDIIYLILHGIRSLTRYDHSATFLATNGPDGPLQIIAEQIAWTKAKSRR
                     IGLTVPLAAELRDELSRGGIHLHRQGGHGWQPVFGAVRTPGLIPALCCTCPRGASAPE
                     ENAVLCAVIPAPDGSLGILKIAFQRADIPGAYEIELVENFTPLLSLALQFHHRTEVLT
                     EQVVQAERKSALADLSRGIAHDVNNAIGSVLPLAQQIRADIARGELDQRELLADLDVI
                     ESGMLACRRIFAGLLSVARSGDRTIGEANLRRAVDGALNVVRSRLDRHSIAVELNIPP
                     ELSAIGANQGHITQVFLNLFANSIEAMPHGGRLAVSAVEKGPEIEARVEDSGGGIPPD
                     LLDRVADAFITTKESGHGLGLAVCRGILWNIGGELRIDSTVGQGTCVTLRLPTQSSRR
                     QERLP"
BASE COUNT          901 a         1746 c         1948 g          814 t
ORIGIN      
        1 cctgggcgaa aaagtgcggt tggcgtcggg actccagata ctcgaaggag tggctgtgga
       61 catcgagccg ggcggggggc tggtgatcga gcggggggga aagcgccacg tggtcacggc
      121 cggggatgtg acggtggtaa aggagtaggg gggaggcgac gcccgtcggg accgcggccg
      181 gcggggagat gatggtgggg aggggggtcg gggatgggtt ccccgggccg gcggagggga
      241 gatccccaat cgacttgacg gaccgattta gtctattatt tcgcttgacg gcggcgcccg
      301 gcgtgttata tttccgcccg ttccaccact gcggacttac cggaaacact gctaacagag
      361 gagcaacctt gaatcagcga gcaattctcg tgggccgcgg ccggttggtg gccgcgggcg
      421 tggtcatggt gctggcgatg cccctcgcct ggctgtgggc ccagggcgcg ccgacctaca
      481 cggccgagca ggtcaagcgg ggggagtatc tcgtggtgat cggcgggtgt catgactgcc
      541 acacgccgaa gaagatgacg gagcgcggtc ccgagcccga tttcgcccgg tcgctgagcg
      601 gccacccggc ccaggagaaa ctgccggcgg tgccggcggg cgcgatcacg ccgaccggct
      661 ggatggcgct gacgaacggg aacctcaccg cctgggcggg gccgtggggg gtcagtttcg
      721 ccctcaacct cacgcccgac gtgaagacgg ggatcggcgg gtggaccgag gagatgttca
      781 tccaggcgct gcggaccggc aagcacctgg gcatgggccg gccgattctc ccgcccatgc
      841 cctggcagaa cctctcccac gccaccaccg aggacctgcg ggcgatcttc gcctacctga
      901 aatcgctccc ggtgatcgag aaccgggtcc ccgatccgat tccgccggcc gtgcattgag
      961 atatcccgga gcggaccacc gcgccggaaa gcccaaatcg acggcgggag ccgatgctcc
     1021 cgccgtttcc tttgctcgag ctcctcaagc gacaacggcg ggtgctccga cttgcaggcc
     1081 agcacgccgt cggggatcta cgccccccgg aaaaagcgcc gggaccgccg cccgtagatc
     1141 gcctcgagaa gggggaactg gagagtgacc tcgaggcccg ccgggatgga ggcgccgccc
     1201 ccgcgcgcca ggattcgtcc ccgggcgcgc gctttttccc ccgccgatct caggccgcag
     1261 ccggagagca ccgcgccgcc ggccaccagc aacaggccga ggagccgccg ccggcccaga
     1321 gaactcaaca agttgcggga accgtgcgtc atgcgtcctt cctccgtgtg gggatgtgcg
     1381 ggaccgggcc ggcaaaggcg cgggaaggcc gcccgcccgc cgcccggtcg gaccaaccgg
     1441 tttttttgcg gctcttgacg aaagtcaatt tccgccgaac cctgtcctgc gtactgtccg
     1501 gggcggttct ccgcgcccgg agcggcggcg ggaaccgccg tgccgacgtt tgcagcggga
     1561 ataactcatt ttctaccaga ccaactcgcg gtcggccgca atggggcggc cgccgcaaac
     1621 gtgatgtgag aggagtaacg tatgaggagg ttggttatga cgggactttc agcggtggcg
     1681 ctggcggtgg cgggaatggc cgccattgcc gggaccgagg cggcgccgcc gggggccgcg
     1741 cttctgccgc cgtcgctgaa caagctctat cccccggagg cccccgcgcc ggtgtacctg
     1801 atcgagatgc acgcgctggc ggcgccgctg tcggggatga tgtccgacct gatggaaggg
     1861 gatcacgcga acgcgatggc caatttcgag gccttccgcg cccagtatgc aaaggtggcc
     1921 gagctcgtcc cggagtggcg gcacgagttc ccgacggggc cggtggagac gctggcgaca
     1981 gcgctccgca gcggcgacca gggagcggtg atgcgggccg gtgacgcggt cggccgcgcc
     2041 tgccacggct gccacctcca gactatggtg cccgcccagc accgcttcca ctggggagac
     2101 ttcggcgcga tctcggtcac cgatccggtg atcggtcagg atatgccgtt cgcccagttc
     2161 atgcatatgc tcgacgggga tctcgcgggg atcggcaatg atctgaagca ggggcagccg
     2221 gagaacgccc gtcggcatgc cgagggactg gccgcccggt atgccacgct cgaagaagtg
     2281 tgcggcgtgt gccacgacac cgatcggaag tactacgtcg acgagaaggt gatgcagatg
     2341 attgcggacc tccccgcggc cctcgagaac ggcaaacctg agaacctggg cgcgctgctg
     2401 cagggaatcg ggcaggaaag ctgccagaag tgccacctcg tacacatccc ggcggcgtac
     2461 gcccgggcgg cgcactgagg cgacacggtt cggccgggtg gcgcggccgc tccccgcggg
     2521 gaagccatcc ggccgagacc gaaattcgac tcaagcggcg aaagtcagcg ggccgacgcc
     2581 ggccaagccg caagaaaagt gatgtcgtcc gtctggctcc ggtccccgag gaaccggcgg
     2641 acaccgtcct gaaggtgctg cggcagcgcg tcgcgacacg cccaccccgt tcgggcgagc
     2701 gcgtacagcc gctccaggcc gaacatctca tcgtcgctcc aggcctccgc ggcaccatcg
     2761 gtgaacaaca gcagcagcga atcggcgggc aacgggatcg tcgccgtccg cagagccgac
     2821 gggggtacca ccggtgaaat cagcggagac gtcacctcga gcgggagtag ggtctcgtcg
     2881 ccgccgacga tgaggccgcc ggaatgaccg gcgttgacat actcgatcgt ccgtccggac
     2941 cgggagagcc gggcgcacag cagggtcacg tattgatccg attcgaacgc ggagaggtcg
     3001 tccaggagga tccgcgccac ccgcagaggc gcatagccgc ggtccgcggc gcggtggaaa
     3061 gcggatttca ccagcgccgt gaacatggcc gccgacgcgc cgtgcccgac cacatcggcc
     3121 aacaggagag tgacaagatc ccccccggtg acggcgtagt catagtagtc gccggcgacc
     3181 tggtcgcagg ggtagtagcc ggcgcgcagc tcaacgccgt tgaggtgggc cgaagcgggc
     3241 gggagcatgg tcctctggaa ggcgccggcc gcgagcaact gccgccgcag gtgatcggca
     3301 tacgcccggt tgctcagttc gagccgcttg agttcaaggc aacggtccac cagcgccagg
     3361 agcgcatccc gatcgaacgg cttggtgatg tagtagaagg cccggttgcg caccgcccga
     3421 acgagcttct cgtccgtgtc gtccatgctg ccggtcatca ggatgacctc gagatcgggg
     3481 tcgtcctcct tgagctgagt catcaggtcg aaaccgtcga ggtggggcat gcgaatgtcg
     3541 atcagggcca ggtgcggacg gctgcggcgg gcgagatccc gggcggccgg cgaggaatcg
     3601 cacagagtca ggtcgtgggc gctcccgaga acccgctgca ccgcccgcag cacccccggc
     3661 tcgtcatcga ccaccaggat ccgccagcgt ttcatgggag gcgctcctgg cggcgggacg
     3721 actgggtggg gagccgcagg gttacacagg tcccctgacc gacggtgctg tcgatgcgga
     3781 gttcgccccc gatgttccac agaatccccc tgcagaccgc caggccgagg ccgtgcccgg
     3841 actccttggt cgtgatgaag gcgtccgcga cccggtcgag gaggtcgggc gggatgccgc
     3901 cgccgctatc ctccacgcgc gcctcgatct cgggaccctt ttcgacagcg ctgacggcga
     3961 gccgcccccc gtgcggcatg gcctcaatgc tgttggcgaa caagttgagg aacacctggg
     4021 tgatgtgccc ctggttggcg ccgatggccg agagctccgg ggggatgttc agttcgacgg
     4081 cgatgctgtg gcgatcgagg cggctgcgca cgacgttgag ggcgccgtcg accgcgcggc
     4141 ggagattcgc ctccccgatc gtgcggtcgc cggagcgggc caccgagagc agccccgcga
     4201 aaatgcgccg gcaggcgagc atgcccgatt cgatcacatc gagatcggcg agcagctcgc
     4261 gctgatcgag ttcgccgcgg gcgatgtcgg cgcggatctg ctgggccagc ggcagcaccg
     4321 agccgatggc attgttgacg tcgtgcgcga tccccctcga caggtccgcc agcgcgctct
     4381 tgcgctccgc ctgtaccacc tgctcggtga gtacctcggt gcggtggtgg aactgaaggg
     4441 cgagcgacag cagcggcgtg aagttctcca ccagctcgat ctcatacgcg ccgggaatat
     4501 cggcccgctg aaaagcaatc ttgagaatcc ccagcgaacc gtcgggagcc gggatcacgg
     4561 cgcagagcac cgcgttctct tcgggcgcgc tcgcgccccg cggacaggtg cagcagagcg
     4621 cggggatcag ccccggcgtc cggacggcgc cgaacaccgg ctgccagccg tggcccccct
     4681 ggcggtggag gtggatgccg ccgcggctta gctcgtcgcg gagctctgcc gccagcggta
     4741 ccgtcagccc gatgcggcgg ctctttgctt tcgtccaggc aatctgttcg gcgatgatct
     4801 ggagcggacc gtcgggaccg ttggtggcca ggaacgtggc cgagtggtcg tagcgggtca
     4861 gcgagcggat gccgtggagg atgaggtaga tgatgtcttt gggttcccgg cggttgacga
     4921 ctttctgttc gaggcgatgg cgcaggcggc ggatctgccg gtcgtcatac tgcgcgatcc
     4981 cttcccggac cgcctcggcg atgagaccga gcgcgcggag gtggaaggcc tccttgggat
     5041 ggggcaggcg cacggcgata gcgccccagg tcgccgcccg ccgctcgacc cgggccagca
     5101 ccacgtcgtc cggaatgcgc ggctcccgcc gcttgatgaa atcgttgagc aggccgacgt
     5161 tccattcggt cgaggtgcgc gtggtgaaga gcaggtcggc cgtgtgggcg gacgcgctca
     5221 acgtcgccac cgccccggcg tcggccgcga gaaactccac cgcccggcgc atgccgtaga
     5281 tgagcatctt gcggggctcg ccgtaatcgc gtagcgtgcc gcggagatcg aaaagtacct
     5341 tgtacaggtc ggcgtgcatc ttgtcgatag tgccgtcgaa catgacaact cctgcgtgcg
     5401 cggtgcggc
//
LOCUS       DAOOKS010000030         5344 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_18483_length_5344_cov_3.324825, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000030 DAOOKS010000000
VERSION     DAOOKS010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5344
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18483_length_5344_cov_3.324825"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..699
                     /locus_tag="PK112_00970"
     CDS             73..699
                     /locus_tag="PK112_00970"
                     /inference="COORDINATES: protein motif:HMM:NF017771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 family protein"
                     /protein_id="HPC10654.1"
                     /translation="MITRFGLTTTSQEAEMFATDSATTRRRIEDLRHQLLELRRQVRE
                     LRKTVPEEVIGREYVFAGPRGIRITLADLFGDRDELIIIHNMGRACRYCTLWADGLNG
                     LVPHLAGRASLVLVSPDPPEIQQAFAASRGWQFDLWSTGAEFLVDMKMADEKGSPWPG
                     ISAFVRRPDGTIVRTAFDYFGPGDDYCAVWHIWDMFPSGAGGWEPREG"
     gene            862..1674
                     /locus_tag="PK112_00975"
     CDS             862..1674
                     /locus_tag="PK112_00975"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HPC10655.1"
                     /translation="MADTLRLMGILAHPDDESLGMGGAFARYAREGVETFLITATRGE
                     RGRFRDLRGEARPPMPVVARAREGELRAAARVLGVREVHFLDYVDGDLDRAEPAEAIA
                     RIVGHLRRLNPQVVVTFAPDGAYGHPDHIAISQFTTAAIVAAADPAFPAPGAPPHAVA
                     KLYYLAWTRAKWDAYQAAFKQLATTVDGVERRATPWPDWAITTVVDTAAYWAVAWEAI
                     SCHETQLAIYTKLADLPPEYHRSLWGTQEFYRAFSLVNGGRKRETDLFEGLR"
     gene            1711..3219
                     /locus_tag="PK112_00980"
     CDS             1711..3219
                     /locus_tag="PK112_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013704703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPC10656.1"
                     /translation="MNSNDRTAARHTPIDLSPEEFRALGRDLVDRLAGLFASLRTRPV
                     TPEAAPGRIRERLGADRPLPDQGSDPAALLADAADLLFEHSLFNGHPRFWGYITSSPA
                     PIGALADLLAAAVNPNVGGWKLSPMASEIEAQTVRWIAELVGYPADCGGLLTSGGNAA
                     NFIGILAARAARAPWAVREEGMRGGRRMIIYASSETHTWLQKAADLFGFGTEAIAWIP
                     VDRFRRMDLARLEARVRTDRAEGHFPLAVVGSAGTVSTGAVDPLRDLAAFGRDQGLWF
                     HVDGAYGGFAAALPEASEDLHSLALADSLAIDPHKWLYAPLEAGCALVRDAEKLRHAF
                     SYHPPYYHFEEEAINYFDYGLQNSRGFRALKVWLALRQVGRAGYVQMIRDDIALARHL
                     FAHLAAYPDLERFTCDLSIATFRYVPADLRAGGGEEAREYLNELNENILERIERGGEA
                     FLSPAMIEGVYALRACVVNFRTTREDIEALPEIVVRVGRAADAARREKTLRR"
     gene            complement(3238..3990)
                     /locus_tag="PK112_00985"
     CDS             complement(3238..3990)
                     /locus_tag="PK112_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10657.1"
                     /translation="MSHPPFTVATLRDCPGLESEINRLASDLWPKFMLRDPVVEDNWR
                     LLYERYPEYQLFLLDGEDRRVIAVGNSCPLPWEGKPAELPDTGIDWILTELAARATFA
                     PKNQFALQIMVDRAQLGHGLSGQVVAAMAAIGRARGCRNLFAPVRPNRKGDYPLIPME
                     HYIRWTRDDGLPFDPWMRVHARLGAEIVGVCARSMYIPGSIAQWEAWTGLTFPGSGEY
                     VVPGGLAPVRVDRERDRAEYLEPNVWMRHRLT"
     gene            4149..4601
                     /locus_tag="PK112_00990"
     CDS             4149..4601
                     /locus_tag="PK112_00990"
                     /inference="COORDINATES: protein motif:HMM:NF020496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HPC10658.1"
                     /translation="MKPVTCRAELVQDDATSGCGITLPFDPKAQFGKVRAPVVATINR
                     YSFRTTVFSMGGCYWIPVNKSNRDAAGIAPGDRFKLRIALDTEPRAVTPPADLKEALA
                     GDPTAKTVWDKLSYTHRREYVEWIAQAKKPETRARRLQKTLERLKEPR"
     gene            4715..5200
                     /gene="bcp"
                     /locus_tag="PK112_00995"
     CDS             4715..5200
                     /gene="bcp"
                     /locus_tag="PK112_00995"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010871334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HPC10659.1"
                     /translation="MAVSSAVTVGRKAPAFALPNQQGDTARLSDYEGKWVVLYFYPKD
                     DTPGCTTEACDFTDNLSRLDKLNAVVLGVSPDSVESHARFVEKHNLKVTLLSDPEKKV
                     LQMYGAFGEKMMYGKATVGVIRSTYLIDPGGRIAYAWPNVKATGHAEKVAAKLAELAQ
                     R"
BASE COUNT          890 a         1852 c         1745 g          857 t
ORIGIN      
        1 gtgtcgccgc gttcactgac aggcgctctc aggcgagtgc gaccccacgt tgcgaaatgc
       61 acggaccggc ctgtgatcac gcggttcggc ctcactacta ccagtcagga ggccgagatg
      121 tttgcgaccg attcagcgac cacccgccgg cgtatcgagg atttgcgcca tcaactcctc
      181 gaactccggc gccaggtgcg cgagttgcgg aaaaccgtgc cggaggaggt gattggccgc
      241 gagtacgtct ttgcgggtcc gcgcggaatc cgcatcaccc tcgccgacct cttcggggac
      301 cgggacgagc tcatcatcat acacaacatg ggacgcgcct gccggtactg cacgctctgg
      361 gcggacggcc tcaacggtct cgtcccgcac ctggccggcc gggcgtctct cgtcctcgtg
      421 tcgccggatc cgcccgaaat ccagcaggcg ttcgccgcct cccgcgggtg gcagttcgac
      481 ctctggtcga ccggcgccga gttcctcgtc gacatgaaga tggccgacga gaagggttcc
      541 ccctggccgg ggatctccgc cttcgtccgg cggcccgacg ggaccatcgt gcgcaccgct
      601 ttcgattact tcggcccggg cgacgactac tgcgcggtct ggcacatctg ggacatgttc
      661 ccttccggcg ccgggggatg ggagccgcgc gagggctgaa cgggagggcc gccccgccaa
      721 cgcgccgtgc gcagccgcgg ccgggagcca tgaattcggc gcggaccgca acaatcctct
      781 tgtgccggcg tatctaatag actaagttgg tctattatcc tgccggcggc cggcaggggc
      841 agagaggaga ccgcgaccac ggtggcggac acgctgcgac tcatgggcat cctggcgcac
      901 ccggacgacg aatcgctcgg catgggcgga gccttcgccc gctacgcccg cgagggggtt
      961 gaaacctttc tcatcaccgc cacccggggc gagcgcggcc ggtttcgtga tctccgcggc
     1021 gaggcccgcc cgccgatgcc ggtcgtggcc cgcgcgcgcg agggagagct ccgcgcggcg
     1081 gcccgcgttc tgggcgtccg ggaggtccac ttcctggact atgtcgacgg cgacctcgac
     1141 cgggccgaac cggccgaggc gatcgcccgg atcgtcggcc acctgcggcg gctgaacccg
     1201 caggtggtgg tgacgttcgc gcccgacggc gcctacggcc acccggacca cattgcgatt
     1261 tcgcagttca ccaccgccgc catcgtcgcc gccgccgatc cggccttccc cgctcccggc
     1321 gcgcctcccc acgcagtcgc caagctgtac tacctggcct ggaccagggc caagtgggat
     1381 gcgtatcagg cggcgttcaa gcagctcgcg acgacggtcg acggggttga gcgccgggcg
     1441 actccctggc ccgactgggc gatcaccacg gtcgtcgaca ccgccgccta ctgggcggtc
     1501 gcgtgggagg ccatctcctg ccacgagacc cagctggcga tctacaccaa gctggccgac
     1561 ctgccccccg agtaccaccg ctccctctgg ggcacgcagg aattctaccg cgctttcagc
     1621 ctcgttaacg gcggccggaa gcgcgagacc gacttgttcg agggactgcg ctgaccgaca
     1681 cccgaaacgt ccgctacctg ggagagaccc gtgaacagca atgaccgcac tgccgcccgc
     1741 catacgccga tcgatctgtc gccggaggaa ttccgggcgc tcggccgcga cctcgtggac
     1801 cgccttgccg ggctgttcgc ctcgctgcgc acccgtccgg tgacgcccga ggcggcgccg
     1861 ggccgcatcc gcgagcgcct cggcgccgac cgcccgctgc ccgatcaggg atccgacccg
     1921 gccgcgctgc tggccgacgc cgccgacctg ttgtttgagc actctctgtt caacggccac
     1981 ccccgcttct ggggatacat cacctcctcg cccgcgccga tcggcgcgct cgccgacctg
     2041 ctcgcggcgg cggtgaatcc gaatgtcggc gggtggaaac tgtcgccgat ggcctccgag
     2101 atcgaggccc agaccgtccg ctggatcgcc gaactggtcg ggtaccccgc cgactgcggc
     2161 ggcctgctca ccagcggcgg caacgccgcc aacttcatcg gcatcctcgc cgcccgcgcc
     2221 gcgcgcgcgc cctgggccgt gcgcgaggag gggatgcgcg ggggccggcg catgatcatc
     2281 tacgcctcct ccgagacgca cacctggctg cagaaggccg ccgacctgtt cggattcggc
     2341 accgaggcga tcgcctggat cccggtcgac cgtttccggc ggatggatct cgcccgcctt
     2401 gaggcgcggg tgcggaccga ccgggccgag ggacactttc cgctggcggt cgtgggctcg
     2461 gcggggacgg tgagcaccgg ggcggtcgac ccgctccgcg acctcgctgc gttcggccgc
     2521 gaccaggggc tgtggttcca tgtcgacggc gcctacggcg ggttcgcggc cgcgctcccg
     2581 gaagcgtccg aggatctgca cagcctcgcc ctggccgatt cgctcgcgat cgatccgcac
     2641 aagtggctct atgcgccgct ggaggccggc tgcgcgctgg tgagggatgc cgagaagctg
     2701 cgccacgcct tctcctacca cccgccgtac taccatttcg aggaggaagc gatcaactac
     2761 ttcgactacg gcctgcagaa ttcccgcggt ttccgcgcgc tcaaagtctg gctggccctc
     2821 cgccaggtcg gtcgggcggg ctatgtgcag atgatccgcg acgacatcgc cctcgcccgc
     2881 cacctcttcg cgcatctcgc cgcgtatccg gacctggagc gtttcacctg cgatcttagc
     2941 atcgccactt ttcgctacgt gccggcggac ctgcgggccg gcggcgggga ggaggcccgg
     3001 gagtacctga atgaactgaa cgaaaatatc cttgagcgca ttgagcgcgg gggagaggcc
     3061 ttcctctcgc cggcgatgat cgagggcgtc tatgcgctgc gcgcctgcgt ggtgaatttt
     3121 cgcaccacgc gcgaggacat cgaggcgctc ccggaaatcg tcgtgcgggt cgggcgcgcg
     3181 gcggacgcgg cgcggcgcga aaagacactc cgccggtagc gggccgccgg cgctccctca
     3241 ggtaagccgg tggcgcatcc agacattcgg ctcaaggtac tcggcccggt cccgctcgcg
     3301 gtccacccgg accggcgcca gcccgccggg gacaacgtac tcgccggaac cggggaaggt
     3361 cagccccgtc cacgcttccc actgggcgat cgagccgggg atgtacatcg agcgcgcgca
     3421 gacgcccacg atctccgcgc ccaggcgggc gtgcacacgc atccacggat cgaacggcag
     3481 tccgtcatcg cgcgtccacc ggatgtaatg ctccatgggg atcagcgggt agtcgccttt
     3541 gcggttgggc cgcaccgggg cgaacaggtt acggcagccg cgggcccgcc cgatcgccgc
     3601 catggccgcc accacctgtc cgctcaggcc atgcccgagc tgcgcacggt cgaccatgat
     3661 ctgcagggcg aactggttct ttggtgcaaa cgtcgcccgc gccgccagct ccgtgagtat
     3721 ccagtcgatc ccggtgtccg gaagctcggc cggttttccc tcccatggca gggggcagga
     3781 attgccgacc gcgatcaccc gccgatcttc gccgtcgagc aggaagagct ggtattccgg
     3841 gtagcgttcg tagagcaggc gccagttgtc ctcgacgacc ggatcccgga gcatgaattt
     3901 cggccacaag tccgatgcca gccggttgat ttccgattcc aagccgggac agtcgcggag
     3961 cgtggccacg gtgaacggcg gatgagacat gcaatgcttc ctctacacgg ctttggggcc
     4021 gaactttgcc ggccagtata gcacgcgcgc gggaaaaagg ccagccgaat caggccggcg
     4081 caattgacaa cttgccccgc cgcgacaaac ggcgtatact gaaccgcacc tgcaaccaag
     4141 gggtcgctat gaaaccggtc acctgccggg ccgaattggt ccaggatgac gccaccagcg
     4201 gctgcggaat cacgctgccg ttcgacccga aagcacaatt cggcaaagtt cgggcgcccg
     4261 tggtcgccac gatcaaccgg tattcattcc gcacgaccgt tttctccatg ggcggctgct
     4321 actggattcc ggttaataaa tccaaccgtg acgcggccgg cattgcgccg ggagaccggt
     4381 tcaagctccg aatcgcactc gatactgaac cccgcgccgt tactccgccg gcggacttga
     4441 aggaagccct ggccggcgat cccacggcca aaaccgtttg ggacaaactc agctataccc
     4501 accgccgcga gtacgtcgag tggatcgcgc aggccaaaaa gccggagacc cgggcgcgcc
     4561 gcctgcagaa aacgctcgaa aggctgaaag aacctcggtg acggcgctgg cggcagagcg
     4621 ctcgcccgat ccgcccggga gcaaaggtgg cttcgcggcg gttgtactca ttgatcgccg
     4681 aataaggagc tttcttcaat aaggagtttt caccatggcg gtatcaagcg cggtgacagt
     4741 cggcaggaaa gccccggcct tcgccctgcc caaccagcag ggcgatacag ctcggctctc
     4801 ggattacgag gggaaatggg tggtcctcta cttctacccc aaggacgata cgccgggatg
     4861 tacgaccgaa gcctgcgact tcaccgataa tctgagccgg ttggacaagc tcaatgccgt
     4921 cgtgctcggc gtctcgcccg attcggtcga gagccatgcg cggttcgtgg agaaacacaa
     4981 cctgaaggtc accctccttt cggatcccga gaagaaggtg ctgcagatgt acggcgcctt
     5041 cggcgagaag atgatgtacg gcaaggcgac ggtcggggtg attcgctcca cctatctgat
     5101 cgatccgggc ggccgcatcg cctacgcttg gcctaacgtg aaagcgaccg gccatgctga
     5161 gaaagtcgcc gcgaaactgg cggagctggc gcagcgatga aaaagggacg cacccccgtg
     5221 ccgccggtgg cgttgctact gacaatcgag accaaccggc ttgcttcgac gtcccggccg
     5281 gagttgcaag gtgaaaccac atgcgcctga cggacatacg atgatcgccc gtcgttctgc
     5341 agtt
//
LOCUS       DAOOKS010000031         5339 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_18511_length_5339_cov_4.082324, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000031 DAOOKS010000000
VERSION     DAOOKS010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18511_length_5339_cov_4.082324"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1370
                     /locus_tag="PK112_01000"
     CDS             <1..1370
                     /locus_tag="PK112_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10660.1"
                     /translation="TGGAPSGATAAAPPDTAAADTARADSAGVVKKTGVYGGEYVFVS
                     EGRRDPLDSVLEEVSPDSIRALREGQVEPEKFDERPAAASDGDYAVKKYKVKFTPDYV
                     GGGVAYDTYFGLRGQTYFIFSDYLGNHQFYVATELVNTIDQSFIQAYYFNSVHRTSFG
                     GGVFHTKNFYLDANDYLFSDRFYGIQLFTTYPFSTFARMQLSGAQIFIDREYHDQYDL
                     RPNRNSKVTTGELAYVADNIVWGITGPVNGTRARLAVEAGVNLFDANDIAFQAASFDY
                     RRYWHFRKTYSMALRLTGGASFGKTPKLYFLGGTTNWIGNRTVDAKVYDVENLYFLDV
                     VTPLRGVEYYELSGNRYGLVNWEFRFPLIDYFVMRFPLGLALTNIQGVIFTDFGAAWT
                     DSDFKFGTSENGHRRLADPKAGFGFGMRANLGFVVLRYDLAWSTDFAEVSARSRSYLS
                     LGADF"
     gene            complement(1572..4124)
                     /locus_tag="PK112_01005"
     CDS             complement(1572..4124)
                     /locus_tag="PK112_01005"
                     /inference="COORDINATES: protein motif:HMM:NF025038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPC10661.1"
                     /translation="MPPKTKRTRKHTELTARQLDYSVNFRPQGVKSSDDVKPAVGVIG
                     QDRAIEAIRTGLNMTSPGYNIFVTGSVGTGRTDATLNLLEQFTRAKEPKLPDICYVNN
                     FKNEDCPRVLTFRAGQGSRFRKDMSYLIQSLRKVVPKVFMSEDYKDRQSRIIREYENR
                     QKELIHAFEERLSNVGFVMVQIQSGLGTRNEIQPLIEDNPVSLEQLERQARDGKFPLS
                     RLDQLRRTWDSLRREFDTITIEAKKLTGKMEDALEKLNHSMVAPLIADKVSLLKRRYP
                     GAKVVEYLDEVAEALQSDLERFRESQARRGEDEAPPYRKREPFEEFSVNLILDNAGAR
                     RVPVVFEKSPSYKNLFGSLERVVDRFGYWRTDFTRIFGGSLLRAAGGYLVLDALDALV
                     EPGVWTHLKRSLRNGEIEIAAYDPFYMMAGSGIKPEPIPIDVKVVLIGEPRVHQLLWH
                     LDEDFKKIFKIKAEFDTEVTADRSNFKRYFEFLRKVVDEESLPPFDLSGMQAVVEYGM
                     RLSGRRDKLSSRFSAISDIIREAAYCARQRGAAAANRDDVHCAITLKRRRVNLTEEKI
                     QEMFDQNVLMVDTTGAAVGRINGLSVYHIGDYSFGRPSRITASTSLGRSGIINIEREA
                     DLSGPIHNKGVLVMSGYLRQLFAQDKPLAVSASITFEQAYSGVDGDSASSAEVYAILS
                     SLGGLPIRQGIAVTGSVNQQGEIQPIGGVNQKVEGWFDVCAARGLTGDQGVIIPHQNV
                     NDLLIRPDVIAAVKARRFHIWPIRTIAEAIEILTGLPAGKRGANGRFPKGTVFGIVDE
                     KLMEMALTLQNFGRDDESSNRTDSAGKKRAGGAKESPRKRGRRRAAHPRRRT"
     gene            4596..4667
                     /locus_tag="PK112_01010"
     tRNA            4596..4667
                     /locus_tag="PK112_01010"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4629..4631,aa:Glu,seq:ctc)
     gene            complement(4760..>5339)
                     /locus_tag="PK112_01015"
     CDS             complement(4760..>5339)
                     /locus_tag="PK112_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10662.1"
                     /translation="GSIGILSLQASRRKKRARLGRTAAEIVPAFDHWTNGLWESGAGH
                     YSFAAVRRAPELNLLYPAEDRRIHRLQVALDGAPVGWAVVLHTLMKNHNYFGNMHVGT
                     IADCFAAPEQADAVIAAATDYLEERDVDLIVSNQMHPWWCSALANGGFLSGPSNFILA
                     TAPALTRRLAAADPAWERLHINRGDGDGPIDL"
BASE COUNT          899 a         1584 c         1806 g         1050 t
ORIGIN      
        1 caaccggagg cgcgccctcc ggcgccaccg ccgccgcccc gcccgacacg gcggccgccg
       61 acacggcccg ggccgacagc gccggcgtgg tgaagaagac cggcgtgtac ggcggcgagt
      121 acgtgttcgt cagcgaggga cgccgcgacc cgctcgattc ggttctcgag gaggtgtcgc
      181 ccgactcgat ccgggcgctg cgggaggggc aggtggaacc ggagaagttc gatgagcggc
      241 cggcggccgc ctccgacggc gactacgcgg tgaagaagta caaggtgaaa ttcacgcccg
      301 attatgtcgg cgggggagtg gcctacgaca cctatttcgg tctgcgcggt cagacctatt
      361 tcattttctc cgactacctc ggcaaccacc agttctatgt cgccaccgaa ctggtgaaca
      421 cgatcgacca gtcgttcatt caggcgtact acttcaacag cgtccaccgc acgagtttcg
      481 gcggcggcgt tttccacacc aagaatttct acctcgacgc caacgactac ctcttctcgg
      541 accggttcta cgggatccag ctgttcacga cctacccgtt ttccaccttc gcccggatgc
      601 agctctcggg agcgcagatc ttcatcgacc gggagtacca cgaccagtat gatctgcgcc
      661 cgaaccgcaa ttcgaaagtg accaccgggg agctggccta cgtggccgac aacatcgtgt
      721 gggggatcac cggtccggtg aacgggacgc gggcgcgcct ggcggtcgag gcgggcgtca
      781 atctcttcga tgcaaacgac atcgccttcc aggcggcgag cttcgactac cgccgctact
      841 ggcacttccg gaagacctat tcgatggcgc tgcggctgac cggcggagcg tcgttcggaa
      901 agacgcccaa gctgtatttc ctcgggggga cgaccaactg gatcgggaac cggacggtgg
      961 atgcgaaggt gtacgatgtc gagaacctgt atttcctcga cgtggtgacg ccgctgcggg
     1021 gggtggagta ctacgagctc tcgggcaacc gctacgggct ggtcaactgg gagttccgct
     1081 tcccgctcat cgattatttc gtcatgcgtt tcccgctcgg gctggcgctg acgaacatcc
     1141 agggcgtgat cttcaccgac ttcggcgcgg cgtggacgga cagcgatttc aagttcggca
     1201 cgagcgagaa cggccaccgt cggctggcgg atccgaaagc ggggttcgga ttcgggatgc
     1261 gggcgaatct cggattcgtg gtgctccgct atgatctggc ttggtcgacc gatttcgcgg
     1321 aggtgtcggc gcgctcgcgc tcatacctct cgctgggtgc ggatttctag cggtcccgat
     1381 cggacgggaa tgaaaaacgc ggccgtggtc cggccgcgtt ttcgttgttg ctcttttagc
     1441 attgcgcttc gagcggtgtc tgtcgcgcgt gtgacagaca cctgaataga agggcaatca
     1501 caaattttgt caagggaaaa agtggcctgc gggtgcggcg ccgcgcgcgc ggcgccgccg
     1561 gatcccccgc gctacgtccg ccgccgggga tgagccgctc tgcgccgccc gcgcttccgc
     1621 ggcgactcct tcgcgccgcc ggccctcttc ttgccggcgc tgtcggtacg gttggaggac
     1681 tcgtcgtcgc ggccgaaatt ctggagcgta agcgccatct ccatcaactt ctcgtcgacg
     1741 atgccgaaca cggtcccttt cgggaaccgg ccgttcgccc cgcgcttgcc ggcggggaga
     1801 ccggtgagaa tctcgatcgc ctcggcgatc gtcctgatcg gccagatgtg gaagcgccga
     1861 gctttcaccg ccgcgatcac gtcgggccgg atgagaagat cgttgacatt ctggtggggg
     1921 atgatgacgc cctggtcgcc ggtgaggccg cgggcggcgc acacgtcgaa ccagccttcc
     1981 actttctgat tcacgccgcc gatcggctgg atctccccct gctggttgac ggaaccggtg
     2041 acggcgatcc cctggcggat aggcaaaccg ccgagcgagg agaggatggc gtacacctcg
     2101 gccgaggagg cgctgtcgcc gtccacgccg ctgtaggcct gctcgaacgt gatggaggcc
     2161 gagacggcga gcggtttgtc ctgtgcgaag agctggcgga ggtagcccga catgacgagc
     2221 acgcccttgt tgtggatcgg cccggagagg tcggcctcgc gttcgatgtt gatgatgccg
     2281 gagcggccga gcgaggtgct ggcggtgatg cggctgggcc ggccgaacga gtagtcgccg
     2341 atgtggtaga ccgacagacc gttgatccgc ccgaccgcgg cgccggtggt gtccaccatg
     2401 agcacattct ggtcgaacat ctcctggatc ttctcttcgg tcaggttgac ccgccggcgt
     2461 ttgagcgtga tcgcgcagtg gacgtcgtcg cggttggcgg cggcggcccc gcgctggcgg
     2521 gcgcagtagg cggcctcgcg gataatgtcg gagatggcgg agaagcgcga cgagagcttg
     2581 tcgcgccgtc cggagaggcg catgccgtac tccaccacgg cctgcatgcc cgagaggtcg
     2641 aaggggggga gcgactcctc gtcgaccacc ttgcgcagga actcgaaata ccgtttgaaa
     2701 ttgctgcggt cggcggtcac ctcggtgtcg aactcggcct tgatcttgaa aatcttcttg
     2761 aaatcctcgt cgagatgcca gaggagctgg tggacgcggg gctcgccgat gaggacgact
     2821 ttgacgtcga tcgggatggg ctcgggcttg atccccgagc cggccatcat gtagaacgga
     2881 tcgtaggcgg cgatctcgat ctcgccgttg cggagggagc gcttgaggtg ggtccacacg
     2941 cccggttcga cgagggcgtc gagggcgtcg agcaccaggt agccgccggc ggcgcgcagg
     3001 agcgagccgc cgaaaatccg ggtgaagtcg gtgcgccagt agccgaaccg gtcgacgacg
     3061 cgctcgaggg agccgaagag gttcttgtag gagggggact tttcgaagac caccggcacc
     3121 cgccgggccc cggcgttgtc gaggatgagg ttgacggaga attcctcaaa cggctctcgc
     3181 ttgcggtagg ggggcgcctc gtcctcgccc cgccgcgcct gcgactcgcg gaaccgctcc
     3241 aggtccgact ggagcgcctc cgccacctcg tcgaggtact ccacgacctt ggccccgggg
     3301 tagcggcgct tcagcaggct cactttgtcg gcgatcagcg gcgcgaccat cgagtggttg
     3361 agcttctcaa gcgcgtcctc catcttgccg gtcagtttct tggcctcgat cgtgatcgtg
     3421 tcgaactcgc ggcggaggct gtcccaggtc cggcggagct ggtcgagccg cgacagcggg
     3481 aacttcccgt cccgcgcctg gcgctcgagc tgctccagcg agaccgggtt gtcctcgatc
     3541 agcggctgga tctcgttgcg cgtccccagc cccgactgga tctgaaccat gacgaagccg
     3601 acgttgctga ggcgctcctc aaacgcgtgg atcagttcct tctggcggtt ctcgtactcc
     3661 cggatgatgc ggctctggcg gtccttgtag tcctcggaca tgaatacctt ggggacgacc
     3721 ttgcgcagcg actggatcag gtagctcatg tccttgcgga accggctgcc ctgcccggcg
     3781 cggaaagtga gcacccgggg acagtcttca ttcttgaagt tgttgacgta gcagatatcg
     3841 ggcagcttgg gctccttcgc gcgcgtgaac tgctcgagga ggttgagagt cgcatcggtg
     3901 cggccggtgc cgacgctgcc ggtgacgaag atgttgtaac ccggcgaggt catgttcagg
     3961 ccggtgcgga tcgcttcaat cgcacgatcc tgcccgatca cgccgaccgc gggcttcaca
     4021 tcatcggacg acttcactcc ctggggccgg aagttgacgg agtaatcgag ttgccgggcg
     4081 gtcagttcgg tgtgtttgcg ggtccgcttg gtcttcggcg gcatgatcca aatcctccac
     4141 gggtatgaga gtttactacc tcaatataga attgaggggt gttgaaacaa cctcaaatgc
     4201 gacgtcgctg gtccagtcac atagtacgat gtgcccgcgc ggttggatga acggtcatgg
     4261 ggagacaaat atccggttat gatctaacga cttgagttgt gcgaccggtc attgctgtgg
     4321 agaattcctg tagttggctc atagttgtct gccacaatga cacatggtgc tgccatttga
     4381 gcgagtgtgt gattggtgtg tgatgcggtg gcgggtggaa aataggcaaa aaaacttgtc
     4441 aaatttcgcc gctgactaat tagttcggcc ccttggtatt acacgagatg attcttgtaa
     4501 ttcgttgtcc ggcaatggag tcacttcact tgacaggggt tttagatttt ctatattctg
     4561 agccgttgct tttttagcat tgcgcgttga gttttggcgg tatcgtctag gggttaggac
     4621 gtgtggttct cagccatgaa accggggttc gaatccccgt accgctaaga gtcaggctgt
     4681 gaaggggtca ggagagtggt cacctgaccc cttcggctat taatcgagca ctccactcgc
     4741 ggtccttcgc tgtcccctgc tacaggtcga tcggtccgtc gccgtcgccc cgattgatat
     4801 ggagtcgttc ccatgcggga tcggccgcgg cgaggcgccg ggtgagggcc ggcgcggtgg
     4861 cgaggatgaa attcgacggc ccggagagga agccgccgtt ggcgagggcc gagcaccacc
     4921 acgggtgcat ctggttggag acgatcaggt cgacatcgcg ctcctcgagg tagtcggtgg
     4981 cggcggcgat cacggcgtcg gcctgctcgg gcgcggcgaa gcagtcggcg atcgtgccga
     5041 cgtgcatgtt gccgaagtag ttgtggtttt tcataagggt gtggagcacg accgcccagc
     5101 cgaccggagc accgtcgaga gcgacctgca ggcggtggat gcgccggtct tcggccggat
     5161 acagcaggtt cagttccgga gcgcggcgga cggcggcgaa ggagtagtgg cccgcgccgc
     5221 tctcccacag cccgttggtc cagtgatcga acgcggggac aatctccgcg gcggtccggc
     5281 cgaggcgcgc acgcttcttc ctccgcgagg cctgcagcga gaggatcccg atcgagccg
//
LOCUS       DAOOKS010000032         5316 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_18621_length_5316_cov_3.599886, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000032 DAOOKS010000000
VERSION     DAOOKS010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5316
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18621_length_5316_cov_3.599886"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="PK112_01020"
     CDS             complement(<1..502)
                     /locus_tag="PK112_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10663.1"
                     /translation="MRLRRLDNLDLPPALAGRWAADRLPPFDELGGYLIGRQGKTRCA
                     LAETECFLRGEPSPVTRLELRLLPGPDTPPLRAAGSGRFTVGLHRSEIGTLPQIDRLA
                     ARLATRAGVRIWPTTLSLREHAGYRYRPLLDRTAPGGGYTLRQPDATGTAHLVFADRD
                     AVHRFRL"
     gene            complement(545..1249)
                     /locus_tag="PK112_01025"
     CDS             complement(545..1249)
                     /locus_tag="PK112_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10664.1"
                     /translation="MDTTLGRATAHLAEPDDRRGAGDLIALINRNINPPAPVTADAAV
                     IRAMYVVSDEINSFGGCFPSDEHAGLCRLLVDSPVMVGHRRDRLPIARNFHAEEVVRD
                     GRPWVKCWFYWLRAADGAETLRENIDGGIYRECSISFTFLFPECSICGKDIRACTHQP
                     FARYADGDGEAVCHFRYRGIERVLETSLVYRGALPGTRLTKDLRDRHPVRIPDETLIE
                     APTELPAAERYLVAPA"
     gene            1519..1800
                     /locus_tag="PK112_01030"
     CDS             1519..1800
                     /locus_tag="PK112_01030"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HPC10665.1"
                     /translation="MKVSHDTHSNPMKWVIAVIIFVIVLTVAFDDVFGVWIPQSDNEE
                     SVGAVESPDVHGDAKVYSDEPPPQVPEPGTLILLGAGLAAMRLLRPKKA"
     gene            complement(1984..2694)
                     /locus_tag="PK112_01035"
     CDS             complement(1984..2694)
                     /locus_tag="PK112_01035"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HPC10666.1"
                     /translation="MTSVEALINRQFMRWEREQLRRQEQEPPAAAVPPIITVSRQTGS
                     RGSYFASRLALALDYQRIHREVIDAVCASSGYCRRIVESLDERYRSNLELLIESALSG
                     TGIDRSDFTRQLYRVVLSIARLGGVVLIGRGANFILGPRHGFHVRFIAPRERRVRNLV
                     AYKGTTMIEASEMIDLSDRRRREFVRKIFGAEIDDPSYYDMIINADCIDIEELVEAVA
                     IAYRGKCSKLANLGNDIP"
     gene            2870..3343
                     /locus_tag="PK112_01040"
     CDS             2870..3343
                     /locus_tag="PK112_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10667.1"
                     /translation="MKLLRHTSALRPLLVGVLLALTALPAAAADVDSTKTVDHSGDVY
                     IIETRHRLFPDFLQVDTVGFDQPFFIGEEEWKARIILFNPHLGITTEGKGLQMSDTLY
                     NPAARIRVEQADTLVQESWAFHYVDAPHYRRTDLLGFKLLDFKVAAQYIPAPDKK"
     gene            3361..>5316
                     /locus_tag="PK112_01045"
     CDS             3361..>5316
                     /locus_tag="PK112_01045"
                     /inference="COORDINATES: protein motif:HMM:NF025890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HPC10668.1"
                     /translation="MKTFSRAAFLIVPLLLGLVLAPAASAVKTCYDCHQKQKQLYTSR
                     KFIHQPTKEENCESCHKRHGFAQTLVLTATTNDLCYSCHADLKAEFDKASVHFPVSDG
                     KCWDCHDPHASDKKGLLRAAPEGLDDPSACFMCHKAELDSSLHAAVPHPPFNELECTR
                     CHAPHASEHTGLLTAPANDLCGSCHSPADSRMTAAHGARHVAGLACTECHSGHAAGRK
                     GLLSDRTHEPFASGDCETCHSLPDDRGRVAFAEGVSPSDGCVTCHSDVAEKANLAHPH
                     AAMTGDNCTDCHRPHSSRFGSLLTAPQEELCGQCHSEVLTGKGLTPHLPAVLGQCSSC
                     HEVHGSANAALLARSEADLCLSCHSDFEAARDSAATVHAAADDCRQCHASHEGKTAAI
                     LRKDPSELCADCHAVDDAALGALSGHQPYLTSDCAACHSPHYSTAPHLVRGEGADACV
                     GCHADVQGRLGMTVPHPPAADDCRSCHVPHYGANAPHLLAAHEKDVCATCHDYDDLKV
                     TAAYVHAPARAGDCSGCHNPHGAMQENLVTGRMQRAVVDGRVVSQLPRLTGLSSDLCY
                     TCHDDLAEKFRRPGVHAPVAAGNCDACHAPHGSANPGFVTAPAPELCAACHAVDAGLQ
                     AAHDGFALDSANCLDCHNPHISEQPKLV"
BASE COUNT          915 a         1845 c         1665 g          891 t
ORIGIN      
        1 ggagacgaaa gcgatgcacc gcatcgcggt cggcgaacac caggtgcgct gtgccggttg
       61 cgtcgggttg ccgcagggtg tagccgcccc ccggggcggt tcggtcaaga agcggccggt
      121 agcggtagcc ggcgtgttcg cgcagcgaga gagtggtcgg ccagatgcgg acaccggcgc
      181 gcgtggccag acgcgccgcc aggcgatcga tctgcggcag cgtgccgatc tcgcttcggt
      241 gcagaccgac tgtgaaccgc ccggaaccgg cggctctcag gggcggcgtg tccggtccgg
      301 ggaggagccg aagctcgaga cgggtcacgg gcgagggctc gccgcgcagg aagcactccg
      361 tctccgcaag cgcgcagcgc gtcttcccct gccgtccgat caggtagccg ccgagttcat
      421 cgaacggcgg caggcggtcg gcggcccagc ggccggcgag ggcgggaggg aggtcgagat
      481 tgtccaggcg gcgcagccgc aactcgcccg gcccgctgca cgccgcggcg accggaatcc
      541 cctcttacgc cggcgccacg agatagcgct ccgccgcggg cagctcggtc ggcgcctcga
      601 tcagcgtctc atcagggatt cgcacgggat gccggtcgcg gaggtccttg gtcagccggg
      661 tgccgggcag ggccccccgg tacacaagcg aggtctcgag cacccgctcg atcccgcggt
      721 agcggaaatg gcagaccgct tcaccgtcgc cgtccgcgta ccgggcaaag ggttggtgag
      781 tgcaggcgcg gatgtccttg ccgcagatcg agcattcggg gaagaggaag gtgaagctga
      841 tcgagcactc gcggtagatg ccgccgtcaa tgttctcgcg gagggtctcc gcgccgtcgg
      901 ccgcccgcag ccagtagaac cagcacttga cccacggtcg gccgtcccgc accacctcct
      961 cggcgtggaa attccgcgcg atggggaggc ggtcccggcg gtgtccgacc atgaccggcg
     1021 aatcgaccag cagccggcac agtcccgcgt gctcgtcgga ggggaagcag ccgccgaagc
     1081 tgttgatttc gtcggagacg acatacatcg cgcgaatgac ggcggcgtcg gcggtcaccg
     1141 gcgcgggggg gttgatgttg cggttaatca gggcgatgag atcgccggcg cctcgccggt
     1201 cgtccggttc ggccagatgg gcggtcgcgc ggcccagggt ggtgtccatg gcgatttctc
     1261 ctccggtttt ttgcctagac cgcgccggcg gcattctcca ccgatgaggg cgcgaatttg
     1321 tgcagaataa tgccgacctc gggccgaccc cggactatgg ggacaagtcc ataatcctgc
     1381 ccctaagctg ctctgcggct tggactgcgg gcggccaggg gacggccgcg tcccgctgcg
     1441 gcacggtgct tgctctgatt aaagcggcag agattggagt gaaccgggag attcggtaaa
     1501 cgaaagaagg atgacgccat gaaagtttct cacgacacgc acagcaatcc aatgaaatgg
     1561 gtcatcgccg ttatcatatt cgtcatcgtg ctcacggtgg cttttgacga tgtcttcggc
     1621 gtctggattc ctcagagcga caacgaggag tcggtcggcg ccgtcgaatc gcccgacgtg
     1681 catggagacg ccaaggtcta cagcgatgaa cctccgccgc aggtccccga gccgggaacg
     1741 ctcatcctgc tgggcgccgg cctggcagcc atgcgcctgc tgcggcccaa aaaagcataa
     1801 gtccgttccc ctacctctac ccttccgagc ctcctgggtt tcctcccgct caggaggcgc
     1861 cttttttgtt ggatggccgg atcgacaaaa gccgccgcac tgtcgggggg actgaccgga
     1921 ccggggtctc gcggttcggc gaacgcctga tgggcgccgt gcccccgcct gcctcgccgg
     1981 gatttacgga atgtcgttgc ccaggttggc cagcttgctg cacttccccc ggtaggcgat
     2041 cgcgactgcc tccaccaact cctcgatgtc gatgcagtcg gcgttgatga tcatgtcgta
     2101 gtaactcgga tcatcgattt cggccccgaa aatcttgcgc acgaactcgc gccgccgccg
     2161 gtccgacagg tcgatcatct ccgatgcttc gatcatggtg gtccctttgt aggcgaccag
     2221 atttcggacc cgccgctcgc gcggcgcgat gaaccgcacg tggaatccgt gccgcggccc
     2281 cagaataaag ttggcccccc ggccgatcag cacaacccct cccagccggg ctatcgacag
     2341 caccacccgg tagagttggc gggtgaaatc cgagcggtcg atccccgtcc cgctcaacgc
     2401 cgactcgatc agcaactcca gatttgaccg gtacctctca tccagcgact ccacgattct
     2461 tcggcagtag cccgatgagg cgcagacggc atcgataacc tcgcgatgga tgcgctggta
     2521 gtccagggcc agcgccagcc gggaggcgaa gtaggacccg cgcgaccccg tctgccgcga
     2581 caccgtgata atcggcggca cggcggctgc cggaggttcc tgctcctgcc gtcgaagttg
     2641 ctcgcgctcc caccgcatga attgccgatt gataagcgct tctactgagg tcatggcgcc
     2701 acatctccct gctaggttat acgccaggat agaatatcgt acgaagtggt acaattaaaa
     2761 acgttgactc cggagctcgt caaatgccgc catgcggcag aatgcgcttg acaacaggac
     2821 aattttagtt acttttttca caaatttcga tacagaatgg aaatgtcgca tgaagcttct
     2881 tcgccatacg tccgcccttc gaccgcttct cgttggtgtt cttctggcct tgaccgccct
     2941 tcccgcggcg gcggccgatg tcgactcgac caaaaccgtc gatcattcgg gtgatgtcta
     3001 catcatcgag acgcgccacc ggctcttccc ggattttctc caggttgaca cggtagggtt
     3061 cgaccagccg tttttcatcg gggaggagga gtggaaagcc cgcatcatcc tgttcaatcc
     3121 gcatcttggg attacgaccg aggggaaggg tctgcagatg tccgacacgc tgtacaatcc
     3181 ggcggcacgg atccgcgttg agcaggcgga cacgctggta caggaaagct gggcgtttca
     3241 ttatgtcgat gcgccgcact accgccgcac cgacctgctc ggcttcaagt tgctcgactt
     3301 caaggtcgcc gcgcagtata ttcccgcacc cgacaagaaa taaggcggag tcacgtggca
     3361 atgaagacat tctcccgcgc cgcgttcctg atcgtaccgc tattgcttgg gctcgtcctg
     3421 gccccggcgg cctcggcggt caagacctgc tacgactgcc accagaaaca gaagcagctc
     3481 tataccagcc ggaaattcat ccaccagccc accaaagagg agaactgcga gtcctgccac
     3541 aaacgccacg gtttcgccca gacccttgtg ctgactgcga caaccaacga tctctgttac
     3601 tcctgccacg ccgatctgaa agccgaattc gacaaggcca gcgtccattt cccggtctcc
     3661 gacggcaagt gctgggactg ccacgatccg cacgcttccg acaagaaagg gctgctccgg
     3721 gcggcgccgg aggggctcga tgatccgagc gcctgtttca tgtgccacaa ggcggagctg
     3781 gactcgtcgc ttcacgccgc ggtgccccat ccgcccttca acgaactcga gtgcacccgc
     3841 tgccacgcgc cccatgcctc ggagcatact ggtctcttga ccgctccggc caacgacctc
     3901 tgcggatcgt gccattcccc ggccgacagc cggatgacgg cggcccacgg cgcccgccat
     3961 gtcgccggcc tcgcctgcac cgaatgccac tcgggccacg ccgccgggcg caagggactt
     4021 ctctccgacc ggacccacga gccgttcgcc tcgggcgact gcgagacctg ccacagcctc
     4081 cccgacgatc ggggccgggt ggccttcgcg gagggcgtgt cgcccagcga cggatgcgtc
     4141 acctgccact cggacgtggc cgagaaagcg aacctcgccc acccgcacgc cgccatgacc
     4201 ggcgacaact gcaccgactg ccaccgcccg cattcctccc gtttcgggag ccttctcacg
     4261 gcgccccagg aggaactgtg cggccaatgc cacagcgagg tgctcacggg caaggggctc
     4321 acgccgcacc tgccggcggt cctcggccag tgcagctcct gccacgaggt ccacggctcg
     4381 gccaacgccg cgctcctggc gcgctccgag gccgatctct gcctgtcctg tcacagcgat
     4441 ttcgaagccg cccgcgattc ggccgcgacc gtccacgccg ccgcggacga ctgccggcag
     4501 tgccacgcct cccacgaggg caagaccgcc gcgattctgc ggaaagatcc ctccgagctg
     4561 tgcgccgatt gccacgccgt cgacgacgcc gccctcggcg cgctctccgg ccaccagccc
     4621 tacctcacct ccgactgcgc cgcctgccac agtccgcact actcgacggc gccgcacctg
     4681 gtgcggggcg agggggcgga cgcctgcgtg ggctgccacg ccgacgtgca gggccggctg
     4741 ggcatgaccg tgccgcatcc cccggccgcc gacgactgcc ggagctgcca tgtcccgcac
     4801 tacggcgcca atgctcccca tctgctggcg gcgcacgaga aggacgtgtg cgcgacctgc
     4861 cacgactacg atgatctgaa agtaaccgcc gcgtatgtcc acgcgccggc tcgcgcgggc
     4921 gactgcagcg gctgccacaa cccccacggc gccatgcagg agaacctcgt gaccgggcgc
     4981 atgcagcggg ccgtggtcga cggccgcgtc gtctcgcagc tgccgcgcct gaccggcctc
     5041 agctccgacc tgtgttacac gtgccatgac gacctcgcgg agaaattccg ccggcccggc
     5101 gtgcacgctc cggtggccgc cgggaactgc gacgcctgcc atgcgccgca cggttccgcc
     5161 aatccgggat tcgtcaccgc ccccgcccct gagctgtgcg ccgcgtgcca tgcggtcgat
     5221 gccggcctgc aggcggctca cgacggtttc gcgctcgact ccgccaattg cctcgactgc
     5281 cacaaccccc acatctccga acagcccaaa ctcgtc
//
LOCUS       DAOOKS010000033         5170 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_19301_length_5170_cov_2.924536, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000033 DAOOKS010000000
VERSION     DAOOKS010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5170
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19301_length_5170_cov_2.924536"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="PK112_01050"
     CDS             complement(<1..324)
                     /locus_tag="PK112_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011457098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HPC10669.1"
                     /translation="MNSGSPNPTMDIVEAAAAAGNFTTLLAAAEAADLVDALKGAGPI
                     TLFAPTDDAFAKLPAGTVEALLADKDALTAILTYHVVPGAVTADQVVRLSSAETLNGA
                     SVTITL"
     gene            complement(538..696)
                     /locus_tag="PK112_01055"
                     /pseudo
     CDS             complement(538..696)
                     /locus_tag="PK112_01055"
                     /inference="COORDINATES: protein motif:HMM:NF015996.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
     gene            1480..1878
                     /locus_tag="PK112_01060"
     CDS             1480..1878
                     /locus_tag="PK112_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPC10670.1"
                     /translation="MDNRIIVSTILIAVFAMAVPGLADSTSQKAEKTYKIHGFLGESS
                     TVAAPNKTVTLLDADSNTALANTNSNFFGKYAFKKLPPGHYLVKVAEKVMEVYLIDKD
                     IRLDVDLSANDGTMNYAAGAAAQAAKPAEK"
     gene            1972..2253
                     /locus_tag="PK112_01065"
     CDS             1972..2253
                     /locus_tag="PK112_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10671.1"
                     /translation="MGGGGGSESQIAFCPDGSYFESYESGYYGSGQWGQAGQSRASGT
                     WSITGTIETGTITITYANGKQETVRYETTGESGCYKFGGRLYCYNGACD"
     gene            2619..3188
                     /locus_tag="PK112_01070"
     CDS             2619..3188
                     /locus_tag="PK112_01070"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPC10672.1"
                     /translation="MKRSHLSDQQLWESVLVGKARAWDELVSRYGPLVYAVCRQVGIS
                     ESDAADVFQHSWIALYENRFRLEDPTRIASWLLTTAKREALRYRQRQARLQSDDCLAE
                     RADERPLPDEELRRLELQARLELALRSIDRLCRDLLRAFFFAGEDQSYEQIAAAFHIA
                     PNSLGAKRRRCLDKVRAVLIRLGYLEERK"
     gene            complement(3418..3741)
                     /locus_tag="PK112_01075"
     CDS             complement(3418..3741)
                     /locus_tag="PK112_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10673.1"
                     /translation="MHDKAGGRARRPPLAYPEVIRIDHFCVGLYDKSVGGADALRDAR
                     PPGLFLFNPQLYRDPSRFEVQAAVVFDRRSAHADRRRRRPGIEYQTRGHLDQSGTRRR
                     TFPGD"
     gene            3777..>5170
                     /locus_tag="PK112_01080"
     CDS             3777..>5170
                     /locus_tag="PK112_01080"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPC10674.1"
                     /translation="MEANETDIAARRFLRTGSTGVHGESAVAAACEKLIQKAFRTSIR
                     RALGLAERFHARAQAHGGRVLGQTAWRSLGRMHHFSGRHAEARHCYRAARTLCGDDLL
                     IRGRIDRTLIDINMYLGNDRAAAAAGRAALRAFSALEAESDLAQTRVNLGNLYHRRDK
                     PVEADRLYRQAALFFARSGNKLALARTNYNRANVLVQLFQMDEAEKLYRDALAAYTES
                     GYELDACDTRYALAWLNLLTGRFHQALSDLAACEATYRTAGDPRGVALCLLDRAEVFL
                     QLGLYRDALDLAGRAGKRFRTLKLEYESGKAHLFAAYALTGLGRKDRARAAWARAEQG
                     FAALRHAGYLGTLHLLRTDLDQGSPGTRQTALRAARRCFDRAALFFWQAVCDLKAIEL
                     SGRTPVYLARLRRNPAAWCVPHLYAAWQVAEGDLHLVSGRRAAALTAWRKAADRLDLV
                     RSALPPMELRNQYAR"
BASE COUNT          972 a         1611 c         1660 g          927 t
ORIGIN      
        1 gagagtaata gtgacgctgg cgccgttgag agtctcggcg ctggacagcc tcacgacctg
       61 atccgcggtg accgcacccg gcaccacgtg gtaggtcagg atagccgtca aagcatcctt
      121 gtcggccagc aatgcttcga ccgtaccggc cgggagcttg gcaaaagcat cgtctgtggg
      181 ggcaaacagc gtgatcggtc ccgcaccctt gagcgcatcg acgagatcag cggcttcagc
      241 cgcggccaga agagtcgtga aattgccggc cgccgcggcc gcctcgacga tgtccatggt
      301 gggattcgga gaaccgctgt tcatcggatt ctcgtcgtcg ttgctgcagc cagtcatgcc
      361 gatcgccagg gcagccacaa gggccgtcat cagtgtaaac ttccgggtga acatcttttt
      421 gctccttact atactatgaa cgttttcttt tgttttcggt attcgtccgc atctgtcggt
      481 gttgcggccg gctaggccac ccaccggcgc tatggccttc cactgaacca cttgatagag
      541 agcccccaaa ccgaccacca tatacaccag ccggctcaag agggtggcgt cgccgagcag
      601 tgtggcgacc aggtcgaaat gcaggaggcc caccagaccc cagttgagtc ccccgactac
      661 catcagggcg acagcgatca cgtcgaatga tttcattcca gtactccttt ttgcgtattc
      721 tgttcattat ttgtctaagt tctgacttat gtaactccga agctggcgga tcggttcacg
      781 aaaaaggaga aaatggcgaa aaatatcata accaattgta atcacactag ttatgttata
      841 ttctcatggg ctatgtcagg ttcacgatga tcgccaaagt gtccatatta ttgaacagat
      901 acgagacggg tgaatcactc gacagcactg ccggtggcct gcggggagat cgccaaaggc
      961 agcagggccg aaaaacgcct gcgcggaggg ccaccgccgc gtgcgcgatt cgattgcccc
     1021 gccgttaggc acgccgtccg ttacagccat caccaggtat ctgagtagtg tcctcgtgtc
     1081 agataagaca agagcggggc gcggagtcga tccgagagta ctgcgacagc tcatcatgag
     1141 ttgtcttact cagaatgtca gggccggcga tcggagagtc acccggtccc cgccgacgcc
     1201 cggcgtcggc gacggaatgt ctatgcccga ttagggagtc gggggcttgt cgcgccccgg
     1261 tcgcaggccc tgccgcgtgg cccggagtct tgtcgatgtg agggacaccg caattcttgg
     1321 cgagactatc gtgcgagcta tcatcaggga gtgagcatgg agctccgcgg gaggttcgac
     1381 cgacttcaaa gtatcgtttg aaacgaggtc cgggatatga gtatcttccg gtatgcatga
     1441 acctcaccgc agtaccacca ctgtaaagga ggagtatcat tggacaatcg aataattgta
     1501 tcaacgattc ttatcgccgt cttcgccatg gctgttcccg gcctggccga ctcgacctcc
     1561 cagaaggcgg aaaagactta taagattcac gggtttctgg gggagtcatc gacggtggcg
     1621 gcgcccaaca agactgtcac gctgctcgat gccgacagca acacggcgct ggctaatacc
     1681 aacagcaact tcttcggaaa gtacgccttc aagaaactgc cgccagggca ctatctcgtc
     1741 aaagtagccg agaaagtcat ggaggtctat ctgatcgaca aagacattcg tctcgatgtc
     1801 gacttgagcg ccaatgacgg gaccatgaac tatgccgcag gggccgcggc gcaggccgcg
     1861 aagccggccg aaaagtaagg cgaggggact ccccccggcc ggccggaggg ggacaccgat
     1921 ctcgccaaac aattctccgg caagtactac agctacaccg gaggctcgac attgggcggc
     1981 gggggcggtt cggaaagtca gatcgctttc tgtcccgacg gcagctattt cgaaagttat
     2041 gagtcgggct actatggttc cgggcaatgg gggcaggcgg gccaaagccg cgcgagcggc
     2101 acctggtcaa tcaccggcac gatcgaaacc gggacgatca ccatcactta cgccaacgga
     2161 aaacaagaga cggtacgcta cgagacgacc ggtgagagcg gctgctacaa attcggcggc
     2221 cggttgtact gctacaacgg cgcctgtgat taggaggaga gcatctatta ttgtggatat
     2281 tccggcgtga ggttccagat ccgattgcgc cactcctcgg ttggcggaag cgtgctcgga
     2341 gagggtcggt tgccagagtg ccacgggagg caggtgagcc tgttcctgac acacggtgtg
     2401 atcagcgtct cgaccatccg gaccggttca tgaccggtga cgccggttat gtcggcaacg
     2461 tctgctcatc gaccgggtga ggttacccgc catcacaagg aacagcggag ctgagtgcgg
     2521 cgtgcagaag ttccgccggg cgacagccgg cggatttcgt ttgccttatc cggcatttga
     2581 gttactttga cggcagaact gcgcccccag ggagaaccat gaagcggtcg cacctgtccg
     2641 atcagcagct gtgggaaagc gtgctcgtgg gcaaagcccg ggcctgggac gagcttgtca
     2701 gccggtacgg gccgctcgtc tacgccgtgt gccgccaggt gggcatcagc gagtccgatg
     2761 ccgccgatgt attccaacac tcctggattg ctctctacga aaaccgcttc cgtctcgagg
     2821 acccgacccg catcgcctcg tggctgctga caaccgccaa acgggaggcc ctgcggtacc
     2881 ggcagagaca ggcgcggctg cagtccgatg actgcctggc cgaacgggcc gacgagcgac
     2941 cgctgccgga cgaagagctc cggcgcctcg agctccaggc ccggctggag ctcgccctgc
     3001 ggtcgatcga ccggctgtgc cgcgatctct tgcgggcgtt cttcttcgcc ggggaggatc
     3061 agtcgtacga gcaaatcgcc gccgccttcc acattgcccc gaactcgctg ggcgccaagc
     3121 ggcgccgttg tctggataag gtgcgcgccg tgctcatccg tctgggatat ctggaggaac
     3181 ggaaatgacc cagaccgcct ctgttctagc ggagaaggag tgatcatgag cccagagaag
     3241 cacctgactc ggaagcggct aatcgaaatt gcgcgcgcgg gcactccccc gtcggattct
     3301 cacttggccg cctgcgacgt gtgccgggag gcgtacgaga tgctgtcccg gtactactcc
     3361 ctgatcggcc gccctcccct tcccgcacca ccggccgact tcctgaggcg ggccaggtca
     3421 atcgcccggg aaggtgcgcc gtcgggtgcc cgactggtcg aggtggccgc gcgtctggta
     3481 ttcgattcct gggcggcgcc ggcggcggtc ggcgtgcgcg gaccggcggt cgaaaacgac
     3541 cgccgcctgc acttcgaagc ggctggggtc gcggtagagc tgcgggttga acagaaagag
     3601 accgggtggt cgtgcatcgc gcagtgcgtc ggcacctccg accgatttgt catagagacc
     3661 gacgcagaag tggtctatcc ggatgacctc ggggtatgcc agtggcgggc gccgcgcccg
     3721 cccgcccgcc ttgtcgtgca caccgccgca acccgcgtca ttgtctccag actccaatgg
     3781 aagcgaacga gacggacata gcggcgcggc ggttcctgcg cacgggatcg accggggttc
     3841 acggcgagag cgccgtggcc gccgcctgtg agaagctgat ccagaaggcg tttcgcacgt
     3901 cgattcgccg cgcgcttgga ctggccgagc ggtttcacgc ccgggcgcag gcgcacggcg
     3961 gacgggtact ggggcagacg gcctggcgct cgctcggacg gatgcaccac ttctccggcc
     4021 ggcacgccga ggcgcggcac tgctaccggg ccgcgcgcac gctctgcggc gacgacttgt
     4081 tgatccgcgg ccgcatcgac cgcacgctca tcgacatcaa catgtacctg ggcaatgacc
     4141 gggcggccgc cgccgccggc cgcgctgccc tccgcgcctt cagcgccctc gaggccgagt
     4201 ccgatctggc gcagacgcgc gtcaacctgg gcaatctcta ccaccgccgg gacaaaccgg
     4261 tcgaggccga ccggctctac cggcaggccg ccttgttttt cgcgcgaagc ggcaacaaac
     4321 tggccctggc ccgcaccaac tacaaccggg ccaacgtact cgtgcagctc ttccaaatgg
     4381 acgaggccga gaagctctac cgggacgccc tcgccgcgta caccgagtcg gggtacgaac
     4441 tcgatgcctg cgatacccgc tacgctctgg cctggctgaa tcttctgacc ggccgtttcc
     4501 accaggccct gtccgacctc gctgcatgcg aggcaacgta tcggaccgcg ggcgatcccc
     4561 gcggcgtggc gctctgcctg cttgaccggg cggaagtgtt tctccagttg gggctctatc
     4621 gagacgccct cgacctggcc ggccgggcgg ggaaacggtt tcggacgctg aagctcgaat
     4681 atgagtcggg gaaggcgcac ctgttcgcgg cctatgcgct gaccggcctc ggccgaaagg
     4741 accgggcccg ggcggcgtgg gccagagccg agcaggggtt tgccgccctt cggcatgccg
     4801 ggtacctggg aaccctgcac ctgctgcgga cggatctcga ccagggatcg ccggggacgc
     4861 ggcagaccgc gctgcgcgcg gcgcgccgat gcttcgaccg ggcggcgctg ttcttctggc
     4921 aggcggtgtg cgatctgaag gcgatcgaac tgagcggacg cacgccggtc tacctggcgc
     4981 gccttcgccg caatccggcc gcgtggtgcg tgccccatct ctacgccgcc tggcaggtgg
     5041 cggaggggga ccttcacctc gtctccgggc ggcgggcggc ggcgctcacg gcctggcgga
     5101 aagcggccga ccgcctcgat ctggtccgat cggcgctgcc gccgatggaa ttgcgcaacc
     5161 aatatgcgcg
//
LOCUS       DAOOKS010000034         5149 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_19419_length_5149_cov_4.110326, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000034 DAOOKS010000000
VERSION     DAOOKS010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5149
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19419_length_5149_cov_4.110326"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="PK112_01085"
     CDS             <1..283
                     /locus_tag="PK112_01085"
                     /inference="COORDINATES: protein motif:HMM:NF019998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HPC10675.1"
                     /translation="FARSELGAAPPAGELTAAVRPEKIRIEPEGRFAASVIGCEYLGD
                     QYVVTLKFGETVLTVAGVTREMRAGEPARFAIAPRDLLFFDRASGRRVG"
     gene            complement(349..471)
                     /locus_tag="PK112_01090"
     CDS             complement(349..471)
                     /locus_tag="PK112_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10676.1"
                     /translation="MFNLPAAAAVLHLFNFPLGAALGVYSFRALLDLPSRSLFR"
     gene            complement(533..2359)
                     /gene="pepF"
                     /locus_tag="PK112_01095"
     CDS             complement(533..2359)
                     /gene="pepF"
                     /locus_tag="PK112_01095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HPC10677.1"
                     /translation="MSCATGTDDQKTIPTRDQVEERYQWNLADLYLSDEAWDDDARRV
                     EELIRNAAGFAGRLHESPDTLYRCLKSRSDLGLAMFRLYQYAKLSQDIDNRVSKYQAM
                     TDRAAALSARGRAAFAFVEPELLAIDEQALRAMAARFPHTDEYDFYINELIRTRAHVR
                     TQEVEELLALSAQVTRGPETIFTMLDDADLSYPTVQDESGRDVQLTKQRYAKLMESSD
                     RRVRHDAYHAFYRPYREHLNTLGASLAAAVNADVFYARARRFDSCLAAALFGDNIPVS
                     VYRNLIETTEAHLEGMHHYLRVRQRLLKVEQLHTWDLACPLFPEADYEVAYDDAIAEI
                     LDALRPLGPTYLDVLRRGFASRWVDVFETAGKASGAFSWGSYGVHPFVLMNYNATVDN
                     MFTLAHEMGHALHSHLSNTHQPYPKAQYSIFVAEVASTLNEGLLLQHLLSKAQDKTTR
                     LYLLNRYIDNTMGTFFNQVLYAHFELAIHAQVEGGGALSPEWLTALWADLTRKYYGPD
                     LGDDPYFGLKWCRIPHFYMTFYVYQYATSYAASESILDNVLAGGPDSINRYLDLLAAG
                     GSDYPIELLRRASVDMSRPAAIESTLTRFADQVAELDRLSQE"
     gene            complement(2518..4194)
                     /locus_tag="PK112_01100"
     CDS             complement(2518..4194)
                     /locus_tag="PK112_01100"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HPC10678.1"
                     /translation="MRKSFLLRTTAGVLVFAAALLLAATVPAADKTAELDAYLSAACD
                     VLRFQGAVLVADDGVPVFVKGYGMADVAHGQPNTPETEFLIGSVTKQFTAAAILQLQE
                     QGTLSVHDPISKFLPDFPKATGDRITVHHLLTHTSGLVSYTDIDSLMARRGLDMTARE
                     IVASFKDLPLQFEPGAMYRYCNSGYFLLGLIIEAVSGQTYADYLAEHIFRPLGMTHTT
                     YPPNERTPNRAVGYVAPEDTLVPADPISMTLPYAAGALASTVGDLLIWDQALYGDRVL
                     SEDSRKQMYTPALENYGYGWMVAERFGRREISHGGGIDGFTSVIARYPDDRLTIIVLC
                     NNMSANAAGLGSTLAAIMFDQPYDRPVRKTPAAADPASFDALVGVYWVGDNSYRVIRR
                     DGDNLYSQRTGGPVFQVFPEAPDKFFFENDNSTTLVFIRDQAGAVVAHLIHQSGVDTR
                     CDRVTGPLADSLMAERAVVPIDPAVFARLVGDYELAPGFVLTFRARDGRFFSQATGQQ
                     EFEIFPAGETEYFLRVVDAQITFQLDPATGRATGLILHQGGRDMPAKKIK"
     gene            complement(4309..5142)
                     /locus_tag="PK112_01105"
     CDS             complement(4309..5142)
                     /locus_tag="PK112_01105"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HPC10679.1"
                     /translation="MQTIEEFRAMLNDAVGYITSQIAFTPRIGIILGTGLGKLVDGID
                     MVGTVSYEKIPHFPVSTVESHAGRLLFGRLRGKPVVCMQGRFHYYEGYSFPQIAFPVR
                     TLKALGIHTLIVSNAAGGLNPNFAPGDIMLIKDHINFFPGNPLIGPNDDTLGDRFPDM
                     YEVYHRRYQALARQVALELKIRLQEGVYVGLTGPCLETAAEYRMLRGFGADAVGMSTV
                     PEVLTAHHQRTKILAFSIITDMGLPDNMHPCSLADVIGAASATEPILRDLIAGCVEKM
                     E"
BASE COUNT          882 a         1436 c         1948 g          883 t
ORIGIN      
        1 gtttgcacgg agcgagctgg gggcggcgcc gccggcggga gaactcaccg cggcggtgcg
       61 gccggagaaa atccggatcg aaccggaggg gcgctttgcc gcgagcgtca tcggctgcga
      121 gtacctcggc gatcagtatg tcgtgacgct gaagttcggg gagacggtgc tcacggtggc
      181 gggcgtgacc cgggaaatgc gcgcgggcga gccggcgcgg ttcgccattg cgccccggga
      241 cctcctgttt ttcgatcggg cgagcgggcg gcgcgtcggg taaagcgggg ggccacggcc
      301 gcgctttccg ggggaagccg gccgatccgg cagccagggt tgcacagtct accggaacag
      361 ggagcgggac ggcaggtcaa gaagggcccg gaaggagtag acccccagag ccgcgccgag
      421 cgggaagttg aacaggtgga ggacggcggc cgcggcgggg aggttgaaca cgaagatgac
      481 tatgttcaga ataccacggc tttcacgtgc ttttccctcg ggcggcgcgc tcctattctt
      541 gcgagaggcg gtcaagctcc gcgacctggt cggcgaaacg ggtgagagtg gactcgatcg
      601 ccgcggggcg gctcatgtcg acactggcgc ggcgcaggag ctcaatcggg tagtcgctcc
      661 cgccggcggc cagcaggtcg aggtagcggt tgatcgagtc agggccgccg gccaggacgt
      721 tatccagaat cgactcggag gcggcgtagg aggtggcgta ctggtagacg tagaaggtca
      781 tgtagaagtg cgggatgcgg caccacttga ggccgaagta gggatcgtcg cccaggtccg
      841 ggccgtagta ttttcgggtg aggtcggccc acagcgccgt gagccactcg ggcgacaggg
      901 ctcccccgcc ctcaacctgt gcgtggatgg ccagctcgaa gtgggcgtag agaacctggt
      961 tgaagaaggt ccccatggtg ttgtcgatgt accggttgag caggtagagc cgggtggtct
     1021 tgtcctgggc cttcgacagc aggtgctgca ggagaagacc ttcgttcaga gtggaggcga
     1081 cttcggcgac gaagattgag tactgggctt tcgggtaggg ctggtgcgta ttggagaggt
     1141 gggagtgcag ggcatggccc atctcgtggg cgagcgtaaa catgttgtcg accgtggcgt
     1201 tgtagttcat gagcacgaac gggtgcacgc cgtagctccc ccagctgaac gcgccggagg
     1261 ctttgccggc ggtttcgaag acatccaccc agcgcgaggc gaacccccgg cggagaacgt
     1321 cgaggtaagt cgggccgagc ggccggaggg cgtcgagaat ctccgcgatc gcatcgtcgt
     1381 aggcgacctc gtagtcggcc tcgggaaaga gcgggcaggc gagatcccag gtgtggagct
     1441 gctccacttt gaggagacgc tgccggacgc gcaggtagtg gtgcatcccc tcgagatggg
     1501 cctcggtggt ctcgatgagg ttgcggtaga cggagacggg gatgttgtct ccgaagaggg
     1561 cggccgccag gcagctgtcg aaccggcggg cgcgggcgta gaacacgtcg gcgttgaccg
     1621 cggcggcgag cgacgccccg agcgtgttga ggtgctcccg gtaggggcgg tagaacgcat
     1681 ggtaggcatc gtgccgcacc cggcggtcgg acgattccat cagcttggcg tagcgctgct
     1741 tggtgagctg gacatcccgg ccggactcgt cctggacagt cgggtaggag aggtcggcgt
     1801 cgtccagcat ggtaaaaatt gtctccgggc cgcgagtgac ctgggccgag agggcgagga
     1861 gttcctccac ctcctgggtg cggacgtggg cgcgggtgcg gatgagctcg ttgatgtaga
     1921 agtcgtattc gtcggtgtgc gggaagcgcg cggccatcgc gcggagcgcc tgctcatcga
     1981 tcgccagcag ttcgggttcg acaaaggcga aagcggcgcg gccgcgggca gaaagggccg
     2041 cggcgcggtc ggtcatggcc tgatatttcg agacccggtt gtcgatatcc tggctcaact
     2101 tcgcatattg gtagagacgg aacatggcca gcccgaggtc ggagcggctc ttcagacacc
     2161 ggtagagcgt gtccggcgac tcgtggaggc gcccggcgaa cccggcggcg ttccggatga
     2221 gttcctcgac gcggcgggcg tcgtcatccc aggcctcgtc ggacagatag aggtcggcga
     2281 ggttccattg gtagcgttcc tcgacctggt cgcgggtggg gattgttttc tggtcatcgg
     2341 tgccggtcgc gcaggacatg gggggccttt cgtttggttg gttcgccatc gggcgcaata
     2401 tcgcaggtaa gagcggcggg cgcaagggcg gcgggcggga ttttgccggc cgcgggcgag
     2461 ccgggctgct cgtccgcttt cggcgaacgc ggaacccgac ccacccgcgg cttagggtca
     2521 cttgatcttc ttggccggca tgtcgcgacc tccctggtgg aggatcaggc cggtggcgcg
     2581 gccggtcgcc gggtcgagct ggaatgtgat ctgcgcgtcg acaacacgca ggaagtattc
     2641 ggtttcgccg gcggggaaga tttcgaattc ctgctggccg gtggcctgcg agaagaagcg
     2701 gccgtcgcgg gcgcgaaacg tgaggacaaa ccccggcgcg agttcgtagt cgccgaccag
     2761 acgcgcgaag accgccgggt cgatcgggac caccgcccgc tccgccatga ggctgtccgc
     2821 gagcggtccg gtgacccggt cgcagcgggt gtcgacgccc gactggtgga tcaggtgggc
     2881 caccaccgcg cccgcctggt cgcggatgaa aacgagtgtc gtcgaattgt cgttctcgaa
     2941 gaagaatttg tcgggggcct cggggaagac ctggaagacc gggccgccgg tccgctgcga
     3001 gtagaggttg tcgccgtcgc ggcgaatcac ccggtacgag ttgtcgccga cccagtagac
     3061 gccgacgagg gcatcgaacg aggcggggtc ggccgccgcc ggtgttttcc ggaccggccg
     3121 gtcatagggc tggtcgaaca tgatcgcggc gagcgtggag ccgaggccgg cggcattggc
     3181 cgacatgttg ttgcacagga cgatgatggt cagccggtcg tcggggtagc gggcgatgac
     3241 gctggtgaaa ccatcgatgc cgccgccgtg ggaaatctcg cgccggccga accgttccgc
     3301 caccatccag ccgtacccgt agttctccag ggcgggggtg tacatctgct tccgggagtc
     3361 ttccgagaga acccggtcgc cgtagagcgc ctggtcccag atgagcaggt cgccgaccgt
     3421 ggaggcgagg gcgcccgcgg catacgggag ggtcatcgag atcggatcgg ccgggacgag
     3481 tgtatcttcc ggagccacgt agccgaccgc acggttgggg gtccgctcgt tcggggggta
     3541 ggtcgtgtgc gtcatgccga gggggcgaaa gatgtgctcc gccaggtaat cggcgtaggt
     3601 ctgccccgag acagcttcga taatcagccc gaggaggaag tagccggagt tgcagtagcg
     3661 gtacatcgcc cccggctcga actgcagcgg cagatctttg aaggaagcga caatttcgcg
     3721 ggccgtcatg tccaagccgc ggcgagccat gaggctgtcg atgtcggtgt agctgactag
     3781 gccggaggtg tgggtgagaa ggtggtggac cgtgattcgg tcgccggtgg ctttcgggaa
     3841 gtcggggagg aatttcgaga tcgggtcgtg gacgctgagt gtcccctgct cctggagctg
     3901 caggatggcg gccgcggtga actgcttggt gaccgagccg atgaggaact cggtctcggg
     3961 ggtgttgggc tggccgtggg cgacgtcggc catgccgtag cctttgacga aaacgggaac
     4021 gccgtcgtcg gccacaagca cggctccctg gaaccggagg acatcgcagg cggcgctgag
     4081 ataggcatcg agttcggccg ttttgtcggc ggccggcacg gtggccgcca gcaggagcgc
     4141 agcggcgaac accagcacgc cggcggttgt acggagcagg aacgatttgc gcataccgta
     4201 cctttcacag caggatagag cgggttcgat ttgtcctatc agacgaatcc acgggcgtaa
     4261 tgttgccgtc aaatgtactg cgcccgtggg gggcgaagcg agcgcctgct actccatctt
     4321 ctccacgcag ccggcgatca ggtcgcgcag gatcggctcg gtcgcactcg cggccccgat
     4381 cacatcggcc agcgagcagg gatgcatgtt gtccggcaga cccatgtcgg tgatgatcga
     4441 gaacgcgagg atcttcgtgc gctggtggtg ggcggtgagc acctccggca ccgtcgacat
     4501 gccgaccgca tcggcgccga acccgcgcag catgcggtac tcagcggcgg tctccaggca
     4561 cggaccggtg agaccgacat acacgccttc ctggaggcgg atcttcagct caagagcaac
     4621 ctgccgggcg agagcctggt accgccggtg gtatacctcg tacatgtcgg ggaaacggtc
     4681 gccgagggtg tcgtcgttgg ggccgatgag cgggttaccg gggaagaagt tgatgtggtc
     4741 tttgatgagc atgatatccc ccggcgcgaa attcgggttg agaccgccgg cggcgttgga
     4801 gacaatgagg gtgtggatac cgagcgcttt gagggtgcgc acgggaaaag cgatctgtgg
     4861 aaacgagtac ccctcgtagt agtggaaccg tccctgcatg cagaccaccg gtttgccgcg
     4921 caggcggccg aagaggaggc ggccggcgtg cgactcgacg gtggagacgg ggaaatgcgg
     4981 gatcttctcg tagctgaccg tgccgaccat gtcgatgccg tcgaccagct tccccaaacc
     5041 ggtgccgagg atgatgccga tccggggggt gaaggcgatc tgcgaggtga tatagccgac
     5101 cgcgtcgttc agcatggcgc ggaattcctc tattgtctgc atgggtacg
//
LOCUS       DAOOKS010000035         5035 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_20029_length_5035_cov_3.250402, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000035 DAOOKS010000000
VERSION     DAOOKS010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5035
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20029_length_5035_cov_3.250402"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..1873
                     /gene="secD"
                     /locus_tag="PK112_01110"
     CDS             149..1873
                     /gene="secD"
                     /locus_tag="PK112_01110"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HPC10680.1"
                     /translation="MSKNNWRIGLTLVLVVAALFAFWHTFSLWTMSDAEKARMQEEQP
                     GELLKLQQKAIRLGLDLQGGIHVVLRVDMAELAPAERDGAVDRAIAIIRNRVDGLGVA
                     EPTIQKQGTDRIIVDLPGYTDANRAEELIGQTALLEFKLLETMDNAQLLLTKIDSAVF
                     GYEKAKAGAAPETPGEPAPTAPTADSAAQEPDVMAELMGDSATKDTTEDPFAFDENTA
                     DAENEKPLSSRLSPALYSSQSGEGWPGFYVAKDQKERIDRWIGLPEVQALMPIDVQWA
                     WSTRSEIRESREVYTLYLLKRKVQFLGRFLENIRLGQGQYGEYTVDFSLSGDGSARFA
                     QLTGANIGKPLAIVLDNRVESAPIINSKIRTNGQITMGGGASIKDAQNLEIVLKAGAL
                     PAPVNIIEKNVVGATLGADSIRKGFYSALLGLCLVLLYMAVYYRISGLIADIGLLFNI
                     FFLLAVMAGLSATLTMPGIAGIILTMGIAVDSNILIFERIREELRTGKTVRAAIEAGY
                     DRAFVAIFDSHVTTLITAGALFLLGSGSIKGFAVTLFWGVAISLYTAYVVTKQIFDIR
                     KSYRSLSI"
     gene            1893..2819
                     /gene="secF"
                     /locus_tag="PK112_01115"
     CDS             1893..2819
                     /gene="secF"
                     /locus_tag="PK112_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HPC10681.1"
                     /translation="MFTIVPETNINFIGARKIAFAISIVLVIGGLFATVLVWTGKADL
                     GIDFAGGTMIYAAFEKPVAIEDLRMAVSSAAPDAQITQLQNFEKPNAFILKTKRPTVE
                     GEGQQRLTAIEGAIRQRFTDNSFEKVSEHIIGPAVGESLRSDTQKAVLLSLLGILVYI
                     WIRFDFRFGVAAAVATFHDVLAVLGIMLLLGFEFDLLLVTALLTLAGYSLTDTVVVFD
                     RIRENLKKWRTKGEFAGAVNRSINEIISRTINTGGTTLVSILALTIFGGEVLRTFSVA
                     MVLGIIVGTYSSWFVASPIVVEWEARRPKRFK"
     gene            complement(2894..3742)
                     /locus_tag="PK112_01120"
     CDS             complement(2894..3742)
                     /locus_tag="PK112_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10682.1"
                     /translation="MLRLLPIVLAALLIAGCGGEPKTEAELREAGKKAFVKEDFAKAR
                     DYLGRLVRLHPSDRDALYFLGMAYSKDLKYDSALYYLERVNTLYEDDREANLELYRIG
                     MLTQDAKVAKDAIGALIRTGDPADMYYEDLARLSLMLKQYALATRYFRILLEREPDQP
                     QRYLDAANAVAGLDSLAVAVALLDSAAARFGARPEFLLNKSLYLTAQEKYGESEAIIR
                     GLIAADTGNLGYKVNLAHVLSSTDSEAKRREAYALYHDLKGRVDARFAVDSLMADLAR
                     KLDSAQ"
     gene            3826..>5035
                     /locus_tag="PK112_01125"
     CDS             3826..>5035
                     /locus_tag="PK112_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10683.1"
                     /translation="MVPRPALFLSVLLASSPVQAVDSSHPKVQAPVAVVAPMDTESTA
                     DQPAALADRLGRSLANHLAHLAAMTGRGVTVTLRETPAEDRGLLDSLAGEGVREVLVV
                     RWRGRAGERTGEIRDAHRRRYKAVVEDCLQIEAAYERRQLTAHGWEVAAGGTAAAAGR
                     PDPNARRRPGRTLAGEPYERVVQRLVAQIGDELPPAEPLARALRATAPVKVYLGKELA
                     ADSGAAMALIRRIVDLASTHLAAQFGVALRIVSAAPLPENAADEGSLAAGYPEFRRAV
                     SVSADTLAVAFHRMLSREQYFGGGVREEIGMAQLGRRRVYMDLLPAAAPGDTLWQAMA
                     NALTLLHEIGHALGGIHVSDVNSVMAHGANWVNAAAFDRVNEKIIGAALTGRLRFDDP
                     AEYLATVARVL"
BASE COUNT          856 a         1593 c         1730 g          856 t
ORIGIN      
        1 gcaccctctt ctgggccgcc agttcacgct ggtcggcgcc aaaccctccg gcacgggctg
       61 accgcccgtc cccgcgccgc tctgccggcg ccgaccgcag ttccgacatt tctcgaacgt
      121 gctgataaaa gactgaaagg gaaacagtat gtccaaaaac aactggcgta tcggcctgac
      181 cctggtgctc gtggtcgcgg ccctcttcgc cttctggcac acgttcagcc tctggaccat
      241 gagcgacgcc gagaaggcgc ggatgcagga ggaacaaccg ggcgagctgc tgaaattgca
      301 gcagaaagcg atccggctgg gcctcgacct ccagggagga atacacgtcg tgctccgcgt
      361 cgacatggcg gagctggccc cggccgagcg cgacggtgcg gtggatcggg cgatcgcgat
      421 tatccgcaac cgtgtcgacg gcctgggggt ggccgagccg accattcaga aacaggggac
      481 cgatcgcatc atcgtcgacc tccccggtta caccgacgcc aaccgggcgg aggagctcat
      541 cgggcagacg gcgctgctgg agttcaagct cctggagaca atggacaacg cgcagctcct
      601 cctgaccaag atcgactcgg ccgttttcgg ctatgagaag gcgaaggccg gggcggcgcc
      661 ggaaactccg ggcgagcctg cgccgaccgc tcccacggcc gattcggcgg cccaggagcc
      721 ggacgtcatg gccgaactga tgggggattc cgcaacgaag gacacgaccg aggatccgtt
      781 cgcgttcgat gagaacacgg ccgacgcgga gaacgagaaa ccgctgtcct cgcggctgtc
      841 gccggcgctg tactcctcgc agagcggcga gggctggccg gggttctacg tggccaagga
      901 tcagaaggag agaatcgacc ggtggatcgg cctcccggag gttcaggcgc tcatgccgat
      961 cgacgtgcag tgggcgtggt cgacccggtc ggagattcgg gagtcgcgcg aggtctacac
     1021 gctgtacctg ctgaagcgga aggtgcagtt tttgggcagg tttctggaga acatccgcct
     1081 cggccagggc cagtacggcg agtacaccgt tgatttctcg ctgtcgggtg acggctcggc
     1141 ccggttcgcg cagttgaccg gcgcgaatat cggcaagccg ctcgcgatcg tgctggacaa
     1201 ccgcgtggag tccgccccga tcatcaacag caaaatccgc accaacgggc agatcacgat
     1261 gggcggggga gcgtcgataa aggatgccca gaacctcgag atcgtgctca aggcgggcgc
     1321 gctgccggcc ccggtgaaca tcatcgagaa aaacgtcgtg ggggcgaccc tcggcgccga
     1381 ctcgatccgc aaagggttct actcggccct gctggggctg tgcctggtgc tgttgtacat
     1441 ggccgtctac taccggatct cggggctgat cgccgacatc ggcctgctct tcaacatctt
     1501 cttcctgctg gccgtgatgg ccgggctgag cgcgaccctc acgatgcccg ggatcgcggg
     1561 tatcatcctc acgatgggta tcgcggtgga ctcgaacatt ctgatcttcg agcgcatccg
     1621 cgaggagctg cgcacgggga agacggtgcg ggcggccatc gaggcgggct atgaccgcgc
     1681 cttcgtcgcc attttcgact cgcacgtgac gacgctcatc acggccggag cgctgttcct
     1741 gctcgggtcg ggctcgatca agggatttgc ggtcacgctg ttctggggtg tggcgatctc
     1801 gctctacacc gcctacgtgg tgaccaagca gatcttcgac attcgcaaat cgtaccggtc
     1861 gctgagcatc tagaacggag agagaagacg acatgttcac aatagtaccc gagaccaaca
     1921 tcaacttcat cggcgcgcgg aagatcgcct tcgcgatctc catcgtgctg gtgatcggcg
     1981 gcctgttcgc cacggtcctg gtctggacgg gcaaggccga cctgggaatc gatttcgccg
     2041 gcgggacgat gatctacgcc gcgttcgaaa agccggtcgc gatcgaggac ctgcggatgg
     2101 cagtgagctc ggccgcgcct gatgcccaga tcacccagtt gcagaatttc gaaaagccga
     2161 acgctttcat cctcaagacc aagcgaccga ccgtggaagg agagggacag cagcggctca
     2221 cggccatcga gggggcgatc cggcagcgtt tcaccgacaa ctcgttcgag aaagtgtccg
     2281 aacacatcat cggcccggcc gtcggagaat cgctccgcag cgatacccag aaagcggtgc
     2341 tcctgtcgct tttgggcatc ctcgtctaca tctggatccg cttcgacttc cggttcggcg
     2401 tggccgcagc cgtcgctacc ttccacgacg tcctggcggt tctggggatc atgctcctgc
     2461 tcggtttcga attcgacctc ctgctggtga cggcgctgct gacgctggcg gggtattcgc
     2521 tgaccgacac ggtggtcgtg ttcgaccgga tccgggagaa tctcaagaag tggcggacca
     2581 aaggggagtt cgccggcgcc gtcaaccggt cgatcaacga gatcatctcg cggacgatca
     2641 acaccggcgg cacaacgctc gtgtcaattc tcgccctcac catcttcggc ggcgaggtgc
     2701 tccggacgtt ctcggtggcg atggtgctgg ggattatcgt cgggacatac agctcctggt
     2761 tcgtggccag cccgatcgtg gtcgaatggg aggcccgccg acccaagcgc ttcaaatgag
     2821 aagctgatct tctggcagcg gccggcggtt catccgccgg cccttttttt ggccgggcgc
     2881 ggcccgcgca cggtcactgc gcgctgtcga gcttccgggc gagatccgcc atcagggaat
     2941 cgaccgcgaa tcgggcgtcg acccggccct tcaggtcgtg gtagagcgcg taggcctcgc
     3001 gccgcttcgc ctcgctgtca gtcgacgaga ggacgtgggc gaggttgacc ttgtacccga
     3061 gattgccggt gtcggcggcg atgagcccgc ggatgatcgc ctcgctctcc ccgtacttct
     3121 cctgggcggt caggtagagc gatttgttga gcaggaactc cggccgcgcg ccgaaacggg
     3181 ccgccgcgga gtcgaggaga gcaaccgcca cggcgaggga gtcgagcccg gcgacggcgt
     3241 tggcggcgtc gagatagcgc tgcggctgat cgggctcgcg ctcgagcagg atgcgaaaat
     3301 agcgggtcgc gagcgcatac tgcttgagca tcaggctcaa ccgggccagg tcctcgtaat
     3361 acatgtcggc cgggtcgccc gtgcggatga gggcgccgat ggcatccttg gcaaccttcg
     3421 catcctgcgt gagcatgccg atccggtaga gctcgagatt cgcctcccgg tcgtcctcgt
     3481 agagagtgtt gacgcgctcg aggtagtaga gggccgaatc atacttgagg tctttcgagt
     3541 aggccatccc gaggaaatag agcgcgtcgc ggtcggaggg atggaggcgg acgagccggc
     3601 cgaggtagtc gcgggctttg gcgaaatcct ccttgacgaa ggctttcttc ccggcctcgc
     3661 gaagctcggc ctccgtcttg ggttcgccgc cgcagccggc gatcaggagg gcggccagga
     3721 cgatcgggag cagtcgcagc atagatcctc tcaggctgtc ggttcgcgca tatgatagga
     3781 acacgcggct tgaatttcaa gccctccggc cgtaatatat gccctatggt ccctcgtccc
     3841 gcccttttcc tgtcggtgct gctcgcgtct tcccccgtgc aggcggtcga cagcagccat
     3901 ccgaaagtcc aggcgccggt ggcggtggtg gcgccgatgg atacggagag caccgccgat
     3961 cagccggcgg cgctcgccga ccggctcggg cggtcgctgg ccaaccacct cgcgcacctg
     4021 gccgccatga ccgggcgcgg ggtgacggtg accctgcggg agacgccggc ggaggaccgc
     4081 ggtctcctgg actcgctggc cggggaaggg gtgagagagg tgctggtggt ccgctggcgc
     4141 gggcgggcgg gggagcggac gggtgaaatc cgcgacgccc accggcgacg gtacaaagcg
     4201 gtggtcgagg actgcctcca aatcgaggcc gcgtacgaac ggcggcagct caccgcgcac
     4261 ggctgggagg tggcagccgg gggaactgcg gccgcggcgg ggcggccgga cccgaacgcc
     4321 cgccgccgcc ccggccgcac gcttgccggc gagccctacg aacgtgtggt gcagcggctg
     4381 gtcgcgcaga tcggggatga gcttcccccg gccgaacccc tggcccgcgc cctccgcgcg
     4441 accgcgccgg tgaaagtcta cctcgggaaa gagctggcgg ccgattcggg cgcggcaatg
     4501 gcgctcatcc gccggatcgt cgacctcgcc tcgacacacc tcgccgccca gttcggcgtc
     4561 gccttgcgca tcgtctcggc ggcgcctctg ccggagaacg ccgccgatga aggatcgctg
     4621 gcggccgggt accccgaatt tcgccgggcc gtgtccgtct ccgccgacac gctggccgtc
     4681 gctttccacc ggatgctctc gcgcgagcaa tacttcggcg gcggagtgcg ggaggagatc
     4741 ggcatggccc agcttggccg gcggcgcgtg tacatggact tgctgccggc ggcggcgccg
     4801 ggagacacgc tgtggcaggc gatggcgaat gcgctgacgc tgctgcacga gatcggccac
     4861 gccctgggag ggattcacgt gtccgatgtc aactcggtca tggcgcacgg cgcgaactgg
     4921 gtgaacgcgg cggcctttga ccgggtcaat gagaagatca tcggcgcggc cctgaccggc
     4981 cggttacggt ttgacgaccc cgccgaatac ctcgctacgg tggcgcgcgt gctcg
//
LOCUS       DAOOKS010000036         4956 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_20468_length_4956_cov_3.235666, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000036 DAOOKS010000000
VERSION     DAOOKS010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4956
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20468_length_4956_cov_3.235666"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="PK112_01130"
     CDS             complement(<1..295)
                     /locus_tag="PK112_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10684.1"
                     /translation="MASTIGRKDVIGWSLYDLANTIYSMNITSLYLKRYIVEDLGRDD
                     RWFDIPFALSMFLAALLLPAFGAMSDHSVKKKLFVFLFTLTCCLAVGGMAPLPP"
     gene            complement(304..873)
                     /locus_tag="PK112_01135"
     CDS             complement(304..873)
                     /locus_tag="PK112_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HPC10685.1"
                     /translation="MTDRSDIGLTREEAFRLVLEKIGHTNLLKHILAVEAEMRRLAER
                     LGEDVGLWGLTGLLHDLDYNETVGDAGAHTFVTESWLQPYHLPAAMLYAIRAHPGHVP
                     CRSRLDWALFATDPATGFIVACVLMHPAKRLDVIDADFMLRRFKEKRFAAGASRDNMA
                     ACTSLGLELEEFLLLVHAGMMTIRSDLQL"
     gene            complement(1002..2879)
                     /locus_tag="PK112_01140"
     CDS             complement(1002..2879)
                     /locus_tag="PK112_01140"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPC10686.1"
                     /translation="MRRSLLFSLAALAILLGGLALTGRAADKIKIEKLDDLPRHTYRI
                     AVKAAEVPVNPEALAALAAAVKADLLADLERYEIPDATTIKNYHSDLGAIALLEADFA
                     AYETHIAAVRDLEDKEAQKLTTGLTGRAYAAAMAAPEAQRPEAYRTAYAALVNALPYD
                     VVEAQLRSTKGMAEMMSENLVLGLAESKYQPLVDSTGGVISKDVALSLLKTATMLRTI
                     IPYRESIAGVIDAYLAAHAVDKPDIWAERQVALSGSEGGQPVLVTIWDSGLDTEIPAI
                     AQVLWTNADETPGNGSDDDGNGFVDDVHGIAYTLHSDKTPDLLYPIGEVGAERPRLQR
                     QMKGLTDISMAVDSKEAAELKQELSGLQQAEAQTFIQNIALYGNYCHGTHVAGIAMAG
                     NPYIRLMPSRLTFGYTLVPEKPTLEQARKDSAAADEAIAYYRKHGVRVVNMSWGGSLA
                     SVEQALEAHNAGGTPEERRELARQIFEIGRSGLYAAMQNTPEILYVTAAGNEDNDVKF
                     EEVIPSAFDLPNIIVVGAVDQAGDETGFTSFGNVDCYANGFEVESFVPGGDRMKLSGT
                     SQASPQVVNLAAKVLALKPELTPAQVRELILSGCDEKQVGDRAVKLINPKKTMGLATP
                     R"
     gene            complement(3008..3271)
                     /gene="ffs"
                     /locus_tag="PK112_01145"
     ncRNA           complement(3008..3271)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PK112_01145"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(3360..3432)
                     /locus_tag="PK112_01150"
     tRNA            complement(3360..3432)
                     /locus_tag="PK112_01150"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3397..3399),aa:Ala,seq:cgc)
     gene            complement(3519..4133)
                     /locus_tag="PK112_01155"
     CDS             complement(3519..4133)
                     /locus_tag="PK112_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10687.1"
                     /translation="MRQQLRRALVLGLLLLPASASAAPTWADGVPKVIAHGGVGLKLG
                     LINTGALTLTGRPTPWDSVAVAGKELDPKAAPSGQVFFDVPLTRAFLLLFSVDIYDLR
                     FHPLSERFIDVAAGFKLAFYKHHQRLAWRPGAAVGLGYLADIGFMRPTTYLTLKAFSE
                     LVLLPRGKWAVVLELGFMAVPSGGNSAVDVKIAPSLFLRAGLLY"
     gene            4274..>4956
                     /gene="dnaX"
                     /locus_tag="PK112_01160"
     CDS             4274..>4956
                     /gene="dnaX"
                     /locus_tag="PK112_01160"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HPC10688.1"
                     /translation="MTYQVLARKYRPPTFEGVVAQAHVTRTLQNAVRNGRVGSGYLFC
                     GPRGTGKTTTARILAKALNCVNGPTPEPCGVCPACRDITGGNSLDVLEIDAASNTGVD
                     DVRALRERINLTPAGGKKKIYIIDEVHRLSGAAFDALLKTLEEPPEHVVFIFATTEPT
                     KVPETILSRTQRFDFKRVSALDLAQHLQHIAEQEGMAISESAVALLARRGDGSVRDAV
                     RPRYVERLD"
BASE COUNT          851 a         1556 c         1661 g          888 t
ORIGIN      
        1 ccggcggcag gggggccatc ccccccaccg cgaggcagca ggtgagcgtg aagagaaaca
       61 cgaagagctt cttcttgacc gagtggtccg acatcgcccc gaaggccggc agaagcaggg
      121 ccgcgaggaa catcgaaaga gcgaacggga tatcgaacca gcggtcgtcg cggccgaggt
      181 cttcgacgat gtaccgtttg aggtagagcg aagtgatgtt catcgagtag atcgtgttgg
      241 cgagatcgta gagcgaccac ccgatgacgt ccttccgccc gatagttgag gccacgcgca
      301 gtcctatagc tggaggtcgc tccggattgt catcattccg gcgtgcacca ggaggaggaa
      361 ctcctcgagt tcgagtccga ggctggtgca ggccgccatg ttgtcgcgcg aggcgccggc
      421 ggcgaagcgc ttttccttga agcgccgcag catgaagtcg gcgtcgatca cgtcgagccg
      481 cttggctgga tgcatgagca cgcaggcgac gatgaacccg gtcgccgggt cggtggcgaa
      541 gagcgcccag tcgagccggc tgcggcatgg cacgtggccg gggtgggcgc ggatcgcata
      601 gagcatcgcg gccggcaggt gatagggctg cagccagctc tccgtgacga aggtgtgcgc
      661 cccggcgtcg ccgaccgtct cgttataatc gagatcgtgg agcaacccgg tcaggcccca
      721 caacccgaca tcctcgccca gccgctccgc cagccggcgc atctccgcct ccacggcgag
      781 aatgtgtttg aggagattgg tgtgcccgat cttctccagg acgagccgga aggcttcctc
      841 gcgggtgagg ccgatgtcgc tccggtcggt catgacgccc aataaaggcc ctgcgcagaa
      901 gcgtgtcaag gaacgatgcg gcggcccgcc cgctccccgc cgcaggcggc cgccggacca
      961 ccggtccggc ggcgcggttt ttccccgccg gggcagagaa cctacctcgg cgtcgccagt
     1021 cccatcgtct tcttgggatt tatgagcttt accgcccggt ccccgacttg tttttcgtcg
     1081 cagccgctga ggatgagttc gcggacctga gccggggtga gctccggctt cagggcgagg
     1141 actttcgcgg ccaggttgac gacctgcggc gaggcctggg aggtgccgga gagcttcatg
     1201 cggtcgcccc cgggcacgaa actctcgacc tcgaaaccgt tagcgtagca gtcgacgttg
     1261 ccgaaggagg tgaagccggt ttcgtcgccg gcctggtcga cggcgccgac gacgatgatg
     1321 ttggggaggt cgaaagcgct ggggatgacc tcctcgaatt tcacgtcatt gtcctcgttg
     1381 ccggcggccg tgacatagag gatctccggc gtgttctgca tcgcggcata gagcccgctc
     1441 cggccgattt cgaaaatctg ccgggccagt tcccggcgct cctcaggcgt cccgccggcg
     1501 ttgtgcgctt cgagcgcctg ctcgacgctc gcgagcgacc cgccccagct catgttgacc
     1561 acccgcacgc cgtgcttccg gtagtacgca atcgcttcgt cggcggcggc ggagtctttc
     1621 cgggcctgct cgagcgtggg tttctcgggg acgagggtat acccgaaggt gaggcgggag
     1681 ggcatgaggc ggatgtaggg gttgccggcc atggcgattc ccgccacgtg tgtgccgtgg
     1741 cagtagttgc cgtagagggc gatgttctgg atgaacgtct gggcctcggc ctgctgcagc
     1801 ccgctcagtt cctgcttgag ctccgccgct tccttactgt ccacggccat gctgatgtcg
     1861 gtcagtccct tcatctgccg ctgcaggcgc gggcgctccg cccccacttc gccgatcggg
     1921 tacagcagat ccggggtttt gtcggagtgg agcgtgtagg cgatgccgtg cacatcgtcg
     1981 acaaacccgt tcccgtcatc gtcactcccg ttgccggggg tctcgtccgc gttggtccac
     2041 agcacctggg cgatcgcggg gatctcggtg tcgagcccgg agtcccagat ggtcacgagc
     2101 accggctgtc ccccctcgct cccgctgagc gcgacctggc gctcggccca gatgtccggt
     2161 ttgtcgaccg cgtgcgcagc caggtaagcg tcgatcaccc cggcgatgga ttcccggtag
     2221 gggatgatcg tccgaagcat ggtggcggtt ttcagcagcg agagcgcgac gtccttgctg
     2281 atgacgccgc cggtcgagtc gaccagcggc tgatatttcg attccgccag tccgaggacg
     2341 aggttctcgg acatcatttc ggccatcccc ttggtcgacc ggagctgggc ctcgacgaca
     2401 tcgtagggca gagcgttgac gagcgcggcg taggcggtcc gataggcctc tgggcgctga
     2461 gcctccggcg ccgccatagc cgccgcgtaa gcccggccgg tcagaccggt cgtcagcttc
     2521 tgcgcctcct tgtcctccag gtcgcgaacg gcggcgatat gggtctcgta ggctgcgaaa
     2581 tccgcctcca ggagcgcgat cgcgccgagg tcggagtggt agttcttgat ggtcgtggca
     2641 tcggggatct cgtagcgctc gaggtcggcc agcagatccg ctttcacggc cgccgcgagc
     2701 gcggcgagcg cctccggatt aaccgggacc tcagcggcct tgaccgcgat tcggtaggtg
     2761 tggcggggga ggtcatcgag cttctcgatc ttgatcttgt cggccgcgcg gccggtcaag
     2821 gccaggccgc caagcagaat cgcgagagcg gcgagggaga agagcagtga acgtcgcatg
     2881 cgggctcctt atgtcagacg tgtcgcacca tgttattgct gcgtttgtac gtttcggggc
     2941 ggggtttggt tgcgggcggc cgggaaacaa gacggcccgc cggggaaacc gacgggccgc
     3001 gattttactg tgcacccacc gttgagccgc ccccggacaa ccctcgaggc agttggctcc
     3061 aaccgggcgc tcccgcaaca cacggaacgg actacttacg gctgctacct tccggtcctg
     3121 accgggttca taggtttccg ttgtacggga cccagccttc aacaccactt acccgttggc
     3181 gaagtccggc gaccgcaccc gcgggtggga attcggcccc ggtatagcgg gttccgggta
     3241 cagggcaccg ctaactcccc gcctagcaca ggctcgtcgc acgagcgaac agccggtgac
     3301 aacaatggcc tccttgcggg aggccggtcc actgctgtcg cgcacacgct atttttaagt
     3361 ggagcagatc gggatcgaac cgacgacctc aacgttgcga acgttgcgct ctcccaactg
     3421 agctactgcc ccagggatct tcacatcacg gcccatacta taatcgtccc ggaaggattt
     3481 gccaagcgat tttttccggc cccgtctctc cccctctcct agtagaggag tcctgcccgg
     3541 agaaagaggg agggtgcgat tttcacgtcc acggcactgt tgcccccgct cggcacggcc
     3601 atgaaaccga gctcgagcac gaccgcccac ttcccccgcg ggaggagcac cagttcgctg
     3661 aacgccttga gagtgaggta cgtagtgggg cgcatgaagc cgatgtcggc caggtagccg
     3721 agcccgaccg cggcccccgg ccgccaagcc agccgctgat ggtgcttgta aaaggcgagc
     3781 ttgaacccgg ccgccacatc gataaaccgc tcgctgaggg ggtggaaccg caagtcgtag
     3841 atgtcgacgg agaataggag aagaaaagcg cgggtcaggg ggacatcgaa aaacacctgt
     3901 ccgctgggag cagccttggg atcaagctcc ttgcccgcca cggccaccga atcccagggc
     3961 gtgggtcgcc cggtgagagt cagggcgccg gtgttgatca gccccagctt caggcccacc
     4021 ccgccgtggg cgatgacttt cggcactccg tcggcccacg tcggcgccgc cgaagcggag
     4081 gccgggagca agagcaggcc gagaacgagg gcgcgtcgaa gttgttgccg cacaaggggg
     4141 gcctttcgat agaggatttt ctcgccacat agacaggaat tacctgctgt ttgtcaatac
     4201 gaatagcggc gcccttcgga cgcggaaaaa ttgtgattgg gaatgaggca gatattgcat
     4261 atactggaga gttatgacgt atcaggttct ggcccggaag tatcgcccgc cgacgtttga
     4321 aggcgttgtg gcacaggcac atgtgacccg cacgctgcag aacgctgtgc gcaacgggcg
     4381 ggtgggatcg ggttatctgt tctgcggccc gcgcggcacc gggaagacca ccactgcccg
     4441 gatcctggcc aaagcgctca attgtgtcaa cggcccgacc cccgaaccgt gcggggtctg
     4501 ccccgcctgc cgcgacatta ccggcggcaa ctcgctggat gtgctggaga tcgacgccgc
     4561 ctccaacacg ggggtcgacg atgtccgggc tctgcgcgag cggattaacc tgacccccgc
     4621 cggcggcaaa aagaaaatct acatcatcga tgaagtccac cggctctcgg gggcggcctt
     4681 cgacgcgctt ctgaagacgc tcgaggaacc gccggagcac gtggtgttca ttttcgccac
     4741 caccgagccg acgaaagttc cggagacgat cctctcccgg acgcagcggt ttgatttcaa
     4801 gcgggtgtcc gcgcttgacc tggcccagca tctccagcat atcgccgagc aggagggcat
     4861 ggcgatcagc gagagcgcgg tggcgctcct ggcgcggcgg ggggacggtt cggtgcgcga
     4921 cgcggttcgg ccgcgttatg tcgagcggct cgatta
//
LOCUS       DAOOKS010000037         4890 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_20817_length_4890_cov_2.937746, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000037 DAOOKS010000000
VERSION     DAOOKS010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4890
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20817_length_4890_cov_2.937746"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="PK112_01165"
     CDS             complement(<1..687)
                     /locus_tag="PK112_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10689.1"
                     /translation="MDVPTYKRCAARGPEAAPPELPAAAALYTGGGIPQLFRRLGTAE
                     GADAWLYVPAGTLDSLVPTTPATEGQFASAEGLTVDDSGRFCPLVVGGAVRGFLSGMD
                     RPDGAALKAAVLDWLRTREIATQATLLPAQAELAAALVACDLSPERLLRNALNLLVEQ
                     VPSGLAAVYGESEGVYGLRTAVGDIAMWDMLEGTLPHATVGRWMEGLAVGRHFLPAGF
                     LPERPMLLDAA"
     gene            complement(724..2307)
                     /locus_tag="PK112_01170"
     CDS             complement(724..2307)
                     /locus_tag="PK112_01170"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HPC10690.1"
                     /translation="MALPWAPFGAALFMLALLVLLRLYGKYLADQYREQFRNLVVGVG
                     LFGLVAVLYLGEATGATAAVPFISQPPFLEVSAAILAITGAVLTASGASVWLPARRTG
                     KILHDVPSEPLRREAEIVRAQLQDALALGAPLADSFTAIARVLKAALGGELSSLAMAY
                     GEWDIRRYTAGGNGTHLTEKSLAREAAGLPQENDSPPPDRPFVTVDFEHFLEPALAAV
                     LRRQQLAAALLVPLGDPGGAAMLVAAARSAEEFGERERYLAAQVRFPLRLAVAVERER
                     DRLARGMKRSEALARLSAQAAARLGDIDRLFAEAADVLRRSTGAAAVRIALFEDDGQF
                     LRSRAAAAAHPVENAVPATGYLIRSLMPLHQSVQATGEALLISQAEAAERMSAAEAQQ
                     IYQSDLSTALLSPITAGTETLGIIALADRRGSRRFGFTDDDRTFAAAVAAVLAQAVRL
                     HETPWGALTWRDRESGEDADRRAELRGRIRSSLTGILGSVEMLRARPVGPEAERFVEI
                     IDRSARRIETIFSEETPAR"
     gene            complement(2467..3087)
                     /locus_tag="PK112_01175"
     CDS             complement(2467..3087)
                     /locus_tag="PK112_01175"
                     /inference="COORDINATES: protein motif:HMM:NF013514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HPC10691.1"
                     /translation="MRQGDFFGPLVVAALAAPDSALADLKGLGVRDGKGIADRRLLTI
                     DEQLRRAYPFAVEIMPPEEYNRAYEKIRNLNRLLADLHARAIDRLLRDHPADLVVVDQ
                     FGKTALVAEAVAFHGHSVALEQRFRAEEIPQVAAASIIARAEFIRQLQTLSREMGVEL
                     PKGAAAIVDRAGAALVRQHGRDILRKTAKIHFKNYQRVINPSLFPS"
     gene            complement(3125..3685)
                     /gene="efp"
                     /locus_tag="PK112_01180"
     CDS             complement(3125..3685)
                     /gene="efp"
                     /locus_tag="PK112_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HPC10692.1"
                     /translation="MYSPSDFRKGLRIIVDEQPFYIVDYQHVKMGRGRANIRTRLKHM
                     KTGAVIERVFGSNDTFKAPDTQERKMQYLYENAGEFSFMDTESFEQVVILADSLGDAK
                     WYLVENSEYRVFFLDGQPISVDLPASVTLEVIYTEPAARGDTVSNVTKPAKLSTGLEV
                     KVPPFVKEGDMVKVDTRTGEYLERAN"
     gene            complement(3882..>4890)
                     /locus_tag="PK112_01185"
     CDS             complement(3882..>4890)
                     /locus_tag="PK112_01185"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPC10693.1"
                     /translation="RIQTINAAAERILGLREGEARGRFHAELMPRELAEAIAGPLVDE
                     AGGEYREVTPAGLPDTPVLGIAASTVWDGEGEPIGAALLINDATEITQLREQLERRLR
                     LAALGEMSGGLAHQLRNSLGAIAGYLNLLKRRLGKRGVEEPAVAELADEAAQAESLVR
                     RFLEFARPLQADLQAVPLRDLIGQAAASFATREAYPRVEIAVGEIPEVVIPADPLLLR
                     QALLNLIENAANAYSGAAGKIEIDAAAGNGETAIRVRDRACGIAPENVAKIFTPFYSS
                     RPSGTGLGLALVSKIVDLHKGRIEVDSRVGRGSTFTITLPVGAPQPAAAEGPAEMRVR
                     G"
BASE COUNT          790 a         1815 c         1514 g          771 t
ORIGIN      
        1 ggcggcatcg agaagcatgg ggcgttcggg gagaaacccc gccggcagaa agtggcgccc
       61 cacggccagc ccctccatcc agcgccccac ggtcgcgtgc ggcagcgtcc cctcgagcat
      121 gtcccacatg gcgatgtcgc cgaccgccgt ccgcagcccg tacacgccct ccgattcgcc
      181 gtacacggcc gccagtcccg atgggacctg ctcgacgagg aggttcaggg cgttgcgcag
      241 cagccgctcc ggcgacaggt cgcaggccac cagcgccgcc gccagttccg cctgcgccgg
      301 aagcagcgtc gcctgagtcg cgatctcccg cgtccgcagc cagtcgagca ccgcggcctt
      361 gagcgcagcg ccgtccggcc ggtccatgcc gctgaggaaa ccgcgcaccg ccccgccgac
      421 cactagagga cagaagcgac cgctatcgtc caccgtcagc ccttccgccg aggcgaactg
      481 accctccgtc gccggcgtcg tcggcacgag gctgtccaga gtgccggccg gcacatagag
      541 ccatgcatcc gcgccttcgg cggtgccgag gcgccggaac aactggggaa tgcctccgcc
      601 ggtgtagagc gcggctgcgg cgggcagctc cggcggggcc gcctccgggc cgcgtgcggc
      661 gcaccgcttg taggtcggca catccatgct gcgtgaccgg gggatttcgc cgatcggcgg
      721 cgatcagcgc gccggcgtct cctccgagaa aattgtttcg atgcgccggg ccgaccgatc
      781 gatgatctcc acgaagcgtt cggcctcagg gccgaccggg cgggcgcgga gcatctccac
      841 cgacccgagg atgccggtca gcgacgagcg aatccggccg cgcagctccg cccggcggtc
      901 cgcatcctcg ccactctcgc gatcccgcca ggtgagcgcg ccccagggtg tctcgtgcag
      961 gcgcacggcc tgggccagca ccgccgccac ggccgcggcg aatgtccggt cgtcgtcggt
     1021 gaatccgaac cggcggctcc cccggcggtc ggccagggca atgatcccca gcgtctcggt
     1081 tcccgcggtg atgggcgaca ggagcgcggt cgagagatcg ctctggtata tttgttgcgc
     1141 ctccgccgcc gacatccgct cggccgcctc ggcctggctg atcaggagcg cctcgcccgt
     1201 cgcctggacc gactggtgca ggggcatgag cgagcggatc aggtacccgg tcgccggcac
     1261 ggcgttctcc accgggtgcg ccgccgccgc ggcccgcgac cgcaggaact gcccgtcgtc
     1321 ttcaaacagc gcgatccgca ccgcagccgc tccggtcgac cggcgaagga cgtcggctgc
     1381 ttcggcgaac agccggtcga tgtcacccag ccgggccgcc gcctgggcgg acaaacgggc
     1441 gagcgcctcc gaccgcttca tcccgcgcgc cagccggtcg cgttcccgtt cgaccgccac
     1501 cgccagccgc agcggaaagc gcacctgcgc ggcgaggtag cgttcgcgct cgccgaattc
     1561 ctcggccgag cgcgccgccg cgaccagcat cgccgcgccg cccggatcgc cgagcggcac
     1621 caacagcgcc gccgccagtt gctggcggcg aagcaccgcg gccagcgccg gctcgaggaa
     1681 atgctcgaaa tccacggtca cgaacggtcg gtccggaggc gggctgtcat tctcctgcgg
     1741 gaggcccgcc gcctcccgcg ccagcgactt ctccgtcaaa tgcgtcccgt tgccgccggc
     1801 cgtatagcgg cggatatccc actccccgta tgccatcgcc agcgaactca attctccccc
     1861 gagcgccgct ttcaacacac gcgcaatcgc cgtgaacgaa tccgccagcg gcgccccgag
     1921 ggcgagtgcg tcctggagct gggcgcggac gatttccgcc tcgcgccgaa gcggttctga
     1981 cgggacgtca tggaggatct tccccgtccg acgcgccggc agccacacgg aggccccgga
     2041 ggccgtcagc acggccccgg tgatcgcgag aatcgccgcg cttacctcca ggaacggcgg
     2101 ctgcgaaatg aacggcacag cggccgtcgc ccccgtcgcc tcgccgaggt agagcacggc
     2161 caccaaaccg aacaggccga cgccgacgac gagattgcgg aactgctcgc ggtattgatc
     2221 cgccaggtat ttgccatata gtctgagcag caccagcagc gccaacatga acaaggcggc
     2281 gccaaacggc gcccacggca aagccataca ccctccggtc gggctaactg attatctttt
     2341 ttcagattat cggcagaacc gggggacggt ttatgcatcg tcggaacttg tcaccggcac
     2401 cggaggcctc gccgtctcag ttccgtatcc ccctgaaaca caaagccttg tggccacgaa
     2461 aaggcctcac gacgggaaca aagaaggatt gatgactcgc tgatagttct tgaaatggat
     2521 cttggccgtc ttccgcagta tgtcccgccc gtgctgccgc accagcgcgg ctcccgcccg
     2581 atccacgatt gccgccgccc ctttcggcaa ttccactccc atttcgcgcg agagcgtctg
     2641 cagttgccgg atgaattcgg cccgggcgat gatggaagcg gcggcgacct gtggaatttc
     2701 ctcggcgcga aaccgctgct cgagcgcgac cgagtgccca tggaacgcca cggcctcggc
     2761 cacgagggca gtcttgccga actggtcgac caccaccaaa tccgccgggt ggtcgcgcag
     2821 cagacggtcg atcgcccggg cgtggagatc ggccagcagc cgattgagat tgcggatttt
     2881 ctcgtaagcc cggttgtatt cctcgggcgg catgatttca actgcgaagg ggtaggcgcg
     2941 ccgcaactgc tcatcgatcg tcaggagacg ccggtcggca attcccttgc cgtcgcgcac
     3001 ccccagcccc ttcaggtccg ccagcgcgct gtcgggcgcc gccagcgcgg cgaccaccag
     3061 cgggccgaag aagtcccctt gccggattcg tcgaccccga tgacgcggcg gtcccccgcc
     3121 gccactagtt cgcccgctcg agatactcgc ccgtccgcgt gtccaccttc accatgtccc
     3181 cctccttgac gaacggcggg actttcacct cgagcccggt ggacagcttg gccggcttgg
     3241 tgacattgct caccgtgtcg ccgcgggcgg ccggttccgt atagatgacc tcgagagtga
     3301 ccgatgccgg cagatcgacc gagatcggct gaccatccag gaagaacacc cggtactcgc
     3361 tgttctccac caggtaccac ttggcgtcgc ccagcgaatc agccaggatc accacctgct
     3421 cgaacgactc ggtgtccatg aaactgaact ccccggcgtt ctcgtacagg tactgcattt
     3481 ttcgttcctg ggtgtcgggc gccttgaacg tgtcgttcga gccgaacacc ctttcgatca
     3541 ccgccccggt tttcatgtgt ttcagccgcg tccggatgtt ggctctcccg cggcccattt
     3601 tcacatgctg gtaatcgaca atgtagaacg gttgttcgtc gactattatg cgcagccctt
     3661 ttctgaaatc cgacggtgaa tacatcaatt cctcctagag aatcggcccc aaatataaca
     3721 aaaagtcgga tccgcaaaag accttttttc cccggccgcc gtggcggccc ggcggggttt
     3781 gccgcggccg gagaccccgg tcggcgccca catcccaggc gcgaccaacc ctccctttat
     3841 cctggtgaga aaacagtgcg gcgccggttg ccccggattc cttacccccg tacccgcatc
     3901 tccgccggcc cttcggcggc ggcgggctgc ggcgcgccga ccggcagggt gatcgtaaac
     3961 gtgctccccc ggcccacccg cgaatccacc tcgatccgcc ccttgtgcag gtcgacaatc
     4021 ttgctgacga gggccagccc cagcccggtg cccgaggggc gcgaggaata gaacggcgtg
     4081 aagatcttcg ccacattctc cggggcgatc ccgcaagcgc ggtcgcggac ccggatagcc
     4141 gtttccccgt tccccgccgc cgcgtcgatc tcgattttcc cggccgcccc ggagtaggcg
     4201 ttggctgcgt tctcgatcag gttgagaagc gcctggcgca agaggagcgg atcggcggga
     4261 atcacgacct cggggatttc gcccaccgca atttcgaccc gcggatacgc ttcccgcgtg
     4321 gcaaaactcg ccgccgcctg gccgatcagg tcgcgcagcg ggaccgcctg gagatccgcc
     4381 tgcagcgggc gggcgaattc caggaaccgc cgcacgagcg attcggcctg cgccgcctcg
     4441 tccgcgagct ccgccaccgc cggttcctcc accccccgct tgcccagccg ccgcttcaga
     4501 aggttcaggt agccggcgat cgcccccagc gagttgcgca actggtgcgc aagcccgccc
     4561 gacatttcgc cgagcgccgc cagccgcaac cgccgttcga gctgttcccg caactgagtg
     4621 atctcggtcg cgtcattgat cagcagcgcc gcgccgatcg gctccccttc tccgtcccac
     4681 accgtcgagg ccgcaatccc cagcaccggt gtatccggca gccccgccgg cgtcacctcc
     4741 cgatactccc cgcccgcttc gtcgacaagc ggccccgcga tagcctctgc cagttcccgg
     4801 ggcatcagct ccgcgtggaa ccgcccgcgc gcctcgcctt cccgcaggcc gagaatccgc
     4861 tccgccgccg cattgatcgt ctggatcctg
//
LOCUS       DAOOKS010000038         4886 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_20835_length_4886_cov_3.407783, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000038 DAOOKS010000000
VERSION     DAOOKS010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4886
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20835_length_4886_cov_3.407783"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..945)
                     /locus_tag="PK112_01190"
     CDS             complement(<1..945)
                     /locus_tag="PK112_01190"
                     /inference="COORDINATES: protein motif:HMM:NF013179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HPC10694.1"
                     /translation="MRQRRMLWQLFPSFVLVVALAAGGVYLYTRQAYRTAHYHEAVHD
                     LEMRARLAERVITPYLDAHDNDALDSLVVALGRAGRTRFTVVGADGRVLADSEEDPKH
                     MEQHRFRPEIAAALRGEMMASRRYSSTLQRHMLYTAVPVLADDRVVGVVRTAIPEDAA
                     EGGIGTVTGTLAGDVVVGGLLLVLLAAVISRLVARSVVRPIGDLRAGADRFARGEFDY
                     ALPAAGHQEIAGLAQAMNEMAAQLHERMEAIGRRRNEQEAILAAMVEGVLAVDTSERI
                     LTLNAAAARLLRIRPEEAVGRSVPEVVRNSELHRLVSET"
     gene            complement(945..1634)
                     /locus_tag="PK112_01195"
     CDS             complement(945..1634)
                     /locus_tag="PK112_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPC10695.1"
                     /translation="MAKEPILVIEDEQDIQELIAYNLAREGYRVVRAVTGEDGLACAR
                     REPPALVLLDLMLPGVDGLEVCRQLKHSPATRAIPIVMLTAKGEESDIVAGLEMGADD
                     YIAKPFSPKVLIARIRAVLRRKATGPEDAAARLAIHGFIIDPGRHEVSVDGRPVQLTH
                     TEFQILHLLARRPGWVYTRYQIVNQVRGEDVIVTDRSVDVQIVGLRKKLGAAGRYIET
                     VRGVGYRLREN"
     gene            complement(1773..3137)
                     /locus_tag="PK112_01200"
     CDS             complement(1773..3137)
                     /locus_tag="PK112_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPC10696.1"
                     /translation="MTVRILLADDDASLRRVLQFKLERQGFAVTAAGDGAEALDRLTR
                     ERFDLLLSDIRMPRLSGIELLERVRGVQPDLEVIFISAHAEVAQAVQAVKLGAFDYLT
                     KPFEDEQLFVAIDKALEHRRLAEENRLLRGRLRGRDVMRKLVGVSRPFREMMALVEKI
                     APTDATVLIAGESGTGKEMIARAIHYGSHRADRELVVVNCAAIPRDLIESELFGHVRG
                     AFTGAVRDKRGKFELADRGTLFLDEIAELSIDLQAKLLRAIQEKTIEPVGAEKPVEMD
                     VRLIAATNVDLKERAARGRFREDLYYRLNVIPLRVPSLRERREDIPILVKEFARAFAP
                     SAPLAVDAELMARLAAYAWPGNIRELENLIERMAILRRGEVLTTADLPPDFGRDVGPA
                     EARPAAGDPEPERTLPETEKQLIIKALTRTGGNRSRAALQLGIPRHVLVYRMKKYGLH
                     GPDA"
     gene            complement(3134..4216)
                     /locus_tag="PK112_01205"
     CDS             complement(3134..4216)
                     /locus_tag="PK112_01205"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPC10697.1"
                     /translation="MAGPRSGSLTESLPERTRYRLAIIGGIILVTVSIHYGWVLEPIF
                     GPQHWIHAIHGRLCYIPIVLAASWFGLRGGLGAAAAISLLVLPFIFVNDLGAHNTAGE
                     VTEIVFYFALGGLIGGIVDRERRVRRRQQETELALERSQRQALVGRMASTVAHEIKNP
                     IASIKGAVEIICDKDTPEADRAEFREIAVSEIRRIDGTLRDFLEFGRPREPHLAPMNL
                     SETVAAGLRQIEAPAAHAGVRLVRHIQPDVAIVGDREKIHQVMLNLLLNALEASAPGG
                     AVRVAVESEAGGARLTIADGGHGIPPEAVGRIFDPFFTTKSSGSGLGLAVARQIVESH
                     GGSIAVESTVGAGTTFTLRLPAGEVR"
     gene            4473..>4886
                     /locus_tag="PK112_01210"
     CDS             4473..>4886
                     /locus_tag="PK112_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10698.1"
                     /translation="MKSRVLHAPTSWFIVALLLVLSISGASRGAEPALADTVLPASPA
                     LDDYLRVALNRSPDVRRAYESWRAEAAQTGYAGTLPDPMLMFEYMVDPKQPRLGARQE
                     IPWLGGLRAEKAMAAAAARAAYDRCEAARLAVIYRT"
BASE COUNT          774 a         1697 c         1594 g          821 t
ORIGIN      
        1 cgtctccgag accaggcggt gcagttcgct gttgcgcaca acctcgggca cgctccgccc
       61 gacggcctcc tcgggccgga tgcggagcag ccgggcggcg gcggcattca gggtgaggat
      121 acgttcggag gtatcgaccg ccaggactcc ctcgaccatg gcggcgagaa tggcctcctg
      181 ttcgttgcgc cggcggccga tcgcctccat gcgctcgtgg agctgggcgg ccatttcgtt
      241 catcgcctgg gcgagcccgg cgatttcctg atgcccggcc gccggaaggg catagtcgaa
      301 ctcgccgcgc gcgaaccggt cggcgccggc gcgcaggtcg ccgatcgggc gcaccaccga
      361 ccgggcaacg aggcggctga tcacggccgc gagcagcacc aacagcagtc cgccgacgac
      421 cacatcaccg gccagcgtcc cggtcaccgt cccgatcccc ccttcggccg cgtcttccgg
      481 gatggccgtc cgcaccacgc cgaccacccg gtcgtcggca aggaccggca ccgccgtgta
      541 cagcatgtgg cgctggagcg tgctgctgta gcgccgggag gccatcatct ctccgcgcag
      601 ggcggcggca atctccgggc gaaaccggtg ctgctccatg tgtttcggat cttcctcgct
      661 gtcggccagc acgcggccgt cggcccccac gaccgtgaac cgcgtccggc cggcgcggcc
      721 gagcgcgacc acgagcgaat cgagagcatc gttgtcgtgc gcatccaggt agggggtgat
      781 gaccctctcc gccagccgcg cccgcatctc cagatcgtgg acggcttcat ggtaatgggc
      841 ggtccggtaa gcctgccgcg tgtagaggta gacgcccccc gccgccagcg ccacgaccag
      901 tacaaacgag gggaacaact gccacaacat ccgccgctgc cgcatcagtt ctcccggagg
      961 cggtagccga cgccccgcac ggtttcgata taccggccgg ccgcgcccag tttcttgcgc
     1021 agaccgacaa tctgcacatc gaccgaccgg tcggtgacga tcacatcctc gccgcgcacc
     1081 tggttgacga tctggtaccg cgtgtagacc catccgggcc gccgcgcgag gaggtgcagg
     1141 atctgaaatt cggtgtgcgt gagttggacc gggcggccgt cgacgctcac ctcgtgccgc
     1201 ccggggtcga tgatgaagcc gtgaatggcc agccgcgccg cggcgtcctc cggcccggtc
     1261 gccttgcgcc ggagcactgc ccggatgcgc gcgatgagca ctttggggct gaacggcttg
     1321 gcgatatagt catcggcccc catttcgagc ccggcgacga tgtcgctctc ttcccccttg
     1381 gcagtgagca tgacaatcgg gatcgcgcgg gtggcggggc tgtgcttcaa ctggcggcat
     1441 acctccaaac cgtcgacgcc gggcagcatg aggtccagca acacgagcgc cggcggctcc
     1501 cggcgggcgc aggccaaacc gtcttcgccg gtcaccgccc gcacgacgcg gtacccctcg
     1561 cgggcgaggt tgtaggcaat cagctcctgg atgtcctgtt catcttcgat gacgagaatc
     1621 ggttccttcg ccatggcgcc tctccaccgg ctgcgacggt cgctttccgc actaaggtaa
     1681 aacgcctccc ccggtaagga aaccactaac tttccgttag acttgtgtga ggaacgacgg
     1741 ggccccccga ggacgcgcgg gtcgccgacc gctcaggcgt ccgggccgtg caggccgtac
     1801 ttcttcatcc ggtacaccag aacgtgcctc gggattccga gctgcagcgc ggcccgcgac
     1861 cgattgccgc cggtgcgggt cagcgccttg atgatgagtt gcttctccgt ctccggcagg
     1921 gtgcgctccg gttccgggtc gcccgcggcg gggcgggcct cggccgggcc gacgtcgcgg
     1981 ccgaaatccg gaggcaaatc cgcggtcgtg agcacctcgc cgcgccggag gatcgccatg
     2041 cgctcgatca ggttttcgag ttcgcggata ttgcccggcc aggcgtacgc cgccagccgc
     2101 gccatgagct cggcatcgac cgccagcggc gcggaggggg cgaaagcgcg cgcgaactcc
     2161 ttgacgagaa tggggatatc ctcgcgccgc tcgcgcaggc tcggcacccg gaggggaatg
     2221 acgttgagcc ggtagtagag atcctcccgg aatctccccc gggcggctcg ctccttgagg
     2281 tccacattgg tcgccgcgat caggcggaca tccatctcga ccggcttttc cgcgccgacc
     2341 ggctcgatcg ttttctcctg aatcgcccgg aggagcttgg cctgcaggtc gatcgacaac
     2401 tcagcgattt catcgagaaa gagcgtcccc cggtcggcca gctcgaattt cccccgcttg
     2461 tcgcgcacgg ccccggtgaa cgccccccgc acgtgcccga acagctccga ttcgatcaga
     2521 tccctgggga tggcggcgca gttgacgacc accaactccc ggtcggcccg gtggctgccg
     2581 tagtggatcg cccgcgcgat catctccttg ccggtgcccg actctcccgc aatcagcacc
     2641 gtcgcatcgg tcggcgcgat tttctccacc agcgccatca tctcccggaa ggggcgggag
     2701 acgcccacga gtttccgcat tacgtcgcgc ccccggagtc gtccccgcag gaggcggttt
     2761 tcttcggcga gccggcgatg ctcgagggcc ttgtcgatgg ccacgaacaa ctgctcgtcc
     2821 tcgaacggct tggtgagata gtcgaaggca ccgagcttga cggcctgcac cgcctgggcg
     2881 acctcggcgt gggcgctgat gaaaatcacc tcaagatccg gctgtacgcc gcgtacccgc
     2941 tcgagcagtt cgatcccgga cagccggggc atcctgatgt ccgagaggag caggtcgaaa
     3001 cgctcccggg tgaggcggtc gagagcctcg gcgccgtcgc cggccgccgt gaccgcgaac
     3061 ccctgccgct ccagcttgaa ctggaggacg cggcgcagtg aggcgtcgtc gtcggccaaa
     3121 agaatgcgca ccgtcatcgc acctcccccg cgggcagccg gagcgtgaac gtcgtccccg
     3181 ccccgaccgt gctctcgacc gcgatcgacc cgccgtggct ttcgacgatc tgcctggcca
     3241 cggccagccc gagaccgctg cccgaggatt tcgtcgtgaa gaacgggtcg aaaatgcgcc
     3301 cgactgcctc cggcgggatg ccgtggccgc cgtccgctat cgtgaggcgc gcgccgccgg
     3361 cctcggattc cacggccacg cgcacggcgc ctcccggggc ggacgcctcg agcgcattga
     3421 gcagcaaatt gagcatgacc tggtggatct tctcccggtc gccgacgatg gcgacgtccg
     3481 gctggatgtg ccgcaccagc cggacgccgg cgtgggccgc cggggcctcg atctggcgca
     3541 gtccggccgc caccgtctcc gacagattca tcggcgccag gtgcggctcg cgcgggcggc
     3601 cgaactcgag gaaatcgcgg agcgtcccgt cgatccgccg gatctcgctc accgcgatct
     3661 cgcggaactc cgcccggtcc gcctccggcg tgtccttgtc gcagatgatc tccaccgccc
     3721 ccttgatgct ggcgatcggg ttcttgatct cgtgggccac ggtcgaagcc atccggccca
     3781 ccagcgcctg gcgctgcgac cgttcgagcg cgagttctgt ctcctgctgg cggcggcgga
     3841 ccctgcgctc gcggtcgacg atcccgccga tcaggccgcc gagggcgaag tagaagacga
     3901 tttcagtgac ctctccggcc gtgttgtgcg cgccgaggtc gttgacgaag atgaacggga
     3961 gcacgagcag cgagatcgct gccgccgccc ccaacccccc ccgcaggccg aaccaggagg
     4021 cggccaggac gatcgggatg tagcacagcc gtccgtggat ggcatggatc cagtgctggg
     4081 gaccaaaaat cggctccagc acccagccgt agtgaatgct gaccgtaacc aggatgatgc
     4141 cgccgatgat cgccagacgg tagcgcgttc tctcaggcag agattcagtg agcgagccgg
     4201 agcgcgggcc cgccaaaggg gagctttccg gatgggcagg cgtcttctgc acgtttcctc
     4261 ctgaaaacgg tatcaccggc ggattggccg gcttgtgatc gaccgatcca tacaccaact
     4321 tatagcctca ttcggcattc ttgttaacaa tattcttcac ccgttgaata atattcttca
     4381 atgacttcat tcgtcagcca tacattcaaa tgttaagttg ctgctaatca taccattatc
     4441 aggtcggcac gtcggttgtt ttgtgagttg gcatgaagag ccgagtactc catgctccca
     4501 ccagttggtt catcgtggcg ctgctgctcg ttctatcgat ctccggagcg tcccggggcg
     4561 ccgagccggc actggccgac acggttctgc cggccagccc ggctctcgac gattaccttc
     4621 gggtcgccct caaccggagt cccgatgtcc gtcgggccta tgagagctgg cgcgccgaag
     4681 cggctcagac gggttacgcg ggaacgctgc ccgacccgat gctgatgttc gagtacatgg
     4741 tcgatcccaa acagccgcgg ctcggggcgc gccaggagat tccgtggctt ggcgggcttc
     4801 gggccgagaa ggcgatggcg gcggctgccg cccgggcggc gtacgaccgc tgcgaggcgg
     4861 cgcgcctggc tgtgatctac cgcacg
//
LOCUS       DAOOKS010000039         4884 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_20850_length_4884_cov_3.338786, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000039 DAOOKS010000000
VERSION     DAOOKS010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4884
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20850_length_4884_cov_3.338786"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2897)
                     /locus_tag="PK112_01215"
     CDS             complement(<1..2897)
                     /locus_tag="PK112_01215"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPC10699.1"
                     /translation="MIPHWTGATAGKFLLFLALAAAFIGLTGSSQPGPGVAQPPGAIP
                     PNANAGTEGPDTTFRPYVPGEVVVRLFSDSISVDSINTLFGTTVKEYFGQVNIYILAT
                     ADPAADIEALVENLRPLPGVIFAHPNYVVSPLQSVQGSFPFSDADNVGSYPEQEAALR
                     LRLSQAHALATGAGVKVAVVDGGVNFAHPVLAGAAVSGWDYVDGDADAFDEPGGRNSG
                     HGTFVAGVVHLTAPGAQIIAYRVADTSGRSNGDRVAEAILRAIDDGCRVVNLSLVMTN
                     VHEVLRNTLAYARSRDVLVVAAAGNERLEVPTYPASDPNVVAVAAVDTLDRLAEFSRY
                     GNHIDLCAPGEWIYAPYFDMYAWWSGTSFAAPFVAGQAALKVSQAGGALDWRSLHASL
                     IATAENIYPANPGFFGKLGAGLANPLQALQDTVGIDTASVDPGVLALTLPPNTAVQRC
                     FNLYSTNPNSVFNATVLGGNVAFTTITADSVLGANATICFTLSSSGLSEGIYADTIQF
                     DISGVSNSPIFVPLSLYVSDTAVISQDAAVRPTGYSFWLPEGWVQTIDACAYLTSTNA
                     PAAFTVRRIDTSAASFVEILSPSGMTNDSVCILLHPAGLAPGVHLDTLVYRVSGVTYD
                     LYQPVSLTIGDQVDSAAVSPMYFYFTSPPGGPPQEGRAYLRSTNAPSAYYLETAGGSD
                     WLHVPDSAGVTNDSISFTIAPAAGVVPGLYTDTLYCHVAGAVNTPAVYVTLEVQDSTA
                     GATATAHPTYFEFVHAPWAGGPAQAGVFLLASTNAPASYSVEAALGVSWLHLADSIGT
                     TNDSVRFTVDGAGLAPGFYWDSLICRVTGVEIPAAVQVLLTVLDDTGGTGPSAYASPS
                     WFSFASDPQTPPYAGSFVLSSSNAPAAFTAAVMFSSPWLHVIDAAGTTNDSVRFTVDA
                     AGLAPGFYSDSIACVVAGVPDSVLVPVQLSVAPEDTLGGDSAVAIPASFSLT"
     gene            complement(2967..>4884)
                     /locus_tag="PK112_01220"
     CDS             complement(2967..>4884)
                     /locus_tag="PK112_01220"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HPC10700.1"
                     /translation="LCVLDRAETYLSLNLLPDARDTARRAAMLARRLGLRYEQAKAEF
                     FRGKALAALGRMKAGRTAFRTAAAGFRREGNEGFLAAVRVAEAVAGESGRARARALAA
                     LRERMPQGRLPLWEALCDLEILSAAPEDGGARRRLAANRAVETMPHVRAQYVTLLGDS
                     EARRGRRRAATGHWRRAAEILTAVRAQLPPLDLRAAFSERHAEPYEKLIACEWEAHPS
                     QAAAWLERFHTAGIWATEDARLDDPARTQAEQSLASLARYVAAAAQRSDGRRVRGGGA
                     APATSLRRQLEEQIRTDLARLERRRGSAAERLDRLGAEIAAASRTMRLVQLHAVSGDI
                     IGLIHEEGRPRGCRLEGAAPAVERLLQRWRFFVERAPYRTAGETAADLADERRLLARL
                     AETLWRPLDVGGPQPVLIVADGALANLPWAALPVGGRPLIDRHPLVMAPSVRHHLAAA
                     RQRTQSRETRIFVGAAGLGAAGAEYAPLLADERRQTTVHNPCGRADWPVDSEADVWHF
                     TGHAHFRSDNPFYSFLELADGPLFAADFRLRRNTVNLAVLAACRTGLSSFLPGEESTG
                     LIRALLEMGARNVLASHWAVADASTARWIERFYGALAEGSGPAAAVQRAAADAREEYP
                     SAYDWAGFSLFGAG"
BASE COUNT          819 a         1785 c         1596 g          684 t
ORIGIN      
        1 gtgagactga aactcgcggg aatggccacc gccgaatccc cgcccagcgt gtcctccggc
       61 gctaccgata gctggaccgg cacgagcacc gaatcgggca ccccggcgac cacgcaggcg
      121 atgctgtcgc tgtagaaacc gggggccagc ccggccgcgt cgaccgtgaa ccggaccgag
      181 tcgttggttg tgccggccgc atcgatgacg tgcagccacg gcgaggagaa catgaccgcg
      241 gccgtgaacg ccgccggggc gttcgacgac gacagtacga acgatccggc gtagggcggg
      301 gtctgcggat cgcttgcgaa actgaaccag gagggactcg cataggcgct tggccccgtg
      361 ccgcccgtgt catccagcac cgtgagcagg acctgcaccg cggccggtat ctccaccccc
      421 gtcacgcggc agatgaggct gtcccagtag aagccggggg ccaaacccgc gccgtccacg
      481 gtaaaccgga ccgagtcgtt ggtcgtcccg atcgagtccg ccaggtgcag ccacgacact
      541 cccagggccg cctcgaccga gtaggacgcc ggggcattgg tcgacgccaa gaggaacacg
      601 cccgcctgcg ccggcccgcc cgcccaggga gcatggacaa actcaaagta ggtggggtgg
      661 gcggtggccg tcgctccagc cgtcgaatcc tgcacctcca gcgtgacata caccgccggc
      721 gtgttcaccg cccccgccac gtggcagtaa agcgtgtcgg tgtagagccc cggaacaacc
      781 ccggccgccg gcgcgatcgt gaatgatatg gaatcgttcg tcaccccggc cgaatcgggc
      841 acgtgcagcc agtcggaacc gccggccgtc tcgagatagt acgccgaggg ggcgttggtc
      901 gaccggagat atgcgcgccc ctcctgcggc gggccgcccg gcggcgacgt gaagtagaag
      961 tacatcgggc tgaccgccgc cgaatccacc tggtcgccga tcgtcagact caccggctgg
     1021 tagaggtcgt acgtcacccc gctcacccgg tacaccagcg tgtcgaggtg cacgcccggc
     1081 gccaggccgg ccgggtggag gaggatgcac acgctgtcgt tggtcatgcc ggacggggag
     1141 aggatctcca cgaacgacgc cgcgctcgta tcgatccgcc gaaccgtgaa cgcggccggc
     1201 gcgttggtgg aagtcaggta cgcgcacgcg tcgatcgtct gcacccagcc ctccgggagc
     1261 cagaacgagt atccggtcgg cctcaccgcc gcgtcctgtg agatgacggc tgtgtcgctc
     1321 acgtacaggc tcagcggcac gaagatcggg ctgttcgaca cgccgctgat gtcaaactgg
     1381 atcgtgtcgg cgtagatccc ctcgctcagc ccggacgacg agagagtgaa gcagatggtt
     1441 gcgttcgcgc ccaacaccga gtccgccgtg atcgtcgtga atgcgacatt accgcccagc
     1501 acggtggcgt tgaagaccga attggggttg gtcgagtaca ggttgaaaca gcgctggacc
     1561 gccgtgttcg ggggcagcgt gagcgccagc acgccggggt ccaccgaggc cgtatcgata
     1621 ccgaccgtat cctgcagcgc ctggagcggg ttcgccaaac cggcgcccag tttcccgaaa
     1681 aagcccgggt tggccgggta gatgttctcg gcagtcgcga tcagcgaggc gtggaggctg
     1741 cgccagtcga gcgcgccgcc ggcttgcgaa accttgagcg ccgcctggcc cgcaacaaac
     1801 ggagcggcga aactcgtccc gctccaccag gcgtacatgt cgaaatacgg ggcgtagatc
     1861 cattcccccg gcgcacagag atcgatgtgg ttgccgtagc gcgaaaattc cgccagccgg
     1921 tcgagtgtgt cgaccgccgc gaccgccacc acgttcggat cggaggccgg ataggtcggc
     1981 acctccaacc gctcgttgcc ggcagcggcc acgaccaaca catcgcggct gcgcgcatag
     2041 gcgagcgtgt tccgcagcac ctcgtggacg ttcgtcatca cgaggctgag attgaccacc
     2101 cggcagccgt cgtcgatcgc ccgaaggatc gcctccgcca cccggtcgcc gttgctccgc
     2161 ccggaggtgt cggccacccg gtaggcgatg atctgcgccc ccggcgcggt cagatggacc
     2221 acccccgcca cgaacgtgcc gtggccgctg ttgcggcccc ccggctcatc gaacgcatcg
     2281 gcgtcgccgt cgacatagtc ccagcccgaa accgctgctc ccgcgagcac cgggtgggcg
     2341 aaattcacgc cgccgtcgac caccgcgacc ttgaccccgg cccccgtcgc cagcgcgtgc
     2401 gcctgcgaaa gacgcagccg cagagccgcc tcctgctccg ggtaggaacc cacgttgtcg
     2461 gcatcggaga aggggaaact cccctgcacc gactgcagcg gcgacaccac gtagttgggg
     2521 tgtgcaaata tcacccccgg caggggccgc aggttctcca cgagagcctc gatatcggcc
     2581 gccgggtcgg ccgtggcgag gatatagatg ttgacctggc cgaaatactc cttcaccgtc
     2641 gtgccgaaca gcgtgttgat cgaatcaacc gagatcgagt cgctgaacag cctcaccacc
     2701 acttcgccgg gaacatacgg ccggaaagtc gtgtccggac cctccgtccc ggcgttggcg
     2761 ttgggcggaa tcgcgcccgg cggctgtgcc acgcccggcc cgggttgcga cgacccggtc
     2821 aggccgatga aggcagccgc cagcgcgaga aacagcagaa acttccctgc ggtggccccg
     2881 gtccagtgcg gtatcatagg aacgcctcct ttatccaaga agctgcggga catttttctc
     2941 acacccgatc tattccaggt tcggcgtcag ccggcgccga acagcgaaaa cccggcccaa
     3001 tcgtacgccg agggatactc ttcccgcgcg tccgcggctg cccgctgcac ggccgcggcg
     3061 ggtccgctcc cttcagcgag cgccccatag aaccgctcga tccagcgcgc ggtcgaggcg
     3121 tccgccaccg cccagtggct ggccagcacg ttgcgcgctc ccatttccag cagcgcgcga
     3181 atcagcccgg tcgactcctc gccgggcaag aacgacgaca acccggtccg acaggccgcc
     3241 agcaccgcca ggttcaccgt gttccgccgc aggcgaaaat cggcggcgaa cagcggcccg
     3301 tcagcaagct ccaggaagga gtaaaacgga ttgtcgctgc gaaaatgcgc atggccggtg
     3361 aaatgccaga catcggcctc cgaatcaacc ggccagtcgg cgcgaccgca gggattgtga
     3421 accgttgtct gcctgcgctc atcggccagc agcggggcat actccgcgcc cgccgcgcca
     3481 agccccgcgg cgccgacaaa tatcctggtc tcccgcgact gtgtccgctg ccgcgcggct
     3541 gcgaggtgat gccgcacgct cggcgccatc accagcggat gccggtctat caatggcctt
     3601 cccccgaccg gcagcgccgc ccaggggaga ttcgccaggg cgccgtcggc tacgatcagc
     3661 actggttgcg gcccgcccac gtccagcggc cgccagaggg tctcggccaa ccgggcgagg
     3721 agccgccgtt catcggcgag atcggccgcg gtttcaccgg ccgtgcgata gggcgcgcgc
     3781 tcgacaaaaa accgccatcg ctggaggagg cgctcgaccg ccggcgcggc gccctccagt
     3841 cggcagccgc gcggccgccc ctcctcgtgg atgaggccga tgatgtcgcc ggagacggcg
     3901 tgcaactgga caaggcgcat ggtccgggag gccgcggcga tttccgcgcc cagccggtcg
     3961 agccgctccg ctgcgctccc gcgcctgcgc tccaaccgcg caaggtcggt gcgaatttgc
     4021 tcctcgagct gccgccgcaa cgatgtcgcc ggcgccgccc ccccgccgcg cacccgccgg
     4081 ccgtcggatc gctgcgccgc cgccgccacg taccgcgcca gcgaggccag cgactgctcc
     4141 gcctgcgtcc gcgccggatc gtcgagccgc gcatcctcgg tcgcccagat gccggccgtg
     4201 tgaaagcgct ccagccaggc cgccgcctgc gagggatggg cctcccactc gcacgcgatc
     4261 aacttctcgt acggttccgc gtggcgctcg gagaaagccg cccgcaggtc aagcgggggg
     4321 agctgggcgc gcacagccgt caggatttcg gcggcccggc gccagtggcc ggtcgccgcc
     4381 cggcggcggc cccgccgcgc ctcgctgtcc cccagcagcg tcacatactg cgcgcgcacg
     4441 tgcggcatcg tctcgaccgc gcggttggcg gccagccgcc gccgcgcccc tccgtcctcc
     4501 ggcgcggcgc tgagaatctc cagatcgcac agcgcctccc agagcggcag ccgcccctgc
     4561 ggcatgcgtt cccgcagcgc cgccagcgcg cgggcgcgcg cccgccccga ttctccggct
     4621 accgcctccg ccacccgcac cgcggcgagg aaaccttcgt ttccctctct tcgaaagccc
     4681 gcggccgccg tccggaaagc cgtccggccg gccttcatcc ggccgagcgc tgccagcgcc
     4741 ttgcccctga aaaactccgc tttggcctgc tcgtagcgca aacccagccg gcgggccagc
     4801 atcgccgccc ggcgcgccgt gtcgcgcgca tccggcaaaa gattcaaact caggtaggtc
     4861 tctgcgcgat caagtacgca gagc
//
LOCUS       DAOOKS010000040         4856 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_21016_length_4856_cov_3.759217, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000040 DAOOKS010000000
VERSION     DAOOKS010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4856
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21016_length_4856_cov_3.759217"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2555)
                     /gene="alaS"
                     /locus_tag="PK112_01225"
     CDS             complement(<1..2555)
                     /gene="alaS"
                     /locus_tag="PK112_01225"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HPC10701.1"
                     /translation="MVPSSPVIPFDDPTLLFTNAGMNQFKDVFTGQRTLPYARATSSQ
                     KCIRAGGKHNDLDNVGFTARHLTFFEMLGNFSFGDYFKEEAIAFAWEWVTKDLGLDPQ
                     RLSATVYETDDEAFALWAKIAPALKDGRILRFGKKDNYWSMGDVGPNGPCSEIHYDRG
                     AKYGTGPEDRVNGESDRFVEIWNLVFMQYETLADGAVVALPKPSVDTGAGLERIAAVI
                     AGANSVFEIDLFRRIIEAIADLTGAGPRARSASHNVIADHLRALCFAIADGAGISNEG
                     QGYVLRRILRRAARHGRNLGMHRPFIHRLVPVLAAEMGDAYPEIREKQPHLENVIRTE
                     EESFGRTLETGLELFERVARKVAGEGSAVVPGEEVFRLYDTYGFPYDLTEIIAAERGL
                     RLDREGFDRAMERQRTQSKAGARFAAQVSSLNEVLDAAGLRAAPTVFARDRFEAAASI
                     QHVFPWPGGDPRRVGIILDTTPFYTEAGGQVDDTGVIVGPGAAVRVEAVHGYRDWSVH
                     EGVVTEGSPADLRAGDGVTAKIDAERRWAIMRNHTVTHLTHAALRTVLGPHIKQSGSY
                     VGPDRMRFDFSHHRPMTPEEIREVERLVNEQILRGTAVQTEIMDIDAARRTGAMALFG
                     EKYGDRVRVVSVPGFSKELCGGTHVQNVAQIGPFFITLETGIASGVRRLEAITGTAAI
                     GYMLDAKQFRSEAAQAVGRSEKDALEGVRQLRENCAELQRELKRVKAEMFSGSPRAVG
                     AEEQIGAVTLATHDFGDTDRDIMAGWIDRQKDRPDPVVAVALGKVDGKQVYVASASSE
                     AVSRHRVNIGALSKEILPQFGGRGGGKPTFAQGSVADEAEAARVFAAVRASLTA"
     gene            complement(2611..3261)
                     /locus_tag="PK112_01230"
     CDS             complement(2611..3261)
                     /locus_tag="PK112_01230"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HPC10702.1"
                     /translation="MDERISLIDIRLVGSRAEIHLSTHERPLVLSPETVHRYRLVKGT
                     VLTRSQAAQLETEAARFAVASEATRLLARRPHSAGELRQKLLRQFDAELVGAAIRDFT
                     ARGVLDDAQYAHQLADSLVRRKPCGRGYVIAFLESRRIERALAAEAADAVLSEHSPEE
                     LAERALRAKWREFGQFDLETARRRAYNYLARRGFPFDIARAAVQTLARESTDGGND"
     gene            3608..4474
                     /locus_tag="PK112_01235"
     CDS             3608..4474
                     /locus_tag="PK112_01235"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HPC10703.1"
                     /translation="MDLDLQGNIERFTLPEILQLIASGRKSGTLGIQKADSIVMVYFR
                     EGNIIYGYGPRETYHLGQLLKERGLITAEQLEEAVRMQAVTDNSRRLGEILITRGFID
                     RADLQDVVRRQVESLLYSLLSWTSGSFKFYEDQFPTEEEITVDLSVENVILEGLRRID
                     EMNLIRETLPNLEAVYTISAAQGGRARNVALHADEWNIMALVDGHRTLNEVCRLSPLG
                     RDETLRKLAQLKLAGLITKTDRPIEPPAPAADLEKMVGRLAGLLESYMTEKAAPRPAE
                     RRITQSLLEQVD"
     gene            4471..>4856
                     /locus_tag="PK112_01240"
     CDS             4471..>4856
                     /locus_tag="PK112_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10704.1"
                     /translation="MSQIHTLKSLEQVLETFLERVVAYKQPRLQVLEGINRLDDLART
                     PDPGTDFTEQVGEWFAAHNRLLDQKQLRTADLNRIGGILAEIRSRLDLGGDSPPAAHK
                     ISSEIERWRSGAPTAVPPRITLARGP"
BASE COUNT          808 a         1645 c         1557 g          846 t
ORIGIN      
        1 gcggtgaggc tcgcccgcac cgccgcgaac acccgggccg cctcggcttc gtcggccacg
       61 ctcccctggg cgaaagtcgg tttgccgccg ccccggcctc cgaactgcgg caggatctct
      121 ttggagagcg ccccgatgtt cacccggtgc cggctgaccg cctcgctcga ggccgacgcc
      181 acgtacacct gcttgccgtc gactttcccc agcgccaccg cgaccaccgg atcgggccgg
      241 tctttctgac ggtcgatcca cccggccatg atgtcgcggt cggtgtcgcc gaaatcgtgg
      301 gtggccagcg tcacggcgcc gatctgctcc tccgcgccca ccgcgcgcgg cgagccggag
      361 aacatctccg ccttcacccg cttcagttcg cgctgcagtt cggcgcagtt ttcgcgaagc
      421 tgccgcacgc cctcgagggc gtctttttcg ctccgcccga ccgcctgggc cgcctcgctg
      481 cggaactgct tggcatcgag catgtagccg atcgcggccg tgccggtgat cgcttcgagg
      541 cgccgcacgc ccgaggcaat gcccgtctcc agggtgatga agaacggtcc gatctgggcg
      601 acgttctgca cgtgggtgcc gccgcacagc tccttggaga atcccgggac cgacaccacc
      661 cggacccggt cgccgtattt ctctccgaac agcgccatcg ctcccgtccg ccgggccgcg
      721 tcgatatcca tgatctcggt ctgcaccgcc gtgccgcgaa ggatctgctc gttgaccagc
      781 cgttcgacct cgcggatctc ttccggcgtc atcggccggt ggtgggagaa gtcaaagcgc
      841 atgcggtccg gtccgacgta cgatcccgac tgtttgatgt gcgggccgag caccgtccgc
      901 agcgcggcgt gggtgaggtg ggtgacggtg tggttgcgca tgatggccca gcgccgctcg
      961 gcgtcgattt tcgccgtaac cccgtctccg gcccgcaggt cggccggcga gccctcggtc
     1021 accacccctt cgtgcaccga ccagtcccgg tagccgtgca cggcctcgac ccgcacggcc
     1081 gcgcccgggc cgacgatcac gccggtatcg tccacctggc cgccggcctc ggtgtagaac
     1141 ggcgtggtgt cgaggatgat cccgacccgc cggggatcgc cgcccggcca ggggaagacg
     1201 tgctggatgg aggcggccgc ctcgaaccgg tcgcgcgcga acacagtcgg cgccgcgcgc
     1261 agaccggcgg cgtcgagaac ctcgttgagc gacgacactt gcgcggcgaa gcgcgccccc
     1321 gccttcgact gcgtgcgctg ccgctccatc gcccggtcga acccttcccg gtccagccgc
     1381 aggccgcgct cggcggcgat gatctcggtc aggtcgtacg ggaacccgta ggtgtcgtac
     1441 aggcggaaca cttcctcgcc cggcaccacc gcgctcccct cccccgcgac tttgcgcgcg
     1501 acccgctcga acagctccag ccccgtctcg agcgtgcggc cgaacgactc ctcttcggtc
     1561 cggatgacgt tttcgaggtg cggctgcttt tcgcggattt ccgggtaagc gtcccccatc
     1621 tcggccgcca gcaccgggac gagccggtgg atgaagggtc ggtgcatgcc gagattgcgt
     1681 ccgtgccggg ccgctcgccg gaggatgcgc cgcaggacgt acccctggcc ttcgttcgat
     1741 atccccgcgc cgtcggcgat ggcgaaacag agggcgcgca ggtggtcggc gatgacgttg
     1801 tgcgaggcgg agcgggcccg ggggccggcc ccggtcaggt cggcgatggc ctcgatgatg
     1861 cgccggaaga ggtcgatctc gaaaaccgag ttcgcccccg cgatcaccgc ggcgatccgc
     1921 tcgagcccgg cgccggtgtc gaccgagggt ttcggcagcg ccacgaccgc gccgtcggcc
     1981 agcgtctcat actgcataaa gacgagattc cagatttcga caaaccggtc ggactcgccg
     2041 ttgacccggt cctccggccc cgtgccgtac ttcgccccgc ggtcgtagtg gatttcgctg
     2101 cacggtccgt tcggcccgac gtcgcccatc gaccagtagt tgtctttctt gccgaagcgg
     2161 agaatgcgcc cgtccttcag cgccggcgcg atcttcgccc acagcgcgaa cgcctcgtcg
     2221 tcagtctcgt acaccgtcgc ggagagccgc tgcgggtcga gcccgagatc cttcgttacc
     2281 cactcccagg cgaacgcgat ggcctcctcc ttgaagtagt cgccgaagga gaagttgccg
     2341 agcatctcga aaaaggtgag gtgccgggcg gtgaatccga cgttgtcgag gtcgttgtgt
     2401 ttgccgcccg cccgaatgca cttttgcgag ctggtcgccc gggcataggg cagggtgcgc
     2461 tgcccggtga acacatcctt gaactggttc atcccggcgt tggtgaacag gagcgtcgga
     2521 tcgtcgaagg gaataaccgg agatgacggg accacccgat ggtcgtggcg ggcgaaaaag
     2581 tcaaggaaag atcggcgaat atctgatgtc ttaatcgttg cctccgtccg ttgattcacg
     2641 cgcgagcgtc tgcacggcgg cgcgggcgat gtcgaaaggg aagccgcgcc gggccaggta
     2701 attatatgct ctgcggcggg ccgtttcaag gtcaaactgc ccgaactccc gccacttcgc
     2761 ccgcagcgcg cgctcggcca gctcttccgg cgagtgctcc gaaagcacgg cgtccgcggc
     2821 ctcggccgcc agcgcgcgct cgatccgccg gctctcgagg aacgcgatca cgtaaccgcg
     2881 cccgcagggt tttcggcgca cgaggctgtc ggccagctgg tgcgcgtact gcgcatcgtc
     2941 gagcacgccg cgcgcggtga aatcgcgaat cgccgcgccg accagctccg catcgaattg
     3001 cctcagcagc ttctggcgaa gctctccggc cgaatggggg cgccgcgcca gaaggcgggt
     3061 cgcctcgctg gcgaccgcga accgcgccgc ctcggtctcc aactgcgcgg cttgcgatcg
     3121 ggtcagaacg gttcctttga ccaaccggta gcggtgcacg gtctccggcg acagcaccag
     3181 cggccgctcg tgcgtggaca gatgaatttc ggcccggctc ccgaccaggc ggatgtctat
     3241 cagagagatt ctctcgtcca ttgttgccaa ccccggatca agccttcatt aatagcgatt
     3301 tcttcggcgg gtaaagaaaa aaagcgctgg gcgggccgca ggctgcggaa aaatccgcca
     3361 gcatccgtgt ttttatagtg cattttctca cagacatttg tacttcattg ttgacaggtg
     3421 tcgacagaac gattattgtg agttgttctg aatgatcgcg tgtaaccttt tataagcaag
     3481 caggtttccg cgaacaaaat ttgtttgaac cttaaaattt tgctgttcat aaagtaatga
     3541 ctgaggcttc gtccgcgctc accgaggacg gagccggtgg acagcccaaa catatcgggg
     3601 agcatcaatg gatcttgacc tgcagggaaa tatcgagcgg ttcactcttc cggaaatcct
     3661 tcaattaatc gcctcgggcc gcaagtccgg gactctcggg atccagaagg ctgactcgat
     3721 cgtcatggtc tatttccggg agggcaacat catttacggc tacggcccgc gcgaaaccta
     3781 ccacctgggg cagttgctca aggaacgggg actcatcacg gccgagcagc tcgaggaagc
     3841 ggtgcgcatg caggcggtca ccgataacag ccgccgcctc ggggaaatcc tcatcacccg
     3901 cggcttcatc gaccgcgccg atctccagga tgtggtccgc cgccaggtcg agagtctcct
     3961 ctattcgctc ctctcgtgga ccagcggatc gttcaagttc tacgaggatc agttcccgac
     4021 cgaggaggag atcacggtgg acctgtcggt cgagaatgtc attctcgaag gactgcggcg
     4081 gatcgacgag atgaacctga tccgggagac gctgccgaac ctcgaggccg tctacacgat
     4141 ctcggccgcc cagggggggc gcgcccgcaa cgtcgccctg cacgccgacg agtggaacat
     4201 catggcgctg gtcgacgggc accggacgct caacgaggtc tgccggctct cgccgctggg
     4261 gcgcgacgag acgctgcgga aactcgcgca actgaaactg gccgggctga tcacgaagac
     4321 cgaccggccg attgagccgc ccgcgcccgc cgccgatctg gagaaaatgg tcggccgtct
     4381 ggccggtctg ctggagagct acatgaccga gaaggccgcc ccccggccgg ccgagcgaag
     4441 aatcacccaa agcctcctgg agcaggtcga ttgagccaga ttcacaccct caagtcgctc
     4501 gagcaggtgc tcgagacttt cctggaacgg gtggtcgcgt acaaacagcc ccgcctgcag
     4561 gtgctcgagg ggatcaaccg gctcgatgat ctcgcccgga cgccggatcc cgggaccgat
     4621 ttcaccgagc aggtggggga gtggtttgcg gcccacaacc gccttctgga ccagaagcag
     4681 cttcgcaccg ccgacctcaa ccggatcggc ggcattttgg cggaaatccg cagccgcctt
     4741 gaccttggcg gggattcgcc gccggcggcc cacaagatct cctcggagat tgagcgctgg
     4801 cgctccggcg ccccgaccgc ggtcccgccc cgcattacgc tcgcccgggg cccgga
//
LOCUS       DAOOKS010000041         4794 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_21378_length_4794_cov_3.099388, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000041 DAOOKS010000000
VERSION     DAOOKS010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4794
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21378_length_4794_cov_3.099388"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /gene="mreC"
                     /locus_tag="PK112_01245"
     CDS             <1..411
                     /gene="mreC"
                     /locus_tag="PK112_01245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HPC10705.1"
                     /translation="RGSADFVRVDQPVINQYGLIGRVRSTTPRFATVQLLTDPANRVA
                     GRIERSREMGIVRYLPGRGMILDNFPIFGDIAVGDTVVSSGLGGIYPEGLVVGVVTDV
                     HRGEHAAFADVAIDPAVNFQSIEELFLLRTEGRP"
     gene            408..893
                     /gene="mreD"
                     /locus_tag="PK112_01250"
     CDS             408..893
                     /gene="mreD"
                     /locus_tag="PK112_01250"
                     /inference="COORDINATES: protein motif:HMM:NF016019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HPC10706.1"
                     /translation="MTVIPYLLYLLLVAFHEVILRDLTAIYGVTINLPVLLVLGVALY
                     KPEAAALWFGFAAGLAASAARPELSGWYALLTAGLAAAAWHVRERLNLEALLSKLLLV
                     FLGVLAYNLVAAAVGGLEGYWFHVYRLQIPGAVYTALVAWVFFLFKERRVTAQKLKAM
                     F"
     gene            912..2801
                     /gene="mrdA"
                     /locus_tag="PK112_01255"
     CDS             912..2801
                     /gene="mrdA"
                     /locus_tag="PK112_01255"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HPC10707.1"
                     /translation="MTHPHLPLELRAKAALALVIAGALFLVAGLVRLQIFKYAQMAAQ
                     SEANRLRIVPIVPRRGVVYDRDGRRIIDNRPSYTLSVVPAEEVRGVTVPNLARLLGVD
                     TLEIRKRIRRNTISVFQPAPVQRDVSFETIAVIEEQVEQFPGVGYQVEQVRQYPVGLG
                     AEVFTGYVGEVSREELERYGNDNYPLGSMIGKKGIEKQYDQLLRGREGTRYMEVSATG
                     QLLGEYRGRQRQEATPGTDLTLTIDLDLQRACGEALDTLRCGAIVAADPRTGEILAMA
                     SYPAFDANVFSGVIPESLWTAISSDTLHPLLNRPLAGLYPPGSTAKVVTVGAGLEEGL
                     ITPSSTFRSCIGGYQFGNRFFRCWEKGGHGSLVAAHAIEQSCDVYMYQAGLKLGVDRL
                     SEYYIKCGFGRPTGINLPGELKGFIPTTEWYNRTYGKSGWTRAGVLNNSIGQGEVLAT
                     PLHLLQLYCGLAGNGIVQQLHIVRRFTTPAGDELEVSPKIKFALPFSESTLAILREGI
                     ELVVQGDRGTARSLRRKEYRIGGKTGTAQNPHGNDHAWFCGVAPLEAPEIVVVAIVEN
                     AGHGSTAAAPVVGKIIDAYMKKKRDPEAFFAEQQARADSLAAARALAGLSDTAAAPAR
                     AARGN"
     gene            2801..4024
                     /gene="rodA"
                     /locus_tag="PK112_01260"
     CDS             2801..4024
                     /gene="rodA"
                     /locus_tag="PK112_01260"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HPC10708.1"
                     /translation="MLDYRQLDWKLIGAVFALSAIGVVLIMTAQHHAESEYARTFYLR
                     QLLWLVIALAAAAAVLHLPYRLFDFAAYIFYAVAVLLLVAVLMFGATRMGATRWFSFG
                     PINFAPADVAKLALIFALARFFAYSKSPLLSGQRVAVSLGLTVVPMMLILKQPDLGTA
                     LTFPVVMYALWFWSGIPPLYLLLIASPLLSLVAASNAISWSIYMAVLLAVLILFKPGR
                     QLIFSVLIVVANLAFGAVMPLVWNRLEEYQQLRIISFVDPSQDPRGAGYQIIQSKIAI
                     GSGGLWGKGFLGGSQTKLDFLPERHTDFIFSVLGEEFGLWGALIVLALFFVFFYRGIR
                     IAARSRSRFLSYVSFGGVAIIFFQFLVNIGMTLGLMPVTGVPLPFLSYGGTSLVLSWV
                     LVAFLLKADYHWQEY"
     gene            complement(4041..>4794)
                     /locus_tag="PK112_01265"
     CDS             complement(4041..>4794)
                     /locus_tag="PK112_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HPC10709.1"
                     /translation="TAGGRYYTFAELLRRDGYRVLPSGCRFTGDSLARGEILVVSNAL
                     HERNAQEWSLPTPSAFTPDEIAAVAAWVQGGGALLLIADHMPFPGAAAELAAAFGFHL
                     HNGFAVDTASPTGGTYVFRRADGTLRDHAITRGRNDRERIDSVASFTGEAFRADSAAG
                     LMVFGDGVVSLAPDTAWQFDANTAAVAVSGWCQGAVKVIGAGRVAVFGEAAMFSAQLA
                     GPNRTPAGMNSPAAPQNARFLLNVLHWLSGEL"
BASE COUNT          710 a         1753 c         1458 g          873 t
ORIGIN      
        1 cgcggctcgg ccgacttcgt ccgcgtcgac cagccggtca tcaaccagta cggcctcatc
       61 ggccgggtgc gctcgaccac cccgcgcttc gccaccgtcc agctcctcac cgaccccgcc
      121 aaccgcgtcg ccgggcggat cgaacgcagc cgcgagatgg gcatcgtccg ctacctgccg
      181 ggccgaggga tgatcctcga caacttcccc atcttcggcg acatcgccgt cggcgacacg
      241 gtcgtctcct ccggtctcgg cggaatctac cccgagggtc tcgtggtcgg ggtggtgacc
      301 gacgtccacc gaggggaaca cgccgctttt gccgacgtcg ccattgaccc ggccgtgaat
      361 ttccagtcca tcgaggagct cttcctcctg cgcacggagg ggcggccatg accgtcatcc
      421 cctacctcct ctatctgctc ctggtggctt ttcacgaggt gatcctgcgc gacctcacgg
      481 ccatctacgg cgtcacgatc aacctgccgg ttcttttggt cctcggggtg gcgctgtaca
      541 agccggaggc ggcggccctg tggttcggct tcgccgccgg gctcgcggcc tcggccgccc
      601 ggcccgaact gtccggctgg tacgccctcc tgacggcggg gctggccgcc gccgcctggc
      661 acgtgcgcga gcggctcaat ctcgaggctc tcctgtcgaa actcttactg gtgttcctcg
      721 gcgtcctggc gtataacttg gtggccgcgg ccgtgggggg gctggagggg tactggttcc
      781 acgtctaccg gctgcagatt ccgggcgccg tgtacaccgc cctcgtcgcc tgggtgttct
      841 ttctcttcaa ggagagacgg gtgacggcgc agaaactgaa agcgatgttt tgacgcggtt
      901 tcccgcaggt tatgacgcac ccgcatttgc cactggagct tcgcgcgaag gccgccctcg
      961 cccttgtgat cgcgggcgcc ctgtttctcg tcgccgggct ggtccgcctt cagattttca
     1021 agtatgccca gatggccgcg cagtcggagg ccaaccgcct ccgcatcgtg ccgatcgttc
     1081 cccgccgcgg tgtcgtgtac gatcgcgacg gccgccggat catcgacaac cggccctcct
     1141 acaccctctc cgtcgtcccc gccgaggaag tccgcggggt gacggtgccg aatctcgccc
     1201 gcctcctcgg tgtcgacaca ctcgagatcc gcaagcgcat ccgccgcaac accatcagcg
     1261 ttttccagcc cgccccggtc cagcgcgacg tctccttcga gacgatcgcc gtgatcgaag
     1321 aacaggtcga gcagttcccg ggtgtcggtt accaggtgga acaggtgcgg cagtacccgg
     1381 tcggtctcgg ggccgaggtt ttcaccggct acgtgggcga ggtgtcgcgc gaggaactcg
     1441 agcgctacgg caatgacaac tacccgctcg gcagcatgat cgggaaaaag gggatcgaga
     1501 agcagtacga ccagcttttg cgggggcggg aaggcacgcg ctacatggag gtctcggcca
     1561 ccggccagtt gctcggcgag taccggggga gacagcgcca ggaggccact cccggcaccg
     1621 atctcaccct caccatcgac ctcgatctcc agcgcgcctg cggcgaggcc ctcgacacgc
     1681 tccgctgcgg cgccatcgtc gccgccgacc cgcgcaccgg agagatcctc gccatggcct
     1741 cgtacccggc ttttgacgcc aacgtcttct ccggcgtcat ccccgagtcc ctctggaccg
     1801 ccatctcctc cgacaccctc cacccgctgc tcaaccgccc cctcgccggg ctctatccgc
     1861 ccggttcgac cgccaaggtg gtcacggtcg gcgccggcct cgaggagggc ctcatcacgc
     1921 cctcgtcgac gttcaggtcg tgcatcgggg gctaccagtt cggcaaccgg ttcttccgct
     1981 gttgggagaa agggggccac ggcagccttg tggccgccca cgccatcgag cagtcctgcg
     2041 atgtctacat gtaccaggcg ggactcaagc tcggcgtcga ccggctgagc gagtactaca
     2101 ttaagtgcgg cttcggccgc ccgaccggca tcaatctccc cggcgagctg aaaggtttca
     2161 tcccgaccac cgagtggtac aaccggacct acggcaagag cggctggacg cgcgcgggtg
     2221 tgctcaacaa ctccatcggc cagggcgagg tcctcgccac gccgctccac ctcctccagc
     2281 tctactgcgg cctggccggc aacggcatcg tgcagcagct ccacatcgtg cgcagattca
     2341 ccaccccggc gggggatgaa ctggaggtga gtccgaagat caagttcgcc ctccccttct
     2401 ccgaatctac gctggcgatt ctccgcgagg gcatcgagct ggtcgtgcag ggagaccgcg
     2461 gcacggcccg ctcgctgcgg cgcaaggaat accgcattgg cggcaaaacc ggcacggccc
     2521 agaacccgca cggcaacgac cacgcctggt tctgcggcgt cgccccgctc gaggcccccg
     2581 agatcgtcgt cgtcgctatc gtcgagaacg ccggccacgg ttccacggcg gccgccccgg
     2641 tggtcggcaa gatcatcgac gcctacatga agaagaagcg cgatcccgag gccttcttcg
     2701 ccgaacagca ggcccgcgcg gacagcctgg ccgcggcgag agccctcgcg ggcctttccg
     2761 acacggcggc cgcgccggcg cgcgccgcga gggggaactg atgctcgact atcgccagct
     2821 ggactggaaa ctcatcggcg ccgtcttcgc gctctcggcc atcggggtgg ttctcatcat
     2881 gaccgcccag caccatgccg agagcgagta cgcccgcacc ttctacctgc gccagttgct
     2941 ctggctggtc atcgccctgg ccgcggccgc ggccgtgctc cacctgccct accggctgtt
     3001 cgacttcgcc gcctacattt tctacgccgt cgccgtgctc ctgctggtgg cggtgctgat
     3061 gttcggcgcc acgcggatgg gggcgacgcg ctggttttct ttcggcccca taaatttcgc
     3121 gccggcggat gtcgcaaaac tcgcactcat tttcgccctc gcccgcttct tcgcctactc
     3181 gaaaagcccc ctcctgtcgg gtcagcgggt ggcggtctcg ctcggactca ccgtcgtccc
     3241 catgatgctg atcctgaagc agcccgacct cggcaccgcc ctcactttcc ccgtggtcat
     3301 gtacgcactc tggttctggt cgggcattcc ccctctctac ctgctgctca tcgcctcgcc
     3361 cctgttgtcg ctggtagcgg ccagcaacgc gatctcgtgg tcgatctaca tggccgtgct
     3421 tctggccgtg ctcatcctgt tcaagccggg ccgccagctc atcttcagcg tcctcatcgt
     3481 cgtcgccaac ctcgccttcg gcgccgtcat gccgctcgtg tggaaccgtc tcgaggagta
     3541 ccagcaactg cgcatcatct cgttcgtcga tccctcgcag gatccgcgcg gcgccggcta
     3601 tcagatcatt cagtcgaaaa tcgccatcgg gtcgggcggt ctctggggca aagggtttct
     3661 cggcgggtcg cagaccaagc ttgatttcct ccccgagcgg cacaccgatt tcattttcag
     3721 cgtgctcggc gaggagttcg gcctctgggg ggcgctcatt gtcctcgccc tcttcttcgt
     3781 cttcttctac agggggattc gcatcgccgc ccgttcccgc tcgcgttttc tctcctatgt
     3841 ctctttcggg ggcgtggcga tcatcttctt ccagttcctc gtcaacatcg gcatgacgct
     3901 cggtctgatg cccgtcaccg gcgtgccgct cccgtttctc tcctatggcg gcacgtcgct
     3961 cgtcctctcg tgggtgctgg tcgcgttcct cctcaaagcc gactaccact ggcaggaata
     4021 ctgatccgcc ggggccgcgc tcacagctcc ccgctcagcc agtgcaggac gttcagtaga
     4081 aaccgggcat tctgcggcgc cgccgggctg ttcatcccgg ccggcgtccg gttcggaccc
     4141 gccagttgcg ccgagaacat cgccgcctcc ccgaacacgg ccacccgccc cgcgccgatc
     4201 actttcaccg ctccctgaca ccacccgctc accgcgaccg cggctgtgtt ggcgtcgaat
     4261 tgccaggcag tatcgggcgc aagcgacacc actccatcgc cgaacaccat cagcccggcc
     4321 gcgctatccg cacgaaaagc ctcgcccgta aacgacgcga ccgagtcgat ccgttcccta
     4381 tcgttgcgcc cgcgcgtgat cgcgtggtcg cgcagcgtgc cgtccgcccg gcggaagaca
     4441 tacgtgccgc cggtcggcga ggccgtgtcg acggcgaaac cgttgtgcaa atggaagccg
     4501 aaggccgcgg cgagttcggc cgccgccccg ggaaacggca tgtgatcggc gatcaacaag
     4561 agcgcgccgc ccccctgcac ccaggccgcg accgcggcga tttcgtcggg cgtgaacgcc
     4621 gagggcgtcg gcagcgacca ctcctgcgcg ttgcgctcat ggagcgcgtt ggagaccacc
     4681 aggatctccc cccgcgcgag cgaatccccc gtgaaccggc aacccgaggg gagcacgcgg
     4741 tacccgtcgc ggcgcagcag ctctgcgaac gtgtagtagc ggccgccggc ggtg
//
LOCUS       DAOOKS010000042         4732 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_21754_length_4732_cov_2.772290, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000042 DAOOKS010000000
VERSION     DAOOKS010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4732
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21754_length_4732_cov_2.772290"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(139..1215)
                     /locus_tag="PK112_01270"
     CDS             complement(139..1215)
                     /locus_tag="PK112_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HPC10710.1"
                     /translation="MFTLRELLEEMVKLNASDLHLTVGSPPVVRVDGRLQRLNYDVLT
                     SEQTKKLCYSMLTEKQKLKFEQNSELDFSFGIEQMSRFRCNVFMQRGNIAVVLRQIPY
                     KIRTFDELGLPKVIAEFAKLPRGLVLVTGPTGSGKSTTLAAIIDKINRERPVHIITVE
                     DPIEYLHRHQAALVNQREVYSDTTSFATALKYALREDPDVVLVGEMRDLETIEAALSI
                     SETGHLAFATLHTNSASESINRIVDSFPTNQQEQVRVSLSFSLQAIVSQALIPKIGGG
                     RVMALEIMVCTPAIRALVRDDKVHQMYSMIQSGQKYGMKTMNQSLSELYLTGKITLND
                     AMSYSSNTQELGEMLSRQKSPAFS"
     gene            complement(1361..3058)
                     /gene="pilB"
                     /locus_tag="PK112_01275"
     CDS             complement(1361..3058)
                     /gene="pilB"
                     /locus_tag="PK112_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013681489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HPC10711.1"
                     /translation="MPSDIGAMLVKAGRITPEQAEKADRMAREHGEKLEVALVRIGAV
                     ESEEEIAKFIGKHLKIGAIRLEDIELNPDIVKLIPVDIARKFKVIAVSKLNKTLLVAI
                     SDPNNIYVLDAVKFITGCIVQPVLSPERAIEKAIDQYYADGGGLSEIVKGLEDAEGLE
                     VVEAEDGPSDMDLMSAIQDKPLVKLVDSIIGDAIRMGASDIHIEMYEKRIRVRYRIDG
                     DLREMAPLPFKYRAAIVSRVKIMADLDISERRLPQDGRIKIKISGRTVDLRVSVLPTI
                     FGEKVVMRILDPNALMVDMTRLGFREEDLAKFDKAIHLPYGIVLVTGPTGSGKTTTLY
                     SALKQINTVDVNIMTAEDPVEFNFDGINQVAVKSDIGMTFAAALRSFLRQDPDIIMVG
                     EIRDGETAEIAIRAALTGHLVFSTLHTNDAPSSINRLIDMGVPYYLVASATKLILAQR
                     MMRKICQNCKEEINLSPEQVESLGAPKELLRNIRAFEGRGCRECGDTGSAGRTAVFEV
                     MPITPEIERLILNRAPDTDIAQKALEQGMFSLRMSAVEKMKAGLVSIDEVFAVTTNT"
     gene            complement(3144..3641)
                     /locus_tag="PK112_01280"
     CDS             complement(3144..3641)
                     /locus_tag="PK112_01280"
                     /inference="COORDINATES: protein motif:HMM:NF015232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HPC10712.1"
                     /translation="MSDDSLIIYEEEINQIDALISKMLKGAEAKCALLVDKDGHLITR
                     QGFTHSLDTTALAALLAGSFASTKEIAKLVGEPEFSVLFHQGKKDHIHMSLVGERSIL
                     VVIFDDRTTIGMVRLYAKETSAELAKIFDTIKATAAENKQQQAKFANEFAQGAQDKLD
                     DIFQD"
     gene            complement(4091..4666)
                     /locus_tag="PK112_01285"
                     /pseudo
     CDS             complement(4091..4666)
                     /locus_tag="PK112_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099276.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
BASE COUNT          767 a         1374 c         1609 g          982 t
ORIGIN      
        1 ggatgcggtt ctgccgcagg acccgttcga ggtcgccctt gctcttggct ttgagcgagc
       61 cttggacggc ggcgccggcg agcgtcttgc ctttatactc gaatactggc atggcaaacg
      121 tcctttccca tactcacatt acgaaaacgc cggagatttc tggcgggaga gcatttcgcc
      181 gagttcctgc gtgttcgagc tatagctcat cgcgtcattg agggttatct tgccggtcag
      241 gtagagctcg gagagcgact ggttcatcgt cttcatgccg tacttctgac ccgactggat
      301 catcgagtac atctggtgca ccttgtcgtc gcggacgagg gcgcggatcg cgggggtgca
      361 gaccatgatt tcgagcgcca tgacccgccc gccgccgatc ttcggtatga gcgcctgaga
      421 cacgatggcc tggagcgaaa acgagagcga cacccggacc tgctcctgct ggttggtcgg
      481 gaaggagtcg acgatgcggt tgatcgattc cgaggcggag ttggtgtgca gggtggcgaa
      541 ggcgaggtgg ccggtctccg aaatcgacaa agccgcctcg atcgtctcga ggtcgcgcat
      601 ctcgcccacg agcacgacgt cgggatcctc gcgcagcgcg tacttcagcg cggtcgcgaa
      661 cgaggtggtg tcggagtaga cctcgcgctg gttgaccagg gccgcctggt ggcggtgcag
      721 gtactcgatc gggtcttcca cggtgatgat gtggacgggc cgctcgcggt tgatcttgtc
      781 gatgatggcg gcgagagtgg tggatttccc ggagccggtc ggaccggtca cgaggacgag
      841 cccgcgcggc agcttggcga attccgcaat caccttgggc agaccgagct catcgaacgt
      901 gcggatcttg taggggatct gccggaggac gacagcgatg ttgccgcgct gcataaaaac
      961 gttgcagcga aagcggctca tctgctcgat gccgaaggag aaatcgagct cggagttctg
     1021 ttcgaatttg agcttctgct tctccgtgag catggagtag cacagcttct tggtctgctc
     1081 gctcgtcagc acatcgtagt tgaggcgctg cagacggccg tcgacgcgca cgaccggcgg
     1141 cgagccgacg gtgaggtgca ggtcggaggc gttgagtttc accatctctt ccagcagctc
     1201 gcggagtgta aacatctcga tctcccaatc tatgaggccg gcattcgttt tttcgggccg
     1261 atatcttctg tactattatc ggcgggcgag gcggaatctt gaccggaaag gcccgtggcg
     1321 ggctcgataa gcggttggcg gacaggcgga tttggagaga tcaggtgttc gtggtgaccg
     1381 cgaaaacttc gtcgatcgac accaggccgg ctttcatctt ctccacggcc gacatgcgca
     1441 ggctgaacat cccctgctcc agcgccttct gggctatgtc ggtgtcgggc gcgcggttaa
     1501 gaatcagccg ctcgatttcc ggggtgatcg gcatgacctc gaaaaccgcc gtccggccgg
     1561 ccgatccggt gtccccgcac tcgcggcacc cccgcccctc gaaggcgcgg atgttgcgga
     1621 ggagttcttt cggcgccccg aggctctcga cctgctcagg cgagaggttg atctcctcct
     1681 tgcagttctg gcagatcttg cgcatcatcc gctgggccag gatgagcttg gtggccgagg
     1741 cgaccaggta gtacgggaca cccatgtcga tgaggcggtt gatggacgac ggggcgtcgt
     1801 tggtgtggag cgtcgaaaac accaggtgcc cggtcagggc ggcgcggatc gcaatctcgg
     1861 ccgtctcgcc gtcgcgaatc tcccccacca tgatgatgtc ggggtcctgc cgcaggaagg
     1921 agcggagggc ggcggcgaag gtcatcccga tgtcggattt cacggccacc tggttgatac
     1981 cgtcgaagtt gaactcgacc gggtcctccg cggtcatgat gttgacgtcg acggtgttga
     2041 tctgcttgag ggccgagtac agggtggtcg tcttacccga cccggtcggg ccggtcacca
     2101 ggacgatgcc gtagggcagg tggatggcct tgtcgaactt ggccaggtcc tcctcgcgga
     2161 acccgagccg ggtcatgtcg accatgaggg cgttcggatc gagaatacgc atcacgacct
     2221 tctcgccgaa gatggtcggg aggacagaga cgcggaggtc gacggtgcgt ccggagatct
     2281 tgatcttgat gcggccgtcc tggggcaggc ggcgctcgga aatgtcgagg tcggccatga
     2341 tcttgacacg cgagacgatg gcggcgcggt acttgaaggg gagcggggcc atctcgcgca
     2401 ggtcgccgtc gatgcggtag cgcacgcgga tgcgcttctc gtacatttcg atgtggatgt
     2461 cggaggcgcc catgcggatg gcgtcgccga tgatcgagtc gacgagtttg acgagcggct
     2521 tgtcctgaat ggccgacatg aggtccatgt cgctcgggcc gtcttcggcc tccaccactt
     2581 cgagtccctc ggcatcctcg agccccttga cgatttcgga cagcccgccg ccgtcggcgt
     2641 agtactggtc gatcgccttc tcgatcgccc tctccgggga caggacgggc tggacgatgc
     2701 agccggtgat gaacttgacg gcgtcgagga cgtagatgtt gttcgggtcg ctgatggcga
     2761 cgaggagggt cttgttgagc ttggagacgg caatgacctt gaatttgcgc gcgatgtcga
     2821 cggggatgag cttgacgata tcggggttga gctcgatgtc ctcgaggcgg atggcgccga
     2881 tcttgaggtg tttgccgatg aacttggcga tctcctcttc cgactcgacg gcgccgatgc
     2941 ggacgagcgc gacttcgagc ttctcgccgt gttcgcgggc cattcgatcg gccttctcgg
     3001 cctgttccgg tgtgattctg ccggccttga ccagcattgc cccgatgtct gaaggcatag
     3061 ttcctcggtc agcacctcat gtgctgttta cccatgcagt cccgtgtcgt ccctccgccg
     3121 caccaccgca ggggcggagt ctctcagtcc tggaagatgt cgtccagctt gtcctgagct
     3181 ccctgggcaa actcgttggc gaatttcgcc tgttgctgtt tgttttccgc ggccgtggcc
     3241 ttgatggtgt cgaagatctt ggccagctcg gcggacgtct ccttggcgta gagccgcacc
     3301 atcccgatcg tcgtccggtc gtcgaaaatg acgacgagga tcgagcgctc gccgaccagg
     3361 gacatgtgga tgtggtcctt cttcccctgg tggaagagca cggagaactc gggttcgccg
     3421 acaagcttgg cgatttcctt agtggacgcg aacgagccgg ccagcagggc ggccagggcg
     3481 gtcgtgtcga gcgagtgggt gaagccttga cgggtaatca ggtgtccgtc cttgtcaacc
     3541 aggagggcgc atttcgcctc ggctcctttg agcatcttgg agatcagcgc gtcaatctgg
     3601 ttgatttctt cttcgtaaat aatgagacta tcgtcagaca ttcctgcctc ctgggtggct
     3661 atttcaatcc aaacagacgc ttaaagaagc cgcgcttctt gtcctctttc ttgcgccgca
     3721 ggggcggcgc catgtggggc gaaacggcgc cggtgccgct tccggtcgcg gccgagaccg
     3781 gcgtccgggc gaggctgccg gcggcggcga ccgacggcgc cgaaggccgg ggcggctcgg
     3841 ccgtggtgac cgccgcaggc gcactctcgg ccgccgcgcc cgtgcgcgaa ccggggtacg
     3901 gacggtactg cacagactcg gggtcctcgg cggcggccgg gcggccctgg cgggcgaatt
     3961 ccgcggccgt cgcgggcggc gtctcggtcg cggccgcctc ctcgcgatcg tccacccgcg
     4021 gagcctcggg cgccggagca acccggtcct ccatcggcgt gatcgcccgc ggccgcgaac
     4081 tgcccgaact cttggccttc tccagcacca ttttgataat catcttcagg gtgtcgaaca
     4141 cgccgttgcc gacggtcgcc gaggcctcga acgacggcca gccgcgcggg ttgagctggc
     4201 gctcgagttc agcgacgggg aggacgccgg ggaggtcgcg tttgttgtac tggatgacga
     4261 ccggcagctc gccgatgtcg tagccgtact cgcggaggtt ctcctcgagg ttggtcaggg
     4321 attcaatatt ctcgtccatc ttgctcggct ggctgtcggc gacgaagacc agaccgtcga
     4381 cgccgcgcag cacgagcttg cgcgtcgcgt tgtaatagac ctggcccggg acggtgtaga
     4441 gctgaaactt ggcggcgaag ccgttgatgg tgccgatgtt gatcggcagg aagtcgaagt
     4501 agagggtgcg gtcggcttcg gtggccagcg aaatcagctt gccgcgggtg ttgcccggca
     4561 ccttggcgtg gacgtactgg aggttggtcg tcttgcccga caaccccggt ccatagtaga
     4621 caatcttaca gcacacttcg cgggcggagt aattaatcga taccatgtcg tctcgtccct
     4681 atgtcgtcgt tggtcggcat gcggttaccg gacggtgcgc aggcgttcga tc
//
LOCUS       DAOOKS010000043         4708 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_21892_length_4708_cov_3.026649, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000043 DAOOKS010000000
VERSION     DAOOKS010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4708
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21892_length_4708_cov_3.026649"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="PK112_01290"
     CDS             <1..615
                     /locus_tag="PK112_01290"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="catalyzes the formation of
                     5-O-(1-carboxyvinyl)-3-phosphoshikimate from
                     phosphoenolpyruvate and 3-phosphoshikimate in tryptophan
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HPC10713.1"
                     /translation="RGGTVDIPGDISTAAFFFAAAAISGKTITVERVGLNPTRTGILE
                     HLRGLGCRVDISERETVTGEPRGTVAVTGAPLGARRISGETTVELVDEIPVIAVMAAF
                     AEGTTVIRDAGELRVKESDRLAAVADNLKRMGAKCGLLEDGLAIEGGGQLEGADFRTY
                     GDHRIAMAFAVASLFLTGPSTLDDDECVGVSCPTFFDLLNQIRA"
     gene            612..1457
                     /locus_tag="PK112_01295"
     CDS             612..1457
                     /locus_tag="PK112_01295"
                     /inference="COORDINATES: protein motif:HMM:NF020090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HPC10714.1"
                     /translation="MTGRLSFALIGEHIAYSRSPAVFEAIFRADGVEGSFRVHEVSAA
                     AFAEEFPRLLRSGVHGLAVTIPHKERVAPFLDDLDPVARALGAVNSIAIADRLRGFNT
                     DVAGFRFALENYRAVVAGRPALVLGCGGAARAAVYALHRHFGVPSIVITSRRAETLER
                     TVERLRKGMAGLEITPAPAARLLPLDRPVGVVVNATPLGGGNHPRELPLPKAFDWTRA
                     AVYYDLNYNDDNQAVGCAREAGVAALDGAAMLVAQAIESYRRWTGRTVPFDPVFAAAF
                     PRRTR"
     gene            1476..2045
                     /locus_tag="PK112_01300"
     CDS             1476..2045
                     /locus_tag="PK112_01300"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HPC10715.1"
                     /translation="MKKAKDPEKIFLVGFSGSGKSTIGPLLAARLGRFYVDTDRVIAE
                     KFGKDIPGIFADDGEEAFRAAETEVLGEIAGRAGVREVVVLGGGAFTIAVNRDLVRRA
                     GIAVYIQCSVRELARRMRRHADRPLLTVVPNPGESLPEATRRRVAELLEERREFYLLA
                     DMTVSSTARLPVSVVEEIVRKLENRYEPS"
     gene            2032..3168
                     /gene="aroB"
                     /locus_tag="PK112_01305"
     CDS             2032..3168
                     /gene="aroB"
                     /locus_tag="PK112_01305"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HPC10716.1"
                     /translation="MSRVDVCLGDRRYPVLIGEGGRERDLPRLVHRQARRGGRVFVIF
                     DAQVCALYGPELVARLADSGGSIHQMVLPSGERFKTLSVAAAVYDFLLRRRVARDDFL
                     LAVGGGVTTDLAGYTAATILRGIPWGAVPTTLLGMVDAAIGGKTGVNHPLGKNLIGAF
                     WQPRFVLCDVGFLPTLPDRELVAGLGEVVKYAGLAGETFLRLLESYLDGGNLLEARSL
                     SRLVRLSVAYKAGVVVRDERESGPRMALNLGHTFGHAVEQAAGFGRLRHGEAVLLGLD
                     AAVELSIAQKPSRRAGLAGYRALVRRLLGVLPRRRISAEAVMEALALDKKRAGTTARF
                     VLLDAPGRPLIGTEVPAAAVRDAVRAMLEHYAPEGGRHVQSPRR"
     gene            3146..3583
                     /gene="aroQ"
                     /locus_tag="PK112_01310"
     CDS             3146..3583
                     /gene="aroQ"
                     /locus_tag="PK112_01310"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003183415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HPC10717.1"
                     /translation="MSRVLVVNGPNLNLLGRREPEIYGTATLEDLHTRLREFAAEMKM
                     ELAFFQSNAEGEIIEFIQREGPGADGLVINPGALAHYSHALRDAITAVGIETVEVHLT
                     NIYAREEFRRHSVIAPAARGQVVGFGMYGYAMALSYFADTSDK"
     gene            3606..>4708
                     /locus_tag="PK112_01315"
     CDS             3606..>4708
                     /locus_tag="PK112_01315"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HPC10718.1"
                     /translation="MKRLVLLLIGLSLLAAACREQPVSRDDVFLVMDSLEHKFDWLGY
                     RLQQETWEIYSTGQSDSLDFYQGLLRYTVADPNLLRTFRQGRLWVTENEDVRRLDLLE
                     AALMRAQVEVAEPVASLHDSLVQIDVTFRAPFDGQNRTQNYLYRTYRSDPDRSRREAA
                     FRAYHRIGDLLADGLARLIRLRNREAAKFGQQNYFSLLFSQQGFDEARYLATLDQLDS
                     LSLEPYRRILANAGEKLGSGPVEIWDLSYAYADINRRGDAYFPADRQFSQIARSLEAL
                     GYDLDKLPIYFDLQPRSDKTQLAYAVPLRPPHDVRVLANVTDGYLSARTLMHEIGHAL
                     HFAHIRQERQLFVDCVSEAWTEGMAQIFASLLD"
BASE COUNT          715 a         1605 c         1568 g          820 t
ORIGIN      
        1 cgcggcggca cagtcgacat tcccggcgac atctcgaccg ccgccttctt tttcgccgcc
       61 gccgccatat ccggcaagac catcaccgtc gagcgcgtcg gccttaaccc gacccgcacc
      121 gggatcctcg agcacctccg gggccttggc tgccgggtcg acatcagcga gcgcgaaacg
      181 gtcacgggtg agccgcgcgg cacggttgcc gtcaccggcg ccccgctcgg ggcgcgccgc
      241 atctccggcg aaaccaccgt cgaactggtc gacgaaatcc cagtcatcgc cgtcatggcc
      301 gcatttgcgg aggggacgac cgtgatccgc gacgccggcg agctgcgggt caaggagagt
      361 gaccgcctgg ctgcggtcgc cgacaacctc aagcgcatgg gcgccaaatg cggactactc
      421 gaagacggcc tggcgatcga gggcggcgga cagctcgaag gggccgactt ccgcacctac
      481 ggcgaccacc gtattgccat ggccttcgcc gtcgcctcgc tcttcctcac cggcccgtcc
      541 acgctcgacg acgatgaatg cgtcggcgtc tcctgcccga cgttcttcga cctcctcaac
      601 cagatacggg catgaccggc cggctctcgt ttgcgctgat cggggagcac atcgcgtact
      661 cccgttcgcc ggccgtattc gaggccatct tccgggccga tggagtcgaa ggctcgttcc
      721 gggtgcacga ggtctccgcc gcggcgttcg ccgaggagtt cccgcggctc ctgcggtccg
      781 gggtgcacgg tctggccgtc accatcccgc acaaggagcg ggtcgcgcct ttccttgacg
      841 acctcgatcc ggttgctcgg gcccttggcg ccgtcaattc catcgccatc gcggaccgtc
      901 tgcgcggttt caacaccgat gtcgccggtt tcagatttgc cttggagaat taccgggcgg
      961 tggttgcggg acgaccggcc ctcgtgctcg gctgcggggg ggcggcccgg gcggcggtgt
     1021 acgccctcca ccgccacttc ggggtaccgt ccatcgtgat cacctcgcgc cgcgcggaga
     1081 cgctggagag gacggttgag cggctccgca agggcatggc gggcctggaa atcacgccgg
     1141 cgccggcggc gcgcctgctg ccgctcgacc gtcccgtggg agtagtggtc aacgccactc
     1201 ctctgggcgg cggaaaccat ccccgcgaac tgccgctgcc gaaagcgttt gactggacgc
     1261 gcgcggcggt gtactatgac ctcaactata acgatgacaa tcaggcggtc ggctgcgcgc
     1321 gcgaggccgg cgtggcggct ctcgatggcg ccgcgatgct ggtcgcccag gcaattgaat
     1381 cataccggcg ctggaccggc cgaacggtcc cgttcgatcc ggtgttcgcc gccgccttcc
     1441 cgcggcggac gcgctgaatt cgagccccga gagcgatgaa gaaggcgaag gaccccgaga
     1501 agatttttct ggtcggtttt tccggaagcg ggaaatcgac aatcggcccg cttttggccg
     1561 cgcgcctcgg gcgtttctat gtcgataccg accgggtgat cgcggagaag ttcggcaagg
     1621 acatccccgg gatattcgcc gacgacgggg aggaggcgtt ccgggccgcc gagaccgagg
     1681 ttctcggcga gatcgcgggc cgcgcgggcg tgcgcgaggt ggtcgtgctg ggcgggggcg
     1741 ctttcaccat cgccgtcaac cgcgacctgg ttcgccgcgc cggaatcgcc gtctacatcc
     1801 agtgttccgt tcgcgagctc gcccgccgca tgcgccgcca cgccgaccgc ccgctgctga
     1861 cggtcgtccc caaccccggc gaatccctcc ccgaggcgac ccgccgacgg gtggccgaac
     1921 tgctcgagga gcgccgcgag ttctaccttt tggccgacat gaccgtgtcg tccaccgccc
     1981 gcctgccggt cagcgtggtc gaagagatcg tgcgcaagct ggagaaccgc tatgagccga
     2041 gttgatgtct gtttggggga tcgccgctac ccggtgctca tcggggaagg cggccgggag
     2101 cgcgatttgc cgcgccttgt gcaccgccag gcgcggcggg gcgggcgggt ctttgtcatt
     2161 ttcgatgccc aggtctgcgc tctctacggc ccggaacttg ttgcacgtct cgccgacagc
     2221 ggcggttcga tccaccagat ggtgcttccc tccggcgaac gtttcaagac tctctcggtc
     2281 gcggccgccg tttacgactt cctcctgcgc cgtcgggtcg cccgcgacga tttcctgctg
     2341 gcggtcggcg gcggagtcac gaccgacctg gcgggttaca cggccgccac tatcctgcgg
     2401 gggatcccgt ggggagcggt cccgacgacg ctcctgggca tggtcgacgc cgccatcggc
     2461 ggcaagaccg gcgtcaatca cccgctggga aagaatctga tcggcgcgtt ctggcagccc
     2521 cggttcgtgc tctgcgacgt gggatttctg cccaccttgc ccgaccgcga actggtcgcc
     2581 ggtctcggcg aggttgtaaa gtacgcgggg ttggcggggg agacgttcct gcgcctcctg
     2641 gagtcgtatc tggatggcgg gaatcttctg gaggcgcgct ccctcagtcg cctggtgcgt
     2701 ctgtcggtgg cgtacaaggc cggcgtggtc gtccgcgacg aacgtgagtc cggcccccgc
     2761 atggcgctga atcttggaca cacattcggc cacgccgtgg aacaggcggc cgggttcggg
     2821 cggctgcgcc atggcgaggc cgtgctgctg gggctcgatg ccgcagtcga gctgagcatc
     2881 gcgcagaaac cgtcgcgccg ggccgggctc gccggctacc gggcgctggt tcgccgcctg
     2941 ctcggggtgc ttccgcgtcg ccgcatatcg gccgaggcgg tcatggaagc gctcgcgctg
     3001 gacaagaaac gcgccggaac gacggcccga tttgtcttgc tcgacgcccc gggccggccg
     3061 ttaattggga ccgaggtccc ggccgcggcg gtgcgcgacg cggtgagggc aatgctcgag
     3121 cactatgcgc cggaaggagg ccgccatgtc cagagtcctc gtcgttaacg ggcccaatct
     3181 caatctgctc ggtcggcgcg aaccggagat ttacggcacc gccaccctgg aggatctcca
     3241 cacccggctc cgcgaattcg ccgcggagat gaagatggag ctggcgttct tccagtccaa
     3301 cgccgagggc gaaatcatcg agttcatcca gcgggagggc ccgggcgccg acggcctggt
     3361 aatcaacccg ggcgccctgg cgcactacag ccacgctctg cgcgacgcta tcacggccgt
     3421 gggaatcgag accgtcgagg tccatctcac gaacatctac gcccgcgagg aatttcggcg
     3481 ccactccgtg atcgcgccgg ccgcgcgcgg ccaggtggtc ggattcggca tgtacgggta
     3541 cgctatggcc ttgtcgtatt ttgccgatac ttcggataaa tgaccgatcc ccggattggc
     3601 gcaccatgaa acggcttgtg ttactgctca taggcctcag cctgcttgcg gcggcctgcc
     3661 gggaacagcc tgtctcgcga gacgacgtgt tcttggtgat ggactccctc gagcacaagt
     3721 tcgactggct cggctaccgc ctccagcagg agacctggga gatctacagc acggggcagt
     3781 ccgactccct ggatttctac cagggactcc tccgctacac ggtcgccgat ccgaatctgc
     3841 tgcgcacctt ccgccagggc cgcctgtggg tgacggagaa cgaggacgtc cggcgcctcg
     3901 acttgctgga agcggcgctc atgcgggccc aggtcgaggt ggccgagccg gtggcctccc
     3961 tccacgactc gctcgtgcag atcgatgtca ctttccgagc gcccttcgac ggccagaacc
     4021 ggactcaaaa ttacctgtac cgtacgtatc gatccgaccc cgaccgcagc cgccgcgagg
     4081 cggcctttcg cgcctaccac cgcatcggcg atctgctggc cgacgggctg gcccggctga
     4141 tccggctgcg caaccgcgag gcggccaagt tcggacagca gaactatttc agccttctct
     4201 tttcgcagca ggggttcgac gaggcccgct accttgccac cctggaccag cttgactcgc
     4261 tgagtctcga gccctaccgc cggatactgg cgaacgcggg ggagaagctg gggtccgggc
     4321 cggtggagat ctgggacctc tcctacgcgt atgccgatat caaccggcgg ggcgacgcct
     4381 actttccggc cgaccggcag ttcagccaga ttgcgcgcag cctggaggcg ctgggctacg
     4441 acctcgacaa actgccgatc tatttcgacc ttcagccgcg cagcgacaag acgcagctcg
     4501 cctacgccgt cccgctccgg ccgccccacg acgtccgcgt gctggcgaat gtgaccgacg
     4561 gctacctgag cgcgcgcacg ctcatgcacg aaatcgggca tgcccttcac tttgcccaca
     4621 tccggcagga gcggcagctt tttgtcgact gcgtgagcga ggcctggact gaggggatgg
     4681 cgcagatttt cgcctcgctg ctcgacga
//
LOCUS       DAOOKS010000044         4699 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_21955_length_4699_cov_3.584410, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000044 DAOOKS010000000
VERSION     DAOOKS010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4699
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21955_length_4699_cov_3.584410"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1897
                     /locus_tag="PK112_01320"
     CDS             <1..1897
                     /locus_tag="PK112_01320"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HPC10719.1"
                     /translation="DTLDYSGSARISDMLLEYRQVPGQLRVPSATVDFKRDNARLNIS
                     DASFAGKPLKGYVYVENFAAPVVNAELTGEINLGLLRPFLPAANRPELVGDASFSVKA
                     SGPVRQVEELEVSGNVRVANGRYAAAMLPEPVESFSLDVYFDKSVVRVNALDARMPSG
                     NLSFKGRVNDLLPYLMADSLHPVKTGPAVDGTLEAQVNLAMLNPFLPKQGKPEIAGKL
                     QTSLQLAGDILEPSGLRVRGTLAVGEGAYRDTALAEPIEHFDTRMTIIPDTIRIDELN
                     IRFTTSDLSLTGSLIDPFPYLLPLKSVDRTRAAKPFLQFAMTSHRFNVDSLFPDTLPS
                     GDAADGGPAAGDTLVPFILPDIDGRGVARFDTVIYWNVPFSDLTGDVTIKDRVVRVTN
                     VVGRVYTGTVTGETSIDLNNWLAPAYDGRFNATDIEANDFARRFTRFGDFVYGKFNLD
                     GSFQASGWDADAIMYSLNMNSAARMREGRLETSGAVLTALQTVGSLVGEQLDRTFNLR
                     NARTDIAVDSGRVRFDKLSAALGDMGDLELNGFYSFAGEVNYTGTLLLSPELTQKFLS
                     QGVLGEVSSFLGGSSAVKQVKLPVTIGGSLDDPKVDINLKSITEQAGKSAVEGAADKL
                     KGLIKKP"
     gene            2145..4007
                     /locus_tag="PK112_01325"
     CDS             2145..4007
                     /locus_tag="PK112_01325"
                     /inference="COORDINATES: protein motif:HMM:NF013179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HPC10720.1"
                     /translation="MPTVRQQKPGAAVEMNARAIARCGVCKVDLKGRFVFVDEQAQQL
                     FGCPGEELFGKALIDFVDEASRAVVNEILSRRNHYEITFDHIGLTLVVPGAAAIRAGA
                     IVTLNFNAGNPVNFQFIFLADNAVAVDLVRDPHAAAYRRLVAYLDGRDLTADWRGYLG
                     ELQSFSGAVQVCAYLVSPDRLEPRSSALDAGDGVTSLPSIDPTGPLHRQVADTGETYD
                     FTDQRSIQRAVELGAPAPNEVIARLALDEGQAALIRVIFPSDIADNDARVQADRTRFA
                     LELTQRLLAPPPPSADDRTADNIQFAIGLLDGLGIGAMMADSRGKTAVYNPVLARLFQ
                     VEDVSVDLVELAEILSGGAAEERQRLDDFLRGAARPSTGALELARVELPAGRPGLLAV
                     LPLAENPNDRTAFVAIVPGALAADAADVLAFGRTLLSRLGADTAEAVALSARLGQRSA
                     ALDAEGKDQLHRLTESLQDLGRLTQDLRSALDISSRVQAPELTDLNIVLHRVVNHVNR
                     GRGGQPLRVHTDLMPKVSTSRRAVTLALENLLQAVATGADRATATVRIAAELSGPSAC
                     RLTITVDSPLDRSHNDFAFGFSAARLLIRSIGGTLREEPRTAGVGFVIAFPVQP"
     gene            4179..>4699
                     /locus_tag="PK112_01330"
     CDS             4179..>4699
                     /locus_tag="PK112_01330"
                     /inference="COORDINATES: protein motif:HMM:NF015699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10721.1"
                     /translation="MDSMTGFGKAEFKSALGTFTVEVSSVNNRFLEVSARLPRQFSAM
                     EPKVRELVGRHVNRGKVYIYVGFSEGEENTDRFSINVKAAAAYARQLAALRDQLHIGG
                     EVTIGDLLVFPEVVKSDETAFEDEEIWEQLEAPLAEAMTALVRMRRREGEAMARDMKQ
                     RLDQIKEITRAVE"
BASE COUNT          796 a         1655 c         1427 g          821 t
ORIGIN      
        1 ggacacgctc gattactccg gttccgcccg catcagcgac atgctcctcg aataccggca
       61 ggtgcccggc caactgcgtg tcccgtccgc caccgtcgac ttcaagcgcg acaacgcccg
      121 cctcaacatc agcgacgcat cgttcgccgg aaagccgctc aaggggtatg tctacgtcga
      181 aaacttcgcc gcccccgtcg tgaacgccga actgaccggg gaaatcaacc tcggcctgct
      241 ccggccgttt ctcccggctg cgaatcgccc cgagctggtc ggcgacgcgt cgttttcggt
      301 caaggcgtcc gggccggtcc gtcaggtgga ggagctggag gtatcgggaa atgtccgggt
      361 ggccaacggt cgctacgccg ccgccatgct ccccgagccg gttgaatcgt tttcgctcga
      421 cgtgtacttt gacaaaagcg tggtccgcgt caacgccctc gatgcgcgca tgccctcggg
      481 caatctctcg ttcaaagggc gggtcaacga tcttctccct tacctcatgg ccgattccct
      541 tcacccggtg aaaaccgggc cggcggtcga cggcacgctc gaggcgcagg tgaatctcgc
      601 catgcttaac cccttcctgc cgaaacaggg aaaacccgag attgcgggca agctccagac
      661 cagcctccag ttggccggtg acatcctcga accctccggc ctgcgcgtgc gggggacgct
      721 ggccgtgggc gagggggcct accgcgatac cgccctggcc gaaccgatcg aacacttcga
      781 cacgaggatg acgatcattc ccgatacgat caggatcgac gaactcaaca tcaggttcac
      841 cacttccgac ctgagtctca ccggatcgct gatcgatccc ttcccgtatc tgctgccgct
      901 gaagtcggtg gatcgaacgc gggcggccaa acccttcctc cagttcgcca tgacctcgca
      961 ccgcttcaat gtcgacagcc tctttcccga caccctcccc tccggcgacg ccgccgacgg
     1021 cggtccggcc gccggcgata ccctcgtccc cttcattctc cccgacatcg acggccgggg
     1081 ggtcgcgcgc ttcgataccg tcatctactg gaacgtgccg ttctccgatc tcaccggcga
     1141 cgtcacgatc aaagatcggg tggtccgcgt caccaatgtc gtcggccgcg tctacaccgg
     1201 cacggtgacg ggggagacct ccatcgacct caacaactgg ctcgccccgg cctacgacgg
     1261 ccggttcaac gccaccgaca tcgaggctaa tgatttcgcc cgccgcttca ccaggttcgg
     1321 cgatttcgtg tacggcaagt tcaacctcga cggcagcttt caggcctccg ggtgggatgc
     1381 cgacgccatc atgtactcgc tgaacatgaa cagcgcggcg cgcatgcgcg aggggcggct
     1441 ggaaacctcg ggcgccgttc tcaccgcgct gcagaccgtc gggagtctcg tcggcgaaca
     1501 gcttgaccgc accttcaacc tccgcaatgc ccggaccgac atcgccgtcg acagcggccg
     1561 cgtccgcttc gataagctct ccgccgccct cggcgacatg ggcgatctcg aactgaacgg
     1621 attctattcg ttcgccggcg aggtcaacta caccgggacg ctgctgctca gtcccgaact
     1681 cacgcagaaa ttcctctccc aaggcgtgct cggcgaggtc tcgagtttcc tgggcggcag
     1741 ttccgctgtg aaacaggtga agctgccggt caccatcggc ggttctctgg acgacccgaa
     1801 ggttgatatt aacttgaaat cgatcaccga acaggcgggc aagagcgcgg tcgagggcgc
     1861 cgccgacaag ctcaagggcc tgatcaagaa accgtaggcc gcggccggcc cgcgcccgcc
     1921 ggaccgtcgc gtcgcggccg tccgcaaccg atgcccctct gacggggcag aaccctcgcc
     1981 gatctgcggg gcaattctgc cccgcaaagt tgtcccccgc cggtgaataa tcccctgcat
     2041 gtgtccgata taaccgaagt acgggacgac ggcgggcggt ccgccggggg gagatgcctt
     2101 cggctgccgc cggcaagcgg cgggcgatgt tggaagaggt ttgcatgccg acggttcgac
     2161 agcagaaacc gggcgccgcg gtggagatga acgcccgcgc gatcgcccgc tgcggcgtgt
     2221 gcaaggtcga tctcaagggg cgctttgtct ttgtcgatga gcaggctcag cagctcttcg
     2281 gctgcccggg tgaagagctg ttcggaaaag ccctcatcga ctttgtcgac gaagcctcgc
     2341 gggccgtggt caacgagatt ctctcccgtc gcaaccacta cgaaatcacg ttcgaccaca
     2401 ttgggttgac gctcgtggtt cccggcgccg ccgccatccg ggccggggcg atcgtcacgc
     2461 tcaatttcaa cgccggcaac cccgtcaatt tccagttcat ctttctggcc gataacgccg
     2521 ttgccgtcga cctcgtgcgc gacccgcacg cggccgccta ccgacggctg gtggcctatc
     2581 tcgacggccg cgatctcacc gccgactggc gcggctatct cggcgaactc cagtcgttct
     2641 ccggggccgt gcaggtgtgc gcctacttgg tctcgcccga ccggctcgag ccgcgcagca
     2701 gcgcgctgga cgcgggcgac ggcgtcacct cgcttccctc gatcgacccg accggccctc
     2761 tccaccgcca ggtcgccgac acgggggaga cctacgattt caccgaccag cggtcgatcc
     2821 agcgcgccgt tgaacttggc gcacccgctc cgaacgaggt gatcgcccgc ctggctctcg
     2881 acgagggtca ggcggctctc atccgggtga tctttccttc cgacatcgcc gacaacgatg
     2941 cccgcgtaca ggccgaccgc acccgcttcg ccctcgagct cacccagcgg ctgctggccc
     3001 cgccgccccc ctctgccgat gaccgcacgg ccgacaacat ccagtttgcg atcgggctgc
     3061 tcgacggact cgggatcggc gccatgatgg cggactcgcg cggcaagacc gccgtctaca
     3121 atccggtgct cgcccggctt ttccaggtgg aggatgtctc cgtcgatctg gtggaactgg
     3181 ccgagattct ctccggcggc gccgccgagg agcggcagcg cctcgatgat ttcctgcgcg
     3241 gcgcggcgcg cccgagcacc ggcgcgctgg agctggcgcg cgtcgaactc cccgccgggc
     3301 gtcccggcct gctggccgtt ctcccgctgg ccgagaatcc caacgaccgc accgcctttg
     3361 tcgctattgt ccccggcgct ctggccgccg acgccgccga cgtgctcgcg ttcgggcgga
     3421 ccctcctgag ccgtctcgga gccgacaccg ccgaagccgt ggcgctgagc gcccggctgg
     3481 gtcagaggag cgcggctctg gatgcggagg gcaaagacca gctccaccgg ctcaccgagt
     3541 cgcttcagga tctcggccgc ctgacgcagg atctccggtc ggcgctggac atctccagcc
     3601 gggttcaggc gcccgaactg accgacctca acatcgtcct ccaccgcgtc gttaaccacg
     3661 tcaaccgcgg ccggggcggc cagccgctgc gcgtccacac tgacctgatg cccaaggtct
     3721 caacgtcccg gcgcgccgtg acgctggcgc tggagaacct gctccaggct gtcgccaccg
     3781 gtgccgaccg ggcgaccgcc accgtgcgga tcgccgccga acttagcgga ccctcggcct
     3841 gccgcctgac aatcaccgtc gactcccccc tcgatcgaag ccacaacgac ttcgcgttcg
     3901 gtttcagcgc ggcccggctg ctcatccgca gcatcggggg cacgctgcgc gaggagcccc
     3961 gcaccgccgg cgtcggcttc gtgatcgcct tcccggtgca gccctgaacg atcgttcgcg
     4021 gcgccgccaa ccctgcgccg ttggccgcct tgctccttcg tggacaagct cttagcagca
     4081 gccggacgag aaaagtgaca cgccgctccc ggttttgcgg ttgacaaaag attcccaccc
     4141 cctattttgg accctgtctt tcggcaggaa ttatgacaat ggattcaatg accggtttcg
     4201 gcaaggccga attcaagtct gcactgggta cgttcaccgt ggaggtttcc agcgtgaaca
     4261 accgtttcct ggaggtatcc gcccgattgc cgaggcagtt ttcggcgatg gagccaaaag
     4321 tgcgcgagtt ggtcggccgc catgtgaatc gcggcaaagt ctacatctat gtcggattct
     4381 ccgaagggga ggagaatacc gatcgattct ctattaatgt gaaggccgcc gcggcctatg
     4441 ctcgccaact ggccgccctg cgcgaccaac tccatatcgg gggtgaggtt acgatcggcg
     4501 acctgctcgt gttccccgag gttgtcaaaa gcgacgaaac cgctttcgag gacgaggaaa
     4561 tctgggagca gctcgaggcc ccgctggctg aggccatgac cgctctcgtg cgcatgcggc
     4621 gccgcgaggg agaagctatg gcgcgcgata tgaagcagcg tctcgaccag atcaaggaga
     4681 tcacccgggc ggtcgagaa
//
LOCUS       DAOOKS010000045         4685 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_22035_length_4685_cov_3.536717, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000045 DAOOKS010000000
VERSION     DAOOKS010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4685
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22035_length_4685_cov_3.536717"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            29..1330
                     /locus_tag="PK112_01335"
     CDS             29..1330
                     /locus_tag="PK112_01335"
                     /inference="COORDINATES: protein motif:HMM:NF016354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HPC10722.1"
                     /translation="MKILYVDPGSPLFGYVRPGYRIVSVNGREVIDTIDLRYKLAEER
                     VTIVFADPAGRELSFDFAEDEGAGESLAAGLGLTLDDSRIRTCKCDCIFCFVRQQPTG
                     MRRALYTKDEDYRLSFTHGNFITLSNMTEADLARIIEQRLSPLYISVHAADDTLRRCM
                     LRNEKLAPIVPTLRRLGGHGITMHTQVVLCPGINDGEALAQTIDELAALFPQVATLAV
                     VPVGLTRFREGQTKLRTYTREEAGGIIDYIERRQKVFRRERGSRFVWPADEFYVLGGR
                     TFPNRAEYEDMAQFENGIGMVRETITGFNRRRAMLRKVRGRQRVLFLTGESARPFLES
                     HVAAYARDQLGLRLEVEAVKNEFWGPTVTVSGLLTGQDLLRHARRRHENWDVLVVPPN
                     CLNDDDLFLDNLSLEQFRGVLAKPVLVGQYNVAASLKEAFS"
     gene            1327..2640
                     /gene="der"
                     /locus_tag="PK112_01340"
     CDS             1327..2640
                     /gene="der"
                     /locus_tag="PK112_01340"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HPC10723.1"
                     /translation="MKLPTVAIVGRPNVGKSSLFNRFLRKYLAVVDETPGVTRDRNYA
                     VCDWAGREFRLIDTGGLVPKSGDLMNRLIYEQADFAIHEADLVLLVVDAMVGADYIDE
                     EIARRLQQSAKPTILVANKADNPRLELEVYDFLRLGLGEPMAVSATVGRGIGELLDKI
                     VALLPEAPADDVGGSGAVRVAVVGRPNVGKSSFINKLTGEERAIVTPIAGTTRDAVDT
                     PFSFEGRNYILVDTAGLRRKFKVHEDIEFYTTLRTSRAIDSAEVAVVLIDAQDGVTSQ
                     DQRILEDVVETRCAAVLAVNKWDLIEKETGTAERFARQINEALAHYSYLPLVFISALT
                     GQRVGKVLALVDQVHAENTRQLPTPELNDWLAEVTARRHPPARQGKHIKFNYITQTGA
                     APPSFVIFANYPKLVDRSYVNYLHNQLRERWGFAGVSLRLKFRRK"
     gene            2726..4063
                     /locus_tag="PK112_01345"
     CDS             2726..4063
                     /locus_tag="PK112_01345"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPC10724.1"
                     /translation="METRPVYDVDPEQLAASLAARIADLQDLATMGAVIASIHDIDAI
                     LSVVMDMSLRLVDGEVGAILLEKSGQLGIEISWGVQEGLIPKLPGGETAEELLPAGEA
                     APPGGMDLPTRVLASGQTVILNDLSLRDEDGRRIDAVICMPIGTRDRRYGVLLILNRA
                     GGGGFNDEDRERLAMLLNFVAVAIDNARLMQEQLSRQRIEQEMAIARQVQETILPQDI
                     DAVGGVEIGASYYPAQEVGGDFYDVHALSDNEFLVFIGDVSNKGVPAALVMSAAAGII
                     KSILDRSPEIAVNELASRLNEILAREIIRDREMFITLFFARFDLARGRMTYCNGGHVP
                     GMFWSAREGTVARLSTGGPIVGQFPGAHFDLGSCPIGSGDRLFLCTDGLTEAVDKDGR
                     IFGREQAEEVFAMEIGLPPKEFCVRIREWIDRFASGAPEDSHDDFTVLQVLVK"
     gene            4063..4485
                     /locus_tag="PK112_01350"
     CDS             4063..4485
                     /locus_tag="PK112_01350"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPC10725.1"
                     /translation="MATHRFAYPSVVESEERFLNDLAQVAQRERLEEDVRRAMMLTLS
                     EAFTNALVHGNRLDPAKTVWVEVRVDEATVSAEVADEGNCGLAELAARPQTGQLDDHG
                     RGIGLMEHFAAEVVFGETLTGGLKVSVRFRRLVERTRG"
BASE COUNT          844 a         1488 c         1554 g          799 t
ORIGIN      
        1 gaggtgatgt ctgcaaggtg aatgtgcgat gaagatactc tatgtcgatc cgggatcccc
       61 cctgttcggc tacgtccggc cgggataccg catcgtgtcg gtcaacggcc gggaagtgat
      121 cgacaccatc gaccttcggt acaaactggc agaggagcgg gtgacgatcg tcttcgccga
      181 cccggcaggc cgggagctgt cgttcgattt cgccgaggac gagggggcgg gggagagcct
      241 ggcggccggg ctgggactga cgctcgacga ctcccggatc agaacctgca agtgcgactg
      301 catcttctgc ttcgtgcgcc agcagccgac cggcatgcgg cgggcgctct acaccaaaga
      361 cgaggactac cggctgtcgt tcacccacgg aaatttcatt accctctcca acatgaccga
      421 ggccgacctc gcacggatca tcgagcagcg cctgtccccc ctgtacatct cggtgcatgc
      481 ggccgacgat accctgcgcc ggtgcatgct ccgcaacgag aaactcgccc ccatcgtccc
      541 gaccctccgc cgcctcggcg gccacggaat caccatgcac acccaggtcg ttctgtgccc
      601 ggggatcaac gatggagaag cgctcgcgca aacgatcgat gaactggccg ccctctttcc
      661 gcaggtggcg acgcttgcgg ttgtcccggt ggggctcact cggttccgcg aggggcagac
      721 gaagctgcgc acgtacacgc gggaggaggc gggcgggatc atcgattata tcgagaggcg
      781 ccagaaagtg ttccggcgcg aacgcgggag ccggtttgtg tggccggccg acgaattcta
      841 tgtgctcggc ggccggacgt tcccgaaccg ggcggaatac gaggacatgg cccagttcga
      901 gaacgggatc gggatggtgc gggagacgat caccggcttc aaccgccgcc gggccatgct
      961 ccggaaagtc cggggccgac agcgcgtgct ctttctcacc ggcgagtcgg cccgaccctt
     1021 tctcgagtcg cacgtggccg cgtacgcccg ggatcaattg gggctgcggc tggaggtcga
     1081 ggcggtcaag aacgaattct ggggtccgac cgtcaccgtg tcgggcctgc tcaccggaca
     1141 ggatctcctg cggcacgcgc gccggcggca cgaaaactgg gacgtgctcg tcgtgccgcc
     1201 caactgcctg aacgacgacg atctgttcct ggacaacctg agcctggagc agttccgggg
     1261 cgtgctggcc aaaccggtgc tggtcgggca atacaacgtc gcggcatcgc tgaaagaggc
     1321 cttctcatga aactcccgac cgtggccatt gtcggacgcc cgaacgtggg gaaatcgtcg
     1381 ctcttcaacc ggtttctccg caaatacctg gccgtggtgg atgaaacccc cggcgtcacc
     1441 cgcgatcgca actacgcggt gtgcgactgg gccggtcggg agttccgcct gatcgacacc
     1501 gggggcctgg tccccaagtc cggcgatctc atgaaccggc tgatctacga gcaggccgat
     1561 ttcgccatcc acgaagccga cctcgtgctg ctggtggtcg atgccatggt cggcgccgac
     1621 tacatcgatg aagaaatcgc ccgccgcctg cagcagtccg ccaaacccac catcctggtg
     1681 gccaacaaag ccgacaatcc ccggctggag ttggaggtgt atgatttcct ccgcctcggc
     1741 ctgggcgagc cgatggcggt ctcggccacc gtcgggcgcg gtatcggcga gctcctcgac
     1801 aaaatcgtgg cgctgctgcc ggaggcgccc gcggacgacg tcggcggttc cggcgccgtc
     1861 cgcgtggcag tcgtggggcg gccgaacgtg ggcaagtcct cgttcatcaa caaactcacc
     1921 ggagaagagc gggccattgt gaccccgatc gcgggaacga cgcgcgatgc cgtggatacg
     1981 ccgttttcct tcgaagggcg gaactacatt ctcgtcgaca ccgccgggct gcgccggaag
     2041 ttcaaagttc acgaggatat cgaattctac acgacgctgc ggacgagccg ggcaatcgac
     2101 agcgccgagg tcgccgtagt gctgatcgac gcgcaggacg gcgtgacgtc gcaggaccag
     2161 cgcattctcg aggatgttgt tgaaacgcgc tgcgccgcgg tgctggcggt caacaagtgg
     2221 gacctgatcg agaaggagac gggcaccgcc gaacgctttg cgcggcagat taacgaggcg
     2281 ctcgcccact actcctatct gccgctcgtt ttcatctcgg ccttgaccgg acagcgggtg
     2341 ggcaaagtgc tggcgctggt cgaccaggtg cacgcggaga atacgcggca gctgcccacg
     2401 cccgaactca acgactggct cgccgaggtg acggcgcggc gccatccccc ggcgcggcag
     2461 ggcaaacaca tcaagttcaa ttacatcacc cagaccggcg cggccccgcc ctcctttgtc
     2521 atcttcgcca attaccccaa gctcgtcgac aggtcgtacg tgaattacct gcacaaccaa
     2581 ctgcgggagc gctggggatt tgcgggcgtg tcgctccggc tgaaattccg ccgcaagtag
     2641 caaccgcccc gcgccgattg gcacccgacg ctgtcgcaca gccccctgag tcaattcacg
     2701 cccggccgct gccgataatc ccggtatgga aacccggccg gtgtacgatg tcgacccgga
     2761 gcagctggcg gcctcgctgg cggcccggat cgccgacctt caggatctcg ccaccatggg
     2821 cgcggtgatc gcgtccattc acgacatcga cgccatcctc tcggtcgtca tggacatgtc
     2881 gctgcggctg gtcgatggcg aggtgggcgc gatcctgctg gagaaaagcg ggcagttggg
     2941 tatcgaaatc tcgtggggcg tccaggaggg gctcatcccc aaactcccgg gcggcgagac
     3001 ggccgaagaa ctgctgcccg ccggggaagc cgcccctccc ggcggtatgg acctgcccac
     3061 ccgcgtgctg gcctccgggc agacggtgat cctcaacgac ctcagccttc gcgatgaaga
     3121 cgggcggcgc atcgacgccg tcatctgcat gcccatcggg accagggacc ggcgctacgg
     3181 cgtcctgctg attctcaacc gggccggcgg cggtggcttc aacgacgagg atcgggagcg
     3241 cctggccatg ctcctcaatt tcgtggccgt ggcaatcgac aacgcccgcc tcatgcagga
     3301 gcagctcagc cgtcagcgga tcgaacagga gatggcgatc gcacgccagg tccaggaaac
     3361 catcctcccg caggacatcg atgcggtcgg cggcgtggaa atcggcgcct cctactaccc
     3421 cgcgcaggaa gtgggcggcg acttctacga cgtccacgcg ctgtcggata acgagttcct
     3481 cgtcttcatc ggcgacgtct ccaacaaggg ggtgccggcg gcgctggtga tgtcggcggc
     3541 tgcgggaatc atcaaatcca tcctcgaccg cagccccgag atcgcggtca atgaactcgc
     3601 ctcccgcctg aacgagattc tcgcacgcga gatcatccgc gaccgcgaga tgtttatcac
     3661 cctcttcttc gcccgcttcg atctcgcccg gggccggatg acctactgca acggcggcca
     3721 cgtcccgggg atgttctggt cggcccggga ggggacggtg gccaggctgt cgaccggcgg
     3781 cccgattgtc ggccagtttc cgggagcgca ctttgatctc ggttcgtgcc cgatcggttc
     3841 gggcgaccgc ctgtttttgt gcaccgatgg gctcaccgag gccgtcgaca aagacggacg
     3901 gatattcgga cgggaacagg cggaagaagt gttcgcgatg gagatcggcc ttcccccaaa
     3961 agagttctgt gttcgcatac gggagtggat tgaccggttc gcgtcaggcg cgccggaaga
     4021 ttcgcacgac gatttcaccg ttttgcaggt gctggtgaag tgatggcgac acatcggttt
     4081 gcctacccct ccgtggttga atccgaggag cggtttctca acgatctggc gcaggtggca
     4141 caacgggaga ggcttgagga ggacgtgcga cgggcgatga tgttgacctt gtccgaggcc
     4201 ttcaccaacg ctctggtcca cggcaaccgg ctcgatccgg ccaagaccgt gtgggtggag
     4261 gtccgggtgg acgaggcgac ggtctccgcg gaggtggccg atgagggcaa ctgcggactg
     4321 gccgaactcg ccgcgcggcc ccaaaccggt cagctcgacg accacgggcg ggggatcggg
     4381 ctgatggagc actttgccgc ggaagtggtc ttcggcgaga cccttaccgg cggccttaaa
     4441 gtatcggtcc gattccgccg actagtagag agaacaaggg gatgaggaga agcggcggcc
     4501 ggttccggac ccgcgggagg ccccggggta cggcgatccg gctgcaagaa accattcgtg
     4561 cgggatgtac tggaggcgcg atgactataa cagtcgaaac tttcggaaat gtggcggtgc
     4621 tccgactgaa cgggcgcctg gatctggcca gcctgctcgc tgccttggcg gtggagctgg
     4681 tgcag
//
LOCUS       DAOOKS010000046         4683 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_22044_length_4683_cov_3.294512, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000046 DAOOKS010000000
VERSION     DAOOKS010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4683
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22044_length_4683_cov_3.294512"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="PK112_01355"
     CDS             complement(<1..723)
                     /locus_tag="PK112_01355"
                     /inference="COORDINATES: protein motif:HMM:NF012449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HPC10726.1"
                     /translation="MKHTKILATFGPAIDDPDRLRELVSAGVNLFRVNCSHGESADFC
                     RAAATIRQATARACFPIGLLFDISGPKLRLDRFEGSLDIAEGTELTLVADRTDLAHRL
                     IAVNHPAVIASLRVDDRLFMDDGQFLFHVTARTEQAVTVRSHSSGKLLGGKGINLPGV
                     PLPIPTITAKDRQDIETAVRCGADYIALSFVRSADDVVQARELIAAAGGGPKVIAKLE
                     KREAIDRLDEIMSAADGVMIARG"
     gene            984..1352
                     /locus_tag="PK112_01360"
     CDS             984..1352
                     /locus_tag="PK112_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10727.1"
                     /translation="MSKRVIIAANNMIFTSKIMTALDSLKAEGIKAIRSEEIFSKAQD
                     LQPDLIIIDLNLNGIEAPALINKLRSYGATKQIPIVCYSPNLLKDQMKAAKVAGATEV
                     YPNSQMTSRMNQILGKYIQN"
     gene            1592..3172
                     /locus_tag="PK112_01365"
     CDS             1592..3172
                     /locus_tag="PK112_01365"
                     /inference="COORDINATES: protein motif:HMM:NF025341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HPC10728.1"
                     /translation="MGSLVLTAAAVVVAAAAGAVDVDSLLVQSVGGPEAYRTLAAMTS
                     YRTEGKADINGLEGTYTQIYAAPDRFYMEIRIGGMTLVSAFDGRTAWQRDHNGRVSEV
                     EGMEKRQLIEQVYLASYSFIISGRLPGRSEFLGDTTIADTSWLRVALYPRPDDTVVML
                     LDGQSALPAYVVQRLDNLTGVTRLSDYRTVAGVALAHSARTEFPEAALYTEMTTESVA
                     FNVEVDPALFDLPGAEPVDYRFAAGADSVVIPFTYYRGHVWLPAAVNGATVAWFILDS
                     GASANLLNDALTAPLGLTSVGSVPAKGVGGFEEVPLVRTDSITIGSLTLVDQIAAAMD
                     WDRLGIEPPPGAQAGGILGHDFLSRFPVLIDYSDSTLTAYNPAAFTAPAGGTAVDFHL
                     TMLVPTISASVGGVEGDFIIDLGNSVGLVLHDRFFQDHRLDTVLTNLASVDRPIGGVG
                     GTARTRSAVVPAFRIGGMVLSDVPVVIPESTQGLAGSTELAGNIGNLVLQEFRVLLDY
                     RASRVYFYPPGAREGVAR"
     gene            3169..3918
                     /locus_tag="PK112_01370"
     CDS             3169..3918
                     /locus_tag="PK112_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10729.1"
                     /translation="MTVHGRRGRGGIAALVFAVLAAAAWGGARAAGEEPTTSQLFLQS
                     HQAGVRLGPWFNLGDMPPARGETSGGEEVFKTNFHDASFLFEGFYAHRLTPALMGELS
                     LAILNRGSVTFTAGNQTNVGNVLVYAGLLQAKVYPLAGGGASVQPYLTAGGGLYVGRR
                     SVQFTSVPEGYYYPNIDEETAAKISYAVGGGIDWPIASQIGLEINAKYCPIPFGKPLM
                     TIEDYDGLAVSIGIKYLYRPAKDETDSRRRP"
     gene            3915..>4683
                     /locus_tag="PK112_01375"
     CDS             3915..>4683
                     /locus_tag="PK112_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HPC10730.1"
                     /translation="MRVAINGFGRIGRLVFRAAQDTSIEFVAINDITDAATLGHLLKY
                     DSVHRSYPGEVKAEGTNLKVDGRTIPVMAVLDPAQLPWKELKVDVVLECTGKFTKKDD
                     AMKHVAAGAHKVLISAPAKGHDGTFIPGINSDLYDRDKHHVISIGSCTTNCLAPVAKV
                     LLESFGIVNGFMTTIHSYTADQRLMDAPHKDLRRARSAALSMVPTTTGAAKAIAEVIP
                     ALKGKMDGCAIRVPTPNASLVDLAVTLEREATVQEINA"
BASE COUNT          850 a         1447 c         1560 g          826 t
ORIGIN      
        1 cccccgcgcg atcatcaccc cgtcggccgc cgacattatc tcgtcgagcc ggtcgatcgc
       61 ctcgcgcttc tcgagcttgg cgataacctt cgggccaccg ccggcggcgg cgatgagttc
      121 ccgcgcctga acgacatcgt cggccgagcg cacaaacgag agcgcgatat agtcggcgcc
      181 gcagcgcacc gcggtctcga tatcttggcg gtccttggcg gtgatcgtgg gaatcggcag
      241 gggcacgccg ggaaggttaa ttcccttgcc gcccaataac tttccggagc tgtgcgaccg
      301 cacggtcacg gcctgttcag tccgtgcggt cacgtgaaag agaaactgac cgtcatccat
      361 gaacagccgg tcgtcgacac gcagcgaggc aatcacggcg ggatggttga ccgcgatcag
      421 tcgatgggcc aggtctgtcc ggtcggcgac cagggtgagc tcggtcccct cggcaatgtc
      481 gaggctcccc tcaaagcgat ccaggcgcaa tttgggcccg gaaatatcga acaacaggcc
      541 gatcgggaaa caagcccggg cggtggcctg gcggatcgtg gcggcggccc ggcagaaatc
      601 ggcggactcg ccatgggagc agttgactcg gaacaggttg acgccggccg agacgagctc
      661 ccgaagccga tcgggatcgt cgatggctgg accgaaggtg gccagtatct tcgtatgttt
      721 cacgaacttc ttagtacggg ataagaatcg ggaaatcaac aaaaaggcgc agccgcagac
      781 gggcggggaa ggtcgagggc atcacggatg gcggagggtg ccaccggcgg catgtgatct
      841 atctggctga caaccagtta ccacttttct acggctcccg gtttgacttc gcccgccgga
      901 tagtgtatat tgccccggtc aggggttacc ggggatggct cttccctggg ttagggatga
      961 aaccgtaaca atggattgac acagtgagta agagagtcat tatcgctgcc aacaacatga
     1021 tcttcacctc caagatcatg acggcgctgg attctctcaa ggccgagggg atcaaagcca
     1081 tcagatcgga ggaaatcttc agcaaggccc aggacctcca gcctgatctc atcatcatcg
     1141 acctgaatct gaacgggatt gaagcgccgg cgctgatcaa caagctccgc tcgtacggcg
     1201 ccaccaagca gatcccgatc gtgtgttatt cgccgaacct gctgaaggac cagatgaaag
     1261 cggccaaggt ggccggcgcc accgaggtgt atccgaactc ccagatgacg tcgcgcatga
     1321 accagattct gggcaagtat atccagaact gacgatcgga gggggccggt cacacgaccg
     1381 gcgggcgggc agtcttcccc cgttcggcag cggcgctgac agatcgcggc atcatccccg
     1441 gccgtccgac gggccgcgga tcttccggga aaatcatcgc cgcaccggca ccattccgga
     1501 ttcgattttc ccggcgacat tttagctttc gcccggcgcc gatttagcgt atacgtgcat
     1561 cgtggacagg tgtacaagac aacggaccgt tatgggcagc ctggtgctga cagcggcggc
     1621 agttgtcgtc gcggccgcgg ccggagcggt cgatgtggat tcgctcctgg tgcagtcggt
     1681 cggcgggccg gaggcgtacc gcacgctcgc cgccatgacc tcctaccgga cggaggggaa
     1741 ggccgatatc aacggtctgg aggggactta cactcagatc tatgccgccc cggaccgatt
     1801 ctatatggaa atccggatcg gcgggatgac gctggtgtca gcctttgacg gccggaccgc
     1861 ctggcagcgc gaccacaacg gccgggtgtc ggaagtggaa gggatggaga agcgccagtt
     1921 gattgaacag gtctacctgg cctcgtattc atttatcatc tccggtcgtc tgcccggccg
     1981 ctccgagttt ctgggggaca cgacgatcgc cgacacctcg tggcttcggg tcgccctcta
     2041 tccccggccg gacgacacgg tcgtcatgct gctggacggg cagtcggccc tgccggcgta
     2101 cgtcgtgcag cggctcgata acctcacggg cgtgacgcgg ctgtccgatt accggacggt
     2161 tgcgggggtg gcgctggcgc actccgcgcg gaccgaattc cccgaggcgg cgttatatac
     2221 ggagatgaca accgagagcg tggcgttcaa cgtcgaggtc gatcccgcgc tgttcgacct
     2281 gccgggggcg gaaccggtcg actaccgctt cgccgcaggc gccgattccg tggtgattcc
     2341 tttcacgtac taccgggggc acgtgtggct gcccgccgcg gtcaacggcg cgacggtcgc
     2401 ctggttcatc ctcgattccg gggcctcggc caacctgctc aacgatgcgc tgaccgcgcc
     2461 gctgggactg acatcggtcg ggtcggtccc cgccaaaggg gtaggcgggt tcgaggaagt
     2521 ccctctggtg cggaccgact ccattacgat cggctcgctc accctggtgg accagattgc
     2581 cgcggcgatg gactgggacc ggctggggat cgaaccgccg ccgggcgccc aggcaggggg
     2641 tatcctgggc catgacttcc tgtcccgttt tccggtgctg attgactact ccgattcgac
     2701 gctgaccgcg tacaacccgg ctgccttcac cgcgcccgcc ggtggaacgg cggtcgattt
     2761 tcacctgacc atgctcgtcc cgacgatttc ggcgtcggtc ggcggcgtcg aaggagactt
     2821 catcatcgac ctcggcaact cggtcgggct ggtgctgcac gatcggtttt ttcaggatca
     2881 ccgtctcgac acggtcctga ccaacctcgc ctccgttgat cggccgatcg gcggggtggg
     2941 gggaaccgcg cgcacccgct cggcggtggt gccggcgttt cgcatcggcg gcatggtgct
     3001 gtccgatgtg ccggtggtca ttcccgaatc gacccagggg ttggcgggga gtacggagct
     3061 ggcgggcaat atcggcaatc tggtgctgca ggagtttcgg gtgctgctgg actaccgcgc
     3121 gagccgggtg tacttttacc cgcccggcgc gcgggaagga gtcgcacgat gacggtgcac
     3181 ggccgacggg ggcgcggcgg gatcgcggcg ctcgtgttcg cggtgctggc ggccgcggcc
     3241 tggggcgggg cgcgggcggc cggcgaggaa ccgaccacct cgcagctctt cctccagtcc
     3301 caccaggcgg gcgtgcggct gggcccgtgg ttcaacctcg gcgacatgcc gccggcccgc
     3361 ggggagacca gcggcggcga agaagtgttc aagaccaatt ttcacgacgc cagtttcctt
     3421 ttcgaggggt tttacgccca ccggctcaca cccgcgctca tgggcgaact ttcgctggcg
     3481 atcctcaacc gcggctcggt cacgttcacc gccggcaacc aaaccaacgt gggcaacgtg
     3541 ctggtgtacg ccgggctgct gcaggcgaag gtgtatccgc tggcgggggg cggcgccagc
     3601 gtgcagccgt atctgacggc cggcggcggg ctatatgtcg ggcggcgctc ggtgcagttc
     3661 acctcggtcc ccgagggcta ctactacccg aatatcgacg aggagacggc cgcgaagatc
     3721 agctatgcgg tcggcggcgg catcgactgg ccgatcgcct cgcagatcgg cctggaaatc
     3781 aacgccaagt attgtcccat tcctttcggc aagccgctca tgacgattga ggactatgac
     3841 ggccttgccg tttccatcgg aatcaaatat ctatatagac cggcaaaaga cgaaacggat
     3901 agcaggagga gaccatgaga gttgccatca acggattcgg acgtatcggg cgcctcgtgt
     3961 ttcgggccgc tcaggacacc agcattgagt ttgtcgcgat taacgatatc accgatgccg
     4021 ccacactcgg acacctgctc aagtacgatt ccgtccaccg gagctacccc ggcgaggtga
     4081 aggccgaggg gacgaacctg aaggtcgacg ggcggacgat tccggtcatg gcggtgctcg
     4141 acccggccca gttgccgtgg aaggagctca aagtcgacgt ggtgctcgag tgcacgggga
     4201 agttcacgaa gaaggacgac gcgatgaagc acgtcgcggc gggcgcccac aaggtgctca
     4261 tctcggcgcc ggccaagggc cacgacggca cctttatccc ggggatcaac tcggacctct
     4321 acgaccgcga caagcaccac gtcatttcga tcggatcgtg cacgaccaac tgcctggccc
     4381 cggtggcgaa ggttctgctg gagagtttcg ggatcgtcaa cgggttcatg acgacgatcc
     4441 actcgtacac ggccgaccag cggctcatgg atgcgccgca caaagatctg cggcgggcgc
     4501 gctcggcggc cctgtcgatg gtgccgacga ccacgggcgc ggccaaggcg attgccgagg
     4561 tgatcccggc gctcaagggg aaaatggacg gctgcgccat ccgcgtcccc accccgaacg
     4621 cctcgctggt ggatctcgca gtgacgctgg agcgggaggc cacggtgcag gagatcaacg
     4681 cgg
//
LOCUS       DAOOKS010000047         4666 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_22153_length_4666_cov_3.254609, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000047 DAOOKS010000000
VERSION     DAOOKS010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4666
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22153_length_4666_cov_3.254609"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      1..124
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            149..1309
                     /gene="ribD"
                     /locus_tag="PK112_01380"
     CDS             149..1309
                     /gene="ribD"
                     /locus_tag="PK112_01380"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HPC10731.1"
                     /translation="MPPGGPCASAPKLCIRGFFVADETDIRYMQRALELAARGRFRTA
                     PKPMVGAVIVRQGRIIGEGYHRKAGSDHAEIAAIRNAVEPVAGSTVYVTLEPCCHTGH
                     TGPCTAALIEHGVARVVFAATDPDPRVRGKGARRLRQAGIRVEAGILREQAVRLNEVF
                     FAFHRTGRPFIILKTAQTLDGRIGAGNGDSKWISSPASRRMAHELRAEADAIVVGGGT
                     VRRDNPALTVRHVTGPDPYRVILSASLRFPKSCRLLDDNADARTIIATMAENVERFAR
                     TRRGRSLTFWSIRRRRDGQLDVADLVRKAGQFGLRSLLVEGGSRVATSFLKAGLVDKY
                     VAFVAPKLLGTGIDAVGDLGIASIDRALTFARTEILTCGPDIVVIGYPKRRK"
     gene            1311..1946
                     /locus_tag="PK112_01385"
     CDS             1311..1946
                     /locus_tag="PK112_01385"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HPC10732.1"
                     /translation="MFTGLVEDVGTIADIGLRGNYRVLSIVSVLADEHLRIGDSVNCD
                     GACLTVVRVGAGTFTVEASPETAARTILNDYRRGDRLNLERALRAGDRLGGHFVLGHV
                     DTTGTIEHARSAGESREIAVTFDPAFDALVVEKGSIALDGISLTVNAVRSAWLAVSLI
                     PHSLARTTAGGYQAGRRVNIEFDMIGKYIIKIRQSTGSNTLTLDKLRQSGW"
     gene            1950..3176
                     /locus_tag="PK112_01390"
                     /pseudo
     CDS             1950..3176
                     /locus_tag="PK112_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050493.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
     gene            3180..3650
                     /gene="ribE"
                     /locus_tag="PK112_01395"
     CDS             3180..3650
                     /gene="ribE"
                     /locus_tag="PK112_01395"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HPC10733.1"
                     /translation="MAYKTYEGKLEAAGFRFGLVVSRFNNFLTDKLLEGALDCLMRHG
                     ASEDKIAVAYCPGGFEVPPVAARMVRSGKYDAVVCLGAVIRGDTPHFEYIAAESAKGI
                     ARLAMEAGIPVIYGVVTADTLEQAIERSGTKAGNKGWHAAEAALEMVNLYRAIE"
     gene            3694..4131
                     /gene="nusB"
                     /locus_tag="PK112_01400"
     CDS             3694..4131
                     /gene="nusB"
                     /locus_tag="PK112_01400"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HPC10734.1"
                     /translation="MRPFEQPRRQARELVLQALYATEFGSARPEEALADLLADQPLAE
                     GATAFAQRLFASTRRRVEWANAAIAELAVNWNINRIAAVDRVILRMALVELDEFPDTP
                     VKVVLNEAIELAKKFSTAESSAFVNGILDAWVKRNDRGTGPGT"
     gene            complement(4162..>4666)
                     /locus_tag="PK112_01405"
     CDS             complement(4162..>4666)
                     /locus_tag="PK112_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10735.1"
                     /translation="EGIEGRSRKRQLELAAAFGLEDYPTPAAGCLLTDVGYSNRLRDL
                     MAHTDRLTFDELNLLKYGRHFRLAPDAKIVVGRDEEDNRHLLALKRPQHVHLEALDTN
                     SPVTLLAGRDRSEEILRTAAAITARYSDAKHAAAVAVTIFEEGAAPRAISVAPAADAD
                     IQLLILK"
BASE COUNT          822 a         1384 c         1714 g          746 t
ORIGIN      
        1 aacagtcttc ggggcgaggc gaaaatcctc gaccggcggt gagagcccgc gactcccacg
       61 agagtgggac tgagcctgtg aaattcaggc gccgacggta cagtccggat gggagaagat
      121 tgcgattcga ccggcggtgc ggccgtgtat gccgcccggc ggcccgtgcg cctcggcccc
      181 gaaactctgc attcggggtt ttttcgtggc cgacgagacg gacatcaggt acatgcaacg
      241 ggcgctcgag ctggcggcgc ggggacggtt ccgaaccgcg ccgaagccga tggtcggggc
      301 ggtgattgtc cgccaggggc gcatcatcgg cgaggggtat caccggaagg caggctcgga
      361 tcacgccgaa atcgcggcca tccggaacgc cgtcgagccg gtggccggga gcacggtgta
      421 cgtgacgctg gagccgtgct gccacaccgg gcacaccggt ccctgcacgg cggccctcat
      481 cgagcacggc gtggcgcgcg tcgtgttcgc cgccaccgat cccgaccccc gcgtgcgggg
      541 aaagggggcg cggcggctcc ggcaggcggg tatccgggta gaggccggga tcctgcgcga
      601 gcaggcggtt cggctcaacg aagtattctt cgccttccat cggaccggcc ggccgttcat
      661 tatcctcaag acggcgcaga cactcgatgg ccggattggg gcgggcaacg gcgactcgaa
      721 atggatttcc agtccggcct cgcggcggat ggcccacgaa ctgcgggccg aagcggatgc
      781 gatcgtagtg gggggcggaa ccgtgcggcg cgacaacccc gccctgaccg tgcgccacgt
      841 caccggaccc gatccctacc gcgtcatcct cagcgcctcc ctgcgtttcc ccaaatcctg
      901 ccgccttctg gacgacaacg ccgacgcccg cacgatcatc gcgacgatgg ccgaaaatgt
      961 cgagcggttt gcgcggaccc ggcgcggacg ctcgctcacc ttctggtcga ttcggaggcg
     1021 ccgggatggg cagctcgacg tggccgacct ggtgcgcaag gcgggccaat tcggtctgcg
     1081 ctcgctgttg gtcgaggggg ggagccgggt ggcaacgtcg tttctcaaag ccgggctggt
     1141 cgacaagtat gtcgcgttcg tggcccccaa gctcctcggc acaggaatcg acgccgtcgg
     1201 cgatctcggc atcgcctcga tcgacagggc gctgaccttc gcgcgcactg aaatactcac
     1261 ctgcggcccg gacatcgtcg tcatcgggta tccgaagagg aggaagtgag atgtttaccg
     1321 ggttggtcga agacgtgggg acgatcgccg acatcggcct gcgggggaac taccgggtgt
     1381 tgtcgatagt ctcggtgctg gccgatgagc atctgcggat cggcgattcg gtgaactgcg
     1441 acggcgcctg cctgaccgtg gtccgggtcg gggcggggac gttcacggtg gaggcgtcgc
     1501 ccgagacggc ggcgcggaca attttgaatg actaccggcg cggcgaccgg ctcaatctcg
     1561 aacgggctct gcgggcgggc gaccggctcg gcgggcattt cgtcctcgga catgtcgaca
     1621 cgaccgggac aatcgagcac gcccgatcgg cgggggaatc gcgggagatc gccgtgacct
     1681 tcgacccggc cttcgatgcg ctcgtggtcg agaaagggtc gattgcgctt gacgggattt
     1741 cgctgacggt gaatgccgtg cgatcggcct ggctggcggt atctctgatt ccgcacagcc
     1801 ttgcccggac gaccgcgggc ggatatcagg cgggccggcg ggtgaacatt gaattcgata
     1861 tgattggcaa atatatcatt aagatacggc aatcaaccgg aagtaatact ttaaccttgg
     1921 ataagctcag gcagagcgga tggtaagcca tggcggacga gcagatattc aacacggtgg
     1981 aagaagccat ccaggagatt cacgcggggc ggtgtgtcat agtcgccgac gatgaggacc
     2041 gcgagaacga aggggacctg atcatggcgg ccgagaaagt gacgccggag tgggtcaact
     2101 tcttcgccaa acacggccgg ggtctgattt gtgtctgcat gcccgaggag cggatcgccc
     2161 ggctcgcgct gcacccgatg gtggatcgga acacggcgct ccacggcacg cggttcacgg
     2221 tgtcggtgga cgcggtggag gggacgacga ccgggatcag cgcggccgac cgggcgaaaa
     2281 cggtgcgggt gctggccaat ccagcctcgc ggccggagga tctggcgcgg ccgggacaca
     2341 tctttcccat ccaggcggtg accggaggcg tcctgaagcg cgccgggcac accgaaaccg
     2401 cggtggatct ggcgcggctg gcgggtctga acccggtggg gatgctgtgc gagattatgg
     2461 atgacgacgg gagcatggcg cgggtacccc ggctggcgca gttcgccagg gagcacgggc
     2521 tggcgttcat taccgtgcgc gacctcatcg cctaccgccg ccggaccgaa aagaacgtcg
     2581 agcgcgtgga gaccgtcgac ttccccaccg aataggggga tttccgcctt cacctgtaca
     2641 agtcgaaact cgatgaccac taccacgtcg ccctggtgaa aggggaagtg gcggggaaaa
     2701 aagacgtgct cgtccgggtc cactcgagct gcgtgaccgg ggacattttc gggtcctgcc
     2761 ggtgcgactg cgggcagcag ctccacgcgg cgatgcgcca ggtcgaccgg gaaggctgcg
     2821 gcgtcgtgct ctacatgacg caggaggggc gcgggatcgg gttcgccaac aagatccgcg
     2881 cctaccggct tcaggaggac gggcaggata cggtggaggc gaacgaatcg ctcgggttca
     2941 agccggatct ccgggagtac ggcgtcgggg cgcagattct ggttgatctg gggctgagtt
     3001 cgatccgact attgacgaac aatccgaaga aggtgatcgg gttggaaggg tacggtctgg
     3061 agatcaccga gcgcgtgccg ctggagttcg cgcccggaaa atacaaccgc cgctatctgg
     3121 aagcgaagcg ggacaagctg ggacactttc tctcactgaa acggatggga gcgtagcgta
     3181 tggcctacaa gacctatgag ggcaagctgg aggcggcggg ctttcggttc gggctggtgg
     3241 tgagccggtt caacaatttc ctgaccgaca aactgctcga gggcgccctt gattgcctga
     3301 tgcggcacgg ggcgagcgag gataaaatcg ccgtcgccta ctgcccgggc ggattcgagg
     3361 tgccgcccgt cgcggcacgc atggtgcggt cggggaagta cgacgccgtg gtctgcctgg
     3421 gcgcggtgat ccgcggcgac acgccgcact tcgagtacat cgccgccgag tcggccaagg
     3481 gaattgcccg gctggcgatg gaggcgggga tcccggtgat ctatggcgtg gtgacggcgg
     3541 acacgctcga gcaggcgatc gaacgctcgg ggaccaaggc ggggaacaag gggtggcacg
     3601 cggccgaggc ggcgctcgaa atggtgaatc tctacagggc gatcgaatga agcgggcggc
     3661 ggcggagaac gccgcaggcg acgggggggc cgcgtgagac cgttcgaaca accccgccgg
     3721 caggcgcgcg aactggtgct ccaggcgctc tacgccaccg agttcggcag cgcgcggccg
     3781 gaggaagcgt tggccgacct gctcgccgac cagccgctgg ccgaaggggc gacggccttt
     3841 gcgcagcgtc tgttcgcctc gacgcggcgc cgggtcgaat gggccaacgc cgccatcgcc
     3901 gagctggcgg tcaactggaa tatcaaccgc atcgcggccg tcgaccgggt gatcctgcga
     3961 atggcgctgg tggagctgga tgagttcccc gatacgccgg tgaaggtggt gctcaacgag
     4021 gcaatcgaac tggcgaagaa gttctcgacc gccgaatcgt cggccttcgt caacgggatc
     4081 ctcgatgcgt gggtgaagcg gaacgaccgg ggaacgggcc cggggacgta gggcgagcgg
     4141 ccggcggcga agtctgtccc ttcacttgag aatcagcaat tggatatcgg cgtcggcggc
     4201 cggtgcgacg ctgatcgcgc ggggcgccgc accctcctcg aaaatcgtga ccgccacggc
     4261 ggcggcgtgc ttggcatcgg agtaacgcgc cgtgatcgcc gccgcggtgc ggagtatctc
     4321 ctccgaccgg tcccggccgg ccaggagggt cacgggggag ttggtgtcga gggcctcgag
     4381 gtggacgtgc tggggccgct tgagcgcgag cagatgccgg ttgtcctcct cgtcgcggcc
     4441 gaccacgatt ttggcgtcgg gcgcgaggcg gaagtggcgg ccgtacttga gcaggttcag
     4501 ctcgtcgaaa gtcagccggt cggtgtgcgc catgaggtcg cgcaggcgat ttgaatagcc
     4561 gacgtcggtg agcagacagc cggccgcggg cgtggggtaa tcctccagac cgaatgcggc
     4621 ggccagctcg agctggcgtt tgcgggaacg cccctctatc ccctcg
//
LOCUS       DAOOKS010000048         4598 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_22593_length_4598_cov_3.445741, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000048 DAOOKS010000000
VERSION     DAOOKS010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4598
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22593_length_4598_cov_3.445741"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1178)
                     /locus_tag="PK112_01410"
     CDS             complement(<1..1178)
                     /locus_tag="PK112_01410"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPC10736.1"
                     /translation="MRKKRAERRVPLFDLAVSPAARRYVNDALASGWLTAGPKVKAFE
                     AAVARLTGVRHAAAVASGTQGLHLALAACGGVPGREVVTTPYTFVATVEAILLAGGTP
                     VFADIDPHTLTIDPDAAARKVGKKTAAVVPVDIAGHPCDYPRLNDLCGAHNLPLVADA
                     AHAIGGAYRRRAIPKLCDAAVYSFYSTKNLTAGEGGMAVSRHKELIDRIRLLARHGLT
                     SSTYERKLSNRWEYDAVAFGWKANMAELSAAVGLGEIERFGANQRARAAAAARYRRHL
                     AALAEYVVLPTEAPDCTHSWHLYIIKLRTEALRIGRDRFIREMAARGVECGVHYKPVY
                     ELSWYRRAFDLSARPFPNAEWAWRRAVTLPLYPTLAAADVDYVCAYRRNCAAISAMIF
                     R"
     gene            complement(1159..2226)
                     /locus_tag="PK112_01415"
     CDS             complement(1159..2226)
                     /locus_tag="PK112_01415"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPC10737.1"
                     /translation="MGARVVIFGWSGSVHVQRWVTGLQARGFEMKVVSLGGPPLADCE
                     TTVLPRTGRWSYLTHAARAAEAARAFAPDLVHAHYATGFGYWAVRSRLRPSLVSVWGA
                     DVIDFPAGWFRARLLRAVLRRATHLSATSQFLRSKVIGLDGSLASQTSVIPFGVAIPA
                     EAAAPPPAPPVRLCYVKNLRPKYGADVLLQAMARARKELPDLRLDIAGEGEMKARLLE
                     KTKNLGLDDLVEFVGFVPNDRVYAFIREHHIMVMPSVCLESFGVAALEAAACGRPVIA
                     SAVGGVPEVVRREETGILVPPGDSEKLAEAIVRLGRDAALRERFGRNGWAFAKENYNW
                     ELSLDRMSELYLRLIDEKKKS"
     gene            complement(2230..3786)
                     /locus_tag="PK112_01420"
     CDS             complement(2230..3786)
                     /locus_tag="PK112_01420"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HPC10738.1"
                     /translation="MTGGSMQRAVFGVAGVLLLSRVFGFVREMVIAHRFGTSADYDLY
                     LIAIMFSALAYGILNYAGYYLFVPYLTRRLQARAGAEGPPDFWPLVNTGAAAALAVTL
                     LLAAAGPALLRLWAVDYVGEGFDRIAFYSRVTAVVVVLGVAEAFMRAFLNVKRVYTHP
                     AAGYIVFNLFSILSIVLLAGRLGVGAIALGWIGGLLAQNVWLAARLVRVHAFGGYRPQ
                     MITEETKYLIATASALIVIELINRSYFLIDRYFAPQFGPGVISALNYSQVIVQLPDSI
                     IGWALGAVVFPMFSAHRGEADRAKFWFLYRRTVTAALVIAVPIAAALFITAPEVIYVL
                     LQRGEFDPQSTATTAAVLRPYVPSLIALFIVSTSIRACYSGGWVRAVFWATVSVFAVK
                     AAATWGLSALFGPGGISAATSAAEVVFAVLLLGIAVRRIAGEGRAAFLGVLGRILAAG
                     AAAAALAGLGQQLLPEWAAAGGRGPALIKIVLSGLAVAALYVLFGRLLGLRRQFADVL
                     ALRTQRAGEA"
     gene            complement(3875..>4598)
                     /locus_tag="PK112_01425"
     CDS             complement(3875..>4598)
                     /locus_tag="PK112_01425"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPC10739.1"
                     /translation="MPVHLFGQPADMDPICALARRHGLAVIEDAAQSIGAAYKGRPAG
                     SFGDYGCYSFYPSKNLGAIGDGGMIVTDRPENDQRVRILRVHGAEPKYFHKLVGYNSR
                     LATIQAAALLVKLPRLEAWSKERAAHARIYDEAFAGIEAIRRPAVKEYTTFHIFNQYT
                     IAVPNRDRVMEQLKAAGIGCEIYYPVPFHKQECFAYLGYRPGDFPVSSKAADEVLSLP
                     IYSEMTEAEQQEVIAAVRRILG"
BASE COUNT          784 a         1674 c         1513 g          627 t
ORIGIN      
        1 cggaatatca tcgccgagat cgcggcgcaa ttccggcggt aggcgcagac gtaatccacg
       61 tcggccgccg ccagggtcgg atacagcggc agcgtcaccg cccgccgcca cgcccactcg
      121 gcgttcggga aaggccgcgc cgagaggtca aaggcgcggc ggtaccatga gagctcgtag
      181 accggcttgt agtgcacacc gcattcgacg ccgcgcgccg ccatctcccg gatgaaccgg
      241 tcccgtccga tgcgcagcgc ctcggtgcgg agtttgatga tgtagaggtg ccagctgtgc
      301 gtgcagtcgg gcgcctcggt cggaagcacc acgtactcgg cgagagcagc aagatggcgg
      361 cggtaacggg cggcggccgc ggcccgcgcc ctctggttgg ccccgaacct ctcgatctcc
      421 cccagcccga ccgccgcact cagctccgcc atgttggctt tccacccgaa cgcgaccgca
      481 tcgtactccc agcggttgga cagcttccgc tcgtaggtcg agctggtgag cccatggcgg
      541 gccagcaggc ggatgcggtc gatcagctcc ttgtggcgcg acaccgccat gcccccctcg
      601 ccggcggtga ggtttttcgt ggaatagaat gaatacacgg cggcgtcgca cagcttgggg
      661 atcgcccgcc gccggtaggc gcccccgatc gcatgcgccg cgtcggccac gagcggcaag
      721 ttgtgcgcgc cgcagaggtc gttcaggcgc gggtagtcgc aggggtgccc ggcgatgtcc
      781 acgggaacca cggcggcagt cttcttcccc accttccgcg ccgccgcatc cggatcgata
      841 gtgagcgtgt gcgggtcgat gtcggcgaac acgggggtcc cgcccgccag gaggatcgcc
      901 tccacggtcg ctacgaacgt gtagggcgtg gtgaccacct cgcgtcccgg cacaccgccg
      961 cacgccgcca gggccaggtg cagcccctgg gtgccgctgg ccaccgcggc ggcgtggcgc
     1021 acaccggtga ggcgggccac cgccgcttcg aacgccttca ccttcgggcc ggccgtcagc
     1081 caccccgatg ccagtgcgtc gttgacatac cgccgcgccg ccggcgacac cgccaggtcg
     1141 aacagcggca cccgtcgctc agctcttttt tttctcatcg atcaaccgca ggtagagttc
     1201 cgacatccga tcgagcgaga gctcccaatt gtagttctcc ttggcgaacg cccatccgtt
     1261 ccgcccgaac cgttcgcgca gggcggcgtc gcggccgaga cggacaatcg cctcggcaag
     1321 cttctcggag tctcccggcg gcacgaggat ccccgtttcc tcgcgccgca cgacctcggg
     1381 cacaccgccg accgccgaag cgatcaccgg ccgcccgcag gccgccgcct ccagcgccgc
     1441 caccccgaac gactccagac acaccgacgg catcaccatg atgtggtgct cccggatgaa
     1501 agcgtatacg cggtcgttcg ggacaaaccc gacgaactcc acgaggtcgt cgaggcccag
     1561 gtttttcgtc ttctccagca gccgcgcctt catctccccc tcgccggcga tatcgaggcg
     1621 aaggtcgggg agttccttcc gcgcccgggc catcgcctgc aggagcacgt cggcgccgta
     1681 cttcggcctg aggttcttga catagcacag ccgcacgggc ggtgccggcg ggggggccgc
     1741 cgcctcggcc gggatcgcca ccccgaaagg gatgaccgac gtttgggaag cgaggctgcc
     1801 gtcgagcccg atcactttgc tcctcaagaa ctgactggtg gcgctgaggt gggtggcgcg
     1861 gcggaggacc gcacgcagca gccgcgcccg gaaccagccg gcggggaagt cgatgacgtc
     1921 ggcgccccac accgacacga ggctcggccg gaggcgcgac cgcaccgccc agtagccaaa
     1981 ccccgtcgcg tagtgcgcgt gcaccaggtc gggggcaaac gcccgcgccg cctcggccgc
     2041 ccgggccgcg tgggtgagat aggaccagcg accggtccgc ggcagcacgg tcgtctcgca
     2101 gtccgccagc ggcgggccgc ccagggacac caccttcatc tcgaatccgc gcgcctgcag
     2161 acccgtcacc cagcgctgta cgtgcaccga gcccgaccac ccgaatatca cgacccgcgc
     2221 gcccaccgcc tacgcctccc ccgcccgctg cgtccgaagg gcgagcacgt cggcgaactg
     2281 ccggcgcagt cccagcaggc gcccgaagag cacgtagagc gccgccacgg caaggcccga
     2341 gaggacaatt ttgatgagcg ccggcccgcg cccgcccgcc gccgcccact ccggcagcaa
     2401 ctgctggccg agaccggcga gcgcggccgc ggcggcgccg gccgccagga tgcggccgag
     2461 cacgcccaag aatgccgccc gcccctcgcc cgcgatccgg cgcacggcga tgcccaggag
     2521 caacacggcg aacaccacct cggccgccga ggtcgcggcc gagatcccgc cgggaccgaa
     2581 cagggccgac aacccccagg tggccgccgc cttcaccgcg aacacggaca ccgtcgccca
     2641 gaacactgcc cgcacccagc cgccggaata gcaggcccgg atcgaggtcg acacgatgaa
     2701 gagggcgatc agcgacggta cgtagggccg cagcacggcc gcggtcgtcg ctgtcgactg
     2761 cggatcgaac tccccgcgct gcaggagcac gtagatcact tcgggcgcgg tgatgaaaag
     2821 cgccgcggcg atcgggaccg cgatcaccag tgccgccgtc accgtgcgcc ggtagaggaa
     2881 ccagaatttc gcgcggtcgg cctccccccg gtgggccgag aacatcggga acacgaccgc
     2941 cccgagcgcc cagccgatga tggaatcggg cagctgcacg atcacctggc tgtagttgag
     3001 ggccgagatc acgcccggcc cgaactgcgg cgcgaagtag cggtcgatga ggaaatagct
     3061 ccggttgatc agctcaatga cgatgagcgc cgaggccgtc gcgatcagat acttcgtctc
     3121 ctcggtgatc atctgcggcc ggtacccgcc gaacgcgtgc acgcgcacca ggcgggccgc
     3181 cagccagaca ttctgcgcga gcaggccgcc gatccatccg agcgcgatcg cgccgacccc
     3241 gagccggccc gccaggagca cgatgctgag gatggagaac aggttgaaga cgatgtagcc
     3301 ggccgcaggg tgggtgtaga cccgcttgac attcaggaaa gcgcgcatga aggcctcggc
     3361 cacgccgagc acgactacga cggcggtgac gcggctgtag aaggcgatgc ggtcgaatcc
     3421 ctcgcctacg tagtcgaccg cccacaggcg gagcagcgcc ggtcccgccg ccgccaggag
     3481 gagcgtcacc gccagtgcag ccgccgcgcc ggtgttcacg agcggccaga aatccggcgg
     3541 cccctccgcc ccggcgcggg cctgcagccg gcgagtgaga tacggaacga agaggtagta
     3601 tccggcgtag ttgaggatcc cgtaggcgag agccgagaac atgatcgcga tcaggtagag
     3661 gtcgtagtcg gcgctggtgc cgaagcgatg ggcgatcacc atctcccgca cgaaaccgaa
     3721 cacccgcgac agaaggagca cgccggccac cccgaacacc gcccgctgca tcgacccgcc
     3781 cgtcatgccg ccgcctcccg ccgcctcggc tgttccgcct gatcctgtgc ctgcgcgctg
     3841 gtcataggcc ctgtagggac cgccccgccg ggcgctaacc gaggatccgc ctcacggccg
     3901 cgatcacttc ctgctgctcg gcctcggtca tttcgctgta aatcggaagc gacagcacct
     3961 cgtccgccgc cttgctcgaa accgggaaat cccccggccg gtagccgagg taggcgaaac
     4021 actcctgctt gtggaacggc accgggtagt agatctcgca gccgattccc gccgccttga
     4081 gctgctccat cacgcggtcg cggttgggga cggcgatggt gtactgattg aaaatatgga
     4141 acgtcgtgta ctccttcacc gccggcctgc gaatcgcctc gatcccggcg aaagcctcgt
     4201 catagatgcg ggcgtgggcg gcccgctctt tcgaccaggc ctcgaggcgc ggcagcttca
     4261 ccaggagggc cgccgcctgg atggtcgcca gccgcgaatt gtacccgacc agtttatgga
     4321 aatacttcgg ctcggcccca tgcacgcgca ggatgcgcac gcgctggtcg ttctccggcc
     4381 ggtcggtcac gatcatcccg ccgtcgccga tcgccccgag attcttcgag ggatagaacg
     4441 agtagcaccc gtagtcgccg aacgatccgg ccggccgccc cttgtaggcg gcgccgatcg
     4501 actgcgcggc gtcctcgatc accgccagac cgtgccgccg cgcgagtgca cagatcggat
     4561 ccatatcggc cggctgcccg aacaggtgga ccggcatg
//
LOCUS       DAOOKS010000049         4485 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_23367_length_4485_cov_3.086230, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000049 DAOOKS010000000
VERSION     DAOOKS010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4485
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23367_length_4485_cov_3.086230"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="PK112_01430"
     CDS             complement(<1..866)
                     /locus_tag="PK112_01430"
                     /inference="COORDINATES: protein motif:HMM:NF014589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
                     /protein_id="HPC10740.1"
                     /translation="MNILGLSCFYHDAAAALAVDGRLVAAAQEERFSRLKHDPELPVR
                     AVRWALDRTGLSTADLDAVAFYDKPILKFDRILTGCLAAPFRSWRPFLTALPVWLGRK
                     LWTDHIIRKELGFDGEVLYLPHHLSHAAGAFFTSPFSDAAVLTVDGVGEWATASYGVG
                     EGNRVRLIAEMHYPHSLGLLYSALTYYLGFRVNSAEYKVMGLAPYGKPRYADLIEDRL
                     VTVHADGSLHLRMKYFTFHRSLTMTGRAIERLFGCPRRAPGAPLEQFHADVAASLQAV
                     TEKIVLAMARHVR"
     gene            complement(868..1113)
                     /locus_tag="PK112_01435"
     CDS             complement(868..1113)
                     /locus_tag="PK112_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10741.1"
                     /translation="MKKVASYLWGRWRRAARVLGRAQTLALLAVLYALLLSPLGLLMR
                     LGGWDRLDVSARKAARTTNWKPVARPAPDREALRRQS"
     gene            1221..2297
                     /locus_tag="PK112_01440"
     CDS             1221..2297
                     /locus_tag="PK112_01440"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPC10742.1"
                     /translation="MATHKRILVTGASGSLGRQLLYEFTRRGCRPIAHVRPDSDTSYI
                     DSLGLEKRVADLGNEGQIEALVRDVDGIIHTAAWVNFRQDRLTQFAGVNTFGAVHVFQ
                     AAQKAGVERFVHVSTIAATAAIPRKNGEDGRPGGPRVRVTEETPFNLGHLRIPYFMTK
                     RAAEEELLKLAAGGATQLVIVNPAIIVAPSSTGDDRAKALKRFPRLVMPGFPNRLNLV
                     DIRDVAPAIIAALERGRPNERYILAGDNITARELVLSISQALGRVPHVMHVPRGVIEV
                     AARLWGIWVKLTGRGKLAFYPDLVKLLDYDWAYSSLKARRELGYTSRSVWVTLDDLLH
                     NRFLGTYLRPATEGKSEAAEKTPS"
     gene            2412..2819
                     /locus_tag="PK112_01445"
     CDS             2412..2819
                     /locus_tag="PK112_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10743.1"
                     /translation="MRKLVLFVVVVASLTGGPSRPAAGVIPSWESRTGKLGFMPLSNE
                     VTMLRSVDDAGGDRGWTLQLVLINSLKVFTTFKLEFTADYNFDYTPGQNRDHYVELSL
                     VKPVTGLVSLNYQRVLSTFEAEAVNQFGVRLAF"
     gene            2917..3999
                     /locus_tag="PK112_01450"
     CDS             2917..3999
                     /locus_tag="PK112_01450"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPC10744.1"
                     /translation="MSIRKTSNWCLLWAAAAAAAGCGDSPGDDAGRSGGNPGGAAMPV
                     NVIVVQPQPLDNKIFTTGTLLANEEVALRPEISGRVVGLYIEEGKAVKKGDLLVKLND
                     RELEAQLRRKQHEEALAAEEERRANALLDIKGISQEDYDRTLNKLRMIQAEREVIEAQ
                     LAETEILAPFDGTVGLRYISAGGVVTPSTLVATMQDTDPVKVEFSIPEKHAGKLAVKT
                     PVLVQVGDAPERHEGTVYAVEAKVDPATRTLKARATVPNPDGRLIPGSFAKVEITLEH
                     LPDALIVPAQAIVPQLGGDLVYVCREGAARATPVTVGLRGERTAQIIQGLAPGDTVIT
                     TGLLQLNDGKKVAARPEPADRSRTGL"
     gene            3996..>4485
                     /locus_tag="PK112_01455"
     CDS             3996..>4485
                     /locus_tag="PK112_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPC10745.1"
                     /translation="MNISSLCISRPVLATVLSILIVLFGGIGFFFLGVREYPNVDPPI
                     ISVSTAYTGANADVIESQITEPLEEEINGISGIRSLTSTSRDGRSSIRVEFELGVDME
                     AAANDVRDRVSRALGSLPKDVDPPIVSKADADATPILSMTLQSDRRSLLELSAIANDE
                     FKE"
BASE COUNT          792 a         1361 c         1616 g          716 t
ORIGIN      
        1 cgcacatgcc gcgccatcgc caggacgatc ttctccgtca ccgcctggag cgaggccgcc
       61 acgtcggcat ggaactgctc cagcggcgcg ccgggcgcgc ggcgcggaca gccgaaaaga
      121 cgttcgatgg cccgtccggt catggtcagg ctgcggtgaa aagtgaagta tttcattcgg
      181 aggtggagcg agccgtcggc atggacggtg acgagcctgt cctcgatcag gtcggcatag
      241 cgcggcttgc cgtacggggc gagacccatc actttgtact ccgccgagtt gacgcgaaag
      301 ccgaggtagt aggtgagggc cgaatagagc agaccgaggg agtgcgggta gtgcatctcg
      361 gcgatcagcc ggacccggtt gccctcgccc acgccgtagg aggccgtggc ccactccccg
      421 acgccgtcga ccgtgagcac tgcggcatcg ctgaacgggg aggtgaaaaa agcgccggcg
      481 gcgtgcgaga ggtggtgcgg gagatagagc acctcgccgt caaatcccag ttccttgcgg
      541 atgatgtggt cggtccagag cttgcggccg agccagaccg gcagggcggt caggaacggg
      601 cgccaggagc ggaaaggggc ggcgagacag ccggtcagga tgcggtcgaa tttgagaatc
      661 ggtttgtcgt agaaggccac ggcgtcgaga tcggctgtcg acagaccggt ccggtcgagg
      721 gcccagcgca cggcgcggac cggcagctcg ggatcatgct tgaggcggct gaaacgctcc
      781 tcctgggcgg cggccacgag acggccgtcg accgccagcg ctgccgcggc gtcgtggtag
      841 aagcaggata ggccgaggat attcatgtca gctctgccgt cggagcgcct cgcggtcggg
      901 cgcagggcgg gcgaccggtt tccaattggt ggtgcgggcg gctttccgcg cggaaacatc
      961 gaggcgatcc catccgccca gacgcatgag caggccgagc ggcgacagga gaagggcgta
     1021 gaggacggcc agcagcgcca gcgtctgtgc ccggccgagc acgcgggcgg cgcgcctcca
     1081 ccggccccaa agatacgatg cgactttctt cattttccgc gcgttaatct aataaaagct
     1141 gttgtgagag tcgagattct ttgcgtattt ccgggggtcc ggcggccgac gccgcggccc
     1201 ccaattagct cacgaagcag atggcaacgc acaagcgcat actggtcacc ggtgcttccg
     1261 gctcgctcgg ccggcagttg ctctatgagt tcacgcgccg gggatgccgg ccgatcgccc
     1321 acgtgcggcc cgactccgac acctcgtaca tcgattcgct cggcctggaa aaacgggttg
     1381 ccgacctcgg caacgagggg cagatcgagg cgctggtgcg ggacgtcgac gggatcatcc
     1441 acaccgcggc ctgggtcaat ttccgccagg accggctcac ccagttcgcg ggcgtgaaca
     1501 ccttcggagc cgtgcacgtc ttccaggcgg cgcagaaagc gggggtggaa cggtttgtcc
     1561 acgtgtcgac gatcgcggcg acggcggcaa ttccgcggaa aaacggggag gacggccgtc
     1621 cgggaggacc gcgagtgcgg gtgacggaag agacgccgtt caatctcggg cacctgcgca
     1681 tcccgtactt catgacgaag cgggcggccg aggaggagtt gctgaagctg gcggcggggg
     1741 gggcgaccca gctggtgatc gtgaatccgg ccatcatcgt cgcgccgtcg tcgacgggcg
     1801 acgaccgggc gaaagcgctg aagcggtttc cccggctggt gatgccgggg tttccgaacc
     1861 ggctcaacct cgtggacatt cgggatgtcg caccggcgat catcgccgct ttggagcgcg
     1921 ggcggccgaa cgagcggtat atcctggccg gggacaacat cacggcccgc gaactggtgc
     1981 tgagcatttc gcaggcgctc ggccgggtgc cccatgtcat gcacgtgccg cgcggggtga
     2041 tcgaggtggc ggcccggctg tggggaatct gggtgaagct caccggacgg ggcaagctcg
     2101 cgttctatcc cgatctggtc aaactcctgg attacgactg ggcgtactcc tcgctcaaag
     2161 cgcggcgcga gctcgggtac acctcgcgct cggtgtgggt gacgctggac gatctgctcc
     2221 acaaccggtt tctcgggacc tacctgcggc cggcgacgga ggggaaatcc gaggcggcgg
     2281 agaagacgcc ctcgtgaggc cttggcgggg aagccgccgt ttgtttatat tgcctccctg
     2341 acatccgtac gccggcgggc ttcgtatcag acgaggcgcc gccgtaccat ctcaagccga
     2401 aggagcaggc catgcgcaag cttgtgctgt ttgttgtcgt ggttgcctcc ctgaccggcg
     2461 ggccctcccg tcccgccgca ggggttattc cctcatggga gagccgcacc gggaagctcg
     2521 ggttcatgcc tctgtccaac gaggtgacaa tgctcaggag cgtcgacgat gccggcggcg
     2581 accgggggtg gacgctgcaa ctggtcttga tcaattcgct taaggtgttc acgaccttca
     2641 agttagagtt caccgccgac tacaatttcg actacacgcc ggggcagaac cgggaccact
     2701 atgtcgagct gtcgttggtc aagccggtga ccgggctggt ctcgctgaac taccagcggg
     2761 tgttgtcgac ctttgaagcc gaggcggtga accagttcgg ggtgcggctg gcgttctgat
     2821 cgggtgcggg ccggccggga gtgttttcgc ctttctgagg gtctcgggcg cgcatatatt
     2881 ggtgcgattc aaaaccggtg agtgtatgac aaaagtatga gtatcagaaa gacgagcaat
     2941 tggtgtctgc tttgggcggc ggccgccgcg gcggccgggt gcggcgacag cccgggcgac
     3001 gacgccggcc gatccggcgg aaacccgggc ggcgcggcga tgcccgtgaa cgtgatcgtc
     3061 gtgcaacccc agcccctgga caacaaaatc ttcacaaccg gcaccctgct ggccaacgaa
     3121 gaggtcgccc tgcgcccgga gatttccggg cgggtggtcg gcctgtatat cgaggagggg
     3181 aaggccgtga aaaaggggga cctgctggtg aaactcaacg accgcgagct ggaggcgcaa
     3241 ttgaggcgca agcagcacga ggaggcgctg gcggctgaag aagagcggcg ggcgaatgcc
     3301 ctcctcgata tcaaggggat cagccaggag gactacgacc gcacgctgaa caagctccgg
     3361 atgattcagg cggagcgcga ggtgatcgag gcgcagctcg cggagacgga gatcctcgcg
     3421 ccgttcgacg ggacggtcgg gctgcgctac atcagcgccg ggggcgtggt gacgccgagc
     3481 acgctggtgg ccacgatgca ggacaccgac cccgtcaagg tggagttctc gattccggag
     3541 aagcacgccg ggaagctcgc ggtgaagacg ccggtgctgg tgcaggtcgg cgacgcgccg
     3601 gagcgccacg aggggacggt gtacgcggtc gaggccaaag tggacccggc gacgcggacg
     3661 ctgaaagcgc gggcgaccgt gccgaacccg gatgggcggc tgatccccgg gtcgttcgcc
     3721 aaagtggaaa ttacgctgga gcatctcccc gacgccctga ttgtgccggc ccaggcgatt
     3781 gtcccgcagc tcggcggcga cctcgtctat gtgtgccgcg agggcgcggc ccgggcgacg
     3841 ccggtgacgg tcggccttcg gggagagcgc accgcccaga tcatccaggg acttgccccc
     3901 ggcgacaccg tcatcaccac cgggctgctg cagttgaacg acgggaagaa ggtggcggcg
     3961 cggcccgagc cggccgaccg cagccggacc ggactatgaa catttcctcc ctgtgcatca
     4021 gccggccggt gctcgccacc gtgctctcga tcctgatcgt gctgttcggg gggatcggct
     4081 ttttcttcct cggcgtgcgg gagtatccga atgtcgaccc gcccatcatc tcggtctcga
     4141 ccgcgtacac cggggccaac gccgacgtga tcgaatcgca gatcaccgag ccgctcgagg
     4201 aggagatcaa cgggatttcc gggatccggt cgctgacgtc gacgagccgc gacgggcgga
     4261 gctcgatccg ggtcgagttc gagctcgggg tggacatgga ggcggcggcc aacgacgtcc
     4321 gggaccgcgt gtcgcgcgcc ctggggagcc tgcccaaaga tgtggacccg ccgatcgtgt
     4381 cgaaggcgga cgccgacgcg acgccgatcc tctcgatgac gctccagagc gaccggcggt
     4441 cgctcctgga actgtcggcg atcgcgaacg acgagtttaa ggagc
//
LOCUS       DAOOKS010000050         4471 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_23456_length_4471_cov_3.912364, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000050 DAOOKS010000000
VERSION     DAOOKS010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4471
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23456_length_4471_cov_3.912364"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..828)
                     /locus_tag="PK112_01460"
     CDS             complement(82..828)
                     /locus_tag="PK112_01460"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake domain-containing protein"
                     /protein_id="HPC10746.1"
                     /translation="MTPPATTSASGKINLWDRLEIVIGDGPEAGRYITRLEDFGQGIL
                     VIGAPEFVGGTDLIRNGAPCRVIFTRDDAVYRFDSQIAVIARREKRIYLLRAPEAIQR
                     VQRRQFVRLDLRRPLTVTVLGQPGSPSLPPHAVHRTVSLNLSGGGMLIEAPFELRPGA
                     RLLADVEFFPAIGIPTPVAAVVRRVDRPAAGGLAGIEFVRADYLAQVFTADELGRLPE
                     SVQQFSRSMQNKLVNYVFQEQVKLRQKGLL"
     gene            complement(844..2028)
                     /locus_tag="PK112_01465"
     CDS             complement(844..2028)
                     /locus_tag="PK112_01465"
                     /inference="COORDINATES: protein motif:HMM:NF021501.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2225 domain-containing protein"
                     /protein_id="HPC10747.1"
                     /translation="MASDSPFFLSKVECPICRTINEFETVRMGAYVEEGRDSDFCPRD
                     IKWRYPKYDGYNPLVFFVATCGNCYYSREYTPAYKEWKKDNAFRTYRLKATKDKHLDQ
                     LATADSIVKRLGKAIDMARFPNQTAILKLHLAAFDEQLFEHYSRLDLGRFYLRIGWVF
                     RSLEGGENPQISRMRGFLYELDTRHDGITDALGSARDRVAEFLESVQSQFEADTLTSE
                     LKAQILPYRERFLAEGAALTERITGVEQRLADLTTLIEEFKTVGVGLGDINSEPTFAG
                     FPTFAEFLYDLKKSWDGIVTSEREALQKAVHFYRQAFTDGRSIAPGNQQIQASYLIAE
                     LSRRTGDFATAREFFNHTIRHGQEFIYRNRHDQTQTALARKILELAIEYGKSSMEAPQ
                     GA"
     gene            complement(2050..2430)
                     /locus_tag="PK112_01470"
     CDS             complement(2050..2430)
                     /locus_tag="PK112_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10748.1"
                     /translation="MPLTNDMWIDLALTATGYLAAGALSMVMYSMFRRKTRPGAAEAP
                     SPAAAPAPAPPLAADRPRDDVRYVGFGERAAAGEPAASLRRNRAEVLRLARGMLRTGA
                     TDAEIKAVLPVSDAELAVLNYERS"
     gene            complement(2449..2778)
                     /locus_tag="PK112_01475"
     CDS             complement(2449..2778)
                     /locus_tag="PK112_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10749.1"
                     /translation="MPPSIETIDKLPPPPPHDRVNETAKANEEQDREAFKRALKRRMA
                     GGDRRADDGPDDPLIVDVDLSKQESPPDQENRDDDPTEPHQAQDRAADADDREEPPAP
                     LHIDLQA"
     gene            complement(2810..3685)
                     /locus_tag="PK112_01480"
     CDS             complement(2810..3685)
                     /locus_tag="PK112_01480"
                     /inference="COORDINATES: protein motif:HMM:NF005413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HPC10750.1"
                     /translation="MVSTAKVEPKKRGCKPRRVSVAETITPEITPKKTRKWDVTARDW
                     AQYSKSRDPEARQRLLAKYIPLVRNVASRMAMGFPRSVELPDLVNTGVIGLIEAFKNF
                     DPGRGVKFETYAVPRIRGAILDELRALDWVPRSTRAKSREIERALVDLENELGRPPEK
                     NELAKHMKISERELHLAIEDVSCTQILSLDEVIYREDDNRQVPRIETIQDSTRATVLG
                     EIERGELRSFLEVAMDRLTEQERLVISLYYHEELTLKEIGAVMVISESRVSQIHTRAV
                     SKLRSMVRERFALTG"
     gene            complement(3693..3929)
                     /locus_tag="PK112_01485"
     CDS             complement(3693..3929)
                     /locus_tag="PK112_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10751.1"
                     /translation="MGEGLRCLGFVPEDAAVRTAVAAQQPLAAAAEHSLAVQALTRMA
                     RSLVRELTPEGADRCGLFTLADDKVNPAEADIRK"
     gene            complement(3969..>4471)
                     /locus_tag="PK112_01490"
     CDS             complement(3969..>4471)
                     /locus_tag="PK112_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10752.1"
                     /translation="KSILAFNLAERLAALGLRVLAVDGDVYGGNLHILANRACAVGIG
                     EFIDRRLSLAECRTPLAERLDLLGVSPRLCDPEAWSAGTVGSMLKRLRAEGAAYDVIV
                     CDHSSGRSRIATLIAHGSEINLLVVVPELTSISDCYGLFKHIRQARADIACRLVINRA
                     ADAEEA"
BASE COUNT          679 a         1410 c         1573 g          809 t
ORIGIN      
        1 tgcgccccac cagggtggag aggtcgaagc gctcgatctc gagggcgtcg agcgcctgat
       61 cgccggcggc ttcccgcgcc gtcataacag ccccttctgg cgcagcttca cctgctcctg
      121 aaagacatag ttgacgagct tgttctgcat gctccgcgag aattgctgca ccgactccgg
      181 cagccggccg agctcgtcgg cggtgaagac ctgcgcgagg tagtcggcgc gcacaaactc
      241 gatgcccgcc aggccgccgg cggcgggccg gtcgacgcgg cggacgacgg cggcgacggg
      301 ggtcgggatg ccgatggcgg ggaaaaactc gacatcggcg agcagccgcg caccgggccg
      361 gagttcgaag ggagcctcga tgagcatgcc gccgccggag aggttgagcg acacggtccg
      421 gtggacagcg tggggcggca gcgacgggct gccgggctgg ccgaggacgg tcaccgtgag
      481 tgggcggcgc aggtcgaggc ggacgaactg ccggcgctgc acccgctgga tcgcctccgg
      541 ggcgcgcagc aggtaaatgc gtttctcgcg ccgggcaatg accgcaatct ggctgtcgaa
      601 tcggtacacg gcgtcgtcgc gggtgaagat cacgcggcag ggagcgccgt tgcggatcaa
      661 gtcggttccg ccgacaaact cgggcgcgcc gatcacgagg atcccctggc cgaagtcctc
      721 caggcgggtg atgtaccgcc ccgcctcggg accgtcgccg atcacgatct ccaggcggtc
      781 ccagaggttt atttttccgg acgcggatgt cgtcgcaggc ggtgtcacgg gccggtcctt
      841 tcctcacgcc ccctggggcg cttccatgct cgactttcca tactcgatcg ccagttcaag
      901 gattttgcgg gccagcgcgg tctgggtctg gtcgtgccgg ttgcggtaga tgaactcctg
      961 gccgtggcgg atcgtgtggt tgaagaactc gcgggcggtg gcgaaatcgc cggtgcggcg
     1021 ggagagctcc gcgatcaggt acgaggcctg gatctgctgg ttgcccggcg cgatgctccg
     1081 gccgtcggtg aacgcctgcc ggtagaaatg gaccgccttc tgcagcgcct cgcgctcgct
     1141 ggtcacgatc ccatcccacg acttcttcag atcgtagagg aactcggcga aggtggggaa
     1201 gccggcgaag gtcggctcgc tgttgatgtc gccgaggccg acgccgacgg tcttgaattc
     1261 ctcgatcagc gtggtcaggt cggccaggcg ctgctcgacg cccgtgatcc gctcggtgag
     1321 ggccgcgcct tcggcgagga accgctcgcg gtacgggaga atctgggctt tcagctcgga
     1381 ggtcagggtg tcggcctcga actgcgactg gaccgactcg aggaactcgg cgacccggtc
     1441 gcgagccgac ccgagggcgt cggtgatgcc gtcgtggcgc gtgtccagct cgtagaggaa
     1501 accccgcatc cgcgagatct gcgggttttc cccgccctcg aggctccgga agacccagcc
     1561 gatccgcagg taaaagcggc cgaggtcgag gcggctgtag tgttcgaaga gctgctcgtc
     1621 gaaagccgcg aggtggagct tgagaatggc cgtctggttg gggaaccggg ccatgtcgat
     1681 ggccttgccg aggcgcttga caatcgagtc ggcggtggcg agctggtcga ggtgtttgtc
     1741 cttggtggct ttgaggcggt aggtgcggaa ggcgttgtcc ttcttccact ccttgtaggc
     1801 gggcgtgtac tcgcgggagt aatagcagtt gccgcaggtg gcgacaaaga agaccagcgg
     1861 gttgtagccg tcgtacttgg ggtagcgcca cttgatgtcg cgggggcaga agtcgctgtc
     1921 gcggccttcc tcgacgtacg cgcccatgcg cacggtttca aattcgttga tcgtgcggca
     1981 gatggggcat tccaccttcg acaggaaaaa cggggaatcg ctggccatga ctcgctcctt
     2041 gctcgtcggt taacttcgtt cgtagttgag gaccgccagt tcggcatcgg agacgggcag
     2101 cacggccttg atctccgcgt cggtggcgcc cgtgcgcagc atgccccggg cgagccgcaa
     2161 cacttcggcc cggttgcggc ggagggatgc ggcgggctcg ccggccgcgg cccgctcgcc
     2221 gaaaccgacg taccggacgt cgtcccgcgg ccgatcggcc gcgagcggcg gcgcaggcgc
     2281 gggggcagcg gcgggcgacg gggcctcggc cgctcccggc cgggtcttcc ggcggaacat
     2341 cgaatacatc accatcgaga gcgccccggc ggcgagatag ccggtcgccg tgagcgccag
     2401 gtcgatccac atgtcattcg tcagcggcat aacttccatc ctaagtcttc aggcctgcag
     2461 gtcgatgtgc agcggggcgg gcggctcttc cctgtcgtcg gcgtccgccg cgcggtcctg
     2521 cgcctggtgt ggttcggtcg ggtcgtcgtc gcggttctcc tgatccggcg ggctctcctg
     2581 tttgctcagg tcgacgtcga cgatgagcgg gtcgtccggc ccgtcgtcgg cgcggcggtc
     2641 gccgccggcc attctccgct tgagcgcccg tttgaacgct tcgcggtcct gctcctcgtt
     2701 ggccttggcc gtctcgttca cgcggtcgtg cggcggcggc gggggcagtt tgtcaatcgt
     2761 ttcaatcgag ggcggcatcg cgcgcctgtc tccttttccc gcgtgcggct cacccggtca
     2821 gggcgaaccg ttcgcggacc atgctccgca gcttgctcac cgcgcgcgtg tggatctgcg
     2881 acacgcgcga ctcggagatc accatgaccg cgccgatctc cttgagcgtg agttcctcgt
     2941 ggtagtaaag cgagatcacg agcctttcct gctcggtgag ccggtccatg gcgacttcga
     3001 ggaacgaccg cagttcgccg cgctcgattt cgccgagcac ggtggcgcgg gtggaatcct
     3061 gtatggtttc gatgcgcggg acctggcggt tgtcgtcctc gcggtagatc acctcgtcga
     3121 gcgagaggat ctgggtgcag gagacgtcct cgatcgccag gtggagttcg cgctcgctga
     3181 ttttcatgtg tttggccagt tcgttcttct ccggcgggcg gccgagttcg ttttcgaggt
     3241 cgaccagggc gcgttcgatc tcgcgcgact tggcgcgggt ggagcgcggc acccagtcca
     3301 gcgcccgcag ctcatcgagg atggcgcccc ggatgcgggg gacggcgtag gtttcgaact
     3361 tgacgccgcg gccggggtcg aaatttttga aggcttcgat caacccgatt actccggtgt
     3421 tgacgagatc cggcagctcc accgagcggg gaaaccccat cgccatgcgc gacgcgacgt
     3481 tgcgcacgag ggggatgtat ttggcgagca accgctgccg ggcttccgga tcccgcgatt
     3541 tcgaatactg agcccagtca cgcgcggtca catcccattt ccgagtcttc ttgggtgtga
     3601 tctcgggcgt gatggtctcg gcgacgctca cgcggcgcgg tttgcagccg cgctttttcg
     3661 gctcgacttt ggcagtgcta accatatgtc tgctatttcc ttatatcggc ctcagccggg
     3721 ttaaccttat cgtccgcaag ggtgaaaagt ccgcaccggt ccgccccttc cggggtgagc
     3781 tcgcggacca gagaacgggc cattcgagta agtgcctgaa cggcaagaga atgttcggcg
     3841 gccgctgcca gcggctgctg ggccgccacc gccgtccgca ccgccgcatc ctccggcaca
     3901 aaacccaggc aacgcagccc ctcgcccaaa aaccgctccg tcaccgctcc aaacttctgg
     3961 tgcaggtact aggcctcctc cgcatccgcc gcccggttga tcaccagtcg gcaggcgatg
     4021 tcggcccggg cctggcggat gtgcttgaac agaccgtagc agtccgagat cgaggtcagc
     4081 tccggcacga ccacgaggag atttatctcc gacccgtggg cgatgagggt ggcgatccgg
     4141 gaccgcccgg agctgtgatc gcacacgatg acatcgtaag cggccccttc ggcgcgcagc
     4201 cgcttgagca tggagcccac cgtcccggcc gaccaggcct cgggatcaca caggcggggc
     4261 gagacgccga gcaggtccag ccgctcggcg agcggggtgc ggcactcggc cagcgacaga
     4321 cgccggtcga tgaattcgcc gataccgacg gcgcaggcgc ggttggccag gatgtgcagg
     4381 ttgccgccat agacgtcgcc gtcgacggca aggacgcgga gaccgagagc cgccagccgt
     4441 tcggccaggt tgaaggcgag gatgctcttg c
//
LOCUS       DAOOKS010000051         4464 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_23503_length_4464_cov_3.498072, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000051 DAOOKS010000000
VERSION     DAOOKS010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4464
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23503_length_4464_cov_3.498072"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1094)
                     /locus_tag="PK112_01495"
     CDS             complement(<1..1094)
                     /locus_tag="PK112_01495"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPC10753.1"
                     /translation="MVERKDAVTMSGSEWSYDQVLRGDPRACAARPWDAVIIGAGPAG
                     SVCARQLAHSGHNVLVIDKERFPRHKVCGDLLVPDALAMLRRVDLHARIAALAHASRT
                     IEVASPSGIRFAVPGEYLALSRKRLDYELCRHAAAAGAHVLAGNAAGVEPAAGDGLVR
                     VHLSGVAEPITARFAAVATGAVVDLAQRIGLVTDPEPSAIALRCYVRSPRRRDDTIIS
                     YDESILPGYGWIVPLGEDHGHPGAFLYNVGCGTMYRHVRDGRHMLKRTLHRFMERFPP
                     AREIMAGAEIVSPIAGAALRCGLRGLATAHCGNVLAIGETIGATYPFTGEGIGKAMET
                     GSLAAETLHEALAADDPSLLGRFSERLRELR"
     gene            1191..2099
                     /gene="rsgA"
                     /locus_tag="PK112_01500"
     CDS             1191..2099
                     /gene="rsgA"
                     /locus_tag="PK112_01500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HPC10754.1"
                     /translation="MTEREKGIVVATRGRLFEVCAADGARVLCEVRRKVKHQTEHETP
                     VAVGDDVVITRVSPGGGAIDEVLPRRTAFVRPAKGRETKKQVLAANLDRLAAVVSIQA
                     PPLRPGLIDRFLIAARLGGLEPLVVINKVDLEPPPGLDDVVAVYRSLGCGVFLVSGLA
                     GTGLGELCEALRGHRTLFAGHSGVGKTTLLNAFVPGLRLRVGQVSPITRRGRHTTTTI
                     EMCELPTGGFVVDSPGLRVLGLWEITPDDLRGMYHEFEPFAGECRYQPCSHTHEPGCA
                     VKKAVAAEKIHALRYENYLAIAASLP"
     gene            2165..3067
                     /locus_tag="PK112_01505"
     CDS             2165..3067
                     /locus_tag="PK112_01505"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HPC10755.1"
                     /translation="MAAKNIEFRVGVIILIGLVVLGASLYWLQGYKLERNAQVIQVLF
                     DDVGTLSVGDRVTVSGVHRGKVDRLDLVDDGVLVKILLYRDVVLHRDARFIIRNMGVM
                     GERFIAITPGKEGAPFDFDSVAVGEYDTGLPEVMGELGEMVIEVRNLVGSFRRNLGSD
                     STLEALNQTVRNLEDVSGSMAEFLDANQSGLNSAIRNFQSAAGRLNELVTRNADQVDS
                     TLATVNRTSARLEEFTYRLDTLAGAARQFADMLNNPDGTAQLLLQDRRLYDDLRSTAD
                     NIDDLVKDIRANPRKYLDLKVEIF"
     gene            3073..>4464
                     /locus_tag="PK112_01510"
     CDS             3073..>4464
                     /locus_tag="PK112_01510"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1925 domain-containing protein"
                     /protein_id="HPC10756.1"
                     /translation="MGTFKLALGIHNHQPIGNFQAVFDEAHRKAYLPFLEAVARSPHI
                     RLSLHQSGILWQWQKRAHPEFFRLVGDLVDRGQVELLTGGFYEPILAAIPERDIRGQI
                     ELLTDYLEEHFETRPAGFWLAERVWEPHLPRPLARAGVRFLAIDDTHFLRAGLDPADL
                     HGPYVTEHEGYALVLLPIRKRLRYLVPFGKPEEIVDCLKRAADRNPSGVAVYADDGEK
                     FGAWPQTFEHCYANGWIQNFFEALEKNRDWLEVVPLGAAARLAPVGRVYLPSGSYAEM
                     DRWALPVAAGLALEQFDKRLAAAGEFDRVEPFVAGGHWRGFLTKYEEANLLHKRMLAV
                     SARLARLEKERPDLIPALAAARDRLYAGQCNCPYWHGVFGGLYLPHIRQAVHAALIAA
                     DRMAAEREGASGVRMERRDYDADGCDDVVVETGAFTAVFRPRRGATLVQLALRRHDFD
                     VTDTLTRRRGLPPP"
BASE COUNT          713 a         1596 c         1453 g          702 t
ORIGIN      
        1 cggagctcgc ggaggcgttc gctgaaccgg ccgaggaggg acggatcatc ggcggcgagc
       61 gcctcgtgca gcgtctccgc ggccagcgac cccgtttcca tcgccttgcc gattccctcc
      121 ccggtaaacg ggtaggtcgc cccgatcgtt tcgccgatcg ccagcacgtt gccgcaatgg
      181 gcggttgcga gcccgcgcag cccgcagcgg agagcggcgc cggcgatggg ggagacgatc
      241 tccgccccgg ccatgatctc gcgcgcggga ggaaaccgct ccataaaccg gtgcagcgtc
      301 cgcttcagca tgtgccgtcc gtcacgcacg tggcggtaca tcgtcccgca cccgacgttg
      361 tacaggaagg cgcccggatg gccgtgatct tcgcccagcg gcacgatcca gccgtagccg
      421 gggaggatgg actcgtcgta cgagatgatc gtgtcatcgc ggcggcgggg cgaacggaca
      481 tagcagcgca gcgcgatcgc gctcggttcc ggatcggtga ccaggccgat gcgctgggcg
      541 aggtcgacca ccgccccggt cgcaaccgcc gcgaaacggg cggtgattgg ttcggcgacg
      601 cccgagagat gcacgcgcac caggccgtcc ccggccgccg gctccacccc ggcggcgttg
      661 cccgccagga cgtgcgcccc cgccgctgcc gcgtggcggc agagctcgta gtcgaggcgc
      721 ttccggctca gcgcgagata ctcgccgggg acggcgaagc ggatccccga ggggctggcg
      781 acctcgatgg tccggctcgc atgggcgagc gccgcgatgc gggcgtggag atccacccgg
      841 cggagcatcg ccagcgcgtc cggcacgagc aggtcgccgc acactttgtg gcggggaaac
      901 cgttctttgt cgatgaccag cacgttgtgg ccggagtgcg ccaactggcg cgcgcagacc
      961 gacccggccg gaccggcccc gatgatgacg gcatcccacg gtcgcgccgc gcaagcacgc
     1021 ggatcccccc gcaggacctg atcgtatgac cattccgaac cggacatcgt gaccgcatcc
     1081 tttcgttcca ccacgataca ctgcgcgggc gcgcgggtca acccggtcgg cggtcgcggg
     1141 acaaaatcgc ttgatacgct ccgcgttcgg gcataccttt acggcgacgt atgacggaac
     1201 gggaaaaggg tatcgtggtg gccacccgcg ggcggctctt tgaggtgtgc gcggcggacg
     1261 gtgcgcgcgt gctctgtgaa gtccgccgca aagtcaagca ccagacggag catgagacgc
     1321 cggtcgcggt cggcgatgac gtcgtgatca cgcgcgtctc tcccggcggc ggggcgattg
     1381 acgaggtact cccccgccgg accgcctttg tccggccggc caaaggccgg gaaaccaaaa
     1441 aacaggtcct ggccgccaat ctcgaccgcc tcgcggcggt cgtgtcgatc caggccccgc
     1501 cgctcaggcc cggcctgatc gaccgcttcc tcatcgccgc ccgtctcggc ggactcgaac
     1561 cgctggtcgt gatcaacaag gtcgatctcg agccgccccc cggactcgat gacgtggtgg
     1621 cggtctaccg gtcgctcggc tgcggggttt ttctcgtctc ggggctcgcc ggaaccggcc
     1681 tgggcgagct gtgcgaggcg ctccgcggcc accggaccct cttcgcggga cattcgggcg
     1741 tggggaagac gacgcttctg aacgcctttg tgccgggcct tcgcctgcgc gtcggccagg
     1801 tctctccgat cacccgccgc ggccggcaca cgacgaccac gatcgaaatg tgcgaactgc
     1861 ccaccggcgg atttgtggtc gactcgcccg ggctgcgcgt cctcgggctg tgggagatca
     1921 ccccggacga cctccgcggc atgtaccacg aattcgaacc gttcgccggc gagtgccgct
     1981 accagccctg ctcccacacc cacgagccgg gctgcgcggt caaaaaggcg gtggcggccg
     2041 agaagatcca cgccctccgg tacgagaatt acctggccat cgcggcctcc ctcccctgag
     2101 ggaccggcgc gataaacgaa tccccctccg gggccgatac agagagagat agcaggaaac
     2161 cggtatggcg gcaaaaaaca tcgaattccg cgtcggcgtg attatcctca tcggcctggt
     2221 cgtcctgggc gcctccctct actggctcca gggctacaaa ctggagcgga acgcccaggt
     2281 gatccaggtg ctcttcgatg acgtcggcac cctctcggtc ggcgaccgcg tcaccgtctc
     2341 cggcgtccac cggggcaagg tcgaccggct tgatctggtc gacgacggtg tgctcgtgaa
     2401 gatcctgctc tatcgcgacg tcgtcctcca ccgcgacgcc cgcttcatta tccgcaacat
     2461 gggcgttatg ggcgagcggt tcatcgcgat cacgccgggg aaagagggcg cgcccttcga
     2521 cttcgattcc gtggcggtcg gcgaatacga taccgggctg ccggaagtca tgggagagct
     2581 gggggagatg gtgatcgagg tgcgcaacct cgtcgggtcg ttccgccgca acctcggctc
     2641 cgactcgacc ctggaggcgc tcaatcagac ggtccgcaat ctggaggacg tctccggatc
     2701 gatggccgaa tttctcgacg ccaaccagag cggcctcaat tcggctatcc gcaacttcca
     2761 gagcgccgcg ggccgcctca atgagctcgt cacccgcaac gccgatcagg tggactcgac
     2821 cctggccacc gtcaaccgca cctccgcgcg cctcgaggag ttcacctacc ggctcgacac
     2881 cctggccggc gcggcccgcc agttcgccga catgctcaac aatcccgacg gcaccgcgca
     2941 gctgctcctg caggaccgcc gcctgtacga cgatctgcgc tcgacggccg acaacatcga
     3001 tgatctggtc aaagacatcc gggcgaatcc gcgcaagtat ctcgatctga aagtggagat
     3061 attctagacg ggatgggcac ctttaagctg gccctcggca tccacaatca ccagccgatc
     3121 ggcaacttcc aggccgtctt cgacgaggcg caccgcaagg cctacctgcc ctttctcgaa
     3181 gccgtcgcgc gcagccccca catccggctc tcgctccacc agtcgggcat cctctggcag
     3241 tggcagaagc gggcccaccc cgagttcttc cgcctcgtcg gcgacctcgt cgaccgcggg
     3301 caggtggaac tgctgaccgg cggcttctac gaacccatcc tcgccgccat ccccgaacgg
     3361 gacatccgcg gacagatcga acttttgacc gactacctcg aggagcattt cgagacccgg
     3421 ccggccggct tctggctggc cgagcgggtc tgggaaccgc acctgccgcg cccgctcgcc
     3481 cgggcgggcg tgcggtttct ggccatcgac gatacccact tccttcgcgc cggtctcgac
     3541 ccggccgatc ttcacggacc ctacgtgacc gagcacgagg ggtacgcgct cgtgctcctg
     3601 ccgatccgca agcgcctccg ctatctcgtg ccgttcggca agcccgagga gatcgtcgac
     3661 tgccttaaac gggcggccga ccgcaacccg agcggggtcg ccgtgtatgc cgatgacggc
     3721 gagaagttcg gcgcctggcc tcagacgttt gagcactgct acgccaacgg ttggatccag
     3781 aactttttcg aggccctgga gaagaaccgc gactggctcg aggtggtgcc gctcggggcg
     3841 gcggcgcggc tggcgccggt cggccgcgtt tacctgccca gcggatcgta cgccgaaatg
     3901 gaccgctggg cgctgccggt ggctgccggg ctggctctcg agcagttcga caagcgcctc
     3961 gccgcggccg gcgaattcga ccgcgtcgag ccgttcgtcg ctggcggcca ctggcgcggc
     4021 tttctcacca agtacgaaga ggccaacctc ctccacaagc gcatgctcgc cgtttccgcc
     4081 cgcctcgccc gtctcgaaaa ggagcgcccc gacctgatcc ccgcgctcgc ggcggcgcgc
     4141 gaccgcctct acgccgggca gtgcaactgc ccctactggc acggtgtgtt cggcgggctc
     4201 tacctgccgc acatccgcca ggcggtccac gccgctctca tcgccgccga ccgcatggcg
     4261 gccgaacggg agggagcctc gggcgtgcgc atggaacggc gggactatga cgccgacggc
     4321 tgcgatgacg tggttgtcga aaccggcgcc ttcaccgccg tcttccgccc gcggcgcggc
     4381 gcgacgctcg tccagctcgc cctgcggcgc cacgatttcg acgtcaccga caccttgacc
     4441 cgccgccgag gcctaccacc tccg
//
LOCUS       DAOOKS010000052         4456 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_23557_length_4456_cov_4.331516, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000052 DAOOKS010000000
VERSION     DAOOKS010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4456
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23557_length_4456_cov_4.331516"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2531
                     /locus_tag="PK112_01515"
     CDS             <1..2531
                     /locus_tag="PK112_01515"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HPC10757.1"
                     /translation="DLRVLLGLVRSLPERSVRRARIVRAAVDGLNAYATSGESARAFR
                     EILARELKKPAAASSLAVTAVGHAHIDTAWLWPVRETIRKCARTFATQLDLLARYPDY
                     VFGASQPQHYAFVKEHYPELYAQVRAAVKAGRWEPQGGMWVEADCNVTGGESLVRQIL
                     HGKNFFRDEFGVEVDNLWLPDVFGYSAALPQLLRKSGIDYFLTQKLSWNQTNEFPHHT
                     FRWQGIDGSEVLTHFPPENTYNSQLHPESLAAAEERFRENDYLDEFLCLFGVGDGGGG
                     PKEEHIEMGRRQADLEGAPRVRFGPAREFFRRLAAERDRLPTWTGELYLELHRGTLTT
                     QAYAKWANRRLEHKLRALEMLAAKLPLDRYPQQELDAVWKTVLLNQFHDIIPGSSIGR
                     VYRVAHTEYREALAGCERLAAAVAAELYPADGNCLTLVNTLHYPYEGAVTLPAGWAGC
                     EIDGGGPTPPCQREPDGVAVHVSIPPYAALTLRRTAETTAPAAPPGQSLVLENDLVRY
                     ELAESGALLRCFDKALGLELVVPEAPGNILTLYEDRPNDWDAWDIDAFYRETPLATAR
                     AAAAEPLPAGDVRRGVRFRYRIGRSHIVQTVRLGRMSRRLDFETAVDWREKHRMLRVA
                     FPVTVRSDYAAFDIQYGYLRRATHRNTSWEQARFEVVGHRYADLSDRDHGVALLNDCK
                     YGYRVENGLLDLNLLRAPNYPDPDADQGEHRFTYSLLPHPGDLAASEVMAEAARLNQG
                     LLAAKGVRATNTRLPVRLSGEGVVLETVKKAEKDAGLILRVAETHGRHSTGRLEIDFP
                     NFTLEETDLMERAVGRRLEAAPAAVLRMAPFEIRTLRLTYDTAQ"
     gene            2515..3567
                     /locus_tag="PK112_01520"
     CDS             2515..3567
                     /locus_tag="PK112_01520"
                     /inference="COORDINATES: protein motif:HMM:NF020678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-N-glycosidase F-related protein"
                     /protein_id="HPC10758.1"
                     /translation="MTQRNRRPTASKTELFTVGVLAALALLNPGAGAPRAFETADART
                     IVVFRDHTIHFAPDDSAKYESGGVTVRDKGRVIAARVELPPFAGPVRITGRLALRPIP
                     KDELEMYDRWDRAGNVRLEVAGGPDVELIKFVTAHGGPTEFEVDLSPLAPLLAGPRVI
                     KGFVDTWVTPGWRMDFSLTYAPDSQALNADWAAGLLYEEAFTRDDPGAAGRAAVIEVP
                     EGMERVLLHYYVSGHCTDGTDADEFVSKDNVISVDGAVVYRYPPWRDDCRRFRAINPY
                     CRRLTDGSWSCDYSRSGWCPGDRAAPLELDLTDHLTAGRHTLRFVIEDIRPKDERGNY
                     GYWRVSGQLLGWRQPR"
     gene            3564..>4456
                     /locus_tag="PK112_01525"
     CDS             3564..>4456
                     /locus_tag="PK112_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008145965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HPC10759.1"
                     /translation="MTFPIHVIPRPAEILPTGGGVRLRAPIPVHFAPNDARISRVIAV
                     GSDAWPGGEALWRPVEADQMKADPPGIHLLMEQGPEPVAESYRLLVGERGVRVNAASP
                     SGLFYGLQTLRQLLWPAETGEREIVLPGMEIRDAPRFGWRGMHLDVSRHFFPPAFIKR
                     YIDLLAFHKLNIFHWHLTDDQGWRIEIGRYPRLTEIGAWRTEPDGRRYGGFYTQAEIR
                     EIVRYAAARQVTVVPEIELPGHATAALAAYPEYSCTGGPFQVSNQWGVFDDVYCAGND
                     AAFTFLEDVLSEVADLFPSPYL"
BASE COUNT          740 a         1482 c         1541 g          693 t
ORIGIN      
        1 tcgacctgcg cgtgctcctc gggctggtga ggagccttcc ggagaggagc gtgcgccgtg
       61 cgcgaatcgt ccgcgcggcg gtcgacgggc tgaatgcgta tgcgacgagc ggcgaaagcg
      121 cccgggcatt ccgggagata ctcgcgcgcg aactgaaaaa accggccgcg gcctcgagcc
      181 tggcggtgac cgcggtcggg cacgcccaca tcgacacggc gtggctctgg ccggtgcgtg
      241 agaccatccg caagtgcgcc cggaccttcg ccacccaact ggacctgctc gcgcggtacc
      301 ccgactacgt gttcggcgcc tcccagccgc agcactatgc cttcgtcaaa gaacactacc
      361 ccgagctgta cgcacaggtc agggcggcgg tgaaagccgg ccgctgggag ccgcaggggg
      421 gaatgtgggt ggaggcggac tgcaacgtga ccggaggcga atccctcgtg cggcagatcc
      481 tccacggcaa gaacttcttc cgcgacgaat tcggcgtcga ggtcgacaac ctctggctgc
      541 ccgacgtctt cggatactcg gcggccctgc cgcaactgct ccgcaagagc ggcatcgact
      601 acttcctcac ccagaagctg tcgtggaacc agacgaatga atttccccac cacacgttcc
      661 ggtggcaggg gatcgacggc agcgaggtgc tgacccattt cccgccggaa aacacgtaca
      721 acagccaact gcaccccgaa tcgctcgccg cggccgagga gcgtttccgg gaaaacgact
      781 atctcgacga attcctctgt ctctttggcg tcggcgacgg cgggggcggc ccgaaagagg
      841 agcacatcga aatgggccgg cggcaggccg atctggaggg agcaccgcgc gtgcggtttg
      901 gccccgcccg cgagtttttc cgccgcctcg ccgccgaacg ggaccggctg ccgacctgga
      961 cgggagagct gtacctggaa ctccaccgcg ggaccttgac gacgcaggcg tatgccaagt
     1021 gggccaatcg ccgcctggag cacaagctcc gggcgctgga gatgctcgcg gccaagctcc
     1081 cgctcgaccg ctacccgcag caggagctgg acgcggtctg gaagacggtg ctcctcaacc
     1141 aattccacga catcatcccc ggctccagca tcggccgcgt ctaccgggtg gcgcacacgg
     1201 agtaccggga ggccctcgcc gggtgcgaac ggctggccgc cgcggtggcc gcggagctgt
     1261 atccggccga cgggaactgc ctcactctgg tcaataccct gcactatccc tacgaggggg
     1321 cggtcacact cccggcgggc tgggcgggct gcgaaatcga tggcggcggg ccgacgcctc
     1381 cgtgccagcg ggaaccggac ggcgtggcgg tgcacgtgag cattcctccg tacgctgcgc
     1441 tgacgctccg ccggacggca gaaacaacag ccccggcggc gccgcccggc cagagtctgg
     1501 tgctggaaaa tgacctggtg cggtacgagt tggcggagag cggtgcgctg ctgcgctgtt
     1561 tcgacaaggc gctcggcctc gaactggtgg tccccgaggc gccggggaat attctcacac
     1621 tgtacgagga tcggccgaac gactgggacg cctgggatat cgatgccttc tatcgcgaga
     1681 cgccgctggc cacggcgcgg gcggccgcgg ccgaaccgct gccggccggg gacgtgcgcc
     1741 ggggggttcg cttccgctac cggatcggcc ggtcgcacat tgtgcagacg gtgcggctgg
     1801 ggcggatgtc gcggcggctc gatttcgaaa cggcggtcga ctggcgcgag aagcaccgga
     1861 tgcttcgcgt ggcgttcccg gtcacggtcc gctcggacta cgccgcgttc gacatccagt
     1921 acggctatct ccggcgcgcc acccaccgca acacctcgtg ggagcaggcc cggtttgaag
     1981 tcgtggggca ccgctacgcc gatctgtccg accgcgacca cggggtggcg ctcctcaacg
     2041 actgcaagta cggctaccgg gtcgagaacg gcctgttgga tctcaacctg ctgcgcgccc
     2101 ccaattaccc cgatccggac gccgaccagg gggaacaccg gttcacctac agcctcctcc
     2161 ctcaccccgg tgatctggcc gcctccgagg tcatggccga ggcggcgcgg ctgaaccagg
     2221 ggcttctggc ggcgaagggc gtgcgcgcga cgaacacccg cctcccggtg cggctctccg
     2281 gcgagggcgt ggtcctggag acagtaaaga aagcggagaa ggacgccggc ctgattctcc
     2341 gcgtggcgga gacccacggc cgacactcga ccgggcggct ggagattgac ttcccgaatt
     2401 tcactctcga agaaaccgat ttgatggagc gggcggtcgg ccgacggctt gaggcggcgc
     2461 cggcggcggt gctccggatg gcgccgtttg agatacgcac tttgaggtta acctatgaca
     2521 cagcgcaata gacgaccaac ggcgagcaag acggagctat tcaccgtggg ggtactcgcc
     2581 gcgctggcgc ttttgaaccc gggggcgggc gctccgcgcg ccttcgaaac ggcggatgcc
     2641 cggaccatcg tcgtcttccg agaccacacg attcatttcg cgcccgacga ctctgcgaag
     2701 tacgagagcg gcggcgtgac cgtccgggac aaggggcgcg tgatcgccgc ccgggtcgag
     2761 ctccctccgt tcgccggccc ggtacggatc accgggcgcc tcgccctcag gccgatcccg
     2821 aaagacgagc tcgagatgta cgaccgctgg gaccgcgccg gaaatgtgcg gctggaggtc
     2881 gccggcggcc cggatgtgga actgatcaag ttcgtgactg cccatggggg gccgaccgaa
     2941 ttcgaggtcg acctgtctcc cctggcgccg ctgctggccg gcccccgcgt gatcaaggga
     3001 ttcgtcgaca cgtgggtcac accgggttgg cgcatggatt tttcgctgac ctacgcgccg
     3061 gattcacagg cgctcaacgc cgactgggcg gccgggctgc tctacgagga ggcgttcacg
     3121 cgcgacgatc cgggagcggc cgggagagca gcggtgatcg aagtgcccga ggggatggag
     3181 cgcgtgctct tgcactacta tgtcagcggc cactgcaccg acggcaccga tgccgacgaa
     3241 ttcgtcagca aggacaacgt catctcggtc gacggcgcgg tggtgtatcg ctacccgccg
     3301 tggcgggatg actgccgccg tttccgggcg atcaacccgt actgccgccg cttgaccgac
     3361 ggttcctggt cgtgcgatta cagccgcagc ggctggtgcc cgggagaccg ggcggcgcct
     3421 ctcgagctgg atctgaccga ccacctcacc gccggccgcc acacgctccg attcgtcatc
     3481 gaggacattc gcccgaagga cgagcggggc aattacggct actggcgggt ctccgggcag
     3541 ctcctgggct ggagacagcc gcggtgactt tcccgataca cgtaatcccg cgcccggccg
     3601 agattctccc gaccggcggc ggggtgcggc tgagagcgcc gatcccggtc catttcgcac
     3661 cgaacgatgc gcggatcagc cgggtcattg ccgtcggcag cgacgcctgg ccgggaggcg
     3721 aggccctgtg gcggccggtg gaggcggacc agatgaaggc agacccaccc ggaattcatc
     3781 tgctcatgga gcaagggccg gagccggtcg ccgagtcata tcggctgctg gtcggcgaac
     3841 gcggagtccg ggtcaacgcc gcctcgccgt ccggtctctt ctacggcctg cagacgctgc
     3901 ggcagcttct ctggccggcg gagacggggg aacgggaaat cgtgctgccg gggatggaaa
     3961 tccgcgacgc cccccggttc ggctggcggg ggatgcacct ggatgtgagc cgccacttct
     4021 tcccgcccgc gttcatcaag aggtacatcg acctgctcgc cttccacaag ctcaacattt
     4081 tccactggca tctgacggac gaccaggggt ggcggatcga aatcgggcgc tacccgcggc
     4141 tgaccgaaat cggggcctgg cggacggagc cggatgggcg gcgctacggc gggttctaca
     4201 cgcaggctga aatccgggag atcgtccgct acgcggccgc gcggcaggtg accgtggtgc
     4261 cggagatcga actgcccggg catgccacgg cggccctggc ggcctacccg gagtattcgt
     4321 gcaccggcgg gccgttccag gtatcgaacc agtggggcgt gttcgatgat gtctactgcg
     4381 cgggcaacga cgccgccttc acatttttgg aggacgttct cagcgaggtc gcggatctgt
     4441 tcccctcccc ctacct
//
LOCUS       DAOOKS010000053         4440 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_23655_length_4440_cov_3.529761, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000053 DAOOKS010000000
VERSION     DAOOKS010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4440
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23655_length_4440_cov_3.529761"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1331
                     /locus_tag="PK112_01530"
     CDS             <1..1331
                     /locus_tag="PK112_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10760.1"
                     /translation="PPTTGIGWEGLRWVGMRDSGLFVGAAGQYREYPHTGAPGNDVRT
                     ILFGPEGRITAGFGDKIFAQYDGDRWMRRIFYRRGDGTMDLLADGRGDIWAASWGGGL
                     FRIRGDSITQYTDSNSTVVGVVAGDPLEYTVVRSLATDGVNIFASLFQAYNKDVVAWA
                     PLDRVHDEAAWGALGVPEGLANEYVTALAYADGELAVGTMARGVYLCRLGSAPYRGTA
                     ITVRHFTDDNSRLISNAIRTVAYAPNGEAWIGTNLGISRWDSGIERLVDVVLPVGVGT
                     DVAAIVFDGRGNAWVGTRGGLVRLDATGGAPDIFTAATSDLVSDEIRDLALDRRSGDL
                     YIATAGGVSVLRTPIENFVETVGDSVVPIPNPFVIRSAADRLRFNLDRPATVSIYTAA
                     GERVAEFPVNEPWDGRNDRGRAVASGAYVYVITDETGASHRGKILLVRKE"
     gene            1328..2251
                     /locus_tag="PK112_01535"
     CDS             1328..2251
                     /locus_tag="PK112_01535"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPC10761.1"
                     /translation="MKAICYHDDDMPEEPVRALTGDSFLASAAFASLWAVRGGRRAVW
                     AAQAGEGIAGVLPGIEFGPAPIKRFMAMPNGLYGRVFAAEGSDPRKTGEVLLEAIAEA
                     GYVKTYLFDFFGCFTSGTGPFAVALQTALLVDIGDPAWEPPDRKLRGEIRKAEREGLA
                     VGRMVWATHGEGFLTLLRGKAHRRGGEARYGADFWQALAHAAQQDERVAWYYASFNNR
                     PVASHIYFVEGDMLIGWQMYYDRKFSPLKANAYLIARACAEARDRGARTLDLGATPEG
                     AGGTEFFKRRWGGREHIYPTLTLRRGIGRWL"
     gene            2242..3489
                     /locus_tag="PK112_01540"
     CDS             2242..3489
                     /locus_tag="PK112_01540"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPC10762.1"
                     /translation="MAVTGAHLRVLVLADARSVHTQRFAAELRRQGCAVLVASLEGSP
                     VDPALGGAEEDPKRTAAPEAAGAPGEPEEGCGASVRLRRRFPIRTVAYAWAAREVERL
                     IERWRPEAISAHYAAGYGYLLSRVRRRPPATLHVWGSDVLVVPHRSRLHLRKVVRGLR
                     AADHIFADSRYLAEEAGRLAGVTNVTVHPWGIERRHLQRRLRVGSIGRPLRIIVPRMH
                     ETVYDNEFVLEALREMIIRGEATVTFPAFGSRYERFRRRAEGMGLRVVSLEAGAARDG
                     TGTEADPRGAVCCYEKRNRDGFLALMAAHDVYVSAARSDSSPATLIEAMALGLAPVAA
                     DIPGVREWLTPESGFAFRPGDAESLRTILARLATGEAGGAECALDGLRRRNREQVERE
                     AIFEDNIAAQLAVMRALCGKAGH"
     gene            3489..>4440
                     /locus_tag="PK112_01545"
     CDS             3489..>4440
                     /locus_tag="PK112_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10763.1"
                     /translation="MRRALLVCYYFPPLSGAGVARPLALFKHLPEFGWECHVLSVKPV
                     AYRAFEPELLDGLPGRHIYRAGSSDPQRLMRLMGIRTVADRVIRKGRALADWYFPDPK
                     IGWVRRATALGRTLLANRDYDAIISTSPPVSAHLVARTLSREFKRPWIADFRDYWTIH
                     RPEQLYANRPRRRRRAQRLLAAIRDEASEVTAVNETVREYVGAGTVISNCYDEALAAG
                     WTLPAGSPFVIGVLGALNEITPVAPLLRLLARLRRERPEVFPRVRVRQVGEVDGQWLE
                     RQIDACGVQEIFDRYGWQPRRETIAILSEASLMYLGVASAE"
BASE COUNT          649 a         1378 c         1712 g          701 t
ORIGIN      
        1 tgccgccgac caccgggatc ggctgggagg ggctgcgctg ggtgggtatg cgcgacagcg
       61 ggctgttcgt gggcgccgcc gggcagtacc gggagtaccc ccacaccggg gcgccgggca
      121 acgacgtccg gacgattctg ttcggaccgg agggacgcat caccgccggg ttcggcgaca
      181 agattttcgc gcagtacgac ggcgaccggt ggatgcgccg gatcttctac cgccgtggcg
      241 acggcaccat ggatcttctg gcggacgggc ggggcgatat ctgggccgca tcgtggggcg
      301 gcgggctgtt ccggatccgc ggcgattcca tcacccagta caccgacagc aactccaccg
      361 tggtcggggt ggtggcgggc gatccgctcg aatacacggt ggtgcggagc ctggccaccg
      421 acggcgtcaa tattttcgcc tcgctcttcc aggcctacaa caaggacgtc gtggcctggg
      481 cgccgctcga ccgcgtgcac gacgaggcgg cctggggagc gctgggcgtg cccgaagggc
      541 tggccaacga gtatgtcacg gcgctcgcgt atgccgacgg cgagctggcg gtcgggacta
      601 tggcccgcgg ggtctacctc tgccggctgg gcagcgcgcc ctaccggggg acggcgatca
      661 ccgtgcggca tttcaccgac gacaactccc ggctcatctc caacgcgata cggacggtgg
      721 cctacgcgcc gaacggggag gcctggatcg ggaccaacct gggaatctcg cgctgggact
      781 cgggcatcga gcggctggtc gatgtcgtgc tgccggtcgg ggtggggacc gacgtggccg
      841 cgatcgtttt cgacgggcgg gggaacgcct gggtgggaac gcgcggcgga ctcgtgcggc
      901 tcgacgccac cgggggggcg cccgacattt tcaccgccgc caccagcgac ctcgtgtccg
      961 acgagatccg ggatctggcg ctggaccggc gcagcggcga cctctacatt gcgacggcag
     1021 gcggggtgtc ggtcctgcgg acgccgatcg agaactttgt cgagacagtc ggggattcgg
     1081 tggtgccgat ccccaatccg ttcgtgattc gctccgccgc cgaccggctg cggttcaacc
     1141 tcgaccgccc ggcgaccgtg agcatctaca cggcggcggg ggaacgggtg gcggaatttc
     1201 cggtcaatga accctgggac ggccgcaacg accgcggccg cgcggtggcc tcgggcgcct
     1261 acgtgtatgt catcaccgat gagacgggag cctcgcaccg gggcaagatt ctgttggtgc
     1321 ggaaggaatg aaagcgatct gctatcatga tgacgacatg ccggaggagc cggtcagggc
     1381 actgaccggc gattcctttc tggcatcggc ggccttcgcc tcgctgtggg cggtccgcgg
     1441 ggggcggcgc gccgtgtggg ccgcccaggc gggggagggg atcgccgggg ttctccccgg
     1501 catcgagttc gggcccgccc cgataaaacg attcatggcc atgcccaacg ggctgtatgg
     1561 gcgagtgttc gcggccgagg ggtccgaccc gcgcaagacc ggagaagtgc tcctggaagc
     1621 gatcgccgag gcggggtatg tcaaaaccta cctgttcgac ttcttcggct gtttcacctc
     1681 cggtacgggg ccgtttgcag tggccctgca aacggcgctg ctggtcgaca tcggcgatcc
     1741 cgcctgggaa ccgcccgacc ggaaactccg gggcgaaatc cgcaaggccg agcgggaggg
     1801 gctggcggtc ggacggatgg tctgggcgac ccacggggag ggattcctga cgctgctgcg
     1861 gggaaaagcg caccgacgcg gcggggaggc ccgctacggt gcggatttct ggcaggccct
     1921 cgcgcacgcc gcgcagcagg atgaacgcgt ggcgtggtac tatgcgagct tcaacaaccg
     1981 gccggtggcg tcgcacatct acttcgtcga gggcgacatg ctcatcgggt ggcagatgta
     2041 ctacgaccgg aagttctcgc cgctgaaggc taatgcgtac ctgatcgccc gtgcctgcgc
     2101 cgaggcgcgc gaccggggcg cgcggacgct cgatctcggc gcgacgccgg agggggcggg
     2161 cggaaccgag ttcttcaagc ggcgctgggg cggacgggaa cacatctacc cgacgctcac
     2221 gctgcggcgc ggaatcgggc ggtggctgtg accggtgcgc acctgcgcgt gctcgtgctg
     2281 gccgatgcgc ggtcggttca cacgcagcga ttcgcggccg agttgcgccg gcaggggtgc
     2341 gcggtgctgg tggcgtcgct ggaaggatcg cccgttgacc ccgccctggg cggcgctgaa
     2401 gaggatccga agcggaccgc tgctccggag gccgcgggtg cgccgggaga accggaggag
     2461 gggtgcggcg cctcggtccg gctgcggcgg cggtttccca ttcggacggt cgcgtatgcc
     2521 tgggcggcgc gtgaggtcga gcggctgatc gagcggtggc ggccggaggc gatcagtgcg
     2581 cactatgccg cggggtatgg ttacctgctc agccgggtgc ggcggcggcc ccccgcgacg
     2641 ctccacgtct gggggtccga tgtcctggtc gtgccgcaca gatcgcggct gcacctgcgc
     2701 aaggtggtgc gggggctgcg cgccgcagac cacattttcg ccgactcccg ctaccttgcg
     2761 gaagaggcgg ggcggctggc cggagtgacc aacgtcacgg ttcacccgtg ggggatcgag
     2821 cggcggcacc tgcagcggcg cctgcgcgtc gggtcgatag ggcggccgct ccgcatcatc
     2881 gtcccccgca tgcatgaaac cgtctacgat aacgagtttg tcctcgaagc gctgcgggaa
     2941 atgatcattc ggggggaggc gacggtgacg tttccggcgt tcgggtcgag gtacgagcgg
     3001 tttcggcggc gcgcggaggg gatggggttg cgcgtcgttt ctctggaggc gggcgcggcg
     3061 cgggacggca cgggaacgga agccgatcct cgcggcgccg tctgctgcta cgagaagagg
     3121 aaccgcgacg ggtttctcgc cctcatggcg gcccatgatg tgtatgtgtc ggcggcgcga
     3181 tccgattcct cgccggcgac gctgatcgag gcgatggcgc tcggtctggc gccggtcgcg
     3241 gccgatatcc cgggcgtgcg ggagtggctg acgcccgaaa gcgggtttgc ctttcggccg
     3301 ggggatgcgg agagcctccg gacgattctc gcgcgcttgg cgaccgggga ggccggcggc
     3361 gcggagtgcg cgctcgacgg cctgcggagg cgcaaccggg agcaggtgga acgggaagcg
     3421 atattcgagg acaacatcgc ggcacagctg gcggtgatgc gcgctctgtg cgggaaagcg
     3481 gggcactgat gcggcgggcg ctgctggtgt gctactattt cccgccgctg agcggcgccg
     3541 gcgtggcgcg gccgctggcc ctgttcaaac atctgccgga gttcggctgg gagtgccacg
     3601 tgctctcggt caagccggtg gcgtaccggg cgttcgaacc ggaattgctg gacgggctgc
     3661 ccggccggca catttaccgg gcgggatcgt ccgatcccca gcggctcatg cgcctgatgg
     3721 ggatacgaac ggtcgccgac cgggtgatcc gcaagggacg ggcgctggcc gactggtatt
     3781 tccccgaccc caagatcggg tgggtccgac gggcgacggc gctgggacgg acactgctgg
     3841 cgaaccggga ctatgacgcg atcatctcga cctcgccgcc ggtctcggca cacctggttg
     3901 cgcgcacgct cagccgggag ttcaagcggc catggatcgc cgatttccgc gattattgga
     3961 cgatccaccg gccggagcag ttgtacgcga accggccgcg ccgccggcgg cgggcgcagc
     4021 ggctcctggc ggcaattcgc gatgaagcaa gcgaagtcac ggcggtgaac gagacggtga
     4081 gggagtatgt cggggccggg accgtgatct ccaactgcta cgatgaagcg ttggcggcgg
     4141 ggtggactct gccggccgga agcccgttcg tcatcggcgt cctcggcgca ctcaacgaga
     4201 tcacgccggt ggccccgctc ctgcggctgc tggcccgcct ccggagggag cggccggagg
     4261 tgtttccccg cgtgagagtg cggcaggtcg gagaggtcga cgggcagtgg ctcgagcgcc
     4321 agatcgatgc ctgcggcgtg caggagattt tcgatcgcta cggctggcaa ccccgacggg
     4381 agacgatcgc gatcctctcc gaggcgtcgc ttatgtacct cggggtggcc tcggcggagg
//
LOCUS       DAOOKS010000054         4355 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_24267_length_4355_cov_3.729535, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000054 DAOOKS010000000
VERSION     DAOOKS010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4355
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24267_length_4355_cov_3.729535"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1512)
                     /locus_tag="PK112_01550"
     CDS             complement(<1..1512)
                     /locus_tag="PK112_01550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPC10764.1"
                     /translation="MQLPRDWPTRLAQAEACLRRGEAEQALEALDGGGDTLPAEPAAK
                     SRLFRLRALARWRRDDALTAEEEARRALAIDPESLDAHYILCRVAARLREYAEAEAAA
                     ERYIEAFRRAFQADGRGDEYTGDYRCLSQVLEIQGQAALARGDRAAATTAFERAIKAD
                     PGHQAPYLSLAEAYRRDGRRDEACAAVERGLRACARTDELRMLAETLVARPTVSACLI
                     VRNEEELLPGCLASIRDWVDEIIVVDTGSTDRTAAIAQEYGAKVFHQAWEGDFAKHRN
                     YSLAQATGDWILVIDADERFSEADVPAIRRYLDDPRARAIAVNILNAAGAHGELTTFL
                     PSVRLFRRELGLRYEGIVHNQLLLDPAEPVVRAAATIEHLGYALAPEKMRAKAERTLT
                     LLERQLREDPDNAFALFNTAQVLLGLGAPADEATAQRIMDAARRATELTDPSAPGERH
                     IHLMATYQRALACYLTGAYDEARQQALRALALKDDYLDPMLLLGHVHLRTGEPE"
     gene            complement(1735..2202)
                     /locus_tag="PK112_01555"
     CDS             complement(1735..2202)
                     /locus_tag="PK112_01555"
                     /inference="COORDINATES: protein motif:HMM:NF014660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HPC10765.1"
                     /translation="MVVQTERFGPIEAPEEKLIRLQKPILGFEGCATFFLVEQDEFRP
                     FLWLQSADRPDLAFIVINPLLVRPDYRIEVHAEEVRDLEITSLRQVETYVIVTVPENP
                     RDISVNLQGPIVINRGNNFAKQLVLVNSSYSVRHRLLPAADEEPVRERETVEV"
     gene            complement(2227..4146)
                     /gene="flgL"
                     /locus_tag="PK112_01560"
     CDS             complement(2227..4146)
                     /gene="flgL"
                     /locus_tag="PK112_01560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HPC10766.1"
                     /translation="MRISTNMLNDRVVFNMQRSLARFLRLETQMSSGRRINQPSDDPV
                     GTQRDLGYRTELAKGEQYRSNIEQAQTWMQSYDTILGDLKDIVSSAKEIAVAMANGVY
                     DDVTRNGAAAEVESLYERILQLATSELEGRQVFSGHRTTTKALTSTATGVVYRGDTGQ
                     IDYQIEASARLTVNLIGSEVFLRQLRPVGAEADLDAGVSGGTLVADLHNGAGIDTAPG
                     TFTITDLNLGLTSTIDVSGAATLNDVIAAINAQLAADGITSLTARPGAEGNNLMLDTS
                     ETGLISAATALARLNSGNGVDLQPGQIRVTDGAGINEVIDFSGAATVGDIIAAFNAHM
                     SAAGYGGVTMGLNAAGTGLAITDAGAPPLGLRIEEVAPDQTTAANLGLAGTVGSLLVG
                     ADLAPTAAFRVAETTGTTATDLGLAGDITGDFAGNDLDPRLQPTTPLASLQHGLGLGE
                     GEIVLRQGERTRTIALDAPGMVTVQDLIDALNGCGLDITAAINAGGRGIQIDNNDSTR
                     SLVVADVGNGRAAKALGVWGGADLMGSMLLLIESLRADDQEGTGRLLQNLDDGIEHLL
                     NQRASVGARDIRLERTHSRLTDLELGFTKLLSEVEDADMTKLVTDLATYENNYQAALM
                     ASARIIQPSLLDFLQ"
     gene            complement(4172..>4355)
                     /locus_tag="PK112_01565"
     CDS             complement(4172..>4355)
                     /locus_tag="PK112_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10767.1"
                     /translation="QENRKASKSIRGDLFGMVNLLKDKITGKEQEKDKPAPIDYRKPS
                     PKRKPGGQTDRSKDES"
BASE COUNT          599 a         1560 c         1411 g          785 t
ORIGIN      
        1 ctccggttcc cccgtgcgca ggtgaacgtg gccgagcagc agcatcggat cgaggtagtc
       61 gtctttcagc gcgagcgccc ggagcgcctg ctggcgggcc tcgtcatagg ccccggtcaa
      121 gtagcaggcg agcgccctct ggtaagtcgc catcaggtga atgtgccgtt cgcccggagc
      181 ggacgggtcg gtcagttccg tcgcccgcct cgccgcgtcc attatccgct gcgccgtcgc
      241 ttcgtccgcc ggcgccccca aacccagaag cacctgcgcg gtgttgaaca gggcgaaagc
      301 gttgtcggga tcctcgcgga gctgtcgctc gagcagcgtg agcgtgcgct cggctttcgc
      361 ccgcatcttc tccggcgcca gggcgtagcc caggtgctcg atggtcgccg ccgcgcgcac
      421 caccggttca gccgggtcca gcaggagctg gttgtgcacg atcccctcgt agcgcaggcc
      481 aagttcccgc cggaaaaggc gcaccgaggg cagaaacgtc gtcaactcgc cgtgcgcacc
      541 agcggcgttc aggatattca cggcgatcgc ccgcgcccgc ggatcgtcca ggtaccggcg
      601 gatcgccggc acgtccgcct cgctgaaccg ctcgtcggcg tcgatcacga ggatccagtc
      661 gccggtcgcc tgcgccagcg aataattccg gtgtttcgcg aaatccccct cccaggcctg
      721 atggaaaacc ttcgccccgt actcctgcgc gatcgcggcc gtccggtcgg tcgatccggt
      781 gtccaccacg atgatctcgt cgacccagtc gcggatcgat gcgaggcagc ccggcagcag
      841 ctcttcttcg tttcggacga tcaggcaggc cgacacggtc ggccgcgcca ccagcgtttc
      901 cgccagcatc cgcaattcat cggtccgggc gcaggccctg agtccccgct ccacggccgc
      961 gcaggcctcg tcccggcggc cgtcccggcg gtacgcttcg gccaggctca ggtagggcgc
     1021 ctgatggccg ggatcggctt tgatcgcgcg ttcgaaagcg gtggtggccg ccgcccggtc
     1081 gccgcgcgcg agcgccgcct gtccctgaat ctcaagcacc tgcgagaggc accggtagtc
     1141 cccggtgtac tcgtcccccc gcccgtcggc ctggaacgcg cggcgaaagg cctcgatgta
     1201 tctttcggcg gccgcctcgg cctccgcgta ctcgcgcagc cgggcggcga cgcggcacaa
     1261 gatgtagtgg gcatcgagcg actccgggtc gatggccagc gcccgccggg cttcttcctc
     1321 ggccgtcagc gcatcgtcgc gccgccaccg ggccagggcg cgcagacgga aaaggcggct
     1381 cttggccgcc ggctcggccg ggagggtgtc ccctccgccg tcgagcgctt cgagcgcctg
     1441 ctccgcctcg ccgcgccgga gacacgcctc ggcctgggct agccgcgtcg gccagtcacg
     1501 ggggagctgc aatggagccg cgggcgcgcc cgccgcgcgg gcttcgcgta tcggccgcat
     1561 cgaggcggcc ttccgacgcg accgcgccgc cccggttcgc cgcacacctg cccccgcggt
     1621 cgcgcgccgc ggacggccgg accgtatgcg tcctgtcgct tttgccatgt ttgcctctct
     1681 ccgcgtgcga gcgccgccgc cggccaaccc gagtgcgccg gggcccgcgc cgccctagac
     1741 ttcgacggtc tcgcgttcgc gcaccggttc ttcgtcggcc gccggcagca gccggtgccg
     1801 cacgctgtag ctcgagttca cgagcaccag ttgcttggcg aaattgttgc cccggttgat
     1861 cacgatcgga ccctgcaggt tcaccgaaat gtcccgcggg ttttccggaa ccgtcacgat
     1921 cacgtacgtc tccacctgcc gcagcgaggt aatctccagg tccctcacct cttcggcgtg
     1981 cacttcgatc cgatagtccg ggcgcaccag gagggggttg atcacgatga aagcgaggtc
     2041 cggacggtcg gccgactgca gccagagaaa cggccggaac tcatcctgct cgaccaggaa
     2101 aaacgtcgca cacccttcga aaccgagaat cggtttctgc agacgtatga gcttttcctc
     2161 gggcgcctcg atcggaccaa agcgttctgt ctgcactacc atagtcccat ttcctctccg
     2221 cttgccttat tgcagaaaat ccaacaggct cggctggata atccgggcac tcgccatgag
     2281 tgcggcttgg taattgttct cgtacgtggc caggtccgtg acgagcttcg tcatgtccgc
     2341 gtcttccacc tccgacagga gcttcgtgaa cccgagctcc aggtcggtca ggcggctgtg
     2401 ggtgcgttcc aggcggatat cgcgcgcccc caccgaggcc cgctggttga gcaggtgctc
     2461 gatcccgtca tccagatttt gcagcaacct ccctgtcccc tcctggtcgt ccgcccgcag
     2521 cgactcgatc agaaggagca tcgagcccat gaggtcggcc ccgccccaga cgcccagcgc
     2581 cttggccgcc cgcccgtttc cgacgtccgc aaccaccagc gagcgcgtcg aatcgttgtt
     2641 gtcgatttga attccccgcc cgccggcgtt gatcgcagcc gtaatatcca ggccgcagcc
     2701 gttgagggcg tcgatcagat cctgcacggt caccatcccc ggcgcatcga gcgcgatcgt
     2761 ccgcgtgcgc tctccctggc ggaggacgat ctccccctcg cccagcccca gtccgtgctg
     2821 gagcgacgcg agcggcgtcg tcggctgcag gcgcgggtcg aggtcgttcc ccgcgaagtc
     2881 gcccgtaatg tcgcccgcca ggccgaggtc ggtcgccgtc gtcccggtcg tctcggccac
     2941 ccggaacgcg gccgtcggcg ccaggtccgc tcccaccagg agcgacccca cggttcccgc
     3001 gaggcccaga ttggctgccg tcgtctggtc gggcgcgact tcctcgatcc gcaggcccag
     3061 cgggggcgcc ccggcatcgg tgatcgcaag ccctgtcccg gccgcgttga ggcccatggt
     3121 cacgccgccg taaccggccg cgctcatgtg ggcgttgaag gcggcgatga tgtcgccgac
     3181 cgtggccgcc ccggagaagt cgatcacctc gttgatcccc gccccgtcgg tcacccggat
     3241 ctggcccggc tggaggtcga ccccgttgcc gctgttcagg cgcgcgagcg ccgtggccgc
     3301 cgaaatcaac ccggtctcgc tcgtgtcgag catcaggttg ttcccctcgg ctccgggccg
     3361 ggcggtcagg ctggtgatcc cgtccgccgc aagctgcgcg ttgatggccg cgatgacgtc
     3421 attgagggtc gctgcccccg acacgtcgat ggttgacgtg aggccgaggt tgaggtccgt
     3481 aatcgtgaat gtcccgggcg ccgtgtcgat gcccgcaccg ttatgcagat cggccacgag
     3541 cgtgccgccg gagacgcccg cgtcgaggtc cgcctcggcg ccgaccggcc gcaactgccg
     3601 gagaaacact tccgacccga tgaggttgac ggtgaggcgg gccgaggcct cgatctggta
     3661 gtcgatttgg ccggtgtcgc cgcggtagac gacgccggtt gcggtcgagg tgagtgcctt
     3721 ggtggtcgtg cggtggcctg aaaacacctg ccgcccctcc agttcgctcg tggccagctg
     3781 gagaatccgc tcgtagaggg actcgacctc ggccgccgcg ccgttccgcg tcacgtcatc
     3841 gtagacgccg ttggccatcg cgaccgcgat ctctttcgcg ctcgagacga tgtccttgag
     3901 gtcgccgagg atcgtgtcgt aggactgcat ccacgtctgc gcctgctcga tgttgctccg
     3961 gtactgctcc cctttggcga gttccgtccg gtagcccagg tcgcgctgcg ttccgacggg
     4021 gtcgtccgag ggctggttga tgcgccgtcc cgacgacatc tgggtttcga gccgcagaaa
     4081 ccgcgcgagc gagcgctgca tgttgaagac cacgcggtcg ttgagcatgt tggtggagat
     4141 tcgcatcgtc accctctcgg ccgacccgcc gtcagctctc gtccttactc cggtccgtct
     4201 gcccgcccgg tttccgtttg ggcgagggtt tccggtaatc gatcggcgcc ggcttgtcct
     4261 tctcctgttc cttgcccgtg atcttgtctt tcagcaggtt gaccatgccg aagagatccc
     4321 cgcggatcga cttcgacgct ttgcggtttt cctgc
//
LOCUS       DAOOKS010000055         4339 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_24387_length_4339_cov_3.419701, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000055 DAOOKS010000000
VERSION     DAOOKS010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4339
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24387_length_4339_cov_3.419701"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..861)
                     /locus_tag="PK112_01570"
     CDS             complement(<1..861)
                     /locus_tag="PK112_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10768.1"
                     /translation="MGIAKFSATVATVLVLLSSGISASGFENSGVGMKARGMGGAFRA
                     IADDWTAAYYNPAGYAYILDNQLGSAWAFVHHRDEFVPNTRWGGTFDRGVFDGRTNYN
                     NHEILTNPSAGILLRLPVAGETVFGLSAYQRFDRNITWKLYEPLRAYSDSANFPDDQF
                     GMNLDVVSFQLTAAKEFAWGERSAALGIGLQLQRADLIYTNVFFRRNPITNSPDEPLY
                     DVMQDYPWDVITQWSRSDGEGWGFGVNLGAMVQLSERTQAGVTFNLPFPITISGTSQS
                     QFYMPDGATMV"
     gene            1472..3817
                     /locus_tag="PK112_01575"
     CDS             1472..3817
                     /locus_tag="PK112_01575"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HPC10769.1"
                     /translation="MRKLIIFILLVIVLVSINWFRRAMAQDLGSLSTEDIARLRRQYE
                     QKSAPAPQPEPGYYESPRIFEEDTASPGPPAAPVAAPSPPAPVRTGFERDRTLEPFED
                     LRPFGMELFENAGETNPPADLVSSDDYVLGPGDNVIVHLWGRVEHEYNLTLDREGKVF
                     IPKVGDLIAWGLTLEQMRQRMKERLAAIYSDFELTASLGKIRSIRIYVTGEVRRPGAY
                     TVSSLTSVLNAVFTAGGPNERGTMRAIAVTRGGAAAAAIDLYDLLLKGHNASDIRLQT
                     GDVVFVPVTGPRVAVRGEIRRPAIYELSGGENVSDLLRLAGGPTAAAYLDRVMLERIS
                     PAGEWLVRDLNLNCGPAQPCDTIALADGDRVTVYSIYDLRTNMVAVGGRVKHPGCYEC
                     TDSTRVSDLIARGQLQPLDVYFERADVFRRYPDRRVEVIPIDLTRALAGDPASDIVLA
                     DRDSLHVYGIDDVRREQFVYIEGEVHKPGKYPLYDGMTVGDLIFLAGSFLRGADAGEA
                     ELARLDSLGNVAILPVSLSDSGGYRRLLCEDDHLFIRRIPEWDANRSVVVSGEVMYPG
                     TYFLEGRHEGLHHLLGRAGGFTANAFPTGIIFQRRSIRDNLQRANVPALLEQSQPIVL
                     DSNGVPTTDSFFSYRDESMDRIIIDMERILATGGSEGDVVMEPGDRVFIPPTPSGISV
                     LGAVGANGTLKYVEGRRVKDYIERAGNFTRRADKKETRLIRAGGEVFAGGDVMGKAVR
                     LGDIIVVPSQIKHDRDWLKTVTTVVTATAGLVTSAYLVSKL"
BASE COUNT          760 a         1465 c         1386 g          728 t
ORIGIN      
        1 caccatggtc gcgccgtccg gcatgtagaa ctggctctgc gaggtgccgc tgatcgtgat
       61 cgggaacggc aggttgaacg tcacccccgc ctgcgtgcgc tcgctcaact gcaccatcgc
      121 cccgaggttg accccgaatc cccacccctc gccgtcgctg cggctccact gggtgatcac
      181 gtcccaggga taatcctgca tcacgtcgta gaggggttca tcgggcgaat tcgtgatcgg
      241 gttccggcgg aaaaagacgt tggtgtaaat gaggtcggcg cgctgcaact gcagaccgat
      301 cccgagggcg gcgctgcgct cgccccaggc aaactccttc gccgccgtca gctggaatga
      361 gacgacgtca aggttcatgc cgaactgatc gtcgggaaaa ttcgcgctgt cgctgtaggc
      421 gcgcaggggc tcgtagagtt tccacgtgat gttccggtcg aaccgctggt aggcgctcag
      481 gccgaacacg gtttcgccgg ccaccgggag gcggaggagg atgccggcgg agggattggt
      541 gaggatctca tggttgttgt agttggtgcg accgtcgaac acaccgcggt cgaaggtgcc
      601 gccccagcgg gtgttcggca cgaactcgtc gcggtggtgc acaaaggccc aggccgatcc
      661 cagctggttg tccaggatgt aggcatagcc ggccgggttg tagtaggcgg cggtccagtc
      721 atccgcaatc gcccggaacg cgccccccat gccccgggct ttcatcccga cgcccgagtt
      781 ttcgaagccg ctggcgctga tcccggagga caacaaaacc agaacggtcg ctaccgtggc
      841 agagaatttc gcaattccca tcctgatctc cgtgtttgat atcatctcaa atgcgtctct
      901 ctcgtggtca gtgatcgaag cacggatggg ggccgacagg aacgagtcgg agagaaacca
      961 ccaggggccg acagtatctc ctccacggct tcggtcaaca aacaggcaac catattaagg
     1021 cggccgcagg gacttgtcaa ggacttccac ctgataatgt aagtctgcgc gcctgattaa
     1081 gcatatgcca tcacgcacgt tagcattcga cacacagacg tgcgacgggg cgccggcgtg
     1141 cagtccatac ggcgcgccgg agaaaattgg acaagttgaa agacgttgcc cgctttgctc
     1201 ttatgaaagc cccgccgcac actccccctc cccgccccca gattgtcgtc tccggctccg
     1261 gaaaaaactt cccgccacag gggacaactt tgcccacgca taacctgctg cggcgggtcg
     1321 cggcggccgc ctgccagcct gaggcgcaac gcgcacaagc gcttgccgca tcgccccgat
     1381 ctccggcaca cggggtgctt ttgcctgcgg caacggccgc cggaggcggt cgtcaggcaa
     1441 aaggaagttg cattacagga aggtatcgtc catgcgcaag ctcatcatct tcattctcct
     1501 ggtgatcgtg ctcgtctcga tcaactggtt ccgccgggca atggcgcagg atcttggctc
     1561 gctcagcacc gaggacatcg cgcgcctgcg ccgccagtac gagcagaagt cagcgccggc
     1621 cccccagccc gagccgggct actacgagag cccccggatc tttgaggagg acacggcgtc
     1681 gccgggcccg ccggcggcgc ccgtcgcggc gccgtcgccg ccggccccgg tgcgcaccgg
     1741 ttttgagcgc gaccggacgc tcgaaccgtt cgaggacctg cgccccttcg gcatggaact
     1801 ctttgagaac gccggcgaga ccaacccgcc ggccgacctc gtgtcgagcg acgactacgt
     1861 gctgggaccg ggcgacaacg tgatcgtcca cctgtggggc cgggtcgaac acgagtacaa
     1921 cctcacgctc gaccgggaag ggaaagtgtt cattccgaaa gtcggcgacc ttatcgcctg
     1981 ggggctcacc ctggagcaaa tgcgccagcg gatgaaagag cgcctcgcgg cgatctactc
     2041 cgatttcgaa ctcaccgcct cgctcgggaa gatccgctcg atccgcatct acgtgaccgg
     2101 ggaagtccgc cgtcccggcg cctacacggt gtcgtcgctc acgtcggtgc tcaacgcggt
     2161 gttcacggcc ggcgggccga acgagcgcgg cacgatgcgc gccattgcgg tcacgcgcgg
     2221 gggcgcggcg gccgccgcca tcgaccttta cgatctgctc ctcaaggggc acaacgcctc
     2281 ggacatccgg ctgcagacgg gcgatgtcgt gtttgtcccg gtgaccgggc cgcgggtggc
     2341 ggtgcggggg gagatccggc ggccggcgat ctatgagttg tcgggcgggg agaacgtatc
     2401 cgacctgctg cggctggccg gggggccgac ggcggcggcg tacctcgacc gcgtcatgct
     2461 cgagcggatc tcgccggccg gcgagtggct cgtacgcgac ctcaatctca actgcgggcc
     2521 ggcccagccg tgcgacacga tcgcgctggc cgacggcgac cgggtcaccg tgtactccat
     2581 ctacgacctg cgcaccaaca tggtggcggt cggcggccgg gtcaaacacc ccggctgcta
     2641 cgaatgcacc gactccacgc gggtctcgga cctgatcgcc cgcggccagc tgcagccgct
     2701 cgacgtctat ttcgagcggg ccgatgtgtt tcgccgctac cccgaccggc gggtcgaggt
     2761 gattccgatc gatctcaccc gggcgctggc cggcgacccg gcctccgaca tcgtgctggc
     2821 cgaccgcgat tcgctccacg tgtacggcat cgacgacgtg cgccgcgagc agttcgtgta
     2881 cattgaaggt gaggtgcata aaccgggcaa gtacccgctc tacgacggga tgacggtcgg
     2941 cgacctgatc ttcctggccg gctcgttcct gcgcggcgcc gacgccggcg aggccgaact
     3001 cgcccgcctc gactcgctcg gcaacgtggc tattctgccg gtgagcctga gcgactccgg
     3061 cggctaccgc cggctgctgt gcgaagacga ccacctcttc atccgccgca ttcccgagtg
     3121 ggacgccaac cggagcgtcg tcgtcagcgg cgaagtgatg tatccgggga cgtacttcct
     3181 ggaggggcgg cacgaggggc tgcaccatct gctcggccgg gccggtgggt tcaccgccaa
     3241 cgctttcccg accggcatca tcttccagcg gcgctcgatc cgcgacaacc tccagcgcgc
     3301 caatgtcccc gcgctcctgg agcagtcgca gcccatcgtg ctcgactcca acggcgtccc
     3361 caccaccgac agctttttca gctaccgcga tgagtcgatg gaccggatca tcatcgacat
     3421 ggagcgcatc ctggccacgg gcggttcgga aggggacgtg gtgatggaac cgggcgaccg
     3481 ggtgttcatc ccgccgactc cctccggcat ctccgtcctc ggcgcggtgg gggccaacgg
     3541 cacgctgaag tacgtggagg gccgcagagt gaaagattac atcgagcggg cgggcaattt
     3601 cacccggcgc gccgacaaga aagagacgcg gctcattcgc gcgggcggcg aagtgttcgc
     3661 cggcggcgac gtcatgggca aagctgtccg gctcggcgac atcatcgtcg tacccagcca
     3721 gatcaagcac gaccgcgact ggctcaagac ggtcaccacg gtggtcacag ccacggccgg
     3781 actggtgacg tcggcctacc tggtgagcaa actctagcgg tccaagattc ctgcatttat
     3841 ttcctttacc ttcctgtagc cgccggcgct gtgacgacag caccggcggc gtttttttgt
     3901 cgggcggaat tgttttcgct agccgaggga aattcctgcc tcgcgccccc gccgggggtg
     3961 gtcgcacgcc gccgcccggg actctcacac gcacgcctta ccgccgtcgc cacaacgagt
     4021 tccgcctccg ctggcggttc ccgcgccatt cggcacaggg cttgcaattc ctgcccgcaa
     4081 aggaaatctg gcagcgacaa ggaatgttgt gccgatggca accactcact cacagcgacc
     4141 cccgcgaccc ggcggcgact ggcagtcggc cggtcccacc gcggcggtcg ccctctctcc
     4201 ccgacacaga cgcgggcggc agcgccgccg gaatatgagg taagcgtatg aacactctcc
     4261 aggacacgcc caccaccgat cgctccgacc tgctcgaggc gatcgcccag agaaccatga
     4321 ccagtccctc gatggtgca
//
LOCUS       DAOOKS010000056         4315 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_24567_length_4315_cov_2.696714, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000056 DAOOKS010000000
VERSION     DAOOKS010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4315
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24567_length_4315_cov_2.696714"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            392..1579
                     /locus_tag="PK112_01580"
     CDS             392..1579
                     /locus_tag="PK112_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010865056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HPC10770.1"
                     /translation="MAAMQMGARAPGQQGKRTRVKYILSIDKLTQLFEAERTVLKEVA
                     RRYAFRTNDYYLGLIDWSDPHDPIRRLVIPAVGELRQWGHLDASREGKVTVRKGVQHK
                     YSSTVLLLVNEVCGSFCRYCFRKRLFMNGNDEAAYDIRPGIEYLRTRPEVNNVLLSGG
                     DPMILSTPQLDGILTALRSIAHIRIIRIGSKMPAFNPYRFLDDDELFAMLRRHSQPDR
                     RIYLVCHFDHPRELTPEAREAVRRAIEAGLICVNQNPIIRGVSDDPEVMAELWNELSY
                     MGIPQYYVFQGRPTAGNEPYEVPLVEAYCSIERAKRKCSGLAKRIKFCMSHESGKVEI
                     IGVDHRHVYLKYHRAKHYRDEQRLLVCHRNDQAYWLDQLKPVEGYRNEYYSDQGLRLY
                     GFS"
     gene            1782..>2641
                     /locus_tag="PK112_01585"
     CDS             1782..>2641
                     /locus_tag="PK112_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10771.1"
                     /translation="MRRGLVSWAALLCAGIIAGRAAAVPPTISYQGRLTDGGAPVPDN
                     TYEIVFRIYAADAGGSPLWLETQEVATADGLFTVLLGSVAPIGEGVFDGSVRYLTMQQ
                     EGAAESAPRIPIVSVPYAMTAGSVEGAAADCSDCDTIFLNAIGPDELRAAVDTALKVT
                     DTGAAARVGLRARLETSTTDTVSAIRGIAYNGGSGRTQAASFDATGEGGGPVHGINVQ
                     ATGNGSSYTTMGVTAQATNDAAGTARGGYFAATNDGFGDHVGAIGQSYSPNTSAAKSI
                     GLWGNARHYG"
     assembly_gap    2642..2651
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2652..4100
                     /locus_tag="PK112_01590"
     CDS             <2652..4100
                     /locus_tag="PK112_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10772.1"
                     /translation="GIGRCVGGMFAAADGANTFGVDASATSANADSTCAVRGYAKNSA
                     TGGTSDAIGGVFIADSAGTAWKWGSIGLAYGGSREATGVYGWAETNATVSGATGVFGQ
                     ASNTGPGDTQGGYFWGHGESGDTYGAKTFGYSTTGSVSGLYAHGSVTDNVYPVYGVYG
                     RVDNYSSGSFNSEAYAGYFENCVYNASNAYGLYGHVYDVQGIYACYGVVGRTAHTGSG
                     SSIAGSFETDSTGTGTAYGVRASGLSKGSAPAYGVYGSVASRGNGNLYAGYFKVSDYG
                     TGLRYGVYASSSVSGYAGYFSGDVRVTGDQVVVGSKSAAVEVEPEDYRLVYSQESPEC
                     WFEDFGEGQLVNGRTHVELDPVFLRTVTVSAQHPMKVFIQLNDPDCNGTAVVRGMTGF
                     DVIELQNGQGSASFTYRVVAKRKGYEDVRLAQMPGQTPEEVEAEAKQAEARLQTEEEQ
                     AAQREIAERDRLAQIRQEERERQEARPFGDRD"
BASE COUNT          825 a         1281 c         1463 g          736 t
ORIGIN      
        1 ctctcggaaa aaccgaggcc catccgcgcg gatgccgcgg tcgcgaagag ccggcagacc
       61 gcccgatggt cggcatcgac caaaatggtc gatgtaacat gctgaaaaac aacagcttgg
      121 cgcctccctg acggccgcgg ggagctcgcg ggaaggcggg gcggggacgt ttttttttgc
      181 cgattaccgt tgctcgggat tcgaatcgat gcgtatattt tttgagaagc caggcctttt
      241 cgattcgagg tgattccggc ggtatcccgc cggttgcggt tcacgcgctg gacccgacca
      301 gatcgatttc gaaccttccg attgcggctg cacgcggcac ggcctggctg acatcctccg
      361 tctggaggca atcatgaaag gagagccgac catggcagcg atgcaaatgg gagcacgggc
      421 cccggggcag caggggaagc gcacacgggt caagtacatc ctctccatcg acaaactcac
      481 gcaattattc gaggccgagc gcaccgtgct caaggaagtg gcgcggcgtt acgccttccg
      541 caccaacgac tactacctcg gcctgatcga ctggtcggat ccgcatgatc ccatccgccg
      601 gttggtcatt ccggccgtcg gggagttgcg acagtggggt catctggacg cctccaggga
      661 ggggaaggtc acggtgcgca agggggttca gcacaagtac agcagcacgg tgcttctcct
      721 cgtcaacgag gtatgcggct ccttctgccg gtactgcttt cgcaaacggc tgttcatgaa
      781 cggaaatgac gaggcggcgt acgacatccg cccggggatc gagtacctcc gcacccgccc
      841 ggaagtcaac aacgtcctgc tgagcggggg tgacccgatg atactttcga cgccgcagtt
      901 ggacgggatt ctcaccgcgc tgcggagcat cgcgcacatc cgcatcattc ggatcgggtc
      961 aaagatgccg gccttcaatc cttaccggtt cctcgacgac gatgagttgt tcgcgatgct
     1021 ccggcgccat tcgcaacctg accgccgcat ctacctcgta tgccatttcg accatccgcg
     1081 cgagctgacg cccgaggcgc gcgaggcggt ccggcgcgct atcgaggccg gcctcatctg
     1141 cgtcaaccag aaccccatca ttcggggggt gtcggatgat ccggaggtga tggcggagtt
     1201 gtggaacgag ttgtcctaca tggggatccc ccagtactac gtattccagg gccgtccgac
     1261 cgccggcaat gagccgtacg aggtcccgct ggtcgaggcg tactgcagca tcgagagggc
     1321 gaagaggaaa tgctcggggc tcgccaagcg catcaaattc tgcatgtcgc acgaatcggg
     1381 caaggtcgaa atcatcgggg tcgaccaccg gcatgtctat ctgaaatatc accgggccaa
     1441 acattaccgc gacgaacagc gcctgctcgt gtgccaccgc aacgaccagg cgtactggct
     1501 cgatcaactg aagccggtcg agggataccg gaacgagtac tactccgatc agggattgcg
     1561 gctgtacggc ttttcgtagg gccacccatc cccatcgcgg atcccgtgaa tgacggctgt
     1621 cgcagggcag ccgttctgct ttggccggag agcgtgcttt ccccttggtg ttcgcgctaa
     1681 aataggtatc tttgtctggc attacagcct gatgaacaac aggttccctg ccgaagctga
     1741 acatatgcag caggatcaag ctctcaacat tgaggtgtag tatgcgcaga ggtctcgtct
     1801 cctgggcggc gctgctgtgc gcgggcatca tcgccggccg cgcggcggcg gttccaccca
     1861 cgatcagcta ccagggccgt ctcaccgacg gcggtgcgcc ggtgccggac aatacgtatg
     1921 aaatcgtctt tcgcatctac gcagccgacg ccggcggcag ccccctgtgg cttgagacac
     1981 aggaagtggc gaccgcggac gggctgttca ccgtgctcct cggttcggtc gcgccgatcg
     2041 gcgagggcgt gttcgacgga tcggtgcggt atctcaccat gcagcaggag ggcgcggccg
     2101 aatcggcgcc gcgcatcccg atagtcagcg tgccgtacgc gatgacggcc gggagcgtgg
     2161 agggagcggc agccgactgc agcgactgcg acacgatctt cctcaacgcg atcgggccgg
     2221 acgaactccg ggccgcggtc gacacggcgc tcaaggtgac cgacaccggc gcggcggcgc
     2281 gggtgggcct gcgggctcgc ctggagacgt ccacgaccga cacggtgtcc gccattcggg
     2341 ggatcgccta caacggcggc tccgggcgaa cgcaggcggc gtcgttcgac gccacgggcg
     2401 aaggcggggg accggtgcac ggaatcaacg tgcaggcgac ggggaacggc agcagctaca
     2461 cgaccatggg tgtgacggcg caggcgacta atgacgccgc cgggacggcg cgcggcgggt
     2521 actttgcggc gacgaacgac ggcttcggcg atcatgtcgg agcgatcggg cagagttaca
     2581 gccccaacac gagcgctgcc aagagcatcg gactatgggg caatgcccgg cactacggga
     2641 tnnnnnnnnn ngggataggc cggtgtgtcg ggggaatgtt cgccgccgcc gacggcgcca
     2701 acacgttcgg ggtggatgcc tcggccacgt cggccaatgc cgactcgacg tgcgctgtcc
     2761 gcggctacgc caagaattcg gcgaccggag ggacgtccga tgcgatcggg ggagtcttca
     2821 tcgccgactc ggccggaacg gcatggaaat gggggtcgat tggcttggcc tacggcggca
     2881 gcagagaagc caccggcgtg tacggctggg ctgagacaaa cgcgactgta tccggggcca
     2941 caggagtctt cgggcaagct tcgaacaccg gtcccgggga cacacaggga ggttacttct
     3001 ggggacacgg cgagtccggc gacacctatg gggcgaagac ttttggctac tctacgacgg
     3061 gcagtgtctc tggcctctac gcacacggat ccgtcaccga caacgtctac cctgtctacg
     3121 gcgtgtatgg ccgggtcgac aattacagct ccggcagctt caactccgag gcctacgccg
     3181 ggtatttcga gaactgtgtc tacaacgcct ccaacgccta cgggctgtac ggccatgtct
     3241 acgacgtcca aggcatctac gcctgttacg gggtcgtcgg ccgcacagcc cacactggct
     3301 ccggctccag cattgccggc agtttcgaga ccgactcgac gggaacagga acagcttacg
     3361 gcgtgcgggc cagcggcctg tccaaaggtt cggcgccggc ctacggcgtg tatggctcgg
     3421 tggccagccg ggggaacggc aatctctacg ccggctactt caaggtctcc gactacggca
     3481 ccggattgcg ctacggggtg tacgcgagtt ccagcgtctc cggatacgcc ggctacttca
     3541 gcggcgacgt ccgggtgacc ggcgaccagg tggtggtcgg gtccaagagc gcggcggtgg
     3601 aggtggaacc ggaggattat cggctggtct actcgcagga gtcgcccgag tgctggttcg
     3661 aggatttcgg cgaggggcaa ctggtcaacg gccggaccca tgtggagctg gatccggtat
     3721 ttctgcggac agtcacggtc agcgcgcagc acccgatgaa agtgttcatc cagttgaacg
     3781 acccggactg caacggcacg gccgtggtgc gggggatgac cgggttcgat gtaatcgaac
     3841 tgcaaaacgg gcaggggagt gcttcgttca cctaccgcgt ggtagccaag cgcaaagggt
     3901 acgaagacgt gcggctggcg cagatgcccg ggcagacgcc ggaggaggtg gaggcggagg
     3961 cgaagcaggc ggaagcccgg ctgcagaccg aggaggaaca ggcggcgcag agggaaattg
     4021 ccgaaaggga tcggctcgcc cagatccggc aggaagaaag ggagcggcag gaagcgcggc
     4081 cgttcgggga tcgcgattag cgttcggcga ttgacgggca ggacggaggc aagacgcggc
     4141 cggcgataac gccggccgcg ttgtcgtcag gagcggctcg ctaagtcgag cgggcaaaga
     4201 agaccgagct attcgtccct tttcgcttgg cggggggctg aaatggggta tacctttaat
     4261 tgtcatcagt tcatctatcc gggacgtgtc ctcatggccg ttacagaagc tgtcg
//
LOCUS       DAOOKS010000057         4311 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_24595_length_4311_cov_3.461701, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000057 DAOOKS010000000
VERSION     DAOOKS010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4311
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24595_length_4311_cov_3.461701"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1393
                     /gene="mutS"
                     /locus_tag="PK112_01595"
     CDS             <1..1393
                     /gene="mutS"
                     /locus_tag="PK112_01595"
                     /inference="COORDINATES: protein motif:HMM:NF003810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HPC10773.1"
                     /translation="LQAAEVADVGHLAGALVDEPPLTHLKGDIFRRGYAPRLDALNDG
                     IRDTRQYIAGLAKTERERTGIGNLKVGFNKVFGYYIEITKSHLGLVPPDYIRKQTLVN
                     AERFITPELKEKEDLIVSAEEKIFKLEEELYLELMEFLNTRIAAIKLAAELAAELDLV
                     SGLAETAVERGYVQPEIYEDARLRITGGRHPVIEAVLPPGSFVANDVTLSPDAGCIHI
                     LTGPNMSGKSTYLRQIGLIVILAQIGSFVPAEKAEIGLADRVFTRVGAVDNLARGQST
                     FLVEMVETANILHNATARSLVLLDEVGRGTSTFDGLSVAWAVVEHINEHIRARTVFAT
                     HYHELTGMAEIYESVHNYQVQVKKWEDRVVFLHHIIPGGCDDSYGIEVARLAGLPRSA
                     IARARQILRLLESGKFTRSELGRGLYKERVQPTLFDQKPSATEEQIRALDLDRMTPLE
                     ALDALRKMKEDLA"
     gene            1390..3264
                     /gene="mutL"
                     /locus_tag="PK112_01600"
     CDS             1390..3264
                     /gene="mutL"
                     /locus_tag="PK112_01600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HPC10774.1"
                     /translation="MTRPTDSPAPQRIRPLPERVVNKIAAGEVIERPAAVVKELVENA
                     LDAGADRVDIVVEKSGARLIKIVDNGCGIAEDQVEIAFARHATSKIAVFDDLNELYTY
                     GFRGEALPSVASVSRLRMVTRPREAAHGTEIIYEGGVLMTKRPSAAAPGTTIEVENLF
                     FNTPARRKFLKAETTEARHISRMAMALAIGRWDVAFSYTLNERKMFAAPAAEAVAERV
                     PTLLSPGRPFLPVAGEAGPVRLSGCIGRPDMIQNNRYGQFFFINRRFIQSATLAHAFL
                     AGYGELIPKGMFPVGALLIEVDPREVDVNVHPTKAEVRLSNERELHEAVRRLVSETLR
                     QDGIVPTFPLRTGDGEKSGGGPPGIRPVPVPPSRQGVIPGVYHARPVSLDQLGEFYRP
                     PEDGPAAADPALPPGLTVDTVTGEVRGDQPADSGPAAPGPSQGFRFIGRFANLYLLIE
                     SAEELYVVDQHTAHERVLYEETLRRLAEQSLSGQSLLFPAQVELSPEQFAVFEESCDL
                     LNRSGFAVAPFGGRTVTIDATPSVLARRQRSPDTMFLHILDDLGSLRRAGQDLTKAMA
                     QSIACRAAVMAGDRLSEHEAVHLLEALLQCRDRYSCPHGRPTFVRITRSDLDRQFGRA
                     "
     gene            3257..4186
                     /gene="miaA"
                     /locus_tag="PK112_01605"
     CDS             3257..4186
                     /gene="miaA"
                     /locus_tag="PK112_01605"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HPC10775.1"
                     /translation="MPEPRAIPILCGPTGAGKTAAAVEAAHGRPIEVVSADSRQMIRG
                     FDIGTAKPTPEERAAVPFHLVDVIDPGERYTAFQFIDDATAAIDDILRRGAIPLVVGG
                     TGFYLRALTTGIVEIAEGDLGIRERLEREMAEQGAEALYAHLTRIDPLEAARLHPNNR
                     VRVIRALEIFEVTGRTRSELAVTGRYRKAAHRFAYLCLAPERPELYARIDARVEQMMA
                     AGLLAEVRGLAARIGPEQVRKANVIGYTELLDHAEGRLALDEAVALVKQNHRRYAKRQ
                     LTWFRHQADCRFFADRPSLMQELRANLAALQGA"
BASE COUNT          755 a         1432 c         1447 g          677 t
ORIGIN      
        1 cctgcaggct gcggaagtgg ccgacgtagg tcacctcgcc ggcgccctgg tcgatgaacc
       61 gccgctcacc cacctgaagg gggacatctt ccgccggggg tacgccccgc gcctcgacgc
      121 cctcaacgac ggaatccgcg acacccggca gtacatcgcc ggcctcgcca aaaccgagcg
      181 cgagcggacg gggatcggca acctcaaggt cgggttcaac aaggtcttcg gatactacat
      241 cgagatcacc aagtcccacc tcggcctggt gccgccggac tacatccgca agcagaccct
      301 ggtgaacgcc gagcggttca tcacgcccga gctgaaggag aaagaggacc tcatcgtctc
      361 ggccgaggag aaaatattca agctcgagga agagctctat ctggagctga tggaattcct
      421 caacacccgc atcgccgcga tcaagctcgc ggccgagctg gccgccgagc tcgatctcgt
      481 ctccgggctg gccgagacgg cggtggagcg ggggtacgtg cagccggaga tttacgagga
      541 cgcccggctg cggatcacag gcggccgcca cccggtgatc gaggccgtgc tgccgcccgg
      601 ctcgttcgtc gccaacgacg tgacgctctc gcccgacgcg gggtgcatcc acatcctcac
      661 cgggccgaac atgtcgggca aatcaaccta ccttcgccag atcggactca tcgtgatcct
      721 cgcccaaatc gggtcgttcg tgccggcgga gaaagcggag atcgggctgg ccgaccgggt
      781 gttcacgcgg gtgggcgccg tcgacaacct cgcccgcggg cagtcgacct tcctggtcga
      841 gatggtggag acggcgaaca tcctccacaa cgccaccgcg cgttcgctcg tgctgctcga
      901 cgaagtgggg cgggggacgt cgacgttcga cggactctcg gtcgcctggg cggtcgtcga
      961 gcacatcaac gagcacatcc gcgcgcgcac cgtgttcgcc acccactacc acgaactgac
     1021 cgggatggcg gaaatctacg agtccgtcca caactaccag gtgcaggtga agaagtggga
     1081 agaccgggtg gtattcctcc accacattat ccccggcggg tgcgatgact cctacgggat
     1141 cgaggtggcc cggctggccg ggctgccccg gtcggcgatc gcccgcgccc gccagattct
     1201 ccgtctgctc gaatcgggaa aattcacccg cagcgagctg ggacgagggc tgtataagga
     1261 gcgcgtgcag ccgacgctct tcgatcagaa gccgtcggca accgaagaac aaatccgggc
     1321 cctcgacctt gaccgcatga cgccgctgga ggcgctcgac gcgctgcgca aaatgaaaga
     1381 ggatctcgca tgacccggcc gaccgattcc ccggcgccgc agcggatacg ccccctgccc
     1441 gaacgggtgg tcaacaagat tgcggcgggc gaagtgatcg agcggccggc tgccgtggtc
     1501 aaggaactgg tcgaaaatgc gctcgacgcg ggcgccgacc gggtcgacat cgtggtcgaa
     1561 aaatccgggg cccggctgat caagattgtc gacaacggct gcggcattgc ggaagaccag
     1621 gtcgaaatcg cattcgcgcg ccacgcgacc tcgaagatcg ccgtgtttga cgatctcaac
     1681 gagctgtaca cctacggatt tcgcggcgag gcgctgccct cggtggcctc cgtctcgcgg
     1741 ctgcgcatgg tgacgcgccc gcgcgaggcg gcgcacggaa ccgagatcat ctacgaaggg
     1801 ggggtgctga tgaccaagcg gccgtctgcg gcggcgccgg gcaccacgat tgaggtcgag
     1861 aacctgtttt tcaatacccc ggcgcggcgg aaattcctca aagccgaaac gaccgaggcc
     1921 cggcatatct cgcgtatggc catggcgctg gcgatcgggc gctgggatgt cgcgttctcg
     1981 tacacgctca acgagcggaa gatgttcgcg gcgccggcgg ccgaggccgt ggccgaacgc
     2041 gtcccgaccc tcttgagtcc gggccggcca tttctgccgg ttgccgggga agccgggccg
     2101 gtgcgcctga gcggctgcat cgggcgcccc gacatgattc agaacaaccg ctacgggcag
     2161 tttttcttca tcaatcgccg gttcattcaa tcggcgacgc tggcgcacgc attcctggct
     2221 ggctacggcg agttgattcc caaaggcatg ttccctgtcg gcgcgctcct catcgaagtc
     2281 gacccgcgcg aggtcgacgt caacgtgcac ccgaccaagg cggaagtgcg gctgtcgaac
     2341 gagcgggaac tccacgaggc cgtccgccgc ctggtcagcg agacgctgcg gcaggacggg
     2401 atcgtcccga ctttcccgtt gcgtacgggc gacggggaga aaagcggcgg cgggccgccg
     2461 ggtatccgtc ccgtgcccgt gcctccctcc cgccagggcg taatccccgg cgtctaccac
     2521 gcgcggccgg tcagcctcga ccaactcgga gagttctacc gcccgccgga agacggcccg
     2581 gccgcggcgg atccggcgct gccgccgggg ctgacggtgg atacggtgac cggcgaggtg
     2641 cgcggcgatc agccggcgga ctccggcccg gcggctccgg gtccctctca gggtttccgg
     2701 ttcatcggcc ggttcgccaa tctctacctg ctgatcgaaa gcgccgagga gctctatgtc
     2761 gtggaccagc acaccgcgca cgagcgagtg ctctacgagg agactctgcg gcggctggcc
     2821 gagcagtcgc tgagcgggca gagcctcctg tttccggccc aggtggagct gagccccgag
     2881 caattcgccg tgtttgagga atcatgcgat ctgctcaacc ggtcgggatt cgccgtggcg
     2941 cccttcggcg ggcggacggt gacgatcgac gcgacgccct cggtgctcgc ccgccggcag
     3001 aggtcgcccg acacgatgtt cctgcacatt ctcgatgacc tcggatcact gcgccgggcc
     3061 gggcaggatc tcaccaaggc gatggcgcaa tcgatcgcct gccgggcggc ggtgatggcg
     3121 ggcgaccgcc tgagcgagca cgaggcggtc cacctcctcg aggccctcct gcagtgccgg
     3181 gaccgctact cctgcccgca cgggcgcccg actttcgtgc gcattacgcg cagcgacctc
     3241 gacaggcaat tcggccgtgc ctgagccgcg cgccataccg atcctctgcg ggccgaccgg
     3301 cgccggcaag acggcggcgg ccgtcgaagc cgcgcacggg cggcccatcg aagtcgtctc
     3361 cgccgattcc cgccagatga tccggggatt cgatatcggg acggccaaac cgacgcccga
     3421 ggagcgcgcg gctgtgccct tccacctcgt cgatgttatc gatcccggag aacggtacac
     3481 cgcttttcag tttatcgatg atgcgacggc ggcgatcgac gacattctcc gccgcggagc
     3541 catacccctg gtggtcgggg gtacgggatt ctatctgcgg gcgctcacga ccggaattgt
     3601 ggagatcgcc gagggggatc tggggatccg ggagcgactg gagcgggaga tggcggagca
     3661 gggcgcggag gcactctacg cccacctcac gcggatcgat ccgctggagg cggcccggct
     3721 ccacccgaac aaccgcgtgc gtgtgatccg ggcgctggag atattcgaag tgaccggccg
     3781 cacacgctcg gaactggccg tgaccggccg ataccgcaag gccgcgcatc gctttgcata
     3841 cctctgcctg gccccggagc gcccggaact gtacgcccgc atcgatgcgc gggtggagca
     3901 gatgatggcg gcggggctgc tcgcggaagt gcgcggcctg gccgcccgga tcggtccgga
     3961 acaggtgcgg aaggccaacg tgatcggcta caccgagctg ctcgatcacg cggaggggcg
     4021 cctggcgctg gacgaggcgg tggccctggt caaacagaac caccgccgct acgccaaacg
     4081 ccagttgaca tggttccgcc accaggctga ttgccgattt ttcgccgatc gaccgtcact
     4141 tatgcaggag ctccgcgcca atttggcggc gctgcagggc gcgtgacggc ggggcctgtg
     4201 cactttttgc gcagtacgag aaaatcaccg ggccggcttg ttgaaaattg acacgaggtt
     4261 tttacacccg tactttggcc aggttgacac aatgaccgcc cctttaaagg g
//
LOCUS       DAOOKS010000058         4299 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_24687_length_4299_cov_3.222432, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000058 DAOOKS010000000
VERSION     DAOOKS010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4299
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24687_length_4299_cov_3.222432"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1974
                     /locus_tag="PK112_01610"
     CDS             <1..1974
                     /locus_tag="PK112_01610"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HPC10776.1"
                     /translation="VFGSPFTWFSDSRTLLVRFLPDDRGEPPVKPRVPEGPNVQENLG
                     AKAPARTYQDLLANPCDEQLFDYYGAAQTALVTVDGAVTPVGRTGVLVNTSPSPDGRY
                     ILAETIHRPYSYTVPAERFPRTIEVWDRAGATVYRVADLPLADRVPITFGSAPTGPRD
                     VDWRADADAALFWVEALDGGDAAVVTDERDRLFLLPAPFTGEPAALLTLGFRYSGVQW
                     GNDSLALVNETWWPTRTVRTWRLRPGRPEARAELIVDRSWEDRYQDPGAPMLDRSGRG
                     TYVLATADTGTVIFRTGAGASPEGNQPFLDAFNVDTKETTRLFRSAAPYYEEPVDFLD
                     IDRRLVLTRRESVDQPPNYFRRDLRHDDLMQITFFPHPTPQLRDVKKELVTYTRADGV
                     QLSGKLYLPPRYDPKRDGPLPMLMWAYPEEFKSAGAAGQVTDSPYRFVRASWSSPLLW
                     LALGYAVLDDPSMPIVGEGDAEPNDTYVEQLVASARAAVDEMVRRGVADRDRIAVGGH
                     SYGAFMTANLLAHSDLFAAGIARSGAYNRTLTPFGFQSEERTFWEAPQTYFTMSPFMQ
                     ADRINEPLLLIHGEADNNPGTYPMQSERFYAALKGQGATVRLVMLPHESHGYQARESV
                     LHVLWEMTQWLDTYVKNRRLAEPGAVKSMSGAE"
     gene            complement(2065..3222)
                     /locus_tag="PK112_01615"
     CDS             complement(2065..3222)
                     /locus_tag="PK112_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly PilX N-terminal domain-containing
                     protein"
                     /protein_id="HPC10777.1"
                     /translation="MDKPFTTERGSALLIALLLTGMLTLVAFIAMDNSNTDIALSYNV
                     MNSDRAFYAAEAGVKRSLVALNADPSWNEGFADVPIGAGSFSVAVEDSSVTPALADTI
                     IVTSTGLVRDAQSTIEMTLVPVRFNPFLKALFGDNSVDIKNSMESDSYNSDSGAYAGT
                     RTWHDGDVGSNGTITIANGAIVGGGVATSQPGGLSINAGATVYDDTTSTAPRQEVPDV
                     PQTEFDWAAAHNQNLSGISGSFTYNPSTKAFETSGSVTLSSGVYYFSSIILKNSASME
                     VAPGATVMIYVTGDIEMKNSAAMNTAGAPKDLQIYSQGDIVLKNSGDIHAVFYSAGGE
                     ADLRNSGQFFGAVVARDIVVHNSAKFHYDRSLGEIEKNWDAHMKVVAWREL"
     gene            complement(3327..>4299)
                     /locus_tag="PK112_01620"
     CDS             complement(3327..>4299)
                     /locus_tag="PK112_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10778.1"
                     /translation="DNDDWRSGYADFGFGQGTFSVNLFDSLTTPSLADTVVIRSRSDV
                     REAQAEVEMWTVPVYINPFVWAMFADSAITMDQTTCTDSYNSDSGAYAATVLQDEGSI
                     GTNGRISTSKTVTIGGDAKTAVGGSITLGAGSQVLGDTSTTQDSVNIDIIPQSEYDWA
                     RTNSLAPLGLSGTGWSYNNGTRKLTMGSYSSVVLQSGVYYFSEIVMGQGSTISLAPGA
                     QVRIYMSGNIDFNQNGRVNASGAPQNLQIYSQGNLFQLDQGNIFCGTFFGPNAHIQYD
                     QTTQVYGALVGNSIKLDKGACFHYDRNLAKIRHGTTGEMIQVAWKEL"
BASE COUNT          694 a         1414 c         1406 g          785 t
ORIGIN      
        1 gtgttcggca gcccgttcac atggttctcg gacagccgga cgctgcttgt tcggttcctc
       61 cccgacgatc ggggagagcc gcccgtcaaa ccgcgcgttc ctgagggccc gaacgtccag
      121 gagaatctcg gggcgaaagc tcccgcccgc acttaccagg accttctggc caacccctgc
      181 gacgagcaat tgttcgacta ctacggggcc gcccagacgg cgctcgtgac cgtcgatggc
      241 gcggtgacgc cggtcggtcg gaccggagtc ctcgtaaaca cgtctccttc gcccgacggc
      301 cgctacattc tggcggagac gattcatcgc ccctactcgt acacggtgcc cgccgagcgt
      361 ttcccgcgca cgatcgaggt gtgggaccgc gccggcgcga ccgtctaccg ggtcgccgac
      421 ctcccgctgg ccgaccgggt gccgatcacg ttcggctcgg ccccgaccgg cccacgcgac
      481 gtcgactggc gcgcggatgc cgacgcggcg ctcttttggg tggaggcgct cgacgggggg
      541 gatgccgccg tcgtcaccga cgagcgcgac cgcctctttc tgcttccggc cccgttcacc
      601 ggcgagccgg cggcgctgtt gacgctgggt ttccgttact ccggtgtcca gtggggcaat
      661 gacagcttgg cgttggtgaa cgagacctgg tggccgaccc gcaccgtccg gacctggcgc
      721 ctccgtcccg gccggccgga ggcccgcgcc gagctgatcg tcgaccggtc gtgggaggac
      781 cgttaccagg acccgggcgc accgatgctg gaccggagcg ggcgcggcac gtatgtcctg
      841 gcgacggccg acaccgggac ggtgatcttt cgcaccgggg ccggcgcctc gccggagggc
      901 aaccagccct tcctcgatgc cttcaatgtg gacacgaagg agaccacccg tctgtttcgt
      961 tccgcggccc cgtactatga agagccggtg gatttcctcg atatcgaccg ccgcctggtg
     1021 ctcacccgcc gggaatcggt tgaccagccg ccgaactatt tccgccgcga tcttcgccac
     1081 gacgatctca tgcagatcac gttcttcccc cacccgaccc cgcagttgag agacgtcaag
     1141 aaggagctgg tcacctatac gcgggctgac ggcgtgcagc tcagcggcaa gctctatctg
     1201 ccgccgcggt atgacccgaa gcgggacggc ccgctgccga tgctcatgtg ggcctacccg
     1261 gaggagttca agtcggcggg cgccgcgggc caggtgaccg actcccccta ccgctttgtc
     1321 cgcgcctcct ggtcgtctcc cctgctctgg ctggcgctcg gatacgccgt tctcgacgat
     1381 ccctcgatgc cgattgtcgg ggagggagat gcggagccga acgacacgta cgtggagcag
     1441 ttggtggcga gcgcccgggc ggcggtcgac gagatggtcc gccggggcgt ggccgaccgc
     1501 gaccgcatcg ctgtcggcgg gcattcctac ggcgcgttca tgaccgccaa cctgctcgcc
     1561 cactccgacc tgttcgccgc cgggatcgcc cgcagcggcg cctacaaccg caccctcacc
     1621 cccttcgggt tccagtcgga ggagcgcacc ttctgggagg ccccgcagac atatttcacg
     1681 atgtccccct tcatgcaagc cgaccggatc aacgaaccgc tgctactgat tcacggcgag
     1741 gccgacaaca accccggcac gtaccccatg cagagcgagc ggttctacgc cgctctgaaa
     1801 gggcaggggg cgacagtccg cctcgtgatg ctcccgcacg aaagccacgg ctaccaggcg
     1861 cgggaatcgg tcctccacgt gctgtgggag atgacgcaat ggctggacac ctacgtgaag
     1921 aaccggcggc tggcggagcc cggcgcggtc aagagcatgt ccggcgccga gtagcccgga
     1981 ggcgggccgg acgatcaaag aaagcggctg ccgtcgccat cgaacggcag ccgctgtagt
     2041 tcgcgcggac ctgggccgcg ggcgctagag ttcgcgccag gcgacgactt tcatgtgggc
     2101 gtcccaattc ttctcgattt cgcccagcga gcggtcgtag tggaacttgg cgctgttgtg
     2161 gacgacgatg tcgcgcgcga ccaccgcccc gaagaactgg cccgaattac gaaggtcggc
     2221 ctcgccgccc gcggaataga acacggcatg gatgtcgccg ctgttcttga gcacgatgtc
     2281 cccctgcgag tagatctgca ggtctttggg agcaccggca gtgttcatcg ccgccgagtt
     2341 tttcatctcg atgtcccccg tcacgtagat catgactgtc gcccccggtg cgacctccat
     2401 cgaggcgctg tttttcagga tgatactgct gaaatagtac acgccgctgc tcagggtcac
     2461 actgccggac gtctcgaagg ctttcgtcga ggggttgtag gtgaagctcc cggaaatacc
     2521 gctgagattc tggttgtggg cggcggccca gtcaaattcc gtctgcggca cgtcgggcac
     2581 ctcctgccgc ggggccgtgc tggtcgtgtc gtcgtagacg gtcgcgccgg cgttgatgct
     2641 cagcccgccg ggctgcgagg tcgccacgcc gccgccgaca atcgccccgt tcgcgatcgt
     2701 gatcgttccg ttcgagccga catccccgtc gtgccacgtc cgcgtgccgg cgtaggcgcc
     2761 ggaatcggag ttgtacgaat cggattccat gctgttcttg atgtccaccg agttgtcgcc
     2821 gaaaagggct ttgaggaacg gattgaaccg gaccggcaca agcgtcatct cgatcgtcga
     2881 ttgggcatcg cggaccaggc cggtcgaagt cacgataatg gtgtcggcca gggccggagt
     2941 gaccgagctg tcctcgaccg ccacgctgaa actgccggcg ccgatcggga cgtcggcgaa
     3001 gccttcgttc caggagggat ccgcgttgag cgcgacgagc gaccgcttga ccccggcctc
     3061 ggcggcatag aaagcgcggt cgctgttcat cacgttgtag gacagggcta tatcggtatt
     3121 ggagttgtcc atggcgatga aggccaccag tgtgagcatc cctgtcagca gcagggcgat
     3181 cagcagggcg ctcccccgtt cagtggtgaa aggtttgtcc atcggtacgt actccccaga
     3241 ttgaaaggtg aaggcggggc ggccgctgtc atggccgccc cgacccaccg tgttctgtgt
     3301 ctcggtccgg cggacggaag gcaccgtcag agctccttcc acgccacctg gatcatctcg
     3361 ccggtcgtgc cgtggcggat cttggcgagg ttgcggtcgt agtggaaaca ggcgccctta
     3421 tcgagcttga tgctgttgcc caccagggcg ccgtaaacct gcgtcgtctg gtcgtactgg
     3481 atgtgtgcgt tggggccgaa aaacgtaccg cagaagatgt tcccctggtc gagctggaag
     3541 aggttgccct gcgagtaaat ctggaggttc tgcggggcgc cgctcgcgtt cacgcgcccg
     3601 ttttggttga agtcgatgtt gccgctcatg tagattctga cctgggctcc cggcgcaagg
     3661 ctgatggtcg acccttgccc catcacgatc tccgagaagt agtagacgcc gctctggaga
     3721 acgacgctgg agtacgaccc catggtcagt ttgcgggtgc cgttgttgta gctccagccg
     3781 gtcccggaga gacccagcgg ggccaggctg ttggtgcgcg cccagtcgta ttccgactgc
     3841 gggatgatgt cgatgttgac cgaatcctgc gtcgtcgagg tgtcgcccag cacctggctc
     3901 ccggcaccga gggtaatgct gccgccgacc gcggtcttgg catccccccc gatcgtgacc
     3961 gtcttggaag tcgaaatgcg cccgttggtg ccgatgctcc cctcgtcctg cagcacggtc
     4021 gccgcgtacg cgcccgagtc ggagttgtag gaatcggtgc aggtcgtctg atccatagtg
     4081 attgcggaat cggcgaacat cgcccagacg aacgggttga tgtacaccgg cacagtccac
     4141 atctcgacct cggcctgcgc ctcccgcacg tcgctgcgcg agcgaatgac cacggtgtcg
     4201 gcaaggctgg gcgtggtcag ggagtcaaac aggttcaccg agaacgtccc ctgcccgaag
     4261 ccgaaatcgg cgtacccgga ccgccagtcg tcgttatcg
//
LOCUS       DAOOKS010000059         4286 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_24785_length_4286_cov_4.362798, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000059 DAOOKS010000000
VERSION     DAOOKS010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4286
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24785_length_4286_cov_4.362798"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /locus_tag="PK112_01625"
     CDS             <1..817
                     /locus_tag="PK112_01625"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HPC10779.1"
                     /translation="EGFIAGLHKSPYHGFSVEFAEHRQYMPGDNIRAIDWKVYAKSDR
                     FYIKQYEEETNLKAYLLLDCSASMAYHSGERIRKLDYAGLLCGALSYLMLRQRDAVGL
                     VTYDRKIRRYIPPRSRSGHLHVLLNEIAAQAPSDTTDISSTLHEMADRIKRRGLVIIL
                     SDLIDEADKLISGLKHFRYNNHEVIVFHILDPRERDFAFPREAVFKDMETGEELTTLP
                     YQIKKDYAKAVAAFSSEIASACRQAAIDYHPIDTAMPFDKALYAFLSKRERLY"
     gene            973..1308
                     /locus_tag="PK112_01630"
     CDS             973..1308
                     /locus_tag="PK112_01630"
                     /inference="COORDINATES: protein motif:HMM:NF025160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HPC10780.1"
                     /translation="MLILAAVLIGHLVEEAATGFRRKFPLGEMPMWVFVMGNVAVYGF
                     CAATFTLVLLARPAGTLMAWVLAVAMALNGIVHIGVMIYRKEYFPGGITAGLVLAAAA
                     GLMAALARG"
     gene            complement(1447..2487)
                     /locus_tag="PK112_01635"
     CDS             complement(1447..2487)
                     /locus_tag="PK112_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10781.1"
                     /translation="MNPLPDPNFLPAPLWLVTVLHLLTLSLHFAAMNFLVGGLLIVVF
                     GRLADKWRHPVVAQFVKLFPTAMAATVTLGVAPLLFVQLVYPVPVYSAAIVTAWFFLG
                     IVGAVMVAYYLLYAAAFGAPGSPRRPAFLTAALLLLAYVAFAYSNLFSIAERPGLAAV
                     LYAGDPSGLRVNPHLGQWLWRWLHMLLGAVTIGGFFIGLLGRRHEDAFALGRNFFLWG
                     MVGAMAVGLVYLLSLADILKPFMHTPAIWWLSASIILSLGSLHFFFTRKFFGAGAMLF
                     VSLLGMVVVRHNVRLLQLEGHFDPAAWPIRPQWDVFLVFLVCFVLALGLVWYMLRLFF
                     ARPSPGASARDA"
     gene            complement(2484..4025)
                     /locus_tag="PK112_01640"
     CDS             complement(2484..4025)
                     /locus_tag="PK112_01640"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HPC10782.1"
                     /translation="MNYPFWDTTLGYGVLMAVIAVVHVFVSHFAIGGGLYLVVAERAA
                     RRRGDDDHLGFLRRLSRFFVLTTVVFGALTGVGIWFIIGLLNPAATAVLIRNFVWGWA
                     AEWCFFIIEILAALLYYYGWDCLTPRRHLIIGWIYFGAAWLSLVIINGIVTFMLTPGD
                     WLATGRFWDGFFNPTYWSSLVLRTGICIMLAGLCALAVAARLPAPGARVRLVRYNAVW
                     GLVGLAVVIPSLFWYVGDIPADLMAAARQTMNIPFAALDLLYLLTAAIAALLAILGLL
                     APRAWRTEVAAVALALGLVWFGSYEWFREALRKPYVITGYMYASGVEGARAADYRADG
                     MLAHMAYRTGDEGADLFRRACRSCHSLENYNALKPFFDGADEEFITAMVQGIGAMRGN
                     MPPWTGTPEEARLLAAHLAARTDRRPFAEVCGLSGVDLGRRVYEVRCGSCHVLGGFND
                     KTQSLAGLSEEEYRTLLDMSDDLGEEMPPFTGGEPERVALIQFLQTLSAPAGTPAAAT
                     RGGAR"
BASE COUNT          875 a         1234 c         1559 g          618 t
ORIGIN      
        1 ggaggggttt atcgcggggc tgcacaagtc cccctaccac gggttctcgg tggaattcgc
       61 cgagcaccgg cagtacatgc cgggggacaa cattcgggct atcgactgga aggtgtacgc
      121 caagagcgac cggttctata tcaagcagta cgaggaggag accaacctca aggcgtacct
      181 gctgctggac tgctcggcct cgatggccta ccattcgggg gagcggatcc gcaagctcga
      241 ctacgcgggg ttgctgtgcg gggcgctgtc gtacctgatg ctgcgccagc gggacgcggt
      301 cgggctggtg acgtacgacc ggaaaatccg gcggtacatt ccgccgcggt cgcggtcggg
      361 ccatctgcac gtgctcctga acgaaatcgc cgcgcaggcg ccctcggata ccaccgatat
      421 cagttcgacg ctgcacgaga tggcggatcg gatcaagcgg cgcgggctgg tgatcattct
      481 gtccgacctg atcgacgagg cggacaagct catctcggga ctcaagcatt ttcgctacaa
      541 caaccacgag gtgatcgtct tccacattct cgatccccgc gagcgggatt ttgcgttccc
      601 gcgcgaggcg gtgttcaagg atatggagac gggcgaggaa ctgacgacgc tgccctacca
      661 gatcaagaag gattatgcga aggcggtggc ggcgttttcg tccgaaatcg cctccgcctg
      721 ccgccaggcc gcgatcgact atcacccgat cgacacggcg atgccgttcg acaaggcgct
      781 ctatgcgttc ttgagcaagc gggaacggct gtactgagcg cacggcgagg ggagcgggtg
      841 gggaaccagg atcgggcgca aggagaccgt gatcagggtt gctgaaaatg cgcggggaag
      901 ataggagctt gatcatcctt tcaacggcgg ggacgaaaaa gtgacggctg tatgagtacg
      961 gcggcctggt cggtgctcat tctggcggcg gtgcttatcg ggcacctggt ggaggaagcg
     1021 gcaacgggat tccgccggaa atttccgctt ggtgagatgc cgatgtgggt ctttgtaatg
     1081 ggcaatgtcg ccgtgtacgg gttttgcgcc gcgaccttta ccctcgtgct gcttgcacgt
     1141 ccggcgggaa cgcttatggc atgggttctg gcggttgcga tggcactcaa cggcatcgtg
     1201 catatcggcg tgatgattta ccggaaggag tattttcccg gcgggattac ggcgggcttg
     1261 gtgctggcgg cggcggcggg attgatggcg gcgctggcgc gggggtagga ggcagggggg
     1321 atcggcgacg cagacagacc ccgccgctgc ggatgagcac tccggacacc acagacctgg
     1381 ctgagcgcac ggcgttctgg gcgatcgggg cagggcgcag caggccaatt cgggcgcgca
     1441 agcgcttcag gcgtcccgtg cggaggcgcc gggggacggc cgggcgaaga agaggcgcag
     1501 catataccac accaagccca gcgccagcac gaaacagacc aggaaaacga ggaatacatc
     1561 ccactgcggc cggatgggcc aggccgcggg gtcgaaatgc ccctcaagct ggaggaggcg
     1621 gacattgtgg cggaccacga ccatcccgag cagcgacaca aacagcatcg ccccggcgcc
     1681 gaagaatttc ctcgtgaaga agaaatggag cgacccgagc gagagaatga tcgatgcgga
     1741 gagccaccag atcgccggcg tgtgcatgaa aggcttgagg atgtccgcga gcgacaacag
     1801 gtagacgagc ccgaccgcca tcgcccccac catgccccag aggaagaagt ttcggccgag
     1861 cgcgaaagca tcctcgtgcc gccgcccgag gagaccgatg aagaatccgc cgatggtgac
     1921 ggcgccgagg agcatgtgga gccagcgcca gagccactgg ccgaggtgcg ggttgacccg
     1981 gagaccggaa gggtcgccgg cgtaaaggac ggcggcgagg ccgggacgct cggcgatcga
     2041 gaagaggttg ctgtaggcaa acgccacata cgcgagcaag aggagcgcgg cggtcagaaa
     2101 ggcgggacgc cggggggagc cgggcgcccc gaaggccgcc gcgtagagga gatagtaggc
     2161 aaccatgacg gcgccgacga tgccgaggaa gaaccaggcg gtcacgatcg cggcgctgta
     2221 cacgggcacg gggtacacca actgcacgaa cagcagggga gcgacgccga gggtgacggt
     2281 ggcggccatg gcggtgggaa acagtttgac gaactgcgcg acgacggggt ggcgccactt
     2341 gtcggcgagg cggccgaaaa cgacgatgag gagaccgccg accaggaagt tcatggcggc
     2401 gaagtggagg ctgagggtga ggaggtgcag gacagtgacc agccacagcg gggccgggag
     2461 aaaattggga tcgggcagcg ggttcatcgg gcgcctcctc gcgttgcggc cgccggcgtg
     2521 ccggcgggcg cgctcagtgt ctgcagaaac tgaatgagcg cgacgcgctc gggttcgccg
     2581 ccggtgaagg ggggcatttc ctcgccgaga tcatcggaca tgtccaggag tgtccgatac
     2641 tcctcttcgc tcagaccggc cagcgactga gtcttgtcgt tgaagccgcc gaggacgtga
     2701 caggaaccgc agcggacctc gtagacgcgg cggccgaggt cgacgccgga gagcccgcag
     2761 acctcggcga agggccggcg gtcggtgcgg gcggcgaggt gggcggccag cagccgggcc
     2821 tcctcgggcg tgcccgtcca gggtggcatg ttgccgcgca tggcgccgat cccctgcacc
     2881 atggcggtga tgaactcctc atcggcgccg tcgaaaaacg gcttcagcgc gttgtagttc
     2941 tccagcgagt ggcagctccg gcaggcgcgg cggaaaagat cggcgccttc gtcgccggtg
     3001 cgataagcca tgtgcgcgag catgccgtcg gcgcgatagt cggccgcccg ggcgccctct
     3061 accccgctcg cgtacatgta gccggtgatc acgtagggct tgcggagggc ttcgcggaac
     3121 cattcgtacg agccgaacca gaccaacccg agggccagag caaccgccgc gacttctgtg
     3181 cgccaggccc ggggagcgag gagtccgagg atggccagca gggccgcgat cgcggcggtc
     3241 agaagataga ggagatcgag ggcggcaaac gggatgttca tcgtctggcg ggcggccgcc
     3301 atgagatcgg cggggatgtc gccgacatac cagaagaggg aggggatgac gaccgcgagc
     3361 ccgacgaggc cccagaccgc attgtagcgc accaggcgca ctcgcgcgcc gggcgcggga
     3421 aggcgggcgg ccaccgcgag ggcgcagagg ccggcgagca tgatgcagat ccccgtgcgc
     3481 agcacgagcg aggaccagta ggtcgggttg aagaaaccgt cccagaatcg gccggtggcc
     3541 agccagtcgc ccggggtgag catgaaggtc acgatgccgt tgatgatgac cagggagagc
     3601 caggcggcgc cgaaatagat ccacccaatg atcaggtggc ggcgcggggt gaggcagtcc
     3661 cagccgtagt agtagagcag ggcggcgagg atctcgatga tgaagaagca ccactcggcg
     3721 gcccagcccc agacgaaatt gcggatgagg accgcggtgg cggccgggtt gagcagaccg
     3781 atgatgaacc agatgccgac cccggtgagg gcgccgaaga ccaccgtggt gaggacgaag
     3841 aaccggctca gccgccgcag gaatccgagg tggtcatcgt cgccgcggcg gcgggccgca
     3901 cgctcggcca cgaccaggta caagccgccg ccgatggcga agtgagagac gaagacgtgg
     3961 acgaccgcga tcacggccat gagcacgccg taacccaggg tcgtatccca gaagggataa
     4021 ttcatgcgtt acctccgcaa gggcaccgga caatcttgtg aaataaataa caaaacggac
     4081 cggaagaatc aactatattt cccgcaaggc ccggccaggg cagctccccg cggctaggtg
     4141 tcggccgatt tccgtttcga aaaatactcg atatcggggg cgtggtcata gccgagactg
     4201 gccacgagct ccatggaagg ttcgttccag tcttcgatca ggcaggcgat gatctcggct
     4261 ccgcgctcgt gcaggaattc ttcgca
//
LOCUS       DAOOKS010000060         4282 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_24820_length_4282_cov_3.055595, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000060 DAOOKS010000000
VERSION     DAOOKS010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24820_length_4282_cov_3.055595"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="PK112_01645"
     CDS             <1..613
                     /locus_tag="PK112_01645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HPC10783.1"
                     /translation="YATVGVHPHDATTPDADTLARLRALARHEKVVAVGEIGLDFFRD
                     RSPRETQKRAFHQQLELAIETALPVVIHTREAFRDTVAIVREYAPRLRGGVFHCFPGD
                     IAEAREAIDLGFVISVGGVITFGDTKMARVAQAVPLDRIILETDAPFLTPAPHRGKTN
                     YPAYVRYVYRRLAELKELPAAHLERTVDGTVQKLFGLVETLEG"
     gene            616..1413
                     /gene="rsmA"
                     /locus_tag="PK112_01650"
     CDS             616..1413
                     /gene="rsmA"
                     /locus_tag="PK112_01650"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HPC10784.1"
                     /translation="MPGHRPQKRLGQHFLVSAEIVQKLIDLIAASPEDVIVEIGPGRG
                     ALTVPLAASGARVVAVEFDRNLAGPLAASLRGYARTTVLNQDFLAFDPERAGLERFVL
                     VGNLPYNLTSPVVEWAVQRRAHILRAVFMVQREVAARLCAAPGGKDWSPLGIFTQLVF
                     EAQRRFDVGPEHFRPRPKVHSAVVELRPRTDAPAAPEGFEPVVRAAFARRRKQLVNNL
                     APALVPDAAAAQRAIAACGLPAQVRAEQLTTEDFFNLTRRLRSGSID"
     gene            1462..2196
                     /locus_tag="PK112_01655"
     CDS             1462..2196
                     /locus_tag="PK112_01655"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF015685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HPC10785.1"
                     /translation="MSSLTVNVNLIAMLRELRRLEEPDPAQAAVLAELNGAEGIAVQV
                     RRDRRFVRERDLYVLQAVAKTKLVVELPPADDLIGRMMEIKPHTVVFAADQPDLEAQA
                     APIDFSAPQVDFRDLTERLAAVGVQVAYLVEPEADQIKGASKARANAVLIDCGGYTRA
                     RRREEAQRELDRIDEAGRLAAKAELTVMLGRGITYRNVAPLAELGYVDDFVIGQAIAG
                     RGMLVGFGQAVREMAALVRRQEPTAN"
     gene            2209..>2556
                     /locus_tag="PK112_01660"
     CDS             2209..>2556
                     /locus_tag="PK112_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HPC10786.1"
                     /translation="MAETRELTITVPETVAAERVDRFLAARAELNLTRNRIQRLIAKG
                     LITVNGAAVAKRHEITAGDTIALVIPPPPPRAVIPEDLPLTIVYEDEFLAVVNKPAGM
                     IAHPAAGRRAGTLV"
     assembly_gap    2557..2566
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2567..3198
                     /locus_tag="PK112_01665"
     CDS             <2567..3198
                     /locus_tag="PK112_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPC10787.1"
                     /translation="ALVHRYGTLAAGSAEDRPGIVHRLDRDTTGLLVVARNDDVYLAL
                     QKKLQAREIHRHYLALVCGHVREEEGTIDAPIGRSTAGRRKMQVNGIRARQAVTHYRL
                     KDRFRSYDLLEVRIETGRTHQIRVHLAHLGHPVFGDPDYGGRESWHRGIHGPERPLAA
                     RLLAVMNRQALHAFRLAFTHPATGEALRFEAPPPEDFAELLRLLDAEGR"
     gene            3245..4078
                     /locus_tag="PK112_01670"
     CDS             3245..4078
                     /locus_tag="PK112_01670"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPC10788.1"
                     /translation="MDDTKRITEANRRAWNEATPLHQRHRTVDLRAKFAEPGYSCLDP
                     IETAKLLQIGLAGQRVAQLCCNNGREILSLVNLGAAEGVGFDISDAAIAEAEELARIA
                     GANCRFVRTDVYDIGREWDGRFDLVYITIGALSWLPDLGRFFGVAARLLAPGAALAVY
                     EQHPFLYLFPTPGDPEYDSENELTAVFSYFRTAPWEVTQGIDYIGGTTYASPTSYSHT
                     QKISDILNPILRSGLMLREFHEYPHDISLVWRHLEKYHVVPLCYLLVAHKPKDGAPAG
                     P"
     gene            4192..>4282
                     /locus_tag="PK112_01675"
     CDS             4192..>4282
                     /locus_tag="PK112_01675"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020402001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HPC10789.1"
                     /translation="MSRVKNGDTVTVHYTGRLADGTVFDSSRER"
BASE COUNT          700 a         1416 c         1503 g          653 t
ORIGIN      
        1 gtacgcgacc gtgggagtgc atccgcacga cgccacgacg ccggacgcgg acacgctggc
       61 gcgcctgcgc gcgctggccc ggcacgaaaa agtggtggcg gtcggcgaga tcgggctgga
      121 tttcttccgc gaccgctcgc cccgcgagac gcagaaacgg gccttccacc agcagcttga
      181 gctggccatc gagaccgcgc tcccggtcgt cattcacacc cgcgaggcat tccgcgatac
      241 ggttgcgatc gtgcgggagt atgctccccg gctgcgcggc ggcgtgttcc actgtttccc
      301 aggggacatc gccgaggcgc gcgaggcgat cgatctcggg ttcgtcattt ccgtcggagg
      361 ggtgatcacg ttcggcgata cgaaaatggc gcgggtggcc caggcggttc ctttggatcg
      421 gattatcctg gagaccgatg cccccttcct cacccccgcg ccgcaccgcg gcaaaaccaa
      481 ctacccggcc tatgtccggt acgtctaccg gcggctggcc gagctcaaag aactccccgc
      541 cgcgcacctc gagcggacgg tcgacgggac ggtgcagaag ctgttcgggc tggtcgagac
      601 gctggagggg tgaccatgcc ggggcatcgc ccgcagaagc ggctggggca gcacttcctg
      661 gtgtcggccg aaattgtgca gaagctgatc gatctcatcg cggcctcgcc ggaggacgtg
      721 attgtcgaga tcgggccggg acggggggcg ctgaccgtgc cgctggcggc gagcggggcg
      781 cgcgtggtgg ccgtggagtt cgaccgcaac ctggccggac cgctggccgc gtcgctccgc
      841 ggctatgcgc ggaccaccgt gctcaaccag gatttcctcg ctttcgatcc ggagcgcgcc
      901 gggttggagc gcttcgtgct cgtcgggaat ctcccctaca acctcacctc gccggtggtc
      961 gagtgggccg tgcagcgccg ggcgcacatc ctccgcgcgg tgttcatggt gcagcgggaa
     1021 gtggccgcgc ggctgtgcgc ggcgccgggg ggaaaagact ggtcgccgct gggcatattc
     1081 acgcagctgg tgttcgaggc gcagcggcgg ttcgacgtgg gaccggagca ttttcgcccg
     1141 cgaccgaaag tgcactcggc ggtcgtggag cttcgcccgc ggacggacgc gccggcggcg
     1201 ccggaggggt tcgaaccggt cgtgcgggcg gccttcgccc gacggcgcaa gcagctggtc
     1261 aacaacctcg cgcccgcgct cgtacccgac gccgccgcgg cccagcgggc gatcgccgcc
     1321 tgcggtctgc cggcacaggt gcgggccgag cagctcacca ccgaggactt tttcaatttg
     1381 acgcgccggt tgcgatccgg tagtattgac tgaccgaacg aaagctctaa cccaagcctt
     1441 gcggcgcaag cggaggtcgg cgtgtcgtca ctgacggtaa atgtaaatct catcgccatg
     1501 ctgcgcgaac tgcggcgcct ggaggagccc gatccggccc aggcggccgt gctggcggaa
     1561 ctcaacggcg cggaggggat cgcggtccag gtgcggcgcg accggcgctt cgtccgggag
     1621 cgcgacctct acgtgctcca ggcggtcgcc aaaaccaagc tggtggtcga gctgccgccg
     1681 gccgacgacc tcatcgggcg gatgatggag atcaagccgc acacggtcgt gttcgccgcg
     1741 gatcagccgg acctcgaggc gcaggccgcg ccgatcgatt tcagcgcgcc gcaggtcgat
     1801 ttccgcgatc tgaccgagcg actggccgcg gtcggtgtgc aggtcgccta cctcgtcgag
     1861 ccggaggcgg accagatcaa gggcgcctcc aaggctcggg ccaacgccgt gctgatcgac
     1921 tgcggcggct acacgcgggc ccgccggcgc gaggaggccc agcgcgaact tgaccggatc
     1981 gacgaggccg gacggctggc ggccaaagcg gagctcacgg tgatgctcgg acggggaatc
     2041 acctaccgca atgtcgcgcc cctggcggaa ctcggttatg tcgacgattt cgtgatcggg
     2101 caggcgatcg ccggccgggg gatgctggtc gggttcggac aggcggtgcg ggaaatggcg
     2161 gctctcgtgc gccgacagga accgaccgcg aactgagcgg gcggcgctat ggcggaaacc
     2221 cgcgagctca cgatcacggt tccggagacg gtggccgcgg agcgggtgga tcgtttcctg
     2281 gcggcgcggg cggagctcaa tctcacgcgc aaccggatcc agcggctgat tgccaagggg
     2341 ctgatcacgg tcaacggcgc ggccgtcgcc aagcggcacg aaatcacggc cggcgacacg
     2401 atcgcgctgg tgattccgcc gccgcctccg cgcgcggtga ttcccgagga cctccccctg
     2461 acgattgtct acgaggacga attcctcgcg gtggtgaaca agccggcggg gatgatcgcg
     2521 cacccggccg ccgggcgccg tgcggggacg ctggtcnnnn nnnnnnacgc gctcgtgcac
     2581 cgctacggga cactcgcggc gggatcggcc gaggaccgcc cggggatcgt gcaccggctc
     2641 gaccgcgaca ccaccgggct gctggtggtg gcccgcaacg acgacgtcta cctcgccctg
     2701 cagaaaaaac tccaggcgcg cgaaatccac cgccactacc tcgccctcgt gtgcgggcac
     2761 gtgcgcgagg aggaggggac gatcgacgcg ccgatcgggc gctcgacggc cggccgccgg
     2821 aagatgcagg tgaacggcat tcgggcgcgg caggcggtca cccactaccg cctgaaagac
     2881 cgcttccgct cctacgatct gctcgaggtg cgcatcgaaa cgggccggac ccaccagatc
     2941 cgcgtgcacc tggcgcacct cggccacccc gtgttcggcg acccggacta cggcggccgg
     3001 gagtcatggc atcgggggat ccacggtccg gagcggccgc tggcggcgcg cctgctggct
     3061 gtcatgaatc gtcaggcgct ccacgcgttc cggctggcgt ttacgcaccc cgccaccggc
     3121 gaggcgctcc ggttcgaggc gccgccgccg gaggatttcg ccgaactgtt gcgcctgctc
     3181 gacgccgagg ggcggtgagg gagttttcgg tggcgcccgc accgcccggg ccctatcttg
     3241 gcgcatggac gacaccaagc gcatcaccga ggccaatcgc agagcctgga acgaggcgac
     3301 gccgctccac cagcggcacc gcacggtcga tctcagagcg aaattcgccg aaccggggta
     3361 ttcctgcctc gatccgatcg aaacggcgaa actgcttcag atcggactgg cgggacagcg
     3421 cgtcgcgcaa ttgtgctgca acaacgggcg cgaaatcctc tctctggtaa acctcggggc
     3481 ggccgaggga gtcgggttcg acatctccga tgccgcgatc gccgaggccg aggaactggc
     3541 ccgcatcgcc ggcgccaact gccggttcgt gcgcaccgac gtgtacgaca tcgggcggga
     3601 gtgggacggc cggttcgacc tcgtctacat cacgattggg gcgctctcct ggctgcccga
     3661 cctcggccga tttttcggcg ttgccgcccg cctgctcgcc ccgggcgcgg ccctggcggt
     3721 ctacgaacag caccccttcc tgtatctctt cccgacgccg ggggaccccg agtacgactc
     3781 cgagaacgag ctgaccgcgg tcttctcgta ttttcggacc gcgccgtggg aggtgacgca
     3841 ggggatcgac tacatcggcg gcaccaccta tgcgagtccg accagctaca gccacacgca
     3901 gaagatttcg gatattctca atccgatcct cagaagcggg ctgatgctga gggagtttca
     3961 cgagtacccg cacgacatat cgctcgtgtg gcgccatttg gagaaatacc acgttgtccc
     4021 gctgtgctac ctgctggtgg cgcacaagcc gaaggacggt gctccggcgg ggccatgaca
     4081 atcgcgcatc gcagtctggg ctgctgaggg tttcggcgcc gtcatgcagt gacgggatct
     4141 cagaagtctg ggccggataa tgatcagagc ggaaccagac aggaggacga catgagccgg
     4201 gtgaaaaacg gagataccgt caccgtccac tacacggggc ggctggccga tggaacagta
     4261 ttcgacagct cgcgcgaacg cg
//
LOCUS       DAOOKS010000061         4239 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_25158_length_4239_cov_4.182361, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000061 DAOOKS010000000
VERSION     DAOOKS010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4239
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25158_length_4239_cov_4.182361"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1072)
                     /locus_tag="PK112_01680"
     CDS             complement(<1..1072)
                     /locus_tag="PK112_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HPC10790.1"
                     /translation="MRPLTAHRTTASLVTLLAPLILASCSEAGPAARATAPDGDLGKI
                     SLPPGFSISYYADRMPGARSMRLGDNGTLFVGTRGDKVYALRDVDGDYRADSLYVIAT
                     GLDMPNGVAFRDGSLYVAEVSRVIRLDGIESRLSDPPTPAVVTDTFPSDRAHGWKFIG
                     FGPDGRLYVPVGFPCNVCEPDEDICGTIVRMDPDGGRAETFARGVRNTVGFDWEPTSG
                     VLWFTDNGRDRMGDDIPPDELNRAPSAGMHFGFPYCHAGDIPDPQFGQGADCDTFAAP
                     VRKLGPHAAALGMRFYTGAMFPEKYRGQIFIAEHGSWNRSTPLGYRVMLVTHDGEGHA
                     TSYEVFAEGWLEDNGARWGRPVD"
     gene            1244..2059
                     /locus_tag="PK112_01685"
     CDS             1244..2059
                     /locus_tag="PK112_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015904149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HPC10791.1"
                     /translation="MDRVNDRLLEWGAIYGTKIIGALVILIVGYLLTKFLTAAVRRIT
                     TRAQVDAALVKFLATVTKVMLLVFVILAAMNSLGVQTTSFIALIGAAGLAVGLALQGS
                     LANFAAGVMLILFRPFKGGDYVETAGVAGTVEEIHIFHTVLRTPDNKKVTVPNSAVTG
                     GNIINYSAHDTRRIDMVFTVGYRDDLRLAKRTLEEILAADPRVLKDPPPTVAVGQLAE
                     SAVNIVVRPWVRTEHYWPVLFDVHERVKERFDEQGVTIPYPQRDIHVHQTRVS"
     gene            2074..2976
                     /locus_tag="PK112_01690"
     CDS             2074..2976
                     /locus_tag="PK112_01690"
                     /inference="COORDINATES: protein motif:HMM:NF022720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HPC10792.1"
                     /translation="MSRTARITIAALAMVLGAAGAVAAQEATVDSTFIWKRALAVDLT
                     ATQTAYSDSWTGGEAGAFSWVGNAAGFAERNLNPWFNLRSQVKLSFGQTTTQDEETKD
                     WSKPKKSTDLIDWETVGRFLLNQYVDPYAAFRLESQFYDGSNAKKKLYLSPLKLTESA
                     GLAHRFYEKSNDHITTRLGGAYRQIITRFIADTVTLATDSRTVSDAGLESVTDAVLTL
                     SEKMQYTGKLTLFKALYNSEDDALGADDDWKAVDVRWENAVTAQVSKIIAVSFLFDLR
                     YDKEIVDKAQIKETLGLGFLFKLI"
     gene            3113..>4239
                     /locus_tag="PK112_01695"
     CDS             3113..>4239
                     /locus_tag="PK112_01695"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HPC10793.1"
                     /translation="MRIVAAVAALTAVVAAGAAQAGLTDPYAILERSVAASGGLDNIR
                     SHETSYAEGTIEIVGSGLAGPFRQWQRRPELQRLEVELGPLAITQGNNGEVAWTVDQN
                     GKVQIHRDELTVGQEKADSLMAEFAHLDRGSAVFAVTYDGLDTAAGAACYVVTITNTL
                     SLDTTRLYIDTASFRQRKSAALTASGQTITVYDDFRTVAGVEMPFSQRQVTLPVNMVQ
                     NIVMTKYEANVPIEPAMFNPPSEDVSDVRFADGDRAEDIPFRFIENHIYVPVTVKGKE
                     RLWILDSGAEMTVVEKRFAEEIGLETQGNITGKGAGNLVDVTFTTVPMLSIDDRVLVD
                     EQKVAVIDLYWFFQEALGLEIAGILGYDFLSRVVTSVDYAG"
BASE COUNT          767 a         1478 c         1292 g          702 t
ORIGIN      
        1 tatccactgg ccgcccccac cgcgccccgt tgtcctccag ccacccttcc gcgaacactt
       61 cgtacgaggt cgcgtgcccc tcgccgtcgt gcgtcaccag catcacccgg tacccgagcg
      121 gtgtgctccg gttccaggag ccgtgctcgg cgatgaatat ctggccgcga tacttctccg
      181 gaaacatcgc cccggtgtag aaccgcatgc caagggctgc cgcgtggggt ccgagtttcc
      241 gcaccggcgc ggcgaaggtg tcgcagtccg ccccctggcc gaactgcggg tccgggatgt
      301 ccccggcatg gcagtagggg aacccgaaat gcatcccggc cgagggcgcc cggttcaatt
      361 cgtccggcgg gatgtcgtcg cccatccggt cccgcccatt gtcggtaaac cagagcacgc
      421 cgctggtcgg ctcccagtcg aacccgaccg tgttgcgcac gccgcgcgcg aaggtttcag
      481 cccgcccccc atcgggatcc atccgtacaa tcgtcccgca gatgtcctca tcgggctcgc
      541 acacgttgca cggaaacccg accggcacgt acaaccggcc atcggggccg aaaccgataa
      601 acttccaccc gtgcgcccgg tcgctcggaa atgtatcggt gaccactgcc ggcgtcggcg
      661 gatcgctcag gcgcgattcg atcccatcca ggcgaatgac ccggctcacc tcggcgacat
      721 acagactgcc gtcccggaac gccacgccgt tgggcatgtc gagcccggtc gcgatcacat
      781 acagcgagtc cgcccggtag tcgccgtcga cgtcgcgcag cgcgtacacc ttgtccccgc
      841 gcgtcccgac aaagagcgtg ccattgtccc cgagccgcat cgaccgcgcc cccggcatcc
      901 ggtcggcgta gtagctgatc gaaaatccgg ggggcagact gattttcccc aggtcgccgt
      961 ccggcgcagt agcgcgggcg gccgggccgg cctccgagca ggaggcgaga ataagggggg
     1021 cgagcagagt gacgagcgag gcggtcgtgc ggtgcgcggt gagcggtctc atgcggtatc
     1081 ctccgggcgg ttgatcgtct ccgtctgtac aaccgcgccc gcgggcgacg gttcctgtca
     1141 ttttcccgcc ggaccggcgg gccgccggaa acgcttgacg aggccgtacg gcgcatggta
     1201 gtttcccggc agcctcggaa tgcgaaacgg aaaggtgcgg gttatggata gagtgaatga
     1261 ccggttgctt gagtggggcg cgatttacgg cacgaaaatc atcggcgccc tcgtcatcct
     1321 gatcgtcggc tacctgctca cgaaattcct caccgcggcg gtccgccgca tcacgacccg
     1381 cgcccaggtg gatgccgcgc tggtcaagtt cctcgcgacc gtcaccaagg tgatgctcct
     1441 cgtcttcgtc atcctcgcgg cgatgaacag cctcggggtg cagacgacct cgttcatcgc
     1501 cctgatcggc gcggccggtc tggccgtcgg cctcgcgctg cagggctcgc tggcgaactt
     1561 cgccgccggc gtcatgctca tcctcttccg ccccttcaag ggcggcgact acgtcgagac
     1621 ggccggcgtc gccggtacgg tcgaggagat ccacatcttc cacaccgttc tccggacgcc
     1681 cgacaacaag aaagtgacgg tgcccaacag cgccgtcacc ggcggcaaca tcatcaacta
     1741 ctccgcccac gacacgcggc ggatcgacat ggtgttcaca gtcggctacc gcgacgacct
     1801 ccggcttgcc aagcggacac tcgaggagat cctcgcggcc gacccgcgcg tcctgaaaga
     1861 cccgcccccg accgtggccg tcggccagct ggccgagagc gcggtgaata ttgtcgtccg
     1921 cccctgggtg cgcaccgaac actactggcc ggtgctgttc gatgtccacg agcgggtcaa
     1981 ggagcggttc gacgagcagg gggtcacgat cccgtacccg cagcgcgata ttcacgtcca
     2041 tcaaacccgt gttagctgac ggaggttatc cgtatgtccc gtaccgcccg gatcacgatt
     2101 gccgcgctcg ccatggttct cggcgcagcc ggcgcagtcg cggcgcagga ggcgaccgtc
     2161 gactcgacct tcatctggaa acgcgcgctc gccgtcgacc tgaccgccac gcagaccgcc
     2221 tattccgact cctggaccgg gggagaggcc ggcgcgttca gctgggtcgg caacgccgcc
     2281 ggtttcgccg agcgcaacct caacccgtgg ttcaacctgc gctcgcaggt caagctctcg
     2341 ttcgggcaga cgaccactca ggacgaggag accaaggatt ggtccaagcc gaagaaatcc
     2401 accgacctga tcgactggga aacggtcggc cgcttcctcc tcaaccagta cgtcgatccc
     2461 tacgccgctt tccgcctcga gtcgcagttc tacgacggct ccaatgcgaa gaagaagctc
     2521 tacctgtcgc cgctcaagct gaccgagtcg gccggactgg cccaccggtt ctatgagaag
     2581 agcaacgacc acatcaccac gcgcctgggc ggcgcctacc gtcagatcat cacccgcttc
     2641 attgccgaca ccgtcaccct cgccaccgac tcccgcaccg tctccgacgc cggcctggaa
     2701 tcggtgaccg atgccgtgct gacgctcagc gagaagatgc agtacacggg caagctcacg
     2761 ctgttcaagg cgctgtataa ttcggaggac gacgcgctcg gcgccgacga cgactggaaa
     2821 gcggtcgatg tccgctggga aaacgccgtc accgcccagg tcagcaagat tatcgccgtg
     2881 agcttcctct tcgatctgcg ctacgacaag gagatcgtcg acaaggccca gatcaaggag
     2941 accctgggac tcgggttcct gttcaagctg atctagttcg cgaacccgat cccttcacat
     3001 ccccgggccc cggcccgggg atttgtgggt gcgaggccga acaaagcgtc ggctctcccg
     3061 tactgacagg tcaagacgca caaaccgacc gactcatcat ggaggtgcct tcatgcgaat
     3121 tgttgctgcc gtcgccgccc tgacggctgt cgtcgcggcc ggcgccgcgc aggccggcct
     3181 cactgatccc tacgccatcc tggagcgcag cgtcgctgcc tccggcggcc tcgacaacat
     3241 ccgcagccat gagaccagct acgccgaggg gacgatcgag atcgtcggct ccggcctcgc
     3301 cggaccgttt cgccagtggc agcgtcgccc cgaactgcag cgcctcgagg tcgaactggg
     3361 accacttgcc atcacccagg ggaacaacgg cgaggtcgcc tggaccgtcg accagaacgg
     3421 caaagtgcag attcaccggg acgagctgac ggtcggccag gagaaggccg acagtctcat
     3481 ggcggagttc gcccacctcg accgcggcag cgcggtgttc gccgtgacct atgacggcct
     3541 cgacactgcc gccggcgccg cctgctacgt cgtcaccatc accaacactc tctcgctcga
     3601 caccacgagg ctctatatcg acaccgcctc cttccgccag cggaaatcgg cggctctcac
     3661 cgcaagcggg cagacgatca ccgtctacga cgatttccgc acagtcgcgg gcgtcgagat
     3721 gccgttctcc cagcgccagg tcacgctccc ggtgaacatg gtccagaaca tcgtgatgac
     3781 caagtacgag gcgaatgtgc cgattgaacc ggcaatgttc aacccgccga gcgaggacgt
     3841 ctccgacgtg cgcttcgccg acggcgaccg ggccgaggat atcccgttcc ggttcatcga
     3901 aaatcacatc tacgtgccgg ttacggtcaa gggcaaagag cgtctctgga tcctcgactc
     3961 gggcgcggag atgacagtgg tcgagaagcg cttcgccgag gaaatcgggc tcgagacgca
     4021 ggggaacatc accggcaaag gggcgggcaa tctcgtcgac gtcaccttca cgaccgtccc
     4081 gatgctcagt atcgacgacc gcgttttggt cgacgaacag aaagtcgccg tcatcgacct
     4141 ctactggttc ttccaggagg cgctcggact ggagatcgcc ggcattctcg gctacgattt
     4201 cctctcgcgc gtggtcacga gtgtcgacta cgcgggcga
//
LOCUS       DAOOKS010000062         4238 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_25171_length_4238_cov_3.720296, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000062 DAOOKS010000000
VERSION     DAOOKS010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4238
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25171_length_4238_cov_3.720296"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="PK112_01700"
     CDS             <1..366
                     /locus_tag="PK112_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPC10794.1"
                     /translation="GVSILDAMDITARTAGNRVIANAINKSVMAIAEGDTITGPLKES
                     GVFPPMVTQMISVGEKTGGLDEMLNKIAEFYDEEVDDAVSALTSIIEPVIIVFMGIVI
                     GGILIAMYLPMFDIIGKIG"
     gene            463..2169
                     /locus_tag="PK112_01705"
     CDS             463..2169
                     /locus_tag="PK112_01705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPC10795.1"
                     /translation="MVSAEHPRRLAWFIPLRLATFLVVFFVVVFWMKFPGFLALPFFL
                     YAGVTLGFTLLLALDKQQRLGAVTTAMVALHFLLEIGVESGIIYATGNVNSPFSALFV
                     LTIVSAALVYRLVGTLVIASTVALAYAFIVWLGLTNSDPEWSLRALRTVFDTQETAFY
                     SIFLHLLIFYLVAFIAGYLGQRLRDQDRRLADASRALRRARLETDDILRHLNSGLITI
                     DAEGCIVYFNRTAERILGYCEEDIKGLSCDQAFADRMPALARSLMDGVRRRVAHLRQE
                     LEIINPLGRRVPLGLSISILTEEDKATPRGVIAIFSDLTEAKDLEAKVRAADRLAAVG
                     ELSASIAHEIRNPLAAISGSVEVLKSALPVAGENARLMDLIVKESDRLTKILSEFLLY
                     ARIDRPSYTKVDLCRLTSDVLHILSHHESRHPGIRLAFDADQSVVYVAGDEDLLTQVL
                     LNLGVNACEAVGAAPGDVHFRVFSRDPERVVLQVADTGCGMAPEVMRRMFQPFFSTKR
                     QGTGLGLAIVHRVCTALKIGLAVDSAPGRGATVTLEFPVCMPRPQLQGAATASAAPAA
                     VR"
     gene            2257..4026
                     /locus_tag="PK112_01710"
     CDS             2257..4026
                     /locus_tag="PK112_01710"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HPC10796.1"
                     /translation="MMALRTYARLTGVLALVIAACGEPPTLTHESVDRFDRVVARVDT
                     VYTLTFPEFYRKLEESILLPDGGRLSQREVADELDRVVMDTLAGLVADTVNLGANYEL
                     NRIYRQRYYEYLLRRFGQVAFFEKTSVDSAAVRQYYEQNLAQFTTPEEVDVSHILIAA
                     GNLPHDRDSLKYRGLTQPELAAAAEKHAWYVKSLLDQGHPFDSIAREYSHDNLIASYG
                     PRIGWVRRGVYEHPFDSVAFGLAPGRYSDPYFASNSWHLIYVAAYHSDTTAPLEGTIA
                     DQVTVHLRTAAADSLARPFLDSLDAAMQLEINEAILDSNVFLLPKPLWAAVVNGRDTV
                     DMADLQSLEEGFRKRHRVDNTTPDMKREMIQIAARRYALVQAARAAGIEELPDVREQE
                     FKLRRQYQKTIVLRQGLDDGWTPSDAEIEAYYRRNYRETPIDKPLVVQHIIAPDSASA
                     AFLRDQALAGHDFLALAEQYYPGDSSLRRDLADLGPIGPQDVSPEFYEAARVTPDGNV
                     SFPVQTQYGWHVIKVLEHRTPITLDKVRTQIRQTLRDQHLREITTTFRDRLYRRFAVS
                     FPNRLPAVDLRPIRDRQEGQDGQ"
     gene            4016..>4238
                     /locus_tag="PK112_01715"
     CDS             4016..>4238
                     /locus_tag="PK112_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10797.1"
                     /translation="MDSDVTVSAPPSAPPPISFRSELARKATHMFALVIPAGYYVLGL
                     GRGTMLAVMIPVALCTFFVDLARLQGRPLW"
BASE COUNT          670 a         1570 c         1231 g          767 t
ORIGIN      
        1 ggcgtctcca tccttgacgc catggacatc accgcccgga ccgccggcaa ccgcgtcatc
       61 gccaacgcca tcaacaagtc ggtcatggcc atcgctgagg gcgacacgat caccgggccg
      121 ctcaaggaat cgggcgtgtt tccgccgatg gtgacccaga tgatctctgt cggcgagaag
      181 accggcggcc tcgacgaaat gctcaacaag atcgccgagt tctacgatga ggaagtcgac
      241 gacgcggtct cggctctgac ctcgatcatc gagcccgtca tcatcgtctt catgggtatc
      301 gtcatcggcg gcatcctcat cgccatgtac ctgccgatgt tcgacatcat cgggaagatc
      361 ggctgatccc gagatcccgc cgccggcgct gggccggccg cggacaccga atgaccctgc
      421 tggacggcgc cctccgcggc gccgtccggc gtgcgggaaa ccatggttag cgccgaacac
      481 ccccgccgtc tcgcgtggtt catccccctc cggttggcca ctttcctcgt ggtcttcttc
      541 gtcgtcgtct tctggatgaa attccccggt ttcctcgccc tgccgttctt cctctacgcc
      601 ggcgtcaccc tcggcttcac gctcctgctg gccctggaca agcagcagcg gctcggcgcc
      661 gtgaccaccg ccatggtggc gctgcacttc ctgttggaga tcggcgtcga atccggcatc
      721 atctacgcca cgggcaacgt gaactccccg ttctcggctc tctttgtcct gacgatcgtc
      781 tcggccgcgc ttgtctaccg tctggtcggt accctcgtca tcgcctccac cgtcgccctg
      841 gcctacgcct tcatcgtctg gctcggtctg accaacagcg atcccgagtg gtcgctccgg
      901 gcgctgcgca cggtcttcga cacccaggaa accgccttct actcgatctt tctgcacttg
      961 ctgattttct atctcgtcgc cttcatcgcc ggctacctcg gccagcgcct gcgcgaccag
     1021 gatcgccgcc tcgccgacgc ctcccgcgcc cttcgccgcg cccgcctgga gaccgacgat
     1081 atcctgcgcc acctcaattc cgggctcatc acgattgacg ccgagggctg cattgtctat
     1141 ttcaaccgca ccgccgagcg catcctcggg tattgcgagg aggacatcaa gggtctcagc
     1201 tgcgaccagg ccttcgccga ccgcatgccg gccctggccc ggagcctgat ggacggcgtc
     1261 cggcggcggg tcgcccacct gcgccaggag ctcgagatca tcaatccgct gggccgccgc
     1321 gttcccctcg ggctgtcgat ctccattctg accgaggagg acaaggccac gccgcgcggc
     1381 gtcatcgcca tcttctccga tctcaccgag gccaaggacc tggaggccaa ggtccgcgcg
     1441 gccgaccgcc tcgccgccgt gggcgagctc tccgcctcca tcgcgcacga aatccgcaat
     1501 ccgctcgccg ccatctccgg ctccgtcgag gtgctcaaga gcgccctccc ggtcgcgggg
     1561 gagaacgccc gcctcatgga cctgatcgtc aaggagtccg accggctgac caagatcctc
     1621 agcgaattcc tcctctacgc ccgcatcgac cggcccagct acaccaaggt ggacctgtgc
     1681 cgcctgacca gcgacgtcct ccatatcctc tcccaccacg agtcccgcca ccccggcatc
     1741 cgcctggctt tcgatgccga tcaatcggtg gtgtacgtgg cgggagatga agacctgctc
     1801 acccaggtgc tgctgaacct cggggtcaac gcctgcgagg cggtcggggc cgctcccggc
     1861 gacgtacact tccgcgtctt ctcccgcgat ccggagagag tcgtgctgca ggtcgccgac
     1921 accgggtgcg gcatggcacc ggaagtcatg cggcgcatgt tccagccgtt cttttcgacc
     1981 aagcgccagg ggaccggcct gggcctggcg attgtccacc gcgtctgcac tgccctcaag
     2041 atcggcctcg ccgtcgactc cgctcccggc cggggggcca cggtcacgct cgaattcccg
     2101 gtgtgcatgc cgcggcccca gctccagggc gccgcgaccg cctcggcggc ccccgccgcc
     2161 gtccgctgac cgcccgattt tggttggctt ttctccgccc gttgtataac ttaatcagcc
     2221 aaccggatcc cgtgtgatcg caaccgtgac ggagagatga tggccttacg aacctatgcc
     2281 cggctgacgg gcgtcctcgc actcgtgatc gccgcctgcg gcgagccgcc gacgctcacc
     2341 cacgaatccg tcgaccgctt tgaccgggtc gtcgcccgcg tcgataccgt ctacaccctc
     2401 accttccccg agttctaccg gaaactggaa gagagcattc tgctgcccga cggcggccgg
     2461 ctgtcgcagc gggaggtggc cgacgaactg gatcgcgtcg tcatggacac gctcgccggt
     2521 ctggtcgccg acaccgtcaa cctcggcgcc aactacgagc tcaaccgcat ctaccgccag
     2581 cggtactacg agtaccttct ccgccgtttt ggccaggtgg ctttctttga aaagacctcg
     2641 gtcgattccg cggccgtgcg gcagtattac gaacagaacc tcgcgcagtt cacgaccccg
     2701 gaggaggtcg acgtttcgca cattctgatc gccgccggga acctgcccca cgaccgcgat
     2761 tcgctgaagt atcgcggcct cacccagccg gaactggcgg cggcggccga gaagcatgcc
     2821 tggtacgtga aatccctgct cgaccagggt catcccttcg attccatcgc ccgcgagtat
     2881 tcccacgaca acctgatcgc ttcctacggc ccgcggatcg gctgggtccg gcggggcgtc
     2941 tatgaacacc ccttcgattc ggtcgctttc ggtctcgccc cgggccggta ctccgatccg
     3001 tatttcgcct cgaacagctg gcatcttatt tacgtcgccg cttatcactc cgacaccacc
     3061 gccccgctgg aggggacgat cgccgaccag gtcaccgtcc atctccgcac ggcggccgcc
     3121 gactccctcg cccgtccgtt cctggactcg ctcgatgcgg ccatgcagct tgaaatcaac
     3181 gaggccatcc ttgacagcaa tgtcttcctg ttgcccaagc cgctgtgggc ggcggtggtc
     3241 aacggccgcg atacggtcga catggccgac ctccagagcc tcgaggaggg attccggaag
     3301 cgccaccggg tggacaacac cacccccgac atgaagcggg agatgatcca gattgccgcc
     3361 cggcggtatg cgctggtgca ggcggcccgc gccgccggga tcgaagaact cccggacgtg
     3421 agggagcagg agttcaagct ccgccgccag taccagaaga cgatcgtcct gcggcagggg
     3481 ctggatgacg gctggacgcc gtccgacgcc gagatcgagg cctactatcg ccggaactac
     3541 cgcgagacgc cgatcgacaa acccctcgtc gtccagcaca tcatcgcgcc cgactccgcg
     3601 tcggccgctt tcctccgcga ccaggcgctc gccggccacg atttcctcgc tctcgccgag
     3661 cagtactacc ccggcgattc gagtctccgc cgcgacctcg ccgacctcgg gccgatcggg
     3721 ccgcaggacg tgtccccgga attctatgag gcggcccggg tgacgcccga tggcaacgtc
     3781 agctttcccg tccaaaccca gtacgggtgg cacgtcatca aagtgctgga gcatcgcacg
     3841 ccgataactc tggacaaagt gcggacgcag atccggcaga ccctgcgcga tcagcacctc
     3901 cgcgagatca ccacgacttt ccgcgaccgg ctctaccgcc gcttcgccgt ctccttcccg
     3961 aatcgcctgc cggcggttga cctccgcccc atacgcgacc ggcaggaggg gcaggatgga
     4021 cagtgatgtg acagtctcgg cgccgccgtc cgctccgccg ccgatcagct ttcgcagcga
     4081 gctggcgcgc aaagccacgc acatgttcgc actcgtcatc ccggccggct actacgttct
     4141 cggtctgggg cgcgggacca tgctcgcggt catgatcccg gtggccctgt gcaccttctt
     4201 tgtcgatctc gcgcgcctgc aggggcgccc gctctggc
//
LOCUS       DAOOKS010000063         4232 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_25215_length_4232_cov_2.907350, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000063 DAOOKS010000000
VERSION     DAOOKS010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4232
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25215_length_4232_cov_2.907350"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="PK112_01720"
     CDS             <1..671
                     /locus_tag="PK112_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10798.1"
                     /translation="DGATMVTNPPLPGIIPGTISHLFVSGAVVRPISDFEVGLDLPPS
                     LGAGVSYQATDRLVLALDGEYTFWSRFDGLDFAFSNAVGLAGPADTVPLAREFFTADL
                     SAPVDWDNSVKLMLGASYDFPEFLAPEVGLTLVGGLSWDQSPARNEWRLTPQLLDTGN
                     KFGFSGGGIVHLDRWDLGLVTSYTRLQHQRAWDLDMDEAFTSFPGYYQPQTFETILSF
                     NYRF"
     gene            692..2017
                     /locus_tag="PK112_01725"
     CDS             692..2017
                     /locus_tag="PK112_01725"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HPC10799.1"
                     /translation="MTRYLLAALILVLTLATGCSDRGDNTGPVYDGESGVLTADHMFF
                     QELSTQIRNQGRWMLYLKAWVPKYNEWGPPPNYHPDHPLPLLVLLPPEDADEWYYFHH
                     GLSQVANELMAEGLLEPMAIFVPQNDPVLGGYWWAGSGGGCGNYDTLVGGTLIDHIRE
                     NALGPGAFDTAQCMTAIGGIGTGAYGAVRAALKHPGRFGSISVINGPMDFDGLDGQGG
                     LIPLFAKAIAEQSGVPFATKWDTSSYNEISRLLTGGAMAFSPHDTLVYPVITYTQTGP
                     RVACPDSAKITGTDTWVRTRYKIAGTETLLPDLISAADFSFQVHLPFDRFGRPYSAPN
                     EPIWDLWLRNSPDSMIYNAESQGRAFPNTHFWVAYSREDDPLGYNVMTRDFAKLLQKY
                     RYDPEIYYFGGYEGNPGWGDEYLTHLLREVLIFHSNCFRQARQNQQGGN"
     gene            2091..2813
                     /locus_tag="PK112_01730"
     CDS             2091..2813
                     /locus_tag="PK112_01730"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HPC10800.1"
                     /translation="MAETTPYDLLSVGAHPDDVEVGTGGVLIDAARRGYKVAVAILTE
                     GELGTGGTAEIRRREVEEAAGILGAEVVARFDWGDTRLEDTYEHRRALAEVLRRTRPR
                     IVLAPYPHVGHGRRQSHPDHVAAGIIAINAANLAALKKADVPGEPHLVTRIFHYFLPP
                     GVSPNFVVDITPHFDRWIQALSAHRSQFLNPEKSRDYLEGIAATSRSFGLLTRVKYGQ
                     GFHAVEPILVKDIMTLAESWEG"
     gene            2949..>4232
                     /locus_tag="PK112_01735"
     CDS             2949..>4232
                     /locus_tag="PK112_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10801.1"
                     /translation="MTCRASASTVLVAVVLLAISVVARSPLPPVESDPGRPALPPLAL
                     SPDSVCDPGVVWPTAYGVQQTGEINAAFTAAGKFGLRFFYGPWVDSWPSQGFETPAYS
                     GVDYLFTGGLWVGGVVGNDTLVSVTYDGWVNVEEFFPPRLVPEVDRFPAVSDFGLRTS
                     FTDTCASCPSEDPIDSTHRPLDVKVALRSHAWRTPPLDKHIFYDAVITNIGSELIEAG
                     YVGLYVDGDVVWVGSTGEGWTDDLAGAVREVGTAYIIDNNGDFGSGEPEKLRALAARF
                     LNASQSPSDTNCNWWISNGYAELDFGPRLRGAPDDPFRDFGTGGLGTPTGDANKYYVL
                     RHREWDYDQFATAAIDSADPLWLTPPAALAVDLCNGYDARFLLSLGPFTLAPDSSFRV
                     IFSMFTADSIHTVPGNLAHLPADPAAYRDNLGLERL"
BASE COUNT          689 a         1457 c         1322 g          764 t
ORIGIN      
        1 cggacggcgc gaccatggtg accaacccgc cgctgcccgg gatcatcccc ggcaccatca
       61 gccacctgtt cgtctcgggg gccgtggtgc ggccgatctc cgatttcgag gtgggtctcg
      121 atctgccgcc gagcctgggc gccggcgtgt cctaccaggc gaccgaccgg ctggtgctgg
      181 cgctcgacgg cgagtacacg ttctggtcgc gctttgacgg actcgacttc gcgttctcga
      241 acgccgtggg cctggccggt ccggccgaca cggtgccgct ggcccgcgag ttcttcaccg
      301 ccgacctcag cgcaccggtc gactgggaca attcggtgaa gctgatgctc ggagcctcat
      361 atgactttcc ggaattcctg gcgcccgagg tggggttgac gctggtgggc gggctgtcgt
      421 gggatcagtc gcccgcgcgc aacgagtggc gcctgacccc gcaactgctc gacaccggca
      481 ataagttcgg cttctccggc ggcggcatcg tgcacctcga ccgctgggat ctcggcctcg
      541 tcacctcgta cacacgtctg cagcaccagc gggcctggga ccttgacatg gacgaagctt
      601 tcaccagctt ccccggctac taccagccgc agacttttga aaccatcctc tcgttcaact
      661 accggttcta gggaggtcgg agatgcaagc aatgacgaga tacctactgg ctgctttgat
      721 tctggtgctc acgctggcga cgggctgctc cgaccggggt gacaacaccg gacctgtata
      781 tgacggcgag agcggcgtgc tgaccgccga tcacatgttc ttccaggaac tgtccaccca
      841 gatccgcaac cagggtcgtt ggatgctcta cctgaaagcc tgggtgccga agtacaatga
      901 gtggggcccg ccgccgaact accacccgga ccacccgctt cccctcctgg tgctcctgcc
      961 gccggaagac gccgacgagt ggtactattt tcaccacggc ctctcgcagg tggccaatga
     1021 gttgatggcg gaagggctcc ttgagccgat ggccatcttc gtcccccaga acgacccggt
     1081 tctgggcggc tactggtggg cgggatcggg cggcggctgc ggcaattatg acactctggt
     1141 cggcggcacg ctgatcgacc acatccggga gaatgcgctc ggccccggcg ctttcgacac
     1201 ggcgcagtgc atgaccgcca tcggcgggat cggcaccggc gcctatggcg ccgtccgcgc
     1261 cgccctcaaa caccccggac gcttcggatc catcagcgtc atcaacggcc cgatggattt
     1321 cgacggcctc gacggccagg gcggcctgat ccccctgttc gcaaaggcca tcgccgaaca
     1381 gtccggcgta cccttcgcca ctaagtggga cacctccagc tacaacgaga tcagccgtct
     1441 cctcaccggc ggcgctatgg ctttttcccc gcacgacacc ctcgtgtacc cggtgatcac
     1501 gtacacccag actgggccca gagtcgcctg tcccgactcc gcgaagatca cggggaccga
     1561 tacgtgggtg cgcacccgct acaaaattgc aggtacggag acgctcctgc ccgatctcat
     1621 cagcgccgcc gatttctcgt ttcaggtcca cctcccgttt gatcggttcg gccggcccta
     1681 cagcgcgccc aacgaaccga tctgggacct gtggctgcgc aacagcccgg actcgatgat
     1741 ctacaacgcc gaaagccagg ggcgggcctt tcccaacacc cacttctggg tggcctactc
     1801 ccgggaggac gaccccctcg ggtacaatgt catgacccgg gacttcgcca agctgctgca
     1861 gaagtaccgg tatgatcccg agatttacta cttcggaggc tacgagggca acccggggtg
     1921 gggcgacgag tacctcaccc acctgctgcg cgaagtgctc atcttccact cgaactgctt
     1981 ccgtcaggcc cgccagaacc agcagggcgg gaactgaccc gatttgaccg gcacatgcgc
     2041 ccggagaact cgtttctccg ggcttttttg tagaaggaaa ggacaggcgg atggctgaga
     2101 caacccccta cgatctgctt tccgtgggag cacaccccga cgacgtcgag gtggggaccg
     2161 gcggcgtgct gatcgacgcc gcccggcggg gctacaaagt cgccgtcgcc attctcaccg
     2221 agggggaact gggcaccggc ggcaccgccg agatccggcg gcgggaggtg gaggaggcgg
     2281 ccgggatcct gggcgccgag gtcgtcgccc gctttgactg gggggacacc cggctggagg
     2341 acacctatga acaccggcgg gccctggcgg aggttctgcg gcgcacccgt ccccgcatcg
     2401 tcctggcccc gtacccgcac gtcggccacg gccggcgcca gtcgcacccg gatcatgtcg
     2461 ccgccggcat catcgccatc aacgccgcca acctcgcggc gctgaagaaa gccgacgtcc
     2521 ccggcgaacc ccacctggtc acgcgcatct tccactactt cctgcccccc ggcgtttccc
     2581 ccaacttcgt cgtcgacatc acgccgcact tcgaccgctg gatccaggcc ctctcggccc
     2641 accgctcgca gtttttgaac cccgagaagt cgcgcgacta cctcgagggg attgccgcca
     2701 ccagccgctc gttcgggctc ctgacgcgtg tgaagtacgg ccaggggttc catgccgtcg
     2761 agccaatcct ggtcaaggac atcatgacgc tcgcggagag ctgggagggc taggcggcga
     2821 ggggcgcggc ggccgcggag gggaacatgg agagattggc cttgctttcg gtgcgccggt
     2881 cgtgtttctt ggcgcaaaac cggtccaacc gctgaatcgg ccgcagggcc cccaaggagg
     2941 tctctcccat gacatgccgc gcatccgcgt cgacggtgct ggttgccgtc gtgctcctgg
     3001 cgatatcggt cgtcgcccgc agtccgctgc cgcccgtcga gtccgacccg gggcggcccg
     3061 cactcccgcc cctggcgctt tctcccgatt ccgtctgcga tcccggtgtg gtctggccga
     3121 cggcctacgg cgtccagcag acaggggaga tcaatgccgc gttcacggcc gccggtaagt
     3181 tcggccttag gttcttctat ggtccgtggg tggattcatg gccgtcccag ggattcgaaa
     3241 cgcccgccta cagcggagtc gattacctgt tcaccggggg cctctgggtt ggcggtgtcg
     3301 tcggcaacga caccctcgtg tcggttacat acgacggttg ggtaaacgtg gaggagtttt
     3361 tcccccctcg gctcgtgccg gaggttgatc gttttcccgc cgtgtcggat ttcggcctgc
     3421 ggacgtcgtt caccgatacg tgcgcatcgt gcccgagcga ggatccgatc gacagcactc
     3481 accggccgct ggacgtgaaa gtcgcgttgc gcagccacgc ctggcgcacc ccgcccctgg
     3541 acaaacacat cttctatgac gcagtcatca ccaacatcgg cagcgaactg atcgaggcgg
     3601 gttatgtcgg gctgtacgtg gacggcgacg tcgtttgggt cggaagcacg ggtgaggggt
     3661 ggactgacga tctcgccgga gcggtgcggg aggtcggcac ggcgtacatc atcgataaca
     3721 acggggattt cgggtcgggt gagccggaga agttgcgggc gcttgcggcc cgcttcctga
     3781 acgcctccca gtccccgtcc gacacaaact gcaactggtg gatctccaac gggtatgccg
     3841 aactcgattt cgggccccgg ctgcgcggcg ccccggatga ccctttccgt gatttcggca
     3901 ccggcggtct cggcacgccg accggcgacg ccaacaagta ttacgtgctg cgccatcggg
     3961 aatgggacta cgaccagttc gccacggcgg cgatcgactc ggccgatccg ctgtggctga
     4021 cgccgccggc cgcgctggca gtcgatctgt gcaatggcta tgacgcgcgc ttcctcctct
     4081 ccctcggccc gttcaccctc gctcccgatt ccagcttccg cgtgatcttc tccatgttca
     4141 cggcggactc catccacact gtccccggga acctggccca tcttcccgct gacccggccg
     4201 cgtaccgtga caacctgggt ctcgagcggc tc
//
LOCUS       DAOOKS010000064         4212 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_25377_length_4212_cov_3.153236, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000064 DAOOKS010000000
VERSION     DAOOKS010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4212
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25377_length_4212_cov_3.153236"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..950
                     /locus_tag="PK112_01740"
     CDS             <1..950
                     /locus_tag="PK112_01740"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPC10802.1"
                     /translation="MVHPFAAAGPFLAGLLGALYRPGCRLQAIGHVTPEVELAADRAE
                     VEVIEHIGPSPFSGSLQTALQGTVLPHDILCAANPNRVTGANYSLADVQRMAETVPEG
                     AVLVDEHYHDYFGITALPLLSDCPNVIILRSFAPGGALGGDEPGYALACPRTIARLRA
                     ALPESVLPAAEARLLIDRWPGGEALAARLREGHQESLRLAEGLTRLGVQCRLSATDFI
                     LLRVADPARVGNHLSRARIAIDNLDGYPAMQHYLRYRVQSPLSNDRLLQAFQAMPPES
                     FRMKTIDGRVMTMRLSAQAARKGAGRSGNLLMAALRDTE"
     gene            1139..2548
                     /locus_tag="PK112_01745"
     CDS             1139..2548
                     /locus_tag="PK112_01745"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HPC10803.1"
                     /translation="MHLTHRRRQFLLRLAGSPGSHAGAVAALCATLMAAGSSLGLLVE
                     IAAAVTLARLLAFVHPHPRPVLDAPPVRKYAGAALAAEARFAVLVLAAAYLLDWPLPR
                     LAVPVFLGLNFVTQLGLLVGARLILRRLARSPLPDRLRRARNRLLVVGTGARAQALAD
                     MILNAPELDAAIDGFLDYRRTGFWRYRDIPLLGHPDLLTPLAARVQVDAVILAVEPED
                     LSRTAPLLTAAEQLGVAVALLPDVYPTTIARMVPATLNGSPAVVWRRVPESPAALFVK
                     NALDRLGGLVGLILTLPLMLVTALAIKLESPGPVFFAQRRAGANGRPFSLYKFRTMRA
                     DAEKHKESLRGLNEMSGPVFKIRNDPRLTRVGRFLRRFSIDEIPQFYNVLRGDMSLVG
                     PRPPLPSEVARFEPWQRRKLSVKPGLTCIWQVSGRNKIDFEDWMKLDLQYIDNWSLWL
                     DAKILARTVPTVLKGDGAV"
     gene            2551..4011
                     /locus_tag="PK112_01750"
     CDS             2551..4011
                     /locus_tag="PK112_01750"
                     /inference="COORDINATES: protein motif:HMM:NF025418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HPC10804.1"
                     /translation="MKALFRILALLAVLLLLPWAIKAAGVLPLGSPEFEFLYDRLERA
                     DALTLDTADRQLGPYPLALAREALGPFAWLDRLDRSDLRVEGRLGEDFRSAKGHPTVG
                     YESFSGALAARPHDRIFVYGSFRLDEALADDPAYTGKKWRGLAGDIDEAFVHLSAGAF
                     TAALGRYASFWGPRRSLVLAPHAALDGFGYAYRWGRLVLSYRLARLDSLDPDADSAVP
                     VENRYFAGHRLDIHLSRRLRIGFFETAVFGGAGRQVELYYLNPILFYHASQLNDGVDD
                     NTFLGVDFAYEPGRGVQLYGQLLVDDYQVDRASQSDQEPNEIGLLAGAYVAEFLPRLD
                     LRAEYTRVTNRTFNQSHPRNRYTFESELLGAALGNDYDLLDLLIRRWCGEDLAAGVRL
                     AYRRQGEGRVNAAWDTPWLEVEGDYREPFPTGVVEKTFTAALQLRGLLFGHIFVDASF
                     GADRVADFGHVRGDRRTISFAAIKLITFASQALDID"
BASE COUNT          590 a         1516 c         1375 g          731 t
ORIGIN      
        1 cgatggtgca tccgttcgcg gcggccggcc cgtttctcgc cggcttgctg ggggcgctct
       61 atcggccggg ctgccgcctc caggcgatcg gccatgtgac cccggaggtc gagctggccg
      121 ccgaccgggc cgaggtcgag gtgatcgaac acatcggccc atcgcccttc tccggcagcc
      181 tgcagaccgc tctgcagggg acggtgctcc cccacgacat cctctgcgcg gccaacccga
      241 accgcgtcac cggcgcgaac tactcgctgg ccgatgtgca gcggatggcg gagacggtcc
      301 cggagggggc cgtgctggtc gatgagcact accacgatta cttcggcatc acggcgctcc
      361 cgctgctgag cgactgtccc aatgtgatca ttctccgctc gtttgcgccc gggggcgcac
      421 tcggcggcga cgagcccgga tacgccctcg cctgcccccg cacgatcgcc cgccttcgcg
      481 ccgctctccc cgagagcgtg ctgccggccg ccgaggcccg tctgctgatc gaccggtggc
      541 ccggaggaga agcgctggcc gcgcgcctgc gcgaggggca tcaggaatcg ctccgcctgg
      601 ccgagggact cacccgcctc ggcgtgcagt gccgcctcag cgccaccgat ttcatcctcc
      661 tccgcgtcgc cgacccggcg cgggtcggta atcacctgag ccgcgcgcgg atcgcgatcg
      721 acaatctcga cggctacccg gcgatgcagc actacctgcg ctaccgggtg cagtcgccgt
      781 tgtccaacga ccgcctgctg caggccttcc aagcgatgcc cccggagtcg ttccgcatga
      841 agacgatcga cgggcgggtg atgacgatgc gcctgagcgc ccaggcggcc cgcaagggcg
      901 cgggccgcag cggcaacctc ctcatggccg ccctgcgcga caccgagtga accgctcagt
      961 cgccgtatga aaaaacttcg gctgttcata gctgcacctc cggaacaccg accgaagcgc
     1021 ccgtcgcttc ggtcgggatc ctcgcgcgac tccctgcgga cccgccggga ggacggccga
     1081 gacgagaggc gaccgacgcg cggtgcgcgg gaagatcatc attggagagc ctgatcgtat
     1141 gcacctcacc caccgtcgcc gtcagttcct cctccgcctg gccgggtctc cggggtccca
     1201 tgccggggct gtcgccgcgc tctgcgcgac actcatggcg gccgggtcgt cgctggggct
     1261 gctggtcgaa atcgccgccg ccgtcaccct agcgcggctg ctcgcgttcg tccacccgca
     1321 cccgcgtccg gtgctcgacg ctccgccggt gcggaaatac gccggcgcgg cgctcgccgc
     1381 cgaggcgcgc tttgccgtgc tcgtgctggc ggcggcctac ctgctggact ggccgctgcc
     1441 ccgcctggcg gtgccggtct ttctgggact gaatttcgtg acccagcttg gtctgttggt
     1501 cggggcgcgg ctgattctcc ggcggctggc ccgctcgccg ctgcccgacc gcctccgccg
     1561 cgcccgaaac cgcctcctcg tggtcggcac cggggcgcgc gcccaggcgc tggccgacat
     1621 gattctcaac gcccccgagc ttgatgcggc gatcgacgga ttcctcgact accgccgcac
     1681 cggcttctgg cgctaccgcg acattcccct gctgggccac ccggacctgc tcacgccgct
     1741 ggccgcgcgg gtccaggtcg acgccgtcat tctcgccgtg gaaccggagg acctctcccg
     1801 tacggcgccg ctgctgacgg cggccgaaca gctcggggtg gcggtggcgc tgctgcccga
     1861 tgtgtacccg acgacgatcg cgcgcatggt gccggcgacg ctcaacggtt cgccggcggt
     1921 ggtctggcgg cgcgtgccgg agagtccggc cgccctgttc gtcaagaacg cgctcgaccg
     1981 cctgggcggt ctggtcggcc tcatcctcac gctgccgctc atgctggtga ccgcgctcgc
     2041 catcaagctg gagtcccccg ggccggtgtt tttcgcgcag cggcgcgccg gcgccaacgg
     2101 ccggcccttt tcgctctaca agttccgcac gatgcgagcc gatgccgaga agcacaagga
     2161 gtcgctacgg gggctgaacg agatgtccgg gccggtcttc aaaatccgca acgatccgcg
     2221 cctgacgcgg gtggggcggt tcctgcgccg gttctcgatc gacgagatcc cgcagttcta
     2281 caacgtgctg cgcggcgaca tgtcgctggt cggcccgcgg ccgccgctcc cctccgaggt
     2341 ggcccgcttt gaaccctggc agcgccgcaa gctctcggtc aaacccggcc tcacctgcat
     2401 ctggcaggtg agcggccgca acaaaatcga tttcgaggac tggatgaagc tggatctcca
     2461 gtacatcgac aactggtcgc tctggctgga cgccaagatc ctcgcccgca ccgtccccac
     2521 ggtcctcaaa ggcgacggcg cggtttgacg atgaaggcgc ttttccgcat actcgcgctg
     2581 ctggcggtgt tgctgctgct gccctgggcg atcaaggccg ccggcgtgct cccgctgggc
     2641 agcccggagt tcgaatttct ctatgaccgc ctcgaacgcg ccgatgccct cacgctcgac
     2701 acggccgacc gccagcttgg tccatacccg ctcgcccttg cccgcgaggc gctcggtccg
     2761 ttcgcctggc tggaccggct cgaccgctcg gacctgcgcg tggaaggccg cctcggtgag
     2821 gatttccgga gcgcgaaggg tcacccgaca gtcggctatg agtcgttctc cggcgctctg
     2881 gccgcgcgcc cgcacgacag gatttttgtc tacggcagct tccgcctcga cgaggcgctt
     2941 gcggatgatc cggcgtacac cggcaagaag tggcgcggtc tggcgggcga catcgacgag
     3001 gccttcgttc acctgagcgc cggggccttc accgccgcgc tcgggcggta cgcctcgttc
     3061 tgggggccgc gccggtcgct cgtgctggcc ccgcacgcgg cgctcgatgg tttcgggtac
     3121 gcctaccgct ggggacggct ggtgctctcc taccggctcg cccggctcga cagccttgat
     3181 ccggacgccg acagcgctgt gccggtcgag aaccgctatt tcgcggggca tcggctcgac
     3241 atccacctga gccgccgcct gcggatcgga ttcttcgaaa cggcggtgtt cgggggcgcc
     3301 ggacggcagg tggaattgta ctacctcaac ccgattctgt tctatcacgc ctcgcagctc
     3361 aacgacggcg tggacgacaa caccttcctc ggtgtcgact tcgcctacga acccggccgc
     3421 ggagtgcagc tctacgggca actgctggtc gatgactacc aggtcgaccg ggcctcgcag
     3481 agcgaccagg aaccgaacga gatcggcctg ttggcgggcg cgtatgtcgc cgagttcctg
     3541 ccccgactcg acctgcgggc ggagtacacg cgcgtcacca accgcacctt caaccagagc
     3601 cacccccgca accggtacac gttcgagagc gaactgctcg gggcggcgct ggggaatgac
     3661 tacgatcttc tcgatctgct catccggcgc tggtgcgggg aggatctggc tgccggcgtg
     3721 cggctggcct accgccgcca gggggaaggc cgcgtcaacg ccgcctggga caccccgtgg
     3781 cttgaggttg aaggggatta ccgcgagccg ttcccaaccg gcgtggtgga gaagacattc
     3841 acggcggcgc tgcagctgcg gggactgctt ttcgggcaca tcttcgtcga tgcttctttc
     3901 ggggcggacc gggtggccga tttcggccac gtgcgcggcg accgccggac gatctccttt
     3961 gcggccatca agctgatcac atttgcctcc caggctcttg acatcgatta aacgccgcct
     4021 atactttctc cgaacaacag cctttcatct tttggggagt gaaccgccgt gagcacccgg
     4081 ccgcaccgct gtcagtcgcg ttttgcgttt acggataccg ctaccgccgt cctgatcatt
     4141 atattcgccc tcgcgttcat tgacggagat cccccggcat atttcctgaa tgcgtcggtc
     4201 acatccaggg ca
//
LOCUS       DAOOKS010000065         4192 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_25514_length_4192_cov_2.913947, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000065 DAOOKS010000000
VERSION     DAOOKS010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25514_length_4192_cov_2.913947"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="PK112_01755"
     CDS             complement(<1..771)
                     /locus_tag="PK112_01755"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10805.1"
                     /translation="MHLVDYAIIVAYLAVLLYLGLRNPLTGKNSTGELILGGRLLALP
                     AFVASLVSTWYGGILGVGEYSYRHGLSNWLVFGLPYYLAAALFAVLLARRARESLVLT
                     IPDRLAQAYDNRTAIAGAAIIYLMTVPSAYILMTGVLCEYLFGWPFWLGVVVGTFFSI
                     VYVYTGGFVSMVRTDMLQFVLMFLGFIVMLGAAVARYGGWSFLRSAVPADHFTWHGGN
                     APFYVAIWYVIALATLIEPAFYQRCYAAPSVRVARSSQR"
     gene            complement(788..3268)
                     /locus_tag="PK112_01760"
     CDS             complement(788..3268)
                     /locus_tag="PK112_01760"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPC10806.1"
                     /translation="MRCAVLITIMLAPFAAAVEAAPLSGMVQNPDGEPLAAVSLTTNV
                     AGLGTSTDAAGAFTLECPGDVRWVTFSSVGYQPRQYPVDAVPARVVLAYEYSPGGDVV
                     VRGDRAQPGLSPIAFTDYSRKDIARDYSIGEFPLLLQATPNLYTYTDGGGALGYSSVR
                     IRGFDDKRIAASLNGVPLNDPEDQAAYFVDLPDFAANITDIQVQRGVGNALYGAASFG
                     GSLNIVTGALSRDRRVTVTSGYGEYTSGGDRVSDLAKSSIEYVSGLIDGRWLFTGRFS
                     DQRSGGYRRHSWYDGWSYYLSLARLDPRMTTELYLYGGPIRMHLAYYGVDRATLAVDR
                     RANPLTYRNETDNFNQPHFHLHNTYQLSERATLATTLYYIQGEGYYEQLKQLEDPDDF
                     AVYGLDPGELAVDQPRLVRQKWVDKRQWGWNPRLDLAHARGRHSIGGSAYYFTSDHWG
                     QVVWVEGLEDRSYGPRQRYYRYEGRKVEANLFAQEYCRLTDRLAGEATAMLRYQRYDF
                     DQTRMYSFRGFRYDVDWLFFSPRLGFTYTLAPEIALHTAFAVSSRTPTDEDIYEADDP
                     FALPSLDIRSVSADSARWEFGDPTAKAERVYDFELGGGYRSDRYAVELALYWMEFRDE
                     IIPEGAVDASGRRRTINADRSVHAGLELSGTARPTEALALSGNFAWAYNRIRDYTASL
                     DLYDTDWNYAGQYRFDLADKTVPGFPAYLGNVLLDYRYRGLRAVWRHQFVGAQELDLA
                     NTDSLRIEPFQLASVSLSYRFSDFWHLGALTLTALVNNVFDRRYEAGGYGWNYAVAGD
                     GGTDVLGGAEYFAAPERWYYGEVKLEIF"
     regulatory      complement(3353..3450)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3571..>4192
                     /locus_tag="PK112_01765"
     CDS             3571..>4192
                     /locus_tag="PK112_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006302064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPC10807.1"
                     /translation="MPAVTDKRSPEEIRKAVREHYGTAITKGTGGCCGPQPQALDPAA
                     ARRYEELAGYRAGETEGLPDGVTSFGCGNPVNFAEIKPGQTVLDLGSGAGLDLILAAK
                     RVGPAGRVIGLDMTPEMIATCRRNLAAAGIANAEVVPGEMEHMPLPDASVDWIISNCV
                     INLSPDKEKVFAEAFRVLRPGGRVLIADIVTNSLPEEYRDDLSAWVG"
BASE COUNT          789 a         1303 c         1457 g          643 t
ORIGIN      
        1 acgctgcgac gagcgggcga cccgcacgct cggcgcagcg tagcagcgct ggtagaacgc
       61 tggctcaatc agggtggcca gggcgatcac ataccagatc gccacgtaga acggggcgtt
      121 gccgccgtgc caggtgaaat ggtccgccgg cacggccgag cgcagaaacg accacccgcc
      181 gtagcgcgcg acggctgctc cgagcatgac aatgaacccg aggaacatga gtacgaactg
      241 cagcatatcg gtgcgcacca tcgagacgaa cccgcccgtg tacacgtaca cgatcgagaa
      301 gaaggtgccg accacgacgc ccagccagaa cggccagccg aacagatact cgcacagaac
      361 gccggtcata agaatgtagg cggaggggac agtcatgagg tagatgatgg ctgccccggc
      421 gatggcggtg cggttgtcgt acgcctgggc gaggcggtcg gggatagtca gcacgagcga
      481 ttcgcgcgcg cggcgggcca ggagcaccgc gaaaagcgcc gcggcgagat agtagggaag
      541 cccgaaaaca agccagttgg acagcccgtg acggtagctg tactccccga ccccgaggat
      601 cccgccgtac caggtcgaga ccaggctggc gacaaaggcc gggagggcga gcaggcggcc
      661 cccgaggatc agctcgcccg tgctgttttt ccccgtcagc gggttgcgca ggccgaggta
      721 caacagcacg gccaggtagg cgacaatgat ggcgtaatca acgaggtgca tacccgccgc
      781 cggccggtca gaagatctcc agcttcactt ctccatagta ccatcgttcg ggcgcggcga
      841 aatactccgc gccgccgagc acgtccgtcc cgccatcccc tgcaaccgcg tagttccacc
      901 cgtagccgcc cgcctcatag cgccgatcga acacgttgtt gaccagggcc gtgagagtga
      961 gagcgcccag atgccaaaaa tcggagaacc ggtaggagag actcaccgag gcgagctgga
     1021 acggctcgat tcgcagactg tcggtgttgg cgaggtcgag ctcctgggcg ccgacaaact
     1081 ggtgccgcca gaccgcccgg agaccgcgat agcggtaatc cagaaggacg ttgccgaggt
     1141 acgccgggaa accgggcacg gttttgtcgg cgagatcgaa ccggtattgc ccggcgtagt
     1201 tccagtcggt gtcgtagagg tcgagcgatg ccgtgtagtc gcggatccgg ttgtaggccc
     1261 aggcgaaatt gcccgacagg gccagggcct ccgtaggccg ggccgtgccg ctgagctcga
     1321 ggccggcgtg gaccgagcgg tcggcgttga tggtccggcg ccggccggag gcgtcaaccg
     1381 ctccctccgg gatgatctcg tcgcggaact ccatccagta cagggcgagc tcgaccgcgt
     1441 accggtcgga acggtacccg ccgcccagct cgaagtcgta cacccgctcg gccttggccg
     1501 tcggatcgcc gaactcccag cgcgcggagt cggcgctcac ggaacggatg tccagcgagg
     1561 ggagggcgaa gggatcatcg gcctcgtaga tatcctcgtc ggtcggagtg cgcgaagaca
     1621 cggcgaaagc cgtatggagc gcgatttccg gggcgagcgt gtaggtgaaa ccgaggcggg
     1681 gggagaagaa cagccagtcg acgtcgtagc gaaaaccccg gaacgagtac atgcgggtct
     1741 gatcgaagtc gtaccgctgg taccggagca tggccgtggc ttcgccggcc aagcggtcgg
     1801 tgaggcggca gtattcctgg gcgaacaggt tggcctcgac tttcctcccc tcatacctgt
     1861 agtaccgctg gcgcgggccg taggaccggt cttcgagacc ttccacccag accacctgtc
     1921 cccagtgatc ggaggtgaag tagtaggcgg aaccgccgat gctgtggcgg ccgcgggcat
     1981 gggcgagatc aagccgcggg ttccaccccc actggcgttt gtccacccac ttctgccgca
     2041 cgagtctcgg ctgatccacc gcaagctccc ccggatcaag accgtacacg gcgaagtcgt
     2101 ccggatcctc cagttgtttg agttgctcgt agtagccttc cccctggatg tagtacaggg
     2161 tggtggcgag tgtggcgcgc tcgctgagct ggtaggtgtt gtggaggtgg aagtggggct
     2221 ggttgaagtt gtcggtctcg ttgcggtagg tcagcgggtt ggcgcggcgg tcgacagcaa
     2281 gcgtcgcccg atcgaccccg tagtaggcca ggtgcatgcg gatcggtccc ccgtagaggt
     2341 acagctcggt cgtcatgcgc gggtcgagcc gggccagcga caggtagtag gaccagccgt
     2401 cgtaccagct gtggcggcgg tagccgccgg agcgctggtc ggagaagcgc ccggtaaaca
     2461 gccagcggcc gtcgatcagc cccgacacgt actcgatgct cgacttggca aggtcgctga
     2521 cccggtcgcc acccgaggtg tactcgccgt agccggaggt cacggtgacg cggcggtcgc
     2581 ggctgagggc gccggtgacg atgttgagtg aaccgccgaa cgacgccgcg ccgtagagcg
     2641 cgttgccgac cccgcgctgg acctggatgt ccgtgatgtt ggcggcgaag tcgggcaggt
     2701 cgacgaaata ggcggcctgg tcctccgggt cgttcagcgg gacgccgttg agagaggcgg
     2761 cgatccgctt gtcgtcgaag ccccggatac gcaccgagct gtagcccagg gcgcccccgc
     2821 cgtcggtgta cgtgtagagg ttcggggtcg cctgcaggag cagcgggaac tcgccgatgc
     2881 tgtagtcgcg ggcgatgtct ttccgcgagt agtcggtgaa tgcgatcgga gacagacccg
     2941 gctgggcgcg gtcgccgcgc acgacgacat ccccccccgg cgagtactcg tacgccagca
     3001 ccacccgcgc cggcaccgcg tcgaccgggt actgccgggg ctgatagccg accgaggaga
     3061 aggtcaccca gcggacgtcg cccggacact cgagcgtgaa agcgccggcg gcgtcggtgc
     3121 tcgttcccag gccggcgacg ttggtggtca gggagacggc ggccagcggc tcgccgtcgg
     3181 gattctgcac catgccggac agcggcgcgg cctccaccgc cgcggcaaac ggcgccagca
     3241 tgatcgtgat gaggacagca catctcatga caacaacctc gcgtcgatgt gacgattcag
     3301 caaggttccg tcctgagcgt cataggtagc ccggaaaagg ccaggggatg cgatgcgaac
     3361 atctccgttt ccctacgccg gtattacccg gatcaggtcg agggggtatg atctcaggcg
     3421 gtcgttctta cgccaccccc aacggaacgg tcttttcagt cgctctccat atattgcgga
     3481 aacggacccg gcgcaagcga tttttcgcct ggaccgggga aactcccgcg ggttataata
     3541 ggcaaagacg agcgaaggcc tctacgaaag gtgccggccg tgacagacaa acgatctccc
     3601 gaggaaatcc gcaaagcggt ccgcgaacac tatgggacgg caatcacgaa agggaccggc
     3661 ggttgctgcg gcccgcagcc gcaagcgctt gacccggcgg cggcccggcg gtacgaggaa
     3721 ctggccggct accgtgcggg cgagaccgaa gggctgcccg acggggtgac ctcgttcggg
     3781 tgcggcaacc cggtcaattt tgccgagatc aagccggggc agaccgtgct cgacctgggc
     3841 agcggcgcgg gactcgatct catcctggcg gcgaaaagag tcgggcccgc cgggcgcgtg
     3901 atcggcctgg atatgacgcc cgaaatgatc gcgacctgcc ggcggaatct cgccgccgcc
     3961 gggatcgcca atgccgaagt cgtgcccggc gaaatggagc acatgcccct tccggacgcc
     4021 tccgtcgact ggatcatctc caactgcgtg atcaacctct cgccggacaa agagaaagtg
     4081 ttcgccgagg ccttccgcgt gctcaggccg ggcggacgtg tgctgatcgc cgatatcgtc
     4141 accaacagtt tgcccgagga gtaccgggac gacctctcgg cctgggtggg gt
//
LOCUS       DAOOKS010000066         4189 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_25538_length_4189_cov_3.268989, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000066 DAOOKS010000000
VERSION     DAOOKS010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4189
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25538_length_4189_cov_3.268989"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="PK112_01770"
     CDS             complement(<1..531)
                     /locus_tag="PK112_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding family 9 domain-containing
                     protein"
                     /protein_id="HPC10808.1"
                     /translation="MIHGRREESPRFSMRMCALCFWMCLAAMAAAAPEAPPRPTLAIA
                     RAAGPIVIDGDLSEGAWRTAANATDFTQFLPIDRVRPKSATSAYVTYDDRMLYVGFVC
                     DDDPAQVRATLTDRDRMYSDDFVGIILDTYGDAAWAYEFYVNPLGVQGDMRWLASGDE
                     DTRFDVVYYSAARVTDS"
     gene            complement(669..1004)
                     /locus_tag="PK112_01775"
     CDS             complement(669..1004)
                     /locus_tag="PK112_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPC10809.1"
                     /translation="MKFTDTLEGDLVIIEISGKIMGGEETTMFHGRIHEYIKLNKKNF
                     LINLKKVEWMNSVGLGMLISALTTVRNAGGRLVLANIDKIESILTITRLISVFDHYDS
                     VAEARKSFA"
     gene            complement(1593..1850)
                     /locus_tag="PK112_01780"
     CDS             complement(1593..1850)
                     /locus_tag="PK112_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10810.1"
                     /translation="MGFDADVAGLVGEYAGIPGGNHRAGPGSPASELAALADWLVEHH
                     AWTPSAARHLVELVARNGTFVLRNALALSIVLGVEDGELGL"
     gene            complement(2666..3742)
                     /gene="recA"
                     /locus_tag="PK112_01785"
     CDS             complement(2666..3742)
                     /gene="recA"
                     /locus_tag="PK112_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HPC10811.1"
                     /translation="MKGKDKAAGSQAPDRKKALEAALIQIERSFGKGAIMRLGDESVQ
                     KVQVISTGSIGVDHALGVGGIPRGRVVEIFGPEASGKTTMALHMIAEAQKAGGVAAFV
                     DAEHALDATYARNLGVDTDNLLVSQPDTGEQALDITETLVRSGAVDIIVVDSVAALTP
                     RSEIEGEMGDAHMGLQARLMSQALRKLTAIISKSKTSVVFINQIRMKIGVMFGNPETT
                     TGGNALKFYASVRLDIRRIASIKENQEVIGSRTRVRVVKNKVAPPFREAEFDIIYGKG
                     VSREGELLDIAAAKGIIEKSGAWFSYGGDRLGQGRENAKQFLADNPALFDEVYKKVQV
                     ELGISGGIGSSSGAAEEEPAEVEG"
BASE COUNT          726 a         1479 c         1187 g          797 t
ORIGIN      
        1 ggaatcggtg acgcgggcgg cgctgtaata gacgacgtcg aagcgggtgt cctcatcgcc
       61 cgaggccagc cagcgcatat ccccctgcac gccgagcggg ttgacgtaga actcgtaggc
      121 ccacgccgca tccccgtagg tatcgaggat gatccccacg aaatcgtcgc tgtacatccg
      181 gtcacggtcg gtcagggtgg cccgcacctg cgccggatcg tcgtcgcaca cgaaacccac
      241 gtacagcatc cggtcgtcgt aggtgacata cgccgaggtg gcggatttcg gccgcacccg
      301 gtcgatcggc aggaactggg taaaatcggt ggcgttcgcg gccgtccgcc acgctccctc
      361 ggacaggtcg ccgtcgatta cgatcggccc cgcggcgcgc gcgatcgcca gggtcggccg
      421 cgggggcgcc tccggcgcgg ccgccgccat cgcggccaga cacatccaga agcagagcgc
      481 gcacattcgc atgctgaacc tcggcgactc ctcgcggcga ccgtggatca taccgtcgcc
      541 gttaccttcc gtatacggcg gccggggggc gccggttgcc ggcccgccgg tcgctcacgg
      601 agcaccggac aacaaaaggg cctcgcacgg caaggccctc tccaccgtcg accatggcgc
      661 cggcgctctc aggcaaatga tttcctggcc tcggccacgc tgtcgtagtg gtcgaacacc
      721 gagatcagcc gggtgatcgt caggatcgat tcgatcttat cgatgttcgc cagcaccagc
      781 cgcccgcccg cgttccggac cgtcgtcaac gccgaaatca gcatccccag gcccaccgag
      841 ttcatccact cgaccttctt caggttaatc aggaaattct tcttgttcag cttgatgtac
      901 tcgtggattc tcccgtggaa catggtcgtc tcttccccgc ccatgatttt gccggaaatt
      961 tcgatgatga ccagatcccc ttcgagcgtg tcggtaaact tcatagcccg atcctcagtc
     1021 tgtatatagt ggtttgctgt cgtccgtcac ctatcggccg ctctcgtccg ggtcctgggg
     1081 caggtcggag agaggcacgc gatcaccctg ccggaactgc atccccggtc cgatcaaaat
     1141 accggccgcc caaccaaacg caagcggttt ttgggcgccg cccgcccgct cgttttccgc
     1201 ttgcgcaaac gggccgggca ggttatacta aggcgattgt aaatggcagc atcactctac
     1261 gccacgaggt cctttcgatc tttcgctaac ggactgtcac gtgcggttct gaacgtcgac
     1321 ggggaatagc ttatgcgtcc gttacgctcc cgccgtaatc ccggcgctgt cgcccggccg
     1381 ccgagcggtg tttcgccgcg gccgcgcaag cccgaccgtt ccggttgacc cgccgacatc
     1441 cggtcgccgg cccgtgagcc cgtgcggcat tcggggcgag gtcgggacgg cgatggagga
     1501 aaattcccgg ccgctgagcc cggtcgggaa cgcaacgcaa aggccgctcc gaaggatccg
     1561 gagcggcctg ctttatgctt gcgcctttcc ggctagaggc ccaactcgcc gtcctcaaca
     1621 cccaggacga tcgagagcgc cagagcgttg cgaaggacga acgtgccgtt ccgggcgacg
     1681 agttcgacga gatggcgcgc ggccgaaggt gtccatgcat ggtgctccac cagccaatcg
     1741 gcgagtgcgg caagctcaga cgcaggcgag cccggaccgg ctcgatggtt ccctcccgga
     1801 atgcccgcgt actctcccac cagaccggct acgtcggcat caaaccccat tgctctcctc
     1861 ctcactgtcc tcggaacact ttctggcgat gccactccaa tcgcaccggt gacggggcaa
     1921 atctccgcgg cggggcgatt gggcggccgt gataaacccc gacgagctgc cgccctcggc
     1981 gatcgagccc ctcccggagg cttgcggcaa gaacgacgcg gagatcagtg tcgacagtca
     2041 tgaagccccg gtcaaagagc ctgtcaaagg tcgcgctgag acagagcccg ttgtggggat
     2101 cggcacgtcc ctcttcagcg agactccacg gaactatgtg gctcgcgatt agacactccc
     2161 gaattctcag tccggtaacg cagcaccgct catcataggc gctcaggacg gcctcgcgga
     2221 aaaagtcctg gccgaccctc acctttctgg aggccagagt ctctgtcggc ccgctgcgca
     2281 atgtttccgc gctctgcccc acctcttgga tccgtcgctc gcggagttga cttgcctgca
     2341 ggacgagttc actccaattg gagtggaact ctcgccagac cgctcgatcc aaatccccgg
     2401 tgtgcggcag gcccccgatc tggcgtttcc gaagggccgg gtcaagcgat ccaaaattcc
     2461 ccagtttccg ggcgacgccg gccggtgacc gccccagcaa ccgcgctaat tcaacgacga
     2521 ggggattgcc cgctttcgtg ttcgcaaacg ggatgcggca atagagatcg aaagcaagaa
     2581 tcaactcctc ccggtcccag ggagccccat accgtggagg tctctggtcc tctcccatgc
     2641 ctgtgcccgc tccggcgttt ccccctcagc cctctacttc cgccggctcc tcctccgccg
     2701 cccccgagga actcccgatc ccgcccgaaa tgcccagctc cacctgcacc ttcttataga
     2761 cctcgtcgaa cagcgccggg ttgtccgcca aaaactgctt cgcgttttcc cttccctggc
     2821 cgagccggtc gccgccgtag gagaaccagg cgccggattt ctcgatgatt cctttggcgg
     2881 ccgcgatatc gagcagctcc ccttcgcgcg acaccccctt gccgtaaatg atgtcgaact
     2941 ccgcctcgcg gaacggcggc gccaccttgt tcttgactac ccgcacgcgc gtgcgcgacc
     3001 cgatcacctc ctggttctcc ttgatcgagg cgatccgccg gatgtcgagc cgcaccgagg
     3061 cgtagaattt cagcgcgttc ccgcccgtcg tcgtctcggg attgccgaac atgaccccga
     3121 ttttcatgcg gatctggttg atgaagacga cgctcgtttt ggatttgctg atgatcgcgg
     3181 tgagtttgcg cagcgcctgc gacatgagcc gcgcctgcag ccccatgtgc gcatccccca
     3241 tctccccctc gatctccgag cgcggcgtga gcgcggccac cgaatcgacc acgatgatat
     3301 ccacggcgcc cgaccgcacg agcgtctcgg tgatgtcgag cgcctgctcg cccgtgtccg
     3361 gctgcgagac cagcaggttg tcggtatcca ccccgagatt gcgcgcgtac gtggcatcga
     3421 gcgcgtgctc ggcatcgaca aaagccgcca ccccgcccgc tttctgcgcc tcggcgatca
     3481 tgtgcagcgc catggtcgtc tttcccgacg cctccgggcc gaatatctcg accacccggc
     3541 cgcgcgggat ccccccgacc cccagcgcat gatcgacccc gatcgagccg gtcgagatca
     3601 cctgcacctt ctgcaccgac tcgtccccca gccgcatgat cgcccctttg ccgaacgacc
     3661 gttcaatctg aatcaacgcc gcctcgagcg ctttcttacg gtccggcgcc tgcgatcccg
     3721 ccgccttgtc tttgcctttc attaccatcc ggctccttct ctgcctgcct aaatgtgcgc
     3781 ttctcccgcc attatagaac gaacgttttc ggaaatatat cacatctttc actctccgga
     3841 aaccgaattg tccgcccctg gccgtctcca ccgcagcggt ttcaccggtc cggtccaggg
     3901 ggcgatcggt cgttctgcgg aagtttgcat gccctgcttc acaccccgcc cggaactttc
     3961 cgacagcact ccttccgcac cattcaacct gttgtcggac aataaaatac aagagggcgc
     4021 ctgctttcga tttcccccgc cggggcatac ttgttgctct aatcccccgg catgaccacc
     4081 acccgggctc tgtcggcccg accctcggtc gggcgaagga accggacgac tgcgaacccg
     4141 tgaattgact ggaaatgaaa acaacagttt agcgcttgtc gtcgcggcc
//
LOCUS       DAOOKS010000067         4181 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_25602_length_4181_cov_3.879060, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000067 DAOOKS010000000
VERSION     DAOOKS010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4181
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25602_length_4181_cov_3.879060"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..1662)
                     /gene="ctaD"
                     /locus_tag="PK112_01790"
     CDS             complement(19..1662)
                     /gene="ctaD"
                     /locus_tag="PK112_01790"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HPC10812.1"
                     /translation="MAEATAVNYLNATRGWKSWLLTLDHKRIAILYMAAIAVFFFVGG
                     VLAMLIRLELLHPGRDLVGADTYNKLFTLHGAIMIFLFIIPGVPAILGNFLLPLMLGA
                     RDVAFPRLNLASWYIFMLGSLFALYSIVAGAADTGWTFYTPYSTQTDTSVISMTLGAF
                     ILGFSSIFTGLNFIVTIHKLRLPGMTWYRMPLFVWSMYATAIIQVLATPVLGITLLLL
                     VIERVAGIGIFDPAMGGDPVLYQHFFWFYSHPAVYIMILPGMGIVSELITAHAHKKIF
                     GYKAVAYSSLGIAFVSFLVWGHHMFVSGQSELAAMLFSFLTFFVGIPSGIKVFNWVTT
                     LYRGSIKLTTPMLYALSFLFLFSIGGFTGIMLGALAVDVHVHDTYFIVAHFHYVMMGG
                     TVIAFLGGLHHWWPKMFGRMYNEPWAKVACALIFVGFNVTFGSQFLLGLHGMPRRYYN
                     YLDQYQPLHAFSTFGSWILGAGFVIMAIYLIHSLARGRRAGSNPWRALTLEWETPSPP
                     PTENFPFTPSLAHGPYDYDRVIPVNVEPGGRAVRPEVVS"
     gene            complement(1667..2635)
                     /gene="coxB"
                     /locus_tag="PK112_01795"
     CDS             complement(1667..2635)
                     /gene="coxB"
                     /locus_tag="PK112_01795"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HPC10813.1"
                     /translation="MDTTGTLFFPPAGSTLAGEVDSLFYFIFWASAVLFAIVIGLTVF
                     FIIRYRRRRPTGTTYGRDHNLALEITWTAVPTVLILIVFVWGFRAYMRMTIVPRDALE
                     VKVTAQKWFWTFDYPNGANSVNELVAPVDRPVKLLMSSRDVIHSLFVPDFRMKMDVLP
                     NRYSVTWFEATRPGTFNLFCTEYCGTGHSEMLAKVKVVSEREFAEFLASTGGPGAGES
                     PADYGRRLYTSRACNTCHTLDGKPSVGPTWQGLWGRREALQTGDFVTVDENYIRESVL
                     EPQARVTAGFQPVMPTYQGILTDRDIDALIAFIKSLDTTNSEAAGE"
     gene            complement(2645..3475)
                     /locus_tag="PK112_01800"
     CDS             complement(2645..3475)
                     /locus_tag="PK112_01800"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HPC10814.1"
                     /translation="MQRVLRNIGFLALLAAAGGAAGQTVRTDPPELRGIDVHERLGDT
                     IPRGLGLVDEAGREVALGDYFGGDRPVLLVLGYYECPMLCNLVFNGLTEALPRVGLDL
                     GRQYRVVTVSIDPGETPDLAAAKKASYLAQLGPAAPPASWAFLTGDSAAIAQLADAVG
                     FRYYYDESRDEYAHPAVVHVLSPEGVVSRYLHGIQYNPRDLKLALVEAADGKIGSTLD
                     RVILYCYHYDPGAGGYVVLAQNVMLVGGLLTVLGLGGFLLVLWLRESYRTRHRPAPAG
                     "
     gene            complement(3592..3939)
                     /locus_tag="PK112_01805"
     CDS             complement(3592..3939)
                     /locus_tag="PK112_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10815.1"
                     /translation="MTDTENKPAPGREEKAGYDTSDVNVTKALVVGVLLTLLVIAFAV
                     VLNEYFIAVTEEISYEQQRAPESKALRELRAREVEQLESYGVQDGAAGSYRIPIERAM
                     ELQAQEAFTAKAR"
     gene            complement(3949..>4181)
                     /locus_tag="PK112_01810"
     CDS             complement(3949..>4181)
                     /locus_tag="PK112_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10816.1"
                     /translation="ALTTVSLWLLAIHWVDLHWIVQPNFPGAGAVFSWIDAATMAGIG
                     GIFIWYVCRRHLRRPLVPVGDPGLQRSMQLTN"
BASE COUNT          798 a         1399 c         1316 g          668 t
ORIGIN      
        1 tccgttcttt cgccatgctc atgagacgac ctccggccgc accgcccggc cgcccggttc
       61 cacgttcacc gggatcaccc ggtcgtagtc atacggcccg tgggcgagcg acggcgtgaa
      121 ggggaaattc tcggtcggcg gcggcgatgg cgtctcccac tcgagcgtca gggcgcgcca
      181 cggattgctt cccgcccgcc ggccccgcgc gagcgagtgg atgaggtaga ttgccataat
      241 cacgaaaccc gccccgagaa tccacgaccc gaaagtcgag aacgcgtgga gcggctggta
      301 ctggtcgagg tagttgtagt agcgccgcgg cattccgtgg agaccgagga gaaactggct
      361 gccgaaggtg acgttgaaac cgacaaagat cagcgcgcac gcgactttcg cccatggctc
      421 gttgtacatc ctcccgaaca tcttcggcca ccagtggtgc agcccgccga ggaacgcgat
      481 caccgtcccg cccatcatca cgtagtggaa gtgggcgacg atgaagtagg tgtcgtggac
      541 gtggacatcg accgccagcg ccccgagcat gatcccggtg aacccgccga tgctgaacag
      601 gaacaaaaac gacagcgcgt agagcatcgg cgtggtcagc ttgatcgacc cgcggtagag
      661 cgtcgtcacc cagttgaaga ctttgatccc ggaggggatg ccgacgaaga aggtgaggaa
      721 cgagaagagc atggccgcca gctccgactg cccgctcacg aacatgtggt ggccccagac
      781 gagaaaactc acgaacgcga tcccgaggct cgaataggcc actgccttgt agccgaagat
      841 cttcttgtgc gcgtgggccg tgatcagttc gctcacaatc cccatgccgg ggagaatcat
      901 gatgtacacc gccgggtgcg agtagaacca gaagaagtgc tggtagagca ccgggtcgcc
      961 ccccatggcc ggatcgaaaa tcccgatccc cgccacccgc tcgatcacca gcagcagaag
     1021 cgtgatcccc agcaccggcg tcgccagcac ctggatgatc gcggtcgcat acatcgacca
     1081 cacgaacagc ggcatccggt accacgtcat ccccggcaga cggagcttgt gaatggtgac
     1141 gatgaagttc agccccgtga agatcgagga gaagccgagg atgaaggcgc ccagggtcat
     1201 cgagatcacc gaggtgtcgg tctgcgtgct gtagggcgtg tagaacgtcc acccggtgtc
     1261 ggccgcgccc gcgacgatcg agtagagcgc gaagagcgac ccgagcatga agatgtacca
     1321 gctcgcgagg ttcaaccggg gaaacgccac gtcccgcgcg ccgagcatga gcggcagcag
     1381 gaaattgccg agaatcgccg gcaccccggg gatgatgaac aggaaaatca tgatcgcgcc
     1441 gtgcagggtg aacagcttgt tgtaggtgtc ggcgcccacc aggtcccgcc cggggtggag
     1501 caactcgagg cgtatgagca tggccagcac gccgccgacg aagaagaaca ccgcgatcgc
     1561 ggccatgtag aggatcgcga tgcgcttgtg gtcgagcgtc aacagccacg atttccaccc
     1621 ccgggtggca ttcagataat taaccgccgt cgcttccgcc atgtcgttac tcaccggccg
     1681 cctcgctgtt ggtggtgtcc agagacttta tgaaagcaat gagggcgtcg atgtcccggt
     1741 ccgtcaggat tccctggtag gtgggcatga ccggctggaa cccggccgtg actctcgcct
     1801 gcggctcgag taccgactcc cggatatagt tctcgtcgac tgtgacaaag tcgcccgtct
     1861 gcagcgcctc ccggcggccc cacagcccct gccaggtcgg acccacgctc ggcttgccgt
     1921 ccagcgtgtg gcaggtgttg caggcgcggc tcgtgtagag ccggcggccg tagtcggccg
     1981 gtgactctcc ggccccgggt cccccggtcg aggcgaggaa ttccgcgaac tcccgctccg
     2041 acaccacctt caccttcgcc agcatctccg aatgccccgt gccgcagtac tccgtgcaga
     2101 acaggttgaa cgtcccgggc cgcgtcgcct caaaccacgt gaccgagtag cggttcggca
     2161 gcacgtccat tttcatgcgg aaatcgggca cgaaaaggct gtggatgacg tcgcgcgaag
     2221 acatgagcag cttgaccggc cggtcgaccg gggccaccag ttcgttgacg ctgttggcgc
     2281 cgttcgggta gtcgaacgtc cagaaccact tctgcgccgt caccttgacc tccagggcgt
     2341 cgcgcggcac gatcgtcatg cgcatgtagg cgcggaaacc ccatacgaac acgatgagga
     2401 tgagcacggt cggcaccgcc gtccacgtga tctccagcgc gagattgtgg tcccgtccgt
     2461 aggttgtccc cgtcggccgc cgccgccggt agcggatgat gaaaaacacc gtcaggccga
     2521 tgacgatggc gaagagcacg gccgacgccc agaagatgaa gtagaaaagg gaatcgactt
     2581 caccggccaa agtcgagccg gccggcggga aaaacaatgt tccggttgta tccatcgctc
     2641 cttctcatcc cgccggcgcc ggccgatgcc gcgtgcggta cgactcgcgc agccacaaaa
     2701 ccaagaggaa tccgcccagc ccgagcaccg tcagcaggcc gccgaccagc atgacattct
     2761 gcgccaggac gacatagccg ccggcgcccg gatcgtagtg gtagcagtac aaaatgacgc
     2821 ggtcgagcgt gctgccgatc ttcccgtcgg cggcctccac aagcgccagc ttcaggtcgc
     2881 gcgggttgta ctggatgccg tggaggtagc gcgacacgac tccctcgggg gagagcacgt
     2941 gcaccaccgc cgggtgggca tactcgtcgc ggctctcgtc gtagtagtag cggaacccga
     3001 ccgcgtcggc aagttgcgca atcgcggccg aatcgccggt gagaaacgcc cagctcgccg
     3061 gcggcgccgc cggcccgagc tgcgcgaggt agctggcctt cttggccgcc gccaggtccg
     3121 gggtctcgcc cgggtcgatg ctcaccgtga ccacccggta ctgccgcccc aggtccagcc
     3181 cgacccgcgg cagcgcctcg gtcaggccgt tgaacacgag gttgcacagc atcgggcact
     3241 cgtagtaccc gagcacgagc aggaccggcc ggtccccgcc gaaataatcg cccagcgcca
     3301 cctcccgccc cgcctcgtcg accagcccga gcccgcgggg gatggtgtcg ccgaggcgct
     3361 cgtggacatc gatgccgcgc agttcgggcg ggtcggtgcg gaccgtctgg ccggccgccc
     3421 ccccggccgc cgcgaggagc gcgaggaatc ctatatttcg gaggacacgt tgcatggcct
     3481 gtcttcgtaa ccgccggcgg gccggaaagg atccggaaaa ctgtctcctt aaatgatcca
     3541 tataatccat tttaccggcc gcccggagcc gccccccggg gccgtccggc attaccgcgc
     3601 tttcgcggta aacgcctcct gcgcctgaag ttccatggcc cgttcgatgg gaatccggta
     3661 gctccccgca gccccgtcct gcaccccgta cgattccagt tgctcgacct cccgcgcccg
     3721 cagctcccgc agcgccttcg actccggcgc ccgctgctgc tcgtaggaga tttcctcggt
     3781 cacggcgatg aagtactcgt tgagcacgac cgcgaacgcg atgacgagca gggtgagaag
     3841 caccccgacc acgagcgctt tggtcacgtt gacgtcgctc gtgtcgtacc cggccttttc
     3901 ctcccggccg ggcgccggtt tattttcggt gtccgtcata ttccgttcct aattcgtcag
     3961 ctgcatggac cgctggagcc cggggtcgcc caccggcacg agcggccgcc tcaggtgccg
     4021 ccgacagacg taccagatga agatgccgcc gatcccggcc atggtcgccg cgtcgatcca
     4081 cgaaaacacg gctcccgcgc cggggaaatt gggctgcacg atccaatgca gatccaccca
     4141 gtggatcgcc agcagccaca gactcaccgt cgtcagcgcc g
//
LOCUS       DAOOKS010000068         4167 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_25719_length_4167_cov_3.494163, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000068 DAOOKS010000000
VERSION     DAOOKS010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25719_length_4167_cov_3.494163"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..950)
                     /locus_tag="PK112_01815"
     CDS             complement(<1..950)
                     /locus_tag="PK112_01815"
                     /inference="COORDINATES: protein motif:HMM:NF006426.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HPC10817.1"
                     /translation="MNILDDIEKIRAHDPENMYNRIFDFPEQLADAVKLGKLWKFDAS
                     AFPGIKNIVTVGMGGSGIAGDLMRSYLRTKLLVPVDICRSYALPEYVDDETLVVAASY
                     SGNTEETLAAVDDALNRKAMLAAIATGGLLEEVAQLNDIPLAKLPGGLQPRAALGYLF
                     VMTMMFLEQVGHAKGFSKELEKVIPQLQKHREKYIEDNSTTINPAKNLAQKLHKKIVI
                     VYSGPTLTDAVGRRWKAQLCENAKTLAFANQFPEFNHNELVGWSPSIEELASHLAVVI
                     LRDLGDHPQVRQRMNIVGELIRSRGVEAIDVHSRGETELER"
     gene            complement(953..2728)
                     /gene="ptsP"
                     /locus_tag="PK112_01820"
     CDS             complement(953..2728)
                     /gene="ptsP"
                     /locus_tag="PK112_01820"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HPC10818.1"
                     /translation="MIGMDGKEYTIDGVPISSGIVLGTARVMLPGEAQVSEMPIPASR
                     VPREIDALQKAVDETLDELREVHQAAIRKIGGPVAKIFEAQVMIASDTEFLKRVRAAI
                     EEQRLGAAYVYQKLVQETVDPLQNSPDRYMRQVALDIEAVAARVVARLSGRIPQQKRF
                     APNTIMVGKSFSPNDVVSYRERKATGFVISEGGADSHMALIARALLLPVVLAEEAWQK
                     IPNNTRIIADAVSGRVIVNPSQATWEDYQRRRRRLGPTVLSRIKKLGRIPPVTADGVE
                     VAIGANLTLPGPADEILAHQRVQVGLYRTEFLYLANGAFPSEEAQYDYYRRIARTFGP
                     TPVVLRTFDLGGDKVFNGGGFPVEDNPALGLRGIRTMLAMREEFRTQIRAILRASAHG
                     NLRILLPMVTELEEIERARKIISQEKQKLRREAIPFGEDVPLGIMIEVPSAALTADTL
                     SRRVDFVSIGTNDLTQYTLAADRMNSHVAHLYNALHPAVLNLVWHTVKTCRANGVPVT
                     VCGELAGQRLALPLLIGMGVDVLSMAPARLVEVCRLIRKINSTRARDLVGPVLAGGTL
                     QAVVDRLRDYRETLEHVDPRMREDS"
     gene            complement(2790..3062)
                     /locus_tag="PK112_01825"
     CDS             complement(2790..3062)
                     /locus_tag="PK112_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HPC10819.1"
                     /translation="MITATATIVNKLGLHARPSAMLVTTASRFESEVWVTKDGLRVNG
                     KSIMGVMMLAAERGSQLLIEVNGPDEQEALQAVLRVIEGGFGERPD"
     gene            complement(3059..>4167)
                     /gene="dprA"
                     /locus_tag="PK112_01830"
     CDS             complement(3059..>4167)
                     /gene="dprA"
                     /locus_tag="PK112_01830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HPC10820.1"
                     /translation="SERHRRLVDTIGLLQVPGIGGGRFDRLVRAFGSAGAASAASIAE
                     LAAVPGISRALATAIHEQYDEASAEATAGQIAQLGWAVIFPDDADYPPMLSALEPPPP
                     LLFRVGEPAHASDRMIAIVGTRHPTERGRMFAHGLARALAESGIAVVSGMAEGIDTAA
                     HTGALDGGGRTVAVWGSSLDQVYPPTNRGLADRIRERGAIYSEYLPGTFPDRPHFPER
                     NRIISGMAWGVVVVEAGDRSGALITAEHALRQNRTVFAVPGPPGATASLGCNELLKQG
                     AGLVTSVEDIFRELPTLRGEVKVQRAAERPDLTPTEREIVALFTAGPVQLDDLARRLN
                     KPVAELTQFLLALELKGVVLELAGKRFTLSDEFA"
BASE COUNT          673 a         1380 c         1370 g          744 t
ORIGIN      
        1 cgctcgagct cggtctcccc gcgcgagtga acgtcgatcg cctccacccc gcgcgagcgg
       61 atcagttcgc cgacgatgtt catgcgctgg cgcacctgcg ggtggtcgcc aaggtcgcgc
      121 aggatcacga cggccaggtg cgaggcgagt tcctcgatcg acggcgacca gccgaccagc
      181 tcgttgtgat tgaattccgg gaactggttg gcgaaggcga gggtcttggc gttctcgcac
      241 aactgagctt tccagcgccg cccgaccgca tcggtcaggg tcggtccgct atagacgatc
      301 acgatcttct tgtgcagttt ctgggcgagg ttcttggccg ggttgatggt cgtcgaattg
      361 tcttcgatat acttctcccg gtgcttctgc agctgcggga tgaccttctc gagctccttg
      421 gagaacccct tggcgtggcc gacctgttcg agaaacatca tggtcatcac gaagaggtag
      481 ccgagggcgg cgcggggctg gagaccgccg ggcagtttgg ccagcgggat gtcgttgagc
      541 tgggccactt cctcaagcag accgccggtg gcgatcgcgg cgagcatggc cttgcggttg
      601 agcgcgtcgt ccaccgccgc gagcgtctcc tcggtgttgc cgctgtagga ggcggcgacc
      661 accagggtct cgtcgtcgac atactcgggc agcgcgtagg agcggcagat gtcgaccggc
      721 acgagcagtt tggtgcggag gtagctgcgc atgaggtcgc cggcgattcc ggacccgccc
      781 atgcccacgg taacgatatt cttgatgccc gggaacgcgc tcgcatcgaa cttccagagt
      841 ttgccgagtt tcaccgcatc cgccaactgc tcgggaaaat cgaagattcg gttgtacatg
      901 ttttccggat cgtgggcgcg gatcttctca atgtcgtcga gtatattcaa aatcagctgt
      961 cctctctcat tctggggtcc acgtgttcca gggtctctct atagtcacgc aggcggtcca
     1021 ccaccgcctg cagcgtgccg ccggcgagca ccggcccgac caggtcccgc gcccgggtgg
     1081 agttgatttt gcggattaaa cggcagacct cgaccaggcg cgccggcgcc atcgacagca
     1141 cgtccacgcc cataccgatc agcaggggca gggccagccg ctggccggcc agctcgccgc
     1201 acacggtcac cggcacgccg ttggcccggc aggtcttgac cgtatgccag accaggttca
     1261 gcacggccgg gtgcagggcg ttgtacaggt gcgccacgtg gctgttcatc cggtccgcgg
     1321 ccagcgtata ctgcgtcaga tcgttcgtcc cgatcgacac gaagtccaca cgccgactca
     1381 acgtgtcggc cgtgagggcg gccgatggga cttcgatcat gatccccagg ggaacgtctt
     1441 cgccaaaggg gatagcttcc cgccgaagtt tctgcttctc ctgcgaaatg atcttccggg
     1501 cccgctcaat ttcctccagc tcggtgacca tcggcagcag gatccgcaga ttgccgtggg
     1561 cggacgcgcg gaggatcgcc cggatctggg tgcggaattc ttcccgcatc gccagcatag
     1621 tgcggattcc ccgcagtccc aaggcggggt tgtcctcgac cgggaagccg ccgccgttga
     1681 acaccttgtc gcccccgagg tcgaaagtcc gcagcaccac cggcgtcggg ccgaaagtcc
     1741 gggcgatccg ccggtagtaa tcgtactggg cctcctccga gggaaacgcc ccgttcgcga
     1801 ggtacaagaa ctcggtgcgg tagagaccga cctggacgcg ctggtgggcg aggatttcat
     1861 cggccgggcc gggcagcgtc aggttggccc cgatcgccac ctcaacgcca tcggccgtga
     1921 ccggcgggat gcggccgagc ttcttgatgc ggctgagcac ggtcggcccg agccgtcgcc
     1981 ggcgccgctg gtaatcttcc caagtcgcct ggctggggtt gacgatgacc cggccggaca
     2041 cggcatcggc gataatccgg gtgttgttgg ggattttctg ccatgcctcc tcggccagca
     2101 cgaccggcag caaaagagcg cgggcgatga gcgccatgtg agaatcggcg cccccttcgc
     2161 tgatgacgaa gccggtggct ttgcgctccc ggtacgagac cacgtcgttg ggggaaaacg
     2221 atttccccac catgatggtg ttgggggcga accgcttctg ctgggggatc cgccccgata
     2281 gccgggccac cacccgggcc gcaaccgcct cgatatcgag cgctacctgc cgcatgtaac
     2341 ggtcggggga gttttgcagc gggtcgaccg tctcctgcac cagcttctgg tagacatagg
     2401 cggcgccgag ccgctgctcc tcgatggcgg cccgcacccg cttgaggaac tcggtgtcgc
     2461 tggcgatcat gacctgagct tcgaatatct tcgccaccgg accgccaatc ttgcggatgg
     2521 ccgcctggtg gacctcgcga agctcgtcga gggtctcgtc gaccgccttc tgcagggcgt
     2581 cgatctcccg gggcacccgg gacgccggga tcggcatttc cgatacctgc gcctcgcccg
     2641 gcagcatcac ccgggcggtt ccgagaacaa tcccgctgga gatcggcacc ccgtctatgg
     2701 tgtattcctt gccatccatg ccgatcatac ccctttccgg ggtaaccata cattatagga
     2761 agccccgcag actaaaggca agcggaatat cagtcgggcc gttccccgaa ccccccctcg
     2821 atcacgcgga ggactgcctg gagggcctcc tgctcgtcgg gtccgttcac ctcgatgagc
     2881 agttgtgagc cgcgctcggc cgccagcatc attaccccca tgatcgattt cccgttcacc
     2941 cgcaaaccgt ctttggtgac ccagacctcg gactcgaagc gcgatgcggt ggtgaccagc
     3001 atggccgagg gacgggcgtg gagacccagt ttgttgacga tcgtcgcggt ggccgtgatc
     3061 atgcgaattc gtcgctcagc gtgaagcgtt tgccggccag ttcgaggacg acccccttga
     3121 gctccagcgc cagcaggaac tgggtgagtt cggcgaccgg tttgttcagg cggcgcgcga
     3181 ggtcatcgag ctgcaccggt ccggccgtga agagggccac gatttcccgc tcggtcggcg
     3241 tcaggtccgg gcgctcggcc gcccgctgca ccttgacctc gcctctcagg gtgggcaact
     3301 cgcgaaaaat gtcctcgacc gaggtcacca gcccggcccc ctgcttgaga agttcattgc
     3361 agccgaggct ggcggtcgca cccggggggc cggggacggc gaacaccgtg cggttctgcc
     3421 gcagggcgtg ttccgccgta atcagcgccc ccgaccggtc tcccgcctcg accaccacca
     3481 ctccccaggc catgccggag atgatgcggt tgcgctcggg gaaatgcggg cggtcgggga
     3541 acgtgccggg gaggtactcc gagtagatcg ccccccgttc cctgatccgg tcggccaggc
     3601 cccggttggt cggggggtaa acttgatcga gcgagctgcc ccagacggcg accgtgcggc
     3661 cgcccccgtc gagcgctccc gtgtgggcgg cggtgtcgat cccctcggcc atgcccgaca
     3721 cgaccgcgat gcccgactcg gccagcgccc gcgccaggcc gtgcgcgaac atccgcccgc
     3781 gttcggtcgg gtggcgcgtg cccacgatcg cgatcatgcg atccgaggcg tgcgccggct
     3841 ctccgacccg gaacaaaaga gggggtggcg gctcgagtgc gctcaacatc ggcgggtagt
     3901 ccgcatcgtc gggaaaaatc acggcccagc ccagctgcgc aatctggccg gcggtcgctt
     3961 ccgccgacgc ctcgtcatac tgctcatgga tagcagtcgc cagggcgcgg gaaatgcccg
     4021 ggacagccgc cagctcggcg atcgaggccg cggacgccgc gccggccgaa ccgaaggcgc
     4081 ggacaagtcg gtcgaatcgg ccgccgccga tgccgggcac ctgcagcagc ccgatcgtgt
     4141 cgactaatcg tctatgtcgc tcgctgt
//
LOCUS       DAOOKS010000069         4143 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_25912_length_4143_cov_3.863992, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000069 DAOOKS010000000
VERSION     DAOOKS010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4143
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25912_length_4143_cov_3.863992"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..904
                     /locus_tag="PK112_01835"
     CDS             <1..904
                     /locus_tag="PK112_01835"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HPC10821.1"
                     /translation="RLLAAPGLGARSVARLLLRLAAEQLPLEDFVALPADHLVREYAL
                     APASADALASAGEEARELAEHLPAHRIRMVTIVDDGYPELLARRLGRTAPPVLFTRGD
                     LGLLRVPMVACAGARECSAPGLAFARALAAALVRHGANIVSGYAPGIDTAAHGGALAA
                     GGVTTAVLASGILHFTPRPGLASLGDETNTLVVSEFFPTMPWRSHSAMQRNRTICAMA
                     HAMVIVESGVSGGTFAAGRAALEVGCPLYVVDWQDPPPSAEGNRLLIRRGGVPVADLS
                     GALRELTRRLHSEGPGPVTPELFG"
     gene            991..2544
                     /locus_tag="PK112_01840"
     CDS             991..2544
                     /locus_tag="PK112_01840"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HPC10822.1"
                     /translation="MPRRLRRLPMKEDLMSAQAVYDELTRKLREMALLSSAGSVLHWD
                     QQTYMPPEAAQFRADQLGTISGLVHRMFTDPKIGDMIGTVENSDLAKGDTPEAANIRE
                     LRHDYDKMTKLPNSLVEELTRTTAMAQQVWAEARRKSDFAMFLPHLEKVLALTRRKAD
                     AYGWKDEPYNALIDDYEPGANVREVGEVFESLRKELVVLNEKIRNAPRKPDHTIVERE
                     YEVERQKVFGESVATAMGFNFRAGRLDITTHPFCTGIGYGDTRILTRYNPNRLNDALF
                     GIMHEAGHGLYEAGLLKSEYYGTPMAESVSLGIHESQSRMWENQVGRSKSFWKYFFPQ
                     AQRLFRKSLGDVTLDRFYGAINYVTPSYIRVEADEATYNLHILLRFEIERALLTGDMK
                     PADAASEWNARFEKYFGLKVDKDAHGVLQDVHWSAGLVGYFPTYTLGNLYSAMFFAQA
                     RKEMPDLDLQFERGELLPLREWLREKIHRHGRRYRANELCRKITGKSLDYKPLVVYMT
                     EKYSEIYGF"
     gene            complement(2618..2833)
                     /locus_tag="PK112_01845"
     CDS             complement(2618..2833)
                     /locus_tag="PK112_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10823.1"
                     /translation="MSDDGRAVESAGPAKPAVGKARWNLLRFPRSWKEVRALGWRMIV
                     AFIFFYLIRDTLLYIVFPYLAYKGIFD"
     gene            2981..>4143
                     /gene="trkA"
                     /locus_tag="PK112_01850"
     CDS             2981..>4143
                     /gene="trkA"
                     /locus_tag="PK112_01850"
                     /inference="COORDINATES: protein motif:HMM:NF007039.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HPC10824.1"
                     /translation="MRIVIIGGGVVGFSLAEHLLRDGHTLSLVELDPHLCRMIEEKLD
                     LQVVSGSGASPVTLREAGIEGADMMLAVTPSNEVNIVACSIAALHEVPSRIARLRGQE
                     FSARGGLVDLTRMGITALIQPEQAVADHILQFMETPHAVESANFEGGRILLRGYAVKD
                     TMPLAGKTLREIRAHIDPAVVLFAAIVRAGKGMIPDGNTRIEPGDIVYALFPRESLGV
                     FLDLVAIERKESRKVIVTGDAFFTLELAEALERTPHKVTLVDPSREHAEKAAAMFNRT
                     EVIHGDCTETDLLRELNVAAASFFIAASDEADYNVFSSLLAKAEGAREVIAVSTESRH
                     DRLFNSIGIDHVVNPRLITAREILEIISRGHIGAVVKLSDVDIEAVRFTVEEP"
BASE COUNT          729 a         1495 c         1226 g          693 t
ORIGIN      
        1 gcgcttgctc gccgcccccg gcctcggggc gcgctcggtc gctcgcctcc tgctccggct
       61 cgcggcggaa cagctcccgc ttgaggattt cgtcgccctc cccgccgacc acctcgtgcg
      121 ggagtatgcg cttgcgccgg cgtcggccga cgccctcgcg agcgccgggg aggaggcgcg
      181 ggaactggcc gagcatctcc cggcccaccg gatccggatg gtgacgatcg tggacgacgg
      241 ctacccggag cttttggccc gccgcctcgg ccgcaccgcg ccgcccgtgc ttttcacccg
      301 gggcgacctc ggcctgctgc gggtgccgat ggtcgcctgt gcgggcgccc gcgagtgctc
      361 cgcgcccggc ctggcctttg cccgcgcgct tgccgccgcg ctcgtccgcc acggcgccaa
      421 tatcgtcagc ggctacgccc cgggcatcga caccgccgct cacggcggcg ccctcgccgc
      481 cggtggcgtc accaccgccg tcctggcctc gggaattctc catttcaccc cgcggcccgg
      541 cctggcgtcc ctcggcgacg agaccaacac cctggtcgtg tcggagtttt tcccgaccat
      601 gccgtggcgg agccacagcg caatgcagcg caacaggaca atttgcgcga tggcccatgc
      661 tatggtgatt gtggaatcgg gagtttcagg ggggactttc gccgccggcc gggccgcgct
      721 cgaagtcggc tgccccctct acgtcgtcga ctggcaagat ccgccgccga gcgccgaagg
      781 caaccgcctc ctcatccggc gcggaggagt gccggtcgcc gacttatcgg gcgccctgag
      841 agagctgacc cgccgccttc actcggaagg tcccggcccg gtcactcccg agttgttcgg
      901 ctagccgtcc gtgggcgcgc ttttctcgcc gccccggttg ccttgccggc cgattctccg
      961 tatcttgggc cttcaaattg cgatggaacc atgccgcgcc ggctgcgccg gctgcccatg
     1021 aaggaggatc ttatgtccgc ccaggccgtt tacgacgaac tcaccagaaa actcagggaa
     1081 atggcgctct tgagttctgc cggaagtgtc ctgcactggg accagcagac ctacatgccg
     1141 cccgaggccg cgcagttccg cgccgaccag ctcggtacga tctccgggct cgtccaccgc
     1201 atgttcaccg acccgaaaat cggcgacatg atcgggacgg tcgagaactc cgatctcgcc
     1261 aagggggata cgcccgaagc cgccaacatt cgcgaactgc gccatgacta cgacaaaatg
     1321 accaagctcc ccaacagcct ggtcgaggaa ctcacccgca ccaccgccat ggcccagcag
     1381 gtctgggccg aggcccggcg caaatccgat ttcgccatgt tcctccccca cctcgaaaag
     1441 gtgctcgcgc tcacccgccg gaaagcggac gcctacggct ggaaggacga gccctacaac
     1501 gccctcattg atgattacga gccgggggcg aacgtccgtg aggtcggcga ggtgttcgaa
     1561 agcctccgca aagaactcgt ggtcctgaac gagaagatcc gcaatgcccc gcggaagccg
     1621 gaccacacca tcgtggaacg cgagtacgag gtcgagcggc agaaagtgtt cggggagtcg
     1681 gtggccacgg ccatgggctt caacttccgc gccgggcgcc tcgatatcac cacgcatccc
     1741 ttctgcaccg gtatcggcta cggcgacacc cgcatcctca cccgctacaa tcccaaccgg
     1801 ctcaacgatg cccttttcgg gatcatgcac gaggccggcc acggcctgta cgaggcggga
     1861 ctcctcaaga gcgagtacta cggcacgccg atggccgagt cggtgtcgct cggcatccac
     1921 gaatcccagt cgcgcatgtg ggaaaaccag gtcggccgct ccaaatcttt ctggaagtac
     1981 ttcttcccgc aggctcagcg gctgttccgg aaatccctcg gcgacgtcac cctcgaccgg
     2041 ttttacgggg cgatcaacta tgtgaccccg tcgtacatcc gggtcgaggc cgacgaggcg
     2101 acctacaacc ttcacatcct gctgcgcttc gaaatcgaac gggccctgct caccggcgac
     2161 atgaaaccgg ccgatgccgc ctctgagtgg aacgcccgct tcgagaagta cttcggtctg
     2221 aaggtcgata aagacgctca cggcgtcctc caggatgtcc actggtcggc cggcctggtc
     2281 ggctacttcc cgacctacac gctcgggaac ctctactcgg ccatgttctt cgcccaggcc
     2341 cgcaaggaga tgcccgacct cgacctccag ttcgaacgcg gcgaattgct cccgctccgg
     2401 gagtggctgc gggagaaaat ccaccgccac ggccgccgct accgggccaa cgagctgtgc
     2461 cggaagatca cggggaaatc ccttgactac aagccgctcg tggtgtatat gacagaaaag
     2521 tattcggaaa tttacggttt ctaacccagc caaagagacc gaacgaaaag ccggcaacct
     2581 ccgtgtcgcc ggctttcttt tgcccgctcg cctcgattca gtcgaatatc cctttgtacg
     2641 ccaggtaggg aaaaacgatg tacaggagcg tgtcccggat caggtagaag aagatgaagg
     2701 cgacgatcat ccgccagccc agcgcacgga cctccttcca cgaccgggga aaccgcagca
     2761 ggttccagcg cgctttgccg accgcgggct tcgccgggcc cgccgattcc accgctcgtc
     2821 cgtcgtcact catacgagcg tataacagac cgctcccggc cgggttccgc ggcctcggcc
     2881 gcctgcgcat aaactatttg attccccgcc ccttcgcatg catgttgcgg gtggcccggc
     2941 ggggtcggcg gaccccccga acacaaacaa tggaatcgtc atgcgaattg taatcatcgg
     3001 cggcggggtc gtcggattca gcctggccga acacctgctc cgcgacggcc acactctgtc
     3061 gttggtcgaa ctcgacccgc acctgtgccg gatgatcgag gagaaactcg acctccaggt
     3121 ggtcagcgga tcgggcgcct cccccgtgac gctccgggag gcggggattg agggtgccga
     3181 catgatgctc gccgtgaccc cgagcaacga ggtcaacatc gtcgcctgct ccatcgccgc
     3241 tctgcacgag gttcccagcc ggatcgcccg cctccgcggc caggaattct cggcccgcgg
     3301 cggcctggtc gatctcacac ggatggggat caccgccctc atccagcccg agcaggcggt
     3361 ggccgaccat atcctgcagt tcatggagac cccccacgcc gtcgaatcgg ccaatttcga
     3421 gggcggccgg atcctcctcc gcggctacgc cgttaaagac accatgcccc tggccggaaa
     3481 aaccctccgc gaaatccgcg cccacatcga ccccgcggtc gtcctcttcg ccgctatcgt
     3541 ccgggccggg aaggggatga tccccgatgg caacacccgc atcgaacccg gggacatcgt
     3601 ctacgctctc ttcccccgcg aatcgctcgg cgtcttcctc gacctcgtgg cgatcgagcg
     3661 caaggagagc cgcaaagtga tcgtcaccgg cgacgctttc ttcaccctcg agctggccga
     3721 ggcgctcgag cggacaccgc acaaggtgac cctcgtcgac ccgtcgcgcg agcatgctga
     3781 gaaggccgcc gcgatgttca accgcaccga ggtgatccat ggcgactgca ccgaaaccga
     3841 cctcctgcgc gaactgaacg tcgccgccgc ctccttcttc atcgccgcat ccgacgaagc
     3901 cgactataac gtcttctcgt cgctgctggc caaggccgag ggggcccgcg aggtcatcgc
     3961 cgtgtccacc gagtcgcgcc acgaccggct cttcaattcc atcgggatcg accacgtggt
     4021 caacccccgc ctcatcaccg cccgcgagat cctcgagatc atatcccgcg gccacatcgg
     4081 cgccgtcgtg aaactctccg acgtcgacat cgaggccgtc cgctttaccg ttgaagagcc
     4141 cag
//
LOCUS       DAOOKS010000070         4139 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_25953_length_4139_cov_3.478208, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000070 DAOOKS010000000
VERSION     DAOOKS010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4139
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25953_length_4139_cov_3.478208"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="PK112_01855"
     CDS             <1..582
                     /locus_tag="PK112_01855"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPC10825.1"
                     /translation="IDSANPDLRAMAAMVEQERAGVRAANAARYPGLTVGADYLFAGR
                     SADPGMTGDGADTWVLSASVSLPLWLGKNQARSRAAEARERAAAYDLTESRNRLRTYA
                     ADVMYQYRDARRRVELYAGGLLPKAEEGLNAAYTAYQAGALDFLSLLDAQRQLLEFQL
                     AHSEAVAAAAVRLAELEMLAGGRLTGETPATER"
     gene            623..2575
                     /locus_tag="PK112_01860"
     CDS             623..2575
                     /locus_tag="PK112_01860"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPC10826.1"
                     /translation="MNDFEAAGGRSPRSRIDRFRRTRLGLAVQIALVMGVFALGLMIG
                     GGGSPAPSADSGRPGAEEANAPVLWTCSMHPQIQLPKPGKCPICFMDLIPVETGRGSG
                     RLGPRQIAMSEAARRLARIETAPVRRADAEADIRLVGQIDYDETRLAYIAARVPGRID
                     TLFADYTGVTVRRGAPLVSLYSPDLLSTQQELLQAKKALAAAAQSAGLVRATAESTLA
                     AARERLRLWGLTAEQIERIEAGGQPSDHLTILSPISGTVIEKEALEGEYVETGMRIYT
                     VADLSRLWVILEAYESDLPWLREGQRFTFTAQALPGETFSAVVSFIDPVIDPESRTAR
                     VRAAVDNAAGRLKPKMFVSGEVAARLGSESGLARSTGRPDAPLLIPTSAPLVTGTRAV
                     VYVKTAEDGDGPVYEGRDIVLGPRAGDFYVVANGLAEGEEVVSRGAFKIDAELQIQAK
                     PSMMSPAPLARRAADSASVHGDHPAAGADVAISPDARASLSPLYEAYFEVQMALAKDQ
                     FEASRRGLDDLAAKTAAVDMSLFAGRHTHCGWTCRGACRPPRGRARPPATSPPCATLS
                     AIFPPPPSTCTGRSVTPTTAPSTSHTARWLSTTPAPTGCKPKGLSGTPTGGPPCCSAG
                     KSATRFPRSRPEPGSPPLTPDRAADV"
     gene            2572..4139
                     /locus_tag="PK112_01865"
                     /pseudo
     CDS             2572..4139
                     /locus_tag="PK112_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008099485.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
BASE COUNT          665 a         1436 c         1361 g          677 t
ORIGIN      
        1 attgactcgg ccaatcccga ccttcgggcg atggcggcca tggtggagca ggagcgggcc
       61 ggggtgcggg cggcgaatgc cgcacggtat cccggcctga ccgtcggcgc cgactacctc
      121 ttcgccggcc ggtcagccga cccggggatg accggcgacg gtgcggacac ctgggtgctc
      181 agtgcatccg tctccctccc cctttggctc ggcaagaacc aggcccggtc gcgcgcggcc
      241 gaggcccgcg aacgggccgc cgcttatgat ctgaccgagt cgcgcaaccg gctgcgtaca
      301 tatgccgccg acgtgatgta ccagtaccgc gacgcgcgcc gtcgggtcga gttgtacgcc
      361 ggcggccttc tgcccaaagc cgaggagggg ctcaatgccg cctacacagc ctaccaggcc
      421 ggggcgctcg attttctgag tctcctggac gcccagcggc aactgctcga atttcaactg
      481 gcccacagcg aggctgtcgc cgccgccgcc gtccgcctgg cggagcttga gatgctcgcc
      541 ggcggccgac tcaccggcga gacgccggcg accgaacggt gacatacgat gattccgaaa
      601 acttcgcaca gagagaacag ctatgaacga ttttgaggct gccggcggcc gctccccccg
      661 gagccgaatc gaccggttcc gccgaacccg gctcggcctc gcggtgcaga ttgcgctggt
      721 catgggcgtg ttcgcgctcg gcctgatgat cggcgggggc gggagtcccg ctccctccgc
      781 cgacagcggg cgccccggcg ccgaagaggc gaacgcccca gtgctgtgga cctgctccat
      841 gcacccgcag attcaactgc ccaaaccggg gaagtgcccg atctgcttca tggatcttat
      901 cccggtcgag accgggcgcg gcagcggccg cctcggtccc cgccagattg cgatgagcga
      961 ggcggcccgg aggctggccc gcatcgagac cgcgccggtc cggcgcgccg acgccgaggc
     1021 ggacattcgc ctggtcgggc agatcgacta tgacgaaacc cgcctggcgt acattgcggc
     1081 ccgcgtgccc ggccgtattg acacgctttt cgccgactac accggggtca ccgtgcggcg
     1141 gggggcgccc ctggtttcgc tctactcgcc cgatcttttg tcaacccagc aggagctctt
     1201 gcaggcgaag aaagcgctgg ccgcagcggc gcagagcgcc ggactggtgc gcgcgacagc
     1261 cgaatcgacc ctggccgcag cccgcgaacg gctccgtctc tggggactga ccgccgagca
     1321 gatcgagcgt atcgaggccg gcggccaacc cagcgaccac ttgacgattc tctcgcccat
     1381 cagcggcacg gtgatcgaga aagaagctct cgagggggaa tatgtcgaga ccggcatgcg
     1441 catctatacg gtcgccgatc tctcccggct gtgggtgatt ctggaagcat atgagtccga
     1501 tctcccctgg ctgcgcgagg ggcagcggtt caccttcact gcccaggcgc tacccggcga
     1561 gacgttctcc gcggtcgtat cgttcatcga cccggtcatc gacccggaga gccggaccgc
     1621 gcgcgtccgg gcggcggtgg acaacgctgc gggccggctg aaaccgaaga tgtttgtctc
     1681 gggcgaggtt gcggcgcggc ttggctccga gagcggcctc gcgcgctcca ccggccggcc
     1741 ggacgccccc ctgctcatcc ccacctcggc gccgctggtg accggcacgc gggcggtggt
     1801 ttacgtcaaa acggccgagg acggcgacgg cccggtgtac gaagggcgcg acatagtcct
     1861 cggtccgcgg gcaggggatt tctatgtcgt cgccaacggg ctggccgagg gagaggaagt
     1921 ggtctcgcgc ggggcgttca agatcgacgc cgagctgcag attcaggcga aaccttcgat
     1981 gatgtcccct gcgccgctcg cccgccgggc ggccgacagc gcgtctgtgc acggcgacca
     2041 tcccgccgcc ggcgcggacg tcgcgatctc gccggacgcc cgggcatccc tctccccgct
     2101 ctacgaggcg tatttcgaag tccagatggc tctcgcgaag gatcaattcg aggcttcgcg
     2161 gcgcggcctg gacgatctgg cggcgaagac ggcggcggtt gacatgagcc tcttcgccgg
     2221 ccggcacacg cactgtggat ggacctgtcg cggcgcctgc agaccgccgc gcgggcgggc
     2281 gcggccgccg gcgacatcgc cgccgtgcgc aacgctttcc gcgatctttc cgccaccgcc
     2341 atcgacctgc accgggcgtt cggtcacgcc gacgaccgcc ccttctacct cacatactgc
     2401 ccgatggctt tccacaacac cggcgcctac tggctgcaaa ccgaagggat tgtctggaac
     2461 tcctactggg gggccgccat gctgcagtgc ggggaaatcc gcgacacgct tcccgcgctc
     2521 tcggccggag ccgggatcac cgcccctgac tccggacagg gccgctgacg tatgagcaac
     2581 tcccgaccgc ccgaccacag cgcgcgcgcg tcgctgctgg accgcctgat cggctcctgc
     2641 ctggacaaca agctggtcgt gatcctcgtc gccctggcgg tgatcgcctg gggggtgatc
     2701 gtcgccccct tcgattggca catccccggt ctcgcccgcg ccccgatccc ggttgacgcc
     2761 atccccgata tcggcgagaa ccagcagatc gtgttcaccg agtggatggg tcggtcgccg
     2821 caggacgtcg aggaccagat cacctacccc ttgaccacga cgctgctcgg catccccggc
     2881 gtcaagaccg tccgcagcta ctccatgttc gggttttcga cgatttacgt catcttcaag
     2941 gaggatgtcg agttctactg gtcccgctcc cgcgtgctcg agaaactcaa ttcgcttccc
     3001 tcggggactc tgcccgcagg cgtgcaaccg atgctcggac ccgacgccac cgcgctcggc
     3061 caggtcttct ggtacacgct tgaggggcgc gacccggagg gacgacccgc cggcgggtgg
     3121 gacctcgaag agcttcggtc cattcaggac ttcacgtccg ctacgctctc cagtccgccg
     3181 acggggtctc ggaggtcgcg tcggtgggcg ggttcgtgcg cgaataccag atcgacgtca
     3241 gtcccgacgc catgcgcgcg gccggagtga cgctcgaaga cgtgttcgag gcggtgcaga
     3301 agagcaacat cgacgtcggc gccggcacga tcgagatcaa caaggtcgag tacgcgatcc
     3361 gggggcttgg cttcatcgaa aacctcgacg acctccgcgc aacggtagtc aagtcggtgc
     3421 ggggcgtccc ggtccgcatc gccgacgtgg ccacggtgca gctcgggccg gcgccgcggc
     3481 gcggggcgct ggacaaggga ggcgcggagg cggtcggcgg cgtggtagtc gtgcgcttcg
     3541 gtgaaaaccc cctgcaggcg atcaaaaacg tcaaagccaa aattgcggaa atcgaacccg
     3601 gtttgccgaa gaagacgctc cccgacggca ccacgagcca ggtgaagatc atccccttct
     3661 acgaccgcac cgggctcatc tacgagacgc tcgacaccct caacatggcg ctggtcgacg
     3721 aaatcctggt caccatcatc gtcatcctct tcttcgtcat gcacctgcgc agttccctcc
     3781 tcatctccgg catgctcccc ctggccgtgc tgatgacctt catcgggatg aaattgttcg
     3841 gtgtcgacgc caacatcgtg gccctctccg ggatcgccat cgccatcggc gagattgtcg
     3901 acatgggggt ggtgttgtgc gagaacatcc tgcgccacct ggacgaggcg ccgcccgagg
     3961 ccaaccgccg cgacgtcatc taccgcgctt cgaccgaagt cgggggcgcc gtcctcacgg
     4021 cgatcgccac gaccgtggtc tcgttcctgc cggtgttcac gctggaggcg gcggagggga
     4081 agttgtttca gccgctggcg tacacgaaga cctttgcgct ggtagcgtcg atcatgatc
//
LOCUS       DAOOKS010000071         4116 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_26149_length_4116_cov_2.497168, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000071 DAOOKS010000000
VERSION     DAOOKS010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4116
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26149_length_4116_cov_2.497168"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="PK112_01870"
     CDS             complement(<1..226)
                     /locus_tag="PK112_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HPC10827.1"
                     /translation="MNEMFTEGARKAIEYARDEASRLRHDYIGTEHLLLGLIRLGEGR
                     AVEIIVNLGLDLNDLKQSVEEVVQPSGGTMT"
     gene            complement(302..1339)
                     /locus_tag="PK112_01875"
     CDS             complement(302..1339)
                     /locus_tag="PK112_01875"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: protein motif:HMM:NF002194.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0046314 - phosphocreatine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HPC10828.1"
                     /translation="MVKSPAVWLSGKGEESAAVLSSRVRLARNIAGCKYPPSADSETG
                     TRIVNYVDSTVSRSDLLGRGRYVRAGDISPLDRDFLVERHLISPAFLSGDLPKALYIG
                     SQEQVSIMVNEEDHLRIQALASGLDPRGAYAAAKQYDQEIGRHLEYDYDPDFGFLTAC
                     PTNAGTGMRASVLIHLPGLVLTREIDHVIRHITATGLIVRGFYGEGSDVLGNLFQISN
                     QNTLGITEDDILAQIMRVTRDIIEKEAAARRRLVDEASDMIEDKIWRAYGILKHARVL
                     TSEEVMNLLSAVRLGQAMGIIDFLSVAQINDMLLLSQPAHLQRFYGSEMDNNRRDFVR
                     AQMVREKLRNT"
     gene            complement(1380..1910)
                     /locus_tag="PK112_01880"
     CDS             complement(1380..1910)
                     /locus_tag="PK112_01880"
                     /inference="COORDINATES: protein motif:HMM:NF014232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HPC10829.1"
                     /translation="MLCQDCNKNEATVHLTQIVNNQKTMLNLCKQCAAARGFHSPLEN
                     VPFPLAEILSGFASSPQGAPPVDTGDPLTCPGCGLTFQAFTRQGRFGCGECYKAFRSR
                     LEPIMRKIHGASLHRGQRPVTVIDGAADDDAIPVKEEERLEAELAKAIESEDFERAAE
                     IRDKLKTIREESSLHR"
     gene            complement(1929..2621)
                     /locus_tag="PK112_01885"
     CDS             complement(1929..2621)
                     /locus_tag="PK112_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPC10830.1"
                     /translation="MDTHDDTILRAVDLHREFRTPDATLHVLRGINLTMPRGDTVAVT
                     GASGVGKSTLLHLLGGLDRPTAGAVEIDGTRVDNQSEADLAAFRNRNVGFVFQFHYLL
                     EDFSAAENVMMPVLVAGQSRTQARRRAELLLDMVGLSERKNHRPAQLSGGEQQRVAVA
                     RALANDPRIVLADEPSGDLDSATGRMLHELLFRLNREQSIALLIATHNRELADRCNRT
                     LEIVEGKFCERS"
     gene            complement(2721..3947)
                     /locus_tag="PK112_01890"
     CDS             complement(2721..3947)
                     /locus_tag="PK112_01890"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPC10831.1"
                     /translation="MGYERFVARRYLRSGRFFISVSTWITILGVALGVGVVCFVMSMH
                     NGFEAEIRTRLLGTTSHISIFPVREEFIANYRALVDSLARVPGVAAASPFIYYKAAIA
                     SESAGDGIIVRGIVPELERRASQIEKNIVGGTYNFNEIVIADDTVPGIILGKNLADRL
                     GAVLGSEVVLYSLKGDEIRSNTRPRVAKFYVAGIFESGMFEFDSELAYISLPAAQRLF
                     RTGDAVTTIHLRLDDVYRAEAIRPVVDSLLEHRFEVVPWNVLHRNLFAWIAIEKKVLF
                     LGFILIVLVAAFSIISTLVMLTMEKRAEIGILKTIGSTPGSIRKIFVYKGLGIGAGGV
                     AGGWGLALAAAWLQNRYHLVSLPPDIYFISYLPIDIHPVDFLLAGLVTVAICFLAALY
                     PAHQASRVSVVEVLRR"
BASE COUNT          739 a         1201 c         1418 g          758 t
ORIGIN      
        1 tggtcatggt gccgccggag ggctggacca cctcttccac ggactgcttc aggtcgttga
       61 ggtcgagtcc gaggttgacg ataatctcga ccgctcggcc ctcacccagc cggatcagtc
      121 ccagcagcag gtgttcggtt ccgatgtagt cgtgccggag ccgggaggcc tcgtcgcggg
      181 cgtactcgat ggctttgcgg gcaccttcgg tgaacatctc attcatcgcg aaccttcctt
      241 gcgtctatgt ccaagacttc ttgtcacgcc aatatgcacc tgtaaataca tcatatcggg
      301 cctatgtgtt ccggagtttc tcgcgcacca tctgggcgcg gacgaaatcg cgccggttgt
      361 tgtccatctc ggagccgtag aaccgctgga ggtgggcggg ctgcgacagc aggagcatgt
      421 cgttgatctg ggccacgctg aggaagtcga tgatgcccat ggcctggccc agccgcacgg
      481 cagagagcag gttcatcacc tcttcggacg tgagcacgcg ggcgtgtttg aggatgccgt
      541 aagcccgcca gatcttgtcc tcgatcatat cggaggcctc gtcgaccagc cgccggcggg
      601 cggcggcctc cttctcgatg atgtcgcgcg tcacgcgcat gatctgcgcc aggatgtcgt
      661 cctccgtgat gccgagcgtg ttctggttgg agatctggaa gaggttcccc agcacgtcgg
      721 aaccctcccc gtaaaacccg cgcacgatga ggccggtcgc ggtgatgtgg cggatgacat
      781 ggtcgatctc gcgggtgagg acgagcccgg ggaggtggat gaggaccgag gcgcgcatgc
      841 ccgtgccggc gttggtcggg caggccgtga gaaaaccaaa gtccgggtcg tagtcgtact
      901 cgaggtggcg gccgatctcc tggtcgtact gcttggccgc cgcgtacgca ccccgcgggt
      961 cgagtccgga ggccagcgcc tgtatccgca ggtggtcttc ctcgttgacc atgatcgaca
     1021 cctgttcctg cgacccgatg tagagggctt tggggaggtc gccggagagg aaggcggggg
     1081 agatgaggtg gcgctcgacg aggaagtcgc ggtcgagggg gctgatgtcg ccggcgcgga
     1141 cgtagcggcc gcggccgaga aggtcggagc gggagacggt ggagtcgaca tagttcacga
     1201 tgcgggtgcc ggtttcgctg tcggccgagg ggggatattt gcagccggcg atgttgcggg
     1261 ccaggcgaac ccggctggac agcacggcgg ccgattcctc gcccttgccc gacagccaga
     1321 cggccggtga cttgaccata tcttcaaaca tcgtccatct tccccatctc cccccgcggc
     1381 taccggtgaa gggatgattc ctcgcggatc gttttgagtt tgtcgcggat ttcagcggcc
     1441 cgctcgaaat cttcgctctc gatagctttg gccagctccg cctcgaggcg ctcctcctct
     1501 ttgacgggga tggcatcatc gtccgccgcg ccgtcgatga cggtgacggg gcgctggccg
     1561 cggtggaggg aggcgccgtg gattttgcgc atgatcggtt cgaggcggga gcggaaagct
     1621 ttgtagcatt cgccgcagcc gaagcggccc tggcgggtga aagcctggaa ggtgaggccg
     1681 cagccggggc aggtgagcgg gtcgccggtg tcgaccggcg gcgcgccctg gggggaggag
     1741 gcgaagccgg agaggatttc ggccaggggg aagggaacgt tttccagggg ggagtggaag
     1801 ccgcgggcgg ccgcgcactg cttgcacagg ttcagcatcg tcttctggtt gttgacaatc
     1861 tgcgtgaggt gaaccgtcgc ctcgttcttg ttgcagtcct gacacaacat agtcacattc
     1921 ccatgcggtt acgagcgctc gcagaacttg ccttcgacga tctccaaagt gcggttgcag
     1981 cggtcggcca gttcgcgatt gtgggtcgcg atgaggaggg cgatgctctg ttcgcgattg
     2041 aggcggaaga gcagttcgtg aagcatccgt cccgttgcac tgtcgaggtc gccggagggt
     2101 tcgtcggcca gaacgatccg gggatcgttg gccagggccc gggcgaccgc cacccgttgc
     2161 tgttcaccgc cggagagctg cgccgggcgg tggttctttc tctcacttaa accgaccatg
     2221 tcgagcaaca gttccgcccg ccggcgggcc tgggtgcggc tctggccggc gacgaggacg
     2281 ggcatcatga cgttttcggc cgccgagaaa tcctccagca agtagtgaaa ctggaacacg
     2341 aaaccgacat tgcggttgcg gaacgccgcc aagtccgcct ccgactggtt gtccacccgg
     2401 gtcccgtcga tctcgactgc cccggccgtc ggccgatcca ggccgccgag gaggtgcagg
     2461 agggtcgatt tgccgacccc ggaggcgccg gtgacagcca cggtgtcgcc gcgaggcatg
     2521 gtcaggttga tgccgcgcag gacgtgcagg gtcgcgtccg gggtccggaa ctcgcggtgg
     2581 aggtcaacgg ctcgcaagat ggtgtcgtcg tgggtgtcca tcgttcggtt tactcgtcct
     2641 cttgggcccc gttacgggca tacagtagta ttttcgccag aattccgtaa acgtttacca
     2701 ttgcgcgtgt tttccgccat ttagcgccgc aaaacctcca cgaccgacac ccgcgaggcc
     2761 tggtgggccg gatacagggc ggccaaaaag cagatcgcca ccgtcaccag gccggccagc
     2821 aggaaatcga ccgggtggat atcgatcggg aggtagctga tgaaatagat gtcggggggc
     2881 aggctgacca gatggtaccg gttttgcagc caggcagccg ccagcgccag cccccagccg
     2941 cccgcgacgc cccccgcgcc gatccctaag cccttgtaca cgaatatctt gcggatcgat
     3001 ccgggagtcg aaccgatcgt tttcagaatg ccgatttcag cccgcttctc catcgtcaac
     3061 ataaccagtg tcgagatgat ggaaaaagcg gcaaccagca cgatcagaat gaaccccagg
     3121 aagagcacct tcttctcgat ggcgatccag gcgaacagat tccggtggag gacgttccat
     3181 gggaccactt cgaaacggtg ctcgaggagt gaatcgacga ccgggcggat cgcctcggcc
     3241 cggtagacgt cgtcgaggcg caggtggatg gtggtgacgg cgtcgccggt acggaagagt
     3301 ctctgggccg cgggcagcga gatgtaggcg agctcggagt caaactcgaa catgccgctt
     3361 tcgaagatgc cggcgacata gaacttggcg acccgcgggc gggtgttgct gcggatttca
     3421 tcgcccttga gggagtagag gacaacctcg gagccgagga cggcgcccag acggtcggcg
     3481 agattcttgc cgaggatgat gcccggcacg gtgtcgtcgg cgatgacgat ctcgttgaaa
     3541 ttgtaggttc ccccgacgat gttcttttcg atctgggagg cgcggcgctc gagttcgggg
     3601 acgatgccgc gcacgatgat gccgtcaccg gcgctttcgg aagcgatggc ggccttgtag
     3661 tagatgaagg gcgaggcggc ggcgacgccg ggaacgcggg cgagggaatc gaccagggcg
     3721 cggtaattcg cgatgaactc ctcccgaacc gggaagatcg agatgtggga ggtggtgccg
     3781 agcagacggg tgcggatctc ggcctcgaac ccgttgtgca tggacatgac gaaacagacg
     3841 acgcctacgc cgagggccac cccgaggatc gtgatccagg tcgagacgga gatgaagaac
     3901 cgccccgacc gaagatatcg tcgcgccaca aaacgttcat agcccatgca tccatccatc
     3961 cctggtgcaa aaccggcacg gcactcattg tgaaggccgg tgcgcagcgg cactcagggt
     4021 attatacgcc ccgccggagg caacgcaatc tttttcggac cggcggacca tgcggccccc
     4081 ttgacagcag acggagcaca tgcgaagttg aggccg
//
LOCUS       DAOOKS010000072         4101 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_26280_length_4101_cov_2.860850, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000072 DAOOKS010000000
VERSION     DAOOKS010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4101
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26280_length_4101_cov_2.860850"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1797)
                     /gene="aspS"
                     /locus_tag="PK112_01895"
     CDS             complement(<1..1797)
                     /gene="aspS"
                     /locus_tag="PK112_01895"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011877131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HPC10832.1"
                     /translation="MVKFSEVKRTHSCGELRPAHVGTTVRLNGWVNSYRNLGGLFFLD
                     LRDRYGITQVVINPETFDKDMLAEAQRVRHEYVVAVTGKVRHRPEGTANERMLTGGIE
                     VVAEAFWILSESKTPPFEIEEETSASEKLRLEYRYLDLRRGPLQERIRFRHDLTMCVR
                     KYMSAQDFLEIETPLLIRSTPEGARDYVVPSRVHHGRFYALPQSPQLFKQILMIAGFD
                     KYFQIAPCLRDEDLRSDRQPEHTQIDVELSFATPEDVFAVIEGLMANVFENLAGAAVA
                     TPFPRYTYAEAISRWGIDKPDLRFGMEIVDLTEIAGASEFKVFRENAARGGVIKGIVL
                     SGGAAYSRKQLDELTELAKQQGAGGLAYILRTAEGDKSPILKFLGEATAAAMSAQAKA
                     ATGDALFLVSDRPAKTEAILGQLRLHLGRAHRLAARNEWRFLWVVDFPLFEFDEESRT
                     LQAAHNIVSHPMEEDVPLIEAGFASPLPVSDPGHPWRRARAMQYDLVLNGWEIASGGQ
                     RINRRDLQQRILNILGIDDERAERMFGFLLRALEYGAPPHAGIAAGLDRLAALMTGGE
                     SIRDVIAFPKTTNAMSLMDGSPAKIDPHQLAEL"
     gene            2194..2694
                     /locus_tag="PK112_01900"
     CDS             2194..2694
                     /locus_tag="PK112_01900"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HPC10833.1"
                     /translation="MNISRYMTEDAVKLEMETVIDPPEEGQSIERWREQAKSLILLEL
                     VDLLNETHRVGNRNKLLIDFVNREKKASTAIGHGIAFPHIRSMQATEFMIAFARSHTG
                     YDYGAPDSAPVRLFFIMAAPPYEDNLYLKVFKALAEMLQYESFREELLSVTSPGEVLR
                     AIRAME"
     gene            complement(2773..>4101)
                     /locus_tag="PK112_01905"
     CDS             complement(2773..>4101)
                     /locus_tag="PK112_01905"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
                     /protein_id="HPC10834.1"
                     /translation="ELPVGGVTMLSWAGSYKIVLASFGLEGVADGYVANGYATKQMVL
                     QRVLDFFAADSVSQNPSITAVAVGGGTSPLRLVDHTPEFSWSVVDTAGGPAVAYEVQV
                     GTGDLCFNADDMWSPGAAAGADTSAVYAGAPLADGQDYVFRVRVSNGASWSEWERLEF
                     GMNAVSASGLLVAPAQDSLVTTATPTLKVTNTTDPDGDVQTYEFAVYADQELTTVVAA
                     AAGVTQAPPHTVWTVSTPLADDARYWWRARAWDGYEYSPWSAVGTFLVNAVNQAPAAF
                     SLLAPANGDTVAVHPTFVWQASTDGDLGDAVSYTLKYSEDSLFALYDYVQLAETTVVL
                     FSPLDEGTRYFWKVEARDRFSGMTVSTETFRLTTQTTGCCTRRGDYDNDGSIKVSDVT
                     LLVGYLFRGGVPPVCAEHGDANGDGEIKVSDLTLLVGYLFRGGAAPAPCP"
BASE COUNT          724 a         1430 c         1249 g          698 t
ORIGIN      
        1 cagttccgcc aactggtgtg ggtcgatctt ggccggcgaa ccgtccatca gcgacatcgc
       61 gttggtcgtc ttgggaaagg cgatcacgtc gcggatggac tcgcccccgg tcatgagagc
      121 ggccagccgg tcgagaccgg ccgcgatgcc ggcgtggggg ggcgcaccgt actcaagcgc
      181 ccgcaggaga aatccgaaca tccgctcggc ccgctcgtcg tcgatcccca ggatgttgag
      241 gatccgctgc tggagatccc gccggttgat ccgctgaccg ccggaggcga tttcccagcc
      301 gttgagcacc aggtcgtact gcatcgcccg ggcccgccgc cacggatggc cggggtcgga
      361 caccggcagc gggctggcaa acccggcctc gatcagcggc acatcctctt ccatcgggtg
      421 cgatacgata ttgtgcgccg cctgtagggt gcgcgactcc tcatcgaatt caaacagcgg
      481 gaaatccacg acccacagga accgccactc gttccgggcc gccagccggt gcgcccgccc
      541 caggtgcagc cgcaactgcc cgaggatcgc ttcagtcttc gccgggcggt cggagaccag
      601 gaacagggca tcgccggtcg cggctttggc ctgggcgctc atggccgccg ccgtcgcctc
      661 gcccaggaat ttcaggatcg gcgatttgtc gccttccgcg gtcctcagaa tatacgcaag
      721 ccccccggcc ccttgttgct tcgccagctc ggtcaactcg tcgagctgtt tgcgcgagta
      781 tgcggctccg cccgacagca cgatcccctt gatcacgccg ccgcgcgccg cgttctcccg
      841 gaacaccttg aattccgacg cccccgcgat ctcggtgagg tcgacaatct ccatgccaaa
      901 gcggaggtcg ggtttgtcga ttccccagcg gctgatggcc tcggcatagg tgtagcgcgg
      961 aaacggcgtc gccacggccg cccctgccag gttctcgaac acgttcgcca tcaggccctc
     1021 gatgaccgcg aacacgtcct cgggcgtcgc gaacgagagc tcgacgtcga tctgcgtgtg
     1081 ttcgggctgg cggtcgctgc gcagatcctc gtcgcgcagg cacggggcga tctggaaata
     1141 cttgtcgaag cccgcgatca tgaggatctg cttgaacaac tgcggcgact gcggaagggc
     1201 gtagaagcgg ccgtggtgga cgcggctcgg caccacgtag tcgcgcgccc cttccggcgt
     1261 cgagcgaatc agcaggggag tctcgatctc caggaagtcc tgcgcgctca tgtatttgcg
     1321 cacacacatg gtcagatcat ggcggaaccg gatgcgctcc tgcagcggcc cccgccgcaa
     1381 atccagatac cggtactcca ggcgcagctt ctcgctcgcg ctcgtctcct cctcaatctc
     1441 aaacggcggc gtcttcgatt ccgaaaggat ccagaaggcc tcggccacca cctcgatccc
     1501 gcccgtgagc atgcgctcgt tcgccgtccc ctccgggcgg tgccgcactt tccccgtgac
     1561 cgccacgacg tactcatggc ggacacgctg cgcctcggcc agcatgtcct tgtcgaacgt
     1621 ttccgggttg atcaccacct gggtgatgcc gtagcggtcg cgcaggtcca gaaaaaacag
     1681 cccgcccagg tttcggtaac tgttgaccca tccgttaagc ctgacggttg tgccgacatg
     1741 agcggggcgc aattccccac aggagtgggt gcgtttcact tccgaaaatt tgaccaccag
     1801 agaaatttaa ctccttgctg tcaaaatatt tatcggcacg ttcgggccca aggttcaggc
     1861 cgccggcgcc gctgacgtac cgggaaagta cactcccccc gaccgaatgt caaggttgcc
     1921 ggttccccga ccggacagac ccggcggccg ccgcccctcc ctatggtgcc gccacggacc
     1981 ttatgcccgg cgacatcgga ttgacaggga gccggcacct gtcggtggca tcaattgatt
     2041 tgcccggcct caagcaggta actggcccgg tgacggcctg agtcaccggc agcgtcgcac
     2101 tccggggtac ggagggatct cctgttgaca atctcgcagg ccgtcacgta tactctcctt
     2161 catggttaac cggttgcgac cgatagaatt actatgaata tctcgcgcta catgacggaa
     2221 gatgcggtca aactggaaat ggagaccgtc atcgatccgc ccgaggaggg ccagtcgatt
     2281 gaacgctggc gggagcaggc caagtccctg atcctcctcg agctggtcga cctcctgaac
     2341 gagactcacc gcgtcggcaa ccgcaacaag ctcctcatcg actttgtcaa ccgcgagaaa
     2401 aaagcttcga ccgcgatcgg ccacggcatc gcctttcccc acattcgctc catgcaggcc
     2461 accgagttca tgatcgcgtt tgcccgttcc cataccggct acgactacgg cgctcccgac
     2521 tcggcgccgg tccgcctttt tttcatcatg gccgccccgc cctacgaaga caacctctac
     2581 ctcaaggtct tcaaggccct cgcggagatg ctgcagtacg agtcatttcg cgaggagctg
     2641 ctcagcgtta cctcgcccgg cgaagttctc cgcgccatcc gcgcgatgga gtgagaatcg
     2701 gcgggcatca ccggcaagag aaaaccgggc cccgagggac ccggttcggc aaaagtcaaa
     2761 ctggcgcaag gctcacggac acggcgccgg cgccgccccg ccccggaaga ggtagccgac
     2821 caggagcgtc aggtccgaca ccttgatctc accgtccccg ttggcgtccc cgtgctcggc
     2881 gcagaccggc ggcacgcccc cgcggaagag gtagccgacc agcagcgtca catcgctgac
     2941 cttgattgaa ccgtcgttat cgtagtcgcc gcgccgggtg cagcagcccg tcgtctgggt
     3001 ggttaggcga aaagtctcgg ttgacaccgt catgcccgag aagcggtcgc gggcttccac
     3061 tttccagaaa taccgcgtgc cttcgtcgag cgggctgaac agcacgactg tggtctcggc
     3121 cagctgcacg taatcataca gggcgaacag cgaatcctcc gaatacttga gcgtgtacga
     3181 gacggcgtcg cccaggtcgc cgtcggtcga ggcctgccag acaaaggtcg ggtgcacggc
     3241 gaccgtgtct ccgttggccg gcgcgagaag cgagaaggcc gccggggcct ggttcacggc
     3301 gttgacgagg aatgtcccca cggccgacca cgggctgtac tcgtagccgt cccaggcccg
     3361 cgcccgccac cagtatcggg cgtcgtccgc tagcggcgtc gacaccgtcc acaccgtgtg
     3421 cgggggcgcc tgcgtgaccc cggcggcggc cgccaccacc gtcgtcaatt cctggtcggc
     3481 gtacacggcg aactcgtacg tctgcacgtc cccatcgggg tcggtggtgt tggtgacctt
     3541 gagcgtaggc gtcgccgtcg tcaccagcga atcctgcgcc ggcgccacga ggagtcccga
     3601 ggcgctcacg gcgttcattc cgaactccag gcgttcccac tcggaccagc tcgcgccatt
     3661 ggacacgcgc acccggaaga cataatcctg gccgtccgcc agcggcgcgc ccgcgtacac
     3721 agccgaggtg tcggcgccgg cggccgcccc cggcgaccac atgtcatcgg cgttgaaaca
     3781 gagatccccc gtccccacct ggacctcgta tgcgaccgcc gggcccccgg ccgtgtcgac
     3841 cacgctccac gagaactccg gcgtgtggtc gaccagccgg agcggcgagg ttccgccgcc
     3901 caccgccacc gccgtgatcg acggattctg cgacacggag tcggcggcaa agaagtcgag
     3961 cacccgctgc agcaccatct gcttcgtcgc gtaaccgttg gcgacatacc cgtcggcgac
     4021 gccctcaaga ccgaagctcg ccagcacgat cttgtaggac cccgcccagg agagcatcgt
     4081 cacgccgccc accggcagct c
//
LOCUS       DAOOKS010000073         4090 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_26366_length_4090_cov_3.560843, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000073 DAOOKS010000000
VERSION     DAOOKS010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4090
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26366_length_4090_cov_3.560843"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="PK112_01910"
     CDS             <1..441
                     /locus_tag="PK112_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10835.1"
                     /translation="VSAPPTPPIDPQRTLDLAAVRAGWEGFLAELRQRKAMLSSQLAM
                     ATLVEVKDNELAVVFGAGGTMPLQIVTRTENLHVITTALREHYRAPLRIKFSLDPRRQ
                     GPGPTEEKRGLDRAQIEKLIADSPRIRRLLDLVEGEVIGVRKVT"
     gene            466..792
                     /locus_tag="PK112_01915"
     CDS             466..792
                     /locus_tag="PK112_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HPC10836.1"
                     /translation="MRGGLGDMMKQVQQMQAKMAALQEELENTELEASSGGGMVKVVM
                     NGKNVVRSITIDREVVDPEDVEMLQDLIVAAVNQAREKVQELQAQQMAQITGGLNLPG
                     LGGLPF"
     gene            815..1411
                     /gene="recR"
                     /locus_tag="PK112_01920"
     CDS             815..1411
                     /gene="recR"
                     /locus_tag="PK112_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017620501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HPC10837.1"
                     /translation="MFKSAASVERLTNRLARLPGIGRKSAARLAFHLLKLPREEALEL
                     ADAIREVKDKVGFCSVCNNISETDPCQICSDPRRRRSLICVVEETADAAAIDRVEGFD
                     GVFHILGGRLSPLDGIGPDDLKIKELLARIGGGDTEVVIATNPNVEGEATAMYLSRLL
                     KPMGVRVTRIARGLPVGSDLEYADAVTLARALDGRQEF"
     gene            1422..3455
                     /locus_tag="PK112_01925"
     CDS             1422..3455
                     /locus_tag="PK112_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10838.1"
                     /translation="MHRTGTHSQLGLLAGLTVLLFVAGRLASGALGPNNWSFTHWQAL
                     ARPSAAAWLAVALVLGVALGRFLPAIAAFLQPRRKAAVGATLLIVLIVLLRCDSFVAG
                     GGNLRIAQIAQTADVILPWYEYGAVAAVRLFYRCGVIAGLPEAAAAAAAWQVFGFACT
                     ILALAAAWRLARLLSDDPTRRLGLFVIVFFGPQTAVYFGFIGPVPVIAAAALWLAYAL
                     VRAAEKPSPRRLGLVWGVFALSVAMHAAMVFALPAVVAGTVGGLRRRGRPGVAPFLAG
                     AVVWIGLPAALYLVANEYLPLKASLLLAAGKPPHGDYSLFSPRRLGDLGQVLFLLFPL
                     GLVALYALIRWTRRAVTDLACSTALLLFLGGLSAVFVIDPVHSIVLDLPLYAPFLAPG
                     AVFLAVSLRATSGSPSISPSALGMIAAASLMLPAGYLPVYTRLATVERYITPYLDRHS
                     AYYRTAAVALRDAYFYRKDLERANYWDQSYRRKSPDYLNLTGIEDFILAQRLDIALPS
                     LNKLIARNPYWAEPRAVYGKVMTEQRRFDLGKPQLDTALMLEPYNPRRHMNLYGYYRD
                     KGEFAQALTAVTRAAELFPNNDEIRTDLMIISYRAGHGERARELAEHLLRLDSTRAYP
                     YAIMGFLAEEAGDLSSAMANFERFCSLAPEAPETPQIRKRANAIYLKLNPAAP"
     gene            3587..4048
                     /locus_tag="PK112_01930"
     CDS             3587..4048
                     /locus_tag="PK112_01930"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HPC10839.1"
                     /translation="MQKSEQPGRFRYAGLGRRFAALMLDFALGSLVFFPVTKIAKGVW
                     ILRPEEHAWRYGWLITDPLCLMFLAVILAYFILSEGLLGATLGKRLVAIRVVRVNGER
                     PGLGRSLIRNVLRAVDALPAFNILGIVLIVSSPEKARAGDRVTGTRVIALR"
BASE COUNT          631 a         1354 c         1377 g          728 t
ORIGIN      
        1 gtaagcgctc cgccgacgcc gcccatcgat ccccagcgaa cgctcgacct ggccgcagtc
       61 cgggccgggt gggagggctt cctggccgaa ctccggcagc gcaaagccat gctctcgtcg
      121 cagttggcca tggcgacgct ggtcgaggtc aaggacaacg agctggccgt ggtgttcggc
      181 gccggtggaa ccatgccctt gcagatcgtg acgcggaccg agaaccttca cgtgatcacg
      241 accgcgctgc gagaacacta ccgcgcgccg ctgcgtataa agttcagtct cgacccgcgg
      301 cgccagggcc cgggacccac ggaagagaag cgcggactcg accgcgcgca gatagagaaa
      361 ctgatcgccg actcaccccg catccgcagg ctcctcgacc tcgtcgaagg ggaagttatc
      421 ggcgtgcgaa aagtaacctg agcgacccga aaggatagca ccaccatgag aggcggactg
      481 ggcgacatga tgaagcaggt tcagcaaatg caggccaaga tggccgcgct gcaggaagag
      541 ctggaaaaca ccgaactcga ggcctcgtcg ggcggcggga tggtcaaggt tgtcatgaac
      601 ggcaagaacg tggtccgctc gatcaccatc gaccgggaag tggtcgatcc cgaggacgtg
      661 gagatgctcc aggatctcat cgtggcggcg gtcaaccagg cgcgcgagaa ggtgcaggaa
      721 ctgcaggcgc agcagatggc gcagatcacc ggcgggctga atctcccggg cctcggcggc
      781 ctgcccttct gagcgggacg gaggccggac acccatgttc aaatccgcag catcagttga
      841 gcggctcacc aaccgcctgg cccgccttcc cgggatcggc cggaaatccg cggcccgcct
      901 cgccttccac ctgctcaaac ttccccgcga ggaggcgctg gagctcgccg acgccattcg
      961 cgaggtcaag gacaaggtcg ggttctgctc ggtctgcaac aacatctcgg agaccgatcc
     1021 ctgccagatc tgctcggatc cgcggcggcg ccgcagcctc atctgtgtgg tcgaagagac
     1081 cgccgatgcc gccgcgatcg accgggtcga ggggttcgac ggggtcttcc acatcctcgg
     1141 cggacggctc tcgcctttgg acggtatcgg gccggacgac ctcaagatca aagagttgct
     1201 cgcccgcatc gggggcgggg acacggaagt ggtgatcgcc accaacccga atgtcgaggg
     1261 ggaggcgacc gccatgtacc tctcccgcct gctgaagccg atgggggtgc gggtcacgcg
     1321 gatcgcgcgg gggctgccgg tcggctcgga cctcgaatat gccgatgccg tcacgctcgc
     1381 acgggcgctc gatgggcggc aggaattctg attggccggc gatgcaccgc accgggacac
     1441 atagtcaact gggacttctc gccggcctga ccgtgctcct gtttgtggcg gggcgtctgg
     1501 cgagcggggc gctggggccg aacaactggt cgtttacgca ctggcaggcg ttggcgcgtc
     1561 cctcggccgc cgcctggctc gcggtcgcgc tggtgctcgg cgtggctctg ggccggttcc
     1621 tgccggcgat cgccgctttc ctccagcccc ggcgcaaggc cgcggtcggc gccacgctgc
     1681 tgatcgtact catcgtcctt ctccgctgcg attcattcgt cgccggaggg ggaaacctcc
     1741 gcattgccca gatcgcccag accgcggacg tgatcctccc gtggtacgag tacggcgccg
     1801 tggccgcggt ccggctgttc taccggtgcg gcgtgattgc cggacttccg gaggcggcgg
     1861 ccgccgccgc agcctggcag gtgttcggtt tcgcctgcac aattctcgcg ctcgcggccg
     1921 cctggcggct ggcccggctg ctttcggacg atcccacgcg ccgcctggga ttgttcgtca
     1981 tcgtcttttt cggtccgcag acggcggtgt atttcggctt catcggcccg gtgccggtga
     2041 tcgccgccgc cgcgctctgg ctggcgtatg cgctggtccg ggcggcggaa aagccctccc
     2101 cccgccgact cggcctggtc tggggcgttt tcgccctgag tgtcgctatg cacgcggcga
     2161 tggttttcgc gctcccggcc gtggtcgccg gcacggttgg cgggctgcgc cgacgggggc
     2221 ggccgggcgt cgcgccgttt ctcgccggag cggtcgtgtg gatcgggctg ccggccgcgc
     2281 tctaccttgt ggcgaatgag tacctcccgc tgaaagcgtc gctgctgctg gcggcgggca
     2341 agccgccgca cggggattat tcgctgttct cgccgcggcg actcggcgac ctgggccagg
     2401 tgctgttcct gttgtttccg ctgggtctgg tcgccctgta cgcgctgatc cggtggacgc
     2461 gccgggcggt gaccgacctc gcctgttcga ccgcgctcct cctgttcctg gggggactca
     2521 gcgccgtttt cgtcatcgac ccggtgcaca gcattgtgct ggatcttccc ctgtacgctc
     2581 ccttcctggc gccgggcgcg gtgtttctcg cggtgagcct gcgggcgact tcgggcagtc
     2641 cgagcatctc tccatccgca ctcgggatga ttgccgccgc ctcgctcatg ttgccggccg
     2701 gatacctacc cgtctacacc cgcctcgcga cggtcgagag gtacattacc ccctacctgg
     2761 accggcacag cgcgtactat cggacagccg ccgtggccct gcgggacgcg tatttctatc
     2821 ggaaggatct ggagcgcgcg aactactggg accagagcta ccggcggaaa tccccggact
     2881 acctcaatct caccggcatc gaagatttca ttctggccca gcgccttgac atcgccctcc
     2941 cgagcctgaa taaactgatc gcccgcaacc cgtactgggc ggaaccgcgc gccgtgtacg
     3001 ggaaagtgat gaccgagcag aggcgtttcg atttgggcaa gccgcagctc gacacggcgc
     3061 tgatgctcga gccgtacaat ccgcggcgcc acatgaacct gtacggctac taccgggaca
     3121 agggggagtt tgcccaggcg ttgacggcgg tcacccgcgc ggcggagttg tttccgaaca
     3181 acgatgagat ccgcaccgac ctgatgatca tcagctaccg cgccgggcac ggcgagcgcg
     3241 cgcgggagct ggccgagcac ctgctgcggc tggactcgac gcgggcctat ccgtacgcca
     3301 tcatgggttt tttggcggag gaggcgggcg acctgagctc ggccatggcc aatttcgagc
     3361 gcttctgcag tctcgcaccc gaggctccgg aaacgcccca gatccgcaaa cgcgccaacg
     3421 cgatctatct caagcttaat ccggccgccc cgtgaacccc tccgcagggg catggcggct
     3481 ccacccctgg gtcggccgcc gggccgtcga gcggtacgac gaaacagcac ttggaccgaa
     3541 gcggtccggg tgatagattg ccccggagtc tcccgagggt aaccgcatgc agaagagcga
     3601 acaacctggc cgctttcgtt acgccggcct ggggcgccgc ttcgcggccc tcatgctcga
     3661 cttcgcgttg ggcagcctgg tcttcttccc cgtcaccaag atcgcgaagg gggtctggat
     3721 cctgagaccg gaggaacacg cctggcgata cgggtggttg atcaccgatc ccctgtgcct
     3781 aatgtttctc gcggtgattt tggcctactt cattctctcg gagggcctgc tgggcgcgac
     3841 gctgggcaag cggctggtgg ccattcgcgt tgtccgggtg aacggggagc gaccggggct
     3901 gggccgttcc ctgatccgca atgtcctgcg ggccgtggat gctctccccg cgttcaacat
     3961 tctcggcatc gtgctgattg tttcctcgcc ggaaaaagcg cgtgcgggcg atcgcgtgac
     4021 cgggacgcgg gtgatcgctc tgaggtaatt gggagaggtc gctctgcgct caggtgacgc
     4081 cagtccggcg
//
LOCUS       DAOOKS010000074         4065 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_26584_length_4065_cov_3.597506, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000074 DAOOKS010000000
VERSION     DAOOKS010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4065
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26584_length_4065_cov_3.597506"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1677)
                     /gene="dnaG"
                     /locus_tag="PK112_01935"
     CDS             complement(<1..1677)
                     /gene="dnaG"
                     /locus_tag="PK112_01935"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HPC10840.1"
                     /translation="MIAPDTIEQVRQATDIVALIGQYVRLKRQGRRLVAVCPFHTEKT
                     PSFSVSPDRQMYYCFGCQRGGTVFTFLQEHEGMSFVEAVQFLAQKANIHIREDRERDG
                     RREASEKIAYANSVALEFFQKQLFNPRYRAALEDYLMKRRGITRDTIREFQLGLAGEE
                     WDSLLRFARPKGLSPEDLVECGLALYSDKSRQHYDRFRQRLMIPIFNLSQKPIAFGGR
                     TLKKGETVKYMNSPETALYSKGSVLYNLNFARDPIRSENAVFVVEGYFDVISLWQAGV
                     KNVVASSGTAFSLPQARLLARFAESVYLFFDADSAGRAAALRSVDVLYDAGLEVKVVV
                     APPGEDPDSVARAHGREKIEQLRRAAVGYITYRTQDIDVRRAGIIGREKLVKEFAALG
                     ARIADPTRRSLFWEEAATVLGLNPDLLISAAPREAAAAETAPAKRARKYDPLALHLLS
                     LMLHNPGAIDAVFERVAPEDFEAPELTRLYAAMARQYRVTGEIDARGLVDTAQDEAFQ
                     SLIAAVAAIEWEPDKVEAEARKMLKEFLTQRSRASLPELKRQLAEAEAAGD"
     gene            complement(1681..>4065)
                     /locus_tag="PK112_01940"
     CDS             complement(1681..>4065)
                     /locus_tag="PK112_01940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HPC10841.1"
                     /translation="CRRRREREMIDQHTLDILEYPKVIAQVAGRCLTPYGADEVNRIR
                     PLYDPAEIERRGSEISQMRDIVSFGMAFPLARMENCRPELLRAQVEGVFLDPEQILRV
                     LELVEVSIALNGYDREGRPKFPLIAAYLEQVRAFPELKAEIRRAIDENGEVKDSASPK
                     LKQVRAEFGDAKRRLVARLTAIQADRPHQAGVQDDIVTMRNGRYVITVPSSSYRANMG
                     ILHDRSQTGATLYIEPPETVELNNRLNLLQQEERLEIDRILRAITAEIARRGEALLEN
                     TRLIGRLDSLYACAKYSKAVGGNKPALGPESSLSLVDARHPLLAVQFGKPEAVVANSL
                     ALDDLRQAILVTGPNTGGKTICLKTVGLAVLMAQSGLHIAAADKSSVGIFEYVCADIG
                     DEQSIELSLSTFSSHVRNLIDGVRKASPRTLLLFDEIGAGTDPKEGAALAEAIILHAI
                     RKGARMIVTTHFSQLKTLPMDNPEIENASFEFDRTTLAPTYRLQLGTPGSSYAIEIAS
                     RLGMSEEITERASRLVGSGEKSLAALISSLESELAVVRKDRAELTDRLAEARRLEDTY
                     RSRVDELTTNVEETRRRALAETEAFIEQTRREVEQLVADIRRSQAEPQQVKNFHRSLR
                     ERAKELSSLKKASETPAPEPGRFEKGDRVEIVSLGQAGEIDELLGDDRARVKVRNVFT
                     TVEIRHLRRLEEGGAPARPEATGARVSARPVESPEIHLRGLTGDEAIEELERYLDRAV
                     VAGLHQVYVVHGKGTGALRKRITEYLREHPEVASVRLGNWNEGGAGVTIVKLKE"
BASE COUNT          623 a         1281 c         1425 g          736 t
ORIGIN      
        1 gtccccggcc gcttccgcct cggcgagctg ccgtttcagt tcgggaaggc tcgcgcggct
       61 tctctgcgtg aggaactcct tcagcatctt gcgggcctcg gcctccactt tgtcgggctc
      121 ccattcgatg gcggcgaccg cagcgatgag cgactgaaag gcctcgtcct gcgcggtgtc
      181 gaccagtccg cgggcgtcga tctcgccggt cacgcggtac tgccgggcca tggcggcata
      241 gagccgggtc agttcggggg cctcgaaatc ctccggggcc acgcgttcga aaaccgcgtc
      301 aatggcgccc gggttatgga gcatgaggga gagcaggtgg agggcgagtg gatcgtattt
      361 ccgcgcccgc ttggccggcg cggtctcggc ggcagccgcc tcccgcggag ccgccgaaat
      421 gagcaggtcg ggattgaggc cgagcaccgt ggccgcctcc tcccagaaga ggctgcggcg
      481 ggtgggatcg gcgatgcgcg ctccgagggc ggcgaattct ttgaccagct tctcccgccc
      541 gatgatgccc gcgcggcgca cgtcgatatc ctgcgttcga tatgtgatgt atccaaccgc
      601 ggcgcggcgg agctgctcga ttttctcgcg gccgtgcgcg cgcgccaccg aatcggggtc
      661 ctcgcccggc ggcgccacca caaccttcac ctccagcccc gcgtcgtaga gcacgtcgac
      721 cgagcggagg gccgcagccc ggccggccga gtcggcgtcg aaaaagagat agacgctctc
      781 cgcaaagcgg gccaaaagcc gggcctgcgg caagctgaaa gcggttcccg acgaggccac
      841 gacgttcttc acgcccgcct gccagaggga gatgacgtcg aagtagccct cgactacgaa
      901 gacggcgttt tcgctgcgaa tgggatcgcg ggcgaaattg agattgtaca ggacactgcc
      961 cttggagtag agcgccgtct ccggcgaatt catgtacttg accgtctcgc ccttcttcag
     1021 cgttcgcccg ccgaacgcaa tcggcttctg gctcaggttg aaaatgggga tcatgaggcg
     1081 ctggcgaaaa cggtcgtagt gctggcggga cttgtcggaa tacagggcca gtccgcattc
     1141 gacgagatcc tcgggggaga ggcctttggg acgggcgaaa cggaggaggc tgtcccactc
     1201 ttcgccggcc aggccgagct ggaactcgcg gatggtatcg cgcgtgatgc cgcggcgctt
     1261 catcagatag tcctcgagcg ccgcccggta ccgcggatta aacaactgct tctggaagaa
     1321 ctcgagggcg acgctgttgg cgtaggcgat cttctcgctc gcctcccggc ggccgtcgcg
     1381 ctcgcggtcc tcgcggatgt gaatgttggc tttctgggcg aggaactgta cggcctcgac
     1441 gaacgacatc ccctcgtgct cctggagaaa ggtgaacacc gtcccccccc gctggcagcc
     1501 gaagcagtag tacatctgcc ggtcggggga gacggagaag gagggggttt tctcggtgtg
     1561 gaaggggcag acggcgacca ggcgccgtcc ctggcgcttg aggcggacgt actggccgat
     1621 gagagcgacg atatcggtgg cctggcggac ctgctcgatt gtgtcgggcg caatcatggg
     1681 ctactctttg agcttgacga tggtgacccc ggcgccgcct tcgttccagt tgccgagccg
     1741 cacggaagcg acctccggat gttcccggag atactcggtg atgcgtttgc gcagggcgcc
     1801 ggtccccttg ccgtgcacga catacacctg gtgcagtccg gccacgaccg cccggtcgag
     1861 gtagcgttcg agttcttcga tggcctcgtc gccggtcagg ccgcgcaggt ggatctcggg
     1921 ggactcgacc ggccgggccg acacccgcgc gcccgtcgcc tccggccgcg ccggcgcacc
     1981 gccctcttcg agccggcgga gatggcggat ctcgacggtg gtgaagacat tccgcacctt
     2041 cacccgggcc cggtcgtcgc cgaggagttc gtcaatctcg ccggcctggc cgagggagac
     2101 aatctcgacg cggtcgccct tttcgaaccg gcccggctcc ggcgccgggg tctccgaggc
     2161 tttcttcagc gacgaaagtt ccttggcccg ctcgcgcagg gagcggtgaa agttcttgac
     2221 ctgttgcggc tcggcctgcg agcggcggat atcggccacc agctgctcga cctcgcggcg
     2281 ggtttgctcg atgaaagcct ccgtctcggc gagcgccctg cggcgcgttt cctccacatt
     2341 ggtcgtgagt tcatccacgc ggctcctgta ggtgtcctcg aggcggcggg cctcggcgag
     2401 ccggtcggtc agttcggcgc ggtctttgcg gacgacggcc agctcgctct ccagcgagga
     2461 gatgagcgcg gccagcgatt tttcccccga cccgacgagc cgggaggcgc gctcggtgat
     2521 ctcctcggac atgcccagcc ggctggcgat ttcgatggcg taggacgacc ccggcgtgcc
     2581 gagctggagg cggtaggtcg gggcgagcgt cgtgcggtcg aactcgaacg aggcgttctc
     2641 gatttcaggg ttgtccatcg ggagcgtctt gagttgcgag aagtgggtgg tgacgatcat
     2701 gcgggccccc ttgcggatcg cgtgcaggat gatcgcctcg gccagggcgg ccccctcctt
     2761 ggggtccgtg ccggcgccga tttcatcgaa cagcagcagc gtgcgtggcg aggccttgcg
     2821 gacgccgtcg atgaggttgc ggacgtggga ggagaaggtc gacagggaca gctcgatgga
     2881 ctgctcgtcg ccgatgtcgg cgcagacata ttcgaaaatg ccgaccgagg atttatccgc
     2941 ggcggcaatg tggagccccg attgcgccat gagcacggcc aacccgaccg tcttcaggca
     3001 aattgtcttt ccgcccgtgt tgggcccggt cacgaggatt gcctggcgga ggtcatcgag
     3061 ggccaggctg ttggcgacca cggcctcggg cttgccgaac tggaccgcga ggagcgggtg
     3121 gcgggcatcg acgagactca acgaggactc cggcccgagc gcgggcttgt tcccgccgac
     3181 cgctttcgaa tatttggcgc aggcgtacag actgtcgagc cgtccgatca gccgcgtgtt
     3241 ctccaggagc gcctcgcccc ggcgggcgat ctcggcggtg atggcccgca ggatgcggtc
     3301 gatctccagc cgctcctcct gctgcagcag gttgagccgg ttgttcagtt cgacggtctc
     3361 cggcggttcg atgtacagcg tggcccccgt ctgcgaacgg tcgtggagaa tccccatgtt
     3421 ggcccggtag gagctcgacg gcacggtgat gacgtagcgg ccgttgcgca tcgtcacgat
     3481 gtcgtcctgc acgccggcct ggtgggggcg gtcggcctga atggcggtga ggcgggcgac
     3541 gaggcgccgc ttggcgtcgc cgaattccgc ccgcacctgc ttgagcttgg gcgacgctga
     3601 gtccttcacc tcgccgttct cgtcaatggc gcgccggatc tccgccttca gctcggggaa
     3661 agcgcggacc tgctcgaggt aggcggcgat gagcgggaac ttcgggcgcc cctcccggtc
     3721 gtacccgttc agggcgatcg agacctcgac cagttcgagg acgcggagga tctgctcggg
     3781 atcgaggaat accccttcca cctgggcgcg gagaagttcg gggcggcagt tttccatgcg
     3841 ggcgaggggg aaggccatgc cgaagctgac gatatccctc atctgcgaga tttcgctgcc
     3901 gcggcgctcg atctcagccg ggtcgtacag aggccgtatg cggttgactt catccgcacc
     3961 atagggggtg aggcaccggc cggcgacctg ggcgatcacc ttcggatatt ccagtatgtc
     4021 aagagtatgc tggtcaatca tttcgcgttc tcgacggcgg cggca
//
LOCUS       DAOOKS010000075         4043 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_26778_length_4043_cov_2.945838, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000075 DAOOKS010000000
VERSION     DAOOKS010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4043
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26778_length_4043_cov_2.945838"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            392..751
                     /locus_tag="PK112_01945"
     CDS             392..751
                     /locus_tag="PK112_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010040634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HPC10842.1"
                     /translation="MNIYVGNLSFDTTEEQLRQAFSSHGEVTSVNIISDKYSGQSRGF
                     AFVEMPTKSEAIAAISALNGQEMNGRAISVNEARPREERGGGGGGRGGFGGGGRGGHG
                     GGRGGRSGGRGGDRGGW"
     gene            851..1255
                     /locus_tag="PK112_01950"
     CDS             851..1255
                     /locus_tag="PK112_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10843.1"
                     /translation="MTMAGQRKSFPCGHAGKGKYCHRCAAAEAERQKVAQIKGDWQSR
                     LRASPIPLDHLPKGVAENVLRVIEDLKSGKPYEDLKGKRLITMGQRDVISIPIQRRYR
                     LICRDREGKIEPIEAITHEVYNSRLSSGGWPK"
     gene            complement(1436..3355)
                     /locus_tag="PK112_01955"
     CDS             complement(1436..3355)
                     /locus_tag="PK112_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPC10844.1"
                     /translation="MRTIAFVAILLLLVGGCGDETTNNIYNTDTAAGGVFGRVSPPEA
                     GVLVSAWQSEQIASTATDAEGYFTLENLPPGLYEVRFAASDDREKVLGGIAIEGAGFI
                     NLGSIILASSWPIVSFDPADGDTVVPAATSLELRSEVELDLSSLDDAVVITPPVSDSW
                     SRGYDYSGRSYIYALEPTSGLLPDENYHVHITEQLALADGGPWGDTLSFSFRTAPMAL
                     TEINSSYDLADLSPGHSGWLCDLRFNAYVDESTLLTAVRIEPDVPFTASLSQSNLYTV
                     RIEVEGDFACGADYSIIVDTALHCLAGAGRATPDTIGFHTEPLRVVYRSYNYSPNALP
                     PRPQTLFEFDYNSDLDPETAEDAVSVDPPLELQVEVSSDYYSDTRELRVSILSQPTPG
                     ETYTVTIDSTLASSHGGTTEADEMVVLKIQPLTVTQIAVSNGSGFYETQTTLSPFGAF
                     YARIRFNAEVDENALNDAVTIDPPLPGFWYFAESYYDVGYYDFFPTQPVQLFPEQSFT
                     ITIDGDAPLIGSAALGEDKVFAFGVAPVAVAGVYPAPGTRTFYWYDDPYFRFNAPMER
                     TSTESAIRFTTMTGTPVNGVFQWSDGDKRVSFNPTASPVVGQAYVFHVDTSAASVAGP
                     HLKYADSTFFTVGEY"
     gene            3599..>4043
                     /locus_tag="PK112_01960"
     CDS             3599..>4043
                     /locus_tag="PK112_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020403688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HPC10845.1"
                     /translation="MKFDSLKDNFTIPAAEEKILRFWDENDVFHKAQKAHHDRPHFVF
                     YEGPPTANGRPGIHHVIARTVKDLVCRYKHMQGFRVDRKAGWDTHGLPVEIEVEKQLR
                     LETKSQVLEYGIGNFNAKCRESVFTFLDQWNEITRRIGYWIDLDDA"
BASE COUNT          880 a         1141 c         1319 g          703 t
ORIGIN      
        1 ggtagtggcc tgcgcggcaa gatggcatgc ggagggcggc ggtgtcaatg gttttccccg
       61 cacggcggtt tcgcccaggg cggattcccg ggggggcgag ccatgcggac agtgcgacaa
      121 cccgttggca aacaaacaat aagcgcggac cggcggcggg gaggcgaaaa atggacagga
      181 gaccgctaaa tttggtggac tttcggccga tcctagcgta tagtagttaa caatagcggt
      241 tgccgtattc gcttttcgaa gtgttctcaa taccgttgaa tctccgaaaa agctcgaccc
      301 cgggaccgat gcgcaatagt gcgcttgaat gtgttttctc cggtgatggc cgcaacggcc
      361 gccatcgggg cgaacgcgag tggagagtgg aatgaatatc tacgtcggca atctgtcgtt
      421 cgacacgaca gaggagcagt tgcgccaggc tttctctagc catggcgagg tcacgtccgt
      481 gaacatcatt agcgacaagt acagcgggca gtcccgcgga ttcgcttttg tggagatgcc
      541 cacgaagagc gaggcgatcg cggcgatcag cgctctgaac ggacaggaga tgaacggccg
      601 ggcgatcagc gtcaacgagg cgcgcccgcg cgaagagcgc gggggcggcg gcggcggacg
      661 cggcggattc ggcggcggcg gacgcggagg ccacggcggc ggacgcggag gccggagcgg
      721 cggacggggg ggcgatcggg gcggttggta aagccaccgg acaatgcgaa gcaagaaggg
      781 gccgagattc ggtccctttt tgcatagact ggggccgacg gggagcctca gaaccccgcg
      841 ggcgaggagg gtgaccatgg ccggacaacg aaagtctttc ccctgcggac atgccggtaa
      901 aggaaaatac tgccatcgct gtgcggcggc cgaggccgag cgacagaaag tcgcgcaaat
      961 caagggcgac tggcagtcgc ggctgcgcgc ctccccgatt ccgctggatc atctgccgaa
     1021 aggtgtcgcg gagaacgtcc tgcgggtaat agaggacctg aaaagcggaa aaccgtatga
     1081 ggacttgaag ggcaagcggt tgatcaccat gggccagcgc gacgtgatct cgataccgat
     1141 acaacgacga taccggctca tttgccgcga ccgggagggc aaaatcgagc ctatcgaagc
     1201 gataacgcac gaggtgtaca acagccggct gtcctcgggc ggctggccga agtgaggccg
     1261 acggcgaagt cgggatgttg cgcggagtac ggcccggggc gggagggcta ctacgctgct
     1321 cccgcgcgga ggatgccgga tgcggcgcag cgccagtgag aaggcaagag cccggaggct
     1381 cttgccatct cgctaccgcc cgggtcggga tccccaggga ctccctcgct gcgggctagt
     1441 actcgcccac cgtgaagaat gtggaatcgg cgtatttcag atgcggcccg gcgacgctgg
     1501 cggcgctcgt gtcgacatgg aagacgtagg cctgtcccac cacgggactt gccgtcggat
     1561 tgaaactcac ccgcttatca ccatccgacc actggaacac cccgttgacc ggggtcccgg
     1621 tcattgtggt gaagcggatg gcgctttccg tactcgtccg ctccattggc gcgttgaacc
     1681 ggaaatacgg atcatcgtac cagtagaaag tccgcgttcc cggtgcagga tagacgccgg
     1741 ctaccgctac cggagctaca ccaaacgcga acaccttgtc ctcgcccaag gcggcactgc
     1801 cgattaacgg cgcatccccg tcgatggtaa tggtaaagga ttgctccgga aagagctgta
     1861 ccggctgtgt cgggaagaag tcataatatc cgacgtcata gtagctttca gcgaaatacc
     1921 agaagccggg aaggggggga tctatcgtga cggcgtcgtt cagggcattt tcgtccactt
     1981 cggcattgaa ccgaatccgt gcgtagaaag ctccaaacgg gctgagcgtc gtttgtgtct
     2041 cgtaaaaccc cgatccgtta ctgacggcaa tttgcgtcac agtcagcggt tggatcttga
     2101 gaacgaccat ttcatcggct tccgtcgtcc cgccgtgaga agatgccagg gtagaatcga
     2161 tggtcacggt atatgtctcg cccggcgtcg gttgagagag tatgctcacc cgcagttccc
     2221 tagtgtcact gtagtaatcg gagcttacct ccacctgaag ctccagagga gggtcaacgg
     2281 agacggcgtc ctcggcggtc tcaggatcca ggtcggaatt gtaatcgaac tcgaacagcg
     2341 tctgcggcct gggcgggagc gcattgggag agtagttgta tgacctatac actacccgaa
     2401 gcggttccgt atggaacccg atggtgtccg gggtggcacg tccggcaccc gcaaggcaat
     2461 ggagtgcggt atcgacaatg atcgagtagt cagcgccgca cgcgaagtcg ccttcgacct
     2521 cgatgcgcac cgtgtagagg ttcgactggg agaggctggc cgtgaagggg acatcgggtt
     2581 cgattcttac ggcggtcagg agagtggact catctacata ggcgttgaac cgaagatcac
     2641 agagccatcc ggagtgaccg ggtgagagat ccgcgagatc ataggaggag ttgatttcgg
     2701 tgagagccat cggggcggtg cggaaggaga aactcagcgt atcaccccac ggcccgccgt
     2761 ccgccagcgc caactgctcc gttatgtgta catgatagtt ctcgtccggg agcaggccgg
     2821 acgtaggctc gagagcgtaa atgtacgatc tgcccgaata gtcatagcct cgcgaccacg
     2881 agtcgctgac gggtggcgtg atgaccacgg catcatcgag tgaggacagg tcaagttcga
     2941 cttccgatct cagctcgaga gaagtagccg ccgggacaac cgtgtcgcca tcggcgggat
     3001 cgaacgaaac gatcggccac gaactggcga gaatgatgct tccgaggttg atgaacccgg
     3061 ccccttcgat cgcgattccg cccagcacct tttcccgatc atccgaggcg gcaaagcgga
     3121 cttcgtacaa acccgggggc aggttctcaa gggtaaagta accttcggca tcggtcgccg
     3181 tcgacgcgat ttgctcggat tgccaggcgc tgacaaggac gccggcttcc ggcggggaga
     3241 cgcggccgaa aactccgccg gcggcagtgt cggtgttgta gatgttatta gtcgtttcgt
     3301 cgccgcagcc gccgacaagc agcaggagga tggccacaaa cgcgatggta cgcatacatg
     3361 actccttgcg gaaaagctcg gatttcgaaa ccctgcagtc cggccaaccg gggcggcagc
     3421 gacgccaccc ccggaggcca gcaccttcgg gatgtagtca acggcccggg aggagccggc
     3481 gcaagcactt tcgaccggaa cgcggagttg ccgaaaaacg cgcttgccgc aggggactgc
     3541 tttccgtata ctatcaagct atcggaaaac cgcttagccg gcgcgtgtgt aactgattat
     3601 gaaattcgac agtctcaaag ataacttcac gatcccggcc gccgaggaga agatcctccg
     3661 cttctgggac gagaacgacg ttttccacaa ggcccagaaa gcccaccacg accggcccca
     3721 cttcgtgttc tacgaggggc cgccgacagc caacggacgg ccggggatcc accacgttat
     3781 cgcccgcacc gtcaaagacc tggtctgccg ctacaaacac atgcaggggt tccgggtcga
     3841 ccgcaaggcg ggctgggaca cccacgggct cccggtcgaa atcgaagtcg aaaaacagct
     3901 ccggctggag accaaatccc aggtgctcga atacggcatc gggaatttca acgccaaatg
     3961 ccgggagtcg gtgttcacct ttctcgacca gtggaacgaa atcacacggc ggatcgggta
     4021 ctggatcgat ctcgacgacg cct
//
LOCUS       DAOOKS010000076         4040 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_26809_length_4040_cov_3.121455, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000076 DAOOKS010000000
VERSION     DAOOKS010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4040
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26809_length_4040_cov_3.121455"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="PK112_01965"
     CDS             complement(<1..406)
                     /locus_tag="PK112_01965"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HPC10846.1"
                     /translation="MTGRTFALCAAVVLLAAVRLPAAEIHDAVAAGDVERVRVLLAAD
                     PSLINARDSAQNLPLHVAALQGRTAMIELLLAEGADINIGDRENTSPLTIAAMRNHLD
                     AVRLLAERGAQAAARDINGETPLISAARRGNLE"
     gene            complement(693..1553)
                     /locus_tag="PK112_01970"
     CDS             complement(693..1553)
                     /locus_tag="PK112_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10847.1"
                     /translation="MQRVLIVLSALAVFLLLPLGCSDDESCADCPATPAGFVDGSITV
                     NPQAIVNAVIYGYGGIVPSIDSIRLGDSLMTRPTDYNWFQDAPGRRHWQIGFSESAGG
                     SFLYSSGDVARLEIWGPGRQAVCDVVMLDPRDSEAQIISPVPGEDTVAPGESVTIVWQ
                     RIEHAQFYALMLTFFNRANAVTRQSLVTDWTADTSYTVRPVLYPDSLQLISVELSGFT
                     GPNPSVGSNFSGGWLTGRLISFSAPAHTFIWGWNAPDKARAEFPDEAAVSAPRSPAEI
                     IDAIYRQAVR"
     gene            complement(1774..2631)
                     /locus_tag="PK112_01975"
     CDS             complement(1774..2631)
                     /locus_tag="PK112_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10848.1"
                     /translation="MQRIVLVLSAVAVSLLPLGCSDDCADCPEHATPAGFVNGTVHVT
                     PQVSLSATINGYGSILPTVDSIRLGDSLLTSSGDYYVAMSAPANPLWRISFYENISGD
                     VMYSPGDVAHIEIWGPGRLAYCDVVVLDPNESAADMISPLPGTETIAPDESAAVIWHR
                     ALDAQYYAVQLRFIDFANGVTRESNVTDWTADTAYTVGPTLYPDSLAALIVEITPFTG
                     PNPAVGSNFAGGWLAGRLISFGGPASTMLHGWNEPDKSIKTLPDDLAASQRLSPAEII
                     DGIYRQAAK"
     gene            complement(2848..3711)
                     /locus_tag="PK112_01980"
     CDS             complement(2848..3711)
                     /locus_tag="PK112_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10849.1"
                     /translation="MKRTMIVLSAVVVALLPLGCSDDDCAECPAPVTPVGFIQGSIDL
                     HPGVYFEALIFGDGSILPTLDSARVGDSLLVFNKDFFYSYRSEPVNPYWTIYCTEDTG
                     PDYMYTQGDTAHLEFWSEGRLATCDLPLLDPNLTQLFITDPYPGTDTVAPGEPAIVFW
                     NRVEHAQFYAVQVILANRVGDVFTFSDRFDWTADTSFTVSPSLYPPSLAQFSVRVVPF
                     TGPNPAAGSNVLGAWLSGRIVSVGYGAETAIVGWNPPPITKSALPAGETLFPQRTPAE
                     IVEAVYRQAAE"
BASE COUNT          817 a         1191 c         1360 g          672 t
ORIGIN      
        1 tctcgaggtt cccccgccgc gccgccgaga tcagcggggt ctcgccgttg atgtcccggg
       61 cggctgcctg ggcgccccgc tcggccaaga ggcggacggc gtccaggtgg ttgcgcatcg
      121 ccgcgatcgt cagcgggctc gtgttctcgc ggtcgccgat gttgatgtcc gctccctcgg
      181 cgagcaggag ttcgatcatg gcggtgcggc cctggagcgc cgcgacgtgg aggggcaaat
      241 tctgggcgct gtcgcgcgca ttgatcaggg acggatcggc ggccagcaga acgcgaacgc
      301 gctcgacatc gccggccgcg accgcatcgt ggatctcggc cgccggcagc cggactgccg
      361 ccagcagaac cacggcggcg caaagggcaa aggttcttcc tgtcatatct gtcctgccca
      421 tggatagggt cctcgggacg aggcgttttg tttaaatgtt atgacgcggg gcaaagcgga
      481 ttgttcagtc tatcaccgcg ccggcgtcgg cgtactttct gccccggcag gaaacagccc
      541 cgctctctgc attcaggcgg gagcgaacgt ctttaatgcg gactatttgt atacggattg
      601 gcacgccggc gccgccagga tggcacgacc ggacgggcgc cgccgggcga gccgcgcccg
      661 gcggcgaatc agtctggact caaggccggc cgctagcgga cagcctggcg gtaaatggca
      721 tcgatgatct cggccggcga ccgcggcgcc gacacagcgg cctcatcggg aaactccgcc
      781 ctcgccttgt ccggtgcgtt ccagccccag atgaaggtgt gtgcaggcgc gctgaaactg
      841 atcaggcgtc cggtcagcca gccgccggag aagttgctcc ccaccgatgg attcgggccg
      901 gtaaaaccgg aaagctcgac cgaaatgagc tggagggaat ccggataaag gaccggtcgc
      961 accgtgtagg acgtgtcggc tgtccagtcc gtgaccaggg actggcgggt cacggcgttg
     1021 gcgcgattga agaacgtaag catgagggcg taaaactggg cgtgctcgat ccgctgccag
     1081 acgatggtca ccgattcgcc gggggccacc gtgtcctcgc cgggaacagg gctgatgatc
     1141 tgcgcttcag aatcgcgggg atcgagcatg accacatcgc acaccgcctg gcggccgggt
     1201 ccccaaatct caaggcgggc gacgtcgcct gaagaataca ggaaggagcc gccggccgat
     1261 tcgctgaacc ctatctgcca gtggcgacgg ccgggggcgt cctggaacca gttgtagtcc
     1321 gtgggccgcg tcatgagcga atcgccgagg cggatggaat cgatgctcgg aacaataccc
     1381 ccataaccgt agataacggc gttcacgata gcctgggggt tgactgttat gctgccgtca
     1441 acaaaacccg ccggcgtcgc cgggcagtcg gcgcagctct cgtcgtcgct gcagccgagc
     1501 ggcagcagca ggaagacagc cagggctgaa agaacgatca aaacgcgctg catgagaagc
     1561 ctccttgtga tgagcccggc tcgcggcatt tcccacggcg tgcgaccggg ccgcaagtta
     1621 cgctcaagaa cgaaacatgg cgcggagggg gacagcaagg aaaatcggcg gcgagttctt
     1681 ctgtaatcaa tcggtggcca gcccggccaa cagcaggccg ccgggccgaa acccggcggc
     1741 ggtgcgcgtg gtgcgaggac gagaagcagc agtctacttt gcggcctggc gatagattcc
     1801 gtcaatgatc tcggcggggg agagccgttg cgaagcggct aggtcatccg gtagcgtctt
     1861 gatcgacttg tccggctcat tccagccatg tagcattgtg ctcgcggggc cgccgaaact
     1921 gatgagtcgc cccgccagcc acccgccggc gaagttgctt ccgacagccg ggttcgggcc
     1981 tgtaaagggt gtgatttcaa ctatgagagc cgccagagaa tcggggtaca gggttggacc
     2041 aacagtatag gcagtgtcgg cggtccagtc ggtgacattc gattcgcggg tcacgccgtt
     2101 ggcgaaatcg atgaatctga gttgtaccgc gtagtactgg gcgtccagcg ctcgatgcca
     2161 gatgacggcg gccgactcat ccggggcaat ggtctccgtt ccgggaagag ggctgatcat
     2221 atcggcagcg gactcattgg gatcgagcac gaccacatcg cagtaagcca agcggccggg
     2281 tccccagatc tcgatgtgcg ccacgtcgcc gggcgaatac atgacgtcgc cgctgatgtt
     2341 ctcgtagaag gatatgcgcc agagaggatt cgcgggagcg ctcatcgcaa catagtaatc
     2401 gccggacgac gtcaggagcg aatcacccag ccggatggag tcgaccgttg ggaggatgct
     2461 cccgtaaccg ttgatcgtgg ccgacaacga cacctgcgga gtcacgtgaa ctgtgccgtt
     2521 aacgaagcct gcgggagtcg cgtgctcggg acagtccgcg cagtcatcgc tgcagccgag
     2581 cggcagcagg ctgaccgcca cagcggagag aacaagaacg atgcgctgca tgagaaatct
     2641 cctggtgatg agttgtatct acaccggttt cggagaaacc gaaccgaacg gcaaaccaca
     2701 tgatagaacg agaccgcctg cccctccggc gagcaagata aaacggcggc cattccgtct
     2761 ctgaccggtt tcaccgcacg gatttgccac ttcaccgccg gatgggagta aacaggcggc
     2821 ggtgaatttg ccgcgagaga caggcagtta ctcggcagcc tgccggtaga ctgcctcgac
     2881 aatctcggcg ggcgtccgct gcgggaagag cgtctccccg gccgggagtg ccgacttcgt
     2941 aatcggggga ggattccacc ctacgatggc cgtctcagcc ccgtacccta cactgacaat
     3001 ccgtccggac aaccacgccc cgagtacgtt gctgccggcg gcggggttgg ggccggtgaa
     3061 cgggaccacc cgaaccgaga actgagcgag tgagggagga tacagactgg gcgacacagt
     3121 gaacgaggtg tcggcggtcc agtcgaaccg gtcggagaac gtgaaaacat cgcccacgcg
     3181 gttggcaagg atgacctgca cggcatagaa ctgggcatgc tccacgcggt tccagaacac
     3241 gatggcgggt tcacccgggg cgacggtatc ggtcccgggg tagggatcgg tgatgaataa
     3301 ctgcgtcaga ttggggtcga ggagcgggag atcacaagtg gcgagacgcc cctcactcca
     3361 gaattcgagg tgggccgtat cgccttgggt atacatgtag tccgggccgg tgtcttcggt
     3421 gcagtagatc gtccagtagg ggttgaccgg ctcgctccga tacgagtaga agaaatcctt
     3481 gttaaaaacc agtagcgaat cgccgacgcg cgctgagtcg agcgtgggca ggatactccc
     3541 atcgccgaaa atcaaggctt caaagtaaac gcccgggtgc aggtcaatgc tgccttgaat
     3601 gaacccgacc ggcgtcacgg gcgccggaca ctccgcgcag tcgtcatcgc tgcacccgag
     3661 gggcaacagt gcgaccacca cggcggaaag gacgatcatg gtccgcttca tgagttacct
     3721 cctgtgaagg atatatggcc gagatggttt ccgtgaaaca gtaccgttca acggaatctc
     3781 ctgcggaaaa atgaattcgc gccacgagtt gcggcaacac aaaaacgcgg ccgggcacaa
     3841 cggcgcagcg ggttgtcgcc gcagcgccgc ggttaccggg gtgcagagag cgtttcagtc
     3901 tgcgcctgat cgccggtcgc cgatggcatc cggcggcaat agtgctcgta gcgggcgaag
     3961 acgtcccgga cgaagtcggc cgtctcggcg ctgcgaaggt agccgaagcg gagggtctca
     4021 tcctcgaccg cgggggagtc
//
LOCUS       DAOOKS010000077         4017 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_27027_length_4017_cov_3.159263, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000077 DAOOKS010000000
VERSION     DAOOKS010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4017
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27027_length_4017_cov_3.159263"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="PK112_01985"
     CDS             <1..521
                     /locus_tag="PK112_01985"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HPC10850.1"
                     /translation="TAEFLGVDIGNVEEMLVKANQEIWKSYLTIENLFKERQALTQKL
                     LAEERAKGAIESKNIAMATLSHYLNNAVMAIYGRSQLLRMQHGKGAAASPLLEKLPSN
                     LDVIDRSVKKIVAVLEEMKELSPIDKQKFYNMSEALNIDDRIARRMESMETSGDIGGV
                     LTAAEEAAHLGS"
     gene            553..1752
                     /gene="aroC"
                     /locus_tag="PK112_01990"
     CDS             553..1752
                     /gene="aroC"
                     /locus_tag="PK112_01990"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HPC10851.1"
                     /translation="MLTYVTAGESHGPQLTAILDGLPAGFRIDPAEVNYQLARRQKGF
                     GRGGRMKIERDEAEFVSGVRGGVTMGGPITLVIRNRDWENWARIMHPLEPMPGELDLR
                     DRQRTAATRRPRPGHADLPGAVKWNHHDMRNVLERASARETAARTALGALARQLLEHF
                     GVEFASHVVRIGEVEVPRGYDLSDLTRVREVTEASEVRCLDAAAGERMIEAIRAARAV
                     RDSLGGIVEIIVRGLPIGLGGFSQWRQRLDGRLAGALMAVPSVKGVEIGLGFAAAGRR
                     GSEAHDEIFYDPDGDPRRKRFLRRTNNAGGIEGGVTNGEDVVVRVAGKPLSTLNRPLR
                     TVDVATKEPGEAMVERTDTCAVPALGVIGEGVAALVLAEAFLEKFGADNLAETERNFT
                     TYLDTQF"
     gene            1862..2998
                     /locus_tag="PK112_01995"
     CDS             1862..2998
                     /locus_tag="PK112_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10852.1"
                     /translation="MRRMLVPAAAAVLTAALIVGCGDGDEPTGPAPDQWYTEAVDTAG
                     RTGLFTSIAVDGSDRPHLVYYNEFGPDNLKYAHYDGAQWQFAVIDANTATSDLAPPVI
                     LVDRAGGSHVAFIDQTADEVRYASELVNGWQVETVDGAGERFCALSMTSTADGVPVIS
                     YGKTISDSVAGLWLAVLDVQADAWEIAAIDSGAAIVASSAAIDSQGLCHVAYIGNAGY
                     PGFDLYYAVGSPGAWAIDTVVGDTTAWYQCSLGVTPDGEPQIAFNSVDPAGMAYAVKT
                     ETGWSIEVIDGAALGPAPEMRTPDGEQLSLRLDAGGVPHLAYWSWADSSLVYAVRGTG
                     WAAERADNSGSVGAYCSLALDHQGRPHIAYQDRDRADLKYAIKR"
     gene            3040..>4017
                     /locus_tag="PK112_02000"
     CDS             3040..>4017
                     /locus_tag="PK112_02000"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HPC10853.1"
                     /translation="MILKYSAEDPAGTAADCLVLCVTQYDKPVDPYLKRLDAGTRGGI
                     TRLRQTEEFFGKDGQYAVILSPEGFAAPQVVLVGVGERRVAGHDAYRRALGRIANLPA
                     LTRAESLAIHLGTATRADCFQAAAEGYLLGGWKQREFKSGEEAKDPRRTTRITFMVKG
                     RAQLRPLQQAVERGWTIAEGQLLVRRLAGTPSNRLTPSLYAQEAAALARRHKIEVTVL
                     DEAAIKKEKMGALLAVAQGSAQKPRFVVLRYHGGGRGQKPVVLVGKGVTFDTGGISLK
                     DPLNMHEMKGDMAGSAAVLAALVTAARLRLRINLVGLMPLAENMPSGTAT"
BASE COUNT          669 a         1215 c         1526 g          607 t
ORIGIN      
        1 cgacggcgga atttctcggc gtcgatatcg ggaacgtgga ggagatgctg gtcaaggcga
       61 accaggaaat ctggaagtcg tacctgacga tcgagaacct cttcaaggag cggcaggcgc
      121 tgacccagaa gctcctggcg gaggagcggg cgaagggggc gatcgagtcg aagaatatcg
      181 ccatggcgac tctgtcgcac tacctcaaca acgcggtgat ggcgatctac gggcggtcgc
      241 aactgctccg gatgcagcac ggcaagggag cggcggcgtc gccgctgctg gagaagctcc
      301 cgtccaatct cgacgtgatc gaccggtcgg tgaagaagat cgtggccgtg ctcgaagaga
      361 tgaaagagct ctcgcccatc gacaagcaga aattctacaa catgtcggag gcgctgaata
      421 tcgacgaccg gatcgcgcgg cggatggagt cgatggagac ctcgggggat atcggcggcg
      481 tgctcacggc ggccgaagag gccgcccatc tcggcagttg accggcaggc ggcggcgccg
      541 taccatgggg tcatgctgac gtatgtgaca gccggcgaat cgcacggccc gcaactgacg
      601 gcgatcctcg acggtcttcc ggcggggttc cggatcgacc cggccgaggt caattaccag
      661 ttggcgcggc gccagaaggg attcgggcgg ggcgggcgca tgaaaatcga gcgcgacgag
      721 gccgagttcg tctcgggtgt gcgcggcggg gtcaccatgg gcggaccgat cacgctggtg
      781 atccggaacc gcgactggga aaactgggcg cggatcatgc acccgctcga accgatgccg
      841 ggggagctgg atctgcgcga ccggcagcgg accgccgcca cccggcgtcc ccggcccggg
      901 cacgccgacc ttccgggggc cgtcaaatgg aaccaccacg acatgcgcaa cgtgctggag
      961 cgggcttcgg cgcgggagac ggcggcgcgg acggcgctcg gggcgctggc gcgccagctg
     1021 ctcgagcatt tcggcgtcga gttcgcttcc cacgtcgtgc ggatcggcga ggtcgaagtc
     1081 ccccgcggct atgacctcag cgatctgacc cgggtccgcg aggtcacgga ggcctcggaa
     1141 gtgcgctgtc tcgacgccgc ggccggagag cggatgatcg aggcgatccg ggcggcccgc
     1201 gcggtgcgtg attcgctggg gggaattgtc gagatcatcg tccgcgggct gccgatcggc
     1261 ctgggcgggt tctcgcagtg gcggcagcgg ctcgacggcc gcctggcggg ggcgctcatg
     1321 gcggtgccct cggtgaaggg ggtcgagatc ggcctgggtt tcgccgccgc agggcggcgc
     1381 gggtccgaag cgcacgacga aatcttctac gatcccgacg gcgacccgcg gcgcaagcgg
     1441 ttcctccgcc ggacgaacaa cgcgggggga atcgaggggg gcgtcaccaa cggcgaggat
     1501 gtggtcgtgc gggtggcggg aaaaccgctc tccacgctca atcggccgct ccggacggtg
     1561 gatgtggcca cgaaggagcc gggggaggcg atggtggagc ggaccgacac ctgcgccgtg
     1621 ccggcgctgg gcgtgatcgg cgagggcgtg gcggcgctcg tgctggcgga ggcgttcctg
     1681 gagaagttcg gggcggacaa cctggccgaa acggaacgca acttcaccac ctatctcgac
     1741 acgcaattct agccccttat gtggccctcc ggccggtgtg catcgggctg tcgcggctgt
     1801 tgccccggcc gcgggaatcc gctatactgc ccctgaatct ggctgacgga aaggggtagt
     1861 catgagacga atgcttgtgc cggcggccgc ggcggtgttg acggcggctc tcattgtcgg
     1921 ctgcggcgac ggagacgagc cgacgggtcc cgcgccggat cagtggtaca ccgaggcggt
     1981 cgacacagcc gggcggaccg ggctgttcac ctcgattgcc gtggacggat cggatcggcc
     2041 ccacctggtc tactacaacg agtttgggcc ggacaatttg aagtatgcgc actatgatgg
     2101 ggcgcaatgg caattcgcag tcatcgatgc caacaccgcg acctcggacc tcgcgccgcc
     2161 ggtgattctg gtcgaccggg ccggcgggtc gcacgtcgct ttcatcgacc agactgccga
     2221 cgaggtgcgc tacgcctccg agctggttaa cggctggcag gtggagacgg tcgacggggc
     2281 gggggaacgt ttctgcgcgc tgtcgatgac gtctacggcc gacggcgtcc cggttatctc
     2341 ctacggcaag acaatcagcg attcggtcgc gggcctgtgg ctggcggttc tcgacgtgca
     2401 ggcggacgcc tgggaaatcg cggcgatcga ttcgggggcc gcgattgtcg cctcgtcggc
     2461 ggccatcgat tcgcaggggc tttgccacgt cgcctatatc ggaaacgccg gatacccggg
     2521 attcgatctg tactacgcgg tcggctcgcc gggagcctgg gcgatcgaca cggtggtggg
     2581 cgacaccacg gcctggtacc agtgctcgct gggcgtgacg ccggacgggg agccgcagat
     2641 tgcgttcaac tcggtcgacc cggcggggat ggcgtatgcg gtgaagaccg agaccgggtg
     2701 gtcgatcgaa gtgatcgacg gggcggcgct gggtccggcc cccgagatgc gcacgcccga
     2761 cggcgagcaa ttgagtctgc ggctcgacgc cggcggggtg ccgcacctcg cctactggag
     2821 ctgggccgat tcctcgctgg tgtacgccgt gcgcgggacc ggctgggcgg cggagcgggc
     2881 ggacaattcg ggatcggtgg gcgcctactg cagtctggcg ctcgaccacc agggacgccc
     2941 ccatattgcc taccaggacc gcgaccgcgc ggacctgaaa tacgcgatca agcgctaacg
     3001 gcgcactcaa cggacggcgt gcgataaatg gagtctcaca tgatactgaa gtattcggcg
     3061 gaagacccgg ccgggaccgc agccgactgc ctggtcctgt gcgtgacgca gtatgacaag
     3121 ccggtcgatc cgtatttgaa gcggctcgac gcggggacgc ggggggggat cacgcggctg
     3181 cgccagacgg aggagttttt cggcaaggac gggcagtatg cggtgattct gagcccggag
     3241 ggtttcgccg ccccgcaggt ggtgctggtc ggggtcggcg agcgccgtgt cgccggccat
     3301 gacgcctacc gcagggcgct ggggcggatc gcgaacctgc cggcgctcac ccgcgccgag
     3361 tcgctggcga tccatctcgg cacggcgacg agggcggatt gctttcaggc ggcggcggaa
     3421 gggtatctgc tcggcgggtg gaagcagcgc gagttcaagt ccggcgagga ggccaaagac
     3481 ccgcggcgga cgacccggat cacgttcatg gtcaaggggc gggcgcagct caggccgctg
     3541 cagcaggcgg tggagcgcgg gtggacgatc gcggagggac aactcctggt gcggcgcctg
     3601 gccggcacgc cgtcgaaccg cctcacgccc tcgctctacg cccaggaggc cgcggcgctc
     3661 gcccgccggc acaagatcga ggtcacggtg ctcgacgagg ctgcgatcaa aaaggagaag
     3721 atgggagcgc tgctcgcggt cgcgcaggga tcggcgcaga aaccgaggtt tgtcgtgctg
     3781 cgctaccacg ggggcgggcg cgggcagaag ccggtggtgc tggtcggcaa gggcgtgaca
     3841 ttcgacacgg gcgggatcag cctgaaggat ccgctcaaca tgcacgagat gaagggggac
     3901 atggccggct cggcggcggt cctggcggcg ctcgtgacgg cggcgcgcct gcggctccgg
     3961 atcaacctgg tcgggctcat gccgctggcg gagaatatgc cctcgggtac ggccacg
//
LOCUS       DAOOKS010000078         4016 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_27038_length_4016_cov_3.302701, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000078 DAOOKS010000000
VERSION     DAOOKS010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4016
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27038_length_4016_cov_3.302701"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1279
                     /locus_tag="PK112_02005"
     CDS             <1..1279
                     /locus_tag="PK112_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HPC10854.1"
                     /translation="IDDVTLDWLRRRIEFVRRRGLAGVPSDADGGGEHPRLRTARLED
                     LQRHIPAADPGAFYARELMLDLATVTPHVSGPNHVKVMTSVADLARQKKRIHKAYLVS
                     CVNSRVEDLAAAAAVVRGKRVAAGVEFYIAAASSEVQAESEKRGDWAALLAAGAIPLP
                     AGCGPCIGLGRGLLGEGEVGISATNRNFKGRMGAREAEAYLASPAVVAASAIAGYITG
                     PAEATARPTTGEMRVNRKPAGVTKAARVIDGFPTRLSGRLAFCRQDNLNTDGIYPGKY
                     TYIDDMTPEQMAAVVMENYDPEFGKLVRKGDILVGGFNFGTGSSREQAATALQHRGIA
                     LVVAGSFSETYKRNALNNGFLAIECPDLVNDLKAACDPNRLTAVSDSEATVDFAAASL
                     VFAGKTYEVDPIGAAAQELVLSGGLEGWVRQRL"
     gene            1332..2093
                     /locus_tag="PK112_02010"
     CDS             1332..2093
                     /locus_tag="PK112_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10855.1"
                     /translation="MALKYHSSVASALAALIAAGPLFLACGSDDGNPFGGGGPDHALN
                     PAEYFGLVPGRTITAGYVNYVGDSLRQFVPNSPVDAWSFTPVIGLPTICNGRLAYPVS
                     AAPIPSNYSDPDYAFTLYFSADSSGLYWHGGSYCGLDLPLPSPGRIAPYPLASGLKWQ
                     TIGHAETDIDQCNRSVADTLNATCRRITNAVVRGNAYPEVFLIRIPFIDVPGHIGTRY
                     YEAWIAPGYGPVRGASFDQAYEHPDEGFEFISYSP"
     gene            2171..3382
                     /locus_tag="PK112_02015"
     CDS             2171..3382
                     /locus_tag="PK112_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009108527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HPC10856.1"
                     /translation="MDWLIILVFGVVYLGMILGRIPGLALDRTGVALLGALCLLGFGL
                     VTTADLPAAVDMSTIALLFALMVVSSQFRLAGAYSFLVRRAGELAVSPAALLAVVIGV
                     TALLSAVLVNDIVCLAATPVVIEACAHRRLNPVPYLLGLAAAANIGSAATLIGNPQNI
                     LIGEVLRLSFSGYLLESLIPVVFGLVLLWLILAWQYRGRLGAETVAPPVAAPLFSRWQ
                     TAKGTLVLAALLVIFLAGSWPRDLAALGAAGVLLLSRRMQSREMLGLIDWPLLVLFLA
                     LFVVNYAVNSGGYMAGVMVGLDAAGIPLDHPGWLFAASVVLSNLVSNVPATMLLLPSA
                     THPLGGAVLALSSTLAGNLLVVGSIANIIVLDQAERLGVRISWRRHARTGVPVTAATL
                     LAAGVWLWLRA"
     gene            complement(3387..4016)
                     /gene="amrA"
                     /locus_tag="PK112_02020"
     CDS             complement(3387..4016)
                     /gene="amrA"
                     /locus_tag="PK112_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HPC10857.1"
                     /translation="MVGYLSAVFLSERKAAAREVIGARAAGEPRKREERIADDDRRLL
                     AAVARDAIRARLEGREYAPPAPESLAMQKGAFVTLHLQGQLRGCIGRLRSDEPLPRTI
                     ADMAVAAAFEDPRFPELHERELDRIDIEISILSPLARVRDIEEIQVGRDGLMIKLDWH
                     SGLLLPQVAAENGWDRMEFLGQTCLKAGLPKSSWKDKNAEIYRFSAEVF"
BASE COUNT          578 a         1312 c         1436 g          690 t
ORIGIN      
        1 catcgacgac gtcaccctgg actggctccg ccggcggatc gagtttgtgc ggcggcgcgg
       61 gctcgccggc gtgccctcgg acgccgacgg cggcggggag cacccccggc tccgcacggc
      121 gcggctggag gacctccaac gccacatccc ggccgccgat ccgggggcgt tctatgccag
      181 ggagctcatg ctcgacctcg ccacggtcac cccgcacgtc tccgggccga accatgtcaa
      241 ggtcatgacc tcggtggccg acctggcgcg tcagaagaag cgcatccaca aggcctatct
      301 cgtgtcctgc gtgaacagcc gggtggagga tctcgcggcc gcggctgcgg tggtgcgcgg
      361 aaagcgggtg gccgcagggg tcgagttcta catcgccgca gcctcgtccg aggtgcaggc
      421 ggagagcgag aagcggggtg actgggcggc tctgctcgcg gccggggcga tcccgctccc
      481 ggccggctgc ggaccgtgta tcggtctggg ccgggggctg ttgggcgagg gcgaagtcgg
      541 gatctccgcg accaaccgga atttcaaagg ccgcatgggc gcgcgcgagg cggaggccta
      601 cctggcctcg ccggcggtgg tggccgccag cgcaatcgcc ggctacatca ccggaccggc
      661 cgaggcgacg gcacggccga ccaccggcga gatgcgggtg aaccgaaaac ccgccggagt
      721 aaccaaggcg gcgcgggtga tcgacggctt ccccacccgg ctgtccgggc ggctggcttt
      781 ctgccgccag gacaacctca acaccgacgg gatctacccc ggcaagtaca cctatatcga
      841 cgacatgacg cccgagcaga tggcggcggt ggtgatggag aactacgatc ccgaattcgg
      901 caagctcgtg cgcaaggggg atattctggt cggcgggttc aatttcggca ccggctcctc
      961 ccgggaacag gcggcgacgg cgctgcagca ccgcgggatc gcgctggtgg tcgccggctc
     1021 gttctccgag acctacaagc ggaacgccct gaacaacggt tttctcgcga tcgaatgccc
     1081 cgacctggtg aacgatctga aagccgcgtg cgacccgaac cggctgacgg cggtttccga
     1141 cagcgaggcg acggtcgatt ttgcggccgc gtcgctggtg ttcgcaggca agacctacga
     1201 ggtcgatccg atcggcgcgg cggcgcagga gctggtgctc agcggcgggc tggaaggatg
     1261 ggtgcggcag cggctgtagg agagaacggc cgagagacgg cattggccaa cccccacgcg
     1321 gaggaataga catggcgctc aagtatcaca gctcagtcgc gtcggcgctg gccgcgctta
     1381 ttgccgccgg ccccctgttc ctcgcgtgcg gttccgatga cggcaacccc ttcggcggcg
     1441 gcgggccgga ccatgccctc aacccggccg agtatttcgg cctggttccg ggccgaacca
     1501 taacagccgg gtacgtgaac tatgtcggcg acagtctccg ccagtttgtg ccgaacagcc
     1561 cggtcgacgc ctggtccttc accccggtca taggtctgcc gacgatctgc aacggtcggc
     1621 tggcctaccc ggtgtccgcc gcgcccatcc cgagtaacta ttccgacccg gattacgctt
     1681 tcacgctcta cttcagcgcc gactccagcg gcctgtactg gcacggcggc tcgtactgcg
     1741 gactggacct tcccctgccc tcgcccggca ggatcgcccc atacccgctg gcgagcggat
     1801 tgaagtggca gacaatcggc catgcggaga ccgacatcga ccagtgcaac cggtcggtcg
     1861 ccgatacgct caacgcaacc tgtcgacgga ttacgaacgc cgtcgtgcgc gggaatgcct
     1921 acccggaggt attcctgatc cgaataccgt tcatcgacgt gccggggcat atcgggacgc
     1981 gctactacga ggcgtggatc gccccgggct acggtccggt gcgcggcgcg agctttgatc
     2041 aggcgtacga gcatccggac gaggggttcg agttcatttc ctactccccg tgaggctccc
     2101 cggggccgat tccccggcgc cgggacgata gctttcagcc ggcgcgggca gcggcccgaa
     2161 aggcaaggcg atggactggc tcatcatact cgtgttcggc gtcgtgtacc tcgggatgat
     2221 ccttgggcgg attcccggtc tggcccttga ccggaccggg gtggcgcttt tgggggcgct
     2281 ctgtctgctg gggttcgggc tggtgacgac ggccgatctg ccggcggccg tggacatgtc
     2341 gacgatcgcg ctgctgtttg cgctcatggt ggtgtcatcg cagtttcggc tggccggggc
     2401 ctactccttc ctggtgcggc gagcggggga gctggcggtg tcgccggcgg cgctgctggc
     2461 ggtggtgatc ggggtgacgg cgctgctgtc ggccgtgctg gtcaacgaca ttgtctgtct
     2521 ggcggcgacg ccggtggtga tcgaggcctg cgcccaccgc cgcctgaacc cggtcccgta
     2581 cctgctgggg ctggcggcgg cagcgaacat cggctcggcc gcgaccctga tcggcaaccc
     2641 gcagaacatc ctcatcgggg aggtcctccg cctgtcgttt tcggggtacc tgctcgagag
     2701 cctgattccg gtggtgttcg ggctcgtatt gctgtggctc attctcgcct ggcagtaccg
     2761 gggccggctg ggagcggaga cggtggcgcc gccggtcgcg gccccgctgt tcagccggtg
     2821 gcagacggcc aaggggacgc tcgtgctggc ggcgctgctg gtgattttcc tggccggcag
     2881 ttggccgcgc gacctggcgg cgctcggcgc ggcgggagtg ctcctgctgt cgcggcggat
     2941 gcagtcgcgc gagatgctcg ggctgatcga ctggccgctc ctggtgctgt ttctcgcgct
     3001 cttcgtggtc aactacgcgg tcaacagcgg cggctacatg gccggcgtca tggtcggact
     3061 ggacgcggcc ggcattcccc tggaccaccc ggggtggctc ttcgcggcga gcgtcgtgct
     3121 gagcaacctg gtgagcaatg tgcccgcaac catgctcctg ttgccctcgg cgacccatcc
     3181 cctgggcgga gcggtgctcg cgctgtcatc gacgctggcg gggaatttgc tcgtggtcgg
     3241 ctcgatcgcg aacatcatcg tgctcgacca ggcggagcgg ttgggagtgc gcatttcatg
     3301 gcggaggcac gcccggacgg gagtgccggt gacggcggcg acgctgctgg cggcgggggt
     3361 atggctgtgg ctgcgggcgt agtctctcag aacacctcgg cgctgaaacg atagatctcg
     3421 gcgttcttgt ctttccagct gcttttcggc agcccggctt tcaggcacgt ttgcccgagg
     3481 aattccatcc ggtcccagcc gttttccgcg gcgacctgcg ggaggagcag cccggagtgc
     3541 cagtcgagtt tgatcatgag gccgtcgcgg ccgacctgga tctcttcgat gtcgcgcacc
     3601 cgcgcgagcg gcgagaggat cgagatctcg atatcgatac ggtcaagctc gcgttcgtgc
     3661 agttcgggga accgggggtc ctcgaacgcc gccgccaccg ccatgtcggc aatcgtccgg
     3721 ggcagcggct catcggagcg gagccgcccg atacagccgc ggagctgtcc ctggaggtgg
     3781 agggtgacga acgcgccttt ctgcatggcc agcgactcgg gagcgggcgg ggcgtactct
     3841 cggccctcca ggcgcgcgcg gatcgcgtcg cgggcgaccg ccgccagcag ccgccggtcg
     3901 tcatcggcga tgcgctcctc cctttttcgc ggttcgccgg cggcccgggc gccgatgacc
     3961 tcgcgggcgg ccgctttccg ctcggacaga aagacggccg agaggtagcc aaccac
//
LOCUS       DAOOKS010000079         4008 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_27106_length_4008_cov_2.693903, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000079 DAOOKS010000000
VERSION     DAOOKS010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4008
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27106_length_4008_cov_2.693903"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="PK112_02025"
     CDS             <1..521
                     /locus_tag="PK112_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018490386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HPC10858.1"
                     /translation="AEFGPRALGFRSILADPRDPRMKEKINAAVKFREPFRPFAPAVM
                     CEHAADYFECDRPAPHMLVNFTVRADKRSLIPAVTHVDNSTRIQTVAEADNPPLYRLL
                     AEFKSLTGIPVLLNTSFNLRGHPIVNTPEEAFATFCSGGLDILLLGDYLVKKEEIPEA
                     LRQRFRLVQAED"
     gene            567..728
                     /locus_tag="PK112_02030"
     CDS             567..728
                     /locus_tag="PK112_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009543764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HPC10859.1"
                     /translation="MAGRVKILAEFWYFLRTSRKWWLGPILVLLVLVSALILFTESSA
                     LAPFIYSLF"
     gene            787..1827
                     /locus_tag="PK112_02035"
     CDS             787..1827
                     /locus_tag="PK112_02035"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HPC10860.1"
                     /translation="MPPRDTYPVLFRTAAVLLSLALFVALAEGVVRLAGLDPYFQNRF
                     FTVNRALDYPEIFDKDHELFWRFRPDQSVTSRFFIGRTHRINSAGLHGPEITPAEGRT
                     RVITLGNSCTFGWGVATEHGYPRLLDSLLGDSFQVINAGIPGYTSLQGKRFYRSDLSR
                     LRPAIVTILFAFNDHWAAAGDRADKDQRMPPQWLLDIQNALGRLHTYRLLKKAILSAV
                     EPPMDSLFDRRTPIYRVGLEDFRANLEDLCRMIVADSARPILLTSPIPDLPTYYPKGY
                     QSNLHKYHERYNAAIREMAAARGYDVADLAEGFAGRPGLFDDPLVDPAHFNDFGHRLA
                     AAMIAACIRDSL"
     gene            complement(1828..2997)
                     /locus_tag="PK112_02040"
     CDS             complement(1828..2997)
                     /locus_tag="PK112_02040"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPC10861.1"
                     /translation="MTTSTAEQFRAVRGEFPHTRELVYFNSASYGPYSTRVQRALAET
                     IAARVRSAEDSTASIFAMREALRRDYGALIGARPSQIGIGLNTTFGLNVAAYGLPLKK
                     GDEVLLTDVEFPALVYAFRGAAQTRGITLRFVKTRDRRVDVEQLSRAVTKRSKVLAIS
                     WVQFFDGYRNDLAALGEFCRDHGLFFVVDGIQGTGMQPLDVRKLGIDIFSTGCQKWLL
                     APQGSGFFYIADRIRDRLQPPSASWLGVEWHQKFGDLFRYDREFLATAARFELGYYVE
                     LNLAGMRESAAMFRELGTRNIQRHTRALIDRLADYIRGHKFYRITSSMEPKHRSSIFT
                     FTCDRFRDLHKELYRRKITCVHREGSIRISVHFFNNEDDIDKLIAVLDRFAESAP"
     gene            complement(3005..3433)
                     /locus_tag="PK112_02045"
     CDS             complement(3005..3433)
                     /locus_tag="PK112_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10862.1"
                     /translation="MAHVINMTLMAYCLIAGGVYLLVVTLAHEWLGRKLDMAKGIPPA
                     QREAWGPAAFVVGYVMEGLFFVAIPTLAYSFFTAVLPLAGIRTGLALALTGFVLGGVP
                     AIMGLSLRVRLWMPHLLYFLLGLLLKLGGCLAIIGYLYSL"
     gene            complement(3421..>4008)
                     /locus_tag="PK112_02050"
     CDS             complement(3421..>4008)
                     /locus_tag="PK112_02050"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPC10863.1"
                     /translation="PQPITTYGRSKLAGERAVLSFADRFHVISLPPAAVYGPGDRELL
                     TFFAAAHRGLRPVIGDPRRKLQLVHADDLSRAVVMALTGGTRAGEAYFIAEEEAYAMG
                     RLVDLVAAAGGRKGIGLRLPGGVFRAIAAVSEGLFRLAGATPMLTREKCRELLASWEV
                     STAKARAEFGFQAQIGFADGARETFAWYRREGWLT"
BASE COUNT          702 a         1462 c         1142 g          702 t
ORIGIN      
        1 gcgccgagtt cggaccgcgc gccctcggct tccgttctat cctggccgat ccccgcgacc
       61 cccgcatgaa ggagaagatc aacgcggccg tgaaattccg cgaaccgttc cgccccttcg
      121 ccccggcggt catgtgcgag cacgccgccg actacttcga atgcgaccgc cctgcccccc
      181 acatgctcgt caacttcacc gtccgcgccg acaagcgcag cctgattccc gcggtcaccc
      241 atgtcgacaa ctccacccgc atccagacgg tggccgaggc cgacaacccg cccctgtacc
      301 gcctcctggc cgaattcaaa tccctcacgg gaattccggt cctgctcaac acctcgttca
      361 acctgcgcgg ccacccgatt gtcaacaccc ccgaggaagc gtttgcgacc ttctgctcgg
      421 gcggtcttga cattctcctt ctgggtgact atctagtgaa gaaagaggag attcccgaag
      481 cgctgcggca gcgtttccgt ttggtgcagg cggaggactg aagtgtctgc gcgccgcagg
      541 agtaatctcc cgtgaggcaa tcggttatgg ccggacgagt caaaatcctc gccgagttct
      601 ggtacttcct ccggacctcc cgaaagtggt ggctcgggcc gatcctggta ttgctcgtgc
      661 tggtgtcggc cctgatcctc ttcaccgaat cctccgctct cgcccccttt atctactcgc
      721 tcttctgacc gcggggccgg ctgctctccc cctgacaaac atccccgcgc ggcatatatt
      781 ggtttcatgc cgccgcgaga cacttatccg gtgctcttcc ggaccgccgc cgtgctcctg
      841 tcgctggcgc tctttgtcgc gctcgcggag ggcgtcgtcc gcctcgctgg tctcgatccc
      901 tatttccaga accgcttctt cactgtcaac cgggcgctcg attatcccga gattttcgac
      961 aaggaccacg aactcttctg gcgcttccgc cccgatcaga gcgtgacctc ccgcttcttc
     1021 atcgggcgca cccaccgcat caacagcgcc ggtctccacg gccccgagat cacgccggcc
     1081 gaggggcgca cccgtgtcat cacgctgggc aactcgtgca ctttcggatg gggcgtcgcg
     1141 accgagcacg gctacccccg gctgctggat tccctcctcg gcgactcttt ccaggtcatc
     1201 aacgccggta tccccggcta cacctcgctg cagggcaagc gtttttaccg atccgatctg
     1261 tcccgccttc gccccgccat cgtcaccatc ctcttcgctt tcaacgacca ctgggcggcc
     1321 gccggcgacc gggccgacaa ggaccagcgt atgcccccgc agtggctgct cgacattcaa
     1381 aacgcgctcg gccgcctcca cacctatcgc cttctaaaga aagcgatcct ctcggcggtc
     1441 gaaccgccca tggattccct gttcgaccgc cgcacgccga tctaccgggt cgggctggag
     1501 gatttccggg cgaacctcga ggatctctgc cggatgattg tcgccgacag cgcgcggccg
     1561 atcctgctca catcgcccat ccccgacctg ccgacctatt acccgaaggg gtatcagtcg
     1621 aacctccaca aataccacga gcggtacaac gcggcgatcc gcgagatggc cgccgcccgc
     1681 ggttatgatg tggcggatct ggcggagggc tttgccggcc ggccggggtt gttcgatgat
     1741 cccctggtgg atccggccca cttcaacgac ttcggccacc gcctcgcggc cgcgatgatc
     1801 gcagcctgca tcagggacag cctctagcta cggcgcgctt tccgcgaacc ggtccagcac
     1861 cgcgatcagt ttgtcgatgt cgtcttcgtt gttgaagaaa tgcaccgaaa tccggatcga
     1921 accctcccgg tgcacgcacg tgattttccg ccggtagagc tccttgtgca ggtcccggaa
     1981 ccggtcgcag gtgaaagtga aaatcgagga ccggtgtttc ggctccatcg acgaggtgat
     2041 ccggtagaac ttgtgtcccc tgatgtaatc ggccagccgg tcgatcaggg cgcgggtgtg
     2101 gcgctggatg ttgcgcgtgc ccaactcgcg gaacatcgcc gccgattccc gcattcccgc
     2161 cagattcaac tcgacgtagt agcccagctc gaaccgcgcg gccgtggcga gaaactcccg
     2221 gtcgtaccgg aacaggtcgc cgaacttctg atgccactcc acgcccagcc agctggccga
     2281 ggggggctgg aggcggtccc ggatgcgatc ggcgatatag aaaaacccgc tcccctgcgg
     2341 cgcgagcagc cacttctggc acccggtcga gaagatgtcg atccccagct tccgcacatc
     2401 cagcggctgc atccccgttc cctggatccc gtcgaccacg aagaacagcc catggtcccg
     2461 gcagaactct ccgagcgcgg cgaggtcgtt ccggtatccg tcgaaaaact gcacccacga
     2521 gatagcgagc actttcgacc gcttggtgac ggcgcggctg agctgctcca catcgacccg
     2581 ccggtctcgc gtcttcacga acctcagggt aatcccgcgc gtctgggccg ccccacggaa
     2641 tgcgtagacg agcgccggaa actcgacatc ggtcagcagc acctcgtccc cctttttcag
     2701 cggcagcccg taggcggcca cgttcagtcc gaacgtggtg ttgagcccga tcccaatctg
     2761 ggacggtcgg gcgccgatga gcgcgccgta gtcccggcgg agcgcctccc gcatggcgaa
     2821 gatgctcgcc gtgctgtcct ccgccgaccg cacccgcgcc gcaatcgtct cggccagcgc
     2881 cctctgcacc cgggtcgagt acggcccgta tgacgccgag ttgaaataga ccagctcgcg
     2941 cgtgtggggg aactcgccgc gaaccgcccg gaactgttcc gctgtcgatg tcgtcatccc
     3001 gtcctcacag actgtacagg tagccgatga tggccaggca gccgcccagt ttgagcagca
     3061 ggccgagcag gaagtacaat aaatgcggca tccagagtct cacgcgcagc gacaagccca
     3121 tgatcgcggg gactccgccg agcacgaacc cggttagggc cagggcgagc cccgtgcgga
     3181 tccccgccag cggcagcacc gccgtgaaaa atgagtacgc cagagtcggg atggcgacaa
     3241 aaaataaccc ttccatgaca tacccgacca cgaacgccgc cggcccccag gcctcgcgct
     3301 gggccggcgg aatgcccttg gccatgtcca gcttccgtcc cagccactcg tgcgccagcg
     3361 tcacgaccag caggtacacc ccgcccgcaa tgagacaata ggccatcagc gtcatgttga
     3421 tcacgtgagc catccttccc ggcgatacca ggcaaacgtc tcccgcgccc cgtccgcaaa
     3481 acctatctgg gcctgaaatc cgaattccgc ccgcgctttc gccgtcgaga cttcccacga
     3541 ggccagtagt tcgcggcact tttcccgcgt caacatcggc gtcgccccgg ccagccggaa
     3601 caatccctcc gacacggccg cgatcgcgcg aaaaaccccg cccggcaggc gcaggccgat
     3661 ccccttgcgc ccgcccgccg ccgccaccag atccaccagc cgccccatcg cgtaagcctc
     3721 ctcttcggcg atgaaatacg cctccccggc gcgcgttccc cccgtcagcg ccatcaccac
     3781 cgcccggctg agatcgtcgg cgtgcaccag ttgcagtttg cgcctgggat ctccgatcac
     3841 cggccgaagc ccgcggtggg cggcggcaaa gaacgtcagc agctcgcggt cccccggtcc
     3901 gtagacggcg gccggcggaa gcgatatcac gtgaaagcgg tcggcgaacg acaagacggc
     3961 gcgctctccg gccagcttcg agcgaccgta ggtcgtgata ggctgcgg
//
LOCUS       DAOOKS010000080         4007 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_27117_length_4007_cov_3.098684, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000080 DAOOKS010000000
VERSION     DAOOKS010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4007
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27117_length_4007_cov_3.098684"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /locus_tag="PK112_02055"
     CDS             <1..817
                     /locus_tag="PK112_02055"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HPC10864.1"
                     /translation="YLQNAEIMRRALQYSRMFNIPVLQHAEDQQLCEGAVMNESFEST
                     KLGLKGAPPEAEEIAVLRDIALARSTGGRVHFQHITTKRAIEAIRQAKKEGIRVTAEA
                     APHHLVLNDAEIAKAFNTNLRVNPPLRSESDRQALIEGLVDGTIDCIASDHAPHSEEE
                     KDCEFDLAPPGMIGLETSLALINTFLIEKGLMNWADVIRKMTVNPAHILKLPGGTLAV
                     GSSADITIIDPEKKWTVRADKFRSKSKNSPYIGWKLTGQVARTILGGRTVYQR"
     gene            1002..1982
                     /locus_tag="PK112_02060"
     CDS             1002..1982
                     /locus_tag="PK112_02060"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPC10865.1"
                     /translation="MPTLSRSRMARIAARTLANMARRRPINVSFEITYNCNARCEHCD
                     LGDYVKEPRLGPEVFADWLTRLKPAVAQISGGEPLLRKDLVDIVRAMRERDPVPVFVI
                     TTNVQIMTEKKYLQLREAGMDEFSFSLDYPDDRHSAFRHLKDNFRKMSELVPTLAAHG
                     HKDIVLACVVQSDNFRDLPKIAELAREWGVGVNFSTYNALRTGKKYYLITSPQDLAEL
                     ETIVDRLAAMQREGYPIMTSEWTMRQMIDFFRTGGHPRCRAGERFMIVNPWGKLTPCG
                     MCRDHYDDPAQIRERFSRTNECGDCYTAIRANSEKTPYRLIADALRVVRR"
     gene            2037..2951
                     /locus_tag="PK112_02065"
     CDS             2037..2951
                     /locus_tag="PK112_02065"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPC10866.1"
                     /translation="MPTPAPKAPTVLGHLGLFYAAAIWGATFFVVKGALAAVDPLILV
                     GYRFLIAGGLLLAVVIILGRNLTAHLGRFAILAVILWTLYISQTVGLQYTTASNSGFI
                     TGLFIAFVPILLRLLFRRRPTIMEVIASAVSLVGLWILTGGLRQVNVGDMLTLISAMT
                     YALHLLYSDKYLKEGLDPWVMSCQQFLLVGALSIAAGWVTGRPFAVRTAGAMWVVIFL
                     ALLPTASAFVIQMLAQRIIAPVRVSLVFAFEPVFAGVFAWTLGGEPFILRTAGGGLLI
                     FLGLILSALPDPPAFRPKRAPTARRRAT"
     gene            complement(2944..>4007)
                     /locus_tag="PK112_02070"
     CDS             complement(2944..>4007)
                     /locus_tag="PK112_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HPC10867.1"
                     /translation="AINRDNVAVPGAEVALTGEFLRAALAGAACDYRSDSAYADASSR
                     TRLYDLVSYELVVVAGETFYDNVAFSPAGGGLLDTLAYYLAIGGKLLVFGRFGPSSGN
                     TVVTIPYAATAAAWDDAYSTVFHLAERSVLPLVAGGQVSGDLVGAHPQQAGWPALRWD
                     SLRTAQQEPSIQTPFGICAGSFVTLNSSLPDVLYTYDSGTDDTLTEGKAVAWRYLGAD
                     YRYIWFDLPLSFFDEAAAIAAVHRAVNDLTGTATALPPEETLPDGFALTPNYPNPFNP
                     RTTIGYTLPRTVRVKLTVYNLLGQQVRVLVDGEQAGGRHEAVWDGTTGEGRPAASGVY
                     LYRLETPSRCAARKMLLLK"
BASE COUNT          675 a         1404 c         1261 g          667 t
ORIGIN      
        1 ctacctgcag aacgccgaga tcatgcgccg cgccctccag tacagccgca tgttcaacat
       61 cccggtcctc cagcacgccg aggaccagca gctgtgcgag ggcgccgtca tgaacgaatc
      121 gttcgagtcg accaagctcg gcctgaaagg ggcgccgccc gaggccgagg agatcgccgt
      181 gctccgcgac atcgccctcg cccgctcgac cggcggacgg gtgcacttcc agcatatcac
      241 gaccaagcgg gcgatcgagg ccatccggca ggccaagaag gaagggatcc gcgtcaccgc
      301 cgaggccgcc ccgcaccacc tcgtcctcaa cgacgcggaa atcgcgaaag cgttcaatac
      361 caacctccgg gtcaacccgc ccctccgctc ggagagcgac cggcaggcgc tcatcgaagg
      421 cctcgtggac ggcacgatcg actgcatcgc ctccgatcac gcaccccact ccgaggagga
      481 gaaagactgc gagttcgacc tggctccccc cggtatgatc gggctggaga cttccctcgc
      541 actcatcaac acgttcctca tcgagaaggg tctgatgaac tgggccgacg tgatccgcaa
      601 aatgaccgtc aacccggccc acatcctgaa actccccggc gggacgctgg cggtcggttc
      661 ctccgccgac atcacgatca tcgacccgga gaagaagtgg acggtgcggg ccgacaagtt
      721 ccggtcgaaa tcgaagaact cgccctatat cgggtggaag ctgacgggcc aggtcgcccg
      781 gaccattctc ggcggccgca cggtctacca gcgctaaggt gcggcgcagg acgccccggg
      841 ggcggcggtt cgcccccggc agtcccgcgc ggggccgcgc gtgcggtttc ccaaaatgac
      901 cttgtgatcg gggccgcgcc cgcgtacctt tgacccattg acagcggatc gcggcggccc
      961 ggccgcccgg tccgatcggt atcaaccagc aaggaggcga aatgccgacc ttaagtcgca
     1021 gccgcatggc gcggatcgct gcacggacgc tcgcaaacat ggcccgccgc cggcccatca
     1081 atgtttcgtt cgaaatcacc tacaactgca acgcccggtg cgaacactgc gacctcggcg
     1141 actatgtcaa ggaaccccgg ctcggcccgg aggtgttcgc cgactggctg acccgcctca
     1201 aaccggcagt cgcccagatc tccgggggag aaccgctgct gcgcaaggac ctcgtcgaca
     1261 tcgtgcgcgc catgcgcgag cgcgacccgg tccccgtctt cgtcatcacc accaatgtcc
     1321 agatcatgac cgagaagaaa tacctccagc tccgggaggc cggcatggac gagttctcct
     1381 tctcgctgga ctatcccgac gaccgccaca gcgccttccg ccacctcaag gacaactttc
     1441 gcaagatgag cgagttggtc ccgacgctgg cggcccacgg ccacaaggac atcgtgctcg
     1501 cctgcgtcgt gcagtcggac aacttccgcg accttccgaa aatcgccgag ctcgcgcggg
     1561 aatggggggt gggggtgaat ttcagcacct acaacgctct gcgcaccggg aagaagtact
     1621 acctgatcac gtcgccgcag gatctcgccg agctggagac gattgtcgac cggctggcgg
     1681 cgatgcagcg ggagggctac ccgatcatga cctccgaatg gacgatgcgg caaatgatcg
     1741 actttttccg taccggcggc cacccccgct gccgcgccgg ggaacggttc atgatcgtca
     1801 acccgtgggg gaagctgacc ccgtgcggca tgtgccgcga ccactacgat gatccggcgc
     1861 agatccgcga gcggttcagc cgcacgaatg agtgcggcga ctgctacacg gcgatccgcg
     1921 ccaattccga aaaaaccccc taccggctga ttgccgatgc cctccgggtg gtgcggcgct
     1981 aggcccggca ctctcttaag cgcgacgtcc gcgccgccca ctctcgggtg agccctatgc
     2041 cgacaccggc gccgaaagcc cctaccgtcc tcggccacct cggcctcttc tacgccgccg
     2101 ctatctgggg ggcgaccttt ttcgtcgtca aaggggcgct cgccgccgtc gatcccctca
     2161 tcctggtcgg gtaccgcttc ctcatcgccg gcgggctcct gctggcggtt gtgatcatcc
     2221 tcggacggaa cctcacggcc catctcggac ggttcgccat cctcgccgtg attctctgga
     2281 ccctctacat ctcgcagacg gtcggcctgc agtacaccac ggcctccaac tccggcttca
     2341 tcaccggtct attcatcgct tttgtcccga tccttctgag actgctcttt cgccgccggc
     2401 cgacgatcat ggaggttatc gcttcggcgg tctcgctggt cgggctgtgg attctcaccg
     2461 ggggattgcg ccaggtcaat gtcggcgaca tgctcaccct catttcggcc atgacctacg
     2521 ccctgcacct gctgtattcc gacaagtatc tcaaggaggg cctggatcct tgggtgatga
     2581 gctgccagca gtttctcctg gtgggcgcac tgagcattgc ggcggggtgg gtgaccgggc
     2641 ggccgttcgc ggtgcggacg gccggtgcga tgtgggtggt gatcttcctg gccctgctgc
     2701 cgaccgcctc ggcgttcgtg attcagatgc tggcccagcg catcatcgcg ccggtgcggg
     2761 tgtcgctggt ttttgccttt gagccggtgt ttgcgggggt tttcgcctgg actctgggcg
     2821 gggagccgtt cattctccgc acggccggcg gcggtctgct catattcctc ggcctcattc
     2881 tctccgcctt gcccgatccc ccggctttcc ggccgaaacg cgcgcccacc gcccgccggc
     2941 gggctacttg aggaggagca ttttcctggc ggcgcagcgg gagggggtct ccaggcggta
     3001 gaggtagacg ccgctggccg ccggccgccc ctccccggtt gtcccgtccc agacggcctc
     3061 atgccgtccc cccgcctgct ccccatccac cagcacccgc acctgctgcc cgagcagatt
     3121 gtacaccgtc agcttcaccc gcaccgtccg gggaagcgtg tacccgatgg tcgtgcgcgg
     3181 attgaacgga ttggggtaat tgggcgtcag ggcgaatccg tccggcagcg tctcctccgg
     3241 cggcagagca gtcgccgtgc cggtcaggtc attgaccgcc cggtgcaccg ccgcgatcgc
     3301 cgccgcctcg tcgaagaacg acaagggcag gtcaaaccag atgtagcggt agtccgcccc
     3361 caggtaccgc caggccaccg ctttcccctc cgtcagcgtg tcatccgtgc ccgaatcgta
     3421 ggtgtagagg acatcgggca gggaagagtt cagcgtcaca aacgaccccg cgcagatgcc
     3481 gaaaggtgtc tggatcgagg gctcctgctg cgccgtccgc agcgaatccc accgcagcgc
     3541 cggccagccc gcctgctggg gatgggcccc gaccagatcc ccggagacct ggcctcccgc
     3601 caccaggggc agcaccgacc gctcggcgag gtggaacacg gtgctgtagg cgtcgtccca
     3661 ggcggccgcg gtggccgcgt aggggatggt caccaccgtg ttgcccgaac tcggaccgaa
     3721 ccggccgaag acgagcagct tcccgccgat cgcgaggtag taggccaggg tgtcgagcag
     3781 tccgcccccc gccgggctga aagcgacatt gtcgtagaac gtctctccgg ccaccaccac
     3841 cagttcgtag ctgacgaggt cgtagagccg ggtgcgggag gaggcgtcgg cgtaggcgga
     3901 atcggagcgg tagtcgcagg cggcgccggc cagggcggcg cggaggaact ccccggtgag
     3961 cgcgacctcg gctccgggaa ccgcgacatt gtcgcggttg atcgcca
//
LOCUS       DAOOKS010000081         4001 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_27164_length_4001_cov_3.209579, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000081 DAOOKS010000000
VERSION     DAOOKS010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4001
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27164_length_4001_cov_3.209579"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1197
                     /locus_tag="PK112_02075"
     CDS             <1..1197
                     /locus_tag="PK112_02075"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPC10868.1"
                     /translation="KKRITLTLIALGWGTISIVLLLAFGEGLQRQMSINGKGLGEGIA
                     IVWGGQTTIPFKGYGKGRPIWLKKDDVRYLEERVPGLRYIGGEYHRWGTEIRYGDKVL
                     SEHANGIPPNYEFMRSHIPMQGGRMINDLDMRLKRRVAYLGHNLKERLFGDEDAVGKQ
                     ILVKGIPFTIVGVEIEKMQMSSYSGQDADKLSLPATTFEAIFGDRYLDNIVYQPERVE
                     DMKAVEKAVYEAMAAKYTFDPADERALWVWDVAESARQFENLMIGLKLFLGIVGGLTL
                     LIAGVGVANIMYVSIRERTREIGIKMAVGARRSYILAQFLIEAIIITFFGGFGGLAIS
                     YILTEGFKRLPIESEVLDFMGRPTISLDVGAIVVAILGVMGLLSGFFPALRAASVNPV
                     ESLRYE"
     gene            1225..1935
                     /locus_tag="PK112_02080"
     CDS             1225..1935
                     /locus_tag="PK112_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007083985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPC10869.1"
                     /translation="MLIALHNVEKEYRTGAVSFKALKGVSLAVDRGEYLAIVGPSGSG
                     KSTLMNLIGCLDVPSAGEYLLDGRPVGAMTSNQLADIRNQTIGFVFQAFNLLPYATSF
                     ENVEVPLLFARVPARQRKRRVLELLARVGLADKVSNKPTELSGGEMQRVAIARALANE
                     PEIILADEPTGNLDTASGKQIIALFDELRNAGKTVIIITHDVNIAKQAPRIVRIRDGA
                     IEENGYHPVGVNVQSLQG"
     gene            complement(2010..2657)
                     /locus_tag="PK112_02085"
     CDS             complement(2010..2657)
                     /locus_tag="PK112_02085"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HPC10870.1"
                     /translation="MISWREIGRLPNLLSLSRIAMTPLIGYFLWLDTPAAPFVCVGFL
                     FLAAVTDGLDGYLARRRGLTSDLGRVLDPVADKIMAGVLVLLLVAYRGFPLWLAVVIL
                     GRDLLIMAAGLFLLQGRKVVLGSNITGKYTFTAIAFLLGSYVIRFPFGMLVMPPITLA
                     LIAFSMILYARVFLRVRRGDRPAPEHDRPGYKMVRVTLLVLFLVIFAVQLAEYLW"
     gene            3027..>4001
                     /locus_tag="PK112_02090"
     CDS             3027..>4001
                     /locus_tag="PK112_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10871.1"
                     /translation="MIVPRILAAAILGVLLLAAPQAAAQLCGDVDNSGGVSLGDLAAL
                     VNWLGIEGAPPLPNPAMAEMDGRAGITVGDLVPLLEYVFEGGPVPTCGATDTYTFAPS
                     PGDTLFIPRMLGIAAEVNEVTLPLTIHVQSGVRAFNLSFLENTAGPAFRLADVTYPPE
                     VGLLLVGRDYTNHDSCRAIGLEFNPNPFTGTIVVANLIYERYSSGVADIIPVPVNRAD
                     PYRNAVARDGDLYVPTVAYLDAARPPDTLFAAPTALDFDAVYGYPAVDTLPVSFTSAP
                     AAVDFTISPDESWIRVVNGAESPWTAPATVQVTVDLAGLPGGLHTGHLN"
BASE COUNT          753 a         1307 c         1259 g          682 t
ORIGIN      
        1 aagaaacgga tcacgctgac gctgatcgcg ctcggctggg ggacgatctc gatcgtgctg
       61 ctgctggcgt tcggcgaggg actccagcgg cagatgtcga tcaacggcaa ggggctgggc
      121 gagggcatcg cgatcgtctg gggcggccag acaacgatcc cgttcaaggg atacggcaaa
      181 gggcgcccga tctggctgaa gaaggacgac gtgcggtacc tggaggagcg cgtgcccgga
      241 ctccgctaca tcggcgggga gtaccaccgc tgggggaccg agatccgcta cggcgacaag
      301 gtgctgagcg agcacgccaa cggcattccg cccaactacg aattcatgcg ttcgcacatc
      361 cccatgcagg gggggcggat gatcaacgac ctcgacatgc ggctcaaacg ccgggtcgcc
      421 tacctcggcc acaatctcaa ggagcggctg ttcggcgacg aggatgcggt cggcaagcag
      481 attctcgtca aggggatccc cttcaccatc gtcggcgtcg aaatcgagaa gatgcagatg
      541 agttcctaca gcggccagga tgccgacaag ctctctcttc ccgccaccac gttcgaggcg
      601 attttcggcg accgctacct cgacaacatc gtgtaccagc ccgagcgcgt cgaggacatg
      661 aaagcggtgg agaaggccgt gtacgaggcg atggcggcga agtacacgtt cgacccggcc
      721 gacgagcgcg ctctgtgggt gtgggacgtc gccgagtccg cccggcagtt cgagaacctc
      781 atgatcgggc tgaagctttt cctcggcatt gtcggcggac tgacgctgct catcgccggg
      841 gtcggcgtgg ccaacatcat gtacgtgtcg atccgggagc ggacgcggga gatcggcatc
      901 aagatggcgg tgggggcgcg gcggtcgtac attctcgccc agttcctcat cgaggcgatc
      961 atcatcacgt tcttcggcgg cttcggcggc ctggcgatca gctacatcct caccgagggt
     1021 ttcaaacgcc ttcccatcga atcggaggtg ctggatttca tgggacggcc gaccatctcg
     1081 ctggacgtgg gcgcgattgt cgtcgccatc ctcggcgtca tgggcctgct gtcgggcttt
     1141 ttccccgcgc tgcgggcggc ctcggtcaac ccggtggagt cgctgcgcta cgagtaacca
     1201 ttggagacag ggagcacaaa ccacatgctg atcgcactgc acaacgtcga gaaggaatac
     1261 cgcaccgggg cggtgtcgtt caaggccctc aagggggtgt cgctggcggt cgaccggggc
     1321 gaatacctcg ccatcgtcgg gccctccggc tccggcaagt cgaccctcat gaatttgatc
     1381 ggctgcctgg atgtgccgag cgcaggcgag tatcttctcg acggccggcc ggtcggcgcc
     1441 atgacctcca atcagctcgc cgacatccgc aaccaaacca tcggcttcgt cttccaggcg
     1501 ttcaacctgc tgccctacgc cacgtcgttt gagaacgtcg aggtccccct cctgttcgcc
     1561 cgggtgccgg cgcggcagcg caaacggcgg gtgctcgagc tgcttgcccg ggtcggcctc
     1621 gccgacaaag tcagcaacaa gccgaccgag ctctccggcg gcgaaatgca gcgtgtggcg
     1681 atcgcccgcg cgctggctaa cgagccggag atcatcctcg ccgacgagcc gaccggcaat
     1741 ctcgacaccg cttccggaaa acaaatcatt gccctgttcg acgagctccg caacgcaggc
     1801 aagacagtca tcatcatcac gcacgatgtc aacatcgcca aacaggcgcc gcgcattgtc
     1861 cgcatccgcg acggggcaat cgaggagaac ggctaccacc cggtgggggt gaacgtccaa
     1921 tccctccagg gttaagcggc tgcctccgat gaccgccttt ttcggaacag gctatccggt
     1981 cccgattctg ccggcgcctt cagccggaat taccaaaggt attcggcgag ttggacggcg
     2041 aagataacca gaaagagcac cagcagagta acgcgcacca tcttgtagcc cgggcggtcg
     2101 tgctcggggg caggccgatc gcctcggcga acccgcagga acacgcgggc atagaggatc
     2161 atcgaaaagg caatgagggc gagcgtaatc ggcggcatca ccaacatgcc gaacgggaag
     2221 cggatgacat agctgcccag caggaaggcg atcgcggtga aagtgtattt cccggtgatg
     2281 ttggagccga ggacgacctt ccgcccctgc agcaggaaga gcccggcggc catgatgagc
     2341 aggtcccggc caagaatgac caccgccagc cagagcggaa aaccccggta ggcgaccagc
     2401 aggagtacca acaccccggc catgatcttg tcggccaccg gatcgagcac ccggccgagg
     2461 tcgctggtga gcccccgccg ccgcgccagg tagccgtcga gtccgtcagt cacagccgcc
     2521 aggaacagga aaccgacgca gacaaacggg gcggccgggg tgtcgagcca caggaaatag
     2581 ccgatcagcg gggtcatcgc gatccgcgac agactcagca ggttgggcag gcgccctatt
     2641 tcacgccagg aaatcacact cttttcgcca cgtttcgggt tgtccgggaa actaacgccg
     2701 ccggtgaatc ttcgcaaggg gtatgaacgg ccgccgggcg ggctaaagca ttccgcgccg
     2761 cggccgatac cactactacc tccttttgag agaccctttc gttaacagtt gcaggtgcgc
     2821 cgccgcgccc cgccgggcgc ggcgttcttc tgccggcggg atcgcttccc cggccttcgc
     2881 agtgcgcaac tatcgcttga caaacggcac ctttgcctgc atgttaaagt agtcatcggg
     2941 gatcgcccga ccctgctcac acaagcgccg atccccttac tcaaaccatt tatcatgagc
     3001 gcgctttcgc gcaggaggat actgtgatga tcgtgccgcg aatattggcg gcagccatcc
     3061 tcggcgtgct gttgctcgca gcgccccagg cagcagcaca gctgtgcggc gatgtggaca
     3121 acagcggggg cgtcagcctt ggagacctcg ccgctctcgt caactggctc ggcattgagg
     3181 gtgccccgcc gttgcccaac ccggccatgg ccgaaatgga cggccgcgcc ggcataacgg
     3241 tgggcgatct agtgccgctg ctcgaatacg tcttcgaggg cgggccggtc cccacgtgcg
     3301 gcgccacgga tacctacacc ttcgcccctt cgccgggcga cacgctgttt atcccccgca
     3361 tgctcggcat tgcggcggaa gtcaatgagg ttacgctccc gctgacgatc cacgtgcagt
     3421 cgggggtgcg ggccttcaac ctgtcatttt tggaaaacac cgctggaccg gccttccgac
     3481 tggccgacgt cacctacccc ccggaagtcg ggctcctcct cgtgggccgg gactacacga
     3541 atcacgattc gtgccgggcc atcggccttg agttcaaccc gaacccattc acgggcacca
     3601 tcgtcgtcgc caatctgatc tatgagcgct attcgtcggg agtggccgac atcattcccg
     3661 tcccggtcaa ccgggccgat ccgtaccgca acgccgtggc gcgcgacggc gatctgtacg
     3721 tgccgaccgt cgcgtatctc gatgcagccc ggccgcctga cacgctgttc gccgctccca
     3781 cggcgcttga tttcgacgcc gtctacgggt atccggccgt cgacacgctg ccggtcagtt
     3841 tcacctcggc gccggctgcg gtcgacttca cgatctcccc ggacgagagc tggatacggg
     3901 tcgtgaacgg cgccgaatct ccgtggaccg ccccggcaac ggtgcaggtg acggttgatc
     3961 ttgccggtct ccccggcggc cttcacaccg ggcacctgaa c
//
LOCUS       DAOOKS010000082         3984 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_27327_length_3984_cov_3.917282, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000082 DAOOKS010000000
VERSION     DAOOKS010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3984
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27327_length_3984_cov_3.917282"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="PK112_02095"
     CDS             complement(<1..297)
                     /locus_tag="PK112_02095"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HPC10872.1"
                     /translation="MTTDQPQTPDEAKQAAGPRILPILPLKGTIVYPYLVVPLMIQDA
                     EQARLVDEALMRGSRIGLFLQKDAEQEHPGPDDLYHTGCSGSILKMLRFPDGTVR"
     gene            complement(407..1459)
                     /locus_tag="PK112_02100"
     CDS             complement(407..1459)
                     /locus_tag="PK112_02100"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HPC10873.1"
                     /translation="MKLGIIGKPQAGKTTIFNAASGQQEAVGDFSQAAHRAIIKVPDP
                     RLAVLAEIEDPKKITPAEIEFLDAPGFTGKGKEAGAVEISQEVRLMEALILVVDAFSP
                     DAAPERDLRNLTEEMILSDLSIVEGNLDKKGRKMKLTGDKSATHDLELLETCRAQLEG
                     EQPLIDLDMAEEDLKALRGYSFLTLKPLLIAVNIAEDALDRAGAMREGYAGLAAPGKR
                     DVAVVCGRIEAELAALPEIERAAFLADLGIATPAVDQVIRKSYDLLGLISFLTAAKPE
                     VRAWTIRAGTTAQRAAGVIHSDIERGFIRAEVTRFEDYARYRTPAALKAAGKTRLEGK
                     DYVVQDGDVILFRFNV"
     gene            complement(1780..2631)
                     /locus_tag="PK112_02105"
     CDS             complement(1780..2631)
                     /locus_tag="PK112_02105"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HPC10874.1"
                     /translation="MQFGAHESIAGGVFTAIERGHRATCDTIQMFNKSNNQWKAKKLT
                     TEEIDLFFARIEETGVTAAVSHSSYLINIASPDEALAEKSYASLKEEMERCRTLRIPA
                     LVLHPGSHVGSGEEIGMQRVALSINRLFDELPDNTVTLLLETTAGQGSNLGCTFEQLA
                     YMIDRVDNKAQIGVCLDTCHIFAAGYPIVEPADYRKTMKQFDEVIGWERLKVIHANDS
                     LKELGSRRDRHEHIGRGHIGLKGFGNFINDRRLKKIPMIIETPKGDDLTEDIENLKVL
                     RGLVKKK"
     gene            3094..>3984
                     /locus_tag="PK112_02110"
     CDS             3094..>3984
                     /locus_tag="PK112_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10875.1"
                     /translation="MCTFLSVDEAAFTDTHWRQFHSFLVNMHARGFGNVPPSTAQALR
                     QRIEARLALRDGFREWVIVKDSAVVGWLGIHVRPGSETAPPVMFLVFDFVPGLEVAPL
                     SARAAGLVAAAMRENRCGEVHCMAAEAHLDSLRQTWGGRILSRLHRYRLDRRTANRDL
                     INEWLAEFPRQFPSLRIEIFTEIPERHLDSWIALFGQFLADMPNEGTAAAPFTMTRAE
                     VRKFEALRRRNGQYLYTGALLDGEGRMIGHSNMATHDDPPTEWHQAMTGIERAWRGRG
                     LSKWLKAALFVRTGEDFPQSR"
BASE COUNT          668 a         1245 c         1284 g          787 t
ORIGIN      
        1 ccgcaccgtg ccgtccggga accgcagcat cttgagaatg cttccggagc agccggtgtg
       61 gtagagatcg tcggggccgg ggtgttcctg ttcggcgtcc ttctggagga agagcccgat
      121 ccgcgagccg cgcatgaggg cctcgtcgac gaggcgggcc tgctcggcat cctgtatcat
      181 cagcggcacc acgaggtacg gatagacgat ggtgcctttc agcgggagaa tgggcaggat
      241 gcgcgggccg gccgcctgct tcgcctcatc cggagtctgc ggttggtcgg tcgtcatggt
      301 cacaatacct cattcggtga cgccccgcgg ggcggggcgg gctgttgtcg cactttcccg
      361 cgcggccggc ccggccgcgg cacgggcggg gcgcgtttcg gcatccctac acgttgaagc
      421 ggaacaggat cacgtcgccg tcctgcacca catagtcctt gccctcgagg cgcgttttgc
      481 cggccgcttt gagcgccgcc ggcgtgcggt accgggcgta gtcctcgaac cgcgtgacct
      541 cggcccgaat gaagccgcgc tcgatgtcgc tgtgaatgac gccggccgcc cgctgggccg
      601 tcgtgccggc gcggatcgtc cacgcccgca cctccggctt ggcggccgtg agaaacgaaa
      661 tcagaccgag caggtcgtag gacttccgga tcacctggtc gaccgccggc gtcgcgatcc
      721 cgaggtcggc caggaacgcg gcccgctcaa tctcgggcag cgcggccagc tcggcctcga
      781 tccggccgca cacgaccgcc acatcccgct tccccggcgc cgcgaggccg gcatacccct
      841 cgcgcatcgc cccggcccgg tccagcgcgt cttcggcgat gttaaccgcg atcaggagcg
      901 gcttgagcgt caggaaggag tatccccgga gcgctttaag gtcctcttcc gccatgtcca
      961 gatcgatgag cggctgctcc ccctcgagtt gggcgcggca cgtttcgagc agctcgaggt
     1021 cgtgcgtcgc cgacttgtcc ccggtcagtt tcatcttccg tcccttcttg tcgagattgc
     1081 cttcgacaat ggagaggtcg gagaggatca tctcctcggt caaattccgg agatcgcgtt
     1141 ccggcgcggc gtcgggcgag aaggcatcga ccaccaggat gagcgcctcc atgaggcgga
     1201 cctcctggct gatctcgacg gctccggcct ctttcccctt gccggtgaac ccgggagcgt
     1261 cgagaaattc gatttccgcc ggcgtgatct tcttgggatc ctcgatctcc gccagcacgg
     1321 ccagccgggg gtcgggcact ttgataatcg cccggtgggc cgcctgactg aaatcgccga
     1381 ccgcctcctg ctgtccggag gcggcgttga aaatcgtcgt cttccccgcc tgcggtttgc
     1441 cgatgatgcc gagtttcata gagcgtaatt aagggtgggg cgaaggcgaa ggcaaggctg
     1501 attccggccg gtcaggaggt tcgcccgctt ccgctcggat ctcatcggat ttccgccggg
     1561 gccggacggc acggcaatcg tgtgaaagtg ccgctgtgtc cgccggctgc ggctctggat
     1621 aacggggacc acatcgccca cgttgcgcgg gacaggtcgg gcgaaggggg aaagcgaccg
     1681 cctgggacga tagaccgcag gcacgggctc ttctgctgcg atagttgcgg agtgggggca
     1741 gcccggttaa gtggtgaagg ggaaccggct gcgccggtcc tacttcttct tcaccaaacc
     1801 gcgcagcact ttcagattct cgatatcctc cgtcagatca tcgcctttgg gcgtctcgat
     1861 gatcatgggg attttcttta accgcctgtc attaatgaag ttaccgaatc ccttcaggcc
     1921 gatatgcccc cgcccgatat gctcgtggcg gtcccgcctg gacccgagct ctttcaggct
     1981 gtcgttggcg tggatcactt tcaggcgttc ccaaccgatc acctcgtcga actgcttcat
     2041 cgttttccgg tagtccgccg gttcgacaat cgggtaaccg gccgcgaaga tgtggcaggt
     2101 gtcgagacac acgcctatct gagccttgtt gtcgacgcga tcgatcatat atgccagttg
     2161 ctcgaaagtg cagccgagat tgctcccctg cccggctgtc gtctccagga gcaacgttac
     2221 cgtgttgtcc ggcagttcgt caaagagacg gttgatggag agggcgacgc gctgcatccc
     2281 gatctcttcc ccgctcccga catgcgagcc ggggtggagc acgagagccg ggatccgcag
     2341 cgtgcggcag cgctccattt cctctttgag cgaggcgtat gatttctcgg cgagggcttc
     2401 atcgggggaa gcgatgttga tcaggtagga ggagtgcgat accgccgcgg tgacgccggt
     2461 ttcttcgata cgggcgaaga acaggtcgat ctcttcggtg gtgagttttt tggctttcca
     2521 ctggttgttc gatttgttga acatctggat cgtatcgcac gtggcccggt ggccgcgctc
     2581 aatggcggtg aagacgccgc cggcaattga ctcgtgagct ccgaactgca tgtggccctc
     2641 gctggttgat ggtcaggtcg gaaaaacgcc cgatgaacat accggttttt ttcggcacgc
     2701 gcaagccgcg ggcgagcctg atctgacata agcggctatt aatcaatttg ttacgccgaa
     2761 gtcacgagag ctcgagagtg agaaaagaca caaaataggt gaaaaatctt tttttgaagt
     2821 gacagaatat gacacaatgg cggtgtacac tactatcggt cttttgaccg acctgtccca
     2881 accaaggtgg accgaatccc ttagtagggc acaccggggc ggggtcgcgt tgcgaccctg
     2941 ccctttatct atcgccgcat ctcgtccccc gaaaggctct taaggtgccg gcctatcctt
     3001 tcacggggcg gtgcgaatcc caattgccgc tctcccccac tccccgcata ttaactcccg
     3061 ataaccagtg ctgacaggga gctaccgaag attatgtgca catttctgtc cgtcgacgag
     3121 gccgcattca ccgacactca ctggcggcag ttccacagtt tcctcgtgaa catgcacgca
     3181 cggggattcg ggaatgtgcc gccgtccacc gcccaggcgc ttcggcaacg catcgaagcg
     3241 cgcctggcgt tgcgggacgg attccgcgag tgggtaatcg ttaaggattc agccgttgtc
     3301 ggctggttgg gtattcatgt ccggccgggt tcggagactg cgccgccggt gatgttcctc
     3361 gtgttcgact tcgtgccggg gctggaggtc gcgccgctct cggcccgcgc ggcgggcctg
     3421 gttgccgccg cgatgcggga gaaccggtgt ggcgaggtcc actgcatggc ggcggaggcc
     3481 cacctcgaca gcctgcggca gacctggggc gggcgaattc tcagccggct ccaccgctac
     3541 cgtctcgacc gccggaccgc caaccgggac ctcatcaacg agtggctggc ggagtttccc
     3601 cgacaattcc cctcactgcg catcgagata ttcaccgaga tccccgaacg gcatctcgat
     3661 tcctggatcg ccttgttcgg gcagtttctg gccgacatgc ccaacgaggg aacggcggcg
     3721 gcgccattca cgatgacgcg cgcggaggtg cgcaaattcg aggcgctgcg gcggcgcaac
     3781 gggcagtacc tgtacaccgg ggcgctgctc gacggggagg gccggatgat cgggcactcg
     3841 aacatggcga cccatgacga cccgcccacg gagtggcacc aggcgatgac cggaatcgag
     3901 cgcgcctggc ggggccgggg gctctcgaag tggctcaaag cggccctgtt tgtgcgcacc
     3961 ggcgaggatt tcccgcaaag ccgc
//
LOCUS       DAOOKS010000083         3973 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_27430_length_3973_cov_3.937979, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000083 DAOOKS010000000
VERSION     DAOOKS010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3973
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27430_length_3973_cov_3.937979"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="PK112_02115"
     CDS             complement(<1..354)
                     /locus_tag="PK112_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10876.1"
                     /translation="MNRSAFLLGFVATGGQTLLLRELVSSLGGSELFIGSALFGWLAA
                     VAAGAWAGGRWPGRLRAALLLVLASLVLPLCVAAARLAPLAYGVTVGEAIPLVPALTI
                     SFLVMAPLGVLSGWLF"
     gene            complement(351..1493)
                     /gene="amrS"
                     /locus_tag="PK112_02120"
     CDS             complement(351..1493)
                     /gene="amrS"
                     /locus_tag="PK112_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HPC10877.1"
                     /translation="MNRRSFLRCLGCGAAATALPHADFLPDLLGGIENACAIDLSDAL
                     SSEEARHYTKLAGGGIECRLCPRRCRVTDLERGYCGVRENRGDTYHTLVYGKPCAVNV
                     DPIEKKPLNHFYPGTAAFSLATAGCNVNCKFCQNWEISQSRPEQTKNFDLPPEAAVAL
                     CRQQGAPTIAFTYSEPTVFYEYMYDIARLGRAQGIRSVMITGGYIEEAPLAELMPHLD
                     AIKVDLKAIRASYYRDVVRGELTPVLERLQQIRKAGLWLELVYLVVPTLNDSDAEFRE
                     LGQWVFRYLGADVPLHFSRFTPQYLLEHLPPTPVATLERAHAIAGAEGLRFVYVGNVP
                     GHRAESTWCPGCGALVIERRGYRTSAAGLAGDRCVACGREIPGRFK"
     gene            complement(1490..2407)
                     /locus_tag="PK112_02125"
     CDS             complement(1490..2407)
                     /locus_tag="PK112_02125"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HPC10878.1"
                     /translation="MQRVVVVKSVSFSGGRRPGPETYRRLLEVGLAHLAGLPPAAALR
                     SLLPGGTIGMKVNALVGPRNSTPPALTAAAGDLLEAAGWAAGDLIVWDRSNRELDRAG
                     FTLNVGGNGRRCFGTDTAGFGYTAEIHTYGEVGSRVSRVLTEAVDSNINLPVLKNHSI
                     VGLSGGLKNMYGAIHNPNKYHDHCGDPYCAHVSLLEPVRRKNRLTIADAVLVQYHGGP
                     GFREEHVAPYGAVLLSDDPVAVDRVGLEIIEYLRARAGLGTLAEDKRAVTYLDTAACG
                     LGAADLARIDVIVRAVDGAGRETSGELFS"
     gene            2491..>3973
                     /gene="amrB"
                     /locus_tag="PK112_02130"
     CDS             2491..>3973
                     /gene="amrB"
                     /locus_tag="PK112_02130"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HPC10879.1"
                     /translation="MTRFTVCFLLVLPAAAALAASAPPVTRPPAVAGSFYPGDSAELA
                     AMIAGQLAAAPAAAIDGRIVAIVVPHAGLVYSGPIAAHAYKALAGRPIETAILCGPAH
                     RYGFRGASVYGPGVTWQTPLGPVPCDDARIERLLGASSWLKVVPEAHLKEHGLEVQLP
                     FLQTVLPGAHIVPIAMGYPDGHTVDGLTAALVAAARPPGAVLIASTDWQHYRPAAGGY
                     PMDSLGLACLAAFDADALLRNLEAKKVEACGGAGVVAVMRAARKLGADKVKILGYGDS
                     GDLTGDKSSVVGYAAVAFYCSADGENSAAPGEAAAPEKGMEPYDLTQEERQTLLTIAR
                     RSIIGYLEDGAAPDFEAAGKLAEPGAAFVTLEKQGRLRGCIGHVVAADPLFRTVAACA
                     VQAAVGDPRFEPVTRSEIDQLTIEISVLTPLQAVTIFEEIEVGRDGLIISLGPNRGLL
                     LPQVATEYGWTREEFLRQTCRKAGLSPDAYKSPQAKVERFQALV"
BASE COUNT          654 a         1433 c         1308 g          578 t
ORIGIN      
        1 aaagagccag cccgagagca ccccaagggg agccataacg aggaaggaga tggtcagggc
       61 cggcacgagc gggattgcct ccccgaccgt cacgccgtaa gcgagcggcg cgaggcgggc
      121 agcggccacg cagagcggga gcacaagcga ggcgaggacg agcaggagcg cagcccgcag
      181 gcggccgggc catcgacccc ccgcccaggc tcccgccgcg accgccgcca gccacccgaa
      241 gagcgcgctc ccgatgaaca gctcgctccc cccgagcgag gacaccagtt cccgcagcag
      301 gagggtttgt ccgccggtgg ctacgaaacc caacagaaac gccgaacggt tcatttgaac
      361 cgtccgggta tttccctccc gcaggccacg caccggtcgc cggccaggcc cgcggccgag
      421 gtgcggtacc cgcggcgctc gatcaccaag gcgccgcaac cggggcacca ggtcgactcc
      481 gcccggtggc cgggaacgtt gcccacatat acgaagcgca atccctcggc gccggcgatc
      541 gcgtgcgccc gctcgagcgt cgccaccggt gtcggcggaa ggtgctccag gaggtactgc
      601 ggcgtgaaac gcgaaaagtg aaggggaaca tccgcgccca gataccggaa aacccactgg
      661 ccgagttccc ggaactcggc gtcgctgtcg ttgagcgtcg gcaccaccag gtagacgagt
      721 tccagccaca gcccggcctt gcgaatctgc tgcagacgct cgagcaccgg cgtgagctcc
      781 ccgcgcacca cgtcgcggta gtaggacgcg cggatcgcct tgaggtcaac tttgatcgcg
      841 tcgagatgcg gcatcagctc ggccagcgga gcctcttcga tgtacccgcc cgtgatcatc
      901 accgaccgga tcccctgcgc ccggccgagc cgcgcgatgt cgtacatgta ctcgtagaac
      961 accgtcggct cgctgtaggt gaaagcgatc gtcggcgcgc cctgctgccg gcagagcgcc
     1021 accgccgcct ccggcggcag atcgaaattc ttggtttgct ccgggcggga ctgcgaaatc
     1081 tcccagttct ggcagaactt gcagttgacg ttgcacccgg cggtggccag agaaaacgcc
     1141 gccgtgcccg ggtagaaatg attgagcggt ttcttctcga tcgggtcgac attcacggcg
     1201 cagggcttgc cgtacacgag cgtgtgatag gtgtcccccc ggttctcccg caccccgcag
     1261 tagccgcgct cgaggtcggt gacgcggcag cggcgcgggc agagcctgca ctcaatgccc
     1321 ccgcccgcca gcttcgtata gtggcgcgcc tcctccgacg acagcgcgtc gctcaggtcg
     1381 atggcgcagg cattctcgat ccccccgagg aggtcgggga ggaagtcggc gtgcgggagc
     1441 gccgtcgccg ccgccccgca gccgaggcag cgcagaaagc tccgccggtt catgagaaca
     1501 actccccgct ggtctcccga ccggccccat cgacggcgcg cacgatcacg tcgatccggg
     1561 cgaggtccgc ggcgccgagc ccgcaggcgg cggtatcgag ataggtcacc gcccgcttgt
     1621 cctccgccag cgtccccagc cccgcccgcg cgcgcaggta ttcgatgatc tccaggccga
     1681 cccggtcgac cgccaccgga tcatccgaca gcaggaccgc cccgtacggc gccacgtgct
     1741 cctcgcggaa acccgggccg ccgtggtact gcacgagcac cgcatcggcg atcgtcagcc
     1801 ggttcttccg ccgcaccggc tccagcagcg agacatgcgc gcagtatgga tccccgcagt
     1861 ggtcatgata cttgttggga ttgtgaatcg cgccgtacat gttcttcagc ccgcccgaga
     1921 gaccgacaat ggagtggttc ttgagcaccg gcaggttgat gttcgagtcg acggcctcgg
     1981 tgagcacgcg gctcacccgc gagccgacct ccccgtacgt gtggatctcg gcggtgtagc
     2041 cgaagccggc ggtgtcggtc ccgaaacagc gccgcccatt tccccccacg ttgagagtga
     2101 aacccgcccg gtcaagctcg cggttggagc ggtcccacac gatcaggtcg ccggcggccc
     2161 agccggccgc ttcgagaagg tccccggcgg cggcggtgag cgcgggcggc gtcgaattgc
     2221 gcggcccgac cagcgcgttc accttcatcc cgattgtgcc ccccggaagc aggctgcgca
     2281 gcgccgccgc cgggggaagg cccgccaggt gcgccaggcc gacctccagc aggcgccggt
     2341 aggtctccgg acccggccgc cgtccgccgg aaaaagatac tgacttgacc acaacgacgc
     2401 gctgcatatc ttaataatat acggcccgcc ggagaacgac aaattctttc cgcccggcgg
     2461 gcccgcaccg ccggagatcg gggagtgctc atgacacgct tcaccgtttg tttcctgctc
     2521 gtgctgccgg cggcggctgc tctggccgca tccgcgccgc ccgtcacccg cccgcccgcg
     2581 gtcgccggtt ccttctaccc cggcgacagc gcggagctgg cggccatgat cgccggccag
     2641 ttggccgccg cccccgcggc cgccatcgac ggccgcatcg tggccatcgt cgtcccccat
     2701 gcggggctcg tgtattcggg gccgatcgcc gcgcacgcct acaaagcgct cgcgggacgg
     2761 cccatcgaaa cggccatcct ctgtggcccc gcccaccgct acgggttccg cggcgcctcg
     2821 gtttacgggc cgggagtgac ctggcagacg ccgctgggcc cggtgccgtg cgatgacgcc
     2881 cgcatcgaac gcctcctcgg cgcctcctcc tggctcaaag tcgtccccga ggcccacctc
     2941 aaggagcatg ggctcgaggt gcagctcccc ttcctccaaa ccgtcctccc cggcgcgcac
     3001 atagtcccga tcgctatggg ctaccccgac ggccacacgg tcgatgggct gaccgcggcg
     3061 ctcgttgccg ccgcccggcc ccccggggcc gtgctcatcg cctcaaccga ctggcagcac
     3121 taccgcccgg ccgccggcgg gtacccgatg gattcgctcg gcctggcctg cctcgcagct
     3181 ttcgatgccg acgcgctgct gcgcaacctc gaggcgaaga aggtcgaggc ctgcggcgga
     3241 gcgggggtcg tggccgtaat gcgcgccgcc cgaaaactcg gcgccgataa agtgaaaatc
     3301 ctcggctacg gcgactccgg cgatctcacc ggcgacaaga gctcggtcgt gggatacgcg
     3361 gccgtcgcct tctattgcag cgccgacggc gagaactcgg ccgcgccggg tgaagccgct
     3421 gccccggaaa aaggtatgga accatatgat ctgacccagg aggagcggca gacgctcctg
     3481 acgatcgcgc gccgctctat catcgggtat ctcgaggacg gcgcggcacc ggatttcgag
     3541 gcggccggca agctggccga acccggcgcc gccttcgtga cgctcgagaa acagggccgg
     3601 ctgcgcggat gcatcgggca tgtcgtcgcc gccgatcccc tcttccggac ggtggcggcc
     3661 tgcgccgtgc aggcggccgt cggggacccg cggttcgaac ccgtcacccg gtccgaaatc
     3721 gatcaactca cgattgagat ctcggtcctc accccgctgc aggcggtgac aatattcgag
     3781 gaaatcgagg tcgggcggga cggcctgatc atttcactcg gcccgaaccg cgggcttctg
     3841 ctgccgcagg tggcgacaga gtacgggtgg acacgggagg agtttctgcg ccagacctgc
     3901 cgcaaagcgg gcttgtcgcc cgatgcctac aagtcgccgc aggcgaaagt ggagcggttt
     3961 caggcgctcg tgt
//
LOCUS       DAOOKS010000084         3971 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_27459_length_3971_cov_2.562819, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000084 DAOOKS010000000
VERSION     DAOOKS010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3971
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27459_length_3971_cov_2.562819"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="PK112_02135"
     CDS             complement(<1..496)
                     /locus_tag="PK112_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10880.1"
                     /translation="MGRLKEFEYSFKRFFYRATASLLRPRPVGEGPLDGAALRKVLFL
                     RPDKLGDMVISFPVFDALRSRYPHLRIGVLASPRNSAITLGDPRFDEIYMYTKRAPHD
                     LRELRRLRRAQYDCVVDLVYGDSVTTLYLSEYVARGKPRIGVGKERYARYYDFNRSGR
                     GQILE"
     gene            complement(483..1616)
                     /locus_tag="PK112_02140"
     CDS             complement(483..1616)
                     /locus_tag="PK112_02140"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPC10881.1"
                     /translation="MHILQLDSIEPETYGGMEEWIRLVSGGLAARGHRVTVVGRTGAE
                     FLRRVELDTRGVETLHLAIAGDFGPLVIARLRTFMRQRAVDIVTVNFNKDIRLAGIAA
                     RWAGRPRVIWRVGLDIAGDRLRHRLLTPRLVDAVITPSAALKKQIVAHGYISPEMVTV
                     IPNGIPDRPVHEQRPQAARRLREKYGLPPAALVAVTSGRFVDQKGHAYLVAAAGEIIR
                     QAPDIRFLWLGDGPLETRLKAAIADAGLESFFVFAGMLRDFDLELAGSDFMLHPAVEE
                     PFGFVLIEAMRAGLPVAATRVGGIPEVVAEGSSAVLFPPRDPAALAAAAVALACDSDA
                     RTRLGRAGRERFEQRFSADRLVDDVERFFLSQLEEAGQSHGTA"
     gene            complement(1597..3513)
                     /locus_tag="PK112_02145"
     CDS             complement(1597..3513)
                     /locus_tag="PK112_02145"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPC10882.1"
                     /translation="MNLYRRALAALKPYWKHLVTASVSAALSAVLAGLLVWMLGPLLM
                     VLFEVSDPMATVAAPTATAEVRPATPGEATGNSGMIQKLSSRLGKVKESMQAWVDGIV
                     RSDNRRQTLLNFCWLAFLVVIAKNVFLYLRGYFMAYVQQALVRRFRDQLFEKYQRLPL
                     GYFHGRRTGQVISRITNDVLVLNDSVDIGFNNLVTDAITVIVLFAFLVILSWKLTLLS
                     MIVMPLVFVFIWFVGKKLRKYSARTQERMADVNSVLEEAVTNLRIVKAFSTEEFETGR
                     FFRATHQYFRSLLRMTRIRHLAAPINDMLATTAGLFILLVAGTRIIAGTGELSAGDFI
                     TFIVAMLAMIKPVKSLSQIHIKLQEGMAAAERIFEVLDAPETVGEPAAPVVLPEFREV
                     IRYEGVWFRYETSDDVLRDVTFEVRKGEVVAIVGPSGAGKSTLLDLLPRFYDPQRGRV
                     AIDGHDIRTLSLASLRRQMGIVTQETLLFNDTIANNIAYGLTDVPRERIEEAARIANA
                     HQFIAEFERGYDTPVGNRGVMLSGGQRQRVAIARALLRDPAILILDEATSALDTESEL
                     LVQEAIDRLMRDRTVLVIAHRLSTVKNADRIIVLDRGRIVECGSHSDLLRHNGLYNRL
                     YTMQFQDVDAHTPA"
     gene            complement(3588..>3971)
                     /locus_tag="PK112_02150"
     CDS             complement(3588..>3971)
                     /locus_tag="PK112_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10883.1"
                     /translation="GSNEYVYEAVPTLLESDACAQAEVRVPLAGLLSTTPDSLGAAED
                     LEVEADYVDPDEPRFVIRGRGCPWRLEIRYDRRDDRWQVKEFRFADGADLWLTAQRRT
                     VKHGAKMSAGRFAVAIPENAVRIVP"
BASE COUNT          711 a         1354 c         1283 g          623 t
ORIGIN      
        1 attcaagaat ctgtccccgg ccggagcggt tgaagtcgta gtaccgggcg tagcgctcct
       61 tgcccacgcc gatccgaggc ttgccgcgcg ccacgtactc cgagaggtac agggttgtga
      121 ccgaatcgcc gtagacgagg tcgaccacgc agtcgtactg cgcccgccgg aggcgccgga
      181 gttcgcggag gtcgtgcggc gcccgcttgg tgtacatgta gatctcgtcg aaacgcgggt
      241 cgcccagggt gatggccgaa tttcgcggcg atgccagcac cccgatgcgc aggtggggat
      301 accgggaccg aagagcgtcg aagacgggga aggaaatgac catgtcgccg agtttgtccg
      361 ggcgcaggaa gagcactttc cgcagcgccg cgccgtcgag cggtccctcc ccgaccggcc
      421 gcggccggag cagcgacgcg gtggcccggt agaagaaccg cttgaacgag tactcgaact
      481 ccttaagccg tcccatggct ctgcccggcc tcctccagtt gcgacaggaa gaaccgctcg
      541 acgtcatcga caagccggtc ggcgctgaac cgctgctcga atcgctcccg cccggcccgt
      601 cccaggcgcg tgcgggcgtc ggaatcgcag gccagggcga ccgccgccgc cgccagggcc
      661 gccggatcgc gcggcgggaa caagactgcg ctcgacccct ccgcaaccac ctcggggatt
      721 cccccgaccc gcgtcgcggc caccggcagc ccggcccgca tcgcttcgat caggacgaac
      781 ccgaagggct cctccaccgc cgggtggagc atgaaatcgc tccccgccag ttccaggtcg
      841 aagtcgcgca gcatacccgc aaaaacgaaa aagctctcca ggcctgcatc cgcgatggcc
      901 gctttgagac gagtctcgag ggggccgtcc ccaagccaga gaaaccgaat gtccggcgcc
      961 tgccggatga tctcgccggc ggccgccacc aggtaggcgt gccccttctg atcgacaaat
     1021 ctcccggagg tcaccgccac cagcgccgcc ggcggcaggc cgtacttctc gcgcagccgc
     1081 cgcgccgcct ggggccgctg ttcgtgcacc ggccggtcgg ggattccgtt cggaatcacc
     1141 gtgaccatct ccggactgat gtagccgtgg gcgacgatct gcttcttgag cgcggccgag
     1201 ggggtgatga cggcgtcgac caggcggggc gtgaggaggc ggtgccggag gcggtctccg
     1261 gcgatgtcca gtccgacccg ccagatcacg cgcgggcggc ccgcccagcg ggcggcgatt
     1321 ccggccagcc ggatgtcttt attgaaattg acggtgacga tatcgacagc gcgctggcgc
     1381 atgaaggtgc gcaggcgcgc gatcacgagc ggaccgaaat ccccggcgat tgccaggtgg
     1441 agcgtctcta cgccccgggt atcgagctcg acccgccgaa ggaactctgc gcccgtccgc
     1501 ccgaccaccg tgacccggtg tccccgcgcg gccaatccgc cggagaccag ccgaatccat
     1561 tcttccatac cgccgtaggt ttccggttcg atggagtcaa gctggagtat gtgcatcgac
     1621 gtcctgaaac tgcatagtgt acagccggtt gtacagtccg ttgtgccgca gcaggtcgga
     1681 gtggctcccg cattcgacaa tgcggccgcg gtcgagcacg atgatgcggt cggcattctt
     1741 gaccgtcgac agccggtggg cgatcaccag caccgtccgg tcccgcatga gccggtcgat
     1801 cgcctcctga acgagcagct ccgactcggt gtcgagggcc gacgtggctt catcgaggat
     1861 cagaatcgcc ggatcgcgca ggagcgcgcg ggcgatcgcc acccgctgtc gctggcctcc
     1921 cgacagcatc accccccgat tccccaccgg cgtgtcgtag ccccgctcga attcagcgat
     1981 aaactggtgg gcgttggcga tccgcgccgc ctcctcgatc cgctcgcgcg ggacgtccgt
     2041 caagccgtac gcgatgttgt tggcgatcgt gtcattgaac aggagcgtct cctgggtgac
     2101 aatccccatc tgccgccgca gcgaggccag cgagagcgtg cggatgtcat gcccgtcgat
     2161 cgccaccctc ccccgctgcg gatcgtagaa gcgcgggagc aggtcgagca gggtggactt
     2221 gcccgcgccc gaaggtccaa cgatcgcgac cacctctccc ttgcgcacct cgaacgtcac
     2281 gtcgcggagg acgtcgtcgg aggtttcgta gcggaaccag acgccctcgt agcggattac
     2341 ctcgcggaac tccggcagca caaccggcgc cgccggttcg cccaccgtct cgggggcgtc
     2401 gagcacctcg aaaatgcgtt ccgccgccgc catcccctcc tgcagcttga tgtggatctg
     2461 gctgagcgat ttgaccggtt tgatcatggc gagcatggcg acgatgaagg tgatgaagtc
     2521 cccggcgctc agctctcccg tcccggcgat gatgcgcgtc cccgccacga gcaggataaa
     2581 cagcccggcc gtcgtcgcca gcatgtcgtt gatcggcgcc gcgaggtgcc ggatccgggt
     2641 catgcgcaga agcgaccgga aatactggtg ggtggcccgg aagaaccgcc cggtctcgaa
     2701 ctcctcggtc gaaaacgcct tcacgatccg caggttggtg accgcctcct ccagcaccga
     2761 gttgacgtcc gccatccgct cctgcgtgcg ggccgagtac ttccgaagct tcttccccac
     2821 gaaccagatg aacacgaaca cgagcggcat cacgatcatc gacagcagcg tcagcttcca
     2881 gctcaggatc acgaggaaag cgaacagcac aatcaccgtg atggcgtcgg tgaccaaatt
     2941 gttgaatccg atgtcgacgg aatcattgag gacgaggacg tcgttggtga tccgggagat
     3001 gacctggccg gtgcgccggc cgtgaaaata cccgagcggc agccgttggt atttctcgaa
     3061 caactggtcg cgaaaacgcc gcaccagggc ttgctggacg taggccatga agtagccccg
     3121 caggtagagg aagacgttct tggcgatcac gaccagaaag gccagccagc agaagttgag
     3181 aagggtctgg cggcggttgt ccgagcgcac gataccgtcg acccaggcct gcatcgactc
     3241 cttgactttg cccagccggg aggagagctt ctggatcatc cccgagttcc cggtggcttc
     3301 gccgggggtc gccggccgga cctcggccgt agccgtcggc gccgccaccg tcgccatcgg
     3361 atcggacacc tcaaacagca ccatgagaag cgggccgagc atccacacca gcaggccggc
     3421 gagcactgcc gagagggccg ccgacaccga ggcggtgacc aggtgcttcc agtagggttt
     3481 cagggccgcg agcgcccgac gatacagatt cactgacttg ctccgtaaga aagtgaccgg
     3541 tcggcaatat cgctccggcg gggagggaag tcaaccgccc ggcggtttca gggtacgatc
     3601 cggacggcgt tttcagggat ggcgaccgcg aaccggccgg cgctcatttt cgccccgtgc
     3661 tttaccgtcc gccgctgcgc cgtcagccac aggtcggcgc cgtccgcgaa acggaactcc
     3721 ttgacctgcc accggtcgtc gcgccggtcg tagcggatct ccagccgcca gggacatccc
     3781 cgcccgcgaa tcacaaagcg cggttcatcg ggatccacgt aatcggcctc cacctcgaga
     3841 tcctccgccg ccccgagcga atcgggcgtg gtcgaaagca gtccggcaag cggcacccgg
     3901 acctccgcct gcgcgcaggc gtccgactcc aggagcgtcg gcaccgcctc gtagacatac
     3961 tcgttggaac c
//
LOCUS       DAOOKS010000085         3963 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_27527_length_3963_cov_3.535312, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000085 DAOOKS010000000
VERSION     DAOOKS010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3963
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27527_length_3963_cov_3.535312"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="PK112_02155"
     CDS             <1..510
                     /locus_tag="PK112_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
                     /protein_id="HPC10884.1"
                     /translation="LTTSGCRTDYFAVRGFIDLNNSLKQGTVQLVSVPSGSSPLPPGS
                     GPVVRLWFTTAGAASGDSAVVSFNGYGSFTPRFVSSIAAYAPKTVSPVLVYQNCCVGT
                     RGDVDNSGSLNVTDLTFLISYLFRGGPEPACYEAGDVDLSGIVAVADVTYLIAHLFRG
                     GSAPYPCSS"
     gene            complement(561..1016)
                     /locus_tag="PK112_02160"
     CDS             complement(561..1016)
                     /locus_tag="PK112_02160"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX pyrophosphatase"
                     /protein_id="HPC10885.1"
                     /translation="MRRPIQVLIFVYRQTARGREYLMLRRIPELRGFWQPISGGVEDD
                     ENLLTAARRELREETGFQTAAVESINYAYRIPAFHDRKLGAAPGADETMPLHTFAARV
                     DDGAEPVLDPAEHDRYLWCPLLLADRLLYWPGDREALRQVDTWLRSRPL"
     gene            complement(1022..1600)
                     /gene="thpR"
                     /locus_tag="PK112_02165"
     CDS             complement(1022..1600)
                     /gene="thpR"
                     /locus_tag="PK112_02165"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HPC10886.1"
                     /translation="MRLFIALPPAEAVAEELGRVIVLLRAKGGPVRWVAPENIHLTVR
                     FLGETDEARVPKLRSLIDSIAPAFAPADVLIDRLGGFPNLRKPRVIWAGLAAAEPVER
                     MAQMARQVELAVRQLRFEPEKKGFQPHLTLGRVKDPRGMEQLAAFMESYRFTPLAVRL
                     DRLVLFRSTLTPKGSIYERVHEAKLGDERLGG"
     gene            complement(1600..2847)
                     /locus_tag="PK112_02170"
     CDS             complement(1600..2847)
                     /locus_tag="PK112_02170"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HPC10887.1"
                     /translation="MPMCAEIITVGDELITGHRIDTNSAFIAQQLTRIGLEVRYRSSV
                     GDSLEMMEEVFRLALKRAQVVIVTGGLGPTDDDITKKAIVRVFKRNLIFHEELLEELK
                     KRYAARGIEMPAINQNQALLPQGATVFPNKYGSAVGIGIAEGGTVFLALPGVPYEMKQ
                     IMTDEIIPYLQRLNIGQVLEVVSLRTTGAVESKLAELIAPGLKLEPGVKLAYLPAPSA
                     IELRVLAAAATREEAQEKAQAVVRYLESTVGKYIFGRGDDTLEGVVGQLLVDNDKSLA
                     VAESCTGGELGMLITSVPGASRYFLGGVVAYSNEAKTELLGVDRDIIAQFGAVSRECA
                     EAMAEGCRRRFGADYALAVTGIAGPGGATPDKPVGTVWIGLASLHETYAKTFSLGTLR
                     ESIRDRAAYTALEMLRRNILDIT"
     gene            complement(2866..3330)
                     /locus_tag="PK112_02175"
     CDS             complement(2866..3330)
                     /locus_tag="PK112_02175"
                     /inference="COORDINATES: protein motif:HMM:NF016489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HPC10888.1"
                     /translation="MKRGLVQLIAGGLYSGASPIVPGTTGSIPPWLIAWFLLAGNQAA
                     LGIVAVLTVFVSVWAAGEAERFYGHDAKKIVIDEWAGMFLTLLFVPYSLVNYLIGFAA
                     FRAFDVVKIPPAAQAERLPRGWGVTMDDVVAGVQANILTQIVVRLIARSAGA"
     gene            complement(3341..>3963)
                     /gene="pgsA"
                     /locus_tag="PK112_02180"
     CDS             complement(3341..>3963)
                     /gene="pgsA"
                     /locus_tag="PK112_02180"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HPC10889.1"
                     /translation="HPASPGLPLLQMNMPNRLTLLRVIIAPIFMYFFLLDSYWMKLTA
                     LVLFVVAALTDFADGYYARKYGIITGFGKFMDPLADKVLVSSALITFIALDVVSPLPV
                     ILIVGREFLITGLRMLAAYRGVVIPPTWWAKVKTFLQLSVVGLVLAYINLILTLEHFD
                     SPARPYFQFDYPFYFNILLWITAAVTVWTGIDYIRKYYYMIKTVLK"
BASE COUNT          685 a         1347 c         1275 g          656 t
ORIGIN      
        1 ctgaccacct cgggctgccg gaccgactac ttcgccgtcc gcgggttcat cgacctcaac
       61 aattcgctca agcagggaac cgttcagctc gtgagcgtcc cctccggttc ctcgccgctt
      121 ccgcccggca gcggcccggt agtgagactc tggttcacca cggccggggc ggcctcgggc
      181 gactcggcgg tcgtctcgtt caacggctac ggctcgttca ccccccgctt cgtcagcagc
      241 atcgccgcct acgcgcccaa gaccgtctcg ccggtcctcg tctaccagaa ttgctgcgtc
      301 gggacccgcg gcgacgtcga caacagcggc tccctcaacg tcaccgatct caccttcctg
      361 atctcgtatc tgttccgcgg ggggcccgag ccggcgtgtt acgaagcggg cgatgtcgat
      421 ctgtccggga tcgtggcggt cgccgatgtg acttacctga ttgcccacct gttccgcggc
      481 gggtccgcgc cgtacccgtg ctcatcgtga ggtgggcgcc gccggtcccg tgtctgcgcg
      541 cggcgggtgc tgaagccggc ctagagcggc cgggaccgaa gccaggtgtc cacctggcgc
      601 agcgcttcgc ggtcccccgg ccagtagagc agccgatcag ccagaaggag cgggcaccac
      661 aggtagcggt cgtgctcggc gggatcgagg accggctccg ctccatcgtc gacccgggcc
      721 gcgaaggtgt gcagcggcat ggtctcatcg gcgcccggag cggcgccgag cttgcggtcg
      781 tggaaggccg ggatgcggta ggcgtagttg attgattcga cggcggccgt ctgaaacccg
      841 gtctcttcgc gcaactcgcg gcgggccgcg gtcaggagat tctcatcgtc ctccactccg
      901 cccgaaatcg gctgccagaa cccgcggagc tcgggaatgc ggcggagcat gagatattcc
      961 cgtccccggg cggtctggcg atagacgaaa atgaggactt gaatgggccg gcgcacggtg
     1021 ctcagccccc cagccgctcg tcgcccagct tcgcctcatg cacgcgctcg tagatcgacc
     1081 ctttcggagt cagcgtcgac cggaacagca cgagacggtc gaggcgcacg gcgagcggcg
     1141 tgaagcggta cgattccatg aaggccgcca gttgctccat cccgcgcggg tctttgaccc
     1201 ggccgagcgt caggtgcggc tggaagcctt tcttttccgg ctcgaaacgc agttgccgca
     1261 ctgcgagctc cacctgccgc gccatctgcg ccatccgctc gaccggctcg gcggcggcca
     1321 gtccggccca gatcacgcgc ggcttgcgga gattgggaaa accgccgaga cggtcgatca
     1381 ggacgtcggc cggggcgaag gccggcgcga tgctgtcgat cagactccgc agcttcggca
     1441 cccgcgcctc gtccgtctcg ccgagaaacc gcacggttaa atggatgttc tccggcgcca
     1501 cccagcggac cggcccccct tttgcgcgca ggagcacgat cacgcggccg agttcctccg
     1561 cgaccgcctc cgccggcggc aaagcgataa acaaccgcat caggtaatat ccaggatgtt
     1621 gcgccgcagc atctccagcg ccgtgtaggc cgcccggtcg cggatgctct cccgcagcgt
     1681 gcccaggctg aacgttttcg cgtaggtttc gtggagggag gcgaggccga tccagacggt
     1741 ccccacgggt ttgtccggcg tggcgccccc gggcccggcg atgcctgtga ccgccagggc
     1801 gtagtcggcg ccgaaacgcc ggcggcaccc ctcggccatc gcctccgcgc actcccggct
     1861 caccgcgccg aactgcgcga tgatgtcccg gtccactccg agcagttccg tcttggcctc
     1921 gttggagtag gccaccacgc cgccgaggaa gtaacgcgag gcgccgggga ccgaggtgat
     1981 gagcatgccg agctcgccgc cggtgcagga ttccgccacg gcgagggact tgtcgttgtc
     2041 gaccaggagc tgtcccacca ctccctcgag ggtgtcgtcg ccgcggccga aaatgtactt
     2101 ccccaccgtc gattcgagat accggaccac cgcctgcgcc ttctcctgcg cctcctcgcg
     2161 ggtagcggcg gccgccagga cgcgcagttc gatggccgac ggggcgggca ggtaggcgag
     2221 tttcaccccc ggctccagct tcaaccccgg ggcgatcaac tccgcgagct tggactcgac
     2281 cgctcccgtc gtccgcagac tcacaacctc gagcacctgc ccgatgttga ggcgctggag
     2341 gtacgggatg atctcatcgg tcatgatctg cttcatttcg tacgggacgc ccggcagggc
     2401 caggaacacc gtcccgccct cggcgattcc gattccgacc gccgagccgt acttgttcgg
     2461 gaaaacggtc gccccctgcg ggaggagcgc ctggttctgg ttgatggcgg gcatctcgat
     2521 accgcgcgcc gcgtagcgct tcttcagctc ttcgagcagc tcctcatgga aaatgaggtt
     2581 gcgtttgaag acgcggacaa tggccttctt ggtgatgtcg tcgtcggtcg gcccgagccc
     2641 gccggtgaca atcacgacct gggcccgttt gagggccagg cggaacacct cctccatcat
     2701 ttcgagcgaa tcgccgaccg agcttcggta ccggacctcg agcccgatcc gggtcaactg
     2761 ctgggcgatg aacgccgagt tggtgtcgat ccggtggccg gtgatgagct cgtcgccgac
     2821 cgtgatgatt tccgcgcaca tgggcatcgc cgccatcctc cggcctcagg cgcccgcaga
     2881 gcgggcgatg aggcggacca caatctgggt caggatgttc gcctgcacgc cggccacgac
     2941 gtcatccatg gtcactcccc acccgcgcgg gagccgctcg gcctgcgccg ccggcgggat
     3001 cttgaccacg tcgaaagccc ggaacgccgc aaacccgatg aggtagttca ccagcgagta
     3061 cggcacgaac agcagggtca ggaacatgcc cgcccattcg tcgatcacaa tcttcttcgc
     3121 atcgtggccg tagaagcgct ccgcctcccc ggcggcccag acggagacga agacggtcag
     3181 gacggccaca atcccgagcg ccgcctggtt gcccgccagg agaaaccagg cgatgagcca
     3241 ggggggaatc gacccggtgg tgccggggac aatcggcgag gcgccgctgt acagcccgcc
     3301 ggcgatgagt tggaccaggc cgcgcttcat tcgccgcccc tcacttgagc accgtcttga
     3361 tcatgtagta gtatttcctg atatagtcga ttcccgtcca gaccgtcacc gcggccgtaa
     3421 tccacaggag gatattaaaa taaaaggggt aatcaaactg aaaatacggc cgcgccggac
     3481 tgtcaaagtg ctcgagcgtg agaatcaggt tgatgtacgc cagcaccagc ccgaccaccg
     3541 agagctgcag aaaagtcttc accttcgccc accaggtcgg agggatgacc accccccggt
     3601 aggccgccag catgcgcagc ccggtgatga ggaactcgcg ccccacgatg aggatcaccg
     3661 gcagcggcga gaccacgtcg agcgcgataa acgtaatcag cgcgctggaa acgagcacct
     3721 tgtccgccag cggatccatg aacttcccga aacccgtaat gatgccgtac ttccgggcgt
     3781 agtagccatc ggcgaaatcg gtcagcgccg ccaccacgaa caacacgagc gccgtgagtt
     3841 tcatccagta gctgtcgagc aggaagaagt acatgaagat cggggcgatg atgacccgaa
     3901 ggagcgtcag gcggttgggc atgttcattt gcagcaaagg taggccgggg gatgcggggt
     3961 gtc
//
LOCUS       DAOOKS010000086         3962 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_27537_length_3962_cov_3.213463, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000086 DAOOKS010000000
VERSION     DAOOKS010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3962
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27537_length_3962_cov_3.213463"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1038)
                     /locus_tag="PK112_02185"
     CDS             complement(<1..1038)
                     /locus_tag="PK112_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HPC10890.1"
                     /translation="MMRTRRPYRLAAMAAAALALGPAAPGAFGQAAGAAPGDTVGSLP
                     GVEVRTSVDRAKIFVGDQVTYTLTIIHDSTIDLVPPPLGANLGAFQVLDYETDRVTRL
                     PDGRVQNESRFVISTFTTGEYAVPPLPVMAIMPDSSRKVLLAEAVPITVESILAAGDT
                     LMDIKPLAGVLGPEAFAKDRTWWYVGGGAAAAALLAALLWALLRRKRKPAAAAVDLRP
                     PWEIASERLALLLHKNLIADGRFKEYYYELSDTLRWYLGRVYDRNVLDWTTEEFLTAF
                     REIELPHSLYDGVEAFMSHADLVKFAKYEPSIEQTKYDFEFVHAAVDRVRQDVFRRQQ
                     GQAAAGGGRPAQ"
     gene            complement(1043..1951)
                     /locus_tag="PK112_02190"
     CDS             complement(1043..1951)
                     /locus_tag="PK112_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10891.1"
                     /translation="MAGRRATALMLGLAWAAAIAGCGGSTAHEAAIERHKKLAAELRD
                     NRLYDAAVEEYRQILAYDDLDDGTRAGVSYLIGRLYFENIGDYEQAAAYYVRARALDP
                     DGDFVLEASKNLVTALERSGNLVNAKRQLDVLTDIGAAPAGDSDVAVARMGGVPVWQS
                     QVDDAMEMLPPEAQKEYTRDREARVRFMRQYVAAELLYHAAIREGFDKDAEIARRTRE
                     LNRQLLVEKYVREKVAPQIQIDTLDLRNFYLANKDSRYGGAPFDSVQARVLLDYQSQK
                     AESAFQDYLALLAQKERVEFLDRNVK"
     gene            complement(1953..2828)
                     /locus_tag="PK112_02195"
     CDS             complement(1953..2828)
                     /locus_tag="PK112_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HPC10892.1"
                     /translation="MLPSEILKKIRRIEIRTKRLVNDLFSGEYHSTFKGQGMEFEEVR
                     QYEPGDDIRLIDWNVTARTGYPHIKKFREERELSVVLLVDASSSGRFGTRERFKSETA
                     AELGALLAFSAIKNNDKVGLIIFTDQIEKFVPPRKGRGHVLRLIREILFFEPKRTGTD
                     VAGALEYLNRVVRRKSVVFLISDFLSEDFHRPLQVANRRHDLIAVKISDPRESRFDDV
                     GLIELEDAETGEVYVIDTGSKEFRREYAARADEDLGRLEREFRLINLDFINIRTDQPY
                     IKPLIAFFRSRERRL"
     gene            complement(2838..3827)
                     /locus_tag="PK112_02200"
     CDS             complement(2838..3827)
                     /locus_tag="PK112_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013377260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HPC10893.1"
                     /translation="MHSEIQEIQARVEGESAFVRRLTDQMAAVIVGQKYLVERLLIGI
                     LSNGHILIEGVPGLAKTLSVHTLASAIQAKFQRLQFTPDLLPADLIGTMIYNPQTAAF
                     SVKKGPVFANIILADEINRAPAKVQSALLEAMQERQVTIGETTFPLEEPFLVLATQNP
                     IEQEGTYPLPEAQVDRFMLMLKIGYPTPAEEREIMERNTGMGTARVEKVVTPGDILQA
                     RQVVRSIYVDDKVKDYIVNIIFATREPQKYGLGELKDLIAFGASPRATIYLNLAAKAH
                     AFLRGRGYITPEDIKAIGHDVLRHRVLLTYEAEAEEVTSDDVVTKVFDAVEVP"
BASE COUNT          613 a         1475 c         1159 g          715 t
ORIGIN      
        1 ctgcgccggc cggccgcccc ccgccgccgc ctgcccctgc tgccgccgga acacgtcctg
       61 ccgcacccgg tcgaccgccg cgtggacaaa ctcgaaatcg tacttagtct gctcgatcga
      121 cggctcgtac ttggcgaatt tcaccaggtc ggcgtgcgac atgaacgcct ccaccccgtc
      181 gtagagcgag tgcggcagct cgatctcccg aaacgccgtg aggaactcct ccgtcgtcca
      241 gtcgagcacg ttccggtcgt acacccgccc gaggtaccac ctcagggtgt cgctcagttc
      301 gtagtagtac tccttgaagc gcccgtcggc gatcaggttt ttatggagca ggagggcgag
      361 ccgctcggag gcgatctccc acggcggccg gagatcgacc gccgccgccg cgggcttccg
      421 cttgcgccgc aggagcgccc acaggagcgc cgccagcagc gccgccgccg ccgctccccc
      481 gccgacatac caccacgtcc ggtccttcgc gaacgcctcc ggcccgagca cccccgcgag
      541 cggcttgatg tccatcagcg tgtcgcccgc cgccaggatc gactccaccg tgatcggcac
      601 cgcctcggcc agcagcactt tccggctgct gtcgggcatg atcgccatga ccggcagcgg
      661 cggcaccgcg tactccccgg tcgtgaacgt gctgatcacg aaccggctct cgttctgcac
      721 ccgcccgtcg gggagccggg tcacgcggtc ggtctcgtag tcgagcacct ggaacgcccc
      781 gaggttggcc ccgagcggcg gcggcaccag gtcaatcgtg ctgtcgtgga tgatcgtgag
      841 cgtgtaggtc acctggtcgc cgacaaagat cttcgcccgg tccaccgagg tgcgcacctc
      901 cactcccggc agcgacccga ccgtgtcgcc gggcgccgct ccggccgcct ggccgaacgc
      961 ccccggcgcg gccggcccca gcgccagcgc cgccgccgcc atcgccgcga gccggtatgg
     1021 ccgtctcgtc cgcatcatcc tctcacttga cattccggtc caaaaactcc acccgttcct
     1081 tttgggccag cagggcgagg tagtcctgga aggccgactc cgccttctgc gactggtagt
     1141 cgagcaacac ccgcgcctgc accgaatcga acggcgcgcc gccgtagcgg ctgtccttgt
     1201 tcgccaggta gaagttgcgc aggtcgagcg tgtcgatctg aatctggggc gccaccttct
     1261 cccggacata cttttccacc agcaactgcc ggttcagttc ccgcgtccgc cgcgcgatct
     1321 ccgcatcttt gtcgaacccc tcccggatcg ccgcgtggta gagcaactcc gcggccacgt
     1381 actggcgcat gaagcgcacc cgcgcctcgc ggtcgcgcgt gtactctttc tgggcttcgg
     1441 gcgggagcat ttccattgcg tcgtcgacct gcgactgcca cacgggcacg ccccccatcc
     1501 gggccaccgc tacgtcggag tccccggccg gcgccgcccc gatatcggtc agtacatcga
     1561 gctgccgctt ggcgttcacg agattacccg accgctccag cgccgtgacg agatttttcg
     1621 acgcctccag cacgaaatcg ccgtccgggt cgagcgctct ggcgcgcacg tagtaggctg
     1681 ccgcctgctc gtagtccccg atattctcga aatagagccg cccgatgagg taactgacgc
     1741 ccgcccgggt gccgtcgtcg agatcgtcgt aggccagaat ctgccggtac tcctccaccg
     1801 ccgcgtcgta gagccggttg tcgcgcagtt cggcggccag tttcttgtgg cgttcgatcg
     1861 ccgcctcgtg ggcggtcgac cccccgcacc ccgcgatcgc cgcggcccag gccagtccga
     1921 gcatcagcgc tgtcgcccgc cgtcccgcca tcctacagcc tccgctcccg gctccggaaa
     1981 aacgcgatca gcggtttgat atacggctgg tcggtccgga tgttgatgaa atcgagattg
     2041 atcaggcgga actcccgctc caggcggccg agatcctcgt ccgcccgggc ggcgtactcc
     2101 cgccggaact ccttcgaccc cgtgtcgatg acatagacct cccccgtctc ggcgtcttcc
     2161 agctcgatca gtcccacatc gtcgaaccgg ctctcccgcg gatccgagat cttcaccgcg
     2221 atcagatcgt gccgccggtt ggccacctgg agcggcctgt gaaaatcctc cgagaggaaa
     2281 tcggagatga ggaaaaccac cgacttccgt cgcaccaccc ggttgaggta ctcgagcgcc
     2341 cccgccacgt ccgtccccgt ccgcttcggc tcgaaaaaga gaatctcccg gatcagccgg
     2401 aggacgtgcc cccgcccctt gcgcggcggc acgaacttct cgatctggtc ggtgaagatg
     2461 atcaggccga ccttgtcgtt gttcttgatc gccgagaacg ccagcagggc tccgagctcg
     2521 gccgccgtct cgctcttgaa ccgctcgcgc gtcccgaacc ggcccgaaga cgacgcgtcg
     2581 accaacagca ccaccgacag ctcgcgctcc tcgcggaact tcttgatgtg cgggtacccc
     2641 gtccgcgccg tcacgttcca gtcgatcagc cggatgtcgt cccccggctc gtactgccgc
     2701 acctcctcga actccatccc ctgccccttg aacgtcgagt ggtactcccc ggagaacagg
     2761 tcgttcacga gccgcttggt gcggatttcg atccgccgga tcttcttgag tatttcgctc
     2821 ggcagcataa acgaatctta cggcacttcc acagcgtcga aaaccttcgt caccacgtcg
     2881 tccgacgtga cctcctcggc ctccgcctcg taggtcagga ggacccggtg gcgcagcacg
     2941 tcgtggccga tcgccttgat gtcctcgggg gtgatgtacc cgcgcccgcg cagaaaggcg
     3001 tgcgccttgg ccgcgagatt gaggtagatc gtcgcccgcg gcgaggcccc aaacgcgatc
     3061 agatccttca gctcccccag cccgtacttc tgcggctcgc gcgtcgcgaa gatgatgttg
     3121 acgatgtagt cctttacctt gtcgtcgaca tagatcgagc gcaccacctg gcgcgcctgg
     3181 agaatgtccc ccggggtgac caccttctcc acccgcgccg tccccatccc ggtgttccgc
     3241 tccatgatct cccgctcctc cgcgggcgtc gggtacccga tcttcagcat cagcatgaac
     3301 cggtcgacct gcgcctcggg cagggggtag gtgccctcct gctcgatcgg gttctgggtc
     3361 gccagcacga ggaacggctc ctcgagcggg aaagtcgtct ccccgatcgt cacctgccgc
     3421 tcctgcatcg cctcgaggag ggccgactgc acttttgccg gcgcccggtt gatttcgtcg
     3481 gcgagaatga tgttcgcgaa aaccggcccc ttcttgaccg agaacgccgc cgtctgcgga
     3541 ttatagatca tcgtgccgat caggtcggcc ggcagcaggt cgggggtgaa ctgcagccgc
     3601 tggaacttgg cctggatcgc gctcgccagc gtgtgcaccg acagcgtctt ggccagcccc
     3661 ggcacaccct cgataagaat gtgcccgttc gacaggattc cgatcagcag ccgctccacc
     3721 agatacttct gcccgacaat cacggccgcc atctggtcgg tcagccgccg gacgaacgcc
     3781 gattcgcctt ccacccgggc ctgaatttcc tgtatctcgc tgtgcatgca acctccatct
     3841 atacggattc aactcgtcag gtcacctttt gcagcgcctc gcgcacttcg gcctcgcggc
     3901 gcacggtctc ccccggcgcc cccgccaccg gtcggaattt gtcctgccgc gcggtcacga
     3961 tc
//
LOCUS       DAOOKS010000087         3955 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_27604_length_3955_cov_3.208462, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000087 DAOOKS010000000
VERSION     DAOOKS010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3955
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27604_length_3955_cov_3.208462"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1608)
                     /locus_tag="PK112_02205"
     CDS             complement(<1..1608)
                     /locus_tag="PK112_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10894.1"
                     /translation="MSALWKIAIAALLLGAALPARGAQWSLAGEVVDLDGAPVIGANV
                     AVWIGSTPVAAGAADEHGRFALRLKDSTAAPLRLVVSSVGYLRHESTVAGGGLPGRLR
                     IELRPAPVAVEGVTVQPPEAAGAPCRALTRAEVAAAAQTSFVPGNPIDAVKEPRVLRE
                     GSSHSARLRVSGSSPVYTINGIAIGRNPTHYGMFAVVPAAVVERMTVRTLGTGAAHDA
                     PAAIDLALPRPYGRHRDASLSLSAIDATAAWSLGGPHWFTLGAARKSVLDEIVNRLDT
                     DAGRCTVPPTNFQDLVASSGLRLGSAASLAIDQYHVHDALEYRTGGTARNTRGAQTAL
                     ATAEHYIGARLEMLRSGTLLRLQAAVQSTEESYAAASVPDGDEAEPPILDLHASGVTT
                     TIAGEVVLSLGACELTIGDRVRHVMKQRIDLTQQNWNFLPPDAASDLPNPYQPELNRL
                     YGSYHGTRRRTDHAAYASLRWRAGPVHMESGARIGHFGGLARKEWAVFRQAATLDLDD
                     KGALTFFAGTFAEDPAQRVLEAYQALVH"
     gene            1824..2744
                     /locus_tag="PK112_02210"
     CDS             1824..2744
                     /locus_tag="PK112_02210"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPC10895.1"
                     /translation="MGEIAGLATSALWAITSILFAEGGKLIGSFKVNNIRLVFAAVIY
                     ASLLTILTGLPFPQDINSRQLFWLGLSGLVGLVFGDSCGFKALVMIGPRLTTLMYATT
                     PIWTTVVAWIFLDERLNWLDILGIVVTIAGIVWVISERRFKKIVPNDIGESHPDAGSL
                     FTGLLLGVGAALGQAVGLVMAKQGMVHAGGAVEPIEASAVRMGVSLVAIWLISGLRGQ
                     IPATVKAARHLRAMKFTLGGAVFGPFLGVWMSLLAVRYIEAGVASTLNSMSPVMILPL
                     LIIIYRERPSLRAWLGAIVAVIGVAILFLN"
     gene            2755..3732
                     /locus_tag="PK112_02215"
     CDS             2755..3732
                     /locus_tag="PK112_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10896.1"
                     /translation="MMRYLDYDAFRAFLSRRCGSEAERRARSVGVDRAHDSGYRDIRR
                     FFGPEAVDPGRVALAYRETPFRLDDPLAASFARRIETQLRAEGRLHRGPMIAAVAEEH
                     FDSPSPRLVLQPCDYGLFAGACFALDLPNSVFSPAGGTLREYHHARGLRLPAGLGVCG
                     LLVTEEPDRTRRVLVVRRAAHLASLTGSVGPSAAGSVDYGVKWKTLEEMARAAMAAEI
                     AEELGLTPAEYAITLLAYAGELFRGGKPQLFCRIDTSLSAATVADRLDAVVARLARDG
                     RLAEHTDFEFLPLDGAARLSDTDVARLNHEAAMNWRLAEESAASTSPGR"
BASE COUNT          553 a         1444 c         1287 g          671 t
ORIGIN      
        1 gtggaccagc gcctggtagg cctcgaggac gcgctgcgcc ggatcctccg cgaacgtccc
       61 cgcaaagaaa gtcagcgccc ccttgtcgtc caggtcgagc gtggcggcct ggcggaatac
      121 cgcccactct ttccgggcca gccccccgaa gtgcccgatg cgcgcgcccg attccatgtg
      181 gaccggcccc gcccgccagc gcagcgaggc gtaggcggca tggtcggtgc gccgccgcgt
      241 cccgtggtag gatccataga gccggttcaa ttccggctga tagggattgg gcaggtcgct
      301 cgccgcatcc ggggggagga aattccagtt ctgctgcgtc aggtcgatcc gctgcttcat
      361 cacgtgccgg acccggtctc cgatcgtcag ttcgcacgcc ccgagcgaca gcacaacctc
      421 gcccgcgatc gtcgtcgtca ctccggaggc gtgcagatcg aggatcggcg gttcggcctc
      481 gtccccgtcc ggcaccgacg ccgccgcata cgactcctcc gtgctctgga cggccgcctg
      541 caggcggagg agcgtccccg accggagcat ctccaggcgc gccccgatat agtgctccgc
      601 ggtcgccagc gcggtctgcg ccccccgcgt gttgcgcgcg gttccgccgg tgcggtactc
      661 gagcgcatcg tgcacgtggt actggtcgat cgccagcgag gcggcgcttc cgagccggag
      721 ccccgacgaa gcgaccaggt cctgaaaatt ggtcggcggc accgtgcacc gcccggcgtc
      781 ggtgtcgagc cggttgacga tttcgtcgag caccgacttg cgcgccgccc cgagggtaaa
      841 ccagtgcggt ccgccgagcg accaggccgc ggtggcgtcg atcgccgaca ggctcagcga
      901 cgcgtcgcgg tggcgcccgt acggccgggg gagggccagg tcgatcgccg ccggcgcatc
      961 gtgcgccgcg cctgtcccca gcgtgcgcac cgtcatccgc tccaccacgg cggccggaac
     1021 gaccgcgaac atgccgtagt gggtcgggtt tcggccgatg gcgatgccgt tgatcgtgta
     1081 gaccggcgag gagccgctga cccgcagccg cgccgagtgc gacgaacctt cccgcaacac
     1141 ccgcggctcc ttcacggcgt cgatcgggtt gcccggcacg aacgatgttt gggccgccgc
     1201 cgcgacctcg gcccgcgtca gcgcccggca cggcgctccg gctgcctccg gcggctgcac
     1261 cgtcaccccc tccaccgcca ccggcgccgg ccgcagttca atgcggagcc ggcccggcag
     1321 tccgccgcct gcgaccgtgc tctcgtgccg gaggtatccg accgatgaga cgaccaggcg
     1381 cagcggggcc gcggtcgaat ccttcagccg cagagcaaac cgcccgtgct catcggccgc
     1441 tccggccgcc accggcgtgg agccgatcca gaccgcgacg tttgccccga tcaccggcgc
     1501 gccgtcgaga tcgaccacct ctccggccag cgaccactgc gcgccgcggg ccggcaaagc
     1561 ggcccccagc aaaagcgcgg cgatggcgat tttccacagg gctgacatag ggggttctct
     1621 ccgggggact caggacaaac gccgtgcccg gatccggaaa atgcttgccg gttgtatccc
     1681 gttatcttgc agatacttga cgaagtgcgc gggccgccct cggtatgaac ccaggtgctt
     1741 gttatgcacc ggcggacaca ctgccgccga caacagtgtt tgccggagaa accgccctcc
     1801 cctatattcg cccgatgccg tacgtgggtg aaattgccgg tctggccacc tccgccctct
     1861 gggcgatcac ctcgatcttg ttcgccgagg ggggcaagct catcggctcg ttcaaggtca
     1921 acaacatccg gctcgttttc gccgccgtca tctacgcatc gctgctgacc attctcaccg
     1981 gcttgccctt tccccaggat atcaactcac ggcagttgtt ctggctcggt ctttcgggat
     2041 tggtcggcct ggtgttcggc gacagctgcg gcttcaaggc gctcgtgatg atcgggccgc
     2101 gtctgaccac gctcatgtac gccaccactc cgatatggac caccgtcgtc gcgtggatct
     2161 tcctcgacga gcggctcaac tggctcgata tcctcggcat cgtggtcacg atcgcgggga
     2221 ttgtctgggt gatctcggaa cgccggttca agaagattgt cccgaacgac atcggcgaga
     2281 gccaccccga cgccggttcg ctcttcactg gtctgttgct gggcgtcggc gccgctctgg
     2341 ggcaggcggt cgggctagtg atggccaagc aggggatggt gcacgccggc ggcgcggtcg
     2401 agccgatcga ggcctcggcc gtccgcatgg gcgtctcgct cgtcgccatc tggctcatct
     2461 ccggcctgcg cgggcagatc cccgccaccg tgaaggcggc gcgccacctg cgcgccatga
     2521 aattcaccct cggcggcgcg gtcttcggac ccttcctcgg cgtgtggatg tcgctgctgg
     2581 cggtgcgcta catcgaggcg ggcgtcgctt cgacgctcaa ttccatgtcg ccggtcatga
     2641 tcctgccgct cctgatcatc atctaccggg agcggccctc gctgcgcgcc tggctcggcg
     2701 ccatcgtcgc cgtgatcggc gtcgccattc tcttcctgaa ctgagaggaa ccggatgatg
     2761 cggtatctgg actacgatgc gttccgcgct tttctcagcc gccgctgcgg ttccgaggcc
     2821 gaacgacgcg cccgctcggt gggggtcgac cgcgcgcacg actccggtta ccgcgatatc
     2881 cggcgcttct tcggccccga agccgtcgat cccggccggg tcgccctcgc gtaccgggaa
     2941 acaccgttcc ggctggatga tccgctcgcg gcctcgttcg cccgccgcat cgagacccag
     3001 ttgcgcgccg aggggcgtct gcaccggggc ccgatgattg ccgcggtcgc cgaggagcat
     3061 ttcgactccc cctccccccg tcttgtgctt cagccctgcg actacggcct cttcgccggc
     3121 gcctgcttcg cgctcgactt gcccaacagc gttttctccc cagccggcgg cacgctgcgc
     3181 gagtaccatc acgcccgcgg cctccgcctc cccgccgggc tgggcgtctg cgggctgctc
     3241 gtcaccgaag agccggaccg cacccgccgc gtcctggtgg tgcgccgggc cgcccacctc
     3301 gcctcgttga ccgggtcggt aggcccgtcg gccgcgggtt cggtcgacta cggggtgaaa
     3361 tggaaaacgc tggaggagat ggctcgcgct gccatggccg cagagatcgc cgaagaactc
     3421 ggattgacgc cggccgagta cgcgatcacg ctgctggcct acgccggtga gttgttccgc
     3481 ggcgggaaac cgcagctctt ctgccgcatc gacacctccc tgtctgcggc gactgtcgcc
     3541 gaccgcctcg acgctgtcgt cgcccgcctc gcgcgcgacg gccgcctggc cgagcacacc
     3601 gattttgaat ttctcccgct cgacggcgcc gcccgcctct cggacaccga cgtcgcccga
     3661 ctcaatcacg aagcggcgat gaactggcgc ctggcggaag agagcgccgc aagcacatcg
     3721 ccggggcgct agccttcgga cctgggtccg tcacgcggcc tgctgttgat ccggagaaat
     3781 cgcgcctgtg cgaacgggag actcggcctt ggccgccccg tttcggctcg accggcccgc
     3841 cgtcaccggg cagccccggc aaaaaagacc cgcccctggc ggggcgggtc ttcagaatct
     3901 tccggctgtg atcgcagcct acatttccac gtcgtcgaca tcggtggtcg gccgc
//
LOCUS       DAOOKS010000088         3948 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_27667_length_3948_cov_3.190085, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000088 DAOOKS010000000
VERSION     DAOOKS010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3948
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27667_length_3948_cov_3.190085"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="PK112_02220"
     CDS             complement(<1..412)
                     /locus_tag="PK112_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10897.1"
                     /translation="MFRLIAVVMVSALVAAAAPAAGTPGRGSVPTVTVLPGAGDTLPV
                     TPTYEWIDIYCGEPTLDGAPLAPGDTIFVYDPDNMCCGAAIVHADGMYGFAPVYRDDA
                     YSAEDEGAEPGDTLRFVVNGVPVAAAPPVIWTANG"
     gene            complement(667..867)
                     /locus_tag="PK112_02225"
     CDS             complement(667..867)
                     /locus_tag="PK112_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10898.1"
                     /translation="MTPRTVFRCTYIRACYLADELKCFGYKTDCPLYRKCNGEYSDEA
                     AFHEAMDRLIDRTRAVYAGLDK"
     gene            1176..1937
                     /locus_tag="PK112_02230"
     CDS             1176..1937
                     /locus_tag="PK112_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10899.1"
                     /translation="MQSRIARACLLCLAGMVLLAPAAAAQDPGIPDTVHVDSIGAFTN
                     TRAIVPVNFYNDEPLGGLEVTLHFSSPDVHVDSFSFAGGRLQSYSVKGTYTPSGGFSI
                     YCVPFAGEPTIPAASGLLGRLYLSWSVGISPQLVSIDTITIVNVDVSYSTRFSTASAV
                     AFTPKYRRGWVKIDQGFGCCIGTRGNVDGDPTDAVTLPDLTRLIGILFRGMAPADCPE
                     EANINGDATPQPTVADITYLIAYLFRGGPPPAPCQ"
     gene            complement(2009..2464)
                     /gene="fliJ"
                     /locus_tag="PK112_02235"
     CDS             complement(2009..2464)
                     /gene="fliJ"
                     /locus_tag="PK112_02235"
                     /inference="COORDINATES: protein motif:HMM:TIGR02473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HPC10900.1"
                     /translation="MKKFRFRLEALLKQREQIEKDRRRELAEAMRRTARQKQALDGLI
                     ERSRETLAHKRARQTGTLAVAELQLCARFLHRLKRETLGADEVLRALRKTEAKKREAL
                     LEAAKNRKIYDTLKDHRRSEFYHEAEQRERKETDEIGLNVFRAGRGKPA"
     gene            complement(2482..3777)
                     /locus_tag="PK112_02240"
     CDS             complement(2482..3777)
                     /locus_tag="PK112_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018704274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HPC10901.1"
                     /translation="MYAERIRAAHTIRHYGKVMQVVGLVIESAGPAVSIGKLCTIENH
                     DTGAKIRAEVVGFRDNRILLMPLGPITGITPGAIVTSTAEHLRVPVGRELIGRVLGGL
                     GQPIDGKGPLGTGAWRQLSGEPIPALKRRRITEPLRTGIRAVDLVTAVGKGQRLGIFA
                     GSGVGKSIMLGMMARGTSADVNVLALVGERGREVREFVESDLGPEGMRRSVVVAVTSD
                     QPALIRIKGALVATAIAEHFRDQGMDVLLLMDSLTRIAMAQREIGIAIGEPPTAKGYT
                     PSVFALLPGLLERAGQAEKGSITGLYSVLVEGDDFNEPVSDAVRSILDGHVALSRRLA
                     ARNQYPAVDILDSVSRLMKSVCTPEQVELAGQVRELLSVYREAEDLINIGAYVRGSNP
                     KIDRAIAKMDALDAFFRQGIHEISDFDQSVAQLRQLLAD"
BASE COUNT          626 a         1295 c         1278 g          749 t
ORIGIN      
        1 ccccgttcgc cgtccagatc acggggggcg cagccgcaac gggcacgccg ttgaccacaa
       61 agcgcagcgt atcccccggt tccgcgcctt catcctcggc cgaataggcg tcatcccggt
      121 agacgggagc gaacccgtac atgccgtcgg catggacaat tgccgcgccg cagcacatgt
      181 tgtccgggtc gtagacgaag atcgtgtcgc ccggcgccag cggcgcgccg tcgagcgtgg
      241 gctcgccgca gtatatgtca atccactcgt aggtgggcgt cacgggcagg gtgtcgcccg
      301 cccccggcaa gacggtgaca gtcggcacgc tccctcttcc gggcgtgccg gccgccgggg
      361 cggcggccgc gacgagtgcg ctcaccatga cgaccgcaat taaacgaaac attccgagcc
      421 tccacacata ggtcgactgc acaccacgca tctgcttgtt tacgcgagtc gtagatcgcg
      481 gcaggaccct aatgaacccg cgctccaccg ccgccagggc gggggcgcca tgcagtatgg
      541 ggcaaggata ggcagatttc ggcaaatccg caagagggat tgcgcgccga ccgggggcgc
      601 ggggctcccc gaattcgtca ggacagatcc tggcagcggc gctctccccg gtcacgggga
      661 agggtcttac ttgtccagac cggcgtagac cgcccgggtc ctgtcgatca ggcggtccat
      721 cgcctcgtgg aatgccgcct cgtcggagta ttcgccgttg cacttccggt acagcgggca
      781 atcggtcttg tacccgaagc acttaagctc atccgcgagg tagcaggccc ggatgtaagt
      841 acaccggaat actgtcctgg gggtcatttc ctccggcata tcccctgtat cggcgggcgg
      901 ggtgaggttt gtgagacgct ccccgtattc cggggtggga aaagggttgc agcaccgctt
      961 gtgcaacccg cccggccgcg gttggcggac gcctgatctt tccggttgcg ccgccggtcg
     1021 attgtcccta tattactttc cggaaagagc ttcgctcata gttacttaca ttcacccccc
     1081 ggttccggcc gggcaacctg gtaggccaag cgacctttct tcgataacgc gcattgaatt
     1141 tgctcagttc agtcgtgaaa gaaagggtta tccgtatgca gagtcggatt gcacgagctt
     1201 gcctgttgtg tcttgccggg atggttctcc tggcgcccgc cgcggcggcg caggatcccg
     1261 gtattccgga cacggtgcac gttgactcga tcggcgcttt caccaacact cgcgctatcg
     1321 ttccggtcaa cttctacaac gacgagccgc tgggcgggct ggaggtgacg ctgcacttca
     1381 gttctcccga tgtccatgtc gactccttct cgttcgcggg cggccggctg caatcgtatt
     1441 cggtcaaggg gacctacacg ccttccgggg gcttctcgat ctactgcgtg ccgttcgcgg
     1501 gagaacccac gatcccggcc gcttccggcc tcctcgggcg gctctatctc tcgtggagcg
     1561 tgggaatcag cccgcagctg gtgagcatcg acaccatcac gattgtgaat gtcgacgtct
     1621 cctattcgac caggttctcg accgcgtcgg ccgtggcctt cacgccgaaa taccggcgcg
     1681 gctgggtgaa gattgaccag gggttcggct gctgcatcgg gacccgggga aatgtcgatg
     1741 gcgatccgac cgacgccgtg acgctgccgg atctcacccg cttgatcggg attttgttcc
     1801 gcggcatggc cccggctgat tgtccggaag aagccaacat caatggcgac gccacgcctc
     1861 agccgacggt agccgacatc acgtacctca tcgcctacct cttccggggc ggtccaccgc
     1921 cggctccgtg ccagtagcgg ccggagttga tggtcgcagg ccattcaaaa tgattctgaa
     1981 gacagcccgg cgccgccggg ctgtttgttt aggccggttt tccgcggccc gcccgaaaga
     2041 cgttcagccc gatttcgtcg gtctctttcc gctcgcgttg ttcggcctcg tggtagaatt
     2101 cgctgcgtcg gtgatccttg agcgtgtcgt agatcttgcg gttcttggcg gcctcgagaa
     2161 gcgcctcgcg tttcttggcc tcggtcttgc ggagggcgcg gaggacctcg tccgccccca
     2221 gcgtctcgcg tttgagccga tggaggaagc gcgcgcataa ctgcagttcg gcgacggcca
     2281 gggtgcccgt ctgccgcgcg cgcttgtgtg cgagcgtttc ccggctgcgc tcgatgaggc
     2341 cgtcgagcgc ctgtttctgc cgcgccgtcc ggcgcatcgc ctcggcgagt tcgcgccggc
     2401 ggtccttctc aatctgttcc cgctgcttga ggagcgcttc caggcgaaaa cgaaacttct
     2461 tcacgcggcg cctccgggac ctcagtcggc gaggagctgc cggagctggg cgaccgattg
     2521 gtcgaagtcc gagatttcgt gaataccctg ccggaagaac gcgtcgagcg catccatctt
     2581 ggcgatggcc cgatcgatct tgggattgga acccctcacg taggccccga tgttgatgag
     2641 gtcctcggcc tcgcggtaca ccgagaggag ctcccgcacc tggccggcga gttcgacctg
     2701 ctcgggcgtg cagaccgatt tcatgagccg gctgacggaa tcgaggatgt cgacggcggg
     2761 gtactggttg cgggcggcca ggcggcgcga cagagccacg tgcccgtcga ggatcgaccg
     2821 cacggcgtcg gaaaccggct cgttgaagtc gtcgccctcg accagcacgc tgtacaggcc
     2881 ggtgatcgat cccttctcgg cctggccggc ccgctcgagc aggcccggca ggagggcgaa
     2941 gaccgagggc gtgtatcctt tggcagtggg cggctcgccg atcgcgatgc cgatctcccg
     3001 ctgcgccatc gcgatacggg tgagcgagtc catcaagagg agaacgtcca tcccctgatc
     3061 gcggaagtgt tcggcaatgg cggtcgccac cagagccccc ttgatgcgga tgagagccgg
     3121 ttgatcggag gtgacggcca ccacgaccga gcgccgcatc ccctccgggc cgaggtcgga
     3181 ttcgacaaac tcgcgcactt cgcggccgcg ttcgccgacg agggcgagga cgttgacatc
     3241 ggcgctcgtc ccccgcgcca tcatcccgag catgattgat ttgcccacgc cggagccggc
     3301 gaaaatcccc aggcgctgtc cctttccgac agcggtcacg aggtcgacgg cccggattcc
     3361 ggtgcggagc ggttcggtga tccgccgccg tttcagcgcc gggatcggtt cgccgctgag
     3421 ttgccgccac gcgcccgtgc cgagcggacc cttgccgtcg atcggctgcc ccaggccgcc
     3481 gagcacgcgc ccgatcagtt cgcggccgac cggcacccgc aggtgctcgg cggtcgaggt
     3541 gacgatcgcc ccgggcgtga tgcccgtgat cggcccgagc ggcatcagca ggatccggtt
     3601 gtcccgaaaa cccactactt ccgcccggat cttggctccg gtgtcgtggt tttcgatggt
     3661 gcagagcttt ccgatcgaga ccgccggccc ggccgactca atcacgagac cgaccacctg
     3721 catcactttg ccgtagtgtc ggatggtgtg ggcggcccga atccgctcgg cgtacatatg
     3781 ataggaggga gcgctcactg gccggcctct tcggcgagga gaacctcgcg cacgacttcg
     3841 aactgcgatt ccagccgggc gtcgacatcg ccgtgcggcg tttcaatcaa acatccccct
     3901 tcgcgaacgc gggggtcggg ctcgatcgtg agttccttga tcagggcc
//
LOCUS       DAOOKS010000089         3937 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_27783_length_3937_cov_2.931994, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000089 DAOOKS010000000
VERSION     DAOOKS010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3937
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27783_length_3937_cov_2.931994"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3081
                     /locus_tag="PK112_02245"
     CDS             <1..3081
                     /locus_tag="PK112_02245"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPC10902.1"
                     /translation="EQTATEVEAVRVVGQKDKLEIKEVSNQVTITKEEIRTQPVTTVD
                     ELLTQVAGVVTNSQGEVFIRGGRAGEVSYIVDGVPLDDPLGASGAQMSLVSGSIQEFT
                     VIKDGFDPEYGDALSGIVKLTTQTGSRDKTNFDFQYITDDFGNKDLNKYSRNNDYLRM
                     ALSGPDPFVKNKILPALGLRFLEDQEFTYYFYAEMDKNDGIYQYEDYTSPTTARQYGD
                     FNLLGFQIPERLNNRYYYMANLKFRPQQNLRFILSYKESLRRLTLFSWAYRYTPNTAP
                     VYEDQWRTVSLEISGSMSKDMTYEAVFSYYSTYKQQKPGDPKNPGKGLDPDDFPADDE
                     WEYYDDDNGNGQYDPPEPIINLYPDTISYGEGFTGPRYNGGEYGVDFRNQFYDVFELY
                     DQQNSTYWGTGFSFNDNNFADTLEGEPFIDLNGNGVWDQGDYLHDKNGNGVLDNYRVS
                     NVGAAQAEPYVDGDSILGEPYYDNNNNGVYEEGVDGFVMSTGPDNQDYNHNGRRDGPN
                     DKFTGGRVILGPNGDTVRVEPPTPYIDRNGNGIYDGPNFQYDYGEPFTDINGNGKWDA
                     GGTSSFLAPGSYDDDIEWYYRESSTLRGDVKVFRQMGNHELKGGIGMWRYDFTLQDIK
                     RPYVRYNGRPDSGPFPDRGAFRDMYSYQPWAGTVYLRDKLEYGSMIASLGLRWDFFIQ
                     DIDDLIPVAENDDIGSGVILGDRQKLSPRIGFSYPISDKAKVHFNYGHFYQMPDLDAM
                     YARNTTSIQQDAVIGNYNLDYMKTVQYSFGVKYAMSEYYNIDVSGYFKDEFDKINSAF
                     VVIDNFNRQQYRNKDYGRSRGFEVELEKRGGGYVNGMIAYTYGFAYGKASEASRTYQT
                     SFELSREPLDEAPLDNDIRHSLKGNIQVTVPNTVKPRLFGMPIPNGWSLNLQSIIESG
                     RPFTPDRSYPNVATVGGEDIQRNSLRMPSTVLFDVRFTKDFQFAGVDLSYILWIENIF
                     DTRNVAEDGIYTNTGRADTRQNQSGIIRGGTEYDANPYNWDYGRQVRMGIQINL"
     gene            3126..>3937
                     /locus_tag="PK112_02250"
     CDS             3126..>3937
                     /locus_tag="PK112_02250"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HPC10903.1"
                     /translation="MLRHEHTRALGRTRGIALSGAVLAAVLLLVSPGDVRAQAKVGTT
                     GAQFLEIGVSARAMGMAEAFTALANDVSAVYYNPAGLVYLYGPEGQLTYVRMPADVNY
                     GYAAIGLPLESIGGVLGISAYHLNSGEIDEIDYYAIPTGRTFWWKDFSLAVSYGRYLT
                     DRFSVGFTVKYIGEFVHDYSANGWAADVGTVYNTGFRNFKIAMAITNFGPDLNMIRDD
                     FPLPINFRFGGTIDALQTDNYVLSVAAEGCHPSDNLEKYNSGAELTIRDMFIL"
BASE COUNT          833 a         1257 c         1190 g          657 t
ORIGIN      
        1 gagcagacgg cgaccgaggt ggaggcggtg cgtgtcgtcg gccagaaaga caagctggaa
       61 atcaaagagg tctccaacca ggtcaccatc accaaggaag agatccgcac gcagccggtg
      121 acgacggtcg acgagctgct gacgcaggtg gcgggcgtgg tcaccaatag ccagggtgag
      181 gtgttcatcc gcggcggccg cgccggcgag gtgtcgtaca ttgtcgacgg cgtgccgctg
      241 gacgatccgc tcggggcctc gggcgcgcag atgtcgctcg tgtcgggctc catccaggag
      301 ttcaccgtca tcaaagacgg gttcgacccg gagtacggcg atgcgctctc gggcatcgtg
      361 aaactcacca cccagacggg ctcgcgcgac aaaaccaatt tcgacttcca gtacatcacc
      421 gacgatttcg ggaacaagga cctcaacaag tactcgcgca acaatgacta cctgcgcatg
      481 gcgctctccg gtcccgatcc cttcgtcaag aacaagattc tcccggcgct cggcctgcgc
      541 ttcctcgagg accaggagtt cacgtactac ttctatgccg agatggacaa gaacgacggc
      601 atctaccagt acgaggacta cacctcgccg accacggccc ggcaatacgg ggatttcaac
      661 ctcctcgggt tccagattcc ggagcggctg aacaaccggt actactacat ggcgaacctg
      721 aagttccggc cgcagcagaa cctccgcttc atcctctcct acaaggagtc gctgcgccgc
      781 ctcacactct tcagctgggc ctaccggtac acgcccaaca cggcgccggt gtatgaggac
      841 cagtggcgca cggtctcgct ggagatctcg ggatcgatgt ccaaggacat gacgtacgag
      901 gcggtcttct cgtactactc gacctacaag cagcagaagc cgggcgaccc gaagaacccc
      961 ggcaagggtc ttgaccccga cgatttcccg gccgatgacg agtgggagta ctacgacgac
     1021 gacaacggca acggccagta cgatccgccg gagccgatca tcaatctgta ccccgacacg
     1081 atcagctacg gcgagggatt caccgggccg cgctacaacg gcggcgagta cggcgttgat
     1141 ttccggaacc agttttacga cgtctttgag ctgtacgacc agcagaacag cacgtactgg
     1201 ggcaccgggt tttctttcaa cgacaacaat ttcgcggaca cgctcgaggg cgagccgttc
     1261 atcgatctca acggcaacgg cgtgtgggac cagggcgact acctgcacga taagaacggc
     1321 aacggcgtgc tcgacaacta ccgcgtgagc aacgtcggcg ccgcccaggc cgaaccgtat
     1381 gtcgacggcg actcgatcct gggcgagccc tactacgaca ataacaacaa cggcgtgtac
     1441 gaagagggcg tcgacggatt cgtgatgagc accgggccgg acaaccagga ctataaccac
     1501 aacgggcggc gcgacggtcc gaacgacaag ttcaccggcg ggcgcgtcat cctcggaccg
     1561 aacggggaca ccgtgcgcgt cgaacccccg accccgtata tcgaccgcaa cggcaacggc
     1621 atctacgacg ggcccaattt ccagtacgac tacggtgagc cgtttacgga catcaacggc
     1681 aacggcaaat gggacgccgg cgggacctcg agcttcctcg cgccgggatc ctacgacgac
     1741 gacatcgaat ggtactaccg cgagagcagc acactccggg gtgacgtcaa ggtcttccgc
     1801 cagatgggca accacgagtt gaagggcggc atcgggatgt ggcgctacga cttcaccctg
     1861 caggatatca aacggccgta cgtccgctac aacggccgtc ccgacagcgg gccgttcccc
     1921 gaccgcggcg ccttccgcga catgtacagc taccagccct gggccggtac tgtctacctg
     1981 cgcgacaagc tggagtacgg ctcgatgatc gcgtcgctcg gcctgcgctg ggatttcttc
     2041 atccaggaca tcgacgacct gatcccggtg gccgagaacg acgacatcgg atcgggcgtc
     2101 atcctgggcg accggcagaa actctcgccg cgcatcgggt tctcctaccc gatctcggac
     2161 aaggcgaagg tccacttcaa ctacgggcac ttctaccaga tgcccgacct cgacgcgatg
     2221 tacgcgcgca acacgacctc gatccagcag gacgcggtca tcggcaacta caacctcgac
     2281 tacatgaaga ccgtgcagta ctcgttcggc gtcaagtacg ccatgagcga gtactacaac
     2341 atcgacgtct ccggctactt caaagacgag ttcgacaaga tcaacagcgc gttcgtcgtc
     2401 atcgacaact tcaaccgcca gcagtaccgg aacaaggact acggccgcag ccgcgggttc
     2461 gaggtcgagc tggagaagcg gggcggcggt tatgtcaacg ggatgatcgc ctacacctac
     2521 ggctttgcct acggcaaggc gtcggaggcc tcccgaacct accagacgag tttcgagctg
     2581 tcgcgcgagc cgctcgacga ggcgccgctg gacaacgaca tccggcactc gctcaagggg
     2641 aacatccagg tgaccgtgcc caacacggtg aagccgcggc tgttcgggat gccgattccg
     2701 aacggctggt cgctcaatct ccagtcgatc attgagagcg gtcgcccctt cacgccggac
     2761 cgctcctacc cgaacgtggc gactgtgggc ggcgaggaca ttcagcggaa ttcgctccgc
     2821 atgccctcga cggtgctttt cgacgtgcgg ttcaccaagg acttccagtt tgcgggggtt
     2881 gacctgagct acattctgtg gatcgagaat attttcgaca cccggaacgt ggccgaggac
     2941 ggcatttata cgaacacggg ccgcgcggac acccgccaga accagagcgg gatcatccgc
     3001 ggcggcaccg agtacgatgc caacccgtac aactgggact acggccggca ggtccggatg
     3061 ggaattcaga tcaacctgta agtcatggga tgaggcaagt tgttgctgca aataaagagg
     3121 atgccatgct aagacacgaa catacacgcg cgcttggccg gacccggggg atagccctct
     3181 cgggggcggt cctggcggcg gtcctgctgc tggtctctcc cggtgacgta cgggcccagg
     3241 cgaaagtggg cacgaccggc gcccagttcc ttgagatcgg cgtctcggcg cgcgccatgg
     3301 ggatggcgga ggcgttcacg gcgctggcca acgacgtctc ggccgtctac tacaaccccg
     3361 ccggcctggt ctacctgtac gggccggagg ggcagttgac gtacgtgcgg atgcccgccg
     3421 acgtcaacta cggctacgcg gccatcggtc tgccgctcga atcgatcggc ggggtgctgg
     3481 gaatcagcgc ctatcacctc aactccggcg agatcgacga gatcgactac tacgcgatcc
     3541 ccaccgggcg gacgttctgg tggaaggact tttcgctcgc cgtgagctat ggccgctacc
     3601 tgacggaccg gttctcggtc ggtttcaccg tgaagtacat cggtgagttc gttcacgact
     3661 actcggccaa cgggtgggcg gccgacgtcg gcacggtgta caacaccggt ttccggaatt
     3721 tcaagatcgc catggccatc accaacttcg gccccgatct caacatgatc cgggacgact
     3781 tcccgttgcc catcaacttc cggttcggcg ggacgatcga tgcgctgcag accgacaact
     3841 acgtgctgtc ggtggcggcc gagggctgcc acccgtcgga caacctcgag aagtacaaca
     3901 gcggcgccga gctgaccatc cgcgacatgt tcatcct
//
LOCUS       DAOOKS010000090         3928 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_27873_length_3928_cov_3.155435, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000090 DAOOKS010000000
VERSION     DAOOKS010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3928
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27873_length_3928_cov_3.155435"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="PK112_02255"
     CDS             <1..386
                     /locus_tag="PK112_02255"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HPC10904.1"
                     /translation="GVGLGDGRQKHFFLPYPHTDFIFAAAGEEIGLVGLLLLLSLLGF
                     ILYRGFKIAADQPDRFGFLLASGVVLSLFINIAVNVAVVTSLMPVTGLPLPFISYGGS
                     SLLITSITIGVLLNLSRRMVGRAKQ"
     gene            383..1528
                     /gene="murG"
                     /locus_tag="PK112_02260"
     CDS             383..1528
                     /gene="murG"
                     /locus_tag="PK112_02260"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HPC10905.1"
                     /translation="MTGRTQEYRLVFAGGGTGGHLFPAIAIADRVRELLAGTAPVEIL
                     FVGTRRGLEYRMRDALGYPLHVINVRGLVRALSPANLLVPFVLVTALVRAWLLLGRFR
                     PDAVVGTGGYVALPVLKAARWRGVPTVLQEQNSFPGITTRQGAKHARKIYLGFDGARQ
                     YLRTEAEVLTTGNPVRRGLSGGDRAAAAAAFGLDPAKKTILVLGGSQGARAINEAVLR
                     SLAGGAPADGCQILWQTGKRDYTEVSAKAGGTAARCALFPFAHDMAAVYAAADLAVAR
                     AGALTLAELAACAIPAILIPYPHAAGDHQRKNARDLAAAGLARVIDENDLPRHDLIAE
                     AADLLEAAEYRTMREKLESARRQGRPATDIIAEDIIDLINRERKARA"
     gene            1585..2964
                     /gene="murC"
                     /locus_tag="PK112_02265"
     CDS             1585..2964
                     /gene="murC"
                     /locus_tag="PK112_02265"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013581043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HPC10906.1"
                     /translation="MFGKIRKLYFVGIGGSGMSGIAEILHNLGFEISGSDQTASDLTR
                     YLAERGLRIHEGHDAAHLGDAEVVVISSAVGEANPEVVEARRRGIPVIKRAEMLGELM
                     RLKYSIGVAGTHGKTTTTSMIGRILQSGGLKPTIIVGGIVAELGTGASLGEGEYLVAE
                     ADEFDRSFLAMFPTMAVVTNLEADHLDCYRGLDDLGHAFLTYMNRVPFFGSVVLSADN
                     AQLMQMRDRLARPYVTFGFSVEADYRAVEVRLEPGRSVFSLYRRGQYLGDILLRVPGR
                     HNVANALAAAAACTELGVPFELIADGLRGFTGVGRRFEIVGEAGGVTVVDDYAHHPTE
                     VEATLRTARDVYGRRVIVVFQPHLFSRTRDFADEFAQALAIADQCLLVDIYPAREKPM
                     PGVTADLIREKAAARGLGQFTCIGERQNAAREVVQRAQPGDIVITMGAGSITLERKNI
                     LEALRERWG"
     gene            2955..3752
                     /locus_tag="PK112_02270"
     CDS             2955..3752
                     /locus_tag="PK112_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10907.1"
                     /translation="MGISGRKYRLFALAGLLVGLGLTVTVNFTDACRLRAVTVDGKPV
                     ENWRQQFAMLRPGSLAAQPVERLAKKLLADEDVYKVDVRFELPGGIDVRTNEFRPVCF
                     VVGMETGKLFGLNRRARLIALDAPDINWEQPVFTGLTTGALHSICADPRVLPVIDQLE
                     DLRESRADLYRLLDEIDFGEREYLEVTFAGLPYRLRVRPDHVLEDFDRFVEFTGRYDA
                     DLSGVIRMDLRYENMIICALSDEEMERRAEAARERAKAEAATQEAKD"
BASE COUNT          613 a         1313 c         1374 g          628 t
ORIGIN      
        1 tcggcgtcgg gctcggcgac gggcggcaga aacacttctt cctgccctac ccgcacaccg
       61 acttcatctt cgccgccgcc ggcgaggaga tcgggctcgt ggggctgctc ctgctgctgt
      121 cgcttttggg gttcattctc taccggggct tcaagattgc ggccgaccag ccggaccggt
      181 tcgggttcct gctggcgagc ggcgtggtgc tgtcgctgtt catcaatatc gcggtcaatg
      241 tcgcggtggt gacgagcctc atgccggtca ccgggttgcc gctgcccttc atttcctacg
      301 gcggctcatc gctcctcatc acgagcataa ctatcggcgt cctgctcaac ctgtcgcggc
      361 ggatggtggg gagggcgaag caatgacggg gcggacgcag gagtaccggc tggtgttcgc
      421 gggcggcggg acgggagggc acctcttccc ggcgattgcg atcgctgacc gcgtccgcga
      481 gctgcttgcg gggacggccc cggtggagat tctctttgtc ggcacgcggc gcggcctgga
      541 gtaccggatg cgcgacgccc tcggctatcc cctccacgtt atcaacgtcc gtggactggt
      601 gcgcgcgctg tcgccggcca acctgctggt gccgttcgtg ctcgtcaccg cgctcgtccg
      661 ggcctggctc ctgctcgggc gcttccggcc ggatgcggtg gtggggacgg gcggctatgt
      721 cgccctgccg gtgttgaaag ctgcccgctg gcgcggcgta cccaccgtgc tccaggaaca
      781 gaattccttc cccggcatca ccacgcgcca gggggcgaaa cacgcgcgca agatctacct
      841 ggggttcgac ggcgcgcggc agtacctccg cacggaggcg gaggtcctca cgaccgggaa
      901 cccggtgcgg cgggggctga gcggcggcga ccgcgccgcg gcggcggcgg ccttcgggct
      961 cgacccggcc aagaaaacga tcctcgtgct gggcggcagc cagggagcgc gggcgatcaa
     1021 cgaggcggtg ctccggagcc tcgccggcgg cgcgccggcg gacggctgcc agattctgtg
     1081 gcagaccgga aagagggatt acacggaggt ttctgcgaag gcaggcggca cggcagcgcg
     1141 ctgcgccctc tttccgtttg cccatgacat ggccgcggtc tatgccgcgg ccgacctggc
     1201 cgtcgcgcgg gccggggcgc tcacgctggc cgagctggcc gcctgcgcca tcccggccat
     1261 tctcatcccc tatccccacg cggccggaga ccaccagcgc aagaacgccc gcgacctcgc
     1321 cgccgcgggt ctcgcccggg tgatcgacga aaacgatctt ccccggcacg atttgatcgc
     1381 cgaggcggcc gacctgctgg aggcggccga gtaccggacc atgagagaga aactggagtc
     1441 cgcgcggcgt cagggacggc cggcgacgga catcatcgcc gaggacatca tcgacctcat
     1501 caaccgtgag agaaaggcgc gggcgtgaaa acggcagtca ccaccaaacc caaagaaatc
     1561 gcaccctttg cgaacgacag actcatgttt ggaaaaatcc gcaagctgta tttcgtcggg
     1621 atcggcggct ccggcatgtc ggggatcgcc gagatcctcc acaacctcgg cttcgaaatc
     1681 agcggctccg accagacggc ctcggacctc acccgctatt tggccgaacg cggcctgcgc
     1741 atccatgagg ggcacgacgc ggcgcacctc ggcgatgccg aggtggtggt gatttcgtcc
     1801 gccgtcggcg aggccaaccc ggaggtggtc gaggcgcgcc gccgcggcat ccccgtgatc
     1861 aagcgggccg aaatgctggg cgaactgatg cgcctgaagt actccatcgg ggtggcgggc
     1921 acccacggca agacgacgac gacctcgatg atcgggcgca tcctccagtc cggcgggctc
     1981 aagccgacca tcatcgtcgg cggaatcgtc gccgagctcg gcaccggggc gtcgctcggc
     2041 gaaggcgagt acctggtggc cgaggccgac gaattcgacc ggtcctttct cgcgatgttc
     2101 ccgaccatgg cggtggtgac caacctcgag gcggaccacc tcgactgcta ccgcggcctg
     2161 gatgacctcg ggcacgcctt cctcacctat atgaaccgcg tgcccttctt cggctcggtc
     2221 gtgctgtcgg cggacaacgc gcagctcatg cagatgcgcg accgcctcgc gcgcccctac
     2281 gtgaccttcg gcttttccgt cgaggcggac taccgcgcgg tggaggtgcg gctcgagccg
     2341 ggacggtcgg tcttctcgct ctaccggcgc gggcagtacc tcggcgacat cctcctgcgg
     2401 gtgccggggc gccacaacgt cgccaacgct ctcgcggcgg cggccgcctg caccgaactg
     2461 ggtgtgccgt tcgagctgat cgccgacggt ctgcgcggat tcaccggcgt cgggcgccgc
     2521 ttcgagatcg tcggcgaggc cggcggcgtg acggtggtcg acgactacgc ccaccacccg
     2581 accgaggtgg aagcgaccct gcgcacggcg cgggacgtgt acgggcggcg cgtgatcgtc
     2641 gtcttccagc cgcacctgtt cagccggacg cgggatttcg ccgatgaatt cgcgcaggcg
     2701 ctggccatcg ccgaccagtg cctgctcgtc gacatctacc cggcgcggga gaagccgatg
     2761 ccgggagtga cggccgatct gatccgggag aaagccgccg cgcgcggtct cggccaattc
     2821 acctgcatcg gcgagcggca gaacgcggcg cgggaagtgg tccagcgggc gcagccggga
     2881 gatatcgtca tcaccatggg ggccggctcc atcacgctgg agcggaagaa cattctggag
     2941 gcgctgaggg agcggtgggg ataagcggca gaaaataccg gttgttcgcc ctcgccggcc
     3001 tcctggtcgg cctggggctc acggtgacgg tcaatttcac cgatgcctgc cgcctgcgcg
     3061 cggtgacggt cgacggcaag ccggtcgaga actggcggca gcagtttgcg atgctccgcc
     3121 ccggctctct cgccgcccag ccggtcgagc ggctggcgaa gaagctgctg gccgacgagg
     3181 acgtctacaa agtcgacgtg cggttcgaat tgccgggggg gatcgatgtc cggaccaacg
     3241 agttccggcc cgtctgtttc gtggtgggga tggagacggg caagctgttc gggctgaacc
     3301 ggcgggcccg cctcattgcg ctcgacgccc cggacatcaa ctgggagcag ccggtgttca
     3361 ccggcctaac gacgggggcg ctccactcga tctgcgccga cccgcgggtg ctgccggtga
     3421 tcgaccagct cgaggacctg cgcgaatcgc gcgccgacct ctaccgcctg ttggacgaga
     3481 tcgatttcgg cgaacgcgag tacctggagg tgacgttcgc cggactcccc taccgcctgc
     3541 gggtgcggcc cgaccacgtg cttgaggatt tcgaccggtt cgtcgaattc acgggccgct
     3601 acgatgccga cctctccggc gtgatccgca tggacctgcg ctacgaaaac atgatcatct
     3661 gcgcactctc cgacgaggag atggagcggc gggcggaagc ggcccgcgag cgggccaagg
     3721 ccgaggccgc gacccaggag gcgaaagact gaccggcgat ggcgaccgag aacatcatag
     3781 ccgccctcga catcgggacc accaaggtgg tctgcctggt cgccgagatg gacaccgacg
     3841 ggcgaatcta cgtcatcggc cgcgccggag cccctcggcc ggggcttggc cgtggcacgg
     3901 tgatcatgat gttggcgggc ttgatgtc
//
LOCUS       DAOOKS010000091         3922 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_27930_length_3922_cov_3.126196, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000091 DAOOKS010000000
VERSION     DAOOKS010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3922
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27930_length_3922_cov_3.126196"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            334..792
                     /locus_tag="PK112_02275"
     CDS             334..792
                     /locus_tag="PK112_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10908.1"
                     /translation="MSQNLNEYLRAGIFGFYGKEADEYDRRNEVWMAGGDATVGWGPA
                     ELNLQYVERRDDNPGFSLPADANEVETRGGFAELIVWPKGDRSRWYGAAMFNYVESGL
                     SMEPFAQKMYRVVTGHVGYLLQTNLRLTLENSYDLEAEENSLLAGFVSAF"
     gene            complement(915..2090)
                     /locus_tag="PK112_02280"
     CDS             complement(915..2090)
                     /locus_tag="PK112_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10909.1"
                     /translation="MNRTQSNSQRGSALLIALVLTGMLSLIALMAIDNSQTEIDLSFN
                     KINSDRSFYIAEAGVQHAHTVINATPGWNAGLAGVAFAGGAYSVSVRDSLSEPALADS
                     VVLLSQGEVNGARSTIEATLAPEPFYPFRYGLFADSGVAIGNSVSTDSWNSDSASYAG
                     SAVNAYGSVGSNGPIVIENNPTISGDVYTAQAGAITITGTGRVLGDTSSQAPELDFSK
                     LISPTQFADAEAVNRAPLGMSGSFTYNNGSRDLRIGAGQTMVLESGTYVLDDILLEQS
                     AKLVLAPGAQVELYVTGNIDLRNKATVNSGGKPGNFRVYSRGTRFTMGQSSEFTGAVI
                     APNVNFNLAQYTDFYGSLMSNRIDLSNNPRFHYDRSLGLIINGTTGRMIQVAWRELE"
     gene            complement(2403..2891)
                     /locus_tag="PK112_02285"
     CDS             complement(2403..2891)
                     /locus_tag="PK112_02285"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HPC10910.1"
                     /translation="MTNSENLLYMMRINQRALKALLDDITEEESMVRGRDSLLHIRWL
                     TGHLVYNVNHVLAMIGSLPVMLPSSYKQLFDRGSAITNDPKAYPSLAALRSELLDLHD
                     RLIDVLPGVSDDDLDEPLPKDAGFNAVRLNAASFMCMHTFYHCGQIAMIRRVLGRERS
                     FG"
     gene            3205..3651
                     /gene="msrB"
                     /locus_tag="PK112_02290"
     CDS             3205..3651
                     /gene="msrB"
                     /locus_tag="PK112_02290"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002812087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HPC10911.1"
                     /translation="MGEKIQKTDEEWRAQLTPEQYRITRECGTEPPFSGKYYYHREDG
                     TYRCIGCGNALFTSGTKYESGSGWPSFWQPASPQAIEQKTDRSHGMARTEVVCGKCGA
                     HLGHVFPDGPRPTGLRYCINSVALDFTKDTAAADSADAAADSSKGD"
BASE COUNT          693 a         1100 c         1465 g          664 t
ORIGIN      
        1 gcgaacgggg cgaggtcgcg ggggtggaag acgcctacct gatgttcaac gacctgttcg
       61 ggcaggatct cgatctctat gtcgggcagt tccaggtctc cgaccccctc ttcaagcgcg
      121 agctgcggtt gacgttcgac gattacgcgg tgtacgtcgc cagccccggc gcttcgggca
      181 tcaacctgaa atacgaccgg gggctaatgg tcacctacgg ggcgccgacc ggcaccgacg
      241 tcgcggtcga gctggtcaac gggagcgggc tggcggaagg cgaggggaac cggcacgtac
      301 gaccgcgaca agtacaagaa cctgctcgcg cgggtgtcgc agaatctcaa cgagtacctg
      361 cgcgccggca tattcggatt ctacggcaag gaggcggacg agtacgaccg ccgcaacgag
      421 gtctggatgg cgggcgggga cgcgacggtc gggtggggtc cggccgagct caaccttcag
      481 tatgtggagc ggcgggacga caatcccggg ttctcgctgc cggcggatgc caacgaggtc
      541 gagacgcgcg gcggattcgc cgaactgatc gtctggccga agggcgaccg gtcgaggtgg
      601 tacggggcgg cgatgttcaa ctatgtggaa tcggggctga gcatggagcc gttcgcgcag
      661 aaaatgtacc gggtcgtcac cgggcacgtc ggctacctcc tgcagacgaa tctgcgcctg
      721 acactggaga acagctacga tctggaggcc gaggagaact cgcttttggc gggattcgtg
      781 agcgcgttct agcgggcggg ggtaagggag agaaatgaga agccgggcgg gtggtccgcc
      841 cggcttcgcc gcgttgaagg gtcgttgctg aaccgcttac aggaagttcc cttttagagg
      901 aagaagggca tattttactc cagctcccgc caggcgacct gaatcatgcg gccggtggtg
      961 ccgttgataa tcaggccgag gctgcggtcg tagtggaagc gggggttgtt gctgaggtcg
     1021 atccggttcg acatgagcga gccgtagaag tcggtgtact gggcgaggtt gaagttgacg
     1081 ttgggggcga tgacggcgcc cgtgaattcc gagctctggc ccatcgtgaa acgggtgccc
     1141 ctggagtaga cgcggaagtt gcccggcttg ccgcccgagt tgacggtggc tttgttgcgc
     1201 aggtcgatgt tgccggtcac gtagagctca acctgcgcgc ccggggcgag caccagcttg
     1261 gccgactgct ccagcagaat gtcatcaagg acgtaggtgc cgctctcgag gaccatggtc
     1321 tggccggcgc cgatgcggag gtcgcggctg ccgttgttgt aggtgaagct gcccgacatg
     1381 ccgagggggg cgcggttgac ggcttcggcg tcggcgaact gggtcgggct gatgagcttg
     1441 ctgaaatcaa gttccggcgc ctgggaggag gtgtcgccga gcacgcggcc ggtgccggtg
     1501 atggtgatgg cgccggcctg ggccgtgtag acatcgccgc tgatggtcgg gttattctcg
     1561 atcacaatgg ggccgttgga tccgaccgat ccgtaggcgt tgaccgccga accggcgtag
     1621 ctggccgaat cggagttcca ggagtcggtg ctgacgctgt tgccgatcgc cacgccgctg
     1681 tcggcaaaga ggccatagcg gaacgggtag aacggctcgg gggcgagggt ggcttcgatg
     1741 gtggagcggg cgccgttgac ctccccctgg gagagcagga cgacgctgtc ggccagggcg
     1801 ggttcgctca ggctgtcgcg gaccgacacc gagtacgcgc cgccggcgaa ggcgacaccg
     1861 gccagcccgg cattccagcc gggggtggcg ttgatcacgg tgtgggcgtg ctgcacgcct
     1921 gcttccgcaa tatagaagga gcggtcggaa ttgatcttgt tgaaggacag gtcgatctcg
     1981 gtctgcgagt tgtcgatcgc catcagggcg atcagcgaga gcatcccggt gagcaccagc
     2041 gcgattaaga gtgcgctgcc tctctgactg ttgctttgcg tgcggttcat catttctcaa
     2101 actccctttc actgcggttg agctatggcc tacgtggggg ccatgagggc aaacgcggtg
     2161 ccaagtcgga aagggagggg ccaggggggc caagtggttg accgcaggtt ggttggaggc
     2221 gagatcagag attgcacaga gaaccggact tcaccccggg cgtccgatct cctgcatcgc
     2281 ggtgcgcagt catgcgcata cccggcgccg gtgcggcaga aagcggccgg gatttgcaca
     2341 gcgcggccga gggtgctcgc ggcgggcggg gcgggagcgg gggcggcccg cggccgcgac
     2401 cctcatccga aagaccgctc gcgcccgagg acgcggcgga tcatggcgat ctggccgcag
     2461 tggtagaacg tgtgcatgca catgaaggag gcggcgttca ggcgaacagc gttgaaaccg
     2521 gcgtctttcg gcagcggctc gtcgaggtcg tcatcggaca cgccggggag aacgtcgatc
     2581 aggcggtcgt gcagatcgag cagttcgctg cgcaaggcgg ccagcgacgg ataggctttg
     2641 ggatcattgg tgatggcgct gccccggtca aagagctgct tgtaggagga cgggagcatc
     2701 accggcagcg agccgatcat ggccaggacg tggttgacgt tgtagacgag gtggccggtg
     2761 agccagcgga tgtgcaggag gctgtcgcgt ccgcggacca tggattcctc ctcggtgata
     2821 tcgtcgagca gagctttgag agcgcgctgg tttatgcgca tcatgtagag cagattctcg
     2881 ctgttggtca tggcgtcctc gtttcagtcg ttgcggcgca gagagtgctg tccgtgcggg
     2941 tatgatacgc cgggggccgc gcgggggcaa gggggaagat ggggcgcgtt ttcggaaacc
     3001 gccggcagcg gggagtgtta cagcagtgcg gcggccgcag cagccgccgg cggcaaggaa
     3061 aaaccgatgg caagaaccgg actggcaatg atcgcatgcg cggcggcgct cggcctcttc
     3121 tcggcgattg ccggcacaca ggctggcgcg ccggcgaagg agacagcggc caaggccgga
     3181 caggcgaaag ccaaggggaa cgcgatgggc gagaagatac agaagaccga cgaagagtgg
     3241 cgggcacaat tgacgcctga gcagtaccgg atcacccggg agtgcggcac ggaaccgccc
     3301 ttcagcggca agtactacta ccaccgcgag gacggcacct accgctgcat cggctgcggc
     3361 aatgcgctgt tcacctcggg gaccaagtac gaatccgggt cgggctggcc gagtttctgg
     3421 cagccggcgt cgccgcaagc gattgagcag aagaccgacc ggagccacgg catggcgcgg
     3481 acagaggttg tgtgcggaaa gtgcggggcc cacctcgggc atgttttccc cgacgggccg
     3541 cggccaaccg ggctgcgcta ctgcatcaac tcggtggcgc tcgatttcac gaaagacacg
     3601 gccgctgcgg acagcgccga cgccgcagcg gactcctcga aaggggactg aggcgggttg
     3661 ccggccgcgc tccgggcggt tgcccgaccg cgcggcgtgc tccggctgac aggcgatggc
     3721 gcgccggggg actgtatgag ccgccggtcg cattgcgcca accggtcgcc gtcaggcgcc
     3781 aggaggcgcc tgaccccggt gacccgcggg ggaaattcta tttcatgcgc acgttgaggg
     3841 ctctgaagac gaacgaccac tggtcggtct gttcttcaat ctgcttgctg atcggtttgc
     3901 cctggccgtg gcccgccttc gg
//
LOCUS       DAOOKS010000092         3897 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_28164_length_3897_cov_3.209266, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000092 DAOOKS010000000
VERSION     DAOOKS010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3897
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28164_length_3897_cov_3.209266"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2229)
                     /locus_tag="PK112_02295"
     CDS             complement(<1..2229)
                     /locus_tag="PK112_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10912.1"
                     /translation="MKRLGVLIVGVLACWIGPDPARARMTEAADGLRGYRRPLAAVSP
                     AHMLAAHRAGRIELAVANNGTFGKEYHPGEASDAFTGEAIPLSCQYPKGSLVAYLFGG
                     AFWIGAVVGRDTLVSVGADGWQQVYEFFPDPAPFGNMVRRSIRDPEDSLAVSEEDYIS
                     VYTDTFPELVNPDPFAAVHTPLCVQVTQASYAWSYEYTEDFVLFDYRIKNIGRETLRQ
                     VYMGIYVDDMICYDCFGSNLGYTDDHSGFLHTYPVRYGRCEYLDTVFLAWQADNDGDL
                     ETIFSDGRRHPCPDVIATRIIRTPAEDLDVSFNWWIGNSNAQLDFGPREREFVGRWKE
                     PVRDFGTGGLGTPEGDRNKYYVLRNQEFDYDPIFTATISSSDSRWLYPDPSLAADYAD
                     GYDTRYLLSFGPFDIQPGRSLPVSFAYMAGEHLHRSPDNAAHLPARPDLYYANLDFSD
                     LALNAGWASRVYDNPGVDTDGDGYRGRFRLMKTRADGTVECLPDGIYIGPPGDSLQGG
                     EPRLDTVWYQGDGVPDFRGAAPPPPPEFRLASPSAGTILVRFNGLLSETAQDQFSGLY
                     DFEGYRVYLGRDDREASFSLAASYDREDFNKLVWDADASEWRLFDPPYTLAELRCLYA
                     DSCGDATFDPLRYTRVNRFVHPRYPDSLFCFEKQDYNAAGSGDLGAIVKSYPDQPYPS
                     SPDPALVRPDELTAEGRLKYFEYEFTITGLLPSVPYWVNVTAFDFGSPSTGLPALESS
                     VTV"
     gene            2476..3126
                     /locus_tag="PK112_02300"
     CDS             2476..3126
                     /locus_tag="PK112_02300"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HPC10913.1"
                     /translation="MKSIEGTKAAPGLNQRKRQFYEEWSLSLGRVCVRLGFKPNYITA
                     VSLLCAIISGVFFWRGQWLWGVVWMVITSFTDMLDGSTARAGKMGTVFGGILDHVSDR
                     YGEFFILAGITLSGRVAPGWGLFALFGMIIASYTRAAAESMGKIENCAVGIMGRLEKF
                     ILIIIGSLLEYFLPVGAFPRSGWLELALIVVGATSYITSVQRMVYTYKVLGDKREV"
     gene            3163..>3897
                     /locus_tag="PK112_02305"
     CDS             3163..>3897
                     /locus_tag="PK112_02305"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HPC10914.1"
                     /translation="MITALGNPVYDLITTRKVRPTGRVLSGCSTNAALALAKLGERVR
                     LVGAVGEDFRPQLAADLARHGIDHVIIPSAESGGFALNYYDDHGNRTLDLIGRAAPID
                     PIDPAWFADAEAVLVGPILGEISLDNIRRIRRLYDGFFFCDPQGLLRGADAGGRIYHE
                     KAPGIDDILGLFDVVKPNELEARVLTGVDCREDPYRAARLIQKAGPRVVIVTLAELGS
                     VIYDGREFIDIPPYTDIALLDSTGAGD"
BASE COUNT          657 a         1253 c         1334 g          653 t
ORIGIN      
        1 gaccgtgacc gaggactcca gggcgggcag ccccgtgctc ggcgaaccga aatcgaacgc
       61 ggtcacgttc acccagtagg gcacggaggg gagcaggccg gtgatggtga attcgtattc
      121 gaagtatttc agtcgcccct cggccgtcag ttcgtccggc cggaccagcg cgggatcggg
      181 gcttgaggga tacggctggt cgggatagct cttgacgatg gcgccgagat cgcccgaccc
      241 ggcggcattg taatcctgct tctcgaagca gaagagggag tccgggtagc gcgggtggac
      301 gaagcggttg acccgggtgt agcgcaacgg gtcaaacgtc gcatcgccgc acgaatcggc
      361 gtacagacag cgcagctcgg ccagcgtgta ggggggatcg aacagccgcc actcggacgc
      421 gtcggcatcc cagacgagct tgttgaagtc ctcacggtcg tacgaggctg cgaggctgaa
      481 actggcctcc cggtcatcgc gccccaggta cacccggtat ccctcgaaat cgtacagccc
      541 ggagaattga tcctgggcgg tctcggacag caggccgttg aagcggacca ggatcgtgcc
      601 ggccgaggga gaggcgaggc ggaattcggg gggcggaggg ggggcggccc cccggaaatc
      661 cggcaccccg tcgccctgat accacaccgt atccagcctc ggctcgcccc cctgcagact
      721 gtcgcccggc ggcccgatgt agatcccgtc gggcaggcac tccacggtgc cgtcggcccg
      781 cgttttcatc aggcgaaatc tcccgcggta cccgtcgccg tcggtgtcca cgccggggtt
      841 gtcgtagacg cgggaggccc agccggcgtt gagggcgaga tcggagaaat caaggttggc
      901 gtagtagagg tcgggccgcg cggggagatg ggcggcgttg tcgggggagc ggtggaggtg
      961 ttcgccggcc atgtaggcga acgagaccgg gagcgagcgg ccgggctgga tatcaaacgg
     1021 cccgaacgag agcaggtagc gggtgtcata gccatcggcg taatcggccg ccaggctcgg
     1081 gtccgggtag agccagcggc tgtcggagct tgaaatggtc gccgtgaaga tcgggtcgta
     1141 gtcgaactcc tggttgcgca gcacgtagta cttgttccgg tccccctcgg gcgtccccag
     1201 tccgcccgtg ccgaaatccc gcaccggctc cttccagcgc cccacgaact cccgctcgcg
     1261 cggaccgaaa tcaagctgcg cgttgctgtt cccgatccac cagttgaaac tcacatcgag
     1321 atcctccgcc ggcgtccgaa tgatgcgggt cgcgatcacg tccgggcagg gatgccgccg
     1381 gccatccgag aatatcgtct ccaggtcgcc gtcgttgtcc gcctgccagg cgaggaagac
     1441 ggtgtcgaga tactcgcagc ggccgtaccg caccgggtag gtgtggagaa atccgctgtg
     1501 gtcgtcggtg taccccaggt tgctgccgaa gcagtcgtag cagatcatgt cgtcgacgta
     1561 gatgcccatg tacacctggc gcagcgtctc gcggccgatg ttcttgatgc ggtagtcgaa
     1621 gaggacgaaa tcctcggtgt actcgtacga ccaggcgtac gacgcctgcg tcacctgcac
     1681 gcaaagtggg gtgtggacgg cggcgaacgg atcggggttg accagttccg ggaaggtatc
     1741 ggtgtaaacc gagatgtagt cttcctcgga gacggccagg gagtcttcgg gatcgcggat
     1801 ggagcggcgg accatgttgc cgaagggggc ggggtcgggg aagaactcgt acacctgctg
     1861 ccagccgtcg gcgcccaccg acacgagggt atcgcgtccg accacggcgc cgatccagaa
     1921 ggcgccgccg aagaggtagg cgaccagcga acctttgggg tactggcagg agagcgggat
     1981 ggcctcgccg gtgaaagcgt cggaggcttc gccgggatgg tactccttgc cgaaggtgcc
     2041 gttgttggcg acggccagtt cgatgcggcc ggcccggtgg gcggcgagca tgtgggctgg
     2101 cgagacggcc gcaagcggac ggcggtaccc gcggaggccg tccgcggctt ccgtcatgcg
     2161 ggcccgcgcc ggatccgggc cgatccagca ggcgagcacg cccacgatca ggactcccag
     2221 tcgtttcatg gaactacgcc gcgcggcggc gggcggaata cgtatgcgac cgccgccggc
     2281 cgtctactcc aacgaaaaca gttcaacgcc gcctggccaa gcggttttcc gcggccgcgc
     2341 taagcccttg ccgggcaaga tggtttttgc gggcggggag caccccgcgg ggcggacacg
     2401 ggcccaaaaa aaccgccgcg cgcgccgggt tgactcgttg acctccccca aaaagcgcat
     2461 atattactcg ccgggatgaa aagcatcgaa gggacgaaag cggcgccggg actcaaccag
     2521 cgcaaacggc agttttacga agagtggtcg ctctcgctcg ggcgggtgtg cgtccggctc
     2581 ggcttcaaac ccaactacat caccgccgtc agcctcctct gcgcgatcat ctccggcgtc
     2641 tttttctggc gggggcagtg gttgtggggg gtggtgtgga tggtcatcac gtcgttcacc
     2701 gacatgcttg acggctcgac ggcccgcgcc gggaaaatgg gcactgtctt cggcggcatc
     2761 ctcgaccacg tctccgaccg ctacggcgag ttcttcattc tcgccggcat cacactctcc
     2821 ggccgggtgg ccccggggtg gggtctgttc gcccttttcg gcatgatcat cgcctcgtac
     2881 acgcgggccg cggccgagtc gatgggcaag attgagaatt gcgccgtcgg catcatgggg
     2941 cggctcgaga aattcatcct catcatcatc ggctcgctgc tggagtattt tctccccgtg
     3001 ggcgctttcc cgcgcagcgg ctggctggag ctggcgctga tcgtggtcgg cgcgacctcg
     3061 tacattacct ccgtccaacg gatggtgtac acctacaagg tgctgggcga caagcgcgag
     3121 gtgtagacgc cgccgcggga gcccggaaag gatttttccc ccatgatcac cgcgctcggc
     3181 aacccggtct acgacctcat cacgacccgg aaggtccggc ccaccggccg cgtgctctcc
     3241 ggctgctcga ccaacgccgc gctcgcgttg gcgaagctgg gcgagcgcgt ccgcctcgtc
     3301 ggcgccgtcg gcgaggattt ccgtccgcag ctggccgccg acctcgcacg ccacgggatc
     3361 gaccacgtca tcatcccgtc agcggagagc ggcggtttcg ccctgaatta ctacgacgac
     3421 cacgggaacc ggacgctcga cctgatcggc cgggccgcgc cgatcgaccc gatcgacccg
     3481 gcctggtttg ccgatgccga ggcggtgctg gtcggtccca tcctcggcga gatttcgctc
     3541 gataatatcc ggcgcatccg ccgcctctac gacgggttct tcttctgtga cccgcagggc
     3601 ctgctccgcg gcgccgatgc cggcgggcgc atctatcacg agaaagcccc cggcatcgac
     3661 gacatcctcg gcctcttcga cgtcgtcaag cccaatgagc tcgaggcgcg cgttctcacc
     3721 ggcgtcgact gccgcgagga tccctaccgg gcggcccggc tcatccagaa ggcggggccg
     3781 cgggtggtga tcgtcacgct cgccgaactc ggctcggtca tttacgacgg ccgggagttc
     3841 atcgacatcc ccccttacac ggacatcgcc ctgctcgatt ccaccggcgc cggcgac
//
LOCUS       DAOOKS010000093         3895 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_28183_length_3895_cov_3.573958, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000093 DAOOKS010000000
VERSION     DAOOKS010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3895
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28183_length_3895_cov_3.573958"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1497)
                     /locus_tag="PK112_02310"
     CDS             complement(<1..1497)
                     /locus_tag="PK112_02310"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPC10915.1"
                     /translation="MLGYIVDFMLRRRYAVLAATAVLCLFGLAALWHLPFDAFPDTTP
                     VLVQVNVAAPGWAPEDLERLVAYPVEQALTGLTGLAEVRSVTRYGLCQVTTIFDDGVD
                     LYLARQQVSERLPGIALPDGIGAPELGPVSTGLGEIFHYIVVADSGDVTDPRTVQDWL
                     IKPQLLAVPGTAEINSWGGFVKEYLILFDPTRLARYNLPLDDMAAAIREQLGNVPGGQ
                     IIRGGEMTLVRGIGQVENVGQIENIVVAVVEGTPIAVRDVAEVVIGHELRRGATTYNG
                     RGEAVLGLGFMVTGENPAEVTERLARRLGEAAGGLPAGVRAVPVYERTKMVGEVLSTV
                     EHNLFYGAVLVVAVLLAFLGNLRAGLIVASAIPLAMLFAFDLMSRAGITGSLMSLGAI
                     DFGLAVDNGVIQVENSIRRLSQAGGSASRLHVIRDAILEVRKPTLFGEIIIIIVYLPI
                     LTLEGVEGKLFRPMALTVVFMLTGSLILSFTVLPALIGTLLHKGVRARE"
     gene            complement(1506..3005)
                     /locus_tag="PK112_02315"
     CDS             complement(1506..3005)
                     /locus_tag="PK112_02315"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPC10916.1"
                     /translation="MRLKSGLLAALCALVVFGCGGAEQPAGEADAHAGHAHGPGGHAE
                     AAPIEPPAAQADDWCAEHRVPESQCTACDPALIAAFIAAGDWCEEHGLPESHCRLCNP
                     GLGFPQEEPASSAADIAAEEIRVSLFFRPNALVCATNDALIQLATARTAERAGLTVQQ
                     VRAAELETTIEAPAEVVFDETRTTAVATTVAALVARWVAAPGETVREDQVLAILQSPE
                     VAALESQLLSAHADLLVHTNELARQEELRGRDLISDAELERQRALFAQAKAAYVAGRG
                     LLRAAGLSEDDLDEIIAEGRISSTFALRAPTAGLLVERRALPGELQEAGSALARLADP
                     AVMWIEARLTEEQLRQVAVGEPLTFASDGRGLSRVGGKIIWVARFLDPHSRTGTVRAQ
                     VVDHRHTLQAGEFGRVTIARTGHRRVALVPRDAVQWEGCCNVVFVREAADRYRPRKVE
                     FTDGAGPYYQVLRGLEPGEEVVVDGAFLLKTELKKASIGAGCCGLDPVG"
     gene            complement(3149..3412)
                     /locus_tag="PK112_02320"
     CDS             complement(3149..3412)
                     /locus_tag="PK112_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10917.1"
                     /translation="MVALVAASFALFVLGESFFEGCDEHCADENHEICTCAHCANSPR
                     LADESFPVAPADQGTHFPLCSRGTAVPEQLWIHAIDHPPRISC"
     gene            3682..>3895
                     /locus_tag="PK112_02325"
     CDS             3682..>3895
                     /locus_tag="PK112_02325"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPC10918.1"
                     /translation="MAREKKWWETFFEDFRPAFRREPPGETLKQARAIFRKLEMKKGM
                     RFLDCPCGFGRIGLPLARMGLKVTGVD"
BASE COUNT          655 a         1338 c         1292 g          610 t
ORIGIN      
        1 ctcgcgcgcg cggacccctt tgtgcagcag cgttccgatg agcgccggga ggaccgtgaa
       61 cgacaggatg agcgagccgg tcagcatgaa cactaccgtc agcgccatcg gccggaacaa
      121 cttcccctcg accccctcga gggtgagaat ggggaggtag acgatgatga tgatgatctc
      181 gccgaagagc gtcggcttgc gcacctcgag gatggcgtcg cggatcacgt gcaggcgcga
      241 ggctgaaccg ccggcctgcg acagcctccg gatcgagttc tcgacctgga tcacgccgtt
      301 gtcgacggcg aggccgaagt cgatggcccc gaggctcatc aggcttcccg tgatccccgc
      361 gcgcgacatg agatcaaacg cgaacagcat cgccagcggg atggcggagg cgacgatgag
      421 gcccgcgcgg agattgccga gaaacgccag cagcaccgcc accaccagca cggcgccgta
      481 gaagagatta tgctctacag tcgagagcac ttcgccaacc attttcgtgc gctcatagac
      541 cgggaccgcc cgcacgcccg ccggcagccc cccggccgct tcccccagcc ggcgggccag
      601 ccgttcggtg acctcggccg gattctcgcc ggtgaccatg aacccgagtc caagcaccgc
      661 ctcgccgcgc ccgttgtagg tcgtcgcgcc ccggcgcagt tcgtgcccga tcacgacctc
      721 ggcgacatcg cgcaccgcga tcggcgttcc ctcgaccacg gcgaccacga tgttctcgat
      781 ctggccgaca ttttccacct ggccgatccc ccgcacgaga gtcatctcgc ctccccggat
      841 gatctgcccg ccgggcacgt tgccgagctg ctcgcgaatc gccgccgcca tatcgtcgag
      901 cggcaggttg taccgcgcga ggcgggtcgg gtcaaagaga atgagatact ccttgacgaa
      961 cccgccccag ctgttgattt ctgctgtccc cggcaccgcc agcaactgcg gcttgatcag
     1021 ccagtcctgc accgtgcggg ggtcggtgac gtccccggag tcggcaacca cgatgtagtg
     1081 gaagatttcc cccaggccgg tggagaccgg ccccagttcg ggcgccccga tcccgtcggg
     1141 cagggcgatg ccgggcagcc gttcgctcac ctgctggcgg gccaggtaga gatcgacgcc
     1201 gtcgtcgaaa atcgtagtga cctggcacag cccgtagcgg gtgaccgagc gcacttccgc
     1261 cagcccggtc agccccgtga gcgcctgctc gaccgggtat gccacgagcc gttcaagatc
     1321 ctccggcgcc cagcccgggg cggcgacgtt cacctgcacc aggaccggcg tggtgtccgg
     1381 gaaagcgtcg aaggggagat gccagagggc cgccaggccg aacaggcaga ggacggcggt
     1441 ggccgcgagc acggcgtatc gccggcgcag catgaagtcg actatgtatc ccagcatact
     1501 ccccctcagc cgaccgggtc gaggccgcag cagccagcgc cgatgctggc ctttttcagt
     1561 tcggtcttca ggaggaaggc gccatcgact accacctctt cgccgggttc gagtccgcgc
     1621 agcacctggt agtacggacc ggcgccgtcg gtgaactcga ctttgcgcgg gcggtagcgg
     1681 tcggccgctt ctctcacgaa caccacgttg caacagccct cccactgcac ggcgtcgcgc
     1741 ggcacgagcg cgacccggcg gtggccggtc cgggcaatcg tgacacgccc gaactccccg
     1801 gcctggagcg tatgccggtg atcgaccacc tgcgcccgca ccgtcccggt gcgcgagtgg
     1861 gggtcgagaa accgcgcgac ccagatgatt ttcccgccga cccggctcaa cccgcggcca
     1921 tccgaggcaa acgtcagcgg ctccccgacc gccacctggc gaagttgttc ctcggtgagg
     1981 cgggcctcga tccacatcac cgcgggatcg gccagtcggg ccaaggcgct gcctgcctcc
     2041 tgaagttctc ccggcagcgc ccggcgctcg acgaggagtc cggcggtcgg cgcgcgcagc
     2101 gcaaaggtgc tggaaatgcg gccctcggcg atgatctcat cgaggtcatc ctcgctcaag
     2161 ccggcggccc ggagcagccc ccggccggcg acataggccg ccttggcctg cgcaaagagc
     2221 gcccgctggc gttccagctc ggcgtcgctg atcaggtcgc ggccgcgcag ctcctcctgc
     2281 cgggccagct cgttggtgtg cacgagaaga tcggcgtggg cggagagcag ttgcgattcc
     2341 agcgcggcca cctccggcga ctgcaggatc gcgagcacct ggtcctcgcg caccgtttcc
     2401 cccggcgcgg ccacccagcg cgcgacgagc gccgccacgg tggtggcgac cgcggtcgtc
     2461 ctggtctcgt cgaacacgac ctccgccggc gcctcgatcg tcgtctcgag ttcagccgcc
     2521 cgcacctgct gcaccgtcag tccggcgcgc tccgccgtcc gggctgtggc aagctggatc
     2581 agcgcgtcat tggtcgcgca gaccagggca ttggggcgga agaagaggga gacccggatt
     2641 tcctcggcgg cgatatccgc cgccgaactc gccggctcct cctggggaaa gcccagcccc
     2701 gggttgcaga gccggcagtg cgactcgggg agaccgtgct cctcgcacca gtcgccggcc
     2761 gctatgaacg cggcgatcag ggcggggtcg caagccgtgc actgcgattc cggcacccgg
     2821 tgttcggcgc accagtcgtc ggcctgcgcc gccggcggct cgatgggggc cgcctccgcg
     2881 tgcccgcccg gtccgtgagc gtgccccgcg tgggcatcgg cctccccggc cggctgttct
     2941 gcgccgccgc atccgaacac caccagggcg cacagcgccg ccaagagccc tgacttcagt
     3001 ctcaacgcgt aactcatgtg tgcagatctc catttctcgt tcaggtgtga ggccgcgccg
     3061 ccggccgcac ctgggttcgg caattcgttt tggggaagac aaggacgcgc ggacgccgcc
     3121 gggaccgggc ttggccccgc ggacgcgctc agcaggagat ccggggagga tggtcgatcg
     3181 cgtggatcca gagttgctcg ggaacggcgg ttccgcgaga gcacaggggg aagtgcgtac
     3241 cctggtccgc gggcgcgacc ggaaacgact cgtccgccag ccggggggaa ttggcgcaat
     3301 gggcgcaggt gcagatttcg tggttctcat cggcgcagtg ctcgtcgcac ccctcaaaaa
     3361 acgactcgcc gagcacgaaa agcgcaaagg atgccgccac caacgccacc accgggatca
     3421 gtatcccggc acaagagaga tctggtcggc ggcgtctcat gatagcctgt aaccgggccg
     3481 gccccggttg gttctcttga agttaggcta tagtatacgt aattgctccg ctttcggcaa
     3541 gagaaaagct tgctaccggc cgggggttcg gaaggtggga cttgtacttg actggcccga
     3601 cccttctgcc ttgctttccc cgtcggcatt cacgcagagt tctgaatcgg cgccttgtcc
     3661 agcagtgaaa agggagatgg tatggcgaga gagaagaagt ggtgggagac atttttcgag
     3721 gactttcgac cggcttttcg acgcgaaccg cccggagaaa ccctcaagca ggcgcgcgcc
     3781 atctttcgga agcttgaaat gaagaagggt atgagatttc tggactgccc ctgcgggttc
     3841 gggagaatcg gcctgccgct ggccaggatg ggtctcaaag tcaccggcgt cgatc
//
LOCUS       DAOOKS010000094         3894 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_28193_length_3894_cov_3.364157, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000094 DAOOKS010000000
VERSION     DAOOKS010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3894
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28193_length_3894_cov_3.364157"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="PK112_02330"
     CDS             <1..588
                     /locus_tag="PK112_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10919.1"
                     /translation="AMARGDLRGAIGMARQALARAPYAAGPNLVLVRALAADTAVSDD
                     SLAGEIARAAQRARDDIYLLQMGAELLLNRGALAPASRFARRALEAEGPPIEMDHEAF
                     RHDFRNSRDKLNSQRAAAAVQLAYIAGNGNDIESAIAFARRAVELAPHQAEGYLNLYS
                     GLTAAGRTAEAGALLKEMAERFPDNRFVREMLRRR"
     gene            complement(857..932)
                     /locus_tag="PK112_02335"
     tRNA            complement(857..932)
                     /locus_tag="PK112_02335"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(897..899),aa:Ala,seq:ggc)
     gene            complement(1009..2517)
                     /locus_tag="PK112_02340"
     CDS             complement(1009..2517)
                     /locus_tag="PK112_02340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPC10920.1"
                     /translation="MLAAEVCQSFLRQQLIPVGSSGEHQRIIEAHRHRFFLVGAIGTF
                     MSTLDGSILNVALPTIAADLGADIHLVAWVVLAYTLTIISLMLIFGAWTERKGYDFAY
                     RFAYSFFVLGSAACALSPSIHSLILARVVQGIGSAMFQAVGPGLVTAVFPAEQRGRGI
                     GMMVMMVAAGLMAGPPLGGFILQFLPWQWIFVINLPIGAVGIALAARYFRLLPKPEDD
                     RPIYLAGGAAIATALVAGTLALLMVSEHALRDWQVWAPALAAAGAFLLFLRYESRPAR
                     HMIGLDMFRNRQFSTAIAAAILMFVTMAGGAILLPFYFESVKGYGPSRIGLFLVIIPA
                     IMFVVAPLSGRISDRIGSLFLTTSGAAVAAFGQYLLLHLDAASSDAFIMLVLAVIGLG
                     AAIFNTPNSSSIMGSVGPTERARASSIIGTARNIGNSIGIAMATALFAYFRSRRAVSL
                     GDPAALFVAAFHDVVKVSLGVALLTVPLCLTRHNRPRPGQGGGAPEPPAAGV"
     gene            2640..3191
                     /locus_tag="PK112_02345"
     CDS             2640..3191
                     /locus_tag="PK112_02345"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPC10921.1"
                     /translation="MDDFVRGSDAAFTELVHRYQRKVYSLAYQVLGNHLDADEVVQET
                     FVRIYRRRKELAEVKFFSTFLLRIASNYAIDVLRKQRGHSRMPEDSSSLPGEVQIDLA
                     RQVATPSEEFENRALLEEIKRALRELPPRQQLTAILHDIEGYSKAEIAAMLGCPEATV
                     RSNLHIARSKLRKALKKRLRKGS"
     gene            3193..3771
                     /locus_tag="PK112_02350"
     CDS             3193..3771
                     /locus_tag="PK112_02350"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HPC10922.1"
                     /translation="MTCSDVVARLDDFLDGELSAEAAGRVRDHLADCAACRREHDLAV
                     QVRGLLGSAAAPDPGDEYFAESESHILARTVLAGDPEAVRRGGGEGAAPRSSTALLRA
                     LVSVAASLAILFAAIYLGSSRELLISRTIGPEIPVLATAEVRSELGDSPVFTREDRAR
                     ITRGMMLIGGPGILGRFTALPQIVKTNPLDES"
BASE COUNT          739 a         1317 c         1232 g          606 t
ORIGIN      
        1 gccatggcgc gcggcgatct ccgcggcgcg atcggtatgg cgcggcaggc gctcgcccgc
       61 gccccctatg ccgccggtcc caacctcgtt ctggtgcgcg ccctcgccgc cgacacggcc
      121 gtgtccgatg actcgctggc gggggaaatc gcccgcgccg cgcagcgcgc ccgggacgac
      181 atctacctgc tgcagatggg ggcggagctg cttttgaacc ggggcgcact cgcgcccgcc
      241 agccgtttcg cccgccgcgc gctcgaggcg gagggccccc cgatcgaaat ggaccacgag
      301 gcgtttcgcc acgacttccg caacagccgc gacaaactca acagccagcg cgccgcggcc
      361 gctgttcaac tggcctacat cgccggcaac ggcaacgaca tcgagtcggc catcgccttt
      421 gcccgcaggg ccgtcgagct ggcgccgcac caggccgagg gatacttgaa tctgtattcg
      481 ggtctgacgg ccgccggacg gacggccgaa gccggggcgc tgctgaaaga gatggcggaa
      541 cggtttcccg acaaccgctt tgtccgcgag atgctcaggc gccgctgagc cccccacagc
      601 actgcctccg atgggcaaaa aaagaagggc ggatatgaat ccgcccctac ggtagagaat
      661 gcccatttcg ctccgcgccc ggcgccttgc ggctccgggg cacttcccgc gcggcccgac
      721 cagcgggccc tggtcattct ccataagaag tatcggcgcc ccggggcgcc gacttgagcg
      781 gaaaactcga tctttttctc cccgtgcaca aaaaaagacc gggcccggat ttgtcgccgg
      841 gcccggtcgc gggatttggt ggagccaggg ggagtcgaac ccccggcctc ttgaatgcca
      901 ttcaagcgct ctcccaactg agctatggcc ccaaatgacc tgtaggttcg ccagtatacg
      961 ccctccccga gaggttgtca actgcatttt acggcgccgg cggacctgtc agactccggc
     1021 ggcgggaggc tccggcgctc cgcccccttg tcccggacgc ggccggttgt gccgcgtgag
     1081 gcacagcggc accgtcaaca gcgccacccc gagcgagacc ttcacgacat catggaaggc
     1141 cgcgacgaac agggcggccg ggtcgccgag actgaccgcc cgccgcgacc gaaaataggc
     1201 gaagagagct gtcgccattg cgatcccgat cgagttgccg atgttgcgcg cggtcccgat
     1261 aatgctgctg gcccgggccc gctccgtcgg tccgaccgac cccatgatcg acgaggagtt
     1321 gggcgtgttg aagatggccg cgcccaggcc gatgaccgcc agcacgagca tgatgaaagc
     1381 gtctgatgaa gcggcatcca ggtgcagcag cagatactgc ccgaaagccg ccaccgccgc
     1441 tccgctcgtc gtgaggaaaa gagacccgat gcggtcggag attcgccccg acagcggcgc
     1501 caccacgaac atgatggcgg ggatgatcac gaggaacagg ccgatgcggc tggggccgta
     1561 ccccttgacg ctttcaaaat agaacggcag gagaatggcc ccgccggcca tggtaacgaa
     1621 catcaggatc gccgccgcaa tcgcggtgga gaactggcgg ttccggaaca tgtccaggcc
     1681 gatcatgtgg cgcgccgggc ggctctcgta gcgaaggaac agcaggaacg cccccgccgc
     1741 cgccagcgcc ggcgcccaaa cctgccagtc gcgcagcgcg tgctccgaaa ccatcaacaa
     1801 cgccagtgtg ccggccacca gggcggtcgc gatcgccgcc ccgccggcca ggtagatcgg
     1861 gcgatcgtcc tccggcttcg gtaggagccg gaaatagcgg gccgccagcg cgatccccac
     1921 ggcgccgatg ggaagattga tcacgaaaat ccactgccac gggagaaact gcaaaatgaa
     1981 cccgcccagc ggcggcccgg ccatcagccc ggccgccacc atcatcacca tcatcccgat
     2041 ccctctcccc ctctgttccg ccggaaagac ggccgtcacc agacccggtc cgaccgcctg
     2101 aaacatcgcc gaccctatcc cctgcactac ccgggcgagg atgagcgagt ggatcgacgg
     2161 gctcagcgca caggccgccg acccgagaac gaaaaacgag taggcgaacc ggtaggcgaa
     2221 gtcgtacccc ttgcgctcgg tccaggcgcc gaagatgagc atgagcgaga tgatcgtcag
     2281 cgtataggcg aggacaaccc atgccaccag gtgaatgtcc gcccccaggt cggcggcgat
     2341 cgtgggcagg gccacgttga ggatcgaccc gtccagggtc gacatgaagg tccctatggc
     2401 ccccacgaga aagaatcggt gtcgatgcgc ctcgatgatg cgctgatgtt cgcctgaact
     2461 gcccacgggt ataagttgtt gccgtaaaaa agattgacaa acttcagcgg caagcatatg
     2521 cttgccgtcg gggatagtca acagaatttt caccccgatg attgcacagg tggcgatgcc
     2581 gtacgtctct tcgatacatc atgacttcgg ggattatgca taagactgtc ccggagttag
     2641 tggatgattt cgtccgaggg agtgacgccg ccttcactga acttgtccac cggtaccagc
     2701 ggaaggtcta ctccctggcc taccaggtgc tcggcaatca cctggatgcc gatgaagtcg
     2761 tccaggagac ctttgtccgc atttatcgac gacgaaagga gctggccgaa gtaaagtttt
     2821 tctcgacctt cctgctgcgc atcgcgtcca actacgccat tgacgtcctg cgcaagcaga
     2881 ggggccacag ccggatgccg gaagattcgt cctccctgcc gggggaggtc cagatcgatc
     2941 tggcccggca ggtggccacg ccgagcgaag aattcgagaa cagggctttg ctggaggaga
     3001 tcaagcgggc gctcagggag cttcctccgc gacaacaact aaccgccatt ctgcacgaca
     3061 tcgaagggta ttcgaaggcg gagatcgcgg cgatgttggg ttgcccggag gcgaccgtcc
     3121 gctccaatct ccatatcgcc agatccaaac tgcgaaaggc cctgaagaag cgcctgagga
     3181 aagggagttg agatgacctg ctctgatgtc gtagcgcggc tggatgactt tctcgacggc
     3241 gagctgtcgg ccgaagccgc cgggcgcgtg cgcgaccacc tggctgactg cgcagcctgc
     3301 cggcgcgaac atgacctcgc cgtccaagtc cgcggcctgc tcggctcggc cgccgcgccc
     3361 gaccccgggg acgagtattt cgccgaaagc gagagccata tcctcgcccg cacggttctg
     3421 gccggcgacc cagaggcggt tcgccgcggg ggcggcgaag gcgccgcgcc gcgctcctcc
     3481 accgccctcc tgcgggcgct ggtgtcggtg gcggcctcgc tggcgatcct cttcgccgcc
     3541 atctacctcg gatccagccg ggagctcctg atcagccgga cgatcggccc ggagattccc
     3601 gtgctggcga cggccgaggt gcgctccgag ctcggcgaca gcccggtctt cacccgcgaa
     3661 gatcgggccc ggatcacccg cggcatgatg ctcatcggcg ggccgggaat cctcggccgt
     3721 ttcaccgccc tgccgcagat agtcaaaacc aacccgctcg acgaatcgta atagcctctg
     3781 ggaaggagtc tcggaattgt gaccagacta accgcctgcc tctccgcagt catcctcgcc
     3841 gctgtgctcg cgctggcggc ctgccagcag ggcgagaaga ccgtcctgtt gcgc
//
LOCUS       DAOOKS010000095         3890 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_28225_length_3890_cov_3.150978, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000095 DAOOKS010000000
VERSION     DAOOKS010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3890
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28225_length_3890_cov_3.150978"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="PK112_02355"
     CDS             <1..688
                     /locus_tag="PK112_02355"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPC10923.1"
                     /translation="LFTSDVTFRNFADLFASGRIGRYLFNLAFVALVVTAGNILFCFM
                     AAYVLARRPGLWTRVLFASVVVVLMIPAHILIVPLYVLMLKARLYDTYWALVLPWLVY
                     PIGIFLIKQYVEAIPPSMEEAARIDGAGELRILLAVVMPVCRPALAVLAIQAFFTNWN
                     SFLFPFILVSREELRTLPVGLAHYQGQQAIDWQHLMAGSAVAVVPILVLFIFLQRHIV
                     SGITAGGIKQ"
     gene            812..1414
                     /locus_tag="PK112_02360"
     CDS             812..1414
                     /locus_tag="PK112_02360"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPC10924.1"
                     /translation="MKVLMTTVLVVGLLLATGCGVNKEYVQQQIAESESRTGAQVGQL
                     QAGVDANEQQIQQLQALGQQLSEKTDLALNKASGFENYQVLWDNIVNFDFDQYELTEL
                     AKETLLEACEKMEQNPGSVATIAGHTDQTGSARYNLQLGQMRAEATKLFLADRCGAQP
                     YRMFIISYGKSKPAAMGDERNAASKNRRVQVTIWGIPQQQ"
     gene            complement(1528..3204)
                     /locus_tag="PK112_02365"
     CDS             complement(1528..3204)
                     /locus_tag="PK112_02365"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPC10925.1"
                     /translation="MTIAAVLRAAALAVLLAGCAEIAPPPGGDVDRTPPALLAVTPPN
                     GATNVPLSDRVVLRFSERVLAPQTGKAFFISPHLRAEPTVKWRGDRIEIIFPDSFQAN
                     QTYIIALSSAISDLRNNVFDSAGVIAFSTGATLDSGRVAGTVLGAGGSPQGGLLAGLY
                     DATLFTDSTVWDSLFPTYLTQTNQQGQFSFAYLPDRPYRLVVFQDRNRNERFNPARES
                     FALADRPVIVGGALRLSGLRLGLTTTDTTTAHVLSATFTADRLLRLRLDRPVATDHLS
                     ANPQDLALVSLADTTVRVTAQAILEQGEETSATLTAWLGSPDSGLYRVTAIYDPDRPP
                     MAYDSLRIAGAEDQTAPQLVAFAPAGPGFLRDLDLRLTVSEPLDTSAITPETFVLWRE
                     PDTMRVPLAWSWKDVFHLTFGKESLREGEGYRLAVTEFDLKDRAGNVAGDSLREYRFN
                     TLDVDSLGSITGQVRIGLPDREKDTVELTFKPVGGTGAFYWRGPQGGFTLAAPAGKYL
                     LSGYIDSNGDGALSLGSVDPWRTAETQSAYADTVQVRARFETAGIDFVLQ"
     gene            complement(3201..>3890)
                     /locus_tag="PK112_02370"
     CDS             complement(3201..>3890)
                     /locus_tag="PK112_02370"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPC10926.1"
                     /translation="SVFTALVASGENSPVNHEYLGRIALRQDDPPRAVEQFRRALALS
                     DSTWEPWVDLAAAYRAGHQRNEEIAAYREGLGQVRDTAGQAQLMMALGAAYEQTGQFD
                     SAVATFERLVALAPTFDAALNYLGYMLADSGQRLDYARELIERALQLKPNNAAYLDSY
                     GWVFYRLGRYEEARAQLERAVGLDSDPVMFDHLGDIYHALGQAEAAQHWWHKALELDP
                     DNATIRAKLDL"
BASE COUNT          656 a         1318 c         1232 g          684 t
ORIGIN      
        1 cctcttcacg tccgatgtga cattccgcaa tttcgccgac ctgttcgcct ccggccgcat
       61 cggccggtac ctcttcaatt tggctttcgt cgcgctggtg gtcacggccg gcaatatcct
      121 cttctgcttc atggcggcct acgtgctcgc ccgccgcccg gggctctgga cccgcgtgct
      181 ctttgcctcc gtcgtcgtcg ttctcatgat ccccgcccac attctcatcg tgccgcttta
      241 tgtgctcatg ctcaaggccc gcctctacga cacgtactgg gcgctggtcc tgccgtggct
      301 ggtgtatccg attggcatct tcctgatcaa gcagtacgtg gaggcgatcc cgccgtcgat
      361 ggaggaggcg gcccggatcg acggcgccgg ggagctgcgc atcctgctcg ccgtcgtcat
      421 gcccgtctgc cggccggccc tggcggtgct cgctatccag gctttcttca ccaactggaa
      481 ctccttcctc ttcccgttca tcctcgtgtc gcgggaggag ctgcgcaccc ttccggtggg
      541 tctcgcccac taccaggggc agcaggcgat cgattggcag cacctcatgg cgggttcggc
      601 ggttgcggtc gtgcccatcc tcgtgctttt catttttctg cagcgccaca tcgtctccgg
      661 aataactgcc ggcggtataa agcagtagcg gcgaaaccgg ggtcactttt ttcttgcctc
      721 ggcgccgcaa atggcggata ctgagggatc atgtgaactg accacgcaag cggatgattg
      781 aacctaaact cttgcgaaag gatgcagacg tatgaaagtt ctcatgacaa cggtcctggt
      841 cgtgggactg ctgctggcga ccggctgcgg cgtcaacaaa gaatacgtgc agcagcagat
      901 tgccgaatct gagagccgca cgggcgccca ggtcggccaa ctccaggccg gtgtcgacgc
      961 gaacgagcag cagattcagc agctccaggc gctcggccag caactctccg aaaagaccga
     1021 cctggctctg aataaagcgt ccggctttga gaactaccag gtactctggg acaacatcgt
     1081 gaacttcgac ttcgaccagt acgaactgac ggaactggcc aaagagaccc ttctggaagc
     1141 ttgcgagaag atggagcaga atcccggctc ggttgccacc atcgccggcc ataccgacca
     1201 gaccggctcc gcccgctaca acctgcagct tggtcaaatg cgcgctgaag ccaccaagct
     1261 gttcctggcc gaccgctgcg gggcccagcc ctaccgtatg ttcattatca gctacggcaa
     1321 gagcaagccg gccgcaatgg gcgatgagcg caacgccgct tcaaagaacc gccgcgtcca
     1381 ggtgaccatc tggggcatcc cccagcagca gtagcgacca gagtcttcag gatcacgcaa
     1441 aaggcggaac cgctcggtcc cgcctttcct atttcacccg cagtcttgcc ggccggccgc
     1501 gccgatcacc ggtccgcctg tcctgtgcta ctggaggaca aagtcgatgc cggcggtctc
     1561 gaagcgcgcc cgcacctgca ccgtatcggc gtaggcgctc tgggtctcgg ccgtccgcca
     1621 cggatcgacc gacccgagcg acagcgcgcc gtcgccgttg ctgtcgatgt accctgacag
     1681 gaggtacttg cccgccggcg ccgccagggt gaaacctccc tgcggcccgc gccagtagaa
     1741 cgcccccgtg ccgccgaccg gcttgaatgt cagctcgacc gtgtccttct cccggtccgg
     1801 caggccgatc cgcacctggc ccgtgatcga cccgagcgaa tcgacgtcga gggtgttgaa
     1861 acggtactcg cgcagcgagt cgcccgcgac gttgcccgcc cggtccttca gatcaaactc
     1921 ggtcaccgcc agccggtacc cctccccttc ccgcagcgac tccttgccga aagtcaggtg
     1981 gaacacatcc ttccacgacc atgccagcgg cacgcgcatt gtatcgggct cccgccagag
     2041 aacgaaggtt tcgggggtga tcgccgaggt gtcaagcggt tcgctgacgg tcagccgcag
     2101 gtcgagatcg cgaaggaacc cgggaccggc cggcgcgaac gccacaagct gcggcgccgt
     2161 ctggtcctcc gccccggcga tgcgcaggct gtcgtacgcc attggcgggc ggtccggatc
     2221 gtagatcgcc gtcacccggt acagcccgct gtccggcgag cccagccagg cggtgagcgt
     2281 ggccgacgtc tcctctccct gctccaggat tgcctgagcc gtgacccgca ccgttgtgtc
     2341 ggccagcgac acgagcgcga gatcctgcgg attggccgac aggtggtcag ttgcgacagg
     2401 ccggtccagc cgcagccgca ggagccggtc ggccgtgaag gtcgccgaga gcacatgcgc
     2461 cgtggtcgta tcggttgtgg tcaggcccag acggagaccg gagagccgca gcgccccgcc
     2521 cacgatcacc ggccggtcgg ccagtgcgaa cgactcgcgc gccgggttga accgctcgtt
     2581 gcggttgcga tcctgaaaaa cgacgaggcg gtaggggcgg tcgggaagat aggcgaagct
     2641 aaactgcccc tgctggttgg tctgtgtgag ataggtcgga aacaggctgt cccaaacggt
     2701 cgagtcggtg aacagcgtcg cgtcgtacag ccccgccagc agtcctccct ggggggatcc
     2761 gccggcgccg agcaccgtcc cggccacccg cccggagtcc agcgtcgctc ccgtggaaaa
     2821 ggcaatcacg ccggcgctgt cgaacacgtt gttccgcagg tcgctgatcg cgctgctcag
     2881 ggcgatgatg tacgtctgat tggcctggaa actgtcgggg aaaatgatct cgatgcggtc
     2941 ccccctccac ttcacggtcg gctccgcccg cagatggggg gagatgaaga acgccttgcc
     3001 ggtctggggt gcgagcaccc gctcggagaa ccggagaacc acccggtccg acagcgggac
     3061 attcgtggcg ccgttgggag gggtgacggc caggagcgcg ggcggcgtgc ggtcgacgtc
     3121 gccgcccggc ggcggagcga tctcggcgca gcccgccagg aggacggcca gggccgcggc
     3181 ccggaggacc gcagcgatcg tcacagatcc agtttcgctc gaatggtcgc gttgtcggga
     3241 tcgagttcga gtgctttgtg ccaccagtgc tgcgctgcct ccgcttggcc gagagcatga
     3301 tagatatcgc ccagatgatc gaacattacc gggtcgctgt caagtcctac cgcgcgctcg
     3361 agctgcgcgc gcgcctcttc gtaacggccg agccggtaga acacccatcc gtagctgtcc
     3421 agataggcgg cgttgttggg cttgagttgg agcgcccgct cgatcagttc ccgtgcgtaa
     3481 tccaaccgct gcccggagtc ggccagcatg taccccaggt agttcagggc ggcgtcgaac
     3541 gtcggggcca gcgccaccag gcgctcgaag gtggcgaccg cgctgtcgaa ctggccggtc
     3601 tgttcgtagg ccgcccccag cgccatcatc aactgggcct gcccggcggt gtcccgcacc
     3661 tgccccaatc cctcgcggta ggcggcgatc tcctcgttgc gctgatggcc ggcccggtag
     3721 gcggcggcca gatcaaccca tggttcccag gtcgaatccg acagagcgag cgcccggcgg
     3781 aactgctcga ccgcccgcgg cggatcatcc tggcgcagcg cgatccgccc caggtactcg
     3841 tggttgaccg ggctgttctc gccgctcgcc accagcgccg taaataccga
//
LOCUS       DAOOKS010000096         3889 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_28233_length_3889_cov_3.303599, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000096 DAOOKS010000000
VERSION     DAOOKS010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3889
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28233_length_3889_cov_3.303599"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..850
                     /gene="purM"
                     /locus_tag="PK112_02375"
     CDS             <1..850
                     /gene="purM"
                     /locus_tag="PK112_02375"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HPC10927.1"
                     /translation="DGVGTKLKLAFMTGKHDTVGEDLVNHCVNDILVHGARALFFLDY
                     IAVARLEPHVVADIVAGLARGCRNAGAALIGGETAELPDFYAPGEYDLAGFIVGAVEE
                     ARIINGASIRPGMVCLGLPSSGLHTNGFSLARKAAFEIAGRRPGDRIEELGTTVAEAL
                     LAVHKSYAPVVHPLLPQFPIHGMAHITGGGLPGNLNRVLPKDCDAWVRKGSWPVPPVF
                     DWLGRAGDLDREDMYSAFNMGIGFVVVVEAEQADQVVRTIERLGEKAYRIGEIRKGEG
                     KVRLTD"
     gene            997..2226
                     /gene="apgM"
                     /locus_tag="PK112_02380"
     CDS             997..2226
                     /gene="apgM"
                     /locus_tag="PK112_02380"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HPC10928.1"
                     /translation="MVKRVVLLVCDGLADRPIKEFGYKSPLEAARTPHLDRLAKESVC
                     GMVHTLGRGRVPGSDVAHLEIFGYPIEKYYAGRGPIEVCGLGMKLQGGDVALRGNFAT
                     VDKDWNIVDRRAGRINHVAPLCAAIDGIEIDGVKFLVHPGTGHRAGVIMRGEGLSDKI
                     IDADPHGPGEPVRTVTATDASPEARRTAEVLNRFLRKSYEALGGLDLNRQRAKEGKLP
                     ANILLVRGAGQHIHAPSFSERYGLKACAIAGGGLYKGVGSFVGMDVVEVPGATGLPDT
                     DIAAKFRAVLAKLAEYDFVFVHVKAADSLGEDGNAPGKKDFVEKIDAAAKILAEGLPE
                     DVLLVATADHTTPCEMKAHAADPVPIMFRGNNVRVDGVAAFGERACAQGGLGFMLGRE
                     IMPQVVNFLGKGHLEGA"
     gene            2816..>3889
                     /locus_tag="PK112_02385"
     CDS             2816..>3889
                     /locus_tag="PK112_02385"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HPC10929.1"
                     /translation="MSESRSYATIRAAGLLLAAAIGAALLASSVPAMVATPEEAARVS
                     QNWLTYMVYQQGNWAGEKAPQLLGSEEITFQGMVVGYHFRVAPSGYIVVPVLKELPPI
                     KAYSETDVLNVKNRQGMGGLLREVLYQRIAEYIKVYGSADAAQPATGDVFLDRSHGRE
                     WEMFLQSEASLTAELYSGRAGRQVTVGPLLASTWHQTGPYNSMCPVGDAACLDCADPG
                     EDPSPPSSDTAVVGCVATAAAQIMYFWNWPPSGTGSYSYTWDGDQTCGQPAGSTPAQT
                     LSANFSDAYDWGNMRDSYTGGESQAQRDAAAELCYEAAVAAGMDFGVCGSGTSTDTVL
                     TAFPMYFRYSAATIDKENRTDHTA"
BASE COUNT          685 a         1172 c         1428 g          604 t
ORIGIN      
        1 cgacggcgtg gggaccaagc tgaaactcgc gttcatgacc gggaaacatg acacggtcgg
       61 cgaggatctg gtcaatcact gcgtcaacga tatcctcgtc catggggcgc gggcgctgtt
      121 ctttctcgac tacatcgccg tggcgcgtct cgagccgcac gtggttgccg atatcgtggc
      181 cggcctggcg cggggctgcc gcaacgccgg ggcggcgctg atcggcgggg aaacggcgga
      241 gctgccggat ttctacgcgc cgggggagta cgacctcgcc gggttcatcg tcggcgccgt
      301 cgaggaggcg aggatcatca acggcgcctc cattcggccg gggatggttt gtctcgggct
      361 cccctcgagc ggcctgcaca ccaacgggtt cagcctcgcc cgcaaggcgg cgtttgagat
      421 cgccgggcgc cgcccgggcg accggatcga ggagctgggg accaccgtgg cggaagcgct
      481 cctggccgtg cacaaatcgt acgcgccggt cgtgcacccg ctgctgcccc agttccctat
      541 tcacggcatg gcccacatca ccgggggcgg cctgccgggg aatctcaacc gcgtcctgcc
      601 gaaagactgc gatgcctggg tgcggaaagg ttcctggccg gtgccgccgg tgttcgactg
      661 gctgggcaga gcaggcgacc tcgaccggga ggacatgtac tcggccttca atatggggat
      721 cgggttcgtc gtcgtggtgg aggcggagca ggcggaccaa gtcgtacgca cgatcgagcg
      781 gttgggagag aaagcctacc ggatcgggga gatccgaaag ggggagggga aggtgaggct
      841 cactgactga ttaagctccc tgcggctccg cgccgagcga ccgcatcgca gggccggggg
      901 cagggcgcgg gaaaggatcg cttccgggcg gctgagccat gacggaaaca caggatcagt
      961 tctgagacag cgacgtgaga cggagatatc aggaagatgg tcaagagagt ggtgctgctc
     1021 gtgtgcgacg gtctggcgga tcgtcccatt aaggagttcg gatacaagag ccctctggag
     1081 gcggcccgga cgccgcacct ggaccggctg gcgaaagagt cggtgtgcgg gatggtccac
     1141 acgctcgggc gcgggcgggt gccgggatcg gacgtggcgc acctggagat tttcggttac
     1201 ccgatcgaga agtactatgc cgggcggggg ccgatcgagg tgtgcgggct gggcatgaaa
     1261 ctccaggggg gagacgtggc gctgcggggg aattttgcga cggtcgacaa ggactggaat
     1321 atcgtggatc gccgcgccgg acggatcaac cacgtggcgc cgctgtgcgc ggcgatcgac
     1381 gggattgaga tcgacggcgt gaagttcctg gtgcatccgg gaacaggcca ccgggccgga
     1441 gtgatcatgc ggggcgaggg gctgtcggat aagattatcg acgccgaccc gcacgggccg
     1501 ggcgagccgg tgcgcacggt gaccgcaacc gacgccagcc cggaggcccg gcggacggcc
     1561 gaggtgctca acaggttcct ccgtaaatcg tacgaggcgc tcggcggact ggacctcaac
     1621 cggcagcgcg cgaaggaggg gaagctcccg gccaacatcc tcctcgtgcg cggcgcgggg
     1681 cagcacatcc acgccccgag tttctccgaa cgttacggtc ttaaagcctg cgcgatcgcc
     1741 ggcggcgggc tgtacaaggg tgtcgggtcg tttgtcggga tggatgtcgt cgaggtgccg
     1801 ggggcgaccg ggctgccgga cacggatatc gcggccaagt ttcgcgcggt cctggccaag
     1861 ctcgccgagt atgatttcgt gttcgtgcac gtgaaggcgg ccgattcgct cggcgaggac
     1921 ggcaacgcgc cggggaagaa agattttgtc gagaagatcg acgcggcggc gaagatcctc
     1981 gcggagggcc tgccggagga cgtgctgctg gtggcgacgg ccgaccacac cacgccgtgc
     2041 gagatgaagg cccacgcggc ggacccggtg ccgatcatgt ttcgcggaaa caacgtccgg
     2101 gtcgacggcg tcgcggcctt cggcgaacgg gcctgcgccc aagggggact gggtttcatg
     2161 ctcgggcggg aaatcatgcc gcaggtggtc aatttcctcg gcaaaggaca cctcgaagga
     2221 gcctgagccg ggtccgggcg cccggcgaag cagcggagaa ggagagaatc ggcgggctgc
     2281 gaccccggac ggtcccgcct ccctcccgac cggaacacct gccggagcgc cccgccatcg
     2341 caaatgtcct tgacaggcag tccccgctcg ccgcagatta ggaccatacg aacgcagagt
     2401 tgatcggcgc ggccgtgccg ggcgagacgg cattaccgtt cggctgcgcc gggagtcagg
     2461 gctgtgaggg tccccattac cggcagcagg tgctctttgg ccgatcgggc ggtccgcccg
     2521 cttgggcggc atgtcgctcc gaagtgtgcg ccgggtgtgg gtgaccgcgg gcgagcaaca
     2581 gtgaacacgc agggtgatta tcatcgaacg gcggcctgcg cgatgcggcg cgcggcggat
     2641 ttgaggcgcc gccgggcaat tccgcatcgc tgcactcgtc ggagaagcgg ccgatgccgg
     2701 tccccaaccg gcgaaggacc aagcacttac atacctgaac tgtttgaccc aactgaccgt
     2761 caatcgatat ggtttcccga ggcaaagccg tcgggggaca aagggaggtg ctatgatgtc
     2821 ggaatccagg tcgtatgcga ccatccgcgc ggccgggttg ctgctggcgg cggccatcgg
     2881 cgccgcactg ctggcgtcct cggtgccggc catggtggcg acaccggagg aagcggcccg
     2941 ggtcagccag aactggctca cctacatggt gtaccagcag ggcaactggg cgggcgaaaa
     3001 agcgccgcaa ctactgggga gcgaagaaat cacattccag ggtatggtgg taggctacca
     3061 tttccgcgtc gctccatccg ggtatatcgt ggtccctgtt ctgaaagagc tgccgccgat
     3121 caaggcgtac tccgagaccg acgtactcaa cgtgaagaat cggcagggga tggggggact
     3181 gctgcgcgag gtgctgtacc agcgcatcgc ggagtatatc aaagtgtacg ggagcgccga
     3241 cgcggcccag ccggccaccg gcgacgtctt cttggaccgc agtcacggac gggagtggga
     3301 gatgtttctg cagagcgagg cgagtctgac cgccgaactg tactcggggc gggccgggcg
     3361 gcaggtgacg gtcggtccgc tgctggcctc gacctggcac cagaccggac cgtacaacag
     3421 catgtgcccg gtcggggatg ccgcctgcct ggactgcgcc gacccgggtg aggacccctc
     3481 gcccccgtcg tcggacaccg ccgtcgtcgg ttgcgtggcg acggctgcgg cccagatcat
     3541 gtacttctgg aactggccgc cctcgggcac gggttcgtac agctatacct gggatggcga
     3601 tcagacgtgc gggcagccgg cgggcagcac gccggcccag acgctgtcgg ccaatttctc
     3661 cgatgcgtac gactggggga acatgcgcga cagctacacg gggggggaga gccaggccca
     3721 gagagacgcc gccgccgagc tctgctatga ggcggcggtc gcggcgggga tggatttcgg
     3781 cgtctgcggg tccggcacca gcaccgacac tgtcctgacc gccttcccca tgtactttcg
     3841 ctacagtgcg gccaccatcg acaaggagaa ccgaaccgat cacactgcg
//
LOCUS       DAOOKS010000097         3861 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_28514_length_3861_cov_2.849711, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000097 DAOOKS010000000
VERSION     DAOOKS010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3861
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28514_length_3861_cov_2.849711"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="PK112_02390"
     CDS             complement(<1..297)
                     /locus_tag="PK112_02390"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HPC10930.1"
                     /translation="MKIIVFTDTHANLPALRAFRRAIEREGYDLAVHTGDAVDIGPYP
                     AESLAELLSIPRVECVMGNHDAMFVHGIPDPLAGVIDGNEAEHCAWTARQLGEDF"
     gene            441..1820
                     /locus_tag="PK112_02395"
     CDS             441..1820
                     /locus_tag="PK112_02395"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HPC10931.1"
                     /translation="MEAFKYHVYICNQEKPEGVPCCRARGGEKLIDALRREVAAQGLG
                     DDVHITTCGSLGLCAWGPNMIVYPEGVWYTGVGAEDVPEIVREHFGRGRPVERLARRD
                     AAAVRAEIETNRAKMLAGLKARDAAGVLPDELMADFRGFQTSRMLLTAVELDLFTAVG
                     GSASAGEIAARLEVDERGAEQLLDALTAYGLLEKHGRRYANSAAARRYLAAGGPDDSR
                     LSLLHLVGLWNRWHTLTECIRKGTSVTYTEAKDRDPGWTEAFIAAMHKNAALRADQVV
                     GALGVAGFERMLDVGGGSGAYAISFAKANPRLRAEVFDLEPVTAIARRHIDRAGLAER
                     VTTRVGDLHTTEFGSPFDLVFISAICHMNSPEENRQLCHKAFAALRPGGTVAIQDFIL
                     NADKTGPFTAALFSLNMLVGTPAGASYSISEYTEWLTGAGFADLRVVALPGPTGLVVA
                     CRPEVGPHG"
     gene            1813..3237
                     /locus_tag="PK112_02400"
     CDS             1813..3237
                     /locus_tag="PK112_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HPC10932.1"
                     /translation="MDDAEFRRRAHELVDWMADYLANVRAYPVKSPAAPGDIRRQLPD
                     LPPETPEAFDDIMADFRRIIMPGITHWQHPRFMGYFPANSSPASVLAEMLTATLGVQG
                     MLWQTSPAAAELEEQMMLWLGAMIGIPAGWDGVIQDTASTATLTSILTAREQASDYGV
                     NQTGLQDRPRYAVYGSSETHSSIEKAVKIAGLGRENLRKVAVDAGFALRPEVLEEAVC
                     RDEDSGIRPLCAVATLGTTGSTAVDPLRRIGEICRRHRLWLHVDAAYAGTALVLPEMR
                     WMIDGIEEADTFVFNPHKWMFTNFDCSAYFVKDRGALIRTFEILPEYLKTRETAAVNN
                     YRDWGIPLGRRFRALKLWFVIRTYGLSGIREKLRLQIGLARGVADRIMESDDFELLAP
                     APLNVLCFRYRPTGAADSEALNRLNERLLEEVNRTGRVFITHTKLNGAFTLRMVIGQT
                     QVTADDVDLAWETIAGTARALGRG"
     gene            3270..>3861
                     /locus_tag="PK112_02405"
     CDS             3270..>3861
                     /locus_tag="PK112_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10933.1"
                     /translation="MADQSFPENAPEAPAVSAIPLPLAPVADAERVPSLDVLRGFAVL
                     GILAINIQQMGLPQAALLNPHLQGEAGLLHTIAWGGVIVLFLQKFMTIFSLLFGAGLV
                     VMTQRAQAAGGDAASLHYRRMGWLLVIGLLHAYLLWWGDILFSYALCGMLVYLLRRLS
                     PRRLLIIGSLVFLAPAPFAAGFSGLIVTLREHPDAWA"
BASE COUNT          585 a         1373 c         1255 g          648 t
ORIGIN      
        1 gaagtcctcg cccagctggc gggcggtcca ggcgcagtgt tccgcctcgt tgccgtcgat
       61 cacgcccgcg agcgggtcgg gaatgccgtg gacaaacatc gcatcgtggt tgcccatgac
      121 gcattccacc cgcgggatgc tcagcagttc ggccagactc tcggccgggt acgggccgat
      181 atccaccgcg tcgccggtgt gcacggccag atcgtagccc tctctctcga tcgcccggcg
      241 gaaggcccgc agcgccggca ggttggcgtg ggtatcagtg aacacgatga tcttcatggc
      301 cgctccaccg acattctacg gcagcccggc ccgccggaca agcaattcat cggccctccc
      361 tcttgtcccc cgcccgtttt cctcgtacct tgcagcaaca cccggcacag tgcgccgtcc
      421 tgtggagaaa tgagccgcct atggaagcct tcaagtacca cgtctacatc tgcaatcagg
      481 agaagcccga gggcgtgccg tgctgccgcg cccgcggggg cgaaaaactg atcgacgctc
      541 ttcgccgcga ggtggcggcg caagggctgg gcgacgatgt gcacattacg acctgcggct
      601 cgctcggcct gtgcgcctgg ggccccaaca tgatcgtcta tcccgagggg gtctggtaca
      661 ccggcgtagg ggccgaggac gttcccgaga ttgtccgcga gcacttcggg cgcggccgtc
      721 cggtcgagcg actggcgcgg cgcgacgccg ccgccgtgcg cgccgaaatc gaaaccaacc
      781 gggcgaaaat gctcgccggg cttaaggccc gcgacgcggc cggcgtcctc ccggacgagc
      841 tgatggccga tttccggggc tttcagacca gccgtatgct cctgaccgcc gtcgagctcg
      901 acctcttcac ggccgtgggc ggctccgcct cggccggcga gatcgccgcc cggctcgagg
      961 tcgatgagcg gggggcggag cagttgctcg acgctctcac cgcctacggc ctcctggaga
     1021 agcacgggcg ccgctacgcc aactccgccg ccgcgcgccg ctacctggcg gcgggcgggc
     1081 ccgatgattc ccggttgagc ctcctgcacc tcgtcgggct gtggaaccgg tggcacacgc
     1141 tgaccgagtg catccgcaag ggaacgtcgg tgacctacac ggaggcgaaa gatcgcgacc
     1201 ccggctggac cgaggctttc atcgccgcca tgcacaagaa tgcggcgctg cgcgccgacc
     1261 aggtggtggg cgcgctgggg gtggcggggt tcgagcggat gctcgatgtc ggcggcggct
     1321 cgggggccta cgcgatttca ttcgccaagg ccaatccgcg cctgcgcgcc gaggtgttcg
     1381 acctcgaacc ggtcaccgca atcgcccggc ggcacatcga ccgggccggc ctcgccgagc
     1441 gcgtcaccac gcgcgtcggc gacctgcaca ccaccgagtt cggctccccc ttcgatctcg
     1501 tgttcatctc ggccatctgc cacatgaaca gcccggagga gaaccggcag ttgtgccaca
     1561 aggctttcgc cgccctccgc cccggcggca ccgtcgccat ccaggatttc attctcaacg
     1621 ccgacaagac cggcccgttc acggcggcgc tcttttcgct caacatgctc gtcggcacac
     1681 ccgcgggagc ttcctacagc atcagcgagt ataccgagtg gctgaccggc gccggttttg
     1741 ccgacctgcg cgtcgtggcc ctgccgggtc cgaccgggct cgtggtcgcc tgccgtccgg
     1801 aggtcggtcc tcatggatga cgccgaattc cgccgccgcg cccacgaact ggtcgactgg
     1861 atggccgact acctcgccaa cgtgcgcgcc tatccggtca agtcccccgc cgccccgggc
     1921 gacatccggc ggcagctgcc cgaccttcct cccgagaccc cggaggcgtt cgacgatatc
     1981 atggccgatt tccgacggat catcatgccg ggcatcacgc actggcagca cccccggttc
     2041 atggggtatt ttcccgccaa cagcagcccg gcctcggtgc tggccgagat gctcaccgcc
     2101 actctcggcg tgcaggggat gctctggcag acttcgccgg ccgccgccga actcgaggaa
     2161 cagatgatgc tatggctggg cgccatgatc ggcatcccgg ccggctggga tggcgtcatc
     2221 caggataccg cctccaccgc caccctcacc tcgatcctca ccgcccgcga acaggcgtcc
     2281 gactacggtg tcaaccagac cggcctccag gaccggccgc gctatgccgt gtacggctcg
     2341 tcggaaacgc actcgtcgat cgagaaggcg gtcaagatcg cggggctcgg cagggagaac
     2401 ctccgcaaag tggcggtcga tgcggggttt gcgctgcggc cggaggtcct cgaagaggcg
     2461 gtctgccgcg atgaggacag cggaattcgc ccgctctgcg cggtggccac gctcggcacg
     2521 accggctcca cggcggtcga tcccctccgc cggatcggtg agatctgccg ccgccaccgc
     2581 ctctggctcc atgtcgacgc cgcctatgcc gggacggccc tcgtgctccc ggagatgcgg
     2641 tggatgatcg acgggattga ggaggccgac acgttcgtgt tcaacccgca caagtggatg
     2701 ttcaccaact tcgactgctc cgcgtacttc gtgaaagacc ggggcgctct catccggacg
     2761 ttcgagatcc tcccggagta tctgaagacg cgcgagacgg cggcggtcaa caactaccgc
     2821 gactggggga tcccgctggg ccggcgcttc cgcgcgctga agttgtggtt cgtcatccgc
     2881 acctatggcc tgtccgggat acgggaaaaa ctccgcctcc agatcgggct cgcccgcggt
     2941 gtggcggacc gcatcatgga atcggacgat ttcgaactgc tcgccccggc gccgctcaac
     3001 gtcctctgtt tccgctaccg gccgaccggg gcggccgaca gcgaggcgct caaccgactg
     3061 aacgagcgcc tgctggagga ggtcaaccgc acgggccggg tgttcatcac ccacacgaaa
     3121 ctcaacgggg ccttcacgct ccgcatggtg atcggccaga cgcaggtgac cgccgatgat
     3181 gtcgacctcg cctgggagac gatcgccggc acggcgcggg ccctcggccg cggttaggtg
     3241 agacgcctga cccggcgaag gagatgagca tggctgacca gtccttcccc gagaacgccc
     3301 ccgaggcccc ggctgtgtcg gccattccgc tgccgctggc gccggtggcc gatgcggaac
     3361 gtgtgccctc gctggatgtg ctccgcgggt tcgccgtact cggcattctc gcgatcaaca
     3421 tccagcagat gggcctcccg caggcggcgc tcctgaaccc gcaccttcag ggagaggccg
     3481 gcctgctgca cactatcgcc tggggagggg tgatagttct tttcctgcag aagttcatga
     3541 cgattttcag cctgctcttc ggcgcgggcc tggtggtcat gacccagcgg gcccaggccg
     3601 ccggaggcga cgccgcctcc ctccactacc gccggatggg ctggttgctg gtgatcggcc
     3661 tgctccacgc ctacctgctc tggtggggag acatcctctt ctcctacgcc ctgtgcggca
     3721 tgctcgtcta cctcctgcgg cggttgtcgc cgcgccggct gctgattatc ggctctctcg
     3781 tcttcctggc gccagcgccg tttgcggccg gcttctccgg gctcatcgtg acgctgcgcg
     3841 agcaccccga cgcgtgggcc t
//
LOCUS       DAOOKS010000098         3853 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_28597_length_3853_cov_2.767773, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000098 DAOOKS010000000
VERSION     DAOOKS010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3853
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28597_length_3853_cov_2.767773"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..861)
                     /locus_tag="PK112_02410"
     CDS             complement(<1..861)
                     /locus_tag="PK112_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016547608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10934.1"
                     /translation="MFYSPVITNAAEAADNKKLHLFRYVEDPEQYKLAAYERHGGYRA
                     WRKVLAGMKPDEVIEVVKKSGLRGRGGAGFPTGMKWGFIPKDSPKPRYLCCNADESEP
                     GTCKDRVLMERDPHAIIEGMAIAAYAIGCHIMFIYIRGEFGHPIARMEAAMKEAYAAG
                     HLGRNIHGSGYDLDDLVAVPWTTAQKKIRGRYSTWLDDVLCSAATMEDVDHAVADVRS
                     LLRQRHGIGPGDEEDFNIRRPDEVIKAQIEASQTLERLLIALASISLLVGGIGIMNIM
                     LASVTARTNEI"
     gene            complement(861..1337)
                     /gene="nuoE"
                     /locus_tag="PK112_02415"
     CDS             complement(861..1337)
                     /gene="nuoE"
                     /locus_tag="PK112_02415"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HPC10935.1"
                     /translation="MILSDESVRKIKEKVPLYPRRKSSVLPALTIAYQQLGYVDDDIY
                     REIARVIGVPYVEVAEAASFYTMFPKAPRGKYLIQVCHNLSCALLGADSLIAYLEKSL
                     GIRKGETTPDKLFTLISVECLGSCATAPMMQINNDFYENLTPAKVDQILNDLRSQA"
     gene            complement(1334..2506)
                     /gene="nuoD"
                     /locus_tag="PK112_02420"
     CDS             complement(1334..2506)
                     /gene="nuoD"
                     /locus_tag="PK112_02420"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005997658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HPC10936.1"
                     /translation="MAEARTVTINMGPQHPSTHGVLRVILELDGETVVKARPVVGYLH
                     TGIEKTMESKLYYKALPCTDRMDYLAPMSNNLGYSLAVEKLLGIEVPEKVKWARVILA
                     ELTRINSHLVWLGTHALDLGAMSMLLYAFRERELILDVYEACGGQRMMTSYIRIGGLA
                     HDLPKDFDRKVRDIIATLTARLEDYEGLLTRNEIFINRTKGVAVISAEDALNWSLSGP
                     MLRGSGVARDLRKDEPYSGYENFDFQIALGHNGDAYDRYLLRMEEIRQSLRICRQGID
                     GLPEGPYRAHVPGVVLPPKEDVLYKMESMIFHFKIITEGFAAPLGSVYQAIESPKGEL
                     GFYVVGDGTTMPRRIRVRPPSLINLASLPRLVEGRLFSDVICAIGSIDIVLGEVDR"
     gene            complement(2506..3045)
                     /locus_tag="PK112_02425"
     CDS             complement(2506..3045)
                     /locus_tag="PK112_02425"
                     /inference="COORDINATES: protein motif:HMM:TIGR01961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HPC10937.1"
                     /translation="MKSETSEGNDARIRRFLRERFGEAIRHEEVFRGDLQFTVDPEKI
                     YDICEALMEAGGLDVRFLADITCVDWHGHSEAVHGRFEVVYNLCSLAHRSRFFLKVRL
                     PETNPHVRSLVDLWHGANWMEREVWDLFGVTFDGHPDLTRLLTPDDFEGHPLRKDYPL
                     VYEQPKFSWNENDPPEVIR"
     gene            complement(3146..3598)
                     /locus_tag="PK112_02430"
                     /pseudo
     CDS             complement(3146..3598)
                     /locus_tag="PK112_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006133126.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
     gene            complement(3589..>3853)
                     /locus_tag="PK112_02435"
     CDS             complement(3589..>3853)
                     /locus_tag="PK112_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010958235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HPC10938.1"
                     /translation="RTVLGRKTDPYECGVEPVGTTRNPVPVKFYLIAISFILFDIELI
                     FLLPWAVVARDLGSYGFWAMAVFVAIIVVGYLYELRRGALKWD"
BASE COUNT          650 a         1200 c         1279 g          724 t
ORIGIN      
        1 gatctcgttc gtccgggcgg tgaccgaggc gagcatgatg ttcatgatcc cgatcccccc
       61 gacgaggagc gagatggagg cgagcgcgat gagcaaccgc tcgagggtct ggctcgcctc
      121 gatctgcgcc ttgatgacct cgtcggggcg ccggatgttg aagtcctcct cgtcccctgg
      181 accgatcccg tggcgctgcc gcagcagcga gcgcacgtcg gccacggcgt ggtccacgtc
      241 ctccatcgtc gcggccgagc agaggacgtc gtcgagccac gtcgagtagc gcccccggat
      301 cttcttctgg gcggtcgtcc aggggacggc gacgagatcg tcgaggtcgt agccggagcc
      361 gtggatattc cggccgaggt ggccggccgc gtaggcctcc ttcatcgccg cttccatgcg
      421 cgcgatcggg tggccgaatt ccccccggat atagatgaac atgatgtggc agccgatggc
      481 gtaggcggcg atcgccatgc cttcaatgat ggcgtggggg tcgcgctcca tgagcacgcg
      541 gtccttgcag gtgcccggct cggactcgtc ggcgttgcag cagaggtagc gcggtttggg
      601 ggagtccttg gggatgaatc cccacttcat gccggtgggg aagccggcgc cgccccgccc
      661 gcgcagcccg gacttcttga ccacttcgat cacctcgtcc ggcttcatgc cggcgaggac
      721 tttccgccag gcccggtagc cgccgtgccg ctcgtaggcc gccagcttgt actgctcagg
      781 atcctcgacg taccggaaca agtggagctt cttgttgtcg gcggcttcgg ccgcgttggt
      841 gatgaccggc gaatagaaca tcaggcctgg gacctcaaat cattcaggat ctgatcgacc
      901 ttcgcaggcg tcagattctc atagaagtcg ttgttgatct gcatcatggg cgcggttgcg
      961 cacgacccga gacactcgac cgagatgagc gtgaacagct tgtccggcgt cgtctcgcct
     1021 ttcctgatcc cgaggctctt ctcgaggtag gcgatcagcg agtcggcgcc gagcagggcg
     1081 caggagaggt tgtggcacac ctggatcagg tacttcccgc gcggcgcttt cggaaacata
     1141 gtatagaatg acgcggcctc ggccacctcg acatagggca cgccgatgac gcgggcgatc
     1201 tcccggtaga tgtcgtcgtc gacgtacccg agctgctggt aggcgatcgt cagcgcgggg
     1261 agcacggacg atttccggcg cgggtagagg ggcactttct cctttatctt tctgactgac
     1321 tcgtccgaca gaatcatcgg tccacctctc cgagcacgat gtcgatcgag ccgatggcgc
     1381 agatgacatc ggaaaacagc cggccctcga ccagtcgggg aaggctggcg agattgatga
     1441 gcgagggcgg ccggacgcgg atccggcggg gcatcgtcgt cccgtcgccg accacgtaga
     1501 agccgagctc gcccttgggc gactcgattg cctggtacac ggagccgagg ggagccgcga
     1561 aaccctcggt gatgatcttg aagtgaaaga tcatcgattc catcttgtag aggacgtcct
     1621 ccttgggcgg gagcacgacg cccgggacgt gggcccggta gggaccctcg gggaggccgt
     1681 cgatcccctg gcggcagata cggagcgact gccggatttc ttccatgcgg agcaggtagc
     1741 ggtcgtaggc gtcgccgtta tgccccaggg cgatctggaa atcgaaattc tcgtagccgg
     1801 agtagggctc gtccttgcgc aggtcgcggg cgacgccgct gccgcgcagc atcggacccg
     1861 agagcgacca gttgagggcg tcctcggccg aaatgaccgc cacccccttg gtgcggttga
     1921 tgaagatctc gttgcgggtg agcaggccct cgtagtcctc caggcgcgcc gtgagggtgg
     1981 cgatgatatc gcggactttg cggtcgaaat ccttgggcag gtcgtgggca aggccgccga
     2041 tgcggatgta gctggtcatc atgcgctggc cgccgcaggc ctcgtacacg tcgaggatca
     2101 gctcgcgttc gcggaaggca tacaggagca tcgacatggc gccgaggtcg agggcgtggg
     2161 tgcccagcca gacgaggtgc gagttgatcc gcgtgagttc ggccagaatc acgcgcgccc
     2221 acttgacttt ctcggggacc tcgatcccca ggagcttttc gaccgcgagg ctgtagccga
     2281 ggttgttcga catcggggcg aggtagtcca tccggtcggt gcacgggagc gccttgtagt
     2341 acagcttcga ctccatcgtc ttctcgatcc cggtgtggag atatccgacc acgggccggg
     2401 ccttgacaac cgtctccccg tccagctcca ggatgacgcg cagcacgccg tgggtcgagg
     2461 ggtgctgcgg ccccatgttg atggtcacgg tccgggcttc cgccattacc tgatcacctc
     2521 gggcgggtcg ttctcgttcc agctgaattt cggctgctcg tagaccagcg ggtagtcctt
     2581 gcgcagcggg tgcccctcga aatcgtcggg cgtcagcagc cgcgtcaggt ccgggtggcc
     2641 gtcgaacgtc accccgaaca ggtcccacac ctcgcgctcc atccagttgg cgccgtgcca
     2701 gaggtcgacg agcgaccgca cgtgcgggtt cgtctcgggg agacgcactt tcaggaagaa
     2761 gcggctgcgg tgggccaatg agcagaggtt gtagacgacc tcgaaccggc cgtggacggc
     2821 ctcggagtgg ccgtgccagt cgacgcaggt gatgtcggcg aggaagcgga catcgagtcc
     2881 gcccgcctcc atgagggctt cgcagatgtc gtagatcttt tccggatcga ccgtgaactg
     2941 cagatcgccg cggaacacct cctcgtggcg gatggcctcg ccgaagcgct cccggaggaa
     3001 gcggcggatt cgggcgtcgt ttccctcgct tgtttcactt ttcacaatcg ccggttcgtg
     3061 tgtttgcgcc atcgccgttg cctgtcgcct gcggtcagac cgccctgcgg ccccactctt
     3121 cccgggcgtc tttgagggat tccttttcga ttttctcgaa cagcttgagg atgccgtcca
     3181 tgagctgttc cggccgcggc gggcagccgg ggacatagat gtcaaccggg atgatgcggt
     3241 cgactccctg gagaatcgcg tagttgttga acacgccgcc gcacgaggcg caggcgccca
     3301 tggagatcac ccacttcggg ttgggcatct gctcgtagag ggtccggacc accggggcca
     3361 ttttgttgga cacgcgtccg gctacgatca tgaggtcggc ctggcgcggc gagggccgcg
     3421 tgacctccat cccgaaccgg gcgaggtcgt tgtgcgacgc aaaggtcgag atcatctcaa
     3481 tcgcgcaaca cgccagcccg aacccgagcg gccacatcga gcgcttgcgc gcccagttga
     3541 ccatcttgtc cagcgtcgtc aggattatcg aatcggggat tttctcttct agtcccactt
     3601 cagcgctccc cgcctcagtt catacaggta gcccaccacg atgatcgcca cgaacaccgc
     3661 catcgcccag aatccgtatg acccaaggtc gcgggcgacg accgcccagg ggagcaggaa
     3721 aatcagctcg atatcgaaca gaatgaagga gatggcgatc agatagaact tgaccggcac
     3781 cgggttcctg gtcgtcccga ccggctccac cccgcactcg taggggtcgg tcttccggcc
     3841 caaaaccgtc cgg
//
LOCUS       DAOOKS010000099         3849 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_28628_length_3849_cov_2.788877, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000099 DAOOKS010000000
VERSION     DAOOKS010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28628_length_3849_cov_2.788877"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="PK112_02440"
     CDS             <1..693
                     /locus_tag="PK112_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPC10939.1"
                     /translation="GLVGGGAVPKPGEVSLAHHGVLFLDELPEFKKDILEMLRQPMED
                     NEVTISRASTTITYPAGFMLVAAMNPCPCGYFGDPSHECNCSSSEIQRYMARISGPLM
                     DRIDIHITVPSVNFKDLSSETCGEKSTVIRARVNRARQRQIARFEEEKRIFCNAHMES
                     KDIRKYCLIDDKSQALLATAITRQGLSARAYDRILKVARTIADLGDSDHIDLGHIAEA
                     IHYRTLDRQLWM"
     gene            866..3553
                     /locus_tag="PK112_02445"
     CDS             866..3553
                     /locus_tag="PK112_02445"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPC10940.1"
                     /translation="MDRHKLDRLFDPRRIALIGVTINPNSVGGKVLANLVAGGYRGVV
                     YPVNPDSEAVMGIPCYPSVAALPRTPDLAVICAPAEKVPAAVLECGRAGILGIIILSA
                     GFKETGPEGAALEQEIRAARRQFPGMRIIGPNCLGLIVPSRNLNISFAGGMPRAGNVA
                     FLSQSGALCTSVLDWAMEGRIGFSHFVSIGNTIDVDFGDLIDYFGADDSTRSIILYIE
                     SIARAPRFMTAARAFARTKPIIAFKAGRFPESAQVAASHTGALASEDAVYDAAFQRIG
                     LARVYHLANIFDCVQLIGRNRIPTGPNLGIVTNAGGPGVIAADALIAAKGSLARLSDD
                     TLAQLNAALPSSWSHGNPVDILGDATSKRLEKAAQIVLADPAVEALLVILTPQAMTNV
                     TAAARVVGKLAESTAKPVLAAWLGGASMREGMQILTESGVATYRTPEQAVGAFMTLVD
                     YGRNLQTLYETPKDLPVEFELDRARLRADFVARHCGPHPILSEAVSKDLLEGYGIPTA
                     RPRPAADADEAVRIAGELGYPVVIKILSPDITHKTDVGGVALGLEDDAMVRSAFAHMM
                     LAVGEKAPRARIEGVTVQPMIDPRDSLEMILGIKKDATFGTVMMAGMGGVAAELLGDR
                     SLGFPPLNERLARRMLESLKIWPLLQGYRGRPRANVDRLLEVLIRLSYLAADFPEIAE
                     LDINPLLVSPRDVVALDARIDIDPALVGRSSEPYSHLALRPYPEKYVARPTLRDGTTV
                     TLRPIKPEDEPLWLAFLASCSKESIYSRFRYFFHWNSHDVATRFCYIDYNREIAIVAE
                     IVEGGERRIIGIGRLIADPDHEEVEYAVLIADAWQKKELGSLLTDYCMEVASRWKLKR
                     IVAQTTTDNRPMISVFEKRGFAIAFDETGTGVRVVKELA"
     gene            complement(3654..>3849)
                     /locus_tag="PK112_02450"
     CDS             complement(3654..>3849)
                     /locus_tag="PK112_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10941.1"
                     /translation="DAFYTASTLVMPTKKAGIQAIDPNSGTLKWSIEAEKSGLFAASE
                     SLTHIALATDKELRVVELKM"
BASE COUNT          634 a         1393 c         1177 g          645 t
ORIGIN      
        1 ggtctcgtgg gcgggggagc cgtccccaaa ccgggcgaag tctctctcgc tcaccacggc
       61 gtgctgtttc tcgatgaact ccccgaattc aagaaagaca tcctcgaaat gctgcgccag
      121 ccgatggagg acaacgaggt gactatctcg cgtgcgagca ccacgatcac ctacccggcc
      181 gggttcatgc tcgtggccgc catgaacccc tgcccctgcg gctatttcgg cgacccgagc
      241 cacgagtgca actgctcctc gagcgagatt cagcgctaca tggcgcgcat ctcgggaccg
      301 ctcatggacc gcatcgacat ccacatcacc gtcccgtcgg tgaatttcaa agacctctcc
      361 tcggagacct gcggcgagaa atcgactgtc atccgggccc gggtcaaccg cgcccgccag
      421 cgccagattg cccggttcga ggaggagaaa cggatcttct gcaacgccca catggagtcg
      481 aaggatatcc ggaagtactg cctgatcgac gacaagtcgc aggcgctctt ggcgaccgcg
      541 atcacgcgcc aggggctgtc ggcccgggcc tatgaccgca ttctgaaagt cgcgcggacg
      601 atcgccgatc ttggcgacag cgaccacatc gaccttggcc acatcgcgga ggcgattcac
      661 taccggaccc tcgaccggca gttgtggatg tgaggggcaa gtgaaggcgc acgagcgggg
      721 cgcggcgtcc gccggccgca ccctgtcagg gggcagtcgc ggcgctgtcg catcagatgc
      781 ggcgcccggc ccccctcccc caaaaattcc cccacgccgc tccgaaatgt cctacattat
      841 ccccgttgcc gtgcaggaga tgacaatgga cagacataaa ctcgaccgcc tctttgaccc
      901 ccgccgcatc gccctcatcg gcgtcacgat caaccccaat tccgtgggcg gcaaagtcct
      961 cgccaacctc gtcgccggcg gttaccgcgg cgtggtctat cccgtgaacc cggactccga
     1021 ggcggtcatg ggcatcccgt gctacccctc ggtcgcggcg ctcccgcgca cacccgacct
     1081 cgcggtcatt tgcgcgccgg cggagaaagt ccccgcggcc gtgctcgaat gcggccgggc
     1141 cggcatcctc ggcatcatca tcctctccgc cggtttcaag gagaccggcc ccgagggggc
     1201 ggcgctggag caggagatcc gggccgcccg gcggcagttt cccggcatgc gcatcatcgg
     1261 tcccaactgc ctcggcctca ttgtcccctc gcgcaacctg aacatcagtt tcgccggcgg
     1321 gatgccccgg gccggcaacg tggctttcct ctcgcagtcg ggcgcgctgt gcacctcggt
     1381 gctcgactgg gccatggagg ggcggatcgg tttctcccac ttcgtgtcga tcggcaacac
     1441 gatcgacgtt gatttcggcg acctcattga ttatttcggc gccgacgatt cgacccgctc
     1501 gatcattttg tacattgagt cgattgcgcg ggccccgcgc ttcatgaccg ccgcgcgcgc
     1561 gttcgcgcgc acgaaaccga tcattgcctt caaagccggc cgttttccgg aatcggcgca
     1621 ggtcgcggcc tcgcacaccg gggcgctcgc ctcggaggac gccgtgtacg acgccgcctt
     1681 tcagcgcatc ggtctcgccc gcgtctacca cctggccaac attttcgact gcgtgcagct
     1741 catcgggcgc aaccgcattc cgaccggccc caacctgggc atcgtcacca acgccggcgg
     1801 cccgggcgtg atcgccgccg acgccctcat cgcagccaag gggtcgctcg cccggctctc
     1861 cgacgacacc ctggcgcagc tcaacgccgc gctcccctcc tcgtggtcgc acggcaaccc
     1921 ggtcgacatc ctcggcgacg cgacctcgaa acggctcgag aaggccgccc agattgtgct
     1981 cgccgatccg gcggtggagg cgctgctggt cattctcacg ccgcaggcga tgaccaacgt
     2041 cacggccgcc gcccgggtgg tcggcaaact cgcggagagc acggccaagc cggttctcgc
     2101 cgcctggctc ggcggcgcct ccatgcgcga ggggatgcag attctcaccg agagcggcgt
     2161 cgccacctac cgcacgcccg agcaggcggt gggggccttc atgaccctcg tcgactacgg
     2221 ccgcaacctg cagacgctgt acgagacccc gaaagatctc ccggtcgaat tcgagctcga
     2281 ccgggcgcgg ctgcgcgccg atttcgtcgc ccgccactgc ggccctcatc cgatcctgtc
     2341 cgaggccgtc tcgaaagacc tcctcgaggg ttacggcatt cccacggcgc gtccgcggcc
     2401 ggccgccgac gccgacgagg cggtgcggat cgccggcgaa ctcggctacc ccgtcgtcat
     2461 caagatactc tcccccgaca tcacgcacaa aaccgacgtc ggcggggtgg cgctcgggct
     2521 cgaggacgac gccatggtgc gcagcgcgtt cgcccacatg atgctcgcag tgggggagaa
     2581 ggcgccgcgg gcgcgcatcg agggggtcac agtccagccg atgatcgacc cgcgcgacag
     2641 tctcgagatg attctcggga tcaagaagga tgcgacgttc gggacggtga tgatggcggg
     2701 gatgggcggg gtggcggccg agctgctggg tgaccggagt cttggctttc cgccgctcaa
     2761 cgagcggctc gcccgccgca tgctcgaatc cctcaagatc tggccgctcc tccagggcta
     2821 ccgcggccgc cccagggcca atgttgaccg ccttctcgaa gtcctcatcc gcctgtcgta
     2881 cctcgcggcc gatttccccg agatcgccga actcgacatc aacccgctgc tggtcagccc
     2941 gcgcgacgtg gtcgcgctcg acgcccgcat cgacatcgac cccgcgctcg tcggccggtc
     3001 ctccgagccc tattcccacc tcgccctgcg cccctacccg gagaagtacg tcgcgcgccc
     3061 cacgctccgc gacggcacga cggttactct ccgcccgatc aagcccgagg acgaaccgct
     3121 ctggctggct ttcctcgcga gctgttcgaa agagtcgatc tactcccgct tccgctactt
     3181 cttccactgg aattcgcacg acgtcgccac ccgcttctgc tacatcgact acaaccgcga
     3241 gatcgccatt gtcgccgaaa tcgtcgaggg cggggagcgc cggatcatcg gcatcggccg
     3301 tctcatcgcc gaccccgatc acgaggaggt ggagtacgcc gtgcttatcg ccgacgcctg
     3361 gcagaagaag gagctcggca gcctcctgac ggactactgc atggaggttg cctcgcgctg
     3421 gaagctgaag cggattgtcg cccagaccac gaccgacaac cggccgatga tttcggtgtt
     3481 cgagaagcgc ggtttcgcca tcgctttcga cgaaaccggc accggcgtgc gagtggtgaa
     3541 ggagctggcc tagcgagcgg gagcaggatg cccggcccgc cgcaatcgcc gggcgcggcg
     3601 aagcgcgcgg tgcggccggc gcgggcatcc cgccttgtct ctctcgcccc tcctcacatt
     3661 ttgagctcca ccaccctcag ctctttgtcg gtcgccagcg cgatgtgcgt cagcgactcc
     3721 gaggccgcaa acagccccga cttctctgcc tcaatgctcc acttgagcgt cccggagttc
     3781 gggtcaatcg cctgaatccc cgccttcttg gtgggcatca cgagcgtcga ggccgtgtag
     3841 aaagcgtcc
//
LOCUS       DAOOKS010000100         3848 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_28639_length_3848_cov_3.180332, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000100 DAOOKS010000000
VERSION     DAOOKS010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28639_length_3848_cov_3.180332"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1667
                     /locus_tag="PK112_02455"
     CDS             <1..1667
                     /locus_tag="PK112_02455"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HPC10942.1"
                     /translation="TIERLTDLVTMPENSPGGSDHQSFIDQGFPVSNSIEGTFNYPGW
                     HTNLDLTSEMNFPFFTKVVKAGLAALAIVANSARPTEIERLVDMGDGQSIEVIWKSCD
                     PSYRYVVRWGTASGVYTDSAVVPPGGCSYVVTGLTEGQPYFFTAEGSVEDSYPSIYST
                     EDSLTPLVVPRRPSALVADPALQAIALSWRPNTEGDLTGYKIYRKIEDYDYQLLAGGV
                     TGQSYTDSAIAPHSLFTYRISAVDGDAHESELSPESGNYAATFDGGILIVDETSQHPS
                     VPTQAGQVAFWDTILGPTPYSLEVTDFAGAALRRGIAGQYSSIFWFDDDFTNHFLKTS
                     QDSLTWYAGYTGNILVAGLRTVQYWTSSVIYPDDFVYTDFGLASWTENSAFDFAGAHG
                     QNGWPSLTVRPNVFNNLPYVPSLQLAPGATLLYTYNSATDNPAREGQPVGILKTTPHG
                     ARIVLAFPVMFLTDSSATQLMNFVKAQFGESGSVEEYGDVDGSGSVNVADLTYFVAYM
                     FRGGPPPPVLNNADVNGDCELTVADLTALIGYLFRGGPAPVAGCVE"
     gene            1917..2732
                     /locus_tag="PK112_02460"
     CDS             1917..2732
                     /locus_tag="PK112_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10943.1"
                     /translation="MEFHALQHLRIIDQDRIAPFEWDDGGRIDLSPEGPGIFRDPLLV
                     TPLEGDDFLLLDDSRTYYSLVDADFEHLPVQICTPVELMVDSDPLSLTRFTHDDLTRV
                     LARFPDRIEILPAEKTVPDSPDHLDLIFGFVGAAPIRVRIRHSSLTGCPVPFDAVFRA
                     ILESGAFYPVVRDGGEGEWGDHSGPHSGTVSLPTFTLEDLRRAVSSGRLFPPGLIRVQ
                     AVCRVLNIDFPVPVLADRSTLEQKQAFLAELIALRARRRRIACFDGRVYMLNR"
     gene            complement(2917..3177)
                     /gene="rpsT"
                     /locus_tag="PK112_02465"
     CDS             complement(2917..3177)
                     /gene="rpsT"
                     /locus_tag="PK112_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HPC10944.1"
                     /translation="MPHHKSNFKRMRQSEKQRDRNRAWRSHLRGAIRDLRATEPGAPD
                     LAGKYRTVVSMIDRAASRRLLHRRTADRYKSRLARHAASKPA"
     gene            3293..>3848
                     /locus_tag="PK112_02470"
     CDS             3293..>3848
                     /locus_tag="PK112_02470"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HPC10945.1"
                     /translation="MSAWTAFSRVLGLVREQVMAFIFGAGMATDAYVTAFRIPNLLRD
                     MFAEGALSSAFVPTFKERLVAGSKDEAFHLANVVMTAILVVVGLVVLLGIVAAPLLIY
                     VFAHGFTADAVKFDLTVSMTRIMMAYLLLVSLSALVMGMLNSFGRFGLPALSPAMFNL
                     GTIVVVLAFYKVMDPPIYTMAIGVL"
BASE COUNT          591 a         1362 c         1122 g          773 t
ORIGIN      
        1 ccaccattga acgcctcacc gacctggtca cgatgcccga gaactcgccc ggcgggtccg
       61 atcaccagtc gttcatcgat cagggattcc ccgtctccaa cagcatcgag gggacgttca
      121 actaccccgg ctggcacacc aacctcgacc tgactagcga gatgaatttc ccgtttttca
      181 cgaaagtagt gaaagccggt ctggccgctc tcgccatcgt cgccaactcc gcccggccga
      241 ctgaaatcga gcgtctggtg gatatgggcg acggccagtc gatcgaggtt atctggaaga
      301 gctgcgatcc ctcctaccgc tacgtcgtgc gctgggggac agcctcgggc gtttataccg
      361 attccgccgt cgtcccgccg ggcggctgct cctatgtcgt caccggcctc accgagggcc
      421 agccgtattt cttcacggcc gaaggcagcg tcgaggactc ctatccgtcg atctactcca
      481 cggaggacag tctgacgccg ctggtggtgc cgcgccggcc gagcgccctg gtggccgacc
      541 cggcgctcca ggccatcgcc ctctcctggc gtcccaacac cgagggagac ctcaccggct
      601 ataagatcta tcggaaaatc gaggactacg actatcagtt gctggccggg ggcgtcaccg
      661 gtcagtctta taccgacagc gccatcgcgc cgcactccct cttcacttac cggatctccg
      721 ccgtcgacgg tgatgcccat gagtcggagc tttcgcccga atcgggcaac tatgccgcca
      781 cgttcgacgg cggcattctg atcgtggacg aaacctccca gcacccctcg gtcccgaccc
      841 aggcgggcca ggtggccttc tgggacacga tcctggggcc cacgccgtat tcccttgagg
      901 tgaccgattt cgccggcgcc gcgcttcggc gcgggatcgc cgggcagtac tcctcgatct
      961 tctggttcga cgacgatttc accaaccact tcctgaagac ctcacaggac tcgctcacct
     1021 ggtatgccgg ctacaccggc aatattctgg tcgccggtct gcgcaccgtg cagtactgga
     1081 ccagctccgt catctacccc gacgacttcg tctacaccga cttcggcctc gcctcctgga
     1141 cggagaactc ggccttcgat ttcgccggcg cccacgggca gaacggctgg cccagcctga
     1201 cggtccgccc gaacgtcttc aacaacctgc cctatgtgcc gagtctccag ctcgctcccg
     1261 gcgccaccct cctgtacacg tacaattcgg ccaccgacaa tccggcccgc gagggccaac
     1321 cggtcggcat tctgaaaacc acgccccacg gcgcgagaat cgtgctggcg ttcccggtca
     1381 tgttcctcac cgactcctcg gccacgcagc tgatgaactt cgtcaaagcc cagttcggcg
     1441 aatccggcag cgtggaggag tatggggacg tcgatggttc cgggtcggtg aatgtcgccg
     1501 atctgaccta cttcgtcgcg tacatgtttc ggggcgggcc gccgccgccg gtgctgaaca
     1561 acgccgatgt caacggcgac tgcgaactca cggttgccga tctcaccgcg ctcatcggct
     1621 atctgttccg cgggggaccg gccccggtgg ccggttgcgt ggagtaggct gcaacgacaa
     1681 gaggtctcgg gcgaaaacat ggaccggccg ggagacagcc ccggccggtc ttcttttggg
     1741 cgcaagaccg ccggaatccg ccctatgacc ggctgtgcac aatctgtgtc ggccgcagcc
     1801 ccggccccga gttttggcgc ccccgccgcg gcgccgggac acaattcaac ctgttggtaa
     1861 ttagacgctt gtctcagccc ggcaacgacg gcacagggct tgctccactt tcgcccatgg
     1921 agtttcacgc actgcagcac ctgcgtatca tcgatcagga ccgtatcgcc ccgtttgaat
     1981 gggatgatgg cgggcgtatt gacctgtccc ctgaggggcc ggggatcttt cgcgaccctc
     2041 tgctggtcac cccgctggag ggcgacgact ttcttctttt ggatgattcc cggacctact
     2101 actcgctggt cgatgccgac ttcgagcacc tcccggtcca gatctgcacc ccggtcgagt
     2161 tgatggtgga cagcgatccg ctcagcctga cccggttcac ccatgatgat ctcacccgcg
     2221 tcctggcccg tttcccggac cggattgaga tcctccccgc cgagaaaacc gttcccgact
     2281 cccccgacca tctcgatctc atattcggtt ttgtcggcgc cgcgccgatc cgcgttcgga
     2341 tccgccactc cagcctcacc ggctgtccgg tgccgttcga tgcggtcttt cgggccatcc
     2401 tcgaaagcgg ggctttctac ccggtcgtcc gggacggggg agagggggag tggggcgacc
     2461 actccggacc gcactcgggg acggtgagcc tgccgacctt caccctcgag gaccttcgcc
     2521 gggccgtgtc cagcggacgg cttttcccgc ccggtctcat ccgggttcag gcggtgtgcc
     2581 gcgtgctcaa catcgacttc cccgttcccg tgctcgccga tcggtctacg cttgagcaga
     2641 agcaggcatt tctggccgag ctcatcgctt tgcgggcgcg gcgccgccgg atcgcgtgtt
     2701 ttgacggccg ggtgtacatg ctcaaccgct gagttcccct ccggcgcccg tccgggcggc
     2761 cgcgttctgc tcccctctga aaatacttgt tgaagcccct gcgcgcgccc cgctactttt
     2821 catcccatgg agaacttcgc gccggccgcc gccccaacgc cgaacgcccg gctgcgttgc
     2881 cgcgccgggc gctgtgctcg atccggtgtc gccggatcag gccggtttgc tcgccgcgtg
     2941 ccgcgccagc cgggatttgt accggtcggc cgtgcgccgg tggagcaggc ggcgggaggc
     3001 cgcccggtcg atcatcgaga ccacggtgcg gtatttgccg gcgagatccg gggccccggg
     3061 ttccgtcgcc cgcaggtcgc ggatggcgcc gcgcaggtgg gaccgccagg cccggttccg
     3121 gtcccgctgc ttctccgact gcctcatccg cttgaagttc gatttgtgat gcggcatact
     3181 atcctttcac atcttccttt gctttcagaa ataaccttta aatatacgcg gcacgggcag
     3241 cttgtcaact cctgaggcaa agaaaagttc cctcctggga tcagccacaa cggtgtccgc
     3301 ctggactgcc ttctcccgcg tcctcggcct cgttcgcgaa caggtcatgg ccttcatctt
     3361 cggcgccggc atggccaccg atgcctacgt caccgccttc cgcatcccca acctcctgcg
     3421 cgacatgttc gccgaagggg ccctgtcatc cgccttcgtc ccgaccttca aggagcggct
     3481 ggtcgccggc agcaaggacg aggcttttca cctggccaac gtggtcatga ccgccatcct
     3541 cgtcgtcgtc ggcctcgtgg tgctcctcgg catcgtcgcc gcgccgcttc tcatctacgt
     3601 tttcgcgcac ggtttcaccg ccgatgccgt caagttcgac ctcacggtga gcatgacccg
     3661 gatcatgatg gcgtacctgc tgctggtgtc gctctcggcc ctcgtcatgg gcatgctcaa
     3721 ttctttcggc cgcttcgggc tcccggcgct ctcgccggcc atgttcaacc tcggcaccat
     3781 cgtcgtggtc ctggctttct acaaggtgat ggacccgccg atttacacca tggcaatcgg
     3841 cgtgctcg
//
LOCUS       DAOOKS010000101         3812 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_29030_length_3812_cov_3.116849, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000101 DAOOKS010000000
VERSION     DAOOKS010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3812
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29030_length_3812_cov_3.116849"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="PK112_02475"
     CDS             complement(<1..714)
                     /locus_tag="PK112_02475"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPC10946.1"
                     /translation="MTYPLLTLKAGRENAPANRHPWIFSGALAEGKPEVPHGALVRVA
                     EASGQVIATGTYSAKGMIAVRVLAWEEAPITREWIRERVREAVERRRLVGIGADETVT
                     GYRAVFGEADGLPGLVVDRYGEVAVVQFATAGAEGLREPILQALADVLTPAAIYERSD
                     LPARTEEGLAPASGLLSGVLPERVEFAEHGLRFTADVTGGQKTGFFLDQRDVRRQARA
                     LAAGRRVADLFSYRGAAGVA"
     gene            916..2325
                     /locus_tag="PK112_02480"
     CDS             916..2325
                     /locus_tag="PK112_02480"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HPC10947.1"
                     /translation="MDFPLFYLDFIGNRLLMAAVAITHVMINHPMAVGAWPLVALLEW
                     WGRRRGDLDLDALAYKITFVLFIITTTFGALTGVGIWLTAALIAPFGIGSLIRVFFWA
                     WFIEWLVFISEVGLVIVYFLMWQRWREGRWKALHNAVGAVLAVGSWLTMAIIVAILGF
                     MMGTGSWAADRSFFSAVFNPLYLPQLVFRTGLAMVTAGFTVWFLTFFFTRTRPELRRR
                     VVRIVSGWVLAWTPVVLIGAVWYWRRVPAPMAANLDVAFLTQRLTQWYDTFLYVVAAV
                     LALTVLIALAGLARPALIPRAVLVIPFLLGLYTLGHFERVREFIRKPHVVADYMYSNG
                     VTMSELPIFQRDGILTYSAYSTVRQVTSANKVDAGRDVYMLACSRCHTVSGVNSVLTR
                     FRNLYGDTPWDPAVLSSFIAVMHNTRTFMPPFPGAPAEADALAAYLRDLQERPRGLPG
                     AQAVGYSPRAAAAAAGPGQ"
     gene            2341..3729
                     /locus_tag="PK112_02485"
     CDS             2341..3729
                     /locus_tag="PK112_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HPC10948.1"
                     /translation="MAPLDLAARVPRDLSLPLPLDPAVIEVFLVVLFLLHIVFVNLMV
                     GGSLLTVVCEIVGRSHPRYDALARRIGATITVSKSLAVVLGVGPLLCINLLYTLYFYS
                     ANALTGYAWISLVPLVITAFLLSYLHKYTWERWTGPAKNHHIVIGAAGAFFFLMIPLI
                     FLANINLMLFPDQWSAVRGFFSSLQYGNVFPRYFHFLTASLAVTGLFLAGWFGRRRFP
                     VEQLLPAFTRAELRGLFYRLAFWATAAQLVFGPLLLLTLPLRGISWTLLAVVGVAAVF
                     VVLILALLWRETRAGNPAIGRRYGAVIALFTVLALFMATGRHLYRETALSRHRLAVAD
                     RTATFAAVELAARMRLAAGLGVGETVGGAPTGPSVFSNCAACHALDKVLAAPSIKEIY
                     SLYANNPAGIVQWAKNPGKKRPQFQPMPSMAHLGDEKLQLVADYMLSLGGGDGDANAA
                     DQPAGSPEQSRP"
BASE COUNT          501 a         1397 c         1162 g          752 t
ORIGIN      
        1 cgcgacgccc gccgctcccc ggtaggaaaa caggtcggcc actctccgcc cggccgccag
       61 cgcccgcgcc tgccgccgca catctctctg atcgaggaaa aaccccgtct tctgcccgcc
      121 cgtcacgtcg gccgtaaacc tgagcccgtg ttccgcaaac tccactcttt ccggaagcac
      181 tccggagagc agcccgctgg ccggcgcgag gccttcctcg gtgcgcgcgg ggagatcgct
      241 ccgctcgtag atcgccgccg gcgtcagcac gtcggccagc gcctgcagga tcggctcccg
      301 cagtccctcc gcgccggccg tcgcgaactg cacgaccgcc acctccccgt accgatcgac
      361 gaccagcccc ggcagcccgt cggcctcccc gaacaccgcc cggtatccgg tgactgtctc
      421 gtctgcgccg attccgacca gccttcgccg ctcgaccgcc tcccgcaccc gctcgcggat
      481 ccactcccgc gtgatgggcg cttcctccca cgccagcacg cgcaccgcga tcatcccctt
      541 ggccgagtac gttcccgtcg caatgacctg gcccgaggcc tcggctaccc ggacgagcgc
      601 gccgtggggg acctccggtt tgccctcggc gagggcgccg gagaagatcc atggatggcg
      661 gttggccggg gcgttttcgc gcccggcttt gagcgtgagc aacgggtacg tcatagcggt
      721 ggaatatacg caaatccccg gccggtgcaa ccgcgcatcg ttcgccggcg ggtcggttcc
      781 gattaataat tgacggcaca gcccgcgttg cgtacctttg agtcctcgtg cggccggcgc
      841 ttaaggccgg ggcggcggcc ggtatcgtgg gaaccggcgg gcgggcgttc ccgccgccgc
      901 agcgtgcgag gagccatgga ttttccgctg ttctatctgg atttcatcgg caatcgcctg
      961 ctcatggcgg cggtggcgat cacccacgtc atgatcaacc atcccatggc ggtcggggcc
     1021 tggccgctgg tcgcgctttt ggagtggtgg ggacgccgcc gcggcgacct cgacctcgat
     1081 gctctcgcct acaaaatcac cttcgtcctg ttcatcatca ccacgacctt cggcgctctc
     1141 accggggtcg ggatctggct caccgccgct ctcatcgccc ccttcggcat cggcagcctc
     1201 atccgcgtct tcttctgggc ctggttcatc gagtggctgg tgttcatcag cgaggtcggc
     1261 ctggtcatcg tgtacttcct gatgtggcag cgctggcgcg aaggccgttg gaaagcgctg
     1321 cacaacgccg tgggcgccgt cctcgccgtc ggctcctggc tcaccatggc catcatcgtc
     1381 gccatcctcg gtttcatgat ggggaccggg tcctgggcgg ccgaccgcag cttcttctcg
     1441 gccgtcttca acccgctcta cctcccccag ctggtgttcc gcaccgggct ggccatggtc
     1501 accgcgggct tcacggtctg gttcctgacc ttcttcttca cccggacccg ccccgaactt
     1561 cgccggcggg tagtgcgcat cgtgtccggg tgggtgctcg cctggactcc tgtcgttctc
     1621 atcggggctg tctggtactg gcggcgcgtg cccgcgccga tggcggccaa cctcgatgtg
     1681 gctttcctta cccagcgcct gacccagtgg tacgatactt tcctctatgt cgtcgcggcc
     1741 gtgctcgccc tcaccgtcct gatcgcgctg gccggcctgg cccgcccggc cctgattccc
     1801 cgggccgtgc tcgtcatccc cttcctgctc ggtctctaca cgctcggcca cttcgagcgc
     1861 gtgcgcgagt tcattcgcaa accccacgtt gtcgccgact acatgtactc caacggggtg
     1921 accatgtcgg agctgccgat tttccagcgc gacgggattt tgacgtactc cgcctacagc
     1981 accgttcgcc aggtcacttc ggccaacaag gttgacgccg gccgcgatgt gtacatgctc
     2041 gcctgctccc gctgccacac ggtgagcggg gtcaacagcg tcctgacccg tttccgcaac
     2101 ctctacggag acacgccctg ggatccggcc gtcctgagtt ccttcatcgc cgtcatgcac
     2161 aacacccgca ccttcatgcc gccgtttccc ggcgccccgg ccgaggcgga tgccctggcg
     2221 gcctatctga gagacctgca ggaacggccg cgcggtctgc cgggcgcgca ggcggtcggg
     2281 tattccccgc gggccgcggc cgccgcggcc gggccggggc agtgagggga ggtgatgacg
     2341 atggctccac tagaccttgc cgcccgcgtc ccgcgcgatc tgtcgttgcc tctgccgctc
     2401 gaccccgccg tgattgaagt gttcctggtc gtcctgtttc tcctgcacat cgtgttcgtc
     2461 aatctgatgg tcggcgggtc gctgctgacg gtcgtgtgcg aaattgtcgg ccgctcccat
     2521 ccccgttacg atgccctcgc gcggcggatc ggcgccacca tcaccgtcag caagagcctc
     2581 gccgtggtgc tcggcgtcgg tccgctcctg tgcatcaacc tgctctatac gctctacttc
     2641 tactcggcca acgcgctcac cggctatgcc tggatttcgc tcgtcccgtt ggtcatcact
     2701 gcgttcctcc tgagctacct ccacaagtac acctgggagc gctggaccgg gccggcgaag
     2761 aaccatcaca tcgtgatcgg ggccgccggg gcgttcttct tcctgatgat cccgctcatc
     2821 ttcctggcca acatcaacct gatgctcttt cccgaccaat ggtccgcggt gcgcgggttc
     2881 ttttcgtcgc tgcagtacgg caacgtcttc ccgcgctact tccatttcct gaccgcctcg
     2941 ctcgccgtca ccggcctgtt tctcgcgggc tggttcggcc gccggcgatt cccggtcgaa
     3001 cagctgcttc ccgcttttac ccgggccgag ttgcgcggtc tcttctaccg cctcgctttc
     3061 tgggcgacgg cggcgcaact cgtctttggg cctctgctgc tgctcacgct cccgctgcgt
     3121 ggcatcagtt ggactctgct cgccgtcgtc ggcgtcgctg cggttttcgt cgttctcatt
     3181 ctggccctgc tctggcggga gacccgcgcg ggcaatccgg cgatcgggcg ccgctacggc
     3241 gcggtcatcg ccctgttcac cgtccttgcg ctcttcatgg cgaccggccg ccatctctac
     3301 cgcgagaccg ccctcagccg gcaccggctc gcggtcgccg accgcaccgc tactttcgcc
     3361 gcggtggagt tggcggcccg gatgcgtctg gcggccgggc tgggggtcgg cgagacggtc
     3421 ggcggcgctc cgaccgggcc gagcgtcttc tcaaactgcg ccgcctgtca tgcgctcgac
     3481 aaggtcctcg ccgcgccgtc catcaaagag atctactcgc tctacgccaa caacccggcg
     3541 ggcatcgtcc agtgggcgaa gaaccccggt aagaaacgcc cgcaatttca gcctatgcct
     3601 tcgatggccc acctcggcga cgaaaaactg cagctggtgg cggactacat gctcagcctg
     3661 ggcgggggag atggggacgc aaatgcagcg gaccagcccg ccgggagccc cgagcagagc
     3721 cgtccgtagc gcagggcgga tagttcccac gaaggatgtt gtgtcggtca ggaccacaag
     3781 ggtcgtgacg ggaccgctta cgtcgttggc ag
//
LOCUS       DAOOKS010000102         3798 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_29160_length_3798_cov_3.570131, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000102 DAOOKS010000000
VERSION     DAOOKS010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29160_length_3798_cov_3.570131"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..646)
                     /locus_tag="PK112_02490"
     CDS             complement(113..646)
                     /locus_tag="PK112_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10949.1"
                     /translation="MMTARLIFLCFGPALMLLAPAGAAAPTGPSGTTALGWGPHVGLT
                     VDPDQIHLGVHLQVGPPQPGVRFRPAFDLGFGDNVTAIALNFDAVCLLRTRDPRLMPY
                     LGAGIGANYYDFDRGVSARRRDDRDEGDGSDVEPGVNLLGGLDWRLRGGAHFFGEARI
                     GLVDAPDLKLTIGWTFF"
     gene            942..2315
                     /locus_tag="PK112_02495"
     CDS             942..2315
                     /locus_tag="PK112_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPC10950.1"
                     /translation="MPNILVVDDKDSMRNMLAETLSEEGHRVDAALDGRSAIDLVRNK
                     SYDLVLTDLKMPDISGLEVLTTVKEVDSETAVIVMTAYGTIEDAVAAMKAGAYDFITK
                     PFDTEHLCVLINRALENRRLMAENTLLREEAMSGKEFANIIGKNHQMAEVCALVQKVA
                     GSDASVLLQGESGTGKELFARAIHGLSSRANGPYITINCAAIPRELLENELFGSEKGA
                     FTGAHARKMGKFEIANTGTIFLDEIGDMDIALQAKLLRVLQQRNFERLGGTKPIEVDV
                     RVIAATNMDLTELIRAKRFREDLYYRLSVFPLTIPPLRERLDDLPLLADFFIAKYCTE
                     MRKPVKSLTRPALELLQKYHWPGNVRELENTIERAVILAEGKKITPDHLAIRLRRTSE
                     IQLRDGAGLKEIGALAQMQAERSVILRVLKDTRGNKRKAARILKIDYTTLFDKIKKYD
                     IESALNE"
     gene            2862..>3798
                     /locus_tag="PK112_02500"
     CDS             2862..>3798
                     /locus_tag="PK112_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10951.1"
                     /translation="MFTTGRIRRLAVLAPVAALALIFACSDSGTDGPPVEGDCTTLTA
                     QANAALAALMQGDIDSIINHGDSTFRPHDVDFSIPHGLYQQALAADPNCADAKMGAAF
                     TGVLMFMTDADFNSLVEKFKHAYDTLTFNPMRPNSLAPAVGFGGDILTDAIPLKAGRF
                     TGLIPSPLELDRAALRLAAVDPDLTALQDRLEESLLPKIRSARTRLLDLVADPDYTFE
                     ITPAMQGNEGAEPVLLDRSDIQLFIAMTYAAEAGLEVFFARNLSLSAYTTAGVEEALA
                     RTSEFLSLKSGGVGTQHMGNAKQCVLDAVNALKTAI"
BASE COUNT          720 a         1327 c         1137 g          614 t
ORIGIN      
        1 gccagcgccg ccagcaacag gatcgccagg ctgccgcaca aagcgttctt catcgtgatt
       61 ctcctccgtg aaggattgct ggggactagt atcggcattt cgccggctgc cgttagaaga
      121 atgtccaccc gatcgtcagc ttcaaatccg gggcgtcgac caggccgatc ctggcctccc
      181 cgaaaaagtg tgcgccgccg cgcagccgcc agtcgagccc gcccagcagg ttgactcccg
      241 gctcgacgtc ggagccgtcg ccctcgtcgc ggtcatcgcg ccggcgcgcc gaaacgcccc
      301 gatcgaaatc gtaatagttg gccccgattc ccgccccgag atacggcatc aggcgcggat
      361 cgcgggtccg aaggagacac accgcgtcga aattgagcgc tatcgcggtg acgttgtccc
      421 cgaacccgag gtcgaatgcg gggcggaacc gcactcccgg ttgcggcggg ccgacctgca
      481 agtgcacacc caggtgaatc tgatccgggt caaccgtcag gccgacatgg ggtccccagc
      541 cgagcgcggt cgttcccgat ggccccgtcg gagctgccgc cccggcgggc gcaaggagca
      601 tcagcgccgg gccaaaacac aggaagataa ggcgggccgt catcatggag gatctccttt
      661 ccgtcaatct cgttcgcgat tgccgggttc tcgtcagtcc gcttgcaggg agagaagcgc
      721 ggcgtggtac tgccgatcaa gatacggcgc aagcgcgccg ggcgcaaggg gggcgtcgcg
      781 gttcgggggc ggtgcccggc ggctcttcgg accttccctg cccaaccctt gcacaccccg
      841 ccgcccgcgc ccggcaaatt cctcctgccc cctccaaaac tataggattc ttccgatata
      901 tccctcgata gcagattccg aaccagacag ggagccagcc gatgcccaat attctcgtgg
      961 tcgacgacaa ggactcgatg cgcaacatgc tcgcggaaac gctcagcgaa gagggccacc
     1021 gggtcgacgc ggccctggac ggcaggagcg cgatcgacct ggtccgcaac aagtcctacg
     1081 atctcgtcct caccgacctg aaaatgccgg acatcagcgg cctcgaggtg ctcaccacgg
     1141 tgaaggaagt ggacagcgaa accgccgtca tcgtcatgac cgcctacggg acgatcgagg
     1201 acgcggtggc ggcgatgaaa gccggcgcct acgacttcat caccaaaccc ttcgacaccg
     1261 agcacctgtg cgtgctgatc aaccgcgctc tggagaaccg ccgcctcatg gccgaaaaca
     1321 cgctgctgcg ggaggaggcg atgagcggca aggaattcgc caacatcatc ggaaagaacc
     1381 accagatggc cgaggtctgc gccctggtgc agaaggtcgc cgggtcggac gcctcggtgc
     1441 tgctccaggg ggagtcgggc acgggcaagg agcttttcgc ccgcgccatt cacggcctct
     1501 cgtcgcgcgc caacggcccc tacatcacca tcaactgcgc cgccattccg cgcgaactgc
     1561 tcgagaatga gctgttcggt tccgagaagg gggccttcac cggcgcccac gcccggaaaa
     1621 tggggaaatt cgagattgcc aataccggga cgatcttcct cgacgaaatc ggcgacatgg
     1681 acatcgccct ccaggcgaaa ctcctgcgcg tcctccagca gcgcaatttc gaacgcctcg
     1741 gcggaaccaa accgatcgag gtcgacgttc gcgtgatcgc cgccaccaac atggatctca
     1801 ccgaactcat ccgcgcaaag cggttccgcg aggatctcta ctaccggctc tcggtttttc
     1861 cgctcaccat cccgccgctc cgcgagcgtc tcgacgatct gccgctgctg gccgatttct
     1921 tcatcgcgaa atactgcacc gagatgcgca agccggtgaa atcgctcacc cgtccggccc
     1981 tggagctgct ccagaaatac cactggccgg ggaacgtccg cgaactggaa aacacgatcg
     2041 aacgcgcggt cattctcgcc gagggcaaga aaatcacgcc cgaccacctg gctatccgcc
     2101 tccgccgcac gagcgaaatc cagttgcgcg acggcgcggg gctcaaggag atcggcgccc
     2161 tggcccagat gcaggccgag cgcagcgtca tcctccgcgt ccttaaagac acgcgcggca
     2221 ataaacgcaa ggccgcccgc atcctcaaga tcgactacac cactctgttc gacaagatca
     2281 agaagtacga catcgagagc gccctgaacg aataggcgcg tccgccggtc gctccagtaa
     2341 aatgcgaaag gccccgccct ccggcggggc ctttgtgctg actgcgccaa ttgcagaccg
     2401 taggtatgcg ccgaccggcc gccgctggcc ggcggcggag tcgtcgcgcc gaagcagacg
     2461 ccgtgcaccg ccggtgcgaa aggggagagg tcgcggacgc cgaacgtcgt gaaccttcgg
     2521 tgtccccaga ttgccgttcg gagtccctgt gcaaacacag tcgagcaggt ggtcgtcacg
     2581 gacggagcca tcgtacatac gcgcggtcga atgctcccaa gggaagctgc tccccggcag
     2641 cctcgccaac agccggcgcg tcatcctgcg gcggcgctgc gttcaacatc ctgcgtcctt
     2701 gccgttccga tcccggttgt ccgcgttggc ggccggtgcg aagattccgc ttggcgaacc
     2761 cgcctgccgg cggtaaattg acagatgttt cagggggtcg cgcagggtga cttggtgtct
     2821 gctcgacaac ctgccgcaca tccacagact caggaggtct catgttcacc accggccgca
     2881 tccgtcgctt ggcggtgctt gcccccgtgg cggcactggc gctgattttc gcgtgcagcg
     2941 attcggggac cgacggtccc cccgtcgagg gcgactgcac gactctcacc gcccaggcaa
     3001 acgccgccct ggccgccctc atgcagggcg acatcgacag catcatcaat cacggcgact
     3061 cgacttttcg cccgcacgac gtcgacttca gcattcccca cggcctctac cagcaggccc
     3121 tggcggccga tcccaactgc gcggacgcca agatgggcgc ggcattcacg ggcgtcctca
     3181 tgttcatgac cgacgccgac ttcaacagcc tggtggagaa attcaagcac gcgtacgaca
     3241 cgctgacgtt caatccgatg cgcccgaaca gcctcgcccc cgcggtcggt ttcggcggcg
     3301 acattctcac cgatgctatt cccctcaaag cggggcgctt caccggcctg atcccctcgc
     3361 cgctcgagct cgatcgcgcg gcgctgcggc tggcggcggt cgatcccgac ctcaccgctc
     3421 tgcaggatcg gctcgaagag tcgctcctgc cgaaaatccg gagcgcgcgc acccgcctgc
     3481 tcgacctggt cgccgatccc gactacacgt tcgagatcac gccggccatg cagggcaacg
     3541 aaggcgccga gcccgtcctt ctcgaccggt ccgacatcca gctctttatc gccatgacct
     3601 acgctgcgga ggcggggctg gaggtctttt tcgcccgcaa cctttccctt tcggcctata
     3661 cgacggccgg cgttgaagaa gccctggccc gcacttcgga gttcctcagc ctcaagtccg
     3721 gcggcgtcgg cacgcagcac atgggcaacg ccaagcagtg cgtcctcgat gccgtcaacg
     3781 ccctcaagac cgcgatcg
//
LOCUS       DAOOKS010000103         3777 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_29389_length_3777_cov_2.718700, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000103 DAOOKS010000000
VERSION     DAOOKS010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29389_length_3777_cov_2.718700"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="PK112_02505"
     CDS             <1..607
                     /locus_tag="PK112_02505"
                     /inference="COORDINATES: protein motif:HMM:NF014874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HPC10952.1"
                     /translation="DAGMMTVVLEGRTFRMPLVGKHQVYNLTCAYALFRSLGYRFDGV
                     ETAQIELASAPMRGQLVARGGVRVLVDCYNANPENVRAGLAGFFALPTAGRRVVVLGD
                     MLELGPESPRYHRESGAVLARYPFDLAVLVGPRSRETLEGAVASGADRGRLRHCADAA
                     GCAKEIAAIVQKNDLVYLKASRGIGLEAVLNGLGEPGEEVL"
     gene            607..1695
                     /gene="mraY"
                     /locus_tag="PK112_02510"
     CDS             607..1695
                     /gene="mraY"
                     /locus_tag="PK112_02510"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HPC10953.1"
                     /translation="MLYHLLYPLAHQVSGLNLFRYITFRTAGAMATAIFICLILGPFF
                     IGLLRKHQVKERIRAEGPASHQKKAGTPTMGGLIIVSGIIIPTLLWSDLTNFYVLMAL
                     LVTAWLGLLGYLDDYSKALKHQRNGMVARKKFMGQVGLGLVFGGLLLWVAPNGLYDGM
                     TGIPFFKNYMLDLGVLYIPWMILVLTGASNAVNLTDGADGLAIGLSGLAFLAFGGIAY
                     LTGRLDFSSYLQIEYLPGAGELTVFCGAAIGSALGFLWFNSHPAEVFMGDTGSLALGG
                     ALGAIAIMLKKELLLVIVGGVFVVEVLSVIIQVLSYRYRGGKRVFKMAPIHHHFELLG
                     WPEEKVVVRFWIIGALCTLATLATLKVR"
     gene            1700..3091
                     /gene="murD"
                     /locus_tag="PK112_02515"
     CDS             1700..3091
                     /gene="murD"
                     /locus_tag="PK112_02515"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HPC10954.1"
                     /translation="MTREERIRGRKVGVIGMARSGLAAALLAKEMGGIPFVTDSGRRE
                     ALAPACAQLEKSGVPFETDGHSDRILQSDYAVISPGIPLSAEIARRIRERGIPLFSEI
                     EFASWFCRGRIVAITGSNGKTTTTTLAGEMFAAAGRPTCICGNIGRPFAEAASSVPPD
                     GVAVVEVSNFQLETIADFRPAVAAILNITADHLDRHGTMEEYKRTKYRITENQGPDDV
                     LVTNLDDPVTSGERIATRAARVYFTTGDATEAGAFVRAGVLWRRKDRAEEPVIPARDI
                     LIPGPHNLQNAAAATVMATVFGVEAAVLAGVLRTFPGVEHRLERVPSVAGVHFINDSK
                     ATNVDSVCFALQSVDTPVYLILGGRDKGAPYEPLAPLARGKVRGVVAIGEAREKIFRA
                     LGREFPVQFADTLEEAVQKCFAQARPGETVLLSPACASFDMFDNYEHRGRAFKEAVAQ
                     LKNGKKNHNPVAP"
     gene            3081..>3777
                     /locus_tag="PK112_02520"
     CDS             3081..>3777
                     /locus_tag="PK112_02520"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HPC10955.1"
                     /translation="MLLKGGYDEKLLIAYLVAVVAGMVLVYSASSMLAESRFGSHLHF
                     IGRQFVWAIISLAAIWLVCRIDLQRWAVWSWLLFVLTLGLLALVFLMPARNGSHRWLF
                     LGPLTLQPAELFKFVMIYFLAFSLANRNRDVTKLKQVWMPYGPMLGAGLVLIVLEPDL
                     GMSMVIFATAVGLFFLAGAKIKHIAYAALPAMGGAALLVFVIGYKKARVMDFLASVMD
                     PLAGSYQSKQAALT"
BASE COUNT          569 a         1182 c         1356 g          670 t
ORIGIN      
        1 cgacgcgggg atgatgacgg tcgtgctcga ggggcggacg ttccggatgc cgctggtcgg
       61 gaagcaccag gtgtacaacc tgacctgcgc ctacgccctg ttccggtcgc taggctaccg
      121 gtttgacggc gtggagacgg cgcagataga attggcgtcg gcgccgatgc gggggcagtt
      181 ggtcgcgcgc gggggcgtgc gggtgctcgt ggattgctac aacgccaacc ccgaaaacgt
      241 cagggcgggc ctggcggggt tcttcgccct cccgaccgcc gggcggcgcg tggtcgtgct
      301 cggcgacatg ctggagctcg gccccgaatc gccccgctac catcgggaga gcggcgccgt
      361 gctggcgcgc tacccgttcg acctggcggt gctggtcggg ccgcgctcgc gcgagacgct
      421 cgagggagcg gtcgcgtccg gggccgaccg ggggcgcctg cggcactgtg ccgacgcggc
      481 ggggtgcgcg aaagagatcg ccgccatcgt gcagaaaaac gacctggtgt acctgaaggc
      541 ctcgcggggg atcggcctgg aagcggttct caacggcctg ggcgaaccgg gtgaggaggt
      601 tctgtaatgc tctaccacct gctctatcca ctggcgcacc aggtgtcggg gctcaacctg
      661 ttccggtata tcacgtttcg gacggccggg gcgatggcga cggcgatctt catctgcctc
      721 atcctcgggc cgttcttcat cgggctcctg cgcaagcacc aggtgaagga gaggatccgg
      781 gcggaagggc cggcgtcgca ccagaagaaa gccggaacgc cgaccatggg cgggctcatc
      841 attgtgtcgg ggatcattat ccccaccctg ctctggtcgg atctgacgaa tttctacgtg
      901 ctgatggcgc tgctggtgac ggcgtggctg ggactgctgg gctacctcga cgactacagc
      961 aaggccctca agcaccagcg caacggaatg gtggcgcgca agaagttcat ggggcaggtg
     1021 gggctcgggc tcgtgttcgg aggcctcctg ctgtgggtgg ccccgaacgg tctctacgac
     1081 gggatgaccg ggatcccgtt cttcaaaaac tacatgctcg acctcggcgt cctgtacatc
     1141 ccgtggatga tcctcgtgct caccggcgcc tcgaacgcgg tgaacctgac cgacggggcg
     1201 gacgggctgg cgatcgggct gtccggactg gccttcctcg ctttcggcgg gatcgcctat
     1261 ctcaccgggc ggctcgattt ctcgagctac ctccagatcg agtaccttcc gggcgccggc
     1321 gagctgaccg tgttctgcgg ggcggcgatc gggtcggccc tcgggttcct gtggttcaac
     1381 tcgcacccgg ccgaggtctt catgggggac accgggtcgc tcgctctcgg cggcgcgctc
     1441 ggcgcgatcg ccatcatgct caagaaagaa ctgctcctgg tgattgtcgg cggcgtgttc
     1501 gtcgtcgaag tgctgtcggt gatcatccag gtgctctcat accgctaccg gggaggcaag
     1561 cgggtgttca agatggcgcc gatccaccac catttcgagc tgctcggttg gccggaggag
     1621 aaggtggtgg tgcggttctg gattatcggg gcgctctgca cgctggccac gctggcgacc
     1681 ctgaaagtgc ggtaggacaa tgacgcggga agaaaggata cgcggacgca aggtcggggt
     1741 gatcgggatg gcccggtccg ggctggcggc ggcgctgctg gccaaagaaa tgggcggcat
     1801 ccccttcgtc accgacagcg gccggcgcga ggcgctcgcg cccgcctgcg cccagcttga
     1861 gaagtcgggc gtgccgttcg agaccgacgg ccacagcgac cggatcctgc agagcgacta
     1921 cgccgtgatc tcccccggca tcccgctctc cgcggagatc gcccggcgga tccgcgagcg
     1981 ggggattccg ctcttctcgg agatcgaatt cgcctcgtgg ttctgccgcg ggcgtatcgt
     2041 cgcgatcacc ggatccaacg gcaagacgac cacgacgacg ctggcggggg agatgttcgc
     2101 ggcggcgggc cgcccgacct gcatttgcgg caacatcggc cggcccttcg ccgaagcggc
     2161 ctcgagcgtg ccgcccgacg gggtggcggt cgtggaggtg tcgaacttcc agctcgagac
     2221 gatcgccgat ttccgcccgg ccgtggccgc gattctcaat atcaccgcgg accacctcga
     2281 ccggcacggg acgatggagg agtacaagcg gacgaagtac cggatcaccg agaaccaggg
     2341 tcccgacgac gtgctggtga cgaatctcga cgatccggtg acgtccggcg agcggatcgc
     2401 cacgcgggcg gcgcgggtgt atttcacgac gggggacgcg accgaggcgg gcgcgttcgt
     2461 gcgcgcgggg gtgctctggc gccgcaaaga ccgggccgag gagccggtca tcccggcccg
     2521 cgatatcctc atcccggggc cccacaacct gcagaacgcg gcggcggcga cggtgatggc
     2581 gaccgtgttc ggcgtcgagg ccgccgtact cgccggggtc ctgcgcacgt tcccgggcgt
     2641 cgagcaccgg ctcgagcggg tgccgagcgt cgcgggcgtg catttcatca acgactccaa
     2701 ggcgacgaac gtggattcgg tctgtttcgc gctgcagtcg gtggacacgc cggtctacct
     2761 gattctcggc gggcgcgaca agggcgcgcc ctatgagccg ctcgcgccgc tggcccgggg
     2821 aaaggtgcgc ggcgtggtgg cgatcgggga ggcccgggag aagatcttcc gggcgctggg
     2881 ccgggaattc ccggtgcagt tcgccgacac tctcgaggag gcggtgcaga agtgttttgc
     2941 gcaggcgcgc cccggggaga ccgtcctgct ctcgcccgcc tgcgccagtt tcgacatgtt
     3001 cgacaactat gaacaccgcg gccgggcctt caaggaggcg gtcgcacaat tgaaaaatgg
     3061 taagaaaaac cacaacccgg ttgctcctta agggcggcta cgatgagaag ctgctgatcg
     3121 cctacctcgt cgcggtggtg gcgggaatgg tgctcgtcta ctcggcctcc tcgatgctgg
     3181 cggagagccg gttcggctcg cacctgcact ttatcgggcg gcagttcgtc tgggcgatta
     3241 tttcgctcgc cgcgatctgg ctggtctgcc gcatcgatct ccagcggtgg gcggtgtggt
     3301 cgtggctgct gttcgtgctc accctcgggc tgctggcgct ggtctttctc atgccggcgc
     3361 ggaacggctc gcatcgctgg ctcttcctcg gacccctcac cctccagccc gccgagctgt
     3421 tcaagttcgt catgatctac ttcctcgcct tctccctggc caaccgcaac cgcgacgtga
     3481 cgaagctcaa gcaggtgtgg atgccctacg ggccgatgct cggggcgggg ctggtgctga
     3541 tcgtgctgga gccggatctc gggatgtcga tggtgatatt cgcgacggcg gtggggctgt
     3601 tcttcttggc gggggcgaag atcaagcaca tcgcctacgc ggctctgccg gcgatgggcg
     3661 gggcggccct gctggtgttc gtgatcgggt acaaaaaggc ccgcgtgatg gacttcctcg
     3721 cctcggtcat ggatccgctg gcggggagct accagtcgaa gcaggcggcg ctcacgc
//
LOCUS       DAOOKS010000104         3768 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_29498_length_3768_cov_2.982494, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000104 DAOOKS010000000
VERSION     DAOOKS010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3768
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29498_length_3768_cov_2.982494"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..175
                     /locus_tag="PK112_02525"
     CDS             <1..175
                     /locus_tag="PK112_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HPC10956.1"
                     /translation="SMDRVYLNIDRYGNTSAASVPLALDEANRAGMLHPGDHILLVAF
                     GGGLIWGSALVRW"
     gene            178..1113
                     /gene="fabD"
                     /locus_tag="PK112_02530"
     CDS             178..1113
                     /gene="fabD"
                     /locus_tag="PK112_02530"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HPC10957.1"
                     /translation="MSKTALFFPGQASQYVGMGRDLYEASADVRRLYELAADAMQFDI
                     ARLSFEGPAEDLRRTRFTQPAILLHSLAALTVLGDGRPAFDYAAGHSLGEYGALAVTG
                     ALTFEQAIRAVVRRAHLMEEACRQQPGTMAAVMGLSAAQVEQVCREAAAAGVVVPANY
                     NSGIQIAVSGSRPGVEKAIELAKSAGAKLAMLLEVGGAFHSPLMEPAREGLRTYLADL
                     AIAAPARAVIANVTAEPATDGATIRRLLIEQVTAPVKWAQTMACLAAEGVTTVYEIGP
                     GKVLTGLARRDMNLTTSVNLDTLADIEALGALQRP"
     gene            1129..1860
                     /gene="fabG"
                     /locus_tag="PK112_02535"
     CDS             1129..1860
                     /gene="fabG"
                     /locus_tag="PK112_02535"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HPC10958.1"
                     /translation="MDFSGKTVVVTGSARGIGRAIAERFARAGARVVISDVDDAALAA
                     AAAAMPGEAIGVRADVTSSADAEALIAAAQERFGSVDVVVNNAGVTRDTLLVRMDEKD
                     WDMVLDINLKGAFLVTKSAAKVMMKQRSGRIINVSSVVGLFGNAGQTNYAASKAGLLG
                     LTKSAAKELGARGITVNAVAPGFIETDMTRVLPEEARARFLERSVIKRAGTPDDVAAA
                     VMFLASDAAAYITGHVIAVDGGMAM"
     gene            1905..2153
                     /locus_tag="PK112_02540"
     CDS             1905..2153
                     /locus_tag="PK112_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HPC10959.1"
                     /translation="MAAELEDRVKEIIVEQLGVEASQVTEKAKFVDDLGADSLDTIEL
                     VMALEEEFSIEIPDEDAEKIVSVGDAITYIAEHSPKAK"
     gene            2195..3445
                     /gene="fabF"
                     /locus_tag="PK112_02545"
     CDS             2195..3445
                     /gene="fabF"
                     /locus_tag="PK112_02545"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HPC10960.1"
                     /translation="MKKRVVVTGMGVVSPNGNTLEAFWQSLLAGKSGAGRITRFDPEP
                     FPTQIAAEVKDFNPELYLEKKEARRMDLSEQYAVAASDMALVDAGLKDGRLDPDRIGV
                     VIGSGIGGISTFERQHSLMEETGGPAKVSPFFIPMMIIDMCAGLVSIRYGFRGPNYAT
                     VSACASSAHAIADAFRIIQRDEADVMVTGGAEATITRTALAGFCQAKALSVRNDEPER
                     ASRPFDAERDGFVMGEGAAILVLESLDHARARGVRIYAELLGAGMSGDAHHMTAPHPE
                     GLGARRAMAMALRDAGLAPEQIDYVNTHGTATILGDTAETRAIKEVLGPRAYEIPCNS
                     TKSMTGHLLGAAGALEVAACVLSLRDSVVHPTINIDTPDPECDLDYVREGKRAVTIRH
                     ALSNSFGFGGHNVSLVVGTVNGSA"
     gene            3445..>3768
                     /locus_tag="PK112_02550"
     CDS             3445..>3768
                     /locus_tag="PK112_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009772720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HPC10961.1"
                     /translation="MGVWDRLRRFFSPSQAQRRAAAPAAVQQLIGYHFRCPAVLELAL
                     THRSFAHAADQYHLSNERLEFLGDSVLGLVIAERLYQDNPDLREGELTKRKALLVSEA
                     TLAEIG"
BASE COUNT          634 a         1233 c         1315 g          586 t
ORIGIN      
        1 atccatggac cgggtgtacc tgaacataga ccgctatggc aacacctcgg cggcctcggt
       61 gccgctggcc ctggacgagg cgaaccgcgc ggggatgttg caccccggcg accacatcct
      121 ccttgtcgct ttcggcggcg gcctgatctg gggctccgct ctcgtgaggt ggtgagtatg
      181 agcaagacag cgttgttctt tccgggccag gcctcgcagt acgtcgggat gggccgggat
      241 ttgtatgagg cctcggccga cgtgcgccgc ctgtatgagc tggcggccga cgccatgcag
      301 ttcgatatcg cccgcctctc gttcgagggg ccggccgagg atctcaggcg cacccggttc
      361 acccagccgg cgatcctgct gcactcgctg gcggcgctga cggtgctggg ggacggccgg
      421 ccggcgttcg attacgccgc cgggcattcg ctcggcgagt acggggccct ggcggtcacc
      481 ggcgccctga cgttcgagca ggccatccgc gccgtcgtgc ggcgcgccca cctgatggag
      541 gaggcctgcc gccagcagcc gggaaccatg gccgccgtga tggggctgag cgccgcgcag
      601 gtcgagcagg tctgccgcga ggccgccgcc gcgggcgtgg tcgtgcccgc caattacaac
      661 tcgggaatcc agatcgccgt ctcggggtcc cggccgggag tggagaaggc gatcgagctg
      721 gccaaaagcg cgggcgccaa gctggcgatg ctgctcgagg tgggcggcgc tttccactcg
      781 ccgctcatgg agccggcccg cgagggactg cggacgtatc tcgccgatct ggcgatcgcc
      841 gctcccgccc gcgccgtgat cgccaacgtg accgccgagc cggcgactga cggtgcaacg
      901 attcgccgcc tgctcatcga gcaggtgacc gcgccggtga agtgggcgca gacgatggcc
      961 tgcctggcgg cggagggcgt gacgaccgtg tatgagatcg gtccgggcaa agtgctcacc
     1021 ggactggcaa gacgtgacat gaacctgacg acgtcggtca atctcgacac cctggccgac
     1081 atcgaggcgc tgggcgcgct gcagcgcccc tgagggaggc ggaagatgat ggattttagc
     1141 ggcaagacgg tcgtggtcac cggttccgcc cgcggcatcg ggcgggcgat cgccgagcgg
     1201 ttcgcccgcg ccggcgcccg cgtggtgatc agcgacgtcg atgacgccgc gctggcggcc
     1261 gcggccgccg ccatgccggg cgaggccatc ggcgtgcggg ccgacgtgac cagcagcgcc
     1321 gacgccgaag ccctgatcgc ggccgcacag gagcggttcg gttccgtgga cgtggtggtc
     1381 aacaacgccg gcgtgacccg cgacacgctc ctggtgcgga tggacgaaaa agactgggac
     1441 atggtgcttg acatcaatct gaaaggcgct tttcttgtca cgaaatcggc cgcgaaggtg
     1501 atgatgaagc agcgctccgg gcgcatcatt aacgtcagct ccgtggtcgg attgtttggc
     1561 aacgccggtc agaccaacta cgcggcgtcg aaagccggac tgctcggatt gacgaaatcc
     1621 gcggccaaag aactgggggc gcgcggcatt accgtcaacg ccgtcgcacc cgggttcatt
     1681 gaaacggaca tgacccgggt gctgccggaa gaagcgcgcg cccggttttt ggagcgcagc
     1741 gtgatcaagc gggccggcac gcccgacgat gtcgccgccg ccgtgatgtt cctggcgtcc
     1801 gacgcggccg cctacatcac cggccacgtg atcgccgtcg acggcggcat ggccatgtga
     1861 actggactga agacatacaa ccatactcag gaagaggtgc tactatggcc gcagaactgg
     1921 aagatcgggt gaaggaaatc atcgtcgagc aactcggcgt cgaagcttcc caggtgactg
     1981 agaaagccaa gttcgtcgac gacctcggcg cggattcgct cgacacgatc gagttggtga
     2041 tggcgttgga agaggaattc tcgattgaga taccggatga agatgccgag aagatcgtct
     2101 cggtaggcga cgccatcacc tatatcgcgg agcacagccc gaaggccaag taggcggcgg
     2161 gcaggccgtt cacggaagga acggaacctc aactatgaag aaacgtgttg tcgtgacggg
     2221 catgggggtc gtctccccga acgggaatac gctcgaggcg ttctggcagt cgctcctggc
     2281 cgggaaatcg ggggcggggc ggatcacgcg gttcgacccg gagccgttcc cgacccagat
     2341 cgccgccgag gtcaaggact tcaatccgga gctctacctg gagaagaaag aggcgcgccg
     2401 gatggacctc tcggagcagt acgccgtggc cgccagcgac atggcgctgg ttgacgccgg
     2461 gctgaaggac gggcggctcg accccgaccg gatcggcgtc gtcatcggct ccggcatcgg
     2521 cggcatcagc acgttcgagc ggcagcactc cctcatggag gagaccggcg ggccggccaa
     2581 ggtgtcgccg ttcttcatcc cgatgatgat catcgacatg tgcgccgggc tggtgtcgat
     2641 tcggtacggc ttccgcggac cgaactacgc caccgtctcc gcctgcgcct cgtcggccca
     2701 cgcgatcgcc gatgctttcc gcatcatcca gcgcgacgag gccgacgtga tggtcacggg
     2761 cggggccgaa gcgacgatca cgcggacggc gctggccggt ttctgccagg ccaaggcgct
     2821 gtcggtccgg aacgatgagc cggagcgcgc ctcgcggccg tttgacgcgg agcgcgacgg
     2881 ctttgtgatg ggcgaggggg cggccatcct cgtgctcgag tcgctcgatc acgcccgggc
     2941 gcgcggcgtc cgcatctacg ccgaactgct cggggcggga atgagcggcg acgcccacca
     3001 catgaccgcg ccgcaccccg agggcctcgg ggcgcgccgg gccatggcca tggccctccg
     3061 cgacgccggg ctggcgcccg agcagatcga ctacgtgaac acgcacggga cggcgacgat
     3121 cctgggggat acggcggaga cgcgcgcgat caaggaggtc ctcggcccgc gcgcctacga
     3181 gatcccgtgc aactcgacca agtccatgac cggccacctc ctcggggcgg ccggcgcgct
     3241 cgaagtggcg gcgtgcgtgc tatccctccg ggatagcgtc gtgcatccga cgatcaacat
     3301 cgacacgccg gatcccgagt gcgatcttga ttacgtccgg gagggcaagc gggcggtgac
     3361 gatccgccac gccctgtcca actccttcgg cttcggcgga cacaacgtgt cgctggtggt
     3421 cggaacagtc aacggctctg cgtgatgggt gtttgggacc gcctcagacg atttttctct
     3481 cccagccagg cgcagcggcg cgccgccgct ccggccgccg tgcagcagct catcggctac
     3541 cacttccgct gcccggctgt cctcgagctg gcgctcaccc atcggtcgtt cgcccacgcg
     3601 gccgaccagt accacctgtc caatgaacgg ctcgaattcc tcggcgattc cgtcctcggc
     3661 ctcgtcatcg ccgagcggct ctaccaggac aacccggatc tccgcgaggg ggaactgacc
     3721 aagcgcaagg cgctcctcgt cagcgaggcg accctcgccg aaatcggc
//
LOCUS       DAOOKS010000105         3719 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_30019_length_3719_cov_3.225164, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000105 DAOOKS010000000
VERSION     DAOOKS010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30019_length_3719_cov_3.225164"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..961)
                     /locus_tag="PK112_02555"
     CDS             complement(59..961)
                     /locus_tag="PK112_02555"
                     /inference="COORDINATES: protein motif:HMM:NF039187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HPC10962.1"
                     /translation="MTPARFRWGLFLVLLGLLLLLRNFGVLNDNFWLDLLILFPVVLI
                     MVGLEKIFARTRLSIISYLTSVALFVGGLAIAFTSSRGGDSTSFFSTATYVKDADSAV
                     RFTRAVLDLDESDLTIRDSGDDLVYAKFDKFTRKPRIDFREEDSLAVVEFASRPHGLL
                     GGAIKIETDEAPDWFVRFKRDVPLEMRLAGVKSDMHLNMSTTPLRALDLSADQSSVYL
                     KIGDLEPFVKVSVFGEDTELRLRVPRTVGLRVLAGEEYKAYLYSLGLITRDDAWVNEG
                     FDTLPNKIEVKLDDRLQSVSVDFF"
     gene            complement(958..1419)
                     /locus_tag="PK112_02560"
     CDS             complement(958..1419)
                     /locus_tag="PK112_02560"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HPC10963.1"
                     /translation="MSYTRLYRSAENKVIAGVCGGLGEYFDVDPNLVRVFAVLLFFAT
                     QGFATLAYAAAWIIVPKRPLEGAVEEVRPERPRSSWIRYLPGLVLILIGSLLLVRQHW
                     HWIDFDQWWPALMIAGGLFLLLRPRRRREACRPDLSAAPGEIPGGNEGRTA"
     gene            complement(1489..2766)
                     /locus_tag="PK112_02565"
     CDS             complement(1489..2766)
                     /locus_tag="PK112_02565"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HPC10964.1"
                     /translation="MDHGYFKDRVSGYLDKELPDYEMEALRRHLGECAECRALYEDLR
                     RLEALVERHADLGGDEDYWEAAAARIEKRIGIAGAPEVIALPPRRSRGLAWKLVGVAA
                     SIAALTFIAVNYTDIVRQRDESPKMSFPSEPPSARDSAAAGRAGEPSPAGEEQPAEKA
                     ERTAPPTAEPPEPAEIAALPELKRQAAEDEQLPVIAEEAQEAPRLKPAGQELAASGGK
                     AAAQDAEAEANRSAAAPAAPAAPPPEPVSAETRKDEGAADVRLADRLVLRDLMVAKEP
                     ATAAAPSLSLADSSAADRRGRLPLAQSSKGFAAAGESAPAATLADWRNRRDSLESLWS
                     ELRSEHLNLALPKARRDRAALGDIELVERQLLEAQVQVGRLAEGADAPERDRAIQFFR
                     TYLELADVRYRETARLYLDELAPDTVDTSGSRR"
     gene            complement(2790..3389)
                     /locus_tag="PK112_02570"
     CDS             complement(2790..3389)
                     /locus_tag="PK112_02570"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPC10965.1"
                     /translation="MGENSREENLPRPVSGLDKAAERALVEAAQNGDNKAFGRLIRLH
                     QKRLFRYIYAMLGSFDAAEDVVQEAFVKAYENLKSFKPEYDFYPWLSTIARNTAYNLV
                     RKEEKNESLNALAEKGFDPESGDPGPLEKLLAEEGEKRFYQALKTLPVQHRVAFVLRH
                     LEGMNYAEIASYLKIPPGTVDSRLYRARQMLMEALQDLL"
     gene            complement(3382..>3719)
                     /locus_tag="PK112_02575"
     CDS             complement(3382..>3719)
                     /locus_tag="PK112_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10966.1"
                     /translation="QIVTEQPHRVLRSLEQLIAEGNSPVTICTLLSTHFLRLYLVRHR
                     KPVPGNMAWLESRLRPQANRFDSARLEQILIDLAEAEAQLRGGGLPPRLALEILVVGL
                     MPGRDALYG"
BASE COUNT          619 a         1231 c         1231 g          638 t
ORIGIN      
        1 aacggcggcg ccccgaagcg ccgccgccat ctcaccgaca actggaggaa agaaccgatc
       61 agaagaaatc caccgacacc gactgcagcc ggtcgtcgag cttcacttca atcttgttgg
      121 gaagggtatc gaaaccctcg ttgacccagg catcgtccct ggtgatcagg ccgagcgagt
      181 agaggtaggc tttgtactcc tccccggcca gcactctcag cccgaccgtg cgcggcaccc
      241 gcaggcgcag ctcggtatcc tcgccgaaca ccgagacttt cacgaacggt tcgaggtcgc
      301 cgattttgag gtagaccgag gactggtcgg cggaaagatc aagcgcccgg aggggggtgg
      361 tcgacatgtt gaggtgcatg tcgctcttaa cgccggcgag gcgcatttcg agcggcacgt
      421 cccgcttgaa acgcacgaac cagtcggggg cctcgtcggt ctcgatcttg atcgcgccgc
      481 cgagcagccc gtgcgggcgg ctcgcgaatt ccaccaccgc gaggctgtcc tcctcgcgga
      541 agtcgatccg cggcttgcgc gtgaacttgt cgaacttcgc gtacacgagg tcatcccccg
      601 aatcccggat cgtcagatcc gactcatcga gatccagcac cgcccgcgtg aatctcacgg
      661 cgctgtccgc atccttcacg taggtcgcgg tcgaaaagaa gctcgtgctg tcgccgccgc
      721 ggctgctggt gaaagcgatc gccagcccgc ccacgaacag cgccaccgaa gtgaggtagg
      781 aaatgatgct gaggcgggtg cgggcgaaga tcttctcgag gccgaccatg ataagcacga
      841 ccgggaagag gatgagcagg tcgagccaga agttgtcgtt gaggacgccg aagttgcgca
      901 ggagcaggag cagtccgagc aggacgagga acagtcccca gcggaagcgg gccggtgtca
      961 tgccgtcctc ccttcattgc cgcccgggat ctcccccggc gcggcggaca gatccggccg
     1021 gcaggcttcg cggcgccgcc tggggcggag cagcagaaac aaaccgcccg cgatcatgag
     1081 ggccggccac cactggtcaa agtcgatcca gtgccagtgc tgccggacca atagcagcga
     1141 gccgatcagg atgaggacca gccctgggag atagcggatc cacgaggagc gcggccgctc
     1201 cgggcgaact tcctcgacgg cgccttcgag cggacgtttg ggaacgataa tccaggccgc
     1261 cgcataggcg agggtcgcga acccctgggt ggcgaaaaac aacagcaccg cgaacacccg
     1321 cacgaggttg gggtccacgt cgaagtattc gccgagaccg ccgcacacgc ccgcgatcac
     1381 cttgttctcc gccgaccgat agagtctggt gtagctcatg cgccatcgtc tcctttctac
     1441 cggccgcagc cggattccgg gatactatac gtgaatcggc ggaaaaggtt accggcgaga
     1501 accgctggtg tcgacggtat cgggggccaa ctcgtccaga tagaggcgcg ccgtctcccg
     1561 gtagcgcacg tcggccagtt cgaggtaggt gcggaagaac tggatcgccc ggtcgcgctc
     1621 cggagcatcc gctccctccg ccaggcggcc gacctgcacc tgggcctcga ggagctggcg
     1681 ctcgaccagt tcgatgtccc cgagcgccgc ccggtcgcgt cgcgccttgg gcagcgcgag
     1741 gttcagatgt tcgctccgca gttcgctcca gagcgactcg agcgaatcgc ggcggttccg
     1801 ccagtcggcg agagtggccg ccggagcgct ttcgccggcg gccgcgaacc ctttcgacga
     1861 ctgcgccagg ggcaggcgtc cgcgccgatc ggccgccgag gaatcggcga ggctgagact
     1921 gggcgccgcc gctgtcgccg gctccttggc gaccatgagg tcgcggagga ccagccggtc
     1981 ggcgaggcgg acgtcggccg cgccctcgtc tttgcgggtc tcggccgaga ccggttccgg
     2041 cggcggcgcg gccggggccg cgggagccgc cgcactccgg ttcgcctccg cctcggcatc
     2101 ttgcgcggcc gccttcccgc cggaggccgc aagctcctgg ccggccggtt tgaggcgcgg
     2161 cgcttcctgt gcttcctcgg cgataaccgg gagttgttcg tcctccgccg cctggcgctt
     2221 gagctcaggg agcgccgcaa tctcggccgg ctcgggcggc tccgcggtgg gcggcgcggt
     2281 tctctcggct ttctccgccg gctgctcttc gccggccggt gagggctccc cggctcggcc
     2341 ggccgcggct gagtcacggg ccgacggcgg ctcggacggg aaggacatct tcggcgactc
     2401 gtctctctgg cggacgatgt cggtgtagtt caccgcgata aatgtcagcg cggcgatcga
     2461 agctgccact ccgaccagct tccaggccag cccccggctc cggcgcggcg ggagggcgat
     2521 cacctccggt gcgccggcga tcccgatccg cttttcgatc cgggcggccg ccgcctccca
     2581 gtagtcctcg tccccgccga ggtcggcatg gcgctcgacc agcgcctcca gccgtcgcag
     2641 atcctcgtag agggcgcggc attcggcgca ctctccgagg tggcggcgga gcgcctccat
     2701 ctcgtagtcc gggagttcct tgtcgagata accggagact ctgtccttaa agtacccgtg
     2761 atccataatt caactgctgt accttgacct cagaggaggt cctggagcgc ctccatcaac
     2821 atctggcgcg cccggtagag ccgggaatcg accgtccccg gcggtatttt caggtagctg
     2881 gcgatctcgg cgtagttcat gccctcgagg tggcggagca cgaaggcgac gcggtgctgc
     2941 acggggagag ttttgagagc ctggtagaac cgtttctctc cttcttccgc cagcagtttt
     3001 tccagcggtc cggggtcgcc cgattcgggg tcgaaaccct tttcggctaa ggcattcagg
     3061 gactcgttct tttcctcctt gcggaccagg ttgtaggcgg tgttgcgggc gattgtagag
     3121 agccaggggt agaaatcgta ctcgggcttg aagcttttca ggttttcgta tgccttcaca
     3181 aacgcctcct ggacaacatc ttccgcggca tcgaacgacc ccagcatggc gtagatgtac
     3241 cggaacagcc gtttctggtg caggcggatg agccgtccaa acgccttatt gtcgccgttc
     3301 tgggccgcct cgaccaaggc ccgctcggcc gctttgtcca gaccggatac cggccgggga
     3361 aggttttctt cccgagaatt ttcacccata gagcgcgtcc cggcccggca tgaggccgac
     3421 cacgaggatt tccagggcca ggcgcggggg caaaccgccg ccccgcaatt gcgcctcggc
     3481 ctcggccaga tcgatcagaa tctgctccag ccgggcgctg tcgaaccgat tcgcctgcgg
     3541 ccgcaggcgg gactccagcc acgccatgtt ccccggcacc ggtttccggt ggcgtacgag
     3601 atagaggcgc aggaagtggg tgctcagaag cgtgcagatc gtcaccggcg aattcccctc
     3661 cgcgatcaac tgctccagac tgcggagcac ccggtgtggc tgttcggtga caatctggt
//
LOCUS       DAOOKS010000106         3716 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_30040_length_3716_cov_3.621142, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000106 DAOOKS010000000
VERSION     DAOOKS010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3716
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30040_length_3716_cov_3.621142"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(384..1895)
                     /locus_tag="PK112_02580"
     CDS             complement(384..1895)
                     /locus_tag="PK112_02580"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HPC10967.1"
                     /translation="MHPAPNTRPPAGRRLRFGRKVSALVLVLAVLAAAGAAALLAGCF
                     TNPATGKETFSLYSTGQEIQIGRESDQAIRAQLGVAGDSALQRYVAGIGRNLAAVSER
                     RDLPWTFCVVDDPTINAFALPGGYVYVTRGILAHMENEAELASVMGHEIGHITARHSV
                     TQMTKQQLAQLGLAGAVILKPDLEKYGQLISAGLGLLFLKFSRDDEHQADLLGLRYMY
                     RGGYDPREMDDVFAMLDRVSGQQGGSALPNWLSTHPSSADRIQRITRMVDSLPPDFSG
                     RKVSRNEFLQRIDGLVYGDNPREGYFRGNTFIHPDLKFHIDFPDQWQTANQKQSVAGV
                     SPNEDALLQVSLAEAQTPAAAAQQFVSQQGISASAPQGATIGGLSAATATFSAQTGDG
                     VQLTGTAAFVGYNGRVFEILGYGLAGQWSQYRSAVAGAIASFGPVTDQSLLNVKPRRI
                     EIVTIPRAMTIQEFNQAYPSDSKTTVEELTILNLVQSGEKIPAGTMVKRVVAK"
     gene            2206..>3716
                     /locus_tag="PK112_02585"
     CDS             2206..>3716
                     /locus_tag="PK112_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HPC10968.1"
                     /translation="MTGRDDQTRAPEIEYENREWLESLDYVHRSQGPGRVRQLLRRLQ
                     MRAQENGVHLPYTANTPYVNTIPVERQPVFPGSREIERRIKSIIRWNAMAMVVRANRD
                     HSGIGGHISTYASTATLYEVGFNHFFRARTENHPGDLVYFQGHAAPGVYARAFLEGRL
                     SEAQLANFRQELAPGGGLSSYPHPYLMPEFWQFPTVSMGLSPLSAIYQARFIRYLEDR
                     GLKRRDDQKVWAFLGDGELDEPESLGALTLPPRERLDNLIFVINCNLQRLDGPVRGNG
                     KIIQELEAAFRGAGWNVIKVIWGDDWDPLLAQDDKGLLVQRMEEVPDGQYQMYSVASG
                     DYIRRDFFGKYPELERMVRHYTDEQLQKLRRGGHDPHKVYAAYKQAVEHEGSPTVILA
                     KTIKGYGLGEAGEGRNVTHQQKKLNEQELRHFRSRFGIPLPDSSLAEAPFYRPAPDSE
                     EMRYLKERREALGGYLPKRLITVPPMPAPADDLYAEFLKGSGEGWVTAEYSMLP"
BASE COUNT          639 a         1256 c         1210 g          611 t
ORIGIN      
        1 cccggccgca caaatcaagg ataatcgatt atccggcatg tggttgccgg taatcacgcc
       61 cggtcggcgg aagcccggcg ggcggcggga ttgtcggttt tgcgccacac gttgatggac
      121 gtcgccgaca gcgagcagca cagcggcggg gtctggggag ggcgcttttg cgaagcgcgt
      181 tccgaggccg ccgagatcag aaagacaccg cacgcggcca acctccgcag gacaggggtt
      241 tggtaggtca ggagcttcat ggtcctgacg gcgccgtttg cgtcgttggc aagaatgtca
      301 ggaccacaag ggtcctgacc tacatggaca agaggccaat gcctcctccc agccgccgcc
      361 ttcccggtgc tgcttcccgg cctctacttc gccaccaccc gcttgaccat cgtccccgcc
      421 ggaatcttct cgccgctctg caccaggttg aggatggtca attcctccac cgtggttttg
      481 gaatccgacg gatacgcctg attgaactcc tggatcgtca tggcgcgcgg gatggtcacg
      541 atctcgatgc ggcggggctt aacgttcaga agggactggt cggtgacggg gccaaacgac
      601 gctatggcgc cggccaccgc gctccggtac tggctccact ggccggcgag gccgtagccg
      661 aggatttcga acacccgccc gttgtagccg acaaaggccg ccgtgccggt caactgcacg
      721 ccgtccccgg tctgcgccga gaaggtcgcg gtggcggcgc tgagtccgcc gatggtggcg
      781 ccctggggcg ccgaggcgct gattccctgc tggctcacga attgctgcgc ggcggcggcg
      841 ggcgtctggg cctccgcgag cgagacctgg agaagcgcat cctcgttggg tgacaccccg
      901 gccaccgact gcttctggtt ggctgtctgc cactggtcgg ggaaatcaat atggaatttc
      961 aggtcggggt ggatgaaggt gtttccccgg aaatatccct cgcgcgggtt gtcgccgtag
     1021 accaggccgt cgatccgttg gaggaactcg ttgcggctca ctttgcggcc gctgaaatcc
     1081 gggggcagcg aatccaccat ccgggtgatg cgctggatcc ggtcggccga cgagggatgg
     1141 gtcgacagcc agttcggcag cgcgcttccg ccctgctgcc cgctcacccg gtccagcatc
     1201 gcgaagacat cgtccatctc gcgcggatcg tagccccccc ggtacatgta gcggaggccg
     1261 aggagatcgg cctggtgctc gtcgtcgcgg ctgaacttca gaaacaggag tccgagaccg
     1321 gcggaaatca actgcccgta tttctcaaga tccggtttga ggatcacggc gccggccagt
     1381 ccgagctgcg ccaactgctg cttggtcatc tgcgtgaccg agtggcgggc ggtgatgtgg
     1441 ccgatctcgt gtcccatgac cgaggcgagc tcggcctcgt tctccatgtg ggcgagaatc
     1501 ccgcgcgtca cgtagacata cccgcccggg agcgcgaacg catttatggt cggatcgtcc
     1561 actacgcaga atgtccaggg caggtcccgc cgttcgctca cggcggcgag attccggccg
     1621 atccccgcca cgtaccgctg aagcgccgag tcccccgcca ctccgagctg cgcccggatt
     1681 gcctggtcgg attcgcggcc gatctggatt tcctggccgg tggaatagag cgagaacgtc
     1741 tctttgccgg tggccgggtt ggtgaaacag ccggccagca gggccgccgc cccggcagcg
     1801 gccaggacgg ccagcacgag gaccagggcc gagacctttc ggccaaaacg cagccgccga
     1861 ccagcgggcg gccgggtgtt cggcgcaggg tgcatggcgg aactcctttc gctattaagt
     1921 attcgcccta ggctacatcg gcgcccgcgg cccgtcaagc tttatggcgg ccggccggga
     1981 atgacgatta cgttgatcca gttaggtctc tctccgccgg aaacaacttg tgcccgcctc
     2041 cgttacgatg tatgtttggc caccgaggca gaacggcggt ccctggccga cgcccgcgga
     2101 cggcggcggc tccgggatcc ggggcgataa ttctcccccg catccaaccg cgcccgtcgc
     2161 acgggagcgg cgggaggcgg agcgagcatg cagaaaggcg ctgccatgac gggacgcgac
     2221 gaccagaccc gcgcgccgga gatcgaatac gagaaccggg agtggcttga gtccctggac
     2281 tacgtccacc gcagccaggg gccgggccgg gtccggcagc tgctccggcg gctgcagatg
     2341 cgggcgcagg aaaacggggt gcaccttccc tacacggcca acacccccta cgtcaacacg
     2401 atcccggtcg agcggcagcc ggttttcccc ggcagccgcg agatcgaacg gcggatcaag
     2461 agcattatcc ggtggaatgc gatggccatg gtggtgcggg ccaaccgcga ccacagcggc
     2521 atcggcggcc acatctccac ctacgcctcc accgccaccc tctacgaggt cgggttcaac
     2581 cacttcttcc gcgcccgcac cgagaaccat ccgggcgacc tcgtctattt tcaggggcac
     2641 gcggcgcccg gcgtctacgc ccgggcgttc ctcgaggggc gtctgagcga ggcccagctg
     2701 gccaacttcc gccaggagct ggcccccggg ggagggcttt cctcgtaccc ccacccctac
     2761 ctcatgccgg aattctggca gttcccgact gtgtccatgg gcctctcgcc gctctccgcc
     2821 atctaccagg cgcggttcat tcgctacctc gaggaccgcg gcttgaagcg ccgcgacgat
     2881 cagaaggtct gggcgtttct cggcgacggc gagctcgacg agccggagtc gctcggcgcc
     2941 ctcaccctcc cgccgcgcga aaggctcgac aacctcattt tcgtcatcaa ctgcaacctt
     3001 cagcggctcg acggcccggt gcgcggcaac gggaagatca tccaggaact ggaggcggcc
     3061 ttccgcggcg ccggctggaa cgtgatcaaa gtcatctggg gggacgactg ggacccgctc
     3121 ttggcgcagg acgacaaggg gctgctcgtg cagcgcatgg aggaggtgcc cgacggccag
     3181 taccagatgt actcggtggc ctccggcgat tacatccgcc gggatttttt cggcaaatac
     3241 cccgagctcg agcgcatggt gcggcactac accgacgagc agctccagaa actgcgccgc
     3301 ggcggccacg acccgcacaa ggtgtacgcc gcgtacaagc aggcggtcga acatgagggt
     3361 tcacccaccg tcattctcgc gaagaccatc aagggctacg ggctcggcga ggcgggcgag
     3421 ggacgcaacg tcacgcacca gcagaaaaaa ctcaacgaac aggagctgcg ccacttccgc
     3481 agccgtttcg gcatccccct gcccgattcc tcgctcgccg aagccccctt ctaccgcccc
     3541 gcccccgaca gcgaggagat gcgctacctc aaggagcgcc gcgaggccct cggcggctac
     3601 ctcccgaaac gcctcatcac ggtgccgccc atgcccgccc cggccgatga cctctatgcg
     3661 gagttcctca agggcagcgg cgaggggtgg gtaaccgccg agtactccat gctgcc
//
LOCUS       DAOOKS010000107         3665 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_30605_length_3665_cov_3.360665, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000107 DAOOKS010000000
VERSION     DAOOKS010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3665
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30605_length_3665_cov_3.360665"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="PK112_02590"
     CDS             <1..651
                     /locus_tag="PK112_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10969.1"
                     /translation="DRTEQVFTAAVEQPSAAAARFLRQVRLALERMSPAAAVRDITLR
                     IPHPAAAAAEQTAFAQPGARGEIDAETAATIRRTLSGNRLCTARLLPSHLPERAFRLS
                     PVTPSPAKRPSASSLSAGAAPLDRLPPYSLRRIAGLRLLQPPQPAEVHPADGWLRDGS
                     GKHRVTGRRGPWKLSGDWWQTGFDRLYYELQTEEHRQYLVFFDRSRARWFVQGVFD"
     gene            complement(751..1359)
                     /locus_tag="PK112_02595"
     CDS             complement(751..1359)
                     /locus_tag="PK112_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10970.1"
                     /translation="MDLLGQAPCGPDILAIDEDDPVRTIKVLTYKKNLLTEVSLKVIR
                     FYISPTLVIKSSLGEPCQGGVPVVERVAYEAVFDRVIMDIVYMALQICRTADDMIPVA
                     MLPQAALAHMMSSPIECGEILFHAMDDYIGLSIPRLQNSMPVLGLNYPRQYSVFQDHA
                     GSPDSGQQQIRAVEENGLPVVCNAGDEICPARFEETTQFRHR"
     gene            2252..2903
                     /locus_tag="PK112_02600"
                     /pseudo
     CDS             2252..2903
                     /locus_tag="PK112_02600"
                     /inference="COORDINATES: protein motif:HMM:NF019353.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
     gene            2970..>3665
                     /locus_tag="PK112_02605"
     CDS             2970..>3665
                     /locus_tag="PK112_02605"
                     /inference="COORDINATES: protein motif:HMM:NF036968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HPC10971.1"
                     /translation="MLTLIDLALKLVKEHRGVTIDPARLSYDDPKVFDLLCRAETLGV
                     FQVESRAQMNALPRHRPRCFYDLVIEVALIRPGPIQGDMVHPYLRRRNGEEPVTYPHP
                     CLEPILRRTLGVPLFQEQGMKVAVAAAGFTASQADELRRAMGHKRSRERMAALAERLI
                     DGMQRHGIPHDAARRIFDQLAAFADFGFAESHAASFALLVYVSAYLKVYYPQEFYCSL
                     LNAQPMGFYAPSSL"
BASE COUNT          598 a         1247 c         1159 g          661 t
ORIGIN      
        1 gaccgcaccg agcaggtttt caccgcggcg gtcgaacaac cgtcggccgc cgccgcccgc
       61 ttcctgcgcc aggtgcgcct ggcgctcgaa cggatgtcgc cggccgccgc ggtgcgcgac
      121 atcaccctcc gcattccgca ccccgccgcg gcggccgcgg agcagaccgc gttcgcgcag
      181 ccgggcgcgc ggggcgagat cgatgcggag acggccgcga caatccggcg cacgctgagc
      241 gggaatcgcc tgtgcacggc gcgcctgctg ccgtcgcacc tgccggagcg ggcgttccgc
      301 ctctcccccg tgacgccgtc gccggccaaa cgcccctccg cctcttcgtt gtccgccggc
      361 gccgcacccc tcgatcgcct tcccccgtac agcctgcgcc ggatcgccgg actccggctg
      421 ttgcagccgc cccagccggc cgaggtgcat ccggcggatg gttggttgcg cgatggttcg
      481 gggaagcatc gggtcaccgg acggcggggg ccgtggaaac tgtcgggcga ctggtggcag
      541 accggttttg accgcttgta ctacgagcta cagacggagg agcaccggca gtacctcgtt
      601 tttttcgacc gaagccgggc gcggtggttt gtgcaggggg tgtttgattg atggggagaa
      661 atggcaggtc agggccttta tggtcctgac gggtgaatta tcgaggatag gaggaatgtc
      721 aggaccacaa gggtcctgac ctacaagaaa tcacctatgt cgaaactgcg tcgtttcttc
      781 gaacctggcc ggacatattt catcacctgc gttacataca accggcagcc cattctcctc
      841 gactgcgcgg atttgctgct gtccgctatc aggagaaccc gcatggtcct gaaataccga
      901 atactggcgt gggtaattca acccgagcac tggcatgcta ttctgcaacc gcggaatact
      961 cagcccgatg taatcatcca tcgcatgaaa cagaatttct ccacactcta tcgggctcga
     1021 catcatgtgc gccagggccg cttgtggcag catcgctact gggatcatgt catccgcagt
     1081 ccggcagatc tgcaacgcca tgtagactat atccattata acccggtcaa acacggcctc
     1141 gtaggcaacc ctttcgacta ccggcactcc tccctggcat ggttcgccga gcgaggattt
     1201 tatgacgagg gttggggaaa tgtaaaaccg gataaccttg agggagactt cggtgagtag
     1261 atttttcttg taggtcagga cctttatggt cctgacgggg tcgtcctcat cgatggcaag
     1321 aatgtcagga ccacaagggg cctgaccgag aagatccatg acctccgttc cctacatcga
     1381 actccactgc cactcgaact tttcgttcct cgacggcgcc agcgacccgg aggcgctggt
     1441 cgaccgggcg gtcgaactcg gctatcccgc gctggcgatc accgacacca acggtctgta
     1501 cggtattgtc cgttttgacc agcaggcgcg ccgggcgggg ctccgtccga ttttcggggc
     1561 ggccgcgctt ttggacaccg ggcaccggct ggtgctgctg gtcaaagacg gcgccggcta
     1621 cgccaacctc tccgagctgc tctccaccgc ccagttggag agccccaagg gggaggcacg
     1681 ggtgtcgcgc gctctgctgg agcggcacgc cgacggtctc atcgcgctcg tccacgatga
     1741 cctcgcggcg tgtctcttgc gggaggagca ctcggaggcg gaacggctgc tcgccgacta
     1801 ccgcgacctg tacgggcgcg accacgtgta tgtcgagctt cgccatcaca acctgccggt
     1861 gcacgagcac ctctgcgccg cgctggtgcg gctgggacgg cggctcggcc ttcccgcggt
     1921 cgccgccaac gatgtccact acgccgcccc cgacggccgc cggctgcagg acgtgctttc
     1981 ctgtatccgc caccacacca cgctcgacga ggccggcgag ctgctctacc cgaatgcgga
     2041 gcggtgcctc cagccgccgg cggtgatggc gcgccggttc gcccgctacc ccgaggcggc
     2101 ggcccacacc ctcgcgatcg ccgagcgctg ctggttctcg ctcgagcagc tcgaaacctc
     2161 gctgcccgat ttcccggttc cggcggggca caccgagcag agctacctgg aggaactgac
     2221 ctgggccggg gcgcggcggc gctacccgga gatggcggag ccgcatgtcc gccagcttag
     2281 acacgagctg cgcatcatcg accggctcaa gctggccggc tacttcctca tcgtctggga
     2341 tatcgcccgt ttctgcgccg agcgcgccat tctctgccaa gggcggggct cggccgccaa
     2401 ctcggcggtc tgctactgcc tcgggatcac ggcggtcgat ccgatcaagc tacctgctgt
     2461 tcgagcgctt catttccgag gaacgccgcg agccgcccga catcgacatc gacatcgcca
     2521 acaaccgccg cgaggaggtc atccagtacg tgtatgccaa gtacgggcgg acgcacgcgg
     2581 cgatggtatg cgaggtgatc agctatcggg gacggtcggc ggtgcgcgat atcggcaaga
     2641 cgctcgggtt ttctccggac gaggtcaccc gcctgtccaa actgctcgac cactgggcct
     2701 cgccggagga tgtcgcggag cggctgcgcg aggcggcgct caatcttggc gaccgccgcg
     2761 tccgcctgct gctggacctc tgccgtcaga ttcaccgttt cccccgccac ctcgggattc
     2821 atgtcggcgg catgatcatc acccgcaagc ccctctcgca ggtggtgccg atcgagaatg
     2881 cgacgatgcc cgaccggagc gtgatccagt gggacaagga cgacgtcggt gcggccgggc
     2941 tggtcaagat cgacctgctc gggctgggca tgctcaccct gatcgacctc gcgctcaagc
     3001 tggtgaaaga gcaccgcggc gtcacgatcg acccggcccg gctctcctac gatgatccga
     3061 aagttttcga cctcctctgc cgggcggaga cgctcggcgt gttccaggtc gagtcgcgcg
     3121 cgcagatgaa cgcccttccc cgccaccggc cgcgctgttt ttacgatctc gtgatcgaag
     3181 tggcgctcat ccgtcccggc ccgatccagg gggacatggt ccacccctac ctgcgccgcc
     3241 gcaacggcga ggagccggtc acctatccgc acccgtgcct tgagccgatt ctccggcgca
     3301 ctctcggggt gccgcttttt caggagcagg ggatgaaggt cgcggtagcg gcggccgggt
     3361 tcacggccag ccaggcggac gaacttcgcc gcgcgatggg gcacaagcgc tcgcgcgagc
     3421 ggatggcggc gctggcggag cggctcatcg acgggatgca gcggcacggc attccccacg
     3481 acgccgcccg gcggattttc gaccaactgg ccgccttcgc cgatttcggc tttgccgagt
     3541 cgcacgccgc ctccttcgcc ctgctcgtgt acgtctccgc gtatctgaaa gtgtattacc
     3601 cccaggaatt ttactgctcg ctgctcaacg cccagccgat gggcttctac gcgccgtcga
     3661 gcctc
//
LOCUS       DAOOKS010000108         3663 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_30620_length_3663_cov_2.909368, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000108 DAOOKS010000000
VERSION     DAOOKS010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30620_length_3663_cov_2.909368"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /locus_tag="PK112_02610"
     CDS             complement(<1..843)
                     /locus_tag="PK112_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10972.1"
                     /translation="MRTLIAAAVFHLLAACAAFPAFAEGILTITTYGTGSEGHLITFP
                     DSTTMSLDCADGDITTMSHFHSDHCGSSCESSAQYHRNNTKPGDILYDKDGVIVECVA
                     SNQKVISTGWKTAGCKDVDENAYSMALLVSYGGFDYLFTGDMYGATEVPLGEALWAAG
                     VGVDILKASHHGTDTNNSSSVEYLESISPEYVVIAGTATSPNATSLAAFKSAGVTRIY
                     YAPNYTSSNDIVFRANGDITVTTDGETFSISGSSFNHGPYWADEASATPTPPPTRTPT
                     RTPPR"
     gene            complement(1099..2427)
                     /locus_tag="PK112_02615"
     CDS             complement(1099..2427)
                     /locus_tag="PK112_02615"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HPC10973.1"
                     /translation="MSWNVSKLTTKAGIETVRIIQGLQPDVACLQEWYVDYPGDYDFD
                     GWVSTAFGAEFVYHRASSGYQPNGIVSRWDILSSGSWPDLQRANYYHDWAVIDLPGAT
                     NLQVVSVHLHSSDEAIRKAQIKDVIDYIETNFSNSHYLMLGGDFNTANRTSEPIPTLL
                     NSTNWSGSCWVTTSDGTPTDQAGGDDDTNVNRTYNYDWLIPNTLLGAEIMNLDLGEDS
                     GAYAGGIVFDSRVFTPLSAVAPAQYGDTANGDQDHCPVMKSYDVLPSENLIDEGFEGG
                     TYPPTSWTHNGTTQSPTAPHGGTYSALINANADYLITPQLTTPGTMTFWYKGTSGSMN
                     VERATNLGGPWTAVTSPFAGSGSWEQKSVDLSAYSNIYIRFTRVPSAGNHYIDDLLVT
                     LGGGAPPSSGNTVKINFQPSGADPPTGDWMRDWGSGNNGLSHVPPYSFSW"
     gene            complement(2715..>3663)
                     /locus_tag="PK112_02620"
     CDS             complement(2715..>3663)
                     /locus_tag="PK112_02620"
                     /inference="COORDINATES: protein motif:HMM:NF039815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10974.1"
                     /translation="NAGFGRGTAIGVGSPIPLGEGAYAACVAANGLFIDGAYVAPSAS
                     DPNSRSVALLIEYGDFDYLTAGDMPGTREDELACALVSYANPSGHPYHSDAPYLSAES
                     GVDVLHANHHGSKTSSLACYLNAMKPEIALISGGTAYNHPTQDAVDRLLGRAVYSSCA
                     SLAGRPTGVTAAGALVYRTTASGQECPCARETDCPSVGDIVITTDGQSAYAVQTSELD
                     PVRIALDEGFLNVDSDGDGLTNADEEGFWHTDPLLPDTDGDGYTDYMEAMCGSEPNLP
                     GSTPVLRVSFQPGDADAPSDAMPAAVEDWRTGAGFGWAR"
BASE COUNT          623 a         1097 c         1256 g          687 t
ORIGIN      
        1 ccgggggggg gtgcgggtcg gcgtccgggt gggggggggc gtgggggtcg ctgaggcctc
       61 gtccgcccaa taggggccgt ggttgaacga ggacccggaa atggagaagg tctcgccgtc
      121 ggtggtgacc gtgatgtcgc cgttcgcccg gaagacgatg tcgttggacg acgtgtagtt
      181 cggggcgtag tagatcctcg taacccccgc cgacttgaat gcggccagcg aggtcgcgtt
      241 ggggctcgtg gcggttccgg cgatgacgac gtactcgggc gagatggact cgaggtactc
      301 cacgctcgat gagttgttgg tgtcggtgcc gtggtggctt gccttgagga tatccacgcc
      361 gacccccgcc gcccagagcg cctcgcccag gggcacctcg gtggcgccat acatatcccc
      421 cgtgaacagg tagtcgaagc cgccgtagct gaccagcaac gccatgctgt acgcgttttc
      481 gtcgacgtct ttgcaccccg ccgtcttcca cccggtcgaa atgaccttct ggttggaggc
      541 gacgcactcc acgatcacgc cgtccttgtc gtagaggata tccccgggct tggtgttgtt
      601 ccggtggtac tgtgcgctcg attcacagga actgccgcaa tggtcggagt gaaaatgcga
      661 catggtggtg atgtccccgt cggcgcagtc gagcgacatc gtcgtcgagt cggggaaggt
      721 gatgagatgc ccctcgctgc ctgtgccgta ggtggtgatc gtcaggatcc cctcggcgaa
      781 cgccgggaat gcggcacagg cggccaggag gtgaaaaacc gcggctgcaa tgagcgtgcg
      841 cataccgccc tgcctcttct acggatgaac gcggccaccg tgcggggggc acaacccccg
      901 ctatttgtag agtaatatac cccaggaagg gtgaggatgc aaacaattat atgcgccgtt
      961 cgtcaaaaat cgaaagaact cccgtgcccg ttccgcgtga agcgggtccg tgcactgcgc
     1021 cgaatgacgg acgagacgac cggctggcga ttcgagatgc gcaggggccg gcgcgcaagc
     1081 ggcgggaaac ggcggcgtct accaggagaa gctgtacggc gggacgtggg acaagccgtt
     1141 gttgccgctg ccccagtcgc gcatccagtc gcctgtcggg ggatcggcgc cagacggctg
     1201 gaagttgatt ttcacggtgt tccctgacga cggcggcgcg ccgcccccca gtgtgacgag
     1261 gaggtcgtca atatagtggt ttcccgcaga ggggacgcga gtgaatcgaa tgtagatgtt
     1321 ggagtaggcc gaaaggtcta cgctcttttg ttcccacgaa ccagatcccg caaacgggct
     1381 tgtgacggcg gtccatggcc cgccgagatt ggtggcgcgt tccacgttca tgctcccgct
     1441 ggttcccttg taccagaagg tcatggtccc cggtgtggtg agctgcggag taatgaggta
     1501 atctgcattc gcgtttataa gcgcgctgta ggtgccgcca tggggagcgg taggtgattg
     1561 agttgttcca ttatgagtcc aactggtcgg cggataggtc ccgccctcaa aaccctcatc
     1621 gattaggttt tccgaaggaa gaacgtcata tgacttcatc accgggcagt gatcctggtc
     1681 gccgttggcg gtatcgccgt actgcgccgg agcgaccgcg gagaggggcg tgaagacacg
     1741 gctgtcgaag acgatgccgc cggcgtaggc gccgctgtcc tcgccgagat cgaggttcat
     1801 gatctcggcg cccaaaagtg tgttggggat aagccaatca taattgtagg tgcgattcac
     1861 gttggtgtcg tcgtcgcccc cggcctggtc ggtcggcgtg ccgtccgagg tggtgaccca
     1921 gcacgagccg gaccagttcg tgctgttaag gagggtgggg atcggctccg aggtccggtt
     1981 ggcggtgttg aagtcgccgc cgagcatcag gtagtggctg ttggagaagt tggtctcgat
     2041 atagtcgatg acatccttga tctgcgcctt ccggatcgcc tcatccgagg agtggagatg
     2101 cacgctgacg acctgcaggt tggtggcgcc cgggagatcg atgaccgccc agtcgtggta
     2161 gtagttcgcc ctctggaggt ccggccagct cccggaggag aggatatccc acctgctgac
     2221 aatgccgttg ggctggtagc cggacgaagc ccgatggtag acgaactccg ccccgaacgc
     2281 ggtgctgacc cagccgtcga agtcgtagtc acccgggtag tcgacgtacc attcctggag
     2341 gcacgccacg tcgggctgta gcccctggat gatccgcacc gtttctatcc cggccttcgt
     2401 cgtgagcttg ctgacattcc agctcatcac gcggagttcg tcggcgcgcg cgaccgaacc
     2461 gcctggggac gccgtcgaga ggaggaaaca acagacggcg agggttaaac accgggtgca
     2521 gcgacctggc gacagcatgg cacgaaaccc ccatcacccg tgtgccgcgc gggctaattg
     2581 ccgcacggta cattcgtcga ccccgggcga gacctcccaa ttcatgatac tgtctaaaga
     2641 cggagggcgc aacaggaaat tttcggcagg aaattttcgg cgacccgacg attcccacgc
     2701 ccccggaggg gacctcaacg cgcccagccg aagcccgctc ccgtcctcca atcctccacc
     2761 gcggcgggca tcgcatctga aggggcgtcc gcgtctcccg gctggaagct gaccctgaga
     2821 acgggtgtcg agccgggaag gttcggctcg ctcccgcaca tcgcctccat gtaatcggta
     2881 tagccgtccc cgtcggtgtc ggggagaagc gggtcggtat gccagaaccc ctcctcatcg
     2941 gcgttggtga gcccgtcgcc gtcgctgtcg acgttgagga acccctcgtc gagcgctatc
     3001 cgcaccgggt ccagttcgga tgtctgcacg gcataggcgg actggccgtc ggtggtgatg
     3061 acgatgtcgc cgacactcgg gcagtcggtt tcccgcgcac aggggcactc ctgcccgctc
     3121 gccgtggtgc ggtagacgag cgcccccgcg gcggtgacgc ccgtcggccg tcccgccagg
     3181 cttgcgcagg acgagtagac ggccctgccg agaaggcggt ccacggcgtc ctgggtcggg
     3241 tggttgtacg cggtgccgcc gctgatgagg gcgatctccg gcttcatcgc gttcagatag
     3301 caggccaggg agcttgtctt gcttccgtgg tggttggcat ggaggacgtc gacaccgctc
     3361 tccgcgctca ggtagggagc gtcggaatgg tacggatgcc ccgacggatt ggcgtatgag
     3421 acgagggcgc aggccagctc gtcctcccgg gtgcccggca tgtcgccggc ggtcaggtag
     3481 tcgaagtcgc cgtactcgat cagcagtgcg acgctccggc tgttggggtc gctcgcgctt
     3541 ggggcgacgt acgcgccgtc gatgaacagg ccgttcgccg ccacgcaggc ggcgtatgcc
     3601 ccctcgccga gcgggatggg ggatcccacc ccgatcgccg ttccccgccc gaatccagcg
     3661 ttc
//
LOCUS       DAOOKS010000109         3658 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_30669_length_3658_cov_3.624757, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000109 DAOOKS010000000
VERSION     DAOOKS010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3658
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30669_length_3658_cov_3.624757"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            34..285
                     /locus_tag="PK112_02625"
     CDS             34..285
                     /locus_tag="PK112_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10975.1"
                     /translation="MASRISCHAPSSVAGKSSIPATDDGAPGITTAEDHRRLSGPLLE
                     SVRDADAFRLRLAGRLAGEYVLRPVTIDGERRHLFYRCT"
     gene            345..653
                     /locus_tag="PK112_02630"
     CDS             345..653
                     /locus_tag="PK112_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10976.1"
                     /translation="MPNAHGPGFNLLGPVINIVTIIMGVVTAYFVTIQSLKIDLAAKA
                     ERAVVETLDKRLTAFEVSLTEGAVTRDQFYEFARDTEARLARIELYLAQQSEDERGNK
                     "
     gene            640..1023
                     /locus_tag="PK112_02635"
     CDS             640..1023
                     /locus_tag="PK112_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10977.1"
                     /translation="METNETLIEQVDDLAAAWTPDNLCELDFDRIRDLLGRHRRLLAE
                     HESLREDLAALRQDYVARIGGMAKAIAAVTRRTGAAQAHDAAAAVLEYLRTLETMTAA
                     QLIEQYRLAGARFRDAFPTSFGRLP"
     gene            1166..1585
                     /locus_tag="PK112_02640"
     CDS             1166..1585
                     /locus_tag="PK112_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10978.1"
                     /translation="MTIRPQNLEAVAPVLATFAVHQTGGVDDLKDAHLGEPVMLTGSN
                     EAGPISNGGQLLGKLVALTLADGDDGSRLATVQIGGVCRLKISATYPAAGNRVVGGTA
                     GTVKQAPAVSGDPAGGNIARGTVLAVNGAVDCTFLLN"
     gene            1597..2700
                     /locus_tag="PK112_02645"
     CDS             1597..2700
                     /locus_tag="PK112_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10979.1"
                     /translation="MTFEQFLRSAALTGAPRECDREWAGGAVDLSRTAGIEITRALYG
                     EAEARGLSLSELLELDDYDPSPAGAPLDAFERQLALAGIRFGGKTPTTVEQFYQQAPA
                     LLPEFMRREIRAGQAMRPELRRLIANTAIIASNTYTPFFIDTTDAACFSLRPVAEGAE
                     TPQVLVTEQNHAVRIADYGLALKASYKALRYRTASQFRILLWYIGFRLQTDKIGMLVE
                     TILNGDGNDNAAAVITSAASGVLAYDDLVNLWSQFAPFEMNTIICGVNLMKEILTLDE
                     FKDPMAGYRFQNTGDLFSPLGASLVRCDELAADLIVGLDSRFAIEEVVTQPLTIEYDK
                     IIEQRFEEAVISESVAYAKVIHGAAVVLDTVHA"
     gene            2838..>3658
                     /locus_tag="PK112_02650"
     CDS             2838..>3658
                     /locus_tag="PK112_02650"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HPC10980.1"
                     /translation="MRQTICPSTALHPLGAPPGRKLLRVSSGPHAGREAVLFQAAPGE
                     IACAWADRPYAGWSPPAAVTAQAEDSPFDAAIMPDGTIHLVYTEAGTGYLAARKLTLT
                     GGAWVPGPPVTVVDRPIARYPSLAILPDGALWVCWTGIESGTHRLFAKSSSDGGTTWG
                     AGPSDPGESLTLGASSAFGRLAVGPSDLYAAYTNGGMQLCVRSRPLGGGAWGTSTNLA
                     AGENFDEHFDVAAGVDGRLGVVWDNGALRYREFDGDQWAPTVVLDGAGGSWPQLV"
BASE COUNT          574 a         1307 c         1246 g          531 t
ORIGIN      
        1 acaatgatca gggcgtagta gtcagacgcg tcgatggcgt ctcgtattag ctgccatgcc
       61 ccgtcgtcag tcgctgggaa gagctccatc ccggcgactg acgacggggc gcccgggatc
      121 acgaccgccg aggaccaccg gcgcctgagc ggaccgctgc ttgagtcggt gcgggatgcc
      181 gacgccttcc gcctccgcct ggcgggccgt ctggcgggcg agtacgtgtt gcggccggtg
      241 acgatcgacg gggagcggcg gcacctgttt taccggtgca cgtagccgca aggaggcggc
      301 gcacctgaca aatctgcgac tcctgagaac caacggaggt gcatatgccc aacgcgcatg
      361 gccccgggtt caacctgctc gggccggtca tcaacatcgt caccatcatc atgggcgtgg
      421 tcaccgccta tttcgtgacg atccagtcgc tcaaaatcga cctggcggcc aaagctgaac
      481 gggcggtcgt ggaaacgctc gacaaacggc tgaccgcgtt cgaggtgagc ctgaccgagg
      541 gagcggtgac gcgcgatcag ttttacgaat tcgcccggga caccgaggcg cgcctggcgc
      601 gcatcgagct gtacctggcc caacaatcgg aggacgagcg tggaaacaaa tgaaaccctg
      661 atcgagcagg tcgatgacct cgcggccgcc tggacgccgg acaatctgtg cgagctcgat
      721 ttcgaccgca tccgcgacct gcttgggcgg caccgccggc tgctggcgga acacgagtcg
      781 ctccgggagg atctcgccgc cctgcggcag gactatgtcg cccggatcgg gggcatggcg
      841 aaagcgattg cggcggtcac ccgccggacc ggcgcggcgc aggcgcacga tgccgcagcg
      901 gcagttctcg agtatcttcg gacgctcgag acgatgaccg cggcgcagtt gatcgagcag
      961 taccgcctgg ccggcgcgcg tttccgcgat gctttcccga ccagtttcgg ccgcctgccg
     1021 tgagtgagac ggcggcgtgg cgccgccgcg cgcccggccg ccgctctgtc ccgtgggact
     1081 gaccgtcccg gccgcgcggg agcacccgcc tcccggccac cctgccacca agaccgatcg
     1141 taacccccac tccgtgagga gacctatgac gattcgaccg cagaaccttg aagccgtcgc
     1201 gccggtgctg gcgactttcg cagtccacca gaccggcggc gtcgacgatc tcaaagacgc
     1261 ccacctcggt gagccggtga tgctgaccgg cagcaacgag gcgggcccga tcagcaacgg
     1321 cggccagctg ctcgggaagc tggttgcgct gacactcgcc gacggcgacg acgggagccg
     1381 cctggcgacc gtgcagatcg gcggcgtctg ccgcctgaag atctccgcca cttacccggc
     1441 ggcgggcaac cgcgtggtcg gcgggaccgc cggcacggtc aagcaggcgc cggccgtctc
     1501 gggcgacccg gccggcggca acatcgcgcg cggcaccgtg ctcgccgtca acggcgctgt
     1561 cgactgcacc tttctcttga actgaaggag accgctatga cgttcgaaca gtttcttcgc
     1621 tcggcggcgc tgaccggcgc gccgcgggaa tgcgaccgcg agtgggccgg aggcgccgtc
     1681 gacctgagcc ggacggccgg gatcgagatc acccgcgcgc tctacggcga ggcggaggcc
     1741 cgcggcctga gcctgtcgga gttgctcgag ctcgacgact acgacccgag ccccgccggg
     1801 gcgccgctcg acgcattcga gcgccagctc gcgctggccg ggatccgctt cggcggcaag
     1861 actccgacca cggtcgagca gttctaccag caggcgccgg cgctccttcc ggagttcatg
     1921 cgccgggaga tccgcgccgg gcaggcgatg cgccccgaac tccggcgcct catcgccaac
     1981 accgcgatca tcgcctccaa cacctacacc ccgttcttca tcgacacgac cgacgccgcc
     2041 tgcttctcgc tccggccggt ggccgagggc gcggagacgc cgcaggtgct ggtcaccgag
     2101 cagaaccacg ccgtccgcat cgccgactac ggcctggccc tgaaagccag ctacaaggcg
     2161 ctccgctacc gcacggcctc gcagttccgc atcctcctct ggtacatcgg gttccgcctc
     2221 cagacggaca agatcgggat gctggtggag acgattctca acggcgacgg caacgacaac
     2281 gccgccgcgg tcatcacctc ggccgccagc ggcgtgctgg cctacgacga cctggtcaac
     2341 ctctggtccc agttcgcgcc gttcgagatg aacacgatca tctgcggtgt caacctgatg
     2401 aaggagatcc tcaccctcga cgagttcaag gacccgatgg ccggctaccg cttccagaac
     2461 acgggggacc tgttcagccc gctgggggcg tcgctggtgc gctgcgacga actggccgcc
     2521 gacctgattg tcggcctcga cagccgcttc gcgatcgagg aggtcgtgac tcagccgctg
     2581 acgatcgagt acgacaagat catcgagcag cgctttgagg aggccgtgat cagcgagtcg
     2641 gtcgcctatg cgaaggtgat ccacggcgcg gcggtcgtgc tcgataccgt tcacgcttaa
     2701 ccaccggggc cggcggcgcc gggcgaaggg gcgggctgca gcccgcgccg gacgcccgca
     2761 ccacccgccg gcgcggcgga cggccggggc cggggcgcac ctcccccccc ggccgcgccc
     2821 catgggaaag gagccacatg cgccagacta tctgtccttc caccgccctc cacccgctcg
     2881 gcgctccgcc ggggcggaaa ctgctgcgcg tttcgtccgg cccgcacgcc gggcgggagg
     2941 cggtgctctt ccaggccgcc ccgggcgaga tcgcctgcgc ctgggccgac cgcccctacg
     3001 ccggctggtc gccgcccgcc gccgtcaccg cccaggccga ggactctccg ttcgacgcgg
     3061 cgatcatgcc ggacgggacg attcacctcg tgtacaccga ggcaggcacc gggtatctcg
     3121 ccgcaaggaa gctcacgctc accggcggag cgtgggtccc cggtcccccg gtcacagtcg
     3181 tcgaccggcc gatcgcccgc tacccgagcc tcgcgatcct ccccgacggc gcgctctggg
     3241 tctgttggac ggggatcgag agcggtaccc accggttgtt tgcgaagtcc tcgagcgatg
     3301 gcgggacaac ctggggcgcc ggtccgtctg acccggggga gtcgctcacg ctgggagcat
     3361 cctcggcctt tggccggctg gccgtcggac cgagcgacct gtacgcggcg tacaccaacg
     3421 gcggcatgca gctgtgcgtg cggtcgcggc cgctcggcgg cggcgcctgg ggcacgagca
     3481 ccaacctcgc cgccggcgag aacttcgacg agcacttcga cgtcgccgcc ggcgtcgacg
     3541 gccggctcgg tgtcgtctgg gacaacggcg cgctccgcta ccgggagttc gacggcgacc
     3601 agtgggcgcc gacggtcgtg ctcgacggcg cggggggatc gtggccgcaa ctggtctt
//
LOCUS       DAOOKS010000110         3657 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_30690_length_3657_cov_2.694336, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000110 DAOOKS010000000
VERSION     DAOOKS010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3657
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30690_length_3657_cov_2.694336"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1364)
                     /locus_tag="PK112_02655"
     CDS             complement(<1..1364)
                     /locus_tag="PK112_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10981.1"
                     /translation="MASRSPAYCMTLHNVGRLVLAVNNNGTFGSEFSVPSDAPDCFTG
                     QTPPPGGCQYPKGTGAMYMFSAAFWIGAVLGRDTLVSTGSEGWSDVRQFFPNDEPFGN
                     TVRRSIIDPESEEYVNAVSEQDFIALYTDTSTILADAEEDGTAHRPLNIQVTQRSYAW
                     SYDYADDIILFDYEIKNIGNIDLRNVYMGVYVDADVCDNCQDNEGFADDICGFLVIYP
                     REYGRMRCEYNDTVYIAWIADNDGDFNAGEGIQSTPHVTGTRIVRTPADTLDVSFNWW
                     VSNGNAALDFGPREKDSVGRWKEPYRDFRTGGTGTPVGDRNRYYSLRNREFDYDQIFT
                     NTIREGDPSWIKPPSALAPDIADGFDTRYLLSFGPFNIRKGQKLPLSFAYVAGADLHR
                     DPDNGDNLLADPVLYYSNLYFDSLAENATWASWIYDNPGVDSDSDGYSGKFRCMATLP
                     DGSR"
     gene            1897..2796
                     /locus_tag="PK112_02660"
     CDS             1897..2796
                     /locus_tag="PK112_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007783884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HPC10982.1"
                     /translation="MTEFPVSGVETWWWLPSLVAFCVSTLTSTGGLSGAFLMLPFQVS
                     VLGFTGPGVTPTNLLYNVVGIPSGVYRYGREKRMLWPLAWAIVIGTMPGMAIGTVIRV
                     TLLPDPSAFKVFAGLVLAFVGVRLLRDVVRGEGARPSPRGNGAGRFEVSGGRLTKLTV
                     EYDFGRQRYTASTPKILLLSFLVGVAGGAYGIGGGAVIAPFLVSAFGLPVYTVAGAAL
                     FGTFVSSAAGVAMFWLIDLSGLTGSTAAQPDWLLGLALGIGGAFGTYTGARLQKRVPA
                     RGIKALLTAFLLFVAGRYIVEAF"
     gene            2814..3254
                     /locus_tag="PK112_02665"
     CDS             2814..3254
                     /locus_tag="PK112_02665"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HPC10983.1"
                     /translation="MDDTIAKFASPAPIAVVGATDETRKFGGYLYRELKRQGYRVYPV
                     NARRAEVDGDRAYRSLRDIPDDVEGAVVAIRPDRAGEVVADALARGIRRIWFQQGADF
                     TEAARTAREAGMETVVGKCILMYAGKARGVHRFHAVLARLFGRY"
     gene            3275..>3657
                     /locus_tag="PK112_02670"
     CDS             3275..>3657
                     /locus_tag="PK112_02670"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPC10984.1"
                     /translation="MATEIREIEDRDLDAFIRLQIDAYPGLGMIYERDREKLRQRIAE
                     RRQDVRVTLYGSYRGNRLVGCMRVHDYRMNFRGALVAAGGLGGVAVDLTVKREGVAGG
                     MVRFYLDKYAAAGAPMAVLWPFRPD"
BASE COUNT          639 a         1033 c         1303 g          682 t
ORIGIN      
        1 atgcgagacc cgtcgggcag ggtcgccatg cagcgaaact ttcccgaata accgtcgctg
       61 tcgctgtcga cgccggggtt gtcataaatc caggaggccc aggtggcgtt ttcggccagc
      121 gaatcgaaat agaggttgga gtagtagaga accggatcgg ccaggagatt gtcgccgtta
      181 tcgggatccc gatggagatc ggccccggcc acataggcga atgaaagcgg cagcttctgt
      241 cccttgcgaa tgttgaacgg tccgaaggag agcaggtagc gggtatcgaa tccgtcggcg
      301 atgtccggcg cgagcgccga gggcggctta atccaactgg ggtcgccctc gcggatcgtg
      361 ttggtaaaga tctggtcgta gtcgaactcg cggttccgga gcgagtagta gcggttgcgg
      421 tcgccgaccg gggtgccggt gccgccggtc cggaagtcac ggtagggttc tttccagcgt
      481 ccgaccgagt ccttctcgcg cgggccgaaa tcgagcgcgg cattgccgtt gctcacccac
      541 cagttgaaag agacatcgag cgtgtcggcg ggggtgcgga cgatgcgggt gccggtgacg
      601 tgcggcgtgg actgaatccc ctcgccggcg ttgaagtcgc cgtcgttgtc cgcgatccag
      661 gcgatgtaca cggtgtcgtt gtactcgcag cgcatgcgcc cgtattcccg cggatagatc
      721 acgaggaagc cgcaaatgtc gtcggcaaac ccttcgttgt cctggcagtt atcgcacacg
      781 tcggcatcca catagacgcc catatacacg ttgcggaggt cgatgttgcc gatatttttg
      841 atttcgtagt cgaagaggat gatgtcgtcg gcatagtcat aggaccacgc ataggaacgc
      901 tgggtgacct gaatgttcag cggccggtgg gcggtgccat cctcctcggc atcggccagg
      961 atcgtggagg tgtcggtata gagcgcaatg aaatcctgtt ccgagacggc attgacgtac
     1021 tcctccgact cggggtcgat aatcgagcgg cggacagtat tgccgaaagg ttcgtcattg
     1081 gggaaaaact gccggacatc gctccagccc tccgaaccgg tcgacacaag ggtgtcgcgg
     1141 ccgaggacgg cgccgatcca gaaggcggcc gagaacatat acatggcgcc cgtgcccttg
     1201 gggtactggc agccgccggg cggaggggtt tggcccgtga agcagtccgg agcgtcgctg
     1261 ggcacggaga attccgaacc gaaggttccg ttgttgttga cggccaggac gaggcggccg
     1321 acgttgtgca gggtcatgca gtaggccggg ctgcggctgg ccatgggccg gttgtacccg
     1381 cggccggaaa ggccgccgtc gtcacgggcc tccgcggaac cggatatcag caacaggcct
     1441 gcggcaaggg cggcagtgat agtgagcact cttcgcaaca tataccttcc catcggatta
     1501 aaccgtttta cctgacagac gcacaggacc gctcaggcgt ttaatcgcat tatgcaaagt
     1561 cacacacggg gtaaactaat gtcggtacat cggataaagc aaggaatttc ggcgaaaaaa
     1621 accgccctct ccggaggtcc gaccgcccgg ctgcccatcg gtctgaaatg tttgccaaat
     1681 ggacgtttag acgagttcgg gggatcgcag attctgccgg gccgggacgt gcaaactccg
     1741 cagggcgccg tcgctcccaa cgcctctggc cgccaatgtc cgggtccgcc aaaaaccccc
     1801 gtgcaaacac cgagtcgttg cggccgggcg ttgaaacccc gatattgcgg atcgttaacg
     1861 aaccgccggc gcgtggggga attgcggagc cgatgagtga ctgagtttcc ggtcagcggc
     1921 gtcgagacct ggtggtggtt gccatcgctg gtggctttct gcgtgtcaac gctgacttcg
     1981 accggcgggc tgtcgggggc attcctcatg ctgccctttc aggtgagcgt gctaggcttt
     2041 accgggcccg gagtcacgcc cacgaacctc ctgtacaacg tggtcggcat accgagcggc
     2101 gtctaccggt acgggcggga gaaacggatg ctctggccgc tggcttgggc gatcgtgatc
     2161 gggaccatgc ccggcatggc gatcgggacg gtcatccgcg tgaccctgct gccggacccg
     2221 tcggcgttca aagtgtttgc cggattggtg ctcgcctttg tcggcgtacg gttgctccgc
     2281 gatgtggtgc gtggggaagg cgcaaggcca tcaccgcggg gcaacggggc ggggcggttc
     2341 gaggtctcgg ggggacggct gacgaagctc actgtggagt acgatttcgg gcgccaacga
     2401 tacacggcct caactccgaa aatcctcctg ctgagtttcc tggtcggcgt ggccggcggg
     2461 gcttacggga tcggcggcgg cgcggtcatt gcgccgtttc tggtctcggc cttcggtctg
     2521 cccgtctaca ccgtggccgg ggccgcgctc ttcggcactt ttgtcagctc ggcggccggt
     2581 gtggccatgt tctggctgat cgacctctcc gggttgaccg gcagcacggc ggcccagccg
     2641 gactggctgc tgggtctggc cctggggatc ggcggggcct tcgggacgta tacgggagcg
     2701 cggctgcaga agcgggtgcc ggcgcggggc ataaaggcgc tcctgacagc gttcctgctg
     2761 tttgtcgccg ggcggtacat agtggaagct ttctgacaca acagggtcac gatatggatg
     2821 acacgattgc gaaattcgcc tccccggcgc cgattgccgt ggtgggcgca accgacgaga
     2881 cgcggaaatt cgggggctac ctgtaccgcg aattgaagcg gcaggggtac cgggtgtacc
     2941 cggtcaatgc gcggagagcg gaggtcgacg gggaccgggc gtaccggtcg ctgcgggata
     3001 tccccgacga cgtcgaggga gcggtggtcg caatccgtcc ggaccgggcg ggggaggtgg
     3061 tggcggacgc tcttgcccgc ggcattcggc gcatctggtt tcagcaagga gcggatttca
     3121 ccgaggcggc ccgcacggcc cgagaggcag gcatggagac ggttgtgggg aagtgcattc
     3181 tgatgtatgc cggaaaggcc cgcggcgtgc accggtttca cgcggtgctc gccaggctct
     3241 tcgggcggta ctgaaaatca gacggaggac gcgcgtggcg acggagattc gggaaattga
     3301 ggaccgcgac ctggacgcct tcattcgcct ccagatcgac gcctatccgg ggctgggtat
     3361 gatctacgag cgggaccggg agaaactccg gcaacggatc gcggaacggc gccaggatgt
     3421 gcgcgtgacc ctctatggaa gctaccgggg gaaccgcctg gtcgggtgca tgcgggtcca
     3481 tgactaccgg atgaatttcc gcggtgcgct ggtggcggcg ggcgggctgg gcggagtggc
     3541 ggtggatctg acggtcaagc gcgagggagt ggccggcggc atggtccggt tttacctcga
     3601 caagtatgcg gccgccggcg cgccgatggc ggtgctgtgg ccgttccggc cggactt
//
LOCUS       DAOOKS010000111         3656 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_30694_length_3656_cov_4.044710, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000111 DAOOKS010000000
VERSION     DAOOKS010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3656
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30694_length_3656_cov_4.044710"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1046)
                     /gene="tilS"
                     /locus_tag="PK112_02675"
     CDS             complement(<1..1046)
                     /gene="tilS"
                     /locus_tag="PK112_02675"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HPC10985.1"
                     /translation="MLNKVRQAIRDGHLLAPGDAVLVGLSGGPDSVALLHALWALRKE
                     LGLALTAVYINHGIRPAAAKREERFCAALCRQLAVELTIVREDIPMLAGRERKGLEET
                     ARDYRYRIYERLADERGCRRIALGHQANDQAETVLFRVLRGTGRRGLAGIPRKRGRVI
                     RPLLDVWRDEILGYLKEHRLTFCRDESNRDLRFRRNFLRHKVMPLVRKSFNPKVERAL
                     ADLADTLAAEEEYLEGVLAGVQKKVLTETPGGKFELDLRRFDGYDKWIRRRLLRYCLS
                     KISSDGTSPDKEVVERLDRAALAGRAMLSLPGGARAERIGAVLVLGRRVGAFRAELTP
                     GVPAKLERPRLNITA"
     gene            1151..2500
                     /locus_tag="PK112_02680"
     CDS             1151..2500
                     /locus_tag="PK112_02680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015379 - potassium:chloride symporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HPC10986.1"
                     /translation="MEFLGADPGRKAILYFLAAILLGAFVLSLPLSHGARPVGVIDAL
                     FTATSAVCVTGLTVVDTGTAFSPFGQVVILVLIQLGGLGIMTFATLLIAAAGAQVAFR
                     DRLGVAGSLAAGTFMRTKSLLQAVIGVTLTIELIGALVLFLLFSGSLPLGQAAWHAVF
                     HSVSAFCNAGFSTFSANLEACADRPAILLTVAALIILGGLGFAVITELLHRASGQSRR
                     LSLHTRICLSATAVLLVGGTLAVFFAEYGNTLAGFSPAGKAVNAFFQAVVCRTAGFNS
                     VSQARLTEVAVLVSMVLMFIGACPGSTAGGIKTTTITVIVTTAWDRFRGRRTPSIFHR
                     RIGEESIIRAVGVFILAVFVVAAMLALLAVLWDRPPLRAGSVHGWLAENLFEVVSAFG
                     TVGLSLGITPELNMPGKIVIILTMFIGRVGLLTLAFGLARPTATGEIVYVEEPIAIG"
     gene            2551..3201
                     /locus_tag="PK112_02685"
     CDS             2551..3201
                     /locus_tag="PK112_02685"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HPC10987.1"
                     /translation="MKEFVVIGLGNFGATVARELTRLHCRVTAVDANEARVRDLQDIV
                     HVAIVADAAERRFLEHLEVENFDAFIVSTGVDSHASILITLHLKELRARQVIVKANSA
                     DHAKILIKVGADDAIIPEEQMASRLSHSLAQSNLLDYLPLSAEHFVAELVPPDSFVGK
                     SLRDIQLRARFNIQVIATKHARTGDYIFAPGGDYVIRDHDLLIILGKEEDIDRLRQ"
     gene            complement(3208..>3656)
                     /locus_tag="PK112_02690"
     CDS             complement(3208..>3656)
                     /locus_tag="PK112_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HPC10988.1"
                     /translation="EAYGEGWALYAESLGEEMGFYRDPYAEFGRLTYEMWRAIRLVVD
                     PGLHARGWTRQQAIKFFRANSSKADHDIAVEIDRYIVWPGQAVSYKTGEMKIKALRRR
                     AEERLGERFDIRAFHDELLAHGCLPLTVLEERMAGWLAGQEAPAAR"
BASE COUNT          558 a         1398 c         1045 g          655 t
ORIGIN      
        1 gccgtgatat tcagccgcgg ccgctccagc ttcgccggta cgcccggagt caattcggcg
       61 cgaaaggcgc cgacgcgacg gcccagcacc aggactgcgc cgatgcgctc ggcgcgcgcg
      121 ccgccgggga gcgacagcat cgcccgcccg gcaagggcag cccggtcgag gcgttccacc
      181 acctctttgt ccgggctcgt tccgtcgctt gaaatttttg aaagacaata ccttaacagc
      241 cggcgacgga tccatttatc ataaccatca aaccgccgca aatcaagctc aaacttgccc
      301 cccggcgtct ctgtgagaac cttcttctgc accccggcca gcactccctc gaggtactct
      361 tcctccgccg ccagcgtatc ggccagatcc gcaagggccc gctctacctt cgggttgaag
      421 ctcttccgga caagaggcat gaccttgtgc cgcaaaaagt tgcgccggaa ccggaggtcc
      481 cggttgctct cgtcgcggca gaaagtcagg cgatgctcct tcaggtagcc caggatctca
      541 tcccgccaca catcgagaag cggccggatc acccgcccgc gtttccgcgg gatccccgcc
      601 agcccccgcc gccccgtccc gcgcaggacg cggaacagaa ccgtctccgc ctgatcattc
      661 gcctggtggc cgagtgcgat gcgccggcag ccccgctcgt ccgcgagccg ctcatagatc
      721 cggtaccgat agtcccgggc cgtctcctcc agacccttcc gctcccgtcc ggccagcatc
      781 gggatatcct cgcgcacgat cgtcagttcc accgcgagct gccggcacag ggcggcgcag
      841 aagcgctcct cccttttcgc agccgccggt cggatcccgt gattgatgta cacggcggtc
      901 agggccaggc cgagctcctt ccggagcgcc cacagggcgt gcagcagcgc caccgaatcc
      961 ggtccgcccg aaagcccgac caggaccgcg tcgccgggcg ccaggagatg gccgtcccga
     1021 atcgcctgtc gcaccttgtt cagcatcacc tcagaagata caggccggcg ggcaaaggtc
     1081 gagcgaaatc ttagtgcgcc attcagacct tgacaggtcc atcggcgggc ggccatattg
     1141 gcccccagcg atggagttcc tcggcgctga tcccggccgg aaagcaatcc tctacttttt
     1201 ggccgccatt ttgctcggcg cgttcgtgct gagcctgccg ctcagtcacg gcgcccggcc
     1261 ggtcggggtc atcgacgccc tcttcactgc cacctccgcc gtgtgcgtta ccggcctcac
     1321 cgtggtcgac accggcacgg cgttctcccc cttcggccag gtcgtaatcc tcgttctgat
     1381 tcagctcggc gggctcggta tcatgacgtt cgcgactctc ctgatcgccg ccgccggggc
     1441 acaggtcgct ttccgcgacc ggctcggcgt ggccgggtcg ctcgccgccg ggaccttcat
     1501 gcgcaccaag tcgctcctcc aggccgtcat cggcgtcacc ctcaccatcg aactgatcgg
     1561 cgccctggtc ctcttcctcc tcttcagcgg gtcgctgccg ctcgggcagg ccgcctggca
     1621 cgccgtcttt cactcggtca gcgccttctg caacgccggg ttctcgactt tctccgccaa
     1681 ccttgaggcc tgcgccgacc ggccggccat cctgctgacc gtggcggccc tgattatcct
     1741 cggcgggctc gggttcgccg tcattaccga gctcctccac cgcgcctccg gccagtcccg
     1801 tcgcctctcg ctgcacacgc gcatctgcct gagcgcgacc gcggtcctgc tcgtcggcgg
     1861 cacgctggcg gtcttctttg ccgagtacgg caacacgctg gcgggtttca gcccggccgg
     1921 caaagccgtc aacgccttct tccaagcggt cgtctgccgc accgccggct tcaactccgt
     1981 ctcccaggcc cggctcaccg aggttgccgt gctggtctcc atggtgctca tgttcatcgg
     2041 cgcctgcccg ggctcgaccg ccggcggcat caagaccacc accattaccg tcattgtcac
     2101 caccgcctgg gaccgcttcc ggggacgccg cactccgtcg atcttccacc gccgcatcgg
     2161 cgaggagtcc atcatccgcg cagtcggcgt gttcatcctc gcggtgttcg tcgtcgccgc
     2221 catgctcgcc ctgctcgccg tcctctggga ccggcccccc ctgcgcgccg gctccgtcca
     2281 cggctggctg gccgaaaacc tcttcgaggt cgtctccgcc ttcggcacgg tcggcctctc
     2341 cctcggcatc actcccgaat tgaatatgcc gggaaaaatc gttatcatcc tgaccatgtt
     2401 catcggccgc gtcggactgc tgacgctggc cttcggcctg gcgcggccga ccgcgaccgg
     2461 ggagatcgtt tatgtcgagg aaccgatcgc catcggctga gcgcggccgc cgtcccgcag
     2521 agggaacaac agtttaaact ggttgggctt atgaaagaat tcgtcgtcat cggcctgggc
     2581 aatttcggcg ccaccgtcgc ccgcgagctc acccgcctcc actgccgcgt gaccgcggtc
     2641 gatgccaacg aggcccgggt gcgcgatctg caggacatcg tccacgtcgc cattgtggcc
     2701 gacgccgccg aacgccgctt cctggaacac ctcgaggtcg agaacttcga cgccttcatc
     2761 gtctcgaccg gcgtcgattc ccacgcctcc atcctcatca ccctccacct caaagagctg
     2821 cgcgcccgcc aggtgatcgt caaggccaac tccgccgacc acgccaagat tctgatcaag
     2881 gtcggcgccg acgacgccat catccccgag gagcagatgg cctcgcgcct gtcccactcc
     2941 ctggcccagt ccaacctgct ggactacctg cccctctcgg ccgagcactt cgtcgccgaa
     3001 ctcgtccccc cggacagttt cgtgggcaag tcgctcaggg acatccagct ccgcgcccgc
     3061 ttcaacatcc aggtcatcgc caccaaacac gcccgcaccg gcgactacat cttcgccccc
     3121 ggcggcgact acgtcatccg cgatcacgac ctgctcatca tcctcggcaa agaggaagat
     3181 atcgaccgcc tccgccagtg accggtctca ccgggccgcc ggcgcctcct gcccggccag
     3241 ccaccccgcc atccgctcct ccaggacggt caggggaagg cagccgtggg cgagcagttc
     3301 atcgtggaag gcgcggatgt cgaaacgctc ccccaggcgc tcttcggccc ggcgccggag
     3361 ggccttgatt ttcatctcgc ccgtcttgta cgacactgcc tggccgggcc agacgatgta
     3421 gcggtcgatc tcgaccgcga tatcgtggtc ggccttgctg gaattcgccc ggaagaactt
     3481 gatcgcctgc tgccgggtcc agcccctggc gtggaggccc ggatcgacca ccaggcggat
     3541 cgcccgccac atctcgtagg tcagccggcc gaattccgca taggggtcgc ggtagaatcc
     3601 catctcctcg ccgaggctct cggcgtagag cgcccagcct tcgccgtatg cctcca
//
LOCUS       DAOOKS010000112         3646 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_30793_length_3646_cov_2.908661, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000112 DAOOKS010000000
VERSION     DAOOKS010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3646
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30793_length_3646_cov_2.908661"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="PK112_02695"
     CDS             complement(<1..405)
                     /locus_tag="PK112_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020469565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HPC10989.1"
                     /translation="MNLTLYVWRQKDGRSKGRMERYEAKNVNEHMSFLEMLDVVNEEL
                     IEKGEEPIAFDSDCREGICGTCGMMINGIAHGPQRGTTVCQLHMRFFKDGDQIYIEPF
                     RSRAFPIVKDLVIDRSALDRVIAAGGFVSVNTG"
     gene            complement(405..2318)
                     /locus_tag="PK112_02700"
                     /pseudo
     CDS             complement(405..2318)
                     /locus_tag="PK112_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016753085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
     gene            complement(2322..3056)
                     /locus_tag="PK112_02705"
     CDS             complement(2322..3056)
                     /locus_tag="PK112_02705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HPC10990.1"
                     /translation="MATNQISRVTDTKVIPEPPTMTSIVRKFWMAVSGVIILTFVVGH
                     MLGNLQVFLGPDQINAYAQKLRAVPALLWAVRVFFLIWALVHVVDGAVLWLRNRQSRP
                     QTYVKQRFQEATLASRTMIWTGLAIFFFIVYHLLHYTLFVTNPEYAGLTLPDGHHNVY
                     AMLVQGFQNPLISVVYILAIFFLAVHLTHSISSFFQTFGWTRTELQPIFRGIAWTAAA
                     IIFIGYVSIPIGVLTGIIAPMAERGF"
     gene            3291..>3646
                     /locus_tag="PK112_02710"
     CDS             3291..>3646
                     /locus_tag="PK112_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10991.1"
                     /translation="MVFRILMTAVAAVLLCAGLGSAGTVGVKLSGDGVLNDTTVKAGL
                     PVSVDFYIENDSLFTGFAFGFALTSPDSSVKRVIHVADSGNGLNPAGDIKGFNGWQDQ
                     SVWNFGGVYAVESDWNG"
BASE COUNT          630 a         1222 c         1105 g          689 t
ORIGIN      
        1 gcccgtgttg accgacacga agccgccggc ggcgatcacc cggtcgagcg ccgagcggtc
       61 gatcacgagg tccttgacga tcgggaaggc gcgggagcgg aagggctcga tgtagatctg
      121 atcgccgtcc ttgaagaacc gcatgtggag ctggcacacg gtcgtgcccc gctgcggtcc
      181 gtgggcgata ccgttgatca tcatcccgca cgtcccgcag atcccttccc ggcagtcgct
      241 gtcgaacgcg atcggctcct cgcccttctc gatcagttcc tcgttgacca cgtcgagcat
      301 ctcgaggaac gacatgtgct cgttgacgtt tttcgcctcg tagcgttcca tccgcccttt
      361 cgagcggccg tccttctggc gccatacgta aagtgtgagg ttcattactt gtaactcctt
      421 gtcgccagct cgacgaactc aaacgcgagc ggttccacgt gccgcgccgg tttgacgccg
      481 acgcccttgt actcccaggc tgcgacgtgg gcgaagtccg cgtcattgcg gagcgcctcg
      541 ccgtcgggcg tctgatgctc ctcgcggaaa tgcgccccgc acgattcttc gcgggtcagc
      601 gcatcccagc acaggacttc ggcgaactcc agaaaatcgg ccacccggcc ggctttctcc
      661 agcgcctggt tgagctcttc gccgtcccct ggcaccgaca ggtttcgcca gaattcctcg
      721 cggatctcgg ggagcttggc cagcgcttcg cgcaggccgg tcgcgttgcg gcccatgccg
      781 cacttctccc acatcagctt ccccatctcc cggtggaacg cgtcgggcga gcgcttcccc
      841 ttgatggcca gcagcttcct gatccggtcg gccgcctcct gttccaccgc ccggaactcg
      901 ggccggtcga tcccgatatt cttcgccccg gtccgggcga agtagtggga aatggtcgcg
      961 ggcagaatga aatacccgtc ggccagcccc tgcatgagcg cgctcgcgcc cagccggttg
     1021 gccccgtgat ccgagaagtt cgcctccccg atcacgaaca gccccgggat cgtgctctcc
     1081 aggttgtagt ccacccacag cccgcccatg gtgtagtggg aggccgggta gatgcgcatc
     1141 ggcgactcgt acggattctc cccggtgatc ttcgcgtaca tgtcgaacag gtttccgtac
     1201 cggtctgcaa tcacgtcttt gccgagccgc ttgatggcat cgcggaaatc aaggtacacg
     1261 ccccggccgc ccggtccgac cccccgcccc tcgtcgcaca cttccttggc cgatcgcgag
     1321 gaaatatcgc gtggcgcgag attgccgtaa ctcgggtact tccgctccag gaaataatcc
     1381 cggtccgcct ccgggatctg gttcggcccg cggctgtcgc cggcctgccg ggggacccag
     1441 atccgcccgt cattgcgcag cgactccgac atgagcgtca gcttcgacta gtattctccc
     1501 gacaccggga tgcaggtcgg gtgaatctgc gtgaaacacg ggttggcgaa cgcggccccg
     1561 cgcttgtacg cgcggtaggt cgccgtcacg ttgcacccct tggcgttggt cgagaggtgg
     1621 aagacgttcg cgtagccgcc cgtggccagc accaccgcgt cggccgagtg cgaatagatg
     1681 ccgccgccga tcatgtcccg caccacgatc cccttcgcgt ggccgtcgac cagcacgagg
     1741 tcgagcatct ccgtccgcgg gtacagcttc acctgccccc ggccgacctg ccgcatcatc
     1801 gcctggtagg ccccgatgag cagctgctgc cccgtctggc cgcgcgcgta gaatgtccgc
     1861 gacacctgcg ccccgccgaa cgaccggttg tccaggtagc cgccgtagtc gcgggcgaac
     1921 ggcactccct gggccacgca ctggtcgatg atgttttcgc tcacctgggc cagccggtag
     1981 acgttcgcct cgcgcgagcg gaaatccccg cccttgatgg tgtcgtagaa cagccggtac
     2041 accgaatcgc tgtcgttctg gtagttcttc gaggcgttga tgcctccctg ggccgccacg
     2101 ctgtgcgccc ggcgggcgct gtcctggtag cagaatacgg ccacgttgta ccccagttcg
     2161 cccagggtgg ccgccgcgct ggctccggcc agccccgacc cgacgatgat cacgctgaac
     2221 ttccgcttat ttgccgggtt gaccagcttg ctctcgaatt ggtgcttgtc ccacttctcg
     2281 gccagcggcc ccgatggtac tttgctgtca agcttcatgc ctcaaaaccc cctttccgcc
     2341 atcggcgcga tgatccccgt cagcacgccg atcgggatgc tgacatagcc gatgaagatg
     2401 atggccgcgg ccgtccaggc gatcccgcgg aaaatcggct gcagctccgt gcgcgtccag
     2461 ccgaacgtct ggaagaaact cgaaatggag tgggtgaggt gcacggcgag gaagaagatc
     2521 gcgagaatgt acacaaccga gatgagcggg ttctggaacc cctgcacgag catcgcatag
     2581 acattgtgat ggccgtccgg gagcgtcaga ccggcatact ccgggttcgt cacaaacagc
     2641 gtgtagtgga gaaggtggta gacgatgaag aagaagatcg ccagccccgt ccagatcatc
     2701 gtccgcgagg ccagcgtcgc ctcctggaac cgctgtttga cataggtctg cggccgcgac
     2761 tggcggttgc ggagccacag cacggcgccg tcgaccacgt gcactagcgc ccagatgaga
     2821 aagaacaccc ggaccgccca caagagcgcc ggaaccgccc ggagcttctg cgcgtaggcg
     2881 ttgatctggt cgggacccag gaacacctgg aggttcccca gcatgtgccc gaccacgaaa
     2941 gtaaggataa taacccccga gacggccatc cagaacttgc gcacgatcga ggtcatggtc
     3001 ggcggctcgg ggatgacttt tgtgtcggtg acacgactga tctgatttgt cgccatcggc
     3061 cctcctttcc tgtcgcgaag atacagccgt ccgccccgga cggtcaaaca aaaagacaat
     3121 cggaccgcgc gcgggagatt ggccgcccgc gcggacgatt ttcgcccggc cgggtataag
     3181 acatacagcc gtgagaatcc gtttgacaag ccccggcggc tgacctatgt tgagaatcat
     3241 gcgaagagat cacaactgtt tcagatacag tagcttttga ggagattcca atggtgttcc
     3301 gaattctcat gaccgcggtt gccgccgtgc tgctctgcgc cgggctgggc tcggccggca
     3361 ctgtcggagt caagctctcg ggcgacggcg tcctcaatga cacgaccgtc aaagccggtc
     3421 tcccggtctc cgtcgacttc tacatcgaga acgactcgct cttcaccggt ttcgctttcg
     3481 gcttcgccct gacctcgccg gattcctccg tcaaacgggt gatccacgtc gccgacagcg
     3541 gcaacggcct caaccccgcc ggcgatatca aggggttcaa cggctggcag gatcagtcgg
     3601 tctggaattt cggcggcgtc tatgcggtcg agtccgactg gaacgg
//
LOCUS       DAOOKS010000113         3639 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_30880_length_3639_cov_3.198103, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000113 DAOOKS010000000
VERSION     DAOOKS010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3639
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30880_length_3639_cov_3.198103"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /locus_tag="PK112_02715"
     CDS             <1..883
                     /locus_tag="PK112_02715"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HPC10992.1"
                     /translation="DVGPVVDVPAPDVMTNGQHMLWMLDEFEHIHGGKYPGFITGKPV
                     GMGGSLGRTEATGFGVVFTLREALKQLHIQPDKTAASVQGFGNVAQYAIKLYQQLGGT
                     VIAVSSWDQADQTSYTFRRTSGIDLQQLLGITDRFGGIDKGKAAGLGYEVLPGEAWLE
                     QEVDVLIPAALENQISGENVEKISRRVKVIAEGANGPTTPEADKVISARNIFVIPDFL
                     ANAGGVTCSYFEQVQSNMNYYWPKEEVLQKLDQKMTAAFLAVSELASRKKLYMRDAAY
                     VIAVGRVAQACRDRGWV"
     gene            1058..>3639
                     /locus_tag="PK112_02720"
     CDS             1058..>3639
                     /locus_tag="PK112_02720"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HPC10993.1"
                     /translation="MTDEQKPIGDYWVLDRLIGSLQERAKELNCIYRVGAILTNPDEP
                     IGRVCPLLIEAIPTGWQYPKLCQVKIVIEDNIFATPNFIETPWLQSADIAVQDIVIGS
                     ITVSYREETPSAAEKLFLADESRLLKTIAERLGYFICHQKRRQITEEWQTVRPEVSVG
                     RPEWQVVLKLLRDTDRELYQTIARKMLNHLCWSGIAEAEKLLRSFTSNESLEDEENPE
                     HWNRPHQRKSLVMSAAFSSAAFKIAADHLDAGEILNLIQKWIQEDKLGFLVQAVNRNL
                     TLSEVADAIRRYQHLTQDEPVTLSSSMRGIQVSLIRRFLSDQQQYINVAKNYVDVADF
                     CKLIQNLIYTSESYGRLGGKSAGFFLAKQILLKKAADNELLANVQIPKTWYITSDVIL
                     YYMHYNNMDDVVEQKYKDIGQIRREYPHVVQSFKNARFPADIIKGLSMALDDLGDRPL
                     IVRSSSLLEDRVGAAFSGKYKSLFLANRGSKQQRLDALTDAIAEVYASTFGPDPIEYR
                     AERGLIDFAEEMGIIIQEVVGRRIGKYFLPSFAGVAFSNNEFRWSPRIRRTDGLIRLV
                     PGLGTRAVDRLSNDYPILIAPGQPTLRTNVTPDEIARYSPKYIDVINLETEDFETIPL
                     LDFLREVNFEVPGIQHMLSLSMDGRLVAPGLTADISSGAPVVTFDGLVSRTRFLKRLR
                     AIMQTLEVTLGEPVDIEFACDGDDFYLLQCRAQSYSAESAPAPIPKDIPMQQIVFSAN
                     RYVSNGRVPDVTHVVYVDPRKYAEIEDRATLVAVGRAVSRLNKLLPKRKFILIGPGRW
                     GSRGDIKLGVNVTYSDINNTSMLIEVAIRHGNYVPELSFGTHFFQDLVEANIRYLPLF
                     PGDD"
BASE COUNT          698 a         1144 c         1185 g          612 t
ORIGIN      
        1 agatgtcggg ccagtagtcg acgtgcccgc gccggacgtc atgaccaacg gccagcacat
       61 gctgtggatg ctcgatgaat tcgagcacat ccacggcggc aagtatccgg ggttcatcac
      121 cggcaagccg gtggggatgg gcggctcgct cggccgcacc gaggccaccg gcttcggcgt
      181 cgtgttcacg ctgcgcgagg cgctcaagca gctccacatt cagccggaca agacggcggc
      241 cagcgtgcag ggattcggca acgtggcgca atacgccatc aagctctacc agcagctcgg
      301 cgggacggtg attgcggttt ccagctggga ccaggcggac cagacctcgt acacgttccg
      361 gcgcacgagc ggcatcgatc tccagcagct cctgggcatc accgaccgct tcggcggcat
      421 cgacaagggt aaagccgccg ggctcggcta cgaggtgctg ccgggcgagg cctggctgga
      481 gcaggaggtc gacgtgctca tcccggccgc cctggaaaac cagatctcgg gcgagaacgt
      541 ggagaagatc tcgcggcggg tgaaggtgat tgcggaaggg gcgaacggtc cgacgacacc
      601 ggaggccgac aaggtcatct cggcccgcaa catcttcgtc atccccgact tcctggcgaa
      661 tgcgggcggc gtgacctgca gctacttcga gcaggtgcag tcgaacatga actactactg
      721 gccgaaggag gaagtgctgc agaagctcga ccagaagatg acggcggcct tcctggcggt
      781 cagcgagctg gcgagccgca agaagctgta catgcgcgac gcggcctacg tgatcgccgt
      841 gggccgggtg gcgcaggcct gccgggatcg cggctgggtc tgagatttag tgcggtgaac
      901 gcaagtcaac ggccgtgcgc agcccgcgcg cggccgtttc ctgttgcatt gggcgcgttc
      961 ccgcgcgata ttgtcgcgag gggcaggccg atgcggggcg caggccggcg ccgccgcgcc
     1021 gcaggccgcc ccgccgacgc gaaggaatca cagctctatg accgatgaac agaaaccgat
     1081 aggagactac tgggtgttgg atcgcctgat cggctctctc caggagcggg ccaaggagct
     1141 caactgcatc taccgggtgg gcgcgattct caccaatccg gacgagccga tcgggcgcgt
     1201 ctgcccgctc ctcatcgaag ccattccgac cggctggcag taccccaagc tgtgccaggt
     1261 caagatcgtc attgaggaca acatcttcgc caccccgaac ttcatcgaga cgccctggct
     1321 gcagtcggcc gatatcgccg ttcaggatat cgtcatcggg tcgatcacgg tatcctaccg
     1381 cgaggagacg ccgagcgcgg ccgagaagct gtttctcgcc gatgagtcgc ggctgctcaa
     1441 gacgatcgcc gagcgtctcg gatatttcat ctgccaccag aagaggcggc agatcacgga
     1501 ggagtggcag acagtccgcc ccgaggtctc ggtcgggcgc cccgagtggc aggtggtgct
     1561 gaaactcctg cgggacacgg atcgggagct ctaccagacg atcgcgcgga agatgctcaa
     1621 ccacctgtgc tggagcggca tcgccgaggc cgagaaactc ctccgctcgt tcacctcgaa
     1681 cgagtcgctg gaggacgagg aaaacccgga gcactggaac cgaccgcacc agcgcaaatc
     1741 gctggtgatg tcggcggctt tcagttcggc ggctttcaaa atcgcggccg accacctcga
     1801 cgccggcgag atcctcaacc tcatccagaa gtggatccag gaggacaagc tcggcttcct
     1861 cgtccaggcg gtcaaccgga acctgacgct gtcggaggtg gccgacgcca ttcggcgcta
     1921 ccagcacctc acccaggacg agccggtgac gctgtcgtcg tcgatgcggg ggatccaggt
     1981 gtcgctgatc cggcggttcc tgtctgacca gcagcagtat atcaacgtgg cgaagaacta
     2041 tgtcgatgtc gccgacttct gcaagctcat ccagaacctg atctacacat ccgagagtta
     2101 cgggcggctg gggggcaaga gcgccggctt tttcctggcc aagcagattt tgctcaagaa
     2161 agccgcggac aacgagctgc tggccaacgt gcagatcccc aagacctggt acatcacctc
     2221 cgacgtcatc ctctactaca tgcactacaa caacatggac gacgtggtgg agcagaagta
     2281 caaggacatc gggcagatcc gccgcgagta cccgcacgtc gtccagtcgt tcaaaaacgc
     2341 ccgcttcccc gccgacatca tcaagggact ctcgatggcg ctcgacgacc tgggggaccg
     2401 cccgctcatc gtgcgcagct cgagccttct ggaagaccgg gtcggggcgg cgttctccgg
     2461 caagtacaag agcctctttc tggccaatcg cggctccaag cagcagcgcc tggacgcgct
     2521 gacggatgcg atcgccgagg tctatgcgag cacgttcggc ccggacccca tagaataccg
     2581 cgccgagcgc gggctgattg atttcgccga ggagatgggg atcattatcc aggaggtggt
     2641 ggggcggcgg atcgggaagt atttccttcc ctcgtttgcg ggggtggctt tcagcaacaa
     2701 cgaattccgc tggtcgccgc ggattcggcg gacggacggt ctcatccggc tggttcccgg
     2761 cctgggcacc cgcgccgtcg accggctgag caacgactac ccgatcctga tcgcgccggg
     2821 gcagccgacg ctgcggacga acgtgacgcc ggacgagatc gcgcggtact cgccgaagta
     2881 tatcgacgtg atcaatctgg agacggagga ttttgagacg attccgctgc tggactttct
     2941 ccgggaggtg aatttcgaag ttccggggat ccagcacatg ctgtcgctca gcatggatgg
     3001 gcggctggtg gcgccgggcc tgacggccga tatttccagc ggcgcgccgg tggtgacgtt
     3061 cgacggcctg gtgtcgcgga cgcgcttcct caagcgtctg cgggcgatca tgcagacgct
     3121 cgaggtcacc ctgggagagc cggtggatat cgagttcgcg tgcgacgggg atgatttcta
     3181 cctgctccag tgccgggccc agagctactc agcggaaagc gccccggccc ccattcccaa
     3241 ggatatcccg atgcagcaga ttgtcttctc ggccaaccgc tacgtctcca acggccgggt
     3301 tccggatgtg acgcacgtcg tctacgtgga cccgcggaag tacgccgaga tcgaggaccg
     3361 ggccacgctg gtggcggtgg ggcgggcggt cagccgcctg aacaagctgc tgcccaagcg
     3421 caaattcatc ctcatcggac cgggacgttg ggggagccgc ggcgacatca aactcggcgt
     3481 caacgtcacc tactccgaca tcaacaacac ctccatgctc atcgaggtgg cgatccgcca
     3541 cggcaactac gtgcccgaac tctcgttcgg cacgcacttc ttccaggacc tggtggaggc
     3601 caacatccgg tacctgccgc ttttcccggg ggacgatca
//
LOCUS       DAOOKS010000114         3598 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_31346_length_3598_cov_4.193057, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000114 DAOOKS010000000
VERSION     DAOOKS010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31346_length_3598_cov_4.193057"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            170..1258
                     /locus_tag="PK112_02725"
     CDS             170..1258
                     /locus_tag="PK112_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006207822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional activator/DNA
                     repair protein Ada/methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HPC10994.1"
                     /translation="MSHLPPPAIMYRALRDRDATFEGVFYVGVRTTGIFCRPTCPARK
                     PKEENVQFFAFPREALQAGYRPCARCAPLEVERPAPALVRALRNLVDESPGGKISGAL
                     LRARGIDPSTARRQFQRYYGMTFHAYQRARRLGLALHEIRRGESVIGAQIDHGYESAS
                     GFFEAFRNAFGAPPSRLETVACLFAQAIETPLGPMVAVADDSGLHLLDFVDRGGWQQT
                     VGKLRERSATAVVPGPHPHLAAVAEQLKSYFEGRSLDFSVPLVPDGTPFELAVWSLLQ
                     TIPPGAAWSYAQMAARLGRPAAVRAVGRANSRNRLALVIPCHRVIRADGTLCGYGGGI
                     WRKQWLLAHERRVLSRRETSPVTPGPTG"
     gene            complement(1236..2678)
                     /locus_tag="PK112_02730"
     CDS             complement(1236..2678)
                     /locus_tag="PK112_02730"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPC10995.1"
                     /translation="MTTAVDTGKTRVAETERDEKLVNAFFVLYRNARIMEPANKAFVK
                     QCAHFLELLQPGLKADAEVAIKAVRGRYFTNERFVRFSDPSGAAAAVTADWEALGIGG
                     VRFLPEVTEDHLRGFFSFMAALTPRGRSLAELQEELDRHIPATVFLLALPDENEDDED
                     EAERRRQLRLQARTTFFRAVSAVSELLARTAVERDVNLAKTRRVVHALIDHLSKDKDS
                     LVELSAIKDFDDYTYAHSVNVSVYSLTLGIRLGLDRPRLSQLGFAALFHDAGKVRLDR
                     DLINKPDSFDENDWIQMQMHPALGAKTILRSMKLDQHTARAARGAFEHHINADYTGYP
                     LLHDIRPLNLYSQIITIADTFDALTSDRVYFRKAMAPDQVIRKMRFQMTAKFNPILIA
                     LFNEVMGIYPAGSMALLSTDEIAVIMAANAELYDRPYVQIVGNREGLLPEPEWVDLAR
                     ADQINRRIVRLIDPERYGLKVRDFILSGPE"
     gene            complement(2675..>3598)
                     /locus_tag="PK112_02735"
     CDS             complement(2675..>3598)
                     /locus_tag="PK112_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10996.1"
                     /translation="AQGQVAHAAFLLRSLRNLGERIRGAKPRWADRLEGIYMTAGSRE
                     RLRLLAKALNEHTEITESDLRRYLDLFDWQALSALTDLLGELEHRPHRETLCRYLVDH
                     GRGKEEILAKGVYDKRWYVVRNSVMILGHIGDDRALQHLKQAVAHEDRRVRIELVEAV
                     RGKEHAGALEILGRLVFDPDEEVHREAMAALMGSSGAGAFEALAGAVTAESFGQLDPQ
                     DQIALLVAYSRRGKERAVPHLARLIARFNPTRNPIRTFFRAAAFDALEQNPSEKAGAL
                     LQRLTGSWRPDIRRRATRCLSRRKAPTGGEA"
BASE COUNT          531 a         1242 c         1194 g          631 t
ORIGIN      
        1 ggaccgtgct cggcacggag cggatcgtcg acatgcccgt gggcgagcaa ttgccgcgca
       61 tttgctgacg tctctttccg gaattccgtg ccccgttttc ggatgcgcga acggcccgcc
      121 cccgttacct tgggacaaaa agcttaaccg gagatacgaa ccaccgacca tgagccacct
      181 gccgcctccc gccatcatgt accgggcgct tcgcgaccgt gacgccacct tcgagggcgt
      241 cttctacgtc ggcgtccgca ccaccggcat cttctgccgg cccacctgcc cggcccgcaa
      301 acccaaagag gagaacgtcc agttcttcgc cttcccgcgg gaggcgctcc aggccgggta
      361 tcgcccctgc gcccggtgcg ctccgctcga ggtcgagcgg cccgccccgg cgctggtccg
      421 cgccctgcgc aacctggtcg atgagtcccc cggcgggaag atttccggcg ccctactccg
      481 cgcgcgcggg atcgatcctt cgaccgcccg gcggcagttc cagcgctact acggcatgac
      541 cttccacgcc taccagcgcg cccggcgcct gggcctggcg ctccacgaaa tccgccgggg
      601 ggaatcggtc atcggggccc agatcgacca cgggtacgag tccgccagcg gcttcttcga
      661 ggccttccgg aacgccttcg gcgcaccgcc gagccgcctc gagacggtgg cttgcctgtt
      721 cgcgcaggcg atcgagacgc cgctcggtcc gatggtcgcg gtggccgacg actccgggct
      781 gcatctgctc gattttgtcg accgcggcgg gtggcagcaa accgtcggga agctccggga
      841 acggtcagcg accgcggtcg tccccggtcc ccatccgcac ctcgccgcgg tggcggagca
      901 actgaagagt tatttcgagg ggcgttcgct ggacttctcg gtcccgctcg ttccggacgg
      961 cactcccttc gaactggcgg tctggtcgct tctgcagact atcccgccgg gggccgcctg
     1021 gtcatatgcg cagatggccg cgcggctcgg ccgtccggcc gccgtgcgcg cagtcgggcg
     1081 ggccaacagc cgcaatcgcc tggccctcgt gatcccctgc catcgggtga ttcgtgcgga
     1141 cggaaccctg tgcgggtacg gcggcgggat atggcgcaag cagtggctgc tggcccatga
     1201 gcggcgggtg ctgtcgcgcc gggaaacgag tccggttact ccgggcccga caggatgaag
     1261 tcgcgaacct tcaggccgta gcgctccggg tcgatcagcc ggacgatgcg gcggttgatc
     1321 tgatcggcgc gggccagatc gacccactcc ggttccggca ggaggccctc ccggttgccc
     1381 acgatctgga catagggccg gtcgtacagc tccgcattag cggccatgat caccgcgatc
     1441 tcatccgtgc tcaacagcgc catgctcccg gccgggtaga tgcccatcac ctcgttgaac
     1501 agggcgatca ggatcggatt gaatttggcc gtcatctgga accgcatctt ccggatcact
     1561 tgatccggcg ccatcgcttt ccggaaatac acccggtcgg aggtgagggc atcgaacgtg
     1621 tcggcgatcg tgataatctg cgagtacagg ttcagcggcc ggatgtcgtg gagcaggggg
     1681 tagccggtgt aatcggcgtt gatgtggtgt tcgaacgccc cgcgcgccgc ccgcgccgtg
     1741 tgctggtcga gcttcatgct gcgcaggatc gtcttggccc ccagcgccgg gtgcatctgc
     1801 atctggatcc agtcgttctc atcgaacgag tccggcttgt tgatcaggtc gcggtccagc
     1861 cgcaccttcc ccgcgtcgtg gaagagcgcg gcaaagccga gctgtgacag gcgcggacgg
     1921 tcgaggccca ggcggatccc cagagtcagc gagtacaccg acacgttgac cgagtgggcg
     1981 taggtgtagt cgtcgaaatc cttgatggcc gacagctcca ccagcgaatc cttgtccttc
     2041 gacaggtggt cgatgagcgc gtgcacgacc ctccgggtct tggccagatt gacatcgcgc
     2101 tccacggccg tgcgggcgag cagttccgag actgcggaga cggcgcggaa aaacgtcgtc
     2161 cgcgcctgga gccggagctg gcggcgccgc tccgcttcgt cttcgtcgtc ttcgttttcg
     2221 tccggcagcg cgaggaggaa gacggtggcg gggatgtggc ggtcgagttc ctcctgcagt
     2281 tccgcgagac tccgcccgcg gggcgtcagc gcggccatga aactgaaaaa cccgcgcaga
     2341 tgatcctcgg tgacctccgg caggaaacgg accccgccga ttccgagggc ctcccagtcg
     2401 gcggtgacgg cggcggccgc gccgctgggg tcggagaagc ggacgaagcg ctcgttggtg
     2461 aaatagcggc cccgcaccgc cttgatcgcc acctcggcat cggccttcag ccccggctgg
     2521 agcagctcga gaaaatgggc gcactgcttc acgaacgcct tgttggccgg ctccatgatt
     2581 cgggcgttcc ggtacagcac gaagaaggcg ttgacgagct tttcgtcccg ttccgtttcc
     2641 gccacccggg ttttgccggt atcgactgcg gtcgtcatgc ctcacctccg gtcggcgctt
     2701 tgcggcggct cagacagcgg gtcgcgcgcc ggcggatatc cgggcgccac gagccggtca
     2761 ggcgttgcag gagcgcgccc gctttctcgc tcgggttctg ctcgagggca tcgaatgcgg
     2821 ccgctcggaa aaacgtccgg atcggattcc gcgtcgggtt gaagcgggcg atcagccggg
     2881 ccaggtgcgg caccgcccgc tctttccccc ggcgcgagta cgccaccagc agcgcgatct
     2941 ggtcctgggg atcgagttgg ccgaacgatt cggccgtgac cgctccggcg agcgcctcga
     3001 acgcgcccgc cccggaactc cccatcagcg cggccatcgc ctcccggtga acttcttcgt
     3061 ccgggtcgaa taccagccgg ccgagaatct cgagggctcc ggcgtgctct ttcccccgca
     3121 ccgcctccac cagctcgatc cgcacccgcc ggtcctcgtg ggcgaccgcc tgtttcaggt
     3181 gctggagcgc ccggtcgtcg ccgatgtggc cgaggatcat caccgaattc cgcaccacgt
     3241 accaccgttt gtcgtacaca cccttggcga ggatttcctc cttgccgcgg ccgtgatcca
     3301 ccaggtaccg gcagagcgtc tcgcggtggg ggcggtgctc cagttcgccg agtagatcgg
     3361 tcagcgcgct cagggcctgc cagtcgaaca ggtcgaggta ccgccggagg tcgctctcgg
     3421 tgatctcggt gtgctcgttc agcgctttgg ccaggaggcg caggcgctcc cggctcccgg
     3481 cggtcatgta gatcccttcc agccggtcgg cccagcgcgg cttggctccc cggatgcgct
     3541 ctccgagatt cctcagggac cgcagcagga aggccgcatg cgccacctgg ccctgggc
//
LOCUS       DAOOKS010000115         3595 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_31390_length_3595_cov_2.843220, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000115 DAOOKS010000000
VERSION     DAOOKS010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3595
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31390_length_3595_cov_2.843220"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1625)
                     /locus_tag="PK112_02740"
     CDS             complement(<1..1625)
                     /locus_tag="PK112_02740"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HPC10997.1"
                     /translation="MKMARAWVWLWLAAGAAASPAREAGFEGPRPVNAAAVERAARQP
                     AVSLDSLERMLQTEGYMDAAVSQEEGRVRVRAGDRARLRVATVISGADTTALRLEAWF
                     REAEVTRLIDSLLDRQRECGYYYASITISGIERSGDDIMLYMSLVPGPVMRLGEVHYL
                     GLRRTKPATAVRYLTANAGDSLTDRYLVRLEREAAEIPWLRFLPPATVRPLPGFTSAD
                     VELQFVEERPLRLDVGGGYIPDTSEATVVWHVDARLNNLFGGGRRARVLSERRDKDRR
                     LLQVSYLQPLFFAGRAEGRIEAATRDYRTLFYEFALRAGYAVRLAQGVTAGVDLEYRG
                     VEPAEGGRSYDAYAAEFSARWDRVDEPLNPANGFEARTTISYGHRKYARGAAGEGERT
                     QYGETRTVFEARVYRRIAGPLAACAGLGYRGFETSEDFPPPAELNLIGGPGTLRGYRN
                     EQFAALRAVLATIEPRIRFGAGYAFGFYDGAFLSNRVSAAGGGRTEESYRSGFGGGVA
                     LTDGVRAVTVALGWNPEVPFREPRLSIEVRSGF"
     gene            complement(1625..>3595)
                     /locus_tag="PK112_02745"
     CDS             complement(1625..>3595)
                     /locus_tag="PK112_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC10998.1"
                     /translation="DYTVTFSYVGAGDGAYRNLGGNRFLYAGAGNGDYLPVVALAAPE
                     RTDQYVARGRWRDRALGEVTAEYRRSEQDRNLFSPRGDGDNGGNLWALGYARPWGEPG
                     RGDGITLQSRFREGTFRTRERLYEPEFNRRNHLPDDYEPASDEWRHEGTASFSPAEAV
                     TLAPSGSLVRCDDGTEAESGGMRATVRPGRGVEVAGGVRSATTTVVGAGRRSDGAAQN
                     IDGGVQYEAGKGWRIETGAERDERTNSYAGPRRGTRFVRYRLAAGQRTEQVQAEHYVE
                     DTLTGNWREALRRTRLELRSTRRIRTLAYQATAAYQWLDQPETREESFLGRLNLQYAD
                     PGRRVMAAATYLLSDETRNVRGLSYLEVEPGRGDYVWEDGQYVPDPNGNYVRVEEILS
                     DRAAVSRGEKSFSFSKDWDGLLVRFTSRTEEELLAQGKRTALWVLPFYSDPDEAYQFY
                     SSRYAAEVRLIPIANAYAVNVEASENREVRTIAGSNRERRDRRGTVTFRQAAGQRSFE
                     EAVTLFDADRDAYYSGGGVIEGWQAEAAIRQLIGLHEVSGGSGYRRASGAGGEESRQY
                     LLRGGLRLAVVSRGEVRSTIELYRQELSGVAGLPSYTLTDSRYGRRGAIWSLEFRYGV
                     REDFRVSAAIDGRHSDDRTARVTARGEMVAGF"
BASE COUNT          529 a         1481 c         1038 g          547 t
ORIGIN      
        1 taaaaccccg agcggacttc gatcgacagc cggggctcgc ggaaagggac ttccggattc
       61 caccccagcg ccaccgtcac ggcccgcact ccgtcggtaa gcgccacccc gcccccgaat
      121 cccgaccgat acgactcctc cgttcgcccc cccccggccg cggacacacg gttgctcaga
      181 aaggccccgt cgtagaaccc gaatgcatac cccgccccga accgtatgcg cggttcgatc
      241 gtcgccagca ccgcccgcag cgccgcgaac tgctcgttcc gatacccgcg cagcgtcccc
      301 ggacccccga tcaggttgag ctcggccggg ggcggaaaat cctcgctcgt ctcaaacccc
      361 cggtatccca gcccggcgca cgccgccagc ggccccgcga tccgccggta gacccgggcc
      421 tcgaacaccg tccgcgtctc gccgtactgc gtccgctctc cctccccggc cgcgccgcgc
      481 gcatacttcc ggtgtccata gctgatcgtc gtccgggcct cgaaaccgtt ggccgggttc
      541 agcggctcat cgacgcggtc ccagcgggcg ctgaactccg ccgcgtacgc atcataggac
      601 cgccctccct cggccggctc cactccgcgg tactccagat cgacccccgc cgttaccccc
      661 tgcgccagcc ggactgcgta tcccgcgcgt agcgcgaact cgtaaaacaa cgtccggtaa
      721 tcccgcgtcg ccgcttcgat ccgcccctcc gcccggccgg cgaagaacag cggctgcagg
      781 tagctcacct gcaggagccg gcggtccttg tcccgccgct ccgacagaac ccgcgcccgg
      841 cggcccccgc cgaacaggtt gttcagccgc gcatcaacgt gccacacgac cgtcgcctcg
      901 gatgtatcgg ggatatatcc cccacccaca tcgagccgga gcggccgctc ttccacgaac
      961 tgcagctcca catccgccga ggtaaacccc ggaagcggcc gcacggtggc cgggggaagg
     1021 aagcgcagcc acgggatctc cgctgcctcg cgctccaacc gcacgaggta gcgatcggtc
     1081 aggctgtcgc cggcgttcgc ggtcagatag cgcactgcag tcgcgggctt cgtgcgccgt
     1141 aatcccaggt aatgcacttc cccgagccgc atcacgggtc cggggacgag gctcatgtat
     1201 aacatgatgt catcgccgga tcgctctatc ccgcttattg ttatcgaagc atagtagtag
     1261 ccgcattcgc gctgccggtc cagcagcgaa tcgatcaggc gcgtcacctc ggcttcccga
     1321 aaccacgcct ccaatcgaag cgccgtggtg tccgctccgc tgatcaccgt cgccacccgc
     1381 aaccgggctc ggtccccggc ccgcacccgc acccggccct cctcctggct caccgccgca
     1441 tccatgtaac cttccgtctg cagcatgcgc tccagcgagt ccagtgacac tgccggctgg
     1501 cgcgccgccc gctcgacggc ggcggcgttc accggtcggg gcccttcgaa ccccgcctcc
     1561 cgggccggcg aggcggccgc cccggccgcc agccagagcc acacccacgc gcgcgccatc
     1621 ttcatcagaa ccccgccacc atttcaccgc gggcggtcac ccgcgccgtt cgatcatccg
     1681 agtgccgccc gtcgatcgcc gcactcaccc ggaaatcctc ccgcaccccg tagcggaact
     1741 cgagcgacca gatcgccccc cggcggccgt accggctgtc cgtgagcgta tacgagggca
     1801 gccccgccac tccgctcagc tcctggcgat acagctcgat ggtcgaacgc acctcccccc
     1861 ggctgaccac cgccagccgc aggcctccgc ggagaaggta ctgccgcgac tcttcgccgc
     1921 ccgctcccga ggccctccga tatccgctcc ctccgctcac ctcgtgaagg ccgatcaact
     1981 gccggattgc cgcctcggcc tgccagccct cgatcacccc cccgccggag tagtaggcat
     2041 cgcgatcggc gtcgaacagc gtcaccgcct cctcgaaact cctctgcccc gccgcctggc
     2101 ggaaagtcac cgttccccgg cggtcgcggc gctcccggtt gctccccgcg atcgtccgca
     2161 cctcccggtt ctcactcgcc tccacattga ccgcgtaggc gttcgcgatc gggatcagcc
     2221 gcacctccgc ggcgtagcgc gagctgtaga actggtacgc ctcgtcggga tcggagtaga
     2281 acggcagcac ccagagcgcc gtgcgttttc cctgcgcgag gagttcctct tccgtccgcg
     2341 aggtgaaccg cacgagcagg ccatcccagt ccttgctgaa cgagaagctc ttctcccccc
     2401 gcgacacggc cgcccggtcc gacaggattt cctccacccg gacatagttc ccgttcgggt
     2461 cgggcacata ctgcccgtcc tcccacacgt agtccccccg gcccggttcc acctcgaggt
     2521 aagacagccc gcgcacattc cgcgtctcgt cggagaggag gtaggtcgcg gccgccatca
     2581 cgcgccggcc cgggtcggcg tactggaggt tcagccgccc gagaaacgac tcctcccgag
     2641 tttccggctg atccagccac tggtaggcgg cggtggcctg gtaggcgagt gtccgaatgc
     2701 ggcgggtcga gcgcagttcc aaccgcgtgc gccggagcgc ctcacgccag ttcccggtca
     2761 gcgtgtcctc gacatagtgc tccgcctgga cctgctcggt ccgctggccg gccgccagcc
     2821 ggtagcgcac aaaccgcgtg ccgcggcgcg gcccggcgta cgaattcgtg cgctcgtccc
     2881 gctccgctcc ggtctcgatc cgccatccct tgcccgcctc atactgcacc ccgccgtcga
     2941 tgttctgcgc cgcgccgtcc gagcgccggc cggcgcccac caccgtcgtc gtcgccgacc
     3001 ggacgccccc cgcgacctcc acgccgcgcc ccggccgcac cgtcgcccgc atccctccgc
     3061 tctccgcctc ggtcccgtcg tcgcagcgga ccagcgaccc gctcggcgcg agcgtcaccg
     3121 cctcggcggg actgaaactc gccgtgccct cgtgccgcca ctcgtccgat gccggctcgt
     3181 agtcgtccgg caggtggttt cggcgattga actccggttc gtagaggcgc tcgcgcgtac
     3241 ggaaggtccc ctcgcgaaag cgcgactgca gggtgatccc gtctccgcgc cccggctctc
     3301 cccatggccg cgcgtaaccc agcgcccaga gattcccccc gttgtcgccg tccccccgcg
     3361 ggctgaagag attgcggtcc tgctcgctgc ggcggtactc ggcggtcacc tcgccgagag
     3421 cgcggtcccg ccatcgcccc cgcgcgacat actggtcggt ccgctccggc gccgccagcg
     3481 ccaccaccgg gagatagtcc ccgttccccg cgcccgcata gagaaaccgg tttccgccca
     3541 ggttccgata ggcgccgtcc ccagccccga catagctgaa cgtcaccgtg taatc
//
LOCUS       DAOOKS010000116         3563 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_31737_length_3563_cov_3.693273, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000116 DAOOKS010000000
VERSION     DAOOKS010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3563
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31737_length_3563_cov_3.693273"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1931
                     /locus_tag="PK112_02750"
     CDS             <1..1931
                     /locus_tag="PK112_02750"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HPC10999.1"
                     /translation="YVGEKLTLLAIKLIGELGSYVLLGGSILLLLVLYTSITPVLAKA
                     LPLPGGETVRKIYTVMRNAAVTIFSFRWLVSQSEDEEDEAAAGESEPAAPAPSRERPP
                     DSAEFEDLLDEQIEADAVPDGADPAADREPRPKKPRAKPAASPSDEPVTRGSIDYRFP
                     PLSCLARDPNPANASVTEEELKATSRMLKETLETFGVSVSGEIEYYPGPVITRFELKP
                     AAGVKVNQIVNLSDDLALALKAKRIRIIAPIPGKAAVGIEIPNRKAQLVYLRDILEDD
                     IFARTTMRLPLALGKTTAGKPYAVDLAKLPHLLIAGATGSGKSVCANVLITSLIYKLH
                     PHQIRFIMIDPKMLELTAYSGIPHLGRPVVTNPKRAEKVLSDAVVEMEQRYRRLAAAS
                     VRNIEDFNRKQEADDQRLPYIVILVDELADLLMSSTSSRMELLITRLAQMARAVGIHL
                     VLATQRPSVDVITGLIKANFPARLAFQVSSKVDSRTIIDANGAEKLLGNGDMLFLYPG
                     QPEPIRIHGAYLTGDETEAIVTFIKAQELPMLKLENISQATGEAADVEEVDAGDPLFR
                     EACEVVIRHKQGSVSLLQRRLGIGYQRAARLIDKLEKAGVVSPFDGSKAREVLVDKSY
                     LETLAATEGAPVAEQETN"
     gene            1958..2563
                     /locus_tag="PK112_02755"
     CDS             1958..2563
                     /locus_tag="PK112_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HPC11000.1"
                     /translation="MTKVLISVAVLACSAAAFASDDPFEGAKQDLARAACVQVQFLSV
                     LESDVFDVVDSVPGTAIIAADGRFAVSVGSEEYLYDNERLYTYSAQTDQVIIEKPSGD
                     LFVGNEIALVTRLDRYYRTAPVAAGREYRLTRTDTTGTQLPDELTVLLRPDSSAIEAF
                     LYRDINGDLNRVVLLRQNLSEACADSAFIPAFPESAERIKL"
     gene            2571..>3563
                     /locus_tag="PK112_02760"
     CDS             2571..>3563
                     /locus_tag="PK112_02760"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HPC11001.1"
                     /translation="MKFYIHKLGCPKNDVDADYIAARLIDAGHEPVCDPLAADSIIVN
                     TCGFILPAKEESITEILRLGQLKKEGRLRALFASGCLSQRYGDELLKEIPELDGAFGH
                     GALDSLAAAVDGAPRFDRAVRVEPRRLAYLDWRDRFISDPYPWAYLKISDGCDRGCTF
                     CAIPGMRGRFRSRPLESILREARFLADRGKRELILVSQEATLWGYDLPGRPDILSLLR
                     ALDGLAGVRWIRLMYLYPAALTTELIEYLADDGTKALDYFDLPLQHISDDILRAMRRR
                     GDRRAIESQLRAIREIAPGGTVRTTLIVGFPGETERHFAELREFVERFEFDRLGV"
BASE COUNT          616 a         1230 c         1067 g          650 t
ORIGIN      
        1 gctacgtggg ggagaagctg accctcctgg cgatcaagct catcggcgaa ctcggctcgt
       61 atgtcctttt gggcggctcc atcctgctgc tcctggtgct gtacacttcg atcacccccg
      121 ttttggccaa ggctctgccc ttgccggggg gggagacggt gcggaaaatc tacaccgtca
      181 tgcgcaacgc cgccgtgacg atcttctcgt tccgctggct cgtgtcccag tcggaggatg
      241 aagaggacga ggccgcagcg ggggagagcg agcctgccgc tccggcgcct tcccgcgagc
      301 gtccccctga cagcgcggag ttcgaggacc tcctcgacga gcagatcgag gccgatgccg
      361 tcccggacgg ggctgatccc gctgctgacc gtgaaccgcg tccgaaaaag ccgcgcgcga
      421 agccggcggc gtcgcccagc gatgagccgg tgacccgcgg ctccatcgac taccgttttc
      481 cgccgctttc gtgcttggcc cgggacccca acccggctaa tgcgtcggtc accgaagagg
      541 agctcaaggc gacctcgcgt atgctgaaag aaacacttga gacatttggc gtaagtgtat
      601 ccggggaaat agaatactat cctggtccgg tgattacgag gttcgagctc aaaccggccg
      661 ccggggtgaa ggtcaaccag atcgtcaacc tgtccgatga cttggcgctt gccctgaaag
      721 ccaagcgcat ccgtatcatc gcacccatcc ccggcaaagc ggcggtgggg atcgaaatcc
      781 ccaatcgcaa agcgcaactg gtgtacctgc gcgacattct tgaagacgac attttcgccc
      841 ggacgaccat gcggctgccg ctggcgctcg gcaagaccac ggccggcaag ccctacgccg
      901 ttgatctggc caagctcccc cacctcctca tcgccggggc caccggttcg ggcaagtcgg
      961 tgtgcgcgaa tgtcctgatc acctcgctga tctacaagct ccacccgcac cagatccggt
     1021 tcatcatgat cgatccgaag atgctcgagt tgaccgccta ctcgggcatt ccgcacctcg
     1081 gccgcccggt cgtaaccaac cccaagcggg ccgagaaagt gctctccgat gccgtggtcg
     1141 agatggagca gcgctaccgc cggctggccg ccgcttcggt ccgcaacatt gaagatttca
     1201 accggaaaca ggaggccgat gaccagcgcc tgccgtacat cgtgattctc gtcgatgaac
     1261 tggccgacct cctgatgtcg tcgacttcct cgcgcatgga actgctgatc acccgcctgg
     1321 cccagatggc gcgggcagtc ggcattcacc tcgttctggc cactcagcgt ccctcggtcg
     1381 acgtcatcac cggcctcatc aaggccaact tcccggcccg cctggctttc caggtatcgt
     1441 ccaaggtcga ttcccgcacc atcatcgacg ccaacggggc ggagaagctc ctcggcaacg
     1501 gcgacatgct cttcctgtat cccggccagc ccgaaccgat ccgtatccac ggcgcctacc
     1561 tcaccggcga cgagaccgag gcgatcgtca ctttcatcaa ggcccaggaa cttcccatgc
     1621 tcaagctgga gaacatttcg caggccacgg gcgaggcggc cgatgtcgag gaagtggatg
     1681 ccggcgatcc gcttttccgc gaggcctgcg aagtggtcat ccgccataag caggggtcgg
     1741 tctcgctgct gcagcgccgc ctcggcatcg gttaccagcg ggccgcccgt ctcatcgaca
     1801 agctcgaaaa ggccggcgtc gtctcgccgt tcgacgggtc caaggcccgc gaggtgctgg
     1861 tcgacaagag ttacctggag actctggccg ccaccgaggg ggcgccggtc gccgaacagg
     1921 aaacaaacta atcccccgct cggtcgtata aatggcaatg actaaagtgc ttatctctgt
     1981 cgcggtgctc gcctgctccg cggccgcgtt cgcctccgat gatcctttcg agggcgccaa
     2041 gcaggacctc gcgagagctg cctgcgtgca ggtccagttc ctctccgtgc tcgagtcgga
     2101 cgttttcgac gtcgtcgact ccgtccccgg cacggccatc atcgccgccg acggccgctt
     2161 cgcggtctcg gtcggctccg aggaatacct ctatgacaac gagcgcctct atacctactc
     2221 cgcgcagacc gaccaggtga tcatcgagaa gccctcgggc gaccttttcg tcggcaacga
     2281 aatcgcgctc gtcacgcggc tcgaccggta ctaccggacg gcgccggtcg ctgccggacg
     2341 ggagtatcgc ctgacgcgga cggacacgac cggcacgcag ctgcccgatg agctgacggt
     2401 tctgctccgg cccgattcct ccgcgatcga agcgtttctc tatcgcgaca tcaacggcga
     2461 tctcaaccgg gttgttttgt tgcgccagaa cctgtccgag gcctgcgccg acagcgcgtt
     2521 cattcccgcc tttccggagt ccgcggagcg catcaagttg tagccgcgcc atgaagttct
     2581 acattcacaa gctcggctgt ccgaaaaacg acgtcgatgc cgactacatc gccgcccgcc
     2641 tcatcgacgc cggccacgag ccggtctgcg atcccctcgc cgccgactcg atcattgtca
     2701 acacctgcgg gttcatcctc ccggccaagg aggagtcgat cacggaaatc ctgcggctcg
     2761 ggcagctcaa gaaggagggg cggcttcgcg cgctgttcgc cagcggctgt ctgtcgcagc
     2821 gctacggtga cgagctgctg aaagaaatac cggagctgga cggcgcgttc gggcacggcg
     2881 ctttggattc gctcgccgcc gcggtggacg gcgccccccg gtttgaccgc gccgtgcgcg
     2941 tcgaaccgcg ccgcctggcc tacctggact ggagggaccg cttcatctcc gatccgtacc
     3001 cctgggcgta cctgaaaata tccgacggct gcgaccgggg ctgcaccttt tgtgccatcc
     3061 ccggcatgcg cggccgcttt cgcagccggc cgctcgagtc gattctgcgc gaggcccggt
     3121 ttctggccga ccgcggcaaa cgcgaactga tcctcgtctc gcaggaagcg accctctggg
     3181 ggtacgacct tcccggtcgg cccgacatcc tcagcctcct gcgcgccctt gacggccttg
     3241 cgggggttcg ctggattcgc ctcatgtacc tctacccggc ggccctgacg acagagttga
     3301 tcgagtatct tgccgacgac ggcaccaagg cgctcgacta ctttgatctg ccgctccagc
     3361 acatcagtga cgatattctt cgcgccatgc gccgccgcgg cgaccgccgc gcgatcgaga
     3421 gccaattgcg cgccattcgc gagattgcgc cgggcggcac cgtccgcacc actctcatcg
     3481 ttggtttccc cggcgagacg gaaaggcact tcgccgagct gcgcgagttt gtggagcgct
     3541 tcgagttcga ccgcctcggc gtt
//
LOCUS       DAOOKS010000117         3562 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_31750_length_3562_cov_3.245794, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000117 DAOOKS010000000
VERSION     DAOOKS010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31750_length_3562_cov_3.245794"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="PK112_02765"
     CDS             complement(<1..752)
                     /locus_tag="PK112_02765"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HPC11002.1"
                     /translation="MDRIGIIEQIRRNDRLLSIPQVLSKVLEEVGKENFSPDSLAKVI
                     LNDPNLTSRILHLVNSPFYHRLAEIKTVNQAISVMGVTTVKCLALSTSIFHPDKVSAE
                     SGIDAKAFFQYELAVAAAAEQTAKAVKYRSPEEAMIAGLLQDIGVLFFLHHYPREYRR
                     VAQRKDKSCTLAEAEREVFSIDHTEVGGYLAEAWKLPEYIRAAIREHHETAMVGEQQT
                     LSNIVRMAVLLTNDHFSGYEPRIEQRLEDINRV"
     gene            complement(844..>3562)
                     /locus_tag="PK112_02770"
     CDS             complement(844..>3562)
                     /locus_tag="PK112_02770"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPC11003.1"
                     /translation="PYLDLAHFAVILAGLLLAAAGLAFYADGWQTRREDIEVRDQKLS
                     ILDNLQRDARGPYQLVDLLSMSIREIVSHLPDAAGAVFLLNRKHRQFVLTASVGLSQE
                     ETALLEYFPLQRNTVSQSIDIGEPILGRSFSFIERSGAERPSRFQSVLVLPLVSGMEK
                     IGGILLVAEAEHYFSRAEIRYLQPVAEWIAEKIKSARLERELTAAVRQSEERAQRGDD
                     LATRLRAATAALGARDGVAAFCRALAGVAESRSVHLFGLTGGALQFYGGSESLGDISE
                     NYRTALVDALDRNKPLVINQEGVGDGGRKFVAVSSLVAPLGGREASALLLRREDGPFR
                     VDDDDLKQLELFGGLARLALQRADTERLDLTRRKGFETVLQLLRFDVDVEPDKQPDFF
                     TAHMAPALPAGSLAVLFRRENDGSFTAVGGAPGAADLRLLPGEGIVGEAANRAEGRFV
                     FSRAAVGRAIEALESGSREQMQALFGEQGVPGFAAACPILRLDRVAAVALFLLYDATE
                     SERGEWERLLTLASSLYSVRLTVAELNRRVLEAQTTGGGVDQDLVGRMVNELNNHLSA
                     VIGNAELAFTRTNLTGDVAQHLRSVIAEAEQAAKALKDSLGRMRLPAPSTAVAAVEDK
                     SLGAVVKAVLRRLQVAENLYLAGGRAREMAVLAEADGVVPVAEERLYGLVEEMLNRFG
                     AAAADDDVLTLRLYRSDDRLCLDLSRHHRNFPPVDKVAGFGEYEGAPEALRQRPSDTF
                     LRYLADSDVTYAHDRHTQPSTYLSFQFPTAAPGGSARAARPKEAVRILAIDDQTVILD
                     LIAAMSQSMGYEVAVASSGPEGIALARRQRFTLVLTDLAMPGMSGLEVAAEIRKLQPD
                     TPIVLVTGWEVTTDRAQLEEAGIVRVLYKPFRIEQLTEIIRSAVQISPI"
BASE COUNT          608 a         1306 c         1073 g          575 t
ORIGIN      
        1 acccggttga tgtcctcgag ccgctgttcg attcgcggct cgtacccgga gaaatggtcg
       61 ttggtgagca gcaccgccat ccggacaatg ttcgagagcg tctgctgctc cccgaccatc
      121 gccgtctcgt ggtgctcgcg aatggccgcc cggatatact cgggcagctt ccaggcctcc
      181 gcaaggtacc ccccgacctc ggtgtggtcg atgctgaaca cctcccgttc ggcctccgcc
      241 aacgtgcagc tcttgtcctt ccgctgcgcc accctgcggt actcccgggg atagtggtgg
      301 aggaagaaca gcaccccgat gtcctggagc agaccggcaa tcatcgcttc ttcgggcgac
      361 cggtacttca ccgccttcgc cgtctgctcg gcggccgcgg cgaccgccag ctcgtactgg
      421 aagaacgcct tggcgtcgat ccccgattcg gcggagactt tgtccgggtg gaagatcgag
      481 gtggagaggg ccaggcactt gaccgtcgtg acccccatga ccgaaatcgc ctggttgacc
      541 gtcttgatct ccgccaggcg atgatagaaa ggcgaattga ccagatggag aatccgactg
      601 gtcaggttcg gatcattgag aatgactttc gccagcgagt ctggagagaa gttctccttc
      661 cccacctctt ccagcacttt cgagagcacc tgcggaatgg acaacaaccg gtcgttccgg
      721 cgtatttgct cgataatccc gattcgatcc atacgcggat aatcctttcc ctgtcatact
      781 accgttatcg gcttgggaat tcaggagtta accgcgagcc ccgggaaacc ggaaatatcg
      841 aactcagatc ggtgagatct gcacggccga ccgaatgatc tctgtaagct gttcaatccg
      901 aaagggttta tagaggaccc ggacaatccc tgcctcctca agctgcgccc ggtcggtcgt
      961 cacctcccac cccgtgacca acacgatcgg cgtgtccggc tgaagcttgc gaatctccgc
     1021 cgccacctcc aggccggaca tccccggcat cgccaggtcg gtcagcacaa gcgtgaaccg
     1081 ctgccgccgc gccagcgcga ttccctccgg ccccgaggac gccaccgcca cctcgtagcc
     1141 cattgactgg ctcatggccg cgatcagatc gagaatgact gtctggtcgt cgatcgccag
     1201 aatccggacc gcttccttcg gacgggccgc ccgcgccgac cctcccggcg ccgccgtcgg
     1261 aaactggaac gacaggtaag tcgagggctg ggtgtggcgg tcgtgggcgt aggtgacgtc
     1321 gctatcggcc aggtagcgca ggaacgtatc cgatggccgc tggcggagcg cctctggggc
     1381 gccctcgtac tcgccgaagc cggccacctt gtcgaccggc gggaaattgc ggtggtgccg
     1441 cgagagatcg aggcacagcc ggtcgtccga ccgatacagg cgcagcgtga gcacatcgtc
     1501 gtcggccgcc gccgccccga accggttgag catttcttcg accagtccgt agaggcgttc
     1561 ctcggccacc ggcacgacgc cgtcggcttc ggcgagcacg gccatttcgc gcgcccgccc
     1621 gccggccagg tagagatttt cggccacctg caggcggcgg agcaccgctt tcaccaccgc
     1681 cccgagcgac ttgtcctcga ccgccgccac cgccgtcgac ggcgccggca gccgcatccg
     1741 tcccagcgaa tccttcagcg ctttcgccgc ctgttcggcc tcggcgatga cgctgcggag
     1801 atgctgcgcc acatcccccg tgagattggt ccgcgtgaaa gcgagttcgg cgttcccgat
     1861 cacggcggag aggtggttgt tcaactcgtt gaccatccgc cccacgagat cctgatccac
     1921 gccgcccccc gtcgtctgcg cctccagcac ccgccggttg agctcggcca ccgtcagccg
     1981 caccgaatac agcgaggagg ccagggtgag cagccgctcc cactctcccc gctcgctctc
     2041 ggtcgcgtcg tacaggagaa agagggccac cgcggccacg cgatcgagcc tcaaaatggg
     2101 gcaggccgcg gcgaagcccg gcacgccctg ctcgccgaag agcgcctgca tctgctcgcg
     2161 cgatcccgat tccaaagcct cgatcgcccg tccgaccgcc gcccggctga acacgaaccg
     2221 cccctcggcg cggttggccg cctcccccac gatcccctcg cccggcagca agcggagatc
     2281 cgccgccccc ggcgcgccgc cgacagcggt gaacgagccg tcgttctccc gacggaacag
     2341 gacggccaga ctccccgccg gcagcgccgg ggccatgtgc gccgtgaaga aatcgggctg
     2401 cttgtcgggc tcgacgtcga catcgaaccg cagcaactgc agcaccgtct cgaacccctt
     2461 ccgccgcgtc aggtcgagcc gctcggtgtc cgcccgctgg agcgccagcc gggcgagacc
     2521 tccgaacagc tcaagctgtt tgagatcgtc atcgtcgacc cggaacggcc cgtcctcccg
     2581 ccgcagaagg agcgcgctcg cctcccgccc gcccagcggc gccacgagac tggacacggc
     2641 cacgaatttc cgcccgccgt cgcccacccc ctcctggttg atcacgagcg gcttgttgcg
     2701 gtcgagcgca tccaccagcg ccgtccggta attctccgaa atgtccccga gcgattccga
     2761 cccgccgtaa aactgcagcg ctccgccggt cagcccgaag aggtggacgg agcggctctc
     2821 ggcgacgccc gcgagcgccc ggcagaaagc cgccaccccg tcgcgcgcgc ccagggccgc
     2881 cgttgccgcc cgcagacggg tcgccaggtc atccccccgc tgcgcccgct cctcgctctg
     2941 ccgcaccgcc gctgtcagct cccgctccag ccgcgccgac ttgatcttct ccgcaatcca
     3001 ctccgccacc ggctgtaaat accggatctc cgcccggctg aaatagtgct ccgcctcggc
     3061 caccagcagg atgcccccga tcttctccat ccccgacacc agcggcagca ccagcaccga
     3121 ctgaaagcgc gagggccgct ccgcccccga acgctcgatg aagctgaaac tccgaccgag
     3181 aatcggctcg ccgatatcga tactctgact gacggtattc cgctggagcg ggaaatactc
     3241 cagcagcgcg gtctcctcct gggagagccc gaccgaggcg gtgagtacga actgccggtg
     3301 cttccggttg agcaggaaca ccgcccccgc cgcgtccgga aggtgcgaca cgatctcccg
     3361 gattgacatg gagagcaggt ccacgagttg gtacggtccg cgcgcgtccc gctggaggtt
     3421 gtcgaggatc gacagcttct ggtcgcgcac ctcgatgtcc tcgcgccgcg tctgccaccc
     3481 gtcggcatag aacgccaggc ccgccgccgc caggagcagc ccggcgagga tcacggcaaa
     3541 gtgcgcgagg tcgagatagg gg
//
LOCUS       DAOOKS010000118         3557 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_31812_length_3557_cov_3.329240, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000118 DAOOKS010000000
VERSION     DAOOKS010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31812_length_3557_cov_3.329240"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1413)
                     /locus_tag="PK112_02775"
     CDS             complement(<1..1413)
                     /locus_tag="PK112_02775"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HPC11004.1"
                     /translation="MKSMLIAALLLLAPAAPAVRAAYLITADSLRAHEMVLAHDSLEG
                     RRVGEEGEMKAARYIESAFAAAGLQPRGTDGSWLQPFEFVRAIELGPGNRLVLNGAPL
                     ALHGEFEPMKQSASLAFDFDTIVDVGYGITVEEADGNYDDYAGKDVAGKAVLIKRYAP
                     SADENPHVDFEPYSSLTSKINTALEHDAAGIFFITPEDQDDTLQPIGPVRITPKDIPI
                     IFLRRAGFEHLGLSLSDPAVLSAEGAVELVKVHDTGYNVLGYLPGETDTTVIIGAHYD
                     HLGSGGPGSGSRYLGNDPQIHNGADDNGSGTSVLLEIARHYAAQAEKPHYSLLFAAFS
                     GEEFGILGSSHFARDMTVDSAKVRMMVNMDMVGRLAEQDNGLAVMGTGTTEAFTVYFD
                     SLRREDVKITQKKSGQGPSDHTAFYNRGIPVLMFFTGAHNDYHKPEDDWDRIDYAGLV
                     TVADVVTDLVSYFDRYPGPLT"
     gene            1638..2336
                     /locus_tag="PK112_02780"
     CDS             1638..2336
                     /locus_tag="PK112_02780"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPC11005.1"
                     /translation="MTDSICAACGKPLTAGDRFCAACGAPTDLDVKPVRGGKPASKKP
                     PAGAPRSASTGMRDGAIIVGALALITVGWFVFRTPAVPPQPPSAATPAGHPEVPGAPM
                     GGMTGGLSDLPADYDGLVQAGNRYMDAGNYAVAAQSYRRALALDPSSPDVRTDFGACL
                     HGMGLPERAIEEFREVMQTHPEHGIANFNIGIVYLDMGKPDSARFYWEKYLQLEPTGA
                     AAAKARELLGNLNG"
     gene            2329..3456
                     /locus_tag="PK112_02785"
     CDS             2329..3456
                     /locus_tag="PK112_02785"
                     /inference="COORDINATES: protein motif:HMM:NF033936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SO_0444 family Cu/Zn efflux transporter"
                     /protein_id="HPC11006.1"
                     /translation="MDSAATFLGNWAEATWAMVVDSALLLLLSFVLAGLLHLVLNART
                     IGKLVSGGRRREVFATALVGVPLPLCSCSVLPVAYQLRRAGVSKAGTTAFLIATPESG
                     VDSVLLTWSLMDPLMTVARPVAAFLTAFTAGNIEAAFPDDRPTGKDGAAAGGDGCACG
                     CAAEPNAAVAPVRIADRAPRASWLSRVLAGIRYGFTDLFSDLAVYLLVGYLLAGLVAA
                     VFGSALLSLPPWLTAGWGGYAGAIVIGLPLYICATSSTPLAAALLGAGFSPGAVLVFL
                     LVGPATNVASMTVVGKILKGWGLARYLGSIVIVAVLCGIALDWAYRTVGIAAAFRAGG
                     HDHASWYHVAAGLLFVLLLARVLAMKIRRRLRRGRMTPAAG"
BASE COUNT          547 a         1138 c         1220 g          652 t
ORIGIN      
        1 tgtcagcggg ccgggatagc ggtcgaaata ggaaacgaga tcggtcacga cgtcggcgac
       61 cgtgacgagg ccggcgtagt caatcctgtc ccaatcgtcc tccggcttgt ggtagtcgtt
      121 atgggcgccg gtgaagaaca tgaggacggg aatgccgcga ttgtagaacg cggtgtggtc
      181 ggagggccct tggccggact tcttctgcgt gatcttcacg tcctcacgcc tgaggctgtc
      241 gaagtagacg gtgaaggcct cggtcgtgcc ggtccccatc accgccagcc cattgtcctg
      301 ttcggccagc cgcccgacca tgtccatatt gaccatcatg cgaaccttcg cgctgtcgac
      361 ggtcatgtcc cgggcgaaat ggctcgagcc gagaatgccg aattcctcgc cgctgaaggc
      421 cgcgaaaagg agagaatagt gcggtttctc ggcctgcgcg gcatagtggc gggcgatctc
      481 cagcaggacc gaagtccccg atccgttgtc gtcggcgccg ttgtgaatct gcggatcgtt
      541 gcccagatag cgcgagccgc ttccggggcc gccagacccg agatgatcgt agtgggcgcc
      601 gatgatgact gtcgtgtcgg tctcgcccgg caggtagccg agcacgttgt agccggtgtc
      661 gtggaccttg accagttcca ccgccccttc ggccgacagg acggccggat cgctcagtga
      721 gagccccagg tgctcgaatc cggcgcggcg caggaagatg atggggatgt ctttcggcgt
      781 gatccggacc gggccgatcg gctggagcgt gtcgtcctga tcctcggggg tgatgaagaa
      841 gataccggct gcgtcatgtt cgagcgcagt gttgatcttg ctggtgagcg aactgtatgg
      901 ttcaaagtcc acgtgcgggt tttcatccgc agatggagcg tagcgcttga tcagcacggc
      961 tttgccggcg acatctttgc ctgcgtaatc gtcgtaattg ccgtcggcct cttcgaccgt
     1021 gatgccgtag ccgacgtcca caatggtgtc gaagtcgaac gcgaggctgg ccgactgctt
     1081 catcggctca aactccccgt ggagcgccag gggcgcgccg ttgaggacga ggcggttgcc
     1141 cggaccaagc tcgattgccc tgacgaactc gaaaggctgc agccaggaac cgtctgtccc
     1201 gcgcggctgg agcccggccg cggcgaaggc ggactcgatg tagcgggcgg ctttcatttc
     1261 cccctcctcg ccgacccggc gcccctcgag ggaatcatgg gcaagaacca tctcgtgggc
     1321 ccgcagggaa tccgcggtga tcaggtaagc ggcccggacc gccggagcgg ccggcgccag
     1381 cagcagcagc gcagcgatga gcatactctt catacaactt ccttataccc ggcaagcgcc
     1441 aaaacctcgc actcccggcg ggcggcatca acccgccgtc ggccgccgac agagaacagg
     1501 ggggacaata tcgcttgcca atatacgaat tgtcacgcgc ttgtcacggc ccttgccgaa
     1561 ccgcgggcac tttttgattg ccgcgcggtt aaggtggatt atattgcccg ctgaactata
     1621 caaaggaaag caacggcatg acggacagca tttgtgcagc ttgcggcaaa cccctcacgg
     1681 ccggggaccg tttctgcgcc gcctgcggcg cgccgaccga ccttgacgtt aagccggtcc
     1741 gcggcgggaa acccgcctcg aaaaagcctc ccgcgggcgc gccgcgcagc gcctccaccg
     1801 ggatgcgtga cggcgcgatc attgtcgggg cgcttgcgct gatcacggtc ggctggttcg
     1861 tgtttcggac ccccgccgtt ccgccgcagc cgccctccgc cgcgacgccg gcgggccacc
     1921 cggaggtgcc gggcgccccg atgggcggga tgaccggagg tctgagcgat ctgccggccg
     1981 actacgacgg actcgtgcag gcggggaacc ggtacatgga cgccgggaac tatgcggtgg
     2041 cggcccagag ttatcggcgg gcgctggccc tcgatccgtc gtcgcccgac gtgcgcaccg
     2101 atttcggcgc ttgcctccac ggcatgggac tgcctgagcg ggccattgag gagttccgtg
     2161 aggtgatgca gacccacccc gaacacggca tcgccaattt caacatcggc atcgtctatc
     2221 tcgacatggg caagccggac tcggcccgct tctactggga aaagtatctc cagcttgagc
     2281 cgaccggggc ggccgccgcc aaagcgcgcg aactgctcgg caacctgaat ggatagcgca
     2341 gcgacttttt tgggcaactg ggccgaggcg acctgggcaa tggtggtcga ttcggccttg
     2401 ctgcttctgc tcagtttcgt cctggccggg ctgctccacc tcgtgctcaa tgcccgcaca
     2461 atcgggaaac tggtctccgg cggacggcgg cgcgaagtgt tcgccaccgc gctggtcggc
     2521 gtgccgctgc ctttgtgctc ctgttcggtg ctgccggtgg cctatcaact ccggcgggcc
     2581 ggcgtgagca aagccggcac caccgccttt ctcatcgcca cgcccgaatc gggcgtcgat
     2641 tccgtcctgc tcacctggag cctcatggat cccctcatga cggtcgcgcg gccggtcgcg
     2701 gcttttctga cggcgttcac cgcgggcaac atcgaggcgg cctttccgga tgaccggccg
     2761 accgggaaag acggagcggc agccggggga gacggctgcg cctgcggctg cgcagcggag
     2821 ccgaacgcag ccgtcgcgcc ggtacggatc gccgatcgcg ccccgcgcgc gtcctggctg
     2881 agccgggtgc tggccgggat caggtacggg ttcacggatt tgttcagcga tctggccgtc
     2941 tatctccttg tcgggtatct gctggcgggt ctggtggcgg ccgtattcgg cagcgcgctg
     3001 ttgtctctgc cgccgtggct gaccgccggc tggggcggat acgccggggc gatcgtgatc
     3061 ggcctgccgc tctacatctg cgcgacctcg tcgacacccc tggccgcggc gctcctggga
     3121 gccgggtttt cgccgggcgc ggtgctggtc ttcctgctgg tggggccggc caccaatgtg
     3181 gcctcgatga ccgtggttgg caaaattctc aaggggtggg gactggcgcg gtacctcggg
     3241 tcgattgtga ttgtggccgt gctctgcggg atcgcgctcg actgggccta ccgcaccgtg
     3301 ggtatcgcgg ccgcgttccg cgccggcggg catgaccatg cctcctggta ccacgtcgcc
     3361 gccggcctgc tgtttgtgct gctgctggcg cgggtgctgg cgatgaagat ccggcggcgc
     3421 ctgcggcgcg gacggatgac accggcggcc ggatgagctc gggcgcggcg cgggcgcagg
     3481 cgggagccct tgccgccggc gtgaccgcaa ggtatcttgg aggcatggcg gatcagacct
     3541 tcccctgcgt gtcgctg
//
LOCUS       DAOOKS010000119         3549 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_31904_length_3549_cov_4.022038, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000119 DAOOKS010000000
VERSION     DAOOKS010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31904_length_3549_cov_4.022038"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1614
                     /locus_tag="PK112_02790"
     CDS             <1..1614
                     /locus_tag="PK112_02790"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPC11007.1"
                     /translation="LAALLPLALAGFGAWIVFGSLFEQRSLESLRGLVRAHARTIDAD
                     LSEHIRLLQVLAASQTRTRLADDAQLDTLLAELNRAGDNSFTDLGVIDAEGDHLAYAG
                     PFDLKGRNYRGDEWFGEAMERGVSLSDVFLGFRGAPHCVIAVRAGNAAEGFWILRGTI
                     NSARFDTLVQAGKCNGGDAYIVSRRGLYQTASRRGALLDTARGIPLVPHEGAADYRAT
                     LAGTDLILLTAWLQSGRWLLVVEQDRTALRAPVNQAITRGAQVVLIAVLILIVTTVIA
                     TRHLCRQIDRANAQREELSRAFVRSARLASIGELATGLAHEINNPLAVISAEHTNLAD
                     LLRASDLPEPTRGDLLESVERCRRQVQRCAGITRKMLQFGRRREAAPEPTDITPRLQE
                     IIALLRRRAGVRNVALHLETAAGLRPVLVDPIEFEQVIVNLINNAVDALPGGGDVWVR
                     AGAAGDRLRIEVADNGVGIPADTLERIFEPFYTTKPVGQGTGLGLSVCYGLVGEWGGR
                     ITAESAVGRGTTMRVELPLPHPDNSTARP"
     gene            1625..2470
                     /locus_tag="PK112_02795"
     CDS             1625..2470
                     /locus_tag="PK112_02795"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPC11008.1"
                     /translation="MHNPYQMIRVLLVDDERDFLASTAQALTRRGFRVTTADSGTAAL
                     RLLEGDFFDAVVLDVKMPDVDGLEVFRQVHGLRPQLPVILLTGYASAAQAAAAAESGV
                     AVYLAKPCEIGDLVEHIRNALGARRVVDENGFESAAMEEVSVLLVDDEDELIAALSPV
                     LRRRGMTVSAASGGEEALRVLRESFVDVVVLDLKMPGMDGLAALRHMKREFPDVEIVI
                     MTGHPSADSAVQTIKAGASEYLMKPPDTDELVGAIRKVFRRRRQRLEQKQQRLIDEVR
                     KRYPE"
     gene            complement(2750..>3549)
                     /locus_tag="PK112_02800"
     CDS             complement(2750..>3549)
                     /locus_tag="PK112_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010235983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HPC11009.1"
                     /translation="EELRSKVDTLITIPNERLLQIVDRKTTLADAFKVADEVLHQATK
                     GISDLITIPGLINCDFADVRTVMLERGDALMGTGWGKGEEKAVDAAQEAISSPLLENV
                     SISGARGVLINVTGGEDMTLFDVNNATSLIYEAAGNNANIIFGAVIDPTMADEMRVTV
                     IATGFGGVETKRAQVEKDERERAAREVPSGAPISLFPESAVARPPEPAPAAEPEEVLA
                     AAGVPEYGDRGGNGNGNGNGKGKPRVPMFADTDRTVPAYLRRLTDNR"
BASE COUNT          513 a         1268 c         1155 g          613 t
ORIGIN      
        1 ctggcggccc tgttgcccct ggcgttggcc ggctttggcg cctggatcgt tttcggcagc
       61 ctctttgagc agcgttctct ggagtcgctc cgcgggctcg ttcgggcgca cgcccgcact
      121 atagacgccg acctttccga gcacatccgt ctcctgcagg tgctggcggc ctcgcagacg
      181 cgcacgcgtt tggccgacga tgcgcaactc gacacccttc tggccgaact caaccgcgcc
      241 ggcgacaaca gcttcaccga cctgggagtc atcgacgccg agggggacca cctcgcctat
      301 gccggtccct tcgacctcaa gggccgcaac tatcgcgggg acgagtggtt cggcgaggcc
      361 atggagcgcg gcgtttccct cagcgatgtt ttcctcggat ttcgcggcgc gccccactgc
      421 gtgattgcgg tacgggccgg gaatgccgcc gagggtttct ggattctccg cggcacgatc
      481 aactcggcgc ggttcgacac gttggtgcag gcgggcaagt gcaacggcgg cgacgcctac
      541 atcgtcagcc gccgcgggct ctaccagact gcgtcgcggc gcggcgccct tctggacacg
      601 gcgcgcggca tcccgcttgt tccccacgag ggcgcggcgg actaccgggc caccctggcc
      661 gggaccgacc tcatcctgct caccgcctgg ctccagtcgg ggcgctggct gctggtggtc
      721 gagcaggacc gcacggcgct gcgcgccccg gtcaaccagg cgatcacccg gggggcgcag
      781 gttgtgctca tcgccgtcct catcctcatc gtcacgaccg tcatcgccac ccgccacctc
      841 tgccgtcaga tcgaccgcgc caatgcgcag cgcgaggagc tgtcgcgcgc gtttgtccgc
      901 tcggcccgcc tagcctcgat cggggagctg gccaccggcc tcgcccacga aatcaacaac
      961 ccgctggcgg tgatcagcgc cgagcacacc aatctcgccg acctgctccg cgcgagtgac
     1021 ctgccggaac cgacccgcgg cgacctgctc gagtcggtcg agcgctgccg ccgccaggtg
     1081 cagcgctgcg ccggcatcac gcgcaagatg ctccagttcg gccgccgccg cgaagccgcg
     1141 cccgaaccga ccgacatcac cccgcgtctc caggagatca tcgccctgct ccgccgtcgc
     1201 gcgggtgttc gcaacgtcgc tctccatctc gagacggcgg ccgggctccg gccggtgctg
     1261 gttgacccca tcgagttcga acaggtcatc gtcaacctca tcaataacgc ggttgacgcg
     1321 ctgcccggcg gcggcgacgt ctgggttcgc gccggcgccg ccggcgaccg cctccgcatc
     1381 gaggtcgccg acaacggcgt cggcatcccg gccgacacgc tcgaacgcat tttcgaaccc
     1441 ttctacacca ccaaaccggt cggccagggg accggcctcg ggctgtccgt gtgctacggc
     1501 ctggtcggcg agtggggcgg gcgtatcacc gccgagagcg ccgtcggccg cggcaccacc
     1561 atgcgggtgg agctgccgct cccgcacccc gacaattcca ccgcgagacc atagagggat
     1621 ccacatgcac aacccgtatc agatgatccg cgtgcttttg gtcgacgatg agcgggattt
     1681 cctcgcgtcg accgcccagg cgctgacccg ccgcggcttc cgggtcacca ccgccgacag
     1741 cggcacggcc gcgctccgcc tgctggaggg ggacttcttc gacgccgtcg tgctcgacgt
     1801 gaagatgccc gacgttgacg gcctggaggt cttccggcag gtccacggcc tgcggccgca
     1861 gctcccggtc attctcctca ccggctacgc ctcggccgcg caggcggcgg ccgccgccga
     1921 atccggcgtc gccgtctacc tcgccaaacc gtgcgagatc ggcgacctgg tggagcatat
     1981 ccggaacgcc ctcggcgcgc gccgggtggt cgatgaaaac ggtttcgagt ccgccgccat
     2041 ggaggaagtc agcgtgctcc tggtcgacga cgaggacgag ctcatcgccg cgctttcgcc
     2101 ggtgctgcgc cggcggggca tgacggtcag cgcggcctcc ggcggcgagg aggcgctccg
     2161 cgttctgcgc gagtcgttcg tcgacgtcgt cgtgctcgat ctcaagatgc cgggcatgga
     2221 cggcctcgcg gcgctccgcc acatgaagcg ggagttcccc gacgtcgaga ttgtcatcat
     2281 gaccggccac ccctcggcgg attcggcggt ccagacgatc aaggcgggcg cgagcgagta
     2341 cctcatgaaa ccgccggaca ccgatgaact cgtgggggcg atccgcaaag tcttccgccg
     2401 gcgccgccag cgcctcgaac aaaaacagca gcgcctcatc gacgaggtcc ggaagcgcta
     2461 tcccgagtga ccgccggcgt tgcggattcg gcggccccgt ctctctcacg aaaaaaaatg
     2521 ccgccggcag ggtagtttgg ccgacggcac tgtggaggaa ccaagtacac aaatagagcg
     2581 cgagccctat ttcctgatgt aacggttctt gggtgcaatg aacaggcgcc ggccgtggag
     2641 cgtgccggcg tccgttccct ggtttatctc tctctcgctg gtctccttcc ttcgcttggt
     2701 ctccggtctt ccgtttaacg tattcgcgtt tcgatagtcg cttctttcgc taccggttgt
     2761 cggtcagccg ccgcaggtac gcgggcacgg tccggtcggt gtcggcaaac atcggcacgc
     2821 gcggtttccc cttgccgttg ccgttgccgt tgccgttgcc gccgcggtcg ccgtactcgg
     2881 ggacgccggc cgcggccagc acttcttccg gctcggccgc gggcgccggt tccgggggcc
     2941 gtgcgacggc gctctcggga aagagcgaga tcggcgcgcc cgaggggact tcgcgcgccg
     3001 cccgctcccg ctcgtccttc tcgacctggg cgcgcttggt ctcgacaccg ccgaacccgg
     3061 tggcgatgac ggtcacccgc atctcgtcgg ccatcgtcgg gtcgatcacc gcgccgaaga
     3121 tgatgttggc gttgttcccc gccgcctcgt agatgagcga ggtcgcgttg ttgacgtcga
     3181 aaagagtcat gtcctcgccg ccggtaacat tgatgaggac gccgcgggcg ccgctgatcg
     3241 agacgttttc caggagcggc gacgagatcg cctcctgggc cgcatccacc gctttctcct
     3301 cccccttccc ccagcccgtg cccatgaggg cgtcgccgcg ctcgagcatg accgtgcgca
     3361 cgtcggcaaa atcgcagttg atcaggccgg ggatcgtgat gaggtcggaa atccccttgg
     3421 tcgcctggtg cagcacctcg tccgccacct tgaaggcgtc cgcgagggtg gtcttgcggt
     3481 cgacaatctg caggagccgc tcgttgggga tggtgatgag cgtatcgacc ttcgagcgca
     3541 gctcttcca
//
LOCUS       DAOOKS010000120         3544 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_31966_length_3544_cov_2.977931, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000120 DAOOKS010000000
VERSION     DAOOKS010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31966_length_3544_cov_2.977931"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="PK112_02805"
     CDS             complement(<1..624)
                     /locus_tag="PK112_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11010.1"
                     /translation="MMAIAMAVAVFTLTGCSGDGKAEKPNQAAPAAEAGAMPSTIAGA
                     ELLGASRPAVTGEKGFPIADMIRVDSTLYAVAGNELIITDLKTKSERRVSAAEGLYAV
                     TEHNGKIYAGGTALYELGETGLEPAAAAFDGTIRALTGHEYRLMIGTSTGLFASSIFG
                     DEALFEGIDVTAMAGDSDELWVGTGGQGLYRWDGAEFQRRYLLRDTAV"
     gene            910..2151
                     /locus_tag="PK112_02810"
     CDS             910..2151
                     /locus_tag="PK112_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11011.1"
                     /translation="MTRCSWIFALPLLLAAVCAAQPGDSLWIESRPLQVKLDRLYLDC
                     GLEARVFPDCRFVVHRGPDSLYSGCIESSYPGVAVSSPVGGFFDTLRLADLRVWLERA
                     PYDTLAPIVLGQVGLLPAPLAGVGDSTAGGRVVFRPYASHFEASLAFEDQVIDGFFSY
                     RRYTAGGEDVASVSRPAPFFVALVPNPARAIAQRGFLSTSLYYRLDPQRLAMIFEGDE
                     LRAFNRCGVGGDTAARPYPYAPGPGRALLNNLRGPSRSVVIAVEHPMFEPAAGYFADI
                     LSRDRFRPTVTSTLAGADLRLVAVPLGESEADGLRCLSGLLALDTLAGSAAAQSLQAV
                     SSCLDSAALAAEPAAALRYVQLAESVLRDDLGVFPLFRPTLYFTARRSLLGEVFDRRG
                     GIDVSGLARVRIPQNRKDCRP"
     gene            2148..>3244
                     /locus_tag="PK112_02815"
     CDS             2148..>3244
                     /locus_tag="PK112_02815"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HPC11012.1"
                     /translation="MTFTGRIRLYLILVAVVPTVLVVSVIYLLGMRQAAAAERRSAQH
                     SLDRFAQYQRAVADELRQAVIAAAATPSAARALLLLKAGRASDVRFDAVPAGLDFFEI
                     ADSSLLVQASARRPGLIGERLPSLDSLAADSAPLASVEYDLNGPHAALAWRLPAESAY
                     VVYAGRYLDSQYTGTVARLIGADVRIVLAGDTGGGLSGMDPGRLYAERDSLVAVLAGG
                     DAAGWFLAASFRSGPEQRVFGNLLVVSGLVALGSVLAAVALGLYISGRAQREFDNLLT
                     ATARVSGGDLSTPVMAYEEGEFAQLADSFTAMTFRLRDAQRRLATTEKIAAWQAVDRK
                     IAHEVKNPLTPIVISTDDLRRDVDKRIRSVIR"
     assembly_gap    3245..3254
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3255..>3544
                     /locus_tag="PK112_02820"
     CDS             <3255..>3544
                     /locus_tag="PK112_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11013.1"
                     /translation="FQEQLPDFPRILEETTATIKSEVERLTRLLDEFVGFARMGPPVI
                     RDVAPARLLEPLAALYRGETGTGRLTLSNRSARRSVRLDPEMMHQVLVNLVK"
BASE COUNT          500 a         1233 c         1127 g          674 t
ORIGIN      
        1 caccgccgtg tcgcgaagca gatagcggcg ctggaactcc gccccatccc aacgatagag
       61 cccctgtccg ccggtcccca cccacagttc atcgctatcc cccgccatag cggtcacgtc
      121 gattccttcg aagagcgcct catcgccgaa aatcgaagac gcgaagagcc ccgtgctcgt
      181 cccgatcatg agccggtact cgtgcccggt cagggcgcga atggtcccgt cgaacgccgc
      241 cgccgccggt tcgagcccgg tctcccccaa ctcgtagagt gcggttccgc ccgcatagat
      301 cttcccgttg tgctccgtca cggcgtacag cccttcggcc gcgctcaccc gccgctcgct
      361 tttggttttg agatcggtga taatcagctc gtttccggcc accgcgtaga gggtcgaatc
      421 gacgcggatc atgtcggcga tcggaaaccc cttttccccg gtcaccgcgg gccggctggc
      481 cccgagcagt tcggctcccg caatggtgga gggcatggca ccggcctcag ccgccggcgc
      541 cgcctggttc ggcttctccg ctttgccgtc gccagagcag ccagtaagag tgaacaccgc
      601 gacagccatc gctatcgcca tcatttcaag aatccttttc gttttcatca ctacctccag
      661 aaattcgagg gttcggttca tgtcgagttc cctcatctca agcgatatgc caaattgact
      721 aacatattgt caatcaatag catacgtgga attccccgtg gtcgatgcca cccctggtct
      781 gtgtctctca tgacacaatt gttgcgcgag cagtcggttg ccgatataat tcaacaggtt
      841 atcgcgtgtc atgcgtgaca cacatatccg gcaggccttc tcacgtgtat acagggcatg
      901 aggtctcagg tgactcgttg ctcatggatc ttcgcgcttc cgctcctcct ggcggccgtc
      961 tgcgcagcgc agccgggcga cagcctgtgg atcgagagtc gccctctgca ggtcaagttg
     1021 gaccgcctct acctcgattg cggactggag gcgcgtgtgt tccccgactg ccggtttgtc
     1081 gtgcatcgcg gtcccgattc gctctacagc ggatgcatcg aatcctccta ccccggggtg
     1141 gcggtcagct ccccggtcgg cggcttcttc gacactctcc gcctggccga tctccgcgtg
     1201 tggctggagc gtgcccctta tgacacgctg gccccgatcg tgctcggcca ggtcggcttg
     1261 ctcccggcgc ccctggccgg cgtcggcgac tcgaccgccg gaggccgcgt cgtgttccgg
     1321 ccgtatgctt cgcattttga ggcttcgctg gcattcgagg atcaggtgat cgacgggttc
     1381 ttctcgtacc gccggtacac ggcgggcgga gaagatgtcg cctcagtcag tcggcccgct
     1441 cccttcttcg tcgccctcgt tcccaacccg gcgcgggcga ttgcccagcg tggttttctg
     1501 tccacgtcgc tctactatcg gctcgatccg cagcggctgg cgatgatatt cgagggggat
     1561 gagctccgcg cgttcaaccg ctgcggggtg ggtggcgaca cggccgcgcg tccctacccg
     1621 tacgcgcccg gcccgggccg cgcgctgctg aacaacctgc gcggtccctc ccgctcggtc
     1681 gtcatcgccg tggagcaccc gatgttcgag ccggccgccg gctacttcgc ggacatcctg
     1741 tcgcgcgatc gtttccgccc caccgtgacc tccacgctcg ccggagccga ccttcgcctg
     1801 gtcgcggttc cgctcggtga gagcgaggcg gatggtctgc gctgcttgag cggcctgctc
     1861 gccctcgata ctctggccgg ctcggcggcg gcgcagtcgc tgcaggctgt cagcagttgc
     1921 ctcgattcgg cggccctggc ggcggagccg gcggcggcgc tgcggtacgt gcaactggcc
     1981 gagagcgtcc tgcgcgacga cctcggggta ttcccgctgt ttcgccccac cctgtatttc
     2041 acggcccgcc ggagcctgct cggcgaggtg ttcgaccgcc ggggcggtat cgacgtatcc
     2101 ggtctggccc gcgtccgaat tccgcagaac cggaaggact gccggccatg actttcacgg
     2161 gccgcatccg cttgtacctg atcctcgtgg ccgtggtgcc gacggtcctg gtggtgtcgg
     2221 tcatctacct gctcgggatg cgccaggctg cggcggccga gcggcggtcg gcgcagcaca
     2281 gccttgaccg gttcgcgcag taccagcggg cggtggccga tgaattgcgg caggcggtga
     2341 tcgccgccgc cgccacgccc tcggccgcgc gcgccctgct tctgctcaaa gccggccggg
     2401 catcggatgt ccgtttcgac gccgtaccgg cggggctgga cttcttcgaa attgccgatt
     2461 cctccctgct cgtgcaggcc agcgcccgcc gcccggggct catcggggag aggctcccgt
     2521 ccctggacag cctggcggcg gactcggcgc cgctggcgtc cgtggagtat gacctgaacg
     2581 gcccccacgc cgcgctggcg tggagacttc cggccgaaag cgcctatgtc gtctatgccg
     2641 gccgctatct cgacagccag tacaccggga cggtggcgcg cctgatcgga gccgacgtgc
     2701 gcattgtgct ggccggcgac accggcggcg ggttgtcggg catggacccc ggccgtctct
     2761 acgccgaacg ggattcgctg gtcgcggtgc tcgccggcgg cgatgcagcc ggctggttcc
     2821 tcgccgcttc cttccggtcc ggccccgagc agcgcgtttt tggcaacctc ctggttgtga
     2881 gcgggctggt cgcgctcgga tcggtgctgg cggccgtggc gctcggcctg tatatcagcg
     2941 ggcgcgccca gcgggaattc gacaacctcc tcacggcgac cgcccgcgtc tcgggcggtg
     3001 acctcagcac gcccgtcatg gcctacgagg aaggggagtt cgcccaactg gcggattctt
     3061 tcacggccat gaccttccgc ctccgagacg cccagcggcg gctggccacg actgagaaga
     3121 tcgcggcctg gcaggccgtc gaccgcaaga tcgcccatga ggtcaagaac cccctcaccc
     3181 cgatcgtgat cagcaccgac gatctccgcc gcgatgtcga caagcggatc cgctcggtca
     3241 ttcgnnnnnn nnnngttcca ggaacagctc ccggatttcc ccaggatcct cgaagagacg
     3301 acggcgacga tcaagtccga ggtggagcgc ctcacccgcc tgctcgatga attcgtgggc
     3361 ttcgcacgga tgggaccgcc ggtcatccgg gatgtggccc cggcgagact cctggaaccg
     3421 ctggccgcgc tctaccgcgg cgagacgggc actggccgtc tgacgttgtc gaacaggagc
     3481 gcgcggcgga gcgtccgcct cgacccggag atgatgcacc aggtgctggt caacctggtg
     3541 aaga
//
LOCUS       DAOOKS010000121         3540 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_32002_length_3540_cov_3.364132, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000121 DAOOKS010000000
VERSION     DAOOKS010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32002_length_3540_cov_3.364132"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1228
                     /locus_tag="PK112_02825"
     CDS             <1..1228
                     /locus_tag="PK112_02825"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HPC11014.1"
                     /translation="DLETIGLGGAGTAAFLVGLGALVEGGFEVRQYLLPDYDDEAALL
                     AAVAEEFSDRTVLVSYNGAAFDLPILLDRFTINRVGREIPRARHVDLLHAVRRLFRRR
                     LRQCDLGNIERDVFGFRRFDDIPGYLVPSVYFDWLSDHKLDEMEKVLQHNRHDIVSLY
                     FLAAVLAEVFESRGERLSEIDDLHSLARVFNRRRDFDLVVDLNRRVESLAGTPDPLAD
                     HPDPRADRAQPGGHPGARALPADVLFFHAQTHKRRGEYDEAVVIWKQLASSIPAPPAT
                     GPATGPASPPATGPAPPPATGPATGSSHLSFPRRREPRHTPTGASDLPIRHNLPADQT
                     PSGPGLTSPADAGTLLRVAYRACLELAIHYEHRAKEFALALAYTRRAESLPDLNPSRR
                     RSLQKRRDRLVRKQEA"
     gene            1353..>3540
                     /locus_tag="PK112_02830"
     CDS             1353..>3540
                     /locus_tag="PK112_02830"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HPC11015.1"
                     /translation="MSDFAEDDVTRSIAPLAEGQIVGHFRIESRIGAGGMGTVFLAHD
                     MDLDRKVALKHLSPELAQDEQFQARFRREARILANLNHPNIVGIYEVFQAGNRSFIAM
                     EYVDGRPLSAQPDRPLAEICQTAVEIASGLKTAHDAGIVHRDIKPANILVGQDGRVKI
                     LDFGLAKRTDDQDITRTGGTAGTFAYMSPEQVRGTAVDRRSDIFSFGVVLYEMVLGRR
                     PFRGEYAAEIAHRIVAEPPSALDPAGTGIPPELLRIIGRCLEKDPVDRYQETGQVVDD
                     LKHFQRVLSGSGDPFAVISSPRSGVGAAPVADALDASQIPARPPQPKPLFLLFGLGLA
                     AVGVALLLAFTPQPLSELERATTAPAQVEKAALDLARRDHSGLLGFRSYTVSDADAEL
                     QEIREFLRPSDREWEALSQWRPAQFYRTAAVSADQQERYDVLTTLDGRCFSFSHSLRP
                     DRRSDSASVDSLRSAAGQYAQAFLGRDLDSLREMPVAVSFSAGVAQTSFQWVEPDTLL
                     AGAVGEVRATFSGAALTDIRSTAAFPEVLRRTIASRGPDWISTGTFCAVTLAFLAACI
                     FVTKKRMWQFPSPSVLVAAYALMVIGLLGGTGAYEKFADTGTAGVLTHVLLLLVLFAF
                     LAVAVYLVIAAVYGVVGRVKPGLLAGLAPLLKGRMERPALAAAASTGFVCGAALYVVC
                     MLYRHSAAVLFDTAGSLSSSSEYVNSGVQVLSLTILGLPFTCAAVGL"
BASE COUNT          521 a         1295 c         1050 g          674 t
ORIGIN      
        1 cgacctcgag accatcggcc tcggcggcgc cggcacagcc gcttttctcg tcggcctcgg
       61 cgctctcgtc gagggcggct tcgaggtccg ccagtatctg ctgcccgact acgatgacga
      121 ggccgcgctg ctggccgcgg tcgccgagga attctccgac cgcaccgtcc tggtctccta
      181 caacggcgcc gcgttcgacc tccccatcct cctcgaccgc ttcaccatca accgcgtcgg
      241 ccgggagatc ccccgtgccc gccacgtcga tttgctccac gcggtccggc gcctgttccg
      301 gcggcgcctg cgccagtgcg acctcggcaa tattgagcgc gatgtcttcg ggttccgccg
      361 tttcgacgac atccccggct acctcgtccc ctccgtctat ttcgactggc tgagcgatca
      421 caaactcgac gagatggaga aagtcctcca gcacaaccgc cacgacatcg tctcgctgta
      481 cttcctcgcc gccgtcctcg ccgaggtgtt cgagtcgcga ggggagaggc tctcggagat
      541 cgacgatctc cactcgctcg cccgcgtgtt caaccgccgc cgcgatttcg atctggtcgt
      601 cgatctcaac cgccgggtgg agagtctcgc cggcactccc gaccccctgg ccgatcatcc
      661 ggaccctcgc gccgaccgcg cccaaccggg cggtcacccc ggcgcccgtg cgctccccgc
      721 cgatgtcctc ttcttccacg cgcagactca taagcgccgc ggggagtatg acgaggctgt
      781 cgtgatctgg aagcagctcg cgtcgtcaat cccggcccct cccgcaaccg gtcccgctac
      841 cggtcccgcg tctcctcccg ccaccggtcc cgcgcctcct cccgccaccg gtcccgcgac
      901 cggctcttct catttgtcat tcccgcgaag gcgggaaccc aggcacaccc cgaccggagc
      961 ttcggacttg ccgatcaggc acaacctccc cgccgatcag accccctccg gtcccggcct
     1021 gacctcaccc gccgatgccg gaaccctcct ccgcgtcgcc taccgcgcct gcctcgaact
     1081 cgctatccac tatgagcacc gcgccaagga attcgccctc gccctcgcct atacccgccg
     1141 cgccgaatcc ctccccgacc tgaatccctc gcgtcggagg agtctgcaga agcgccggga
     1201 tcgcctggtg cgaaagcagg aggcgtgagc cggcggcccc gctgttctga ttgcacttga
     1261 cgcgcttggg caaaagcgac aaattgagcg ctgttggaat gcacgatcgc taatcgcggg
     1321 gcgtcggctc cattattgcg ccaggaggat gtatgagcga cttcgccgag gatgatgtca
     1381 ccagaagcat agcgccgctc gccgagggcc agattgtcgg ccacttccgc atcgagtccc
     1441 gcatcggcgc cggcggcatg ggaaccgtgt tccttgccca cgacatggat ctcgaccgca
     1501 aagtcgctct caagcatctg tccccggagc ttgcccagga cgagcagttc caggcgcgct
     1561 tccggcggga ggccagaatc ctcgccaacc tgaaccatcc gaacatcgtc ggcatctacg
     1621 aggtcttcca ggcgggcaac cgctcgttta tcgccatgga atacgttgac ggccgtccct
     1681 tgagcgccca gcccgaccgg ccgcttgctg agatttgcca gacggccgtt gagatcgcct
     1741 ccgggctcaa gaccgcccac gacgcgggta ttgtgcaccg cgacatcaaa ccggccaaca
     1801 ttctggtagg ccaggacggc cgggtaaaga tcctcgattt cggtctggcc aagcgtactg
     1861 acgatcagga catcacccgc accgggggga ccgcgggcac cttcgcctac atgtctcccg
     1921 aacaggtgcg gggaacggcc gtcgacagac gttcggacat cttctcgttc ggagtcgtgc
     1981 tgtacgagat ggtcctcggc cgccgcccgt ttcgcggcga gtacgccgcg gaaatcgcgc
     2041 acagaattgt cgccgaaccg ccgtcggccc tcgatcccgc cgggaccggc atccctccgg
     2101 aactgctccg cattatcggc cggtgcctgg agaaggaccc ggtggatcgg tatcaggaaa
     2161 ccggccaggt ggtcgacgac ctgaagcact tccagcgcgt cctttccggg tccggcgacc
     2221 cctttgccgt catctcctcg ccccggagcg gagtcggcgc cgcgccggtc gcggatgctc
     2281 tcgacgcgtc gcagattccc gcgcgcccgc ctcagccgaa accgctcttt ctgcttttcg
     2341 gcctgggcct cgccgcagtc ggtgtggcgc ttctgcttgc cttcaccccc cagccgctat
     2401 ccgaactcga gcgggctacg accgcacctg cgcaggtgga aaaggccgcc cttgacctgg
     2461 cgcggcgtga ccacagcggc cttttgggtt tccgatccta caccgtcagc gatgccgacg
     2521 ccgagcttca ggaaatcagg gagttccttc gtccctccga ccgcgagtgg gaggcgctca
     2581 gccaatggcg gcctgcccag ttttaccgta ccgctgccgt gagcgccgat cagcaggaac
     2641 ggtacgacgt gctcacgacc ctcgatggcc ggtgtttttc cttcagccat tcgctgcgcc
     2701 ccgaccggag gtccgattcc gcgtccgttg attcgctccg gagcgcggcc gggcagtatg
     2761 cgcaggcatt tctcggccgg gacctcgatt ccctgagaga aatgccggtg gccgtttcct
     2821 tctccgcagg cgtcgcccaa acgagctttc agtgggtcga gcccgatact ctcctggccg
     2881 gggccgtggg agaagtccgt gcgacgttct ccggcgccgc cctcacggac attcgctcga
     2941 ctgcggcgtt ccccgaggtt ctgcgtcgca ccatcgccag ccggggcccg gactggatct
     3001 ctaccggcac tttctgcgcg gtcacgctgg cctttttggc tgcctgcata ttcgtcacca
     3061 aaaagaggat gtggcagttc ccgtccccgt cggtcctcgt cgccgcttac gccctcatgg
     3121 taatcggcct gctgggcggc accggggcgt acgagaaatt cgctgatacc ggcacggccg
     3181 gcgtgctgac ccatgtgctc ctccttctcg tcctctttgc cttcttggcc gtcgccgtgt
     3241 atctggtcat cgccgccgtt tatggtgtgg tcgggcgtgt caaaccgggc ctcctggccg
     3301 gactcgcgcc cctcctgaag gggaggatgg agcgccctgc tcttgccgcc gccgcgagta
     3361 ccgggtttgt ctgcggcgcc gcgctgtatg tggtgtgcat gctctaccgc cattcggccg
     3421 cggtcctgtt cgatacggcc ggcagcctgt cgtcgtcttc cgagtatgtg aattccggcg
     3481 tgcaggtgct gtctctgacc atcctggggc tgcctttcac atgcgcggcg gtcggcctgt
//
LOCUS       DAOOKS010000122         3534 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_32075_length_3534_cov_3.287151, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000122 DAOOKS010000000
VERSION     DAOOKS010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3534
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32075_length_3534_cov_3.287151"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /gene="obgE"
                     /locus_tag="PK112_02835"
     CDS             complement(<1..656)
                     /gene="obgE"
                     /locus_tag="PK112_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HPC11016.1"
                     /translation="MFVDYAEIEVTAGKGGHGVVSFHREKFIPKGGPDGGDGGHGGSV
                     AAVADPQLTTLLDYRYKKHYRAENGQPGQGSLKTGRSGHDIELRLPVGTIVTDLDTGE
                     QLADLDSPGARVILARGGKGGLGNNHFKTSTNQTPRKATTGYPGEHRRLALELKLLAD
                     VGLVGKPNAGKSTILATFSAARPKIADYPFTTLIPNLGIVRLREHKSCVMADIPGLIE
                     G"
     gene            789..3164
                     /locus_tag="PK112_02840"
     CDS             789..3164
                     /locus_tag="PK112_02840"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HPC11017.1"
                     /translation="MKYSIVCGLFPALLAGAAAASVIHVPGDHATIQAGIAAAAAGDT
                     VQVAPGTYLENISLAGIDIVLLGAGMDSTILKPADGGLPLVRIHQGETRAAVVRGFSF
                     EESGMSSALVIEGSSPTICFNRITRCPNLSNTPAVLSADSASAPAIHHNLFLRNDGYY
                     QIKAAGPIWIYNNTIYDSRYIGISASHDSAAILNNIIIDCQHRALITSLARIDYNLVL
                     YDIYCCLPYAEHDLLADPLWCDSAAGDLRLWIGSPLIDAGHPDYRDPDGTPTDLGAFS
                     VAGAPPAAWQIAVDSGTLVPTIAWQYADTSGLTQTGYEIEIGRDRDWTAAELWASGPV
                     PSGIPAAAYGGGPLADRGLYYYRLRLQNAEGWGSWREGLFAVQIAPHRKIAVPADLPT
                     IQEAIFAAADGDTIAVAAGTYTGPFNRDLNFLGKQVVLYAPSGPDSTILNLGGDAGLQ
                     RHAFTFRNDEGPTTEVVGFTITNGLGFIRPYSQSGVIKCEGTGPTFRNCRIVDNAGSG
                     IMFSAELGDTLRVIGCDISRNSWEGIGGGTSGVIAVDSTSFLDNSADGIQVGWGTVLR
                     ADRSLFVGNAQRGIFAWYEMVPRGLYVTNCTFADNGTGLWYDWDFPKDGAPAPAVLDS
                     GLSRIIGNVFAFNHGDGLHGSAPCPYFVTCNDWFGNGAADVVIFTDDPIDSANNNSVD
                     PLFCDTAAGDYRVAAGSVCLPENNACGAVIGARGLGCEGGCCAHMGDFQHNGAVTIAD
                     LTAFVAHLFRGGPAPLCFEEVDFNRDAQLTVVDLTLLVAAMFRGAPWPVCP"
     gene            complement(3202..>3534)
                     /locus_tag="PK112_02845"
     CDS             complement(3202..>3534)
                     /locus_tag="PK112_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11018.1"
                     /translation="VIAYPNPYRADGGYRGNGFEGRLAPDRPEDRVRAVHFANLPARC
                     TIRILTLDGDLVREIVHDQPPGSARANHDQWDLITRNTQTVVSGLYYWTVEDGQGNVQ
                     LGKLAIIM"
BASE COUNT          562 a         1227 c         1108 g          637 t
ORIGIN      
        1 ccctcgatga gtccggggat gtcggccata acgcaggact tgtgctcgcg cagtcggacg
       61 atccccagat tcgggatcag cgtcgtgaac gggtagtcgg cgatcttggg gcgggcggcc
      121 gagaacgtgg ccaggatagt cgatttcccc gcgttcggct tgcccaccag gccgacgtcg
      181 gccagcaact tcaactccag cgccagccgg cggtgctcgc cgggataccc cgtcgtcgcc
      241 ttgcgcggcg tctgattggt cgaggtcttg aagtgattgt tgccgagtcc ccctttgccc
      301 ccgcgggcga ggatcactct cgcccccggc gagtcgagat cggcaagctg ctcgccggtg
      361 tcgaggtcgg tgacgatcgt ccccaccggg agccgcaatt cgatatcgtg ccccgaacgc
      421 ccggtcttga gcgacccctg gccgggctgt ccgttctccg cccggtagtg cttcttgtac
      481 cggtaatcga gcagggtagt cagttggggg tcggccaccg cggcgacact cccgccgtgg
      541 ccgccgtccc cgccgtccgg accccctttc gggatgaact tctcccggtg gaaggagact
      601 actccgtgtc cgcctttgcc ggcggtgacc tcgatctcgg cgtagtcgac aaacatgggt
      661 gacaatatgg gccccgccgc cgacaatgtc aaaccacacg tgtattgaca gaacccccga
      721 tccgcgctat cttgggagaa agcaggaacg cgttcatcgg tatcgcgctc atcccaaaga
      781 aggctgccat gaaatactca attgtttgcg gtctgttccc ggcgctcctc gcgggtgccg
      841 cggccgcgtc cgtaatccat gttcccggcg accacgcaac catccaggcg ggcatcgccg
      901 ccgccgcggc cggcgacacc gtgcaggtgg cgcccggaac ctatctggaa aacatcagcc
      961 tcgccgggat cgacatcgtc cttctcggag cgggcatgga ttccacgatc ctgaaacccg
     1021 ccgacggcgg cctcccgctt gtccgcatcc accagggaga gacccgcgcc gccgtggtgc
     1081 gggggttcag tttcgaagag agcgggatgt cgagcgccct ggtgatcgag gggagttccc
     1141 ccacgatctg tttcaaccgg atcacccgct gcccgaatct cagcaacacg ccggcggttc
     1201 tctcggccga ttcggcgagc gcccccgcga tccatcacaa cttgtttctt cgcaacgacg
     1261 gttattatca gatcaaggcg gcggggccga tctggatcta caacaacacc atttacgact
     1321 cccgttatat agggatatcg gcctcgcacg acagcgccgc catcctcaac aacatcatca
     1381 tcgactgcca gcaccgggcg ctcatcacct cgctcgcccg gatcgactac aacctcgtcc
     1441 tctacgacat atactgctgt ctcccgtacg ccgaacacga tctgctcgcg gacccgctgt
     1501 ggtgcgattc ggctgcgggc gacctgcgcc tgtggatcgg ttcgccgctg attgacgccg
     1561 ggcatccgga ttaccgggat ccggacggca cgccgaccga tctcggcgct ttctcagtgg
     1621 ccggtgcgcc gcccgccgcc tggcagatcg ccgtcgactc cgggactctc gttccgacca
     1681 tcgcctggca gtacgcagat acgagcggac tgacgcagac cgggtacgaa atcgagatcg
     1741 gccgcgaccg cgactggacg gcggccgagc tgtgggcatc cgggccggta ccgtccggca
     1801 tcccggcagc cgcgtacggg ggcgggccgc tggccgaccg ggggttgtac tactaccgcc
     1861 tccgcctgca gaacgccgag ggctggggtt cctggcggga agggcttttc gcggtgcaga
     1921 tcgcccccca tcggaaaatc gccgtgccgg ccgaccttcc cacgattcag gaagccattt
     1981 tcgccgccgc cgacggcgac acgatcgccg tggccgccgg cacgtacacc ggcccgttca
     2041 accgcgacct caacttcctc ggcaaacagg tcgtcctcta tgccccgtcc ggcccggact
     2101 ccacaatcct taatctcggg ggcgacgccg gcctccagcg ccacgcgttc actttccgca
     2161 acgacgaggg gccgacgacg gaggtggtcg ggttcaccat caccaacggt ctcggcttca
     2221 tccgccccta ttcccaatcg ggcgtcatca agtgcgaagg cacggggccg actttccgca
     2281 actgccgcat tgtcgacaac gcgggctcgg gcatcatgtt ctcagccgag ctgggagaca
     2341 ctttgcgggt gatcggctgc gacatcagcc gcaactcctg ggaggggatc ggcggcggga
     2401 cgagcggcgt gatagcggtc gactcgacga gtttcttgga caacagcgcg gacggcatac
     2461 aggttggctg ggggacggtg ctccgggccg atcgctcgct ttttgtcggc aacgcccagc
     2521 gcggaatttt cgcctggtac gaaatggtgc cgcgcggtct ctatgtcacc aactgcactt
     2581 tcgcggataa tgggaccggg ctctggtacg actgggattt tccgaaggac ggcgctccgg
     2641 ccccggccgt gctggactcg ggcctgtcgc ggataatcgg caacgtcttc gccttcaacc
     2701 acggcgacgg tctgcacggc tccgcgccct gcccgtactt tgtcacctgc aacgactggt
     2761 tcggcaacgg cgcggcggac gtggtgatct tcaccgacga ccccatcgac agcgccaaca
     2821 acaactcggt cgacccgctc ttctgcgaca cggccgccgg cgactaccgg gtggcggccg
     2881 gctccgtctg cctgccggag aacaacgcct gcggggcggt catcggcgcc cgcggcctcg
     2941 gctgcgaagg cggctgctgc gcgcacatgg gcgatttcca gcacaacggc gccgtcacta
     3001 tcgccgacct gaccgctttc gtcgcccatc tcttccgcgg cggtcccgcc ccgttgtgtt
     3061 ttgaggaggt cgatttcaac cgcgacgcgc aactcactgt cgtcgacctc accctgctgg
     3121 tggcggcgat gtttcgcggc gccccctggc ccgtgtgccc gtgaccgggc gtggcggtgg
     3181 tccgacgcgg gccgcccggc attacatgat gatcgccagc ttgccgagct gcacgttgcc
     3241 ctgcccgtct tccaccgtcc agtagtacag cccgctgacc accgtctgcg tgttgcgggt
     3301 gatgagatcc cactggtcgt gattggcgcg cgccgatccc ggcggctgat cgtggacgat
     3361 ttcccggaca aggtcgccgt cgagcgtcaa gatcctgatc gtgcagcggg cggggagatt
     3421 ggcgaaatgc acggcccgca cccggtcctc cggccggtcc ggggccagcc ggccctcgaa
     3481 cccgttcccc cggtacccgc cgtcggcccg gtagggattc ggataggcga tcac
//
LOCUS       DAOOKS010000123         3525 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_32199_length_3525_cov_3.909510, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000123 DAOOKS010000000
VERSION     DAOOKS010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32199_length_3525_cov_3.909510"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            175..846
                     /locus_tag="PK112_02850"
     CDS             175..846
                     /locus_tag="PK112_02850"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MtnX-like HAD-IB family phosphatase"
                     /protein_id="HPC11019.1"
                     /translation="MTRPAVFCDFDGTIAVRDVGYSIFHRFSGGRNLALLPDWKAGRM
                     SSREILRREAAMAPVTESELLEFLEDFTLDPGFAAFADRCHRQKIDLVVVSDGLDLYI
                     NLLLARNRLSHLPLIANHGYVRDGHIEIEFPFDNRDCARCGSCKGERIREYRLSHPGA
                     GPAVFVGDGFSDACAAHEADLLFAKKDLERYCRMHNIPFVPYQDFFDVARELERRELL
                     SRNDV"
     gene            881..3367
                     /locus_tag="PK112_02855"
     CDS             881..3367
                     /locus_tag="PK112_02855"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HPC11020.1"
                     /translation="MAGGFGTRLRPLTINIPKPMAPIGNLPMMEHVVSLLVRHGITDV
                     TALLYFQPEKIRNHFKNGAAFGVNMEYAQPDDDYGTAGAVRYALTTATEPVLVISGDL
                     ITDFDLTEAIAWHAARRSQATLLLTRMENPLAYGIVITDDDGRIVRFLEKPSWGEAFS
                     DTINTGIYILEPAAIEMIPPRVNFDFSQNLFPLMLSRQMGLFGKIMAGYWKDVGNVGE
                     YRRVHADFFNGTLNLDLKRPVQPLPSGELYAGENVRIADNVQLSGRVVLGHDAKIEAG
                     ARLHNCVIGDRTRIGPGAKLSNTVVWHDANIGAESLVAESVICNSARLGRNVQFMDDV
                     IVADDTVIGDRATVKANCKIWPGKTVDSGAIVSTSIVWGDRWNRELFTNSKITGLALT
                     EITPEMTVKVGAAFGAFLGKGAAVLTSRDASDGSRLLKRGLMSGLLAAGVNVADLETQ
                     PIPVVRYGLTRGSYAAGIYVRHSPADFRLIDAIFFDGSGLDMPTAKLKKVERMYFGED
                     FERASLGDIGHLDQPQHVLEDYREDFLKSLDIDLIRKAGFKVVIDHSNGSSSQIFPTL
                     SSELGVSAVELNANLNPRKFSTSPEEMAQAMVQLSAIVSSLKADIGFLLNPAAEKLTV
                     VDELGQPVDPQLLLLIVLDLFLQLNKVRRVAVPVAASMGVEEIARDRGAEVIRVANDH
                     LSMMEVMRRGEADFVGGTRGGFIFPGFQSGADAMFATCKILEMMAATRSRFGALRRQF
                     EDLQRETISVPCPWSKKGTVMRHLIVNSQTRERQLVDGVRIRENGGWVLLIPDRETAS
                     FNIMAETRSREATQALVNRYRSLVEEWQTE"
BASE COUNT          628 a         1189 c         1071 g          637 t
ORIGIN      
        1 actttatttg atcatcgctt tgcaactcgc ggcactcatc gtcgccttca tcatcgtcct
       61 gatggcggag ctgttccggc gcgcccgact ctaccgctac gcgctgctgt cgtacctgct
      121 cctggtgatt ctcctttcga ccattgcgct caagttgtat gttaccggat gaccatgacg
      181 cgaccggccg ttttctgcga cttcgacggc accatcgccg ttcgcgatgt cggctacagc
      241 attttccacc gcttctccgg cggccggaat ctggctctcc tccccgactg gaaagcgggg
      301 cgcatgtcga gccgggagat cctccgccgc gaggccgcga tggcgccggt gaccgagagc
      361 gaactgcttg agtttctcga ggatttcaca ctcgaccccg gtttcgccgc cttcgccgac
      421 cgctgccatc ggcagaagat cgatctggtg gtcgtgtccg acggcctcga tttgtacatc
      481 aacctgctct tggcccgcaa ccgcctgtcg caccttccgc tgatcgccaa tcacggctat
      541 gtgcgcgacg ggcacatcga aatcgaattc ccctttgaca accgggactg cgccaggtgc
      601 ggcagctgca aaggggagcg gattcgcgag taccgcttaa gccacccggg ggccgggccg
      661 gcggtctttg tcggcgacgg attttccgac gcctgtgcgg cgcacgaggc cgaccttctc
      721 tttgcgaaaa aagaccttga acgctactgc cgcatgcaca atattccatt cgtgccgtat
      781 caggatttct tcgacgttgc tcgcgaactg gagcgacgcg aattgttaag tcgtaacgac
      841 gtttagactt aagggagaag tcgacatgaa ggcgatcgtc atggcgggcg gtttcggaac
      901 ccgactgcgc cctttgacca ttaacatacc caaacccatg gccccgatcg gcaacctgcc
      961 catgatggaa cacgtggtgt ccctcctggt caggcacggc atcaccgacg tgaccgcgct
     1021 gctgtacttc cagccggaga agatccgcaa tcattttaag aacggcgcgg cgttcggggt
     1081 gaacatggag tacgcgcagc cggatgacga ttacggcacg gcgggggcgg tccgctacgc
     1141 tctcaccacg gccacggaac cggtgctcgt gatctcgggc gacctgatca ccgatttcga
     1201 tctcaccgag gctatcgcct ggcacgccgc ccggcgctcg caggcgacgc tcctgctcac
     1261 ccgcatggag aatccactcg cctacggaat cgtgatcacc gacgacgacg gtcggatcgt
     1321 gcgcttcctg gagaaaccct cgtggggcga ggccttctcc gataccatca acaccggcat
     1381 ctacattctc gagcccgcgg ccatcgagat gatcccgccc cgggtcaatt tcgacttctc
     1441 gcagaatctc ttccccctca tgctctcgcg gcagatgggg ctgttcggta agatcatggc
     1501 cggctactgg aaagacgtcg gcaacgtggg ggagtaccgg agagtgcacg ccgacttctt
     1561 caacggcacg ctcaacctcg atctcaagcg gccggtgcaa ccgctcccct cgggcgagct
     1621 ctacgccggg gagaatgtcc gtatcgccga caacgtccag ctctccgggc gtgtcgtcct
     1681 cggccatgat gcgaagatcg aggcgggggc caggctccac aattgcgtca tcggcgaccg
     1741 cacgcggatc ggcccgggcg ccaagctcag caacacggtg gtgtggcacg acgccaacat
     1801 cggcgccgag agcctggtgg ccgagagcgt gatctgcaac tccgcacggc tcggccgcaa
     1861 cgtgcagttc atggacgacg tcatcgtcgc cgacgacacg gtgatcggcg accgcgcgac
     1921 ggtcaaagcc aactgcaaaa tctggccggg caagacggtc gattccggcg ccatcgtctc
     1981 cacctcaatc gtctggggcg accgctggaa ccgggagttg ttcaccaact cgaaaatcac
     2041 cggcctggcg ctgaccgaaa tcacgcccga gatgacggtc aaagtcggcg ccgccttcgg
     2101 cgctttcctc ggcaagggcg cggccgtgtt gaccagccgc gacgcctcgg acgggtcgcg
     2161 tctgctcaaa cgcgggctca tgtcgggcct cctggccgcg ggcgtcaacg ttgccgacct
     2221 cgaaacccag cccattcccg tggtgcgcta cggtctgacg cgggggagtt acgccgccgg
     2281 gatctacgtg cgccactccc cggccgattt ccgcttgatt gacgcgatct tcttcgacgg
     2341 ctccgggctg gacatgccga cggccaagct gaagaaggtg gagcgcatgt atttcggcga
     2401 ggatttcgaa cgcgcctcgc tcggcgacat cggccacctc gaccagcccc agcacgtgct
     2461 cgaggactac cgcgaggatt tcctcaaatc gctcgacatc gacctgatcc gcaaggccgg
     2521 attcaaagtc gtcatcgacc actccaacgg ctcctcgagc cagatcttcc cgaccctgtc
     2581 ctccgagctc ggcgtctcag ccgtcgaact gaacgcgaac ctcaacccgc gcaagttctc
     2641 gacctcgccc gaggagatgg cccaggccat ggtgcagctc tccgccatcg tctcctccct
     2701 caaggccgac atcggcttcc tgctcaaccc ggcggccgag aaactcaccg tggtcgatga
     2761 actcgggcag ccggtcgacc cgcagctgct cctgctcatc gtcctcgatc tgttcctgca
     2821 gttgaacaaa gtgcggcggg tggcggtccc ggtggcggcc tcgatgggcg tcgaggaaat
     2881 cgcccgcgac cgcggcgccg aggtcatccg cgtcgccaac gaccacctct cgatgatgga
     2941 ggtgatgcgg cgcggagagg cggactttgt cggcggcacc cgcggcggct tcatcttccc
     3001 cggcttccag agcggggccg atgcgatgtt cgccacctgc aagatccttg agatgatggc
     3061 tgcgacccgc agccgcttcg gcgccctccg ccgacaattc gaggacctcc agcgcgaaac
     3121 catctccgtc ccctgcccgt ggtcgaagaa gggcacggtt atgcgccacc tgattgtaaa
     3181 ctcccagacg cgcgagcggc agttggtcga cggcgtgcga atcagagaaa acgggggctg
     3241 ggtcctgctc atccccgacc gcgagacggc gtcgttcaac atcatggcgg aaacgcgttc
     3301 gcgcgaggcc acccaggcgc tcgtcaaccg ctaccggagc ctggtggagg agtggcagac
     3361 cgaatagggg gaaacacgcc atcaggcatg tgcggcgccg acccgggcag gagcgcggcc
     3421 tcgcggcgcg gcccggcccg ccgaccgcgg agaccgccgc tgctcagggc gccggcggag
     3481 tgtcgtcgct gccggccgcg cgggtccgcg atggatcctt aatga
//
LOCUS       DAOOKS010000124         3510 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_32377_length_3510_cov_3.173082, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000124 DAOOKS010000000
VERSION     DAOOKS010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32377_length_3510_cov_3.173082"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="PK112_02860"
     CDS             <1..229
                     /locus_tag="PK112_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11021.1"
                     /translation="DIRQALIQLRTTVHALSAEQAEAKAAEGFTLISATIGAGERLVD
                     EYYFRRKGLGLATLCITVLAVALWLKIRRLR"
     gene            532..888
                     /locus_tag="PK112_02865"
     CDS             532..888
                     /locus_tag="PK112_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007506138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HPC11022.1"
                     /translation="MSEYLAILMFIAAGIAIVLITFFVARLVRPHHPYPAKDMNYECA
                     EEPIGGAWIQFNNRFYIFALIFVLFDVEVVFLFPWAVAFGQLGLFALVEMVVFILILF
                     FGLYYAWRKGALKWVS"
     gene            876..1394
                     /locus_tag="PK112_02870"
     CDS             876..1394
                     /locus_tag="PK112_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HPC11023.1"
                     /translation="MGVIRKLPVYLEHIPGGSLVLTNLETVLHQSQAKSLWYLLFGTA
                     CCAIELMCTGASHYDFDRLGMIFRSTPRQADLLIAAGTITKKMAPRLRRLYDQMAEPR
                     YVIAMGGCTIKGGPFYYDCYAVEKGIDHIIPVDIYIPGCPPRPESLLEGCLHLQEKIK
                     KQRLLDWQEKPA"
     gene            1391..1882
                     /locus_tag="PK112_02875"
     CDS             1391..1882
                     /locus_tag="PK112_02875"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HPC11024.1"
                     /translation="MTREELGNYVAGKFGDRVTRLATGRYDPLFLVNRASDLLEVARA
                     LRDDEQLRMDYLCNAGAIDTGTAAAPEKRFEIHYALASIRHSCRLDLKITLPAEKPEV
                     DSLQAVWPAADWFEREFWELYGIHVRNHGKLGQFLLPDNWDQGFPMRKDWDAPDFIRM
                     PEK"
     gene            1886..2995
                     /locus_tag="PK112_02880"
     CDS             1886..2995
                     /locus_tag="PK112_02880"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012882083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HPC11025.1"
                     /translation="MANALRTEEFIVNMGPVHPSTHGVCRLLLTMDGEKVMKVEPVIG
                     YLHRSIEKICENRTFAQCVPLMDRVEYVTAMSCGYMFAGAVEKLGGIEVPPRAQYLRT
                     IMLELNRIASHLLFYGVTAMDIGAYTPFLYGLRERELIIDLFEETCGQRLTYNYIRIG
                     GVSQDIRPDFADGTRKALKIISAKMSDYTGLLNENPIWLRRTKGVGVLPADLAIAYGV
                     SGPNLRASGVNYDIRRNDPYLIYDRLDWNVATRPNGDCFDRYMLRLDEIRQSIRIVNQ
                     ALDGLPEGEIMAKGVTVNYKPPAGEVYHRVENSRGELGAYIQADGGKKPVRVKLRGGS
                     YNALMALPHFATNVLIADLVAIFATLDVIMPEVDR"
     gene            2992..>3510
                     /locus_tag="PK112_02885"
     CDS             2992..>3510
                     /locus_tag="PK112_02885"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HPC11026.1"
                     /translation="MILPALLALEMFELYRVFFWLGERLHDLGLPGWMIDPVRYGTLG
                     AMMFGVLCLVALFLVWWERKIAGHTQQRFGPMRHGWHGWYQTVMDALKLLQKEDIRVA
                     TRDRAVFFWAPVVGFVAAFLAYIVIPWGDNLIVADLNIGILYIMAVTTFTVISLLMAG
                     WGSNNKYALLGGM"
BASE COUNT          643 a         1036 c         1158 g          673 t
ORIGIN      
        1 ggatatccgg caggcgctca ttcaactgcg gacaaccgtg cacgcgctca gcgccgaaca
       61 ggcggaggcc aaagccgccg aggggttcac cctcatcagc gccaccatcg gggccggcga
      121 acggctggtc gacgagtact acttccggcg caagggactg ggtttggcga cgctgtgcat
      181 tacggtgttg gcggtcgcgc tgtggctgaa gatacggcgg ctcaggtagc gagcgcgcgg
      241 ccgggcgaat ccggatgaga gttcgttatt ttatacagaa tcgtatcgtt ttcgggttaa
      301 tcgaccgttc gagtcctctt ttctaagtcg gtttcggcga acactataga gccgccgaac
      361 ggttctcgtt tcctattgtg gcggaacagg tttggtggtg caaatggccc caaaaatcgc
      421 ttgacacggg gggaaaaaga gatttgttac gctctcgaac ttttgtgaat tcagtcacat
      481 gcgccgactg acgcaaatgt tggagagggc agctaaaaaa ggtcaaggaa tatgtcggag
      541 tacctcgcca ttctgatgtt catcgccgcc gggatcgcca ttgtcctgat caccttcttc
      601 gtggcccggc tcgttcgccc gcaccacccg tacccggcca aagacatgaa ctacgagtgc
      661 gcggaggaac cgatcggcgg ggcctggatt cagttcaata accgcttcta tattttcgcc
      721 ctcattttcg tgctgttcga tgtggaagta gtgttcctct ttccgtgggc ggtggcgttc
      781 gggcaactcg ggctgtttgc actggtggag atggtggtgt ttatcctcat cctgtttttc
      841 ggtctctact atgcctggcg gaagggagcg ctcaaatggg tgtcataagg aaactccccg
      901 tctaccttga gcacatcccc ggcggatcgc tggtgctgac gaacctggag acggtgctgc
      961 accagagcca ggcgaagtcg ctgtggtacc tgctgttcgg gaccgcctgc tgcgcgatcg
     1021 agctcatgtg caccggggcg tcgcactacg attttgaccg cctggggatg atcttccggt
     1081 cgacgccgcg ccaggccgac ctcctcatcg ccgccggcac catcaccaag aaaatggccc
     1141 cgcggctccg gcggctgtac gatcagatgg ccgagccgcg ctacgtcatc gccatgggcg
     1201 ggtgcacgat caagggcggg ccgttctact acgactgcta cgcggtcgaa aaaggaatcg
     1261 accacatcat ccccgtggac atctacatcc ccggctgccc gccgcggccg gaatcgctgc
     1321 tcgaggggtg cctgcacctc caggagaaaa tcaagaagca gcggctcctc gattggcagg
     1381 agaagccggc gtgacgagag aagaactcgg caactacgtg gccggtaagt tcggcgaccg
     1441 ggtcacgcgt ctggcgaccg ggcggtacga tccgctgttc ctggtgaacc gggcttcgga
     1501 tctgctcgag gtggcgcggg cgctgcggga tgacgagcag ctgcggatgg actacctgtg
     1561 caacgccggg gccatcgaca ccggcaccgc cgcggccccg gagaaacgct tcgagatcca
     1621 ctacgcgctg gcctcgatcc gccacagctg ccgcctcgat ctgaaaatca ccctgccggc
     1681 cgagaagccg gaggtggact cgctgcaggc ggtctggccc gcggcggact ggttcgagcg
     1741 ggagttctgg gagctgtacg ggattcatgt gcgcaaccac ggcaagctgg gccagtttct
     1801 gctgccagac aactgggacc agggattccc gatgcgcaag gactgggatg cgccggactt
     1861 tatccggatg ccggagaagt gagcgatggc caacgcgctg cggacagaag agttcatcgt
     1921 caacatgggc ccggtccatc cctcgacgca cggcgtctgc cggctgctgt tgacgatgga
     1981 cggcgagaaa gtgatgaagg tcgagccggt gatcggctac ctgcaccggt cgatcgaaaa
     2041 gatatgcgag aaccggacgt tcgcccagtg cgtgccgctc atggaccggg tggagtacgt
     2101 cacggccatg tcctgcgggt acatgtttgc gggggcggtc gagaaactcg gggggatcga
     2161 ggtcccgccc cgggcgcagt accttcggac gatcatgctc gagctcaatc gtatcgcctc
     2221 gcacctgctg ttttacgggg tgacggcgat ggatatcggg gcctacaccc ccttcctcta
     2281 cggactgcgc gaacgggagc tgattatcga tctgttcgag gagacctgcg ggcagcggct
     2341 gacctacaat tacatccgga tcggcggggt gtcgcaggac atcaggccgg attttgccga
     2401 cgggacgcgc aaggcgctga agataatcag cgccaagatg tccgactaca ccgggctgct
     2461 gaacgagaat ccgatctggc tgcgccgcac gaagggggtc ggggtgctgc cggcggacct
     2521 ggcaatcgcc tacggagttt cgggaccgaa cctgcgggcc tcgggagtga actacgatat
     2581 tcgccggaat gatccctacc tcatatacga ccggctggac tggaatgtcg ccacgcggcc
     2641 aaacggggac tgcttcgacc gttacatgct gcggctcgac gagatccggc agtcgatccg
     2701 gatcgtgaac caggcgctcg acggactgcc ggagggggag atcatggcga agggggtcac
     2761 ggttaactac aagccgccgg cgggcgaagt gtaccaccgg gtggagaatt cgcgcggcga
     2821 gctgggggcg tacattcagg cggacggcgg gaagaagccg gtgcgggtga agctgcgcgg
     2881 gggatcctat aacgccctca tggcgctgcc gcatttcgcc accaacgtgc tcatcgccga
     2941 cctggtggcg attttcgcca ccctcgatgt catcatgccg gaggtcgacc gatgattctg
     3001 ccggccctgc tcgcgctgga gatgttcgag ctgtaccggg tctttttctg gctgggcgag
     3061 cgcctgcacg acctcggcct cccggggtgg atgatcgacc cggtccgcta cgggacgctc
     3121 ggggcgatga tgttcggggt gctctgcctg gtggccctct tcctcgtctg gtgggagcgc
     3181 aagatcgccg gccacaccca gcagcgcttc ggcccgatgc gccacggctg gcacggctgg
     3241 taccagacgg tcatggatgc gctgaaactg ctgcagaaag aggatatccg ggtggcgacg
     3301 cgcgaccggg cggtgttctt ctgggcgccg gtggtcggct tcgtggccgc cttcctcgcc
     3361 tacatcgtga tcccctgggg agacaacctc atcgtcgccg acctcaacat cggcatcctc
     3421 tacatcatgg cggtgacgac cttcacggtg atctcgctcc tgatggccgg gtgggggtcc
     3481 aacaacaagt acgccctcct cggcgggatg
//
LOCUS       DAOOKS010000125         3491 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_32619_length_3491_cov_2.932771, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000125 DAOOKS010000000
VERSION     DAOOKS010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3491
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32619_length_3491_cov_2.932771"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1191)
                     /locus_tag="PK112_02890"
     CDS             complement(<1..1191)
                     /locus_tag="PK112_02890"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HPC11027.1"
                     /translation="MIAGCSDDETTGPGGGGGGAWTLVSPTTEGNLYRGACFVDATTG
                     WLVGVDGRIVKTTDGGTTWIEQASQTNRTLRAVDFVSRQDGWVVGDDGRVRRTEDGGT
                     TWSLPLLAVTDRSLSAVEGVTARRVFAVGEEGILYYSLDAGRRFSAVPLEDAGPLRDI
                     VVFDTSAVAVGDDGFIARFFNYDWEIERLTADTTVDTLPVDPDTLPDDFPADTVIDTT
                     FVPGDTIVDTTYWWPDTLVDTTYFTVDTIWSGFETVTAEVDDNLLGVAYAGGNSLWAV
                     GENGVFLSSDDRGASWEVVDLGVTAHLTSVASDGAGGGIITGLNGTVLVTSDNGATWA
                     AAQSGYALDLYASSSPAADNFWASGDAAVFRSADAGETWEQRVTGTSLLPTLTDIVFV
                     SPDSG"
     gene            complement(1449..1658)
                     /locus_tag="PK112_02895"
     CDS             complement(1449..1658)
                     /locus_tag="PK112_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11028.1"
                     /translation="MRDEAQRGKHTSSRMKRKGRPGQRPARHFRGGSDEAGRRPDRRR
                     QLEQLAETEAEEMLRQDESDEFSPS"
     gene            1998..2618
                     /locus_tag="PK112_02900"
     CDS             1998..2618
                     /locus_tag="PK112_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11029.1"
                     /translation="MLDNRYRRGFSQERLAAIQEPRVRKDSRGYFLMSLSENTKVYFE
                     DFYRFLERTYAKARAERSRLDERLFTTAPEQSELIAYYRARGVIVDLLMQTIIRFYTD
                     GGNFGVVMSPWCFGTVVLEKLEVYRERLSRGEVEDPNIPDYPYYTIRYLDEIHKAALL
                     ELFDFPEKAFQMRWQYSELLKRYSQILSDITTSLNSVLGTIKNYGS"
     gene            2784..>3491
                     /locus_tag="PK112_02905"
     CDS             2784..>3491
                     /locus_tag="PK112_02905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPC11030.1"
                     /translation="MASGTAKITVTPQYLPILLDSLRVDSVLDFDLYLTVNGNMVLYR
                     SADLPFTDRTRRTLLDNRVDRLFITDDNRSRYQRYIERNLPKIMTDPGIREEKKANIL
                     YDTSTNLVKEVLANPTYGENIQRTKDLVTNTVEFILQGKEAFHTLLKITSFDYYTYTH
                     SVNVCTFSLALAQQLGYGDETFLNELGVGALLHDVGKSRVSERILNKRAPLNPTEFEI
                     MKRHPQWGAEILEETDQI"
BASE COUNT          696 a         1142 c         1044 g          609 t
ORIGIN      
        1 accggagtcg gggctgacaa aaacaatatc cgtcagcgtc ggcagcaagc tcgtcccggt
       61 cacgcgctgc tcccaggtct ccccggcatc agcggaccga aagacggcgg catcgccgga
      121 ggcccagaag ttgtcggccg ccggagacga cgaagcatag agatcgaggg cataccccga
      181 ttgcgccgcc gcccatgtcg cgccgttgtc gctcgtgacc aggacggtgc cgttgagacc
      241 ggtgatgatt ccgccgccgg cgccgtcgct ggcaaccgac gtgaggtgcg cggtcacgcc
      301 caggtctacg acctcccagc tagcgccccg gtcgtcggaa ctcagaaaga cgccgttctc
      361 accgaccgcc cagagagaat tgccgccggc ataggcgacc ccgagaaggt tgtcgtcgac
      421 ctccgccgtg accgtctcga atccggacca gatcgtgtcg accgtaaagt aggtggtgtc
      481 cacgagcgtg tccggccacc agtaagtcgt gtcaacaatc gtatctcccg gcacgaacgt
      541 ggtgtcgatc acggtgtcgg cgggaaagtc atcaggcaga gtgtccggat ccaccgggag
      601 cgtgtcgacc gtcgtgtcgg ccgtgaggcg ttcgatttcc caatcgtagt tgaagaaccg
      661 ggcgatgaat ccgtcgtcgc ccaccgctac cgcgctcgta tcgaagacca cgatgtcgcg
      721 aaggggcccg gcgtcttcca gcgggaccgc cgaaaagcgc cgcccggcgt ccagcgagta
      781 gtagagaatg ccttcctcgc cgaccgcgaa aacccgccgc gcggtgacgc cctcgaccgc
      841 ggacagagag cgatcggtta ccgccagcag cggcaggctc caggtggtcc cgccgtcttc
      901 ggtacggcgc acgcggccgt catcaccgac cacccagccg tcctggcgac tgacgaagtc
      961 cacagcgcga agcgtccggt tggtctgact ggcctgctca atccaggtcg tgccgccgtc
     1021 ggtcgtcttc acaatgcggc cgtccacccc gaccagccag ccggtggtgg catcgacgaa
     1081 gcaggcgccc cgatacagat ttccttcggt ggtcggcgac acgagcgtcc aggcgccgcc
     1141 gccgccgccg cccggtccag tggtctcgtc gtcgctgcaa ccggcaatca ccagagccaa
     1201 gaacgcaaga gtaatcgccg ccgcagaggc gcaatgcagg tctggcaaac agcggcactt
     1261 catgtcgtta gtcctccctt acgaggttat cacacgttcc ttcagattgc ggaacttacg
     1321 ccggacctgc ttggctgtca agcgatttcg ccgtttccgc cgctccccgc agcggccgga
     1381 tccgcgcgct acaaaaaaac gcagacgaga ccgtctgcgt ttgtcggatc ggtggtcagg
     1441 agagtgaatc acgacgggga aaactcgtcg gactcgtcct gccggagcat ttcctccgct
     1501 tcggtttccg ccagttgttc gagttggcgg cgccggtcag ggcgccggcc ggcttcatcg
     1561 gatccgccgc ggaaatgccg tgcggggcgc tgccccggcc gacctttacg tttcatgcgg
     1621 gaagatgtgt gttttccgcg ctgcgcctcg tcgcgcatgc tgcctccaga gaactaagag
     1681 aatcgcagat gtgcaccttc agcctagata tcggcgaggg gggaccggac ttttatcccc
     1741 gcccgcgtcc gtgaccagga tccgggtggg gatgactatg cagcgatttt catacgaatg
     1801 accgtaatcg tcatgggtgc aagcagatgt aagcaactgc acaaccaccc cggtgacacc
     1861 gccaccgcgc caattgccga ctaatctgct gattgacaac gaaataacat gccgagacag
     1921 gggcctgagg gttcgccgcg gggacggcac aaacttcgct ctacccactc ccagacgagg
     1981 ttaccgaagg agacagcatg ttggacaacc gatatcgccg aggattctcg caggagcgcc
     2041 tggccgccat tcaggaaccc cgggtccgca aagacagccg gggatatttc ctgatgtcgc
     2101 tgtcggagaa caccaaggtg tatttcgagg atttctatcg gttcctcgaa cggacatacg
     2161 ccaaggccag ggcggagcgg tcgcggctgg atgagcgact gttcacgacg gcgccggagc
     2221 agagcgagct gatcgcctac taccgggcgc gcggcgtgat tgtcgacctc ctgatgcaga
     2281 caatcatccg gttctatacg gacggcggca acttcggcgt cgtcatgagc ccctggtgct
     2341 tcgggaccgt ggtgctggag aagctcgagg tctaccggga gcggctcagc cggggcgagg
     2401 tggaggatcc gaatatcccg gactatccct actacacgat ccgctatctc gacgagattc
     2461 acaaggcggc tctgctcgag ctgttcgatt tccccgagaa ggccttccag atgcggtggc
     2521 agtattcgga gctgctcaag cggtacagcc agatcctgag cgacatcacg acctcgctga
     2581 attcggtgct cgggacaatc aagaattatg gttcgtgacc cgcgaaccat gttttctcat
     2641 gcatccctcc tacggtgccc tccggccgcc cggccggagg gtttttttaa atcctctccg
     2701 gccgggtgcc gataaactaa ccgaacgcgc ggccgcccga aatcgggcag ccgcgcccgg
     2761 gcgacaagga gaactctccg attatggctt ccggcaccgc caaaattacc gtcacgccgc
     2821 agtacctgcc gatcctcctg gactcgctgc gggtcgactc cgtcctcgac tttgacctgt
     2881 acctgacagt caacggcaac atggtgctgt accggtcggc cgacctcccc ttcaccgacc
     2941 gcacccgccg caccctcctg gacaaccggg tcgaccggct cttcatcacc gacgacaacc
     3001 ggtcccgcta ccagcgctac atcgagcgca acctccccaa gatcatgacc gatcccggga
     3061 tccgggagga gaagaaagcg aacatcctct acgacacctc gaccaacctc gtcaaagaag
     3121 tgctggcgaa ccccacctac ggcgagaaca tccagcggac aaaggacctg gtgacaaaca
     3181 ccgtcgagtt catcctgcag gggaaagagg cctttcacac cctgctcaag atcacctcct
     3241 tcgactacta cacctacacc cattcggtca atgtctgcac gttctcgctg gccctggcgc
     3301 agcagctggg gtacggagat gagacgtttc tcaacgagct cggcgtcggg gcgctgctgc
     3361 acgacgtggg gaaatcgcgg gtgtcggagc ggattctcaa caagcgggcc cccctcaacc
     3421 cgaccgagtt cgagatcatg aagcgccatc cgcagtgggg ggcggagatt ctcgaagaga
     3481 ccgaccagat c
//
LOCUS       DAOOKS010000126         3487 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_32679_length_3487_cov_3.014569, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000126 DAOOKS010000000
VERSION     DAOOKS010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3487
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32679_length_3487_cov_3.014569"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..860)
                     /locus_tag="PK112_02910"
     CDS             complement(96..860)
                     /locus_tag="PK112_02910"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HPC11031.1"
                     /translation="MIEINLLPKGYRKKQASFSFGKTGLYAVVAAAGVVLMLVGVTVY
                     QMNQLSSLEDGIVKARQRAAMLEKDIKLVDALTDVKAKISARMAAVERLDSHRSSWVR
                     ILEDISANVPEFVWLARFTEKAPLADTAKMAAAEAGAPAGQPAPAGQAGQPNAAPATT
                     TPSVPKVMPVEVEGYAFTLNALASFMINTMRSDYFEEVELVSTKETQLGEYRAYNFVL
                     SAQLHYLSEEELQGLVAQAEAEQTEQAAPARHKSLN"
     gene            complement(868..1920)
                     /gene="pilM"
                     /locus_tag="PK112_02915"
     CDS             complement(868..1920)
                     /gene="pilM"
                     /locus_tag="PK112_02915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HPC11032.1"
                     /translation="MPFARASKSTVGLDIGASSIKLVKLDHTKSGYAVSAIGIRELPP
                     EAIVADEIRDREAVIFNIQSLIDQTDPKIKNVVISLSGHGVITDRFTIDKKTGAEAEQ
                     AILFETEQRAPFDVDDVSLDYHVVKVDDAANKMDVLLVAARKEFLQMQMEMIEDCGLR
                     PVVVDDDALAIYNAYQYNYEVDPSKVTALVNIGADVTNLTYLVDGLYHSTRDVSAGTR
                     EIFNAVQKEFRLNPELTSKAIKGEMTDSIDQDMFKATIVSASDELLAGIELAFSYFKS
                     QSRVDTIDWIVLSGGGALTPYLPELLQSKLSIPLEIANPLRKIDYDPELFQYIQPEKI
                     APLLAVSVGLAMRKVR"
     gene            complement(2182..2688)
                     /locus_tag="PK112_02920"
     CDS             complement(2182..2688)
                     /locus_tag="PK112_02920"
                     /inference="COORDINATES: protein motif:HMM:NF023444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HPC11033.1"
                     /translation="MKNLRTSSRGITLLEMMVTVVIIGIVASLAVPRFDGAMDRIRFR
                     SVNRDLTSTLRLARSSAITDKDQYGVYFDGTHRVVYLFKDLVSPTLNQFDTGDSVLRA
                     DTLPPEFNFLGTDVVGDVICFAPNGSARFTGGGNVVCLASTEAMVGIASHNILASTGR
                     VQSSTSYY"
     gene            3058..>3487
                     /locus_tag="PK112_02925"
     CDS             3058..>3487
                     /locus_tag="PK112_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11034.1"
                     /translation="MSRSRLRDLLTCAVTTAALVAAVAFSASAADRPAMLLTDQPVVQ
                     AKAAPVLSDRARAYLAGQAADTVGVWVFFTDKGIRTKDEFSARAASVAMTDKVLQRRA
                     KVDRDRVLFADLPVAAGYVDAVVAAGGHHRRTSRWLNAASF"
BASE COUNT          594 a         1154 c         1030 g          709 t
ORIGIN      
        1 cgcgagtacc agaagtaggc cacgatgaag aaggccagca cggccagcgc gatcttctgc
       61 gtctttgagt ctttgaaatc catagcctat cttcctcagt ttaagctctt gtggcgcgcc
      121 ggcgcggcct gctcggtctg ctccgcttcc gcctgcgcca ccagcccctg gagttcttct
      181 tccgaaagat agtgcagctg ggccgagagg acgaagttgt aggcccggta ctcgccgagc
      241 tgcgtttcct tcgtcgacac cagctccacc tcttcaaagt aatccgaccg catggtgttg
      301 atcataaacg aggcgagggc gttcagggta aaggcgtacc cctcgacctc gaccggcatc
      361 actttcggga cgctcggggt ggtggttgcc ggggccgcat tcggctgacc ggcctggccc
      421 gccggtgcgg gctgcccggc cggggcgccc gcttcggcgg ccgccatctt ggccgtgtcc
      481 gccaacggcg ccttctcggt aaagcgcgcc agccagacga actccggcac gttggcgctg
      541 atatcctcga ggatccggac ccaagacgac cggtggctgt cgaggcgctc cacggccgcc
      601 atccgggccg aaatcttcgc cttcacatcc gtcagagcgt cgaccagctt gatgtccttt
      661 tccagcatgg cggcccgctg gcgcgctttg acgatcccgt cctccagcga ggagagctgg
      721 ttcatctggt acacggtcac gcccaccagc atcagcacca ccccggccgc cgcgacgacc
      781 gcgtagaggc cggtcttgcc gaaggagaag ctggcctgct tcttcctgta tcccttgggc
      841 agcaggttga tttctatcat ggtcgaccta cctcaccttc cgcatggcca gtcctaccga
      901 taccgccagc agcggcgcaa ttttctctgg ctggatgtat tggaaaagct ccggatcgta
      961 gtcgatcttc cgaagcggat tcgcgatttc cagggggatg ctgagtttgg actgcagcag
     1021 ttccggcagg tagggcgtca gcgcaccgcc gcccgagagc acgatccagt cgatcgtgtc
     1081 cacccgcgac tgcgacttga agtaggagaa ggccagctcg atcccggcca gaagctcatc
     1141 ggaggccgag acgatcgtcg ccttaaacat gtcctggtcg atcgaatcgg tcatctctcc
     1201 cttgatcgcc ttgctcgtca gctccggatt cagccggaac tccttctgca cggcgttgaa
     1261 tatctcccgc gtcccggccg acacgtcgcg cgtcgagtgg tagaggccgt ccacgaggta
     1321 cgtgaggttc gtcacgtcgg cgccgatatt caccagcgcc gtcaccttgg acgggtccac
     1381 ctcgtagttg tactggtagg cgttgtagat cgccagcgcg tcgtcgtcga ccacgaccgg
     1441 ccgcagcccg cagtcctcga tcatctccat ctgcatctgc aggaattcct tgcgggcggc
     1501 caccagcagc acgtccatct tgttcgccgc gtcgtcgact ttcaccacgt ggtagtcgag
     1561 cgacacgtcg tcgacgtcga acggcgcccg ctgttcggtt tcgaacagga tggcctgctc
     1621 ggcctcggcc cccgtcttct tgtcgatcgt gaaccggtcg gtgatcacgc cgtggccgga
     1681 caacgagatg accacgttct tgatcttcgg gtccgtctgg tcgatcagcg actggatgtt
     1741 gaagatgact gcttcccggt cccggatttc gtcggcgacg atcgcctcgg ggggcagttc
     1801 gcggatcccg atcgcgctca ccgcataccc cgacttggtg tgatccagct tcaccagctt
     1861 gatgctgctg gcgccgatgt ccagaccgac cgtgcttttg cttgctcgag cgaatggcat
     1921 atgcaactct tcggttaggg gagtgattct tttctttctc tttgtatcgg cacgatccgc
     1981 cgccgattta tccctcgtct cgcaaggttt gatcgccggc cgcttcttct catatggcaa
     2041 cctgttcaga gtctgatact ttatcggcgg cttcccccaa ccctttacca ttatttccgg
     2101 cccgttctgc cgcccgtgcc aatgaaaaag gcccccctcc gagagcggaa ggaggcctcg
     2161 cggcccgacc gggccatgtc ttcagtagta gctggtggaa ctctggaccc gccccgtcga
     2221 ggccagaata ttgtgggagg cgatcccgac catagcctcc gtggaggcca ggcagacgac
     2281 gtttccgccc cccgtgaacc gcgccgaacc gtttggcgca aagcagatta cgtcgcccac
     2341 aacatcggtg ccgagaaagt tgaactcagg cggcaaggtg tcggcgcgaa ggaccgaatc
     2401 accggtgtcg aactgattca aagtcgggct gaccagatcc ttgaagaggt agacaacccg
     2461 atgcgtgcca tcaaaataca cgccgtactg atccttgtcc gtgatcgccg agctgcgggc
     2521 gagccgaagg gtggaggtca ggtcgcgatt gacgctccga aaccggattc ggtccattgc
     2581 cccgtcaaag cgcggcacgg ccagggaggc gacgatgccg atgataacaa ccgtcaccat
     2641 catctccagc aacgtgatgc cccggcttga tgtgcgcagg tttttcataa gtaacctctg
     2701 gcttatctgc ggcgccggac cgaccggtcg ccggtgcttg ctttcaactt cacgcgcgct
     2761 gtgtgcagca tgagccgtgc cccgccgccg ggatttctct ccggccgcct catcctacgc
     2821 tctctcctgt ccgataacgc cttagaggcc atcctcggac ccggcggcgg cccggcggaa
     2881 catttcgcgc gccgccgccc ttaagaactg tgggaccgct cccatactgg tgggagcggg
     2941 acaatttccc gttgacgaat tacgagaacg ggctatcttg aactcgtacc gacgtgttca
     3001 gaccgagaca aatccgccgt ggttcatggc ataaactgcg ctgaaaggaa gtactgcatg
     3061 tcacgctctc ggctccgcga tctcctcacc tgtgccgtta caaccgccgc cctggtcgcg
     3121 gctgtcgcgt tttccgcctc ggctgccgac cgcccggcca tgctgctgac cgaccagccg
     3181 gtcgttcagg cgaaagcggc gccggtcctg tccgaccgcg cccgtgccta tctggccggg
     3241 caggccgccg acacggtcgg cgtgtgggtg tttttcaccg acaagggtat ccgcacgaaa
     3301 gacgaattca gcgcccgcgc cgcctcggtg gcgatgaccg acaaggtgct gcagcgccgg
     3361 gccaaagttg accgcgaccg cgtcctcttc gccgacctgc cggtagcggc cggctacgtg
     3421 gacgccgtgg tggcggccgg cggccaccac cgccgcacct cccggtggct caatgccgcc
     3481 agcttcg
//
LOCUS       DAOOKS010000127         3475 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_32824_length_3475_cov_3.458187, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000127 DAOOKS010000000
VERSION     DAOOKS010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3475
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32824_length_3475_cov_3.458187"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(347..871)
                     /locus_tag="PK112_02930"
     CDS             complement(347..871)
                     /locus_tag="PK112_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPC11035.1"
                     /translation="MRVFLSACVLAGALALVIGCSSPQTAKIGDRVKIEYVGKTDDGV
                     VFDSTTAGQGIDFVIGQRQVIPGFENAVVGMAVGETKSVSIPPEEAYGPHRPELTHTY
                     PADSFPDNIQPELGMQLQMQTPDGQPVTLVITAIDRDSNVTVDANHHLAGKTLLFDLK
                     LVEIMAVAGDGAEE"
     gene            complement(1089..1556)
                     /gene="mscL"
                     /locus_tag="PK112_02935"
     CDS             complement(1089..1556)
                     /gene="mscL"
                     /locus_tag="PK112_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HPC11036.1"
                     /translation="MIKEFKEFAMRGNVVDMAVGIIIGAAFGSIVKSLVDDVIMPPIG
                     LLLGNVDFSSLFVTLKEGATAGPYATPALAKAAGAVTLNYGLFINSIISFLIVAFAVF
                     LLVKNVNRLKSKMETPAPAAVPTTRECPFCLSTIPKAATRCPHCTSQVQADKV"
     gene            complement(1715..3352)
                     /gene="recN"
                     /locus_tag="PK112_02940"
     CDS             complement(1715..3352)
                     /gene="recN"
                     /locus_tag="PK112_02940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HPC11037.1"
                     /translation="MMLRSLTIRNVALIDDAELEFGPGLIALTGETGAGKSVIVTALA
                     LALGGRADREFIRHGSPTAAIRAVFEDPATRTRCEVFREIAETAGTSKLTIDGAPAAV
                     AQLRERAAPMAEILGQHAGQMLMNEDNHLLFLDRFAGLEPGRGEAGRLFREWRETAEE
                     LRRVRSRRDQLAAERELLMFQQAEIEKAQVRAGEEEGLERERRILDAGRSLMESATRI
                     TDAIGGDDESILDRLQLARRELDKMAAVDPALEKQAETLAEIDFQLEDLRRHLEQYGA
                     SIEDNPARLEEINFRLDELYGLKRKYGGSEEAVLAALERIREQLKERPDTDSLIAALE
                     RQAEQCAATYADHALRLTAARRQAAADLRTLVIEELAELAVDHASFLCDFAYEEDEGG
                     VILGDRAVRPHETGLELVRFLFSANPGEPLRSLVKTASGGEISRVLLALKSAELRHGR
                     LGPGLLVFDEVDAGIGGRTANEVARKLKKLSVSCQVLVITHLHQIARLADQHFVAEKT
                     VARGKGKRTVITVRRLDGAAIRQEIDRMVALPEPPGR"
BASE COUNT          574 a         1113 c         1182 g          606 t
ORIGIN      
        1 tgttgccgac caaccccagc gcccagaggt aggcctgctt tttgtgccgc tccacgagct
       61 gcccgaaagc tttcttatct ccggcgacac atgccgaaat cagggtagtg tccgtacgca
      121 tggcgaccat gtctcgtttc aaccgtgcga ttgttccttc cagatcatta cgaactgggg
      181 ggcgggaata ttcgaaaatc gcgccgcaaa ggagcgaccg gcgcgcgggg gggccgatag
      241 gccccgggga agctcgcggt aacgtgcggt aacgcaatgg gttggccggc cgaacaagaa
      301 aacggggcga aaccgccccg tgagacaacg gattggctga cgcgccctat tcctcggccc
      361 cgtcacccgc gaccgccatg atctccacca gcttcagatc aaacagaagg gtcttgccgg
      421 cgaggtggtg attggcatcg accgtaacat tgctgtcccg atcgatggcg gtgatgacga
      481 gagtcaccgg ctggccgtcg ggcgtctgca tctggagctg catcccgagc tccggctgga
      541 tgttgtcggg gaagctgtcg gccgggtagg tgtgggtgag ttcgggccgg tggggtccgt
      601 aagcctcttc cggcgggatg gagacggact tggtctcgcc gacagccatt ccgacgacgg
      661 cgttttcaaa gccggggatc acctgccgtt ggccgatgac gaaatcgatg ccctggccgg
      721 ccgtcgtgct gtcaaacacc acgccgtcat cggtctttcc cacatattcg attttcacgc
      781 gatcgccgat cttggcggtc tgcgggctgc tgcagccgat gacgagcgcg agcgcgcctg
      841 cgagaacgca tgccgacagg aaaactctca caagaacctc ctcaggagtg tgaccggttc
      901 gaggcccgcc gactgcggga atgccgccgg cgccgatttc tccaaaacta cggtacggac
      961 cgccatcggg caagaagatt tccggcgccg gcacacggcg gagcgccggc cgattccgcg
     1021 gccgcgaaaa agctgcggcg ccgccccggc cggagcggcg ccgtgcggta tcgagccgag
     1081 gtgcgcaatc agaccttgtc ggcctgcacc tgcgacgtgc agtgcgggca gcgcgtcgcg
     1141 gccttaggaa tggtcgacag acagaacgga cactcccggg tggtcgggac cgccgccgga
     1201 gccggcgtct ccatcttgct cttgagacgg ttgacattct tcacgagcag gaagacggcg
     1261 aaagcgacga tgaggaagct gatgatgctg ttgatgaaga ggccgtagtt gagggtgacc
     1321 gccccggccg ccttggcgag ggcgggagtg gcgtagggtc cggctgtcgc accctccttg
     1381 agcgtgacaa agaggctgga gaaatcgaca ttgccgagca gcaagccgat cgggggcatg
     1441 atgacgtcat cgacaagcga cttgactatc gagccgaacg cggcgccgat aatgataccg
     1501 accgccatgt cgacgacatt gccgcgcatg gcgaattctt tgaattcttt gatcatggcc
     1561 atgaactgtt ctctccttca cataaagggc caaagaacac aggcaacggt gagccaagag
     1621 aaatatccgg agccgctcct cggcgatgca actgaaaact tgtctccggc cgtccataaa
     1681 gctctccggc cggcgggcgc cgggacggga gcgcttagcg gcccggcggt tcggggaggg
     1741 cgaccatgcg gtcgatttcc tgccggatgg cggcgccgtc gagccgccgc accgtgatca
     1801 cggtccgctt tcccttgccc cgggcgacgg tcttctccgc gacgaagtgc tggtcggcca
     1861 ggcgggcgat ctggtgcagg tgcgtgatca ccaggacctg gcacgagacc gacagtttct
     1921 tcaatttgcg ggccacctcg ttggccgtcc ggccgccgat gccggcgtcc acctcgtcga
     1981 acacgagcag accgggtccc agccggccat ggcgaagttc ggcggacttg agggccaaaa
     2041 ggacccgcga gatctcgccg ccggaagcgg tcttgacgag cgaccggagg ggttcgccgg
     2101 gattggccga aaacaggaag cggaccagtt ccaggccggt ttcgtgcggc cgcacggcgc
     2161 gatcgccgag gatcacgccc ccctcgtcct cttcgtatgc gaagtcgcac aggaaactgg
     2221 cgtggtcgac ggcgagctcc gcgagttcct caatgacgag ggtgcgcaga tcggccgccg
     2281 cttggcggcg ggcggccgtg agccggaggg cgtggtcggc gtatgttgcg gcgcactgtt
     2341 cagcctgccg ttccagcgcc gcgatgagcg aatcggtgtc gggacgttcc ttgagttgtt
     2401 cacgaatgcg ctcgagcgcg gccagcaccg cctcctccga cccgccgtat ttcctcttaa
     2461 ggccgtagag ctcatcgagg cggaagttga tttcctcgag ccgcgccggg ttgtcctcga
     2521 tcgaagcgcc gtactgctcg aggtggcggc gaaggtcctc gagctggaag tcgatctcgg
     2581 cgagcgtctc ggcctgcttt tccagggcgg ggtcgaccgc ggccatctta tccagctccc
     2641 ggcgcgccag ttggaggcgg tcgaggatgc tttcgtcgtc gcccccgatc gcgtcggtga
     2701 tcctggttgc cgattccatg aggctgcggc cggcatcaag gatgcgacgt tcgcgctcaa
     2761 gtccttcttc ctcgccggcc cgcacctgcg ccttctcgat ctccgcctgc tggaacatca
     2821 ggagctcgcg ctcggcggcc agttggtcgc ggcggctgcg cacgcggcgc agttcctcgg
     2881 ccgtctcgcg ccactcgcgg aacagccgcc ccgcctcgcc gcgcccgggc tcgaggccgg
     2941 cgaagcgatc gaggaacaac aggtggttgt cctcgttcat gagcatctgc ccggcgtgct
     3001 ggccgaggat ttccgccatc ggcgcggccc gctcgcggag ctgggccaca gcggccgggg
     3061 cgccgtcgat cgtcagcttc gaggtccccg ccgtctccgc gatctcgcgg aagacctcac
     3121 accgggtgcg ggtcgcggga tcttcgaaca ccgcccggat ggcggcggtg ggggagccgt
     3181 ggcggatgaa ttcgcggtcg gcccggccgc ccagggcgag ggcgagggcg gtgacgatga
     3241 ccgatttgcc ggcccccgtt tcgccggtca gggcgatcag accggggccg aactctagct
     3301 cggcgtcgtc gatcagcgcg acattgcgga tggtcagcga gcgcagcatc attgcacccg
     3361 gcggtgaatc tccgccatca gccgcgcgat cgcctcccgg tccccggccg cttccgcctc
     3421 ggcgcgcggg tcgggctggc cctgctgcgc gcgcattgcc tcggcgatct cgtgc
//
LOCUS       DAOOKS010000128         3474 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_32835_length_3474_cov_2.882422, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000128 DAOOKS010000000
VERSION     DAOOKS010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3474
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32835_length_3474_cov_2.882422"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            565..>3474
                     /locus_tag="PK112_02945"
     CDS             565..>3474
                     /locus_tag="PK112_02945"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HPC11038.1"
                     /translation="MRFLRFMLVVTATALSGAVTVAAQVSEGGTPPSFGKAVGAAIDR
                     YVTAPIDVAALLEEDARTPKDVPFRFGYPFDVRLGLDNAGTWEVLADGSRLWRLQIEC
                     PGAASINLIFDRFWLPDGARLFIYNADRSHVIGAFTSRNNKDYGSFATQPVRGDVSVL
                     EYWEPAGLNAQPELRVSRIVHAYRNLFARDFLKDFGESGACNNNVRCPEWAAFDPLIR
                     SVALITTGGGFRLCSGAMINNVRQDLTPYFLTANHCLGGSDTWVFIFNYESPACSNVD
                     GPTYMTVSGSTLRANLAYSDFALLELSEQPPDSYNVYYAGWNAVNAAADSVIAIHHPS
                     GDIKKITFDYNAVTSTSYLGTAVPGDDSHWRIGQWEDGTTEPGSSGSPIFNRQGQIIG
                     QLHGGYASCASITSDWYGKFAKSWSYGSTAATRLRDWLDPDNTGALSLGGRESAGISI
                     THDPLPDTRDTVNDYAVLAAITSDVPLDPDSLLLYYEIGAANYEDTLTPTGNPDEFVG
                     YIPAQAPGTVVSYRLLAVDSDGKADTTDSFSFRVIDYGLTLAPAADTLSGPVADSVWY
                     ALQIVNTGVYTDSYALAVSGNAWTTEIFDAAGTTPLLSTGPLAADSVFAFTVRVIVPP
                     SVYGAADTALVTATSVGDPVYTAASRLRTVSDGEPLAIPFEDEFPAAALSAAHWVQTT
                     GAEANAVALAEPSEPYSLNLDGAPAGADTVMSQVINLAGQGNLIVRYFYERTGGGDSP
                     EANDDLFVEYRDSAGVWQLLQQHLGSGPDMTTFSEVELPLPPAAYHSAFRLRLRSIGT
                     AGAYDDWFVDDLYVGPPQPFKLSLDPAFASQYGPAGDTMQYTLQIANRGSQADQYSFA
                     DSGGAWPVSFWDDAGVAPVGGSGMIAAGDSARFVVKVEVPADAPMNAVDSCRVRAVSA
                     GDPLMFDDSRLVSFSAGPPGGFPWYEPFPVDTVIVSRWTSIVGAAVSAAALFPPSA"
BASE COUNT          532 a         1293 c         1072 g          577 t
ORIGIN      
        1 gcagttcctc cagtggtacg tggccgagca ggtcgaggaa gtcgcttcga ccagcgagct
       61 cgcgggcatg gtgaagcttg ccggcacggc cggccagctc ctccagctgg agagccggat
      121 ttaccggatg gtcgaagcgc gcagctgagt tcggcgcgga tggcgtcgga tgaataagcg
      181 gccgcccctg cgggcggccg ttttctttgc ccgcccggcg gtctatcgta gcgaaattct
      241 acccacatta tcatgaattc ccttgaccgc ccgcgggtcc gccgcttaat taactatatg
      301 gatttctgaa ttcgccatcc ggaccgcccg cggccggcgc cgacagtccc ggttccgccc
      361 cgtccgcgcg ggcccgcctc cggcgacaca gcccatgggg aatcgccgat agtgccaaac
      421 cgtcagccac catccgccgc accgccgccc gccgcggccg ccatcgtttc tcgggcggga
      481 ccgatcccgg cgcggccgac ggtcgacggc ttctccccac gactcaccaa ccgttccacc
      541 aaccatcagc ctgcggggtg cgcaatgagg ttcctgaggt tcatgctggt cgtcaccgcg
      601 accgcgctgt cgggcgcggt aaccgtggcc gcgcaggtca gcgaaggggg cacgccgccg
      661 agtttcggca aggcggtggg cgccgcaatc gaccgctacg tcaccgcgcc gatcgacgtg
      721 gccgcgcttc tcgaggagga cgcgcggacg cccaaagatg tgcctttccg attcggctac
      781 ccgttcgatg tccggctcgg tctggacaac gcgggcacgt gggaggtgct ggccgacggc
      841 agccggctgt ggcggctgca gatcgaatgc cccggcgccg cgtcgatcaa cctgatcttc
      901 gaccggttct ggctgcccga cggcgcccgg ctgttcatct acaacgccga ccgctcccac
      961 gtcatcggcg ccttcacgag ccgtaacaac aaggactacg gctccttcgc cactcagccg
     1021 gtgcgcggcg acgtgagcgt gctggagtac tgggagccgg ccgggctgaa cgcgcagccg
     1081 gaactgcggg tctcgcgcat cgtccacgcc taccgcaatc tgttcgcgcg ggattttctc
     1141 aaggatttcg gcgaatcggg cgcctgcaac aacaacgtcc gctgccccga gtgggcggcc
     1201 tttgatcccc tcatccgctc ggtggccctg atcacgaccg gcggcggctt ccgcctctgt
     1261 tcgggcgcga tgatcaacaa cgtgcggcag gacctcaccc cctatttcct caccgccaac
     1321 cactgcctcg gcggctccga cacctgggtg ttcattttca actacgagag cccggcctgc
     1381 agcaacgtcg acggccccac ctacatgacg gtgtcgggtt ccaccctccg ggcgaatctc
     1441 gcctactcag acttcgcgct cctggagctg tccgagcagc cgccggattc ctacaacgtc
     1501 tactacgccg gctggaacgc cgtcaacgcc gccgccgaca gcgttatcgc catccaccac
     1561 ccctcgggcg acatcaagaa gatcactttc gactacaacg cggtgacctc cacgagctac
     1621 ctggggaccg cggtgcccgg cgacgattcg cactggcgga tcggccagtg ggaggacggc
     1681 acgaccgagc ccgggtcctc cgggtcgccg atcttcaacc gccagggaca gatcatcggc
     1741 cagctccacg gcggctacgc ttcctgcgcg agcatcacct ccgactggta cgggaagttc
     1801 gccaagtcgt ggagctacgg gtcgaccgcc gccacccgcc tgcgcgactg gctggacccc
     1861 gacaacacgg gtgcgctcag tctgggcggc cgggagagcg ccggcatctc catcacccac
     1921 gatccgctcc ccgacacccg cgacacggtc aacgactacg ccgtcctcgc cgccatcacc
     1981 agcgacgttc ctctggaccc cgactcgctc ctgctctact atgaaatcgg ggcggccaac
     2041 tacgaggata cgctcacccc gaccggcaac cccgatgagt ttgtcggcta cattcccgcg
     2101 caggctcccg gcacagtcgt cagctaccgc ctgctggcgg tcgacagcga cggcaaggcg
     2161 gacaccaccg acagtttcag cttccgcgtc atcgactacg gcctgaccct ggcgcccgcc
     2221 gccgatacac tctccggccc ggttgccgat tcagtctggt acgccctgca gattgtgaac
     2281 accggcgtgt acacggactc ctacgccctc gcggtgtcgg gcaacgcctg gactaccgaa
     2341 atcttcgacg ccgccgggac gacgccgctt ttgtccaccg gcccgctggc ggccgactcc
     2401 gttttcgcgt tcacggtccg cgtcatcgtg ccgccctcgg tgtacggggc cgccgacacg
     2461 gcccttgtca cggccacgtc cgtcggcgat ccggtctaca ccgcggccag ccggctccgc
     2521 accgtctccg acggcgagcc gctggcgatt ccgttcgagg acgagttccc ggcggccgcg
     2581 ctgagcgccg cgcactgggt ccagaccacc ggggccgagg ccaacgccgt cgcgctcgcc
     2641 gaaccctccg aaccgtacag cctcaacctc gacggcgccc cggcgggagc cgacacggtc
     2701 atgtcgcagg tgatcaatct cgcgggccag gggaatctga tcgtgcgcta tttctacgag
     2761 cgcaccggcg gcggcgactc ccccgaggcc aacgacgacc tcttcgtcga gtaccgcgac
     2821 agcgccggcg tgtggcagct cctgcagcaa cacctcggtt ctggccccga catgacgact
     2881 ttctccgagg tggaactccc gcttccgccg gccgcttatc acagcgcgtt ccgcctgcgc
     2941 ctgaggagta tcgggacggc gggcgcctac gacgactggt tcgtcgacga cctctacgtc
     3001 ggcccccccc agcccttcaa gctctctctg gacccggcct tcgccagcca gtacggcccg
     3061 gccggcgaca ccatgcagta cacgctgcag atcgccaacc gggggtcgca ggccgaccag
     3121 tacagcttcg ccgactcggg cggcgcgtgg cccgtgtcgt tctgggatga cgccggggtg
     3181 gccccggtcg gcggcagcgg catgatcgcc gccggcgatt ccgcccggtt tgtggtcaag
     3241 gtcgaggtgc cggccgacgc cccgatgaac gccgtcgact cctgccgtgt gcgcgccgtc
     3301 tccgccgggg acccgctcat gttcgacgac agtcggctgg tttcgttctc ggccggcccg
     3361 cccggcggat ttccgtggta cgaacccttc ccggtcgata cggtcatcgt ctcccggtgg
     3421 acaagcattg tcggcgcggc ggttagcgcg gcggcgcttt tcccgccctc ggcc
//
LOCUS       DAOOKS010000129         3473 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_32843_length_3473_cov_3.878291, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000129 DAOOKS010000000
VERSION     DAOOKS010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3473
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32843_length_3473_cov_3.878291"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1384
                     /locus_tag="PK112_02950"
     CDS             <1..1384
                     /locus_tag="PK112_02950"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HPC11039.1"
                     /translation="PYAAVTPEVDTGGPDGATAITYAITADSLVRFGGVSVRGVKRYP
                     EYTAVRELKFKPGDVYRRQDLIDSERRLFESGYFSTLQLQQEAQQSDRYRPDFVLQVR
                     ERKPYYLSFTTGAGQSEAADLTWSVTSGAGKRNLFGSRRLEFYNRVEFSLGADARLTD
                     HLYRLRYTEPWLLGFRMPLAVTLEYQPRIRHEAQDFDVEEWNASLSTTRRVGRLTDVT
                     AGIEYQSVDISGVPAELEEAVRAEVEGLSIRRRLYLNLRRDSRLDVFVPRQGSVRDLR
                     TEYYGGFLGGDEHFYRLEGSWSFYRPVWPGWISATRFRSGYAHAFGDSELVPSKDRFF
                     LGGANTIRGFAENTLAPTLADSLPGANFTVVCNQEFRWKTLQVFAVVPLLNKLLENMP
                     LWQTVFIDIGNGFTRVSEFSFSRLAYSYGTGVQIISPAGPIRLDYARRIETDDIPFDD
                     RWHFTILFAF"
     gene            1505..2680
                     /locus_tag="PK112_02955"
     CDS             1505..2680
                     /locus_tag="PK112_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006716007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HPC11040.1"
                     /translation="MDQIYLDHNATTPVDPEVVDAMLPYLSHTFGNASSLHYFGREAK
                     AALERAREEVAAFISCDPGELYFTSGGTESDNMAVLGAAFQRRDRRRHAVYGATEHHA
                     VMEACEYLVHRHGFTADAAPVDSEGFVSPRRLARLVTDQTAVVSIMHANNETGTIQDL
                     KPLVQAAHEKGAWFHTDAVQSCGKIEINVRDLGLELLSLTGHKIYGPKGTGALFIRDG
                     IKLTPLMYGGSHEKKRRPGTENVAGAVGLAKALEIARRRMEEDWIRLSDLADFFIDRV
                     LTTIPHTYLNGTRMGRIPQVCNISFTGVEGESILLALDLEGVACSSGSACTSGATEPS
                     HVLVAMGKDRVVAQGAIRFSLGRSTNREQLDYVLAKLPPIIERLRSLSPLYQERMKS"
     gene            2723..>3473
                     /gene="mnmA"
                     /locus_tag="PK112_02960"
     CDS             2723..>3473
                     /gene="mnmA"
                     /locus_tag="PK112_02960"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF001138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HPC11041.1"
                     /translation="MPPRVLVAMSGGVDSSVAALLLKEEGYEVVGAHMKLWDYVDVGG
                     DRHRDGRCCSLDAVTDCRLVCDRIGAPFYVLNMSAPFRAAVIENFVSEYRAGRTPNPC
                     VLCNTDIKWSGFLHKAREIGCDYIATGHYAVIEQGENGRWRIRKGVDATRDQSYVLWG
                     VSQDALAMTLMPLGRRYKKDVRAFAARVGLRTAAKPESREICFVADNDYRRFLREWEQ
                     KHGLKRRPGEIVHEDGRVLGRHAGTVDFTVGQ"
BASE COUNT          585 a         1254 c         1052 g          582 t
ORIGIN      
        1 tccctacgcc gccgtcaccc cggaggtcga taccggcgga ccggatggcg cgacggcgat
       61 cacttatgcg atcaccgccg actccctcgt gcgattcggc ggcgtctccg tgcgcggcgt
      121 caaacggtac ccggagtata cagccgtccg cgaactcaag ttcaagccgg gcgacgtcta
      181 ccggcgccag gacctcatcg attccgagcg gcggctgttc gaatccggct acttctcgac
      241 cctgcaactg cagcaggagg cgcagcagtc cgaccgctac cgccccgatt ttgtccttca
      301 ggtgcgcgag cgcaaaccgt actacctgtc gttcacgacc ggggccgggc agtccgaggc
      361 cgccgacctg acctggagcg tgacctccgg cgccggcaag cgcaacctct tcggctcgcg
      421 ccgcctcgaa ttctacaacc gcgtcgagtt ctcgctcggg gccgacgccc gcctcaccga
      481 ccacctctac cgcctccgct acaccgaacc ctggctgctg ggattccgca tgccgctggc
      541 ggtgaccctc gaataccagc cgcgcatccg ccacgaggcg caggatttcg acgtcgagga
      601 gtggaacgca tcgctctcga ccacccgccg ggtcggccgc ctcaccgatg tcaccgccgg
      661 catcgaatac cagtcggtcg acatcagcgg cgtcccggct gaactcgaag aggccgtgcg
      721 cgccgaggtc gaggggctgt ccatccgtcg ccgcctctat ctgaatctgc gccgggacag
      781 ccgcctcgat gtcttcgtgc cccgccaggg ctcggtgcgc gacctccgca ccgaatacta
      841 cggcggcttt ctgggcggcg acgagcactt ctaccgcctc gagggctcgt ggtcgttcta
      901 ccgcccggtc tggccgggct ggatctcggc gacccggttc cgctccggct acgcgcacgc
      961 cttcggcgat tccgaactgg tccccagcaa agaccgcttc ttcctggggg gcgccaacac
     1021 gatccgcgga ttcgccgaaa acacgctcgc cccgactctg gccgacagcc tgccgggcgc
     1081 caattttacc gtcgtgtgca accaggaatt ccgctggaaa accctccagg tcttcgccgt
     1141 cgtgcccctg ctgaacaagt tgctggagaa catgcccctc tggcagacgg tcttcatcga
     1201 catcggcaac gggttcaccc gggtcagcga attcagcttc agccggctgg cctattccta
     1261 cggcaccggc gtccagatca tctcgcccgc cgggccgatc cggttggact acgcccgtcg
     1321 gatcgagacc gatgatatcc cctttgatga tcgctggcat ttcactatat tgtttgcgtt
     1381 ctgaccgccc tcccggttgc ggcgggaacc ggtcacggcg tgagccggtt tttccctagc
     1441 aaccggcgcg gtccgtcccg gccgtcggtg gttcgctgaa gagaaagggt gcacaataga
     1501 gactatggat caaatttacc tcgatcacaa cgcgaccacg ccggtggacc cggaagtggt
     1561 tgacgccatg ctgccgtacc tgtcgcacac gttcggcaac gcgagcagcc tgcactactt
     1621 cggccgcgaa gccaaagcgg cgctcgagcg ggcccgtgag gaggtcgccg ccttcatcag
     1681 ttgcgacccc ggcgagttgt actttacctc gggcggcact gagtccgaca acatggccgt
     1741 actcggcgca gccttccagc ggcgcgaccg ccgccggcac gcggtgtacg gcgcgaccga
     1801 gcaccacgcc gtcatggagg cctgcgagta tctggtccac cgtcacgggt tcacggccga
     1861 cgccgccccg gtcgacagtg aggggttcgt ctcgccccgg cggctggccc gcctcgtgac
     1921 cgatcagacg gcggttgtgt cgatcatgca cgccaacaac gagaccggga ccatccagga
     1981 cctcaaaccg ctcgtgcagg ccgcccacga aaagggggcg tggtttcaca ccgacgccgt
     2041 gcagtcgtgc gggaagattg aaatcaacgt gcgcgatctc gggctcgaac tcctgtcgct
     2101 gacgggtcac aagatatacg gccccaaggg gacgggcgcg ctgttcatcc gcgacggcat
     2161 caaactcacg ccgctcatgt acggcggctc gcacgagaag aagcgccgcc ccgggaccga
     2221 aaatgtcgcc ggcgccgttg ggctggccaa agcgctcgag atcgcccgcc gccgcatgga
     2281 agaggactgg atccgcctga gcgacctggc cgacttcttc atcgaccggg tgcttaccac
     2341 gatcccgcac acctacctca atggcacccg catgggacgc attccccagg tctgcaacat
     2401 ctctttcacc ggcgtcgaag gggagtcgat cctgctggcc ctcgacctgg agggcgtcgc
     2461 ctgctcctcc ggttcggcct gcacctccgg cgccaccgaa ccgtcccacg tgctggtcgc
     2521 catgggaaag gaccgcgtgg tcgcgcaggg agcgattcgc ttctccctcg gccgctccac
     2581 caaccgggag cagctcgact acgtgctggc caagctgccg ccgatcatcg agcgcctgcg
     2641 gtcgctttcg ccgctgtacc aggaacggat gaaatcgtag gcccggaccg ccgggtcccc
     2701 ggcccgcagg aagacgattg ccatgccccc ccgcgttctg gtcgccatgt ccggcggcgt
     2761 cgactcctcc gtcgccgccc ttctcctcaa agaagagggg tacgaagttg tcggcgccca
     2821 catgaaactg tgggactacg tcgacgtggg cggcgaccgg caccgcgacg gccgctgctg
     2881 ctcgctcgac gccgtcaccg attgccgcct cgtgtgcgac cgcatcggcg ccccgtttta
     2941 cgtcctcaac atgtccgcgc cgttccgcgc cgccgtcatc gaaaacttcg tctccgagta
     3001 ccgcgccggc cgaaccccga atccgtgcgt cctctgcaac accgacatca agtggagcgg
     3061 tttcctccac aaagcccgcg agatcggctg cgattacatc gccaccggcc actacgccgt
     3121 catcgaacag ggcgagaacg gccgctggcg catccgcaag ggggtcgacg ccacccgtga
     3181 ccagtcctac gtgctctggg gcgtgtccca ggatgcgctc gccatgaccc tcatgccgct
     3241 cggccgccgg tacaagaagg acgtacgcgc attcgccgca cgcgtcggtc tccgcaccgc
     3301 tgcgaaaccc gaatcgcgcg aaatctgctt cgtcgccgac aatgactacc gccgcttcct
     3361 ccgcgagtgg gaacagaaac atgggctcaa gcgccggccg ggggagatcg tccacgagga
     3421 cggccgggtg ctgggccgcc acgccggtac ggtcgatttc acggtcggcc agc
//
LOCUS       DAOOKS010000130         3466 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_32926_length_3466_cov_3.044562, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000130 DAOOKS010000000
VERSION     DAOOKS010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32926_length_3466_cov_3.044562"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            85..1260
                     /locus_tag="PK112_02965"
     CDS             85..1260
                     /locus_tag="PK112_02965"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HPC11042.1"
                     /translation="MRRAAWAAALLLVLGAADVQADKGKDKDRIRSQQEELERIRKDV
                     ERSEKKLDSLRSAELQTTEQISRYDQKITANKKVIGRLTRQLADVHEQMQATEDRLGQ
                     SRDRLERARRKYLGDIRQFYAKATGRTARPLWSGPAKGLEENRQIVYLAALAQFESDN
                     VADAGAYLSETLAMLDTLASQRTKVQSLKKDKETATALDLSQREKREKALEKIRRTKL
                     AEGDKMLTLQQAADEIERVIALLEEQRERRESRPSEPAPPSAFATLKGQLPPPAPGKV
                     TVPFGPAQDPVTKLKSYSPGITIAAALGAAVRAVAAGSVAYVGTLRGYGRFVIISHDS
                     DYYTTYAGLGEPQVRTGDAVTGTTVLGRVGPEGQVKFELRQGREPLDPVLWIRLDAF"
     gene            1250..2368
                     /locus_tag="PK112_02970"
     CDS             1250..2368
                     /locus_tag="PK112_02970"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11043.1"
                     /translation="MPFDISQFQPRAWEVMARSVESDRVAGTYLLCGPDGCGRWGLAV
                     SFAALLNCEARTRTAGGALRPCGGCRNCRLIFALNFEGLAVAAPLPPHKGEDEAVELT
                     NEVIDLKRREPFAVLSATANLTIPIDTARRIKRTMATKPPPGVYRTIIFYQMERMRPE
                     AADALLKMIEEPPSQAVILLIAEKAERLLPTIQSRSRKVTLRSSPPEVVAEYLRAQYG
                     LAEARAELAARTAEGIAGRAVQAAESGDDEASGRVTAFQLFRSLFEDSGPETVARLTA
                     ALGERDRGEAEYLLHLWQSLARDCMQLAVAGAGGAVVNTDFRAELERLAEHFADPATA
                     VRLAEEIKIALAHFRLNVHIHGALAALALRTKAAASRH"
     gene            2410..3309
                     /gene="ricT"
                     /locus_tag="PK112_02975"
     CDS             2410..3309
                     /gene="ricT"
                     /locus_tag="PK112_02975"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HPC11044.1"
                     /translation="MAELYLVEFKGSRKEFFFNRYYHALTLNSYVIVEVERGEELGYL
                     KKKIETEVSFGEGNHPRSILRPAGSEDAARWEYLRDQEEGYKYEVIDIISRHGLPMKV
                     VDVECQFDGNKMTVFFTADHRVDFRELVKDLAARYRMRIELRQIGVRDEARRLGGYGI
                     CGREQCCNSFIRNFEPISTQHAREQDLPLNPSKISGNCGRLLCCLRYEADMYRKVKSR
                     FPEPGTMVRTRLGEGVIERIDVFNEEAIIRDAERVTFRVGLKDILERERKVEEAAAGP
                     ARDLEAGETEAEALKKLDEGEGR"
     gene            3331..>3466
                     /locus_tag="PK112_02980"
     CDS             3331..>3466
                     /locus_tag="PK112_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003623515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HPC11045.1"
                     /translation="MAKPIYLTTPIYYVNDRPHIGHAYTTIAADLLARFHRLAGREAF
                     F"
BASE COUNT          625 a         1093 c         1251 g          497 t
ORIGIN      
        1 gcttgctgaa atcgccggct tctgcctcgg ggccgcgctc ctcggagtgg tcagcgggta
       61 cctcggggtg cggaggatgc tcgcgtgaga cgtgcggcgt gggcggcggc cctcctgctg
      121 gtcctggggg cggcggacgt gcaggcggac aagggcaagg acaaggaccg catccgcagc
      181 cagcaggaag agctggagcg catccgcaag gacgtcgagc ggagcgagaa gaagctcgac
      241 tcgctgcgca gcgccgagct gcagacgacg gaacagatct cgcggtacga ccagaagatc
      301 accgcgaaca agaaagtgat cgggcggctg acgcgccaac tggccgacgt gcacgagcag
      361 atgcaggcga ccgaggaccg tctcgggcag tcgcgcgacc ggctcgaacg ggcgcgccgc
      421 aaatatctcg gcgatatccg ccagttctac gcgaaggcca ccgggcggac ggcccggccg
      481 ctctggtcgg ggcctgcgaa ggggctggag gaaaaccgcc agatcgttta cctcgccgcc
      541 ctcgcccagt tcgaatcgga caacgtcgcc gacgccggcg cctacctgag cgagacgctc
      601 gccatgctcg acacgctcgc tagccagcgg accaaggtcc agtcgctcaa aaaggacaaa
      661 gagacggcga cggcgctcga cctgagccag cgagagaagc gggagaaagc gctggagaaa
      721 atccgccgga ccaagctcgc cgagggggac aagatgctta cgctgcagca ggcggccgat
      781 gaaatcgagc gcgtgatcgc gctgctcgaa gagcagcggg agcggcggga aagccggccg
      841 agcgagcctg cgcctccctc ggcgttcgcc accctgaagg ggcagctccc accgccggcg
      901 ccggggaaag tgaccgtgcc gttcgggccg gcccaggatc cggtcaccaa gctgaagtcg
      961 tactcgccgg gaatcacgat cgcggccgcg ctcggggcgg cggtccgcgc ggtggcggcc
     1021 ggatcggtcg cctatgtcgg gacgttgcgg ggatacgggc ggtttgttat cataagccat
     1081 gacagcgact actacaccac ctacgccggg ctcggcgagc cccaggtgcg caccggggac
     1141 gcggtgacag ggaccaccgt gctcgggcgg gtggggccgg agggccaggt aaagttcgag
     1201 ttgcgccagg gtcgcgaacc cctcgatccg gtgctgtgga tacgcctcga tgccttttga
     1261 catctcgcag tttcaacccc gcgcctggga agtgatggcg cggtcggtcg agtccgaccg
     1321 ggtggccggc acctaccttc tctgcgggcc ggacggatgc ggccggtggg gactggcggt
     1381 ctcgttcgcc gcgctgctca actgcgaggc gcggacgcgg accgccggcg gcgcgctccg
     1441 gccctgcggc gggtgccgga actgccgcct gattttcgcg ctcaatttcg aaggcctcgc
     1501 ggtcgccgcg cccctgcccc cgcacaaggg cgaggacgag gcggtcgagc tgaccaacga
     1561 ggtgatcgac ctcaagcgcc gggaaccgtt cgcggtgctc tcggctacgg ccaatctgac
     1621 gatcccgatc gataccgcgc gccggatcaa gcggacgatg gcgaccaaac cgccgcccgg
     1681 cgtctaccgg accatcatct tctaccagat ggagcggatg cgcccggagg cggcggacgc
     1741 gcttttgaaa atgatcgagg aaccgccgtc gcaggcggtc atcctcctca tcgcggagaa
     1801 agccgagcgg ctgctgccga cgatccagtc gcggtcgcgg aaagtgacgc tgcgttcgtc
     1861 tccccccgag gtggtcgcgg agtacctgcg ggcgcagtac ggtctggcgg aggcgcgcgc
     1921 cgagctggcc gcgcgcacgg ccgaggggat tgccggccgg gcggtgcagg cggccgagtc
     1981 gggcgacgac gaggcctcgg gacgggtgac cgcattccag ttgttccggt cgctgtttga
     2041 ggacagcggg ccggagaccg tggcgcggct gacggccgcg ctgggagagc gcgaccgcgg
     2101 cgaggcggag tacctgctcc acctgtggca gtcgctggcc cgggactgca tgcagctggc
     2161 ggtggccgga gcgggcggcg ccgtggtgaa caccgatttc cgggccgaac tggagcggct
     2221 ggccgaacac ttcgccgatc cggcgaccgc cgttcgcctc gccgaggaga tcaagatagc
     2281 gcttgcccat tttcggctga acgtacatat tcatggggcg ctggcggcgc tggccctcag
     2341 gaccaaggcg gcggcgagtc ggcattgacc ggaaagcggg caccccccgg gcaagcgggg
     2401 attttttgta tggcggaact gtacctcgtg gaattcaagg gctcccgcaa ggagttcttc
     2461 ttcaatcgct actaccacgc gctcaccctc aacagctacg tgatcgtcga ggtcgagcgc
     2521 ggagaggagc tcggctacct caagaagaaa atcgagaccg aggtctcgtt cggggagggg
     2581 aaccaccccc gctcgatcct ccggccggcc ggcagtgaag acgcggcccg gtgggaatat
     2641 ctccgcgacc aggaagaggg gtacaagtac gaggttatcg acatcatctc ccggcatgga
     2701 ctgccgatga aggtggtgga cgtagagtgc cagttcgacg ggaacaagat gacggtgttt
     2761 ttcacggccg accaccgggt ggatttccgc gagctggtca aggacctggc ggcccgctac
     2821 cggatgcgga tcgagctgcg ccagatcggc gtgcgcgacg aggcgcggcg gctgggcggc
     2881 tacggcatct gcggccggga gcagtgctgc aattccttca tccgcaattt cgaaccgatt
     2941 tcgacccagc acgcgcggga acaggatctc cccctgaacc cctcgaaaat ctccggcaac
     3001 tgcgggcggc tcctgtgctg cctgcgctac gaggccgaca tgtaccgcaa agtaaagagc
     3061 cggttccccg agccgggaac gatggtgcgg acgcggctgg gggaaggcgt gatcgagcgg
     3121 atcgacgtgt tcaacgagga ggcaatcata cgcgatgcgg agcgggtgac gttccgggtc
     3181 gggttgaagg atattctgga gcgggagcgc aaagtggagg aggcggcagc cggcccggcg
     3241 cgcgacctcg aggccgggga gacggaagcc gaggcgctca agaagctcga cgaaggcgag
     3301 gggcggtaaa cccgaacgcg gagaccagct gtggccaagc cgatttacct cacgacgccc
     3361 atttactacg tcaacgaccg cccgcacatc gggcacgcct acaccacgat cgcggccgac
     3421 ctgctcgcgc gctttcaccg cctggccggg cgcgaggcgt ttttcc
//
LOCUS       DAOOKS010000131         3461 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_32992_length_3461_cov_3.284204, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000131 DAOOKS010000000
VERSION     DAOOKS010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3461
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32992_length_3461_cov_3.284204"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /gene="ybeY"
                     /locus_tag="PK112_02985"
     CDS             <1..375
                     /gene="ybeY"
                     /locus_tag="PK112_02985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HPC11046.1"
                     /translation="RLFRAVARGERRKAGEVNLVFTGGRRMRRLNRTYRGRDKATDVL
                     AFPLHEGAGEQADEIYGEVYIAVPVARAQAAAYGVGLGEEILRLACHGWLHLFGYDHH
                     TPAEAAAMRTREERYLAAVRGA"
     gene            380..1639
                     /locus_tag="PK112_02990"
     CDS             380..1639
                     /locus_tag="PK112_02990"
                     /inference="COORDINATES: protein motif:HMM:NF015436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HPC11047.1"
                     /translation="MLDLLYVFAIIAGFATAYVISLFSLAVYVDPEEVEKLSPNQSAR
                     RRGFVKKLANDPLALVQSATVYKSFALIIVTVAMYQLLPRLGAISGWPTTVVRLFGML
                     LTWLLYIFFVEYMPRRSAAAADRARLLRHLWLVGIIYFLFFPLVGRYRRALQRIRPPK
                     DVTGEDKEDLVERALETLAEQAGIGERILEEEKKEMIGQIFQLDQTVAREIMIPRIDI
                     VGIEKSMTFPEIRRLVMRDGHSRYPVYEGSIDRILGLAYVKDMFNHLPEPGEEFRVTD
                     HMRKAYFIPGTKVIGELLREFKHKRMHIAVVVDEYGGVSGLVTLEDIIEEIFGEIRDE
                     HDWEQEQIVKLTDGAFLVNAGLLVEDLQDFLDTDFEQSDYDTVGGLIYDLVGSVPREG
                     QVIRWHDLEFEVERVQGQRIVAVKVRR"
     gene            complement(1706..2125)
                     /locus_tag="PK112_02995"
     CDS             complement(1706..2125)
                     /locus_tag="PK112_02995"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPC11048.1"
                     /translation="MNTAKTTILVVDDEPAIRQILLRSLSSAGFDTTTATDGADALLK
                     MAQQPFDIVITDITMPHMDGVALLGEIRSRFPDTKVIFITGQRLALMDKAVREADVDG
                     LISKPFRNHEIVGQLRQLCLCRTHHRAPSDPVPPAPA"
     gene            complement(2228..2446)
                     /locus_tag="PK112_03000"
     CDS             complement(2228..2446)
                     /locus_tag="PK112_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11049.1"
                     /translation="MALATKPVTRCPFCKETILEGAVKCKHCHSELSAPPAARPKRLA
                     RLNTFRIGFLVGVVFTLILVILVYRHFA"
     gene            complement(2509..2844)
                     /locus_tag="PK112_03005"
     CDS             complement(2509..2844)
                     /locus_tag="PK112_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012928936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HPC11050.1"
                     /translation="MQQQPPQQQIAVEFDEKAAEGIYANLALIMHSPTEIIIDFARVM
                     PRLPKAKVLSRIIMTPMHAKLLHQALTENLKKFEAQFGEIKTFGFPGGGKGPIGFESG
                     VRFVDPEKE"
     gene            2980..>3461
                     /locus_tag="PK112_03010"
     CDS             2980..>3461
                     /locus_tag="PK112_03010"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HPC11051.1"
                     /translation="MSLSAERIAEVIARLGKARVLILGDIMLDEYLFGRVDRISPEAP
                     VPVVEISHDKLLLGGAANVAANIRALGDEPLLVGVVGEDEAAVKLSQLLKQHGIGADY
                     CVCDRSRQTTIKTRIMAHSQQVVRADRENRHELHPDVERMVLAKFTAAAESVNAVIIS
                     "
BASE COUNT          611 a         1043 c         1223 g          584 t
ORIGIN      
        1 cggctcttcc gggcggtggc ccggggcgag cggcggaaag cgggggaggt caacctcgtg
       61 ttcaccgggg ggcgccggat gcgccgcctg aaccgcacgt accgcgggcg ggacaaggcc
      121 accgacgtgc tcgctttccc gctccacgag ggggccgggg agcaggcgga cgagatctac
      181 ggcgaggtct atatcgccgt gcccgtggcc cgggcccagg ccgccgccta cggggtcggg
      241 ctgggcgaag agatcctgcg gctggcctgc cacgggtggc tgcacctttt cgggtacgat
      301 caccacaccc cggccgaggc cgccgccatg cgcacgcggg aggagcgcta cctcgccgcg
      361 gtgaggggag cctagcccta tgctggacct gctctacgtt ttcgccatca tcgccgggtt
      421 tgccaccgcc tacgtgatct cgctgttctc gctggccgtc tacgtggacc cggaggaggt
      481 cgagaagctt tcccccaacc agtcggcccg gcggcggggg tttgtgaaga agctcgcgaa
      541 cgatccgctc gcgctggtgc agagcgccac ggtctacaag tcgttcgcgc tgatcatcgt
      601 caccgtggcc atgtaccagc tccttccccg cctgggtgcg atttcggggt ggccgacaac
      661 tgtcgtgcgc ctcttcggca tgctcctgac ctggctcttg tacatcttct tcgtcgagta
      721 catgccgagg cgatcggcgg ccgcggccga ccgggcgcgg cttttgcggc acctctggct
      781 cgtcggcatc atctacttcc tgttcttccc gctcgtcgga cgctaccggc gggcgctgca
      841 gcgcattcgc ccgccgaaag acgtgaccgg cgaggacaaa gaggacctcg tcgagcgggc
      901 gctggagacg ctcgccgagc aggcggggat cggcgagcgc attctcgagg aagagaagaa
      961 ggaaatgatc gggcagatct tccagctcga ccagacggtg gcccgggaga tcatgatccc
     1021 ccgcatcgac atcgtgggga tcgaaaaatc gatgacgttt ccggagatcc gccggctggt
     1081 catgcgcgac ggccactcgc gctacccggt ctacgagggg agcatcgacc gcatcctcgg
     1141 cctcgcctac gtgaaggaca tgttcaacca cctgcccgag ccgggggagg agttccgggt
     1201 gaccgaccac atgcgcaagg cgtatttcat accggggacc aaggtcatcg gcgaactgct
     1261 ccgcgagttc aagcataagc gcatgcacat cgcggtggtc gtggacgagt acggcggcgt
     1321 gtcggggctg gtgacgctcg aggatatcat cgaggaaatc ttcggcgaaa tccgggacga
     1381 gcacgactgg gagcaggagc agattgtcaa gctgaccgac ggggcctttc tcgtcaacgc
     1441 cggacttctg gtcgaggacc tgcaggattt cctggacacc gacttcgagc agagcgacta
     1501 cgacacggtg ggcgggctga tctacgacct ggtcgggtcg gtgccccggg aggggcaggt
     1561 gatccggtgg cacgatctcg aattcgaggt cgagcgcgtc caggggcagc ggatcgtcgc
     1621 cgtcaaggtg cggcggtagg ggacgatcgg ttcacggaac gggttatctt gaacagcgga
     1681 agccggggcc ggcgccgcgg ggcgcctagg ccggcgcggg cgggaccggg tcggacgggg
     1741 cgcggtggtg ggtgcggcac aggcagagct ggcgcagctg gcccacgatc tcatggttgc
     1801 gaaagggctt gctgatcagc ccgtcgacgt ccgcctcgcg caccgccttg tccatgagag
     1861 cgagccgctg gccggtgatg aagatcactt tggtgtcggg gaaacgggac cggatctcgc
     1921 cgagcagcgc gactccgtcc atgtggggca tggtgatgtc ggtgatgacg atgtcgaagg
     1981 gctgctgggc cattttgagg agggcgtccg cgccgtcggt ggccgtcgtt gtgtcgaagc
     2041 cggccgacga aaggctgcgc aggagtattt gccggatcgc cggctcatcg tcgacgacca
     2101 ggatggtggt tttcgctgtg ttcacgaaaa ggccctcagg acgcagccgc gcctcaaccg
     2161 gcgccgctat cctgttgtag tatcggcagg tggcgcagga tccttgagtg gacagccagg
     2221 gacatgttca ggcgaaatgc cggtagacga gaatcacgag gatgagggtg aagaccacgc
     2281 cgacaaggaa gccgatgcgg aaagtgttga gccgggccag gcgcttggga cgggccgccg
     2341 ggggggcgct caactccgag tggcagtgct tgcacttgac cgccccctcc aggatggtct
     2401 ccttgcagaa ggggcagcgg gtgaccggtt tggttgccag cgccatgccc catgggtagc
     2461 ggagcgcgcg ccgcgaggca acaaaaaaca gccgggcccg ggcgcccctt actccttctc
     2521 cggatcgaca aaacgcacgc ccgactcaaa accgatcgga cctttgcccc cgccggggaa
     2581 accgaaagtc ttgatctcgc cgaactgcgc ctcaaacttc ttgaggttct cagtcagggc
     2641 ctggtggagg agtttggcgt gcatgggggt catgatgatg cgcgagagga ccttggcctt
     2701 cggcaggcgg ggcatcacgc gggcgaagtc gatgatgatt tcggtggggg agtgcatgat
     2761 cagggcgagg ttggcataaa tgccctcggc ggccttctca tcgaactcca cggcgatttg
     2821 ctgctgcggc ggctgctgct gcatcgggag gctcctttcc gcggacttca aagtgcttgt
     2881 gcatgatcaa gcatggtaat atattccgcc ggcaagtcaa cagagaagcg gcggaaaggg
     2941 ccgccgcgga ccgatcgcgg ccaagaggag gcattgcccg tgtcgttgag tgcggaacgc
     3001 atagcggaag tgatcgcgag gctggggaag gcacgggtgc tcatcctggg ggatatcatg
     3061 ctcgacgagt acctcttcgg acgcgtcgac cgcatctcgc ccgaggcgcc cgtgccggtc
     3121 gtggaaatct cgcacgacaa gctcctgctg gggggcgccg ccaacgtggc cgcaaacatc
     3181 cgggcgttgg gcgacgaacc gctcctcgtc ggggtggtgg gtgaagacga ggcggcggtc
     3241 aaactcagcc aactgctcaa acagcacggc atcggggccg attactgcgt gtgcgaccgg
     3301 tcccgccaga ccaccatcaa gacccgcatc atggcccaca gccagcaggt ggtgcgggcc
     3361 gaccgggaaa accgccacga actccacccc gacgtggaac ggatggtgct ggccaagttc
     3421 acggccgccg cggagtcggt caacgccgtg attatctccg a
//
LOCUS       DAOOKS010000132         3453 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_33077_length_3453_cov_4.197763, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000132 DAOOKS010000000
VERSION     DAOOKS010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3453
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33077_length_3453_cov_4.197763"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1565)
                     /locus_tag="PK112_03015"
     CDS             complement(<1..1565)
                     /locus_tag="PK112_03015"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11052.1"
                     /translation="MPVRRFQRRGAYLPTRKRDLVSVSNLIVETSLVSAASAEEAHRR
                     QNLLGGTFEGHLLRLKALTEEELVGAIRSGAGCDAIALSGLPIPESVIGYLPRRFAEQ
                     RCLLPVAFDPAANRLTVACLDPRDEGLAAEIRAVTGVQEIHLAAALEPVLRCRIIEAY
                     RNRPEVDDTRGAAGPDAPPAPRRPILLVGAIDPAVETLARALALEGQSVILVETVDEA
                     LAEIAAAAPRAVVVRSRPNETHAVLEDHLRATAPQCPVCFAEGVADLITAAPPAELTG
                     SLFTAALAMWPAGAAPADELKVRRVAERVDLTAQLCDRLKLSPRDRLLVVHAAIAAGA
                     PERPPSDRRPPEVAAILAHRESEIADTYADRLPLAVLGGSILRLVDTLADRWGQLKDP
                     TPELYRALTAELRPLRGQAFLPQVVDAFIAMVSERIPTPRPFDRASTQALVLGDADGY
                     VGALAGALAGAGFEVLRAGTAARFIDLYGSRWPDFLVIVSSGSARDPRRMVDDLADRG
                     IAFDTVPALLARAR"
     gene            1847..>3453
                     /gene="pruA"
                     /locus_tag="PK112_03020"
     CDS             1847..>3453
                     /gene="pruA"
                     /locus_tag="PK112_03020"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011189732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HPC11053.1"
                     /translation="MYRIPQPKNEPILNYAPGSPERAALKEAIKFLKANPIDVPLVIG
                     GQEIKTDHKIKITAPHDHNLLLGHYYQGGEKEVKMAVEAAVEAQKSWQHMPWEQRSAI
                     FLRAADLISTKYRYIMNAAAMLAHSKTAHQAEIDAVAELADFYRFNCWCAQQIYDTFQ
                     PESSPGVWNRIDHRPLEGFVFAVTPFNFISINGNLPVAPAIVGGVSVWKPASTATYTS
                     HWIMKILREAGLPDGVVNLLFAKGRDIGELVLKSRHLAGIHFTGSTPVFQMMWKTVGD
                     NLPNYNTYPRLVGETGGKDFIIAHPSADVDAVVANTIRGAFEFQGQKCSAASRCYFPE
                     SLWPTIKQKMIAELGTIKMGDVENFGNFMGAVIDGAAFKSITEYIDYAKQHKDKCEIV
                     FGGRYDDSKGWFIEPTVVATTDPHFRLMVEEIFGPVLTIYVYKDAEYANTLHLCDQTS
                     PYSLTGAVFARDRMAVALAERTLRHAAGNFYINDKPTGAVVGQQPFGGGRASGTNDKA
                     GAIQNMMRWISPRTVKENFIPPTDYRYP"
BASE COUNT          650 a         1099 c         1179 g          525 t
ORIGIN      
        1 cgggcgcggg cgagcagggc ggggacggtg tcgaaagcga ttccccggtc cgcgagatcg
       61 tccaccatcc ggcgggggtc gcgggccgac ccggagctga cgatgacgag aaaatccggc
      121 cagcggctgc cgtacaggtc gatgaaccgg gcggcggtgc cggcgcggag gacctcaaac
      181 ccggcgccgg cgagtgcgcc cgccaaagcg ccgacatagc cgtcggcgtc gcccagcacc
      241 agggcctggg tggaggcgcg gtcgaagggc cgcggggtcg ggatccgctc gctcaccatg
      301 gcgatgaaag cgtcgaccac ctgcgggagg aaagcctgtc cgcgcagggg tcggagctcg
      361 gccgtgagcg cgcgatagag ctcgggggtg ggatctttca gttgccccca ccgatccgcg
      421 agagtatcga ccagtcgaag gatactgccg ccgaggacgg ccagcgggag gcggtcggca
      481 tacgtgtcgg cgatctcgct ctcccggtgc gcaaggatcg ccgcgacctc gggcggccgg
      541 cggtcgcttg ggggtcgctc gggcgcaccg gcggcgatcg ccgcgtgaac cacgaggagc
      601 cggtcgcgcg gcgaaagctt gaggcggtcg caaagctgtg cggtcaggtc caccctctcg
      661 gccactcggc gtaccttcag ttcgtcggcc ggggcggcgc ccgccggcca catggccagc
      721 gcggcggtga aaaggctgcc ggtcaattcc gcaggcggag ccgcggtgat caggtcggcg
      781 accccctcgg caaagcaaac cggacactga ggagccgtgg cacggaggtg gtcctcgagg
      841 acggcgtgag tttcgttcgg ccggctccgg acgacgacag cccgcggcgc cgcagcggca
      901 atttccgcca gcgcttcatc gaccgtctcg acaaggatga cgctctggcc ctcgagggca
      961 agcgcccggg cgagggtctc gacagccggg tcgatcgcgc cgacaaggag gatcggccgc
     1021 ctcggcgccg ggggagcatc ggggcccgcc gcgccccgtg tatcgtccac ctccggccgg
     1081 ttgcgataag cctcaatgat gcggcagcgg aggaccggct ccagcgcggc tgcgaggtga
     1141 atctcctgca cgccggtgac cgcccgaatc tcggccgcca gaccttcatc gcggggatca
     1201 agacaagcga ccgtcaaccg gttcgccgcc ggatcgaacg ccaccggcag caggcaacgc
     1261 tgctcggcga accggcgggg aagatagccg atcacggatt cgggtatggg gaggccggaa
     1321 agcgcgatcg cgtcgcatcc ggccccgctg cggatggcgc cgaccaactc ctcctccgtc
     1381 aacgccttga ggcgcaacag gtggccctcg aaagtgccgc cgaggaggtt ttggcgacgg
     1441 tgcgcctcct cggccgaggc ggccgagacc aggcttgtct ccacgatgag attgcttacc
     1501 gacacgagat ccctctttcg agtcgggagg tatgcgccgc gccgctggaa gcggcgcacc
     1561 ggcatagtcc tgcctctata cccataatgg tcggacaatc ccagtggaag ttgagcgggg
     1621 gaataacagg gggcaaaaga ggacatgggt agtcctgttg ccgcgcaccg gtggacggga
     1681 ggttgccgcg agtcggggcg cgctccgctt gcgaacgagg caacaggtgc ttgccccgcc
     1741 ccggctttct gcttatcatg ttgtccccgt cggcgggccg gtcgaggccg gagggcagcc
     1801 ggggcgacaa aggataatat tactcgagac ataaggagac gctgtcatgt accgcattcc
     1861 ccagccgaaa aatgaaccga ttctgaacta tgctcccgga tcgccggagc gggcggcact
     1921 gaaggaggcg atcaagttct tgaaagccaa cccgatcgac gtgccgctcg tgatcggcgg
     1981 acaggagatc aagaccgatc ataagatcaa gatcacggcc ccccacgacc acaatctcct
     2041 cttgggacat tactaccagg gcggcgagaa agaggtcaag atggcggtgg aggcggcggt
     2101 cgaggcgcag aagagctggc agcacatgcc ctgggagcag cgctcggcga ttttcctcag
     2161 agctgccgac ctcatctcca ccaagtaccg gtacatcatg aatgcggcgg cgatgctggc
     2221 gcattccaaa accgcgcacc aggccgaaat cgacgcggtg gcggaactgg ccgatttcta
     2281 ccgcttcaac tgctggtgcg cccagcagat ctacgacaca ttccagccgg agagttcgcc
     2341 gggcgtctgg aaccggatcg atcaccggcc gctcgagggt ttcgtgttcg cggtgacgcc
     2401 tttcaatttc atctcgatca acggtaacct cccggtcgcc ccggcgatcg tggggggcgt
     2461 ctcggtgtgg aagccggcct cgacggcgac ttacacctcg cactggatta tgaaaatcct
     2521 ccgcgaggcg gggttgcccg acggcgtggt gaacctgctg tttgcgaagg ggcgggacat
     2581 cggcgaactc gtgctcaaga gccgccacct cgccgggatc catttcaccg gctcgacgcc
     2641 ggtgttccag atgatgtgga agacggtcgg cgacaacctt ccgaactaca acacctaccc
     2701 gcgcctggtc ggcgaaacgg gcggcaagga tttcatcatc gcgcatccct cggccgacgt
     2761 cgacgcggtg gtggccaaca cgattcgcgg cgccttcgag ttccaggggc agaagtgctc
     2821 ggccgccagc cgctgctatt tccccgaatc gctctggccg acgatcaagc agaagatgat
     2881 cgccgaactc gggacgatca agatggggga tgtcgagaac ttcggcaact tcatgggggc
     2941 cgtgatcgac ggcgcggcgt tcaagtcgat caccgagtac atcgactacg cgaagcagca
     3001 taaggacaag tgtgagatcg tcttcggcgg ccgctacgac gattccaagg ggtggtttat
     3061 cgagcccact gtggtggcca caaccgaccc gcacttccgc ctcatggtcg aagaaatctt
     3121 cgggccggtg ctcacgatct acgtatacaa ggacgccgag tacgccaaca cgctgcacct
     3181 gtgcgaccag acctcgccct acagcctcac gggcgcggtg ttcgcgcgcg accggatggc
     3241 ggtggcgctg gccgaacgga cactgcgcca cgccgcgggc aatttctaca tcaacgacaa
     3301 accgaccggc gcggtggtgg ggcagcaacc gttcggcggc gggcgcgcca gcgggaccaa
     3361 cgacaaagcc ggggcgatcc agaatatgat gcggtggatc tcgccgcgca cggtgaagga
     3421 gaatttcatt ccgccgaccg actaccgcta ccc
//
LOCUS       DAOOKS010000133         3437 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_33280_length_3437_cov_3.973093, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000133 DAOOKS010000000
VERSION     DAOOKS010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3437
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33280_length_3437_cov_3.973093"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="PK112_03025"
     CDS             complement(<1..546)
                     /locus_tag="PK112_03025"
                     /inference="COORDINATES: protein motif:HMM:NF040495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic aminobenezylarsenical efflux permease
                     ArsG family transporter"
                     /protein_id="HPC11054.1"
                     /translation="MDSFAVAAATAFWLGLLTSVSPCPLATNIAAMSYVGRRIERPRL
                     VLLSGVLYTVGRLAAYAALAALIVSSLTAVPELARWLQRWMNVVLGPILILAGLFLID
                     LIPLRLPGIGVGRRLEQRVDRLGIWGGGLLGVMFALSFCPVSAALFFGSLIPLALEQR
                     SGFLMPVLYGLGTALPVVVFAA"
     gene            complement(546..1031)
                     /locus_tag="PK112_03030"
     CDS             complement(546..1031)
                     /locus_tag="PK112_03030"
                     /inference="COORDINATES: protein motif:HMM:NF040494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrophenyl compound nitroreductase subunit ArsF
                     family protein"
                     /protein_id="HPC11055.1"
                     /translation="MNPVAFTMLLFGCLLGAALPASAAEEPAAPAADTAAIDSVEPAP
                     AEAPVQVIATYFHGNRRCATCRKLEAYAQEAVTGGFPDEIAAGVLQWRVVNFDEDANA
                     HFLEDYELFSQAVILSRLEGGKETVWKNLDRIWTLVGDQEAYLAYVRSELRTFLAAGA
                     D"
     gene            complement(1062..1295)
                     /locus_tag="PK112_03035"
     CDS             complement(1062..1295)
                     /locus_tag="PK112_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020885568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPC11056.1"
                     /translation="MKKIQVFGPGCAKCEELARLAAAAVAELGLACEVEKVSDIRAIM
                     EAGVMITPALAVDGRVLVAGKVPSLAKLKEILA"
     gene            complement(1314..2612)
                     /locus_tag="PK112_03040"
     CDS             complement(1314..2612)
                     /locus_tag="PK112_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015774086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HPC11057.1"
                     /translation="MDWRREWRPLAVLTAVFLGLYFLPLTGPRAASAWSEALALTQWY
                     AREHVILCLLPAFFIAGAIGVFVGKASVMKYLGAGADQRLAYGVASVSGSVLAVCSCT
                     VLPLFAGIYRMGAGLGPAAAFLYAGPAINVLAVILTARVLGLSLGVARAVGAVLFSIV
                     IGLLMHFFFRREAREREAAAAVLPADEPVRPLGQTALFFGLMIAVLVFATWGRPAQPV
                     GFWSAVYAIKWWLTGAAALALGALLWRRFGLAWWKVAAPAAVTLALAWLFPDRPQLVF
                     AVGVIGLSVVLAASPGEPGEWFAQTWGFAKQILPLLVIGVLAAGALLGRPGEEGLIPQ
                     TWVAAAVGGNSLQANLLASVIGAFMYFATLTEIPILQGLIGSGMGPGPALALLLSGPA
                     VSLPSMLVIRSVIGTKKTVVYVLLVVGMATLSGLIYGALL"
     gene            complement(2690..3010)
                     /locus_tag="PK112_03045"
     CDS             complement(2690..3010)
                     /locus_tag="PK112_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015862372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPC11058.1"
                     /translation="MDSATRQLFASRARVIKALAHPTRLFIVDELSRGERCVCALRDL
                     VGADISTVSKHLAVLKAAGIVDDDKRGLQVWYRLRVPCILNFFGCVEEVLRTHRRGVA
                     SGHE"
     gene            3257..>3437
                     /locus_tag="PK112_03050"
     CDS             3257..>3437
                     /locus_tag="PK112_03050"
                     /inference="COORDINATES: protein motif:HMM:NF017051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HPC11059.1"
                     /translation="MELGIIGTSIWQQNMRLLETLTIDREGKLDRLVQLKAALGVDEL
                     IYLSTCNRVEFIFASR"
BASE COUNT          700 a         1139 c         1122 g          476 t
ORIGIN      
        1 cgcggcaaag accaccaccg gcagggccgt ccccagcccg tagagcacgg gcatgagaaa
       61 accggaccgc tgttcgagag cgagcggaat caagctgccg aaaaagaggg ccgctgagac
      121 cgggcagaac gacagggcga acatcacccc gagcagcccg cccccccaga tccccagccg
      181 gtcaacccgc tgctcgaggc ggcggccaac gccgattccg ggaaggcgga gcgggatcag
      241 gtcgatgagg aacagcccgg cgagaatcag aatcggcccg agcacgacgt tcatccagcg
      301 ctggagccag cgggccagct cggggacggc ggtgaggctg ctgacgatga gcgcggccag
      361 cgccgcgtag gccgccagcc gccccaccgt gtacagcaca ccggacagga gcaccaggcg
      421 ggggcgttca atccgccgcc ccacatagct catggcggcg atgttggtcg ccagcgggca
      481 cggggacacg gaggtcagca aacccagcca gaaagccgtg gcggcggcaa ccgcgaacga
      541 atccatcagt cggccccggc ggcgagaaag gtccgcagct cactgcggac gtaggcaagg
      601 taggcctcct gatcgccgac cagcgtccag atccggtcga ggtttttcca gactgtctcc
      661 ttcccgccct cgaggcgcga gaggataacc gcctggctga acagctcgta gtcctcgagg
      721 aagtgcgcgt tggcatcttc atcgaagttg acgacgcgcc actggaggac gccggcggcg
      781 atttcatcgg ggaatccccc ggttaccgcc tcctgcgcat aagcctcgag cttccggcag
      841 gtggcgcacc ggcggttgcc gtggaagtac gtggcgatga cctggaccgg cgcttcggcc
      901 ggggccggtt cgacggagtc gatcgcggcc gtgtcggccg ccggggccgc gggttcctcg
      961 gcggcggagg cgggaagcgc ggcgccgagc aggcacccga acagaagcat ggtgaatgcg
     1021 accgggttca tgcggttctc ccttctggtt ccgcctgaga gtcaggcgag gatttctttc
     1081 agtttggcca gggacgggac tttgcccgcg accaacaccc ggccgtcgac cgccagcgcc
     1141 ggtgtgatca taacgccggc ctccatgatc gcccggatgt cggacacttt ttccacctca
     1201 caggcgaggc cgagttcggc caccgcggct gcggccagcc gggcgagttc ctcgcatttc
     1261 gcgcagcccg gcccaaaaac ctggatcttc ttcatgttgt atcgcccttt gccttagagt
     1321 aatgcgccat agatcagtcc gctgagggtc gccatcccga ccaccagcag gacgtacacc
     1381 acggtttttt tggtcccaat caccgagcgg atcacgagca tgctcggcag cgaaacggcc
     1441 gggccggaca gaagcagggc gagcgccggc cccggtccca tccccgagcc gatcagcccc
     1501 tgcaggatcg ggatctcggt gagcgtcgca aagtacatga aagcgccgat gaccgaggcg
     1561 aggaggttcg cctgaaggga gttgccgccc acagccgcgg ccacccaggt ttgcgggatc
     1621 agcccctcct ctcccggccg gccgagaagc gctcccgcgg cgagcacgcc gataacgagc
     1681 agcggcagaa tctgtttggc gaacccccaa gtctgggcga accactcccc cggttcgccc
     1741 ggcgacgccg cgaggacgac cgagagcccg attacgccga cggcaaacac gagttgcggc
     1801 cgatcgggga agagccaggc gagcgcgagg gtcacggccg ccggcgcggc cactttccac
     1861 caggcaagac cgaatcgtcg ccacaacagc gcccccagcg cgagcgcggc ggcgccggtc
     1921 agccaccact tgatcgcgta cacggccgac caaaacccga ccggctgggc cgggcggccc
     1981 caagtggcga acaccagcac ggcgatcatc agaccgaaaa agagcgccgt ctggcccagc
     2041 ggtcggactg gttcatcggc cggcaacacg gcggccgcgg cctcccgctc ccgcgcctcg
     2101 cggcggaaga agaagtgcat gagcaggccg atgacgatgc tgaacagaac cgccccgacc
     2161 gcccgcgcga cccccagcga caggccgagc acgcgcgcgg tcagaatgac ggcgagcacg
     2221 ttaatcgcgg ggccggcgta gaggaaggcg gcggccggac cgagcccggc gcccatgcgg
     2281 tatatcccgg cgaacagcgg cagcacggtg cacgagcaca ccgccagcac cgaacccgaa
     2341 accgacgcga cgccgtaggc gagccgctgg tcggcccctg cgccgaggta tttcatcacc
     2401 gatgctttcc cgacgaacac gccgatcgcc ccggcgataa agaacgccgg cagaaggcac
     2461 aggatcacat gctcgcgggc ataccactgg gtgagcgcaa gcgcctcaga ccaggccgag
     2521 gccgcccggg ggccggtcaa ggggaggaaa tacagaccga ggaagacggc ggtgagaacc
     2581 gccagcggcc gccattcgcg cctccagtcc atcggctcac ccacccgccc caatgatgga
     2641 tccccgcccg gcgaccgacc cgccggccga acgacccccg gagagacgct cactcatggc
     2701 cggaggccac tccccggcga tgggtgcgga gaacctcctc gacacacccg aaaaagttca
     2761 ggatgcatgg gacgcgcagg cggtaccaca cctgcagacc ccgcttgtcg tcgtcgacaa
     2821 taccggccgc tttcagcacg gcgaggtgct tggagacggt cgagatgtcg gccccgacca
     2881 ggtcgcgcag ggcgcacacg caccgttcgc cgcgcgagag ctcatcgaca atgaacagcc
     2941 gagtaggatg ggcgagcgct ttgatgaccc gggcgcgaga ggcgaaaagt tgtctggtcg
     3001 cagaatccac acagtgcctt tgacgagtat tcctttattt ggcaaaatag ccaaatagtt
     3061 gggcgaggca agggattttt gggcggcggg tgcgggaaaa tccacactct tgagtatgtt
     3121 ctcgtggaga gttaattccc acttcttgta ccttttttca caactatgac agtcggatga
     3181 caaaacaatt cggcgggatg atacctttgc ccacaatgga aatggacgcc ggcggaactc
     3241 aagcctaagg acggacatgg aactcggcat catcgggacc tcaatctggc agcagaacat
     3301 gcggctgttg gagacgttga cgatcgaccg tgagggcaaa cttgaccgtc tggtgcaact
     3361 gaaagcggcg ctcggggtcg acgaattgat ttacctgtcg acctgcaacc gggtggagtt
     3421 catttttgcc tcgcgtg
//
LOCUS       DAOOKS010000134         3434 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_33320_length_3434_cov_3.443326, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000134 DAOOKS010000000
VERSION     DAOOKS010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33320_length_3434_cov_3.443326"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1697
                     /locus_tag="PK112_03055"
     CDS             <1..1697
                     /locus_tag="PK112_03055"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HPC11060.1"
                     /translation="DTNIPGSGLCIWHYDAGTAAGTGSNRYPPYRIALEQADGQFQLE
                     YAGSSGDAGDPFPGATGNRNFHDYSTPATNLNDDLSPLYGLVSRIGVWDVSNADSVMY
                     AELDIEYSRPWIVEASSGFVFNDDAPRGDGDGVVEAGEEIHFYCYIRNFMRYSYNVRA
                     ALASGSPRIEWVTPAVSFGTNLLVDIQYNTDPIIFRVKDSLTAAIDTFTVTIVTDSLP
                     GGTFGSEFVKEFQFSKQVGATQVLVVDDDNGAEYQQEYVDALRGRGIPSRVWNVQSQG
                     SPDSLALSGYRIVFWHTGIDTSTGAFSAANVAAMKGYLNAPDHHLCLGTYALVKLAAL
                     DNAFVADYLKASHAGNLYFPQYLGVPGTAIGNGTEYRPITGAPNAAAANAYMNVLSGG
                     QSIFTGGPSGQIRGIAYAGPTYKTVLLTFAPELLNDNAGGTTMPKDTLISRIFAYFGG
                     QATSIHDGSPFPVMPSSFTLEQNYPNPFNPSTTIRYTLRSTGADFRSTPRTKLAVYNA
                     LGQRVATLVDEVQIPGSYAVDWDGSDGRGHRAASGVYFYRLERGDEAQTMKMVLLK"
     gene            1901..2353
                     /locus_tag="PK112_03060"
     CDS             1901..2353
                     /locus_tag="PK112_03060"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HPC11061.1"
                     /translation="MREFLRTKGFKMTPQRELVFRAFFELGDHVTVDELFEKVRRQDR
                     SIGYSTVWRNLKLICRVGLAEEVNVGDGVTRYDRVTHVPHGHLYCQECRRLTEFPVEE
                     IVAVLAGVAEQNDFRADSFKIEIHGYCEECRERRRRALGSGGGAAPPK"
     gene            2384..2614
                     /locus_tag="PK112_03065"
     CDS             2384..2614
                     /locus_tag="PK112_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HPC11062.1"
                     /translation="MTYLSRMSPGQRGRIVGFVDDSPLARRLLELGIIPGRLITYLRN
                     APLSDPMQVQVGSSCLTLRHQEASLVAVEPEE"
     gene            2659..>3434
                     /locus_tag="PK112_03070"
     CDS             2659..>3434
                     /locus_tag="PK112_03070"
                     /inference="COORDINATES: protein motif:HMM:NF014474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HPC11063.1"
                     /translation="MPAPEITKTREIAICGNPNCGKTTIFNAITGLAQQVGNYPGVTV
                     ERIRGQFAHGGRRYVLADIPGTYSLSAFSPDEYIACAALTGSLDASPAPDAIICVLDG
                     TQLERGLYFLLQVLEIGRPTVAAVNMVDIAAKRGLTIDYAALERLTGVPVVPVVGSRG
                     EGIARLVALAAQAADAPPLPPRRRYRAEVENLIERLQATPGNGHRSRAEYLRIVFDAD
                     GPAERRFLAAAGGAGRALLERGRSLIRERAGTLAAAETAPL"
BASE COUNT          627 a         1102 c         1127 g          578 t
ORIGIN      
        1 ttgacacgaa catcccggga tccggcctgt gcatctggca ctacgacgcc ggcacggccg
       61 ccggtacggg cagcaaccgg tacccgccct accgcatcgc gctggagcag gcggacggcc
      121 agttccagct tgagtacgcc gggagctccg gcgatgcggg ggatccgttc cccggcgcca
      181 ccggcaaccg caacttccac gactacagca cgccggccac gaatctcaac gacgacctct
      241 cgccgctcta cggcctcgtg tcgcgtatcg gcgtgtggga tgtctccaac gccgattcgg
      301 tcatgtacgc cgaactcgac atcgagtact cgcggccctg gatcgtcgaa gcctcgagcg
      361 ggttcgtctt caacgatgac gcgccgcgcg gggacggcga cggcgtcgtc gaagccggcg
      421 aagagatcca cttctactgc tacatccgca atttcatgcg gtactcctac aatgtgcggg
      481 cggcgctcgc cagcgggtcg ccccggatcg agtgggtgac cccggccgtc tccttcggca
      541 cgaacctgct ggtcgacatc cagtacaaca ccgatcccat catcttccgg gtaaaggaca
      601 gcctgacggc ggcgattgac acgttcacgg tgacgatcgt gaccgactcg ctccccggcg
      661 ggacattcgg cagcgagttt gtcaaggagt tccagttctc caaacaggtg ggcgccaccc
      721 aggtgctggt ggtcgacgac gacaacgggg ctgagtacca gcaggagtat gtggatgcgc
      781 tgcggggccg ggggattccc agccgggtgt ggaatgtcca gtcccagggc tcgcccgact
      841 cgctggctct gagcggctat cggatcgtct tctggcacac gggcatcgat acctcgaccg
      901 gcgctttcag cgcggctaat gtcgccgcga tgaaagggta cctgaacgcc cccgaccatc
      961 atctgtgcct ggggacctac gcgctggtaa aactggccgc cctcgacaac gccttcgtcg
     1021 cagactacct caaggcgagc catgccggca atctctactt ccctcagtat ctcggcgtcc
     1081 ccgggacagc catcggcaac ggcacggagt accggccgat caccggcgcg ccgaatgcgg
     1141 cggcggccaa cgcttatatg aatgtgctca gcggcggcca gagcatattc accggcgggc
     1201 cgagcggaca gatacgcggc atcgcctacg cgggcccgac ctacaaaacc gtgttgctca
     1261 ccttcgcccc cgagctgctc aacgacaacg cgggcggtac gaccatgccg aaagacacac
     1321 tcatcagccg catcttcgcc tacttcggcg gccaggccac gtccatccac gacggttccc
     1381 cgttcccggt catgccgtcc tcgtttaccc tcgagcagaa ttacccgaac ccgttcaacc
     1441 cgagcaccac gatccggtac acgctgcgga gcaccggagc ggatttccgg tccacgccgc
     1501 gcacgaaact ggcggtctac aacgccctcg gccagcgggt ggcaacgctg gtcgatgaag
     1561 tccagattcc gggatcctac gcggtcgatt gggacgggtc ggacggaaga gggcatcggg
     1621 cggcctccgg agtctatttc taccgcctcg aacggggtga cgaggcccaa acaatgaaaa
     1681 tggttctcct taagtagagc aaagttgcac taccgaaaaa gaagcagccg ccggcccacc
     1741 ccggcggcta tctttttgca gtagaatagg atatattcat gtgaaaaatg ccacttagcc
     1801 gctcgatttt ccttgacatc aggccgaaag agagtaatat atgctgatga aattgaaaat
     1861 cgttttcagt tgagtggaat gatcggcaca aggttgcgcc atgcgagagt tccttcgcac
     1921 caaaggtttc aaaatgacgc cccagcggga gctggttttc cgggcgttct ttgaactcgg
     1981 ggaccacgtg acggtggacg agctgttcga gaaagtgagg cgccaggacc gctcgatcgg
     2041 gtacagcacc gtctggcgga acctgaaact gatctgccgg gtggggctgg cggaggaagt
     2101 gaatgtcggc gacggcgtca cgcggtacga ccgggtgacg cacgtgccgc acggccacct
     2161 gtactgccag gagtgccgca ggctgaccga gtttccggtg gaggaaatcg tggcggtgct
     2221 ggccggggtg gccgagcaga acgacttccg cgccgatagt ttcaaaatcg agatccacgg
     2281 gtactgcgag gaatgccgcg aacggcgccg gcgggcgctc ggttcgggcg gcggcgccgc
     2341 gccgccgaag tgacgggttc ggggacggga tgaggatgca gccatgacgt acttgagcag
     2401 aatgagtccc ggccagcgtg gacggatcgt aggattcgtc gacgacagcc cactggcgcg
     2461 ccgcctgctg gagctgggga ttatccccgg gcgcctgatc acctacctgc ggaacgcgcc
     2521 gctctccgat cccatgcagg tgcaggtcgg ttcgagctgc ctgaccctgc ggcaccagga
     2581 agcctcgctg gtggcagtgg agccggagga gtaaccccgc catttcccaa gggagaggcc
     2641 atccttcggg gaggtcgcat gccggcgccc gagattacaa agacccgtga aatcgccatt
     2701 tgcggcaatc cgaattgcgg caagacgacg atcttcaacg ccatcaccgg tctcgcccag
     2761 caggtgggca actaccctgg cgtgacggtc gagcggatcc gcgggcagtt cgcccatggc
     2821 ggccggcggt acgtgctggc cgacatcccg ggcacctatt ccctctcggc gttttctccc
     2881 gatgaataca tcgcctgcgc cgcactcacg ggcagcctgg atgcctcgcc ggcgcccgat
     2941 gcgatcatct gcgtgctcga cgggacccag ctcgagcggg ggctctattt cctgctccag
     3001 gtgctggaga tcggccgtcc gaccgtggcg gcggtcaaca tggtggatat cgccgcgaag
     3061 cgggggctga cgatcgacta cgccgcgctg gagcggctga ccggcgtgcc ggtcgtgccg
     3121 gtggtcggca gccgggggga aggcatcgcg cggctggttg cgttggccgc gcaggcggcg
     3181 gacgccccgc cgctcccgcc gcggcggcgc taccgggcgg aagtggagaa tctgatcgag
     3241 cgcctgcagg cgacgccggg caacggccac cggtcgcgcg ccgagtattt gcggatcgtg
     3301 tttgatgccg acggaccggc ggagcggcga ttcctggccg ccgccggcgg cgcgggtcgg
     3361 gcgttgctcg agcgcgggcg cagtctgatt cgcgagcggg ccggcacgct tgcggcggcc
     3421 gagaccgcac cgct
//
LOCUS       DAOOKS010000135         3422 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_33470_length_3422_cov_2.383724, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000135 DAOOKS010000000
VERSION     DAOOKS010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3422
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33470_length_3422_cov_2.383724"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="PK112_03075"
     CDS             <1..730
                     /locus_tag="PK112_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
                     /protein_id="HPC11064.1"
                     /translation="VVVPVFAMNVAPLQGFSFGVTFNLQHARMADINLRGTITERVGA
                     EYVALAIDNGEFSQTPPTRGEGWATFAVVLDVEPPFEGQTIPAGNGQRIANLVYDLQL
                     PPNPARSMRVPFVDGLGKPPVPVVYVVKGLDVRPAKRNGIIQLMWPPLFIRGDANDNG
                     SMTIADPIYLLNYLFGHGPVPPCLDAADANDDGLLNLSDAIAILAYLFRQGVIPPPSP
                     PGPPGYDPTPDDLDCARAEQGPRV"
     gene            complement(812..3238)
                     /locus_tag="PK112_03080"
     CDS             complement(812..3238)
                     /locus_tag="PK112_03080"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HPC11065.1"
                     /translation="MQSTRVSALVTAVFLCAFAAHGAPVPLPEAPAYFFTADSFPALA
                     AEGAAIAERNYVFWAWAREDSSVTLTAGGEEYFTDRPDPARAAPDRWTWRRLGTAVFA
                     GGRIALTADREGGAAALCLATDRSFEPAASWALMRSRPFDPQAAPDGRARECRHINLA
                     YPTYTFPSAEAWRARAAHLRDHILASCGLLPPPEKMPLAPVIFHRLEREGYTVEKAYF
                     ESWPGFYVTGNLYRPRGRQGPFPGVLSPHGHWGRGRFADEGEPAGGVPMRAIALARLG
                     CVVFTYDMVGYGDSKRQIGHSFASEQNELWGLSLMKLQLWNAIRGLDFLASLPDVDPE
                     RLACTGASGGGTQTFILMAVDERVKVAAPVCMVSAFMQGGCECENAPLLRLDTINPEI
                     AALMAPRPLILVSASGDWTSKTPSVELPMVRSVYELLGAADRLAGAHFEAPHNYNRDS
                     REAVYNFFLKWLLGAAPATPYKEPPYTLEKLEDVSVWHGRELPPGALDRPGLEALLRE
                     EAKARLRNHLPKDAAALARFRETIGAYYRHAVYIRGRTEGGPSAAEFLGMTHLSNATA
                     AAGTACLRLCIARDDQKLPALFFSAPPPDAGRATRIGAVLVIHPDGKAALIDAARGRP
                     GPLLEALLGRNLPVLAIDTFLTGEFHSAYGPTPRARADKMFTTYNRTTLVERVRDIAA
                     AAAFLEAKVADRIAVIGLGRAGKWVALAAPYLPDRALIIADLKDYASESETAWQGDDF
                     APHILGAGGLETACALAAPRPLICFSAGPAFRGAWILAAYAAAGATDRLSIMSHNLTP
                     EELAGLAAED"
BASE COUNT          524 a         1136 c         1254 g          508 t
ORIGIN      
        1 cgtggtcgtg cccgtattcg cgatgaacgt tgcgccgctt caaggcttct ccttcggcgt
       61 gaccttcaac ctgcagcatg cgcgcatggc ggatatcaat ctccggggga cgatcacgga
      121 gcgagtcggc gccgaatacg tcgcgctcgc catcgacaac ggtgaatttt cccagactcc
      181 gcccacccgc ggcgagggct gggccacctt cgcggtggtt ctggatgtcg aaccgccctt
      241 cgaggggcag acgattccgg cgggaaacgg ccagcgcatc gccaatctgg tctacgacct
      301 ccagttgccg ccgaaccccg cgcggagcat gagggtgcct tttgtcgacg gtctcggcaa
      361 gccgcccgtt cccgtcgtct acgtcgtcaa ggggctcgac gtgcggccgg ccaagcggaa
      421 cggcatcatc cagctcatgt ggccgcccct gttcatccgc ggcgatgcca acgacaacgg
      481 cagcatgacg atcgccgatc cgatctatct gctgaactac ctcttcggac acggtcccgt
      541 gccgccttgc cttgatgcgg ccgatgccaa cgatgacggt ctcctcaacc tgtccgacgc
      601 gatcgcgatt ctggcctatc tcttccgcca gggcgtgatt ccgccgccct ccccgcccgg
      661 accgccgggg tacgatccga cgcccgacga tctggactgt gcccgggcgg agcaggggcc
      721 tcgggtttag aagcgcgagg atgccggcgg cgttcttccc ggagcgtccg ggaagacgcc
      781 gccggctgtc tccgcctgcc gagccttcgg ctcagtcttc cgccgcgagc ccggcgagtt
      841 cctccggcgt caggttgtgg ctcatgatgc tcaaacggtc ggtggcgccg gcggcggcgt
      901 aagccgctag gatccaggca ccgcggaacg cggggcccgc actgaagcag atgagcgggc
      961 gcggcgccgc cagcgcgcat gcggtttcca agccccccgc gccgaggatg tgcggagcga
     1021 agtcatcgcc ctgccaggcg gtctcgctct cggaggcgta gtccttgagg tccgcgatga
     1081 tcaacgcgcg gtcgggaagg tacggcgccg ccagcgcgac ccacttgccc gcgcggccca
     1141 ggccgatgac cgcgatgcgg tcggcgacct tggcctcgag aaaggcagcc gcggcggcga
     1201 tgtcgcggac gcgctccaca agcgtcgtcc ggttgtacgt cgtgaacatc ttgtcggcgc
     1261 gggcgcgcgg cgtgggcccg taggcggagt gaaactcgcc cgtcaggaag gtgtcgatgg
     1321 cgagaacggg caggtttctg cccagcaacg cttcgagcag cggtcccggc cggccgcgcg
     1381 ccgcgtcgat cagcgcggcc ttgccgtcgg gatggatgac gagcacggcg ccgatgcgcg
     1441 tcgcgcggcc cgcgtccggc ggcggggcgc tgaagaacag cgcgggcagt ttctgatcgt
     1501 cgcgggcgat gcacaggcgc aggcaggcgg ttcccgcggc ggccgtggcg ttcgagagat
     1561 gagtcatgcc gaggaactcc gcggcggacg gcccgccctc ggtgcgcccg cggatgtaga
     1621 cggcgtgccg gtagtacgcg ccgatcgtct cgcgaaagcg cgcgagcgcg gcggcatcct
     1681 tgggaaggtg gttgcgcagg cgcgccttgg cctcctcgcg gagcaacgct tccagtcccg
     1741 ggcggtcgag ggcgccgggc gggagctccc ggccgtgcca gacgctcaca tcctcgagct
     1801 tctccagcgt gtacggcggt tccttgtacg gcgtcgccgg cgcggcgccc agcagccact
     1861 tcaggaagaa gttgtagacg gcctcgcggg agtcgcggtt gtagttgtgg ggcgcctcga
     1921 agtgcgcgcc ggcgagccgg tcggccgcgc cgaggagttc gtagacgctg cgcaccatgg
     1981 gaagctcgac ggacggggtc ttcgaggtcc agtcgccgct cgcggagacg aggatgagcg
     2041 gccgcggcgc catgagcgcg gcgatctccg ggttgatggt gtcgaggcgc aggagcggcg
     2101 cgttctcgca ttcgcagccg ccctgcatga aggccgagac catgcacacg ggcgcggcga
     2161 ccttcacgcg ttcgtccacc gccatgagga tgaaggtctg cgtgccgcct cccgaggcgc
     2221 cggtgcaggc gagcctttca gggtcgacgt cggggagact cgcgaggaaa tccaggccgc
     2281 ggatcgcgtt ccagagctgg agcttcatca ggctcaggcc ccacagctcg ttctgctcgc
     2341 tcgcgaagga gtggccgatc tggcgcttgg aatctccgta gccgaccatg tcgtaggtga
     2401 agacgacgca gccgaggcgc gccaaggcga tggcgcgcat ggggacgccg cccgcgggtt
     2461 cgccctcgtc ggcgaagcgt ccccggcccc aatggccgtg cggcgagagc acgccgggaa
     2521 aaggaccctg ccgcccgcgc ggtcgataga ggttgccggt gacgtagaag ccgggccagg
     2581 actcgaagta ggccttctcg acggtgtagc cctcgcgctc gagccggtgg aagatgacgg
     2641 gcgccagcgg catcttttcc ggcggcggga ggagcccgca ggaggcgaga atgtggtccc
     2701 ggagatgcgc cgcgcgcgcg cgccaggcct cggcggaggg gaaggtgtac gtcgggtacg
     2761 ccaggttgat gtggcggcac tcgcgcgcgc ggccgtcggg ggcggcctgc gggtcgaagg
     2821 ggcgcgagcg catcagggcc cagcttgccg cgggttcgaa actcctgtcg gtcgcaaggc
     2881 acagggcggc cgctccgccc tcgcgatccg cggtaagcgc gattctccct ccggcgaaaa
     2941 ccgcggtccc gagccgccgc cacgtccagc ggtccggcgc cgcgcgggcg ggatccggcc
     3001 gatcggtgaa gtattcctcg ccgccggctg tgagcgtcac ggaggagtcc tcgcgcgccc
     3061 acgcccagaa gacatagtta cgttccgcga tggccgcgcc ctccgcggcg agggcgggaa
     3121 aggaatcggc tgtgaaaaag taggccgggg cctcgggaag gggaacgggc gcgccgtgcg
     3181 cggcgaaagc gcagagaaac acggccgtca ccagagcgga aacgcgcgta gactgcatgg
     3241 gtgcacctcc agcggcgaag ccgaatatca ccgcaattat cgttgacggg agaagacgtg
     3301 tacagcgtgc gcgccgccta ggccggcacg acgcgcgccc cttcggcggg acgcgctatg
     3361 aagcgcagct cctcgatggg gccggcaggg gcgcgcgggc ggtagaagcg ctcgtcgatc
     3421 ac
//
LOCUS       DAOOKS010000136         3403 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_33727_length_3403_cov_3.035544, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000136 DAOOKS010000000
VERSION     DAOOKS010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33727_length_3403_cov_3.035544"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1462..2526)
                     /locus_tag="PK112_03085"
     CDS             complement(1462..2526)
                     /locus_tag="PK112_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11066.1"
                     /translation="MMRQLLSCLALIILFFPPAAPSRGQSAPGNHVAAELKTTDEVLE
                     QAREILPAVSPVPVKVSYESAVNLQAQAHEEYRLGPDPGRWEKALLLTIQARAKAQEA
                     IAQSASLGGPTTPDETALQRRLERADELMAQAREALEPSADNSLQALYESARSKLDRA
                     HGLCGERQYRAALKLAGQVERTAERVLQAATRAARDDFNYDRCRENVRRYFEQAGEQI
                     AECESPSALLPMEQARQAMDMSEKQASEGDRAGALHALQTARELAAQALRECTGNDNL
                     HHRHKTLRQQADRLGEAIGAENTDAFAFLAQARTQLDLAQAALEQGDRQAVVAALKAA
                     QLSLDEARRKTLAGRRTVGG"
     gene            complement(2689..>3403)
                     /locus_tag="PK112_03090"
     CDS             complement(2689..>3403)
                     /locus_tag="PK112_03090"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPC11067.1"
                     /translation="ESELFGHEKGAFTGAVARKRGRFELAQGGTIFLDEIGEMSPGLQ
                     SKLLRVIEERQLVRVGGVDQVDIDVRVIAATNRDLRSMIEKGRFREDLYFRLNVFPIR
                     LPDLNERPEDIVPLAEHFLAEKGYPERTTPPEVAHLLESYDWPGNIRELRNVIERATI
                     LAGGEPLTPEDFSLPVDDSPLTVPASSMAAAGLPAAERQIIMDAIERARGNKTEAARL
                     LKISRRRLYSRMKVHGIKP"
BASE COUNT          636 a         1113 c         1020 g          634 t
ORIGIN      
        1 ccgggggtcg gaaccccgct gccccgagag cactaccccg aacacagacc tgcgcccgct
       61 tcgggtctcg acacgatcaa tctctcgagg gaaatacctg cgcgatggac acgctaccaa
      121 caaacagaaa aaaccgctgt ggtcgcgcta tcgcttatga caaccagaac ggctccgagc
      181 tctgttgatc aacgctaagt cttacttaac gttcaaaggt gccacagata caaaacagaa
      241 gcataattca tacacaactg tttccctaga ccgatcataa actacatcaa cacacaaaat
      301 tgtcaagaac tttcttcaac aatttacaca aatcactaaa tacgataggc tatagtaata
      361 aaaaccccca agtgcccatg tgtccctcag cgtcgccgga atagcccatc acccccaaaa
      421 gcgaagcctt cagggcgtct tgaagacctt ccagccaccg gctcgttatc atatccgacg
      481 ggtcccggtc aagacaaggg gtcggctacc ccggcgccac aggagcgcct cggcctctac
      541 tgggaaccag ttccgagggg acgtctcgtc gagcaaaaga cctctagact gctcgcacaa
      601 gaagcattga ttgtgaaata agtcacaaaa tccccttacc tgcgcaacac cagccgggca
      661 ttccgacagc cgcctgctca tctatccgga cgacccctgt gagatccttt cgagcgcccg
      721 gccaaagacg atcgtcagac cggctgaccg gacccgggcg ggggagaggt cgccaccgaa
      781 agtttcggcg ggggcggcgt ccaagtggct gtcaccctcc gttccgtacc ctcgcccgtc
      841 ggcccggtgg cctccccagg atcgattcgt gggtggcgtc ggaaaactcg ccgatgatag
      901 tccgggatcg gttcttaccc ggctgagagc gtcctgagcg gccggccgag tccggtcatg
      961 ttgactcagc agaccgcccc caggggtttg aacaaatttc cgcgaacccc ctgctgatgg
     1021 ttcttccagc acactccgca acccgaagtg tgtcataatt gacacagcca aaagaccccg
     1081 ctgtcgtcct gctgtgacga ccagaaaagt gggggcgcga ccacccattc ccgcggctga
     1141 gccatcgggc ggatcagccg caatgcccgt gggtgatgaa ccatgggccg agaccgaact
     1201 caactgcacg ccggcgaggg gtgcagaaga cctcggaagc agccgaacgg gtcacggctg
     1261 ccggctcccg aagtgtgggc ggaactgccg attgctcggt accggaaagt aggctgggtt
     1321 acgccttaat cgtccagagt ccggtatcag gttgttcctg aattggggaa agcaccgccc
     1381 gaaggaaatg gccttgaccc gtgttcggcc ggctgaaagc tcgcgaaaga cttgcgccga
     1441 tccggtgtcc gctgcaatat atcatccccc cacagtccgc ctgcccgcga gggtttttcg
     1501 ccgggcctcg tccagactca actgggccgc tttgagggcg gccacgacgg cctggcggtc
     1561 accttgttcg agggcggcct gggccaaatc gagctgtgtc cgggcttggg cgagaaaggc
     1621 gaaggcatcg gtattctcgg ccccgatcgc ctcacccagc cggtccgcct gctggcgcag
     1681 agtcttgtgc cggtggtgga ggttgtcgtt accggtgcat tcccgcagcg cctgcgcggc
     1741 gagctcccgg gccgtctgca gtgcgtgcag agcgcccgcc cgatccccct cagaggcctg
     1801 tttctcgctc atatccatgg cctggcgggc ctgttccatg ggcagcaggg cggatggaga
     1861 ctcgcattcg gcgatctgct ccccggcctg ttcgaagtac cgccgcacgt tttcgcggca
     1921 acggtcgtag ttgaaatcat cgcgggccgc ccgcgtggcg gcctggagaa ctctctcggc
     1981 tgtccgctcc acctggccag ccagtttcag ggcggctcga tactgccgct cgccgcacag
     2041 cccgtgcgcc cggtcaagct tggagcgggc gctctcgtaa agggcctgca gcgaattgtc
     2101 agcgcttggt tcgagagcct cccgcgcctg cgccatcaac tcatcggccc gctccagtcg
     2161 ccgttgtagg gccgtctcgt ccggcgtcgt cggcccgccg agagacgcgc tttgggcgat
     2221 tgcctcctgg gctttcgcgc gggcctggat cgtgagcagc agcgccttct cccagcggcc
     2281 cggatcgggc cccagccggt attcctcgtg cgcctgcgcc tgcaggttca ccgccgattc
     2341 ataggacacc tttacgggca ccggtgaaac cgccggcaga atctcgcgtg cctgttcgag
     2401 aacttcatcg gtcgtcttca gttcggcggc gacatggttg cccggcgccg actgcccccg
     2461 tgagggggcg gcgggtggaa agaagaggat gatcagagca agacaactca ataactgacg
     2521 catcataggc gtttctccgg cgaaccgacg ctcatcctcc tatcaaaccc tgtgcccggc
     2581 ctgccttatc gaatcatcgc cttgtctcat aactgcttgc accgtgtccc gatattcccg
     2641 gtcacgcgcc gccgtaccgt acggtacagg tatgccccga atggcgcatc agggtttgat
     2701 tccgtggact ttcatgcggc tgtagagacg ccgccgggag atcttgagca gcctggcggc
     2761 ctcggtcttg ttgccgcggg cgcgttcgat cgcgtccatg attatctggc gctcggccgc
     2821 agggagtccc gcggcggcca ttgaggaggc cggcacggtc agcggcgaat catcgacagg
     2881 cagggagaag tcctccgggg tcagcggctc gccgccggcc aggatcgtgg cccgctcgat
     2941 cacgttgcgc agctcgcgga tgttgccggg ccaatcgtag gattccagca ggtgcgccac
     3001 ctccggcggg gtcgtccgct ccgggtaccc cttttcggcc aggaagtgtt ccgccagcgg
     3061 cacaatgtcc tcggggcgct cgttgaggtc gggcaggcgg atcgggaata cgttcagacg
     3121 gaagtagagg tcctcgcgga accgtccctt ctcaatcatg ctgcgcaggt cgcggttggt
     3181 ggcggcgatg acgcgcacgt cgatgtcgac ctggtcgacg ccgccgaccc ggacaagctg
     3241 ccgttcctcg ataacgcgga gcagtttcga ctgaaggccg ggggacatct cgccaatctc
     3301 gtccagaaaa atggtgcctc cctgggcgag ctcaaaccgt ccccgcttcc gggccaccgc
     3361 gcccgtgaac gccccctttt cgtgcccgaa gagctccgat tcg
//
LOCUS       DAOOKS010000137         3400 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_33775_length_3400_cov_3.292377, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000137 DAOOKS010000000
VERSION     DAOOKS010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33775_length_3400_cov_3.292377"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="PK112_03095"
     CDS             <1..667
                     /locus_tag="PK112_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HPC11068.1"
                     /translation="LLGALGWFIVTVLLGLALHMFGVYSLSVYLLSRLNPLYFFRRIR
                     TVMLTAFSTSSSNATLPTALAETQRNLGVPREISTFVLTVGATANQNGTALYEGVTVL
                     FLAQLSGVDLSLGQQILVAYMAILGGIGTAGVPSGSIPFIVIVLATIGVNPALIAVIL
                     GVDRILDMCRTTLNVTGDITAATFVARSEGYEFAPSGGNGDPAEPSAHGPEAAEEGGS
                     GSR"
     gene            667..1479
                     /locus_tag="PK112_03100"
     CDS             667..1479
                     /locus_tag="PK112_03100"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPC11069.1"
                     /translation="MTTAFDTAILGATGRPVRRLGLSASYWPGKKTIYEAVDCGVTLF
                     FAYGFDFQMIRALRNLFRTGRDRFVVVTGAYNLIWTHTDVRKSCEKRLRQLGTDYLDY
                     FLFLGVMKPREFPARVQEDLLRLREEGKVRGVGVSCHDRRFAGQLIAEGRVDTLMMRY
                     SAAHRGAETDIFPHVAAHHPTVISYTATRWRQLLRRPRGWPADRPIPTAPQCYRFVLS
                     HPCVDVALTAPSNAAQLRQNLLALEQGPLADEEMAFMREFGDVVHGRRPWFM"
     gene            complement(1574..2869)
                     /locus_tag="PK112_03105"
     CDS             complement(1574..2869)
                     /locus_tag="PK112_03105"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HPC11070.1"
                     /translation="MAKTLKETLADLIPKWREERTRLAKEHGDVKISDVTIAQAFGGM
                     RGVKGLVCDTSVVEPDQGLIIRGIPIGKLTDRLPEEIFWLLCTGNLPTADELKGLQKE
                     LRAAAEIPPYVLKVLKAMPKTSHPMAMLDTGILVMENESQFRKRYTDGMKKDVYWEPA
                     LEDSIRILGTITGIAAAVYRIKYFKGDLIELNKKEDWAADYVKMLGLPPHKGDTARMM
                     RLYLVLHCDHEGGNVSAFSAHTVGSALSDPYYALSAGLNGLAGPLHGLANQECLAWVL
                     ETIKKFGGAPSEEQLRKYAWETLNSGKVVPGYGHAVLRVTDPRFTAFIEFGKKYLAKD
                     PVFQTVNRVFNVVPDVLKEQGKAKDPWPNVDAASGSVLYPFGLTQFEYYTVLFSVSRA
                     MGILSQLVLNRALGTAITRPKSVSTEWLGKQVKKASVPA"
     gene            complement(3026..>3400)
                     /locus_tag="PK112_03110"
     CDS             complement(3026..>3400)
                     /locus_tag="PK112_03110"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HPC11071.1"
                     /translation="RVSEDVAVPNSKFPSLVAFVMEMNAASPLRINSYGHAGDGNLHV
                     NFISMTGSEADRAEIGRRVEQLMRRTIALGGTLSGEHGIGLAKRRFLSLEFDPATRAA
                     MQKFKDVFDPHDILGRGKIFEK"
BASE COUNT          571 a         1049 c         1121 g          659 t
ORIGIN      
        1 cctcctcggc gccctcggct ggttcatcgt caccgtgctc ctggggctgg ccctgcatat
       61 gttcggcgtg tactcgctgt cggtctacct gttgtcgcgg ctcaaccctc tgtacttctt
      121 ccggcggatc cgcacggtga tgctcaccgc cttctcgacc tcgtcctcga acgccaccct
      181 gcccaccgcg ctggccgaga cgcagcggaa cctcggcgtg ccgcgcgaaa tcagcacctt
      241 tgtgctgact gtcggggcga ccgccaatca gaacggaacg gcgttgtacg aaggagtcac
      301 ggtgctgttt ttggcgcagc tctccggcgt cgatctgagc ctcgggcagc agattctcgt
      361 cgcttatatg gccatcctcg gcgggatcgg cacggccggc gtgccgtcgg gatcgatccc
      421 cttcatcgtc atcgtcctgg cgacgatcgg cgtgaacccg gccctcattg ccgtgatcct
      481 cggcgtcgat cggattctcg acatgtgtcg gaccacgctg aatgtgaccg gcgatatcac
      541 ggcggcgacg ttcgtggcgc gatcggaggg gtacgagttc gcgccgagcg ggggcaatgg
      601 ggatcccgcg gagccgtcgg ctcacggacc cgaggcggcc gaggagggag ggtcaggcag
      661 ccggtgatga cgaccgcgtt tgacaccgcc atcctgggag ctaccgggag accggtgcgg
      721 cggctggggc tctcggcgtc gtactggccg gggaaaaaaa cgatctacga ggcggtcgac
      781 tgcggagtaa ccctgttttt cgcttacgga ttcgatttcc aaatgatccg ggcgctgcgc
      841 aatctcttta gaaccggccg cgaccggttc gtcgtggtca ccggcgcgta caacctgatc
      901 tggacgcaca ccgacgtcag aaagtcgtgc gagaagcggc ttcggcagct cggcacggac
      961 tatctcgact acttcctttt cctgggcgtg atgaaacccc gcgagttccc cgcgcgggtt
     1021 caggaggatc tgctgcggct gagggaggag gggaaggtcc ggggagtggg ggtgtcgtgc
     1081 cacgatcggc gcttcgccgg gcagctcatc gcggagggga gggtggacac tctgatgatg
     1141 cgctacagcg cggcccaccg cggagcggag acggacatat tcccccatgt ggcggcccac
     1201 catccgaccg tgatcagcta cacggcgacg cgctggcggc aactgctgcg gcggcccaga
     1261 gggtggccgg cggaccggcc gattccgacc gcgccccagt gctaccggtt cgtgctgtcc
     1321 catccctgtg tggacgttgc gctgaccgcc ccctcgaatg ccgcgcagtt gcggcaaaac
     1381 ctgctcgccc tcgagcaggg gccgctggcg gacgaggaaa tggcgttcat gcgggaattc
     1441 ggcgacgttg tccacgggcg aaggccgtgg tttatgtgag gcgcccgccg cgtccgggca
     1501 aaatgaagcc ggcccgctcg tccgagcagg ccggcgtgtt ttcatggtct cgtgggaaga
     1561 gacgcccagc cgctcaggcc ggcacgctcg ccttcttcac ctgcttgccc agccactcgg
     1621 tgctgaccga cttcgggcgg gtgatagcgg tgcccagggc gcggttgagc acgagctggg
     1681 agaggatccc catggcgcgc gacacgctga agagcaccgt gtagtactcg aattgcgtca
     1741 ggccgaacgg gtagagaacc gaccccgaag cggcatcgac attcggccac ggatccttcg
     1801 ctttcccctg ctccttgagc acgtcgggga ccacgttgaa gacgcggttg acggtctgga
     1861 agaccggatc cttggcgagg tacttcttgc cgaactcgat gaacgcggtg aagcggggat
     1921 cggtgacgcg caggacggcg tggccgtagc cgggaaccac tttgccgctg ttgagcgtct
     1981 cccacgcgta cttgcgcagc tgctcctcgc tcggggcgcc gccgaatttc ttgatggtct
     2041 caagcaccca cgcgaggcac tcctggttcg ccaagccgtg gagagggccg gccagaccgt
     2101 tgagacccgc gctgagggcg tagtagggat cggagagcgc gctgccgacg gtgtgggcgg
     2161 agaaggcgga gacgttgccg ccctcgtggt cgcagtggag cacgaggtac agccgcatca
     2221 tgcgcgcggt atcccccttg tgcggcggca aaccgagcat cttgacatag tcggccgccc
     2281 agtcctcttt cttattgagc tcgatgaggt cgcccttgaa atacttgatg cggtagaccg
     2341 cggccgcgat cccggtgatc gtgccgagga tgcggatcga gtcctcgagt gccggctccc
     2401 agtagacgtc cttcttcatc ccatcggtgt agcgcttgcg gaactgcgac tcgttctcca
     2461 tgacgagaat gcccgtgtcg agcatggcca tcgggtgcga cgtcttgggc atcgccttga
     2521 gaactttgag gacgtacggc ggaatctccg cggccgcgcg gagttccttc tgcagaccct
     2581 tgagctcgtc cgcggtcggg agattgccgg tgcagaggag ccagaagatc tcttcgggca
     2641 gacggtcggt cagcttgccg atcgggatgc cgcgaatgat gaggccctgg tcgggttcca
     2701 cgaccgaggt gtcgcacacc agacccttga cgccgcgcat gccgccgaac gcctgtgcga
     2761 ttgtgacgtc ggaaatcttg acatcgccgt gctccttggc gagtctcgtg cgctcctcgc
     2821 gccacttggg aatgagatcg gcgagagttt ccttcaatgt tttggccatc gattgctcct
     2881 tttaattctt gaaggtttaa cgcgagagga tattaccgtt ttgtcaggtc cccaatatgt
     2941 gaaaggtttc acattaacgg gcggtagtgt aacacctggc tgcaggcttg tcaagtgggg
     3001 tcgggcaaaa agcccgaaag gggggtcatt tttcgaagat tttgccgcgc ccgagaatgt
     3061 catgcgggtc gaaaacatcc ttgaacttct gcatcgccgc ccgcgtggcc ggatcgaatt
     3121 caagactgag aaagcggcgt tttgccagtc cgatcccgtg ctcgcccgac aacgtcccgc
     3181 ccaacgcgat cgtccggcgc atcaattgct caacccggcg cccgatctcg gcccggtccg
     3241 cctcagagcc ggtcatgctg atgaagttga catgcaggtt gccgtcgccc gcgtggccat
     3301 acgagttgat ccgcaggggg ctggccgcgt tcatctccat cacaaacgcc accagcgacg
     3361 ggaatttgga gttcggaacc gccacgtctt cggacacgcg
//
LOCUS       DAOOKS010000138         3395 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_33833_length_3395_cov_3.320958, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000138 DAOOKS010000000
VERSION     DAOOKS010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33833_length_3395_cov_3.320958"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /gene="rplQ"
                     /locus_tag="PK112_03115"
     CDS             complement(<1..394)
                     /gene="rplQ"
                     /locus_tag="PK112_03115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HPC11072.1"
                     /translation="MGHQDKIAKLGRTKSHREAMLRNMAMSLFEHRVIKTTDAKAKAL
                     RPLVDRLITNAKKGTLASKREVARTVHQKRIFKKLFAEIVPQFEDRASGFTRVVKLGV
                     RRGDGAALSVVELLTERPKEEPVKKAKKG"
     gene            complement(414..1409)
                     /locus_tag="PK112_03120"
     CDS             complement(414..1409)
                     /locus_tag="PK112_03120"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003513.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HPC11073.1"
                     /translation="MKWKPLTMPKEVVNDQSSANENYARFIVEPLERGYGTTLGNSLR
                     RVLLSSIQGAAVVSMRIRNVLHEFSSVEGVYEDVSDIVLNVKQIRVRMHADEMRTLTL
                     RRHGKGKLTAGMFEQNPEVEILNPDQHICEITGDIDFEMEIDIDSGRSYNVAEQNKRA
                     DAPVGTIFVDSLFSPVIKVAYQVENTRVGQKTDFDRLIMEITTDGSITPEDALSYGAK
                     LLKDHLQLFIHLDEEVMVEEEPQEDEETVRVRNLLRTRVDELELSVRSSNCLRAANIQ
                     TIRDLVTKTEPEMLKYRNFGRKSLNEISTLLEEMGLSFGMDVSKYDETQKQNVNA"
     gene            complement(1432..2061)
                     /gene="rpsD"
                     /locus_tag="PK112_03125"
     CDS             complement(1432..2061)
                     /gene="rpsD"
                     /locus_tag="PK112_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HPC11074.1"
                     /translation="MARYRDSKCKLCRREGEKLFLKGARCLTDKCAIERRPFPPGQHG
                     QSRRRKISPYGLQLREKQKIRRLYGLLETQFHNYFEKAAQKKGVTGELLLQSLETRLD
                     NLVYRLGFAPSRNAARQLVRHRHVLVDGGIVDIPAYSVRPGQVIRIKEKSRNLDLIHT
                     SLKEVGRAGDLPWLRLNKASLEGELLEVPKRSDIPLTVNESLVVELYSK"
     gene            complement(2070..2489)
                     /gene="rpsK"
                     /locus_tag="PK112_03130"
     CDS             complement(2070..2489)
                     /gene="rpsK"
                     /locus_tag="PK112_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019908251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HPC11075.1"
                     /translation="MAEPKKKGRPREKSRKVDTNGVAHIKATFNNTIITLTDAQGRAI
                     SWGTAGKVGFKGSKKSTPFAAQQAAASAVREAMDQGLRRVEVWVKGPGSGREAAIRSL
                     SAAGLEITLIRDVTPIPHNGCRPPSGAACEQMKRGRK"
     gene            complement(2511..2897)
                     /gene="rpsM"
                     /locus_tag="PK112_03135"
     CDS             complement(2511..2897)
                     /gene="rpsM"
                     /locus_tag="PK112_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HPC11076.1"
                     /translation="MARIAGVDLPKDKRIEVGLTYIYGIGPHRARLILAKTGVNPDTR
                     VNQLTDEDVTKLRQVIENEYSVEGSLRGEIQMNIKRLIDIGSYRGLRHRRGLPVRGQR
                     TKTNARTRKGPRRSIGGLKKKPVGKK"
     gene            complement(2936..3052)
                     /gene="rpmJ"
                     /locus_tag="PK112_03140"
     CDS             complement(2936..3052)
                     /gene="rpmJ"
                     /locus_tag="PK112_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HPC11077.1"
                     /translation="MKVKSSVKKRCEYCKIIRRHGVIRVICKRDPSHKQRQG"
     gene            complement(3081..3299)
                     /gene="infA"
                     /locus_tag="PK112_03145"
     CDS             complement(3081..3299)
                     /gene="infA"
                     /locus_tag="PK112_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HPC11078.1"
                     /translation="MAKEETITVEGRVIEPLPNATFKVELDNGHVVLAHISGKMRMHF
                     IKILPGDKVTLELSPYDLTRGRITYRYK"
     gene            complement(3308..>3395)
                     /locus_tag="PK112_03150"
     CDS             complement(3308..>3395)
                     /locus_tag="PK112_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019632597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HPC11079.1"
                     /translation="VTADGRPSAHFEHTVAVGPDGPRILTAA"
BASE COUNT          536 a         1140 c          967 g          752 t
ORIGIN      
        1 tgcccttctt cgccttcttc accggctctt ctttgggccg ttcggtgagc agctcgacca
       61 ccgacagggc cgccccgtcg ccgcgccgca cacccagttt gaccacccgg gtgaaccctg
      121 acgcgcggtc ttcgaactgc ggcacgatct cagcgaagag cttcttgaag attcgcttct
      181 gatggaccgt gcgagccacc tcacgcttcg acgccagcgt ccccttcttg gcattggtga
      241 tcagccggtc gaccagcggc cgcagcgcct tcgccttggc atcggtggtc ttgatcaccc
      301 ggtgctcaaa gagcgacatc gccatattgc ggagcatggc ctcgcggtgc gatttggttc
      361 gccccagttt tgctatttta tcctgatgtc ccatcgcaaa tcttcacttc agcttaggca
      421 ttcacgttct gtttctgcgt ctcgtcgtac ttcgacacgt ccatcccgaa cgacaacccc
      481 atctcctcca ggagcgtgga gatttcgttc agcgacttcc gcccaaaatt ccggtacttc
      541 agcatctccg gctcggtctt ggtgaccagg tcccggatcg tctggatgtt ggccgcccgc
      601 aggcagttcg acgaccggac cgacagctcc agctcgtcca cccgcgtccg cagcagattg
      661 cgcacccgca ccgtctcctc gtcctcctgc ggctcctcct ccaccatcac ctcttcgtcg
      721 aggtggatga acagctggag gtggtccttc agcagcttgg ccccatacga cagcgcatcc
      781 tcgggcgtga tcgaaccgtc ggtcgtgatc tccatgatca ggcggtcgaa atcggtcttc
      841 tgcccgaccc gggtgttctc gacctggtag gccaccttga tcaccggcga gaagagggaa
      901 tcgacgaaga tcgtcccaac cggggcgtcg gcccgcttat tctgctcggc gacgttgtag
      961 ctgcggccgg agtcgatgtc gatctccatt tcgaagtcga tatcgccggt gatttcgcaa
     1021 atgtgctggt cggggttgag gatctcgact tccgggttct gctcgaacat cccggccgtg
     1081 agtttcccct tcccgtggcg gcgaagcgtg agcgtgcgca tctcatcggc atgcatccgc
     1141 acccggatct gcttgacgtt gaggacgatg tcgctcacat cctcatagac gccctcaacg
     1201 ctggagaact cgtgcaggac gttccgtatc cgcatcgaca cgaccgccgc gccctgaatc
     1261 gaggagagca gcacccgccg gagactgttc cccagcgtcg tgccgtaccc gcgctccagc
     1321 ggctcgacga taaaccgtgc gtagttctcg ttggccgagg actggtcgtt aacgacctct
     1381 ttcggcatgg tcaatggctt ccacttcata gcttgctcgg ccctcccaac attacttgga
     1441 gtacagctct acgaccaatg actcgttcac cgtcagcgga atgtccgacc gcttcggcac
     1501 ttcgagcagt tcgccttcga gactggcctt gttcagccgc agccagggca ggtcgccggc
     1561 ccggcccact tccttcaggg aggtatgaat caagtcgaga ttccgcgact tttccttaat
     1621 ccggatgacc tggccgggac ggacgctgta ggcgggaatg tcgacaatgc cgccgtcgac
     1681 caggacgtgc cgatggcgga cgagctggcg ggcggcgttg cgcgacggtg cgaagccgag
     1741 ccggtagacg aggttgtcca ggcgcgtctc gagcgactgc agaagcagtt cgccggtgac
     1801 gcccttcttc tgggccgcct tctcgaagta gttgtggaac tgggtttcga gcagtccgta
     1861 cagccgccga atcttctgtt tttcgcgcag ctggaggccg tacggcgaaa tcttccggcg
     1921 gcggctctgg ccgtgctgcc ccggcggaaa aggccgccgc tcgatggcgc acttgtccgt
     1981 cagacagcgc gcccccttga ggaacagctt ctcaccttcc cggcgacaca gtttgcattt
     2041 cgagtcacga taccgtgcca ttggctctcc tatttccgac cacgtttcat ttgctcacac
     2101 gcggcgccgc ttggcgggcg gcagccgtta tggggaatcg gggtcacatc gcggatgagg
     2161 gtgatctcga gaccggccgc cgagagcgag cggatagcgg cttcgcggcc cgatcccggc
     2221 cccttcaccc agacctcgac ccgccgcagc ccctggtcca tggcctcgcg cacagcgctg
     2281 gccgcggcct gctgggccgc aaacggcgtc gacttcttcg accccttgaa ccccaccttg
     2341 ccggccgtcc cccacgagat cgcccgaccc tgcgcatcgg tcagcgtgat aatcgtattg
     2401 ttgaaagtgg ccttgatgtg ggccacgccg ttcgtatcaa ccttgcggga cttttctctc
     2461 ggtcgtccct tctttttcgg ttccgccacg actgttctcc tccgggtcgc ttacttcttg
     2521 ccaacaggtt ttttcttgag cccgccgatc gagcgccgcg gacccttgcg cgtccgggcg
     2581 ttcgtcttgg tccgctgtcc ccgcacgggg aggccgcgcc ggtggcgcag gccgcggtag
     2641 ctgccgatat cgatcagccg cttgatgttc atctggatct cgccgcgcag cgacccctcg
     2701 acgctgtact cgttctcgat gacctgccgc agcttcgtca cgtcctcgtc ggtcagctgg
     2761 ttgacccggg tgtcgggatt cactcccgtc ttggcgagaa tcagcctcgc ccggtgcggg
     2821 ccgatcccgt agatgtaggt cagcccgacc tcgatccgct tgtccttggg aagatcaact
     2881 ccggctatac gtgccaatgg tttttcctca cttctctatc gcacacacac aattctcaac
     2941 cctggcgctg cttgtggctg gggtcgcgct tgcagatgac ccggatcacg ccatggcgcc
     3001 gaatgatctt gcagtactcg cagcgcttct tgaccgacga cttgactttc ataccgacca
     3061 ccgatcccgt ctggggttac tcatttatag cgatacgtga tccggccgcg cgtgagatcg
     3121 tagggcgaca gttccagcgt caccttgtcg ccggggagga tcttgatgaa atgcatccgc
     3181 atcttccccg aaatgtgcgc cagcaccacg tgtccgttgt ccaactccac tttgaaagtg
     3241 gcgttcggca gcggctcgat cacccggcct tccaccgtta tcgtctcttc tttcgccata
     3301 cgctccttta ggccgccgtg aggatccgcg ggccgtccgg gcccaccgcg acggtgtgtt
     3361 cgaaatgggc cgacggacgt ccgtccgccg tcacc
//
LOCUS       DAOOKS010000139         3376 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_34092_length_3376_cov_2.282144, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000139 DAOOKS010000000
VERSION     DAOOKS010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34092_length_3376_cov_2.282144"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1900
                     /locus_tag="PK112_03155"
     CDS             <1..1900
                     /locus_tag="PK112_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11080.1"
                     /translation="GFPSLSCDTAAFPLAANWTTFWPETSPPSVATFTLSSDPSADAV
                     ATHVFRSLAPATSLQEGGIVGVKSRVAFFDDTSDVHLFGFHLYHMDTLDARALLAALR
                     TPNSAPALALTGERGYERKGVVPQWGNPGTAFEFRVEFSDPDGDLPLPGYPRVHLYSA
                     LTGGPAVGSPFPMTAVSGARSAGQVYAAVVQDLPPDPSYTYWFEARDEHGAAAAGAVT
                     AAHRFYGPVVTDPGGAVRWVAVTGNDTTGTGDEGAPLASVQRAADLANNGDTIRIGDG
                     VYPGAFLSFWGKQLVIESVHGAAATVIDGGLSPNVIASTKIGVPLAGFEDSTFVLRDL
                     TLRNFGNDTLDGRVLDLNGSSPSIRDCRFEATRGRSAGAVVCRGGSPVFEGCAFIANR
                     ADSAGGAVWLDSCEIRFVGCTFAGNAAPLGSAVWASASHVTLERCILAYGQGSPPVAF
                     DPAHPGAVTVSCTDLFGNAGGDWSSCLAGLGTGDNLTRNPQFCDTTGGDYHLFVHSPC
                     RAEMNNCAALIGAYAVDEACAGCCALRGDLTGEGSVTVADITALIRQVFRGGAEPGCP
                     EHSNVNGGLGVSLIGPVSVVDITYLVNYVFRGGSAPPACGFPGAPEEVVPDFGAVGGA
                     APVPSPRP"
     gene            complement(1988..>3376)
                     /locus_tag="PK112_03160"
     CDS             complement(1988..>3376)
                     /locus_tag="PK112_03160"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HPC11081.1"
                     /translation="TQYIQLSNEGYGALERGQQDSALAAFRKQAEVIPEGRWGLYNLA
                     CAYSRMGNADAALAYLDTAVTRGWTVVDHLENDPDLAPLRDDPRFAAILDKARQLDTE
                     KMGMLAAGLPRDVTPPQGITDSAALVAWFDGQQEILRVNSQVWHPWQTAAANLDLEAK
                     RLAATEALLGDRFDYDLERIISLGRMNSPYDEKWGAVSQTIMKEVDAYLATSPNAESA
                     SEACYRGVAAAIMEHGPAATGTPEGQAALAKAESYYGRMDSSYRRYGGAEGWILAARL
                     GAAGEARETLYPQIKDFAARNAADENAMAVAKTLFPADMVKASWPIPLTATDIDGKPV
                     SLEDYRGKVLLLDFWATWCGPCRAELPYLKAAYEKYRSQGFDILSISLDYPNQTTQDA
                     YRAWITEAGMPWRHVYDEQNWTGGITQAFAVGSIPSPFLIGKDGTLIAMHDTCRGPAL
                     DTLIQKALAMQM"
BASE COUNT          469 a         1162 c         1155 g          590 t
ORIGIN      
        1 cggtttcccg tcgctctcct gcgacaccgc cgccttcccg ctcgctgcga actggaccac
       61 gttctggccg gaaaccagtc ccccctcggt ggcgaccttt acgctttcgt cggatccctc
      121 ggccgatgcc gtcgccacgc acgtctttcg gtcgctcgcg ccggccacct ccctgcagga
      181 gggagggatc gtcggcgtca agagtcgggt ggctttcttc gatgacacgt ccgacgtgca
      241 cctcttcggt ttccatttgt accacatgga cacgctcgat gcccgggcac tgctggcggc
      301 cctgcggacg cccaattcgg ccccggccct cgccctgacc ggcgagcgcg gctacgagcg
      361 gaaaggcgtg gtcccgcagt gggggaatcc cggcaccgcc ttcgagttcc gcgtcgagtt
      421 cagcgatccc gacggcgacc tgccgctgcc cggctacccg cgggtgcacc tttacagcgc
      481 cctcaccggg ggaccggcgg tcggaagtcc gttcccgatg acggcggtgt cgggcgcgcg
      541 ctcggcgggc caagtgtacg cggcggtggt gcaagacctc ccgcccgatc cctcgtacac
      601 gtactggttc gaggcgcgcg atgagcacgg cgcggccgcc gccggcgccg tcaccgcggc
      661 ccaccggttt tacggtccgg tcgtcacgga tccgggcgga gcggtccggt gggtggccgt
      721 aaccggcaac gacaccaccg gcacgggcga cgagggcgcg ccgctggcct ccgtccagcg
      781 cgccgccgat ctcgccaaca acggcgacac catccgcatc ggcgacggcg tctatcccgg
      841 cgccttcctc agtttctggg gaaaacagct ggtgatcgag tcggttcacg gcgccgcggc
      901 gaccgtcatc gacggcgggc tgtcacccaa tgtgatcgcc tcgaccaaga tcggagtgcc
      961 gctggcgggt tttgaggaca gcacgtttgt gctgcgcgat ctgactctgc gcaacttcgg
     1021 aaatgatacg ctcgacggcc gggtgctgga cctgaacggg agcagtccgt cgatccgcga
     1081 ctgccggttt gaggcgacgc gggggcggtc cgccggggcg gtggtctgtc ggggcgggag
     1141 tcccgtcttc gaaggctgtg cctttatcgc caaccgggcc gactcggccg gaggggcggt
     1201 gtggctcgac tcgtgtgaga tccggtttgt cggctgcacg tttgccggca acgccgcccc
     1261 gctgggttcg gcggtgtggg cttccgcgtc gcacgtcacg ctcgagcgct gcatcctcgc
     1321 ctatggccag gggagcccgc cggtggcctt cgacccggcg caccccggcg ccgtaacggt
     1381 ctcctgcacc gatctcttcg gcaacgcggg gggcgactgg agcagctgcc tcgccgggct
     1441 gggaacaggc gacaacctga cccgcaaccc gcagttctgc gacacgaccg gcggcgacta
     1501 ccacctcttc gtgcactccc cctgccgggc ggaaatgaac aactgcgccg cgctgatcgg
     1561 ggcttatgcg gtcgatgagg cctgcgccgg atgctgcgcg ctgcgggggg atctcaccgg
     1621 cgagggttcg gtgacggtcg ccgacatcac cgccctcatc cgccaggtct tccgcggcgg
     1681 ggcggagccg ggttgcccgg agcactccaa cgtcaacgga ggcctggggg tcagcctgat
     1741 cggccccgtg agtgtggtag atatcacgta cctggtcaac tatgtcttcc gaggcggatc
     1801 ggcgcctccc gcctgcggtt tcccgggcgc gcccgaagaa gtcgttcctg attttggggc
     1861 ggtcggcggc gccgctccgg ttccctcgcc gcggccgtag ctcaagcggt gaagatgtca
     1921 taagagaagg cggcctccgc gcgggggccg ccttcgtgct ctgcggaccg gtcacacgca
     1981 agccgggcta catctgcatt gccagcgctt tctgaatgag cgtatcgagc gccgggccgc
     2041 ggcaggtgtc atgcatggcg atcagcgtcc cgtccttgcc gatcaaaaaa ggcgagggga
     2101 tcgagccgac cgcgaaggcc tgggtgatgc cgccggtcca gttctgttcg tcgtagacgt
     2161 ggcgccaggg catcccggcc tcggtgatcc acgcccggta ggcatcctgg gtcgtctggt
     2221 tgggatagtc gagcgagatc gacaggatgt cgaatccctg ggatctgtat ttctcgtatg
     2281 cggctttcag gtagggcagt tccgcgcggc acgggccgca ccaggtggcc cagaaatcca
     2341 gcaggagcac tttgcccctg tagtcctcga gcgacaccgg cttgccgtcg atatcggtgg
     2401 ccgtcagcgg gatcggccag ctcgccttca ccatgtcggc gggaaagagc gtcttggcga
     2461 ccgccatcgc attctcatcg gcggcgttgc gggcggcgaa atctttgatc tgcggataga
     2521 gcgtctcgcg cgcctccccg gcggcgccga ggcgggccgc caggatccac ccctccgccc
     2581 cgccatagcg gcggtaggac gagtccatcc ggccgtagta gctctccgct ttggccagcg
     2641 ccgcctgccc ctcgggcgtg ccggtcgcgg cgggaccgtg ctccatgatg gccgccgcca
     2701 ccccgcggta gcaggcctcc gaggccgatt ctgcgttcgg cgaggtcgcc aggtaagcgt
     2761 cgacttcctt catgatagtc tgcgagaccg ccccccactt ctcgtcgtag ggggagttca
     2821 tgcggcccag cgagatgatg cgctcgaggt cgtagtcgaa acggtcgccc aggagcgcct
     2881 cggtagcggc cagccgcttc gcctcgaggt caaggttggc cgcagcggtc tgccaggggt
     2941 gccagacctg gctgttgacg cggagaatct cctgctgccc gtcaaaccac gccaccagcg
     3001 ccgcggagtc ggtgattccc tgcggcggcg tcacgtcgcg cggcaggccc gccgccagca
     3061 tccccatctt ctccgtatcg agctggcggg ccttgtccag gatcgcggcg aagcgcgggt
     3121 cgtcccgcag cggagcgagg tccgggtcgt tttcgagatg atcgactacg gtccagccgc
     3181 gggtgacggc cgtgtcgaga taggccagcg ccgcgtcggc gttccccatc cggctgtagg
     3241 cgcaggcgag gttgtacagc ccccaccgtc cctcgggaat cacctccgcc tgtttccgga
     3301 acgccgccag cgcggaatcc tgttggcccc gctcgagggc gccgtacccc tcgttgctca
     3361 actggatgta ctgggt
//
LOCUS       DAOOKS010000140         3374 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_34113_length_3374_cov_3.358240, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000140 DAOOKS010000000
VERSION     DAOOKS010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34113_length_3374_cov_3.358240"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1784)
                     /locus_tag="PK112_03165"
     CDS             complement(<1..1784)
                     /locus_tag="PK112_03165"
                     /inference="COORDINATES: protein motif:HMM:NF013506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HPC11082.1"
                     /translation="MMRFHRWTALLAAAAAIPLGAKAQEVPFPDDVFYYAPAATAFGP
                     EAVWVNPAVLGPYKPATVAFMGDLAEGSVLKNWGTVITRRGFGLAYRRIDSDAGEDFK
                     EYALAGGLSLGSSLSVGGSYRYFPSGPSPYDHRHYWNIGLTSRGNSPFAVGAVWSNLN
                     RSELEGEDTRVIQRYSLGYRPQGATVTLAVDALSSAAAAEDDWTYIYHAQVIPATGLS
                     IEAFVDSDGNFEIGARANLLEYFVGTQSHFDSDAGHRFSHLVVGATDSRQPSLIRGSA
                     RRLSVSVSGAADENPVQTFFGARRPAFADLVLGIYRAAGDPGVGEMVLHLDNLRLTLA
                     QAQEMREAMAWFRAQGKSIVSHLTGPNNIAYYVACAGDSILIPPVSDLDLIGLRAELT
                     FFAGTMEKLGVAADMVRIGDYKTAPEAYTMRTPSEANREMTNRLLDDLYAQFVGGLAE
                     GRGMDPEAMRGLIDSGPYTSTEALDLGLVDGLTYRDRLVPDKFLSPLPEISFRAYRAD
                     TLMNDGWPPLPEIAVVYADGEIQPEGAADPSPLSGRGREVTPALMESAFGQALANPQV
                     QGVVFRIDSPGGYALAGNEIFHRAELAAR"
     gene            complement(1781..2860)
                     /locus_tag="PK112_03170"
     CDS             complement(1781..2860)
                     /locus_tag="PK112_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11083.1"
                     /translation="MTRSHSRPARRAACALLAAAVVGIGPARAQEIHDAGEGRFATRP
                     LTVAAPLAPGEKLLVRSAATLAGKITIRATETDRAVVVYTKQAVTDSRSRAVDYIDLI
                     AVDIGRVADGARLQLRAPNPAPWSGAESGSVDAEIRVPENVFVDIDALYFDVTAEGPF
                     SGVVNPSSLGRLEVYRVRGPVDLGTANRRVILEDVAGDIAVRTSNSTLTASDIESREH
                     QAVFRNDGGEIRIEGLTGEVSIRNDFGLIDVTDFRPLGRRNVIRGQSAPITLEIADLG
                     ESQLVVSNRFEDVDITAAADLSAVMSLAVGESGRIEVSGLRFQTDLVQRDRLGLVVGG
                     GQGFISSSISGSGNIYIRGRGQGEE"
     gene            complement(2844..>3374)
                     /locus_tag="PK112_03175"
     CDS             complement(2844..>3374)
                     /locus_tag="PK112_03175"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPC11084.1"
                     /translation="PAPQGIVGVFTIPRIDPAELCARRHRTILVCENVADPGNLGMLL
                     RSALAFGFRLVVTVGTTAEPFAPKVVRSSAGAVFGVRIAAADCDTLARLVASEGITLL
                     AAGRGGTQTLSESLRPDPAARYALAVGSEAEGLSDRMRALAACTVRVAHAPEVESLNA
                     AVAGSILMKGIYDSLS"
BASE COUNT          544 a         1124 c         1205 g          501 t
ORIGIN      
        1 cgggcggcca gctcggcccg atggaagatc tcgttgccgg ctagggcgta gccgccggga
       61 gagtcgatgc ggaacacgac tccctgcacc tgcgggttgg cgagcgcctg cccgaaggcg
      121 ctctccatga gcgccggggt cacctcgcgg ccgcggcccg agagcgggct gggatccgca
      181 gccccctcgg gctggatttc cccgtcggcg tacacgacgg cgatttcggg cagcggcggc
      241 caaccgtcgt tcatcagcgt gtcggcccgg taggcgcgga aactgatttc cggcagcgga
      301 ctgagaaact tgtcggggac caagcggtcc cgataggtga gcccgtcgac caggccgagg
      361 tcgagggcct cagtcgaggt gtagggcccg ctgtcgatca ggccgcgcat ggcctccggg
      421 tccatgccgc gcccctccgc caggccgccc acgaactggg cgtagaggtc atcgagcagc
      481 cggttggtca tctcccggtt tgcctccgac ggcgtccgca tcgtgtacgc ctccggggcg
      541 gtcttgtagt cgccgatacg taccatgtcc gcggccacgc cgagcttctc catcgtcccg
      601 gcaaagaaag tcagttcggc ccggaggccg atgaggtcga ggtcgctgac cggcgggatg
      661 aggatcgagt cgccggcgca ggcgacgtag taggcgatgt tgttcggtcc ggtgaggtgg
      721 ctgacgatgc ttttcccctg cgcccggaac cacgccatgg cctcgcgcat ttcctgggcc
      781 tgggcgaggg tgaggcggag gttgtccaga tggagcacca tctcccccac gcccgggtcg
      841 ccggccgccc ggtagatgcc gagcacgaga tcggcgaagg cgggacgccg cgcgccgaag
      901 aatgtctgca ccgggttctc gtcggcagcc ccgctcacgg acacggacag acggcgcgcc
      961 gacccgcgga tcagcgacgg ctggcgcgaa tcggtggccc ccacgacgag gtggctgaaa
     1021 cgatggccgg cgtcggaatc gaagtggctc tgcgtcccga cgaaatactc cagcaggttg
     1081 gcccgcgcgc cgatctcgaa gttgccgtcg gagtcgacga acgcctcgat ggagaggccg
     1141 gtggcgggga tgacttgcgc gtggtagatg taggtccagt catcctcggc ggcggcggcc
     1201 gacgagaggg cgtcgaccgc aagcgtaacc gtcgccccct gcggccggta gccgagcgaa
     1261 tagcgctgga tgacgcgggt gtcctcgccc tcaagctcgg accggttcag gttcgaccag
     1321 accgcgccca cggcgaaggg gctattgccg cgcgaggtga ggccgatgtt ccagtagtgg
     1381 cggtgatcgt agggactggg gccggacgga aagtaccggt aggaaccccc caccgagagc
     1441 gaggaaccga gcgacaggcc gccggcgagc gcgtattcct tgaaatcctc cccggcgtcg
     1501 ctgtcaatgc gccggtaagc cagtccgaat ccccgtcgcg tgatgacggt gccccagttt
     1561 ttcagaaccg aaccctcggc gaggtcgccc atgaaagcga cggtggcggg tttgtagggg
     1621 ccgaggacgg ccgggttgac ccaaaccgct tcggggccga acgcggtggc cgcgggcgca
     1681 tagtagaaca cgtcgtcggg aaaaggcact tcctgcgcct tcgccccgag cgggatggcc
     1741 gcggctgccg caagcagcgc ggtccagcga tgaaaccgca tcattcctct ccctggcctc
     1801 ggccacgaat gtagatgttg ccgctgccgc tgatggaact gctgatgaat ccttgtcccc
     1861 cgccgaccac caatccgagg cggtcgcgct ggacgaggtc ggtctggaac cgcagaccgg
     1921 acacttcgat ccggccgctc tcccccaccg cgagcgacat caccgccgac aggtcggcgg
     1981 cggcggtgat gtcgacatcc tcgaaccggt tgctcaccac caactggctc tcgcccaggt
     2041 cggcgatctc gagcgtgatc ggggcggact ggccgcggat cacattgcgc cgtccgagcg
     2101 gccggaagtc ggtgacgtcg atgaggccga aatcgttgcg gatgctgact tcgccggtga
     2161 gcccctcaat gcggatctcc ccgccgtcgt tgcggaacac cgcctggtgc tcccggctct
     2221 cgatatcgga ggccgtgagg gtcgagttgc tcgtccggac ggcaatgtcg ccggcgacgt
     2281 cctcgagaat cacgcggcgg ttggctgtgc cgaggtcgac cggtccccgc acccggtaga
     2341 cctccaggcg cccgagcgac gacgggttga ccaccccgct gaacggaccc tcggccgtga
     2401 cgtcaaaata gagcgcgtcg atatcgacga agacgttttc aggaacgcgg atttccgcgt
     2461 cgacgctccc cgactcggcg ccgctccacg gcgcggggtt gggcgcgcgc aactggagcc
     2521 gggcgccgtc ggcaacgcgg ccgatgtcga cagcgatgag gtcgatgtag tcgacggcgc
     2581 gggagcgcga gtcggtgacg gcctgcttgg tgtagacgac gacggcccgg tcggtttcgg
     2641 tagcgcggat ggtgatcttt ccggccagcg tggcggccga gcggacgagc agcttctcgc
     2701 cgggggccag cggggcggcg acagtcagcg ggcgggtggc gaagcggccc tcgccggcgt
     2761 cgtgaatttc ctgcgcccgg gccgggccga tacccaccac tgcggcggcc aacagcgcgc
     2821 aggcggcgcg ccgggccggt cggctatgag agcgagtcat aaatcccttt catgagtatt
     2881 gatcccgcga ccgccgcgtt gagcgactcg acctcggggg cgtgggccac gcggacggtg
     2941 caggccgcca gcgcccgcat ccggtcggac aggccctccg cctccgaccc caccgcgagc
     3001 gcgtagcggg ccgccgggtc cgggcggaga gattcgctca gcgtctgcgt ccccccgcgc
     3061 ccggccgcca gcagggtgat cccctcgctc gccaccagac gcgcgagggt gtcgcagtcg
     3121 gcggcggcga tccggacgcc gaagacggcc ccggccgacg aacgcacaac tttgggtgca
     3181 aacggctcag ccgtcgtccc gaccgtcacc accaaccgga aaccgaacgc gagcgcggac
     3241 cgcaggagca tgccgaggtt gcccggatcg gccacgtttt cgcacacaag aatggtacgg
     3301 tggcggcgcg cacaaagttc ggccgggtcg atccggggaa tggtaaagac gccgacgatg
     3361 ccctgcggag cggg
//
LOCUS       DAOOKS010000141         3356 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_34369_length_3356_cov_3.353226, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000141 DAOOKS010000000
VERSION     DAOOKS010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34369_length_3356_cov_3.353226"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="PK112_03180"
     CDS             <1..614
                     /locus_tag="PK112_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
                     /protein_id="HPC11085.1"
                     /translation="TIDSCTESGLTLTNSVSRVLRSRITDNAVHGVSVSGGTPYLASS
                     LISGNAQCGVYCANDAKPVIGSVAGAGCNLSRNGTFALYNDGAQTIYARENAWGDDDS
                     AGVAALIWDQVDDPSKGWVQFMPFVLGYVCGDANGDGVGNPGVADVTYLVAYLFRGGP
                     PPPEIDAADVNNDGAVRISDLAYLIAFLFRSGPAPYCWEYGPS"
     gene            759..2795
                     /locus_tag="PK112_03185"
     CDS             759..2795
                     /locus_tag="PK112_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017251867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HPC11086.1"
                     /translation="MRRTGLFLLFAILTPMWIAASGERKPTATGTITGTVTDKETGRP
                     VTSASVLVVGTAFGAQTDLDGRYRIDNVAPGSHLLRFSHVEYRTVELAGVEVAEGSTA
                     VRSVALEKNGAVKSDVRILGTKDKLGVPESSSQAPVTKEEIPMYRLPAEDQALRQPPD
                     AAAAGHGVEIFMRGGRAGEAEYITRENSLPPAHGGSAIVNGQPYDAMFFRDYGVNPFV
                     DTDDDHLSTFAVDVDDASYIMTRSYLQDGHLPPAEAVRTEEFINHFTYDYDPPRRETF
                     RVYADGAPSLFGEKTRLLRIGIKGREIDPEERKPATLVFVIDVSGSMARENRLGLVKQ
                     SLRYLVDQLLPDDRVGIVVYGSSGRVLLEPTGAGHRTRILRVIDGLEPGGSTNAEEGL
                     RLGYRMADRNFVRGQINRIILCSDGVANVGKTGPEEILRDIRRYAERGITLTAVGFGM
                     GNYNDVLMEQLGNKGDGFYAYVDDAAEARRVFIDNLTGNLQVIARDVKIQVEFDPRAV
                     RSYRLLGYENRDVADRDFRNDAVDGGEVGAGHQVTALYEIKLWSDGNRRDKHAIIDRR
                     EDDDGARPGDLGIVRIRYKDPDRDRVAEITGRITDEVFDRAFAEADPDFRLAAAAAEF
                     AEILRHSYWARDSRLSDVIRVAHRVRSETGSEEAAELADLIARAMHYEDELAER"
     gene            2986..>3356
                     /locus_tag="PK112_03190"
     CDS             2986..>3356
                     /locus_tag="PK112_03190"
                     /inference="COORDINATES: protein motif:HMM:NF028435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11087.1"
                     /translation="MAERFAARYRGAVDDYPAFLATLTRPPRNTFRVNTLKADVGATV
                     ARLRAIGLTITPLPWFEAAFAADPHINLAATLERFLGTIYIQEAASMLPPLVAAGELR
                     GARTVLDACAAPGSKTTLAAA"
BASE COUNT          548 a         1124 c         1148 g          536 t
ORIGIN      
        1 gcacgatcga cagctgcacc gagtccgggc tgacgctgac caacagcgtg tcgcgggtgc
       61 tccgctcccg gatcacggac aacgccgtgc acggcgtctc cgtcagcggc gggacgccct
      121 acctggccag cagtctgatc agcggcaacg cccagtgcgg tgtgtattgc gccaatgacg
      181 ccaagccggt catcggttcg gtcgccggag cgggctgcaa cctgtcgcgg aacggcacgt
      241 tcgcgctgta caacgacggc gcgcagacga tctacgcccg cgagaacgcc tggggggacg
      301 acgattccgc gggggtcgcg gcgctcatct gggaccaggt cgatgatccc tccaagggtt
      361 gggtgcagtt catgccgttc gtgctgggct atgtctgcgg agacgcgaac ggcgacgggg
      421 tcgggaaccc gggcgtggcc gacgtgacct acctagtcgc ctatctgttc cggggcggcc
      481 cgcctccgcc ggagatagac gcggccgacg tcaacaacga cggggcggtg cgaatctccg
      541 acctggccta cctgatcgcc tttctcttcc gctccggccc ggccccgtac tgctgggagt
      601 acggtccgag ctgagctttc ggtgtcggcc ggccccgacc gacctgtgca attttccacc
      661 ggcaccgctt ttcctgcgcc tttccgggaa gatgcgcggc tctccggtgt aatcccctcg
      721 aagacagcga tcaaccgccc tagttaagga ggtggcgtat gcgccgcaca ggactctttc
      781 tgctgtttgc gatcctcacc ccgatgtgga ttgccgcatc cggcgagcga aaaccaaccg
      841 ccacaggaac catcaccggt accgtcaccg acaaggagac gggccggccg gtgaccagtg
      901 cctcggtgct ggtcgtcggg accgccttcg gcgcccagac cgatctggac ggccgctacc
      961 ggatcgacaa cgtcgcgccg ggttcccacc tcctgcgttt cagccacgtc gagtaccgta
     1021 ctgtcgagct ggcgggcgtc gaggtggctg aaggatcgac cgccgtgcgg tcggtggccc
     1081 tggagaagaa cggcgcggtc aaaagcgacg tgcggatcct cggcacgaag gacaagctcg
     1141 gcgtcccgga gagcagcagc caggctccgg tgaccaagga ggaaatcccg atgtaccggt
     1201 tgccggcaga ggatcaggcg ctccggcaac ctccggatgc ggccgccgcc ggccacgggg
     1261 ttgagatctt catgcgcggc ggcagggcgg gcgaggcgga gtacattacg agggagaatt
     1321 cgctcccgcc ggcgcacggc ggctcggcga tcgtcaacgg gcagccctac gacgccatgt
     1381 ttttccgcga ctacggcgtc aacccgttcg tcgacaccga tgacgaccat ctctcgacct
     1441 ttgccgtcga tgtcgatgac gcctcctaca tcatgacccg ctcgtatctt caggacggcc
     1501 accttccgcc ggccgaggcg gtgcggacgg aggagttcat caaccacttc acctacgact
     1561 acgatcctcc gcggcgcgag actttccgcg tctatgccga cggggcgccc tcgctgttcg
     1621 gcgagaagac gcgcctcttg cggatcggca tcaagggacg agagatcgat cctgaggagc
     1681 ggaagccggc gaccttggtg ttcgtgatcg acgtctccgg gtcgatggcg cgggagaacc
     1741 ggctcggcct ggtgaagcag tcgctgcgct acctggtcga ccagctcctg ccggacgatc
     1801 gggtcggtat cgtcgtgtac ggctcctcgg gccgggtgct gctcgagccg acgggagcgg
     1861 gccaccggac caggatcctg cgcgtgatcg acggcctcga gccgggcggt tcgaccaatg
     1921 cggaagaggg cctccgcctg gggtatcgga tggccgaccg caacttcgtg cgcggacaga
     1981 tcaaccggat tattctgtgc tccgacggcg tggccaatgt cggcaagacc ggtccggagg
     2041 agattctccg cgacattcgg cgctacgccg aacggggaat cacgctgacg gcggtcgggt
     2101 tcggcatggg caactacaac gacgtgctca tggagcagct cggcaacaag ggggacgggt
     2161 tctacgccta tgtcgacgat gcggccgagg cgcgccgcgt gttcatcgac aacctgaccg
     2221 gcaacctgca ggtgatcgcg cgcgacgtga agattcaggt ggagttcgac ccgcgtgcgg
     2281 tccgcagcta ccgtctgctc gggtacgaga accgcgacgt ggccgaccgc gatttccgca
     2341 acgatgcggt ggacggcggc gaggtcggcg ccgggcacca ggtgacggcg ctgtatgaga
     2401 tcaagctgtg gagcgacggg aaccgccgcg acaaacacgc catcatcgac cggcgcgagg
     2461 acgatgacgg cgcccggccg ggcgatctcg gcatcgtgcg gatccgctac aaggatccgg
     2521 accgcgaccg cgtggcggag atcaccgggc gcatcaccga cgaggtgttc gaccgcgcgt
     2581 tcgctgaggc cgaccccgat ttccggctgg ccgccgcggc cgccgagttc gccgagatcc
     2641 tgcgccactc ctactgggcg cgggactcgc ggctgtccga tgtgattcgc gtggcgcacc
     2701 gcgtccggag cgagaccgga tcggaagaag cggccgaact ggccgacctc attgcgcgcg
     2761 ccatgcacta tgaagacgag ctcgccgagc gctgagcgcg ggagcagctc tttcgagggc
     2821 gcggccgaac gagggatcgg ccgcgctttt tcttggcgcg ccgcgcgggc gccggcggag
     2881 gatccggcgg aagcggcttg ccccgccgtt ccgttctgcc tatactgtcg cgctgtggag
     2941 aatctcgcgg gacatgacag aacgcggcgc gcggccgcag ccggcgtggc agagcgcttc
     3001 gccgcgcgct accggggggc ggtcgacgat tacccggctt tcctcgcgac cctgacccgt
     3061 ccgccccgca acaccttccg cgtaaatacg ctcaaggccg acgtcggggc gacggtcgct
     3121 cggctgcggg ccatcggatt gacaatcaca cctctgccgt ggttcgaggc ggcctttgcg
     3181 gccgacccgc acatcaacct cgccgccacg ctcgagcgct tcctggggac catctacatc
     3241 caggaggccg cctcgatgct tcccccgctg gtcgccgccg gggagctgcg cggcgcccgg
     3301 acggtgctcg acgcctgcgc cgcgccgggt tcaaaaacca cgctcgccgc ggcgat
//
LOCUS       DAOOKS010000142         3332 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_34683_length_3332_cov_3.963381, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000142 DAOOKS010000000
VERSION     DAOOKS010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34683_length_3332_cov_3.963381"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /gene="thyX"
                     /locus_tag="PK112_03195"
     CDS             <1..583
                     /gene="thyX"
                     /locus_tag="PK112_03195"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR02170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HPC11088.1"
                     /translation="RTANVNEYSGRYSVMKEEFYIPPEEEIRFQSAVNKQGRAEEEVP
                     PELKRRFVEFLRKIQADSYAEYAALVEAGLARELARVGLPLSLYTEWYWKIDLHNLFH
                     FLGLRLDRHAQQEIRAYAAVMAEMVQAVCPLAYEAFLDYQLQAVHFTGPELAILRPRL
                     TDFCPSEDELASGGLSKREAREFLEKLEKVRGE"
     gene            1220..2071
                     /locus_tag="PK112_03200"
     CDS             1220..2071
                     /locus_tag="PK112_03200"
                     /inference="COORDINATES: protein motif:HMM:NF020302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADAR domain-containing protein"
                     /protein_id="HPC11089.1"
                     /translation="MDRKREDTIVFTEDHRRYGAFCLGAPYVIRIDDVSWPTDRHYLL
                     GMKYPGVNPLRLRQTASYAEAVKLVQTEGAVERVKWDVVRDDYLERVLRAKFSQHLDL
                     EALLRSTGDHPLVYGNADDSYYGVGASGAGHNMFGRILMRVRGRVDRSVPEPHNAVIE
                     GCEEWVRRNPDDMSGFLRLTIVYLANRMPERASATAYRAIHLDPQDPRARIALAYALV
                     DQHRFGEALEHLRFVNRRGEADEDDLRLLATVYRALGRDVAADVLESRARRLEAEEED
                     DCDGDDF"
     gene            complement(2145..>3332)
                     /locus_tag="PK112_03205"
     CDS             complement(2145..>3332)
                     /locus_tag="PK112_03205"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: protein motif:HMM:NF014165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HPC11090.1"
                     /translation="ALAGQVWAEARKKSNFALFEPHLGGIVELVRRKADYYGYTAEKY
                     DALLEGYEPGARTAEVERAFAGLRTDLVELVEKIADAPRRPDIGVLKRPWDVDKQRTF
                     SRMLAAQIGYDFGAGRIDETVHPCCNDLGPLDVRILTRFYAEDLAEGLTGTIHEAGHA
                     FYTQSQRGSEHWGTPMGMTPSLAIHESQSRTWENIVGRSRHFWTYFFPQLRALFPEET
                     RDITLDDFYAAMNYVAPSYIRVEADEATYNLHIMLRFELERAIVEGDLAVKDIPGEWN
                     SRFRQYLGLAVDTDAHGCLQDVHWSDGSFGYFPTYALGNLYAAQFWSRAKADLPGLME
                     DFAAGQFDRLMTWLSENIHRQGFKVYPNELCRRLTGSDLSHRPLLDYLYAKYEEIYGI
                     RRN"
BASE COUNT          564 a         1022 c         1128 g          618 t
ORIGIN      
        1 ccgcaccgcc aatgtcaacg agtactcggg gcggtactcg gtcatgaaag aggagttcta
       61 cattccgccc gaagaggaga tccggtttca gtcggcggtg aacaagcagg ggcgggcgga
      121 ggaggaagtg ccgccggagc tgaaacggcg ctttgtcgag tttctccgga agatccaggc
      181 ggattcgtac gccgagtatg cggcgttggt cgaggcgggg ctggcccggg agctggcccg
      241 ggtcggattg ccgctgtcgc tgtataccga gtggtactgg aagatcgacc tgcacaacct
      301 gtttcatttc ctcgggctgc ggctcgaccg gcatgcccag caggaaatcc gggcgtatgc
      361 ggcggtgatg gcggagatgg tccaggcggt ctgcccgctg gcgtacgagg cgttcctgga
      421 ttaccagctc caggcggtgc atttcacggg gccggagctg gcgatcctgc gcccgcgtct
      481 gactgatttc tgcccgtccg aagatgagtt ggcatccggg ggattgtcga aacgggaggc
      541 gcgggagttc ctggagaaac tggagaaggt gcggggggag tgaccgcacc ttcgcacact
      601 ccgcggaccc tcggatcatg cgcctgcagt cgggccgccg ggagcgtccc gggccgagca
      661 ctttcggaac ctccttcagt ccgtcggcgg agaatcgacc ggcaggaccg ctcccggcga
      721 cccatcccgc accgcaactt ttctggaaat ttcgcttgac acgcgcggcc gcgcgtataa
      781 aataggaaac tccgccacca gagaagcgta ctatgctcac cacgtagcat tacttttctg
      841 tgcggggaaa gcctgccctc ggccgtgtgc cgggggaatg accgtaaact cttactcacc
      901 gggcccatgg tccgggacca gaggttgcca catgagtctc cacgcaactg atagaccagc
      961 ctggcgaatt ccgctaggta aggtggcctg acggtcgggc tgaggttttt ttccgctcga
     1021 atccgcttcg cgcctgcacg gggatgcccg tgaaggcctg cagttctcag ccccgccccg
     1081 acaggttacc gtcggcacaa tgacatggct cgctagtggc cgaggtcgac ccgtgtgttc
     1141 gggttgttct cgcccgcaga gggtcgcgtg tgcccaagtc acggtatgat gttcgtggac
     1201 catggaggtt gagaaccaga tggatcgcaa gcgcgaagac acgatcgtgt tcaccgagga
     1261 ccaccgccgc tacggcgcgt tttgccttgg tgccccctac gtcatccgca tcgatgatgt
     1321 atcgtggccg accgaccgcc actacctgct cggtatgaag taccccggcg tgaaccctct
     1381 ccgtctgcgg cagacggcgt catacgctga ggctgtgaaa ctggtgcaga cggagggcgc
     1441 tgtcgagcgg gtgaaatggg atgtggtgcg cgacgattac ctcgaacggg ttctccgggc
     1501 gaagttctcg cagcacctcg acctcgaggc gcttcttcgc agcacgggcg accatcccct
     1561 ggtctacgga aatgcggatg attcgtacta cggggttggc gccagcggag ccgggcacaa
     1621 catgttcggc cggatcctta tgcgcgtccg cgggcgcgtc gatcggagcg ttccggagcc
     1681 ccacaatgcg gtcatcgagg gctgtgagga atgggtgcgt cgaaacccgg acgatatgag
     1741 cggtttcctc aggctcacga tcgtttatct tgcgaaccgg atgcccgagc gcgcgtcggc
     1801 gaccgcctac cgggcgattc atcttgaccc gcaggatcct cgggcgcgga tcgctctcgc
     1861 ctacgccctg gtggaccagc accgcttcgg cgaagcgctt gaacacctgc ggttcgtcaa
     1921 tcgccgcggc gaggcggatg aggatgacct gcgcctcctc gcaaccgtct accgcgccct
     1981 ggggcgtgac gttgccgccg acgttctcga gtcgcgcgct cgccgcctgg aagccgaaga
     2041 ggaggacgac tgcgacggag acgacttctg agcggaggaa aggtcggggc cgccggcggc
     2101 cctgatctct cccggcgccg gcacttggat tgcgcccggg gcgctcagtt ccgccggatc
     2161 ccgtagatct cctcgtactt ggcgtacagg taatccagca gcgggcggtg ggacagatcc
     2221 gacccggtga ggcggcggca cagctcgttc gggtacacct tgaatccctg ccggtggatg
     2281 ttctcgctga gccaggtcat gagccggtcg aattgcccgg ccgcgaagtc ctccatgagg
     2341 ccgggcagat cggccttcgc ccgcgaccag aactgcgcgg cgtagagatt gccgagggcg
     2401 taggtcggga aatatccgaa cgagccgtcc gaccagtgga catcctgcag gcagccgtgg
     2461 gcgtccgtgt cgacagcgag gccgaggtac tggcggaagc ggctgttcca ctcgccgggg
     2521 atgtctttga ccgccagatc cccctcgacg atcgcgcgtt ccagctcgaa ccgcagcatg
     2581 atgtggaggt tgtaggtggc ttcgtcggcc tcgacgcgga tgtacgacgg ggcgacgtag
     2641 ttcatcgcgg cgtagaagtc gtccagcgtg atatcccggg tttcttcggg gaacagcgct
     2701 ctgagctggg ggaagaaata cgtccagaag tggcgcgagc ggccgacgat gttctcccag
     2761 gttcgggact gcgactcgtg gatagccagc gagggagtca tgcccatcgg cgtcccccag
     2821 tgttccgaac ctctctggct ctgcgtgtag aaagcgtgtc cggcctcgtg gatggtgccg
     2881 gtgagccctt cggcgaggtc ttcggcatag aaccgggtga ggatccgcac gtcgaggggg
     2941 ccgaggtcgt tgcagcaggg gtggacggtt tcgtcgatcc gcccggctcc gaaatcgtag
     3001 ccgatctgag cggcgagcat gcgggagaaa gtgcgctgct tgtccacatc ccaggggcgt
     3061 ttgagcacgc cgatgtccgg ccgccggggt gcgtcggcga ttttctccac cagttcgacg
     3121 aggtcggtgc gaagaccggc gaacgcccgt tcgacctcgg ccgtcctcgc ccccggctcg
     3181 tagccctcca gcagcgcgtc gtatttctcg gcggtgtacc cgtagtagtc cgccttgcgg
     3241 cggacgagtt cgacaatccc gcccaggtgc ggctcgaaca gcgcgaaatt cgactttttc
     3301 cgcgcctccg cccacacctg gccggccagc gc
//
LOCUS       DAOOKS010000143         3327 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_34748_length_3327_cov_3.005807, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000143 DAOOKS010000000
VERSION     DAOOKS010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3327
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34748_length_3327_cov_3.005807"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(246..2789)
                     /locus_tag="PK112_03210"
     CDS             complement(246..2789)
                     /locus_tag="PK112_03210"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HPC11091.1"
                     /translation="MSEKGRSAGGGRRLYGAIRSVAAGLAAALAAPLARASEADIVVP
                     DLNTGDFFGMTGHNLLLWGIVVCLLGMVFGWYAYRGLRKLPVHQAMRDISELIYETCK
                     TYLQTQGKFIMLLWAFIGLIIMVYFGWLRPVGAGNLLIILLFSLVGIAGSYGVAWFGI
                     RINTFANSRSAFASLTGKAYPTFAIPLRAGISVGTLLISIELIIMLAILLFVPGHLAG
                     YCFIGFAVGESLGAAALRIAGGIFTKIADIGSDLMKIVFNIKEDDARNPGVIADCTGD
                     NAGDSVGPTADGFETYGVTGVALITFILLAVMNPAIQVQLLVWIFVMRVMMIVTSIAS
                     YWINGALARGRYGDQATFNFEAPLTSLVWITSIVSIAMTYVISYAMLAELGGGSLWWK
                     LATIISCGTLAGAVIPELVKIFTSTKSRHVKEVVTAAREGGASLNILSGFVAGNFSGY
                     WMGLSIIALMAIAWGMSTSGLGAIMAAAPVFAFGLVAFGFLGMGPVTIAVDSYGPVTD
                     NAQSVYELSTIETIPNIAQDIKREFGFTPDFEKAKLTLEENDGAGNTFKATAKPVLIG
                     TAVVGATTLIFSIILLLATEAAGLEGVLTLEQINQALMMAGYHFSLLNAPFLLGLIAG
                     GAVIYWFTGASTQAVSTGAYRAVEFIKANIRLDGTSQKASVKDSKRVVEICTQYAQKG
                     MFNIFIVVFFTTLAFACVDSYFFIGYLISIAIFGLYQAIFMANAGGAWDNAKKIVETD
                     LREKGTPLHAATVIGDTVGDPFKDTSSVAMNPVIKFTTLFGVLAVELAIELTQGTRVA
                     LAALFFAIAAAFVYRSFYRMRITTTGVQEPAKPNRKAAAGEPAEVRAAR"
     gene            complement(3168..3317)
                     /locus_tag="PK112_03215"
     CDS             complement(3168..3317)
                     /locus_tag="PK112_03215"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPC11092.1"
                     /translation="MKKQVTARTPFGRIGRPEEVAEAVVFLCGDGASWITGQTLIVDG
                     GYSLM"
BASE COUNT          646 a         1073 c         1030 g          578 t
ORIGIN      
        1 ggatccaccc gcatgtcaaa gcgcttcatg agtaacactc cttcacggcg gccgctgtca
       61 agcggcagcc gggctgcaat ataccggcag gcggcgcgac tgtcaaggcg gcgcgggcag
      121 caccgcccgg gcccggaatt cgccggccgt gtgcggcgac accggctgtc acccgctcgc
      181 gccataaaga aaaccggccc gctcgaaaaa gcgggccggc tgtcaatact caatggaaac
      241 cgggctcagc gcgcggcgcg gacttccgcc ggttcgccgg ccgccgcctt ccggttcggc
      301 ttggccggtt cctgaactcc ggtcgtcgtg atgcgcatcc ggtagaacga gcggtagaca
      361 aaggcggccg cgatggcgaa gaacagcgcg gccagcgcca cccgcgtccc ctgcgtcagc
      421 tcgatcgcca gctccaccgc cagcaccccg aacagggtcg tgaacttgat caccgggttc
      481 atggccaccg aggaggtgtc cttgaacgga tccccgaccg tgtcgccgat caccgtcgcc
      541 gcgtggaggg gcgtgccctt ctcacgcagg tcggtttcga cgatcttctt tgcgttgtcc
      601 caggcgccgc cggcgttggc catgaagatc gcctggtaga gcccgaagat cgcgatcgag
      661 atgaggtacc cgatgaagaa gtaggagtcg acgcaggcga aagccagggt ggtgaagaag
      721 acgacgatga agatgttgaa catgcctttc tgcgcgtact gggtgcagat ttcgaccacc
      781 cgcttgctgt ctttcaccga ggccttctgc gaagtgccgt cgaggcggat gttcgccttg
      841 atgaattcga cggcgcggta cgcgccggtc gacacggcct gggtcgacgc cccggtgaac
      901 cagtaaatca ccgccccgcc cgcgatcagg cccagcagga agggcgcgtt caggagcgag
      961 aagtggtacc cggccatcat gagcgcctgg ttgatctgct ccagcgtcag caccccctcg
     1021 agccccgccg cctctgtcgc cagaagcagg atgatggaga agatgagggt ggtggccccc
     1081 accacggctg tgccgatgag caccggtttg gcggtcgcct taaacgtgtt cccggcgccg
     1141 tcgttctcct cgagcgtgag cttggctttc tcgaaatcgg gcgtgaagcc gaattccctc
     1201 ttgatgtcct gtgcgatatt cgggatcgtc tcgatcgtcg acagttcata cacggactgg
     1261 gcgttgtcgg tcaccgggcc gtaggagtcg accgcgatgg tgaccggccc catgccgagg
     1321 aagccgaacg cgaccaggcc gaaggcgaag accggcgcgg ccgccatgat ggcgcccagg
     1381 cccgaggtgc tcatccccca ggcgatcgcc atgagggcga tgatcgacag gcccatccag
     1441 tagccgctga aattgcccgc gacaaacccg gacaggatgt tcagcgaggc gcccccttcc
     1501 cgcgccgccg tcaccacttc cttcacgtgc cgcgacttgg tcgacgtaaa tatcttcacc
     1561 agctccggaa tcaccgcccc ggccagggtc ccgcacgaga tgatcgtcgc cagcttccac
     1621 cagagcgaac cgccgcccag ctccgccagc atggcgtagg agataacgta ggtcatggcg
     1681 atggagacga tcgaggtgat ccacacgagc gaagtcagag gggcctcgaa attgaaggtc
     1741 gcctggtcgc cgtaccggcc ccgcgccagc gcgccgttga tccagtagga ggcgatgctg
     1801 gtgacgatca tcatgacgcg catgacgaag atccacacaa gtagctgcac ctggatggcg
     1861 gggttcatga cggcgaggag gatgaaggtg atgagcgcaa cgcccgtcac gccgtaggtc
     1921 tcgaacccgt cggcggtcgg gccgaccgaa tcgccggcgt tgtcgcccgt gcagtcggca
     1981 atcacgccgg gattgcgggc gtcgtcttcc ttgatgttga agacgatctt catcagatcg
     2041 ctgccgatgt cggcgatctt ggtgaaaatg ccgccggcga tacgcagcgc cgcggccccg
     2101 agcgactcgc cgacggcgaa accgatgaag cagtaaccgg ccaggtgacc ggggacaaag
     2161 aggaggatcg ccagcatgat gatgagttcg atggagatga gcagggtccc cacgctgatg
     2221 cccgcccgca gcggaatcgc aaaggtcggg taggctttcc cggtcaggct ggcgaaggcc
     2281 gagcgggagt tggcaaaggt gttgatccgg atcccgaacc aggccacgcc gtaactcccc
     2341 gcgataccca caagcgagaa caggaggatg atcagcagat tgcccgcgcc caccggtcgg
     2401 agccagccga aatacaccat gatgatgagg ccgatgaagg cccagagcag catgatgaat
     2461 ttcccctggg tctggaggta cgtcttgcag gtctcgtaga tcagttccga aatgtcccgc
     2521 atcgcctggt gcacagggag cttgcgcagc ccgcggtagg cgtaccagcc gaacaccata
     2581 cccagcaggc agaccacgat cccccagagc aggaggttgt ggccggtcat gccgaagaaa
     2641 tcgccggtgt tgaggtcggg gactacaatg tccgcttcgc tggcccgcgc cagcggagcg
     2701 gccagcgccg ccgccaaacc cgccgccacc gaccggatcg ccccgtataa tcgccggccg
     2761 ccaccggcgc ttcgaccctt ctcactcata gattcgcgtc tccttttttc tactagtatc
     2821 gtcactcagg tcacatttgc ccgctgttga cacggccctg tattttcact tgaaggcacg
     2881 gcgtttgaaa agcggaaata tatgaatcgc gatcctcaac gcaacatttt tgatcggggc
     2941 ttgtgacgga tcgcgcaagc gacaccgggg gacagggatc cgtctgcggc actcccgatc
     3001 gctgaaattg gcctaacttc aatattgtca atgtcttttg cggagcacat aggtccgcaa
     3061 tataggtttt gatgtctggt caaacttacc gccttcttca cggtcacggc ccggccggtt
     3121 tcgcccgatc ggcctgtccg gaacgccccc ccggctccgc cgaccggcta catcaggctg
     3181 taccctccgt ccacaatcaa cgtctggccg gtaatccagc tcgccccgtc cccgcacaga
     3241 aacaccaccg cctccgcgac ttcctccggg cgcccgatgc gcccgaaagg agtgcgggcg
     3301 gttacctgct ttttcatctc ctcgtag
//
LOCUS       DAOOKS010000144         3319 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_34872_length_3319_cov_3.018076, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000144 DAOOKS010000000
VERSION     DAOOKS010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3319
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34872_length_3319_cov_3.018076"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..1140)
                     /locus_tag="PK112_03220"
     CDS             complement(70..1140)
                     /locus_tag="PK112_03220"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HPC11093.1"
                     /translation="MRRTLRRLTFAAVLIGGLGGCEDAPEGLYRQADSAVRRVRTQTT
                     GAYADSLLRQADSLLLSGRLEMARQNGRIGPFRDFAAAESLLTRALQTAVAASDSAKV
                     RHDRARDAALARRDELLTELKTWREALNGFLRLHGAEKRLTNAELGLQIGRRLIDSGK
                     YAEAMEVMDDVRDSLGRIRDIVDEYMRDESRHISTWRRWVNETIQESRATGTSALIVD
                     KAAHRAYLVSSGRLVKSWPADLGHNPSRQKLFAGDGATPEGMYRVTEVRRRGSKYYRA
                     LMLNYPNELDRRRFMENKKAGVISSGAKIGGLIEVHGEGGQNRDWTDGCVALSNEDID
                     ELFQHVQKGMPVTIVRRSDKWP"
     gene            complement(1245..1844)
                     /locus_tag="PK112_03225"
     CDS             complement(1245..1844)
                     /locus_tag="PK112_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11094.1"
                     /translation="MFKRVSLALVALAMLLAAAGCSKAPEAEMQASEAAMQAAVAAEA
                     EQYAPEAFQAALDTLNSAKALKAEQDGKFSLFRRYGKAAEMFVRAQTLSEQAATAAAE
                     EKERVRVLVAGLLTQADSVLTQATQLVDKAPRGKGNKAEIELMRGELSSATTALADAR
                     ADFDRGQFLVAKTKVEAVSQRLNSLINEVNTAFSRTGQR"
     gene            complement(2250..2474)
                     /locus_tag="PK112_03230"
     CDS             complement(2250..2474)
                     /locus_tag="PK112_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11095.1"
                     /translation="MRCAECGDKITGKPIKRGNRVFCSIDCAESAAQDRYDDEEDQEL
                     DDEFDTLDEEEVDFEEDSEDRFLGGEDDER"
     gene            3092..>3319
                     /locus_tag="PK112_03235"
     CDS             3092..>3319
                     /locus_tag="PK112_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HPC11096.1"
                     /translation="MNSHNPTPLPARLRDIAYAEDMTETAGNTPLVRLRVLPREWGIK
                     ATILVKPEFLNPTGSVKDRMAIYLLRQAERKG"
BASE COUNT          585 a         1088 c         1028 g          618 t
ORIGIN      
        1 ccggaggcga caggtagatg gcggcgaagg tgaccgtcag cgcgacgatg agcacaatca
       61 cgacatatct catggccact tgtcggagcg ccggacaatc gtcacgggca tccctttctg
      121 cacgtgctgg aacagttcgt cgatgtcctc gttggacagg gccacacagc catcggtcca
      181 atcgcggttc tgaccgccct cgccgtgaac ttcgatcagg ccgccgattt tggcgcccga
      241 ggaaatgacc ccggctttct tattctccat aaaccgccgg cggtccagtt cgttcgggta
      301 attcagcatg agggcgcggt agtacttgct gccgcggcgc cgcacttcgg tcacccggta
      361 catcccctcc ggcgtcgcac cgtcgcccgc gaacagcttc tggcgcgagg ggttgtggcc
      421 gaggtcggcc ggccaggatt tcacgagccg cccgctggac accaggtagg cccggtgcgc
      481 cgctttgtcg acaatgaggg ccgacgtccc ggtggcccgg gattcctgga tcgtttcgtt
      541 cacccagcgc cgccaggtgc tgatgtggcg ggactcatcg cgcatgtact cgtcgacaat
      601 gtcgcgaatc cgtccgagcg aatcccgcac gtcatccatc acctccatgg cctcggcgta
      661 cttgccggag tcgatcaggc ggcggccgat ctggaggccg agttcggcgt tggtcagccg
      721 tttttccgcg ccgtggaggc ggaggaaccc attgagcgcc tcccgccagg tcttaagctc
      781 ggtcagcagc tcgtcgcgcc gggcgagcgc ggcatcgcgg gcgcggtcgt gccggacctt
      841 ggccgaatcg gaggcggcca cggcggtttg gagcgcccgg gtgagcagcg actcggcggc
      901 ggcaaaatcc cggaacggcc cgatccggcc gttctgtcgg gccatttcca gacggcccga
      961 taggagcagg ctgtcggcct ggcgcagcag cgaatcggcg taggcgccgg tcgtctgcgt
     1021 gcgcacccgg cgcacagccg aatcggcctg ccggtacagc ccctcggggg catcctcgca
     1081 cccgcccagg ccgccgatca gaacggcggc gaaagtcagt ctgcgcagcg tccgtcgcac
     1141 ctgccgtcgt gccccgtcat cgaaggagtt ctcctacaag aacaccccgg agaccgattc
     1201 tgtgcaaggg tccccggggc cgtatgttgt cgcgggggca gcggtcagcg ctggcccgtg
     1261 cgcgagaagg cggtattgac ttcgttgatc aggctgttga ggcgctggct gacggcctcg
     1321 actttggtct tggcgaccag gaactgaccg cggtcgaagt cggcccgggc atcggcgagc
     1381 gcggtggtcg ccgaggacaa ctcgccccgc atgagctcga tctcagcctt gttccccttg
     1441 ccgcgggggg ccttgtcgac aagctgcgtg gcctgggtca acacgctgtc ggcctgcgtc
     1501 aacagaccgg ccaccagcac ccgcacgcgc tccttctctt cagcggcagc ggtagcggcc
     1561 tgctcagaga gggtctgggc gcggacgaac atctcggccg ctttcccgta gcggcggaac
     1621 agcgaaaact tgccgtcctg ctcggccttg agggccttgg cggaattcag cgtgtccagt
     1681 gcagcctgga acgcttcggg cgcgtactgc tcggcttcgg ccgcgactgc cgcctgcata
     1741 gcggcttcac tggcctgcat ttccgcttcc ggggctttcg agcaaccggc cgccgccaga
     1801 agcatcgcga gcgccactag agccagcgac acacgcttga acatatcaca ctacctcact
     1861 gccgggagtt gttggtttgc atgaaaaaaa agacatgctc aagtccccgg cgaacttgca
     1921 cacgtctggt tgcgttagca gtgatcctta acgtgccgcg catcccagat ccagccggca
     1981 ctccttggtt gtctctcttt tgcggttgtc gcgtcggtat ccggcgcgcg gtcgctcacc
     2041 ttcaaatctg tcctaactgg tccgagtttc cagcccggta atttatcgcg ccaatccggt
     2101 ttgtcaagga tataattttc gcccccgctc gccctcatga ccgatacccg ccggcgcgat
     2161 gatccggggc gcagcccatg caggaacagc gcccggaaaa atcggcccgg gagatgcccc
     2221 gcgcaacgac cgccggcgcc ctccgccggc taccgctcgt cgtcctctcc tcccaaaaaa
     2281 cggtcctcgg aatcctcctc aaagtcgacc tcttcctcat cgagcgtgtc gaattcatcg
     2341 tcgagttcct ggtcttcctc gtcatcgtac cggtcctggg cagcggactc ggcgcaatcg
     2401 atcgagcaga acacccggtt cccgcgcttg atcggcttgc ccgtgatttt gtcgccacac
     2461 tcggcgcaac gcattttcga ctccctcaga acaagttcaa ggtgctagcg gaaaccggct
     2521 aattatgtgc tccggtttcc gagaagcaaa ctttttttta caccgatgaa atgaggaaag
     2581 agttcgcaca cacaccggtc ccggatcaac aagcaccatc gtactatatt gattgacaat
     2641 gatataacaa aatatgaagg gtcccgcgtc cttggccggg aaacgggacc ctgaggaagt
     2701 ttattgacgg agccgcggtt ggctagcggc tcataatggg gtcggcatag aagaacagcg
     2761 cgagctgctc ggagagaaca cggtcggcga tcatggcgac acctctcagt tcggtcggtt
     2821 tcgtggacgg gcggtccgcc ggtgttcgtg attgaatata cgggcagccg ggcgggcggt
     2881 tgcggggaaa aggcgcgcgc ggccggatcg cgcacgatgc atcggctgtc tcgcgcgctc
     2941 ccgcgggtgc ggcccgccgc catggccttg acacaggttc tccgcgcccg ctatactacg
     3001 ggccggcgtc gaactttccc ggccctccgg tgttctggac cgtgcgaacg cccgaaaaca
     3061 acgacccaga caagagagag attatttgtc catgaacagc cacaacccca cacctctccc
     3121 ggcccgcctc cgggacattg cctacgccga ggatatgacc gagacagccg gcaacacccc
     3181 gctcgtccgg ctccgcgtgc tgccccggga atgggggatc aaggcgacga tcctggtcaa
     3241 accggagttt ctcaacccga ccggctcggt gaaagaccgc atggcgatct acctgctgcg
     3301 gcaggcggag cgcaaaggg
//
LOCUS       DAOOKS010000145         3317 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_34908_length_3317_cov_3.120478, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000145 DAOOKS010000000
VERSION     DAOOKS010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3317
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34908_length_3317_cov_3.120478"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="PK112_03240"
     CDS             complement(<1..590)
                     /locus_tag="PK112_03240"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HPC11097.1"
                     /translation="MTAELYEHEDIGKDEKDVSVRRAFRGLLPFLAAHKKGLALCLLL
                     LVAVTAMTLAWPWLIQQAIDKILRGQLDRPAAERDFSPLVKVGLTVVLLQIASLALMY
                     IQRVKLETIGQNVMLDLKRKLFDHILSLDVSYFDKNPVGRVMARVESDTESLRMLFTN
                     TVVLIVGDVVLIVGILGVMFYHHPRLALVLSLSLPAL"
     gene            complement(920..2641)
                     /locus_tag="PK112_03245"
     CDS             complement(920..2641)
                     /locus_tag="PK112_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11098.1"
                     /translation="MSIPRSTAVAAAAVVTLAAALMAGAPQVISFQGRLTDDLGIPVA
                     DGPADVRIIIWNDPTAALPENEVWNSGVVTVTTVGGLFSLNLGVSPMPALTPDLFADT
                     NLWVGLSIEGQAEGQPRIRLGSAAFAFRAVRADTAAYALASPGGTSDHGSLTGLLDDD
                     HPQYVRVDGSRALIGDLYMSGRLIRNLAPGQLASDAIRYDQAMKLGDPAGGDLAGTYP
                     DPQLAANSVGLAELAAGSVNSEKVLDNTLSALDLANGSVQTAEIADGTIVDADISPTA
                     AIAVDKVAGTAASLSGAQTVTGAKTFVGGVKIGSAGAPSASAALEVSKEFLSPGLVYG
                     LSLGAANNGAGSVHSLYAAASGPVAHSGYRTGVYALAGAADATAGYSRGLAAFGLGGE
                     TAYGVYAYASAGATNWAGYFDGDVAVTGTLSKAAGSFRIDHPLDPENRYLQHSFVESP
                     DMKNIYDGVVTTDAAGYAVVTLPDYFEALNRDFRYQLTVIGDFAQAIIAEKVAGGRFV
                     IRTDKPNIEVSWQVTGIRHDPYAETHRIAVELDKPARERGRYQNPEAYGLGSDRSINR
                     AERTVQE"
     gene            complement(2801..>3317)
                     /locus_tag="PK112_03250"
     CDS             complement(2801..>3317)
                     /locus_tag="PK112_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11099.1"
                     /translation="RRLRVPPGGHLPAMSVLVRCLAGLGAVVLAAAAALIGCAPPPPV
                     ADQPTVPRGFYDEVWAEPRIVHADSVFTLIRADRKDSVFVHTNEVDPNAWAPALEFKV
                     APDTCFVSVTLHFLESDRPALPLLARYLPKGYYKLTVNNPLRFGETVYRGSCLLGATV
                     CGRRQTVPIVR"
BASE COUNT          573 a         1025 c         1154 g          565 t
ORIGIN      
        1 agcgcgggca gcgacagcga cagcacgagc gccagccggg ggtggtggta gaacatcacg
       61 ccgaggatcc cgacaatcag gacgacgtcg cccacgatga gcacgaccgt gttggtgaag
      121 agcatgcgca gcgactcggt gtccgattcc acccgcgcca tcacgcgccc gaccgggttc
      181 ttgtcgaagt aggagacgtc gagcgagagg atgtggtcga acagcttgcg cttcaggtcg
      241 agcatgacat tctgcccgat cgtctcgagc ttgacgcgct ggatgtacat gagcgccagg
      301 gaggcgatct ggagcagcac cacggtcagc ccgactttca cgagcgggga gaaatcccgc
      361 tcggcggcgg ggcgatccag ttgtccgcgc aggatcttgt cgatcgcctg ctggatcagc
      421 cagggccagg cgagggtcat ggcggtcacg gcgaccaaca ggagcaggca gagcgccaga
      481 ccttttttat gagcggccag gaacggcagc aggccgcgaa aagcccggcg gacgctgacg
      541 tccttttcgt ctttgccgat gtcctcgtgt tcgtacaatt ccgcagtcat tgtcggcctc
      601 gttcacagtc tcgattggtg cggcggagcc gcggccgatc cggcgaaaaa aaacccgccg
      661 gaccctgtct cggtgcggcg ggggaaaatt ctctcgtggg agcggcctaa acagacacac
      721 tcctcccgcc gcatttcttc ccgttcggca tggcagccga gatgtagacc ttaagctgga
      781 tcacggaatc ctcctgagtc tatcactatg cagcggtcaa caattcatca cagcattagg
      841 acggcgtctc cgtccccttt gtttcgccaa gtaacgcctt tttttgtttt tgtcaatcgc
      901 ttttttttca gtctcgcggc tactcctgca ccgtccgctc ggcgcggttg atcgaccggt
      961 ccgaccctaa tccgtaggcc tcggggttct ggtagcggcc gcgctcccgc gccggtttgt
     1021 caagctcgac cgcgatgcgg tgggtctccg catagggatc gtgccggatc ccggtcacct
     1081 gccatgagac ctcgatgttg ggcttgtcgg tcctgatgac gaaccgtccg ccggcgactt
     1141 tttcggcgat gatcgcctgg gcgaagtccc cgatcacggt gagctggtag cggaaatccc
     1201 ggttgagggc ctcgaagtag tcgggcagcg tcacaacggc gtagcccgcg gcatcggtcg
     1261 tcaccacccc gtcgtagatg ttcttcatat cgggggactc gacgaaggaa tgctggaggt
     1321 acctgttttc cggatcgagc gggtggtcga tgcggaacga cccggcagct ttggagaggg
     1381 tgccggtgac ggcgacgtcg ccgtcgaagt accccgccca gttggtggcg ccggcggagg
     1441 cgtaggcata gacgccgtag gcggtttcgc cgccgaggcc gaaagcggcg aggccgcgcg
     1501 aatagccggc ggtggcgtcg gccgccccgg ccagggcata gaccccggtc cggtagcccg
     1561 aatgcgcgac cggcccggag gcggcggcat acaggctgtg gaccgaaccc gcgccgttgt
     1621 tggcggcccc gagactcaga ccgtagacga ggccgggact cagaaattcc ttgctcactt
     1681 cgagagccgc ggaggcggag ggggcgccgg cgctgccgat tttgacgcct ccgacaaacg
     1741 tcttggcgcc cgtgactgtc tgggcgccgg agaggctcgc ggccgtcccg gccactttgt
     1801 cgaccgcgat cgcagcggtc ggcgagatgt cggcgtcgac aatggtgccg tcggcgatct
     1861 cggcggtctg caccgagccg ttggccaggt cgagcgcgct gagggtgttg tcgagcactt
     1921 tctcggagtt caccgagccg gcggccagtt ccgccaggcc caccgagttg gccgccagtt
     1981 gcggatcggg ataggtgccg gccaggtcgc ccccggcggg atcgcccagc ttcatcgcct
     2041 ggtcatagcg gatggcatcg gaggcgagct gtcctggggc caggttccgg atcaggcgcc
     2101 ccgacatata caaatcgccg atcagcgcgc ggctgccgtc gacccgtacg tactgggggt
     2161 ggtcatcgtc gagcagtccg gtgaggctgc cgtggtcgga ggtcccgccc ggcgaggcca
     2221 gggcgtacgc ggcggtgtcg gcgcgcacgg cgcggaaagc gaaagccgcc gagccgagtc
     2281 tgatccgcgg ttgtccctcc gcctgaccct cgatcgagag cccgacccag aggttggtat
     2341 cggcgaagag gtccggagtg agcgcgggca tcggggaaac gccgaggttg agggagaaga
     2401 ggccgccgac agtagtgacg gtcaccaccc ccgaattcca gacttcgttt tcgggcaggg
     2461 cggcggtcgg gtcgttccag ataatgatgc gcacgtccgc cggaccgtcc gctaccggga
     2521 tgccgaggtc atcggtgagc cggccctgga acgaaatcac ctggggggcg ccggccatga
     2581 gggcggcggc gagagtcacg accgcggccg ccgccacggc ggtggatcgg ggaattgaca
     2641 tagcttatct ccttgcgatt tgctgctctg gcaaaaccgt cgggcggcgt tgtgcgatgc
     2701 cggccaggaa cgatgcggga acctgagaaa ttccgccgcc ttgtgcaaga tactaggaag
     2761 ttcaattctg tcaaggcaac acggagcgcg cgatgccctc tcaccgcacg atcggcaccg
     2821 tctgacggcg gccgcacacg gtcgcaccga gcaggcatga gccccggtac accgtctccc
     2881 cgaacctgag cggattgttg accgtgagct tgtagtaacc cttcggcaga tagcgcgcca
     2941 gaaggggaag cgcggggcgg tcggactcga ggaagtgcag ggtgaccgac acgaagcagg
     3001 tgtccggggc gaccttgaac tcgagggccg gcgcccaggc attggggtcg acttcgttgg
     3061 tgtgcacgaa caccgagtct ttccggtctg ctcgaatcag ggtgaaaacg gagtcggcgt
     3121 ggacaatgcg cggctccgcc cagacctcgt cgtagaaacc gcgcgggacg gtgggctggt
     3181 cggccactgg cgggggcggg gcgcacccga tcagcgccgc ggccgcggcc agcacgaccg
     3241 cccccaggcc ggcgagacag cggacgagca cgctcatggc aggtaggtga cctcccggcg
     3301 gtactcgtag tcgccga
//
LOCUS       DAOOKS010000146         3312 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_34978_length_3312_cov_3.699724, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000146 DAOOKS010000000
VERSION     DAOOKS010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34978_length_3312_cov_3.699724"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            14..973
                     /gene="pstC"
                     /locus_tag="PK112_03255"
     CDS             14..973
                     /gene="pstC"
                     /locus_tag="PK112_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HPC11100.1"
                     /translation="MQPYQFRPKSKIREFLGERLIQVTALTALVSVALIFIFIFKEAV
                     PIFTDESVREEVTLEKMVVKQEWKAGREASCKWQPESGTPKYSLLPLFVGTLKAALIA
                     MLFAVPLGVGAAIYSAEFASRRLREFIKPVVELLAGIPSVVLGFFALLVLATVVQNAL
                     GLTYRLNAITAGLALGLAVIPIVFTVAEDAMTSVPQSLRDAALALGANRWQVSRTVVL
                     PAALPGIAAGVILGFGRAIGETMIVLMASGNAAVVSLAPGDSVRTLSATIAAELAEVV
                     FGSAHYSVLFLLGTLLFVFTFLINLGGDVALRRIRTRLQGRTS"
     gene            970..1935
                     /gene="pstA"
                     /locus_tag="PK112_03260"
     CDS             970..1935
                     /gene="pstA"
                     /locus_tag="PK112_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HPC11101.1"
                     /translation="MSAVPRGLAARPGPPAPDFQLKAKGTGLLPKSLTLAAVAIIALM
                     LAVILANILIGGREVLSVDFLTHPPEQGMQAGGIFPAIFGTVALVLLMTIAVVPIGVL
                     TAVYLHEYTRPDSKITRLIRIAISNLAGVPSIVFGLFGLGFFIGFVGGGLDAVFHGGA
                     PGPVWGQPAIVWAAATLALLTLPVVIISTEEALRTIPGELKDASYALGATKLQTIRRI
                     ILPQAMPGILTGGILAMSRGAGEVAPIMFTGAAYYLPYLPTRLNDQFMELGYHIYVMA
                     TQSPDIEATKPILYGTVLVLLVLTFVLNFTAIIIRSRLRARRPNA"
     gene            1928..2833
                     /gene="pstB"
                     /locus_tag="PK112_03265"
     CDS             1928..2833
                     /gene="pstB"
                     /locus_tag="PK112_03265"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015774826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HPC11102.1"
                     /translation="MPEDTPPTADNRPRPTPPALPDGEPARPGMADPLRAPQVRFARP
                     SEPEGETACKITVDHQNFFYGHHQALFDINLQIAEKRVTALIGPSGCGKSTFLRTLNR
                     MNDTIPGTRMEGTVRLGGLDIYRDIADLSTLRTRVGMVFQKSNPFPKSVFDNVAYGLR
                     VNGVTDRQIVAASVEDALRRSFLWDEVKDKLDQSAYMLSGGQQQRLCIARALAVRPEV
                     LLMDEPASALDPISTTKIEDLIGDLRDNYTIVIVTHNMQQAARISDYTAFFYEGRLIE
                     YGPTRRIFTKPSLKRTEDYVTGRFG"
     gene            2883..>3312
                     /locus_tag="PK112_03270"
     CDS             2883..>3312
                     /locus_tag="PK112_03270"
                     /inference="COORDINATES: protein motif:HMM:NF014010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HPC11103.1"
                     /translation="MTRHLQREIERIKKKILSLSAMVEERLHLSVKSIAERNSEMALR
                     VCESDWEIDRMEVEVEEDCLKILALHQPVAIDLRFLIAVLKINSDLERIGDLAAGIAE
                     QGIFLAARPRVVLPFDFPRMAEIAKDMLKGAIDSLVSMNPD"
BASE COUNT          543 a         1120 c         1060 g          589 t
ORIGIN      
        1 cgggtgagta agtatgcagc cctaccagtt ccgaccgaaa tcgaagatca gggagttcct
       61 gggcgagcgc ctcatccagg tcacggcgct caccgccctg gtctcggtcg cgctcatctt
      121 catctttatc ttcaaggaag ccgtcccgat tttcaccgat gagtccgtcc gcgaggaagt
      181 cacgctggag aaaatggtcg tcaagcagga gtggaaggcg gggcgggagg cgtcgtgcaa
      241 atggcagccg gagtccggga cgccgaagta ctcgctgctg ccgctgtttg tggggacgct
      301 gaaggcggcg ctcatcgcca tgctattcgc cgtcccgctc ggcgtggggg cggcgatcta
      361 ctcggccgag ttcgcctccc gccgcctgcg ggagttcatc aagccggtgg tggaactgct
      421 ggcgggcatt ccctccgttg tcctcgggtt cttcgccctg cttgttttgg cgacggtggt
      481 gcagaacgcg ctcgggttga cttaccggct gaacgcgatc accgccggcc tggccctcgg
      541 cctcgccgtc atcccgattg tcttcacggt ggcggaggat gcgatgactt cggtgccgca
      601 gtcgctgcgc gacgcggcgc tggcgctcgg ggccaaccgg tggcaggtct cgcggacagt
      661 cgtgcttccg gccgccctgc cgggcatcgc cgccggcgtc atcctcgggt tcgggcgcgc
      721 gatcggggaa acgatgattg tgctgatggc ctcggggaac gccgcggtcg tctcgctcgc
      781 gcccggcgat tcggtgcgga cgctctccgc gaccatcgcc gcggaactgg ccgaagtcgt
      841 gttcgggagc gcccactaca gcgtgctctt tctgctcgga accctcctgt tcgttttcac
      901 cttcctcatc aacctcgggg gcgacgtggc gctgcgccgg atcaggacgc gcctgcaggg
      961 gaggacgtca tgagcgccgt cccccgcggc ctcgcggcgc ggcccggccc gccggcgccc
     1021 gactttcagt tgaaagccaa aggcacgggg ctgcttccga agtccttgac cctggcggcc
     1081 gtcgcgatta tcgcgctcat gctggccgtc attctcgcca acattctgat cggcggccgg
     1141 gaagtcctct cggtcgattt ccttacccat ccgccggagc aggggatgca ggcggggggt
     1201 attttcccgg cgatcttcgg gaccgtggcg ctggtgctgc tcatgacgat cgcggtggtg
     1261 ccgatcgggg tgttgacggc cgtctacctg cacgaataca cgcggcccga ctcgaagatc
     1321 acgcggctga tccgcatcgc gatcagcaat ctcgccgggg tgccatcgat cgtcttcggc
     1381 ctgttcggac tggggttttt catcgggttc gtcggcggcg ggctcgacgc ggtgtttcat
     1441 ggcggggcgc cgggcccggt gtggggacag ccggcgatcg tctgggcggc ggccacgctc
     1501 gccctgctga cccttcccgt ggtgatcatc tcgaccgagg aggcgctgcg gaccatcccg
     1561 ggagagctca aggacgccag ctacgcgctc ggggcgacca aactgcagac gatccggcgg
     1621 atcattctcc cgcaggcgat gccggggatt ctcaccggcg gcatcctggc catgagccgc
     1681 ggcgccgggg aggtcgcgcc gatcatgttc accggggccg cctactacct gccctacctc
     1741 cccacccggc tcaacgacca gttcatggag ctcggctacc acatctacgt catggcgacc
     1801 cagtcgcccg acatcgaggc caccaagccg atcctctacg gcactgtttt ggtgttgctg
     1861 gtgctgacct ttgtccttaa cttcaccgcg atcatcattc gatcgcgact gcgcgcgagg
     1921 cgtccgaatg cctgaagaca caccgcccac cgccgacaac cggccgcggc cgacgccgcc
     1981 tgctttgccc gacggagaac cggcccgccc ggggatggcc gatccgctcc gcgcgccgca
     2041 ggtgcggttc gcgcgtccgt ccgagcccga gggcgagacc gcctgcaaga tcaccgtgga
     2101 tcaccagaac ttcttctacg gccaccacca ggcgctcttc gacatcaacc tgcagattgc
     2161 ggagaagcgc gtcaccgcgc tcatcggacc ctccggctgc ggcaagtcga ctttcctgcg
     2221 caccctcaac cggatgaacg atacgatccc gggcacgcgc atggaaggca cggtgcggct
     2281 gggcggtctc gacatctacc gcgacatcgc cgacctctcc accctgcgga cgcgggtggg
     2341 gatggtgttt cagaagtcca acccgtttcc caagtcggtg ttcgacaacg tcgcctacgg
     2401 cctgcgggtc aacggcgtga ccgacaggca gattgtcgcc gcgtcggtcg aggatgcgct
     2461 ccggcggtcg tttctgtggg acgaggtgaa ggacaaactc gatcagagcg cctacatgct
     2521 ctcgggcggg cagcagcagc ggctgtgcat cgcgcgggcg ctcgcggtcc ggcccgaggt
     2581 gctgttgatg gacgagccgg cctccgccct cgaccccatt tccaccacca agatcgagga
     2641 cctgatcggc gatctgcgcg acaactacac gattgtcatc gtcacccaca acatgcagca
     2701 ggcggcccgc atctccgact acaccgcctt cttctacgag gggcgcctga tcgagtacgg
     2761 gccgacgcgg agaatattca cgaagccgtc gctcaagcgg accgaggact acgtcaccgg
     2821 ccgattcggc tgaggcggcc cgacgcaaac cacacacgcc cggaaaaagg gaacccgatc
     2881 gcatgacccg acacctgcag agagaaatcg agcggatcaa gaagaagatc ctgtcgctca
     2941 gcgcgatggt cgaagaacgc ctccacctga gcgtgaaatc gatcgccgaa cgcaactccg
     3001 agatggcgct gcgcgtgtgc gagtcggact gggagatcga ccgcatggag gtggaggtcg
     3061 aggaggactg cctgaaaatt ctcgccctcc accagccggt cgcgatcgat ctgcgcttcc
     3121 ttatcgccgt cctcaaaatc aacagcgacc tcgagcgcat aggagatctc gccgccggca
     3181 tcgccgagca ggggatcttc ctcgccgccc ggcccagggt cgtcctcccc ttcgacttcc
     3241 cccgcatggc ggagatcgcc aaggacatgc tcaaaggggc gatcgactcc ctcgtctcta
     3301 tgaaccccga cc
//
LOCUS       DAOOKS010000147         3288 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_35334_length_3288_cov_3.305908, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000147 DAOOKS010000000
VERSION     DAOOKS010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35334_length_3288_cov_3.305908"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..904
                     /locus_tag="PK112_03275"
     CDS             <1..904
                     /locus_tag="PK112_03275"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HPC11104.1"
                     /translation="RLAPSRTGDLYKAWDTVLERFVALKLFDLDLMDHIPYRAQVLST
                     LRALADTDHPNICSLYGVHKFHGRYVVALELVEGESLRERLRRGPLDIDAFLRVASAL
                     AGALNHAHSRHIVHGSLTPSNILLRDDGDVRVVDFGFSTMIVEREGRGFSPSAEAVRY
                     RAPEQVTGEEVGPLADLFAAGAILYECLAGCPAFPGSSVEAVEEAVLHADPDFRLLQP
                     DRKTPADIVLLLERLLAKNPADRLRHAAELQITLTEIVSFEKAAPAREFFQVAPQSPR
                     QYLMLSLLAALLIIFWLVITSVHS"
     gene            1059..>3288
                     /gene="lon"
                     /locus_tag="PK112_03280"
     CDS             1059..>3288
                     /gene="lon"
                     /locus_tag="PK112_03280"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HPC11105.1"
                     /translation="MSTFKIVHNGELVTVNSRLCVIPLRDVVVFPHMIYPLLIGREFT
                     VKALREAMEGDRQVLLLAQKVAGVDNPGQEDLYDVGLVARILQVMKMPNGTFKVLVEG
                     LVRACVTSYVRTEDYVQVRISVQPPEEPAHDRELEALSRSVMELFTEYVHLNRRVPDE
                     VLVALASIEQYHQQADTIASHLLVRKEAKQEILELFDVRKQFLELARLLKEEIEILKI
                     EHKIDGDVRESMSRSQREFYLQQQLKAIKDELGQFDEPGAEVDDLYSQLESRDYPETV
                     VEKADEEIKKLAKMHPYSAEAGVIRGYVEWLLALPWNDVTQDRVDFKEVRAILDGDHY
                     GLEKAKQRILEHLAVIRLAGKVRGPILCLVGPPGVGKTSVGRSIARALDRKFVRMSLG
                     GVHDESEIRGHRRTYIGAMPGRIIQGLKRAGSANPVFLLDEIDKIGTDFRGDPAAALL
                     EVLDPEQNNSFSDNYLELECDLSNILFITTANTVATVPPALRDRMEIIRLPGYLDFEK
                     AAIARQFLLPKLVEQIGLQGVAVDFQDEALSLLIRQYTRESGVRELERQMGALLRKAA
                     VEVAEGRKLRRLTVTKRKIYALLGAPKYVDNNIKAEPTIGYAVGLAWTEMGGEVLPVE
                     VAPMAGKAKLTMTGSLGDVMQESATAALSYIRNHAAMFKLKENFFEDIDLHVHLPEGA
                     VPKDGPSAGVTLLIAMLSSLTETPVRTDVAMTGEVTLTGDVLAVGGLNEKLLAAKRMG
                     VLQ"
BASE COUNT          577 a         1144 c          996 g          571 t
ORIGIN      
        1 gcgcctcgcc ccgagccgga ccggcgatct ctacaaggcc tgggacaccg tcctcgagcg
       61 attcgtcgcc ctgaagcttt tcgacctcga tctcatggac cacatcccgt accgggcgca
      121 ggtgctgtcg actctgcgcg cgctggccga caccgaccac ccgaacatct gctcgctcta
      181 cggcgtccac aagttccacg gccgctatgt tgtcgccctg gagctcgtcg agggggaatc
      241 gctgcgcgag cgtttgcgcc gcggtccgct cgatattgac gcattcctcc gcgtggcttc
      301 ggcgctcgcg ggcgccctca accacgccca ctcgcgccac attgttcacg gcagcctcac
      361 gcccagcaac atcctgctgc gggacgacgg cgacgtccgg gtggtggatt tcggtttctc
      421 cacgatgatc gtggagcggg agggccgggg cttctcgcct tccgccgagg ctgtccgcta
      481 ccgcgcgccc gagcaggtca ccggcgaaga agtgggtccg ctcgccgacc tgttcgcggc
      541 cggggccatc ctctacgaat gcctggccgg ttgccccgct ttccccggat cgtcggttga
      601 ggcggtcgag gaagccgtgc tccacgccga ccctgatttt cgcctactgc aacccgaccg
      661 caagaccccg gccgacatcg tcctcctgct cgagcgtctg ttggcgaaaa accccgccga
      721 ccgcctgcgc catgccgccg aacttcagat caccctgacc gaaattgtca gttttgagaa
      781 ggcggctccc gcccgcgagt ttttccaggt cgccccgcaa tctccccgcc agtacctcat
      841 gttgtcgctg ttggctgctc tgctgatcat tttctggctg gtgatcacca gcgtgcattc
      901 gtgacctccg gttaagccgc accgtcccaa tgtgttcgcc gggcgcagac gccccgccga
      961 gccccgaccg ccgaaaaagg tgtttgccgc cgcaccgctt cgggctatct ttgacctgtc
     1021 ccggtaacca aggacccgtg gtaaggaggc cgattccggt gtcgacattc aaaattgtcc
     1081 acaacggcga actcgtgacc gtcaacagcc gcctgtgcgt gatccccctg cgcgacgtcg
     1141 tcgtgttccc ccacatgatc tatccgctcc tgatcggccg ggaattcacg gtcaaggcgc
     1201 tccgcgaggc catggagggg gaccgccagg tcctgcttct ggcgcagaag gtggcgggtg
     1261 tcgacaaccc gggacaggag gacctctacg atgtcggcct cgtcgcccgc atcctccaag
     1321 tcatgaaaat gcccaacggg acgttcaagg ttctcgtcga gggactcgtc cgcgcctgcg
     1381 tcacctccta tgttcggacc gaagactacg tgcaggtgcg catctctgtc cagcccccgg
     1441 aggagccggc gcacgaccgc gagctcgagg ctctctcccg ctccgtcatg gagctgttca
     1501 ccgagtatgt ccatctcaac cggcgcgtgc ccgacgaggt actcgtggcg ctggcctcca
     1561 tcgagcagta ccaccagcag gccgacacga tcgcatccca cctgttggtc cgcaaggagg
     1621 ccaagcagga gatcctcgag ctgttcgatg tccgcaagca gtttctcgaa ctcgcccgcc
     1681 tgctgaagga ggagatcgag atcctcaaga tcgagcacaa gattgacggc gacgtgcgcg
     1741 agtcgatgtc ccggtcgcag cgcgagttct acctccagca gcagctcaag gcgatcaaag
     1801 acgaactggg gcagttcgac gagccggggg ccgaggtcga cgacctctac agccagttgg
     1861 agagccgcga ttaccccgag accgtcgtgg aaaaggccga cgaggagatc aagaagctcg
     1921 ccaagatgca cccgtactcg gccgaggccg gcgtcatccg cggctacgtg gagtggctgc
     1981 tggcgctccc ctggaacgac gtcacccagg accgggtcga tttcaaggaa gtccgcgcca
     2041 ttctcgacgg cgaccactac ggcctggaga aggcgaagca gcggatcctc gaacacctcg
     2101 cggtcattcg cctggcgggc aaggtgcgcg gtccgattct ctgcctcgtc ggcccgccgg
     2161 gggtcggcaa aacctctgtc ggccgctcca tcgcccgcgc cctcgaccgc aagtttgtcc
     2221 gcatgtcgct cggcggggtg catgacgaat cggagattcg cggccaccgc cgcacgtaca
     2281 tcggcgccat gcccggccgc atcatccagg gactgaagcg tgccggctcg gccaacccgg
     2341 tcttcctttt ggacgaaatc gacaaaatcg gcaccgactt ccgcggcgat ccggcggccg
     2401 cgcttctgga ggttctcgac cccgagcaga acaattcgtt ctccgacaac tacctcgaac
     2461 tggagtgcga cctctcgaac attctcttca tcaccaccgc caacaccgtc gccaccgtcc
     2521 cccccgcgct ccgcgaccgc atggagatca tccgcctccc cggctacctg gacttcgaga
     2581 aggccgcgat cgcccgccag ttcctcctgc cgaagctggt cgagcagatc ggtctgcagg
     2641 gggtcgcggt cgactttcag gacgaggccc tgtcgctgct catccgccag tatacgcgcg
     2701 agtccggcgt ccgcgaactc gagcgccaaa tgggggcgct cctgcgaaag gcggccgtgg
     2761 aagtcgccga ggggcggaag ctgcgccgcc tgacggtcac caagaggaaa atctacgctc
     2821 tcctcggcgc ccccaagtat gtcgacaaca acatcaaagc ggaaccgacg atcggctatg
     2881 cggtcggcct ggcctggacg gaaatggggg gcgaggtcct cccggtcgag gtggcgccca
     2941 tggccggcaa ggcgaaactg acgatgacgg gatcgctcgg cgacgtcatg caggagtcgg
     3001 cgacggccgc gctctcgtat atccgcaacc acgccgcgat gttcaaactc aaggagaatt
     3061 tcttcgagga tatcgacctc cacgtgcacc tgcccgaagg ggcggtgccg aaagacggtc
     3121 cgtccgccgg cgtcactctc ctcatcgcca tgctctcgtc gctcaccgag accccggtcc
     3181 gcaccgacgt cgccatgacc ggcgaggtga cgctgaccgg cgatgtgctc gcggtggggg
     3241 gcctgaacga gaaactcctc gccgccaaac gcatgggagt gctgcagg
//
LOCUS       DAOOKS010000148         3288 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_35337_length_3288_cov_2.733375, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000148 DAOOKS010000000
VERSION     DAOOKS010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35337_length_3288_cov_2.733375"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="PK112_03285"
     CDS             <1..233
                     /locus_tag="PK112_03285"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPC11106.1"
                     /translation="TVSDNGTGMSDETRSKIFTLHFTTKKGGHGLGLSNCKKIIQAHH
                     GDLLVDSTPGEGTEFRVILPRFQPEKPKSDKK"
     gene            230..3214
                     /locus_tag="PK112_03290"
     CDS             230..3214
                     /locus_tag="PK112_03290"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPC11107.1"
                     /translation="MTSPALPPGYCRTAPLGRGGTAEVVRAFSAALGREVALKIPLAD
                     ADPSGAAPFAALVRRERLLIGGRRFPGLVRLIEAGEAPPYVAMEICPGPTLDRLGKID
                     SLSLALNLLAALTADLEYLRAAGIVHGDLKPQNVFLPAAWEGLDGRQLFWVKLSDFSL
                     GRADGESDSDRAGLGTVGYLAPETIVGGAAEHQSDLFALGVTAYQMLTGLHPFMNGET
                     EPAAINSRCREEEPPQVDTLRPDVPAALSRLIARLLHKDRAQRPVSAWEVCLELERVG
                     AEYPFRRALQPGHFLRAGASWEDARDGLPVEWGDYTDYLAALSWGSPAQLRMILTENF
                     RRGNLVYSDGRFRFQHRPYWPCRLRRAALAEFSAAPRAAQRRLVRDAVLDGQRSPEGT
                     HAAATMLLRPLLRPSTVRRLSAAPAAQAEQEERHAAAARLSVQAGDLERAERCCYQAA
                     ILMQKEHRAAAALRLIQTVLDYAALVRREWETVSLMKLRGDLLKTSGDTEAAHRTYLQ
                     AAGLYDGRPPDPLLGRIYNSIGDLYKMKQKFAEGIEALERARQIYEAIGDELELSHTV
                     NNMGNMHFVTGRSGEALRHYRRALRIQRHLGSSSEVASTLNNMGAAAGVGGRLRRAAT
                     LFRLSLRLQRELGDLGEIARTLNNLGYLLFLLGEKGQSIQLLAESLELNRRIGNRKEI
                     LFNLDNLTNVTIGAGRLRESAEYLREGMAIAAEVQDIPHQVIFSRNASVVMRRMGRIR
                     EALEYQRAAEAGADRTDDREERIRLQINAASLRAAVGDDDQALALAGSAAREAERIAC
                     KAELLNALLLVTRLADEPAREDEAIALVRDLDLTRERLLVRLNRAERRLHSGRAADAA
                     PVVEEFLLEADLGEDLEQPRLLLVRAEYGMAVGRVMDAGADAQRAAALAGQCGLAPEK
                     MSALALLAQRHLAEGEYETAFAVSRQALAIGKAIAESIPDPADRRLYQSARPIQAIAA
                     TVKQIQERIGQKRAGRDLTPAR"
BASE COUNT          533 a         1102 c         1169 g          484 t
ORIGIN      
        1 ccaccgtgag cgacaacggc accggcatgt cggacgagac gcgcagcaag atcttcaccc
       61 tgcacttcac gaccaagaag gggggccacg gcctggggtt gtccaactgc aagaagatca
      121 tccaggcgca ccacggcgac ctgctggttg actcgacgcc cggcgaaggc accgaattca
      181 gagtgattct cccccgcttt cagccggaga agccgaagag cgacaaaaaa tgacttcgcc
      241 cgcccttccg ccggggtatt gccggacggc gcctctgggc cggggaggga cagcggaggt
      301 agtgcgcgcg ttcagcgccg cgctggggcg cgaggtggcg ctcaagatcc ccttggccga
      361 tgcggatccg tcaggcgcgg cgccgtttgc ggcgctcgtg cggcgggagc gtcttttgat
      421 cggcgggcgg cggttccccg gactggtgcg gctgatcgaa gccggcgagg cgcccccgta
      481 cgtggccatg gaaatctgcc ccggaccgac gctcgacagg ctgggcaaaa tcgactccct
      541 ctccctcgcc ctgaatctcc tggcggcgct caccgccgac ctcgaatacc tccgggccgc
      601 gggaattgtg catggcgatc tcaaaccgca gaacgttttc ctccccgccg cttgggaggg
      661 gcttgacggc cggcaactgt tctgggtcaa gctctccgac ttctcgctcg ggcgcgccga
      721 cggggagtcc gacagcgacc gcgccggact ggggacagtc ggctacctgg cgccggagac
      781 gatcgtcggc ggcgccgccg agcaccagag cgacctgttc gccctcgggg tcaccgccta
      841 ccagatgctg accgggctcc acccgttcat gaacggcgag acggagccgg ccgcgatcaa
      901 cagccgctgc cgcgaggagg agccgcctca ggtcgacacc ctccgccccg atgtcccagc
      961 cgctctgtcc cgcctgattg cccggctgct tcacaaggat cgcgctcaac ggccggtctc
     1021 ggcctgggag gtatgcctcg agttggagcg ggtgggggcc gagtatccgt ttcgccgggc
     1081 gctccagccc ggccactttc tgcgcgcggg cgcgtcatgg gaagatgcgc gggacggcct
     1141 gccggtggag tggggggatt acacggacta cctggccgct ctctcctggg ggtcgcccgc
     1201 gcaacttcgg atgattctga ccgagaattt ccggagggga aacctggtct attccgacgg
     1261 tcggtttcga tttcagcacc gcccctactg gccctgccgg ctgcgccggg cggcgttggc
     1321 ggaattttcc gccgccccga gagcggcgca gcggcgcctg gtgcgggatg cggtcctcga
     1381 cgggcagaga tccccggagg gaacgcacgc ggccgcaacc atgctcctgc ggccgttgct
     1441 gcggccgtcg accgtgcggc ggctgtcggc ggcgccggcg gcgcaggccg aacaggagga
     1501 gcggcacgcg gcagcggcac ggctttctgt gcaggcggga gatctggaac gggcggaacg
     1561 gtgctgctac caggccgcca tcctcatgca gaaggagcac cgggcggcgg ccgcgctccg
     1621 gctgatccag acggttctcg attacgccgc gctggtgcgg cgcgagtggg agacggtgtc
     1681 gctgatgaag ctgcggggcg atctgctgaa gacctcgggc gacaccgagg cggcgcaccg
     1741 cacctacctt caggcggccg gcctctatga cggccgcccg cccgacccgc tgctggggcg
     1801 gatctacaat tcgatcggcg atctgtacaa gatgaagcag aagttcgccg aggggatcga
     1861 ggcgctggag cgggcgcggc agatatatga ggcgatcggc gacgagctgg agttgtcgca
     1921 cacggtcaac aatatgggca acatgcattt cgtgaccggc cgcagcggcg aggccctgcg
     1981 ccactaccgc cgggcgctgc gcattcagcg ccacctcggg tcctcctcgg aggtcgcctc
     2041 cacgttgaac aacatgggag cggccgccgg tgtcgggggg cgcctgcgcc gcgcagcgac
     2101 cctcttccgg ctgtccctgc ggctccaacg ggaactggga gacctcggtg aaatcgcccg
     2161 caccctcaac aatctcggct atctcctgtt ccttctcggc gagaaggggc agtcgatcca
     2221 actgctcgcc gagtcgctcg aactcaaccg ccgcatcggc aaccggaaag agatcctgtt
     2281 caatctggac aacctgacca atgtcacgat cggggccggg cgactgcgcg agtcggcgga
     2341 atatctgcgg gaggggatgg cgattgcggc cgaggttcag gatatccccc accaggtcat
     2401 tttcagccgc aacgcctcgg tcgtcatgcg gcgcatgggg cgtatccgcg aagcgctcga
     2461 gtaccagcgc gcggccgagg ccggggcgga tcggaccgac gaccgggagg agagaatccg
     2521 tctgcagatc aacgcggcga gtctgcgcgc ggcggtcggg gatgacgacc aggccctggc
     2581 gctcgccggg tccgcggcgc gcgaggccga gcggatcgcg tgcaaggcag agcttctgaa
     2641 cgccctcctg ctcgtcacgc ggctggccga tgagccggcg cgcgaggatg aggcgatcgc
     2701 cctcgtgcgc gatctcgacc tcacccgcga gcgcctgctg gtccggctca accgggccga
     2761 gcgccgcctg cacagcggcc gggcggccga tgcggcgccg gtggtggagg agtttctcct
     2821 ggaggccgac ctcggggagg acctggagca gccgcgcctg ctgctggtgc gcgccgaata
     2881 cgggatggcg gtcggccggg taatggacgc cggggcggac gcgcagcgcg ccgcggcgct
     2941 ggccggccag tgcggtctgg ccccggaaaa aatgtcggcc ctggctctgc tggcccagcg
     3001 acatctggcg gagggggagt atgagacggc gttcgcggtc agccgccagg cactcgcgat
     3061 cggcaaggcg atcgccgaga gcatccccga ccccgccgac cggcgcctct accagagcgc
     3121 gcggccgatc caggcgatag cagcaaccgt gaagcagata caggaacgga tcgggcaaaa
     3181 aagagcgggc agggacttga cccctgcccg ctagttcata cgaccagcgg gtcgactcag
     3241 ccgccgccac cgggaccaag caggttgatc ccctggccgc ccacgctg
//
LOCUS       DAOOKS010000149         3280 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_35446_length_3280_cov_3.570853, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000149 DAOOKS010000000
VERSION     DAOOKS010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35446_length_3280_cov_3.570853"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..915
                     /locus_tag="PK112_03295"
     CDS             157..915
                     /locus_tag="PK112_03295"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HPC11108.1"
                     /translation="MVKQTIFVALNAIIAFAIGFGLWYGVFRTSETPIVHSIYQGGPL
                     VVLLIMIFIMLIVFVIERYLSLYKVAKGRGSVQVFFKKLVTLIQNDDFDGALAACDKQ
                     RGTTANVLRAGVERYREIKGNVEYTPEKRIQLTQSAIEEANALEGPLMERNLIALSTI
                     ASIATMVGLLGTTIGMIRAFAATGNVEGGVIDAQQLAVGISEALVNTAGGLLSGILGI
                     FFYNFFVNKVDAFNYTTDEATFEVMQILKQKEGI"
     gene            919..1413
                     /locus_tag="PK112_03300"
     CDS             919..1413
                     /locus_tag="PK112_03300"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HPC11109.1"
                     /translation="MAKKRRVAIRIDMTPMVDIAFLLLIFYMATTQFKPPEARAVELP
                     KSHSQIELPDKDIINITVSKLDSIYVDYVEKATITIEGQDVTTTVRRVRTADKYNVAQ
                     EILKARAKNIKSLVVIKADQAASFGIMQDIMKSMQENNLERFLIITDQETEAGGMERV
                     IFEG"
     gene            1473..2126
                     /locus_tag="PK112_03305"
     CDS             1473..2126
                     /locus_tag="PK112_03305"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HPC11110.1"
                     /translation="MAGEVVQREAKGKKKGLRRQKRRIAIRIDMTPMVDIAFLLLIFY
                     MVSTVFSMPQAMEINLPPAEQMDQEIEVKESNLLTIRVDGQGRYWWNLKTPTPDNLPL
                     LLPPDPQRPDTIPYRLWEDTLRGLLVNQNRLNPKLNTLILIHKDGSYADMVDILDEID
                     LIERSWNEYQAKKLRKKLDELSKDEKFSYRYAIGEWMARDDKVQEAAEAQARLGGLL"
     gene            2126..2905
                     /locus_tag="PK112_03310"
     CDS             2126..2905
                     /locus_tag="PK112_03310"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HPC11111.1"
                     /translation="MAETTRSDKGPLAMAAGGRGWSVLYSPYGAFEMKAKYQRNFLFG
                     TLITTSVVALIVIVAAIISSLPEEDYSDVPQVVIKTVADLGPPPSIARKPPQVQVAQP
                     QVAAPKVGIPKPVADEEVVDEDVVIATQEELAEITAPSISEAAGSGDIVVDIQEEDFI
                     PAPEDFVPVEVQPEVISEVKPDYPPLARQAGITGDVYVKALVDEQGNVMKAILAKSSG
                     TQSLDDAALEAAHKRKYRPGIQNNRPIKVWVTYKVEFRLNE"
BASE COUNT          679 a          958 c         1071 g          572 t
ORIGIN      
        1 cagtctcggc gacagctacg ggccgggcgt gctgctgggg gtgctgtcct ggggattgat
       61 cgtcgcgctg gcggcctcga tcctggcggc ggcgaagggt gcggaaatat aagtcggttg
      121 gtgagtgtgc aataaaagga aacggaggcc atttcagtgg ttaaacagac aatcttcgtc
      181 gcgctcaacg ctatcatagc gttcgccatc gggttcgggc tgtggtacgg ggtgttccgg
      241 acatcggaaa cgccgattgt ccactcgatc taccagggcg ggccgctcgt cgttctcctg
      301 atcatgattt tcatcatgct catcgtgttc gtgattgagc gctacctctc gctctataag
      361 gtggccaagg gccgcgggtc ggtccaggtg tttttcaaga agctggtcac cctgattcag
      421 aacgacgatt tcgacggtgc gctggcggcc tgcgacaagc agcgcggcac caccgcgaac
      481 gtgcttcgcg ccggcgtcga acgctaccgc gagatcaagg gcaacgtcga gtatacgccg
      541 gagaagcgca tccagctcac ccagtcggcg atcgaagagg ccaacgccct cgagggaccg
      601 ctcatggagc gtaacctgat cgcgctgtcg acgattgcct cgatcgcgac catggtgggt
      661 ctgctgggga cgacgatcgg gatgatccgg gcgtttgccg cgacggggaa tgtcgagggc
      721 ggcgtgatcg acgcgcagca gctggcggtg gggatttccg aggccctggt gaacacggcc
      781 ggcgggctgc tctccggtat cctcgggatc ttcttctaca acttcttcgt caacaaggtc
      841 gacgcgttca actacacgac ggacgaggcg acttttgaag tgatgcagat tctcaagcag
      901 aaagagggaa tctaaggtat ggcgaagaaa cggcgggtcg ctatccgcat cgacatgacg
      961 ccgatggtgg atatcgcctt tctgctgttg attttctata tggcgacgac gcagttcaag
     1021 ccgccggaag cccgcgcggt ggagctgccg aagtcgcact cgcagatcga gctgccggac
     1081 aaggacatca tcaacattac cgtgtccaag ctggactcga tctacgtcga ctacgtggag
     1141 aaggcgacca tcacgatcga gggtcaggac gtcacgacga cggtgcgccg ggtgcggacc
     1201 gccgacaaat acaacgtggc gcaggagatc ctcaaggcgc gcgccaagaa catcaagtcg
     1261 ctggtggtga tcaaagccga ccaggcggcc agtttcggca tcatgcagga catcatgaag
     1321 tccatgcagg agaacaacct cgagcggttc ctcatcatta cggaccagga gacggaagcc
     1381 ggcgggatgg agcgcgtcat ctttgaaggc tgagccaagc ggcaccggaa cggccgccgg
     1441 atgcggcgcc ggaaggagag tgaagagaca atatggccgg tgaagtagta caacgggaag
     1501 ccaagggcaa gaagaaaggt ctgcggcggc aaaaacgccg gatcgcgatc cgcatcgaca
     1561 tgacgccgat ggtggacatc gccttcctgc tcctgatctt ctacatggtc tcgaccgtgt
     1621 tctcgatgcc gcaggcgatg gagatcaacc tcccgcccgc ggagcagatg gatcaggaga
     1681 tcgaggtgaa ggagtccaac ctgctgacga tccgggtcga cggccagggt cgctactggt
     1741 ggaatctcaa gacgccgacg cccgacaacc tgcccctctt gctgccgccg gatccccagc
     1801 gccccgacac gatcccctac cgcctgtggg aggacacgct gcgcgggctg ctggtgaacc
     1861 agaaccgatt gaaccccaaa ctgaacacgc tgatcctgat ccataaagac ggcagctacg
     1921 ctgacatggt ggacattctc gacgaaatcg acctgatcga acgttcctgg aacgagtatc
     1981 aggcgaagaa gctccggaag aaactggacg agctgagcaa ggacgagaag ttctcctacc
     2041 ggtacgcgat tggcgagtgg atggcgcgcg acgacaaagt gcaggaagcg gccgaggcgc
     2101 aggcccggtt aggagggctg ttgtaatggc agagacaacg cggtccgaca aggggccttt
     2161 ggcgatggcg gccggcgggc gcgggtggag cgtgctctac tctccctacg gcgccttcga
     2221 gatgaaggcc aagtaccagc ggaatttcct cttcggtacg ctcatcacca cctcggtggt
     2281 ggcgctcatc gtgattgtcg cggcgatcat ttcgagcctg cccgaggagg attactccga
     2341 cgtgccgcag gtggtgatca agaccgtggc cgatctgggt ccgcctccct cgatcgcccg
     2401 caagcctccg caggtccagg tcgcccagcc gcaggtggcc gcccctaaag tgggtattcc
     2461 caagccggtg gccgacgagg aagtggtcga cgaggacgtg gtgatcgcga cccaggaaga
     2521 gctggcggaa atcaccgccc cgagcatctc ggaagcggcc ggttccggcg acatcgtcgt
     2581 cgacatccag gaagaggatt tcatccccgc cccggaggac tttgtcccgg tcgaggtcca
     2641 gccggaggtc atctccgagg tgaaaccgga ttacccgccg ctcgcccggc aggccgggat
     2701 caccggcgac gtctacgtca aggcgctggt ggatgagcag gggaatgtca tgaaggcgat
     2761 tctcgccaag tcctcgggca cgcagtcgct ggacgatgcg gcgctcgagg cggcccacaa
     2821 acgcaaatac cggccgggga tccagaacaa ccggccgatc aaggtctggg taacgtacaa
     2881 agttgagttc cggctcaacg agtagccgac atcggttttc actctccgtt tacagggcgc
     2941 ccgccggctt cggcgggcgc ttctcttttg gccattttcc gctccccgat tgccccggct
     3001 tctccgtcac tctagtgagg gcgtccccgc agacccggcc gcccgtttca gggaagaatc
     3061 gccggctcgg cgggtactct cgacagaggc gacggaggtt cggtattcga agtgagagga
     3121 gccaagtatg gcgaaccagg caaacccgct gtacgcgccc tacggcgcat acgcgatgaa
     3181 agcggcctac cagaaaaacc tggcggcggc gaacctgctg acggcgctgg cggtcggcgc
     3241 ggcggtcctg gccggaacgc tctggccggc gaacgaagag
//
LOCUS       DAOOKS010000150         3271 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_35594_length_3271_cov_3.279540, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000150 DAOOKS010000000
VERSION     DAOOKS010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35594_length_3271_cov_3.279540"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="PK112_03315"
     CDS             <1..423
                     /locus_tag="PK112_03315"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPC11112.1"
                     /translation="DLAEDLPLIQANASQLQQVFTNLIINAMHASRSDSQIRIESRYS
                     PAVGEFGGAVELRFIDQGHGIPPEIINKIFEPFFTTKEVGKGTGLGLSVSYGIVKEHG
                     GEIKVDSAVGKGSTFTLIFPVQKHPVDADNSLKSGIGV"
     gene            451..822
                     /locus_tag="PK112_03320"
     CDS             451..822
                     /locus_tag="PK112_03320"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPC11113.1"
                     /translation="MDESVRRSVLVVDDEEIIRSFLFEVLSEEYDVSLACDGDEAIEQ
                     IRKRRYDVIITDLKMPRVSGEEVVKFACDRDPQSKVIVISGFSSLYTVSQSINNGACA
                     FLSKPFSIKELMRTVTNALCA"
     gene            833..1201
                     /locus_tag="PK112_03325"
     CDS             833..1201
                     /locus_tag="PK112_03325"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPC11114.1"
                     /translation="MARILIIDDSEVIRNLLEEYLTDAGYEVETACDGREGIDRALGG
                     DFDVAFCDIHMPHKNGYQVFREVRRRKPGMAFIMTDSLPDQLAEMAQNEGAHCCLTKP
                     FDLNQVRRVLETLLAKARTV"
     gene            1198..2919
                     /locus_tag="PK112_03330"
     CDS             1198..2919
                     /locus_tag="PK112_03330"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPC11115.1"
                     /translation="MSGTETRSINVLVVDDETIVLSLVRDALEDEGYEITTAVGGDEA
                     LQALDSRPVDLLITDIRMPHMDGTELVRRARERNPHIGVIYMTGYANLNSAKDAIKQG
                     AFDYILKPFELTEIRQAVHKAAGKLRERAGQDSGEQLERLSDLNQMLVTVGDRKSLAS
                     VSLRFAMMHFDATQGALLHWDRERTAVETITIHGDQLQETALDSPAVREFIRAIDPAS
                     FREPVIVTSPERHPLAGPAAAPEIRAALFPSWLTEGSAMVVMPINRANILYGIIMIRC
                     SPDATALTGTDTRLLNIAAGQLAVSLENLFLLEETQTAYARLKEMQDETIQLEKLATR
                     GEMSAEIGHELNNFLGVVAGNISLLDHQLKKSGAATAEKYMSAIQTNIEKMKQFTSNL
                     MDLTPIASKKETVYFDQVLAEVIDYLTPQKRFRGVSIDHAVDHESIPFEADSIHIQQL
                     LYNLFNNAADATRGCARREIVSRVEYRPGEPHFRFVIRDTGVGIEPELLAKAFEQKFT
                     TKPDGHGFGLLVCKRIIDAHSGRLHVDSTPGEGTAISIEFPVAGAADSVDATAGQFAI
                     TDKIPVG"
     gene            complement(3041..>3271)
                     /locus_tag="PK112_03335"
     CDS             complement(3041..>3271)
                     /locus_tag="PK112_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11116.1"
                     /translation="RYRLLSAATAAGEQVRVMQPAGPGRFVDTLQMPGTAGRLWEFLF
                     MQDFEYVPIPGTQPPDSQLTWTGWCVPYRIVP"
BASE COUNT          657 a         1076 c         1008 g          530 t
ORIGIN      
        1 gacctggccg aagatctgcc gttgatccag gccaacgcca gccagttgca gcaggtcttc
       61 acgaacctga tcatcaacgc catgcacgcc tcccggtccg attcgcagat ccggatcgag
      121 tcccgttaca gcccggccgt cggggagttc ggcggcgccg tcgagctccg cttcatcgac
      181 cagggccacg gcatcccccc cgaaatcatc aacaagatct tcgagccgtt cttcaccacc
      241 aaggaagtgg gcaaggggac cggcctcggc ctctccgtga gctacgggat cgtcaaggag
      301 catggggggg aaatcaaggt cgactcggcg gtcggaaaag gctccacttt cactctgata
      361 ttccccgttc agaaacaccc ggtcgacgcc gataattctc taaagagcgg gataggagtg
      421 taagtacttc cgaacttgcg ggatgtttca atggatgaat cggtcagacg ttccgtcctc
      481 gtcgtggacg acgaggaaat cattcgcagt tttctgtttg aagtgctcag cgaggagtac
      541 gacgtcagcc tggcctgcga cggcgacgag gcaatcgagc agatccggaa gaggcgctac
      601 gacgtcatca ttacggatct caagatgccc cgggtgtcgg gcgaggaggt cgtcaagttc
      661 gcctgcgaca gggatccgca gtcgaaagtc attgtgattt cgggcttttc aagcctatac
      721 acggtcagtc aatccataaa caacggcgcc tgcgcattcc tctcgaagcc tttttccatc
      781 aaggaactga tgcggacggt cacgaatgcc ctgtgcgcct agggacacgg caatggcacg
      841 aatactgatc atcgacgatt ccgaggttat tcgcaacctg ctggaagagt atttgaccga
      901 cgccgggtac gaggtcgaga cggcctgcga cggccgggag gggatcgacc gggctctggg
      961 gggggatttc gacgtggctt tctgcgacat ccacatgccg cacaagaacg ggtaccaggt
     1021 gttccgggag gtgaggcggc gcaagcccgg gatggcgttc atcatgaccg actctctgcc
     1081 cgatcaactg gccgaaatgg cccagaacga aggcgcccac tgctgcctga ccaaaccgtt
     1141 tgatctgaac caggtccgcc gcgtcctgga gacgctgctg gccaaagcgc gcacggtatg
     1201 agcggcacgg aaactcgcag tataaatgtc ctcgtggtcg acgacgagac catcgtcctg
     1261 tccctcgtgc gcgatgcgct cgaggacgag gggtatgaga ttacgaccgc ggtcgggggg
     1321 gacgaggccc tgcaggcgct cgactcccgg cccgtcgacc ttctcatcac cgatatccgc
     1381 atgccgcaca tggacggtac cgagctggtg cgccgcgccc gcgagcgcaa cccccacatc
     1441 ggcgtcatct acatgacggg ctacgccaat ctgaattcgg ccaaagacgc gatcaaacag
     1501 ggcgccttcg actatatcct gaaaccgttc gagctgaccg aaatccgcca ggcggtgcac
     1561 aaggccgcgg ggaaactccg cgagcgggcc gggcaggact cgggcgaaca gctcgaacgc
     1621 ctctccgacc tcaaccagat gctcgtcacg gtcggcgacc gcaagtcgct ggcgagcgtc
     1681 tcccttcgtt tcgccatgat gcatttcgac gccacccagg gggccctcct ccactgggac
     1741 cgggagcgga ccgcggtgga gacgatcacg attcacggcg accagcttca ggagacggcg
     1801 ctggacagcc cggcggtgcg ggagttcatc cgcgccatcg acccggcctc gttccgggag
     1861 ccggtcattg tcacgtcgcc ggagcggcac ccgctggccg gcccggcggc ggccccggag
     1921 atccgcgcgg ccctgttccc ctcctggctc accgaaggga gcgccatggt cgtcatgccg
     1981 atcaaccgcg cgaacattct gtatggcatc atcatgatca ggtgctcccc cgacgccact
     2041 gcgctcaccg ggaccgacac ccggcttttg aacatcgccg ccggccaact ggccgtttcg
     2101 ctggagaatc tgtttctgct cgaggagacg cagaccgcct acgcgcggct caaggagatg
     2161 caggacgaga ccatccagct ggagaaactg gccacccgcg gggagatgtc ggcggagatc
     2221 ggccatgagc tgaacaactt cctcggggtc gtcgccggca acatttcgct gctggaccac
     2281 cagctgaaga agtcgggcgc ggccacggcc gaaaagtaca tgagcgccat ccagaccaac
     2341 atcgagaaga tgaagcagtt cacgagcaac ctcatggacc tcaccccgat cgcctcgaaa
     2401 aaggaaacgg tctacttcga ccaggtcctg gccgaagtca tcgactacct cacgccgcag
     2461 aagcgtttcc ggggagtgtc catcgaccac gccgtcgacc acgagtcaat tccgttcgag
     2521 gccgacagca tccacatcca gcagttgctc tacaacctgt tcaacaatgc ggccgacgcc
     2581 acccgcgggt gcgcgcgccg ggagattgtg tcgcgcgtcg agtaccggcc cggcgaaccg
     2641 cacttccgct tcgtcatccg cgacacgggc gtcggcatcg aaccggaact tctcgccaag
     2701 gcgttcgagc agaagttcac gaccaagccc gacgggcacg gattcggcct gctcgtctgc
     2761 aagcggatca tcgacgccca cagcggccgc ctccacgtcg actccacacc cggcgagggg
     2821 accgcgatca gcattgaatt cccggtggcc ggtgccgcgg acagcgtcga cgcgacggcc
     2881 gggcagttcg ccatcaccga caagatcccc gtcggctgac ccgcctctcc ctcacaacgc
     2941 cgcggaacga aagcggccgc gcccccgggg accgcatgcc agccggagga ggccagccac
     3001 ggaggcccgg cgcccctgcg ccgccgcgtc tcgggagctc tcaggggaca atccggtagg
     3061 ggacgcacca gccggtccag gtcagttgcg agtcgggcgg ctgggttccc ggaatgggga
     3121 cgtattcgaa atcctgcatg aacaggaact cccacaaacg cccggccgtc cccggcatct
     3181 ggagggtgtc cacgaagcgc ccgggaccgg ccggctgcat cacgcgcact tgctcgcccg
     3241 cggccgtcgc ggccgagaga agccggtagc g
//
LOCUS       DAOOKS010000151         3267 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_35670_length_3267_cov_3.122976, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000151 DAOOKS010000000
VERSION     DAOOKS010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35670_length_3267_cov_3.122976"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            5..172
                     /locus_tag="PK112_03340"
     CDS             5..172
                     /locus_tag="PK112_03340"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPC11117.1"
                     /translation="MSEKRANRVRDFLAAQGVAESRMSVNGRGETGFVASNETAEGRA
                     LNRRIEIVFYK"
     gene            178..732
                     /locus_tag="PK112_03345"
     CDS             178..732
                     /locus_tag="PK112_03345"
                     /inference="COORDINATES: protein motif:HMM:NF024952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HPC11118.1"
                     /translation="MYDHYLEQNIEDRRSARFALVVFLPPELDDHLAPLRERYDPLWS
                     LIPAHATIVFPFESRHSLDGLTGLIQEELAHEPVIPIRLGTIHDFYPRYPVICWKIQK
                     NEQLTGIYYRMYARLGMPIPFKEYQPHVTVAREISDHRVMLVKEKIVPYLSEEQFVAR
                     SIELVTPLPGNRWVSVRTFPLAGA"
     gene            complement(830..1354)
                     /locus_tag="PK112_03350"
     CDS             complement(830..1354)
                     /locus_tag="PK112_03350"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HPC11119.1"
                     /translation="MRKVSVSALILVSFMMLAFLAYAQETAEKKAFEYIGPAKCKMCH
                     KDVHAAWETTAHAKAFASLSAEEQKKAECNVCHMTGANAKGEAIENVTCESCHGPGSA
                     YSKPTIMTKKWSEDPATYKAKAIEAGLIYPDSALCATKCHKKEGNPNFKPFSWDKMSK
                     LVHPVAATPAEEKK"
     gene            1670..2836
                     /locus_tag="PK112_03355"
     CDS             1670..2836
                     /locus_tag="PK112_03355"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPC11120.1"
                     /translation="MRRLAEFARQFVKAGMDVSVLTAVPNYPDGIVPEKYRGKFFYRE
                     TVDGVKVYRSYVMPASNRFPGRRMVGFVIFLITALINSFRIKGNFDLILASTPPVTTP
                     VIGWVLSRVRRAKLIIEIRDLQPESSEEFGNLRPSLFTRMLKKFMHMLYRRADHVVTA
                     TDGIANYLTGLGFAKERVTTIKSGVGREFLSSDSNGIRRKFGWDEKFLVLYAGTLGWA
                     HSLETVIEAARHLVDQPDIHFVFAGEGQKRQALEGMVRDYGLKNVTFIGLQSLETIPY
                     FLRASDVLVESLKEVPITKGTFPCKLFEYMASGRPIIFGSSGGEAVSELEKAGGALWF
                     STDRPERLSELILQIKGGEIDGGRLGAAYQAHVDRYHRREVWADRYLEVIRSLS"
     gene            2924..>3267
                     /locus_tag="PK112_03360"
     CDS             2924..>3267
                     /locus_tag="PK112_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPC11121.1"
                     /translation="MGLSSRPAAAPAQRLTVADLPGLLIGGETVAPTLRGPQRYVNFD
                     NAASTPTFRPVAEAVMEFLQWYANVHRGTGFKSQLASWAFEEARDIIAGFIRADLQRQ
                     VVIFTKNTTEAI"
BASE COUNT          571 a          892 c         1135 g          669 t
ORIGIN      
        1 gagcctgtcg gagaagcggg ccaaccgggt gcgggatttt ctcgcggccc agggggtggc
       61 ggagagtcgc atgagcgtca acggccgggg ggagaccggc tttgtcgcct cgaacgagac
      121 ggccgagggg cgggcgttga accggagaat tgagattgtc ttctacaagt agacggtatg
      181 tacgatcatt accttgagca gaacatcgaa gaccgacgca gcgcgcggtt cgccctggtc
      241 gtgttcctcc ccccggagct ggacgaccat ctcgccccgc tgcgggagcg gtacgatccg
      301 ctgtggagcc tgatccccgc gcacgcgacg atcgtctttc cgttcgagtc gcgccactcg
      361 ctggacggac tgaccggctt gatccaggag gagctggcgc acgagccggt gattccgatc
      421 cggctgggga cgatccacga tttctacccg cggtatccgg tgatctgctg gaagatccag
      481 aagaacgagc agctcaccgg catttactac cgcatgtacg cgcggctggg gatgccgatt
      541 ccgttcaaag agtatcagcc gcacgtgacg gtggcgcggg aaatctcgga tcaccgggtg
      601 atgctggtga aggagaagat cgtgccgtac ctgtcggagg agcagttcgt cgcccggagt
      661 atcgagctgg tgacgccgct gccggggaac cggtgggtct cggtgcggac ttttccgctg
      721 gccggggcgt gaggcgccgg ggcgaggccc gggagaaagg caatgagaag gcgactcgcc
      781 ggggggcgag tcgccttcat cgttaactca acagccgatc ggaagcggat tacttcttct
      841 cttcagccgg cgtggcggcc accgggtgaa ccagcttgga catcttatcc cagctgaacg
      901 gcttgaagtt cgggttgcct tccttcttgt gacacttggt ggcgcagagg gcggagtccg
      961 ggtagatgag gccggcttcg atcgccttgg ccttataggt ggccgggtcc tcgctccact
     1021 tcttggtcat gatggtcggt ttggagtagg ccgagcccgg gccgtggcag gactcgcagg
     1081 tgacgttttc gatcgcctcg cccttggcgt tggcgccggt catgtggcag acgttgcact
     1141 cggccttctt ctgctcctca gcggagaggg aggcaaaggc tttggcgtgg gcggtcgttt
     1201 cccaggcggc gtggacgtcc ttgtggcaca tcttgcactt ggccgggccg atgtactcga
     1261 aggctttctt ttcggcggtt tcctgggcat aggccaggaa ggcgagcatc atgaaagaca
     1321 ccaggatcag agcgctaaca gagaccttac gcatttgtgc ttctcccttg gttgggttcc
     1381 gtttcccagt ttatacacca atcaatacta ttcggatcgt gatttttgtc acatgggcga
     1441 aaatatcatg tgaatgtcat gctgtcaagt ttttcggatg aatttggtcg gggaatttcc
     1501 gatacatgac gggggcggcg gccggggttg gaggggggtt cctgtgccga agggccaagc
     1561 tggtggagaa aaaaaacttg ccaggttctc caaaaagctg tagactgcgc cgggttagtc
     1621 tatgaaagtc ctgattgtca cacaacacta tccgcccgaa cgcggggctg tgcgacggtt
     1681 ggcggagttt gcccggcagt ttgtcaaggc ggggatggac gtgtccgtgc tcacggccgt
     1741 cccgaattat ccggacggca tcgtccccga gaagtaccgg gggaagtttt tctaccggga
     1801 gaccgtcgac ggggtcaagg tctaccggag ctacgtgatg ccggcctcaa accggtttcc
     1861 gggccggcgg atggtgggat ttgtcatttt cctcatcacc gccctgatca acagcttccg
     1921 gatcaagggg aatttcgacc tgatcctggc ctcgacgccg cctgtgacca cgccggtgat
     1981 cgggtgggtg ctctcgcggg tgcggcgggc gaaactgatc atcgagatcc gggacctgca
     2041 gccggagtcg agcgaggagt tcggcaatct gcggccgtcg ctcttcacgc ggatgctcaa
     2101 gaagttcatg catatgctct accgtcgggc ggaccatgtg gtgacggcca cggacgggat
     2161 tgccaactac ctgaccgggc tgggttttgc caaagagcgg gtgaccacga tcaagtcggg
     2221 ggttgggcgc gagttcctga gcagcgactc gaacggtatc cggcggaagt tcgggtggga
     2281 tgagaagttt cttgtgctct acgcggggac gctgggctgg gcgcattcgc tggagacggt
     2341 gatcgaggcg gcccggcacc tggtcgacca gcccgacatt catttcgtgt tcgccgggga
     2401 ggggcagaag cggcaggcgc tcgaggggat ggtgcgggac tacggcttga agaacgtgac
     2461 ctttatcggt ttgcagtcgc tggagacgat cccctatttc ctgcgggcct cggacgtgct
     2521 cgtggagagc ctcaaggagg tgccgatcac gaaggggacg ttcccgtgca agctgttcga
     2581 atacatggcg tcgggccgcc cgatcatttt cggctcgagc gggggggagg cggtgtcgga
     2641 gctggagaag gcgggcgggg cgctctggtt ctccacggat cgtccggagc ggttgtccga
     2701 gttgatcctc cagatcaagg ggggggagat cgacggcggc cggctcgggg cggcctacca
     2761 ggcccatgtc gaccggtatc accgccgcga ggtgtgggcg gaccgctacc ttgaggtgat
     2821 ccgcagtctc tcctgacatt tcccgtccgg tagcgaattc tctccccgtt ccgtcgcggt
     2881 ataatgaact aatcggacct atttttatca gaggatgcac accatggggc ttagttcccg
     2941 tccggcggcc gcgccggcgc aacggctgac ggtcgccgac cttcccgggc tgttgatcgg
     3001 gggggagacg gtcgcgccga ctttgcgggg gccgcagcgg tatgtcaatt tcgacaacgc
     3061 ggcctcaacg ccgacgtttc ggccggtggc cgaggcggtg atggagtttc tgcagtggta
     3121 cgccaacgtg caccggggca cggggttcaa atcgcaattg gcgagctggg cgttcgagga
     3181 agcgcgggac atcatcgcag gcttcatccg ggccgacctg cagcggcagg tggtcatttt
     3241 caccaagaac acgaccgagg cgatcaa
//
LOCUS       DAOOKS010000152         3266 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_35681_length_3266_cov_3.785737, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000152 DAOOKS010000000
VERSION     DAOOKS010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35681_length_3266_cov_3.785737"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            144..1193
                     /locus_tag="PK112_03365"
     CDS             144..1193
                     /locus_tag="PK112_03365"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HPC11122.1"
                     /translation="MPPAVEREPAPAAPESKTPAPPPPAAPAPRPVSAPAPPPAAPPR
                     PTGPAPASPSVRRLARELGLDISAVSGTGPGGRITESDLKVHVRRAAASGRAAPTGSA
                     PAPAGSPDLPDFSRWGEIERADMSTVRRVTAESTARSWAQIPHVTQSDKADITHLEEF
                     LRKNADRVKNAGGKLTVTAVLLKVMGEGLRRFPRFNASLDPANRQIIYKHYVHIGVAV
                     DTDHGLLVPVLRDVDRKNIVTLAVELADLAERARGRRVAPDELEGGTFTISNQGGIGG
                     TDFTPLVLWPQVAILGVSRAAIEPRYIEREFRPRTILPLALSYDHRAVDGADAARFLR
                     WVCDVLESPFAMFLE"
     gene            1229..2662
                     /gene="lpdA"
                     /locus_tag="PK112_03370"
     CDS             1229..2662
                     /gene="lpdA"
                     /locus_tag="PK112_03370"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HPC11123.1"
                     /translation="MNDTTRNKAQVAVIGGGPAGYGAAFLAADLGLDIVLIDPAENPG
                     GVCLYRGCIPSKALLHTARLLAEVQEAKDRGLAFGAPRVNIRKLRSWKDGVVDKLTGG
                     LGQLVKQRKFRHLRGHARFVDPHTLSIREVAGAEYTFAFEHAVIATGSYPATLGPQQP
                     DSPRLLDSTSALELADIPGTLLVIGGGYIGLEIGTIYAALGSRVSLVEMTDGLLPGVD
                     RDLVAILRRRLDRIFDAVMLETTVRELKVQANGVKVAFAGKQAAGKAQLFDRVLVAIG
                     RKPMSAQLGLDAVGVDTDARGFIKVDAQRRTSAPHIFAVGDVAGEPMLTHKGLHEGRV
                     AAEVIAGRKAAFDPAAIPAVVFTDPEVAWAGLTETEAAGKKIEVEITRFPWAASGRAL
                     TLGRTDGATKLVIDPHSQRLLGMGVVGPGAGELIAEGVLAIEMAATVDDLGLSIHPHP
                     TLSETVMEAADLFHGTATDYYRPKKRK"
     gene            complement(2862..>3266)
                     /locus_tag="PK112_03375"
     CDS             complement(2862..>3266)
                     /locus_tag="PK112_03375"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HPC11124.1"
                     /translation="YDLDEDSLRGLAASYTYVMQNLRLIKRPAENRWQPQDKRWRGLA
                     LGEDTQGRALVILTRSAWSMYDLNEFLLSLPIGIVAAQHLEGGSEAQLFVRTADTTFA
                     IVGSYDTSLDDDDGQWAGWPVPNVIGIAPKTR"
BASE COUNT          531 a         1164 c         1075 g          496 t
ORIGIN      
        1 gttcataccg tcggaggctt cgacgaggct ggaggaattc atccgcccga acatagcctt
       61 cggcgcccgt atcggtctct tctgaccagg cgatccgcgg gaggcgccag cctcccgcgg
      121 atcaccgcgc cgccccgccg cccgtgcccc cggcggtcga acgcgaacct gcgcccgccg
      181 ccccggaatc gaagaccccg gctccgccgc cccccgccgc cccggcgccc cgccccgttt
      241 ccgctccggc tccgccgccg gcggcgccgc ctcggccgac cggccccgct ccggcctcgc
      301 cctcggtgcg gcggctcgcc cgcgagctgg gcctggacat ctccgccgtg agcggcaccg
      361 gaccgggcgg ccggatcacc gagagcgacc tcaaggtcca tgtccgacgc gccgccgctt
      421 ccgggcgagc cgcaccgacc gggagcgccc cggctcccgc cgggtcgccg gatctgcccg
      481 acttcagccg gtggggagaa atcgagcggg ccgacatgtc gaccgtccgc cgcgtgaccg
      541 cggaaagcac cgcccggtcg tgggcgcaga ttccgcatgt cactcagtcg gacaaagccg
      601 acatcaccca ccttgaggag ttcctccgca aaaacgccga ccgcgtgaaa aacgccggcg
      661 ggaagctcac cgtaacggcc gttctcctga aagtgatggg ggagggactc cggcggttcc
      721 cccgcttcaa cgccagcctc gatcccgcga accgccagat catctacaag cactacgtcc
      781 acatcggcgt cgccgtcgac accgaccacg gactgctcgt accggtgctg cgcgatgtgg
      841 accggaagaa catcgtcacc ctcgcggtcg aactggccga cctggcggaa cgcgcccgcg
      901 gccggcgagt cgcgcccgac gagctggagg ggggcacgtt cacaatttcc aaccaggggg
      961 ggatcggcgg gaccgatttc acccccctcg tcctctggcc gcaggtagcg attctcggcg
     1021 tcagccgcgc ggccatcgaa ccgcgctata tcgaacgcga gttccggccg cgcacgatcc
     1081 tgccgctggc gctgtcgtac gaccaccgcg ccgtcgacgg cgccgatgcc gcgcgctttc
     1141 tccgctgggt gtgcgacgtg ctcgagagtc cgttcgccat gtttctggag tagtccgcgg
     1201 cgcggccaaa ccggaaggga gacctgacat gaacgatacc actcggaaca aggcgcaggt
     1261 ggcggtgatc ggcggcgggc ccgccggcta cggggccgct ttcctcgccg ccgatctggg
     1321 cctggacatc gtgctgatcg atccggccga aaaccccggc ggcgtctgcc tctaccgcgg
     1381 gtgcattcca tccaaagcgc tcctccacac ggcgcgcctg ttggccgagg tgcaggaggc
     1441 gaaagaccgg ggcctcgcct ttggcgcgcc ccgggtgaac atccggaaac tccgctcctg
     1501 gaaagacggc gtcgtcgata agctcaccgg gggactcggc cagctcgtca aacagcgcaa
     1561 gttccgccac ctccgcggcc acgcccgctt cgtggatccc cacacgctgt cgatcaggga
     1621 agtggccggc gcggaataca ccttcgcctt cgaacacgcc gtcatcgcca ccggctctta
     1681 ccccgccacc ctcggcccgc agcaacccga ttccccccgc ctcctcgact cgacctcggc
     1741 cctcgaactc gcggatattc cgggcacgct gctcgtgatc ggcggcggct atatcggcct
     1801 ggaaatcggc accatctacg ccgccctcgg cagcagggtc agtctggtgg aaatgaccga
     1861 cggcctcctg ccgggagtcg accgcgatct cgtcgccatc ctccgcagac ggctcgaccg
     1921 gatcttcgac gccgtcatgc ttgagacgac cgtcagagaa ctcaaagtgc aggccaacgg
     1981 ggtgaaagtc gcgttcgcgg ggaagcaggc cgccgggaag gcgcagttgt tcgaccgtgt
     2041 gctcgtggcg atcgggcgca agcccatgtc cgcccagctt gggctcgacg cggtgggggt
     2101 cgataccgac gcgcgcgggt tcatcaaggt ggatgcccag cggcgcacca gcgcgccgca
     2161 catcttcgcc gtcggcgacg tcgccggcga accgatgctt acccacaagg ggctgcacga
     2221 agggcgggtc gccgccgagg tgatcgccgg ccgaaaagcc gccttcgacc ccgccgccat
     2281 acccgccgtc gtcttcaccg atcctgaggt cgcctgggcc ggcttgaccg aaaccgaggc
     2341 ggctgggaag aaaatcgagg tcgagatcac gcgcttcccc tgggccgcct cgggccgggc
     2401 tctcacgctc ggccgcaccg acggcgccac aaagctggtc atcgacccgc actctcagcg
     2461 cctcctcggg atgggcgtgg tcggacccgg cgccggcgaa ctgatcgccg agggcgtgct
     2521 ggcgattgag atggcggcca ccgtcgacga cctcggcctg agcattcatc cgcacccgac
     2581 gctgagcgag acggtgatgg aggcggccga cttgttccac ggcaccgcca cggattacta
     2641 ccggccaaag aaaaggaagt aggacaggac gccggcggtc acaacgctct ctccatccgc
     2701 agagacaatt ctgcggatct cttgtaggtc aggacccttg tggtcctgac attcttacta
     2761 tcgatgtaag cagccccgtc aggaccatag aggtcctgac ctgcagaacc ctgtcctgcg
     2821 gaagttggcc ggggggcgct cggcctggcg gcgggggcgg gttatcgggt tttcggggcg
     2881 atgccgatga cgttgggcac cggccagccc gcccactgac cgtcgtcatc gtcgagcgag
     2941 gtgtcgtagc tgccgacgat ggcaaaggtg gtatcggcgg tgcgcacgaa aagctgcgcc
     3001 tcggatccgc cctcgaggtg ctgggcggcg acgattccga tcgggagcga gaggaggaat
     3061 tcgttcagat cgtacatgga ccaggccgac cgggtgagga tcacgagcgc ccgtccctgg
     3121 gtatcctcgc cgagggccag tccgcgccag cgtttgtcct ggggctgcca gcggttttcg
     3181 gcggggcgct tgatcaggcg gaggttctgc atgacgtagg tgtaggaggc ggccagtccc
     3241 cggagggagt cctcatcgag gtcgta
//
LOCUS       DAOOKS010000153         3249 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_35933_length_3249_cov_3.298059, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000153 DAOOKS010000000
VERSION     DAOOKS010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35933_length_3249_cov_3.298059"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            204..1265
                     /locus_tag="PK112_03380"
     CDS             204..1265
                     /locus_tag="PK112_03380"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPC11125.1"
                     /translation="MPGHIARTTGRIAALRTAGWIAAARSTGRIAAVRTAGRSTAART
                     VGWIAAAAAAAVLGCSGADDTAKVKQNPASEGAVTIGVSLLTRTHPFYQELEEGLKEA
                     AGAAGFQVRITAGEFDVARQKDQLQDFLTQRVDAIIVCPCDSRSIGTAIKAANEAGIP
                     VFTADIACLAEGVAIVTHVASDNVEGGRLAARALAEAIHGAGAVAIIDHPEVESVIQR
                     VKGFEEEIAKYPDITIVAKLSGHGVKDQAFRTAEDILQAHPDLAGFFGINDDSALGAL
                     AAIEKAGKLGRVKIVGFDAVPEARQAIEAGKIHADVIQQPRKIGQRTVEAVQTYLSGG
                     AVEPQILIPCDLFAKPAAQ"
     gene            1287..2795
                     /locus_tag="PK112_03385"
     CDS             1287..2795
                     /locus_tag="PK112_03385"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012888139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HPC11126.1"
                     /translation="MDSSSETPAPGGHSLLEMSGIEKSFPGVRALRAASFALRAGEIH
                     ALVGENGAGKSTLIKILTGVHRRDGGEIRLLGQPAEFRRPLEAQRAGIATIYQEFTLV
                     PSLSVAANIFLGHEAARRGLIDHGRERRAAAALLDRLGAALDPSALVSGLTVAQQQIV
                     EVARALAREARILVMDEPTAALAPHEVAHLFAILRDLAGRGMGIVFISHRLDEVAAIA
                     DRITVMRDGATIAVRRAGEFSRAELIELMVGRPLDQEYPKAPAPIGPVHFEVRGLSGG
                     FIEDVSFAVRRGEVLGLAGLMGAGRTEVARLIFGADRKRRGEIRLGGRPLEIASPRAA
                     IGAGVCLLTEDRRRQGLVLGASARDNFALPNLGSWSRLAWIDRRREQERFAERTAGLN
                     IRLSHPEQRAEELSGGNQQKLLVARWLETNSEVVIFDEPTRGIDVGAKYEMYVLIGRL
                     TALGKCVIVISSELPELLGICDRILVLRRGRVAGEVAEAARATQEDLMALAV"
     gene            2815..>3249
                     /locus_tag="PK112_03390"
     CDS             2815..>3249
                     /locus_tag="PK112_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11127.1"
                     /translation="MENSRGKKHRREAGAARLRQAFSDYGMALVLAALCALFSLLTIS
                     EQHPTGAAAGDNLCAQLAAGTAPGGAVVVIGQATGDDSAMVRTLVGRLANAGRTVRPP
                     VFGSPAEVRARLEALDSAGQAAPLIVTSHAYYAAVTMVAAQVI"
BASE COUNT          504 a         1073 c         1186 g          486 t
ORIGIN      
        1 ggggttcgaa tccctgcacc gctatttctc ttgccggtcg gaaaatgcag ggggcgagcg
       61 cggcgccttc ccagtacctc gccggtctcg gagcacagcc caagcttgcc gaccgtcgcc
      121 cgaccgcgta tacttcacct cgaaaaagaa cccgttgtcg actatccccg gaccggaccg
      181 ccggtgcggg cttaaggacc agcatgccag gacacatcgc cagaacaacc gggcggattg
      241 ccgccctgcg aacagccggg tggatcgccg ccgccagatc aactggccgg atcgctgccg
      301 tcagaacagc cgggcgcagc accgctgcca gaacagtcgg gtggatcgcc gccgccgcag
      361 cggccgcggt gctcggatgc agcggcgccg acgacaccgc caaggtcaaa cagaaccccg
      421 catcggaggg cgccgtgacc atcggcgtgt cgctgctgac gcggacccat ccgttctatc
      481 aggagttgga ggagggtctg aaagaagcgg ccggggcggc cggtttccag gtgcggatta
      541 cggccgggga gttcgatgtc gcccgccaga aagaccaact tcaggatttc ctcactcaga
      601 gagtcgatgc gatcatcgtg tgtccctgcg actctcgttc gatcggcacc gcgatcaagg
      661 cggccaacga ggccggcatt ccggtgttca cggccgatat cgcctgtctg gccgagggcg
      721 tggcgatcgt gacgcacgtg gcctcggaca atgtcgaggg cgggcggctg gccgcccgcg
      781 cgctcgccga ggccatccac ggcgcgggcg cggtcgcgat catcgaccac cccgaagtcg
      841 aatcggtgat ccagcgggtc aaggggttcg aggaggagat cgccaagtac ccggacatca
      901 cgatagtggc gaagctgtcg gggcacggcg tcaaagacca ggccttccgc acggccgagg
      961 acattctgca ggcccatccg gacctggcgg ggtttttcgg catcaacgac gattcggcgc
     1021 tcggcgcgct ggcggcgatc gagaaagccg gcaagctcgg ccgcgtgaaa atcgtcgggt
     1081 tcgacgccgt ccctgaggcg cgccaggcga tcgaggcggg aaagatccac gccgacgtga
     1141 tccagcagcc gcgcaagatc gggcagcgca cggtcgaggc ggtgcaaacc tacctctccg
     1201 gcggcgccgt cgagccgcag atattgatac cgtgcgacct gtttgccaag cccgcggcgc
     1261 agtgaccgga cggccgcggc cgagatatgg attcctcctc tgagacgccc gcccccggcg
     1321 ggcacagcct gctggagatg tcggggatcg agaaatcgtt ccccggcgtt cgggctttgc
     1381 gggcggcgag tttcgccctg cgcgcggggg agatccacgc gctggtcggg gagaacggcg
     1441 ccggcaagag cacgctcatc aagatcctca ccggggtgca ccggcgggac ggcggggaga
     1501 tccggctgct cgggcagccg gcggagtttc ggcggccgct cgaggcgcag cgggcgggca
     1561 tcgccaccat ttatcaggaa ttcactctcg tgccatcgct ctcggtggcg gcgaacattt
     1621 ttctcggcca cgaagcggcc cgccgcggtc tgatcgacca cggccgcgag cggcgcgcgg
     1681 ccgccgccct tctcgaccgc ctgggcgcag ccctggatcc ctcggcgctc gtctccggcc
     1741 tcacggtggc ccagcagcag attgtcgaag tggcgcgggc gctggcccgc gaggctcgca
     1801 tcctcgtgat ggacgagccg accgccgccc tcgcccctca cgaggtcgcg cacctcttcg
     1861 ccatccttcg cgacctggcc gggcggggga tggggattgt cttcatcagc caccgcctcg
     1921 acgaggtggc ggcgatcgcc gaccgcatca cggtcatgcg cgacggggcg acgatcgcgg
     1981 tccgtcgggc aggggagttc tcgcgggcgg agctgatcga gctcatggtg gggcggccgc
     2041 tcgaccagga gtacccgaag gcgccggccc cgatcgggcc cgtacatttc gaggtgcgcg
     2101 gactgagcgg cgggtttatc gaggatgtct cgttcgccgt tcggcggggg gaggtgctcg
     2161 ggctggcggg actgatgggt gcgggccgga ccgaggtcgc ccggctcatt ttcggggccg
     2221 accggaaacg gcgcggcgag atccggctgg gcggccggcc gctggaaatc gcctcgccgc
     2281 gcgcggcgat cggcgcggga gtatgtctac tcaccgagga ccggcgccgc caggggctcg
     2341 tgctgggggc ctcggcccgg gacaatttcg cgctgcccaa tctcggttcc tggtcgcgcc
     2401 tggcctggat cgaccggcgc cgcgaacagg agcggttcgc cgagcggacg gccggtctca
     2461 acatccggct ttcccacccg gagcagcggg cggaggagct ctcgggcggc aaccagcaaa
     2521 agctgctcgt ggcccgctgg ctggagacca attcggaagt ggtcattttc gacgagccga
     2581 cacgggggat cgacgtcggc gccaaatacg aaatgtacgt cctgatcggc cggctcacgg
     2641 cgctcggcaa atgcgtcatc gtgatctcct cggagcttcc ggaactcctc gggatctgcg
     2701 accgcattct cgtgctgcgg cgggggcggg tggccggcga ggtggccgag gcggcccgcg
     2761 ccacgcagga ggacctgatg gcgctggccg tgtgaggccg cagaggaaag gcgcgtggaa
     2821 aattcacggg gcaaaaaaca ccgccgcgag gcgggcgcgg cgcggctgcg gcaggcgttc
     2881 tccgactacg gcatggcgct cgtgctggcc gccctgtgcg cgctgttttc gctgttgacg
     2941 atttccgagc agcacccgac cggcgcggcg gccggcgaca acctgtgcgc ccagctcgcc
     3001 gccgggacgg cgccgggggg tgcggtggtg gtgatcggcc aggcgaccgg cgacgattcg
     3061 gccatggtgc ggacgctagt cgggcgactg gcgaacgccg gccggacagt ccggccgccg
     3121 gtgttcggtt cgcccgcgga ggtgcgggcg cgactcgagg cgctcgattc ggccggccag
     3181 gcggccccgc tgattgtgac cagccacgcc tactatgccg ccgtgacgat ggtggcggcg
     3241 caggtgatc
//
LOCUS       DAOOKS010000154         3234 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_36152_length_3234_cov_3.815980, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000154 DAOOKS010000000
VERSION     DAOOKS010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3234
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36152_length_3234_cov_3.815980"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..1328
                     /locus_tag="PK112_03395"
     CDS             114..1328
                     /locus_tag="PK112_03395"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPC11128.1"
                     /translation="MQSRYKYRAADAAGRTREGVLLADSREQVLEHLAGQQLVPIRVA
                     AVAGRRGFSLLSFFSGPHYERLIAFTANLGTMYRAGVPLLRALALIRIGPAAGHFNRA
                     IDDIRLAVQAGRPLSQAMAAHDDLFARAYVAGVAAGEESGKLDEILAQLRTMLEQDLE
                     LSRAVKAGTRYPLMVVGALGAAALVLVLYVVPRFTAFYGQFNAQLPLPTRLLIGLQHT
                     VSAYWPALLMALVLAALAVRQAIGTERGKSWLDHRLLRLPIVGPLIIKSNVARFAAMF
                     HVLFQAGLPIIACLEILAETVKNSALAGEIRTMAGLFREGRDSRLAEHEFRYFPPLAL
                     QMMTIGLESGSLAEVLREVGEHYGKEVQYTSRQLTAVLEPILTFVMGIFVLILALAIF
                     LPMWNLIRVFNG"
     gene            1415..1840
                     /locus_tag="PK112_03400"
     CDS             1415..1840
                     /locus_tag="PK112_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPC11129.1"
                     /translation="MNQLRNRRGFTLIELVIIIVILGILAAVAIPKYQDLSGEAREAS
                     ARSALGSMRSAVTIFYANQAVQTGTATWPSLADLQTRGTVMAQGLPPNPYQSNAPDSV
                     VDGTGQVRGTVIGNRGGWVYDPVSGELWANTDTHDENEW"
     gene            1830..2318
                     /locus_tag="PK112_03405"
     CDS             1830..2318
                     /locus_tag="PK112_03405"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPC11130.1"
                     /translation="MSGERIRPDPARSDRRLSVHPAGFTLVELVIIIVILGILAAVAI
                     PQLADMSGSAKTAATRKEMGELRRALVGSPEIVAGGRPVAAGFQGDVGRLPGRLSDLT
                     VRPDSVPPYDRLARYGWNGPYIDPSGGNYLSDAWGVPYVYDPDARILLSVGGGDTLRV
                     TL"
     gene            2326..>3234
                     /locus_tag="PK112_03410"
     CDS             2326..>3234
                     /locus_tag="PK112_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11131.1"
                     /translation="MRSLLKKFTAGAHAPSSPASTGPAERPLAPRGRFLIGRTLAGRV
                     SDDALSLAAVRHFGARRIVTDVLHADLPPGALPEAGSEFAISQVADFWSRHGGRGARF
                     AACVAGDGVTVRTFCLPRMSRADLASAVEIEASRQIPFPVGEFTCGFRPVARLEGEGG
                     GRCRVALLAATHRLLRQALVPLEQAAVPVQAVYAGAQAVGVLLTALPNFDPDRIYTLV
                     RLGLRESETAFYRAGNLEFSHHGGTGTAMLGTAGDEARREFFAETLASEIQTSLDYFA
                     GQHLRLHEPEVFVYGGAAGHTRLLRLL"
BASE COUNT          472 a         1143 c         1055 g          564 t
ORIGIN      
        1 cggtctggag gccgtgcggg ctggccgcgt ctgccttgag gaactgctca aagagacctc
       61 gaacgccgag gattacctcc ggccggggga gagcgcgtcg cggagagccg tccatgcaga
      121 gccggtataa gtatcgcgcg gccgacgcgg cggggcggac ccgcgaggga gtgctcctgg
      181 ctgacagccg cgagcaggtg ctggagcatc tggccgggca gcagctggtg ccgatccgcg
      241 tggccgcagt cgccggccgg cgtggatttt cgctcctcag cttcttctcc gggccgcact
      301 acgagcgcct gattgcgttt acggccaatc tgggcacgat gtaccgggcg ggcgtgccgc
      361 tgctccgggc cctggccctg atccgcatcg ggcctgccgc cgggcacttc aaccgcgcga
      421 tcgacgacat ccgcctggcc gtccaggccg gccgcccgct ctcacaggcg atggcggccc
      481 acgacgacct cttcgcgcgc gcctacgtcg ccggcgtcgc cgccggcgag gagtccggca
      541 agctcgacga aatcctcgcc caattgcgca cgatgctcga acaggacctc gagctctcgc
      601 gcgcggtcaa ggcgggcacc cggtatccgc tgatggtggt gggggcgctg ggtgccgccg
      661 ccctcgtgct cgtgctgtac gtggtccccc ggttcaccgc tttctatggg cagttcaacg
      721 cgcagctgcc gctgccgacg cgcttgctga tcgggctcca gcatacggtc tccgcctact
      781 ggccggccct gctgatggcg ctcgtgctcg ccgcccttgc agtgcgccag gcaatcggaa
      841 ccgaacgggg caagtcgtgg ctcgaccacc gccttctgcg tctgccgatc gtcggtccgc
      901 tgatcatcaa gagcaacgtc gcccggttcg ccgcgatgtt ccatgtgctc tttcaggccg
      961 gcctccccat catcgcctgc ctcgaaattc tggccgaaac ggtcaagaac agcgcgctgg
     1021 ccggagaaat ccgcaccatg gcgggtctct tccgcgaggg ccgcgacagc cgtctggccg
     1081 agcacgagtt ccggtacttc ccgcccctgg cgcttcagat gatgaccatc gggctggagt
     1141 ccggctccct cgccgaggtt ctccgcgagg tcggcgagca ctacggcaag gaggtgcagt
     1201 acacctcccg ccagctcacc gctgtgctcg agcctattct cacgtttgtc atgggcattt
     1261 tcgtgctcat tctggcgctg gcgatcttcc tgccaatgtg gaacctgatc cgggtcttca
     1321 acggctgagg cgggctaaaa agggcgggat attattccga taacgataga agagtaagtg
     1381 tcaaataatc agccccctgc aggaggtaga ttccatgaac caactccgca accggcgcgg
     1441 cttcacgctg atcgagctcg tcatcatcat cgtgatcctc ggcatcctcg ccgccgtcgc
     1501 catcccgaaa taccaggatc tttcgggcga agcccgcgag gcgtcggccc gcagtgccct
     1561 cggctcgatg cggtccgcgg tcaccatctt ctacgccaac caggccgtcc agaccggcac
     1621 cgccacctgg ccctcgctgg ccgatctgca gacccgcggc acggtcatgg cgcagggcct
     1681 gccgccgaac ccgtatcagt ccaatgcacc cgactcggtc gtcgacggca ccggccaggt
     1741 gcgcggcact gtcatcggca accgcggcgg gtgggtttat gacccggtct ccggcgagct
     1801 ctgggccaac accgacaccc acgacgaaaa tgagtggtga gcgcatccgg cccgaccccg
     1861 cccgctccga tcgacggctc agtgttcatc cagccggctt cacgctggtc gaactggtca
     1921 taatcatcgt catcctcggg attctcgccg ccgtggccat cccgcagctc gccgacatga
     1981 gcgggagcgc taaaacggcg gccacccgaa aggaaatggg cgagctgcgg cgggcgctgg
     2041 tcggctcgcc tgagattgtc gccggcggcc ggccggtggc cgctggcttc cagggagatg
     2101 tcggccggtt gcccggacgg ctgagtgatc taacggtccg gcccgactcg gtcccgccgt
     2161 acgatcggct cgcccggtac ggctggaacg gcccgtacat cgacccctcc ggcggaaatt
     2221 acctcagcga tgcctggggc gtaccgtatg tgtacgaccc ggacgcccgg atcctcctct
     2281 ccgtcggggg cggagacact cttcgcgtga cgttgtgaca ccgctatgcg ctcgctactg
     2341 aagaagttca ccgccggcgc acacgcgccc tcctccccgg cctctaccgg cccggcggaa
     2401 cgcccccttg cgccccgcgg ccgctttctg atcgggcgta cgctcgccgg gcgggtcagc
     2461 gatgatgccc tgtccctggc ggctgtccgc cacttcggtg cccggcggat tgttaccgac
     2521 gtccttcatg cggatctccc gccgggcgca cttcccgagg ccggttccga gttcgcaatc
     2581 tcgcaggtcg ccgacttctg gagccggcac ggcgggcgcg gcgcccgctt cgcagcctgc
     2641 gtcgccgggg acggcgtcac ggtccggacg ttctgcctgc cccgaatgag tcgggccgat
     2701 ctggcctcag ccgtcgagat tgaggccagc cgccagatcc ctttcccggt cggcgagttc
     2761 acctgcggtt ttcgcccggt ggcgcgactc gagggcgagg gcgggggccg gtgcagggtg
     2821 gcgctgcttg cggccacgca ccggctcctc cgccaggcac tcgttcccct ggaacaggcc
     2881 gcggttccgg ttcaggccgt gtatgccgga gcgcaagcgg tcggcgtgct cctgaccgcg
     2941 ctgccgaatt ttgaccccga ccgcatctac acgctggtgc gtctcggact gcgcgagagt
     3001 gaaacagcct tctaccgggc cggcaatctc gaattctccc accacggcgg caccggcacg
     3061 gcgatgctgg ggacggcggg cgacgaggcg cggcgggagt tcttcgccga aacgctggcc
     3121 tcggagatcc agacgtcgct cgattatttc gcgggccagc acctgcgcct ccacgagccg
     3181 gaggtgtttg tttacggcgg cgcggccggc cacacccgcc tgctgaggct gttg
//
LOCUS       DAOOKS010000155         3224 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_36304_length_3224_cov_3.710634, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000155 DAOOKS010000000
VERSION     DAOOKS010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36304_length_3224_cov_3.710634"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1274)
                     /locus_tag="PK112_03415"
     CDS             complement(<1..1274)
                     /locus_tag="PK112_03415"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPC11132.1"
                     /translation="MTDIPCPLGEAARRRPAAPFLVTPAREYSYAEANRAVNRRAAAL
                     ARLETRLGPLRRLALLVPKAGDEPSLDVIITFLAAGRAGATALVVDGALPAPLVRAAL
                     PAEQGIVAVVAQDRREHIPFAAATWEEVAEIEAQILLSPAEADRTDFRRNLRLEATGV
                     LTSGSTGTPKLCLHTVGNHYYSALGSNENLPLAEGDRWLLSLPLHHVGGIAILYRAML
                     AGAAVVPGRRPANPAESIETLGVTHCSMVPTQLRRYLAEGGHRAGLKAVLIGGAAAPR
                     GLIEAAAARGLPLFTSYGLTEMCSQVTTTAPDDPLRRLFGSGRLLPFRELRIDESGEI
                     LVRGPVLSPGYLESNGLRTIADAEGWFHTGDRGELEPDGYLRVTGRKDHMLISGGENV
                     QPELEEKMLLELPDVAEAVVVAVEDAQYGQRP"
     gene            complement(1267..2382)
                     /gene="menC"
                     /locus_tag="PK112_03420"
     CDS             complement(1267..2382)
                     /gene="menC"
                     /locus_tag="PK112_03420"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: protein motif:HMM:TIGR01927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HPC11133.1"
                     /translation="MRIAQVQLYRYVLPFRRPPVIRGEELRSREGMLVKVTLEDGATA
                     WGEAAPLPGHSRESLADAERELRELRAGVQGAQIPAGLERLDGGFGNWLGHSWLTASV
                     SVAMETAVLQLVAHRQGKSLHRLLCPNPRERITVNALLSGSADEAFGQAERRKGEGFG
                     AFKLKVGRRPFDEDIEAVRRVRETIGPRAKLRLDANRAFDIPTAIRFGQAAAPYDIEY
                     IEEPVRSFLELLELTHRGGFRLPVALDESLRTISPKALSQVRGVRAIVLKPTLLGFEW
                     AMWFARRARRAGMLAVISSAYESSLTLAMLAEIGACLNDGEAGENVAMGLDTQDALAE
                     DLTVEPLRIAQGQIDLSALPAGTFEVRPDRLGEPAHD"
     gene            complement(2379..>3224)
                     /locus_tag="PK112_03425"
     CDS             complement(2379..>3224)
                     /locus_tag="PK112_03425"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HPC11134.1"
                     /translation="RPKTLFASAAPVVMGTAMAAEAGAFHLWSALAALAGALLIQIGT
                     NFANDYSDFKSGADAIARVGPIRVTQAGLVTPGAMKAATALVFGLAVLCGAYLVYRGG
                     WPVTVIGLSSVLWGILYTAGPYPLGYIGLADLFVFVYFGPVAVGGTYYVQTLAITPPV
                     LVAGAGTGLFSVAILTVNNLRDIESDRRAGKRTLAARFGRTFAQWEYVLAVAIAAGIP
                     AALALLTEGHSSAIIASLTILPALAAFRTIRRATDGPTLNRLLATTGKLLLLYSLLFS
                     IGWWW"
BASE COUNT          533 a         1175 c         1054 g          462 t
ORIGIN      
        1 aacggccgct gcccgtactg cgcgtcctcg accgcgacca ccaccgcctc ggcgacatcc
       61 ggcaactcca gaagcatctt ttcctccagc tccggctgca cgttctcgcc gccggagatg
      121 agcatgtggt cctttcggcc ggtcacgcga agatacccgt cgggctccag ctccccccgg
      181 tcgcccgtgt ggaaccatcc ctcggcgtcg gcgatcgtcc gcaggccatt cgattccagg
      241 taccctggcg agagcacagg cccccgcacg aggatctcgc ccgactcatc gattcgaagt
      301 tcccggaagg gcaggagtct gcccgacccg aacagccgcc ggaggggatc gtccggggcc
      361 gtcgtggtca cctgagagca catttccgtc agaccgtagg aggtgaacag cggcagaccc
      421 cgcgcggcgg ccgcttcgat caggccgcgc ggcgccgccg ctcccccgat caggacagcc
      481 ttcaacccgg cccggtggcc tccctcggcg aggtaccgcc gcagttgcgt cggcaccatc
      541 gagcagtggg tgacgccgag cgtctcgatc gactccgccg gattcgccgg tcggcgcccc
      601 ggcacgaccg ccgcgccggc gagcatcgcc cggtacagga tcgcgatccc gcccacgtgg
      661 tgcagcggca gcgacaacag ccaccggtcg ccctccgcca gcgggaggtt ctcgtttgat
      721 ccgagcgcgc tgtagtagtg gttcccgacc gtgtgcagac acaacttcgg cgtcccggtg
      781 gaccccgagg tgagcacgcc ggtcgcctcc aaacggaggt tgcggcggaa atccgtccgg
      841 tccgcttccg ccggcgagag gagaatctgc gcctcaattt cggcaacctc ctcccaggtg
      901 gcggcggcga aggggatatg ctcccggcgg tcctgagcga cgaccgcgac aattccctgc
      961 tccgccggca gcgccgcccg caccagcggc gccggcagag cgccgtcgac caccagcgcg
     1021 gtcgccccgg ctcttcccgc ggccaggaac gtgatgatga cgtcaaggga gggctcgtcc
     1081 ccggctttcg ggacaagcag agcgagccgc cgcagcggtc cgagccgcgt ttccaagcgg
     1141 gccagcgccg ccgcgcggcg attcacggcg cggttggcct cggcgtagct gtactcccgc
     1201 gccggcgtca cgagaaaggg cgccgcgggc cgccggcgcg ccgcctcccc cagcggacag
     1261 gggatatcag tcatgcgccg gttctccgag gcggtccgga cgcacttcga aagtccccgc
     1321 gggaagggcg gacaggtcga tttgcccctg cgcgatgcgg agcggttcca cggtcagatc
     1381 ctcggcgagc gcgtcctgcg tgtccagtcc catggcgaca ttctcgccgg cctcgccgtc
     1441 gttgaggcag gcgccgattt cggccagcat cgccagtgtg aggctcgatt cgtaggccga
     1501 gctgatcacc gccagcatcc ccgcccggcg cgcccgccgg gcaaaccaca tcgcccactc
     1561 gaatcccagc agggtcggct tgagcacgat cgcccgcacc ccgcggactt gcgacagcgc
     1621 tttcggcgag atcgtgcgca ggctctcgtc gagcgcgaca ggaaggcgga atccgccgcg
     1681 gtgagtcagt tcgagcagct cgagaaaact gcgcaccggc tcctcgatgt actcgatgtc
     1741 gtacggcgcg gccgcctgcc cgaaccggat cgcggtgggg atatcgaacg cccggttggc
     1801 atcgagccgg agcttggcgc gcgggccgat cgtctcgcgc acccggcgca ccgcctctat
     1861 gtcctcgtcg aacggccgcc gccccacttt gagcttgaac gccccgaaac cttcgccctt
     1921 gcggcgctct gcctgcccga acgcctcatc cgccgatccg ctcagaaggg cgttgacggt
     1981 gatcctctcc cggggattgg gacagagcag gcggtgcagc gatttcccct ggcggtgtgc
     2041 caccagttgg agcacggcgg tctccatcgc gaccgacacc gaggctgtca accagctgtg
     2101 gcccagccag ttgccgaaac cgccatcgag ccgctcgaga ccggcgggga tttgcgcccc
     2161 ctgcacaccg gcccggagtt cccgcagttc ccgctcggca tcggccagcg attcccggct
     2221 gtgtcccgga agcggcgccg cctcccccca ggccgtggcg ccgtcctcga gcgtcacttt
     2281 cacaagcatg ccctcgcggg agcgcagttc ctcgccccga atgaccggcg gccgccggaa
     2341 cggcagtaca tagcggtaga gctgaacctg cgcgattctc accaccacca tcctatcgaa
     2401 aacagcaggc tgtacagcaa gagcagtttc cccgtcgtgg ccagcagcct gttgagcgtc
     2461 ggtccgtcgg tggcgcggcg gatcgtacgg aacgccgcca gcgccggaag tatcgtgagc
     2521 gatgcaataa tcgccgacga gtgcccctcg gtcaacagcg cgagcgcagc gggaatgccc
     2581 gcggcgattg cgacggcgag gacatactcc cactgggcga aggttcgccc gaaccgcgcg
     2641 gcgagcgtgc gcttgccggc ccgacggtcg ctctcgatgt cgcggagatt gttgaccgtc
     2701 aggatcgcca cggagaacaa cccggtcccc gccccggcga ccagcaccgg gggagtgatg
     2761 gccagcgtct gcacgtaata ggtcccgccg accgccacgg gtccgaagta gacgaacaca
     2821 aagaggtcgg ccagaccgat atagccgagc gggtacgggc cggccgtgta gagaatcccc
     2881 cagaggacgg agctgagtcc gatgacggtc accggccagc caccgcgata tacgaggtag
     2941 gcgccgcaga gcacggccag cccgaacacc agcgccgtgg ccgccttcat cgcgcccggt
     3001 gtgaccagac ccgcctgagt cacccggatg ggaccgaccc gggcgatcgc atcggccccc
     3061 gatttgaaat cggaataatc gttggcgaaa ttggtcccga tctggatgag cagcgccccg
     3121 gccagcgccg cgagcgccga ccagaggtga aaggcgcccg cctccgccgc catcgccgta
     3181 cccatcacca ccggcgccgc ggaggcaaag agcgtcttgg gccg
//
LOCUS       DAOOKS010000156         3223 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_36322_length_3223_cov_3.121212, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000156 DAOOKS010000000
VERSION     DAOOKS010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36322_length_3223_cov_3.121212"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="PK112_03430"
     CDS             complement(<1..495)
                     /locus_tag="PK112_03430"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HPC11135.1"
                     /translation="MNSVMKWAFAAALAMSCLASGATPGRSETVAVLMSDTLTSTTRT
                     LQGAKKTIEAANPGIELRTFMIGQSPAGDQAVADSVRSCNPRLVLTIGSTATGIAQHH
                     LPQVPIVFAGVMYPVLSGFVQTIQEPGGHITGASLDIPNEVQFRYFKKIIPGIERIGV
                     LYTAN"
     gene            812..>3223
                     /locus_tag="PK112_03435"
     CDS             812..>3223
                     /locus_tag="PK112_03435"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPC11136.1"
                     /translation="MRNRLHMIFCVVGCLAVLALPSAAEAGVTGKVSGVVLDSATGEP
                     IVGAIIRVVGTDMGALTDVDGEYFIINLPGGSHDVSVSYMGYEPVTKTGVRVLIDLTT
                     PLDFRMVPAPVELGRQVVVSAANPIVQQDLTATRIIFTADRLEELPNIVSVQSVLQKY
                     PGVVEDREQALHVRGGRAGQVTYFYDGFNIQDPFTATQGIEIIPSALEELSLTSGGYT
                     AEYGEALSGVVSAVSRDGTAQYHGRLKMYEGFTHPYDVDKGDWGGLERIGNRSLAFDL
                     SGPLPGLDPRRYTFFSAGQYLTDPTSLPHNEVTSWTGTGKLTMYPASGWKFKTNVTYY
                     SADGSVYDHRDVNNRSYDFNLDGLPVFEKEAYLVGFSTDYAFNERAVLTGAVNRFHTR
                     TKSAPDHLFDTYWDEWPGYAVDSAGVYNGTIDDENYLNEPDYSDPMQVVGFTVGDDYY
                     PTYRLRETWYDAFSLRFMHQVNKWNQLKTGAEFRQYDINWDFKQFFNDRPYGEKYQSK
                     PTYASFFVQDKMEYDYFIVNLGLRYDFHNADISYNADPYAATPVWKKAETKHGLSPRL
                     GVSFPIAENSKMHFNYGVYYQEPRFQYLYMNYQGDITTGLPLLGNPDLEPERTTSYEL
                     GLDHLLDNRWHLNVTAYYKDIEDLVTARQDGLIAGREVTRITNGDYGSVKGIDLALEV
                     LPNGGCFTGSVSYGYMIATGNGSYALEPYYTYITSTTDTVQPFTEYPLDFDQRHTVTG
                     VLSYRVPSDWDGRLLGQKLPGDWGLTLVGYYGSGLPYTKTDQSGNRVGERNAWRLPAN
                     YKVDLR"
BASE COUNT          610 a         1103 c          963 g          547 t
ORIGIN      
        1 gttcgccgtg tagaggacgc cgatgcgttc gatccccggg atgatcttct tgaagtagcg
       61 gaactgaact tcgttgggaa tatccagcga cgccccggtg atgtgcccgc cgggttcctg
      121 aatcgtctgc acaaacccgg agaggaccgg gtacatgacg ccggcaaaga caatcgggac
      181 ctgcggcagg tggtgctggg cgatccctgt ggccgtgctc ccgatggtca gcaccagccg
      241 gggattgcag ctccggaccg aatcggcgac cgcctggtcg ccggccggcg actggccgat
      301 catgaacgtg cgcagctcaa tccccggatt ggccgcttca atcgtcttct tggccccctg
      361 cagagtcctc gtggtcgacg tgagcgtgtc gctcatcagc acggcgacag tttcggatct
      421 ccccggcgtt gctcctgagg cgaggcagct catggcgagg gctgccgcga acgcccattt
      481 catcacgctg ttcatcgggt gagcacgctt atcctctctg tttcctcgac gttgtagccg
      541 tcaccgttgc ttcccgtcga atccgggcgt gacgccgtca tgacacgtct cagatatcgg
      601 cgcggccccc cggaaacttg atccggacgg cggaaagcgc cggcaaaaaa aggactcgat
      661 gatccccgac cgggtgtcaa ggatttacag gggccgccga tactctcgaa aagaccggca
      721 agctgcgttc cgcgcccggc gcggaccatc gacataaatc gaggaagcaa acaactttcg
      781 attgtggaaa catatgcgag gaggatttgc cttgcgtaat cggctgcaca tgatcttctg
      841 cgtggtcggc tgcctcgccg ttctcgcact gccctccgcc gccgaggccg gggtcaccgg
      901 caaggtgtcg ggggtggtgc tcgacagcgc gacgggcgag ccgatcgtcg gcgccatcat
      961 ccgcgtcgtc ggcaccgaca tgggcgccct gaccgacgtg gacggcgagt acttcattat
     1021 caacctcccg ggcggctccc acgacgtctc cgtgtcctac atggggtacg aaccggtgac
     1081 caaaacgggc gtgcgggtgc tcatcgacct gaccaccccg ctggacttcc gcatggtgcc
     1141 cgcgcccgtc gaactcggcc ggcaggtggt ggtctcggcc gccaacccca tcgtccagca
     1201 ggacctgacc gccacccgca tcatcttcac ggccgaccgg ctggaggaac tccccaacat
     1261 cgtgagcgtc cagtcggtgc tgcagaagta cccgggcgtg gtcgaagacc gggagcaggc
     1321 gctccacgtc cgcggcggcc gcgccggcca ggtgacgtac ttctatgacg gattcaacat
     1381 tcaggatccg ttcaccgcga cccagggaat cgagatcatc ccctcggccc tggaagagct
     1441 gtcgctgacc tcgggcggct acacggccga gtacggcgag gccctctccg gcgtcgtgag
     1501 cgcggtctcc cgcgacggca ccgcgcagta ccacggccgc cttaagatgt acgaaggatt
     1561 cacccacccc tacgacgtgg acaaggggga ctggggcggc ctcgaacgga tcggcaaccg
     1621 ctccctggcc ttcgacctct ccgggccgct cccggggctc gacccccggc gctatacctt
     1681 cttcagcgcc ggccagtacc tgaccgaccc gacctccctg ccgcacaacg aggtcacctc
     1741 gtggaccggg accgggaagc tcaccatgta tcccgccagc gggtggaagt tcaaaaccaa
     1801 cgtcacatac tactcggccg acggttcggt gtacgaccac cgcgacgtca acaaccggtc
     1861 gtatgatttc aatctcgatg gtctccccgt gttcgagaag gaggcctacc tggtggggtt
     1921 cagcaccgac tacgcgttca acgagcgggc ggtcctcacc ggcgcggtca accgtttcca
     1981 cacccggacg aaatcggccc cggatcacct cttcgatacc tactgggacg aatggccggg
     2041 ctacgcggtc gactccgccg gcgtgtacaa cggcaccatc gacgacgaga actacctgaa
     2101 cgaacccgac tactccgatc ccatgcaggt ggtcggtttc accgtcggcg acgactacta
     2161 cccgacctac cggctgcgcg agacgtggta cgacgccttc tcgctgcgtt tcatgcacca
     2221 ggtgaacaag tggaaccagt tgaagacggg cgccgagttc cggcagtacg acatcaactg
     2281 ggacttcaag cagttcttca acgaccggcc ctatggcgag aagtaccaga gcaagccgac
     2341 ctatgcgtcg ttcttcgtgc aggataagat ggagtacgac tacttcatcg tcaacctcgg
     2401 cctgcgctac gatttccaca acgccgacat cagctacaac gccgatccgt acgcggcgac
     2461 gccggtgtgg aagaaggccg agaccaagca cgggctgtcc ccccgcctcg gggtctcgtt
     2521 ccccatcgcg gagaacagca agatgcactt caactacggc gtgtactacc aggagccccg
     2581 cttccagtac ctctacatga actaccaggg cgacatcacc accggtctgc cgctgctggg
     2641 gaatcccgac ctcgaacccg agcggaccac ctcgtatgag ctcggcctcg accacctcct
     2701 cgacaaccgc tggcacctga atgtcaccgc ctactacaag gacatcgagg atctggtgac
     2761 ggcgcggcag gacggcctga tcgccgggcg ggaagtgacc cgcatcacca acggcgacta
     2821 cggctcggtc aaggggatcg acctcgccct ggaagtgctg cccaacggcg gctgcttcac
     2881 cgggtcggtc tcctacgggt acatgatcgc gaccggcaat ggctcgtacg cgctcgaacc
     2941 ctactacacg tacatcacct cgaccaccga caccgtgcag ccgttcaccg agtaccccct
     3001 cgacttcgac cagcgccaca cggtcaccgg cgtcctgagc taccgggtgc cgtccgactg
     3061 ggacggccgg ctgctgggtc agaagctgcc gggcgactgg gggctgaccc tggtcgggta
     3121 ctatggctcc ggcctgccgt acaccaagac cgaccagagc ggcaaccggg tgggcgagcg
     3181 caacgcctgg cgcctccccg ccaactacaa ggtcgacctc cgc
//
LOCUS       DAOOKS010000157         3205 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_36587_length_3205_cov_4.251111, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000157 DAOOKS010000000
VERSION     DAOOKS010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3205
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36587_length_3205_cov_4.251111"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2135)
                     /gene="dnaE"
                     /locus_tag="PK112_03440"
     CDS             complement(<1..2135)
                     /gene="dnaE"
                     /locus_tag="PK112_03440"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HPC11137.1"
                     /translation="MFANFVHLHTHSQYSLLDGACRLDDVIALAREQRMPALAITDHG
                     NMFGAIEFYTKARRAGLKPIIGCEAYVAGGSRLDKKPSSRWPDGGFHLVLLVRNLTGY
                     QNLVKLASAAFLEGFYHRPRIDKELLRRHSEGLIATSACLKGEVAWHLLQGRTDEAVA
                     AARELQDIFGEGNFFLEIQNHGLEQELQNIPRMEAISRETGIPLVATNDCHYLRREDA
                     EAHDALLCIQTGKMVEDTQRMRYRTDQLYFKSAEEMEASFGDFKEALRNTIRIAEACN
                     LELEMDRLLLPVFPIPAAFSNPDDCLEHLCREGCRERYGRLSDEIEGRLRYELGVIRQ
                     MGYAGYFLIVKDFCDYARREGIPVGPGRGSAAGSLVSYALRITNIDPLAFGLLFERFL
                     NPERVSMPDIDIDFADRGRDKIIKYVIDKYGEDNVCQIITFGTLAARGVIRDIGRVLG
                     MPYGEVDRIAKLVPEGPGQTIELALKKVPELAELRKKDERVDRILSYAVTLHGLARHC
                     STHAAGVVIAPSALTSYVPLFKSGKDEITTQYDMKMVEKIGLLKMDFLGLRTLTVIDD
                     AVRMIRENDPGAALDLDNLSLDDPEVFRLFARGDTVGIFQFESPGMRDYLRKLNPSKF
                     TDLVVMNALYRPGPLDSGMIDIYIDRKHGRQRIEFVHPSLEEILGDTYGVIVFQEQVL
                     KIANVLAGYPLGRADLLRTYEALFSWREGL"
     gene            complement(2423..3046)
                     /locus_tag="PK112_03445"
     CDS             complement(2423..3046)
                     /locus_tag="PK112_03445"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HPC11138.1"
                     /translation="MKHVVLALALVLLLSPLASALSFSGSLASPAGVTASGYWAQQGF
                     KIEWMIEDQPDGSWFYQYWLTDAQGNPWEVAAVSHFTLEISPDATASDFWGYSGAVEF
                     GDLNGMSSAMKLDFTATHYKFYSNRAPVWGDFYAKDGRAGGLGLNEAYNAGFGAPDPT
                     AMPGNGSIGNKILRPDTSTTVVPEPGTMLLMGIGLAGAGVVRRWRRT"
BASE COUNT          555 a          913 c         1163 g          574 t
ORIGIN      
        1 aggccctcgc gccagctgaa cagggcctcg taggtgcgca gcaggtcggc gcgcccgagc
       61 gggtagccgg cgaggacgtt ggcgatcttg agcacctgct cctggaaaac gatcacgccg
      121 taggtatcgc cgagaatttc ctcgagcgac gggtggacga attcgatcct ctgccggccg
      181 tgcttgcggt cgatgtagat gtcgatcatg cccgagtcga gcggaccggg gcggtagagg
      241 gcgttcatca cgaccaggtc ggtaaacttc gacgggttga gcttgcggag gtagtcgcgc
      301 ataccggggg actcgaactg gaagatgccg accgtgtcac cgcgggcgaa gaggcggaac
      361 acctcggggt cgtcgagcga gaggttgtcg agatcgaggg ccgcgccggg gtcgttttcc
      421 cgaatcatgc gcacggcgtc gtcgatgacg gtcagggtgc gcaggccgag aaagtccatc
      481 ttcagcaggc cgatcttctc gaccatcttc atgtcgtact gggttgtgat ctcgtccttg
      541 ccggacttga agagggggac gtagctggtg agggcggagg gggcgatgac gaccccggcg
      601 gcatgggtgg agcagtggcg ggccaggccg tggagggtga ccgcgtagga gaggatgcgg
      661 tcgacgcgct cgtctttctt gcgcagctcg gccagctcgg ggactttctt cagcgccagc
      721 tcgatcgtct gcccggggcc ctccgggacg agcttggcga ttctgtcgac ctcgccgtag
      781 ggcatgccga ggacgcggcc gatgtcgcgg atgacgccgc gggcggcgag ggtgccgaaa
      841 gtgatgatct ggcagacgtt gtcctcgccg tacttgtcga tgacgtactt gatgatcttg
      901 tcgcggccgc ggtcggcgaa gtcgatgtcg atgtcgggca tggacacgcg ctcggggttg
      961 aggaagcgtt cgaagagaag gccgaaagcg agcgggtcga tgttggtgat gcggagagcg
     1021 taggagacga gcgacccggc ggccgagccg cggccgggac cgaccgggat cccctcgcgg
     1081 cgggcgtagt cgcagaaatc cttgacgatg agaaagtagc cggcatagcc catctgccgg
     1141 atcaccccca gctcgtagcg gagacgcccc tcgatctcgt cgctcagccg gccgtagcgc
     1201 tcccggcagc cctcccggca gaggtgctcc aggcagtcgt cggggttgga gaaggcggcg
     1261 ggaatcggaa agaccggcag gaggaggcgg tccatctcga gttcgaggtt gcaggcctcg
     1321 gcgatgcgga tggtgttgcg gagggcctcc ttgaaatcgc cgaacgaggc ctccatctcc
     1381 tcggccgact tgaaatagag ctggtcggtg cggtagcgca tgcgctgggt gtcctcgacc
     1441 atctttccgg tctgaatgca caggagcgcg tcgtgcgcct cggcgtcctc gcggcggagg
     1501 tagtggcagt cgttggtggc gacgagcggg attccggttt cgcgtgagat cgcctccatg
     1561 cggggaatgt tctgcagctc ctgctcgaga ccgtggttct ggatttcgag gaagaagttc
     1621 ccttcgccga aaatatcctg cagctcgcgg gcggcggcga ccgcttcatc ggtccgcccc
     1681 tggaggaggt gccaggcgac ctcccctttg aggcaggcgc tggtggcgat cagcccttcg
     1741 ctgtggcgac ggaggagttc cttgtcgatg cgggggcggt ggtagaagcc ttcgaggaag
     1801 gcggccgaag cgagtttgac gaggttctgg tagccggtga ggtttctcac gaggaggacc
     1861 aggtggaaac cgccgtcggg ccagcggctg gaaggtttct tgtcgaggcg gctgccgccg
     1921 gcgacgtagg cttcgcagcc gatgatcggt ttgaggccgg cgcggcgggc cttggtgtag
     1981 aattcgatcg ccccgaacat gttgccgtgg tcggtaatag cgagcgccgg catgcgctgc
     2041 tcgcgggcca gcgcgatcac atcgtcaaga cggcacgccc cgtccagcag cgagtactgg
     2101 ctgtgcgtgt ggagatggac aaaattcgca aacataggct caggccaatc aaccctcccc
     2161 tcttccctgc ttgtacggcg gccggtgtct tcggatcgcg gaaaggtgac ggccgggcgg
     2221 tacgggtcgg gtatgacaag ggcggtcccc gccggggcgg tcgccgccgt ccacagatac
     2281 aaaaaaacag gcgctgcggg gtttcgtcaa acgatttgtg aagggcgggc ggcgggcggg
     2341 cggcgggagg gcgcggggcc ggacgacgaa aggcggtccg gtcggaccgc ctttcggatt
     2401 tggggagatg ttactctcgt gcctaggtgc ggcgccaccg gcgcacgaca cccgcgccgg
     2461 ccaggccgat gcccatgagc agcattgtcc ccggctccgg caccaccgtg gtggaggtgt
     2521 cgggacggag gattttgttg ccgatggagc cgttgccggg catggcggtg ggatcggggg
     2581 cgccgaaccc ggcattgtat gcctcgttca gccccagacc gccggcccgt ccgtctttgg
     2641 cgtagaagtc gccccagacg ggagcgcggt tggagtagaa cttgtagtga gtggccgtga
     2701 agtcaagctt catggcgctg ctcatgccgt tcagatcgcc gaactcaacg gcgccggaat
     2761 agccccagaa atcggacgcc gtagcatcgg gagaaatctc cagcgtgaaa tggctgacag
     2821 ccgcaacctc ccagggattc ccctgggcgt cggtcagcca gtactggtaa aaccaggaac
     2881 catcgggctg gtcttcgatc atccactcaa tcttgaagcc ctgctgcgcc caatagcccg
     2941 aggcagtgac gccggcgggg gacgcaaggg agccggagaa tgagagggcg cttgccagag
     3001 gcgacagcaa caagaccagc gccagtgcaa gcacaacgtg tttcatcggc aactcctcat
     3061 gcagtatagg gttaatgggt gttcggtatt cctgccggcg gtctgtgcgg atcaatgcac
     3121 atctcaccgg ttgtttgccc tgccgtttgg tctgagggtc tccacaacag cgcgattgtc
     3181 tcccagcggg gccgcgacga caagc
//
LOCUS       DAOOKS010000158         3192 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_36814_length_3192_cov_3.432579, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000158 DAOOKS010000000
VERSION     DAOOKS010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36814_length_3192_cov_3.432579"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(568..2547)
                     /locus_tag="PK112_03450"
     CDS             complement(568..2547)
                     /locus_tag="PK112_03450"
                     /inference="COORDINATES: protein motif:HMM:NF025068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HPC11139.1"
                     /translation="MKGRVRARLRRRHALLSALAAGLLLIPGLASAETAPPIGDIHRP
                     FSVRTTDAEARQLQAESAPGLHAALAGAAGPQRLEVPLPTGEAALLELERFDVVAPDA
                     RFVIAGPGGGADLPPSDVLLYRGRIAGDEYSSAFLAVSGSGMINGFIDAAGTRYLLAT
                     PSASLKSGDRTVTIREAAGIGELEVPFCGTEPGSAFDDDLEMVKEAALKTSAGPKLLY
                     LAIDADQAYVNMFGSAVEARDYIVQLAGAASAIYVRDLDLRLWLRFARLWPSGGEPFS
                     PQDLYGFRDYWQVNEDPSPFNIIHMFSGRRDTPYGGVSFISNTCNGNAYGIDALLNGS
                     FTAPVTYPDNGNWDINVFPHEMGHNCGTYHTHDDQYNPHIDDCGNGTPSVSTMMSYCH
                     TFQGYQRNIDLRFHRRVVGIIRSVTYAAGCHGFDCNGNGVPDDADIAAGSSLDVNSDG
                     IRDECQDCNGNGTLDPLEIAGGAPDVDGNGILDACEADCNSNGLPDRYETWALLAADA
                     DGNFRPDACDPDCDNNGQLDYSQIQANLALDLDRNRVLDQCQDCNGNFVPDWMDMGRQ
                     FNVFTGDRVLTGIAEFHEASGVTVAATRLLPRPTTCWPTRPAATCGWRRPTVRCIGTR
                     SAAARSASSSPPAAADSGSPPACSFRRPANCSCST"
     gene            complement(2723..>3192)
                     /locus_tag="PK112_03455"
     CDS             complement(2723..>3192)
                     /locus_tag="PK112_03455"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPC11140.1"
                     /translation="NDFASPPGSGLKQLADRIPHLQSLGVNAVYLGPLFESAAHGYDT
                     VDYWHVDRRLGTDADLAALVRSFHDAGIAVVLDAVLNHTGRDFFAFRDLRLHRLTALV
                     LAPNVGSARLLEKVGFRYEGTRRKDCRLRRRWYDVAVYGLLAREWAGPPARRP"
BASE COUNT          530 a          984 c         1171 g          507 t
ORIGIN      
        1 ggtcgcccgc ctcgcagaca tcgagcacgc ggttgccgtt gacgtcgtgc ggcgaggcgg
       61 gcaggaagtc gagacccgag acctggtcga cagcgctcgt gccgcgcaca aagcggcggt
      121 agtacttacc gagatcgggg aaatactcga acacggcgcc ctcggcgccg atgaagaggt
      181 ggccgttcgg gccgatgcgg agcccccacg gcaagctgag gccgaactca tcggtgaact
      241 cccggacaaa cgcgccggtc gtgccgtcgt attcaagcac ctggttggtg ttgtaggacg
      301 acacgaccag gttgccgttg gggaggaagg ccatgccgcg cgggccggag agaccgccgc
      361 tgccgggcga aacgaacagc ccgagagagg cgccggtcgc ggccaggaag cggtagacgc
      421 cgttgtcgga gctgttgacc agcagggaag cgccgtcggg gctgaatacc atgccgcgcg
      481 gctgcgtcac cccgccgccg ccgggagcga cgaggttgcc gaggaaggct ccggtgctgc
      541 ggttgtagcg gcggacggcg gcgttggtca ggtcgagcac gagcagttcg ccggacggcg
      601 gaatgagcag gccggagggc tgcccgagtc cgccgctgcc ggcggcgatg aagacgctga
      661 gcgtgccgct gccgaccgcg tgccgataca cctgaccgtc ggtcgccgcc acccacatgt
      721 agctgccggc cgggtcggcc agcacgtcgt tggccgtggc aacagacggg tagctgccac
      781 ggtcacgccg ctggcctcgt ggaattcggc gatccccgtg agcacacggt cgcccgtgaa
      841 cacgttgaac tggcggccca tgtccatcca gtcggggacg aagttcccat tgcagtcctg
      901 gcactggtcg agcacgcggt tgcgatcgag atcgagagcc aggttggcct ggatctggct
      961 gtaatcgagt tggccgttgt tgtcgcagtc ggggtcgcag gcgtcgggcc ggaaattccc
     1021 gtcggcatcg gccgccagca gagcccacgt ctcgtaccgg tcgggcaacc cgttgctgtt
     1081 gcagtcggcc tcgcaggcgt cgagaatgcc gttgccgtcg acatcgggag ccccgccggc
     1141 gatctccagc gggtcgagag tcccgttgcc gttgcagtcc tggcactcat cgcgaatacc
     1201 gtcgctgttg acgtccaggc tcgacccggc ggcgatgtcg gcgtcgtcgg gaaccccgtt
     1261 gccgttgcag tcgaagccgt ggcagccggc ggcataggtg acggagcgga tgatcccgac
     1321 cacccggcgg tgaaaacgca ggtcgatatt gcgctggtat ccctggaaag tgtggcagta
     1381 gctcatcatg gtgctcaccg acggcgtgcc gttgccgcag tcgtcgatgt gcgggttgta
     1441 ctggtcgtcg tgcgtgtggt acgtgccgca gttgtggccc atttcgtggg ggaatacatt
     1501 gatgtcccag ttgccgttgt cggggtaggt gacgggggcc gtgaagctgc cgttgagcag
     1561 ggcgtcgatt ccgtaggcgt tgccgttgca ggtattcgag atgaaggaca cgccgccgta
     1621 gggcgtgtcc cgccgcccgg aaaacatgtg aatgatgttg aacggcgagg ggtcttcgtt
     1681 cacctgccag tagtcgcgga atccgtagag atcctggggg gagaagggtt cgccgccgga
     1741 cggccagagg cgggcgaaac gcagccacag gcgcaggtcg aggtcgcgca cgtagatggc
     1801 gctcgccgcg ccggccagct gcacgatgta gtcgcgcgcc tccacggcgc tgccgaacat
     1861 gttgacatag gcctggtcgg cgtcaatcgc cagatacagc agtttcggtc cggccgacgt
     1921 cttgagcgcc gcttccttga ccatctccag atcgtcgtcg aacgcgctcc ccggttccgt
     1981 gccgcagaag ggcacttcga gttcgccgat gccggcggcc tcgcggatgg tcaccgtgcg
     2041 atcgcccgat ttcagggagg cggacggcgt cgccagcaga tagcgcgtcc ccgccgcgtc
     2101 gatgaaaccg ttgatcatgc cgctcccgga gacggcgagg aaagccgagg agtactcatc
     2161 gccggcgatc cggccgcggt agaggaggac gtcggacggg ggcaggtcgg cgccgccgcc
     2221 ggggcccgcg ataacgaacc gggcgtccgg cgccacgacg tcgaagcgct ccagttccag
     2281 cagcgccgcc tcgccggtcg gcagcggaac ctcgagccgc tgagggccgg cggctccggc
     2341 cagcgcggcg tggagacccg gcgccgactc ggcctgcaac tgccgcgcct cggcgtcggt
     2401 cgttcgaacg ctgaaggggc ggtgaatatc gccgatcggc ggggcggtct cggcggaggc
     2461 aaggcccggg atcaggagca gtcccgcggc cagcgccgac agcaacgcat gccgacggcg
     2521 gagtcgggcg cgaacacggc ctttcatggc accatccttt ccatacttat cgtcgcttta
     2581 ttcttttcat tcgatggacg cggacacgga ctagagacgg tactcccggc ggtcggggag
     2641 gcgagcctgg ccccggggcg gggtcgatag gaacctctac atgtacaagt atagtctcat
     2701 ctcccccgtc cgtcaacgac aatcacgggc ggcgggccgg cggacccgcc cactcgcgcg
     2761 cgagcagccc gtagacggcc acatcgtacc agcggcgccg gaggcggcag tccttgcgcc
     2821 gggtcccctc gtaccggaac ccgactttct ccagcaatct ggcagagccg acgttggggg
     2881 ccaggacgag ggcggtgagg cggtggaggc ggaggtcgcg gaaggcgaag aaatcgcgtc
     2941 cggtatggtt gagaacagcg tcgaggacga cggcgatgcc tgcgtcgtgg aaagagcgga
     3001 cgagagcggc gaggtcggca tcggtgccga gcctccggtc gacatgccag tagtcgacgg
     3061 tgtcgtagcc gtgtgctgcc gactcgaaaa gggggccgag gtatacggcg ttgacgccga
     3121 gggactggag gtgggggatt ctgtccgcta gctgcttgag gccgctgccg ggaggcgagg
     3181 cgaagtcgtt gc
//
LOCUS       DAOOKS010000159         3173 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_37095_length_3173_cov_3.381013, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000159 DAOOKS010000000
VERSION     DAOOKS010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37095_length_3173_cov_3.381013"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2916)
                     /gene="secA"
                     /locus_tag="PK112_03460"
     CDS             complement(<1..2916)
                     /gene="secA"
                     /locus_tag="PK112_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HPC11141.1"
                     /translation="MANLLTRIFGSKHDRDIKKIAPLVAEINEHYERLSVLTEEELTG
                     KTAEFRQRLAEGATLDDLLPEAFAVVKEACRRHVGKTLEVVGLPVEWNMVPFDVQLIG
                     GIVLHQGKIAEMATGEGKTLVATLPTYLNALAGKGVHIVTVNDYLARRDSEWMGWIYT
                     YLGLTVGCIQNQMTNAERRTQYLCDITFGTANEFGFDYLRDNMAQRVEDRVHRNFGYA
                     IIDEVDSILIDEARTPLIISGQVETTTVHDRYREMLATVETLVRKQTRLINDMVAEAE
                     EILAAEDGDHFQAGTLLLAARRGAPKNKRLLKVEKEPGVAKLITDVESAYMRDKKLHE
                     IDDRLYFFIDEREHTIALTDLGTAQLTKEEQNLFELPDLSSMLSEIEGDQSLAPEERQ
                     KRIDAAYAVHAERSEKVHAINQLLRAYSLYEKDVEYVLQDGKVMIVDQFTGRILSGRR
                     YSDGLHQAIEAKEGVKIEAETQTLATITLQNYFRMYEKLAGMTGTAETEAAEFWDIYK
                     LDVTVIPTNKPVIRKDMDDQIFRTRREKYNAIIEEIQTQYNAGRPALVGTTSVEVSET
                     LSRMLKRTGIPHNVLNAKQHQREAEIVAQAGQRSAVTIATNMAGRGTDIRLGPGVVEL
                     GGLHIIGTERHEARRIDRQLRGRAGRQGDPGSSLFFLSLEDHLMRLFGGDRLGAIMDR
                     LGVQEGEVITHGMVTRAIERAQKKVEAQNFAIRKHTLEYDDVMNVQRKWIYERRLAAL
                     ERPSIQEEVVELIEGVLDDLLAEYAPEKDHPEEWNWTGLRDKLREIYLLNFEVRTDDI
                     AEFTQAKLREQLLGAVMAFYRQKEAAYGPEIMRQLERYAVLSTIDRHWRDHLAEMDEL
                     RTGIGLRAYHQGMGKPIDIYKREAFKMFEDMVVRIDREAVNLVYKLRVNIPEQSRTDR
                     RRVPQNLQTSHADATNMGLGGGRAPGEPVGAGMRGAGPTGAGAGPAGMAPHERDPMAE
                     "
BASE COUNT          459 a         1096 c          985 g          633 t
ORIGIN      
        1 ctccgccatc gggtcccgct cgtgcggggc catgccggcc gggccggcac ccgccccggt
       61 cggaccggcg ccgcgcattc ccgcgcccac cggttctccc ggagcgcgcc cgccgcccag
      121 tcccatgttg gtggcgtcgg catggctggt ctgaagattc tgcgggaccc gccggcggtc
      181 ggtgcgcgac tgctcgggga tgttcacccg cagcttgtac acgaggttga cggcctcgcg
      241 gtcgatccgc accaccatgt cctcgaacat cttgaaagcc tcgcgcttgt agatgtcgat
      301 cggtttgccc atcccctggt ggtaggcgcg cagcccgatc cccgtgcgca gctcgtccat
      361 ctcggccaga tggtcgcgcc agtggcggtc gatcgtcgac agcaccgcgt accgctccag
      421 ctgccgcatg atctccgggc cgtaggcggc ctccttctgc cggtagaacg ccatcaccgc
      481 cccgaggagc tgttcgcgga gtttcgcctg ggtgaactcg gcgatgtcgt ccgttctgac
      541 ctcgaaattg agcaggtaga tctcgcgcag cttgtcgcgc aggccggtcc agttccactc
      601 ttcggggtga tccttctccg gggcgtattc cgccagaaga tcgtcgagca ccccctcgat
      661 gagctcgacc acttcctcct gaatggacgg ccgctcgagc gcggcgaggc ggcgctcgta
      721 gatccacttg cgctggacgt tcatgacgtc gtcgtactcc agcgtgtgtt tgcggatggc
      781 gaaattctgg gcctcgacct tcttctgggc gcgctcgatc gcccgcgtga ccatcccgtg
      841 ggtgatcact tccccctcct gtacgccgag gcggtccatg atcgcgccga ggcggtcgcc
      901 gccgaacagc cgcatgaggt ggtcttcgag cgagaggaaa aacagcgagg atccggggtc
      961 gccctggcgg ccggcgcggc cgcggagctg gcggtcgatg cggcgggcct cgtgccgttc
     1021 ggtgccgata atgtggaggc cgcccagttc gaccacgccc gggccgaggc ggatgtcggt
     1081 gccgcggccg gccatgttgg tggcaatcgt caccgcgctc cgctgcccgg cctgggcgac
     1141 aatctcggcc tcccgctggt gctgtttggc gttcaacacg ttgtgcggga tccccgtccg
     1201 cttcaacatc cgcgaaagcg tctccgacac ttccaccgag gtcgtgccga ccagggccgg
     1261 ccggcccgcg ttgtattggg tctggatctc ctcgatgatc gcgttgtact tctcccgacg
     1321 cgtccggaaa atctgatcgt ccatgtcttt acggatcacc ggcttgttgg tcgggatcac
     1381 ggtcacatcg agcttgtaga tgtcccagaa ctcggccgcc tcggtctcgg ccgtcccggt
     1441 catgcccgcg agcttctcat acatgcggaa ataattctga agcgtgatgg tcgcaagcgt
     1501 ctgcgtctcc gcctcgatct tgacgccctc cttggcctcg atcgcctggt ggaggccgtc
     1561 ggagtaccgc cgccccgaca ggatgcgccc cgtgaactgg tcgacgatca tcactttgcc
     1621 gtcctgcagc acgtactcga cgtccttctc gtacagcgag tacgcacgga gcagttggtt
     1681 gatcgcgtgc actttctcgg accgctcggc gtggaccgcg taggcggcgt cgatccgctt
     1741 ctgccgctcc tctggcgcca gcgactgatc cccctcgatc tccgacagca tactcgacag
     1801 gtcgggcagc tcgaacagat tctgctcttc cttcgtcagt tgggcggtgc cgaggtcggt
     1861 caaggcgatc gtgtgctcgc gttcgtcgat gaagaaatag agccggtcgt cgatctcgtg
     1921 cagtttcttg tcgcgcatgt aggcggactc gacgtcggtg atgagcttgg ccacccccgg
     1981 ctctttctcc accttgagca ggcgcttgtt cttcggcgcg ccccgccggg cggccagcag
     2041 gagagtgccc gcctggaaat gatccccgtc ctcggcggcc aggatctcct ccgcctcggc
     2101 caccatgtcg ttgatcaggc gcgtctgctt gcgcaccagc gtctccactg tcgccagcat
     2161 ctcccggtag cggtcgtgga cagtcgtggt ctcgacctgc ccggagatga tcagcggcgt
     2221 gcgcgcctcg tcgatcagga tcgagtcaac ctcgtcgatg atcgcgtagc cgaaattccg
     2281 gtgcacccgg tcctccaccc gctgcgccat gttatcgcgg aggtagtcga agccgaattc
     2341 attggcggtg ccgaaggtga tgtcgcagag gtactgggtg cggcgttccg cgttggtcat
     2401 ctggttctga atgcacccca ccgtcagccc aaggtacgtg taaatccacc ccatccactc
     2461 ggagtctcgg cgggcgaggt agtcgttgac cgtgacgatg tgcacgcctt tgccggccag
     2521 cgcgttaaga taggtcggca gggtcgccac cagcgtcttc ccttcccccg tcgccatctc
     2581 cgcgatcttc ccctggtgga gcacgatgcc gccgatcaac tgcacgtcga acgggaccat
     2641 gttccattcg accggcaggc cgaccacctc cagggtcttc ccgacgtggc ggcggcaggc
     2701 ctccttgacg accgcgaagg cctcgggcaa gaggtcgtcg agcgtcgccc cctcggccag
     2761 ccgctgccgg aactccgccg tcttgcccgt cagctcctcc tcggtcagca cagagagtct
     2821 ctcgtagtgc tcgttgatct cggccaccag cggggcgatc ttcttgatgt cgcggtcgtg
     2881 tttggagccg aaaatcctgg tcagcaaatt cgccatacct gcattcgccg cttcgggttt
     2941 accttaacct tatacaataa tacatgggcc cttccggggc aatcgtcttg ctggagcata
     3001 cgccccggcg gacgcgggag caagataaag cgccccccgg cccccgcact tcgtcgttga
     3061 ccggcccgcc gccgggccgc tatcctgtcg gggcggcggc gcaccgccga cccggccgcg
     3121 cgccggcggc gccgccccga aaaaccctgt atgactgcgc caaccgaccg ccc
//
LOCUS       DAOOKS010000160         3173 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_37098_length_3173_cov_2.635022, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000160 DAOOKS010000000
VERSION     DAOOKS010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37098_length_3173_cov_2.635022"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="PK112_03465"
     CDS             complement(<1..343)
                     /locus_tag="PK112_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11142.1"
                     /translation="MSTYMKRFVYMSLLYLAAGALCGILSAGGSPGYAAVFAHTHFNL
                     LGFMAMIVFGIGYFILPRFNGAELRWPAWVPVHFWLGNVSLVGMVLFRGLYSRDGSNL
                     WHVLFLAAAAVQ"
     gene            546..1313
                     /locus_tag="PK112_03470"
     CDS             546..1313
                     /locus_tag="PK112_03470"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HPC11143.1"
                     /translation="MGSETGKIGKTVGAGNARVATGLTVALAIAVAAVILPKLALSGA
                     VARILTTQALELGLSLGAIAVFGRGRFANYGFRLPRPDRHWSAALAGWMPIGLTALGL
                     GTLATAAMLACGGKGNPLVRELTFPQVILFVWIFSSTIEEVFTRGFLQSHIASAGEPR
                     LGPRFLRIGRAALISAAFFAAMHLVLLLSHTDAVTVVIILLFTFSVGLLAAHQRERTG
                     SLVPAIGVHMLANVGGVFGGIVYAIIAMLSGGAVPGR"
     gene            complement(1350..2747)
                     /gene="asnS"
                     /locus_tag="PK112_03475"
     CDS             complement(1350..2747)
                     /gene="asnS"
                     /locus_tag="PK112_03475"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HPC11144.1"
                     /translation="MDYKVRTRIRRIVVDEQVPVGTTVTILGLVRTIRRSKEVAFVEV
                     NDGSCMQNLQGVVSDPDRIPAIDRILTGAAVRLRGKLIESPAQGQKYEVAVEALDLVG
                     EADATYPIQKKRHSFETLRDMAHLRPRTNTFGAVNRIRSRLAYAIHTYYQERGFYYIH
                     TPIITASDCEGAGELFRVTTLPLDQPPKKDGRVDWEADFFAAEAYLSVSGQLEGELLA
                     AALGDIYTFGPTFRAENSNTARHASEFWMVEPEMAWAELEDDMDLAEDFLKYLFRFAL
                     DDCAGDMAFFNQRIDHKLIETLESIVASEFVRVSYTDAIAQLQKSGRKFEFPAEWGLD
                     LQTEHERCLTEVVYKRPVIVYDYPLAIKAFYMRVNDDWKTVRAMDVLVPRVGEIFGGS
                     QREERLDVLQKHMEMKGLTNWENYAWYFDIRRYGSMPHAGFGLGFDRAVMYITGMQNI
                     RDVLPFPRVPRWAKF"
BASE COUNT          541 a          921 c         1140 g          571 t
ORIGIN      
        1 cctggacggc ggcggcggcg aggaagagaa cgtgccagag attgctcccg tcgcgggagt
       61 agaggccgcg aaagaggacc atcccaacca ggctgacgtt gccgagccag aagtggacgg
      121 gaacccaggc gggccagcgc agctcggcgc cgttgaaacg gggaagtatg aaatagccga
      181 tgccgaagac gatcatggcc atgaagccaa gcaggttgaa gtgggtgtgg gcgaagacgg
      241 cggcgtagcc cggagatccg ccggcgctca gaatgccgca cagggcgccc gccgcgagat
      301 agagcaggga catgtagacg aagcgtttca tgtaggtcga catggcagtc tccgaattat
      361 ggttttcgtt ggcgcaatgt cggcctcggg gccggggaga gccttgaccc aggtcaagaa
      421 gccgggggga aagagccgtc atcggggctg cccctcgact ccagccggga atggtgttgc
      481 ggcaaggccg cgggtaccgt attatggccg ccggagcgcg cggccgggaa aggcaggata
      541 ggatagtggg atcagagaca ggaaagatag gaaagacagt tggcgccggg aacgcccggg
      601 tcgctaccgg gttgactgtc gctctcgcga tcgcggtcgc ggcggtcatt ctgccgaagc
      661 tcgcgctgag cggggcggtc gcgcggattc tcaccacgca ggcgctcgaa ctggggcttt
      721 cgctcggggc gatcgccgtt ttcgggcgcg ggcgattcgc caactacggg tttcgtcttc
      781 cccgcccgga tcggcactgg tcggccgcgc tagccgggtg gatgccgatc ggcctgaccg
      841 cgctgggtct gggaacgctc gccacggcgg cgatgctggc atgcgggggg aaaggaaacc
      901 cgctggtgcg agagctgact tttccgcagg tgatcctgtt cgtgtggatt ttctccagca
      961 cgatagaaga ggttttcacg cgcgggttct tgcagagcca tatcgcctcc gccggggagc
     1021 cccggctcgg gccgaggttt ctccggatcg ggcgggcggc gctgatcagc gcggcgtttt
     1081 tcgcggccat gcatctcgtg ctcctgctca gccacacgga tgccgtcacg gtcgtaatca
     1141 ttctcctgtt caccttctcc gtgggattgc tggcggcgca ccaacgggag cggaccggct
     1201 ccctggtccc ggcgatcggc gtgcacatgc tggcgaatgt cgggggcgtg ttcgggggga
     1261 tcgtgtacgc gatcatcgcg atgctgagcg gcggagcggt tcccggccgg tgaggaaggg
     1321 cgggcccgac cgctcgctct ctctgccggc tagaatttcg cccagcgggg gacgcggggg
     1381 aaggggagga cgtcgcggat gttctgcatg ccggtgatgt acatgacagc gcggtcgaaa
     1441 ccgagcccga acccggcgtg cggcatgctc ccgtagcggc gaatgtcgaa ataccaggcg
     1501 tagttctccc agttggtgag gcccttcatc tccatgtgct tctgcagcac gtcgaggcgc
     1561 tcctcgcgct gggagccgcc gaagatttcg ccgacgcggg ggacgaggac gtccatggcg
     1621 cggacggttt tccagtcgtc gttcacccgc atgtagaagg ccttgatcgc gagcgggtaa
     1681 tcgtacacga tcaccggccg cttgtaaacc acctcggtca ggcagcgctc gtgctcggtc
     1741 tggaggtcca gcccccactc ggcggggaac tcgaacttcc ggccggattt ctgaagctgc
     1801 gcaatcgcat cggtgtagct cacgcggacg aactcggagg cgacgatgct ctcgagggtc
     1861 tcgatcagtt tgtggtcgat ccgctggttg aagaaggcca tgtcgccggc gcagtcgtcg
     1921 agggcgaagc ggaagaggta tttgaggaag tcctcggcga ggtccatgtc gtcctcgagc
     1981 tcggcccacg ccatctccgg ctccaccatc cagaactcgc tcgcgtggcg ggcggtgttg
     2041 gagttctcgg cgcgaaaagt ggggccgaaa gtgtagatgt cgccgagagc ggccgccagg
     2101 agctccccct cgagctggcc ggaaacgctc aggtaggcct cggcggcgaa aaagtccgcc
     2161 tcccagtcga cgcggccgtc cttcttcgga ggctggtcga gggggagggt ggtgacgcgg
     2221 aagagttccc ccgccccttc gcagtcggag gcggtgatga tcggcgtgtg gatatagtag
     2281 aagccgcgct cctggtagta ggtgtggatg gcgtaggcga gccgggagcg gatgcggttg
     2341 accgcgccga aggtgttggt ccggggacgc aggtgggcca tgtcgcggag ggtctcgaaa
     2401 ctgtggcgct tcttctggat cgggtaggtg gcatccgctt ccccgaccag gtcgagcgcc
     2461 tcgaccgcga cctcgtactt ctgcccctgg gcgggcgatt cgatcagctt cccgcgcagg
     2521 cggacggcgg ccccggtgag gatccggtcg atggcaggaa tccggtcggg gtcggagacg
     2581 acgccctgga ggttctgcat gcaggagccg tcgttgacct cgacaaaggc gacttccttg
     2641 cttcggcgga tggtgcgcac gaggccgagg atggtcacgg tggtcccaac cggcacctgt
     2701 tcgtcgacga caattcgcct gattctggtc cggactttgt aatccatcgg tgtcttctcc
     2761 gcgtcagttc cggtcacggg gcacatcggg ctgtcggtgg caataaaacg agcggggggc
     2821 ggtttggcaa gtctcaagaa acgggcggcc gggcgcgaaa tcccgcccgc ccaatgaaac
     2881 ttccgccgcg ctcgcaggta ttaaaggtga gaatgaactg agtgtgactc cttcgagaga
     2941 cgttcccaac ttctacatag aaacccggcc tggctgacag gccgggtttt tatttgcgcc
     3001 ggatcgcgcg ccaacccgcc cggctttctc gcccagccgg cggggggtgc ttgacggcgg
     3061 gcggcgggaa accgagattg cgccatgtat aaacgtcagt ctgtcgatcc ggccgaggcc
     3121 ggggaggtcg taatatgtcc acacaagatt catcgccgcg gtccgattcc ggg
//
LOCUS       DAOOKS010000161         3167 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_37194_length_3167_cov_2.711118, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000161 DAOOKS010000000
VERSION     DAOOKS010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37194_length_3167_cov_2.711118"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="PK112_03480"
     CDS             complement(<1..888)
                     /locus_tag="PK112_03480"
                     /inference="COORDINATES: protein motif:HMM:NF023462.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3524 domain-containing protein"
                     /protein_id="HPC11145.1"
                     /translation="MHLLALEPYYAGSHRAFVDGWIAGSRHRWTLLTLPGYKWKWRMR
                     HAPLTFCDEIAARRRDGERWDGLFATDMVDLAQLLGLARAALGNLPAVVYFHENQLTY
                     PSLHAGERDYHFAMTNFSTAAVAAGVWFNSDFHRREFLEGLRSFFRRMPDYPPLAWVD
                     RIAARSEVYPQGIAPPRPLSARPPGPLRILWAARWEHDKNPEDFFAAMRALRSRGVDF
                     RLSVIGEQFSEVPAAFAEARREFAPLIDRWGYQPDRADYERALSAADLIVSTARHEFF
                     GVAIVEAVAAGIRPVLPQRL"
     gene            complement(1009..1647)
                     /locus_tag="PK112_03485"
     CDS             complement(1009..1647)
                     /locus_tag="PK112_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11146.1"
                     /translation="MRKNAPLLIALFLATATLAPAVDFGFGLQVDDEGRKSFYLAVSN
                     HYQVPEKEIVVVRERHIPDEEMPVVFFLAARCNASPATIIDLRLGGKSWMDITLQFGK
                     TAEIFYVPVEDVSGPPYGHAYGHFKKHPKNRWGEIRLSDDDVVNFVNLKFICDHYGWT
                     PNEVIRMRQDGRGFIDINRDIKDRKAGAKTQASTGSKDQPAKNKGKGKDKQK"
     gene            1804..>3167
                     /locus_tag="PK112_03490"
     CDS             1804..>3167
                     /locus_tag="PK112_03490"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HPC11147.1"
                     /translation="MRLRFHTRLGAGDYLKFRGVRFGRRGGARSLMTAVAIAAALSGG
                     AAADEGMWPLYELDRLDWDSLQARGLRLSREQMYDTARPDVADACINLGGGSASFVSP
                     KGLIITNHHVAFGAIQRASTVEHNYLRDGFYAPTPAEEIRAVGTSVYVTLAEADVSDR
                     VLAAVPEELDGLARYEALDRITKEIVREAEQGRDDIQAEVTPIYGGTKYTLTTFLRIR
                     DVRIVHAPPMAIGNYGDEIDNWIWPRHVGDYSFLRAYVGPDGKPAEYSRDNVPYEPQA
                     WLPVSAQGVKEGDFTMMIGYPGKTQRYTVARAIAEAVNFTYPRSIDRSLQLLAKIDSA
                     GAENPGIALRMASTVQGISNRMKKTQGLVAGLARDSVVARTAAAEAELTVFLRGDSAR
                     WERFGGLIPAFDSLYDAHAAARERREVLGMVWYVDHLSLARQVYRWAEQREKPDPERE
                     SGYQ"
BASE COUNT          549 a          945 c         1135 g          538 t
ORIGIN      
        1 gaggcgttga gggagcacgg gccggatccc ggcggcgacc gcctcgacaa tggccacgcc
       61 gaagaattca tggcgggcgg ttgatacgat gaggtcggcg gcggataagg cgcgctcgta
      121 gtccgcgcgg tcgggctgat aaccccagcg gtcgatgagc ggggcgaatt cccgccgggc
      181 ctcggcgaag gccgccggta cctcggaaaa ctgctcgccg atcacgctga gacgaaaatc
      241 gaccccccgg gagcggagcg cgcgcatggc ggcgaagaag tcctccgggt tcttgtcatg
      301 ctcccaccgg gcggcccaga ggatccgcag cggtccgggc ggccgggctg aaagcggccg
      361 cggcggggcg atcccctggg gatacacctc cgaacgcgcg gcgatccggt cgacccaggc
      421 caggggaggg tagtcgggca tccgccggaa aaaggaccgc aacccctcga gaaactcgcg
      481 gcggtggaaa tccgagttga accagacgcc cgcggcgacc gctgccgtcg aaaagttcgt
      541 catggcgaag tggtagtccc gctccccggc atggagactg gggtaggtga gttggttctc
      601 gtggaagtag acaacggccg ggaggttgcc gagagcggcg cgggccagcc ccagcagttg
      661 ggcgaggtcg accatgtcgg tggcgaagag accgtcccac cgctcgccgt ctcgccggcg
      721 ggccgcaatt tcatcgcaga aggtcaaggg cgcgtggcgc atccgccact tccacttgta
      781 gcccggcagg gtgagcaagg tccagcggtg gcggctgccg gcaatccaac cgtccacgaa
      841 ggcgcggtgg ctgccggcgt agtatggttc cagggcgagc agatgcatca ccgatctccg
      901 gctgcaagga acgccgccgc ccggcccttg tcaatcgggc tcccccaaaa aagccacggg
      961 ccgccggaga ggcggcccgt gtcggggatg gcggtgttga gggatggatt acttctgctt
     1021 gtctttcccc ttgcccttgt tcttggcggg ctggtctttc gatccggtgc tggcctgtgt
     1081 tttcgcgccc gccttccggt ccttgatgtc gcggttgatg tcgatgaaac cgcggccgtc
     1141 ctggcgcatc ctgatgacct cgttcggggt ccagccgtag tggtcgcaga tgaatttgag
     1201 gttgacgaag ttcacgacat cgtcgtcgct caggcggatt tcaccccacc ggtttttcgg
     1261 atgcttcttg aagtggccgt aggcgtggcc atagggcggg cccgacacgt cctcgaccgg
     1321 cacatagaat atctcggcgg tcttgccgaa ctgcagggtg atgtccatcc aggacttgcc
     1381 gccgaggcgc aggtcgatga tggtcgccgg ggaggcgttg cagcgggcgg ccaggaagaa
     1441 gacgaccggc atctcttcat cgggaatgtg ccgctcgcgg accaccacaa tctctttctc
     1501 cggaacctgg tagtggttgc tgactgccag gtagaaactc ttgcgccctt cgtcatccac
     1561 ctggaggccg aagccgaagt cgacggctgg ggccagggtg gcggtcgcaa gaaacagggc
     1621 gatcagcagg ggggcgtttt tgcgcatatc atcctccagg gaaggtccgg tgtacctgat
     1681 aggtatatcg gctgggcggg cgcggtaatt gagcagcgaa ttcgaccgcc cggccaagga
     1741 ttagcgcttg ccagttttgg tgtcatgagc cagattgctg gaaacctgag caggagttgg
     1801 cgtatgcgtc ttcgtttcca caccaggctc ggcgcggggg attacctgaa gttccgagga
     1861 gttcgcttcg gccgtcgcgg gggcgctcgc agcctgatga cggcagtcgc gatcgcggcc
     1921 gctctgagcg gcggggccgc cgccgacgag gggatgtggc cgctgtatga gctcgaccgc
     1981 cttgattggg acagcctcca ggcccggggg ctccggttgt cgcgggagca gatgtacgac
     2041 actgcgcggc cggatgttgc ggatgcctgc atcaacctcg ggggcgggag cgcctccttc
     2101 gtctcgccga aagggctgat catcacaaac caccacgtgg ctttcggggc gatccagcgc
     2161 gcctcgacag tcgagcacaa ctaccttcgg gacggcttct acgctcccac gcccgcggag
     2221 gagatccgcg cggtgggaac gagcgtgtat gtcacgctgg cggaggcgga cgtgagcgac
     2281 cgggtgctgg cggcggtgcc ggaggaactc gacggcctgg cgcgctatga ggcgctcgac
     2341 cggattacga aagagattgt ccgggaagcc gagcagggcc gggacgacat ccaggcggag
     2401 gtgaccccga tctacggcgg gacgaaatac acgctgacaa ccttcctgcg catccgcgac
     2461 gtccgcattg tgcacgcgcc gccgatggcg atcgggaact acggcgacga gatcgacaac
     2521 tggatctggc cccgccacgt gggagactat tccttcctcc gggcatacgt ggggcccgat
     2581 ggaaagccgg ccgagtattc ccgggacaat gtcccgtacg agccgcaggc gtggctgccg
     2641 gtgtcggcac agggagtcaa ggagggggat ttcacgatga tgatcggtta tccggggaag
     2701 acgcagcggt acacggtggc gcgcgccatc gccgaggcgg tgaacttcac ctacccgcgg
     2761 tcgatcgacc ggtccctgca gctgttggcg aaaatcgatt cggcgggagc ggagaatccc
     2821 gggatcgctc tgcgcatggc ctcgacggtg caggggatca gcaaccggat gaagaagacg
     2881 caggggctgg tggccggtct ggccagagac agcgtcgtgg ccaggacggc ggccgccgag
     2941 gccgaattga cggttttcct gcgcggcgat tccgcgcgct gggagcgttt cggcggtttg
     3001 atcccggcat tcgacagtct ctacgatgcc cacgccgctg cgcgcgagcg gcgggaagtg
     3061 ctcggcatgg tctggtatgt cgatcacctc tctctggccc ggcaggtgta tcgatgggcg
     3121 gagcagcggg agaaaccgga ccccgagcgc gagagcggtt accagga
//
LOCUS       DAOOKS010000162         3156 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_37355_length_3156_cov_3.068043, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000162 DAOOKS010000000
VERSION     DAOOKS010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37355_length_3156_cov_3.068043"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            198..>3156
                     /locus_tag="PK112_03495"
     CDS             198..>3156
                     /locus_tag="PK112_03495"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPC11148.1"
                     /translation="MGHGTHCAGIVAGAIGNGIGIAGAVADVRIMALKFYPYMPLSAA
                     AAGIVYAADNGADVISMSWGLAGQIPVVEEALGYAAARGVVLIASIGNDGIEQINYPA
                     SYDLTVAVGATNDSDHVTSFSTISPYIDVCAPGRSILSLRAAGTDMYAPSEPNVHIVD
                     SQYYIASGTSMSGPYTAAVAAYVRSLSPGLDRERVREILHATADDYVDPYGTGASYPG
                     WDKYSGWGRVNLASAIAAAPDVRAIITSPGEFEIAAGTIAILGTADGADFTEYRLQYR
                     PAAADTSAWTDLLTSTVPVTAGWLGDWNVSGLPTAEYVLRLQVGQWNRDMVRVSVGGA
                     ALAEIASPGPGDTVNGGVTVTGTCFAPDFVRCVIDYGAGLNPTEWHAVDTTTRVVFAG
                     ELGRWDVSLLDDGLYTLRVQVYGAAGPIAGDEAVVQVYSPFAEPLGWSLDFDRKIGRI
                     ATYADVDGDGVNEIILGTATNVVFLTPDGTLKTTGVPTLEGGDFTHVMPAVGRLDGDD
                     AEDFVVMDLSGKMYICHGLSTEPTVVSLNAPTMTTASYGSSANAPVLFLRDLDGDGID
                     EINYSAGLPSSGSFRIVRADGSPWLWCGADSTAPSGYQECHPADLDGDGVCEIYCYGT
                     VLRKFDAQGCSAEGDTVALVQDGLSIGRAGLSLSSADLDADGDEELIVLGDSWIDPVT
                     PVDHAIYAIDENLQSVAGWPHAMGLGAYIPVLEPAFADLDGDGALEYVCANWNAIRAW
                     RLDGSPLMGDSASFGYFCSSMEPGVVDHLVIGDVTGDYRPDVAALIGGDFLSGYEVSR
                     IEAYSDIGALQPGFPMTITSDGSGFSRCPTLCDLDKDGYLDVICPSQNGINGRLVLHR
                     FAGVRYRPDDRHFFDEKYFNNDGLTDVTFRGDARQEAELPIKLTGLNIVPFGSIRGSY
                     WDGQPLDEGGLWRGLGLYGVRGSGWLSRVYDNIDSELLDIHRIRHIMQPDFAAWGAHS
                     NTRSDNITPFDEGI"
BASE COUNT          522 a          921 c         1124 g          589 t
ORIGIN      
        1 ttgtcgacac cggggtggac tgggatcacc cggacctggt gtcacggatg tggcagaacc
       61 cgggggagat tgccgacaac gggatcgacg acgatcggaa cggctatatt gacgacctcg
      121 tctgatggga tttcagcgcg aatgacctgg gcgatccgcc gtttgcgatc tgggaggata
      181 atgattcgac cgatccgatg ggtcacggca cccactgcgc cgggattgtg gccggggcga
      241 tcggcaacgg tatcgggatc gccggggcgg tcgcggatgt acggatcatg gcgctcaagt
      301 tctatcccta catgccgctc tcggcggcgg ccgccggcat cgtgtacgcc gccgacaacg
      361 gggccgacgt gatcagcatg agctggggcc ttgcgggcca gataccggtg gtggaggagg
      421 ctctcggtta tgccgccgcc cgcggcgtgg tgttgatcgc cagtatcggc aacgacggga
      481 tcgagcagat caactacccg gcgagctatg acctcacggt tgcggtgggg gcgaccaatg
      541 actcggatca cgtcacctcc ttttcgacga tcagtccgta catcgatgtg tgcgctccgg
      601 ggcggtcgat tctctcgctt cgggcggccg ggacggatat gtacgcgccc agcgagccga
      661 atgtccacat cgtcgacagc cagtactata tcgcgtcggg gaccagcatg tcggggccgt
      721 acacggcggc cgtggcggct tacgtgcggt cgctgtcgcc cggcctggac cgggagcgcg
      781 tgcgggaaat cctgcacgcc accgccgatg attacgtcga tccgtacggg acgggggcaa
      841 gctaccccgg atgggacaaa tatagcgggt ggggacgggt caatctggct tcggcaatcg
      901 ccgcagcgcc ggacgtgagg gccataatca cgagtcccgg agaattcgag atcgcggcgg
      961 gaacgatcgc gattctcggc accgccgacg gagcggattt cacagaatac cggctgcagt
     1021 accgcccggc ggcggcggat acgtcggcat ggaccgatct gctcacatcg accgttcccg
     1081 tcaccgccgg gtggctgggt gactggaacg tgtccgggtt gccgacggcc gagtatgttc
     1141 ttcggctgca ggtcggccag tggaaccggg acatggtgcg cgtgtcggtc ggcggcgcgg
     1201 ccctggccga gatcgcctca cccgggccgg gggacacggt caacggcggc gtgacggtca
     1261 cgggaacctg ttttgcgccg gatttcgtgc gctgtgtcat cgactacggc gccggcctca
     1321 acccgacgga gtggcacgcc gttgacacga cgacccgggt cgtgtttgcg ggcgaactcg
     1381 ggcggtggga tgtttcgctg ctggacgacg gtctgtacac gctccgcgtg caggtgtacg
     1441 gcgcggccgg tccgattgcc ggcgacgagg cggtcgtgca ggtgtactcg ccgttcgcag
     1501 agccgctcgg atggtccctt gattttgacc ggaagatcgg ccgcatcgcc acctatgccg
     1561 atgtcgacgg cgacggcgtg aacgagatta tcctggggac ggccaccaat gtcgtgttcc
     1621 tgactccgga cgggaccttg aagacgaccg gtgtgccgac gcttgagggc ggggatttca
     1681 cccacgtgat gccggcggtg ggccgactcg acggggatga cgccgaggat ttcgtcgtca
     1741 tggatctgag cgggaagatg tatatctgcc atggtctgtc cacggagccg accgtggtct
     1801 cgctgaatgc tccgacgatg acaaccgcct cgtacggttc gtccgccaat gcgccggttc
     1861 tgtttctccg cgacctggac ggggacggca tcgacgaaat caactactcc gcggggctcc
     1921 cgtcgtcggg gagcttccgc atcgtcaggg cggacggcag tccctggctc tggtgcgggg
     1981 ccgattcgac ggcgccgagc ggataccagg aatgccatcc ggccgacctg gacggcgacg
     2041 gcgtctgcga gatctactgc tatggcacgg tgctgcggaa gttcgatgcc caggggtgtt
     2101 cggccgaggg ggacacggtg gcgctggtgc aggacgggtt atccataggg cgcgccggtc
     2161 tgagcctctc gtcggcggac ctcgacgcgg atggggacga agagctgatc gtgctggggg
     2221 attcctggat cgatcccgtg acgccggttg atcacgcgat ctacgcgatt gatgagaatc
     2281 tccagtcggt ggcgggatgg ccgcacgcga tgggcctggg cgcgtacatc ccggtgctgg
     2341 agccggcgtt tgccgatctg gacggcgacg gcgcactgga gtatgtctgc gcgaactgga
     2401 acgcgatccg ggcgtggcgg ctggacggca gcccgctcat gggagacagc gcgagcttcg
     2461 ggtacttctg ctcctcgatg gaaccggggg ttgtcgatca cctggtcatc ggagatgtga
     2521 ccggcgacta ccggccggat gtcgcggcgc tgatcggcgg ggattttctg tcggggtatg
     2581 aggtgagccg gatcgaggcg tattcggaca tcggcgcact tcagccgggc tttccgatga
     2641 cgatcacgtc ggacggcagc gggttctctc gctgtccgac gctctgcgat ctggacaagg
     2701 atgggtacct ggacgtcatc tgtccttcgc agaacggcat caacggacgt ctcgtgctgc
     2761 accggttcgc cggcgtgcgc tatcgcccgg atgatcggca cttcttcgac gagaagtatt
     2821 tcaacaacga cgggctgacc gatgtgacct tccgcggcga cgcacgtcag gaggccgaac
     2881 tgccgatcaa gttgacgggg ctgaacatcg tgcccttcgg cagcattcgc ggcagttatt
     2941 gggatggtca gccgctggat gaaggcggtt tgtggcgcgg actcggcctg tatggcgtcc
     3001 gcggcagcgg ctggctttcg agggtttatg acaacatcga ttcggagttg ctcgacatcc
     3061 atcgcattcg ccacatcatg cagccggatt tcgcggcgtg gggcgcgcac agcaacacgc
     3121 gcagcgacaa catcacgccg tttgacgaag gcattg
//
LOCUS       DAOOKS010000163         3152 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_37416_length_3152_cov_3.205360, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000163 DAOOKS010000000
VERSION     DAOOKS010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37416_length_3152_cov_3.205360"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1117)
                     /locus_tag="PK112_03500"
     CDS             complement(<1..1117)
                     /locus_tag="PK112_03500"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPC11149.1"
                     /translation="MSKNTTADASRSNARNPAKRSSRSRCPPPPNDRIYHARIGQTKA
                     NSLVDDEIDSLKSHILFLEKRGYAVEGAVSGDDAVELVRRKSFDLVLLDEMMPGKDGL
                     TTLEEIKEIRPHLPVVMVTKSEEETLMDQAIGQKIDDYLTKPVNPTQILSVIKRMLEA
                     KRIISSSTMQRYVTEMNRFNQKLFGRLEPEDWYEAGRVLAAWNLELDRHADVGLEQAL
                     EGSRKEWNTEFTKYLDRNYLDWLHSDRAPTLSPRLAERFLFPHLREKRKLVFIVVDCM
                     RLDQWLMVEPLIAEYYDVRRDYYFSILPSATPFARNALFAGLFPDEIAQARPDLYRTQ
                     AEGSLNRLEDELFEDNLQRHGFDQARQMKYVKIYNNTE"
     gene            complement(1020..2276)
                     /locus_tag="PK112_03505"
     CDS             complement(1020..2276)
                     /locus_tag="PK112_03505"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPC11150.1"
                     /translation="MPKPLSRFSLTNRLYVGKSTFYNGFLLVGVVAISLVFIWYTLNV
                     IDRLKEGTRSQVEKYVKMWQLAANSNTSGEELQFIFDEIIVKADFPIIVLDQNREPIH
                     WRNIKGIGPTENSPKALATLRRLAESMAARNQEFPLHFGEGHVNYFLYGDTDAITRLR
                     YMPFVEISIVLAFLAVALVGYHNIRRSEERHIWVGMAKETAHQLGTPISSLMGWIEVM
                     RAECDPAADRAQLQAQFADGLENMRIDIDRLQKVANRFGQIGSIPELHPADVNRAVAD
                     AVEYFRRRLPFEGQGITLDVRRGELPPVDLNPELFGWAIENLIKNSLQAVDPKAGRVL
                     FTTSYLPHRQCVVIDVEDNGAGIPAAAQRRIFRPGFTTKKRGWGLGLTLVRRIVEEYH
                     RGRVTLKRSKPGETVFEITLPASTQR"
     gene            complement(2276..>3152)
                     /gene="serS"
                     /locus_tag="PK112_03510"
     CDS             complement(2276..>3152)
                     /gene="serS"
                     /locus_tag="PK112_03510"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HPC11151.1"
                     /translation="PAPAFPVRPHWEIGEQLGILDIAAATKVSGAGFYALRGLGARLE
                     RALIAYMLDTHAAGGFCEHAAPYLVTEETMFGTGQLPKMTEDMYRTEDNMWLIPTAEV
                     SLTNLFRQEILDYRRLPILVVGHSPCFRREAGAAGKDTRGMIRVHQFHKVEMVKIVRP
                     ETSYDELESLVAQAEKILQGLRIPYRVGLLASADLSFAAAKCYDIELWAAGIERWLEI
                     SSCSNFEDFQARRMNCRFRDEDKKVRFPHTLNGSGLALARLVPAILENYQNADGSVTV
                     PEVLRPYMGGLARIG"
BASE COUNT          507 a          838 c         1193 g          614 t
ORIGIN      
        1 cctcggtgtt gttgtagatc ttgacgtact tcatctggcg cgcctggtcg aagccgtggc
       61 gctggaggtt gtcctcaaag agttcatcct cgaggcggtt gagcgagccc tcggcctggg
      121 tccggtagag gtcggggcgg gcctgggcga tttcatcggg gaacagcccg gcgaagaggg
      181 cgttgcgggc gaagggggtg gccgagggga ggatggagaa gtagtagtcg cggcggacgt
      241 cgtagtactc ggcgatgagc ggctcgacca tcagccactg gtccaggcgc atgcagtcga
      301 cgacgatgaa gacgagcttg cgcttctcgc ggaggtgggg aaagaggaag cgttcggcca
      361 ggcgcgggga cagggtcggg gcgcggtcgc tgtggagcca gtcgaggtag ttgcggtcga
      421 ggtatttggt gaactcggtg ttccactctt tgcgggagcc ctcgagggcc tgttccagtc
      481 cgacgtcggc gtggcggtcg agttcgaggt tccaggcggc gaggacgcgg ccggcctcgt
      541 accagtcctc gggctcgagg cggccgaaga gtttctggtt gaagcggttc atttcggtga
      601 cgtagcgctg catggtgctg ctgctgatga tccgcttggc ctcgagcatg cgcttgatga
      661 ccgacaggat ctgggtcggg ttgaccggtt tggtgaggta atcgtcgatc ttctgcccga
      721 tcgcctggtc catgagagtc tcttcttcgg acttggtgac catgacgacg gggaggtggg
      781 gccggatttc cttgatctcc tcgagggtcg tcaggccgtc cttgccgggc atcatttcgt
      841 cgaggaggac gaggtcgaag gatttgcggc gcacgagctc gacggcgtcg tcgcccgaca
      901 ccgcgccttc caccgcgtag ccgcgttttt cgaggaagag gatgtgcgac ttgagggagt
      961 cgatttcatc atcgaccaga gaattcgctt ttgtttggcc gatccgggca tggtagatcc
     1021 tatcgttggg tggaggcggg cagcgtgatc tcgaagaccg tttcgccggg tttcgagcgt
     1081 ttgagcgtga cgcgtccgcg gtggtattct tcgacaatgc ggcgcacgag ggtcaggcca
     1141 aggccccacc cccgcttctt ggtggtgaat ccggggcgga agatgcggcg ctgggcggcc
     1201 gccgggatgc cggcgccgtt gtcctcgaca tcgatcacca cgcactgccg gtgggggagg
     1261 taggaggtgg tgaagaggac gcgcccggct ttgggatcca ccgcctggag ggaattcttg
     1321 atgagatttt cgatggccca tccgaagagt tcggggttga gatcgaccgg ggggagctcg
     1381 ccgcggcgga cgtcgagggt gatgccctgg ccctcgaagg gcaggcggcg gcggaagtac
     1441 tcgacggcgt cggcaaccgc ccggttgacg tcggccgggt gcagctcggg gatggacccg
     1501 atctggccga agcggttggc gactttctgg agccggtcga tgtcgatgcg catattctcg
     1561 agaccgtcgg caaactgcgc ctgcagttgg gcgcggtcgg cggccgggtc gcactcggcc
     1621 cgcatgacct cgatccagcc catgagcgag gagatcggcg tgccgagctg gtgggcggtc
     1681 tccttggcca tgccgaccca gatgtggcgt tcctcgctgc ggcggatgtt gtggtagccg
     1741 acgagggcga cggcgaggaa agcgaggacg atgctgattt cgacgaaggg catgtagcgg
     1801 agccgggtga tggcgtcggt gtcgccgtag aggaagtagt tgacgtggcc ctcgccgaag
     1861 tgcaggggga attcctggtt gcgggcggcc atgctctcgg ccaggcggcg gagggtcgcc
     1921 agcgccttag gcgagttctc ggtcggcccg atgcccttga tgttgcgcca gtggatcggc
     1981 tcccggttct ggtcgaggac aatgatcggg aaatcggctt tcacgatgat ttcgtcgaag
     2041 atgaactgga gttcctcgcc cgatgtgttg gagttggcgg caagctgcca catcttgacg
     2101 tacttctcca cctggctgcg ggtgccctcc ttgaggcggt cgatgacatt gagcgtatac
     2161 cagatgaaga cgagcgagat ggcgaccacg ccgaccagga ggaagccgtt gtagaaggtc
     2221 gacttgccga catagagccg gttggtcaga ctgaatctgc tcagcggttt gggcatcagc
     2281 cgatcctggc gaggcctccc atgtagggcc ggaggacttc ggggacagtc acggaaccgt
     2341 cggcgttctg gtagttttcg agaattgccg ggacaaggcg ggcgagggcc agtccgctcc
     2401 cgttgagggt gtgcggaaag cgcaccttct tgtcctcgtc gcggaagcgg cagttcatgc
     2461 ggcgggcctg gaaatcctcg aagttggagc aggaggagat ttcgagccag cgttcgatcc
     2521 cggcggccca gagttcgatg tcgtagcatt tggcggcggc gaacgagaga tcggcggagg
     2581 cgagcaggcc gacgcggtag gggatgcgga gcccctggag gattttctcg gcctgagcga
     2641 cgagcgactc gagttcgtcg taggaagttt cgggtcgcac gattttcacc atctcgactt
     2701 tgtggaactg gtggacgcgg atcatgccgc gggtgtcttt gccggccgcc ccggcctcgc
     2761 ggcggaaaca gggggaatgc ccgaccacga ggatcggcag ccggcgatag tcgaggatct
     2821 cctgccggaa caggttggtc agcgacacct cggcggtcgg gatgagccac atgttgtcct
     2881 cggtgcggta catgtcctcg gtcattttgg gcaactgccc ggtgccgaac atggtctcct
     2941 cggtgaccag gtagggagcg gcgtgctcgc agaaaccgcc ggcggcgtgg gtgtcgagca
     3001 tgtaggcgat gagcgcgcgt tcgaggcggg cgccgaggcc gcgcagggcg tagaagccgg
     3061 cgccggagac tttggtggcg gccgcgatgt ctaggatgcc gagctgctcg ccgatctccc
     3121 agtgggggcg gaccgggaag gcgggcgcgg gc
//
LOCUS       DAOOKS010000164         3148 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_37486_length_3148_cov_2.720983, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000164 DAOOKS010000000
VERSION     DAOOKS010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37486_length_3148_cov_2.720983"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="PK112_03515"
     CDS             <1..227
                     /locus_tag="PK112_03515"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPC11152.1"
                     /translation="NPATTISCDLPSKSNVRLTVYNVLGQEVITLIDREMDADQHSVS
                     WDASKQASGVYFYRLEAGSFTETRKMLLLK"
     gene            455..>3148
                     /locus_tag="PK112_03520"
     CDS             455..>3148
                     /locus_tag="PK112_03520"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HPC11153.1"
                     /translation="MLGVIGNRFSIGKETYPPFSAELHYFRIDKRHWSICFERIKRAG
                     FRIIATAVPWNIHQDQTKAFDFAGMTDPRKDLIVFLELAREFGFKVILRPGPWVAGQL
                     PYGGLPKHLFNDLKVFARDAQGQERELPADHGVKAGYLPSYLHPNFQFHLKTYFKALI
                     ETTKNYVHPRGPVFMVELDYETSFGRLLDPASADYNPDVLAAHYPRFLRELYQDDIKK
                     LNAQYRTKYAEFEAVEPPRKFAELEMKDYPPVLDWLRFREYILRTYLQTVEDLFTAYT
                     VEPLIFRSLYIRPGDLLPAYNLVPEEMAPFLGSNVFPEGNYFDLVIKARFLKEEYGFA
                     FASSFVSGRAAADPEREQRIAPVCNNERRFYLAAGFAAGFKGMNHYMFVDREHWYGAP
                     LHHDGTVSDGYRVIQRFNTAITEIGLEEMESRPQIAVVGNRLYSWLQLTDTSKSFAYL
                     PRLLNESTVGFCRDLTRLKLNFGVRENRDWSTMRDYKVLFIPSAEVMAQRDQEAIVEL
                     VKNGQTVIMCGLMPKYDEHFRDCKILSNHFRIKTTVDTRIGTITCKDGAEFPAYLYGS
                     IAAGDDAKTRKTAKCGAKVVGVTSARSKGNFIFFAFDIASGGNHKKLAFIEAVLESEK
                     QVSHLYCSDPSVDVAFHLGSKKSLLYVVAPPAGELSDGFEAKTKEVIIRADLKGLGFG
                     AVKVKLTDLFGDPENPTVLKSSAKELQEGLPLRLSFPDGLVFWSRNDSGESLMRRGGM
                     RRRLGLAAVLLFGVVGPACDGIRVTRLYQEGAAVTAAPLATQVGEEIFRRGGNAFDAA
                     VAIGFALAVVHPQAGNLGGGGFAVIRSAQADTVRTLDFRETAPRAATGGMYLADSVTV
                     IAEASTVGALAAGVPGTVAGLYALWQAYGSMPWEELVRPAAL"
BASE COUNT          577 a          869 c         1051 g          651 t
ORIGIN      
        1 tcaacccggc gacgacgatt tcgtgtgatt tgccgagcaa atcgaatgtc cggttgacgg
       61 tgtacaacgt cctcggtcag gaggtgataa ccctgatcga ccgggagatg gatgctgatc
      121 agcattcggt gagctgggat gcctcaaagc aggcctcggg ggtgtatttc tatcggctcg
      181 aggcgggcag tttcaccgag acccgtaaga tgttgctgct gaagtgacct ctgacggtcc
      241 atagcttcac aaggctactt gaagcccccg tttcgacggg ggctttttct tggcaaaatg
      301 gcccgacctg tcagtttttg ccacctctcg tttaagttat tgttgtgcaa tcagttaaat
      361 ctttcatgtt gactttgtct caggcatgtg atattttgct tttttgcggt agctggaaaa
      421 ttcacgttaa gctgttgata tataaggcgt tacaatgtta ggagtaatcg ggaatcgttt
      481 ctcaattggt aaagaaactt atccgccgtt ctcggccgag ctccattatt tccgaattga
      541 caagcgacac tggtccatct gttttgagcg gatcaagcgg gcgggattcc gcattatcgc
      601 aaccgccgtg ccctggaata tccaccagga ccagaccaag gcgtttgatt ttgcggggat
      661 gacggatccg cggaaggatc tgatcgtttt cctggagcta gcccgggagt ttgggttcaa
      721 ggtgattctt cgaccggggc cgtgggtggc gggccagttg ccctatggcg gtctgcccaa
      781 gcacctgttc aatgatctga aagtgtttgc gcgtgacgca caggggcagg agcgggagtt
      841 gccggccgac cacggggtca aggcgggtta tctcccgagc tacctgcacc cgaattttca
      901 gtttcatctg aagacgtatt tcaaggccct gatcgagacg acgaaaaact atgtccaccc
      961 gcgcgggccg gtgttcatgg tggagcttga ttacgaaacc tcgttcggcc gcctgctgga
     1021 cccggcgagc gccgactaca atccggatgt gctggcggcc cactaccccc ggtttctgcg
     1081 ggagctgtat caggacgaca tcaagaagct caacgcccag taccggacga agtacgcgga
     1141 gttcgaggcg gtcgagccgc cgcggaagtt cgccgagctg gagatgaagg actacccgcc
     1201 ggtgctcgac tggctccggt tccgggagta catcctgcgg acgtatttgc agacggtgga
     1261 ggacttgttc acggcgtaca cggtcgagcc gctgattttc cgttcgctct atatccggcc
     1321 gggggatctc ctccccgcct acaatctcgt gcccgaggag atggcgccgt ttctggggag
     1381 caatgttttt cccgagggga actacttcga tctggtgatc aaggcgcgtt ttctgaaaga
     1441 ggagtacggt ttcgcattcg cctcgtcgtt cgtctcgggg cgggcggcgg ccgatccgga
     1501 gcgggagcag cgcatcgccc cggtgtgcaa caacgaacgg cggttctacc tcgcggccgg
     1561 gttcgcagcc gggttcaagg gcatgaacca ctacatgttc gtggaccgcg aacactggta
     1621 cggcgcgccg ctccaccacg acggcacggt ctcggacgga taccgggtga tccagcggtt
     1681 caacacggcg atcaccgaga tcggtctgga ggaaatggaa tcccggcccc agatcgcggt
     1741 cgtcggcaac cgcctgtatt cctggctgca actgaccgat acgtcgaaat cgttcgccta
     1801 tctcccccgc ctgctcaacg agtcgacggt tggtttttgc cgggacctga cccggctgaa
     1861 gctgaatttc ggcgtgcgcg aaaacagaga ctggtcgacg atgagggact ataaggtact
     1921 cttcattccc tcggccgagg tcatggcgca gcgggaccag gaagcgattg tcgagctggt
     1981 gaaaaacggc cagacggtca tcatgtgcgg gctgatgccg aagtacgacg agcatttccg
     2041 ggactgcaaa atcttgtcga atcatttccg aatcaagacg accgtggaca cgcggatcgg
     2101 gacgatcacc tgcaaagacg gcgcggagtt cccggcgtac ctctacggga gcatcgcggc
     2161 cggggacgac gccaagacgc gcaagacggc caagtgcggg gcgaaggtcg tgggggtgac
     2221 ctcggcccgg tcgaaaggga acttcatctt cttcgctttc gacatcgcct cgggcggcaa
     2281 ccacaagaag ctggccttta tcgaggcggt gctcgagtcg gaaaagcagg tgtcgcacct
     2341 gtactgctcc gatccctcgg tcgacgtggc gttccacctg gggtcgaaga agtcgctgct
     2401 gtacgtggtg gcgccgccgg ccggggagct gtcggacggg tttgaggcga agacgaagga
     2461 ggtcataatc cgggcggacc tgaaggggct ggggttcggt gcggtgaaag tcaagctcac
     2521 cgacctgttc ggcgatccgg agaatccgac ggttctgaag tcgtcggcca aggaactgca
     2581 ggagggactg ccgctccgcc tcagttttcc ggacgggctg gtgttctggt cgagaaacga
     2641 tagcggggaa tcactcatgc ggcgaggtgg aatgcggcgc cggctcgggc ttgcggcggt
     2701 gctgctcttc ggggttgtcg gtccggcctg cgacggtatc cgggtgaccc gcctgtatca
     2761 ggagggggcg gccgtgaccg cggcgcctct ggcgacgcag gtgggggagg agattttccg
     2821 ccgggggggg aatgcgttcg acgcggcggt agcgatcggg ttcgctctcg cggtcgtcca
     2881 cccacaggcg gggaatctcg gcggcggcgg ttttgccgtg atccggtcgg cgcaggcgga
     2941 tacggtgcgg acgcttgatt ttcgcgagac ggcgccccgg gcggccaccg gcggcatgta
     3001 cctggccgac agcgtgacgg tgatcgcgga agcgtcgacc gtcggggcgc tcgccgcggg
     3061 cgtgcccggc acggtcgccg ggctctacgc gctgtggcag gcctacggct cgatgccctg
     3121 ggaagagctc gtgcggccgg cggccctg
//
LOCUS       DAOOKS010000165         3148 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_37488_length_3148_cov_2.500808, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000165 DAOOKS010000000
VERSION     DAOOKS010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37488_length_3148_cov_2.500808"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1360
                     /locus_tag="PK112_03525"
     CDS             <1..1360
                     /locus_tag="PK112_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11154.1"
                     /translation="GQTADTISVATVNDAIAESNEAMTFVISNSSQGAITGATAVGTI
                     IDDDTGTDNTPPAAPVLHTPTQGSVLRTTTPVLGVLNSDDRDGDVLTYDFEVYDAVPS
                     LVAGVGGVAEGAATTRWTVPVALQDGAAYTWRARSYDGEEYSPWMSTASFSIDMSSGE
                     NNLPTLPVHASPSAGDTLVSAPIVLRVDNAIDPDGDDVTYDFFLYADAGLSELVEEHL
                     GVPETPQQTAVVLDLTPVDGSTYWWRVRASDGIGATAPTEATSFFYYSLYTGGEDEVP
                     EPVSPGSGATVYTTRPVLKARNAALAGTPAYFFQVASDETFTGIVSASDPVAEDPSGV
                     TEWQVADDLEVGETYYWRVRADNEAYSAPAAFQVGFEVVAYPNPVHFQQGEVVTITLP
                     DEPIDLLIQSISGETVLIEDGVSGRWTWDGRNASGNLVAIGVYSWFAKGGAYNGKIVV
                     MP"
     gene            1770..2765
                     /locus_tag="PK112_03530"
     CDS             1770..2765
                     /locus_tag="PK112_03530"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HPC11155.1"
                     /translation="MPEYKQIDRVIRILQRLYQQGHVTVRELYELFERQVPKRTLQRD
                     LVDISGAGVPLYNEKGRGREQIWCVDKHFLRFVPQTIGSQELLASFFLEQLALAARGT
                     QLEEDVRSLLDKARQLAGPGVFQSLQSGDLAHDMFGATFMGYVDYSEHSEKIDTLVKA
                     CAESRTCRFVYKAATREKPSEFEAEPYLMLYHKGAMYAVVYVPAHDNYIFLPVQRIRS
                     VEYTGERFTRQAGFSLDKLREGRFGIYGGPEYRPERVVLKFKPEIADVVAERVWHYSQ
                     QMTRNADGSLTLEMCTVISDELRAWIGSWLDRVEVLEPQGLVPQAGSGEHPEAAH"
     gene            complement(2824..>3148)
                     /locus_tag="PK112_03535"
     CDS             complement(2824..>3148)
                     /locus_tag="PK112_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11156.1"
                     /translation="GDSLAGTPAERDTATFSVFAADPFDTTGADVFVRTYICGDVDDN
                     GAGPNVADLTYLVTFLFRGGPEPPVLPAANLNGSTGPGNNIEVSDLTLLIAYLFRGGP
                     APTCG"
BASE COUNT          561 a          989 c         1077 g          521 t
ORIGIN      
        1 cggccagacg gcggacacca tctcggtcgc caccgtgaac gacgcgatcg cggagagcaa
       61 tgaggccatg acgttcgtga tctccaattc ctcgcagggg gcgatcacgg gcgcgaccgc
      121 ggtcgggacg atcatcgacg acgacaccgg cacggacaac acgccgccgg cggcgcccgt
      181 cctccacaca cccacccagg ggtcggtgct gcggacgacc acgcccgtgc tgggcgtgct
      241 caacagcgat gaccgggacg gcgacgtgct cacctatgat ttcgaggtgt acgacgccgt
      301 acccagcctg gtggccgggg tcggcggggt ggcggaggga gcggcgacca cgcggtggac
      361 ggtcccggtg gcgctccagg acggagcggc ctacacgtgg cgggcccgga gttacgacgg
      421 cgaggagtat tcgccgtgga tgagcaccgc ctcgttcagc atcgatatga gcagtgggga
      481 gaacaatctc cccacgctcc ccgtccacgc ctcgccgagc gccggcgaca cactcgtgtc
      541 ggcgcccatc gtcctgcggg tcgacaacgc gatcgacccg gacggcgacg acgtgacata
      601 tgattttttc ctctacgccg atgccggttt gagtgagctc gtcgaagagc acctcggcgt
      661 cccggagacg ccgcagcaga ccgccgtcgt gctcgatctg accccggtcg acggatcgac
      721 gtactggtgg cgggtccgcg cgagcgacgg catcggcgcg acggcgccga ccgaggcgac
      781 ctcgttcttc tactacagcc tctacacggg cggcgaggac gaagtccccg aaccggtcag
      841 tccgggaagc ggcgcgaccg tctacaccac tcgcccggtc ctgaaggccc gtaacgccgc
      901 gctcgccggc acgcctgcgt atttctttca agtggcgtcc gacgagacct tcaccggcat
      961 cgtctccgcc tcggatccgg tggccgaaga tccttcgggg gtgaccgagt ggcaggtggc
     1021 ggatgatctt gaggtcgggg agacctacta ctggcgggtg cgcgccgaca acgaagccta
     1081 cagcgcgccg gcggccttcc aggttggatt cgaggtggtc gcgtacccca acccggtgca
     1141 cttccagcag ggagaggttg tcacgatcac gctccccgat gagccgatcg atctcttgat
     1201 ccagagcata tcgggcgaga ccgtgctgat cgaagacggc gtctccggcc ggtggacctg
     1261 ggacggccgc aacgcctcgg gcaatctcgt ggcgatcggc gtgtattcgt ggttcgccaa
     1321 ggggggcgcg tacaacggca agatcgtggt catgccatag ctcgttggcc ggccctgata
     1381 ggaccagggc cgagggaccc cgaagagagc acgctcctcg tatccccatt ccggtaagac
     1441 acgaactcga ataatccggg gcggcgcggg cagggatagt ccggcgctcg atgccggttt
     1501 cattggcgcc gtccggagcc gccgaaaatg gcggaatgca gccactttcg caattcgcgg
     1561 tcggtcgctg cctccccgat cagcgcctcg accgctcaga cccccgcttg ccgcaccgca
     1621 cccccgcgat gacccccgat cacggggtgc gaactgcggc ccgcacgacg tctacccata
     1681 attagtgttg ccatgcgtca ttcggtgact catactctgt ttaatttcgg gaaaacatga
     1741 gcacgagagc tcgctaatgg agggtttaga tgcctgagta caagcagatc gaccgggtca
     1801 tccgcattct tcaaaggctt taccagcaag ggcacgtcac cgtcagagaa ctctacgagc
     1861 tgtttgagcg ccaggtcccg aagcggacgc tgcagcgcga cctcgttgat atttcggggg
     1921 cgggggttcc gctgtacaac gaaaaggggc ggggccgcga gcagatctgg tgtgtcgaca
     1981 agcactttct gcgttttgtt ccgcagacga tcggcagtca ggaactgctg gcgtcgtttt
     2041 tcctggagca actggctctc gcagcgcggg ggacacagct ggaagaggat gtccgctcgc
     2101 tcctggacaa ggcgcggcaa ttggccgggc cgggagtctt tcagtccctc cagagcgggg
     2161 atctggcgca cgacatgttc ggggcgacgt tcatgggtta tgtcgactac agcgagcact
     2221 ccgagaagat cgacacgctc gtcaaggcct gcgccgaatc gcgcacctgc cgatttgtct
     2281 acaaggcggc gacgcgggag aaaccctccg aattcgaggc cgaaccgtat ctgatgctct
     2341 accacaaggg ggcgatgtac gcggtggtgt acgtgccggc ccacgacaac tacatcttcc
     2401 tgccggtgca gcggattcgc tcggtcgaat acacggggga gaggttcacg cgccaggccg
     2461 ggttcagcct ggacaaactg cgcgaggggc gattcggcat ctacggcgga cccgagtacc
     2521 ggccggagcg ggtggtgctg aagttcaagc cggagatcgc cgacgtggtc gccgagcgcg
     2581 tgtggcacta tagccagcag atgacgcgca acgccgacgg ttcgctgacg ctggaaatgt
     2641 gcacggttat ctccgacgaa cttcgggcct ggatcggcag ctggctcgac cgcgtggaag
     2701 tgctggagcc gcagggtctg gtgccgcagg ccggctccgg ggagcatccg gaggcggcgc
     2761 actgaggccg cggcgggggg cacgctgccg ggtgttcgat ccgggcaggg atccgcgggt
     2821 tgttcacccg caggtcgggg ccgggccgcc tcggaacagg taggcgatca gcagggtcag
     2881 gtcggacacc tcgatgttgt tgccggggcc ggtgctgccg ttcaggttcg ccgccggcag
     2941 cacgggcggt tcgggcccgc cgcggaacag aaacgtgacc agataagtca ggtcggcgac
     3001 attcggtccc gcgccgttgt cgtcgacgtc gccacagatg taggtccgga cgaaaacgtc
     3061 ggcgccggtt gtgtcgaacg gatccgcggc gaagaccgag aacgtcgccg tatcgcgctc
     3121 ggcgggagtc cccgcgagcg aatcgccg
//
LOCUS       DAOOKS010000166         3143 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_37558_length_3143_cov_3.734780, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000166 DAOOKS010000000
VERSION     DAOOKS010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37558_length_3143_cov_3.734780"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1208
                     /gene="cysS"
                     /locus_tag="PK112_03540"
     CDS             <1..1208
                     /gene="cysS"
                     /locus_tag="PK112_03540"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HPC11157.1"
                     /translation="DDKTIRDSQKEGVSLKEFTTRYANAFFEDLDTLGIERAEVYPAA
                     TDHIPEMVEIIKELIAKGLAYEVGGNWYFRIERFPAYGRLAHIDRANLKAGARVAVDE
                     YEKDSVSDFALWKAWDEKDGDVYWETELGKGRPGWHIECSAMSMKYLGRHFDIHSGGV
                     DNIFPHHENEIAQSEGATGEPFVNYWLHAEHLIVEGRRMAKSFGNYYLLRDLLARGHS
                     GAQTRYVLISTHYRQQLNFTFQGLEAAKSALERYNDFIANLEDYAGGAASGGAAEAIT
                     ARMLAGFEEALDDDLNISGALAAVFDFVRDINRLRAEGTLAAEERDRALAAIRRVDTV
                     LNFTYERGTDEAVGPEIMALVEKRTAARAGRDFAAADRIRDELLARGYVLEDTPQGPK
                     VKRKQLGPR"
     gene            1257..1329
                     /locus_tag="PK112_03545"
     tRNA            1257..1329
                     /locus_tag="PK112_03545"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1290..1292,aa:Thr,seq:tgt)
     gene            1445..2389
                     /locus_tag="PK112_03550"
     CDS             1445..2389
                     /locus_tag="PK112_03550"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPC11158.1"
                     /translation="MNVIDSQDLTKVYSGGLRQRGIVALEAVNISVAEGEIFGLLGPN
                     GAGKTTFLKVVLGITRLTSGNVIVSGHKPTDARSRQRLGYLPENHRFPSHLTGLGLMI
                     ATGRLFRMSAAETRSRAANLLHLVGMDRWGDTKLKKYSKGMQQRIGIAQALVSDPDIL
                     LLDEPTDGVDPVGKVEIREVLRTIRAEGKSIVLNSHLLSEVESVADRVAILNRGRLVK
                     TATVEELTSRRSQFEIEAAIGNLLFSLPEGMGKIIKLSARGMIVELESEEDINWVIDE
                     LRIKKIPIRMIRPMRITLEQSFIETITTPEPPSTEAGI"
     gene            2386..3141
                     /locus_tag="PK112_03555"
     CDS             2386..3141
                     /locus_tag="PK112_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11159.1"
                     /translation="MRGLLQDTFKEMVERKLIWIYVAVTVIAVLMTIASHSMKIEINM
                     NSGSEDPIASLAQKVAVGAMDTYFSFLVFLTVMGTAGLIPSMLVRGRAEYYLSKPVSR
                     AALLLYKLFSIWAVYGATIVAGGLVTFLAFYAAWGFADVRLLYIFALNMAALFVWLSV
                     TAFSGILTGSFAVSIMMAFVMWIAQVLLQYHEAVQNVIDSRFVTGIVSGLYYVLPKTS
                     QLGEVTQSLALGRPVESWLPLWSRLVRTAPCRR"
BASE COUNT          586 a          972 c         1062 g          523 t
ORIGIN      
        1 tcgacgacaa gacgatccgg gactcgcaga aggaaggcgt atcgcttaag gagttcacga
       61 cccgctacgc caacgcgttc ttcgaggacc tcgacacgct cggcatcgag cgggcggagg
      121 tctacccggc ggcgactgac cacattcccg agatggtgga gatcatcaag gaactcatcg
      181 ccaaggggct ggcgtacgag gtcggcggca actggtactt tcgcatcgag cgcttcccgg
      241 cctacggccg gctggcgcac atcgaccggg ccaatctgaa agccggggcg cgggtggcgg
      301 tcgatgagta cgagaaagac tcggtctcgg acttcgctct gtggaaggcg tgggacgaga
      361 aggacggcga cgtgtactgg gagacggagc tcggcaaggg gcgtcccggc tggcacatcg
      421 agtgttcggc gatgtcgatg aagtacctcg ggcgccactt cgacatccac agcggcgggg
      481 tcgacaacat cttcccgcac cacgagaacg aaatcgccca gtcggagggg gccaccggcg
      541 agcccttcgt caactactgg ctccacgccg aacacctcat agtcgagggg cggagaatgg
      601 cgaagtcgtt cggcaattac tacctgctgc gcgatctgct ggcccggggg cacagcggcg
      661 cccagacgcg ctacgtgctc atctcgaccc actaccgcca gcagctcaac tttacgttcc
      721 aggggctcga ggcggccaag agcgcgctgg agcgctacaa cgacttcatc gccaatctcg
      781 aggactacgc cgggggcgcg gcctccggcg gcgcggccga ggcgatcacc gcccgcatgc
      841 tggccgggtt tgaggaggcg ctggacgacg acctgaacat ctcgggcgcg ctggcggccg
      901 tgttcgattt cgtccgcgac atcaaccggc tgcgggccga gggaacgctc gcggccgagg
      961 agcgggacag ggcgctggcg gcgatccggc gggtcgatac ggtgctgaat ttcacctacg
     1021 agcggggcac ggacgaggcc gtggggccgg agatcatggc tctggttgaa aagcggacgg
     1081 cggcccgcgc cgggcgcgat ttcgccgcgg ccgaccgcat ccgcgacgaa ctgctcgccc
     1141 gcggctacgt tctggaggat acgccgcagg ggcccaaagt gaagcggaag cagctgggac
     1201 cccggtaaaa ccttgacact ttcggcccgc gggcgcgtac attaacggag ttgcacgccg
     1261 gtgtagctca gctggtagag caactgattt gtaatcagta ggtcgggggt tcgaatccct
     1321 tcaccggctt tgattgcggc agggtgcggg cggcgaaggc aggacccggg gggagcggcg
     1381 gacaactgcg cgccggcggc cgggaacccc gggtttgcgg aaaaccaaat cgggcggaag
     1441 atctatgaac gttatcgaca gtcaggatct gaccaaggtc tacagcggcg gactccgcca
     1501 gcgcgggatt gtggccctcg aggcggtgaa catctcggtg gccgaagggg agatcttcgg
     1561 cctcctcggt ccgaacgggg cgggcaagac gacgtttctc aaggtcgtcc tggggatcac
     1621 ccggctcacc tcgggcaacg tcatcgtcag cgggcacaag cccaccgacg cgcggtcgcg
     1681 gcagcgcctg gggtacctgc ccgagaacca tcgctttccc agccacctga ccggcctcgg
     1741 cctgatgatc gcgaccgggc ggctgttcag gatgagcgcc gccgagaccc ggtcgcgcgc
     1801 cgccaacctc ctccacctgg tgggcatgga ccggtggggg gacaccaagc tcaagaagta
     1861 ctcgaaaggg atgcaacagc gcatcggcat cgcccaggcg ctcgtctccg acccggatat
     1921 cctgctcctg gacgagccga ccgacggcgt cgacccggtc ggcaaggtcg agatccgcga
     1981 ggtgctccgg acgatccggg ccgagggcaa atcgatcgtg ctcaattccc accttctgtc
     2041 ggaggtggag tcggtggccg accgggtggc gatcctcaac cgcgggcggc tcgtcaagac
     2101 ggcgaccgtc gaggaactca ccagccggcg gagccagttt gaaatcgagg ccgcgatcgg
     2161 caacctcctg ttcagtctcc cggagggcat gggcaagatc atcaaactct cggcccgcgg
     2221 gatgatcgtc gaactggaaa gcgaagagga catcaactgg gtgatcgacg agctgcgcat
     2281 caagaaaatt ccgatccgga tgatccgccc catgcgcatc acgctcgagc aatcgttcat
     2341 cgagaccatc acgacccccg aaccgccatc cacggaggcc ggcatatgag agggctgctc
     2401 caggatacgt tcaaggaaat ggtcgagcgc aagctgatct ggatttacgt ggcggtgacg
     2461 gtgatcgcgg tgctgatgac gatcgcctcc cactcgatga agatcgaaat caacatgaat
     2521 tccggaagcg aggatcccat cgcgagcctg gcccagaaag tggccgtggg ggcgatggac
     2581 acctactttt cgtttctcgt tttcctgacc gtcatgggca cggccggcct gattccgtcg
     2641 atgctggtcc gggggcgggc ggagtactac ctgtcgaagc cggtatcgcg cgccgcgctg
     2701 ctcctgtaca agctgttcag catctgggcg gtgtacggcg cgacgatcgt ggccggcggg
     2761 ctggtgactt tcctggcatt ctacgcggcc tgggggtttg ccgacgtccg gctgctctac
     2821 atcttcgcgc tcaacatggc cgccctcttc gtctggctga gcgtgaccgc tttctccggc
     2881 atcctgaccg gatcgttcgc ggtgagcatc atgatggcgt tcgtgatgtg gatcgcccag
     2941 gtcctcctgc agtaccacga ggccgtgcaa aatgtcatcg acagccggtt cgtcaccggg
     3001 atcgtgagcg ggctgtacta cgttctgccg aaaacgagcc agctcggcga ggtcacccaa
     3061 agcctcgccc tcgggcggcc ggtggaatcc tggttgccgc tctggtcgag gttggtgcga
     3121 accgccccct gcaggaggta atc
//
LOCUS       DAOOKS010000167         3136 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_37658_length_3136_cov_3.319377, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000167 DAOOKS010000000
VERSION     DAOOKS010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37658_length_3136_cov_3.319377"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1207
                     /locus_tag="PK112_03560"
     CDS             <1..1207
                     /locus_tag="PK112_03560"
                     /inference="COORDINATES: protein motif:HMM:NF015647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HPC11160.1"
                     /translation="CSRDFLEGMSLRRLISRARLTVLGLNSGTSADGLDLAVVQVGRG
                     REKILMRGGGAVRFPRPLRAAILATADSRTVEPAELVRLDNYLGQVMGRAAAGFLKTL
                     SRRGIAVDLIGSHGQTVRHVPEPVRMGGMAVRGTLQIGSPDQVAAATGCVTVGDFRQA
                     EVALGGEGAPITTGAMHWLFASPREPRLIVNIGGIANYFYLPPTASGEAPTARDCGPG
                     NSLSDALMARSFGRQFDRDGRMARRGRIDPRLLAALMESLGFARRRRSTGRELFGADL
                     ADEVVRLGRRRRLRPEDLLATAGELTALGIARALHRLPASDPPPRRVYLMGGGRHNQF
                     MVERLGEHLPGWSVEAIDQLGVDGDLVEAAAYAVMAAACVRGEPLSGARTFGVARAIA
                     GRIVQPPAG"
     gene            1211..2494
                     /locus_tag="PK112_03565"
     CDS             1211..2494
                     /locus_tag="PK112_03565"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HPC11161.1"
                     /translation="MIDRDNLRIKRLIRLTARVGTALCLMVLVWSCGRVPGLKDEEPG
                     SFIRVPFVRVLLGEDRPEATVSADHAFAIECLTAGEQHVFYSSRPVRIQSLGREVRVL
                     TRDGQIIQDRAGEVNIIPRGTGNRTMVDGRPYRGIIHLLPYANNLRIINIVYVEDYLR
                     GVVPPELGERADGEIEAVKAQAVAARTYAMSHLEQYGGEPYDLKASVYDQVYEGYGVE
                     NRLVNRAVDATAGVVATFEGKMINAYYHSTCGGMTDDIAHIWDRPSIPYLRAVADSGA
                     CGWSKYYRWREVYTEGQLRGRVEQYLSGDRGRDLRVGEIRNVAVAERTPGGRVSTLVI
                     ETEMDTYRFHKDRIRWVIGRTSNPDLILPSDRFDVILERTGEGRISRINFEGSGYGHG
                     VGMCQCGAIGMSRTGYTYEQILKRYYAGCELKQLY"
     gene            2494..>3136
                     /locus_tag="PK112_03570"
     CDS             2494..>3136
                     /locus_tag="PK112_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11162.1"
                     /translation="MTSGLDQRARPRRTRWLVTMLLLALPAAGAARPYFIGARGELAT
                     LTGGHAPHFYVRGAYGLEAGLDLSPRWSLEASLSRYDLYNDTTIAGSVTLNGDREAAT
                     ARFSGTRLGVDAVATLFAPASWLSVRGGPGGGLLIWKMRDPDGDTVLKAVGGRGETID
                     FASTEIFVSGRLGIEVQLAAHLSTAITAHVDYLTGAGTDFADAVNARRDRWLGG"
BASE COUNT          480 a          931 c         1230 g          495 t
ORIGIN      
        1 gtgcagccgt gactttcttg aagggatgtc gctgcggcgc ctcatatcgc gagcgcggct
       61 gaccgtgctc ggattgaact cgggcacctc ggccgacgga ctggatctgg cggtggtgca
      121 ggtgggccgc ggccgggaga agatcctcat gcggggcggc ggggccgtgc ggttcccgcg
      181 gccgctgcgg gccgcgatcc tggcgaccgc cgacagccgc acggtggaac cggccgagct
      241 ggttcgcttg gacaactacc tgggacaggt catggggaga gcggccgccg ggtttctgaa
      301 gacgctttcg cgccggggga tcgcggtcga tctgatcggt tcgcacgggc agacggtgcg
      361 gcatgtcccg gagccggtgc gcatgggggg aatggcggtg cgcgggacgc tgcagatcgg
      421 atcgcccgac caggtggcgg cggcgaccgg gtgcgtgacg gtgggggact tccgccaggc
      481 ggaggtcgcg ctcggcggcg aaggggcgcc gatcaccacg ggggcgatgc actggctctt
      541 cgcatcgccg cgcgagccgc gcctgatcgt gaacatcggg gggatagcca attatttcta
      601 tctcccgccg acggcgtcgg gcgaggcgcc cacggcgcgc gactgcggtc cgggcaacag
      661 cctctccgat gcgctcatgg cgcggtcgtt cgggcggcag ttcgaccggg acgggcgcat
      721 ggccaggcgg gggcggatcg acccgcgcct gctggcggcg ctgatggaaa gtctggggtt
      781 tgcacggcgc cgccggtcga ccgggagaga gctcttcggg gcggatctcg ccgatgaggt
      841 ggtccggctg gggcggcggc gccggcttcg gccggaggat ctgctggcca cggcggggga
      901 gttgacggcc ctcgggatcg cccgcgccct gcaccgcctt cccgcctccg atccgccgcc
      961 gcggcgggtg tatctcatgg gcgggggacg gcacaaccag ttcatggtcg aacgcctggg
     1021 ggagcatctc cccgggtggt cagttgaggc gatagatcag ttgggagtgg acggcgactt
     1081 ggtggaggcg gcggcctatg cggtgatggc ggcggcgtgc gtacgcgggg agcctctctc
     1141 cggagcgagg acattcggtg tcgctcgggc aatcgcgggg cgcatcgtgc agccgccggc
     1201 ggggtgacga atgattgacc gggacaacct caggatcaaa agactcatcc gcctcacggc
     1261 ccgcgtcggg accgctctct gcctcatggt gctcgtgtgg agctgcggac gggtgccggg
     1321 gctcaaggat gaggagccgg gctcatttat ccgcgttccc tttgtccggg tgctgctggg
     1381 ggaggaccgc ccggaggcaa cggtttcggc ggaccacgct ttcgctatcg agtgcctcac
     1441 ggccggggag cagcatgtct tttactcctc gcgtccggtg aggatacaga gcctgggccg
     1501 cgaggtgcgg gtgctgacgc gggacgggca gataatccag gaccgcgcgg gggaagtgaa
     1561 catcattccg cgcgggacgg ggaatcggac gatggtcgac ggtcggccgt accggggaat
     1621 catccaccta ctcccctacg ccaacaacct gcgcatcatc aacatcgtgt acgtcgagga
     1681 ctacctccgg ggggtggtgc cgccggagct gggagagcgg gcggacgggg agatcgaggc
     1741 ggtgaaagcg caggcggtgg cggcccggac ctacgccatg tcccacctcg agcagtacgg
     1801 cggggagccg tacgatctga aagccagcgt ctatgaccag gtgtacgagg gctacggcgt
     1861 ggagaaccgg ctggtgaacc gggcggtcga cgcgacggcg ggtgtcgtgg ccactttcga
     1921 gggcaagatg atcaacgcct actaccattc gacgtgcggc ggaatgaccg acgacatcgc
     1981 ccacatctgg gaccgcccga gcattcccta cctgcgggcg gtggccgaca gcggggcgtg
     2041 cgggtggtcg aagtactacc ggtggcgcga ggtgtacacg gaggggcaac tgcgcggccg
     2101 ggtggagcag tacctctcgg gggaccgcgg ccgggacctt cgggtcggag aaatccggaa
     2161 cgtggcggtg gcggagcgga caccgggggg gcgggtctcg accctggtga tcgagacgga
     2221 gatggacacg taccgtttcc acaaggaccg gatccgctgg gtgatcgggc gcacctcgaa
     2281 tcccgatttg atcttgccgt cggatcgctt cgacgttata ctcgagcgga ccggggaggg
     2341 ccgaatcagc cggatcaatt tcgagggcag cggctacggc catggggtcg gcatgtgcca
     2401 atgcggggcg atcggcatgt cccgcacagg gtatacgtac gaacagattc tcaagcgcta
     2461 ctatgccggt tgtgaactca agcagttgta ctgatgacga gcggacttga ccagagggcg
     2521 cggccgcggc gaacgcggtg gctggtgacg atgcttttgc tggcgctgcc ggcagccggg
     2581 gcggcgcggc cgtatttcat cggggcccgg ggggagctgg cgacgctcac aggcggacac
     2641 gcgccgcact tctacgtgcg cggggcgtac ggcctcgagg cgggcctgga cctgtcgccg
     2701 cgctggtcgc tggaggcaag tctcagccgc tacgacctct acaacgacac gacgatcgcg
     2761 gggagcgtaa ccctgaacgg cgaccgggag gctgcgacag cgcggttctc gggcacgcgg
     2821 ctgggcgtgg atgcggtggc gaccctgttc gcgcccgcct cctggctgag cgtccggggc
     2881 ggccccggtg ggggcctgct catctggaag atgcgcgatc cggacggcga caccgttttg
     2941 aaggcggtcg gcgggcgggg cgagacgatc gatttcgcct cgaccgaaat cttcgtatcg
     3001 gggcggctgg ggatcgaagt gcagctggcg gcccatctgt cgacggcgat cacggcgcat
     3061 gtcgactacc tgaccggggc gggaacggat ttcgccgatg cggtgaacgc gcgccgggac
     3121 cgctggctgg ggggag
//
LOCUS       DAOOKS010000168         3129 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_37759_length_3129_cov_2.296357, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000168 DAOOKS010000000
VERSION     DAOOKS010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37759_length_3129_cov_2.296357"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="PK112_03575"
     CDS             complement(<1..628)
                     /locus_tag="PK112_03575"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HPC11163.1"
                     /translation="MGIRIGTSGFSFEDWRGSFYPETIDKGKMLDYYVERFSTVEINS
                     TYYRIPHPAVMRNLAKKAPEGFDFVVKLPQSVTHRRQDIEEDLRQFLQAVEPMEASGK
                     LSGLLAQFPYSFRFSPDSLDYLGALRAALAPRPLFTEFRHRSWVNRTMYDRLRAEGIG
                     YVCVDEPELPNLIKPDLFTTGPTAYLRLHGRNAEYWWEGGALRYDYRYS"
     gene            complement(877..1491)
                     /locus_tag="PK112_03580"
     CDS             complement(877..1491)
                     /locus_tag="PK112_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11164.1"
                     /translation="MDWGTAVAGALTLAILSFLYRDNPVYRMAESLLIGVAIGYFLVI
                     TWTNTLMDLLVRPLFGDGRLGLIAPLVFGLLMFGRFHPSTKSWSRLSMAVLIGSGAGV
                     AIPVMLEARTLKQMSATVMPLVGPTGMPNWSAIVVMVGVVTTLSYFYFSREHKGALGR
                     SAKIGVYFLMIFFGTTFGYTVMSRMSTFIGRVEFLLSDFLRLTS"
     gene            complement(1491..2306)
                     /locus_tag="PK112_03585"
     CDS             complement(1491..2306)
                     /locus_tag="PK112_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11165.1"
                     /translation="MKLESRHWIFLGLGAAVVAAMALQVNLQVPASGDTLGMHRFIEE
                     VPAGSTIIVSFDHEASSLPEIRPLALAVLRHAFSRNLKIVGTALLAEGTGVGYRLVQQ
                     TAREYDKRYGEDWVYLGFKPQYIAAILSMGESFAKTWPEDYLGRPYTDLPMLAEIETY
                     GQIAGVVSVADGSLTTHWIEYAASRYRVRVAAAVTAAMVTTYDPYLASGQLQAMVGGP
                     RGAAEYEQLIARGGGGERGMLAQTVSHLYVVALIVVGNLVFFATRRREKGGGG"
     gene            complement(2303..2929)
                     /locus_tag="PK112_03590"
     CDS             complement(2303..2929)
                     /locus_tag="PK112_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11166.1"
                     /translation="MGRRLPQFIVLVFGAVMFLQYFSDAPAARSINSAVLVDYWQIIF
                     AFTLLVGVVSYVRTATAGIGRAADWPYRAVSLAGVALMPVLAVLWGIRAESPFMWVFE
                     NVQVPMQATVFALLAFFVASASYRGFRARSAPAAVLLAAAFLTILTRSALGDWYPDWV
                     NRVAEWVRDVPSMAARRAILIGIGLGSLTTSLRVILGIERTWLGRGGR"
     gene            complement(2930..>3129)
                     /locus_tag="PK112_03595"
     CDS             complement(2930..>3129)
                     /locus_tag="PK112_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11167.1"
                     /translation="AFFIVACDYVLIGEELFAASGYLSEDPSIIASVRAQDILKVLIV
                     VLLIAAVVWATFAPDSTWWRL"
BASE COUNT          596 a         1108 c          967 g          458 t
ORIGIN      
        1 gcgagtagcg gtagtcatag cgcagcgctc ccccctccca ccagtactcg gcgttgcggc
       61 cgtggagccg caggtaggcg gtcggcccgg tcgtgaacag atccggcttg atcaggttgg
      121 gcagttccgg ttcgtccacg cagacgtagc cgatcccttc cgcccggagg cggtcataca
      181 tggtacggtt cacccaactc cggtggcgga actcggtgaa cagcggccgg ggggcgaggg
      241 cggcccggag cgcgcccagg tagtccaggc tgtccgggct gaaccggaac gaatagggaa
      301 actgggccag caggccggac agcttccccg acgcctccat cggctcgacc gcctgcagga
      361 actgccggag atcctcctcg atgtcctgcc gccggtgcgt gaccgactgc ggcagtttca
      421 ccacaaagtc gaatccctcg ggcgccttct tcgccagatt tctcatcacc gccgggtgcg
      481 ggatacggta gtaggtggag ttgatctcga cggtcgagaa ccgctccacg tagtagtcca
      541 gcattttccc cttgtcgatc gtctccggat agaagctgcc tctccaatcc tcgaagctga
      601 atcccgatgt gccgatgcgt attcccacgg ttccgtttcc gctcctttgg gccggctgcc
      661 aagtgtggta cgttgccgcc cgcggccggt tgcggcccgg ggcgaccggc ccgcgcgccg
      721 cttcagcggt cgccggaggg aggccggcgg tcggccggcc gccgctggaa gcgcacacgt
      781 ccgcactttg gacacaccca cttgcgctgc ttgtggaacg tccgtttcag ttccttcatc
      841 gtcgcctggc agcggggaca gcgcatccgg cccccgtcag ctggtcaggc ggaggaagtc
      901 gctgaggagg aattccaccc gcccgataaa cgtcgacatc cgcgacatga ccgtgtagcc
      961 gaaggtggtc ccgaagaaga tcatcaggaa gtacaccccg atcttcgccg accgccccag
     1021 cgcccctttg tgttcccggc tgaaatagaa atacgagagg gtggtcacga ctccgaccat
     1081 gaccacgatc gccgaccagt tgggcatccc ggtcgggcca accagcggca tcaccgtggc
     1141 cgacatctgc ttgagcgtcc gggcctcgag catcacgggt atggccacgc ccgctccgga
     1201 gccgatcagc accgccatcg acagccgcga ccaggatttg gtgctcgggt ggaaccgccc
     1261 gaacatgagc agcccgaaca cgagcggcgc gatcaggccg agccgcccgt ccccgaacag
     1321 cggccgcacg agcaggtcca tgagcgtgtt ggtccacgtg atcacgagga agtacccgat
     1381 cgccaccccg atcaaaagcg actccgccat ccggtagacc ggattgtcgc ggtacaggaa
     1441 actcaggatc gccagcgtga gcgcgcccgc gaccgccgtg ccccaatcca tcagccgccc
     1501 cctcctttct cccgccgccg ggtcgcaaag aagacaaggt tcccgacgac gatgagcgcg
     1561 accacgtaga ggtgcgagac cgtctgggcc agcatccccc gctctccccc gccaccgcgc
     1621 gcgatgagct gttcgtactc ggccgccccg cgcggcccgc cgaccatggc ctgcaattgc
     1681 cccgaggcca ggtacggatc ataggtggtc accatcgcgg ccgtcaccgc cgccgccacc
     1741 cgcacgcggt agcggctggc cgcgtactcg atccagtggg tcgtcagcga cccgtcggcc
     1801 accgacacga ctccggcgat ctgaccatag gtctcgatct ccgccagcat cggaaggtcc
     1861 gtatacggcc gcccgaggta atcctccggc caggtcttcg cgaacgactc ccccatcgag
     1921 agaatcgccg cgatatactg cggcttgaac ccgaggtaca cccagtcctc gccgtagcgc
     1981 ttgtcatact cccgggccgt ctgctgcacc aggcggtagc cgacccccgt cccctcggcc
     2041 agtagggcgg tgccgacaat cttcaggttg cgcgagaagg catgccggag taccgccagc
     2101 gccaggggcc ggatttccgg gagcgaggag gcctcgtggt cgaacgacac gatgatcgtc
     2161 gacccggccg gcacctcctc gatgaaccgg tgcatcccga gcgtatcgcc ggaggccggc
     2221 acctgcagat tgacctgcag cgccatggcc gcgaccacgg ccgccccgag accgaggaag
     2281 atccagtggc gcgattccag cttcatcttc cgcctctccc cagccaggtg cgttcgatcc
     2341 ccagaatcac gcgcagcgag gtggtgagcg acccgaggcc gatgccgatg agaatcgccc
     2401 gccgcgccgc catcgagggt acatcgcgca cccactcagc gacccggttc acccagtcgg
     2461 ggtaccaatc cccgagcgcc gagcgggtga gaatcgtcag gaacgcggcc gccaggagca
     2521 cagccgccgg cgccgatcgc gcccggaacc cgcggtacga ggccgaggcg acgaaaaacg
     2581 caaggagcgc aaacacagtc gcctgcatag gcacctggac attctcaaag acccacatga
     2641 agggggactc ggcccggatt ccccacaaaa ccgccagcac cggcatgagc gcgacgcccg
     2701 ccagcgacac cgcgcggtag ggccaatcgg ccgcgcgtcc aatcccggcc gtggccgtcc
     2761 gcacataact caccaccccc accagcagcg tgaaggcgaa aataatctgc cagtaatcga
     2821 cgagcaccgc ggagttgatg ctccgggccg ccggcgcatc ggagaagtac tggaggaaca
     2881 tgactgcgcc gaacaccagc acgataaact ggggaagccg ccgtcccatc tacagcctcc
     2941 accacgtgct gtccggggcg aaggtcgccc agaccaccgc cgcaatgagc agcactacga
     3001 tgagcacctt cagaatgtcc tgagcccgga cggaggcgat gatggaggga tcctccgaga
     3061 ggtagcccga ggccgcgaac agctcctcgc cgatcagaac gtagtcgcag gccacgatga
     3121 agaaggcca
//
LOCUS       DAOOKS010000169         3126 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_37807_length_3126_cov_3.267340, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000169 DAOOKS010000000
VERSION     DAOOKS010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37807_length_3126_cov_3.267340"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..984)
                     /locus_tag="PK112_03600"
     CDS             complement(<1..984)
                     /locus_tag="PK112_03600"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HPC11168.1"
                     /translation="MNLSLLDWAIVIVSLGLMIASVEGGRRVMRSVADFLSAGRSAGR
                     YVLCVASGAAGIGAITVVGNLEMNLIAGFSLTWWGMTTALVVLLITVSGWVIYRFRET
                     RSLTLAQYFEARYSREFRIFTGFIAFLSGIVNFGIFPAVEARFFIYFCGLPESVSLLG
                     FEVPLFPLMMLFLLGVSLYFVLGGGQVSVIYADFFQGVLVYGVFIALVVYFFVSFDWG
                     HIEQALAAAPRDASLINPFKTSQVEDFNFWYFLVGIIGVIYGTMSWQGTQAYNSSARS
                     AHEAKMASVLSSLRNLPMSIMFLFVPIVAYTVLNHPDFASIAAAVSDTLSGI"
     gene            complement(1000..2172)
                     /locus_tag="PK112_03605"
     CDS             complement(1000..2172)
                     /locus_tag="PK112_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014068230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HPC11169.1"
                     /translation="MKCDRCEGVPAARIALFVLLLAVVSGCRPEPGRGASAGSSGDTL
                     PVPAIPYAPRGYVCCRTAEGLTVDGRLEEAAWRQARWTELFTDIEGDRKPAPRFRTRA
                     KMLWDSTCFYIGAELAEPHVWATLTRRDAVIYHDNDFEVFIDPDADTHEYYELEVNAL
                     NTVWDLLLLKPYRDGGPAVNAWDIAGLQTAVAVEGTLNDPSDVDSGWRVEIAIPWAVL
                     AECAHRPSPPREGDRWKVNFSRVEWQVEARDGVYAKVTDPATGRPLPEDNWVWSPQGL
                     IAMHYPEMWGLVQFSAAPPGSPEAALVADPADSARWALRQVYYAERNYQAAHGRFTAA
                     HSDLALETAAPRGFTWPPALRATEDRFEAEVVRRDGGGALRITEDGRTAPTGQAGG"
     gene            complement(2162..>3126)
                     /locus_tag="PK112_03610"
     CDS             complement(2162..>3126)
                     /locus_tag="PK112_03610"
                     /inference="COORDINATES: protein motif:HMM:NF015971.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HPC11170.1"
                     /translation="MKRYSGNPLISRTDIPCIKPSLVDVSPVFNPGAVRWRGQVVLIL
                     RVQNRGRETFLLTAFSDNGIQFRIDRRPITFSGLERFPHRLHHIYDPRLTRIGDVCYV
                     TLAMDTDAGCRVGLARTTDFRAFEFLGEILHDEARNAVIFPERIGGAYAMLYRPNRTR
                     LEGGVASGDAIEWAVSENLQDWSPRGPVMAGRPHYWDELIGSGPPPLRTRHGWLHVYH
                     GVATHFAASNIYQAGVVLLAADHPGRVIARSRANILEPRELYELTGQVPNVVFPTGLI
                     PARDGGDGAVEDDAELLLYYGAADTCVGLATATVGELLEACDEV"
BASE COUNT          555 a         1090 c         1037 g          444 t
ORIGIN      
        1 gatccccgac agggtgtcgg acaccgccgc tgcaatcgac gcaaaatcgg ggtggttgag
       61 aaccgtgtag gcgacgatcg gcacgaacag gaacatgatc gacatcggaa ggttgcgcag
      121 cgacgagagc acgcttgcca tcttcgcctc gtgcgcgctc cgggccgacg aattgtaggc
      181 ctgcgtcccc tgccacgaca tcgtcccgta gatcacgccg atgatgccca cgaggaagta
      241 ccagaaattg aaatcttcca cctggctcgt cttgaacggg ttgatcagcg aggcatcccg
      301 cggcgccgcc gccagcgcct gctcgatgtg cccccagtcg aacgagacga agaagtacac
      361 aacgagggcg atgaacacgc cgtagacgag cacgccctgg aagaagtcgg cgtagatgac
      421 cgatacttgt ccgccgccga ggacgaagta gagcgagacg cccagcagga agagcatcat
      481 gagcgggaag agggggacct cgaacccgag cagggagacc gattccggca gaccgcagaa
      541 gtagatgaag aaccgggctt cgacggccgg gaagattccg aaattgacga tgccggagag
      601 gaaggcgatg aagccggtga agatgcggaa ctcccggctg tagcgggcct cgaaatactg
      661 cgccagagtc agcgagcgcg tctcgcgaaa ccggtagatc acccatcccg acacggtgat
      721 caagagtacc acgagcgccg tcgtcatccc ccaccaggtg agcgagaagc cggcgatgag
      781 gttcatctcg aggttgccga cgaccgtgat tgcgccgatg ccggcggcgc cgctggccac
      841 gcacaggaca taccgcccgg cggagcgccc ggccgagagg aaatcggcca cgctccgcat
      901 cacccgccgc ccgccctcca ccgaggcgat catgagcccg agcgacacga tgacgatggc
      961 ccagtcgaga aggctgagat tcatgggtgt ccgccttgcc tagccgcccg cctgcccggt
     1021 cggcgccgtg cggccgtctt ctgtaatccg gagcgccccg ccgccgtcgc ggcggacaac
     1081 ctccgcttca aaacgatctt ccgttgcccg gagcgccggc ggccaggtga aaccgcgcgg
     1141 tgcggcggtc tcgagcgcga gatccgagtg ggccgcggtg aagcggccgt gcgcggcctg
     1201 gtagttgcgc tcggcgtagt acacctggcg gagcgcccag cgggcggaat ccgccgggtc
     1261 ggcgaccagg gccgcctcgg gcgacccggg cggcgccgcc gaaaactgca ccagccccca
     1321 catctccggg tagtgcatcg cgatcagtcc ctgcggcgac cagacccagt tgtcctccgg
     1381 cagcggccgg ccggtcgccg gatcggtcac cttcgcatac acgccgtcgc gcgcctctac
     1441 ctgccattcg acgcgggaga aattgacttt ccaccggtcc ccctcgcgcg gcgggctcgg
     1501 ccggtgcgcg cactccgcca ggaccgccca gggaatggcg atctccaccc gccaccccga
     1561 gtcgacgtcc gacggatcgt tgagcgtccc ctccaccgcc accgccgtct gcaaacccgc
     1621 gatgtcccag gcgttgaccg ccggtccccc gtcccggtag ggcttcagca ggagcaggtc
     1681 ccacacggtg ttgagcgcgt tcacctcgag ttcgtagtac tcgtgcgtgt ccgcgtcggg
     1741 atcgatgaac acctcgaaat cattgtcgtg gtagatgacc gcgtcgcgcc gggtgagggt
     1801 cgcccacacg tgcggctcgg ccagttcggc cccgatgtag aagcaggttg agtcccacag
     1861 cattttggcg cgcgtgcgga aacgcggagc cggtttccgg tccccctcga tatccgtgaa
     1921 cagctcggtc caccgggcct gccgccacgc ggcctcctcc aggcggccgt cgaccgtgag
     1981 gccctcggcc gtccggcagc acacgtagcc gcgcggtgcg taggggatcg ccgggaccgg
     2041 cagagtgtcg cccgacgatc cggccgaggc ccctcgcccc ggctccggtc ggcatccgga
     2101 gacgacggcc agcagcagca caaacagggc gatccgggcc gccgggacgc cttcgcaccg
     2161 atcacacttc atcgcacgcc tcgagcaact cgccgacagt cgcggtcgcc agtcccacgc
     2221 acgtgtcggc cgccccgtag tagagcagga gctcggcgtc gtcctcgacc gcgccgtcgc
     2281 cgccgtcgcg ggccgggatc agcccggtcg gaaagaccac gttcggcacc tggccggtca
     2341 gctcgtagag ttcccgcggt tccaggatat tggcgcggct gcgcgcgatc acccggccgg
     2401 ggtggtcggc ggccagcagt acgaccccgg cctggtagat gttgctcgcc gcgaagtggg
     2461 tggccacccc gtggtacacg tggagccagc cgtggcgggt ccggagcggg ggcggccccg
     2521 acccgatcaa ctcgtcccag tagtgcggcc gcccggccat caccggcccg cggggcgacc
     2581 agtcctgcag attctccgac accgcccact cgatcgcatc gccgctggcc accccgccct
     2641 ccagccgcgt ccggttcggc cgatacagca tcgcgtacgc cccgccgatc cgctccggga
     2701 agatcaccgc gttgcgcgcc tcatcatgca ggatctcgcc gaggaattcg aacgcgcgaa
     2761 aatccgtggt ccgggccagg ccgacccggc agccggcatc ggtatccatc gccagcgtga
     2821 cataacacac gtcgccgatc cgggtcagcc gcgggtcgta gatatggtgg aggcggtgcg
     2881 ggaagcgctc gaggccggag aaggtgatcg ggcggcggtc gatccggaac tgaatgccgt
     2941 tgtcgctgaa cgccgtcagg aggaaagtct cgcggccccg gttctggacc cggaggatca
     3001 gcaccacctg gccgcgccac cgcaccgcgc cgggattgaa caccggcgac acatccacca
     3061 gcgacggttt gatgcaggga atatcagtgc ggctgatcag cgggttcccg gagtagcgct
     3121 tcatcg
//
LOCUS       DAOOKS010000170         3113 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_38020_length_3113_cov_3.411053, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000170 DAOOKS010000000
VERSION     DAOOKS010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38020_length_3113_cov_3.411053"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1842
                     /gene="nuoL"
                     /locus_tag="PK112_03615"
     CDS             <1..1842
                     /gene="nuoL"
                     /locus_tag="PK112_03615"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HPC11171.1"
                     /translation="AFILSVLAFFELRALPPAMRMIEQTLFTWIPSGNFNVNIGFLLD
                     PLSAVMILVVTGVGFLIHVYATGYMAHDPGFGRFFAYLNLFMFAMLTLVLADNFLLMF
                     VGWEGVGLCSYLLIGFWYERQSAADAGKKAFLVNRIGDFGFLLGMFLIFWQVGSLHFP
                     TVSEAAPAVFTAGGGLITAVCLLLFLGATGKSAQIPLYVWLPDAMEGPTPVSALIHAA
                     TMVTAGVYMIARTNVLYSLASDALLVVAVIGAATAFVAATIGLAQNDIKRVLAYSTVS
                     QLGYMFLACGVASFTAGIFHLMTHAFFKALLFLGAGSVIHAMSDKQDMREMGGLRKHL
                     PVTYATMLVATLAIAGIPGFSGFFSKDEILWKSFSSAHGHPLFWVIGFATAGLTAFYM
                     FRLVFLTFFGKERMDQHTKDHLHEFPRSMTVPLVILAVLSVVGGYVGIPHVFGATNWF
                     ERWLEPVMAGGGGEAAPETVHASAGGYGTGMELALMGASVALVLLAIYGAHLFYRKRT
                     ELATRWRERLSGLHRLLVNKYYVDEIYLAVIVRPLVGLSMFLWKFVDVIVIDGLANGS
                     AAAYQGLSEGVRRLQTGRVRSYATLFVLGVVLLVAYFAMDWGWKIKF"
     gene            1824..>3113
                     /locus_tag="PK112_03620"
     CDS             1824..>3113
                     /locus_tag="PK112_03620"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HPC11172.1"
                     /translation="MENQILTYVTFFPLVGIVALLLAPKERHDTIKAVALIVAVITML
                     LSFFLWSMFDPMALGMQFEVDIPWITGLGIHYHLGIDGISLLLIALTTVLTVLAILAS
                     WNSITKGVKGYYLSMLLLQVGMIGVFVSLDLFLFYVFWEIMLVPMYFIIGLWGGPRRV
                     YAAIKFVLFTMFGSLLMLVGILWLLFAYHGYSGEYSFDMLKLVEMPIGYRAQLLLFGA
                     FALAFAIKVPLFPFHTWLPDAHVEAPTAGSVILAGVLLKMGTYGFVRICLPMFPDATV
                     AYLPYIAVLSVIAIIYGALVAMVQRDVKSLVAFSSVSHMGFIMLGIFALNLQGLQGAI
                     MQMVNHGVSTGALFLLVGMIYDRRHTRLIEEFGGLAKVMPAYSAIFMIVALSSIGLPF
                     TNGFVGEFLILLGAFDRSYVYGTLAASGVIFAAAYMLW"
BASE COUNT          433 a         1058 c          929 g          693 t
ORIGIN      
        1 gccttcatcc tctcggtgtt ggctttcttc gagctgcggg cgctgccgcc ggcgatgcgg
       61 atgatcgagc aaaccctctt cacctggatc ccctccggca atttcaacgt caacatcggc
      121 ttcctgctcg acccgctctc cgccgtgatg atcctcgtgg tgaccggcgt gggattcctc
      181 atccacgtct acgcgaccgg ctacatggcc cacgacccgg ggttcggacg gttcttcgcc
      241 tacctcaacc tgttcatgtt cgcgatgctc acgctcgtgc tcgccgacaa cttcctgctc
      301 atgttcgtcg gctgggaggg ggtcggcctc tgctcctacc tgcttatcgg tttctggtac
      361 gaacggcagt cggcggcgga cgcggggaag aaggcattcc tcgtcaaccg catcggcgac
      421 ttcggtttcc tcctggggat gttcctcatt ttctggcagg tcgggtcgct ccatttcccc
      481 accgtgagtg aggcggcgcc cgcggtgttc accgccggag gcggcctcat caccgccgtc
      541 tgcctcctgc tcttccttgg cgcgaccggg aaatcggcgc agatcccgct ctatgtctgg
      601 ctgcccgacg ccatggaggg cccgacgccg gtgtcggcgc tcattcacgc cgccacgatg
      661 gtgaccgcgg gcgtctacat gatcgcgcgg acgaatgtgc tgtacagcct cgcctccgac
      721 gccctgctcg tggtggcggt catcggcgcc gcgaccgcct tcgtcgccgc gaccatcggc
      781 ctggcccaga acgacatcaa gcgcgtcctc gcctactcga cggtcagcca gctcggctac
      841 atgttcctcg cctgcggcgt agcctcgttc acggccggca tcttccacct gatgacccat
      901 gccttcttca aagccctgct cttcctcggc gccggttcgg tcatccatgc gatgagcgac
      961 aaacaggaca tgcgcgagat gggcggactg cggaaacatc tgccggtgac ctacgccacc
     1021 atgctcgtcg ccaccctggc gatcgccggc atccccggct tctcaggatt cttcagcaag
     1081 gacgagatcc tctggaaatc gttctcgtcg gcccacggcc acccgctgtt ctgggtgatc
     1141 ggcttcgcca ctgccggtct caccgccttt tacatgttcc gcctggtctt cctgaccttc
     1201 ttcggaaaag agcggatgga ccagcacacg aaggaccacc tccacgagtt cccccggtcg
     1261 atgacggttc cgctggtgat ccttgcggtg ctgtcggtgg tcggcggata tgtcggcatc
     1321 ccgcacgtct tcggcgcgac caactggttc gagcgctggc tggaaccggt gatggccggc
     1381 ggcggggggg aagcggcgcc ggagacggtg cacgcctcgg ccggcggcta cgggaccggg
     1441 atggagcttg ccctcatggg cgcctccgtg gccctcgtgc tcctggccat ctatggcgcg
     1501 cacctgttct accgcaagcg gacggagctg gccacccgct ggcgggagcg cctctccggt
     1561 ctgcaccgcc tcctggtcaa caagtactac gtcgatgaga tctatttggc cgtgatcgtc
     1621 cggcccctgg tcgggttatc gatgttcctg tggaagttcg tggatgtgat cgtgatcgac
     1681 ggactcgcca acggcagcgc tgcggcctac cagggcctgt ccgagggtgt tcgccgcctc
     1741 cagaccggcc gcgtccggag ctacgccacg ctcttcgtgc tcggcgtcgt cctcctggtc
     1801 gcctactttg caatggattg gggatggaaa atcaaattct gacctacgtc acattcttcc
     1861 cgctcgtcgg gattgtcgct ctcctgctgg cgccgaagga acggcacgac acgatcaagg
     1921 cggtcgcgct catcgtggcc gtcatcacca tgctcctgtc gttcttcctc tggtcaatgt
     1981 tcgacccgat ggcgctgggg atgcagttcg aagtcgacat cccgtggatc accgggctgg
     2041 gcatccacta ccacctcggg atcgacggca tctcgctgct gctgatcgcg ctgacgaccg
     2101 tcctgaccgt tctggccatc ctcgcctcgt ggaactcgat caccaagggg gtgaaggggt
     2161 actatctctc gatgctcctg ctgcaggtgg ggatgatcgg cgtgttcgtg tcactggatc
     2221 tcttcctgtt ctacgtgttc tgggagatca tgctggtccc gatgtatttc atcatcggtc
     2281 tgtggggcgg cccccggcgg gtgtacgccg cgatcaagtt cgtcctcttc accatgttcg
     2341 gctcgctcct catgctcgtc ggcatcctct ggctgctctt cgcctaccac ggctactccg
     2401 gggagtattc gtttgacatg ctcaagctcg tggagatgcc gatcggctac cgggcgcagc
     2461 tcttgttgtt cggggccttc gcgctggcct tcgcaatcaa ggtcccgctc tttcccttcc
     2521 acacctggct gcccgacgcc cacgtggagg cgccgacggc cggctcggtc atcctcgcgg
     2581 gcgtgctgct gaagatgggg acgtacggtt tcgtgcgcat ctgcctgccg atgtttccgg
     2641 acgccaccgt ggcctacctc ccctacatcg ccgtgctgtc ggtcatcgcg atcatctacg
     2701 gcgcgctcgt ggcgatggtg cagcgggacg tgaaatcgct cgtggcgttc tcctcggtct
     2761 cccacatggg cttcatcatg ctggggatct tcgccctcaa cctgcaggga ctccagggcg
     2821 cgatcatgca gatggtcaat cacggcgttt ccacgggcgc cctgttcctc ctggtgggga
     2881 tgatctacga ccgccgccac acgcgcctca ttgaggaatt cggcgggctg gccaaagtca
     2941 tgccggccta ctcggccatc ttcatgatcg tcgccctctc ctcgatcggc ctgccgttca
     3001 ccaacgggtt cgtcggcgag ttcctcatac tcctcggcgc gttcgaccgc agctatgtct
     3061 acggcaccct ggcggcctcg ggcgtgatct tcgccgccgc ctatatgctc tgg
//
LOCUS       DAOOKS010000171         3112 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_38041_length_3112_cov_2.694145, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000171 DAOOKS010000000
VERSION     DAOOKS010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38041_length_3112_cov_2.694145"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="PK112_03625"
     CDS             <1..382
                     /locus_tag="PK112_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018047040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPC11173.1"
                     /translation="FGFKGAAQQKMPSELSGGERNRCLLAKVLKSGGNVLLLDEPTND
                     LDVNTLRMLEEAILDFSGCVLVISHDRFFLDRIGTHLLVFEGEGKVRWFEGTFSEYEE
                     WRIREMGDRLFENRRNRYRTIVRA"
     gene            473..1282
                     /locus_tag="PK112_03630"
     CDS             473..1282
                     /locus_tag="PK112_03630"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HPC11174.1"
                     /translation="MPQIRATRLEKGEGRAETWLSRYLPYGTRRRKIALWAGLPLAIL
                     LVLYFIMDNIVMMQVTRHGEEFTLPNFEGQTMIEASVTLDNLGLSGEVASYEFAPGTP
                     KGVIINQFPRAATKVKDGRRIKFVVSAGQKMITIPSLAGESVRQAMLDLETAGLKLGE
                     IAWAFSDTLPERVVVFSYPAAGTEIPLGAPVNLMVNRGRASSFTYVPHVVGLTLAEAQ
                     KRIEDKNLRVGVVTYKKDDAYLPETVLEQSDPPGAELDIHTEIDLVVSKTD"
     gene            1341..2201
                     /locus_tag="PK112_03635"
     CDS             1341..2201
                     /locus_tag="PK112_03635"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HPC11175.1"
                     /translation="MPDRLLISSAPVSLTRPMQAMVAKLYSPALLPAVALERITYLSD
                     SLKVSGYLARPAAEGRYPVVIWNRGGYREHGALDDFRAMLILGSTAQWGYVVLATQYR
                     GNAGSEGTESWGDKDVDDALNLIPLAETLPFCDPRRIAIEGASRGGMTTYRALARESR
                     FRCAIVHAGLADLFALAELRGQMRSWLDELFGDLSPAEKERELGRRSAVYFADRFPDH
                     VPLLMLHGSADTRVPLSQSEALAAELARFGKPHRLVVIPGGGHVALKDGSYREVDRHR
                     KAWLAQHLVG"
     gene            2519..>3112
                     /locus_tag="PK112_03640"
     CDS             2519..>3112
                     /locus_tag="PK112_03640"
                     /inference="COORDINATES: protein motif:HMM:NF012662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HPC11176.1"
                     /translation="MFSALSDKLEDVFKKLRGAGRLSEKNIRDAMREVRQALLEADVN
                     YKVAKRFVAAVEAKAVGEEVLRSIEPGQQVIKIVHDELVLLLGGKAEPLAPLDRAPTI
                     FMVCGLQGSGKTTLCGKIALWAKRKNKKPLVVAADIYRPAAVKQLRVLAESIQVPHFS
                     LDGAAPPEICREALRHAERSFIDLVILDTAGRLHVDEE"
BASE COUNT          529 a          965 c         1072 g          546 t
ORIGIN      
        1 gttcggattc aagggcgccg cccagcagaa gatgccgagc gagctgtccg gcggcgagcg
       61 caaccgttgc ctcctggcga aagtgctcaa atccggcggc aacgtcctgc tgttggacga
      121 gccgaccaac gatctggacg tcaacaccct tcgcatgctc gaggaggcga tcctcgattt
      181 ctccggctgc gtgctcgtca tcagccacga ccggttcttc ctcgaccgca tcggcaccca
      241 cctcctcgtg ttcgagggcg aagggaaggt gcgctggttc gaggggactt tctccgagta
      301 cgaggagtgg cgaatccggg agatgggcga ccgcctgttc gagaaccggc ggaaccggta
      361 ccggacaatc gtgcgggcct gagtgcgggc cgcggtgcgg gcggcgtttt aaccttgcgc
      421 ccggcatgag aattccccac cctgccggag tataaggaat cggaaaaaga gtatgccgca
      481 gattcgcgcc acccgactgg agaagggaga aggccgggcc gagacctggc tgagccggta
      541 tctcccgtac ggcacccgcc ggcggaagat cgctctctgg gcgggtctcc cgctggccat
      601 cctcctggtg ctctatttca tcatggacaa tatcgtgatg atgcaggtga cccgccacgg
      661 cgaggaattc acgctgccca atttcgaggg gcagaccatg atcgaggcct cggtcacgct
      721 ggacaacctc ggtctcagcg gcgaggtggc ctcctacgag tttgcgccgg ggacccccaa
      781 gggggtgatc atcaaccagt tcccgcgggc cgcgaccaag gtcaaggacg gccggcggat
      841 caagtttgtc gtctcggccg gccagaagat gatcaccatc ccgtcgctcg cgggcgagtc
      901 ggtgcggcag gcgatgctgg atctggagac ggcggggctg aagctgggcg aaatcgcctg
      961 ggccttctcc gacaccctgc cggagcgcgt cgtcgtgttt tcgtacccgg cggccgggac
     1021 cgaaatcccg ctcggcgcgc ccgtgaacct gatggtcaac cgggggcggg cctccagttt
     1081 cacctatgtg ccgcacgtgg tcgggctgac gctcgccgag gcacagaagc ggatcgagga
     1141 caagaacctc cgggtcgggg tggtcacgta caagaaggac gacgcctatc ttccggagac
     1201 cgtgctggag caatcggacc cgccgggggc ggaactggac attcacaccg agatcgattt
     1261 ggtggtgagc aagacggact gaggggcggg gacccgccgc catctcgacc gcagatctca
     1321 cgattctcac ggcacccgcg atgcccgata ggcttctcat ctcctccgcc ccggtgtcgc
     1381 tgactcgccc gatgcaggcg atggtcgcga aactgtattc ccccgcgttg ctccccgcgg
     1441 tcgcgctcga acgaatcacc tatctctccg acagtctgaa agtcagcggc tacctcgcgc
     1501 ggccggcggc ggaggggcgg tacccggtcg tgatctggaa ccgcggcggc taccgggagc
     1561 acggggcgct cgatgatttt cgcgccatgc tcatcctcgg ctcgaccgcc cagtgggggt
     1621 atgtcgtgct ggcgacgcag taccggggga acgccggttc ggaggggacc gagagctggg
     1681 gggataagga tgtcgacgac gcgctcaatc tgattccgct ggccgagacg ctccccttct
     1741 gcgacccgcg ccggatcgct atcgaaggtg cgagccgcgg cgggatgacg acctatcggg
     1801 cgctcgcgcg cgaatcccgc tttcgctgcg cgatcgtgca cgccggactg gcggatctct
     1861 ttgcgctggc cgaactccgg gggcagatgc gctcgtggct ggacgagctg ttcggtgacc
     1921 tctccccggc cgagaaagag cgcgaactgg ggcggcggtc ggcggtgtat tttgcggacc
     1981 ggtttcccga ccacgtgccg ctgctcatgc ttcacggcag cgcggacacg cgcgtgccgc
     2041 tgtcgcagtc ggaggcgctg gccgccgaac tggcgcggtt cggcaagccg caccggctgg
     2101 tggtgatccc cggcggaggg catgtggcgc tcaaggacgg ctcctatcga gaggtggatc
     2161 gccatcggaa agcgtggttg gcgcagcacc tggtcgggta gggatcgggc gacggggccc
     2221 cggcccgccg ccgtaaactt acggaaccta aatcctcttg gtgcagcgat agttacgggt
     2281 gcagcaggag agcctccccg aagccgccat tcgtataaac ttcttgcggt acaaacggtt
     2341 ttggttatat tgttgagctt tgcgccgacc ggcggttgaa tgtggaccag tcggtcggac
     2401 cgaagtggtg tgtctttttc cgcccggccg cggcgttgtt gtcgacggtt ggaggaagac
     2461 ggcatggaga ctctggtgca gctcgcgtgc ggagcgggcg ttttgatgtt tcggtagtat
     2521 gtttagcgcg ttaagcgaca agctcgaaga cgtcttcaag aaacttcgcg gcgccggccg
     2581 gttgtcggag aagaacattc gcgacgccat gcgcgaggtg cgccaggcgc tgctcgaggc
     2641 ggacgtcaac tacaaggtcg ccaagcgctt tgtcgccgcc gtcgaggcca aggcggtcgg
     2701 cgaggaagtt ctccggtcga tcgaaccggg ccagcaggtg atcaagatcg tccacgacga
     2761 gctggtcctc ctgctcggcg gcaaggcgga accgctcgcg ccgctcgacc gcgcgcccac
     2821 gatcttcatg gtgtgcgggc tccagggctc gggaaagacg acgctctgcg ggaagatcgc
     2881 tctctgggcg aagcggaaga acaagaaacc gctcgtggtg gcggccgaca tctaccgccc
     2941 ggccgcggtc aagcagctgc gcgtgctggc cgagtcgatt caggtgccgc acttttcgct
     3001 cgatggcgcg gcgccgccgg aaatctgccg cgaggcgctc cgccatgccg agcgcagttt
     3061 tatcgacctc gtcatcctcg acaccgccgg ccgtctgcac gtcgacgagg ag
//
LOCUS       DAOOKS010000172         3088 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_38427_length_3088_cov_2.985493, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000172 DAOOKS010000000
VERSION     DAOOKS010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38427_length_3088_cov_2.985493"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="PK112_03645"
     CDS             complement(<1..440)
                     /locus_tag="PK112_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11177.1"
                     /translation="MKRTNRPSQLIDDTGPIHLGCQPPVAFDKDKARDRSHEPTYPHL
                     TANSRARYRSRATAEHVGDEESDIRNSQLSIRRHIRLGKAVRCRPAAKDMVDEERDIH
                     DRDLVLFIEINIPSDTVAALGPLSDLGGDQAACDGEAAACDQGAV"
     gene            683..1621
                     /locus_tag="PK112_03650"
     CDS             683..1621
                     /locus_tag="PK112_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007480930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease NucS"
                     /protein_id="HPC11178.1"
                     /translation="MKSYFRILLGSKNCFAEEAYRSNCIAAGFITDRDLTHHLPDNWR
                     DFNREFIPVWMKQNPGKPKVSAGLACGMLWTVAKGVQVGDVVLCPDGKGNYFVGEVTG
                     GYEYKKGEELPHRRVVRWFDRPLTRDEMSEPLRKSLSSGPPVINVSRHAAEIERLIAG
                     GQPLTIVSTDATVEDPSAFALEKHLEDFLVQNWHHTELGKHYDVFEEDGELAGQQYQT
                     DTGPIDILAVSKDGKEIVVIELKKGRASDVVVGQVQRYMGYVKEELAESNQNVRGVII
                     AFEDDIKLRRALAVAPNIDFYTYKVHFKLERKNQRR"
     gene            complement(2119..>3088)
                     /locus_tag="PK112_03655"
     CDS             complement(2119..>3088)
                     /locus_tag="PK112_03655"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HPC11179.1"
                     /translation="GDLITIRADIFFNSEFDTTIHNVLIRFYDGDPNVLVQQIGPDQI
                     LASIDSGQTVVLEVNWTVPDLLPHDIYIVVDPLGTIPEWDESNNRAYRTWVPGQCEED
                     RLVVIPWVQYEFYARHIGPLTLDSLCLWADDSCTWLQTPITVNPLQSYAWIGNFADPY
                     GPDDVDTATLMINDAVPLHGFAADLVGSPESFPFLDQIFPGQLVLRMRYSIPALVQYY
                     IDSLAALPEPELMWDEAVYPLAVKGQYLDGTAFNLCTDLIIIGHRSGDVNGDDSRTVT
                     DITFLIGYLFRGGPAPDPVYLGDVNHDGQTTVADITRLVGIIFRGE"
BASE COUNT          642 a          859 c         1010 g          577 t
ORIGIN      
        1 accgcaccct gatcgcaggc ggccgcttca ccgtcgcagg cagcctggtc tccgcctaaa
       61 tcgctgagtg gaccaagtgc tgcaacggta tcagagggaa tgttgatttc gatgaagagg
      121 acaaggtcac ggtcgtggat atcacgttcc tcatcaacca tgtctttcgc ggcgggcctg
      181 caccggactg ccttgccgag gcggatgtga cggcggatgg aaagttggct gttgcggata
      241 tcactttcct catcgcctac gtgttccgcg gtggcccggc tccggtagcg tgcccgtgag
      301 ttggcggtga gatgcggata tgtaggctcg tggcttcggt cacgggcctt gtctttgtca
      361 aacgcgacgg gcggctggca gccgagatgg atgggacccg tgtcgtcgat cagctgcgaa
      421 gggcggttcg tgcgtttcat tctacgcgag agcataagga atccgtagtg tgcgactacc
      481 acccacgttt ctcctccccg tggcggggcc gaatcgagct aaggagcggc cagcgtcaga
      541 gcgcggagtc tgagaaagcg gttgcctcat tgtacagtgc tcgttaattt gtggcgtggt
      601 cataatcgag cggtagcaca acagcgagag ttctgggtat cgcgcaagag cacataaatg
      661 gaatgaggct caaatccgca tcatgaaaag ctatttccgc attctgcttg gcagtaagaa
      721 ctgttttgct gaagaggcgt acaggagcaa ctgcatcgcg gccggattca tcaccgaccg
      781 agacctgaca catcatttgc ccgacaactg gcgggatttc aacagagagt tcatccctgt
      841 ctggatgaag caaaaccctg gcaagcccaa ggtatcggcc ggcctggctt gcggcatgct
      901 gtggaccgtg gccaagggcg ttcaggtagg ggatgtcgta ctctgccccg acggcaaggg
      961 gaattacttc gtcggtgagg tgacgggcgg atatgagtac aagaagggag aagaactgcc
     1021 gcaccgccgc gtggtgcgct ggttcgaccg gcccttgacc cgtgacgaga tgagtgagcc
     1081 gctccgaaaa tcgctttcgt cgggccctcc ggtcatcaac gtctcccggc atgccgccga
     1141 gatcgagcgg ctcatcgccg ggggccaacc actgaccatt gtcagtactg acgcgaccgt
     1201 tgaagatccg agcgctttcg cgttggagaa acacctggaa gacttccttg tacagaactg
     1261 gcatcatacg gagctcggta agcactacga cgtattcgag gaggacgggg agttggcagg
     1321 gcagcagtac cagaccgaca ccggaccgat tgacattctg gcggtcagca aggatggaaa
     1381 ggaaattgtc gtcattgaac tcaagaaggg cagggcgagc gacgtggtgg tcgggcaggt
     1441 gcagcggtac atgggatacg tgaaggagga gctggcggaa tcaaaccaga acgtacgcgg
     1501 ggttatcatc gccttcgaag acgacataaa gctccgtcgg gcactggccg tcgctccgaa
     1561 cattgacttc tacacgtaca aggttcattt caagctcgaa aggaagaacc agcggcgatg
     1621 aatgcggtgg gacacgatgc gtgcgcgatg tcggcatcct tagaccgtcg gccgggccgc
     1681 gcgactgagg tgcttgtgtc cattttgtgc ccacgtgagg gtcaaaacct ccgcgtccga
     1741 cagaactcga tacaaaagca taaaaacgcc taaacccatc ccggataatg gcttacggcg
     1801 caacccgctt aacggcatag cattacagaa acgctgcctg tagagtcccc ccttcggcat
     1861 gatcttctcc tcccctctct cctctcccct acggatctcg tggtttgcgc ggcggcgtgc
     1921 ggcgtgcgga agcggggcat ggtcccggcc cataagagag gcgatcggcg ggaggatcag
     1981 cggggggatc ggcgggataa tcggcggagc cctcggccgt cgcctgcggc cacaaacgaa
     2041 accggccgga gccgaagctc cagccggtta gtgtgagccg attggcgcgc ggccgtgccg
     2101 cgcccgcggt cgggacggct actcgccccg gaagatgatc ccgacaaggc gtgtgatatc
     2161 agccacagtg gtctgaccgt cgtggttgac gtcgccgaga tacaccggat ccggcgccgg
     2221 gccgccccgg aacaggtagc cgatcaggaa cgtgatatcg gtgaccgtcc ggctgtcatc
     2281 gccgttgacg tcgcccgagc ggtggccgat gatgatcagg tcggtgcaca ggttgaacgc
     2341 ggtgccgtcg aggtactgcc ccttcacagc cagcggatag accgcttcgt cccacatgag
     2401 ttccggttca gggagagcgg ccagcgagtc gatgtagtac tgcacgaggg cgggaatact
     2461 gtagcgcatg cgcagcacca actggccggg gaaaatctgg tcaaggaacg ggaacgactc
     2521 gggggagccg accaggtcgg cggcgaaccc gtggagcggg accgcatcgt tgatcatgag
     2581 cgtcgccgtg tcgacatcgt ccggcccgta agggtctgcg aaattgccga tccaggcata
     2641 agactggagc ggattaaccg tgatcggcgt ctggagccag gtgcaggaat cgtccgccca
     2701 caggcacagg gaatcgagcg tcagcggccc gatgtggcgg gcgtagaact cgtactgcac
     2761 ccaggggatg accaccaggc ggtcctcctc gcactggccc gggacccagg tccggtacgc
     2821 ccggttgttg ctctcgtccc attccgggat ggtgcccaac gggtcgacga cgatgtagat
     2881 gtcgtggggg agcaggtcgg gaaccgtcca gttgacctcg agcacgacgg tctgaccgga
     2941 atcgatgctc gccagaattt gatcggggcc gatctgctgg acgagaacgt tcgggtcccc
     3001 atcgtagaaa cggatcagca cattatggat ggtcgtgtcg aactcggagt tgaagaagat
     3061 gtccgctcgg atggtgatga gatccccc
//
LOCUS       DAOOKS010000173         3088 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_38428_length_3088_cov_2.868777, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000173 DAOOKS010000000
VERSION     DAOOKS010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38428_length_3088_cov_2.868777"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="PK112_03660"
     CDS             complement(<1..354)
                     /locus_tag="PK112_03660"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPC11180.1"
                     /translation="MTERQRPAPDRLDENEVLRLVRRGAATDTFRRLPAEKKAFIYRT
                     ALRLFGEFGYDGLAVDRFCREAGISKGSFFQYFPSKSHLLEFCVLLFDRSLGEWLADI
                     RRTDGGGLARQRLLHL"
     gene            524..1747
                     /locus_tag="PK112_03665"
     CDS             524..1747
                     /locus_tag="PK112_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HPC11181.1"
                     /translation="MGKIRVAVIGVGNCASSLIQGVEFYKKAREDEKVPGLMHVNLGG
                     YHISDIEFVAAIDIDKNKVGKDLAEAIYTWPNNTYQFCKVPKTGIIVQRGMTHDGLGF
                     YLSQIIEKAPGDTVDIVKLLKDTKTDVVVNYLPVGSEEATKWYVEQILLAGCGFINCI
                     PVFIAKEQYWQKRFKEKGLPVIGDDIKSQVGATIIHRIMTRLFMDRGVKLERTSQLNV
                     GGNTDFLNMLERSRLQSKKISKTSAVTSMLDYDIGAKNVYIGPSDYVEWLDDRKWAHI
                     RLEGTTFGNVPLNVEMKLEVWDSPNSAGVVIDAIRCCKLGLDNGLSGALIAPSAYFKK
                     SPPVQYPDDVARQMVEQFIAEYGWKQKTTAVRPGVRAAAKDRKTARPATGARPAGGGK
                     KPAAAAPKRIIRKKK"
     gene            1830..2477
                     /gene="tmk"
                     /locus_tag="PK112_03670"
     CDS             1830..2477
                     /gene="tmk"
                     /locus_tag="PK112_03670"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019239214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HPC11182.1"
                     /translation="MSKNRRGLFISFEGIDGCGKTTQVMLVYKYLIAQGRQVKLVREP
                     GSTEAAERIRALLLDRRLAIDERTELLLYEAARADLAAREIAPALARGLVVLCDRFYD
                     STTAYQGFGRRLDVAMVRALHRVAVDDLEPDLTLVFDCDIRTAMGRRAGDPDRLEAES
                     RAFFERVRKGFLEIARKERRRVKVIDAAQPVETVFADVKKHLVRKLRNHDAERRP"
     gene            2455..>3088
                     /locus_tag="PK112_03675"
     CDS             2455..>3088
                     /locus_tag="PK112_03675"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HPC11183.1"
                     /translation="MMPSAAPDRVDLLISMVVIVLLVALAGGMGVYVVSDPGLRAAAP
                     LVRAAEVIRSRWVEPVDGARLTEAGRRAMFDLLDRYSGYVDRTDFDRMREELSGAYGG
                     IGVTIVPHDGGLLIMSVREDGPAGRVGLLTGDILVGTDSVPFARLTPAEASALLRGRP
                     GTDVRVRVYRPVAGDTITVALTRQRIPLVHVPFAGLTDRNIIYIRVLDFDA"
BASE COUNT          554 a         1055 c          988 g          491 t
ORIGIN      
        1 gaggtgcagc agccgctgcc gggccagccc gcccccgtcg gttcgccgga tgtccgcgag
       61 ccactccccc agcgaacggt cgaagagcag cacgcagaac tccaggaggt ggctcttgga
      121 ggggaagtac tggaagaacg agcctttgct gattcccgcc tcgcggcaga accggtccac
      181 cgcaagaccg tcgtaaccga actccccgaa cagccggagc gcggtccggt aaatgaacgc
      241 cttcttctcg gccggcaggc ggcggaaagt atcggtggcg gcgccgcgcc gcaccagccg
      301 gaggacttca ttttcgtcga gccggtcggg cgccggccgc tgtcgctcgg tcatacggcc
      361 ctccacagga tatgactgtt tggtcgcctg tgatatacta ttgttctata atgacggcaa
      421 gcggtttctt tcaatttgac aggggcggcg gccgatctct atattcgcgg cgaaaaaggc
      481 aaacgggccg caccggcccg ccagagcagg aaggaagaat ggtatgggaa aaatcagagt
      541 tgcggtcatc ggcgtcggca actgcgcctc atcgctcatc cagggcgttg agttctacaa
      601 gaaggcccgc gaggacgaga aggtcccggg gctcatgcac gtcaacctcg gcggctacca
      661 catcagcgat atcgagttcg tggccgccat cgacatcgac aagaacaagg tcggcaagga
      721 tctcgccgag gcgatctata cctggccgaa caacacctac cagttttgca aggtgccgaa
      781 aacgggcatc atcgtccagc gcggcatgac gcacgacgga ctggggttct acctgtcgca
      841 gatcatcgag aaggcccccg gcgacaccgt cgacattgtc aaactcctga aagacaccaa
      901 gaccgacgtc gtcgtgaact acctcccggt cggctcggag gaggccacca agtggtacgt
      961 cgagcagatc ctcctggccg gctgcggctt catcaactgc atcccggtct tcatcgccaa
     1021 ggagcagtac tggcagaagc ggttcaagga gaaggggctg ccggtgatcg gcgacgacat
     1081 caagtcgcag gtcggcgcca ccatcatcca ccgcatcatg acccggctgt tcatggaccg
     1141 cggcgtcaag ctcgagcgca ccagccagct caacgtcggc ggcaacaccg acttcctcaa
     1201 catgctcgag cggtcgcgcc tgcagtccaa gaagatctcc aagaccagcg ccgtcaccag
     1261 catgctcgac tacgacatcg gcgcgaaaaa cgtctacatc ggcccctccg actacgtgga
     1321 gtggctcgac gaccggaagt gggcgcacat ccggctggag ggcacgacgt tcggcaatgt
     1381 cccgctcaac gtcgaaatga aactcgaggt ctgggacagc ccgaactcgg cgggggtcgt
     1441 gatcgatgcg atccgctgct gtaagctcgg cctggacaac ggcctctcgg gcgccctcat
     1501 cgccccctcg gcctacttca agaaatcccc gccggtgcag taccccgacg acgtggcccg
     1561 gcagatggtc gagcagttca tcgccgagta cggctggaag cagaagacga cggcggtccg
     1621 gccgggcgtc cgcgccgccg ccaaggatcg caagacggcc cgccccgcga ccggcgcccg
     1681 cccggctggg ggcggaaaga aaccggcggc ggccgcgccc aagcgcatca tccgcaagaa
     1741 aaagtagttt cccgccctct ttgcgcaggc tactttaccg gcggtgggtt ccaccgccgg
     1801 tatgtttttg tggccgtcag ggtagccgtg tgagcaagaa ccgacgcgga ttgttcatct
     1861 ctttcgaggg catcgacggg tgcggcaaga ccacccaggt gatgctcgtc tacaaatacc
     1921 tgatcgcgca ggggcgccag gtcaagctgg tgcgcgagcc cgggtcgacc gaggcggccg
     1981 agcggatccg cgccctcctc cttgaccgcc gcctggccat cgacgagcgc accgagctgc
     2041 tgctctacga ggcggcgcgg gccgatctgg cagcccggga gatcgccccg gcgctcgcgc
     2101 gcggcctggt ggtcttgtgc gaccgcttct acgacagcac caccgcctac cagggtttcg
     2161 gccggcggct ggatgtcgcc atggtgcggg cccttcaccg ggtggcggtg gacgatctcg
     2221 aacccgacct gacgctggtg ttcgactgcg acattcgcac cgccatgggc cgccgggcgg
     2281 gcgaccccga ccggctcgag gccgaatcgc gcgccttctt cgagcgggtt cgcaaggggt
     2341 tcctcgaaat cgcgcgcaag gagcgccgcc gcgtcaaagt gatcgacgcc gcccagccgg
     2401 tggagaccgt gttcgccgac gtcaagaaac acctcgtgcg caaactgaga aatcatgatg
     2461 ccgagcgccg cccctgaccg ggtcgacctg ttgatctcga tggtcgtcat cgtgctgctg
     2521 gtcgcgctcg ccggcggcat gggcgtgtac gtcgtgtccg acccgggcct gcgcgccgcc
     2581 gcgcccctgg tgcgggccgc cgaggtgatc cgctcacgct gggtggagcc ggtcgacggc
     2641 gcgcgcctga ccgaggccgg ccgccgcgcc atgttcgacc tgctcgaccg ctactcgggc
     2701 tacgtcgacc gcaccgactt cgaccgcatg cgcgaggaac tctccggtgc ctacggcggc
     2761 atcggcgtga ccatcgtccc ccacgacggc ggactgctga tcatgtcggt gcgcgaggac
     2821 ggaccggcgg gccgcgtcgg gctgctcacc ggcgacatcc tggtcggcac cgattccgtg
     2881 cccttcgccc ggctcacccc ggccgaggcc agcgccctgc tccggggtcg gcccggcacc
     2941 gacgtgcgcg tccgggtcta ccggccggtg gccggcgaca cgatcacggt cgctctcacc
     3001 cgccaacgta tcccgctcgt ccacgtgccc ttcgccggcc tcaccgaccg caacataatc
     3061 tacatccgcg tgctcgattt cgatgccg
//
LOCUS       DAOOKS010000174         3078 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_38600_length_3078_cov_3.691697, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000174 DAOOKS010000000
VERSION     DAOOKS010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38600_length_3078_cov_3.691697"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1271
                     /gene="dnaA"
                     /locus_tag="PK112_03680"
     CDS             <1..1271
                     /gene="dnaA"
                     /locus_tag="PK112_03680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HPC11184.1"
                     /translation="NGDGQFYLAVPNQFVADWIKGHFGEVIDEAFREVLGSSYQLEFV
                     IAQIPEVPEQNALNLELPLPERPEPLPVPQTQPLGRRHDHNLNRKYTFDSLVVGDFNQ
                     FAFAAAQAVAEAPGLNKYNPLFIYGGTGLGKTHIVQAIGNAILEAFPNKRIFYATSEK
                     FTSDFIASISDRTIADFTKVYRDVDVLIIDDIQFFSGKESTQEQFFHTFNSLHHLGKM
                     IVLTADRPPREIKGLEERLLSRFQWGLVADLQAPDLENRTAILYKKLETEGITLPDNI
                     VRYIADQVQTNIRELEGALIRLLAYASLRQEEVDLKLAEKVLGGGNSVKCKVIEIEAI
                     QRKAAEYFKVEPHMMQAKKKTADVVLARQVAMYLCRSLTSNSLKVIGAAFGGRDHSTV
                     IHACDLISRRMAREPDLRHKIDQISASLLY"
     gene            1558..2670
                     /gene="dnaN"
                     /locus_tag="PK112_03685"
     CDS             1558..2670
                     /gene="dnaN"
                     /locus_tag="PK112_03685"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HPC11185.1"
                     /translation="MKFTLPKSRLGQYLQHILQVVPAKSTLPILSNILLEALENKLKV
                     SATDLDISITASLDATVTKKGAAVVPARILFEIIKELPESEITVETVGSRVELKVPNG
                     SYKIGSVTPEDFPKLPAVNTKKEIRIVAGSLVNMIRKTTFACSDDETRPALNGVLWQT
                     KGEKMQMVATDGHRLAKMATENRQLKGMYEDVIIPPKVLNLIPKFIAGDDQEIGIIFG
                     ENNIIFNLSDIILTSRLLEGPYPNFEQVIPQDNDKRLTIHKDDLAGAVRRVAILSNAL
                     THQVKFSLKDNTLVLSTTNADVSGEGKETLECDYKGDAIELGYNANYITEILSKIDGD
                     EVIFELATSVSAGIVYSPDVAKEDFLCLVMPLRLAD"
     gene            2701..>3078
                     /locus_tag="PK112_03690"
     CDS             2701..>3078
                     /locus_tag="PK112_03690"
                     /inference="COORDINATES: protein motif:HMM:NF024904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPC11186.1"
                     /translation="MAASAIFHFGMILRSLHLQGFRNFAPLAIEFGERVNILYGDNGS
                     GKTNLIEAIFVLCLGRSHRGVPDSVMLQQGTETFRLEGRLSRNGETEAVAVAYQTGGR
                     KKITVEGVPIRPAELYDRFCAVTA"
BASE COUNT          698 a          877 c          917 g          586 t
ORIGIN      
        1 tcaacggcga cggccagttc tacctggcgg tgccgaacca gttcgtggcc gactggatca
       61 agggccactt cggggaggtt atcgatgaag cgttccggga ggtgctgggg tcgagttatc
      121 agttggagtt tgtgatcgcg cagataccgg aggtgccgga gcagaacgcg ctcaacctgg
      181 aactgcccct gcccgagcgg ccggagccgc tgccggttcc gcaaacgcag ccccttgggc
      241 ggcggcacga tcacaatctg aaccggaagt acacgtttga ctcgctcgtg gtcggtgatt
      301 tcaaccagtt cgcgttcgca gccgcccagg cggtcgccga ggcgcccggt ctgaacaagt
      361 acaacccgct gttcatttac ggcggcaccg gcttggggaa aacccacatt gtccaggcca
      421 tcgggaatgc gatcctcgaa gctttcccca acaagcggat cttctacgcc accagcgaga
      481 aattcacttc cgatttcatc gcctccatct ccgaccgcac gatcgccgat ttcactaagg
      541 tctaccgcga cgtggacgtc ctcatcatcg acgacatcca gttcttctcc ggcaaagagt
      601 ccacgcagga gcagttcttc cacactttca acagcctcca ccacctgggg aagatgattg
      661 tgctcactgc cgaccgtccg ccgcgcgaga tcaagggtct ggaggagcgc ctgctgtcgc
      721 gcttccagtg gggcctggtg gccgacctcc aggcgcccga cctggaaaac cgcaccgcca
      781 tcctctacaa gaagctggag acggaaggga tcacgctccc cgacaacatc gtgcgctata
      841 tcgccgacca ggtgcagacc aacatccgcg agctcgaggg ggcgctcatc cgcctgctcg
      901 cgtatgcttc cctgcggcag gaggaggtcg acctgaaact ggcggagaaa gtcctcggtg
      961 gcggcaactc ggtgaaatgc aaagtaatcg agatcgaggc gatccagcga aaagccgccg
     1021 agtacttcaa ggtggagccg cacatgatgc aggccaagaa gaaaaccgcc gatgtagtcc
     1081 tcgcccgcca ggtggccatg tacctctgcc gctcgctcac gagcaactcg ctcaaagtga
     1141 tcggggcggc cttcggcggc cgggaccact ccaccgtcat ccacgcctgc gacctgatct
     1201 cgcgccgcat ggcccgggag ccggatctcc ggcacaaaat cgaccaaatc tcggcctcgt
     1261 tgctgtacta acaagatgtg gagtgaagtt gtggaagatg tgcgcacagg tcggcttgga
     1321 cgcgaactgt ggaagaagtc ggaaagggag aggttgttgt ggggataacg ctgggataaa
     1381 aaaaggatgg ggatttgttt taaattgttg ccatgcaaag tgtacgggtg acatattagt
     1441 ggaaggcgag gggttgtaca caaccccaca gcccttatta atgttactcg tgagataagt
     1501 agaaggatat tgtaagagac aacgtgggga taggctacgt tgcaggagag cacggagatg
     1561 aaattcacct taccgaagtc acgactgggg cagtacctcc aacatatcct ccaagtagtg
     1621 cccgcaaagt cgacccttcc gattttgtcg aatattctgc tcgaagcgct ggagaacaaa
     1681 ctgaaagtct ccgccacgga tctcgatatc tcgattaccg cctccctcga tgcgaccgtc
     1741 acgaaaaaag gggcggccgt tgttcctgcg cgtattctct tcgagatcat caaggaactt
     1801 ccggagtcgg agatcacggt cgagaccgta gggtcacggg tggaactgaa agtcccgaac
     1861 ggctcgtaca agatcggttc ggtaacgccg gaggactttc ccaagctgcc ggccgtcaac
     1921 acgaaaaagg aaatacgcat cgtggcgggc agcctcgtca acatgatccg taaaaccacg
     1981 ttcgcctgtt cggatgatga gacgcggccg gccctcaacg gagtgctctg gcaaacgaag
     2041 ggggagaaaa tgcagatggt ggccaccgat ggccaccgct tggccaagat ggccacggag
     2101 aacagacagc tgaaggggat gtacgaggat gttatcattc caccgaaggt gctgaatctc
     2161 attcccaagt ttatcgcagg cgatgatcag gagatcggga tcatcttcgg ggagaacaac
     2221 atcatcttca acctcagcga cattattctc acttcccgtc tccttgaggg gccctacccg
     2281 aatttcgagc aggtaatccc gcaggacaat gacaagcggc ttacgattca caaggacgac
     2341 cttgccgggg cggtacgccg ggtggcgatt ctctccaatg ctctgacaca ccaggtaaag
     2401 ttctccctga aagacaacac gctggtgctc tcgacaacca acgccgatgt cagcggcgag
     2461 gggaaagaga cgctggagtg cgactacaag ggggatgcga tcgagctcgg ctacaacgcg
     2521 aactacatca cggagatcct ctcgaaaatt gacggtgacg aggtgatatt cgagctggcc
     2581 acctcggtct cggccggcat cgtgtacagc ccggatgtag caaaagagga tttcctgtgc
     2641 ctcgtaatgc cgctgcggct ggctgactga cgatgaaagg cggattctga ataatggcag
     2701 atggcagcgt ctgccatttt tcattttggt atgatactcc gctcccttca ccttcagggt
     2761 tttcgcaact tcgccccgct ggcgatcgaa ttcggcgagc gggtcaacat tctgtacggc
     2821 gacaacggct cggggaagac caacctcatc gaggcgatct tcgtgctctg cctgggccgt
     2881 tcccaccggg gagtgcccga cagcgtgatg ctgcagcagg ggaccgagac tttccgtctt
     2941 gaggggcggc tcagccgcaa cggggaaacg gaggcggtcg cggtggccta ccagaccggc
     3001 ggccggaaga agatcacggt cgagggagtg ccgattcggc cggccgagct gtacgaccgg
     3061 ttctgcgcgg tcacggcc
//
LOCUS       DAOOKS010000175         3064 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_38813_length_3064_cov_3.469591, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000175 DAOOKS010000000
VERSION     DAOOKS010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38813_length_3064_cov_3.469591"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            200..1126
                     /locus_tag="PK112_03695"
     CDS             200..1126
                     /locus_tag="PK112_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPC11187.1"
                     /translation="MRFLVTGGAGFIGSNLAAALAADGNQVRILDNFSSGRQENLADL
                     PASVEVVDGDIRDYWTVVQAVAGVDYVLHQAALPSVPRSVKNPLTSNEVNVGGTLNVL
                     EAAKNARVKKFVMASSSSVYGESEELPKHEGMRPSPLSPYAITKLTCEYYCKVYWELY
                     GFPTVALRYFNIFGPKQDPASQYAAVVPRFITALMRNESPTVFGDGEQSRDFTYIDNC
                     VQANILAATNDRIKGDYFNVACGGQFTLNELLDRLRRIIGTSTQAAYVDPRPGDIRHS
                     FAAIDKLGAFGYKPSVSFDEGLRRTVAFFKAK"
     gene            1312..2397
                     /locus_tag="PK112_03700"
     CDS             1312..2397
                     /locus_tag="PK112_03700"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPC11188.1"
                     /translation="MVVSGIGKRALLFCGAVLAAGVPAAAAEVAGRVYDQASGEAIPG
                     AVVRVGNSLVQLTVADTTDAEGEYLVSGLASGTDYTVMASAAGYQSAALTMAAVPLTH
                     DFALRSFADGYLVGTINQGTVHYAYAGGACYRSGGEVEAEEGFFPHIRVPSASVDSLV
                     GLIGVDPAATPTSDSLVWEACRRVWHWLAVNARAAGSHPQWTEANNFLMGYDDGWPSV
                     EAMAETYFQFGFLPWGTCMSRAHILASLLFTAGLPDRDLGIAETRWMFRYSQHMFAAV
                     RVNLRWLYLDATRMVGELPDYAALASCPVDGSEDIDYCYPYSLKVIPGTAAAGVPLLT
                     HRTSNSRHLCVAAPPDGAVTLCPAIAV"
     gene            2617..2823
                     /locus_tag="PK112_03705"
                     /pseudo
     CDS             2617..2823
                     /locus_tag="PK112_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009419634.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
BASE COUNT          510 a          932 c         1115 g          507 t
ORIGIN      
        1 gccgggaaga aggcgccgga gtcgacccgg cgcgggccgc cggagaaccg tgatttcgct
       61 ttacagcggg aaaccgatcc cgtagcttgg actgcttctc aggcaggctc tgccgactac
      121 tgcctggtgc gctaacatcg ggagccgaac agccgatagt catgggagaa ggacggcaac
      181 gtcaagaagg gacgatggta tgcgttttct agtgaccggg ggagcgggtt ttatcggatc
      241 gaatctcgcc gcggcgctgg cggcggacgg gaaccaggtc cggatcctgg acaatttctc
      301 gtccggccgg caggagaatc tcgccgatct gcccgcgagc gtggaggtgg tcgacgggga
      361 cattcgcgac tactggacgg tggtgcaggc ggtggccggg gtcgactacg tgctccacca
      421 ggcggcgctg ccgtcggtgc cccggtcggt gaagaacccg ctcacgtcca acgaggtcaa
      481 tgtcggcggc acgctgaacg tgctcgaggc ggcgaagaac gcccgggtga agaagttcgt
      541 catggcgtca tcgtcctcgg tatacggcga gtcggaggaa ctccccaagc acgaggggat
      601 gcggccgagc ccgctgtctc cctatgcgat cacgaaactg acctgcgagt actactgcaa
      661 agtctactgg gagctgtatg ggtttccgac ggtggcgctg cggtatttca acattttcgg
      721 accgaagcag gatccggcga gccagtacgc ggcggtggtt ccgcgcttca tcacggcgct
      781 gatgcggaac gagtcgccga ccgtgttcgg cgacggggag cagtcgcgcg atttcaccta
      841 tatcgacaac tgcgtgcagg ccaacatcct cgccgcgacc aacgaccgga tcaaagggga
      901 ttatttcaac gtcgcctgcg gcgggcagtt cacgctcaac gagctgctgg accgcctgcg
      961 ccgcattatc gggacgagca cccaggccgc ctacgtcgat ccccggccgg gagatatcag
     1021 acactccttc gccgcgatcg acaaactggg agcgttcggg tacaagccga gcgtgtcgtt
     1081 cgacgaaggg ttgcggcgca cggtggcgtt cttcaaagcg aaataagcgc cgccttctcc
     1141 gacttcaaat cagagagcac gagtccacgg ccccggcgcc cgccggggcc gtcttctttt
     1201 ctattggagc ctttgatcca tgaactatat tggagatagt cccggcgcgg cgggcggaag
     1261 gtccgaggac ccgggcgggg cgccggcgat tcggtcaagc ggaggtgtgc gatggttgtt
     1321 tcgggtatcg gaaagcgcgc gcttttgttt tgcggcgcgg tgctggccgc cggggtgccg
     1381 gcggccgccg ccgaggtcgc ggggcgggtg tacgaccagg cgagcggcga ggccatcccc
     1441 ggcgccgtgg tgcgggtggg caattcgctg gtccagttga cggtcgcgga caccaccgac
     1501 gcggaggggg aatacctcgt gagcgggctg gcgagcggca cggattacac ggtgatggcc
     1561 tcggcggcgg gctaccaatc ggccgcgctg actatggccg cagtgcccct cacccacgac
     1621 ttcgccctgc gatccttcgc cgacggctac ctggtcggga ccatcaatca ggggacggtg
     1681 cactacgcgt acgccggtgg ggcctgctat cggagcggcg gggaggtgga ggcggaggaa
     1741 ggattcttcc cgcacatccg ggtgccctcg gcaagcgtgg attcgctcgt ggggctgatc
     1801 ggcgtagatc cggcggcgac gccgacgagc gactcgctgg tctgggaggc ctgccgcaga
     1861 gtctggcact ggctggcggt caacgcccgc gccgccggca gccaccccca gtggaccgag
     1921 gccaacaatt tcctcatggg ttacgacgac gggtggccct cggtggaggc gatggccgag
     1981 acctatttcc agttcggatt cctgccctgg ggaacgtgca tgagccgggc gcacatactc
     2041 gcctcgctgc tcttcaccgc cggcctcccg gaccgcgacc tggggatcgc cgagacgcgc
     2101 tggatgttcc ggtacagcca gcacatgttc gcggcggtga gggtgaatct ccgctggctg
     2161 tatctcgacg cgacccgcat ggtcggagaa ctccccgact acgccgctct cgcctcctgc
     2221 ccggtggatg gatcggaaga catcgactac tgctacccct actcgctcaa ggtcattccg
     2281 gggacggcgg cggccggggt gccgctgctg acgcaccgga cgagcaactc gcgccacctg
     2341 tgtgtcgcgg cgccgcccga cggggcggtg acgctgtgcc ccgcgatcgc ggtgtgagtg
     2401 cttcagtccg atcagacagc gccgggagtg aaggtcaacg ggcagatgtg tgaggcgctc
     2461 ccggatgggc tgtactgcga gctggcggcg gcggagggag cgctgccgat cgtggccgag
     2521 ggcatggcgg ccggcgtacc ggtgagagat tccatccggg tcgagcgggc cgcctgtccg
     2581 ctcgatgccg acggcgacgg ctggtgttac gggtgcgaca actgcgccgc ggtcgccaat
     2641 ccggaccaga aggatgcgga tggcgacgga atcggggacg cctgcgacaa ctgccaggat
     2701 cacgccaacg ccgatcaggt cgatgcggac aaggatgggc ggggggatgc ctgcgacaac
     2761 tgcccgatgg cggagaatcc ggaccaggcc gatgcggacg gcgacgggat cggcgacgcc
     2821 tgctgctgta cggcccgggg gaatgtcgac atggcgggtg gcatcaccgt ggccgacgtg
     2881 actcatctgg tcggctatct gttccggggc ggaccggggg cggggtgtcc ggcccacgcc
     2941 gatgtgaacg ggaacggggg gacggaagtg gcggacctga cgcacctggt ggcgtatctt
     3001 ttccgggcgg gaccgccccc ggcggggtgc tgaagaacgc cggggcgaac tcttccgcca
     3061 tttg
//
LOCUS       DAOOKS010000176         3062 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_38846_length_3062_cov_2.981044, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000176 DAOOKS010000000
VERSION     DAOOKS010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38846_length_3062_cov_2.981044"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1613)
                     /locus_tag="PK112_03710"
     CDS             complement(<1..1613)
                     /locus_tag="PK112_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HPC11189.1"
                     /translation="MSADQSSNGTPKVGVYVCHCGINIAHTVDVKACTDFAASLPYVT
                     VAKEYKFMCSDPGQEIIQQDIREGKVNRVVVASCSPLMHENTFRKATAVGGGNAFMFQ
                     MANIREHVSWVTLDTAMATEKAKALIAGAVTRIVRHVPLQRNRVPVHPDVMIVGGGIA
                     GIHAALTMADAGKKVYLVEREPTIGGHMAKFDKTFPTLDCSACILTPKMSAVRSHPNI
                     TLWTYAEVAGVEGFVGNFKVKVRRKPRYVIEEQCVGCLDCIDACIYKTAKVPDEFNVG
                     LSKRKPVYIPFPQATPLVAVIDPETCIEFKTHRCKKSCLEVCQKNAIDFSQTEKIEEV
                     PVGAIILATGFKTFDARRIARYGYGKYPNVYSSLEIERMVNAAGPTGGEVTMPDGAKP
                     KAVGIVHCVGSRDAHTNRYCSRVCCMYSLKLAHLIKERTGAEVYNFYIDMRTPGKGYE
                     EFYDRLLQENVHFIRGRVAEISDWAMSAEEEGKLTIRVEDTLVGAVRRIPVDMVVLSV
                     GLEPQADADEVRRQFNISCSTEGWFLERHP"
     gene            complement(1603..2502)
                     /locus_tag="PK112_03715"
     CDS             complement(1603..2502)
                     /locus_tag="PK112_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HPC11190.1"
                     /translation="MKYLYYPGCSLKSTGKSYEESILAVFSALGRPLEELDDWNCCGA
                     TAYMSISEMKAFALSARNFAIAERQGDSDTHLVVPCAACYLGLNKAHRYLDEHPDIRK
                     TIQSALAAAGLSYENGIKVRHPLDVLANDVGLEAIKGAVTRPLAGLKVACYYGCQLVR
                     PYADFDDAHEPMVMDRLITALGGTPVAWPLKTRCCGGSLTGTVPEVGLRLSYVLLREA
                     QKRGCDVIATACPLCQFNLECYQPKMSRMYESRVNVPVVYFSQLMGTAFGLDRRPLGL
                     QRLFVPFAYREPAAAVEGGAHVG"
     gene            complement(2499..>3062)
                     /locus_tag="PK112_03720"
     CDS             complement(2499..>3062)
                     /locus_tag="PK112_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPC11191.1"
                     /translation="FADEIASIPGGDKLFRCIQCGTCSGMCPLSSYMDYTPRQIVAMI
                     RAGFRGEVLSSYTTWLCASCYACTVECPQEIKITEIMYAAKRLAIRGGMHPKRFPSQV
                     LATEFFRGVERKGRSNEGPLLVRLFLRTNPFKMFGNTGLGLGLFRRGRLSPKLESIKR
                     TGELHTMLAAIEQERMVRRTETTEATA"
BASE COUNT          507 a         1011 c          990 g          554 t
ORIGIN      
        1 ggatggcgct cgaggaacca cccctccgtg ctgcagctga tgttgaactg ccgccgcacc
       61 tcgtcggcgt ccgcctgagg ctccagcccg accgacagaa cgaccatgtc gaccgggatc
      121 cgccgcaccg ccccgaccag cgtgtcctcc acccggatcg tgagcttccc ctcctcctcg
      181 gcgctcatcg cccagtccga gatctcggcc acccggccgc ggatgaagtg gacgttctcc
      241 tgcaggagac ggtcgtagaa ctcctcgtac cccttgccgg gcgtccgcat gtcgatgtag
      301 aagttgtaga cctcggcgcc cgtccgctcc ttgatcaggt gggcgagctt gagcgagtac
      361 atgcagcaca cccgcgagca gtagcggttg gtgtgggcgt cgcggctacc aacgcagtgg
      421 acgatgccga ccgctttcgg tttggcgccg tccggcatgg tcacctcgcc gccggtcggc
      481 ccggccgcgt tgaccatccg ctcgatctcg aggctggagt agacgttcgg gtacttgccg
      541 tagccatagc gggcgatccg ccgggcgtcg aaggtcttga acccggtcgc gagaatgatc
      601 gccccgaccg gcacctcctc gatcttctcc gtctggctga aatcaatcgc gttcttctga
      661 cagacctcca ggcagctctt tttgcaccgg tgggtcttga actcgatgca ggtttccgga
      721 tcgatcacgg cgacaagcgg ggtggcctgg ggaaagggaa tgtacaccgg cttgcgcttg
      781 ctcagcccga cgttgaattc gtcgggcacc ttggccgtct tgtagatgca ggcgtcaatg
      841 cagtcgaggc agccgacgca ctgctcctcg atcacgtagc gcggtttgcg ccgcactttc
      901 actttgaaat tccccacgaa tccctcgacc ccggccacct ccgcgtaggt ccagagggtg
      961 atgttggggt gcgaccggac ggccgacatc ttgggggtga ggatgcaggc ggagcagtcg
     1021 agggtgggga aggtcttgtc gaatttggcc atgtggccgc cgatagtggg ctcgcgttcg
     1081 acgaggtaga ccttcttgcc cgcgtccgcc atcgtgagcg cggcgtggat gccggcgatg
     1141 ccgccgccga cgatcatcac gtcggggtgc acgggcacgc ggttgcgctg gagcgggacg
     1201 tggcggacga tgcgggtgac cgcccccgcg atgagcgctt tcgccttctc cgtcgccatc
     1261 gccgtatcca gcgtcaccca gctcacgtgt tcgcggatgt tggccatctg gaacatgaag
     1321 gcgttgccgc cccccaccgc cgtcgccttg cggaaagtgt tctcgtgcat gagcggcgag
     1381 cacgaggcca ccaccacccg gttcaccttg ccctcgcgga tatcctgctg gatgatctcc
     1441 tgccccgggt ccgagcacat gaacttgtac tccttcgcca ccgtgacgta cgggagcgag
     1501 gcggcgaaat cggtacaggc tttcacgtcc accgtgtggg cgatgttgat cccgcagtgg
     1561 cagacgtaga cgccgacttt gggcgtgccg ttcgaggact ggtcagccga catgggcgcc
     1621 tccttccacc gcggccgcgg gttcgcggta cgcaaacggg acaaacagcc gctgcagccc
     1681 gagcgggcgc cggtcgagcc cgaacgccgt ccccatgagc tgcgagaagt agacgaccgg
     1741 gacgttcacc cgcgattcgt acatccggct catcttcggc tggtagcact cgaggttgaa
     1801 ctggcagagc gggcaggcgg tggcaatcac gtcgcagccg cgcttctgcg cctcgcgcag
     1861 cagaacgtag ctcagccgca gtccgacctc cggcaccgtg ccggtgagcg agccgccgca
     1921 gcagcgcgtc ttcagcggcc aagccacggg cgtgccgccg agcgccgtga taagccggtc
     1981 catcaccatc ggctcatggg cgtcgtcgaa gtcggcataa ggccgcacga gctggcaccc
     2041 gtagtagcag gccaccttca gcccggccag cggccgggtc accgctccct tgatcgcctc
     2101 caatccgaca tcgttggcga gcacgtcgag cgggtggcgc accttgatcc cgttctcgta
     2161 gctcaggccg gcggcggcga gcgcgctctg gatggtcttg cgtatgtcgg ggtgctcgtc
     2221 gagatagcgg tgggccttgt tcaggccgag gtagcaggcc gcgcagggca cgacgagatg
     2281 cgtgtcgctg tctccctggc gctcggcgat cgcgaaattg cgcgccgaga gcgcaaaggc
     2341 cttcatctcc gaaatcgaca tgtacgccgt cgccccgcag cagttccagt cgtccagctc
     2401 ctcgagcggc cgccccagag cggagaacac ggcgaggatc gattcctcgt aggacttgcc
     2461 ggtgctcttc agcgagcacc cggggtagta gaggtatttc atgcggtcgc ctccgttgtc
     2521 tccgtccggc ggaccatgcg ctcctgctca atcgcggcga gcatggtgtg gagctcgccg
     2581 gttcgcttga tcgactcaag cttgggcgag aggcgtcccc gccggaacag gccgagcccg
     2641 agcccggtgt tgccgaacat cttgaacggg ttggtcctca gaaagagccg caccagcagc
     2701 ggcccctcgt tgctgcggcc cttgcgctcc accccgcgga aaaactcggt cgcgagcacc
     2761 tggctgggaa aacgcttcgg gtgcatgccg ccgcgaatcg ccagccgctt ggccgcatac
     2821 ataatctcgg tgatcttgat ctcctgagga cattcgaccg tacaggcgta acacgaggcg
     2881 cacagccagg tcgtgtaact cgacagcacc tcgccgcgaa agcccgcacg gatcatcgcg
     2941 acaatctgac ggggggtgta atccatgtag gaactcagcg ggcacatgcc gctgcatgtt
     3001 ccgcactgga tacagcggaa gagcttgtcg ccgcccggaa tcgaggcgat ttcgtcggcg
     3061 aa
//
LOCUS       DAOOKS010000177         3053 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_38993_length_3053_cov_3.924616, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000177 DAOOKS010000000
VERSION     DAOOKS010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38993_length_3053_cov_3.924616"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..154)
                     /locus_tag="PK112_03725"
     CDS             complement(<1..154)
                     /locus_tag="PK112_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPC11192.1"
                     /translation="MTHRPQTVLVTGGAGYIGSVLVRVLLRAGLRVRTIDNLSWGGEA
                     LLGVLGD"
     gene            complement(165..2363)
                     /locus_tag="PK112_03730"
     CDS             complement(165..2363)
                     /locus_tag="PK112_03730"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPC11193.1"
                     /translation="MPRFLTATLLSALLLAALGCSESPQEKKQRLFDEVIAAANACDF
                     PAAQKSLQGLTDLEPTAPSARFASGYILERQLQHYDALGVYVALTNTFPSFTDAHAGA
                     WRLYTRLGLLPEALREAEALRELLPHDAEAAVILALALIDSNLPRRAHPFLDSAAAEG
                     ADPARVAMIRARAFIAADQPNAADSAYEAALVGAGSSPEVYLQAASYLETAGLIDSAV
                     TLSRKAVDLSRGDVPTLQQHFELAVRHNYFAEADRVLARLRGAKIPETVVSMLEAFRY
                     DAARRAMPAGEAIDRVIGLGGGQLSMRMYESAIKTKYSNEIVLLHNIQTVSTFLINEG
                     YDREFQEAVFYLLALQAADWLRGKEALVQWEQIRPMYWSRPHIAARHAWALFESGQFD
                     IFATEVEQIHRLHSSQPEWLAELGDVYADIILHQYDTAAALYERALELNPWHRPAFTG
                     WVGMLRRLDRFDEAAKLFDRYPHFPERFPALAMLEGICLAEAGQPERGLEVYLMNAPQ
                     NLGDEAMFQNFFEVLTFRAYREPMRRAAEWIMETGAEHPHILALAAQLRLELGDYPGA
                     IAAADAALKVLPDLLDAKAYRARALCGTGDVDGAIATLRANLDRDQFHVPSTYWLSRI
                     LAEQGREPAQAQDFARRALFDSNQGIREWLNLSYVYQVTGRYDLARGEAIKMTRSFAG
                     EPGPLFRLGMALYLENKPDEARLRLQEAIDRGLAGDDLAEARRVLSTLKS"
     gene            2571..>3053
                     /locus_tag="PK112_03735"
     CDS             2571..>3053
                     /locus_tag="PK112_03735"
                     /inference="COORDINATES: protein motif:HMM:NF025743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HPC11194.1"
                     /translation="MARIQKPPMGRLFVSIIYSSMDALADGLRALERKFGRVQYETVE
                     IACSDRELYREEMGENLQRRLFSFERLFPRDALPEIKGVCHRVEPQFADVVGDFVFRT
                     VNIDPGIITPDQVATASHREFSHRLYLREGVFAQVELIWSHGQFHRLPWTKPDYCEGE
                     A"
BASE COUNT          500 a          943 c         1089 g          521 t
ORIGIN      
        1 gatcgccgag cactcccagg agagcttcgc cgccccagct caggttgtcg atggtgcgga
       61 cgcgcaggcc ggcccgcagg agcacgcgga cgaggacgga tccgatgtag ccggccccgc
      121 cggtgacgag gactgtctgc ggacggtgcg tcatgcggcg ccgcctatga tttcagcgtg
      181 ctgagcacgc ggcgcgcctc ggcgagatca tcgcccgcca gtccccggtc gatggcttcc
      241 tgcagtcgca gccgggcctc gtcgggcttg ttctcaaggt agagcgccat acccagccgg
      301 aacagcgggc cgggctcgcc ggcaaacgac cgcgtcatct tgatcgcctc gccgcgggcg
      361 aggtcgtacc ggccggtcac ctggtacacg tagctgaggt tgagccactc gcggataccc
      421 tggttggagt cgaagagggc gcggcgggcg aaatcctgcg cctgcgccgg ttcgcgcccc
      481 tgctcggcca ggatgcgcga gagccagtag gtcgagggga catggaattg gtcgcggtcg
      541 agattggcgc ggagagtggc gattgcaccg tccacgtcgc ccgtgccgca gagggcgcgg
      601 gcgcggtagg ccttggcgtc cagcaggtcc gggagaactt tcagcgcggc atcggccgcc
      661 gcgattgccc ccggatagtc gccgagctcc agtcgcagtt gggcggccag ggccagaatg
      721 tgggggtgct cggcgccggt ttccatgatc cattcggccg cgcggcgcat cggctcgcgg
      781 taggcgcgga aagtcaggac ctcgaagaag ttctggaaca tcgcttcgtc gccgagattc
      841 tgcggggcat tcatcaggta cacctcgagc ccgcgctcgg gctgcccggc ttccgccagg
      901 catatcccct ccagcatggc gagggcgggg aagcgctcgg ggaagtgggg gtagcggtcg
      961 aacagcttcg cggcttcgtc gaatcggtca agccggcgga gcatgccgac ccatccggtg
     1021 aatgccggcc ggtgccaggg attgagttcg agcgcccgtt cgtacagggc ggccgcggtg
     1081 tcgtactggt gcaggatgat gtcggcgtag acgtcgccga gttccgcgag ccactccggc
     1141 tggctgctgt ggagccggtg aatctgctcg acctcggttg cgaagatgtc gaattgcccc
     1201 gattcgaaca gcgcccaggc gtgccgggcg gcgatgtggg ggcggctcca gtacattggc
     1261 cggatctgct cccactgcac gagcgcctct ttgccgcgga gccagtcagc cgcctgcagc
     1321 gcgaggaggt agaagaccgc ttcctgaaat tcccggtcat acccctcgtt gatcaggaag
     1381 gtcgagacgg tctggatgtt gtggaggagg acgatctcgt ttgagtattt cgtcttgatc
     1441 gccgactcgt acatgcgcat gctcagttgt ccgccgccga ggccgatgac ccggtcgatc
     1501 gcctcgccgg ccggcatggc tctgcgggcg gcatcatagc ggaacgcctc gagcatcgag
     1561 accacggtct cggggatctt cgccccgcgg agccgggcga gcacccggtc ggcctcggcg
     1621 aagtagttgt gccgaacggc cagttcgaag tgctgctgga gagtcgggac gtctcctctg
     1681 gagaggtcga cagctttgcg gctgagggtg acggccgagt cgatcaggcc ggcggtttcc
     1741 aggtacgagg cggcctggag gtagacctcg ggggaggatc cggctccgac cagggcggcc
     1801 tcataagccg agtcggcggc gttgggctgg tcggcggcga tgaacgcgcg ggcccgaatc
     1861 atagctaccc gggccgggtc ggctccctcg gcggcggccg aatccaaaaa cgggtgcgcc
     1921 cgccgcggaa ggttgctatc gatgagggcc agggccagga tcaccgccgc ctcggcgtca
     1981 tgcggcagca attcgcggag ggcctccgcc tcccgcagag cttccggcag cagtccgagc
     2041 cgggtgtaga gacgccaggc gccggcgtga gcgtccgtga aggagggaaa agtgttggtc
     2101 agggcgacat agacgccgag cgcatcgtag tgctggagct ggcgctccaa aatatagccg
     2161 gaggcgaagc gggccgaggg cgcggtgggc tcgagatccg taaggccctg cagcgatttc
     2221 tgcgccgccg ggaaatcgca ggcgttggcg gccgcaatca cctcgtcgaa caaccgctgc
     2281 tttttctcct gcggcgactc ggagcagccg agagccgcca ggagaagcgc ggacaggagg
     2341 gttgcggtga ggaatcgggg catagtctgt ctttctggta acgtggcagt tttcatccga
     2401 ccgggtccga acaaaccgga atttacacct ttttacccgg ccgccaacaa ttatttcccg
     2461 gcggggccgg cgggtgaggg aatcggcgcg cggcgcgggg ggcgccgcgg cggagggcgg
     2521 ggcggatttt tttggttgat tttccaatcg ccgcggcaaa atagtacggc atggcgcgta
     2581 tacagaaacc cccgatgggg cggctcttcg tctcgatcat ctactcctcc atggatgccc
     2641 tggcggacgg gctgcgggct ttggaacgca agttcgggcg cgtgcaatac gaaaccgtcg
     2701 agatcgcctg ctccgaccgc gaactctacc gcgaggaaat gggcgagaat ctccagcgcc
     2761 gtctcttctc gtttgaacgg ctcttccccc gcgacgcgct tccggagatc aagggggtgt
     2821 gccaccgggt ggagccgcag ttcgccgacg tcgtcggcga cttcgtattc cgcaccgtca
     2881 atatcgatcc gggcattatc acccccgacc aggtggcgac cgcctcgcac cgcgagttca
     2941 gccaccggct ctacctgcgg gaaggcgtat tcgcgcaggt cgagctgatc tggtcgcatg
     3001 gccaattcca ccgcctcccc tggaccaaac cggattactg cgagggggag gcg
//
LOCUS       DAOOKS010000178         3050 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_39059_length_3050_cov_3.423706, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000178 DAOOKS010000000
VERSION     DAOOKS010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39059_length_3050_cov_3.423706"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="PK112_03740"
     CDS             complement(<1..275)
                     /locus_tag="PK112_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11195.1"
                     /translation="MAKQAKTTASQPATTETRWPFGKRNYIMLAVALAVIIIGYIALG
                     MGSITLAPLLLVVGYCVLIPVALIIKDPSRTRAAGSDAPSSPFSEKPA"
     gene            complement(347..1615)
                     /locus_tag="PK112_03745"
     CDS             complement(347..1615)
                     /locus_tag="PK112_03745"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPC11196.1"
                     /translation="MSAPATTLADRVLFGLFALFLLSSSFSIALAQSSLGLALAVYIG
                     ILAVRRENPFGGALRPVYLAIGLYVLWVLISALAGPTPGRSVLREREDWLFLIIPIAV
                     HLVRIEPFRRRLVRTLAIAVLLMGAYGVVQHFTGLNWPHATPPLKALEGGYIVQGSFA
                     HRLTYGNYFAVASIFVLACGILAAGRETGVWRRFLFLAAFVGLAATVLSYARMALAGL
                     PLALLFLAALKGRRWLAAAAGAVAIGAVAVYLWVPGVAGGFRAALDRDLTGENQASRT
                     YIWEKSLAIVAENPITGVGLGNFYEAYARQNDSLRGENRVWPHAHNDVLNVAAVAGIP
                     GALLFVAMWVIVFWYLRRGWQRFRGDPAYRALIGAAMAASVMFALCSLSEAAFADEEP
                     RQLLMGVWGMGLGWVNRPSEETPTAAGSAE"
     gene            complement(1612..2202)
                     /locus_tag="PK112_03750"
     CDS             complement(1612..2202)
                     /locus_tag="PK112_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012676120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HPC11197.1"
                     /translation="MKRENRLAELRDLAAQTARLRLEVVEQLGPLLLDLAARVAGVIG
                     AGGKLLIAGNGGSAADAAHFAAEMVVRLTAARSRQALPALALTADTSVLTAAGNDFGF
                     ENVFARQVEGLGHKGDMLLVISTSGNSENLVRAVGAARQRRMLTAGLLGGQGGRLAGL
                     VDPALIVPSASTQRIQEEHIFLIHLLVEYIESDLFG"
     gene            complement(2199..2966)
                     /locus_tag="PK112_03755"
     CDS             complement(2199..2966)
                     /locus_tag="PK112_03755"
                     /inference="COORDINATES: protein motif:HMM:NF003198.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HPC11198.1"
                     /translation="MVEGKVYRRLMEIRADRGGVFLLLIDPDRTSARHNRRLCEAAAE
                     CGVDALLVGTSFMLDTHFAEAVREVKQAAALPVIIFPGSFAQLTPDADAVLFTSLISG
                     RNPEYLIGEQARGAPLVKRFGLEAIPTGYLLIESNNLTSVQYISGTLPIPAAKADIAC
                     AHALAAQYLGMRLVYLDAGSGAHEAVPCAMVREVSAYVEIPVIAGGGLCRPEDCAARI
                     EAGAAMVVVGNSIETDPSFGRLREMAAAVHAKESVRS"
BASE COUNT          524 a         1098 c         1005 g          423 t
ORIGIN      
        1 gccggcttct cgctgaatgg ggatgagggg gcgtcgctgc cggccgcgcg ggtccgcgac
       61 ggatccttaa tgatcagcgc gaccgggatc aggacgcagt agccgacaac gagtagcagg
      121 ggcgccagcg tgatcgagcc catgccgaga gcgatgtagc cgatgatgat cacggccagg
      181 gccaccgcca gcatgatgta gttgcgcttg ccgaacggcc aacgggtctc ggtcgtcgcc
      241 ggctgcgatg ccgttgtttt tgcttgtttt gccataacct gtcgacttcc aataagatcg
      301 ccggacctgt tcctgtcaag cggttttccg gggcggcccc gcccgctcac tccgccgaac
      361 ccgccgccgt cggcgtctcc tcggacggcc ggttgaccca gcccagtccc atcccccaga
      421 cgcccatcag caactggcgg ggctcctcat cggcaaacgc cgcctccgac agcgagcaga
      481 gggcaaacat gaccgacgcc gccatcgccg cgccgatcag cgcgcggtaa gcgggatcgc
      541 cccggaaccg ctgccagccg cgccggagat accaaaacac gatgacccac atggcgacaa
      601 aaaggagggc gccgggaatt ccggccacgg ccgcgacgtt gaggacgtcg ttgtgcgcat
      661 gcggccacac ccgattctca ccgcgcaaag aatcgttctg ccgcgcatag gcctcgtaga
      721 agttgcccag gccgaccccg gtgatcgggt tctccgctac gatcgcgagc gatttctccc
      781 agatgtacgt gcgcgacgcc tggttctcgc cggtcaggtc ccgatccagc gccgcccgga
      841 accccccggc caccccgggc acccagaggt acacggcgac ggcgccgatc gccactgcgc
      901 ccgcggccgc cgccagccac cgccgccctt tgagggccgc gaggaacagc agggcgagcg
      961 gcagcccggc aagcgccatc cgcgcatagg agagcacggt cgccgccagg cccacgaagg
     1021 ccgcgaggaa aagaaatcgc cgccacaccc cggtctcccg cccggcggcc agtattccgc
     1081 aggccagcac gaagattgag gccaccgcga aatagttgcc gtaggtgagg cggtgcgcaa
     1141 atgacccctg cacgatgtac cctccctcga gcgctttgag cggcggcgtc gcgtgcggcc
     1201 agttgagccc ggtgaagtgc tgcacgacgc cgtacgcgcc catcagcagc acggcgatgg
     1261 cgagcgtccg caccagccgc cggcggaacg gctcgatgcg cacgaggtgg acggcgatcg
     1321 gaatgatgag gaagagccag tcctcccgct cccgcagcac cgaccggccg ggggtcggtc
     1381 ccgccagcgc cgagatcagc acccagagaa catacagccc gatcgccagg tagaccggcc
     1441 ggagcgcgcc gccgaacgga ttttcccggc gcacggcgag gatgccgata tagacggcca
     1501 gcgccaggcc gaggctgctc tgggccagcg cgatcgagaa acttgaactc agaaggaaga
     1561 gcgcgaacaa gccgaagagc acgcggtcgg cgagtgtggt cgccggcgcg ctcatccgaa
     1621 caggtcgctt tctatgtact cgacgagcag gtggatgagg aagatatgct cctcctggat
     1681 ccgctgggtc gacgcgctcg gcacgatcag ggcagggtcc accagtccgg ccagccgtcc
     1741 cccctggccg ccgagcagcc ccgcggtcag cattctccgt tggcgcgcag cgccgactgc
     1801 ccgcacgaga ttctccgaat tgcccgaggt cgagatcacc aggagcatgt ctcccttgtg
     1861 gcctagccct tccacctggc gggcgaacac gttctcgaag ccgaaatcgt tccccgcggc
     1921 cgtcaggacc gatgtgtccg cggtgagcgc gagcgccggc agggcctggc ggctccgggc
     1981 cgccgtcaat cggaccacca tctcggccgc gaagtgcgcg gcatcggccg ccgagccgcc
     2041 gttcccggcg atcagcagct tgccccccgc gccgatcacg ccggcgacgc gggcggccag
     2101 gtcgagcagg agggggccga gttgttcgac gacctcaagg cggagccgcg cggtctgggc
     2161 ggccaggtcg cgcagttcgg ccagacgatt ttcccgtttc atgatcgcac ggactccttc
     2221 gcatgcaccg ccgccgccat ctcccgcagg cgcccgaacg acgggtcggt ttcaatcgaa
     2281 tttcccacca ccaccattgc cgcgcccgcc tcgatccgcg ccgcgcagtc ctccggccgg
     2341 cacagcccgc cccccgcgat caccgggatc tcgacatagg cgctcacctc gcggaccatc
     2401 gcgcacggca ccgcctcgtg cgccccgctc ccggcgtcga gatagaccag ccgcatgccg
     2461 aggtactggg ccgccagcgc gtgcgcgcac gcgatatcgg ccttggcggc cgggatcggc
     2521 agcgtccccg agatgtactg caccgaggtc aggttgttcg attcgatgag caggtagccg
     2581 gtggggatgg cctcgaggcc gaagcgcttg accagcggcg ccccgcgcgc ctgctccccg
     2641 atcagatact ccgggttgcg gccggagatg agcgaggtga acagcaccgc gtccgcatcg
     2701 ggcgtcagtt gcgcaaacga cccggggaaa atgatcaccg ggagcgcggc cgcctgtttg
     2761 acctcgcgga ccgcctcggc gaaatgggtg tcgagcatga acgaggtccc caccagcagg
     2821 gcgtcgacgc cgcactcggc ggccgcctcg cagagccggc ggttgtgccg cgccgaggtg
     2881 cggtccgggt cgatcagcag gagaaacacc ccgccgcggt cggcccggat ctccataagc
     2941 ctgcggtata ctttgccttc aaccatctcg tgtccctaag ccggcgcgcc cgcggtgagg
     3001 gccgctaccg gcgctgcgcc gcggtgaggc tcgcccgcac cgccgcgaac
//
LOCUS       DAOOKS010000179         3031 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_39398_length_3031_cov_2.686492, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000179 DAOOKS010000000
VERSION     DAOOKS010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39398_length_3031_cov_2.686492"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="PK112_03760"
     CDS             <1..186
                     /locus_tag="PK112_03760"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HPC11199.1"
                     /translation="FAYQGIAGAEYRFNQHWSAFGEYRYVGMTDPEVTLFPGAGGVTR
                     DLDVDSHNVGGGIRYRF"
     gene            complement(614..2071)
                     /locus_tag="PK112_03765"
     CDS             complement(614..2071)
                     /locus_tag="PK112_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007225937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HPC11200.1"
                     /translation="MPPYVRHRPERTLLYQLVRDYYPAFKAHLAAQGSTLPPYVEREF
                     EDYLECGRLEHGFLRVRCDSCHAEHLVAFSCKRRGFCPSCGARRMAESAALLVDEVFP
                     EQPVRQWVLSFPFPLRFLFASRPEVMTGVLGIVYRCIATHLIKKAGFSRKTAQAGAVT
                     LIQRFGSALNLNVHFHMLFLDGVYVERPDGSLRFRWVRAPTSAELAGLTKTLARRIGR
                     HLEHQGLLERDAENSYLADDELELGAMAQLQSSSITYRIAVGPQQGRKVFTLQTLPAC
                     EEPFDTGVGKVAGFSLHAGVAARADQREKLERLCRYISRPAISERRLSLTPNGNVRYQ
                     LKTPYRDGTTHVIFEALDFIARLAALVPKPRVNLTRFHGVFAPNSKYRARVTPAKRGR
                     GGQPATTVDPEERTPAERRAAMRWAQRLKRVFGIDIETCAACGGSMRIIACIEEPDVI
                     EKILAHLDAKVAEPDAPRRPPCRAPPQRRLFDETG"
     gene            2696..2821
                     /locus_tag="PK112_03770"
                     /pseudo
     CDS             2696..2821
                     /locus_tag="PK112_03770"
                     /inference="COORDINATES: protein motif:HMM:NF013260.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            2939..>3031
                     /locus_tag="PK112_03775"
     CDS             2939..>3031
                     /locus_tag="PK112_03775"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HPC11201.1"
                     /translation="MQRAQRRIGADLHDGLGQELTGIAMMLRSLQ"
BASE COUNT          509 a         1056 c          944 g          522 t
ORIGIN      
        1 tttgcctacc agggtatcgc cggtgcggaa taccgcttca accagcactg gtccgcgttc
       61 ggtgagtatc gttatgtcgg tatgaccgat cccgaggtca cgctgttccc gggcgccggc
      121 ggggttaccc gtgacctcga cgtcgactcg cacaacgtcg gcggcggtat ccgctaccgc
      181 ttctgagcct gacggtaccc gggtggtgcg ccacccgggt atctgtcgcg gacaccgttt
      241 ccgcacgctg acctgaaacc tccacgcatc gcgtcggccc tgccgcctca cggcagggcg
      301 cggcgccttt ggccgttatc cccgccggcg ttctaccctc tcgattccca ctgctgcgga
      361 ttcagagtat aggaagaata aaccaccgtt tcgtgtgctc gcagcgggca gccttgcctg
      421 attgacggcg gggtctaccg tcggcaagcc gcttctgctc agccatcggg gccaagactg
      481 gcgtcacaca gccgaattct tctcgacgac ggcagcgccg ccaccctttt tctctcccta
      541 ccggtttccc gaccagccgc cgccatggcc gtgccgctga cggcaaagcc ctaaagaaac
      601 catcctgggg ttgtcacccc gtctcgtcga acagccgccg ctgcggcggc gcccggcaag
      661 gcggccgccg cggggcgtcg ggctcagcca ctttcgcatc caggtgagcg agtatcttct
      721 cgatgacgtc gggttcctca atgcaggcga tgatccgcat cgacccgccg caggccgcgc
      781 aggtctcgat gtcgatcccg aacacccgct tgaggcgctg cgcccaacgc atcgcggccc
      841 gacgctcggc cggtgtccgc tcctccggat ccacagtcgt ggcaggctgg cctcccctgc
      901 ctcgcttggc cggtgtcacc cgcgcccggt acttgctgtt gggagcgaat actccgtgga
      961 agcgggtcag gttgacccgc ggcttgggca ccagggccgc caaccgggcg atgaaatcca
     1021 atgcctcgaa gatgacgtgc gtcgtgccat cgcggtacgg cgtcttcaac tggtaccgga
     1081 cgttgccatt cggtgtgagc gacaggcgcc tttccgagat cgccggccgg ctgatatacc
     1141 ggcacaaccg ctcgagcttc tcgcgttgat ccgcccgcgc cgccacaccg gcgtgcaggg
     1201 agaacccggc aaccttgccg accccggtat cgaacggctc ctcgcacgcc ggcagcgtct
     1261 gcagcgtgaa caccttgcga ccctgctgcg gcccgacagc aatgcggtag gtgatcgacg
     1321 aactctgcag ctgagccatc gccccgagct cgagttcgtc atcggccagg tagctgttct
     1381 ccgcatcgcg ttccaacaag ccctggtgct cgaggtggcg gccgatgcgc cgtgccaggg
     1441 tcttagtaag cccggccaac tcggcgctgg tcggggccct cacccagcgg aagcgcagcg
     1501 agccatccgg acgctcgacg tacacaccgt cgaggaacag catgtggaag tggacgttga
     1561 ggttgagcgc gctgccgaag cgctggatca aagtgacggc gccggcctgg gccgtcttac
     1621 gcgagaaccc cgccttcttg atcaggtgcg tggcgatgca gcggtagacg ataccgagca
     1681 caccggtcat gacctctggg cggctcgcga acaggaagcg caacggaaac gggaagctca
     1741 acacccactg gcgcaccggc tgctcgggaa agacctcatc gaccagcagc gccgcgctct
     1801 ccgccatgcg ccgggcgccg cagctcgggc agaagccgcg ccgcttgcag ctgaacgcga
     1861 ccaggtgctc ggcgtggcaa ctatcgcaac gcacccgcag gaagccatgc tcgagacgac
     1921 cgcactcgag gtagtcttca aactcccgct cgacataggg aggcaacgtc gaaccctgcg
     1981 ccgccagatg cgccttgaac gccgggtagt agtcccgaac gagttggtag aggagcgtgc
     2041 gctcgggacg gtggcgcaca tacggcggca cgccgacacc cgaactggcg tccctgccag
     2101 ccggcaatgg ctccatgctc gcgtccttgc gagattggcc tcattcgaag cgtagcaggc
     2161 ccgaaacacc gagtttgact attgcgcacc cggccaggga gcccgatagg agcgcaatcc
     2221 gttgcactat gtgatttcag ttgcaatgca acatcgagac gtacaaccca cgcaggcctc
     2281 aattgcgtac cgcacgcgtg cggtccgcgc cgtagatgac gtctctgcgt accgccaggt
     2341 cccctcatcc accccggcct ggccggccgc catccgggcc gtccatccca tctggcgacg
     2401 cttggtcttc atccgctcac cgagggtcgt gggcgttgga ttcgggtcac ctcccaggaa
     2461 gtcgatcaga gccggccagt tgcggatgag gggctcgcgt cggtcctttt cccagttgag
     2521 atacgtaaat tcgtcgacac caaggagctt cgccgcctct ttttggtgca gttgtcggat
     2581 cgagcgctcg cgaagcagat gttcgcccag ggtttgcgga tttttcggat aagtcgttgg
     2641 tttttcggct gttcgccgca cccggacgcc tgatggcaaa agagcaacac atccctgtcc
     2701 ctgcggctac gccggcgacg cccagatcgc ctgccgctgc acgccggagt gtgcccaggc
     2761 ctaccgcgcg cgcatctccg gaccactcgc cgaacgcatc gacctgcacg tcgacgtgcc
     2821 gcgccagacc acctccctgc tcgatgcggc gccggcggcg gccgagtcga gcacggctgt
     2881 tcgcgcacgg gtcgcgcgcg cgctgcagcg ccaggtcgag cgccagatca tcgacgcggt
     2941 gcagcgcgcc cagcggcgca tcggcgccga cctgcacgac ggcctcggcc aggagctcac
     3001 cggcatcgcg atgatgctgc gcagcctgca g
//
LOCUS       DAOOKS010000180         3027 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_39462_length_3027_cov_3.359354, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000180 DAOOKS010000000
VERSION     DAOOKS010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39462_length_3027_cov_3.359354"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="PK112_03780"
     CDS             complement(<1..477)
                     /locus_tag="PK112_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015906016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPC11202.1"
                     /translation="MADVRTTHRGPVGYSTYSDQMIYDFESSYGAHHYSRQHLVVRKA
                     EGIWLHDEKGDRYLDCPAAYSAVNQGHHHPRIVQAVVEALQSGYGSVISNVVYTDPLG
                     VFLKKLATLVPQMGPRFGDNGNKVLPKNGGVESVETAVKLARYYGYKEKGIPDGQQE"
     gene            882..1760
                     /locus_tag="PK112_03785"
     CDS             882..1760
                     /locus_tag="PK112_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HPC11203.1"
                     /translation="MPKIVDNVELGPMIWKMRVAVPRLVAKAKAGQFVILRVNNEGER
                     VPMSIAGLDQANGLLTIVYQVVGKTSALMTTVAPGHELSDCVGPLGIPTHVDTWGTAC
                     VVGGGIGIAPVYPIAQAFKKAGNKVVTIIGARTRDLLFYEEEHKAVADEFYPCTDDGS
                     YGHHGFVSDVLKQLLDRGAPIKHIMAIGPVPMMRVVSKLTAPYGVSTWVSLNPLMIDG
                     TGMCGGCRVKIGEETKFACVDGPDFDGHLVDFDLLTSRLTAYREQERRAMKRLLDDPN
                     CRLTKSVQAFAYPDEK"
     gene            1855..>3027
                     /gene="gltA"
                     /locus_tag="PK112_03790"
     CDS             1855..>3027
                     /gene="gltA"
                     /locus_tag="PK112_03790"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014959028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HPC11204.1"
                     /translation="MRIPRQKMPEQQAEVRVGNFKEVPFGFTIDLALTEAQRCLECPK
                     APCIKGCPVEVDIPGFIRLILEKDFAAAARKIKETNSLPAVCGRVCPQEEQCEIACVL
                     HKKFQPVAIGALERFVADYERENHLVQLPRKQPPTGRKAAVVGAGPAGLTVAGDLILL
                     GHEVTIFEALHKPGGVLVYGIPEFRLPKAIVQSECDYLRDLGVEFRNSTVIGKMDTID
                     ELFEMGYDAVFIGTGAGLPNFMGVPGENLIGIYSANEYLTRSNLMRAFEEDSYDTPII
                     RGRNVAVLGGGNTAMDAVRTALRLGAENAYIVYRRSEAEMPARKEEIHHALEEGVQFH
                     MLTTPIRFLGDERNRVRAMECLQMELGEPDASGRRRPVPIEGSNFTMEVDLVVVAIG"
BASE COUNT          588 a         1015 c          883 g          541 t
ORIGIN      
        1 ctcctgctgc ccgtccggaa tccccttctc cttgtagccg tagtaccggg ccagcttgac
       61 ggccgtctcc accgattcga ccccgccgtt tttcggcagc accttgttgc cgttgtcgcc
      121 gaaacgcggg cccatctgcg gcaccagcgt cgccagcttc ttgaggaaca cgcccagggg
      181 atcggtatag acgacgttgg aaatgaccga gccgtaaccg ctctggagcg cctcaacgac
      241 cgcctgaaca atacgcggat ggtggtggcc ctggttcacg gccgagtagg ccgccgggca
      301 atcgaggtac cggtcgccct tctcatcgtg caaccagatc ccctcggcct tccggaccac
      361 cagatgctga cgactgtaat ggtgtgcacc gtaactgctc tcgaaatcgt agatcatctg
      421 gtcagagtac gtcgagtacc caaccggtcc gcggtgagtc gttcttacat cagccatggt
      481 acacctcgta aaaaagccat aaaaaatggc gttacacgtc gcccaaatat atgtcgtccg
      541 ccccaaaaac tcaagtgatg ccgctcgaaa aacgcgcatt ctgcgttgta gttcagtcac
      601 tttcgcgcct gtttccggcc cgctccgcag cgtgatttca ttcggggaat cgtcgaaaat
      661 agaccagatc ggggctattt ggtccctccg tcgcgcgtcg gaccacccgt tccgggtcgg
      721 tcgggaagga catgtgcccc aagcgaatgt gcgcgaaaaa aaatacccga tcgtaccggt
      781 tttgctcgac ctaacaggag taaaattgac cggataagct tgatttttcg gccccaattg
      841 gtatattctt cggctgattc taccaccact ttggaggtct gatgcctaag attgtcgaca
      901 atgttgaact cggcccgatg atctggaaaa tgcgcgtggc tgtcccgcgc ctcgtcgcga
      961 aagccaaagc cggccaattc gtcatcctgc gcgtcaacaa cgagggggag cgcgtgccga
     1021 tgtcgatcgc cggcctcgac caagccaacg gactcctcac catcgtctac caggtcgtgg
     1081 ggaagacctc ggcgttgatg acgaccgttg cgccgggcca cgagctgtcc gattgcgtcg
     1141 ggccgctcgg catccccacc cacgtcgata cctggggcac cgcctgcgtc gtcgggggcg
     1201 gcatcggcat cgcgccggtc tacccgatcg cccaggcctt caaaaaagcc ggcaacaaag
     1261 tggtcacgat catcggcgcc cgcaccaggg acctgctttt ttacgaagag gagcacaagg
     1321 ccgtggccga tgagttctac ccgtgcacgg acgacggatc ctatggccac catggttttg
     1381 tctccgacgt tctcaagcag cttctcgacc gcggcgcccc gatcaagcac atcatggcga
     1441 tcggcccggt gccgatgatg cgcgtcgtgt caaagctgac ggcgccctac ggcgtctcga
     1501 cctgggtgtc gctgaacccg ctgatgatcg acggcacggg catgtgcggc gggtgccgcg
     1561 tgaaaatcgg cgaggaaacc aagtttgcct gcgtcgatgg tccggatttc gacggccacc
     1621 tggtcgattt cgacctgctc acctcccgcc tgacggccta ccgcgagcag gagcggcggg
     1681 cgatgaaacg gctgctcgac gacccgaact gccgcctgac caagtccgtc caggccttcg
     1741 cctacccgga tgagaagtga ggaaacgacc atgagcgaaa aagacaccgc cctggggcag
     1801 gatgaatccc gggccgaggc cggctcctcg aatccgatcg ccaagaaaga ccgcatgcgc
     1861 atcccgcgcc agaagatgcc ggaacagcag gcggaggtgc gggtcggcaa tttcaaagag
     1921 gttcccttcg ggttcacgat tgacctggcg ctcaccgagg cccagcgctg tctcgagtgt
     1981 cccaaagccc cctgcatcaa gggctgcccg gtggaggtcg acatccccgg cttcatccgc
     2041 ctcatcctgg aaaaggactt cgccgccgcc gcccgcaaga tcaaggagac gaactccctg
     2101 cccgccgtct gcggccgcgt ctgcccgcag gaggagcagt gcgaaatcgc ctgcgtgctg
     2161 cacaagaagt tccagccggt cgcgatcggc gccctcgaac gcttcgtcgc cgactacgag
     2221 cgcgagaacc atctcgtcca gcttccccgg aagcagccgc cgacggggcg caaagccgcg
     2281 gtcgtcggcg ccggcccggc cggactcacg gtcgccggcg acctcatcct tctcggccac
     2341 gaggtcacta ttttcgaggc tctccacaag cccggcggcg tgctcgtcta cggcattccg
     2401 gaattccgcc tccccaaggc gatcgtccag tccgagtgcg actacctccg cgacctcggc
     2461 gtcgaattcc gcaattccac cgtcatcggc aaaatggaca ccatcgacga gctcttcgag
     2521 atgggctacg acgcggtttt catcggcact ggcgccggcc tgccgaattt catgggcgtc
     2581 cccggcgaaa acctcatcgg catctactcc gccaacgagt acctcacccg gtcgaacctc
     2641 atgcgggcgt tcgaggagga cagctacgac acgccgatca tccgcggccg caacgtcgcc
     2701 gtcctcggcg gcggcaacac ggccatggat gccgtccgca ccgccctccg gctcggcgcc
     2761 gaaaatgcct acatcgtcta ccgccgcagc gaggccgaaa tgcccgcccg caaggaggaa
     2821 atccaccacg cgctcgaaga gggcgtgcag ttccacatgc tcacgacccc catccggttc
     2881 ctgggcgatg aacggaaccg ggtgcgggcg atggagtgcc tccagatgga gctcggcgaa
     2941 ccggacgcct ccggccggcg ccggcccgtc cccatagaag gttcgaattt caccatggag
     3001 gtcgatctcg tggtcgtggc gatcggc
//
LOCUS       DAOOKS010000181         3026 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_39483_length_3026_cov_2.752609, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000181 DAOOKS010000000
VERSION     DAOOKS010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39483_length_3026_cov_2.752609"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..277)
                     /locus_tag="PK112_03795"
     CDS             complement(23..277)
                     /locus_tag="PK112_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11205.1"
                     /translation="MIKAFVAVIGVIFLMGLLGLGGIGLLLGLIGGLFGLIAGILGAA
                     LGLVVGLFGVVFGTIGGLVGILLPVAAVVVILAGLAYLLA"
     gene            complement(407..889)
                     /locus_tag="PK112_03800"
     CDS             complement(407..889)
                     /locus_tag="PK112_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017324931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HPC11206.1"
                     /translation="MPRRYWLMKSEPNRFSIDDLRAAPQGIAPWDGARNYQARNLLRD
                     RVKAGDGVLFYHSGTATPGIAGIAEVVRAGYPDHTAWEANGEQFDPKSTPARPVWYMV
                     DVRFVEKFRQLIPLAKLKTTPGLEGMEVARRGSRLSVQPVSRSEWEIVRRIARNMDPG
                     "
     gene            1004..1663
                     /locus_tag="PK112_03805"
     CDS             1004..1663
                     /locus_tag="PK112_03805"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010297549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HPC11207.1"
                     /translation="MADRTKEEKYALLRSRMVDEQLVRRGISDPGVLAAMRAVPRHLF
                     IPEKYRADAYRDSPVPIGSGQTISQPYIVASMTEELALDHASTVLEIGTGCGYQTAVL
                     AEIAGRVYSIERLPDLHELACATLGLLRYDRVNLRLGDGYRGWAEEAPFDGIIVTAAA
                     PRIPERLTGQLSVGGRLILPVGSEFGRQELVLIERTATGLVEKVLYEVRFVPMQGDSQ
                     A"
     gene            2052..2450
                     /locus_tag="PK112_03810"
     CDS             2052..2450
                     /locus_tag="PK112_03810"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPC11208.1"
                     /translation="MRMKYIQKQSGLSLLEVLIGMIVLAMGLLGLAPMLVMSIEGNVM
                     SRDNTVAANLAKQQVEQYEGMNSLPAIPFTLQETGLAGGRYTRSTYIRGHATDASIPT
                     GVYQIDVTIAWTDNQNVPRTTQYSTFILEP"
     gene            2470..>3026
                     /locus_tag="PK112_03815"
     CDS             2470..>3026
                     /locus_tag="PK112_03815"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPC11209.1"
                     /translation="MYRPLRHSAGYTIMELLVALVLTGIVAAAGFSFYVSMHNSALTQ
                     EDISNMQHTSRSSLQEIAKTLRMAGYKLAGHAAYRISGDSLYVFFSDTQPVDTVLYYL
                     QENTALGVNAPNGWKPMFLMKKVNNQPAAVFSDVVRSITYTVIDSANVQVSIEAQTAK
                     SDESWDEDSGYRSFTATERVTIRNL"
BASE COUNT          563 a          991 c          953 g          519 t
ORIGIN      
        1 caccgcaatt gtcggacccg cttcaggcca gcaggtaggc caggccggcc aaaatcacaa
       61 ctaccgccgc caccggcaag aggataccga caagcccgcc gatcgtgccg aagacgactc
      121 cgaacaggcc gaccaccagg ccgagagccg cgccgaggat ccccgcgatc aggccgaaca
      181 gcccgccaat caggccgagc aacagcccga tcccgccgag cccaagcaat cccatcagga
      241 agatgactcc gattaccgcc acaaacgcct tgatcacgtc tcgttcctcc tcatccttcg
      301 gaccagccag ccactcttca ctacgttatc cggccgtcga ggttgcatcc gcacggagcc
      361 gccggcgctg ccgtcaacga ggattacgcc cgagcggccg gactattcac ccgggatcca
      421 tgttccgggc gattctccgt acaatctccc actccgatct cgacaccggc tggaccgaca
      481 ggcgggagcc gcggcgagca acctccatgc cttcaagccc gggcgtcgtt ttcaacttcg
      541 ccagcgggat gagctgccga aacttctcca cgaaccgcac atcgaccatg taccagaccg
      601 ggcgcgccgg agtcgatttg ggatcaaatt gctcgccatt ggcctcccag gccgtgtgat
      661 cgggataacc ggcccgcacc acctcagcga tccccgcgat tccgggggtg gccgtgccgc
      721 tgtgatagaa gagaactccg tcaccggctt tcacgcggtc gcggaggaga tttcgggcct
      781 ggtagttgcg ggcaccgtcc cagggagcga tcccctgcgg tgcggcgcga aggtcatcga
      841 tggagaagcg gttcggttcc gatttcatca gccagtatcg gcggggcatc tttttgcgtc
      901 ctttctctgt tgagcgtcca ccgccatcca gtatattgca gccgcctcgg gccggcaagt
      961 cggtcggccc tggcggcggc ttgcgccttc ggagagtgac gcaatggcgg atcgaaccaa
     1021 ggaagagaag tacgcgctgc tgcgcagccg gatggtcgat gagcagctgg tgcggcgcgg
     1081 tatcagcgat cccggggtgc tggccgccat gcgggcggtg ccgcggcacc tgttcattcc
     1141 cgagaagtac cgggccgacg cctaccgcga cagccccgtg ccgatcggct ccgggcagac
     1201 tatctcgcag ccctacatcg tggcctcgat gaccgaggaa ctggcgctcg accacgcaag
     1261 cacggtgctt gaaataggga ccggctgcgg ctaccagacc gctgtcctgg ccgagatcgc
     1321 cggccgcgtc tattcgatcg agcggttgcc cgatctccac gagctggcct gcgccaccct
     1381 cggtctgctc cgctacgacc gcgtgaacct gcgtctcggc gatggttacc ggggctgggc
     1441 ggaggaggcg ccgtttgacg gcatcatcgt caccgcggcc gctccgcgga tcccggagcg
     1501 gttgacgggt cagttgtcgg tcggggggcg gctcattctg ccggtcggat ccgaattcgg
     1561 ccggcaggag ctggtcctca tcgagaggac ggccaccggc ttggtggaga aagtcctcta
     1621 cgaagtgaga tttgtaccga tgcaggggga tagccaggcc tagcggcggt cccgcccccc
     1681 tgggatcttc cgaagtcctg ccgctgcttt tcgtccacac cgacccttca agctaagcct
     1741 cctttgagaa cgtccccttg agccccatgg gcctcgatcg cgactgggcc ggaatgctcc
     1801 cacgatcgcc cggcttctcg gccacgggct tcctgatctc ctgcgctccc gggggatggg
     1861 ctgagctccg gcagagtatt gggaacctcc cccatcttgg ccgggcgcgg ggcggccggg
     1921 gcgataaatc gcccggcggt tcgtattgtc cgccaatagg ttgcagcccg atgagcagtc
     1981 ccgggaacgg gtcggagcgt ttggcagccg ttttgcggtg gtccagggaa aagacaaagg
     2041 agtctgcggc tatgagaatg aaatacattc agaaacagag cggcctgtcg ctgctggaag
     2101 ttctcatcgg catgatcgtg ctggcgatgg gtctgctggg tctcgccccg atgctggtca
     2161 tgtcgattga ggggaacgtg atgtcccggg acaacaccgt ggccgccaac ctggccaagc
     2221 agcaggtgga acagtacgaa gggatgaact cgctcccggc gattccgttt acgctgcagg
     2281 agacgggcct cgccggcggg cggtacaccc gctcgaccta tatccgcggg cacgccaccg
     2341 acgcctccat ccccacgggg gtctaccaga tcgacgtgac gatcgcgtgg acagacaacc
     2401 agaacgtgcc gcgcaccacg cagtactcca cgtttattct cgaaccatga cggggaagga
     2461 cggaaggcaa tgtatagacc acttcgacac agcgcgggct acacgatcat ggaactgctc
     2521 gtggccctgg tgctcacggg cattgtggcg gcggccggtt tcagcttcta cgtctccatg
     2581 cacaactcgg cgctgacgca ggaggacatc tccaacatgc agcatacgtc gcggtcgagc
     2641 ctgcaggaga tcgccaagac gctgcgcatg gccgggtaca agctggccgg gcacgcggcc
     2701 taccggatca gcggcgacag cctgtacgtc ttcttcagcg acacccagcc ggtggacacg
     2761 gtgctgtact acctgcagga gaacaccgcg ctcggggtca acgcgccgaa cgggtggaag
     2821 ccgatgttcc tgatgaagaa ggtgaacaat cagcccgccg cagtcttctc cgatgtcgtg
     2881 cgctcgatta cctacaccgt catcgactcg gccaacgtcc aggtctcgat cgaggcgcag
     2941 acggcgaagt ccgacgagtc gtgggacgag gacagcggct accggtcgtt cacggcgacc
     3001 gagcgggtga cgatccgaaa cctcga
//
LOCUS       DAOOKS010000182         3023 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_39537_length_3023_cov_3.278976, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000182 DAOOKS010000000
VERSION     DAOOKS010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39537_length_3023_cov_3.278976"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="PK112_03820"
     CDS             complement(<1..246)
                     /locus_tag="PK112_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006371254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPC11210.1"
                     /translation="MREFDLVVIGSGPAGEKAAIEAAKMRRSTAIVERRSVQGGVCIH
                     TGTIPSKTLRETVLYISGLRQRSVYGLVGGVRANVSVA"
     gene            complement(249..1310)
                     /gene="rmuC"
                     /locus_tag="PK112_03825"
     CDS             complement(249..1310)
                     /gene="rmuC"
                     /locus_tag="PK112_03825"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HPC11211.1"
                     /translation="MDPALVLLVVLTAMLTAAVVGLAVSLRRRQTAETQTVQAAVDRL
                     RAELVGAHLEGLATLRSSLDAAARGLNERLAEGTSAMDRRLTVVAEIEHRLGELQRQT
                     QNLEDIGRNIQALSDLLKPPKLRGGLGELLLENLLGQILPPALYAMQHGFRDGSRVDA
                     VIRLGDRFLPVDSKFPLDAFARLSAESAGEAAAREFQRAVKVQIDAIAAKYIRPDEQT
                     TDFAVMYVPSEAVYARLISDEGGGLLPYALARRVIPSSPGHLYAFLASVAAIYREAGL
                     AGNTRRLSVVVSGVRGALERLDRTHERMAGSTRMLALNLDKARAEAGAMKELLDRVHG
                     PDAAEPAEDNPAAGEGRIK"
     gene            complement(1322..2908)
                     /locus_tag="PK112_03830"
     CDS             complement(1322..2908)
                     /locus_tag="PK112_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11212.1"
                     /translation="MKGRSFVLAALLVAFRPAVSPAAEPFSTRPAIETLEEYFDLLVS
                     GNLASAEGFWTQAAIERSGRFDITFTDIPLKIDAGSPVVRNLPVMRDYLVPAVTQVDL
                     LDGGRYVRMKFKKTVNGEEVEHPYYAYWDSTYFWLTYPQDWVAALWPVRETRYFRIHT
                     QPGAEAYLHPVLLDEADRFIERAADSLSLGAAVLREIAEKKIEFFYCTSDSTVLTITG
                     HLTKGTYDLASNDIISATFPHNHEVVHLLVNLKLRSLPLMTQPIVREGIAVHLGGRWG
                     KAPTSLVYLGAFLHKNGLVQVDSLVSMDRFEKQAGADMAYPVAGLFTAFLLDRMGYSR
                     YFDLYLKLSGSSTEMPAMLAPEVKRVVYETVGVSSWDNLMMEFSAYCDRLESERAVFA
                     PGLVERKQGGFDDGGVKVRLYQRWVSLSFPANGPDGPRGNLLFGYDKRLKDVKSVLFE
                     EQYQGGTPFPGYRWGIRYDANEAGLYDYATNQLVGKYIYGITPSAAYRGTAQEVQVRF
                     LIDLSEGLAPTAKDHKLLAD"
BASE COUNT          501 a          961 c         1048 g          513 t
ORIGIN      
        1 ggccaccgag acgttggcgc ggacgccgcc gacgagtccg tagacggacc gctggcgcag
       61 gccggagatg tagagcacgg tttcgcggag ggtcttggag gggatggtgc cggtgtggat
      121 gcagacgccc ccctggacgg agcggcgctc gacgatggca gtggaccggc gcatcttggc
      181 cgcctcaatg gcggcttttt cgccggccgg gccggacccg ataacgacca gatcgaattc
      241 gcgcacgatc atttgatcct gccctccccc gccgccgggt tgtcctcggc gggttccgcc
      301 gcatcgggac cgtggacccg gtcgagcaat tctttcatag cgcccgcttc ggcgcgcgcc
      361 ttgtcgagat tgagcgccag catacgggtc gacccggcca ttcgctcgtg cgtccggtcg
      421 aggcgctcga gcgccccccg gaccccgctc acaacaaccg acagccgccg ggtgttccct
      481 gccaggccgg cctcgcggta gatcgcggcc accgaggcca agaaggcgta gaggtggccg
      541 ggggagctgg gaatcacccg tcgcgccagg gcgtagggca gcaggccgcc cccctcgtcg
      601 gagatcagcc gggcgtagac cgcctccgaa ggaacgtaca tgaccgcaaa atcggtcgtc
      661 tgctcgtccg gccgaatgta cttggcggcg atggcgtcaa tctgcacttt gacggcgcgc
      721 tggaattcgc gggccgcggc ctcgcccgcg ctctcggcgg agagacgggc aaaggcgtcc
      781 agcgggaatt tcgaatccac ggggaggaac cggtcgccga ggcggatgac ggcgtccacc
      841 cggctgccgt cgcgaaaccc gtgctgcatg gcatacaggg cggggggcag aatctggccg
      901 agcaggtttt ccaggaggag ttcgcccagg ccgccgcgga gcttgggcgg cttgagcaga
      961 tcggagagcg cctggatatt gcgcccgatg tcctcgaggt tctgcgtttg ccgctggagt
     1021 tcgccgagcc ggtgctcgat ctcggccacc accgtcagcc gccgatccat ggccgaggtt
     1081 ccttcggcga gccgctcgtt gagtccgcgc gccgcggcgt cgagactgga gcgcagggtc
     1141 gccagtccct cgaggtgggc gccgaccagt tcggcccgca gccggtcgac cgccgcctgc
     1201 accgtctgtg tctcggcggt ctgacggcgc cgcagcgata cggccagccc gacgacggcg
     1261 gccgtcagca tcgccgtcag caccaccagg aggacgagcg cgggatccat gccgggtccg
     1321 gtcagtcggc caggagtttg tggtccttgg cggtcggcgc gaggccctcg gacaggtcga
     1381 tcaggaacct gacctggact tcctgcgccg tcccacggta ggccgcggag ggggtgatgc
     1441 cgtagatgta tttcccgacc agttgattgg tggcgtaatc gtagaggccg gcttcgttgg
     1501 cgtcatagcg gattccccag cggtagccgg gaaagggcgt cccgccctgg tactgctcct
     1561 cgaacagcac cgacttgacg tcttttaacc gcttgtcgta gccgaagagg agattgcccc
     1621 tggggccgtc gggcccgttg gccgggaacg agagcgaaac ccagcgctgg tagaggcgca
     1681 ctttgacgcc gccgtcatcg aaccccccct gtttcctttc gaccagaccg ggggcgaaga
     1741 cggcccgctc cgattccagc cggtcacagt aggccgagaa ctccatcatc aggttgtccc
     1801 aggaactgac gccgaccgtc tcgtagacga cccgttttac ctccggcgcg agcatggccg
     1861 gcatctcggt cgacgacccg gagagtttca ggtacaggtc gaagtagcgg ctgtagccca
     1921 tgcggtccag caggaaggcg gtgaacagcc cggccaccgg gtaggccatg tcggccccgg
     1981 cctgtttctc gaaacggtcc atcgacacga gcgagtccac ctggaccagc ccgttcttgt
     2041 gcaggaacgc cccgaggtag acaagcgacg tgggggcttt cccccaccgg ccgccgaggt
     2101 gcacggcgat cccctcgcgc acgatcggct gtgtcatgag cgggagcgag cggagcttga
     2161 ggttgaccag caggtgcacg acctcatggt tgtgcgggaa ggtagcgctg atgatgtcgt
     2221 tggaggccag gtcgtaggtg cctttggtca ggtgcccggt gatggtgagg acggtggagt
     2281 ccgacgtgca gtagaagaac tcgattttct tctcggcgat ctcgcgcagc acggccgccc
     2341 caaggctgag ggaatcggcg gcccgctcga tgaaccggtc ggcctcgtcc agcagcaccg
     2401 ggtgcaggta agcctcggcg ccgggctggg tgtggatacg gaagtacctg gtctccctta
     2461 ccggccagag cgccgccacc cagtcctgcg ggtaggtcag ccagaaatag gtgctgtccc
     2521 agtaggcata gtaggggtgt tccacctcct cgccgttgac agtcttcttg aatttcatcc
     2581 gcacgtaccg gccgccgtcg agcaggtcca cctgggtcac ggcggggacg aggtagtccc
     2641 gcataaccgg gagattgcgt accaccggcg agccggcgtc gattttcagg ggaatatcgg
     2701 tgaaggtgat atcgaaccgg ccggaccgct cgatggcggc ctgggtccag aatccctcgg
     2761 ccgaggccag gtttcccgag acgagcaggt cgaaatattc ctcgagcgtt tcaatcgcgg
     2821 gccgggtcga gaacggttcg gcggccggcg acaccgcggg ccggaaggcg acaagcaggg
     2881 cggcgagaac aaacgaacgg cctttcatcg attttctcct gttctggcgc ttggtggtac
     2941 ggtccccaat atacattctt tgtaccgtct atgtccagcc gggggtgcaa aaaaagcccc
     3001 ccgccggtcc ttgcgggggc ggg
//
LOCUS       DAOOKS010000183         3019 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_39618_length_3019_cov_2.676451, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000183 DAOOKS010000000
VERSION     DAOOKS010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39618_length_3019_cov_2.676451"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1097)
                     /locus_tag="PK112_03835"
     CDS             complement(<1..1097)
                     /locus_tag="PK112_03835"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPC11213.1"
                     /translation="MPLTKFRTALEAELAALEGQGLSKAHEHVIVRVHRPGGRVGTRV
                     FLEGHGDRPFLRMNANNYLGLSTHPRPIQAEEETVHSMGVGPGAVRFIAGTFQPHVEL
                     EEALARFHGREACMITSAAYTAVLGVLAALTTPETVIISDELNHNCIINAMKLARPKE
                     RKIYRHLDPADLERHLAASVGSADCALVVTDGVFSMRGDIAPLDVLQRIVERYDEKFP
                     RGAVLLVDDSHGVGTLGAAGRGAEELTRGRGDILIATLGKAFGVNGGYIASEASVVAY
                     LREKNPLYIFTNPITPPEAAAALQALAVVDSAEGRRLIAHLHFLTQRFEQGLVDLGFE
                     TISSPHPVVPLIVRDTERTRELVRYLYEYNIL"
     gene            complement(1305..2552)
                     /locus_tag="PK112_03840"
     CDS             complement(1305..2552)
                     /locus_tag="PK112_03840"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HPC11214.1"
                     /translation="MGFSDRVRATYQQQIRELQEAGLFKQERFIHSMQSPDIEVEFPS
                     GAPLKKVINMCANNYLGLSSHPSVVQAAHEGLDTRGYGMSSVRFICGTQDIHRELEDR
                     LSAFLGTEATILFPSCMDANAGVFEAILGKDDVMISDRLVHASIIDGIRLCSAVQDTF
                     KHSNMEHLEEKLQLHADKRCKVIITDGVFSMDGDTAKLDEMVALAEKYDAMVFVDDSH
                     STGFIGKTGRGTHEKCGVFGRIDIITTTFGKALGGASGGCVSGRRELVEMCRQRARPY
                     LFSNTIAPVVVAGVLKVLEILSASTERRDKLEANTAYWRKGLSEAGFVLKEGDTPIVP
                     VMLFNAKLSQDFSRDLFQEGIYAIGFFFPVVPQGQARIRTQISAAHERHHLDKALDAF
                     TKVGKKYNILGKTKQEIIEMFGQ"
     gene            complement(2625..2870)
                     /locus_tag="PK112_03845"
     CDS             complement(2625..2870)
                     /locus_tag="PK112_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HPC11215.1"
                     /translation="MDEMTKVVLDYVRREYLEDEDDELDVHTPLISGGIVDSFSMVSL
                     KRFLENKYKISIPDEKATPEAFDSVTKIVSLVREYVK"
BASE COUNT          501 a          994 c          973 g          551 t
ORIGIN      
        1 aggatgttgt actcgtacag gtagcggacc agctcccgcg tccgctcggt gtcgcgcacg
       61 atcagcggca cgaccgggtg ggggctcgaa atcgtctcaa accccaggtc gaccagcccc
      121 tgctcgaacc gctgcgtgag gaaatgcagg tgtgcgatga gccgccgccc ttcggcgctg
      181 tcgaccaccg ccagcgcctg cagtgccgcc gccgcctcgg gcggggtgat cggattggtg
      241 aatatgtaga ggggattttt ctcgcgcagg taggcgacca cgctcgcctc ggaggcgatg
      301 tacccgccat tgacgccgaa agccttgccc agcgtggcga tcagaatgtc gccgcgcccg
      361 cgggtcagct cctccgcgcc gcgcccggcg gccccgagcg tcccgacgcc atgggaatcg
      421 tcgaccagca gcacggcccc gcgcgggaac ttctcatcgt agcgctcgac aatccgctgg
      481 aggacgtcga gcggtgcgat gtcgccgcgc atcgagaata cgccgtcggt caccaccagc
      541 gcgcagtccg cgctcccgac cgaggcggcg aggtggcgct ccaggtccgc cggatcgagg
      601 tgccggtaaa tcttccgctc tttcgggcgg gccagcttca tggcgttgat gatgcagttg
      661 tggttgagct catcggagat gatgaccgtc tccggggtcg tcagcgccgc gagcacgccc
      721 aggaccgccg tgtaggcggc cgaggtgatc atgcaggcct cgcgcccgtg gaaccgcgcg
      781 agcgcctctt cgagctccac gtggggctgg aaggtgccgg cgatgaagcg caccgcaccc
      841 ggaccgaccc ccatcgagtg caccgtctcc tcctccgcct ggatggggcg gggatgggtt
      901 gacagcccga gataattgtt ggcgttcatg cgcaggaagg ggcggtcgcc gtggccctcc
      961 agaaacacgc gcgtgcccac cctcccgccg ggccgatgca cccgcacaat cacgtgttca
     1021 tgggctttgg agagcccctg cccctcaagc gcggccagtt cggcttccag cgcggtacga
     1081 aacttcgtca gcggcatctt tcgttcccgg ttatcccttc taaatcgcag acggccggcg
     1141 gttccggtcg tcggcgcggc aatgtatggt gtaacgggga cggcggcaag cggttcccgc
     1201 gccgccgccc gacaaaaaaa ctcaaggcag gaacccctta cgggttcctg ccttggacac
     1261 tcagacggcg gccggcagag cggcggccgc gggacgcccg gacctcactg gccgaacatc
     1321 tcgatgatct cctgcttggt cttgcccagg atgttgtact tcttccccac cttggtgaac
     1381 gcgtcgagcg ccttgtcgag gtggtgccgt tcgtgggcgg ccgaaatctg ggtgcggatg
     1441 cgggcctgcc cctgcggcac gaccgggaag aagaatccga tcgcgtagat gccttcctgg
     1501 aagaggtcgc gggagaaatc ctgcgacagc ttggcgttga acagcatcac cggcacgatc
     1561 ggcgtatctc cctctttgag cacgaacccg gcttcgctca gccccttgcg ccagtaagcg
     1621 gtgttggcct cgagcttgtc gcggcgttcg gtcgacgccg acaggatttc gagaaccttg
     1681 agcacaccgg ccaccacgac cggggcgatc gtattggaaa acaggtaggg ccgggcgcgc
     1741 tgccggcaca tctcgaccag ctcccgccgg ccggagacgc agccgccgga ggcgccgccg
     1801 agcgccttgc cgaaggtcgt ggtgatgatg tcgatccggc cgaagacgcc gcacttctcg
     1861 tgcgtgcccc gcccggtctt gccgataaaa ccggtcgagt gcgaatcatc cacaaacacc
     1921 atcgcgtcgt acttctccgc cagcgccacc atctcgtcca gcttggccgt gtcgccgtcc
     1981 atggaaaaca cgccgtcggt gatgatcacc ttgcaccgct tgtcggcgtg cagctgcagc
     2041 ttctcctcga ggtgctccat gttggagtgc ttgaaggtat cctgcacggc gctgcagagg
     2101 cggatgccgt cgatgatcga ggcgtgcacg aggcggtcgg aaatcatcac atcgtccttg
     2161 ccgagaatcg cctcgaacac cccggcgttg gcgtccatgc aggagggaaa caggatggtc
     2221 gcctcggtgc cgaggaaggc ggagaggcgg tcttcgagtt cgcggtgaat gtcctgggtg
     2281 ccgcaaatga aacggaccga ggacatgccg tagccgcgcg tgtcgagccc ttcgtgcgcg
     2341 gcctggacca ccgaggggtg gctcgagagg ccgaggtagt tgttggcgca catgttgatg
     2401 acttttttca acggcgcccc gctggggaat tccacctcga tatcgggcga ctgcatcgaa
     2461 tggatgaacc gttcctgttt gaacaggccg gcctcctgca gttcccggat ctgctgctgg
     2521 taggtcgcgc gcacgcgatc gctgaaaccc atgagtcttc tccttgccgg ccggtcgccc
     2581 ccgtcccgcc ccggtggcgg gtccggcggg cggccaccgg gcgcttactt cacatactcg
     2641 cgcacgagcg agacgatctt ggtcaccgag tcgaacgctt ccggagtcgc cttctcgtcg
     2701 ggaatcgaaa tcttgtactt gttctccagg aaccgcttga gcgagaccat cgagaaggag
     2761 tcgacgatgc cgccggagat cagcggcgtg tggacatcga gctcgtcgtc ctcgtcctcg
     2821 aggtactcgc gcctgacgta atccagaaca actttggtca tctcatccat ggtagtctcc
     2881 tgctccacac gagcgtgccg ctcggttaat cgttttccag cgtcgacaca tcgccgacct
     2941 cctcgcccca ctccctggcg tggatgaggc ggcgcatgat cttgccgctc cgcgtcttcg
     3001 gcagccccgg cacgtactc
//
LOCUS       DAOOKS010000184         3017 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_39655_length_3017_cov_3.397367, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000184 DAOOKS010000000
VERSION     DAOOKS010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39655_length_3017_cov_3.397367"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="PK112_03850"
     CDS             <1..203
                     /locus_tag="PK112_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013513699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11216.1"
                     /translation="LAIARQFDFAVVAEGVETTRSGHALANRHGVEMPITAQVHAVLF
                     DNKPPAEAVAELMGRELKPEIW"
     gene            229..579
                     /locus_tag="PK112_03855"
     CDS             229..579
                     /locus_tag="PK112_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HPC11217.1"
                     /translation="MKKTNANEKLYYSISEVARITGLEPYVLRYWEKEFPTLCPRKTR
                     GGNRIYTQKDIEVVNRIKHLRTEEKLTIAGARTKLTMRRPSESKETMVASARTKTLIG
                     QIRKDIEDILKDFS"
     gene            647..724
                     /locus_tag="PK112_03860"
     tRNA            647..724
                     /locus_tag="PK112_03860"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:682..684,aa:Pro,seq:ggg)
     gene            complement(818..2242)
                     /locus_tag="PK112_03865"
     CDS             complement(818..2242)
                     /locus_tag="PK112_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006337024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HPC11218.1"
                     /translation="MKKARPTEARGGRAAAPSAPLESLAGSLRGTLIRPGDSRYDAAR
                     SVWNSMIDRTPAAIVQAADSADVRHTVRFAREHRLPLSIRGAGHNIAGNAVCDDGLTL
                     DLSGLKAVQVEPAARRAWVGAGATLGDFDRAAQDFGLATPLGINSTTGIAGLTLGGGF
                     GWLTRKYGTAADNLRGAEVVLADGSLVRASDTEHPDLFWAIRGGGGNFGVVTRFEFDL
                     HPVGPNVYSGLVVYPHAEAKAVLRQYRDYVAELTDESACWLVLRPAPPVPFVPAEKHG
                     QNVLVVIFFHAGDPEAGRRAAEPLRHLGRPVGEALGVQPYTGWQSAFDPLMTAGARNY
                     WKTHNFTALPDALLDVLVRLADRVPSPECEVFLGQLGGAAARIPRETIAYPHRDADFV
                     LNVHARWRAAEDDERCIAWARELFRATEPFATGGAYVNFVTADESDRIKGIFGANYDR
                     LRRVKRAYDPDNLFRMNHNIAPGR"
     gene            2510..>3017
                     /locus_tag="PK112_03870"
     CDS             2510..>3017
                     /locus_tag="PK112_03870"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPC11219.1"
                     /translation="MDENRGEATLVLIPACNAGRYLDDLIPRCRRFVADEHLLVVNDG
                     STDDTLERLKRHRVNYISYPDNRGKGAALRAGYRYALARGYRSVVTLDADLQHLPEEL
                     PRFFAQDNGRRIVLGTRHMDPKVMPWDRRLTNNLTSLIISVFSAQRVRDSQSGYRLIP
                     TAVLRAIPL"
BASE COUNT          508 a          975 c         1059 g          475 t
ORIGIN      
        1 tcctcgccat cgcccgccag ttcgattttg ccgtggtggc cgagggagtg gagacgaccc
       61 gttcggggca tgccctggcg aaccggcacg gcgtggagat gccgatcacg gcgcaggtac
      121 acgccgtctt gttcgataac aagccgccgg ccgaggcggt ggcggagctc atgggcagag
      181 aactgaaacc ggaaatctgg taaggaaccg catcgcgagg agcggtccat gaagaagacc
      241 aacgccaacg agaagctgta ctactcgatc agcgaggtgg cccgcatcac ggggctcgaa
      301 ccttacgtgc tgcgatactg ggagaaggag tttccgacgc tctgtccgcg caagacgcgc
      361 ggggggaacc ggatctacac gcagaaggac atcgaggtgg tcaaccgcat caaacacctg
      421 cgcaccgagg agaaactgac gatcgccggg gcgcggacca agctgacgat gcggcggccg
      481 agcgagagca aagagaccat ggtggcctcg gcgcgaacga agacgctgat aggccagatt
      541 cgtaaggata tcgaggacat cctcaaagat ttctcttgaa ctcggcggcg gttttgatag
      601 attgggacgg gcgtaaccgg cgcgggacag tctagatccg atggatcgga gcgtagcgca
      661 gtttggttag cgcactcgct tggggtgcga gaggtcggcc gttcgaatcg gctcgctccg
      721 accattttgc cggccgggcg accggcggaa gctcccacga caacgaaaag gccggacgac
      781 gcgggcgcat cgcccggccc tgatgtcggc cgggcgctta ccgccccggc gcgatgttgt
      841 ggttcatccg gaacagattg tcggggtcat aggcgcgctt gactcgccgg agacggtcgt
      901 agttggcgcc gaagattccc ttaatgcggt cggactcgtc ggcggtcacg aaattcacat
      961 aggcgcctcc ggtggcgaaa ggctcggtcg cccggaacaa ctcgcgcgcc caggcgatgc
     1021 agcgctcgtc atcctcggcc gcccgccagc gggcgtggac gttgaggacg aagtcggcgt
     1081 cgcggtgcgg gtaggcgatg gtctcgcgcg ggatgcgggc cgccgccccg ccgagctggc
     1141 cgaggaagac ctcgcactcg ggactcggca cgcggtcggc caagcgcaca agcacgtcga
     1201 gcagggcgtc gggcagggca gtgaaattat gcgtcttcca gtaattccgg gcgcccgccg
     1261 tcatgagcgg gtcgaaggcc gactgccagc ccgtgtacgg ctggacgccg agcgcttcgc
     1321 ccaccggccg cccgagatgg cgcagcggct cggcggcccg ccggcccgcc tcgggatcgc
     1381 cggcgtggaa aaagatcacg acgagcacat tttgcccgtg cttttccgcc gggacgaacg
     1441 gaaccggcgg ggcggggcgc aagaccagcc agcacgcgga ttcgtcggtg agttccgcga
     1501 catagtcgcg atactgccgg agcaccgcct tcgcctcggc gtgcgggtag accaccaggc
     1561 cgctgtacac gttggggccg accgggtgca ggtcgaattc aaaccgggtg acgacgccga
     1621 aattgccccc gccgccccgg atcgcccaga acagatcggg atgctcggtg tcgctggccc
     1681 gcaccagcga gccgtcggcg agcacgacct cggcgccgcg gaggttgtct gcggctgtcc
     1741 cgtactttcg cgtgagccat ccgaagcccc cgccgagcgt gaggccggcg atgccggtcg
     1801 tggagttgat gccgagggga gtggccagtc cgaaatcctg ggccgcccgg tcgaagtcgc
     1861 cgagcgtggc gccggccccg acccaggcgc gacgggcggc cggctccacc tgcaccgctt
     1921 tcagcccgga caggtccagg gtgagaccgt cgtcgcagac cgcgttcccg gcgatgttgt
     1981 gcccggcgcc gcgaatggac agaggcaagc ggtgctcccg ggcgaagcgg accgtgtgcc
     2041 ggacatcggc ggaatcggcg gcctgcacga tggcggccgg tgtccggtcg atcatgctgt
     2101 tccagaccga gcgggccgcg tcgtagcggc tgtctccggg gcggatcagc gtgccccgca
     2161 gcgaaccggc gagcgactcg aggggggccg aaggggcggc ggctcggccg ccgcgcgcct
     2221 cggtgggacg ggcttttttc atcccgcgtc tccttcctgt gcggcagacc gcccggtcgc
     2281 ccgccgccga catgatttcc tgagcttggc cgcagccgat cggccgcggc cgcatgttgg
     2341 ccgttgtctg tccggtgata cgcggatccc cggccgtctg attgtggtcc aaatgagtct
     2401 ttaaaccaac gtccggccgg ctcgcagccc ggggctttcg cccgacctgc tctccgatcg
     2461 tgaacttttt gtgtcgcggg cgcccggccc ttccgtataa tgacctgcga tggacgagaa
     2521 tcggggcgag gcgacgctgg tgctcatccc ggcgtgcaac gccgggcggt atctcgatga
     2581 cttgatcccc cggtgccgcc ggttcgtggc cgacgaacac ctgctcgtgg tcaacgacgg
     2641 atcgaccgac gatacgctcg agaggctcaa acggcaccgg gtcaactaca tctcgtatcc
     2701 cgacaaccgg ggaaaagggg cggccctgcg ggcgggttac cggtatgcgc tcgcgcgcgg
     2761 gtaccgctcg gtcgtgacgc tcgatgccga cctgcagcac ctgccggagg agttgccccg
     2821 tttcttcgcc caggacaacg ggcggaggat cgtcctcggg acgcggcaca tggatcccaa
     2881 agtgatgccc tgggaccggc gcctcaccaa caatctgact tcgctgatca tctccgtgtt
     2941 ctccgcgcag cgcgtccgtg acagccagtc gggctaccgc ttgatcccga ccgcggtgct
     3001 gcgggcgatc ccgctgg
//
LOCUS       DAOOKS010000185         2988 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_40210_length_2988_cov_3.005796, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000185 DAOOKS010000000
VERSION     DAOOKS010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40210_length_2988_cov_3.005796"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="PK112_03875"
     CDS             <1..229
                     /locus_tag="PK112_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434229.1"
                     /note="negatively supercoils closed circular
                     double-stranded DNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HPC11220.1"
                     /translation="GRKGLSLQRYKGLGEMNPEQLWRTTMNPETRTLLQVTIDDAAEA
                     DHLFSVLMGTEIAPRSTFIQENARYVRNLDI"
     gene            complement(556..1035)
                     /locus_tag="PK112_03880"
     CDS             complement(556..1035)
                     /locus_tag="PK112_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11221.1"
                     /translation="MSFFGGSTEFPKGEAALAEDEAAVLDKLALKVVDWQMTAPAIIF
                     LESIKPLNFIGSQAMVFFEPIIQSVFAFKDYDTLRTALEKRSAIEVLLQRIEAHDAVA
                     KAREQRIKKYRREQKKTWKWYQRYLGVLQPRLTYPDDVLYGEEGKKPGDAGREAEKP"
     gene            complement(1032..1457)
                     /locus_tag="PK112_03885"
     CDS             complement(1032..1457)
                     /locus_tag="PK112_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11222.1"
                     /translation="MEWRVHPATRRPWLTAAVSLFILAVSFLVLVTTDSRWFSFFALV
                     VLFASLAKFYFPTSYRMDGRGVTIKTTTQTLLKEWRLFRSYYPDRNGVLLSPFPQPSR
                     LENFRGLYVLFGGNREDVVAFVSRHVGAAAPAGDKEATP"
     gene            complement(1531..1737)
                     /locus_tag="PK112_03890"
     CDS             complement(1531..1737)
                     /locus_tag="PK112_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11223.1"
                     /translation="MEERKYALKMVVNGKEREVSFEELALSNNLAQEALVRLLIEKKV
                     FQPEEMLRMLEKVKDERYRSPDQR"
     gene            complement(1791..2702)
                     /locus_tag="PK112_03895"
     CDS             complement(1791..2702)
                     /locus_tag="PK112_03895"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPC11224.1"
                     /translation="MIGPLTSEEAAVAAARLHGADLPLVIPAATDAGLTRLSESSFQL
                     SPNIELQAVRMADYAIDVLRAATAAIIAPAGGEHQRMAERFAERFRQRGGAVAASEYY
                     GPRDRDYGPCLRDLKGALLGEVADSSVWLDNRGDTIEADGIAAEVDVLYLPGRAEQIR
                     MLLPQLRFHNLRGRYLGSDGWGDEEIFRLGDDVTQHAVFPSPFLPAPPSEEAMRFAAA
                     YDERYGAEPERLARLGYDAMQLIVRAVERGRDSRRETARLLAAVRDYAGAAGAVTFGE
                     QRENVAIPLYRIADGAAVPLAEPAPAE"
     gene            complement(2699..>2988)
                     /locus_tag="PK112_03900"
     CDS             complement(2699..>2988)
                     /locus_tag="PK112_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11225.1"
                     /translation="GVAAELMASCGWTLDTAGIDRSDAGADAIEVAVVLPLSGELQEF
                     GNEIYRGALVGASMYAAQSGRQVVLAPYDTEGTRSPRPGWSASWRAARRTA"
BASE COUNT          450 a         1106 c          938 g          494 t
ORIGIN      
        1 cggccgcaaa ggcctgtcgc tgcagcgcta caagggtctc ggcgagatga accccgaaca
       61 gctctggcgg acgacgatga acccggagac gcgcacgctc ctgcaggtga cgattgacga
      121 cgccgccgag gccgatcacc tcttcagcgt gctcatggga acggagatcg cgccgcgcag
      181 cacgttcatt caggagaacg cgcggtacgt gcgcaatctc gacatctgat cccggccgcg
      241 cgcgagcacc ggttcgcggg ccggcctgcg ccggcccggc cgtgggcccg cccgctctcc
      301 gccccggaaa gaatcctccc ttccccggcg cggcgccgta tattaatcct gtaacccaac
      361 tcggaggtgt acggtgaaaa tcagcggttg gtgcgtgctc atcctcgtcc tcctcgccgt
      421 gattgtgtgg ctcgccacgc tcgacgagga ggacttcgcc cggtcctggg ccaaggtcaa
      481 gaaggactga cgggttgttc tgacgccggc ggcgccggga cgtggccgcc tgaactcgcg
      541 ccggatgacg gccccctacg gtttctccgc ttcccgtccc gcgtcccccg gtttcttccc
      601 ctcctccccg tagagcacgt catcggggta cgtaaggcgc ggctgcaaaa ctcccaggta
      661 gcgctggtac cacttccagg tcttcttctg ctcccggcgg tacttcttga tccgctgctc
      721 gcgcgctttg gccaccgcgt cgtgcgcctc gatccgctgg aggagcactt cgatcgccga
      781 ccgcttctcg agcgccgtgc ggagggtatc gtaatctttg aaggcgaaca cgctctggat
      841 gatcggttcg aaaaacacca tggcctgcga cccgatgaaa ttgagcggtt tgatcgactc
      901 caggaaaatg atcgccggcg ccgtcatctg ccagtccacc accttcagag cgagcttgtc
      961 cagcaccgcc gcttcgtcct cggccagcgc cgcttcccct ttcggaaact ccgttgatcc
     1021 gccgaagaaa ctcacggcgt cgcctccttg tctccggccg gcgccgccgc ccctacgtgc
     1081 cggctcacga aggccaccac gtcctcccgg ttccccccga acagcacgta cagcccgcgg
     1141 aaattctcca gccgcgacgg ctgcgggaag ggggagagga gcacgccgtt gcggtcggga
     1201 tagtagctcc ggaaaagccg ccactccttg agcagcgtct gcgtcgtcgt cttgatcgtc
     1261 accccgcgcc cgtccatgcg gtagctcgtg gggaaataga acttggccag cgaggcgaac
     1321 agcaccacga gggcgaagaa cgagaaccag cgcgaatcgg tggtcacgag gacaaggaag
     1381 ctcacggcga ggatgaacag gctcaccgcc gccgtcagcc acggccgcct cgtcgccggg
     1441 tgtacccgcc actccatcgc cgggccgtcc gccgggaccg cttgcgtccc gccggcgcgt
     1501 tcggccgggg tgtttgctcc gccgtccata ctaccgctga tcgggcgaac ggtaccgctc
     1561 gtccttcacc ttctccagca tccgcagcat ctcctccggc tgaaacactt tcttttcgat
     1621 gagcagccgg acgagcgcct cctgggcgag gttgttcgac agcgccagct cctcgaacga
     1681 cacctcgcgt tccttgccgt tgaccaccat cttgagcgcg tacttccgtt cttccacttc
     1741 cgactccagt ccgtccgtta caggtttcgt ttgcggcgca ggggcggggg tcactccgcg
     1801 ggcgccggtt cagcaagcgg caccgccgcg ccgtcggcga tgcggtaaag cgggatcgcc
     1861 acgttctcgc gctgttcgcc gaacgtcacc gcccccgccg cgcccgcgta gtcgcgtacc
     1921 gcggccagca ggcgcgccgt ctcgcggcgc gagtcgcgcc cccgctcgac cgcccgcaca
     1981 atcagctgca tcgcgtcgta gcccagccgg gccaggcgct ccggttccgc tccgtaccgc
     2041 tcgtcgtagg ccgccgcaaa ccgcatcgct tcctcggacg gcggcgccgg caagaacggc
     2101 gagggaaaca ccgcgtgctg tgttacgtcg tcgccgagcc ggaagatctc ttcatccccc
     2161 catccgtccg acccgagata cctcccccgc aggttgtgga accggagctg cgggagcagc
     2221 atgcggatct gttccgcccg ccccgggagg tagagcacgt ccacctccgc cgcaatcccg
     2281 tcggcttcga ttgtgtcgcc gcggttgtcc agccagaccg atgaatccgc gacctcgccg
     2341 aggagcgccc ccttgaggtc gcggaggcag ggcccgtagt ccctgtcgcg cgggccgtag
     2401 tactcgctcg ccgccaccgc gccgccccgc tgccggaagc gctcggcgaa ccgctccgcc
     2461 atccgctggt gttcgccgcc ggccggggcg atgatcgccg ccgtcgccgc ccgcagcacg
     2521 tcgatcgcgt agtcggccat ccgcacggcc tgaagctcga tgttggggga gagctggaac
     2581 gaggattccg agaggcgggt gagccctgcg tcggtggccg ccgggatgac cagcggcaga
     2641 tcggcgccgt gcagccgggc cgccgccacc gccgcctcct cgctcgtgag cggtccgatc
     2701 acgccgtccg ccgtgccgcc cgccagctcg ccgaccagcc gggccgcggc gatcgcgtcc
     2761 cctctgtatc atagggcgcc agcactactt gccggccgct ctgggcggcg tacatcgagg
     2821 ccccgacgag cgccccgcgg tagatctcgt tgccgaattc ctgcagctcg cccgagagcg
     2881 gcagcacgac cgccacttcg atggcgtcgg cgccggcgtc cgaccggtcg atcccggcgg
     2941 tgtcgagggt ccacccgcac gaggccatga gttcggccgc gacgcccg
//
LOCUS       DAOOKS010000186         2978 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_40388_length_2978_cov_3.310982, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000186 DAOOKS010000000
VERSION     DAOOKS010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40388_length_2978_cov_3.310982"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="PK112_03905"
     CDS             complement(<1..429)
                     /locus_tag="PK112_03905"
                     /inference="COORDINATES: protein motif:HMM:NF014880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HPC11226.1"
                     /translation="MALFNPLDKLKNSLARTKDKLLGRLSEVVRRRAIDDDLLEDIEE
                     ILVQADVGLPATERLIARVKEAARERRLTEGDQIIDLLKEEIVAILSRTGRPLFPPGE
                     PRPAVWLIVGVNGVGKTTTIGKLATLFSREGKKVSIAACDT"
     gene            complement(438..>2978)
                     /locus_tag="PK112_03910"
     CDS             complement(438..>2978)
                     /locus_tag="PK112_03910"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11227.1"
                     /translation="EEDTLREQEQELAALLEDFRGQMESHRGEAQTAADQLAAARQKL
                     ADVTARKASAEQALTDLTVRLESLLDLSGELAEELSSLAASLEAGQARKHLLEDMILQ
                     FDGYKSGVVAVLEEADRWEGVLGTVADRFVPQDGLEIAAEAALGDMAGYLICDRRETA
                     ERIIDHLRAESRGRIGILVPDTGMLNPVTRRPDLSAYPQFIGWLDQFISTADNLRPLM
                     EAVLARTAVFAAGQDPAPILERLPFGFQAVSTDGRVYAKNIIAGGSDDAFPLFRRKEK
                     VAEQQREIDELQARQADTHRRKNEVTAQIAAARAESGSLARGIEDIAEEMEAARAAIA
                     EIEFRQRTLQADLERMDRERRQAGDKLERIRHRQYSLGLDHSRLAGQKDSLVSTMTAT
                     DERLADLERAAVLAADRVSRLQVAAVEARSRIEQTESRRTHVAELLDEIARATAEKTE
                     EIGRAEQEISDSARRINELEAALRETFAERTRVTDQQAALRAEQAEIMDRLTGCEKQL
                     KELRAERESLSDRRHEKEMRLNTLESEARAIGDRVREDYGVDVAAIEAPKPDPAVDDQ
                     DAVHILAEQKERLKEFGAVNLLALEEYKTAAEREKFLGEQLTDLTTARNDLQTTINKI
                     NQTARKLFHDTFEQVRGNFRKLFVELFSGGEADIRMIDPDDPLESDIDITARPRGKKL
                     LSITMMSGGERALTAISLLFSLYMVKPSPFCILDEIDAPLDDANCRRFLRIIDTFSAQ
                     TQFIIITHNKITMAAADNLYGVTMGQPGISQLVAVRFSSDDAGTTTVDTERPIEIGAR
                     AGAGEPEPPRPRPSTKDREPEPEFELPQVIRDRLQQPAAPDAESDNAS"
BASE COUNT          404 a          936 c         1079 g          559 t
ORIGIN      
        1 cgtgtcgcag gcggcgatgc tgaccttctt cccctcgcgc gagaaaagcg tggccagttt
       61 tccgatagtg gtggtcttgc cgacgccgtt gacgcccacg atgagccaaa cggccggccg
      121 gggttcgccc gggggaaaca gcgggcggcc ggtgcgggag aggatcgcga cgatctcctc
      181 cttgagcagg tcgataatct ggtccccctc ggtgagccgc cgctcccgcg cggcctcttt
      241 cacgcgggca atgaggcgtt cggtggcggg caggccaaca tcggcctgga cgaggatctc
      301 ctcaatgtcc tcaaggagat cgtcgtcgat ggcgcgccgg cgaacaacct cgctgagccg
      361 gccgaggagc ttgtccttgg tgcgggccag cgaattcttg agcttgtcga gcggattgaa
      421 caaggccatg ggcgcggtca actcgcgtta tcggattcgg cgtccggagc cgccggttgt
      481 tggaggcggt cgcggatcac ctggggcagc tcgaattccg gttccggctc ccggtccttt
      541 gtggacgggc gcgggcgcgg cggttccggt tccccggcgc ctgcgcgcgc cccgatctcg
      601 atcggccgct cggtgtcgac cgtcgtagtg cccgcgtcgt cggaactgaa gcgaacggcg
      661 acgagctgcg agatgccggg ttgtcccatg gtcacgccgt agagattgtc cgccgccgcc
      721 atcgtgatct tgttgtgggt gatgatgatg aactgcgtct gcgcggagaa agtgtcgatg
      781 atgcggagga agcgccggca gttggcatcg tcgagcggcg cgtcgatctc atcgagaatg
      841 cagaacggcg agggtttgac catgtagagc gagaagagga gggagatggc ggtgagggcc
      901 cgctcgccgc ccgacatcat cgtgatcgaa aggagtttct tgccccgcgg gcgggccgtg
      961 atgtcgatgt cgctctccag cggatcgtcg ggatcgatca tgcggatgtc ggcctcgccg
     1021 cccgagaaga gctcgacaaa gagcttgcgg aagttgccgc gcacctgttc gaaggtgtcg
     1081 tggaagagct tgcgggccgt ctggttgatc ttgttgatcg tcgtctgcag gtcgttgcgg
     1141 gcggtcgtga ggtcggtgag ctgctcgccg aggaatttct cccgctcggc ggccgtcttg
     1201 tattcttcga gagccaggag gttgaccgcg ccgaactctt tgaggcgctc tttctgctcg
     1261 gcgaggatgt ggacggcatc ctgatcgtcg acggccgggt cgggcttggg cgcctcgatc
     1321 gcggcgacgt cgactccgta gtcctcgcgg acgcggtcgc cgatggcgcg ggcctccgac
     1381 tccagcgtgt tcaggcgcat ctccttttcg tggcggcggt cggagaggga ttcccgttcg
     1441 gcgcggagtt ccttgagctg tttctcgcag ccggtgagcc ggtccataat ctcggcctgc
     1501 tcggcgcgga gggccgcctg ctgatcggtg acgcgggtgc gttccgcgaa tgtctcgcgg
     1561 agcgcggcct cgagctcatt gatgcgccgg gcgctgtcgg aaatctcctg ttcggcgcgg
     1621 ccgatctcct cggtcttctc ggccgtggcg cgggcgatct catcgagcaa ctccgcgacg
     1681 tgggtccggc ggctttcggt ctgctcgatg cggctgcggg cctcgaccgc cgcgacctgc
     1741 aggcgggaga cccgatcggc ggccagcacg gcggcacgct cgaggtcggc cagacgctcg
     1801 tcggtagccg tcatcgtgct caccaggctg tccttctgac ccgccaggcg gctgtgatcg
     1861 aggccgaggc tgtactgccg gtggcggatg cgttcgagct tgtcgccggc ctggcggcgc
     1921 tcgcggtcca tgcgctcgag atcggcctgc agcgtgcgct gacggaactc gatttcggcg
     1981 atggcggcgc gggccgcttc catttcttcg gcgatatcct cgatcccccg ggcgagcgat
     2041 cccgactcgg cccgggcggc ggcgatctgc gccgtcacct cgttcttgcg gcggtgggtg
     2101 tcagcctgcc gggcctggag ctcgtcgatc tcccgctgct gctcggccac cttctccttg
     2161 cgccggaaca gcggaaaagc gtcatcggag ccgccggcga tgatgttctt ggcatacacc
     2221 cggccgtccg tggacaccgc ctggaagccg aaggggaggc gttcgagaat gggcgccggg
     2281 tcctggccgg cggcgaatac cgcggtgcgg gcgagaacgg cctccatgag cgggcgcagg
     2341 ttgtcggctg tcgagatgaa ctggtcgagc cagccgatga actgcgggta ggccgagagg
     2401 tcgggccggc gcgtgaccgg attgagcatg ccggtatcag gcacgaggat gccgatgcgg
     2461 ccccggctct cggcgcggag gtggtcgatg atccgctcgg ccgtctcgcg gcggtcgcag
     2521 atcaggtacc ccgccatgtc gccgagggcc gcctcggccg cgatctccag accgtcctgc
     2581 ggtacgaagc ggtcggcgac cgtgccgagg acgccctccc agcggtccgc ctcctcgagc
     2641 acggcgacca cgcccgactt gtacccgtcg aattgcagga tcatgtcctc aaggagatgc
     2701 ttgcgcgcct ggccggcctc gagcgaggcg gccagcgagg agagctcctc ggccagttcg
     2761 ccgctgaggt caaggaggga ttcgagccga accgtgagat cggtgagcgc ctgctcggcc
     2821 gaggccttgc gcgccgtcac gtcggcgagc ttctgccggg ccgcggcgag ttgatcggct
     2881 gccgtctgcg cctcgccgcg atggctctcc atttgtccgc ggaagtcctc cagaagcgcg
     2941 gccagttcct gctcctgttc gcggagggtg tcttcctc
//
LOCUS       DAOOKS010000187         2975 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_40453_length_2975_cov_3.666781, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000187 DAOOKS010000000
VERSION     DAOOKS010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40453_length_2975_cov_3.666781"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="PK112_03915"
     CDS             <1..693
                     /locus_tag="PK112_03915"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HPC11228.1"
                     /translation="SRDEAEELADHIEIKTRLNGTAVEVQTNYLGITSRGRSFWQKVF
                     GKGEDAPGEVAYKVSVPVRSDVTVQGLAATITLASIEGAVRVENESGATRAEYIFGPV
                     TVSQPQGDIDLLWIEGDIRVKATTGTVTVNQVRGALDIATHTGEVHVRTELDSPRDFF
                     VATTSGKITFAIPPAASGRFLVETASGGIQSQVPLVIESVTRQQLIGSFGRGGPRINL
                     TTSSGDVTVAQY"
     gene            726..2024
                     /locus_tag="PK112_03920"
     CDS             726..2024
                     /locus_tag="PK112_03920"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HPC11229.1"
                     /translation="MRTAVRKFGLSLLLAGLLAGPRQFPAVLAQEAPDERAAESSLAA
                     DLPADVAGPATAPPGIVFFEIRLSEDRAVATDTSGREWVYDFNADVWVEGTPGQEGGR
                     LTDRLQPRTTLPIDLRAICLVAVKPFERSVTIGYDEYVDGDIIATGRVTVKGWVKGDV
                     RSIDKRVLVTETGQVDGSVEAPSVIIRPGGVVSGTVRETGGPLDVESLTRAVSVDALV
                     LATALACGLLFFGFLVLAVAPRQAGRIDACITGHRVRTCALGFLFSLLLPVIIALFII
                     TLVGILVVPFVPLVYAGAIGMGFVSFGGTIGGWVLRRLTGARQGTQLRTALGTLLLAL
                     LWLLVIVLLGGGNAFLLGLGYALLAAAIPVTSFPVFAGIGGAILTRFGFRDYVSWKER
                     KPADTGPPAPAPPPLHEPPPIITPWPPAPEPPERREPPQE"
     gene            2107..2958
                     /locus_tag="PK112_03925"
     CDS             2107..2958
                     /locus_tag="PK112_03925"
                     /inference="COORDINATES: protein motif:HMM:NF028425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toast rack family protein"
                     /protein_id="HPC11230.1"
                     /translation="MRGVFRFWLLAALAALPAAALQARERKNTPVAAEGAERINVQID
                     FAAGKLRIDPGEIAEAAVFDFAYDEDKVDCAVDYEVRGGVGMLQAESERLSGSGGDTG
                     DNEWEVVFSRAYPMNLSLDIGACDAEIELGGLRLEEVAVEFGAASAEIGFREPNPARM
                     RELRFEAGASSVTATDLGNANFESCEFEGGVGSFDLDFRGEYHGESEIRVKVGLGSAE
                     ITLPVDIPCRIEGPGGGLLSSVEIHGGGLREIADDVWESDDYAGASTRLRLILELGLG
                     AVDVRFR"
BASE COUNT          461 a          931 c         1076 g          507 t
ORIGIN      
        1 agccgcgacg aggccgagga actggccgac cacatcgaaa tcaagacccg ccttaatggg
       61 acggcggtgg aagtacagac gaattacctg ggcatcacga gccgcgggcg ttcgttctgg
      121 cagaaagtgt tcgggaaggg agaggatgcg cccggagagg tggcctacaa agtctcggtg
      181 ccggtccgca gcgacgtgac cgtccaggga ctcgcggcca cgatcaccct cgcctcgatc
      241 gagggcgcag tcagggtgga aaacgagtcc ggcgcgacgc gggccgaata tatcttcggc
      301 ccggtgaccg tcagccagcc ccagggagat atcgacctcc tgtggatcga gggggacatt
      361 cgcgtgaagg cgacgaccgg gacggtgacg gtaaaccagg tgcggggggc gctcgatatc
      421 gccacccaca cgggagaggt gcatgtccgg acggagctgg acagcccgag ggacttcttt
      481 gtcgcgacca ccagcggcaa gattacgttt gcgattccgc cggcggcctc cggccgcttc
      541 ctcgtagaga cggcgagcgg aggaatacag agccaagtgc cgctcgtgat cgagtcagtg
      601 acgcggcagc agttgatcgg gagtttcggc cgcggcgggc cgaggatcaa cctgacgacg
      661 tcatcgggcg acgtcacggt ggcccagtac tgatcgttcg cggcgttgga acataggacg
      721 tggccatgcg caccgcagtg cgaaagttcg gtttgtccct tcttctcgcg ggcctcctcg
      781 cgggcccccg ccagttcccg gccgtcctgg cccaggaggc gccggatgag cgtgcggcgg
      841 aatcgtcgct cgccgccgat ttgcctgccg acgtggccgg tccggccacc gccccccccg
      901 gcatcgtttt tttcgaaatc cgtctctccg aagatcgcgc cgtggccacc gacacctcgg
      961 gtcgcgaatg ggtgtacgac ttcaacgccg atgtctgggt ggaagggacg ccggggcagg
     1021 aggggggaag gctcaccgac cggctgcagc cgcgcaccac cctgccgatc gatctccgcg
     1081 ccatctgcct ggtggcggtg aagccgttcg agcgctcggt gacgatcggg tatgatgagt
     1141 atgtcgacgg cgacatcatc gccaccggcc gggttacggt caaggggtgg gtgaaggggg
     1201 atgtccggtc gattgacaaa cgggtactgg tgaccgagac cggacaggtg gacggcagcg
     1261 tcgaggcgcc cagcgtgatc attcggccgg gcggagtggt gagcgggacg gtgcgggaga
     1321 cgggcggccc gctcgacgtg gagagcctga cccgggccgt ctcggtcgat gccctcgttt
     1381 tggcgaccgc cctggcctgc gggctgctct ttttcggctt tctcgttctg gcggtggctc
     1441 cgcggcaggc gggacggatc gacgcctgca tcaccggcca ccgcgtccgc acctgcgccc
     1501 tggggttcct gttttccctc ctgctccccg tcatcatcgc gctcttcatc atcaccctcg
     1561 tgggtatcct cgtcgtgccg ttcgtgccgc tggtgtatgc gggcgcgatc ggaatggggt
     1621 ttgtgtcgtt cggcgggact atcggcgggt gggtgctgag gcggctcacc ggggcccgcc
     1681 aggggacgca gttgcgcacc gcgctgggaa cgcttctgct ggcgctgctc tggctcctcg
     1741 tcattgtcct gctcggcggg ggtaacgcct tcctgctcgg gctcggctac gccctcctgg
     1801 cggccgccat tccggtcaca agcttcccgg tgttcgccgg gattgggggg gcgatcctga
     1861 ctcggttcgg tttccgcgac tatgtgagct ggaaggagcg caagccggcc gacaccgggc
     1921 cgccggcgcc ggcgccgccg ccgctgcacg aaccgccgcc gatcatcacc ccttggccgc
     1981 cggcgcccga gccccccgaa cgccgcgaac ctccgcagga gtgagcgggg caacattcgc
     2041 tgcccccgtc cgtaccatcc ctcagcaggt gtcgtgattg atcggtcttg gaccggaagg
     2101 gagattatgc gcggcgtttt cagattctgg ctcctagcgg ccctcgcggc gttgccggcg
     2161 gcggccctcc aggcgcggga gcggaaaaat accccggtgg cggcggaagg ggcggaacgg
     2221 atcaacgtgc aaattgactt cgcggccggc aagctccgga tcgatccggg ggagatcgcc
     2281 gaggcggccg tgttcgactt cgcctacgac gaggacaagg tcgactgcgc cgtcgactat
     2341 gaagtgcgcg gcggcgtcgg aatgcttcag gccgagagcg agcggctctc ggggagcggg
     2401 ggcgacacgg gcgacaatga atgggaggtc gtgttctcgc gcgcctatcc gatgaacctg
     2461 agcctcgaca tcggggcctg cgatgccgag atcgagctgg gcgggctgcg gctggaggaa
     2521 gtcgctgtcg aattcggcgc cgcctcggcc gaaatcgggt tccgagaacc caacccggcc
     2581 cgcatgagag aactgcggtt cgaggccggc gcgagctcgg tgacggcgac cgacctgggc
     2641 aacgccaatt tcgaatcctg tgagttcgag ggcggtgtcg gctccttcga tctcgacttc
     2701 cgcggagagt accacgggga gtcggagatc cgggtgaagg tggggctggg ctcggccgag
     2761 atcactctgc ccgtggacat cccctgccgc atcgaaggac cggggggcgg cctgctgtcg
     2821 tcggtcgaga ttcacggcgg cggcctgcgg gagattgccg acgacgtctg ggagtcggac
     2881 gactacgccg gagcttcgac ccgtctccga ctgattctcg agctcgggct cggcgcggtg
     2941 gatgtgcgct tccgctgacg cgggcgaacg gagga
//
LOCUS       DAOOKS010000188         2975 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_40454_length_2975_cov_3.410274, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000188 DAOOKS010000000
VERSION     DAOOKS010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40454_length_2975_cov_3.410274"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..767
                     /locus_tag="PK112_03930"
     CDS             <1..767
                     /locus_tag="PK112_03930"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HPC11231.1"
                     /translation="MVVLSADHLIRPAEKLLKILQAGTAIAAQEDSLITIGIVPTRPE
                     TGYGYIKLGDLYKLQASIPVHYVSGFTEKPRAAVALDYYYSNKYLWNSGMFIWSARAI
                     LEAMRKCQPDLGRLLDDYSRHIGTPGQTEACRGLYAEANGISIDFAVLEKADNVLTIK
                     ADIIWDDVGDWRALERYKQKDRDNCVIVGDAVTHESFETTIYNDGDGLVATLGVSDLI
                     IVKANHITLVAHRTRSQDIKQLLAKINESEEYRKYL"
     gene            736..1380
                     /locus_tag="PK112_03935"
     CDS             736..1380
                     /locus_tag="PK112_03935"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HPC11232.1"
                     /translation="MSRKSTGSICSLLALALLLGSGCRWQQRTFGAGAAEAAAPGDTL
                     RFDPLDLPQDREIVPAAHPPAAANRRSETVVVGPDAGAVDAADSAALRAADSTAALGG
                     QVFKVQLFTSKLFAEARQARRVAEEIFDRPVTMDYEVPYFKVRVGAFPDRYQAESYAQ
                     RARTAGYPDAWVVAAAAAVRRAAPLYDETSRLPALGDSTGADTLPAGGGSPDGD"
     gene            1370..2005
                     /locus_tag="PK112_03940"
     CDS             1370..2005
                     /locus_tag="PK112_03940"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HPC11233.1"
                     /translation="MATRPRVEEIDPQAPARELINEAVTVLDGGGLVVAPTETRYGLL
                     ARADRKIPADRLARAKRRASEKPISIFVGTIEMTTHYAELRPTALRLARIFLPGPLTL
                     VLPAVGSWHPVIAPVGKIGVRVSASPVIQAIMERVEYPVTATSANISGDEENETVEQI
                     VEIFGDEVDLYLDGGPLTGMPSTVIDCTIVPPAVLREGAIDPAEIDRVVRG"
     gene            2002..2508
                     /locus_tag="PK112_03945"
     CDS             2002..2508
                     /locus_tag="PK112_03945"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HPC11234.1"
                     /translation="MSMHEPFTVLFVCTGNTCRSPMAEAALRTLLQKQRPGAFEVISA
                     GASAATGYPATMYAIEAAKIWNCDLSGHKSRPLTADLIDRAHLIFGMSARHVQEVVRM
                     RPAAKDKTFLLKNFPDPGPTGEEVEDPIGQALDRYNETFLEIGEYLGKHLPEIVKRID
                     EKANAAKD"
     gene            2492..>2975
                     /locus_tag="PK112_03950"
     CDS             2492..>2975
                     /locus_tag="PK112_03950"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HPC11235.1"
                     /translation="MPRKTKIQVTVVGMLILAGLAPLIYFTAAQIEGAAAGDSAYVAA
                     ESADSLDRPIDNVAFGVGERLQFDVGYGFINAGSASMEVARLIEYEGRPCYQIQTRAE
                     SNSFFSTFFRVEDRVESITDAIGLYPWRFEKDLREGSYRANRQYTFNHREGAVYYGKD
                     T"
BASE COUNT          547 a         1001 c          953 g          474 t
ORIGIN      
        1 tcatggtcgt gctgtcggcc gatcacctca tccgccccgc cgagaaactg ctgaaaatcc
       61 tccaggccgg aaccgccatc gccgcccagg aggacagcct gatcacgatc ggcatcgtcc
      121 ccacccggcc ggagaccggt tacggctaca tcaaactcgg cgacctctac aagctccagg
      181 ccagcatccc cgtccactac gtctccggtt tcacggagaa gccgcgggcg gccgtggcgc
      241 tcgactacta ctacagcaac aagtacctct ggaatagcgg catgttcatt tggtcggcgc
      301 gggcgattct cgaggcgatg cgcaagtgcc agccggatct cggccggctc ctcgacgact
      361 actcccgcca tatcgggacg cccgggcaga ccgaggcgtg ccgcgggctc tacgccgagg
      421 ccaacggcat ctcgatcgat ttcgccgtgc tcgagaaagc cgacaacgtc ctgacaatca
      481 aggccgacat tatctgggac gatgtcggcg actggcgggc gctcgagcgg tacaaacaga
      541 aagatcgcga caactgtgtc atcgtcggcg acgcggtcac ccacgagtct ttcgaaacga
      601 cgatctacaa cgacggtgac ggtctcgtgg ccacgctcgg cgtgtccgac ctcatcatcg
      661 tgaaggcgaa ccacatcact ctggtcgcgc accggacgcg gtcgcaggac atcaagcagc
      721 tgctggcgaa gattaatgag tcggaagagt accggaagta tctgtagcct gctggcgctg
      781 gccttgctgc tcggctccgg ctgccgctgg cagcagcgga cgttcggggc gggggccgcg
      841 gaggcggcgg cgccgggcga caccctgcgt ttcgacccgc ttgaccttcc gcaggaccgg
      901 gagattgtgc cggccgcgca cccgccggcg gccgccaacc ggcgcagcga aacggtggtg
      961 gttgggccgg atgccggcgc cgtcgacgcc gccgacagcg ccgcccttcg cgccgccgac
     1021 agcaccgcgg cgctcggcgg ccaggtgttc aaggtgcagt tgttcacgag caagctgttc
     1081 gccgaggccc gccaggcgcg gcgggtggcc gaggaaattt tcgaccgccc cgtcaccatg
     1141 gactacgaag tgccgtattt caaggtgcgg gtcggcgcgt tcccggaccg gtaccaggcg
     1201 gagagctacg cccagcgcgc ccgcaccgcg ggttacccgg atgcctgggt ggtggcggcg
     1261 gccgcggcgg tgcgccgggc cgcgccgctc tacgatgaaa ccagccggct gcccgcgctt
     1321 ggcgactcga ccggcgccga tacgctgccg gccggcgggg ggtcgccgga tggcgactag
     1381 gccgcgggtg gaagagatcg acccgcaggc gccggcgcgg gaattgatca acgaggcggt
     1441 gacggtgctg gacgggggcg ggctggtggt ggcgccgacc gaaacgcgct acggtctgct
     1501 ggcgcgcgcc gaccgcaaga tccccgccga ccgactggcc cgcgccaagc gccgcgcctc
     1561 cgagaaaccg atttcgatct ttgtcggaac tatcgagatg accacccact atgccgaact
     1621 gcggccgacc gccctgcgcc tcgcacgcat ctttcttccg ggtccgctca ccctcgtgct
     1681 cccggccgtg ggatcctggc accccgtcat cgccccggtc ggcaagatcg gcgtgcgcgt
     1741 ctccgcctcg ccggtcatcc aggcgatcat ggaacgggtc gagtatccgg tcacggcgac
     1801 ctcggccaac atctccggcg acgaagagaa cgagacggtc gagcagattg tggaaatctt
     1861 tggcgatgaa gtcgatttat acctggacgg cggtccgctg accgggatgc cctcgaccgt
     1921 aatcgactgc accattgtgc cgccggcagt gctccgggaa ggggcgatcg acccggcgga
     1981 gatcgaccgc gtagtaaggg gatgagcatg cacgaaccat ttaccgttct gtttgtctgt
     2041 acgggcaaca cctgtcgctc accgatggcg gaagcggcgt tgcgcacatt gctgcagaag
     2101 cagcggccgg gcgcatttga ggtgatctcg gccggcgcgt cggcggcaac tggctacccc
     2161 gcaacgatgt acgcgattga agcggcgaaa atctggaact gcgacctctc cggacacaaa
     2221 tcgcgacccc tgaccgccga cctgatcgac cgcgcacacc tcatcttcgg catgtccgcc
     2281 cgccatgtcc aggaagtggt ccggatgcgc ccggcggcga aggacaaaac cttcctgctg
     2341 aaaaacttcc ccgaccccgg cccgaccggg gaggaagtcg aagatcccat cggtcaggcg
     2401 ctcgaccgct acaatgaaac cttcctcgaa atcggcgagt atttgggcaa acacctgccg
     2461 gaaatagtaa aaaggatcga cgagaaggcc aatgccgcga aagactaaaa tccaggtgac
     2521 tgtcgtggga atgctcatcc tcgcggggct cgccccgctg atctatttca ccgccgccca
     2581 gattgaaggg gccgcggccg gcgactccgc ctacgtcgcg gccgaatccg ccgattccct
     2641 cgaccggccg atcgacaacg tcgctttcgg cgtcggcgaa cgccttcagt ttgatgtcgg
     2701 ttacggtttc atcaacgccg gatccgcctc catggaagtg gcccgcctca ttgaatacga
     2761 gggccgcccc tgctaccaga tccagacgcg ggcggagtcg aacagcttct tctcgacctt
     2821 cttccgcgtc gaggaccggg tggaatcgat caccgacgcc atcggcctgt acccctggcg
     2881 cttcgagaag gatctccgcg aggggagcta ccgggcgaac cgccagtaca cgttcaacca
     2941 ccgcgagggc gccgtctact acggcaaaga caccg
//
LOCUS       DAOOKS010000189         2973 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_40490_length_2973_cov_4.186429, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000189 DAOOKS010000000
VERSION     DAOOKS010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40490_length_2973_cov_4.186429"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..916)
                     /gene="trmD"
                     /locus_tag="PK112_03955"
     CDS             complement(113..916)
                     /gene="trmD"
                     /locus_tag="PK112_03955"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HPC11236.1"
                     /translation="MKFELITLFPDYFRLALGQSLLGKARERRLFDIEVINLRDFATD
                     RHRTVDDTPFGGGGGMLLKVEPLDRCLASLGYRRRGQDGPPHERERIILTSAAGATFR
                     QDAAVRYSLCERVTIICGHYLGVDERILQLYEVDEVSIGDYVLTGGEPAAAVLVDAVA
                     RLIPKVLGNFESALEDSYMNQLLGTPSYTRPAVYEGLAVPEPLLSGNHAEIRDYRRRR
                     ALERALERRPDLLAAAELTDEERAWIARRRREQGAPPADSAGAADENEM"
     gene            complement(906..1448)
                     /gene="rimM"
                     /locus_tag="PK112_03960"
     CDS             complement(906..1448)
                     /gene="rimM"
                     /locus_tag="PK112_03960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HPC11237.1"
                     /translation="MEYVRVGWLGRTRGVGGDVWVTPDTDFPERFLGLKEIFVRNRDQ
                     WERLAIAHALLIGGRPVLRFAGISSPEEARRLTNRELAVARDRVCELPAGSHYIFELV
                     GCAVYDEAAVLIGELVDVESYPANDVYVIRTPEGGRKCLPAARPFVKDIDTAGKRIVI
                     DPAGLFDAAGAAPRDTGNEI"
     gene            complement(1461..1691)
                     /locus_tag="PK112_03965"
     CDS             complement(1461..1691)
                     /locus_tag="PK112_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HPC11238.1"
                     /translation="MKEFVEYIVKSVVDNPDDVEVSEVQGSRTTVYELRVAQADLGKV
                     IGKAGQTAKAIRTLLAAAAARRGKRAVLEILE"
     gene            complement(1785..2195)
                     /gene="rpsP"
                     /locus_tag="PK112_03970"
     CDS             complement(1785..2195)
                     /gene="rpsP"
                     /locus_tag="PK112_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014974045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HPC11239.1"
                     /translation="MAVHIRLLRMGKKKQPFYRIVAADSRRARGGRFLEVLGTYNPIK
                     KPAAVSLLEDRVTKWLDDGAVPSDTVRSLLTQTGMMEKYARAKAGQDVSAIQIRPTIT
                     ERRKRTRRMKKATVAAAADAPKAEAAPPAGEKTE"
     gene            complement(2220..>2973)
                     /locus_tag="PK112_03975"
     CDS             complement(2220..>2973)
                     /locus_tag="PK112_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HPC11240.1"
                     /translation="RLHVDEEMMVELQQIKEQVRPHEILLVADAMTGQDAVNVAREFH
                     DRLAITGVVLSKLDGDARGGAALSIREVTGTPIKLASVGEKLGDLEPFHPDRLASRIL
                     GMGDIVTLVERAQEHIDQAQAEKMQQKLLRAKFDFEDFLEQMEQIKKMGPLDSLLQMI
                     PGVGKALKGVSVEPKEMERMAAIIKSMTIEERRNPGMIDGSRKKRIAAGSGNSVQTIN
                     QLLKQFTAMQKMFHNMNKGNLKGIPKGMLPFG"
BASE COUNT          443 a         1043 c          927 g          560 t
ORIGIN      
        1 cccgcagcgt atcgcccgag ccgaacgcgg ggacatcctc ccgcttcatg cttttttcga
       61 gttgggcaat gcgtttcatc gtagtatccc tttcaggttc aatatgtaat cgctacatct
      121 cgttttcatc ggcggcgccg gcgctgtcgg ccggcggcgc gccctgttct ctccgccgcc
      181 gggcgatcca ggcgcgctcc tcatcggtca gttcggccgc cgccagcagg tcgggccggc
      241 gttcgagcgc ccgctcaagc gcgcggcggc ggcggtagtc ccggatctcg gcgtggttgc
      301 ccgacaacag cggctccggc acggccaggc cttcgtacac ggcgggccgg gtgtagctcg
      361 gggtgccgag caactggttc atgtaggagt cttcgagcgc cgactcgaag ttccccagca
      421 ctttcgggat cagccgggcg accgcatcga ccagcaccgc cgcggccggt tcgccgccgg
      481 tcagcacata gtcgccgatc gacacctcgt ccacttcgta caactgcagg atccgctcgt
      541 ccacgccgag gtagtgcccg cagatgatcg tgacccgctc gcacagcgag tagcgcacgg
      601 ccgcgtcctg gcgaaaggtc gcacccgccg ccgaggtcag gatgatccgc tcccgctcat
      661 ggggcggacc gtcctgcccg cgccggcggt aaccgagcga ggccaggcac cggtcgagcg
      721 gctcgacttt caggagcatg ccgccgcccc cgccgaaggg ggtgtcgtcg acagtgcggt
      781 gccggtcggt ggcgaagtcg cgcaggttga tcacctcgat gtcaaagagc cgccgctcgc
      841 gcgcttttcc cagaagcgac tgcccgaggg ccaggcggaa gtagtccggg aagagcgtga
      901 tcagttcaaa tttcattgcc ggtgtcgcgc ggcgccgcgc cggccgcgtc gaacaggccg
      961 gcggggtcga tcacgatccg cttgccggcc gtgtcaatat cctttacgaa cggccgcgcc
     1021 gccggcaggc atttgcggcc gccctccgga gtccggatca cgtacacgtc gttggccggg
     1081 tacgactcca cgtccaccag ctcgccgatg agcaccgcgg cctcgtcgta caccgcgcag
     1141 ccgaccaact cgaagatgta gtggctgccc gccggcagct cgcacacccg gtcgcgggcg
     1201 accgccagtt cccggttggt cagccggcgc gcctcctcgg gcgaggagat ccccgcgaag
     1261 cggaggacgg gccggccgcc gatgaggagg gcgtgggcga tggcgaggcg ttcccactgg
     1321 tcgcggttcc ggacgaaaat ctctttcagg ccgagaaacc gctcgggaaa atcggtgtcg
     1381 ggggtcaccc agacatcgcc gccgacgccg cgggtgcggc ccagccagcc aacgcgcacg
     1441 tactccactg cggactccgg ctactcgagg atttccagca ccgcccgttt gccccgccgc
     1501 gcggcggcgg ccgccaggag cgtgcgaatc gccttggccg tctgcccggc cttgccgatc
     1561 actttcccca gatccgcctg ggccacccga agttcgtaga ccgtggtgcg gcttccctgg
     1621 acctcgctga cttccacgtc atccggattg tccaccaccg acttgacgat gtactcgacg
     1681 aattccttca tggctgggtc cttccttttt ccgtctccga aatcgcagtt cgcgtaagcg
     1741 gttcaggcta agttccgttg gctggtccgc gccggacggt cgcgctattc ggttttctct
     1801 cccgcgggcg gagcggcctc ggctttgggc gcgtcggccg ccgccgcgac ggtcgccttc
     1861 ttcatccgcc gagtgcgctt gcggcgctcg gtgatggtcg ggcggatctg gatcgcgctg
     1921 acgtcctggc cggctttcgc cctggcgtac ttctccatca tccccgtctg ggtcaacaac
     1981 gagcgcaccg tatccgaggg aacggcgccg tcgtccagcc acttggtgac ccggtcctcg
     2041 agcagactca ccgcggccgg cttcttgatc gggttgtagg tgccgagcac ttccagaaag
     2101 cgcccgccgc gcgcccggcg ggaatccgcc gcgacgatcc gatagaaggg ctgtttcttc
     2161 ttgcccatcc gaagcagtct aatgtgaacg gccaaaaatc cttcctcctt ctcacggcct
     2221 tatccgaaag gaagcatccc cttcgggatc cctttcagat ttcctttatt catattgtga
     2281 aacatcttct gcatcgccgt aaactgcttg agcagctggt tgatcgtctg caccgaattc
     2341 cccgagccgg cggcgatccg cttcttgcgc gacccgtcga tcatccccgg gttgcgccgc
     2401 tcctcgatcg tcatcgactt gatgatcgcc gccatccgct ccatctcctt cggctcaacc
     2461 gacaccccct tgagcgcttt tcccaccccg gggatcatct gcaggagcga gtcgagcggc
     2521 cccatcttct tgatctgctc catctgctcg aggaaatcct cgaaatcgaa tttggcccgg
     2581 agcagcttct gctgcatctt ctcggcctgc gcctggtcga tgtgctcctg cgcccgttcg
     2641 acgagggtga cgatgtcgcc catgccgagg atgcgggagg cgagccggtc gggatggaag
     2701 ggctcgaggt cgccgagttt ttcgccgacc gaggcgagct tgatgggcgt gccggtcacc
     2761 tcgcggatcg acagggccgc cccgccccgc gcatcgccgt cgagcttcga cagcaccacc
     2821 ccggtgatcg ccagccggtc gtggaactcg cgggcgacat tgaccgcgtc ctgccccgtc
     2881 atcgcgtcgg ccacaaggag aatctcgtgc gggcgcacct gctccttgat ctgctgcagc
     2941 tcgaccatca tctcctcgtc gacgtgcaga cgg
//
LOCUS       DAOOKS010000190         2973 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_40496_length_2973_cov_3.300891, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000190 DAOOKS010000000
VERSION     DAOOKS010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40496_length_2973_cov_3.300891"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="PK112_03980"
     CDS             <1..632
                     /locus_tag="PK112_03980"
                     /inference="COORDINATES: protein motif:HMM:NF014010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HPC11241.1"
                     /translation="AFATTLTMLEMDLRMFEASSAALRRSDSAELPFDVRQEDRRINK
                     YEREVRRNVLTHLSVAGTQNIVPGLVLVSIVIDVERIGDYTKNIAELAQLHPGRLEGG
                     PYEGEVAEIESQVNRIFPLVLRVLREQEAELARNIMAREDRTGKMAEAIVQSLISEKG
                     RGLPVAEAVSLALYARHLKRINAHLTNIASAVGNPFPRLGFRAKPDQGD"
     gene            complement(691..1929)
                     /locus_tag="PK112_03985"
     CDS             complement(691..1929)
                     /locus_tag="PK112_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015102139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HPC11242.1"
                     /translation="MADRPHRYDIDPYLWLILIGTFLSCLPGLYLVAAGKHLYAPYAA
                     VVFGVAIFGAAFILSWAAEVAQMDMSQSLAVAILALVAVLPEYAVDFVFTWKAAHDPT
                     QAHYALANMTGANRLLVGLGWPAVLFLYILAKRRREVRLDETQRVEIFYLAMATLYSF
                     TIPLKGSLNLIDTVVLVTLFGLYTRRAAQMPQEEPEMVGPVKIVANAATVPRRLFTAL
                     FFLFSGFVIFLVAEPFAHALVESGGHLGIDEFFLVQWLAPIASESPEFIVAATWALRG
                     QAGAALKALISSKVNQWTLLVGTIPLVYAISAGEIRPFVLDVPYGEGPTDVQTHELIL
                     TAAQSLFAVAVLVNLVLTRWDGVLLFGLFALQLVWQDIRMEVAAVYIVLAVIWHWVNR
                     RHLLPAARIGLGIRGRPGTS"
     gene            complement(1957..2862)
                     /locus_tag="PK112_03990"
     CDS             complement(1957..2862)
                     /locus_tag="PK112_03990"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPC11243.1"
                     /translation="MKLANLLMDRRIRIDLKAHTKAEAVDELLDLLRAERVALDYDDI
                     VRSVRIREEIESTSYGRGFAFPHARTNAVSELYILIGVSREGLDEGGPDGVPVHVVCL
                     LLTPSAIAKLYLQTLSGLATFARMPGSLDRVRSIINPRDLVKLVADTGVTVDKELMVK
                     DIMRHDVAKVRPDDTLKDVANEMFRHRLSALAVVDENNRLLGVISDRDLIKAALPDYR
                     TLISNLNYSLDVEPFEELLKREDKIKVSQLYREDFETTTPETRIVEVAAMMIFKDFTR
                     VFVTENDNLVGILLRKDIVNMIIRG"
BASE COUNT          539 a          986 c          975 g          473 t
ORIGIN      
        1 cggctttcgc gaccactctg accatgctag agatggacct ccgcatgttc gaggcctcga
       61 gcgccgcgct ccgccggtcg gactcggccg aactcccctt tgacgtccgg caggaggacc
      121 gccgcatcaa caagtacgag cgggaggtgc gccgcaacgt cctcacccac ctgtcggttg
      181 ccggcaccca gaacatcgtc cccggcctcg tgctcgtgtc gatcgtcatc gacgtcgagc
      241 ggatcggcga ctacacgaag aacatcgcgg agctcgccca gttgcacccg ggccggctcg
      301 agggcggccc gtacgagggg gaggtggcgg agatcgaaag ccaggtgaac cggattttcc
      361 cgctcgtcct gcgcgtcttg cgggaacagg aggccgagct cgcccgcaac atcatggcgc
      421 gggaagaccg cacgggcaag atggccgaag cgatcgtcca gtcgctgatt tccgagaagg
      481 gccggggcct gccggtcgcc gaggccgtgt cgctggcact ctacgcccgg cacctcaagc
      541 gcatcaacgc ccacctcacc aacatcgcca gcgcggtcgg caatcccttc ccccgcctgg
      601 gcttccgcgc caaaccggac cagggcgact gaacttgaga ggcgaacctg ttgggtgagg
      661 ggcgacgagg cggaggctgg atccggccgt ctatgaggtt cccgggcgtc cccggatccc
      721 cagcccgatc cgcgccgccg gcaggaggtg gcgccggttg acccagtgcc agatcacggc
      781 gagcacgatg tacacggccg ccacttccat gcggatatcc tgccacacca gttggagcgc
      841 aaacagcccg aacagcagca cgccgtccca gcgggtgagg acgagattga ccagcacggc
      901 cacggcgaag agcgactggg ccgccgtcag gatcagctcg tgcgtctgca cgtcggtcgg
      961 cccctcgccg tagggaacat cgagcacaaa ggggcggatc tcgccggccg agatggcgta
     1021 caccagtggg atcgtgccga ccaggagagt ccactggttg actttcgacg agatgagggc
     1081 tttgagcgcc gctccggcct gcccgcggag cgcccaggtg gcggcgacga tgaactcggg
     1141 cgattccgag gcgatcgggg ccagccactg cacgaggaag aactcatcga tcccgaggtg
     1201 tcctccgctc tccacgagcg cgtgggcgaa cggctcggcg acgaggaaaa tgacgaaccc
     1261 ggagaacagg aaaaacaggg ccgtgaacag gcggcgggga acggtcgcgg cgttggccac
     1321 gatcttcacc ggccccacca tctccggctc ctcctgcggc atctgcgccg cccgccgcgt
     1381 gtacagcccg aacagggtca cgagcacaac ggtgtcgatc aggttgaggc tccccttgag
     1441 cgggatcgtg aacgagtaca gggtcgccat ggcgagatag aagatctcca cgcgctgggt
     1501 ctcatcgagc cggacctcgc ggcggcgctt ggcgaggatg tacaggaaga gcacagccgg
     1561 ccagcccagc ccgacaagga gccggttcgc tccggtcatg ttggcgagcg cgtagtgcgc
     1621 ctgggtcgga tcgtgcgccg ctttccacgt aaagacgaag tccacggcgt actcggggag
     1681 caccgccacg agtgcgagga tggccaccgc cagcgactgc gacatgtcca tctgcgccac
     1741 ctcggccgcc cacgagagga tgaaggcggc cccgaaaatg gccaccccga acacgaccgc
     1801 cgcgtagggt gcgtagaggt gtttcccggc cgcgacgagg tacagccccg gcaggcagga
     1861 caggaacgtg ccaatcagaa tcagccacag gtaggggtcg atgtcgtagc ggtgcggtct
     1921 gtctgccatc cggtcctctc cgcgggcttt ggccgcctag ccccgaataa tcatgttcac
     1981 gatgtccttg cgcaggagga tcccgacgag gttgtcgttc tcggtgacga acacgcgcgt
     2041 gaaatccttg aaaatcatca tcgccgccac ctccacgatg cgcgtctccg gcgtcgtggt
     2101 ctcgaaatcc tcccggtaga gctgcgacac cttgatcttg tcctcgcgct tgagcagttc
     2161 ctcgaacggc tccacgtcga gcgagtagtt gagattcgag atgagcgtcc ggtaatcggg
     2221 cagggcggcc ttgatgaggt cgcggtcgct gatcacgccg agcaggcggt tgttctcgtc
     2281 gaccaccgcc agcgccgaca gacggtgccg gaacatttcg ttggcgacat ccttgagggt
     2341 gtcgtccggc cgcaccttgg ccacgtcgtg ccgcatgatg tccttgacca tcaactcctt
     2401 gtcgaccgtc accccggtgt cggccaccag tttcaccagg tcgcgcgggt tgatgatcga
     2461 ccgcacgcgg tcgagcgagc ccggcatccg ggcgaacgtc gccagccccg acagcgtctg
     2521 gaggtagagc ttggcgatcg ccgagggggt gaggagaagg cagacgacgt gcaccggcac
     2581 gccgtcggga cccccctcgt cgagcccctc ccgcgacacg ccgatgagga tgtacagttc
     2641 gctgaccgcg ttggtccggg cgtgggggaa ggcgaatccc cggccgtagg aggtgctctc
     2701 gatctcctcg cggatgcgca ccgatcgcac gatgtcgtcg taatccagcg ccacccgctc
     2761 ggcccggagc aggtcgagca gttcgtccac ggcctcggcc ttggtgtggg ccttcagatc
     2821 gattcggatc cggcgatcca tgagcaaatt cgcaagcttc acggtaaccc cttactgccg
     2881 gtgtcggcat ccttggcgga ccgttctttg aggagcgccg gcgtcaccac gccccccgag
     2941 acaatgaact tgaccccctc ttcgatcgtc atg
//
LOCUS       DAOOKS010000191         2968 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_40580_length_2968_cov_3.268795, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000191 DAOOKS010000000
VERSION     DAOOKS010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40580_length_2968_cov_3.268795"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="PK112_03995"
     CDS             complement(<1..338)
                     /locus_tag="PK112_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11244.1"
                     /translation="MSTSDLQRGRYRILLYRFLADSIPAVAACVGTWVRLAAAPGRFL
                     VDGDVSPSVRRQVEERLERLSAHVYRSVGGTRAGGADLPVDIAAGLFRDGFFGGFVTV
                     DATGTGVDRF"
     gene            761..1108
                     /locus_tag="PK112_04000"
     CDS             761..1108
                     /locus_tag="PK112_04000"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HPC11245.1"
                     /translation="MKAIIVLLAVTLVCAAQAVALPLSAPRLVDVSSIDWGAQITVAD
                     GSVGTTGQAYFTGDPRTSGSQATGTDGTGGVIIRPGNTTLPKTVTTVPEPATLVLVGL
                     GIAGLALLRRRKA"
     gene            1384..2142
                     /locus_tag="PK112_04005"
     CDS             1384..2142
                     /locus_tag="PK112_04005"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HPC11246.1"
                     /translation="MRILVLVLCIGFCLATPASAFNFHYYDFGDSPFDENNNWSPINY
                     PGGIGNLPSPGTLGEGGEEFDLEGLKVAVDQDYVYIALTNSFGYSAYSTGWGRNYNLG
                     DLFIGTNGGGYQYAIDLVNGGSNGLYRVTGRTGIPNIPGSYYGTPIAPLAGDYRMTSG
                     TYLGQVNTMLSFWDDYELNHLMPGDGDTYVWEFRFARSLLGDFQSLDFHVTLGCGNDL
                     MEKSVAMVPEPATVLLLGLGLSGVMLRRLRRRDA"
     gene            2313..>2968
                     /locus_tag="PK112_04010"
     CDS             2313..>2968
                     /locus_tag="PK112_04010"
                     /inference="COORDINATES: protein motif:HMM:NF024574.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPC11247.1"
                     /translation="MGRISRILILAAGFAAAFGCADNPALRLRFQAEKKLFDAEKIIA
                     QAQVNPREAPPELVRRAGEKLSAAAEFALAALDSVSEQANPVEYRELQQIAFQAGNEL
                     NRLLYNQRRYEESIAVLTRLLSQSALAREELMVTQVNLGRTLQASGRWDSALAMYTSA
                     LETFFPPVDSRGEIVKPLFDLPAHIFAVARQVGDSVEAAYHLERAVSYYTDLAAKTSD
                     R"
BASE COUNT          563 a          900 c          915 g          590 t
ORIGIN      
        1 atgaaccggt ccacaccggt cccggttgcg tccacggtca cgaagccgcc gaagaaaccg
       61 tcgcgaaaca acccggctgc aatgtcgacc gggagatcgg cgccgcccgc ccgcgtcccc
      121 ccgaccgaac ggtacacgtg cgcgctcagg cgctccagcc gctcttccac ttggcggcgg
      181 acagaaggcg agacatcgcc gtcgaccaga aaccggcccg gcgcggcggc caaacgaacc
      241 caggtcccga cacaggcggc caccgccggg atcgaatcgg cgagaaagcg gtacagcagt
      301 atgcgatagc ggccccgctg gaggtctgat gtgctcaagc gcagttggcg gtcgatttga
      361 ccgccggtgc tcagagcgga tgggggcggc acaagcaccc cggcggccaa ctgacccgag
      421 ttcctccgac tggcacacat agcaggctcc tccgaatttt ggcgtgagct ctttgccaga
      481 cgcgcaggcc ggatttgagg cgcggtcctg gtatgattgt gagccgagcg ggattacagg
      541 tgagagaaga cgctgcaagt ggtttgcaga aacagcgcag atgcgtgcac aagtgtgtgt
      601 gcagacagga agacgtcatg ttctcgggac gataaggtca aacagttaat attatgattg
      661 acaagatcat agcagaatgc tgtcgaattg ggtcaggtgg cacgaaaatt gctggaaaat
      721 tgcacaggag atctttaccc taaacaaaaa tgggtttcgg atgaaagcga tcatcgtcct
      781 tttggcggtc accttggtgt gtgcagctca agcggttgct ttgccgctga gcgctccacg
      841 gctggtcgat gtgtcttcga tcgattgggg cgcccagatc accgtcgccg acggttcagt
      901 cggcacgacc gggcaggcgt attttaccgg cgaccctcgg acgtcgggaa gccaggcgac
      961 cggtaccgac gggaccggcg gggtgatcat tcgaccgggc aacaccacgc tccccaagac
     1021 ggtcacgacc gttcctgagc cggccactct tgtgcttgtc ggtctgggta tcgcgggctt
     1081 ggcgttgctt cgccgccgca aagcttaacc gagcttcatt caatcgaatc gagaattctg
     1141 gggccacccg gatcagggtg gccttttcta tttcccgcag gtcgtggtga cacggattgt
     1201 cgttgttgtg caacttgttg cagggaaagc caggccgggc cacggagtac agtctccgct
     1261 ctgtcgtata agtaacggtg tcacaatatc ttatgatgca cactgagcag ggggaaagcc
     1321 aacggcattg cagttgctaa aactttgaac agatagaagt agaacaactg cgaaagggca
     1381 accatgcgga tccttgttct tgtgttgtgc attggctttt gtctggcgac tcccgcctcg
     1441 gccttcaatt ttcactacta tgattttggc gactcgccgt tcgacgagaa caacaactgg
     1501 tcgccgatca attaccccgg cggaataggc aacctgccgt cgcccggtac cctcggcgag
     1561 gggggcgagg agttcgacct cgagggtttg aaagtagcgg tggatcagga ctatgtgtac
     1621 atcgccctga ccaactcgtt cggttactcc gcctattcga ccggctgggg ccgcaactac
     1681 aacctgggcg acctgttcat cggcaccaat ggcggcggtt accagtatgc cattgatctg
     1741 gtcaacggcg gctcgaacgg cctgtatcga gtaaccggcc gcaccggtat cccgaatatc
     1801 cccggttcgt actacggcac gcccatcgcc cccttggccg gcgactaccg gatgaccagc
     1861 ggcacttacc tcggccaggt gaatacgatg ctcagtttct gggatgacta cgagctcaat
     1921 catctgatgc cgggtgacgg cgacacgtac gtgtgggagt tcaggtttgc ccgttcgctg
     1981 ctcggggatt tccagtcgct cgattttcac gtcactctgg gttgcggcaa tgacctgatg
     2041 gagaagtcgg tggcgatggt tccggagccg gcgaccgttc tccttctggg actggggctg
     2101 tcgggggtga tgctccgccg cctgcgtcgc cgcgacgcat gatcccttga cccataagat
     2161 ttgcaggcgg gccgcccgga gttattcggg cggcccgtgc gctttggtgg ccgcaggcgc
     2221 cgctgttttt tgacttgttc ccgtttcccc cgcccgtata tttgcctcgg actctgtggc
     2281 aacggtttgt cagactgccg ataataacgc aaatgggaag aatctcgcga attctcattc
     2341 tcgccgccgg gttcgccgcc gctttcggct gcgccgacaa ccccgccctg cgcctgcgct
     2401 tccaggcgga gaagaagctc ttcgatgccg agaagatcat cgcgcaggcc caggtgaatc
     2461 cccgcgaggc tccgcccgag ctggttcggc gggccgggga gaagctctcc gcggctgcgg
     2521 agttcgccct ggccgcgctc gactcggtca gcgagcaggc caacccggtc gagtaccggg
     2581 agctgcagca gatcgcgttt caggccggca acgaactcaa ccgcctgctc tacaaccagc
     2641 ggcggtacga ggagtcgatc gccgttctca cccgtctcct ctctcagtcc gccctggcgc
     2701 gggaagaact catggtgacc caggtcaacc tcgggcgcac cctgcaggcc tccggccggt
     2761 gggacagtgc gctggcgatg tacacctcgg cgctggaaac gttcttcccg ccggtcgaca
     2821 gccgcggcga aatcgtcaag ccgctgttcg acctgcccgc ccacatcttc gccgtcgccc
     2881 gccaagtggg ggattcggtg gaagctgcct atcatctgga gcgggcggtg agctactaca
     2941 ccgacctcgc cgcgaagact tccgaccg
//
LOCUS       DAOOKS010000192         2964 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_40653_length_2964_cov_3.443451, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000192 DAOOKS010000000
VERSION     DAOOKS010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40653_length_2964_cov_3.443451"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1693)
                     /locus_tag="PK112_04015"
     CDS             complement(<1..1693)
                     /locus_tag="PK112_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11248.1"
                     /translation="MRRLLVVVGCLLLPVLASSPRAERGQARLVPVPDGAARAELRPV
                     PSLATADTCIVRHDAGIYYKIDGWVTGNELYKGYCDPALTCPNPYPFIVTEINMPMYF
                     TGTPTFGVSVDVELADNSDPSCPMPGPLVAISSEYTLQVPAEGLYDIWIPLDTPVTVT
                     GPFFAGFFLSTPFAEADGAAVVIDTIPELCVSYNIWDTAVGFIDLGDNEIWNFPGRLV
                     LYAAGVPIGTANPPDVRLLSPRPSDTLFGPTEIWIQEKGGSPILDYAVFEYRPAGGGA
                     YVEIGRDFDPTAAFRDGASATAASAAGLSYAFNFGALTEGMWTLRATVYDTLGRFDRD
                     SVTVYLEPTPPVPTVISHEDGGSFCTPLTFNLVCSDEDVDYTQGFRRGALDEYSAGVI
                     VAGPPARGPHWNAPYAAAAAIELWYDRGTTRPMTENNLKLSLDTLAARLAYWMETTVN
                     TGTWDEGFLAGLNEYLHVKGDDLYTAYRRRPNYFQIRDWAENWERSVLLGLAGTPGQW
                     VVVDGFSGWLQVDGTYLVRIMDPLTGTAAPVSLRHNMGTAEVFVGGAWRPVDLMVS"
     gene            1788..2666
                     /gene="lipA"
                     /locus_tag="PK112_04020"
     CDS             1788..2666
                     /gene="lipA"
                     /locus_tag="PK112_04020"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012824133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HPC11249.1"
                     /translation="MADSPPSRPPGGGPRKPAWLRVTAFSGMGYENVSRLLRHYGLNT
                     VCHAANCPNRGECFNRGTATFLIMGPTCTRNCRFCDIEPGRPRPLDPTEPARVAAAAA
                     DLQLRHVVVTSVTRDDLPDGGAGHFADVVAALRARLPAAAVEILTPDFRGVAGAVDII
                     IAARPDVFNHNVETVPRLYPAVRPGADYARSLALLAEVGRRSPIAVKSGLMVGLGETR
                     EELRTVFHDLAGAGVRLLTIGQYLAPSAAHHPVVRYLPPGEFAELRQEAEAAGIPLVA
                     AAPLVRSSYRADEFCR"
     gene            2782..>2964
                     /locus_tag="PK112_04025"
     CDS             2782..>2964
                     /locus_tag="PK112_04025"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPC11250.1"
                     /translation="MAERPTTHIVVIDDEQYICNIIEEALAAPEFQVAAFSDPLAALA
                     HIDDHPVDLVLTDLVMG"
BASE COUNT          503 a         1021 c          945 g          495 t
ORIGIN      
        1 ccgataccat gagatccacc gggcgccacg cgccgccgac aaacacctcc gcggtcccca
       61 tgttgtgacg aaggctcacc ggcgccgccg tcccggtgag cgggtccatg atcctgacca
      121 gataggttcc atccacctgg agccatcccg agaacccgtc aaccaccacc cactgccccg
      181 gtgtgccggc cagtcccagc agcaccgacc gctcccagtt ctccgcccag tcgcggatct
      241 ggaaatagtt ggggcggcgc cgataggcgg tgtagaggtc gtcccccttg acgtgcaggt
      301 attcattgag cccggcgaga aacccctcgt cccaggtgcc ggtgttgacc gtggtttcca
      361 tccagtaggc caggcgggcc gccagggtgt ccagcgacaa cttgaggttg ttctcggtca
      421 tcggccgcgt cgtcccccgg tcgtaccaca gctcgatggc cgcggccgcc gcgtaagggg
      481 cattccagtg cggaccgcgc gcgggcgggc cggcgacaat gacgccggcc gagtactcat
      541 cgagcgcccc gcgtcggaac ccctgggtgt agtcgacgtc ctcatccgag cagacgaggt
      601 tgaacgtcag gggcgtgcag aaactcccgc cgtcctcatg cgagatcacc gtcggcaccg
      661 gcggcgtcgg ttcaaggtag accgtgacgc tgtcgcggtc gaagcggccg agggtgtcgt
      721 agaccgtcgc gcgcagcgtc cacatcccct cggtgagcgc gccgaaattg aacgcgtacg
      781 acaatccggc ggcgctggcc gccgtggccg aggccccgtc gcggaaggcc gcggtggggt
      841 caaaatcccg accgatctcg acgtaggctc cgccgccggc gggacggtac tcgaacaccg
      901 cataatcgag aatgggcgaa ccgcccttct cctggatcca gatctcggtc gggccgaaca
      961 gggtgtcgga tggtctcggg ctgaggagcc gcacgtccgg cggattggcc gtcccgatcg
     1021 gcacgccggc cgcgtacaac acgaggcggc ccgggaaatt ccagatttcg ttgtcgccca
     1081 gatcgatgaa gcccaccgcg gtgtcccaga tgttgtacga gacgcacagc tccgggatcg
     1141 tgtcgatcac caccgccgcg ccgtcggcct ccgcgaacgg cgtgctgagg aagaacccgg
     1201 caaagaacgg cccggttacc gtcaccggcg tgtccagcgg tatccagatg tcgtagagac
     1261 cctcggccgg aacctgcagc gtgtactccg acgaaatcgc caccagcggt cccggcatcg
     1321 ggcaggacgg atccgagttg tccgccagct caacatccac ggacacgccg aaagtcggcg
     1381 tcccggtgaa atacatcggc atattgattt cggtcacgat aaacgggtag gggttggggc
     1441 aggtgagcgc cgggtcgcag taccccttgt acaactcgtt gccggtgacc cagccgtcga
     1501 tcttatagta aatgccggcg tcgtggcgaa cgatgcaggt atctgcggtg gccagactgg
     1561 ggacgggacg gagttcggcg cgggcagcgc cgtcgggaac cggcaccagt cgggcctggc
     1621 cgcgttcggc gcgcggactg ctggccagaa ccggaaggag cagacacccg acaaccacaa
     1681 gcagacgacg cataggctct cctttgcggt gtttgcacac gtctacttga attatcgtgc
     1741 aaatttcggt atacttgatt ggtaaagaac tgattgcggc gaaacctatg gcagactccc
     1801 caccctcccg cccccccgga ggcggtccgc gcaagcccgc ctggctccgc gtaaccgcct
     1861 tctccggaat gggttacgaa aacgtctccc ggctcctgcg ccactatggg ctcaacacgg
     1921 tctgtcatgc cgcgaattgc cccaaccgcg gcgagtgttt taaccgcggc accgccacct
     1981 tcctcatcat ggggcccacc tgcacgcgca actgccgctt ctgcgacatc gagccggggc
     2041 gcccgcgccc tctcgatccc accgagccgg cccgcgtcgc cgccgcggcg gccgacctgc
     2101 agctccgcca cgtggtcgtc acctcggtca cgcgcgacga ccttcccgac ggcggcgccg
     2161 gtcatttcgc cgatgtggtc gcggcgctcc gcgcgcggct ccccgcggcg gcggtggaaa
     2221 tcctcacgcc cgatttccgg ggcgttgccg gcgcggtcga catcatcatc gccgcccgcc
     2281 ccgacgtctt caatcataac gtcgagaccg tgccccggct ctacccggca gtgcgccccg
     2341 gcgcggacta cgcgcgatcg ctggcgctgc tggccgaagt ggggcgccgc tcccctattg
     2401 ctgtcaagtc gggcctcatg gtcgggctgg gggagacccg cgaggaactt cggacggtct
     2461 tccacgatct cgccggcgcc ggcgtgcgcc tgctgacgat tggccagtac ctcgctccgt
     2521 cggcggccca ccacccggtc gtccggtacc ttccgcccgg cgaattcgcc gaactgcggc
     2581 aggaggctga ggcggcgggg atcccgctgg tggcggctgc cccgctggtc cgctcctcct
     2641 atcgagctga cgaattctgt cggtgactgt cctccgcttg ctcagcgcaa aaataaaaca
     2701 ttgtcggcgg gcggctgtgc cttttgcccc gttgtccgat aacagaggag aagatccgct
     2761 gaaactgtaa agagggcatc catggctgag agaccgacga cccacattgt ggtgatcgac
     2821 gacgagcagt acatctgcaa catcatcgag gaggccctgg cggctccgga attccaggtc
     2881 gcggcgttct ccgatccgct cgcggcgctt gcccacatcg acgatcaccc ggtcgatctg
     2941 gtcctgaccg atctggtgat gggg
//
LOCUS       DAOOKS010000193         2960 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_40728_length_2960_cov_2.724613, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000193 DAOOKS010000000
VERSION     DAOOKS010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40728_length_2960_cov_2.724613"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            36..677
                     /locus_tag="PK112_04030"
     CDS             36..677
                     /locus_tag="PK112_04030"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HPC11251.1"
                     /translation="MIPFRVKVCGITRPEDAAVAVTNGADLIGLIFYSRSPRRVSPDA
                     AAAIITKLPPVVHRVGVFVDDPLDRVLATAARLDLDFLQLHGPYTDDDVRRARRVGFR
                     TIRLRTVRRPSDYAAFYTDPADIVMVDHPSGALAGGTGTAFDWRIRPPVPIVNLMLAG
                     GITADNLAEGVRTFQPLVVDVNSGVEEAPGVKSAEKMKRFFEVANRLRYAKAD"
     gene            661..1863
                     /gene="trpB"
                     /locus_tag="PK112_04035"
     CDS             661..1863
                     /gene="trpB"
                     /locus_tag="PK112_04035"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HPC11252.1"
                     /translation="MPRRTRRQPDRRGRFGPYGGRYVPETVMAALDDLERAYRGIART
                     PEFRRELGGHLRDFVGRPTPLYFAERFSERVGRAVYFKREDLNHTGAHKINNVLGQIL
                     LARRMGKRRVIAETGAGQHGLATAAACARFGLTCIVYMGAEDIVRQKPNVEKMALLGA
                     AVVPVESGSRTLKDATSEALRDWVTNVRDTFYVIGSVVGPHPYPMIVRDFQSVIGRET
                     LAQCRRRFGALPDALVACVGGGSNAIGLFAPFLRHDAVRLFGVEAAGLGLRSGRHAAT
                     LTRGKPGVLHGTYSYLLYDRDGQVVLPHSVSAGLDYPGVGPEHAFLKDAGRVEYVAVT
                     DREALAAFRLVSRLEGIIPALETAHALAWMVKAARRFGKSDRIVVNLSGRGDKDMAIV
                     GKAGKTFR"
     gene            1860..2651
                     /gene="trpA"
                     /locus_tag="PK112_04040"
     CDS             1860..2651
                     /gene="trpA"
                     /locus_tag="PK112_04040"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HPC11253.1"
                     /translation="MSRVSRLLSARRPGHKLLAPFFTAGFPDPAVWRALVGTAAAAGA
                     DIIEIGIPFSDPLADGPAIQFSSHCALRNGLSLAVLLDGIADLRARLDLPFILMGYYN
                     PLLAYGEERFLEDAAAAGVDGLIIPDLPIEEAVSYRRAARRQGLSTVFLVAPTSTPER
                     VKLIDKSSTDFVYAVTVTGVTGTGKTFGEATDAYLRRLTAVLTRPFVTGFGVNGPAAA
                     RRMARYADGVVIGSALVEVIRSAATKRQAATEVGRFLDTVRRVLP"
     gene            2648..2938
                     /locus_tag="PK112_04045"
     CDS             2648..2938
                     /locus_tag="PK112_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HPC11254.1"
                     /translation="MTAKGIDRLRDQIDALDRRLLSLLNERAGLVLDLARLKRADRLP
                     VFDPAREKQILEQVVGQNRGPLPGDAVARLFERIIDESRRLERTEVYDTPSE"
BASE COUNT          449 a         1016 c          973 g          522 t
ORIGIN      
        1 tcgcgcgacg atctcctgca ggagttgcgc ggcgtatgat tccgtttcgc gtgaaagtgt
       61 gcggtataac ccgccccgag gacgccgcgg tggcggtcac caacggggcc gacctgatcg
      121 gcctgatttt ctactcccgc tccccgcgcc gggtcagccc cgatgcggcc gcggccatta
      181 tcacgaagct cccgccggtc gtgcaccgcg tcggcgtgtt tgtcgatgac cctcttgacc
      241 gggttctcgc caccgccgcg cggctcgatc tcgatttcct tcagctccac ggaccgtata
      301 ccgatgacga cgttcgcagg gcgcggcgcg tgggtttccg gacgatccgc ctgcgcacgg
      361 tccggcgccc gtccgactac gcggccttct acaccgaccc ggccgacatc gtcatggtcg
      421 accacccgtc tggcgccctt gccggcggca ccgggaccgc tttcgactgg cgcatccgcc
      481 cgcccgtgcc gattgtcaat ctcatgctgg ccggaggcat cacggccgac aacctggccg
      541 agggagtgcg gacgttccaa ccgctcgtgg tggatgtgaa ctccggggtc gaggaggcgc
      601 ccggcgtcaa atcggcggag aagatgaaac gattctttga ggtcgccaac cggctacgct
      661 atgccaaggc ggactaggcg acagcccgat cggcgcgggc ggtttggccc ctacggcggc
      721 cgctatgtcc ccgaaaccgt catggccgcg ctcgatgacc tcgagcgggc ctaccggggg
      781 attgcgcgca cgccggagtt tcggcgcgag ctgggcgggc acctccgcga ttttgtcggg
      841 cggccgaccc cgctgtattt tgcggaacgg ttctccgagc gggtcggccg tgcggtttat
      901 ttcaagcgcg aagacctcaa tcacacgggc gcccacaaga tcaacaacgt gctcgggcag
      961 atcctcctgg cccggcgcat gggcaagcgg cgagtgatcg ccgaaaccgg cgccggccag
     1021 cacggcctgg cgaccgccgc cgcgtgcgct cgtttcggcc tgacctgcat cgtctacatg
     1081 ggggccgagg atatcgtccg ccagaaaccc aacgtcgaga aaatggctct cctcggggcg
     1141 gccgtcgtcc cggtggagag cggctcccgg accctgaagg acgccacgag cgaggccctg
     1201 cgcgactggg tcaccaacgt gcgcgacacg ttttatgtca tcggctcagt cgtcggcccg
     1261 cacccgtacc cgatgatcgt gcgcgatttc cagtcggtca tcgggcgcga gaccctcgcc
     1321 cagtgccgcc ggagatttgg ggcccttccc gacgccctcg tggcctgcgt cggcggcggc
     1381 tcgaacgcga tcggcctctt cgccccgttt ctgcgccacg acgctgttcg ccttttcggt
     1441 gtggaggcgg ccggcctcgg gctgcgttcc gggcgccacg ccgccaccct cacccgcgga
     1501 aaacccggcg tgctgcacgg gacctacagt tacctcctgt acgaccgaga cggccaggtc
     1561 gttctgccgc attcggtatc ggccggactg gactaccccg gtgtcggtcc ggagcatgcc
     1621 ttcctcaagg atgcgggccg ggtggagtat gttgcggtga ccgatcgcga ggctctcgcc
     1681 gcctttcgcc tggtgagccg tctggaaggc atcatcccgg ccctggagac ggctcatgcg
     1741 ctggcctgga tggtcaaagc ggcccgccgg ttcggaaaat ccgaccggat cgtggtcaac
     1801 ctctccggcc ggggcgacaa ggacatggcg attgtgggga aagcggggaa gaccttccga
     1861 tgagtcgcgt gtcccgcctg ttgtcggccc gacggcccgg gcacaaactg ctggcgccgt
     1921 ttttcaccgc cggctttccc gacccggcgg tgtggcgggc gctggtcggc acggccgccg
     1981 cggcgggtgc ggacataatc gagatcggca tacccttctc cgacccgctg gccgacggcc
     2041 cggcgatcca gttctcctct cattgcgccc tccggaacgg cctcagcctc gccgttttgc
     2101 tcgacggcat cgccgacctg cgggcgcggc tggatctccc gttcatcctc atgggctact
     2161 acaatcccct cctcgcgtac ggcgaggagc ggtttctgga ggatgcggcc gcggccgggg
     2221 tcgatggcct gatcattccc gatcttccaa tcgaagaagc ggtgtcctac cgccgagcgg
     2281 cgcggcggca gggtctgtcg accgtctttt tggtcgcccc cacctcgact cccgaacgcg
     2341 taaaactgat tgacaaatca tcgaccgatt ttgtatatgc tgtgaccgtg accggggtca
     2401 ccggcacggg gaagaccttt ggcgaagcga ccgacgcgta tctgcggcgg ctgacggcgg
     2461 ttctgacccg gccgttcgtc accgggttcg gcgtcaacgg cccggccgcc gcgcggcgga
     2521 tggcgcgcta tgccgacggc gtcgtcatcg gctcggccct ggtcgaagtg atccgctcgg
     2581 ccgccacgaa acggcaagcg gcgaccgagg tgggccggtt tctggacacc gtgaggcgcg
     2641 ttctcccatg acggcgaaag gtatcgacag actgcgcgat cagattgacg ccctcgaccg
     2701 gcggcttctc agcctgctca acgagcgggc cgggctggtg ctcgatttgg cccggctcaa
     2761 gcgggccgac aggctgccgg tgtttgaccc ggcccgagag aaacagatcc tcgaacaggt
     2821 cgtcggtcaa aaccgcggcc ccctgcccgg cgacgcagtc gcacggctgt ttgaacggat
     2881 tatcgatgaa tcccgccgcc tcgaacgcac cgaagtgtac gacacgccgt cggaatgaaa
     2941 gagaccccat gatcgtcatc
//
LOCUS       DAOOKS010000194         2956 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_40802_length_2956_cov_3.288176, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000194 DAOOKS010000000
VERSION     DAOOKS010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40802_length_2956_cov_3.288176"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="PK112_04050"
     CDS             complement(<1..607)
                     /locus_tag="PK112_04050"
                     /inference="COORDINATES: protein motif:HMM:NF026199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family insertase periplasmic-domain
                     containing protein"
                     /protein_id="HPC11255.1"
                     /translation="MDKKTIIIVVILGAIIIFYYPILQFLGLYEPPRPSSPGTAPDST
                     AAVDSSAPVPVIKPEAVQEVTVKPQPVAEIPADTVGADTIAVETEFYRILLSTRGGGP
                     VSIQLKEYTYIGGANVEMLPECEAATPNAVFAGGTFSTAALPFQASLAPGRYSVARET
                     LPISFTYTNPGGGIIRKTYFFYPDQYHYDLTLTVEDTRALGF"
     gene            complement(649..945)
                     /gene="yidD"
                     /locus_tag="PK112_04055"
     CDS             complement(649..945)
                     /gene="yidD"
                     /locus_tag="PK112_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017595549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HPC11256.1"
                     /translation="MRNSVVYSRRSIATPTNPRPSVAARLLILLVRIYQATLSGLFAG
                     SCRFYPSCSHYSVEALRSHGALSGLYLSVRRLLRCHPWHPGGYDPVPSAKETRE"
     gene            complement(896..1261)
                     /locus_tag="PK112_04060"
     CDS             complement(896..1261)
                     /locus_tag="PK112_04060"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030677 - ribonuclease P complex
                     [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_function: GO:0042781 - 3'-tRNA processing
                     endoribonuclease activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HPC11257.1"
                     /translation="MPARSNQSSRCLPRCLSLKQRRAIEDVVQRGRTLRGAAATFRWQ
                     PADRFGYVVVVGRRHGHAARRNRIKRLLREALRLNRHRLTREVRIAVLPRLYGDALRF
                     DILHAEFGRIFEAINRHAD"
     gene            complement(1245..1397)
                     /gene="rpmH"
                     /locus_tag="PK112_04065"
     CDS             complement(1245..1397)
                     /gene="rpmH"
                     /locus_tag="PK112_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018674684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HPC11258.1"
                     /translation="MKRTFQPSNRKKLNDHGFRKRMASKGGRLVLSRRRAKGRKRLTV
                     KVARKK"
     gene            1631..>2956
                     /locus_tag="PK112_04070"
     CDS             1631..>2956
                     /locus_tag="PK112_04070"
                     /inference="COORDINATES: protein motif:HMM:NF018530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HPC11259.1"
                     /translation="MASILNLLAQNDLLLLFVIIGLGYLIGNIRILGFKLGVAAVLFV
                     GIAFSALDKRLILPEQIYIVGLVLFVYAIGLQAGPGFFTSFHRRGLRISAIAIVILTA
                     GAVVAGVIGHFLNLPATSIAGLYCGALTNTPALAAAVEATANMAKALPPDKVQFYLNS
                     PVVTYGLAYPIGVAGMILWMFAFAKIYRVDFSRETEEVKREADAEAIASRTFRVTNPA
                     VQGQTVEQFFAAFEQPQFVFSRIKKGDLVQVVSPETVLEANDLIVAVGPSKALRTAHL
                     LLGSESNETLAERRDETAYKWFFVSNPLAVGRSISELRRQRAYVGTVTRLRRGDVEFV
                     PSPTTTLEMGDRVLVVSPRLEVDRIAGYFGDSVKALSETDYLSLSAGIVLGVFLGMIP
                     IPLPNGTAFKLGFAGGPLVAGLVLGRLGRTGPIHWSLPFNANLALRQIGL"
BASE COUNT          516 a          940 c          923 g          577 t
ORIGIN      
        1 cgaaaccgag cgcccgtgtg tcctccaccg tgagcgtcag gtcgtagtgg tactggtccg
       61 gatagaagaa ataggtcttc cggataatcc cgcccccggg gttcgtgtaa gtgaacgata
      121 tcggcagcgt ctcgcgcgcg acgctgtacc ggcccggcgc gagactcgcc tgaaacggaa
      181 gggccgctgt cgagaatgtc ccgccggcga aaaccgcgtt cggggtggcg gcttcgcact
      241 ccgggagcat ttccacattc gcgccgccga tgtacgtgta ctccttcaac tgaatcgaca
      301 ccgggccacc cccgcgcgtc gatagaagaa tccggtagaa ctccgtttct accgcgatcg
      361 tatccgcccc gacggtgtcc gccgggatct ccgccaccgg ctgcggtttc acagtaactt
      421 cctgcaccgc ttccggtttt atcacgggaa caggggcgga agagtcaacc gccgcggtag
      481 aatccggtgc tgtcccgggg gacgacggtc gcggcggctc gtacaacccg agaaactgaa
      541 gaatcggata gtagaaaatg ataatcgcgc caagaatgac gacgatgatg atggtttttt
      601 tgtccacggt gacccttggt ttcagtcttt atcccggagc cggccggctt attcacgggt
      661 ttctttcgcc gacggcaccg gatcgtagcc tcccgggtgc cacggatgac atctaagtaa
      721 tcgcctgaca gagagataca atccgctgag agctccgtgt gaacgaagcg cctcgactga
      781 gtagtgcgag caactgggat agaagcggca acttccggca aaaagcccgg agagcgtcgc
      841 ctggtatatg cgaacgagca aaatcagaag acgggcggcc acagatggcc gggggttagt
      901 cggcgtggcg attgatcgcc tcgaatatac gaccgaattc cgcatgcaaa atgtcaaacc
      961 gcagggcgtc tccataaagc cggggcagca ccgcgattcg gacctcccga gtgagacggt
     1021 ggcgattcag tctgagcgcc tcgcgcagga ggcgcttgat ccggttacgc cgggcggcat
     1081 ggccgtggcg gcgtccgacg acgacgacat atccgaaacg gtcggccggc tgccagcgga
     1141 atgtcgccgc cgcgccgcgc agcgtgcgcc cgcgctgcac cacatcctcg atcgcccggc
     1201 gctgcttgag actcaggcag cgaggcagac agcgggacga ctggttactt cttgcgggca
     1261 accttgacag tcagacgctt tcttcccttg gcgcgacggc gcgagaggac gaggcgccct
     1321 cccttggagg ccatgcgctt gcggaagcca tggtcgttaa gtttcttccg gttcgacggc
     1381 tggaatgtcc gtttcatttc ttccttacct caagtcggtt aaactttcga atataaccaa
     1441 tccccccgac tcgtcaaggg attaatgtgc tcgcgggggc cccggcggcc gcgggctgcg
     1501 ccccggtggg cacgtcgtat ggtccggccg gcggcgcgtc aggtgcctgt ccgcttttta
     1561 gttgcgcccc ggcacggcgg tgggtatctc gtgaaacaca ggccgtgcgg cggtgtgaaa
     1621 ggctgaagta atggcgtcga ttctgaacct gcttgcccag aacgacctcc tcctgctctt
     1681 cgtgattatc gggctgggct acctgatcgg gaacatccgc atcctcggtt tcaaactggg
     1741 ggtggccgcc gtgctgttcg ttggtattgc cttctccgcc ctcgacaaac gcctcatcct
     1801 tcccgagcag atttacatcg tcgggctcgt tctgttcgtc tatgcgatcg gcctgcaggc
     1861 cggcccggga tttttcacct cgttccaccg gagggggctg cgcatcagcg ccatcgccat
     1921 cgtcatcctg acggccggcg ccgtggtcgc cggggtgatc ggccattttc tcaatctccc
     1981 ggccacaagc attgccggcc tctactgcgg ggcgctcacc aacaccccgg ctctcgccgc
     2041 ggccgtcgaa gccaccgcca acatggccaa agcccttccc ccggacaaag tccagttcta
     2101 cctcaacagc ccggtcgtga cctacggtct ggcctaccct atcggcgttg ccgggatgat
     2161 tctctggatg ttcgcgtttg cgaagattta ccgcgtggat ttctcgcgcg agaccgagga
     2221 agtcaagcgc gaggcggatg cggaagcgat cgcgagccgg acgttccggg tcaccaatcc
     2281 cgccgtccag ggacagaccg tcgagcagtt cttcgccgct ttcgaacagc cgcagtttgt
     2341 tttcagccgc atcaagaagg gggacctggt gcaagtggtg agcccggaga ccgtgctcga
     2401 ggccaacgac ctcatcgtcg ccgtcggccc ctcgaaagcc cttcgcactg cccacctcct
     2461 gctcggcagc gaatcgaacg agacgctggc ggaacggcgg gatgagacgg cgtacaagtg
     2521 gttcttcgtc tcgaatccct tggcggtggg ccggtcgatc agcgagctca ggcgccagcg
     2581 cgcctatgtc gggacggtca cgcgcctgcg ccggggcgac gtcgagtttg tcccgtcccc
     2641 gaccacaacg ctggagatgg gcgaccgcgt gctggtcgtg tcgccgcgcc tcgaggtcga
     2701 ccgcatcgcc ggctatttcg gcgattccgt caaagcgctc tccgagaccg attacctgtc
     2761 gctctcggcg gggatcgtcc tgggggtgtt tctcggcatg attccgattc cgctgcccaa
     2821 tgggacggcg ttcaagctcg gctttgcggg cgggccgctg gtggccggcc tggttctcgg
     2881 ccgcctcggc cgcaccggac cgatccactg gagcctcccg ttcaatgcca acctcgcgct
     2941 gcggcagatc gggctg
//
LOCUS       DAOOKS010000195         2936 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_41198_length_2936_cov_3.714682, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000195 DAOOKS010000000
VERSION     DAOOKS010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41198_length_2936_cov_3.714682"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="PK112_04075"
     CDS             <1..201
                     /locus_tag="PK112_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007219967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPC11260.1"
                     /translation="GGSIRPSPYSRYRQWMTHKLASWVDQFGTFGCVGCGRCITWCPV
                     GIDITEEARVFAHSLQRAEAHT"
     gene            216..674
                     /locus_tag="PK112_04080"
     CDS             216..674
                     /locus_tag="PK112_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HPC11261.1"
                     /translation="METLEKILSEHSFFKDLSPAHNAIICGCAANTVFPKGEFLFREG
                     EAADKFYVIRQGKVLVETHVPHRGPVAIQSREAGEVLGWSWLVPPYRWHFDARAVETT
                     RAIVFDGKCLRERFASDHELGYEVMRRFVTVIAERLDATRMQLLDVYGHH"
     gene            661..1530
                     /locus_tag="PK112_04085"
     CDS             661..1530
                     /locus_tag="PK112_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HPC11262.1"
                     /translation="MAITKTNAAAESPALLDPMLGQPYRIGRVVKETYDTFTLELAPT
                     EEGNGFAFLPGQFNMLYVYGVGEVPISISGDPAKPRALIHTTRAVGSVTKQMQKLRKG
                     DVIGVRGPYGTAWPVEEAKGRDVVVVAGGIGLAPLRPALYHLLKHRKRYGKVILLYGA
                     RTPDDVLYPKMLAQWGARPEIEVLTTVDRGMGQWTGNVGVVTTLVPKVTFDAANCLAM
                     ACGPEVMMRFTALALSKRGLADEQIYVSMERNMKCGIGLCGHCQTGSLFVCKDGPVFR
                     LDRVKPLFTQREI"
     gene            1535..2314
                     /locus_tag="PK112_04090"
     CDS             1535..2314
                     /locus_tag="PK112_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HPC11263.1"
                     /translation="MAKSVKPKLAVWKFASCDGCQLSLLELEDELLAITGAVEIANFL
                     EASREVVPGPYDVSLVEGSITTPHDAERIHKVRRASKLLIAIGACATSGGIQALRNFA
                     EIRTYIPAVYARPEYIETLRKSTAIADHVHVDFELRGCPISKYQLLEVVNALLNGRKP
                     NVPPYSVCLECKRRGTVCVMVSQGLACLGPVTQAGCDALCPSYNRGCFGCFGPKETPN
                     PSSLANWFKEQQMSSDDIVRTFRQYNAYAEPFRKESEAHEK"
     gene            2304..>2936
                     /locus_tag="PK112_04095"
     CDS             2304..>2936
                     /locus_tag="PK112_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005000139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HPC11264.1"
                     /translation="MKNRTITVDMLARVEGEGGLTVRVKQDKVTEVRFNIFEPPRFFE
                     AFLRNRRFSEAPDITARICGICPVPYQMSSAQAMEAICGAAVEGPLRDLRRLLFLGEW
                     IESHALHVYLLHAPDFLGYASGIHMAADYPDIVKRGLRLKKIGNDVMVLLGGREIHPI
                     NIRTGGFYSVPSRAAVLALKKDLEWALEASLETVKWTAGLSFPDFEPDYEM"
BASE COUNT          529 a          932 c          979 g          496 t
ORIGIN      
        1 ggcgggagca tccggccctc gccctactcc cggtaccggc agtggatgac gcacaagctg
       61 gcctcgtggg tcgatcagtt cggcaccttc ggatgcgtcg gctgcggccg ctgtatcacc
      121 tggtgcccgg tgggcataga tataacggaa gaagcccgag tattcgcaca cagcctccag
      181 cgcgcggaag cgcatacctg agtcaggagg aacctatgga gacccttgag aaaattctgt
      241 cggagcactc cttcttcaaa gacctctcgc ctgcgcacaa cgcgattatc tgcgggtgtg
      301 ccgccaacac ggtgttcccg aaaggggagt ttctgttccg ggagggcgaa gcggcggaca
      361 agttctacgt gatccgccag ggcaaagtgc tggtggagac gcacgtgccg caccgcggac
      421 cggtagccat tcagagccgg gaggcgggcg aggtgctggg gtggtcctgg ctggtgcctc
      481 cctaccggtg gcattttgac gcgcgggcgg tcgaaacaac gcgggcgatc gtgttcgacg
      541 gcaagtgcct gcgcgagagg ttcgccagcg accatgaact gggatacgaa gtcatgcgcc
      601 ggttcgtcac cgtcatcgcc gaacggctcg acgccacgcg catgcagctc ctggatgtat
      661 atggccatca ctaagacaaa tgccgcagcc gagtctccgg ccctcctcga tcccatgctc
      721 gggcagccct atcgcatcgg gcgggtggtc aaggagacct acgatacctt tacgctggag
      781 ctggcgccga ccgaggaggg gaacgggttc gccttcctcc ccggtcagtt caacatgctc
      841 tatgtctacg gggtgggaga agtcccgata tcgatatccg gcgatccggc caaaccccgg
      901 gccctcattc acacgacgcg cgcggtcggg tcggtcacga aacagatgca gaagctgcgc
      961 aagggcgacg tgatcggggt gcgcggtccc tacggcaccg cctggccggt cgaggaggcg
     1021 aaggggcgcg acgtggtggt ggtggcgggc gggatcggac tggcgccgct gcgccccgcg
     1081 ctctaccatc tcctcaagca ccgcaagcgg tacggcaagg tgatcctgct gtacggcgcg
     1141 cggacgccgg acgacgtgct ctatccgaaa atgctcgcgc agtggggagc ccggcccgag
     1201 atcgaggtgc tgaccacggt tgaccgcggc atggggcagt ggacggggaa tgtcggcgtg
     1261 gtgacgacgc tggttccgaa ggtgacgttc gacgcggcca actgcctggc gatggcgtgc
     1321 gggccggagg tgatgatgcg gttcacggcg ctggcgctgt ccaagcgcgg gctggctgac
     1381 gagcagatct acgtgtcgat ggagcgcaac atgaaatgcg gaatcggcct gtgcggccac
     1441 tgccagacgg ggtcgctgtt cgtgtgcaag gacgggccgg tgttccgcct ggaccgcgta
     1501 aaaccgctct ttacgcagcg ggagatatga cattatggcg aagtcagtca aaccgaaact
     1561 cgcagtgtgg aagttcgcct cgtgcgacgg ctgccagctg agtctgctgg aactcgagga
     1621 tgaactgctg gcgataaccg gggcggtgga gatcgccaac ttcctcgagg cctcgcgcga
     1681 agtggtcccc gggccctacg atgtatcgct ggtcgagggg tcgatcacga cgccccacga
     1741 cgccgaacgg atccacaagg tgcgccgggc atcgaaactg ctcattgcca tcggcgcctg
     1801 cgcgacctcc ggcgggatcc aggcgctccg caatttcgct gaaatccgca cctacatccc
     1861 ggccgtctac gcgcgcccgg agtatatcga aacgctgcgc aagtcgaccg cgatcgccga
     1921 ccacgtccac gtcgatttcg agctccgcgg ctgcccgatc agcaagtacc agctcctcga
     1981 ggtcgtcaat gccctcctca acggccgcaa gcccaacgtc cccccctact ccgtctgcct
     2041 cgagtgcaag cggcgcggga ccgtctgcgt catggtctcc caagggctcg cctgcctcgg
     2101 cccggtcacc caggccgggt gcgacgccct ctgcccgagc tacaaccgcg gctgcttcgg
     2161 ctgtttcgga ccgaaggaga cccccaatcc gtcgtcgctg gcgaactggt tcaaggagca
     2221 gcagatgtcg agcgacgaca tagtccgcac gttccgccag tacaacgcgt acgcggagcc
     2281 attccggaaa gagagcgaag cgcatgaaaa atagaacgat cacggtggac atgctcgcgc
     2341 gggtcgaggg ggagggcggg ctgacggtcc gggtcaagca ggacaaggtg accgaggtcc
     2401 gcttcaacat cttcgagccc ccgcggttct tcgaagcctt cctccgcaac cggcggttct
     2461 ccgaggcgcc ggacatcacc gcccgcatct gcggcatctg cccggtgccc taccagatga
     2521 gctcggccca ggcgatggag gcgatctgcg gggcggccgt cgagggtccg ctgcgcgatc
     2581 tgcgccgcct cttgttcctc ggcgagtgga tcgagtcgca cgctctccac gtctacctgc
     2641 tgcacgcccc cgacttcctt ggctacgcga gcggcatcca catggccgcc gactaccccg
     2701 acatcgtcaa gcgcgggctg cgcctgaaga agatcggcaa cgacgtcatg gtgctgctgg
     2761 gcgggcggga aatccacccc atcaatatcc gcaccggtgg gttctacagc gtgccctcgc
     2821 gggcggccgt gctcgcgctc aagaaggatc tcgagtgggc gctggaagcc tcgctggaga
     2881 cggtcaagtg gacggccggg ttgtcgttcc cggattttga gccggactac gagatg
//
LOCUS       DAOOKS010000196         2928 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_41376_length_2928_cov_3.045249, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000196 DAOOKS010000000
VERSION     DAOOKS010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41376_length_2928_cov_3.045249"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="PK112_04100"
     CDS             complement(<1..283)
                     /locus_tag="PK112_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11265.1"
                     /translation="MSKKELHSKLDRLRGHIVAKPAAPPPEVLPERCRVLAERLDGEA
                     VCTARGGFVLIRKRFPPVYTHGEIALADFAARTDFPASAFTASDDPACLP"
     gene            complement(258..1199)
                     /locus_tag="PK112_04105"
     CDS             complement(258..1199)
                     /locus_tag="PK112_04105"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HPC11266.1"
                     /translation="MRTLIGGLLLVIVLAPALSAQIDLQHRIDSLFVIASSGEVRYQK
                     LTGPAMDSIAAFGQPAVPYLIDKFDTKSARDRWTIIWILQRIGSPAVPDLVRALDRAD
                     PLVVSRVAWALGDIKDTGAVLPLIAKSTHAAWQVREETIGSLGDIGDRRGDNAILAAL
                     GDSIGQVRKAAAVAAGRLKILEAAAPLVHRFGDPFYGARLAAVDALLQLDTVLSLPAL
                     IDSLASADAFVSALSAAALGRIGADRALDALFRQATEDPDPARRVPAACALIEADPLD
                     RCRYREKLLAAQPDRLGRLKVESALKTARHVEKGTAQ"
     gene            complement(1316..>2928)
                     /locus_tag="PK112_04110"
     CDS             complement(1316..>2928)
                     /locus_tag="PK112_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11267.1"
                     /translation="TGPEMQQGYNLFYASPGLNQLMARAGLYLDVIFPRSAETALASL
                     SPAQRRFLTAEFKELLVSHAEEEFFTPEQLDSVEKIEEKYTEEFTAFGWRIDKDPILT
                     AGFDCLRDLMPEIAALRRDLAAGTLDAGAVMKGLGQIPEKGVGEESYLGRQPGWKVGG
                     PGPDYYEGDYWFIFDFGGDDVYRLSYDPARPHGVIIIDLAGDDRYRGLTDFALASGCL
                     SAGLLLDFGGNDRYDAQSFALGSGYFGFGLLYDAAGDDRYDGDTHVMAAGSFGLGLLI
                     DEGGRDCYNAALYAQGFGFTEGMGVIYDTDGSDSYYAGGKYKDILRYEDHYLSLSQGF
                     GYGLRPWTSGGIGAIIDDRGSDAYYTDIFGQGTSYWWSLGVIYDSSGNDTYNSFQYAQ
                     GCATHMTLGVLIDDAGNDAYIGKGLMQGVGHDYSCGLLLDRAGDDLYTAWDLSQAAGS
                     ANGVGILIDNAGADRYFIRNADNTHGYGNPRRDFGSIGLFLDLGGEDLYNGYGGDNLY
                     WRTPSQWGGGMDIERAPRPDSAVIGEVQ"
BASE COUNT          475 a          872 c         1137 g          444 t
ORIGIN      
        1 cggggaggca ggccgggtcg tcggaggcgg tgaaggcgga ggcggggaag tcggtgcggg
       61 cggcgaaatc cgccagcgcg atctcgccgt gggtgtagac agggggaaag cgtttgcgga
      121 tgaggacaaa accgccgcgc gcggtgcaga ccgcctcgcc atcgagccgc tcggccagca
      181 cccggcagcg ctccgggaga acctcgggcg gcggggccgc gggttttgcg acgatgtggc
      241 cgcggagccg gtcgagctta ctgtgcagtt cctttttcga catggcgggc cgttttcagg
      301 gcggactcca ctttcaggcg cccgaggcgg tcaggctgcg cggcgagcag tttctcgcgg
      361 tagcggcagc gatcgagcgg gtcggcctcg atgagggcgc aggcggccgg cacgcgccgg
      421 gccgggtcgg gatcctccgt cgcctgccgg aaaagcgcgt cgagcgcgcg gtcggcgccg
      481 atgcggccga gggccgcggc cgagagggcg ctcacgaagg cgtcggccga ggccagagaa
      541 tcgatgaggg cgggcaggct gagcacggtg tccagctgca ggagggcgtc gaccgcggcc
      601 aggcgggcgc cgtagaaggg atcgccgaac cggtggacca ggggcgcggc ggcctcgagg
      661 attttcagcc tgccggcggc gaccgcagcc gccttgcgca cctggccgat cgagtcgccc
      721 aaggcggcca ggatggcgtt gtcgccgcgc cggtcgccga tgtcgccgag agagccgatc
      781 gtctcctcgc ggacctgcca ggcggcgtgg gtgctcttgg cgatgagcgg gagaacggcg
      841 ccggtgtcct tgatgtcgcc gagcgcccag gcgacgcgcg agaccacgag cggatcggcg
      901 cgatcgagag cccgcaccag gtcgggaacc gcgggagagc cgatgcgctg gagaatccag
      961 atgatcgtcc agcggtcgcg ggccgatttc gtgtcgaact tgtcgatcag gtatgggacc
     1021 gccggctggc caaaagccgc gatcgagtcc atcgcagggc cggtcaactt ctggtagcgc
     1081 acctcgccgg aggaagcgat gacaaacagc gagtcgatgc ggtgctggag gtcgatctgc
     1141 gcgctcagag cgggggcaag gacgatcacc aacaggaggc cgccgatgag ggtgcgcacc
     1201 cggccgccgg gccgccacag cggccgccgg gccgccgggt cagcgtgaga cgcacggccg
     1261 tccgaggcgg tcctgggctt ctgaagggtg tcctgattct tgaaatccga aaacatcact
     1321 gcacctcccc gatgacggcg ctgtccgggc gcggggcgcg ttcgatgtcc atgccgccgc
     1381 cccattggga aggggtgcgc cagtagaggt tgtcgccgcc gtagccgttg tagaggtctt
     1441 cgccgccgag gtcgaggaac aggccgatcg agccgaaatc tcgacggggg ttgccgtagc
     1501 cgtgcgtgtt gtcggcgttg cggatgaagt agcggtcggc gccggcgttg tcgatgagga
     1561 tgccgacgcc gttggcggaa ccggccgcct gcgagaggtc ccaggcggtg tagagatcgt
     1621 cgccggcgcg gtcgaggagg agtccgcagg agtagtcgtg gccgacgccc tgcatgaggc
     1681 ccttgccgat gtaggcgtcg ttcccggcgt cgtcgatgag cacgccgagg gtcatgtggg
     1741 tagcgcaacc ctgggcatac tggaacgagt tgtaggtgtc gttgccggag gaatcgtaga
     1801 tgacgccgag cgaccaccag tagctcgtgc cctggccgaa gatgtcggtg tagtaggcgt
     1861 cgctgccgcg gtcgtcgatg atggcgccga tgccgcccga ggtccacggg cggaggccgt
     1921 agccgaagcc ctgcgagagc gagaggtagt ggtcctcata gcggaggatg tccttgtact
     1981 tgccgccggc atagtagctg tcggaaccgt cggtgtcgta gatcacgccc atgccctcgg
     2041 tgaagccgaa gccctgggcg tagagagcgg cgttatagca gtcgcggccc ccctcgtcga
     2101 tgagcaggcc gagtccgaac gagccggcgg ccatgacgtg cgtgtcgccg tcgtaacgat
     2161 cgtcgccggc cgcgtcgtag aggaggccga agccgaaata gccggagccg agggcgaacg
     2221 actgggcgtc gtagcggtcg ttgccgccga agtcgaggag caggccggcg gagaggcagc
     2281 cggaggcgag cgcgaaatcg gtgaggccgc ggtagcggtc atcgccggcg agatcgatga
     2341 tgatgacgcc gtgggggcgg gccgggtcgt aggagaggcg gtagacgtcg tcgccgccga
     2401 aatcgaagat gaaccagtag tccccctcgt agtagtcggg gccggggccg ccgactttcc
     2461 aaccgggttg gcggccgagg taggattcct cgcccacccc tttctcgggg atctggccga
     2521 gacctttcat cacggcgccg gcgtccaggg tcccggcggc gaggtcgcgg cgaagggcgg
     2581 cgatttcggg catcaggtcg cggaggcagt caaaaccggc ggtgaggatc gggtccttgt
     2641 cgatccgcca gccgaaagcg gtgaactcct ccgtgtactt ctcctcgatc ttctcgaccg
     2701 aatcaagctg ctcgggggtg aagaactcct cctccgcgtg actgaccagc agttccttga
     2761 attcggcggt gagaaaccgc cgctgggcgg ggctgagcga tgccagggcc gtctcggcgg
     2821 accgggggaa gatgacgtcg aggtagagac cggcccgggc catgagctgg ttcaggccgg
     2881 ggctggcgta gaacaggtta tagccctgct gcatttccgg tccggtcg
//
LOCUS       DAOOKS010000197         2913 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_41668_length_2913_cov_2.785514, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000197 DAOOKS010000000
VERSION     DAOOKS010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41668_length_2913_cov_2.785514"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..2033)
                     /locus_tag="PK112_04115"
     CDS             complement(90..2033)
                     /locus_tag="PK112_04115"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HPC11268.1"
                     /translation="MRRAMLVTAAVWLALCGSAAGAGPEYGEIAEAEWQIGPPADYPE
                     ANAVVIFDRVYRSMTIPQGEFSPQLELRRHVRMKAFNKAGAEEIGNGSFGHSEKDHVS
                     DLCAHTVTPDGQVFKVNDKDFLTRKAGSTTIVTFAFPQADSGCILEFTYKVWGGSYIR
                     WETIPFSHEVYTLETEYVLEVPVGPQYQYRAHNLPAGTGNPVVEKLPGTDMKAKKRFT
                     WRVQNVLPVQDEPYMGFERDYVPAVSLVLSDLSRKATVADAVEQWSTIGQLWETAVVD
                     EYCSEPRDFKKRLREIVKGLKTDYDKSKALYSFAVSTIETRQDGFDDGFHHDNLAGLW
                     REKFGTAGEKNLLLLAMLRSERIRAWPIMICTRDVAAFEPTWVDPLQFSHMIIFAEVE
                     QGGIYLDATTKYNAYGTLPPVCRTDIGLLLDGDKSELVKVVTNDPPSSRIDLIVYWMD
                     AEGNAACSTSALFTGYLCSNYGRRYETREANDFLDRDILNVDAPGYRRGSFEATLDSL
                     GRLRVMTGYAVEGAGRRLDDHLAVNCPATMFSRNPFESERRVFPVNFNHAFTYQAIAR
                     IVGPEGSTVSQLPPDTSFAYEGLLYERQSSSEGQAAQVHSKFTVSRPLFEVDEYMMVR
                     LFFQRVESALGEQVVFTTAKEGL"
     gene            complement(2277..>2913)
                     /locus_tag="PK112_04120"
     CDS             complement(2277..>2913)
                     /locus_tag="PK112_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018353087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPC11269.1"
                     /translation="ADVPLYVLTSGGTFSAAEEFTYNLKNLKRATVVGETTGGGAHPV
                     ATHGFPNLEVGVRVPFGRAINPISGTNWEGTGIAPDIACAADRALEVALLEAYRKLKA
                     ECGDEQLLATYDWQIDGLEAQRNPVTLEAAALQEYVGDFGPRRIALENGALVYQREGN
                     PKFRMTPMGGDLFMFADIPYFRLKFVRDAGGGIVAVEGHYEGGQVDRNERT"
BASE COUNT          499 a         1079 c          801 g          534 t
ORIGIN      
        1 cccgccccgg tcgtcggcct ccgccggcgc cgccgcggcg tcgctccatc ccagcagccc
       61 cgccgccaga aatccccaca cccgccggtt cacaagccct ccttggccgt cgtgaacacc
      121 acctgttcgc ccagcgcgga ctccactctc tggaaaaaca gccgcaccat catgtactcg
      181 tcgacctcga agagcgggcg gctcaccgtg aacttgctgt gcacctgcgc cgcctgccct
      241 tccgacgacg actgccgttc gtagaggaga ccttcgtagg cgaaggacgt atccgggggg
      301 agctggctga ccgtggaacc ctccggcccg acaatgcgcg cgatcgcctg gtaggtgaag
      361 gcgtggttga aattgaccgg gaagacgcgc cgttcgctct cgaacggatt gcgggaaaac
      421 atggtcgccg ggcagttcac cgcgaggtgg tcgtccaacc gccgccccgc cccctcgacc
      481 gcgtagccgg tcatgacgcg cagccgcccc agcgaatcca gcgtcgcctc aaaactcccc
      541 cgccggtagc cgggcgcatc gacattgaga atgtcgcggt ccaaaaagtc gttcgcttcc
      601 cgcgtctcgt agcggcgccc gtagttgctg cacagatagc ccgtgaaaag cgccgaggtg
      661 gagcaggcgg cgttcccctc ggcgtccatc cagtagacga tcaggtcgat gcggctcgac
      721 ggcgggtcgt tcgtcaccac tttcaccagt tccgatttgt ccccgtccag cagcaggccg
      781 atgtccgtcc gacaaaccgg cggcagcgtg ccgtaggcat tgtacttcgt ggtggcgtcg
      841 aggtaaattc ccccctgctc gacctcggcg aaaatgatca tgtgcgagaa ctgaagggga
      901 tcgacccaag tgggctcgaa cgccgcgacg tcccgcgtgc agatcatgat cggccaggcc
      961 cggatccgct ccgaccggag catcgccagc agcagcaggt ttttctcgcc cgcggtgccg
     1021 aacttctccc gccacaggcc ggccaggttg tcatggtgga aaccgtcgtc gaacccgtcc
     1081 tgccgcgtct cgatcgtgct cacggcaaag ctgtacagag ctttcgattt gtcatagtcc
     1141 gtcttcagcc ccttcacgat ctcccgcaac ctcttcttga aatcccgcgg ctcgctgcaa
     1201 tactcatcga caaccgccgt ttcccacaac tgcccgatcg tgctccactg ctcgacagcg
     1261 tcggctaccg tcgccttccg cgacaggtcg gagagaacca ggctcaccgc gggcacatag
     1321 tcccgctcga accccatgta gggctcgtct tgcaccggca gaacattctg cacccgccac
     1381 gtgaaccgct tcttggcctt catgtcggtg ccgggaagtt tctcgaccac gggattccct
     1441 gttcccgcag gcaggttgtg cgcccggtat tgatactgcg gccccaccgg gacctcaagg
     1501 acatattccg tctccagcgt gtacacctca tggctgaacg gaatcgtttc ccagcggatg
     1561 tagcttcccc cccacacctt gtaggtgaac tcgaggatac agccgctgtc cgcctgcgga
     1621 aatgcaaacg tgacgattgt cgtcgagccc gccttcctcg tcaggaaatc cttgtcgttg
     1681 accttgaaca cctgcccgtc gggagtcacc gtgtgcgcac agaggtcgct gacatggtcc
     1741 ttctccgagt gcccgaacga cccattaccg atctcttccg ccccggcctt gttgaacgct
     1801 ttcatgcgca cgtgccgccg cagctcgagc tggggggaga actcgccctg cgggatcgtc
     1861 atcgaccggt acacccggtc gaagatcacg accgcgttcg cctcgggata atccgccggg
     1921 ggaccgatct gccactcggc ttcggcaatc tccccgtact ccggtcccgc gccggccgca
     1981 ctcccgcaca gcgccagcca gaccgccgcc gtcaccagca tcgctctcct catggaaacc
     2041 tccgtcatga gtccttcgcc tgcgtcctac ccttgttcgc tctggagatc cgtctcggcc
     2101 gtacagccct gcgccggcgc gatccgcatc ccgcactccg gtcgtcacgc ccaactcacg
     2161 cccatagcct aggcaacgga gagtccggag tcaaggatca ttttacggcc gcgccggcgg
     2221 caaaaaaaag gccgccggga aatcccggcg gcctgccaat ctgcttgccg cccccctcac
     2281 gtccgttcgt tccggtccac ctgcccccct tcgtaatgcc cttcgaccgc cacgatcccc
     2341 ccgcctgcat cccgcacaaa cttcagccgg aaatacggga tgtccgcaaa catgaacagg
     2401 tccccgccca ttggcgtcat ccggaatttc ggatttccct cgcgctggta caccagcgcg
     2461 ccgttctcca gcgcgatgcg ccggggtccg aaatcgccga catattcctg cagcgccgcg
     2521 gcctcgagcg tcaccgggtt ccgctgcgcc tccagcccgt cgatttgcca atcgtaggtc
     2581 gcgagcaact gctcgtcccc gcactccgct ttcagcttcc ggtaggcctc cagaagcgcg
     2641 acctcgagcg cccggtcggc tgcgcacgcg atgtccggtg cgatccccgt cccttcccaa
     2701 ttcgtccccg aaatcgggtt gatcgcccgc ccgaacggca ccctcacgcc cacctcgagg
     2761 ttcggaaatc cgtgcgtcgc caccgggtgc gccccaccgc ccgtggtctc cccgacaacc
     2821 gtcgcccgct tcagattctt cagattgtac gtaaactctt ccgccgccga aaaggtgccc
     2881 cccgaggtga gcacgtacag ggggacgtcg gcc
//
LOCUS       DAOOKS010000198         2909 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_41746_length_2909_cov_3.304835, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000198 DAOOKS010000000
VERSION     DAOOKS010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41746_length_2909_cov_3.304835"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..2786)
                     /gene="polA"
                     /locus_tag="PK112_04125"
     CDS             complement(60..2786)
                     /gene="polA"
                     /locus_tag="PK112_04125"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HPC11270.1"
                     /translation="MTPDRKTVYLVDGSAIFYRAYFAFLRNPLINSKGENTSAVFGFV
                     NSLLKVIRDEKPDYLAVAWDSKAPTVRHERYEEYKSTRAKMPEDLVAQLPAIREAVEA
                     FRIRHFEMEGFEADDIIGTLASRCAKAGFDVWCVTGDKDFFQLVTDRIRIYTPQPGAD
                     QAVKMGREEVKAKFGVYPEAVIDKLALMGDSSDNVPGIPGVGPKTADALLEQFGSLEA
                     VLAGANRIKAKAVRARVEAHADLARLSKELVTICTDVPIACDLEQLRFREPDHAKLVE
                     LFQRLEFNRLLKQLVPEAAAAAGVTDEAPGATKPGYHVVAALKDLKTLLKKLSTCRKV
                     AVDTETTSLNPLAAELVGISLSARANEAYYLPLGHTGEGAVNLPLPEALAEVKKLLEN
                     PDVQKFGQNIKYDLAVLRKYGIEIEPVRFDTMLASYVIDPSSRQHSLDALALQHFNHR
                     KTPITDLIGAGKKQISFAQVPVDKACDYSCEDADYTYRLRGKLAPVIDQLGMNSLYYD
                     IEVPLIKVLADMEREGVRIDVSFLGELSVQMERRLEEIRREIYRIAGTEFNINSTQQL
                     AHILFEKLGLPTKGKTTKKTGFSTDVRVLEELAALHELPARILDFRQLTKLKSTYVDA
                     LPKLVDGRTGRVHTSFNQTIAATGRLSSTDPNLQNIPVRTDEGREIRKAFVPRDEGHT
                     LLVADYSQIELRILAHMSEDSTLIEAFRRGEDIHARTAAEVYGVEIGQVTPEQRRAAK
                     TANFAVIYGVTAYGLAAQSALDVGQAKAFIATYFERYPGIRRYLEATKEFARARGYVT
                     TMFNRRRTIREINDRNVNVRQFAERAAINTPIQGTAADMIKKAMIRIHGRLRATRSRM
                     ILQVHDELVLDVPREELDEVREIVRDGMEQAVSLKVPIVVSLGSGPNWLDAK"
BASE COUNT          484 a          839 c         1037 g          549 t
ORIGIN      
        1 accgatcaac ataccggtaa taaaggctta ttcgggaaaa gggcaagccc ccggcggcgt
       61 catttggcgt ccagccagtt gggaccggac ccgagcgaca ccacgatcgg gaccttgagc
      121 gacaccgcct gctccatgcc gtcgcggaca atctcccgca cctcgtcgag ctcctcccgg
      181 ggcacatcca ggacgagctc gtcgtgcacc tggaggatca tgcgggagcg tgtggcgcgg
      241 agccgcccgt ggatccggat catggctttc ttgatcatgt cggcggcggt accctgaatg
      301 ggcgtgttga tcgccgcgcg ctcggcaaac tggcggacgt taacgttgcg gtcgttgatt
      361 tcgcggatgg tgcgccgccg gttgaacatg gtcgtcacgt agccgcgcgc acgggcgaat
      421 tccttggtgg cctcgaggta gcgccggatg ccggggtagc gctcgaagta ggtggcgatg
      481 aaggccttgg cctgcccgac atcgagggcg gactgggccg cgaggccgta ggcggtgacg
      541 ccgtagatga cggcgaagtt ggccgtcttg gcggcccgcc gctgctcggg ggtcacttgg
      601 ccgatctcca caccgtagac ctccgcggcc gtgcgggcgt ggatgtcttc gccgcgtcga
      661 aaggcctcaa tgagggtgga gtcctccgac atgtgggcga gaatgcgcag ttcaatctgg
      721 gagtaatcgg ccacgagcag ggtgtggccc tcgtcgcgcg ggacaaaggc tttgcggatc
      781 tcgcgccctt cgtcggtgcg gacggggata ttctggagat tcgggtcggt agaggagagg
      841 cggccggtcg cggcgatcgt ctggttgaag gacgtgtgca cccgccccgt acgcccgtcg
      901 accagcttcg ggagagcgtc aacgtaggtg ctcttgagtt tggtcagttg gcggaaatca
      961 aggatgcggg cggggagttc atggagggcg gccagttcct cgagcacccg gacgtcggtc
     1021 gaaaagcccg tcttcttggt ggtcttcccc ttcgtcggga ggccgagttt ctcaaagagg
     1081 atgtgcgcca actgctgggt ggagttgatg ttgaattcgg taccggcgat gcggtagatc
     1141 tcgcggcgga tttcctccag ccggcgctcc atctgcaccg acaactcgcc gagaaatgag
     1201 acgtcgatgc gcactccctc ccgctccatg tcggcgagca ccttgatgag cggcacctcg
     1261 atgtcgtagt agaggctgtt catgccgagc tggtcgatca ccggcgcgag tttgccgcgg
     1321 aggcggtagg tgtagtcggc gtcctcgcac gaatagtcgc acgctttgtc gaccggaacc
     1381 tgggcgaacg atatctgctt cttccccgca ccgatgaggt cggtgatggg cgtcttgcgg
     1441 tggttgaaat gctggagcgc gagggcatcg agtgagtgct gccgcgacga tgggtcgatg
     1501 acgtacgaag ccagcatggt gtcgaagcgg accggctcga tttcgatgcc gtacttgcgc
     1561 aggactgcca gatcgtactt gatgttctgg ccgaacttct gcacgtccgg gttttcgagg
     1621 agtttcttca cctcggcgag ggcctccgga agcggcaggt tcaccgcccc ctcccccgtg
     1681 tggccaagcg gcaggtagta ggcctcgttg gcgcgggccg acagggagat gcccacgagc
     1741 tcggcggcga gcgggttcag cgaggtggtc tcggtgtcaa cggcgacttt ccggcaggtc
     1801 gagagcttct tcagaagagt cttgagatcc ttgagggcgg cgacgacatg gtagcccggc
     1861 ttggtggcgc cgggcgcctc gtcggtgacg cccgcggcgg ccgccgcctc ggggacaagt
     1921 tgtttcagaa gacggttgaa ctcgagacgc tggaacaatt cgacgagttt ggcgtgatcg
     1981 ggttcgcgga agcggagctg ctccaggtca caggcgatcg ggacatcagt gcagatggtc
     2041 accagctcct tggagagacg ggcgaggtcg gcgtgggcct cgacccgggc ccggacggct
     2101 ttggccttga tccggttggc gccggcgagg accgcctcga ggctgccgaa ctgctccagg
     2161 agcgcatcgg cggtcttggg accgacgccg gggatgccgg gaacattgtc ggaactgtcg
     2221 cccatgaggg cgagcttgtc gatgaccgct tccggataga cgccgaattt cgcctttact
     2281 tcctcgcggc ccatcttgac ggcctgatcg gcgcccggct ggggcgtgta gatgcggatg
     2341 cggtcggtaa cgagctggaa gaaatccttg tcgccggtga cgcaccagac gtcgaagccc
     2401 gccttcgcgc agcggctggc gagggtcccg atgatgtcgt cggcctcgaa accctccatc
     2461 tcaaagtgcc ggatgcgaaa cgcctcgacc gcctcgcgga tcgccgggag ctgggccacg
     2521 aggtcctcgg gcatcttggc gcgagtggat ttgtactcct cgtagcgctc gtggcggaca
     2581 gtcggggcct tggagtccca ggcaacggcg aggtagtcgg gcttctcatc gcgaatgacc
     2641 ttcagcagcg aattgacaaa gccgaagacg gccgaggtgt tctccccttt cgagttgatc
     2701 agcgggttgc ggaggaaagc gaagtaggcg cggtagaaga tggccgagcc atcgaccagg
     2761 tagacagtct tgcggtcggg cgtcatcggt acaacccctc tttttctatg tagtcgagca
     2821 cgggcgcggg aagccaggcc agcagccggg ggcgcggcgc gccgcggccg atcagggcgc
     2881 gcaggtcggt cgacgaaacc gcgggcgtt
//
LOCUS       DAOOKS010000199         2908 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_41769_length_2908_cov_2.773572, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000199 DAOOKS010000000
VERSION     DAOOKS010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41769_length_2908_cov_2.773572"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="PK112_04130"
     CDS             complement(<1..479)
                     /locus_tag="PK112_04130"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HPC11271.1"
                     /translation="MNENPEQIGRYLDYLFTFGPGLVYVTLFAACFMENVFPPFPGDS
                     FIAAAGALVGAGRLDLYLSFALVLAGGMSSVMLMYGVGRRYGREYFLVKDYKYFSAAD
                     ILAFERHLARWGGLLLVCSRFVIGLRSAIALGAGIARYPVRRMAVYSLLSYIVFTAAL
                     "
     gene            complement(472..960)
                     /gene="ispF"
                     /locus_tag="PK112_04135"
     CDS             complement(472..960)
                     /gene="ispF"
                     /locus_tag="PK112_04135"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HPC11272.1"
                     /translation="MADLRIGHGFDVHAFADDRELVVGGVIIPHERGLAGWSDADVLL
                     HAIMDALLGAAGLPDIGQQFPPGDPAYRQADSAVLLSRVGHLVRSAGYTRILNIDAII
                     MAERPRMNPHIPAMRKAIAEVLRIKEAQVNVKATTTEQLGFVGREEGIAASAVCLIAG
                     DE"
     gene            complement(953..1657)
                     /gene="ispD"
                     /locus_tag="PK112_04140"
     CDS             complement(953..1657)
                     /gene="ispD"
                     /locus_tag="PK112_04140"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HPC11273.1"
                     /translation="MRDRMITAAVIVAGGQSLRFGGEVPKQFREVAGRPLLAWTISRF
                     EAAGSIDRIVVVAAEDYLGYAGEQVVDRYGFAKVQQIVSGGTERRESVWRGLEALPIA
                     TGFVAIHDGARPLVRPADIDRVVGAAKRDRAAILAVPVPDTLKRVRDGYVIATVDRSY
                     LYGAQTPQVFQYDLIMAAHREAAGAAGRYTDDASMVESRGFKVTVVEPSGPNIKITTP
                     EDLVIAEALLAREGHG"
     gene            complement(1742..2779)
                     /gene="queA"
                     /locus_tag="PK112_04145"
     CDS             complement(1742..2779)
                     /gene="queA"
                     /locus_tag="PK112_04145"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HPC11274.1"
                     /translation="MDISQFDYHLPPELVAQFPSRRRDESRLMIVDRAGDGCTIVPFR
                     RIADYLEDGEALVVNDTRVFKARLRGNRSSGARVEVFLVRPLDRGDGDAWLALVSPSR
                     RLREGEDVLFGDDRIRLREYQGKGRWAVGFASRAARERIIRRHGRVPLPHYIRREDGP
                     ADLRRYQTVFADRARTGAVAAPTAGFHFTRPLLHDLAGRGVRLVRLTLHVGPGTFKPV
                     SADNIDDHVVDPEWAELPESAARVLNGVRDGGGRITAVGTTSVRTLESVVRGDGRFVP
                     LAGMVDLYIRPGYHFRGVDRLITNFHLPRSSLLILVAAFAGRDRILEAYRYAIAQRMR
                     FYSYGDAMLIL"
BASE COUNT          508 a         1057 c          925 g          418 t
ORIGIN      
        1 agggccgccg tgaacacgat gtaggacagc agcgagtaga ccgccatgcg ccggaccggg
       61 tagcgggcga tgcccgcccc cagcgcgata gcgctccgca ggccgatcac gaaccgcgag
      121 cacaccagaa ggagcccccc ccagcgcgcc aggtgccttt cgaaagccag gatgtccgcc
      181 gccgagaaat acttgtagtc tttgaccagg aaatactcgc ggccgtagcg gcggcccaca
      241 ccgtacatga gcatgaccga actcatcccc cccgcgagca ctagcgcgaa actcaggtac
      301 aaatccagcc gccccgcccc gaccagcgcc ccggccgccg caatgaaact gtcgccggga
      361 aaaggcggga acacgttctc catgaagcag gccgcgaaca gcgtcacgta caccagcccg
      421 gggccgaacg tgaacaggta gtccaggtag cggccgatct gttcggggtt ctcattcatc
      481 gccggctatc agacacaccg cgctcgcggc gatcccctct tcccgcccga cgaaaccgag
      541 ttgctcggtg gtcgtggctt tcacattcac ctgcgcctcc ttgatccgca gcacctccgc
      601 gattgccttg cgcatcgccg gaatatgcgg gttcatcctg gggcgctcgg ccatgatgat
      661 cgcgtcaatg ttgaggatgc gggtataccc ggccgaccgc accaggtggc cgacccgcga
      721 caggagcacg gccgagtcgg cctggcggta ggccggatcg cccggcggga actgctgccc
      781 gatatcgggc aggccggccg cccccaggag cgcatccatg atggcgtgca ggagaacgtc
      841 ggcgtccgac cagccggcca gcccgcgctc gtgcgggata atcactccgc ccaccaccag
      901 ctcgcggtcg tcggcgaacg cgtggacgtc aaacccgtgc ccgatgcgca ggtcagccat
      961 gcccctccct ggccaggagg gcttcggcga tgaccagatc ctccggggtc gtgattttga
     1021 tgttgggtcc gctcggctcc accaccgtca ccttgaatcc gcggctctcc accatcgacg
     1081 catcgtcggt ataccgcccg gccgcgccgg ccgcctcccg gtgcgccgcc atgatgagat
     1141 cgtactgaaa cacctgcggc gtctgcgcgc cgtacaggta actgcggtcc acggttgcga
     1201 tcacgtagcc gtcgcgcaca cgcttgagcg tgtcgggcac cgggaccgcg aggatggcgg
     1261 cccggtcccg cttcgccgcg ccgacgacgc ggtcgatgtc ggctggccgg accagcggcc
     1321 gggcgccgtc atggatggcc acgaaaccgg tggcgatcgg cagcgcttcc agccctctcc
     1381 acaccgactc ccggcgttcg gtcccgccgg agacgatctg ctgcaccttc gcgaacccgt
     1441 accggtccac aacctgttct ccggcgtagc cgagatagtc ttcggccgcc acgaccacaa
     1501 tccggtcgat ggagccggcc gcttcgaagc ggctgatggt ccaggccaac agcggccgcc
     1561 cggcgacttc ccgaaactgc ttcgggactt cgccgccgaa gcggagcgac tgcccgccgg
     1621 cgactatgac ggctgcggtg atcatgcggt cgcgcatctt tccggtttcg gcgggacgcc
     1681 ggccatggcg tccggccacg ggcgggaata tacgcgccgc ccggtcccga caaaagcgaa
     1741 ttcagaggat gagcatcgcg tcgccatagc tgtagaaccg catccgctgc gcgatcgcgt
     1801 accgataggc ctcgaggatc cgatcccgtc cggcgaacgc cgcgacgagg atgagcagcg
     1861 aggaacgcgg gaggtggaaa ttggtgatca ggcggtccac gccgcggaag tggtaccccg
     1921 gccggatgta gagatcgacc atcccggcca gcggcacgaa ccgcccgtcc ccccgcacga
     1981 ccgattcgag cgtccgcacc gaggttgtcc cgacggccgt gattcggccg cccccatccc
     2041 ggacgccgtt caacacccgg gcggcgctct ccggcagttc cgcccactcc gggtccacca
     2101 cgtggtcgtc gatgttgtcc gccgacaccg gtttgaacgt ccccggcccc acgtggaggg
     2161 tgagtcggac gagacggacc ccccggccgg ccagatcgtg caggagcggc cgggtaaagt
     2221 gaaatccggc ggtcggcgcc gccaccgccc ccgtccgcgc gcggtcggcg aacacggtct
     2281 ggtaacgccg gagatcggcc gggccgtcct cccggcgaat gtaatgcggc agtggcacgc
     2341 ggccgtgccg gcggatgatc ctctcccgcg ccgcccggga ggcaaatccc accgcccacc
     2401 ggcctttgcc ctggtattcc cgcagcctta tccgatcgtc gccgaacagc acgtcctccc
     2461 cctcgcggag cctccgcgat gggctgacta gcgccagcca ggcgtctcca tcccccctgt
     2521 caagcggacg caccagaaac acctcgaccc gcgcgccgct ggagcggttg ccccggagcc
     2581 gcgccttgaa tacccgggta tcgttcacca ccagcgcctc gccgtcctcc aggtagtcgg
     2641 cgatccggcg gaacggcaca atcgtgcagc cgtcgccggc ccggtcgaca atcatgagcc
     2701 gggattcgtc ccgccggcgc gagggaaact gcgcgaccaa ctccggcgga aggtggtagt
     2761 cgaactggct gatatccata cggcgtccgg ccgccgggcg caaccgatcc cggccgccgc
     2821 cacccatgat acgcaaccgc ggctgcctgc gaaacaaatt gataagggcg gtgaacagcg
     2881 gtgaaagacg gcggcgatag aaacgagc
//
LOCUS       DAOOKS010000200         2900 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_41908_length_2900_cov_3.447452, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000200 DAOOKS010000000
VERSION     DAOOKS010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41908_length_2900_cov_3.447452"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1542
                     /locus_tag="PK112_04150"
     CDS             <1..1542
                     /locus_tag="PK112_04150"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HPC11275.1"
                     /translation="RLELLPALIAYQSGGRDEDDLTTVFESGDAEAEGELNVRYRLTP
                     GLTAEATVNPDFSQVESDAAEIDVNTTYALSYPERRPFFQEGSDLYGTAIPAIYTRAV
                     NDPSIALKLTGRQGRTSFFFLAAVDDTSLQLIPLEEETEYAARGKSYAAIGRVRRTFA
                     DDSYLGALFTDRRLDAGDGSGTVFGIDGALRVREKYLLSTQMLGSYHRESGAPGDAGA
                     GGEPGPAFDDEGHTVSPDGEAYSGMASYTQFQRYSRRWELEISHTATSPTFRADNGFI
                     QRAGIQEADVEGSLSFRPNGKLVTTVTTALHVGRLWAWDGGRKDEWIMPEIAFELPCQ
                     TELYGSLVLSREQFRGVWFKDIRRVEGEVSTRVLHSFRAGAAVTYGNSIARMDNPVMG
                     RELSAGFEAIIKPLQQFTIEPTWEYYRIRDRETGERLEEGYITRTRFNYQFTRELFAR
                     LVVQYDDFDDRLNFEPLLTYKLNPFTIFYIGAAFRHGWDDERGIDGRLIQRQFFVKFQ
                     YLLRA"
     gene            1600..2409
                     /locus_tag="PK112_04155"
     CDS             1600..2409
                     /locus_tag="PK112_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015121750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HPC11276.1"
                     /translation="MPQHIVQVDSFTSTPFRGNPAAVCVMAGPREPEWMQAVAAEMNL
                     SETAFVHPLDGGYALRWFTPTTEVPLCGHATLAAAHVLWSEHHLNPDRQIRFHTKSGL
                     LVVTRQGELISMDFPALPVEACAPPPGLREALGLEARFVGRDKFHFLIETADEQAVRA
                     CAPDFTALARAAREGVTVTAAAGGEYDFVSRFFAPALGINEDPVTGTAHCALGPYWAG
                     KLGKPTLAGYQASRRGGAVQVTVRGERVLLAGRAVTVLRGELAAAESPRHG"
     gene            2538..>2900
                     /locus_tag="PK112_04160"
     CDS             2538..>2900
                     /locus_tag="PK112_04160"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005991287.1"
                     /note="catalyzes the formation of glycerone phosphate and
                     D-glyceraldehyde 3-phosphate from D-fructose
                     1,6-bisphosphate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HPC11277.1"
                     /translation="MIGKIEQLLGDQKNLLKHECKTIPKSKLHLPGPDFIDRVLVSSD
                     RSPATLRNLAAMFNHGRLAGTGYLSILPVDQGIEHSGAASFAPNPDFFDPENIVKLAV
                     EGGCNAVASTLGVLGAVSR"
BASE COUNT          475 a          908 c         1045 g          472 t
ORIGIN      
        1 cgcctcgaac ttttgccggc gctgatcgcc taccagagcg gcggccgcga cgaggacgac
       61 ctcacgacgg tgtttgagag tggcgacgcc gaggcggagg gggagctcaa cgtgcgctac
      121 cgcctgactc ccgggctgac ggccgaggcg acggtgaacc cggatttcag ccaggtggag
      181 tcggatgcgg ccgagatcga cgtcaacacc acctacgccc tgtcctatcc ggagcggcgg
      241 ccgttctttc aagaggggag cgatctgtac ggcacggcga tcccggcgat ctacacgcgc
      301 gcggtcaacg acccgagcat cgcgctgaaa ctgaccgggc ggcaggggcg aacaagtttc
      361 ttcttcctcg cggcggtcga cgacacctcg ctgcagctta ttccgctcga ggaggagacc
      421 gagtatgcgg cgcgcggcaa atcgtacgcc gccatcggcc gggtgcggcg cacctttgcc
      481 gatgactcct acctcggtgc gcttttcacc gaccggcggc tcgacgccgg ggacggttcg
      541 gggacggtgt tcgggatcga cggcgccctg cgcgtccgtg aaaaatacct cctctcgacc
      601 cagatgctgg gaagctacca tcgggaatcg ggggcgccgg gagacgcggg ggcgggcggg
      661 gaaccggggc cggcgtttga cgacgagggg cacacggtgt cgccggacgg ggaagcctac
      721 agcggcatgg cctcctacac ccagttccag cgctactccc ggcggtggga gttggagatc
      781 agccacacgg cgaccagccc gactttccgg gcggacaacg ggtttatcca gcgcgccggc
      841 atccaggagg cggatgtcga gggcagtctg tcgtttcgcc ccaacgggaa actggtgacg
      901 acagtcacca cggccctcca cgtgggccgc ctgtgggcgt gggacggcgg gcggaaggat
      961 gagtggatca tgccggagat tgccttcgag ttgccctgcc agaccgagct gtacggatcg
     1021 ctggtgctca gccgcgagca gttccgggga gtgtggttca aggacatccg gcgggtggag
     1081 ggggaagtga gcacgcgcgt gctgcacagt ttccgcgccg gcgcggcggt gacgtacggc
     1141 aattcgatcg cccggatgga caacccggtg atggggcggg agctgtcggc cggattcgag
     1201 gcgatcatca agccgctgca gcagttcacg atcgaaccga cctgggaata ctaccggatc
     1261 cgggaccgcg agacgggcga gcgcctcgag gaggggtata tcacgcggac gcgcttcaat
     1321 taccagttca cgcgcgagtt gttcgcgcgg ctggtcgtgc agtacgacga ttttgacgac
     1381 cggctgaatt tcgaaccgct cctgacctac aagctcaacc ccttcaccat cttctacatc
     1441 ggggcggcgt tccgccacgg gtgggacgac gagcggggga tcgacgggcg cctcatccag
     1501 cggcagttct tcgtcaagtt ccagtacctc ctccgggcgt agcggccgcg ggggagttcg
     1561 cccttgaccg cggcggccaa cgggctatac cttgcctgta tgccccagca catcgtacag
     1621 gtggacagct tcacgagcac gccgtttcgc ggcaacccgg ccgcggtgtg cgtgatggcg
     1681 ggcccgcggg agccggagtg gatgcaggcg gtggcggccg agatgaacct ctcggagacg
     1741 gccttcgtgc acccgctcga cggcgggtat gcgctgcgct ggttcacgcc cacgaccgag
     1801 gtgccgctgt gcggccatgc gacgctggcg gccgcccacg tgctgtggag cgagcaccac
     1861 ctcaatcccg accggcagat ccggtttcac accaagagcg gcttgctcgt ggtcacccgg
     1921 cagggggagc tgatcagcat ggatttcccg gcgctgccgg tggaggcgtg cgcgccgccg
     1981 ccggggctgc gcgaggccct ggggctcgag gcgcgctttg tcggccgcga caagttccat
     2041 ttcctgattg agacggccga tgagcaagcg gtgcgcgcct gtgcgcccga cttcaccgcc
     2101 ctggcgcggg cagcgcggga aggggtgacg gtgaccgccg cggccggcgg ggagtacgac
     2161 ttcgtctcgc ggtttttcgc gccggccctg gggatcaacg aggatccggt gaccgggacg
     2221 gcgcactgcg cactcggtcc ctactgggcg gggaaactgg gcaaaccgac gctggcgggt
     2281 taccaggcct cgcggcgtgg gggggcggtg caggtgacgg tgcgcggcga gcgcgtgctc
     2341 ctggcggggc gggcggtgac cgtgctgcgc ggggaactgg cggccgccga atcgccgcgg
     2401 cacgggtgac cgaccgtcgc cggcgcggcc gccgagcttt taacttggag aaacagcggg
     2461 tgtgacagta gttttgagcg gtacgtacag attcgtcaac gccgtacccg cggctgttaa
     2521 cataggagag tggtatcatg ataggcaaaa tcgagcagct tctgggcgac cagaagaatc
     2581 tgttgaagca cgagtgcaag acgatcccga agtcgaaact gcacctgccc ggccctgatt
     2641 ttatcgatcg ggtcctggtt tcgtcggacc gcagtccggc caccctgcgc aatctcgcgg
     2701 ccatgttcaa ccacgggcgg ctggccggca ccgggtacct ctcgatcctt ccggtcgacc
     2761 aggggatcga acactccggc gcggcctcgt tcgcccccaa cccggatttc ttcgaccccg
     2821 agaatattgt gaagctggcg gtggaggggg ggtgcaacgc ggtggcctcc acgctcggcg
     2881 tcctcggagc ggtgtcgcgc
//
LOCUS       DAOOKS010000201         2895 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_42021_length_2895_cov_3.619014, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000201 DAOOKS010000000
VERSION     DAOOKS010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42021_length_2895_cov_3.619014"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1799)
                     /gene="rpsA"
                     /locus_tag="PK112_04165"
     CDS             complement(<1..1799)
                     /gene="rpsA"
                     /locus_tag="PK112_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HPC11278.1"
                     /translation="MATAKQSETATQRLSKRKAQSTRKTKRVKTKAARPEGIDLEQQG
                     LSAEDQQQRERVVTSRHKRVAEKAQQRLSRTEEGLDDGSSAVQAVRITDIAGVVYDPD
                     EYQQMVDMYDQTIKDIKEGEVVAGRVIGINRDDVIVDVGFKSEGIIAMGEFQQPVTVR
                     MGDEIEVYLEQIEDAHGQLILSKQKADFMRVWEKIREVHDSGEKISGIVARRIKGGLV
                     VDIMGVDAFLPGSQVALRQVPDFDALIGQSMDVKIIKINKARRNIVVSRRIVLEEERE
                     KMRAHLLNEIAVGQVRQGIVKNITDFGVFIDLGGVDGLLHITDMSWGRIRHPSELVSL
                     GDRIDVKILDFDERTSRISLGLKQMTPYPWENIEQKYPLGRKVKGRVVSITDYGAFVE
                     LEKGIEGLIHISEMSWTQHIKHPSKIMNVNDEVEAMVLSVDKENEKISLGIKQMEPDP
                     WTTIEMKYPPGRIVTGKVRNLTAFGAFVELEEGIDGLVHISDMSWTKRIQHPSEVMKK
                     GDRVDVKILKIDHENRRISLGYKQLEDDPWPEIARKYGVGAEVLGTIQRVLDRGVVVE
                     LDGAVEGFVPATQLGRKDLTDPTGVFKEGDEIPL"
     gene            complement(2090..2704)
                     /locus_tag="PK112_04170"
     CDS             complement(2090..2704)
                     /locus_tag="PK112_04170"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPC11279.1"
                     /translation="MKILYWTGYFIAKIAGRVIFRGRIIGKEHVPRSGGFILASNHRS
                     YFDPPLAGSATGREMAFFAKAELFAVPVLGSLIRRTNAMPVKRGSVDRDALKTAVATI
                     RKGYGLTVFPEGTRSRDDSFLEPKPGLGLIALHAECPIVPCYLHGTNKLKQCLLGREK
                     LRVAFGEPLPVEWVRSFPKSKEGYVQLAREVMDRIAKIKAASGL"
BASE COUNT          477 a          917 c          889 g          612 t
ORIGIN      
        1 agggggattt cgtccccctc cttgaagacg ccggtgggat cggtgaggtc tttgcgtccg
       61 agctgggtag ccggcacgaa cccctcgacc gccccgtcga gctccacgac cacgccgcgg
      121 tcgagaacgc gctggatggt gccgaggacc tcggcgccga cgccgtactt gcgcgcgatc
      181 tcgggccagg gatcatcctc gagctgcttg tagcccagcg aaatgcggcg gttctcgtgg
      241 tcgatcttga ggatcttcac atccacccga tcccccttct tcatgacctc ggaggggtgt
      301 tggatacgct tggtccacga catgtcggag atgtgcacga ggccgtcgat cccctcttcg
      361 agttcgacga aggcgccgaa agcggtgagg ttgcgcacct tgccggtgac aatgcggccg
      421 ggcgggtact tcatctcgat cgtggtccac ggatccggct ccatctgctt gatcccgagc
      481 gagatcttct cgttctcctt gtccaccgac agcaccatcg cctcgacctc gtcgttcacg
      541 ttcatgatct tggaggggtg cttgatgtgc tgggtccagg acatttcgga gatgtggata
      601 aggccctcga tgcccttttc gagttcgacg aatgcgccgt agtcggtgat cgagacgacc
      661 cgtcccttga ccttgcggcc gagcgggtat ttctgctcga tgttctccca cgggtacggc
      721 gtcatctgtt tcaggccgag cgagatgcgc gacgtccgtt catcgaagtc gagaattttc
      781 acgtcgatcc gatcgccgag cgagaccagc tcgctggggt ggcgaatccg tccccacgac
      841 atgtcggtga tgtgcaggag gccgtcgacc ccgccgaggt cgatgaacac gccgaaatcg
      901 gtgatgttct tgacgattcc ctggcggacc tggccgaccg cgatctcatt gagcaggtgc
      961 gcccgcatct tctcgcgctc ctcttcgagc acgatgcggc gggagacgac gatattgcgg
     1021 cgggccttgt tgatcttgat gatcttgaca tccatcgact gcccgatgag agcgtcgaaa
     1081 tccggcacct ggcgcagcgc cacctgtgat cccgggagga aggcatcgac gcccatgatg
     1141 tcgaccacga ggccgccctt gatccggcgc gcgacgatcc cggaaatctt ctccccgctg
     1201 tcgtggacct cgcggatctt ctcccagacc cgcatgaaat ccgccttctg cttagagagg
     1261 atcagctgtc cgtgggcgtc ctcgatctgc tccaggtaga cctcgatttc atcacccatg
     1321 cggacggtga cgggctgctg gaactcgccc atggcgatga tgccctcgga cttgaagccg
     1381 acgtcgacga tgacatcgtc gcggttgatc ccgatcacgc gcccggcgac aacctcgccc
     1441 tctttgatgt ctttgatggt ctggtcgtac atgtcgacca tctgctggta ctcgtcgggg
     1501 tcgtagacga cgccggcgat gtcggtgatg cggacggcct ggacagcgga cgacccgtcg
     1561 tcaagaccct cctcggtgcg gctgaggcgc tgctgcgcct tttccgccac ccgtttgtgg
     1621 cggctcgtga caactcgctc gcgctgctgc tggtcctccg cagacaaacc ctgctgctcg
     1681 aggtcgatgc cctcgggccg ggcggctttt gtcttgacgc gtttggtctt tcgggtggat
     1741 tgcgctttgc gcttggacag tctctgagtt gcggtttcgg attgtttggc cgttgccatg
     1801 ttgactgtac gctacctcct aatcaagggc cgacagtttg gaattttccc gagggaagac
     1861 tgccggatcg gctgcccgcc ctctcagagc gggaagcctt gctatataac acataggatc
     1921 ggaagcgcaa gcgaattctg cggtcctttt tggcacgttc ccgccggccc tcggtccgcc
     1981 gcaccgggct gattccccgc cgggagtgcg tcgtagggcg ccctcacccg gtcaacaacg
     2041 actctgtctt gtcgggccgc cgcgccgacc gccatcgctt tctgccgccc tacagcccgg
     2101 aagcggcctt gatcttcgca atcctatcca tgacctcccg cgccaactgc acatacccct
     2161 ctttggattt cgggaaggat cgtacccact cgaccggcag gggttcgccg aacgcgacgc
     2221 gcagcttctc gcgtcccagg aggcattgct taagcttatt tgtcccatga agataacatg
     2281 gaacgatcgg gcactcggcg tgcagcgcaa tcaaccccag cccgggcttg ggctccaaga
     2341 acgagtcgtc cctggaccgc gtcccctccg gaaacaccgt cagcccatat cccttgcgga
     2401 tcgtcgccac cgcagtcttg agcgcgtccc gatcgaccga tccgcgcttc accggcatgg
     2461 cgttggttct ccggatgagc gatccgagca ccggcaccgc gaagagctct gccttggcga
     2521 agaaggccat ctcccgtccg gtggccgatc ccgccagcgg cggatcgaag taggagcgat
     2581 ggttgctcgc gagaatgaag ccgccggacc gggggacgtg ctccttgccg atgatccgcc
     2641 cgcgaaagat gacgcggcct gcgatcttcg cgatgaagta gccggtccag tagagaatct
     2701 tcatagggtt gatccgtccg acggttcggg acatggtaca gcggggggcc ggggatggca
     2761 atgaaaaagg gcggcttcgc agccgtgatc ggttcgtact cagcgtcggc cagcggggcc
     2821 atggacgtta agatttgcct gcttgtgccg gcacccgcac gttatctgtt gacagggcgg
     2881 tggcgccttg cggat
//
LOCUS       DAOOKS010000202         2890 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_42117_length_2890_cov_2.841623, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000202 DAOOKS010000000
VERSION     DAOOKS010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42117_length_2890_cov_2.841623"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(129..>564)
                     /locus_tag="PK112_04175"
     CDS             complement(129..>564)
                     /locus_tag="PK112_04175"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPC11280.1"
                     /translation="DTFLKAIDRIDQFRGEAAFGTWLYAITLNETRMALAREKRADLR
                     PIEDYLPAHDRDHGGPTVYDWRDPHRLLEQGELRTAIDRAIGELPLPYREAFVLRYID
                     ELPIKEVAELLGESVAATKSRVLRTRLAVRDRLAKQFEGHNG"
     assembly_gap    565..574
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(978..2090)
                     /locus_tag="PK112_04180"
     CDS             complement(978..2090)
                     /locus_tag="PK112_04180"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HPC11281.1"
                     /translation="MPRITRPEPERDLFKNRYSRASVTAMGRAIKAVCPTFDLPGFVA
                     SVITDDFPRLEFKDRGRAIARALAEYLPGDYGRAVDVLIAVAPRLDGFHNWAAMTFIE
                     LQGLAHFEDSVRGMKALTRYSTAESAIRPFLNSFPDRLLPILHEWAADPDEHVRRLAA
                     EGSRPRGVWVAHIGAYKKDPRPVLALLEKLKADPSKYVRTAVANNLNDISKDHPDLVI
                     TAALRWLKDGDAGTTWIVRHACRSLIKKGDPRVFPIFGFTANPKISVTGPRPPRRAVA
                     IGADAAFPFTVTSRAQSRQKLAIDYRVHYVKKNGKTSPKVFKLAEKTIAPGETLVLQV
                     RQSFADVSVRKHVPGRHRLEIIVNGRTVAAADFTLA"
     gene            complement(2142..>2890)
                     /locus_tag="PK112_04185"
     CDS             complement(2142..>2890)
                     /locus_tag="PK112_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019225345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HPC11282.1"
                     /translation="FTTCSVTSTTLLSSNDMVRIGDWIEMPKYGADGEVVDVTLHVVK
                     VRNWDNTITNIPSYHLIADSFKNWRGMQESGGRRIMRAVCIDVSSIRFCTPEMLDRFE
                     QFQLITEYVRQRRGEIEEWNRAHGIDRRHPVNGRNMTNLGTFRAYVSAYLRRHPLINP
                     DLPLLVRHREPTEKGLPLQIYAFSRDKIWTNYEALQADLFDHILAAVPLFDLRIFQLP
                     GGHDVRGLAALLPQDHRPAPPAAAGGAPSP"
BASE COUNT          470 a          855 c          999 g          556 t
ORIGIN      
        1 ggagggtatc gaccatgatc cggcagtttt cgcaccggcc gatatgccgc tcgagttcct
       61 cgcagagctc cggcgagatt tcgccatcga ggtagtcgtt caggtcggtg acgtattcag
      121 cgcatttctt acccattgtg gccctcgaac tgtttcgcga ggcggtcccg gaccgccagc
      181 cgcgtccgga gcacccggga cttggtcgcg gcgaccgact cgccgagcag ttcggctacc
      241 tcttttattg ggagttcatc gatatagcgc aggacgaatg cttcgcggta cggcaggggg
      301 agctcgccga tcgcgcgatc gatcgcggtg cgcaattctc cctgctccaa aagccggtgg
      361 gggtcccgcc agtcgtacac cgtcggcccg ccgtgatccc ggtcgtgggc agggaggtaa
      421 tcttcgatcg gccggaggtc ggcgcgtttt tcgcgggcca gcgccatgcg ggtctcgttc
      481 agagtgatcg catacagcca ggtgccgaac gcggcttcgc cgcggaactg gtcgatcctg
      541 tcgatcgcct tgaggaacgt atccnnnnnn nnnntgcctg tcattttgag agccaggttg
      601 tacagcctgg tccggtgggc ggcgatcagt tcggtgaaag cgtcgaaatc gccggcctgg
      661 gcccgccgca ccagctgttg ttctttctcg tccataggat tggtatatac ccggaaagga
      721 tgcagaaaaa caagcgggaa ggcgcggggg cggctgacag tctttgtccg gcccggcgtt
      781 gtggacttcc agggccttct ttttcgaaat cttccggagg cgggaaactc ccgcgcctgc
      841 cgataggtta tggagagtgg caaaatgacc agactcgatg gcccggattg acaactcgtt
      901 tgacaccggc catcaccgct ccgtgtcgtc acccctcccc cggcacggag ctctttttat
      961 gtgcgccggc cccggtctca cgcgagcgtg aagtccgcgg ccgcgactgt cctgccgttc
     1021 acgatgattt cgagacgatg ccgtccgggg acatgtttcc tcaccgacac atcggcgaac
     1081 gactgtctca cctgcagcac aagcgtctcc cccggcgcaa tcgtcttctc ggccagcttg
     1141 aacacttttg gcgacgtctt cccgttcttc ttgacatagt ggacgcggta gtcgatcgcc
     1201 agcttctggc ggctctgtgc gcgcgaggtg acggtgaacg ggaaggcggc gtccgcgccg
     1261 atcgccaccg cccggcgcgg ggggcgcggg ccggtgacgg agattttggg gttggcggtg
     1321 aagccgaaga tgggaaatac ccgcgggtcg ccctttttga tgaggctccg gcaggcgtgg
     1381 cggacaatcc aggtcgtgcc ggcatccccg tctttgagcc agcggagggc ggcggtgatg
     1441 acgaggtcgg ggtggtcttt ggagatgtcg ttgaggttgt tggcgaccgc ggtgcggaca
     1501 tatttcgagg gatcggcttt cagcttctcc agcagggcga ggacggggcg cgggtctttc
     1561 ttgtacgccc cgatgtgcgc aacccagacg cccctcggcc gcgagccttc ggccgccagc
     1621 cgccggacgt gctcgtcggg atcggcggcc cactcgtgga ggatcggcag caggcggtcg
     1681 gggaagctgt tcaggaaggg cctaatcgcc gattctgccg tgctgtagcg ggtgagcgcc
     1741 ttcatgccgc gcaccgaatc ctcgaaatgc gcgaggccct gcagctcgat gaatgtcatg
     1801 gccgcccagt tgtgaaatcc atcgaggcgg ggagcgacgg cgatcagcac gtcgaccgcc
     1861 cggccgtaat cgcccggcag gtattcggcc agggcgcggg caatggcgcg cccccggtct
     1921 ttgaactcga ggcgcggaaa atcatcggtg atgacggaag cgacgaagcc gggaaggtcg
     1981 aaggtcggac agaccgcctt gattgcccgc cccatcgccg tgaccgaggc gcggctgtag
     2041 cggttcttga aaagatcccg ttcgggttcg ggccgggtga ttcggggcat agaagcgaaa
     2101 acctccttgg gacagccgtt ggcgcatggg tgtccccgcg gttagggcga gggcgctccc
     2161 ccggccgcag ccggcggcgc cggccggtga tcttgcggaa gaagcgccgc gagtccccgg
     2221 acatcgtgac cgccggggag ctggaatatg cgcaggtcga acagcggcac ggcagcgagg
     2281 atgtggtcga agaggtcggc ctggagggcc tcgtagttgg tccagatctt gtcgcggctg
     2341 aaggcgtaga tttgcagcgg cagacctttc tcggtcggct cgcggtggcg gacgaggagg
     2401 ggcaggtcgg ggttgatgag cgggtggcgg cgcaggtagg cggagacgta ggcgcggaac
     2461 gtgccaaggt tggtcatgtt gcggccgttg accgggtgcc gtcggtcgat tccgtgggcg
     2521 cggttccact cctcgatttc gccgcggcgc tgccggacgt actcggtaat gagctggaac
     2581 tgctcgaaac gatcgagcat ctcgggcgtg cagaacctga tgctcgacac gtcgatgcag
     2641 accgcgcgca tgattcggcg cccccccgac tcctgcatcc cccgccagtt cttgaaggaa
     2701 tcggcgatga ggtggtacga gggaatgttc gtgatcgtgt tgtcccagtt gcgcaccttc
     2761 acgacgtgca gcgtgacatc gaccacctcg ccgtcggccc cgtacttggg catctcgatc
     2821 cagtcgccga tccggaccat gtcgttggac gacagcaagg tggtcgatgt cacgctgcac
     2881 gtcgtgaagg
//
LOCUS       DAOOKS010000203         2879 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_42348_length_2879_cov_3.011331, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000203 DAOOKS010000000
VERSION     DAOOKS010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42348_length_2879_cov_3.011331"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1072)
                     /locus_tag="PK112_04190"
     CDS             complement(<1..1072)
                     /locus_tag="PK112_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11283.1"
                     /translation="MAHYAALLLSRQALRPCAAAPWVRQTMAALDWLRAEGLTVISSV
                     GMPTWELICAAAGLRGVPQVVIVPGPGGARGTAPDSLCAAFGLGETASFHPLPVDSRK
                     AFPSARDQVVFSLADLLVPISIRPGGFMATRLAEYRSAGRAVVDRFAVAYRPREEPLR
                     YAISERELNPDLEKLSRHYVTHWTRAARGPWPDELPIDYYRSILASPAYPRTALDTLR
                     RILTSRRLVASDRHLPRGVRAVAFSGLSPHRVLPLIRWRARYREMSFEPYGVAIDASV
                     AREHGILPVEYCDPATRPALPPDDHWRLQSSGVVGDWVREEEYRCEGEFDLGTVPEEA
                     LLAFCRFPAEAGDIARTSGVRCL"
     gene            1194..2402
                     /locus_tag="PK112_04195"
     CDS             1194..2402
                     /locus_tag="PK112_04195"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HPC11284.1"
                     /translation="MRKRLVFSVLVLFAALPAAAERLTVERGSDLQSVINYAASGDTI
                     VIAAKTFSARPRLFTDSLCGNCLDPHTPVEASHGFIIRGKSLELIGESRTESRLETNA
                     GYGLFIVDCPEVRIANLTITGGRRDKDGNATDAGIVVRNSRVDISGVTIAGNTHRLDS
                     VVVGIGGVFGREGSEIEIRDCRIVDNGWDGVALYRGAAATVTDCVIRDGRGAGIGVTW
                     DATCAAYRNDISGYWKGIGAFGTSWVIARNNLVHDNLGWGIIATGASYMDIANNVVHH
                     NGNCGVAPWSSEARGRIANNIITANGWREQWVCPCVGVWNYGDWAKWEFRFNIVYGNQ
                     DGNYEAIWDQSGINGNLNGDPLFAGEADFRLTKGSPGINGGDTLIYNADGSRSHIGLE
                     GGPQAKRVRR"
     gene            2568..2657
                     /locus_tag="PK112_04200"
     tRNA            2568..2657
                     /locus_tag="PK112_04200"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2602..2604,aa:Ser,seq:tga)
BASE COUNT          540 a          891 c         1028 g          420 t
ORIGIN      
        1 gcaggcagcg aacgccggag gtccgcgcta tatcgccggc ttcggcggga aaccggcaga
       61 aagccaggag ggcctcctcc ggaaccgtcc ccaggtcgaa ttcaccctcg cagcggtact
      121 cctcctcccg cacccagtcg ccgacgacgc cgcttgattg caggcgccag tgatcatcgg
      181 gcggcagcgc gggccgggtt gcgggatcgc agtactcaac cgggagaatg ccgtgctcgc
      241 gggcgaccga ggcgtcgata gcgacgccgt aaggttcgaa tgacatctcg cgataccggg
      301 cgcgccagcg tatcagcggc agcactcggt gcggggacag gccggagaaa gcgacggcgc
      361 gcacaccccg gggcaaatgg cggtccgacg caacgagacg ccgcgatgtc agaatcctcc
      421 tcagcgtgtc caatgcggta cgggggtagg cgggggaggc caggatgctc cgatagtagt
      481 cgatcgggag ttcatccggc catggtccgc gggccgcacg cgtccaatgg gtgacataat
      541 gacgagagag cttctcgaga tcagggttca gctcgcgttc ggagatcgca taccgcaacg
      601 gctcttcccg agggcggtag gcgacggcga agcggtcgac gaccgcgcgg cccgccgacc
      661 ggtactccgc cagccgggtc gccatgaaac cgccggggcg gatggaaatg gggaccagaa
      721 ggtcggccag gctgaaaacg acctggtccc gggcggatgg gaaggccttg cgcgagtcga
      781 ccgggagcgg gtgaaaggag gcggtctcgc cgagtccgaa ggcggcgcag agactgtcag
      841 gcgccgtgcc gcgagcccca cccggtcccg gaacgatcac gacctggggc acaccgcgca
      901 gaccggcggc ggcacaaatc aactcccagg taggcatccc cacagagctg atcacagtca
      961 gtccctcggc gcggagccag tcgagggcgg ccatggtttg ccgcacccac ggagcggcgg
     1021 cgcacggccg aagcgcctgg cgggacagca ggagagcggc atagtgggcc atcagcggga
     1081 atctgggcgg cgcgcggggc gcggggaatc acattctgcg gccgcggcgg tggcccggcc
     1141 tcccggcggc tttttgcttg cccgccgccg ccgcgcagcg cttaatacgg cgtatgcgaa
     1201 agagactggt gttcagtgtg ctcgtgcttt tcgcggctct gccggcggcg gcggagcgcc
     1261 tcaccgtgga acgcggctcc gacctgcagt cggtgatcaa ctacgccgcg agcggcgaca
     1321 ctattgtaat cgcggcgaag actttcagcg cccgcccgcg cctgtttacc gactcgctct
     1381 gcggaaactg cctcgatccg cacacgccgg tggaggcgtc ccacgggttc attatccgcg
     1441 gcaagtcgct cgagctgatc ggagaaagcc gcaccgagag ccggctggag accaacgccg
     1501 gctacgggct gttcattgtc gactgcccgg aggtgcgcat cgccaacctc acgatcaccg
     1561 ggggacggcg ggacaaggac gggaacgcga ccgacgccgg gatcgtggtg cgcaactcgc
     1621 gcgtcgacat ctccggcgtc accatcgcgg gcaacacgca ccggttggat tcagtcgtgg
     1681 tcggcatcgg aggcgtgttc ggacgggagg gctccgagat cgagatccgg gactgccgga
     1741 ttgtcgacaa cggctgggac ggcgtggcgc tctaccgggg cgcggcggcg accgtgaccg
     1801 attgcgtgat acgggacggc cggggcgccg ggatcggcgt gacctgggat gcgacctgtg
     1861 cggcctaccg gaacgacatc agcggctact ggaaaggaat cggggcgttc ggaacctcct
     1921 gggtgatcgc gcgcaacaac ctcgtgcacg acaatctcgg gtggggaatc atcgcgaccg
     1981 gcgcctcgta catggatatt gccaacaatg tcgtgcacca caacggcaac tgcggagtgg
     2041 cgccctggtc gagcgaggcc cgcggccgga tcgccaacaa tatcatcacc gccaacggct
     2101 ggcgcgagca atgggtgtgt ccgtgcgtgg gagtctggaa ctacggcgac tgggccaagt
     2161 gggagttccg gttcaacatc gtctacggca accaggacgg caactacgag gcaatctggg
     2221 atcagtccgg catcaacggg aatctcaacg gcgacccgct gtttgcggga gaggcggatt
     2281 tccgtctcac caagggatcc ccgggtataa atggcggcga tacgctgatc tataacgccg
     2341 acggcagccg ttcgcacatt ggccttgagg gcggcccgca ggcaaaacga gtgcggcgct
     2401 gactgcgtaa gagcgccggg acacggccgc gccgggtgca gcgcaggaaa ggtgcggaac
     2461 cggggcgggc aatccgctga accggacgct tggacgacga gggggttcgg aagacggacg
     2521 cagggcggga agggcggccg aagtggacga gacagacata acggaacgga ggagtggctg
     2581 agtggtcgaa agcggcggtc ttgaaaaccg ttgtgcccgc gagggcaccg tgggttcgaa
     2641 tcctacctcc tccgccatat ttcagttttg ggctcttcgg caaggtagct gaagggcttc
     2701 tcgtaagtgg gggagaggcc tcagtcggtg agcgtgaagt tcgaaggctc cataccctcc
     2761 gccacccctt cgccggccac ctggtcatgt ccggggtcga cctgcccacg gtcaagaagc
     2821 tcatgggcca ctcggacatc ccgacgaccg tgatctacgc ccacctcgcc gaggcggtg
//
LOCUS       DAOOKS010000204         2865 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_42634_length_2865_cov_2.748754, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000204 DAOOKS010000000
VERSION     DAOOKS010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42634_length_2865_cov_2.748754"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="PK112_04205"
     CDS             complement(<1..237)
                     /locus_tag="PK112_04205"
                     /inference="COORDINATES: protein motif:HMM:NF039825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5684 domain-containing protein"
                     /protein_id="HPC11285.1"
                     /translation="MYQSGYTDGGMAFGAFLWLFVAAFYLFFAYAQYRIAKKCGHDSE
                     AWWGFIPILNIFLLLKCAGREWYWFLFLLVPIVNI"
     gene            564..1736
                     /locus_tag="PK112_04210"
     CDS             564..1736
                     /locus_tag="PK112_04210"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HPC11286.1"
                     /translation="MDTLVKAKAAQATALLGELGIDLWLIFVRETKMMADPALALVVG
                     EEATWQSCFAFSRNGEAVALVGNLDAANFERAGQFSEVRTYTHGVGEDLAGLVRRFDP
                     AQIAVNYSANDPAADGMTHGMYLLLKEHLRGTPYGERLVSAERLMSKLRSRKLPEEVA
                     RIATAAQLTAAVWESEAPHVRAGMTEAEVAGLIDRGIAGAGGEPSFATIVNAGDKSAP
                     GHGLPSGAKLAGGDLLHVDFGIRREGYCADLQRLLYFRRPGEKRPPAELREAFAMVRD
                     IITETAKAARPGATGCAVDALAREMLRDHGYPEYQHALGHQLGRDVHDGGGILGPEWE
                     RYGVTPRMELEAGNVFTLELEILLPGIGCVGLEEDAVIREDGAEFLCPRQMELAVR"
     gene            1733..2587
                     /locus_tag="PK112_04215"
     CDS             1733..2587
                     /locus_tag="PK112_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11287.1"
                     /translation="MKLRAAAMIAAAALAAGCGGPQAPPPPEAAATPDRTSVCAPAEV
                     RAEASAGTAEISFRASCPTAISGYNIYISTVPLGEAYPGTALPGSVEAWNHPVFPGDT
                     TPEDGVEVYRAEGLTDGVRYYVHVRTVFPDQSLSRPSEEIAIVTGPRGTIELAARFAS
                     EQDGFSFAAGAYVRADDLANDLYFYPAEGRDFLASPDRLGLLRATRLRVLPFRGELSE
                     AAAGAATMSSLPTEPRVAVEEGDWVQVMTADKTFALVKVLGLEGQGEGRKMRLFYVWS
                     PAVGAPVF"
BASE COUNT          485 a          798 c         1116 g          466 t
ORIGIN      
        1 gatgttgact atgggcacca gcaggaacag gaaccagtac cattcgcggc cggcgcactt
       61 gagcagcagg aagatgttga ggatcggaat aaacccccac caggcctcgc tgtcgtgacc
      121 gcacttcttg gcgatgcggt actgcgcata cgcgaagaag agatagaacg cggcgacgaa
      181 gagccacaaa aaggcgccga acgccatgcc gccatcggtg taaccggatt ggtacattct
      241 tctttcctcc tcaggagatt agggataaag cgctattcgg gttcgcggtg attcgaagcc
      301 gccgccgggg tttgctcctg ttctcgttat ttgtcggtct tgtccgtcaa gcgccctacg
      361 cagcggccga cctcccgtcc ttctcatgcg gccggtggcg ccgcaggtat cgggtctcac
      421 ctgtaagatc gacagatttt gtcgaatctt gattgccggg tggacgggag tgcgcagaaa
      481 ttgaacaggg gacgccggtc atggccgaag cggcggggat ttatcggctt gtgcgggggc
      541 gggagagcgg ttagtttagc aggatggata cgctggtcaa ggccaaagcc gcccaggcaa
      601 cggcgctttt gggggagctg gggatcgatc tctggctcat ttttgtgcgg gagaccaaga
      661 tgatggccga cccggcgctg gcgctggtgg tcggggagga ggcgacctgg cagtcgtgtt
      721 tcgcgttctc ccggaacggc gaggcggtgg cgctggtggg aaacctggat gcggcgaatt
      781 tcgagcgggc cgggcagttc agcgaggtgc gtacttacac ccacggggtg ggggaagatc
      841 tggcggggct ggtgcgccgg ttcgacccgg cgcagatcgc ggtcaactac agcgccaacg
      901 atcccgcggc ggacggcatg acgcacggga tgtacctgct gctgaaggag catttgcgcg
      961 ggacgcccta cggggagcgg ctggtctcgg cggaacggct gatgagcaag ctgcggagcc
     1021 ggaagctgcc ggaggaggtg gcgcggatcg ccacggccgc ccaattgacg gcggcggtgt
     1081 gggagtcgga ggcgccgcac gtgcgggcgg ggatgacgga ggcggaggtg gcggggctga
     1141 tcgaccgcgg gatcgccggg gccgggggag aaccttcgtt tgccacgatt gttaatgcgg
     1201 gggacaagag cgcgcccggc cacgggctcc cttcgggggc caaactcgcg ggcggagact
     1261 tgctccatgt cgatttcggc attcgccgcg aagggtactg cgcggacctc cagcggctgc
     1321 tctactttcg gcggcccggg gaaaagcgcc cgccggcgga gctgcgggaa gcatttgcga
     1381 tggtgaggga cattattacg gagacggcga aagcggcgcg gccgggcgcg accgggtgcg
     1441 cggtggatgc gctcgcgcgc gagatgctcc gcgaccacgg ctacccggag taccagcacg
     1501 cgctcggcca ccagctcggc cgggatgtcc acgacggcgg cgggatcctt ggaccggagt
     1561 gggaacggta cggggtgacg ccgcggatgg agctcgaggc cgggaatgtg ttcacgctgg
     1621 aactggaaat cctgctgccg ggaatcggct gcgtcggact ggaggaggat gcggtgatcc
     1681 gggaggacgg ggcggagttc ctgtgcccgc gacagatgga gttggcggtg cgatgaagct
     1741 gcgggcggcg gcaatgatcg cagcggcggc gctggccgcc ggctgcggcg ggccgcaggc
     1801 gcctccgccc ccggaggccg ccgcgacccc cgaccggact tccgtctgcg ctccggcgga
     1861 ggtgcgcgcc gaggcgagcg ccgggacggc cgagatcagt ttccgggcat cctgcccgac
     1921 cgccatctcc gggtacaaca tctacatcag cacggtccct ctgggcgagg cctatccggg
     1981 gacagcgctg ccgggatcgg tcgaggcctg gaaccatccc gtgttccccg gcgacacgac
     2041 gccggaggac ggcgtggagg tgtaccgggc tgaagggctg acggacgggg tccgatacta
     2101 cgtacatgtg cggaccgtct ttcccgacca gtcgctctcg cggccctcgg aggagattgc
     2161 gatcgtcacc ggcccgcgcg ggacaattga actggcggcc cgcttcgcct cggaacagga
     2221 cggcttttcg ttcgcggccg gggcatatgt ccgggcggac gacctggcga acgacctgta
     2281 cttttatccg gcggaaggac gggactttct cgcctcgccg gaccggctgg ggttgctccg
     2341 ggccacccgc ctgcgggtgc tgccgttccg gggggagctg agcgaggcgg cggccggcgc
     2401 ggcgacaatg tcatcgctgc ccaccgagcc ccgggtggcg gtcgaggaag gggattgggt
     2461 gcaggtcatg acggcggaca agacgttcgc gctggtgaaa gtgctgggac tggaggggca
     2521 aggagagggg cgaaagatgc ggttgttcta tgtctggtcg ccggcggtgg gggcgccggt
     2581 gttctgagac cggggccgga ggccgggctc gcgcgaggac cgagcagaaa gatggggagt
     2641 ccggcgggcg ggcaaagagc gggctgcggc gagcggaaaa gccggaacgg aaaattggag
     2701 atgcgataga cgagggtttt gccttggaat ttcacaaaga cagtctgaag attgatgctg
     2761 cgcgagtggc ggagtcgctg cggcgggtga tcgccgagca gatcggggtg cagatgaaga
     2821 agcgcggggc ggtggtgggt atctccggcg gaatcgattc ctcgg
//
LOCUS       DAOOKS010000205         2856 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_42809_length_2856_cov_3.214209, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000205 DAOOKS010000000
VERSION     DAOOKS010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42809_length_2856_cov_3.214209"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..693)
                     /locus_tag="PK112_04220"
     CDS             complement(25..693)
                     /locus_tag="PK112_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11288.1"
                     /translation="MVCHGGWSGCELFAVGGFSALLVLVARVSPAYGCFVLVAAAPLL
                     AAVARASVRDAACLFALFALTLVGVGQLDLIRLSPLVWGLRMAAAGAVGCAFGLLFGL
                     ANRRFGFHPLVLALLWVGFELTIVQTGIAPSVLGLHAPSGAVTLELSQLFGFAIVSLL
                     IILLDSAILWSLEKIFSAEARGTNERVNGRIWNLLFTPRPLAGRFFLVPHRRGPPPAT
                     SPQH"
     gene            complement(1161..1667)
                     /locus_tag="PK112_04225"
     CDS             complement(1161..1667)
                     /locus_tag="PK112_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11289.1"
                     /translation="MRRYLILLVGAACVLLACGRGVETIAEFPVDNLDGIIAQSNVTL
                     DREITADGGGALRIEAPAAATIPLLELVDPDVEKATLTFEAKIRTQNLTGMVYLEMLC
                     HFPERGEFFARGLDNPVTGDVDWSSQSTQFFLKAGENPDLVKLNIGVAGSGTIWVDAI
                     KVTRGPLK"
     gene            complement(1788..2426)
                     /locus_tag="PK112_04230"
     CDS             complement(1788..2426)
                     /locus_tag="PK112_04230"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HPC11290.1"
                     /translation="MAERRQTNEEIEIKLDLGSFTNYLKLVGFLGQLDGEERQVNAFF
                     DTEDRVLSRDGWALRVRVDEQRGSLAIKSRETAGEEASIRMEMESEIPRGEALEALAL
                     QRDIMGMDTPTIQFVRDQFGSLALVRFVHFENTRQYKAFRFGDYSYRLEIDTTRYADG
                     SVDYELEVELPDRTHVPTVIDRLRKLFLSLDIPFLRQNRSKLARALERAPRA"
     gene            complement(2564..>2856)
                     /locus_tag="PK112_04235"
     CDS             complement(2564..>2856)
                     /locus_tag="PK112_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11291.1"
                     /translation="PTCEGLGRILSKETMATKIERWFLRAKAEGRIREFNLALNPMLA
                     DAMSENGHSRIDRLMRINQFRINVVRDTTLSVQEYRVYDAQNNDNITERYRL"
BASE COUNT          573 a          848 c          956 g          479 t
ORIGIN      
        1 ctcggatgaa cttactgcag cacgtcagtg ctgcggggaa gttgccggcg ggggaccgcg
       61 gcgatgcggg acgaggaaga acctcccggc gagagggcgg ggggtgaata aaaggttcca
      121 gattctccca ttcactctct cgttagttcc ccgcgcctcc gccgagaata tcttctcaag
      181 gctccagaga atggccgaat ccaggagaat gatgaggagg gagacgatcg caaagccgaa
      241 gagctgcgac aactccaggg tcacggcgcc cgacggggcg tggagcccga gcaccgaggg
      301 ggcaataccc gtctggacaa tagtcagttc gaatccgacc cacaacagcg cgagcacgag
      361 cggatggaat ccgaagcgac gattggccag accgaaaagc agcccgaacg cacaccccac
      421 tgcgcccgcc gcggccatcc gaagccccca gaccaggggg gacagccgga tcaggtcgag
      481 ttggccgacg ccgaccaggg tgagagcgaa aagggcgaag agacaggcgg catcacggac
      541 ggaggcgcgg gcaacggcgg ccagaagggg agcggcggcg accaggacga aacagccgta
      601 ggccggcgat acccgggcta caagcaccaa cagcgccgag aagccgccga ccgcaaacaa
      661 ttcgcagccc gaccagccgc cgtggcacac cacggcccgg cgctctccct tggcgacacg
      721 ggtacaaggc gaagaagttg gctcaatggt gtggttgtct gaacattgag acgaggtgcc
      781 tgcgcggagt cgatcgagac agtcgtccgt tgcgccgagg cgcaattatc cacctccctg
      841 gaagcgggca aacccgaccg gcccgcctca gttcgacgac gaattgatct ccgagacaat
      901 ggaaaaagct ggaacccgta ctacaactgg aaaatacgcg gcctgacagg ggctgtcaag
      961 gaaattctcg cgcaattgaa ccctgggcgc acgcaatgcg ccggattccg ccggtcctct
     1021 atggatcacc gaatcatccg ggcacctcgt gcaccggtga ccggccgcgc cgatcccgcc
     1081 gggccgagag gccgggcggc gaatgcgaat gccgtctggg acaatcccgg gcggatggcg
     1141 cgcccgggaa taggatcgct ctacttgagc gggccccggg tgactttgat ggcgtcgacc
     1201 cagatcgtgc cggagccggc gacgccgatg ttgagcttca ccagatccgg attctctccg
     1261 gctttgagga agaattgcgt gctctgggag ctccagtcga catcgcccgt gaccgggtta
     1321 tcgaggccgc gggcgaaaaa ctcgccccgt tcggggaagt ggcagagcat ttccaggtac
     1381 accatgccgg tgagattctg cgtgcgaatc ttggcttcga acgtgagcgt ggccttctcg
     1441 acatcgggat cgaccagttc cagcagcgga atggtggcgg cggcgggcgc ctcgatccgg
     1501 agagcgccgc cgccgtcggc cgtgatctcc ctgtcgagcg tgacgttact ctgggcgatg
     1561 atgccgtcga gattgtccac cgggaattcg gcgatcgttt ccacgccgcg accgcaggcg
     1621 agcaggacac acgcagcgcc gaccagtaga atcagatacc gtcgcatggg cgaaacctca
     1681 tcgatataag agtcattttg ttctgaattg tccgatcaca aagacatcgg tattctgcca
     1741 tcggcaggat cgacaggcaa gtttaatcgg gatgggcggc cagggagtca ggcccgaggg
     1801 gctctctcga gggcccgggc gagtttggag cggttctgcc ggaggaaggg gatatccagc
     1861 gacaggaaga gttttctgag tcggtcgatg acggtgggga catgggtgcg gtcgggcaac
     1921 tcaacttcga gctcgtagtc gaccgagccg tcggcgtagc gcgtggtgtc gatttcgagg
     1981 cgataggagt agtcgccgaa cctgaacgcc ttgtactggc gggtgttttc gaagtggacg
     2041 aagcggacga gagcgaggga cccgaactga tcccggacga actggatggt gggcgtgtcc
     2101 attcccatga tgtcgcgctg cagggcgagg gcttcgagag cctccccgcg ggggatttcg
     2161 gactccatct ccatgcggat agaggcttcc tcgccggccg tctcacggct cttgatcgca
     2221 agcgagccgc gctgctcgtc gacccgcacc cggagcgccc agccgtcgcg cgacagcacg
     2281 cggtcttcgg tatcaaagaa cgcgttgacc tgccgctcct cgccgtcgag ctgccccaaa
     2341 aagcccacga gcttgaggta gttggtgaag gatccgagat cgagcttgat ctcgatttcc
     2401 tcgtttgttt gtcgccgttc tgccatcctg ccgccaataa acgcaatccc ggggcgcggt
     2461 tcaatggaaa ttttgcggcc cggtcggccg gggggcggcc gcaaaaaagc ggcgtcggat
     2521 ggcgatccga cgccgcggag atctccgcca ggtcccccgg ggctcagaga cgatagcgtt
     2581 cggtgatatt gtcgttgttc tgggcgtcgt agacgcggta ctcctgcacc gagagcgtcg
     2641 tgtcgcggac gacattgatg cggaactggt tgatgcgcat gaggcggtcg atccggctgt
     2701 ggccgttttc cgacatggcg tcggccagca tcgggttgag ggcgagattg aactcgcgga
     2761 tccggccctc ggccttggcc cggagaaacc accgttcgat tttcgtggcc atcgtctcct
     2821 tcgacaggat acggcccagc ccctcgcagg tcggac
//
LOCUS       DAOOKS010000206         2851 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_42925_length_2851_cov_4.092632, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000206 DAOOKS010000000
VERSION     DAOOKS010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42925_length_2851_cov_4.092632"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..852)
                     /locus_tag="PK112_04240"
     CDS             complement(<1..852)
                     /locus_tag="PK112_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11292.1"
                     /translation="MMETPNTDRPPDQEIAVLLRDLLRTIKAVCMYPETNSLPQSLRQ
                     SFASRLLDLLEEQGEIRLGVDNGVILRNGEIVHRDKSREDNLAAIFFSAGITELSFKR
                     GLTAASIDRLLGAIRTYLTAPAGHGDLPSLLWEAAPEGFSFQSAEDVALAEYDAGFRV
                     QEYFHREDERRRAGGDTPDGYEAIFLSEDALEGRAAPETVDGHMTPGGVFEVEPEEAA
                     RLRAQAAAEAMGLSNLPPVPVPSVRDILSRENSLSADEQARMEQLLAQDAAFNLYEST
                     SHLLIELL"
     gene            complement(979..2328)
                     /locus_tag="PK112_04245"
     CDS             complement(979..2328)
                     /locus_tag="PK112_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HPC11293.1"
                     /translation="MAKKSAPKQSGVSGHRQLMEKITSKKAKAGVIGLGYVGLPLAVE
                     LVHAGFEVTGFDIAEPKVRLLNSGRSDIDDVPDAVVADMVAAKKFRATTDFRALGEMD
                     TISICVPTPLSKTKDPDVSFILAAVGSVKQFMRKGTLVVLESTTYPGTTEDLILPLLQ
                     EKGMKAGEDFFLAFSPERVDPGNQRFTTKNTPRVVGGITPKCTQVAKTFYEQSIPDIY
                     AVSSTQAAEMVKLLENTFRSVNIGLVNEVALMCDRLGLNVWEIIDAAATKPFGFMPFY
                     PGPGLGGHCIPIDPHYLAWKLKSLNYYARFIELAGDINSSMPHYVVDRVMRMLNERFA
                     KALNKSSVLVLGIAYKANIKDVRESPALDVIKLLEDRGARVVFNDPHVPSIQWEGTAR
                     RSTRLTAALLRKTDLVVIVTNHAQYDYQWIVDNAKAVFDTRNATRKVRNGRKKIEVL"
     gene            complement(2348..>2851)
                     /locus_tag="PK112_04250"
     CDS             complement(2348..>2851)
                     /locus_tag="PK112_04250"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HPC11294.1"
                     /translation="HLCRSHFRRYGLPVTVARSFNHSGPRQAPIFVIADFARQAAAID
                     LGLQPPRVRVGNLAARRDFSDVRDIVRGYRLLAERGAPGEVYQLCSGRATSIETVLRL
                     LLGFTAKPVAIAVDRGKWRAADIPVLRGSNRRAVEELGFANRYSLKQTLRETFAYWKE
                     RLSAENE"
BASE COUNT          478 a          962 c          889 g          522 t
ORIGIN      
        1 gagcagctcg atgaggaggt gcgacgttga ctcgtacagg ttgaaggcgg catcctgcgc
       61 cagcagttgc tccatccggg cctgctcgtc ggcgctgagg ctgttctcgc gggagaggat
      121 gtcgcgcacg ctgggcaccg gcaccggggg cagattggac agccccatgg cttccgccgc
      181 cgcctgcgcc cggaggcggg cggcctcctc cggctcaacc tcgaacacgc cgccgggtgt
      241 catgtgcccg tccaccgtct ccggcgccgc tctcccctcg agcgcgtctt cgctcaggaa
      301 tatcgcttca tacccgtcgg gcgtatcgcc ccctgcccgg cgccgctcgt cctcccggtg
      361 gaagtactcc tggacgcgga aaccggcgtc atactcggcc agggccacat cctccgccga
      421 ctggaacgag aaaccctcgg gcgccgcttc ccacagaagc gacgggagat cgccgtggcc
      481 cgcgggcgcc gtgagataag tccggatcgc tccgagcaga cggtcgatgc tcgccgccgt
      541 cagcccccgc ttgaaactga gttccgtgat gccggcgctg aaaaagatgg cggcgagatt
      601 gtcctcccgc gacttgtcgc ggtgcacgat ttccccgttg cgcagaatga ccccgttgtc
      661 gacccccagc cggatctccc cctgctcctc gagcaggtcg agcagccggc tggcgaatga
      721 ctgccggagc gactgcggga gcgaattcgt ctccgggtac atgcagacag ctttgatcgt
      781 ccgcaggaga tcccgcagga gaacggcgat ttcctgatcc ggagggcggt cggtattcgg
      841 tgtttccatc atcctgccgt tggttttctg atcacagtat cggcagaatg ccccgcccga
      901 ttgaccggcc gccgggccgg tcaacgaaaa acccggcggc gccgggtttt ccgtcaaagc
      961 ctccggtcgg tcaagcgctt acagcacttc gatcttcttc cgcccgttcc gcactttccg
     1021 ggtcgcgttg cgggtgtcga ataccgcctt ggcgttgtcc actatccact ggtagtcgta
     1081 ctgggcgtgg ttggtcacga tcaccaccag gtcggtcttg cgcagcagcg cggccgtcag
     1141 ccgggtggac cgccgggccg tcccctccca ctgaatcgac gggacgtgcg ggtcattgaa
     1201 caccacccgc gcgccgcggt cctccagcag cttgatgacg tccagcgccg gcgactcccg
     1261 cacatccttg atgttcgcct tgtaggcgat ccccagcacg agcaccgagg acttgttgag
     1321 cgccttggcg aaccgctcgt tgagcatcct catcacccgg tcgaccacgt agtggggcat
     1381 cgaggagttg atgtcccccg ccagctcgat gaaccgggca tagtagttca gcgacttcag
     1441 cttccacgcc aggtagtggg gatcgatcgg gatgcagtgc ccgccgagcc ccggaccggg
     1501 atagaacggc atgaacccga agggcttggt cgccgccgcg tcgatgatct cccacacgtt
     1561 cagcccgagc cggtcgcaca tcagcgccac ctcgttcacc aggccgatat tcaccgagcg
     1621 gaaagtgttc tccagcagct tgaccatctc cgccgcctgg gtcgacgaca cggcgtagat
     1681 gtccgggatc gactgctcgt agaatgtctt ggccacctgg gtgcacttgg gcgtgatccc
     1741 cccgaccacc cgcggcgtgt ttttcgtcgt gaaccgctgg ttccccggat ccacccgctc
     1801 gggcgagaag gcgaggaaga aatcctcccc cgctttcatc cccttctcct gaagcagcgg
     1861 caggattagg tcctccgtcg tgcccggata agtggtcgat tcgagcacga cgagcgtccc
     1921 tttgcgcatg aactgcttga ccgagccgac cgcggccagg ataaacgaca cgtcgggatc
     1981 tttcgtcttc gacagcgggg tcggcacgca gatcgaaatg gtgtccatct cgccgagagc
     2041 gcggaagtcg gtggtggcgc ggaacttttt ggccgccacc atgtcggcca ccacggcatc
     2101 gggcacgtcg tcgatgtccg accgtccgct gttgagcagc ctgactttcg gctcggcaat
     2161 gtcaaaaccg gtgacctcaa acccggcgtg caccagctcc accgccaggg gcagtccgac
     2221 gtagcccaga ccgatcacac cggccttggc tttcttgctc gtgatcttct ccataagctg
     2281 ccggtgaccg gacacgccgg actgcttggg agctgatttc ttggccatac aggattactc
     2341 catcacctta ttcattctcg gcggacagac gttctttcca gtacgcgaac gtctcccgca
     2401 gcgtttgctt cagactgtat cggttggcaa atccgagttc ttcaacggcc cggcggttgg
     2461 atccgcgcag gacggggatg tcggcggcgc gccactttcc ccggtccacc gcgatggcga
     2521 cgggtttcgc cgtgaacccg agcaggaggc ggaggacggt ctcgatcgag gtcgcccgtc
     2581 cggagcacaa ctggtagact tcgcccgggg cgccgcgttc ggccaacagg cggtaaccgc
     2641 ggacgatgtc gcggacatcg ctgaaatccc gccgggccgc gagattcccc acgcgcaccc
     2701 gcgggggctg cagccccagg tcaatggccg ccgcctgacg ggcgaaatcg gcgatcacga
     2761 aaatcggcgc ctgccgcgga ccgctgtggt tgaacgaccg cgccaccgtg accggaaggc
     2821 cgtagcggcg gaaatgcgag cggcagagat g
//
LOCUS       DAOOKS010000207         2847 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_43029_length_2847_cov_2.773281, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000207 DAOOKS010000000
VERSION     DAOOKS010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43029_length_2847_cov_2.773281"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1269)
                     /locus_tag="PK112_04255"
     CDS             complement(<1..1269)
                     /locus_tag="PK112_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11295.1"
                     /translation="MRNPLRPVAAAAALVLLAALPQAARAQQPALVIGEVTFPSLRWG
                     QQSAQVPVTNSSDYLKFIVAETDIRFTGAYLNPERRIRSYHALPPGESTTIEAAVLVP
                     GNFGQAQVYLRLYDVVDTLDEILPGQKFYEQPFLVTFHVPEGLRPYVDTRVTLPPRVE
                     DHPYFDNEFARLLFLLLGEGKLPAEIAALAECDTGFVSEQLALMVGEGYVGKDSTGFK
                     LKIPVIRAAEADSARSRALVLADSLTDLIAANLPRFAAVRDSLVAAGRVNRDSNAFFD
                     PGTVLYHTYPVVGGLALWWDLGNAFITRSAPLYLYDGTDLCNADIPIYMYAVEGGPVV
                     NGHLFYAPVVSSTSYELYFGDTIPTIACDPDFIRFKSYPIHVNFQPAEEYRPTTYVFD
                     SAVVRPAVEALTYGADSLLAHAYLDLRDMAV"
     gene            complement(1262..2311)
                     /gene="rlmN"
                     /locus_tag="PK112_04260"
     CDS             complement(1262..2311)
                     /gene="rlmN"
                     /locus_tag="PK112_04260"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HPC11296.1"
                     /translation="MPKVNLLGYTKPQLEALMTSLGQKPYKGRQMFNWLYKVRQHDFQ
                     LMTDLSKEVRARLDEHYTFRLMQPELESVSEDGTRKFLFRLEDGHPIETVFIPDDGRA
                     TVCISVQAGCALACRFCATGTLGLLRDLTPGEIVGQLMFLRERFGGEAFTNIVMMGMG
                     EPLNNFAAVVDAIRIMTDPSGLCHAARRITISTSGVSPKIRKLADLGLKTRLAVSLHA
                     AIQEKRVQLMPVAETFKLDKLMEAVRYYALAARDRVTFEYILFKGFNDTPEDVKALTR
                     LVHGIPCKINLLAYNPVPGLPFARPTDEEVDAFARLLYPRVPAVTVRKSRGRDIDAAC
                     GQLAARAAARRTDHA"
     gene            2459..2533
                     /locus_tag="PK112_04265"
     tRNA            2459..2533
                     /locus_tag="PK112_04265"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2494..2496,aa:Pro,seq:tgg)
BASE COUNT          534 a          806 c         1027 g          480 t
ORIGIN      
        1 gaccgccatg tcgcgaagat caaggtacgc gtgcgccaag agcgaatcgg ccccgtacgt
       61 gagcgcctcg accgccgggc gaacgaccgc gctgtcgaaa acataggtgg tcggacggta
      121 ctcctcggcg ggctggaagt tgacgtggat cgggtaggac ttgaagcgga tgaagtcggg
      181 gtcgcaggcg atcgtgggaa tggtgtcgcc gaagtagagc tcgtagctcg tactcgaaac
      241 gaccggggcg taaaagaggt ggccgttgac caccgggccg ccctcgaccg catacatgta
      301 aatgggaatg tcggcgttgc agaggtcggt gccgtcgtag aggtagagcg gcgcggagcg
      361 ggtgatgaaa gcgttgccga gatcccacca gagggcgaga ccgccgacca cggggtaggt
      421 gtggtagagg acggtgccgg ggtcgaagaa ggcgttgctg tcgcggttga cgcggccggc
      481 ggcgacgaga ctgtcgcgga cagccgcaaa gcgcgggagg ttggcggcga tgagatcggt
      541 cagagagtcg gccaggacca gagcgcggga gcgggccgag tcggcctcgg cggcgcgaat
      601 gaccggaatt ttgagcttga atccggtcga gtcctttccg acatacccct cgcccaccat
      661 cagggcgagc tgctcactga cgaatccggt gtcgcactcg gcgagcgcgg cgatctccgc
      721 cgggagtttg ccctcgccca ggaggaggaa caggaggcgg gcgaattcgt tgtcgaagta
      781 ggggtggtcc tcgacccggg gcggaagggt gacgcgggtg tcgacatacg gccgcagccc
      841 ctcgggtacg tggaaagtga ccaggaaggg ctgttcgtag aatttctggc cgggcaggat
      901 ttcatcgaga gtgtcgacca cgtcgtagag acgaaggtac acctgggcct ggccgaagtt
      961 gccggggacc aggacggcgg cctcgatcgt cgtggattcg ccgggcggca gggcatggta
     1021 ggagcggata cggcgctcgg ggttgaggta ggccccggta aagcggatgt cggtttcggc
     1081 gacgatgaat ttcaggtagt cggagctatt ggtgaccggg acctgggccg actgctgccc
     1141 ccaccgcagg gaggggaaag tgacctcgcc gatcacgagc gcgggctgct gggcgcgggc
     1201 ggcctgcgga agggcggcga gcaacaccag ggcggcggcc gccgcgacgg ggcggagcgg
     1261 attacgcatg atcagttctc cttgcggcgg cgcgggcagc caactgcccg cacgcggcgt
     1321 cgatatctct cccccggctc ttgcggacgg tgacggcggg cacccgcgga tagagcaggc
     1381 gggcgaaagc gtcgacctcc tcatccgtgg gccgcgcaaa gggcaggccg ggaaccgggt
     1441 tgtaggcgag caggttgatc ttgcagggaa tgccgtggac cagccgggtg agggctttga
     1501 cgtcctccgg cgtgtcgttg aagcccttga agaggatgta ttcgaaagtg acgcggtccc
     1561 gggcggccag ggcgtagtag cgcaccgcct ccatgagctt gtcgagtttg aacgtctcag
     1621 ccacgggcat caactgcacg cgcttctcct gaatggcggc gtggagagag accgcgaggc
     1681 gggtcttgag tccgaggtcg gccagtttgc ggattttcgg cgagaccccc gaggtcgaga
     1741 tggtgatgcg ccgggcggcg tggcagagac cgctcgggtc ggtcatgatg cggatcgcgt
     1801 cgaccaccgc ggcgaaattg ttgagcggct cccccatgcc catcatgaca atgttcgtga
     1861 acgcctcgcc gccgaaccgc tcgcggagaa acatgagctg gccgacaatc tcgccgggag
     1921 tgaggtcgcg cagcaggccg agcgtgccgg tggcgcagaa ccggcaggcc agcgcgcagc
     1981 cggcctggac agaaatgcag accgtggccc ggccgtcatc gggaatgaaa accgtctcga
     2041 tcggatgccc gtcctccagc cggaacagga atttgcgcgt gccgtcctcg gaaaccgatt
     2101 cgagctcggg ctgcatgagg cggaacgtgt agtgctcgtc gaggcgggcg cgcacctcct
     2161 tggagaggtc ggtcatgagc tggaaatcgt gctggcggac tttatacagc cagttgaaca
     2221 tctggcgccc cttgtacggt ttctggccga gggacgtcat gagagcttcg agctgcggtt
     2281 tcgtgtatcc gagaaggttg acttttggca tcgctgataa atttacctga tggcgtcaaa
     2341 atggcaagcg ggaattggcc gccgccggct caacaggtgt acccacgggg gaaaggttgg
     2401 ttccgggatt ggcgattgac agccggccgg gggagaaggt attttgagaa atgagaatcg
     2461 gggtgtagcg cagcccggtc agcgcgcctg ctttgggagc aggatgtcgg aggttcgaat
     2521 cctctcaccc cgatgtacat gcgggagcat ctcagcaaac agccccgtca ggatcctgaa
     2581 ggtcctgatc gacaaaaact ccgcccgcca tgatctcctg taggccagga cccttgtggt
     2641 cctgacacct tcgctttctt cgcaaacgac cccgtcaaaa ccatgaaggt cctgacctac
     2701 aaaaagcccg ctgcgcgatg atctcctgta ggccaggacc cttgtggtcc tgacatactt
     2761 gctccgctcg gctcaacgcc cgggcgccga tcggctgtcc gacctcacgg gcacgccggc
     2821 ggcgcctctc ccccccggaa caggtac
//
LOCUS       DAOOKS010000208         2844 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_43092_length_2844_cov_3.872714, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000208 DAOOKS010000000
VERSION     DAOOKS010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43092_length_2844_cov_3.872714"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..961)
                     /locus_tag="PK112_04270"
     CDS             complement(<1..961)
                     /locus_tag="PK112_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11297.1"
                     /translation="MKRMRVLWPAVIGLVCLALACSKNATDDGDNGGEDDLTAPAAIT
                     DLRALTVTTGSVVLGWTAPGDDGLTGIANAYDLRASADTITDATFVWAVPLADIVSPG
                     VAGQPQTVVVDDLLSDSIYYFAMKTIDEAGNASGLSNCCRVHIPAELVVAFEDTAFER
                     VVREHIHKPTGDIHSSDVDTIGELNANEQHIADITGVEYFTSLAFLHLLGNNITDLSP
                     VAGLPALVSLNAVGNDISDVSPLAGLTGLRDLALSQNPLATLAPLAGLGNLTQLSLDY
                     VSVADLSPLYGLEKLELLQTEDVAIACMAEAGNGEAVLCLGNGG"
     gene            complement(1108..1941)
                     /locus_tag="PK112_04275"
     CDS             complement(1108..1941)
                     /locus_tag="PK112_04275"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HPC11298.1"
                     /translation="MTLTTDRRMFLAACATALLLPETLGALVPQDSASADSTAGALDN
                     DRDLQKILRELPQAREHLPRLRAACARHAGIYPIPPVLAAKVLAIESGFADDAISSSF
                     AAGAAQLMHTTARELGAALPEADVFADQDEVLRLRGAFQRKMSEAVAAFQEGDDAGAR
                     AQRAEAETLREDYDLLHATTMESFRRRMFALTPEARRAYDARFDPAVADDLVVRYLAM
                     LARGVKRDLNLSDEAHVLLLAGVAYNAGPGSVKRATGIPVVAQNVEYANKLMAFQRLS
                     L"
     gene            2121..2678
                     /locus_tag="PK112_04280"
     CDS             2121..2678
                     /locus_tag="PK112_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11299.1"
                     /translation="MGAGKTVARSRLSEDLVRVFKSLVIWSFGAMLLAPFVSPRAARP
                     EPVPSDTAARLEPRSVTSGLFLAAVFPGPFIHGAGNYYAGAPAAGAVMTVAEIVGAGA
                     IIAGFASGGLDLFDNEEDDETGEALMAGGLVLFWGSWLADIATTPFQVHRYNRRLAQR
                     GMVFGVYAAPPAQGRPPAFGVTIRF"
BASE COUNT          458 a          955 c          949 g          482 t
ORIGIN      
        1 cgcccccgtt acccaaacaa agaacggctt ccccgttgcc tgcttcagcc atgcaggcga
       61 tggcaacatc ctcggtctgg agaagctcca gcttctccag accgtagagc ggcgagaggt
      121 cggcgaccga cacatagtcg aggctgagtt gcgtcaggtt tccgagtccg gccagcggcg
      181 ccagagtcgc caaagggttt tgcgagagcg cgagatcccg cagaccggtc agcccggcca
      241 gcgggctgac atcgctgatg tcgttgccga ctgcgttcag cgagaccagc gccggcagtc
      301 cggccaccgg cgacaagtcc gtgatgttgt tgccgaggag gtggagaaag gcgagcgagg
      361 tgaaatattc cacccccgtg atgtcggcga tgtgctgctc attggcgttc aattcgccga
      421 tcgtgtcgac gtcggaagag tgaatgtccc cggtcggctt atggatatgc tcgcggacga
      481 cccgctcgaa ggccgtatcc tcgaaagcga cgacaagctc ggccggaatg tgcacgcggc
      541 agcaattcga taggccgctc gcgttgccgg cctcatcgat cgtcttcatc gcgaagtagt
      601 agatgctgtc ggagagcaga tcgtccacca ccaccgtctg cggctgtccg gcgacgccgg
      661 gagatacgat gtcggccagc gggactgccc agacgaaagt ggcgtcggtg atggtgtcgg
      721 cggacgcgcg caggtcgtag gcattcgcaa tccccgtcag gccgtcgtcc cccggcgcgg
      781 tccacccgag cacgactgaa ccggtggtga ccgtcagcgc ccgcaggtcg gtgatcgccg
      841 ccggggcggt gaggtcgtcc tccccgccgt tgtcgccgtc atcggtggcg ttcttcgaac
      901 aggccagggc caggcagacc aacccgatga ccgccggcca cagtactctc atgcgcttca
      961 tgtccgacct cctgaatcaa aacctgcgcg ccctcggacc ggccggccgg ttgagtcaac
     1021 cgccggggat cctcgtggac catggcggta atgtaccgcg ccgtccctga cagtgtcaac
     1081 ggcgaactgg gggctcgatc gcgcgcctca cagcgacagc cgctggaagg ccatcagctt
     1141 gttcgcatac tcgacgttct gcgccaccac agggatgccg gtagcgcgct tgacgctgcc
     1201 gggcccggcg ttgtacgcga cgccggcgag caggagaacg tgggcttcgt cggagaggtt
     1261 gagatcccgc ttcacgccgc gggcgagcat ggcgaggtag cgcacgacca ggtcgtcggc
     1321 caccgccggg tcgaaccggg cgtcgtaggc ccggcgcgcc tccggcgtga gggcgaacat
     1381 gcgccgccgg aagctctcca tcgtggtggc gtggagcaga tcgtagtcct ctctgagggt
     1441 ctccgcctcg gcccgctggg cccgcgcccc cgcgtcgtcg ccttcctgaa aagccgccac
     1501 cgcctcgctc atctttcgct ggaacgctcc gcggagccgc aagacctcgt cctgatcggc
     1561 gaacacgtcg gcttcgggga gcgcggcgcc caactctcgc gcggtggtgt gcatgagttg
     1621 cgccgcaccc gccgcaaagg aggacgagat agcgtcgtcg gcaaagcccg attcgatggc
     1681 cagcaccttg gcggccagca ccggcggaat cgggtaaatc cccgcgtgtc tggcacaggc
     1741 cgcccggagg cggggaagat gttcgcgcgc ctgcggcagc tcccggagga tcttctgcag
     1801 gtcccggtcg ttatccagcg cgcccgccgt cgaatcggcg ctcgccgagt cctgcgggac
     1861 cagcgcccca agcgtctccg gaagcagcag cgcggtcgcg caggcagcaa ggaacatccg
     1921 acggtcggtt gtcaaggtca tgatctccgt tctcttccgc cgccgagtca gcctcggcgg
     1981 ccgccgttca gtgtggctgc gttcttccga tgttacgtcc gggacggcaa ttgtcgccat
     2041 aaaacagacc ggcgccggat ttttgtgaca gtttttgtcg ccgcgcggcc ggattttgcg
     2101 tataatcggt cggcggacac atgggcgctg ggaaaaccgt cgcccgctcg cggctgtcgg
     2161 aggatctcgt gagagtattc aaatcgctcg ttatctggtc gtttggagca atgctcctgg
     2221 cgccgttcgt ttcgccccgg gccgcccggc ccgagccggt tccctccgac accgcggccc
     2281 gcctggaacc tcggtcggtg accagcgggc ttttcctggc cgccgtattt ccgggcccct
     2341 tcatacacgg cgccggcaac tattacgcgg gcgccccggc ggccggcgcg gtcatgacgg
     2401 tcgcggaaat cgtcggcgcc ggggcgatca tagccggctt cgcctcgggc ggcctggact
     2461 tgttcgataa cgaggaggac gatgagaccg gcgaggcgct catggccggc ggcctcgtgc
     2521 ttttctgggg ctcctggctt gccgacatcg ccaccacccc cttccaggtg caccgctaca
     2581 accgccgcct tgcccagcgc ggaatggtgt tcggggttta cgcggcgcct cccgcacagg
     2641 gccgcccgcc cgctttcggg gtcacaatcc gattctgagt aagcgccctg cttcaggaat
     2701 gcggaactct ccccggtggg agcgtcaccg cccgaggatg cgggcgggaa gcgtcagcac
     2761 cgcccggagc agctccagca cgccgtcgac cgccgccccg accacgcgga acggcagcag
     2821 gaggagccag acgatcgggt agag
//
LOCUS       DAOOKS010000209         2842 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_43133_length_2842_cov_3.213491, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000209 DAOOKS010000000
VERSION     DAOOKS010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43133_length_2842_cov_3.213491"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="PK112_04285"
     CDS             <1..666
                     /locus_tag="PK112_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11300.1"
                     /translation="FQPLSDGSVYLSFLDSTLWYRIIPALLGVMMWTRFSKNYQWVSR
                     WPMSMYIGIAVGVSIPLDLGNRVIRQLQATMYDFKWDTWGDFIGSSNLFYVLDPFSGL
                     SQVIIAVGSIAGLTYFFFSKAHTGVFGRVATFGIWILMIGFGASFGFTVMARISLFIN
                     RVQDVDSWRKVGWNAGETPIEGYSAWYQLVGWLLVAVVVAYIVNEIRLYLKSRNQVAG
                     PQV"
     gene            1333..1545
                     /locus_tag="PK112_04290"
     CDS             1333..1545
                     /locus_tag="PK112_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11301.1"
                     /translation="MHKEKAKEYQRQYNLKHKKKARGGRGKANSLCPREVVRTTFNSS
                     DIMHAPAEKAARILDKIIKGERLFTM"
     gene            1980..>2842
                     /locus_tag="PK112_04295"
     CDS             1980..>2842
                     /locus_tag="PK112_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HPC11302.1"
                     /translation="MKALLGGKGANLAEMSRAGVPVPPGFTITTEVCNLYYDNNLQVP
                     GPIDADLEKHVGMIEKSVGKEFGNPADPLLVSVRSGAKFSMPGMMDTVLNLGLNAETV
                     EGLAKKTGSQRFALDVYRRFIAMFGNVVLGIDKSEFEQVIDKKKKERRIKQDSSLQEG
                     DLRDIIKKFKHICRRKSGEEFPEDPWQQLRMARDAVFRSWNNPRAISYRRMHDIPSDL
                     GTAVNIQAMVFGNMGNNSATGVGFTRNPATGTKEFYGEYLVNAQGEDVVAGIRTPQPI
                     VQLKEEMPQAY"
BASE COUNT          644 a          764 c          893 g          541 t
ORIGIN      
        1 ttccagccgc tgtcggacgg cagcgtgtac ctctcttttc tcgactccac cctatggtac
       61 cgcatcatcc cggccctcct cggcgtgatg atgtggacgc gtttctcgaa gaactatcag
      121 tgggtgtcgc gctggccgat gtcgatgtac atcggtattg cggtcggcgt ctccattccg
      181 ctcgatctcg gaaaccgggt gatccgccag cttcaggcga ccatgtacga cttcaagtgg
      241 gacacctggg gcgacttcat cggcagctcc aacctgttct acgtgctgga tccgttcagc
      301 gggctgtcgc aggtgatcat cgcagtcgga tcgattgcgg ggctgaccta cttcttcttt
      361 tcgaaagccc acaccggggt gttcgggcgg gtcgcgacct tcggcatctg gattctgatg
      421 atcgggttcg gcgcctcgtt cggattcacc gtgatggcgc gtatttcgct gttcattaac
      481 cgcgtgcagg atgtcgacag ttggcgcaaa gtcggctgga acgcggggga aacacccatc
      541 gaggggtact cggcctggta ccagctggtg ggttggctgc tggtggcggt cgtggtggcc
      601 tatatcgtaa atgagatacg gctgtatttg aagagccgga atcaggtcgc cgggccgcag
      661 gtttgagcgg accgaccggg tgacgattaa tcccgccggg tctataaccg gcgggatttc
      721 ttttttcttt gagggttgca atttttcgat agttttttat tgatttgagt ttatggaatt
      781 atactgttgg ggctgaagct attggtctgc atggaacttt tcggggttgt ttaacgcatc
      841 gcatgaccga cagacggagt ttgaggcagg cgacaatgac caggcagtgt atgttgaaaa
      901 ttccggacgc tgcagttagc gtactgtcgc acaggggctt ccgccgggtc gcgacaatac
      961 gcgtaacagg cgccgggaaa ggaggttgta gaagaagaga aaacgggatt tgtcaagtca
     1021 ttatgtaaat gaagattacc gaaaaatatt tcgcgtcttc ggaaaaaaat gcttgacata
     1081 cagtaaggat ttcctatcat ccctgtggag gagccaagcg cgaccggagt ggagcccgga
     1141 agcgcatcgt ccgttacgaa aagggtagcc actacaacac agggaaagtg aggtaggaac
     1201 gtggctcggc ctcgcataac gactgtagcc aatcccgtcg cgcattcgac taccgctccc
     1261 gagaacgggg agaccagaac aaagctttcc acggccgagg cgcgccgaga gtatcagcga
     1321 cggtactacc agatgcacaa ggagaaggcg aaggagtatc agcgccagta caatctgaag
     1381 cacaagaaga aagcgcgcgg cggtcgcggg aaagccaatt ctctctgccc gcgcgaggtg
     1441 gtgcgcacga ctttcaacag ctcggacatc atgcatgcgc ccgccgagaa agcggcgcgc
     1501 attctcgaca agatcatcaa gggcgaacgt ctcttcacga tgtagcgccg cgatggcgct
     1561 ctcgacaggt ttcaggcggc cggaactcac gttaagtttc ggccgccttt tgtgttgcgc
     1621 cgatggagat ttttgtatag attcgcacaa attttcacaa cgcgattcgt tggcgaaagg
     1681 gacattctat gccagctaag aaggcggcca agaagacggt gaagcagacg gccaaacaag
     1741 cctccaagaa ggatgcgggt cggaagacgg cgcggccgac tccgagcgag aaaacgccga
     1801 aaaagatcgc cccgccgaaa gatgcgggcg gtctgaaaaa aacggcgcac aaaccggcgc
     1861 cggcggccaa aagaccggcc ggaaagaacg tcgccgtgtc ggccaaaatc gccgaggccg
     1921 tggcgaaggg gaaattctac tactacttcg gggacggcaa ggcggacggc agcgccgaca
     1981 tgaaggcttt gctggggggc aaaggcgcaa acctggccga gatgagccgg gccggagtgc
     2041 ccgtgccgcc cgggttcacg atcaccaccg aggtgtgcaa cctctactac gataacaacc
     2101 tgcaggtgcc cggcccgatc gacgccgacc tcgagaaaca cgtcggcatg atcgaaaaat
     2161 cggtcggcaa ggaattcggc aacccggccg atccgctcct ggtctcggtg cgctcggggg
     2221 cgaagttctc catgccgggg atgatggata ccgtcctcaa cctcggactc aacgccgaga
     2281 cagtcgaagg tctggcgaaa aaaacgggca gccagcggtt cgcgctcgac gtttaccggc
     2341 ggttcatcgc gatgttcggc aacgtcgtcc tgggcatcga caagagcgag ttcgagcagg
     2401 tgatcgacaa gaagaagaaa gagcgccgga tcaagcagga ttcatcgctg caggaagggg
     2461 acctgcggga cattatcaag aagttcaaac acatctgccg gcggaaatcc ggcgaggagt
     2521 tccccgagga tccgtggcag caactgcgca tggcgcgcga cgccgtgttc cgcagctgga
     2581 acaacccgcg cgcgatctct taccggcgga tgcacgatat cccgtcggat ctcggcacgg
     2641 ccgtgaacat tcaggccatg gtgttcggga acatggggaa caactctgcg accggggtgg
     2701 ggttcacgcg caatcccgcc accgggacga aggagttcta cggcgagtat ctcgtgaacg
     2761 cgcagggaga ggacgtggtg gccggcatcc gcacgcccca gccgatcgtc cagctcaagg
     2821 aggagatgcc gcaggcctac aa
//
LOCUS       DAOOKS010000210         2842 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_43135_length_2842_cov_2.933979, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000210 DAOOKS010000000
VERSION     DAOOKS010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43135_length_2842_cov_2.933979"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..102)
                     /locus_tag="PK112_04300"
     CDS             complement(<1..102)
                     /locus_tag="PK112_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019468450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HPC11303.1"
                     /translation="MDISAQLVKELREKTGAGMMDCKKALTEADGDFD"
     gene            complement(116..904)
                     /gene="rpsB"
                     /locus_tag="PK112_04305"
     CDS             complement(116..904)
                     /gene="rpsB"
                     /locus_tag="PK112_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009080421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HPC11304.1"
                     /translation="MVSPQVKDLLEAGVHFGHQTRRWNPKMKPFIFAARNGIYIIDLQ
                     KTVNALEQARRKMAETVGKGKSVLFIGTKKQAQDVIKEESARCGGYYVTERWLGGMLT
                     NFQTIKYSVKKLKDIEKMRVDGTLEKFSKKERSRIEHEAGKLQKYLGGIKEMGHLPGL
                     VVIVDARKERIAVAEAHKLGIPIIGIVDTNADPDLIDFPIAGNDDAIKSIRILMHALV
                     DAAVEAKSTVVTEMLAASEREGAPGNGHSPAAGRHDVANKDQAE"
     gene            complement(1047..1439)
                     /gene="rpsI"
                     /locus_tag="PK112_04310"
     CDS             complement(1047..1439)
                     /gene="rpsI"
                     /locus_tag="PK112_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013933201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HPC11305.1"
                     /translation="MEKTEYAATGRRKCSVARAVIANGDGGFTINGSDIAAYLKRDPL
                     VVHATEALTITEMSGKVAVVCRASGGGLSGQAGAVRLAVARALARLNEDFRPTLRQNG
                     LLTRDPREVERKKYGRPKARKRFQYSKR"
     gene            complement(1456..1887)
                     /gene="rplM"
                     /locus_tag="PK112_04315"
     CDS             complement(1456..1887)
                     /gene="rplM"
                     /locus_tag="PK112_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005381375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HPC11306.1"
                     /translation="MKTFIPKVDPNNRKWWVVDVEGHILGRAAVQVANILRGKNKPIF
                     TPHLDAGDHVVVVNARSIRVTGKNKPRQKTYYRFSGYPGGLRSTSLSTMLRQKPEETF
                     RLAVRRMLPKNRLGRKMIKKLHVYADGEHRHQAQKPEPYKL"
     gene            complement(2080..>2842)
                     /locus_tag="PK112_04320"
     CDS             complement(2080..>2842)
                     /locus_tag="PK112_04320"
                     /inference="COORDINATES: protein motif:HMM:NF021759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="HPC11307.1"
                     /translation="RGDPPVNPDTSAARGRAFNVEIRNADSLYITDTQVKARVDSKRQ
                     KTAMRACNYELFQFETLAPPPPATVDTKIRQATQAARPEVAARTDGAAARPTSELPGL
                     DELYRLFDYYNWKYFGGRLPRVKIEYSTRMTCAGSYSPNRRLIKIGRRYHEIFPQDLE
                     DTLKHEMIHIRHYHHDAKFKAEAVRIGASLKAREHPNLRRPPKYVYECPGCGAEYPRQ
                     RRLVQASCTYCSANRRYDPRFKLRRKRPRVPRSGT"
BASE COUNT          437 a          926 c          844 g          635 t
ORIGIN      
        1 gtcgaagtca ccatcggcct cggtcagggc cttcttgcag tccatcatgc cggccccggt
       61 tttttcccga agctctttca ccagttgtgc gcttatgtcc atgtgtcgaa ccttcttact
      121 cagcttggtc tttattcgcg acgtcgtgtc ggccggccgc cggggagtgg ccattacccg
      181 gcgctccttc acgctccgac gccgcgagca tctcggtcac caccgtgctc ttggcctcca
      241 ccgccgcgtc gaccagcgcg tgcatcagga tccggatcga cttgatcgcg tcgtcgttgc
      301 cggcgatcgg gaaatcgatc aggtcgggat cggcgttggt gtcgacgatg ccgatgatgg
      361 ggatccccag cttgtgggcc tcggccaccg cgattcgctc cttgcgggcg tcgacgatca
      421 cgaccagccc cgggaggtgg cccatctcct tgatcccgcc gaggtacttc tggagctttc
      481 ccgcctcgtg ctcgatgcgg gagcgttctt tcttggagaa cttctcgagc gtcccgtcca
      541 cccgcatctt ctcaatatct ttgagttttt tgaccgagta cttgatcgtc tggaaattgg
      601 tcagcatgcc gccgagccag cgttcggtga cgtagtagcc gccgcagcgg gcggactctt
      661 ccttgatcac gtcctgggcc tgttttttgg ttccgatgaa gaggacggat ttccccttgc
      721 ccaccgtttc cgccattttg cggcgggcct gttccagggc gttgacggtt ttctgcaggt
      781 cgatgatgta gatgccgttg cgggcggcga agatgaacgg cttcattttg gggttccacc
      841 ggcgcgtctg atggccaaaa tggaccccgg cctcgagcaa atcctttacc tggggactaa
      901 ccatggaatg ccttcgtgtt gagcggttag gtctttaccc cgacatcgcc tcgacccgga
      961 cccgcggatt cggggaccgc cggatcgatc cgtcgggatt gtatattttc ccgccgaggc
     1021 gggaatggca actcttcgta cccggcttag cgcttcgagt actggaaccg tttgcgggct
     1081 ttcgggcggc cgtacttctt gcgctccacc tcgcgggggt cgcgcgtgag caggccgttc
     1141 tggcgcagcg tcggccggaa atcttcattc agacgggcca gcgcccgggc caccgccagc
     1201 cgcaccgccc ccgcctggcc gctcagtcca cccccgctgg cccggcacac gaccgccacc
     1261 ttcccggaca tctcggtgat cgtgagggcc tcggtggcgt ggaccaccag cggatcgcgt
     1321 ttcagatagg cggcgatgtc ggacccgttg atcgtgaaac cgccgtctcc gttggcgatc
     1381 acggcgcggg cgacactgca tttgcgacgt cccgtcgcgg cgtactcagt cttctccatg
     1441 ggcaaacgtc aaactctaca gcttataagg ttccggtttc tgcgcctggt ggcggtgctc
     1501 gccatcggcg tacacgtgca gtttcttgat catcttccgg cccagccggt ttttcggcag
     1561 catccgccgc accgccagcc ggaacgtctc ctcgggtttt tgccgaagca tggtggagag
     1621 actcgtgctc cgcagtccgc ccggatagcc cgaaaaccgg tagtacgtct tctgccgggg
     1681 cttgtttttc ccggtcaccc ggatgctgcg ggcgttgacc acgacgacgt ggtcgccggc
     1741 gtccaggtgg ggcgtgaaga tgggcttgtt cttgccgcgc agtatgttcg ccacctgcac
     1801 cgccgcccgt ccgaggatgt gcccttcgac atccaccacc caccacttcc ggttgttcgg
     1861 gtcaactttc ggaataaacg tcttcatctc tatattctca taacctcata gttttcaccg
     1921 tcaagacagc ctgcaagtat actgcattcc gcgtcaatgt caatacggtt cagcaaaata
     1981 attggcctgg ccttcggacc gccgcctccc cggggcagtt ccactgggcg agacgcaact
     2041 tgttgggaat caaccgatag caggaccgct cccagcccct cacgtccccg agcgcggcac
     2101 ccgaggcctt ttccgccgga gcttgaaccg gggatcgtag cgccggttgg ccgagcagta
     2161 ggtgcacgag gcctgcacca gcctccgctg acgggggtac tccgccccgc acccgggaca
     2221 ctcgtagaca tacttcgggg gccgccggag attcgggtgt tcccgcgctt tcagcgaggc
     2281 gccgatccgg accgcctccg ccttgaattt cgcgtcgtgg tggtagtggc ggatatggat
     2341 catctcgtgc ttgagcgtat cttcgaggtc ctgaggaaaa atctcgtggt accgccgccc
     2401 gatcttgatc agccgtcggt ttggtgaata cgatccggca caggtcatcc gcgtcgaata
     2461 ctcgattttc acccgcggca acctcccccc gaaatacttc cagttgtagt agtcgaacag
     2521 ccgatagagt tcatcgagcc ccggcagctc gctggtcggc ctggcggccg cgccgtcggt
     2581 ccgcgccgcc acctcggggc gcgcggcctg ggtcgcctgc cggattttcg tatcgaccgt
     2641 agccggcggc gggggggcga gcgtctcaaa ctgaaacagc tcgtagttgc acgcgcgcat
     2701 cgccgttttt tgccgcttgc tgtccactct cgccttcacc tgtgtatccg tgatgtataa
     2761 gctatcggca ttccgaatct caacattaaa cgcccggccg cgggcggcgc tcgtgtccgg
     2821 gttcacggga ggatcacccc gg
//
LOCUS       DAOOKS010000211         2841 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_43160_length_2841_cov_2.479182, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000211 DAOOKS010000000
VERSION     DAOOKS010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43160_length_2841_cov_2.479182"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1218)
                     /locus_tag="PK112_04325"
     CDS             complement(<1..1218)
                     /locus_tag="PK112_04325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPC11308.1"
                     /translation="MVRFTVQLFGVTVFALGLIWFVGSGEAQQSDPKEPVLWADTFHI
                     NLEETPDSAALESRRQDRDSFLMDVKKLTHTALNIRNQYMEEVNTEEIIKAGINGMLS
                     DLDRFSVLMEKSSYDALMESTHGKYEGLGMQIDERNDRIVIISPIEGTPAYNKGLRAG
                     DIIWAIDGKSTEGMTSAEASKMMRGKAGTSIKLAIKRNGIEEPIEFELERALIELKSV
                     NYAGVIPGTTIGYVRLSRFAEETSHELRQAISDLNGKGITALVFDLRSNGGGLLDQAK
                     ETAELFLQEGREIVYTKGRFENTERHYRAERPPLFPPDKPLVILVDEGTASASEIVAG
                     AVQDWDRGLIVGNTTYGKGLVQQIFPISSDGSMALKLTTAKYYVPSGRCIQKPETQAK
                     PGSHAALEDEETEA"
     gene            1561..2352
                     /locus_tag="PK112_04330"
     CDS             1561..2352
                     /locus_tag="PK112_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11309.1"
                     /translation="MTGNRPKFRNSPAAALLLHLLAPGFGHFYWREYLFGVFIFLVLL
                     MAGALFVLSLLIDLPFYGRLIIYGLPIIFYLISFGDLYRIARREHTARPQTSRRALVI
                     WLLAAAYELVWPLAPLNFALLNAPSVYVMRENNLAPVFRDGDLLKASRLAYTFHTWFV
                     NKPIVHALPDRFAVVRVTAEGRALTGIVLGLPGEEVEVVDGVVVVDGTPQVVPTTASR
                     LLNGDYPLTQAGGYSLLIALPEYGRIARVYEIPLGDLIGKVEKVL"
     gene            2600..>2841
                     /locus_tag="PK112_04335"
     CDS             2600..>2841
                     /locus_tag="PK112_04335"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HPC11310.1"
                     /translation="MSRIKNLIFDLDGTLIDSSEGVVAAFHYAFAAAGLPLPPEEVKR
                     FIGYPLETMFAHFTDYSPERLRTYFRERAAETIVAST"
BASE COUNT          478 a          937 c          814 g          612 t
ORIGIN      
        1 cgcctcggtt tcctcgtcct cgagggccgc gtgcgacccc ggcttggcct gtgtctccgg
       61 cttctggatg caccgtcccg agggcacgta gtacttggcc gtcgtgagct tgagcgccat
      121 gctcccatcc gacgagatcg ggaaaatctg ctgcacgagc cccttgccgt aggtcgtgtt
      181 gcccacgatg aggccgcggt cccagtcctg cacggcgccg gcgacaattt ccgaggctga
      241 ggccgtgcct tcatcgacca ggatcaccag cggcttgtcc ggcgggaaca gcggcgggcg
      301 ctccgcgcga tagtggcgct cggtgttctc aaaacgccct ttggtgtaga cgatctcgcg
      361 cccctcctgc aagaacaact cggccgtctc cttagcctga tcgagcagtc ccccgccgtt
      421 ggagcgcagg tcgaacacca gcgccgtgat ccccttgccg ttgagatcgc tgatcgcctg
      481 gcgcagttcg tgcgaggtct cctcggcgaa ccgcgagagc cgcacgtacc cgatcgtcgt
      541 ccccggaatc accccggcat agttcaccga cttcagttcg atcagcgctc gttcgagctc
      601 aaactcgatc ggctcctcga tcccgttgcg cttgatcgcc agcttgatcg acgtcccggc
      661 tttgccgcgc atcattttcg acgcctcggc cgaggtcatc ccttccgtcg acttcccgtc
      721 gatcgcccag atgatatccc ccgcccggag acccttgttg taggcgggcg tcccctcgat
      781 cggggagatg atgacgatgc ggtcgttgcg ctcgtcgatc tgcatcccca gcccctcgta
      841 cttcccgtgc gtcgactcca tcagggcgtc gtacgagctc ttctccatga gcacgctgaa
      901 gcggtcgaga tccgagagca tcccgttgat gccggccttg atgatctcct cggtgttgac
      961 ctcctccatg tactgattgc ggatattgag cgccgtatgg gtgagcttct tcacgtccat
     1021 caggaaggag tcgcggtcct ggcggcggct ctcgagggcg gccgagtcgg gcgtctcttc
     1081 caggttgatg tggaaggtat ccgcccagag caccggttct ttggggtcgg actgctgggc
     1141 ctcgccggag cccacgaacc agatcaagcc gagcgcgaat acggtgacgc cgaagagctg
     1201 aactgtgaag cgaaccatac ggcctccaga ctcattgaga gtgacgaaca caatgtatcg
     1261 taaatcggag acttgtcaag tttgcggact ttttccgaag tacgctgtat ctgcctgcaa
     1321 attctacgct taagccggcg cagcggttgc accgaaactg accaccggcg gatggcgcat
     1381 aaataacttc ttgcctgccg actgcttagc acgttaatac cactgtatcg gtcatcggca
     1441 accgttcccg gaatcggggc ggcagtcgcg cgggcaacgc aacacgtttc ccgataaggt
     1501 attagctgca agactggacg ttgtaccggc aacaacattg ttgcctttct gaacagtaga
     1561 atgacgggca atcgaccaaa gttccgcaac tcccccgccg cagcccttct tctgcatttg
     1621 ctggctccgg ggttcggcca tttttactgg cgggagtacc tcttcggcgt cttcattttc
     1681 ctggtcctcc tcatggccgg agcgctgttc gtcctctccc ttctgatcga tctcccgttc
     1741 tatggccggc tgatcatata cggccttccg atcattttct atctgatctc tttcggcgac
     1801 ctctaccgga tcgcgcggcg cgagcatacc gcccgtccgc agacgagccg ccgggcgctg
     1861 gtgatctggt tgctggccgc ggcctatgaa ctggtctggc cgctggcccc gctgaatttc
     1921 gccctcctga atgctccgtc ggtctatgtc atgcgcgaga acaacctcgc cccggtattc
     1981 cgcgatggcg acttgctgaa agcttcccgc ctcgcctaca cctttcacac ctggtttgtc
     2041 aacaaaccga tagttcacgc tctaccggac cggttcgcgg tggtccgggt cacggccgag
     2101 ggccgggcgc tcacggggat cgtcctcggc ctccccggcg aggaggtcga agttgtcgac
     2161 ggggtggtgg tggtcgacgg cactccgcag gtcgttccga ctaccgcgag ccgcctgctc
     2221 aacggcgact atccgctcac acaagccggc ggctactcac tcctgatcgc gctccccgaa
     2281 tacggccgca tcgcccgggt ttatgagatt cccctgggcg acctgatcgg caaggtggag
     2341 aaagtattgt gaacgccacg gccgccatgt ggcgggagca cagcgcgaga tgcgctcgag
     2401 gtctggatct tcgtgggccg gacgcttttc ttccgttccg caaacacgcc cgtcaggacc
     2461 atgaaggccc tgagctacag aaatccgcga tgactcagtg ccctcttgta ggtcaggacc
     2521 cttgtggtcc tgacgctctt cttccgttcc gcaaacactt ccggcgtgac cgccactatc
     2581 cggaggcggg ctactgccga tgagccggat caagaacctc attttcgacc tcgacggcac
     2641 cctgatcgac tcctccgagg gcgtcgtggc ggcattccac tacgccttcg ctgccgccgg
     2701 ccttccgctt ccgcccgaag aagtcaagcg gtttattggg tatccgctcg agacaatgtt
     2761 cgcgcacttc accgactact cccccgaacg cctgaggacg tacttccgcg agagagccgc
     2821 cgagacgatt gtcgcgtcga c
//
LOCUS       DAOOKS010000212         2832 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_43353_length_2832_cov_3.192654, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000212 DAOOKS010000000
VERSION     DAOOKS010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43353_length_2832_cov_3.192654"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /gene="amrB"
                     /locus_tag="PK112_04340"
     CDS             complement(<1..203)
                     /gene="amrB"
                     /locus_tag="PK112_04340"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HPC11311.1"
                     /translation="METDRATIRKPAVAGAFYPRDPGELATAIARLYAQAGKRIITGH
                     PVVVVAPHAGYPYSGLTAAKAYK"
     gene            complement(274..1248)
                     /locus_tag="PK112_04345"
     CDS             complement(274..1248)
                     /locus_tag="PK112_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HPC11312.1"
                     /translation="MDTGILIFLLAAIAFAVVIVSFSVKVVGPYERGVVVRLGKYQRV
                     LAPGLNVIFPLLDDVRKIDMREQVLDVPPQQVITKDNVVVEVDAVIWAQVTDPVRATF
                     EISNYFDAASKLAQTNLRNVMGEMDLDTCLSSRDKINGQLRDVLDDATDKWGVRINRV
                     ELQRIDPPRDITEAMSRQMKAERDKRAAILEAEARRQAAILLAEGNGKAAVLEAEGFA
                     EAVRMKADAERYRQITVAEGEALAINNVFGAIHASRPDDKLIMLKYLEMLPKLAENQA
                     NKIFLPYEASGMIGALAALIEGARPNGGGKTASPRPAIAPVDTEEVAG"
     gene            complement(1254..1694)
                     /locus_tag="PK112_04350"
     CDS             complement(1254..1694)
                     /locus_tag="PK112_04350"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HPC11313.1"
                     /translation="MATMFWIWMAAAVIFLIIEITSPSFLAICFAIAAAAAGVFAQFN
                     PESYYWQVGLFIIASLVLLPLMRPLARRITKPSPVQSNIDRLIGQIGIVTKPIDRESP
                     GQIVVDGETWRAVAAGTFAPAERVRVASVQGNRLRVERAEESEE"
     gene            2093..2443
                     /locus_tag="PK112_04355"
     CDS             2093..2443
                     /locus_tag="PK112_04355"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPC11314.1"
                     /translation="MTNVARRETNGMRNRLPSPFFEDFFHWPPAPWEPHHWPRVDVRE
                     TSEDATLLFEAPGMRKEDLEVQVGNNRLTVSGERRLEADDSYEGWGPPRDRDRDIQAP
                     VYAPVGYRPALGQG"
     gene            complement(2594..>2832)
                     /locus_tag="PK112_04360"
     CDS             complement(2594..>2832)
                     /locus_tag="PK112_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11315.1"
                     /translation="FFAGFLFGLPIVLHIAQSAVARIRARTGDVVLLLDSGVIAFLLV
                     MPFSYLAWEKYMMPLAPLVLARLAALRVPGDPAS"
BASE COUNT          501 a          863 c          993 g          475 t
ORIGIN      
        1 tgcttgtagg ctttggccgc agtcaggccc gagtacgggt agccggcgtg gggggcgacg
       61 accactaccg ggtggccggt gattatgcgt ttgcccgcct gggcatagag tcgggcgatg
      121 gcggtggcca gctcgcccgg gtcgcgcggg tagaaggcgc ccgcgacggc gggcttgcgt
      181 atggtcgcac ggtcggtttc catggcgata gtatacgccg gggaaggcgg gtgtgtcaaa
      241 tggagggggg cgagccggtc caattaaatc ggctcagccg gcgacctctt cggtatcgac
      301 cggggcgatc gcgggccggg gcgacgccgt tttgccgccg ccgttgggcc gcgccccttc
      361 gatgagcgcc gccagcgccc caatcatccc gctcgcttcg tacggcagga agatcttgtt
      421 cgcctggttc tcggccagtt tcggcagcat ctcgaggtat ttcagcatga tcagcttgtc
      481 gtcgggccgg ctggcgtgga tggcgccgaa gacgttattg atggcgagcg cctctccctc
      541 ggccaccgtg atctggcggt agcgctcggc atcggctttc atacggacgg cctcggcgaa
      601 tccctcggct tcgaggacgg cggccttgcc gttgccctcg gccagcagga tggctgcctg
      661 gcgccgggcc tcggcctcga ggatagcggc ccgcttgtcg cgctccgctt tcatctggcg
      721 gctcatcgct tcggtgatgt cgcggggggg gtcgatgcgc tggagttcga cgcggttgat
      781 gcggacgccc cacttgtcgg tggcgtcgtc gaggacatcg cgcaactggc cgttgatctt
      841 gtcgcgggac gagaggcagg tatcgaggtc catctccccc atcacgttgc gcaggttggt
      901 ctgggccagc ttggaggcgg cgtcgaagta gttggagatt tcgaaagtcg cccgcaccgg
      961 atcggtcacc tgcgcccaga tgacggcgtc gacctcgacc acgacgttgt ccttggtaat
     1021 gacctgctgc gggggaacat cgagcacctg ctcgcgcatg tcgatcttcc ggacatcgtc
     1081 gaggagcggg aaaatgacgt tgaggccggg cgcgaggacg cgttggtatt tgccgaggcg
     1141 cacgaccacg ccccgttcgt agggaccgac cactttgacc gagaacgaga cgatgaccac
     1201 ggcgaaggcg atcgccgcga gcagaaatat caggattccg gtatccatgg ggctcactcc
     1261 tcactttcct cggctctttc aacgcgcagg cggttcccct ggacggaggc gacccgcacg
     1321 cgctcggcgg gggcgaaggt tccggcggcg acggcccgcc acgtctcgcc gtcgaccacg
     1381 atctgcccgg ggctctcgcg atcgatcggt ttggtcacga tgccgatctg tccgatcagg
     1441 cggtcgatgt tggattgcac cggcgagggt ttggtgatgc gccgcgccag ggggcgcatc
     1501 agcggcagga gcacgagcga ggcgatgatg aacaacccca cctgccagta gtagctttcg
     1561 ggattgaact gggcgaagac gccggccgcg gccgccgcga tggcgaagca gatcgcgaga
     1621 aacgacgggc tggtgatttc gatgatcaga aaaatgactg ccgcggccat ccagatccag
     1681 aacatggtcg ccatagaagc tcctttgcgc gagtgtcggc gcggccgctg tcgcccggcc
     1741 gcaactggtc aaagctagcc acgaggcggc ccgggattca acaaaaactc cggttcgccg
     1801 gtcgccccgg agggcgtaaa cgcagccggc gggagccgcc gtcgcgcgcc ccggcccggc
     1861 gggaccgggg gggtacgctc gctctctgcc ggattgaccg catcgggaag cagaatcgac
     1921 agccgcagtt gccacgatgg gagacctgcg gcaggccgct ctcccaccgc gagagcaggt
     1981 gcttggccga gcgcgtgcga ccgaatgggt acatgctttg attacgtgca gagtgaaaac
     2041 aacaacagcc atagatgacc tagctgaggc aaacgcaagg aggtgttgcg aaatgacgaa
     2101 cgtggcgaga cgtgaaacta acggcatgcg gaatcgcctg cccagcccgt tcttcgagga
     2161 cttcttccac tggccgccgg cgccgtggga gccgcatcac tggccgcggg tcgatgtccg
     2221 ggagacgagc gaggacgcca ccctcctgtt cgaggccccg ggcatgcgca aggaggatct
     2281 cgaagtgcag gtcgggaaca accgcttgac ggtgtcgggc gagcggcgcc tcgaggccga
     2341 tgactcctat gaggggtggg gtccgccgcg agatcgcgac cgggacattc aagcgccagt
     2401 atacgctccc gtcggctatc gaccagcact cggtcaaggc tgagtacaag aacggcttcc
     2461 tgcggctgac gcaggccaag cgggaggaag cgaagccgaa gcagattgac gtgaaagtcc
     2521 cgtgacgatg cggtctcctc gctctgcact gtgtgacccg gccggcccga tgggccggcc
     2581 gcgtgttttc gcgtcaggac gcagggtctc ccgggacgcg cagagcggcg agacgcgcca
     2641 gaaccagcgg cgccagcggc atcatgtatt tctcccacgc cagatacgaa aagggcatga
     2701 cgagcaggaa agcgatcacg ccgctgtcga gcaggagtac gacatcaccc gtccgggcgc
     2761 ggatccgggc gactgcgctc tgggcgatgt gcagcacgat cggcaacccg aagaggaatc
     2821 cggcaaaaaa ga
//
LOCUS       DAOOKS010000213         2829 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_43426_length_2829_cov_3.262437, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000213 DAOOKS010000000
VERSION     DAOOKS010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43426_length_2829_cov_3.262437"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="PK112_04365"
     CDS             complement(<1..220)
                     /locus_tag="PK112_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11316.1"
                     /translation="MPKRFLIFVAIVQGILWIGHALLGATAVAFFSIAPGPAAAILFV
                     LSLTFVSASLIGHRRHGPVVRLYYRFAAV"
     gene            complement(310..1260)
                     /locus_tag="PK112_04370"
     CDS             complement(310..1260)
                     /locus_tag="PK112_04370"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPC11317.1"
                     /translation="MIPSVADLYKIIEQSDLVYVLEHLDSVQVDSIDKARRLNSNMLY
                     LYVDSSSRSLVRHEIDDSLGVIFAIAEGAYEAGDFEAALAAYSEALLLRPSFDPAMVY
                     AGDAYFGMNELDSARAWYQRAVDANFINYQAHWFLGHMMWLQGDSARAIEELTIAHLL
                     NVNHMEIKKVLKSYREDAGGTWRGPDISLHYALTRDTSGVRVRFEAESVAYALVRAVW
                     KYEPGYAESRQGEDWRGSLMQEIAEAAVTWLDFMAGLDDTLAPAWWPQVEELAQEADD
                     SGQVEHFIWYAFIAPELPAAMMLMPREQFDAIREFVDKFY"
     gene            complement(1407..1478)
                     /locus_tag="PK112_04375"
     tRNA            complement(1407..1478)
                     /locus_tag="PK112_04375"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1443..1445),aa:Arg,seq:ccg)
     gene            1675..>2829
                     /gene="proS"
                     /locus_tag="PK112_04380"
     CDS             1675..>2829
                     /gene="proS"
                     /locus_tag="PK112_04380"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HPC11318.1"
                     /translation="MRWTHSYIPTLRENQAEAELISHQLLLRGGYIRKLASGIYLYLP
                     LMQRVIHKFSNIVREEMNRAGGLEIAMSVLCPAEVWQESGRYGTIGKEQMRLRDRHDH
                     EQVLCGTHEETVTFLIRGEVKSYRQLPLNLYQIQVKFRDEIRPRFGLMRGREFIMKDA
                     YSFDADEESFARSYQAMVDAYFRIFHRAGLDVVKVESDTGAMGGKAAHEFMLLVDTEA
                     GEEIIMSCDSCDYTANLEKAAFCDPLQPAPEPETKPRETVDTPGAATIEEVTAFLKVP
                     AHRLVKTLLYMADGKPVAALVRGDRELNETKLKNAVGANELVMAAAAEVEQFTGAPVG
                     FAGPIGLKDVPIYADPLVTQMNNFIVGANLFEKHTVNVNLGRDFKAAKIAD"
BASE COUNT          605 a          893 c          807 g          524 t
ORIGIN      
        1 ataccgcggc gaaacggtag tacagccgca cgaccggtcc atgccgccgg tgcccgatca
       61 ggctggccga gacgaaggtg agggagagga cgaagaggat cgccgccgcg ggtcccggcg
      121 cgatcgagaa gaatgcgacg gcagtcgccc cgagcagcgc gtgcccgatc cagagaatac
      181 cctgcacgat ggcgacgaat atgaggaagc gcttgggcat aaacgtgagg tatataatgg
      241 aaagccgggg gaaaggtttt ggcgggacag cttgattact cgtcgaatcc taatcgcctc
      301 cgtaatcgct cagtagaatt tgtcaacgaa ctcccgaatc gcatcgaatt gctcccgcgg
      361 catcagcatc atcgccgcgg gcagttccgg cgctatgaag gcgtaccaga tgaagtgctc
      421 tacctggccc gagtcgtctg cctcttgcgc caattcctca acctgaggcc accaagccgg
      481 ggccagtgtg tcatccaatc ccgccatgaa atcaagccag gtgacagcag cttctgcaat
      541 ctcctgcatg agactaccgc gccagtcttc tccctgacgt gattccgcgt acccgggttc
      601 atatttccaa accgcccgga ccaaggcata ggcgacggat tccgcttcaa aacgcacccg
      661 tacccctgag gtgtctcgtg tgagcgcata gtgaagggaa atatccggcc cacgccaggt
      721 tcccccggca tcttcccggt atgatttcag caccttcttg atctccatgt gattcacgtt
      781 cagcaagtgg gcgatggtga gttcttcgat ggcgcgggcg ctgtcaccct ggagccacat
      841 catatgcccc aggaaccagt gcgcctggta attgatgaaa ttcgcatcga ctgcccgctg
      901 gtaccacgcc cgtgcgctgt caagttcgtt catgccgaaa taggcatccc ccgcgtagac
      961 catcgccgga tcgaaactcg gccgcaggag aagcgcttcg gagtaagccg ccagagcggc
     1021 ctcgaagtcg ccggcctcgt acgccccttc agctatggcg aagatgacac cgagcgaatc
     1081 gtcgatttca tgtctgacca agctccggct ggagctgtca acgtacaggt ataacatgtt
     1141 tgagttaagg cgtcgcgcct tgtcgattga atccacctga acggagtcga ggtgctcaag
     1201 tacgtagacc aggtcagact gctcaatgat tttatatagg tcggcaacgc tggggatcat
     1261 gggagtctca tcagggcggt acggctgtga taacgcgggg ggtacgactg tcagtgagac
     1321 ggctgtcgca aagggcagcc aggcgaatgt gccgggggaa agtttcatga cgcccgtact
     1381 gctttcggtt ctccacacag cagaaatacc cccgccagga attgaacctg gatttccggc
     1441 tccggaggcc gacgcgttat ccattacacc acgggggcat gcacggccca ttaataccca
     1501 attcccgtcc tgtgcgcaac cagattttgt ccccattttc gcgcccccgg cccgcacggc
     1561 tcggcgtccg gcgccggccg aggccggagt ttttcagttg ctttgaaccc ctgattgcct
     1621 aatataccaa ttcgttaacg tgacccggac caaaccgaac atgaggaccc gaccatgcgt
     1681 tggacgcact cctacatccc gaccctccgc gagaaccagg ccgaggccga actgatctcc
     1741 caccagctcc tgctgcgcgg cggctacatc cgcaaactcg cctcgggcat ctacctctac
     1801 ctgcccctca tgcagcgcgt catccacaag ttctcaaaca tcgtccgcga agaaatgaac
     1861 cgcgccggcg gcctcgaaat cgccatgtcg gtcctgtgcc cggccgaggt ctggcaggaa
     1921 tcgggccgct acgggacgat cggcaaagag cagatgcgcc tccgcgaccg gcacgaccac
     1981 gagcaggttc tctgcggcac ccatgaggaa acggtcacat tcctcatccg cggcgaggtc
     2041 aaatcctacc gccagcttcc cctgaacctc taccagatcc aggtgaagtt ccgcgatgag
     2101 atccgccccc gtttcggcct catgcgcggc cgcgagttca tcatgaaaga cgcctactcg
     2161 ttcgatgccg acgaggaatc gttcgcacgc tcgtaccagg cgatggtcga cgcctatttt
     2221 cgtattttcc accgggccgg cctcgacgtc gtcaaggttg agtccgacac cggcgcgatg
     2281 ggggggaagg ccgcccacga gttcatgttg ctcgtcgaca ccgaggccgg cgaagagatc
     2341 atcatgtcgt gcgactcctg cgactacacg gccaacctcg agaaagccgc gttctgcgat
     2401 ccgctccaac cggcgcccga gcccgagacc aaacccaggg aaacggtgga cacgcctggg
     2461 gcggccacga tcgaggaagt caccgctttc ctgaaagtcc ccgcgcaccg actggtcaag
     2521 actttgctgt atatggccga cggcaagccg gtggccgcgc tcgtgcgggg ggaccgggaa
     2581 ctgaacgaaa ccaagctcaa gaacgccgtt ggggccaatg agctggtcat ggccgcggcg
     2641 gccgaggtgg agcaattcac gggcgccccg gtcgggttcg ccggcccgat cggcctgaaa
     2701 gacgttccga tctacgccga ccccctcgtc acccaaatga acaacttcat cgttggcgcg
     2761 aatctgtttg aaaaacatac agttaacgtg aacctcggac gggatttcaa ggccgctaaa
     2821 atcgccgac
//
LOCUS       DAOOKS010000214         2822 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_43566_length_2822_cov_4.142754, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000214 DAOOKS010000000
VERSION     DAOOKS010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43566_length_2822_cov_4.142754"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2459
                     /locus_tag="PK112_04385"
     CDS             <1..2459
                     /locus_tag="PK112_04385"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HPC11319.1"
                     /translation="FACDCAERACQRATADNRAYAAILAGRGWADGRMSVADARAAAF
                     AAHEAARGAVTPVSQFACRSAGHAAATAHVITHAVAAADYAAKAAPDPLEFFYGQRAP
                     SLRPYDIYSFLYPNIQAAESHLAPGGDRFFEAARRRHLNPFAAGRVQPVALWSGLVTT
                     DASGRASIDFRLPAFNGRLVLMAVAAQGNRFGSAVREVTVRENLIVQESFPRFVSPND
                     VVTGLVTVFNHTGDSGEVTVELELDGPAEAVGDKARTIALADGAEGSVAFTFKAGVVP
                     GPIAVRIRATAGADQTEAAFQLPNRPARPLSTRYGAGVVTDTAAARIVPPADVIPRTA
                     RFTLQTSSLNALQFRRQIEYLLSYPYGCVEQTTSRVFPLLYFESLARFVSPELLGGNA
                     PDYFVQEGIRRLGAMMRADGTFSFWPGGGPPSPWTSVYAGHFLLEARQAGFVVEDDLY
                     EAVRKRLLAWARGRDLTDADAEIRVYAAYALASTDQLDRKILNELRQIPIAPLSLQAK
                     FHLAGALALAGDRTAAFAIIDPIEIQPAVFPPETGGTLASGTRTSAVMLDVLARLDPD
                     NPSAAVLARDLTESARVGRWYTTQESAFALMALGRYLGSRPAADFTGRVALAGDSAYT
                     FDETGLSLTRENFLGREVTISIDGAGPCYYYWQLSGVSTSPAVEEFARGIRVAREYLD
                     ADGNPLELDSVRLGDQVICRIEAIAERDNLSHVAINDLLPAGFAVENPRLKTTPRLAW
                     IPRESAAIGNSDIRDDRVLLFVDLNPKTAVVYHYSLRAVAAGEFQVPPIAAECMYNPV
                     IAGASGSGVLTVRRPGGDGR"
     gene            2471..>2822
                     /locus_tag="PK112_04390"
     CDS             2471..>2822
                     /locus_tag="PK112_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HPC11320.1"
                     /translation="MPFRAPRRKVKLAAASLALALALLAALDRFVFPLPVERLSRPHA
                     RFVYSRDRVLLASFTSRDSFWRAPVPYNGLSPRLIESVLAVEDRWFRRHPGVNPVALL
                     GAAWDNLRAGRVVRG"
BASE COUNT          388 a         1091 c          885 g          458 t
ORIGIN      
        1 gctttgcctg cgactgcgcc gaacgcgcct gtcagcgcgc cacggcggac aaccgcgcat
       61 acgccgccat cctggccggg cggggctggg cggacggaag gatgagcgtt gcggacgcac
      121 gcgccgccgc gtttgccgcg cacgaagccg cccgcggagc cgtaacgccc gtatcgcagt
      181 ttgcctgccg cagcgcagga cacgccgcag ccacggcgca cgttattacg catgcggtgg
      241 ccgccgccga ctatgcagca aaagccgccc ccgacccgct ggaattcttc tacggccagc
      301 gcgccccgtc gctgcggcct tacgacatct actccttcct ctaccccaac atacaggccg
      361 cggagagcca cctggcgccc gggggcgacc gtttcttcga ggccgcccgc cgccgccacc
      421 tcaacccgtt cgccgccggc cgcgtccaac cggtcgcact ctggtcgggg ctggtgacga
      481 ccgacgcttc cggccgcgcc tcgatcgatt tccgcctccc cgccttcaac ggccggctcg
      541 tgctcatggc cgtggccgca cagggcaacc gtttcggctc cgccgtgcgc gaagtcactg
      601 tccgcgaaaa cctcatcgtg caggagagct tcccgcgctt cgtcagcccg aacgacgtgg
      661 tcaccggtct cgtcaccgtg ttcaatcaca ccggcgactc gggcgaggtg accgtggagc
      721 ttgaactgga cggtccggcc gaagcggtcg gcgacaaagc ccggacgatc gcgctggccg
      781 acggcgccga gggttcggtg gccttcacgt tcaaggccgg cgtcgtgcct ggtcccatcg
      841 ctgtccgcat ccgcgcaacc gccggcgccg accagacgga ggcagccttc cagttgccca
      901 accgtcccgc ccgcccgctc tccacccgct atggcgcggg cgtcgtcacc gacaccgccg
      961 ccgcccgcat cgtaccgccg gccgatgtga tcccgcgcac cgcccggttc accctccaga
     1021 cgtcgtcgct gaacgccctt cagttccgcc gccagatcga gtacctcctc tcctacccgt
     1081 atgggtgcgt tgaacagact acctcgcggg tgttcccgct cctgtatttc gagtctctgg
     1141 cgcgtttcgt cagtcccgaa ctgctcggcg ggaacgcccc cgactacttc gtgcaggagg
     1201 ggattcggcg gctgggggcg atgatgcggg cggacggcac gttcagcttc tggcccggcg
     1261 gcggcccgcc ttcgccctgg acgagcgtgt acgccggaca cttcctgctg gaggcccgac
     1321 aggcggggtt cgtggtcgag gacgatctct acgaggcggt gcgcaagcgc ctcctggcgt
     1381 gggcgcgcgg ccgcgacctg accgacgccg atgccgagat ccgcgtctac gcggcctacg
     1441 cgctcgcctc caccgaccag ctcgaccgca agattctcaa cgaactgcgc cagattccga
     1501 tcgcgccgct gtcgctgcag gcgaagttcc acctggccgg cgcgctcgcc ctcgccggcg
     1561 accgcaccgc cgccttcgcc atcatcgacc cgatcgagat ccagccggcc gtttttcccc
     1621 ccgaaaccgg gggcaccctt gcctccggca cgcggacgag cgcggtcatg ctcgatgtcc
     1681 tcgcgcgcct cgacccggac aacccctcgg cggctgtgct cgcccgcgat ctgaccgaga
     1741 gcgcccgagt cggacggtgg tacacgaccc aagagagcgc ctttgcgctc atggcgctcg
     1801 gccgctacct cgggtcgcgc ccggccgctg atttcaccgg ccgcgtcgcc ctggccggcg
     1861 actccgccta caccttcgat gaaaccggcc tgagcctcac gcgcgagaat ttcctcggcc
     1921 gcgaggtcac gatcagcatc gacggcgccg gtccgtgcta ctactactgg cagctttccg
     1981 gcgtgtcgac ttctcccgcg gtcgaagaat tcgcccgcgg gatccgggtg gcccgcgagt
     2041 atctcgatgc cgacggcaac ccgctcgaac tcgacagcgt ccgcctcggc gaccaggtca
     2101 tctgccgcat cgaagcgatc gccgagcggg acaatctctc ccacgtcgcg atcaacgatc
     2161 tcctccccgc cggattcgcc gtcgagaacc cgcgtctgaa gacgaccccg cgcctcgcct
     2221 ggatcccccg cgagagcgcc gccatcggca actccgacat tcgcgatgac cgcgtcctgc
     2281 tgtttgtcga cctcaacccg aagaccgcgg ttgtctatca ttactcgctc cgcgctgtcg
     2341 ccgccggcga attccaggtt ccccccatcg ccgccgagtg catgtacaac ccggtgatcg
     2401 caggcgcctc cggcagcggc gtgctcaccg tgcgccgccc gggcggagac gggaggtagc
     2461 ggaggacccg atgcccttcc gtgcgccgcg ccgaaaggtg aagctggcgg cggcgagtct
     2521 cgcgctcgcc ctcgccctgc tggccgcgct cgaccggttc gtctttcccc tcccggtcga
     2581 gcgcctgtcc cggccgcacg cccggttcgt ttacagccgc gaccgtgttc tcctggcgag
     2641 cttcacctcg cgcgattcgt tctggcgcgc gccggtcccg tacaacggcc tctcgccgcg
     2701 cctgatcgag agcgttttgg cggtcgagga ccgctggttt cgccggcacc cgggggtcaa
     2761 cccggtcgcc ctgctggggg cggcgtggga caacctgcgc gccgggcggg ttgtccgcgg
     2821 cg
//
LOCUS       DAOOKS010000215         2817 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_43674_length_2817_cov_3.095221, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000215 DAOOKS010000000
VERSION     DAOOKS010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43674_length_2817_cov_3.095221"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1010
                     /locus_tag="PK112_04395"
     CDS             <1..1010
                     /locus_tag="PK112_04395"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPC11321.1"
                     /translation="EVIQDLLTLSRRGRYDMTPVDLNEVVQACIDSAPFRNLTSRKPQ
                     VAVTLRLCDSVTAMNGSFPHLQKLVGNLLANAFEAIDTAGEVTVTTARRCLETLPGGY
                     TRIDPGEYVTLSVRDTGRGIPAADLDKIFEPYYIKKMVGAGGTGLGLSVVYGVVKDHG
                     GYYDVFSEPQRGSEFVVYFRTIQSPVAARPPVGDFSGRGENILVVDDAAAQRDLASEL
                     LTGLGYRVAAAENGRQALRYLDTHVADLIVLDMIMEDDFDGLDTYREILLRRPDQRTV
                     IVSGYSSTERVQEMQRLGAGAYIRKPYSLESLGRAVREELDRAVGDGAPSVLAAADPL
                     R"
     gene            complement(1215..2564)
                     /locus_tag="PK112_04400"
     CDS             complement(1215..2564)
                     /locus_tag="PK112_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11322.1"
                     /translation="MPFVDTDTTTYPKKFWMGHKQQLSHPVRSIYPRITAQDSILCVT
                     YSSREAVTKAGTWWVYRVLSFDYGLTWSEPRAIENTGDFNAGDLFPLIEGDTILLSFS
                     RQVEGIETTYQQVVAKESLKDAALTTPVVLQQSTANLHHSQLVRHQDTTWLFYVFSDW
                     DYNHLASCRSTDNGQTWDTLNFSGPSIGWPMSFQRANDHISWVGEEGVESYCRLLNPD
                     GVTWTDRVLISETPANTASQWPNACSNGLSDLHAGWFDFDGAEGGWYGYIFYTHSYDG
                     GESWGPIWSLSPTDDCEHSAFWADSQRVYAVWNDCRENGSPDFALYLRYSHDRGMSWS
                     PEIEVVDKIDPAREPDLYGQGDLVYLVWNEQHPPDWIWGIYFQVGGWYIPGDVNRSED
                     LTVSDLTFLVSYLFRGRAAPVLLGAAEMNGSAPINIADLTYFVDYLFRGGAEPVGAQ"
BASE COUNT          529 a          920 c          838 g          530 t
ORIGIN      
        1 ccgaggtcat ccaggacctg ctcacgctct ctcggcgcgg ccggtacgac atgacgcccg
       61 tcgatctcaa cgaggtcgtc caggcctgca tcgactccgc gcctttccgc aacctcacct
      121 cccgcaagcc gcaggtggcg gtcaccctcc gcctctgcga ctcggtcacc gccatgaacg
      181 gatcatttcc gcacctgcag aagctggtgg gcaatttgct ggccaacgcg ttcgaggcga
      241 tcgacacggc gggcgaggtg acggtgacaa ccgcgcggcg ctgcctggaa accctgccgg
      301 gcggttacac ccggatcgat cccggcgagt acgtcaccct ctccgtccgc gacaccgggc
      361 gcggtattcc ggccgccgac ctggacaaga tcttcgaacc gtattacatc aagaagatgg
      421 tgggggcggg gggaaccggg ctgggcctct cggtcgtgta cggcgtggtg aaggaccacg
      481 ggggatacta cgacgtgttc tccgagccgc agcggggttc cgaattcgtc gtctacttcc
      541 gcaccatcca atcgccggtg gcggcccgcc cgccggttgg cgacttctcc gggcgcggcg
      601 agaacatcct ggtggtcgac gacgctgccg cccagcgcga tctggcctcc gaactcctca
      661 ccggcctggg ctaccgtgtg gcggcggccg aaaacggccg ccaggcgctc cgctacctcg
      721 acacccacgt cgccgacctg atcgtcctcg acatgatcat ggaggacgac tttgacggcc
      781 tcgacaccta ccgggaaatc ctcctgcgcc gtcccgacca gagaacggtc atcgtgagcg
      841 gctactcctc caccgagcgc gtgcaggaga tgcagcggtt gggggcgggg gcgtacatac
      901 gcaaaccgta ctcgctcgag tcgctcggcc gggccgttcg cgaggagctc gaccgggcgg
      961 tcggtgacgg tgcgccctcc gtgctcgcgg cggccgatcc gctgcggtag cgcccgcaat
     1021 tccatttgcc tgcgtttcct cgccggtcct gccttgccgc cgtaaccggt attctacctc
     1081 aggatgacgc tatggattat tccgcccgcg agctcgtggc ctgccgcttg tcttccctcc
     1141 tcgttcctcc cgccgcgtaa tcgcgtgtat gttcaagccg aaagctattc ctctacagca
     1201 acaggcgaac caagtcactg agcgccgacc ggttcagcgc cccctcgaaa cagataatct
     1261 acgaaatagg tcagatcagc aatgttaatg ggcgcactgc cattcatctc ggccgccccc
     1321 aacagcaccg gggcagccct gccccggaag aggtagctca ccaaaaaggt caggtcgctc
     1381 accgtgagat cttccgagcg gttcacatcc cccgggatat accacccgcc cacctggaag
     1441 tagatccccc agatccagtc cggcggatgc tgctcgttcc agacgaggta caccagatca
     1501 ccctgcccgt agagatccgg ttctctcgcc ggatcaatct tgtccactac ctcgatctcc
     1561 ggcgaccagc tcatcccgcg atcgtgcgag tagcgcaggt agagggcgaa gtccggcgag
     1621 ccgttttccc ggcagtcgtt ccacaccgcg tacacccgct gggagtcggc ccagaaggcc
     1681 gagtgctcgc agtcgtcagt cggcgagaga ctccagatcg gcccccagct ctccccgccg
     1741 tcgtagctgt gggtgtagaa gatgtagccg taccagcccc cctcggcgcc gtcgaagtca
     1801 aaccagccgg cgtggaggtc ggagaggccg ttgctgcacg cgttcggcca ctggctggcc
     1861 gtgttggccg gggtctccga gatcaggacc cgatcagtcc aggtcactcc gtccgggtta
     1921 agcaggcggc agtagctctc caccccctct tctcctaccc aggagatatg gtcgttggcc
     1981 cgttggaaag acatcggcca gccgattgag gggccggaga aattcagggt atcccacgtc
     2041 tgcccattgt cagttgagcg acaggaggcg agatgattgt agtcccagtc cgagaataca
     2101 tagaatagcc aagttgtgtc ctggtgtcta accaactggg agtggtggag atttgcagtg
     2161 gactgctgca gaacaaccgg ggtcgtcaga gcggcatcct taagagattc tttggccacc
     2221 acctgctggt acgtcgtctc gatgccctcg acctgtcggg aaaaagaaag cagaatggtg
     2281 tcgccctcaa tcaatggaaa taggtcgccc gcgttaaagt ccccggtatt ctcgattgcc
     2341 cgcggctcac tccaggtcaa gccatagtca aaggagagca cgcggtagac ccaccacgtc
     2401 ccggccttcg tgaccgcctc ccggcttgag taggtgacac agaggatgga atcctgagcc
     2461 gttatgcgcg ggtagatgct gcggacggga tgagacagct gctgcttatg ccccatccag
     2521 aatttcttgg ggtacgtggt ggtatcggta tcgacgaacg gcaccgaggg cgtacgcgac
     2581 ggcgtcggat tgctattgta gacacgaccg ctaaggctgt ccggcgacac aggctccgcg
     2641 aggtcattgg ccgatccgat tgacaccaac agcaacagcc ccaccacgac ccgcgagccg
     2701 gttattggaa ataccttcac cgtccctctt ccattttcgg attacaactc attatagagc
     2761 gggagacacg ttttgtcaag gggtacccac tggttcatgc ctctggggta atatcgt
//
LOCUS       DAOOKS010000216         2810 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_43842_length_2810_cov_3.727405, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000216 DAOOKS010000000
VERSION     DAOOKS010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43842_length_2810_cov_3.727405"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="PK112_04405"
     CDS             complement(<1..634)
                     /locus_tag="PK112_04405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPC11323.1"
                     /translation="MAADETFPIASYCSSQAWGGLEMNVLRFLHWMRGRGWPAVLFAR
                     PGSPLLTWAAAWGLPARALQSRSKASDLLGARTLARMAAADSARVLILHQSRDLLMAV
                     LTKIHTGNRLKVVYQQHMHIAGDKRDLLHAWQYRRLDAFVAPAPSLARQVAERTPIRP
                     ERLHVIPLGLEADRFLRKPPRSQARRQLGLPLDVPLAGIIGRLDPKKGQHV"
     gene            complement(691..1716)
                     /locus_tag="PK112_04410"
     CDS             complement(691..1716)
                     /locus_tag="PK112_04410"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPC11324.1"
                     /translation="MNILIIGGTRFMGPVVAAQLLADGHRVTVFHRGETEQDVPAGVH
                     HIHGDRGRLADYRRQWERLRPDVVLDMMALTGPQAGELVAAMSGLAGRLVAASSCDVY
                     RNFGLILRRETGRATSGRLTEDSPLRDHEYPYRQDAQGPADPFYDYDKIHVEREIQRG
                     PLPACAIRLPMVYGPNDPKHRLYSYARRMSDRRPALLLDTVRADWRGIRGYRDNCAHA
                     LCLAVARGAGRHRVYNVGEAEALTEAAWIRAIGAALGWEGDIVTVADRDLPDYLRTPL
                     DWRPQLDVDSSRIRNELGYGEIVDLQPGLARTLEWELAHPPDHPADRFDYAQEDAILR
                     RSRPSPS"
     gene            complement(1850..2041)
                     /locus_tag="PK112_04415"
     CDS             complement(1850..2041)
                     /locus_tag="PK112_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HPC11325.1"
                     /translation="MKVRVDHELCSGDGICEELCPAVFKMNENDQADVIVDEVPPEEA
                     DAVREAAESCPEECIHIEE"
     gene            2456..>2810
                     /locus_tag="PK112_04420"
     CDS             2456..>2810
                     /locus_tag="PK112_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019735904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HPC11326.1"
                     /translation="MRFNDIVEAIGYTPLVRVNRLTDARSATVFLKMENFNPAHSVKD
                     RIGRSMIEAAERAGVLKPGMTIVEPTSGNTGIALAMVAAAKGYRCVLTMPDTMSIERR
                     ALCRQFGAEIVLTPGA"
BASE COUNT          460 a          890 c          976 g          484 t
ORIGIN      
        1 caacgtgctg ccccttcttc ggatcgagcc gcccgatgat ccccgccagc ggaacgtcga
       61 gcggcagccc gagctgccgg cgggcctgcg agcggggggg cttccggaga aaacggtcgg
      121 cctcgaggcc cagggggatg acatggaggc gttcggggcg gatgggggtg cgctccgcca
      181 cttgccgggc cagcgacgga gcgggcgcga cgaaagcatc gagccggcga tactgccagg
      241 cgtggagcag gtcgcgcttg tctcccgcga tgtgcatgtg ctgttggtag acgactttca
      301 gccggttgcc ggtgtggatc ttggtgagga ccgccatcag cagatcgcgc gactggtgca
      361 ggatgagcac gcgcgcgctg tcggcggcgg ccatgcgggc cagggtccgg gcccccagca
      421 ggtcggaggc tttcgagcgc gattggagcg cccgggcggg caggccccat gccgcggccc
      481 aggttagcag cggactgccc ggccgggcga agaggaccgc cggccagccc cgccctcgca
      541 tccagtggag aaagcgcagg acgttcatct cgagcccgcc ccaggcctgc gaggagcagt
      601 acgaagcgat cgggaaggtc tcgtcggccg ccatgcgcca atatatcggg gggccgccgc
      661 cgcggccaag ctctttgtcg cccgacccgc tcaggagggc gaggggcggc tccgccgaag
      721 gatggcgtcc tcctgcgcgt agtcgaagcg gtcggccggg tggtccggcg gatgggccag
      781 ctcccattcg agcgtgcggg ccagccccgg ttggagatcg acgatttctc cgtaccccag
      841 ttcgttgcga atgcgcgagg aatcgacgtc gagctgtggc ctccagtcga ggggggtccg
      901 gagatagtcc ggcagatcgc ggtcggccac ggtgacaata tccccctccc acccgagggc
      961 cgcgccgatc gccctgatcc acgccgcctc ggtcagcgcc tccgcctccc caacattgta
     1021 gacccggtgg cgacccgctc cccgcgcgac ggcgagacag agggcgtggg cgcaattgtc
     1081 gcggtagccg cggattccgc gccagtcggc gcgcaccgta tccagcagga gggcggggcg
     1141 ccggtcgctc atgcgcctgg cgtagctgta gagccggtgt ttgggatcgt tcggcccgta
     1201 gaccatgggc aggcggatcg cgcatgcggg cagcggcccg cgctgaatct ccctctccac
     1261 atggatcttg tcgtagtcgt agaagggatc cgccggtccc tgggcgtcct gccggtaggg
     1321 atactcatgg tcccgcagcg gggagtcctc ggtgaggcgg ccggaagtcg cgcggccggt
     1381 ttcccggcgc aggatcaggc cgaaattcct gtacacatcg caactgctcg ccgccaccag
     1441 ccgccccgcg aggccggaca tcgcggccac gagttccccg gcctgcggtc cggtcagggc
     1501 catcatgtcg agcacgacat ccggccgcag ccgctcccac tggcggcggt agtcggcgag
     1561 acggccgcgg tcgccgtgaa tatggtgaac gcccgcgggc acatcctgtt cggtctcgcc
     1621 gcgatgaaac acggtcacac ggtgcccgtc cgccaggagt tgggcggcga cgacgggtcc
     1681 catgaagcgg gtgccgccga tgatgaggat gttcatacgg gcagtgcgct gcgctgcggc
     1741 cgggtcggag ctccttgatc ccggtcgaag gggaagaaga aaagagcgcg gtcgcacgcg
     1801 gccgcgctcc aggcatatct gtcgttcccg ccgggcggga agtcagcgct cactcttcaa
     1861 tgtggatgca ttcctcaggg cagctctcgg cggcttcccg aacggcatcc gcttcttccg
     1921 gaggaacttc gtcaacgatc acatcggcct gatcgttctc attcatcttg aacacggccg
     1981 ggcagagctc ctcgcagatg ccgtcgccgc tgcacagttc gtggtccacg cgtaccttca
     2041 tcgtaacctc cttagggtca tctcggatgt atgtcgttca catgctttcg cttcacaggt
     2101 catggcgtcc cacgctcccg gctccggcgt gttgggcgcc ttcggcctga tttcgaagcg
     2161 aagcatacac aaaaaccggg tgatgtcaag ggcggctttt gacttccgtc aagccctcag
     2221 ccgatttttt ttgggcgtga ccggcggaaa tttccgggtg cgttgggcgg cgtaatttgt
     2281 tatagtatct gagggtcttc atgaagcaca tgacatttct ggggatgccc tgatgtgcgc
     2341 cgcggcggcg gccgggcact cgcgtgtccc gcgccgggcc gaacggtccg tccggggcaa
     2401 ggcccctcaa tgaactcagg tagatggacg ataacaactt ataggagtca gtcgaatgag
     2461 atttaacgac attgttgagg ctatcgggta caccccgctg gtgcgcgtca accgcctgac
     2521 cgatgcccgt tccgccaccg tgtttctcaa aatggagaac ttcaatcccg cgcactcggt
     2581 caaagaccgc atcggacggt cgatgatcga agcggccgag cgagcgggag tgctgaaacc
     2641 cgggatgacg attgtcgaac cgacctcggg gaataccgga atcgcgctgg ctatggtggc
     2701 ggcggcgaaa gggtaccggt gcgtgttgac catgcccgac accatgagta tcgaacggcg
     2761 ggcgttgtgc cggcagttcg gggccgagat tgtcctgacg ccgggagcgg
//
LOCUS       DAOOKS010000217         2809 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_43868_length_2809_cov_3.084241, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000217 DAOOKS010000000
VERSION     DAOOKS010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43868_length_2809_cov_3.084241"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1638)
                     /locus_tag="PK112_04425"
     CDS             complement(<1..1638)
                     /locus_tag="PK112_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11327.1"
                     /translation="MRHSIALVLAPVLLAGLAAAEPMSRARQEEIIDAYLYITGRAPA
                     PAQALGEGVQLTPDGAPIVKCGMAVAADLLNNRSRIDKELLQAAGIDQSLRPVLRLEE
                     TLVSPDQRFKVHYTRVGDSAVILTDNNGNSIPDYVEATAVILDSVHHNIVGVLGYPAP
                     PRDDFYAGGGDSLYDVYLLDIGAGAYGYTQPEELTGPESMWATSFMALDRNYDGLPDY
                     AGRPLDAIRVTAAHEYFHAVQFALDYFEGTVYLQPTDTVVARYWMEMSSVWMEEKLYD
                     DINDYYNYLPYFFADPRLSLQQFNSYYDLQPYANGICPIFLEQYYDDTLIYAIWKGCE
                     ELGPGPDFLTAANAEIDTFTAGAQQWPQAMREFRIWNWFTGTHADPVPQYVGYEERQA
                     YPELNPARLMRTATYPDTVPANDNPLNPLHNGAAYILLDSTKFVPPDTTYWNCTSVLD
                     SACTDSTEVPQTADWDFFRIGCVSRDTTYWVCNSWSDSVCGGRVRRSVSQDWDFSIVK
                     CSTIVNGIDTSYVELLPRYYKCTLWVDTRCDDSVQVDG"
     gene            complement(1635..2474)
                     /locus_tag="PK112_04430"
     CDS             complement(1635..2474)
                     /locus_tag="PK112_04430"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPC11328.1"
                     /translation="MVRRFIDLHLHTDCSDGLYAPSEVVRMVREAELAAFALTDHDTL
                     EGYREARKLLRPGDPELIPGLELSVESAGGELHLLAYLFDPEDAALGEALAAFRERRN
                     RRARQIVERLNELGISLSYEQVAATAGKAAVGRPHIAQTMHERGLVTAYQSAFDKYIG
                     GGKPAYVPKQNFTPAEAIRAVHAAGGVVVLAHPQIDETYRHLELLAGLGLDGIEMHHP
                     SHQQRHTEQFRHLAERHRLLVTGGSDFHGREGRYGAIGSQRVPAEYLDALRQRAQARK
                     DTL"
     gene            complement(2475..>2809)
                     /locus_tag="PK112_04435"
     CDS             complement(2475..>2809)
                     /locus_tag="PK112_04435"
                     /inference="COORDINATES: protein motif:HMM:NF016825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HPC11329.1"
                     /translation="EEQAQREAAVEPATKHAAEVPLTVMQKSLEVLRLAQTVAAKGNQ
                     NSITDAGVAGLMGMSAIEGAGYNVRINLTNLRDQDFARRLREQTAAIAAEGRAIAGEI
                     RNLVEAKL"
BASE COUNT          440 a          912 c          953 g          504 t
ORIGIN      
        1 cccgtcgacc tgaaccgaat cgtcgcaccg cgtgtcgacc cagagcgtgc acttgtagta
       61 gcgcggcagc agctcgacgt aactggtgtc gattccgttc acaatcgtcg aacactttac
      121 gatcgagaag tcccaatcct gggaaacgct ccggcgcacg cgcccgccgc acaccgaatc
      181 ggaccaggag ttgcagaccc agtaggtcgt gtcgcgggag acgcagccga tgcggaagaa
      241 atcccagtcg gccgtctgcg gcacttcggt ggaatcggtg caggcagaat cgagcacgct
      301 ggtgcagttc cagtaggtcg tatcgggcgg cacgaacttg gtgctgtcaa ggagaatata
      361 cgccgcgccg ttgtgcagcg ggttgagcgg gttgtcattc gcgggcaccg tgtccggata
      421 ggtggcggtg cgcatgagcc tggccggatt cagctcgggg taggcctgcc gttcctcgta
      481 gccgacatac tggggaaccg ggtccgcgtg cgttccggtg aaccagttcc agatgcggaa
      541 ctcgcgcatc gcctgcggcc actgctgggc gccggccgtg aacgtgtcga tctcggcgtt
      601 ggccgccgtc agaaaatctg ggcccggccc cagctcctcg caccctttcc agatcgcgta
      661 gatcaacgtg tcgtcgtaat actgctcgag gaaaatcggg cagatgccgt tcgcatacgg
      721 ctgcagatcg tagtacgagt tgaactgctg cagcgacagc cgggggtcgg cgaagaagta
      781 gggcaggtag ttgtagtagt cgttgatgtc gtcgtagagc ttctcctcca tccacaccga
      841 cgacatctcc atccagtacc gcgccacgac cgtgtcggtc ggctgaaggt agacagtccc
      901 ctcaaaataa tcgagagcga actgcacggc gtggaaatac tcgtgtgcgg ccgtgacccg
      961 gatcgcatcg aggggccgcc cggcgtagtc ggggaggccg tcatagttgc gatccagcgc
     1021 catgaacgag gtcgcccaca tggactcggg accggtcaac tcctcgggct gcgtgtatcc
     1081 gtaggcgccc gccccgatgt cgagcaggta gacgtcgtag agcgagtccc cgccgccggc
     1141 gtagaagtcg tcccgcggcg gcgccgggta gcccaggacg ccgacgatgt tgtggtggac
     1201 gctgtcgaga atgacggccg tggcctcgac atagtccgga atggagttgc cgttgttgtc
     1261 ggtcaggatg accgccgagt ccccgacccg ggtgtagtgg actttgaacc gctgatcggg
     1321 gctgacgagc gtctcctcga gccggaggac ggggcgcagc gactggtcga tgccggccgc
     1381 ctgcaggagt tccttgtcga tccggctgcg gttgttgagc aaatcggcgg cgaccgccat
     1441 cccgcacttc acgatcggcg cgccgtccgg ggtcaactgc accccctcgc cgagcgcctg
     1501 ggccggagcg ggcgcccgcc cggtgatata gaggtaggcg tcgatgattt cctcctggcg
     1561 cgcgcgcgac atcggctcgg cggccgccag gccggccagg aggaccggcg ccaggaccag
     1621 ggctatgctg tgtctcacag ggtatccttt cgtgcctgcg cccgctgccg gagcgcatcg
     1681 agatactcgg ccggcacgcg ctgcgatccg atcgccccgt agcgcccttc gcgcccgtgg
     1741 aaatcggagc cgccggtgac gagcagacgg tggcgttcgg ccaggtggcg gaactgctcg
     1801 gtgtgccgct gctggtgcga cgggtggtgc atttcgatgc cgtccaggcc gagcccggcc
     1861 agcagctcga ggtgccggta ggtttcatca atttgcgggt gggccagaac gacgacgccg
     1921 ccggccgcgt gaacggcgcg gatcgcctcc gccggcgtga agttctgctt cgggacataa
     1981 gcgggtttgc cgccgccgat gtacttgtcg aaagcgctct ggtaggccgt caccagcccc
     2041 cgctcgtgca tcgtctgggc gatatgcgga cggcccactg ccgcctttcc ggccgttgcg
     2101 gccacttgct cgtagctcag ggagatcccc agttcgttca gcctttctac gatctgcctc
     2161 gcccgccggt tccggcgctc gcggaacgcg gcgagcgcct cgccgagcgc ggcgtcctcc
     2221 gggtcgaaca ggtacgccag aaggtgcaac tccccccccg cgctctcgac tgacagctcc
     2281 agccccggga tcagttccgg gtcgccgggc cggaggagtt tgcgcgcctc gcggtacccc
     2341 tccagggtgt cgtggtcggt gagggcgaag gccgcgagct cggcctctcg caccatgcgc
     2401 accacctcgg agggcgcgta gaggccgtcc gagcagtcgg tgtgcaggtg gaggtcgatg
     2461 aaccggcgca ccatctacag cttcgcctcg accaggttcc ggatctcccc ggcgatcgct
     2521 ctgccctcgg cggcgatggc ggccgtttgt tcgcgcaggc ggcgggcaaa atcctggtcg
     2581 cggagattgg tcaggttgat ccggacgttg tacccggccc cttcgatggc cgacatcccc
     2641 atcagcccgg ccaccccggc gtcggtgatc gaattctggt tccccttggc ggcgactgtc
     2701 tgggccagcc ggagcacctc gagtgatttc tgcatcacgg tcagcggcac ctcggcggca
     2761 tgcttggttg ccggctcgac cgccgcctcc cgctgcgcct gctcctcgt
//
LOCUS       DAOOKS010000218         2796 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_44161_length_2796_cov_4.518789, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000218 DAOOKS010000000
VERSION     DAOOKS010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44161_length_2796_cov_4.518789"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1155
                     /locus_tag="PK112_04440"
     CDS             <1..1155
                     /locus_tag="PK112_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11330.1"
                     /translation="IAVRKADGYLPDNTTDPSRPRSYLFWRDMLLNWNPDLNFNEYDY
                     IYQGRSYNNTNYTNGIPLVKLLQYKVVVLYNDHIVAPPITKEGYMKPVITGLDAGVNA
                     WSICRSVAGGDGNKSHMPPGDPQLIFNQVSNKWAMAYKRYFAVNSGLYTGWFQHANRL
                     RDPRPNAPWALPAFDVQDCIGAYSLQPAQWPDIEYDSALVHDLYEWGSLVPDTSTPDP
                     SDRIQYYYNKKLPYLPEINWCQARYGAEIMYLYKSYYRSRGVAHPLGVYEGISGSFNF
                     DFEGAPCGHRYSTGLYRTVHMNLTPFALKRGPNPTSGPVAQLFRAVMDYLYDPNIQQP
                     VAKLRYHDADPTAAADLSVERDRYWKRCDQEAYEKGLDLSAHALANLHRR"
     gene            complement(1216..>2796)
                     /locus_tag="PK112_04445"
     CDS             complement(1216..>2796)
                     /locus_tag="PK112_04445"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPC11331.1"
                     /translation="RGLAPLLRTALAVGLGLAIAIGPIFIRNLAVAGDPVLIASQGGI
                     NLYIGNNPEADGVSAVLPEPLGFNWQIRDVVHIAEQAAGRPLAPGEVSDYWAQRARTW
                     IAAHPLSFLRLYAEKLYWNISHREISNNRALGPFFALIPLLRYNPLSFGLLFALALAG
                     LAAALRTNARLRLIALILLLYVAASALFFFNSRFRLPVLPLYFILAATGVFGLAEAVR
                     RRRGLVLPAAALTVGAGLSFLPLVSLPSGVSPQAIMSAANYAYGQGDFRRALELSAEA
                     AAVDSTYPEVNLNLGNAWLRLGDAAAARAAWEREARFHPGRPKALTNLASLALLEGRF
                     ADAQAEGQRALALRPYDYDANLVWLRAVFAADTTAPEQLVAEVQAAAARTGGDVYLLN
                     EAGILLTQRGLAETARATLLAALSAPPPPIEMDDAAFAPNFRHSRERMREQHAQAAYQ
                     LGYLAALASRFGEAVEFSRRAVELDPGLAEAWVNLVGACAAAGRIDEARTTLREALAR
                     FPNRPELRELDRRLAPRQ"
BASE COUNT          514 a          902 c          952 g          428 t
ORIGIN      
        1 atcgcggtcc gcaaggccga cggataccta ccggacaaca ccaccgaccc gagccgtccg
       61 cgcagttacc tgttctggcg cgacatgctc ctgaactgga acccggattt gaatttcaac
      121 gagtacgact acatctatca gggccggtcg tacaacaaca ccaactacac caatggaatc
      181 ccgctggtga agctgctgca gtacaaggtc gtggtgttgt acaacgatca catcgtggcg
      241 ccgccgatca cgaaagaggg ctacatgaag ccggtcatca cgggtctgga tgccggcgtg
      301 aatgcctggt cgatttgccg ctcggtcgcg ggcggcgatg ggaacaagag ccacatgccc
      361 ccaggcgacc cgcagttgat cttcaaccag gtcagcaaca agtgggccat ggcatacaag
      421 cggtactttg cggtgaacag cggcttgtac accgggtggt tccagcacgc caaccggctg
      481 cgggacccgc gccccaatgc gccatgggct ctgcccgcat tcgatgttca ggattgcatc
      541 ggcgcctaca gcctgcagcc ggcgcaatgg cccgatatcg agtatgactc cgcgcttgtg
      601 cacgatctct acgagtgggg gtcgctggtc ccggatacct ccacgccgga tccctcggac
      661 cggatccagt actactacaa caagaagctt ccctatctgc cggagatcaa ctggtgccag
      721 gcgcggtacg gggcggagat catgtatctg tacaagtcgt actaccggtc gcggggcgtt
      781 gcccatccgc tcggtgtgta tgagggcatc tccggctcct tcaacttcga tttcgaaggg
      841 gcgccgtgcg gccaccgcta cagcaccggc ctttaccgga cggttcacat gaacctgacg
      901 ccgtttgcgc tcaagcgcgg gccgaacccg acttccggtc cggtcgccca gctcttcagg
      961 gctgtcatgg attacctcta cgatccgaat atccagcagc cggtcgcgaa gctgcggtat
     1021 catgatgcgg acccgacggc ggcggcggat ctctccgtcg agcgggatcg ctactggaag
     1081 cgctgcgacc aggaagcgta cgagaagggt ctggatctca gcgcccacgc gctggccaat
     1141 ctgcaccggc gctgaccggc cgccgttatt tgaccgttac cgagaccgga gccgtcggct
     1201 ccggtctctt tttgtttatt gccggggcgc caggcggcgg tcgagctccc ggagttccgg
     1261 gcggttgggg aagcgggcga gcgcctcgcg gagggtggtt cgcgcctcgt cgatgcgccc
     1321 cgctgcggcg caggcgccga ctaggttcac ccaggcctca gccaggccgg ggtcgagttc
     1381 caccgcccgg cgcgagaatt cgacagcctc cccgaagcgg ctagcgagag cggcgagata
     1441 gccgagctgg taggccgcct gggcgtgctg ctcgcgcatg cgctcgcggc tgtgacggaa
     1501 attgggagcg aaagcggcat cgtccatctc gatcggcggc ggcggcgcgg acagggccgc
     1561 caggagcgtc gcgcgcgccg tctcggccag cccgcgctgc gtgagcagaa tgccggcctc
     1621 gttgagcagg tagacgtcgc cgcccgtgcg cgccgcagcg gcctgaacct cggcgacaag
     1681 ctgctcaggc gctgtcgtat ccgccgcgaa cacggcccgc agccaaacga gattggcgtc
     1741 gtagtcgtag gggcggagcg ccagcgcccg ctgcccctcc gcttgcgcgt cggcgaaacg
     1801 cccctcgagc agggccagcg aggcgaggtt ggtcagagcc ttgggtcggc cagggtggaa
     1861 ccgggcctcc cgctcccagg cagcccgggc cgcagcggcg tcgcccaaac gcagccaggc
     1921 gttgcccaga ttcaggttca cttcggggta ggtggagtcg accgcggccg cttcggccga
     1981 gagttcgagc gcgcggcgga agtccccctg tccgtaggcg taattggcgg cgctcatgat
     2041 cgcttgggga gacacaccgg acgggagcga caccagtggc aggaacgaca gcccggcgcc
     2101 aactgtcaga gccgcggccg gcagcaccag cccccgccgc cgccgaacag cctcggccag
     2161 cccgaacaca ccggttgccg ccaggatgaa atacagcggc agcaccggca ggcggaagcg
     2221 gctgttgaag aagaacagcg ccgaggcggc gacatacagc aggaggataa gcgcgatcag
     2281 gcgaagacgg gcgttggtcc gcagcgccgc ggccagaccg gccagagcca acgcaaagag
     2341 cagaccgaaa ctcagcggat tgtagcgcag cagcgggatg agcgcgaaga acggcccgag
     2401 cgcgcggttg ttggaaatct cccggtgcga gatgttccag tagagcttct ccgcataaag
     2461 gcggagaaac gagaggggat gggcggcgat ccacgtgcgg gcgcgctgcg cccagtagtc
     2521 ggagacttcg ccgggtgcga gcggacggcc ggcggcctgc tctgcgatat gcacgacatc
     2581 ccgaatctgc cagttgaaac ccagcggttc gggcaggacc gcggagacgc cgtcggcctc
     2641 agggttgttg ccgatgtaca ggttgatgcc tccctgcgag gcgatcagca ccggatcgcc
     2701 ggcgaccgcg aggttgcgga tgaagatcgg tccgatcgca atcgccaggc cgaggccgac
     2761 cgcgagcgct gtgcgcagga gcggcgccag gccgcg
//
LOCUS       DAOOKS010000219         2786 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_44388_length_2786_cov_3.165141, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000219 DAOOKS010000000
VERSION     DAOOKS010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44388_length_2786_cov_3.165141"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="PK112_04450"
     CDS             <1..279
                     /locus_tag="PK112_04450"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPC11332.1"
                     /translation="ENLMPGRFELAQNYPNPFNPSTTIGFTLPTAQDVQLVVYNVLGQ
                     EIATLVNGRMAAGSHQVTFEASGRPSGVYFYRLTYEDGVETRKMTLVK"
     gene            361..2073
                     /locus_tag="PK112_04455"
     CDS             361..2073
                     /locus_tag="PK112_04455"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HPC11333.1"
                     /translation="MTQAVDNPAPPKAVRIGPYLLTAKIGQGGIAVIYKGRQESLDRD
                     VAIKILSPNLSTDPDIVRRFERESMVIARLNHPNIVHIIDKGEAGGRYYFVMDYIDGT
                     SLREVIDSAKIPLRTKLEMIVQVCKALDYAHKNGVIHRDIKPANILIDRQGNALVADF
                     GIAQIVGTPEAEMTASDVVMGTLSYMSPEQKVSSAGVDHTTDIYAVGVILYEILCGKK
                     PLGKFKLPSEITADLGEAYDGIISKCLAQDRGERYQAAVELKDALLEAMGGGSLRGDT
                     SDLTVSGTESFLGKCRYLDTIRETQFGSTILVENRVNKRLYVIKKHHKGEAGRKEAKL
                     LATLKHEHIVDIYGAGGDRKSTVIIAEYMQGGSLADRMVRKYDWEKALAIIRQVAAAV
                     DFAHKNNILHGNLRPSNILFDKDDVVKLSDFALPIHYDGPKQKNWYSPPERKSSRQGD
                     VYGIGVILHQMLTGRNPRYDENDNLDLDDIRMFLPEEIAAMLDRLLAIRIARRYRSCA
                     EFLLDWEDFDRRRQEERRQQESRAAAAPAAVPGKPVWLYLAIGAGIAFALFTVLYLAG
                     VFSL"
     gene            2220..>2786
                     /locus_tag="PK112_04460"
     CDS             2220..>2786
                     /locus_tag="PK112_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11334.1"
                     /translation="MRREFIGVGIIYALATGLVLLASTVRPRGLWAFSAASYFGDPWA
                     WAPAAAAVLWAAAVLLYGRRAGDGGDASDGGRRFAWWAVSHTALMAALFIGLRARTHF
                     LGDGYTLLGTYATENPLIKSRALGEALVHLWVRDLVGGPTKAAALLSFQVLAVGAGLI
                     YLGLVWWAAARLFEHRRERLLFAVGMTTP"
BASE COUNT          508 a          872 c          933 g          473 t
ORIGIN      
        1 gaaaacctga tgccggggcg gtttgagctg gcgcagaatt atccgaatcc gttcaacccg
       61 agcacgacga tcggcttcac tttgccgacc gcgcaggacg tgcagttggt tgtctacaat
      121 gtacttgggc aggagatcgc cacgctggtg aacggtcgga tggcggccgg gagccaccag
      181 gtgacatttg aggcctcggg gcggccgagc ggcgtctatt tctaccggct gacctacgag
      241 gatggggtcg agacccgaaa aatgacgctc gtcaaataag aaatcaggcc ttcgtgccga
      301 tgattacagg gatagcggga ttctccgcta tccctgtttt tctttgccga tgggcacgat
      361 atgacgcaag ctgtcgacaa cccggcgccc cccaaggcgg tccggatcgg gccgtacctg
      421 ctgacggcga aaatcggcca gggagggatc gccgtcatct acaagggccg gcaggaatcg
      481 ctcgaccggg acgtcgcgat caagattctc tcgccgaatc tttccaccga cccggacatt
      541 gtccgccgct tcgagcgcga gtcgatggtc atcgcccggc tcaaccaccc caacatcgtc
      601 cacatcatcg acaaggggga ggcgggcggc cgctactact tcgtcatgga ctacatcgac
      661 gggacctcgc tgcgggaggt gatcgactcg gccaagatcc cgctgcggac gaaactcgag
      721 atgatcgtgc aggtgtgcaa ggcgctcgac tacgcccaca agaacggcgt gatccaccgc
      781 gacatcaagc cggccaacat cctcatcgac cgccagggca atgccctggt ggcggatttc
      841 ggaatcgccc agattgtcgg gacgccggag gccgagatga cggcttctga cgttgtgatg
      901 gggacgctgt cgtacatgtc gcccgaacaa aaagtcagct cagccggggt ggaccacacc
      961 accgacatct acgccgtggg cgtgatcctg tacgagatcc tgtgcgggaa aaagccgctc
     1021 gggaagttca aactcccttc ggagatcacg gccgacctgg gagaggccta cgacgggatc
     1081 atttcgaagt gcctcgccca ggaccggggc gagcggtacc aagcggcggt cgagctcaag
     1141 gacgcgctgc ttgaggcaat ggggggcggg agcctgcgcg gcgacacctc cgacctgacc
     1201 gtctccggga ccgaatcgtt tctggggaaa tgccggtacc tcgacaccat ccgcgagacc
     1261 cagttcggct cgaccatcct ggtggaaaac cgggtcaaca agcggctcta cgtgatcaag
     1321 aaacatcaca agggagaggc ggggcgcaag gaggccaagc tcctggcgac gctcaagcac
     1381 gagcacattg tcgatatcta cggagcgggc ggggaccgga agtcgacggt gattatcgcg
     1441 gagtacatgc agggcggctc gctggccgac cggatggtgc ggaagtacga ctgggagaaa
     1501 gcgctcgcga tcatccgtca ggtcgccgcc gccgtcgact tcgcccacaa gaacaacatc
     1561 ctccacggca atctccgccc ctccaacatc ctgtttgaca aagacgacgt cgtgaagctc
     1621 tccgatttcg ccctgccgat tcactacgac ggtcccaagc agaagaactg gtacagcccg
     1681 ccggagcgga agagctcgcg ccagggggat gtgtacggga tcggcgtgat cctgcaccag
     1741 atgctcaccg ggcgcaaccc gcggtacgac gagaacgaca acctggatct cgacgacatc
     1801 cgcatgttcc tgcccgaaga gatcgcggcc atgcttgacc ggctgctggc gatccggatc
     1861 gcgcggcggt acaggtcgtg cgcagagttc ctcctggact gggaggattt cgaccgccgg
     1921 cggcaggagg agcgccggca gcaggagagc cgcgcggcgg cggcgccggc cgcggtcccc
     1981 ggaaaaccgg tctggctcta cctggcgatc ggagccggga ttgccttcgc cctgttcacc
     2041 gtgctctacc tcgccggcgt cttcagcctc tgactacccc actcaccggc cggtcctcac
     2101 ccgaggccgg cccattctcc gccgaccagg gccgccggga aagcttgtct tgcacccgcc
     2161 gccggtgtat actggcgtaa gtcttcctgc ggagggaaca cggcgccatg gcggcaacaa
     2221 tgcgaagaga gttcatcggg gtcggcatca tctacgcgct cgctaccggg ctggtgctcc
     2281 tcgcttcgac cgttcggccg agggggctct gggctttctc ggccgcgtcg tacttcgggg
     2341 atccgtgggc gtgggcgccg gcggcggcgg ccgtgctgtg ggccgccgcg gtccttctct
     2401 atgggcggcg ggccggcgac gggggggacg cgtccgacgg cggtcgccgt ttcgcctggt
     2461 gggcggtcag ccacacggcg cttatggcgg cgctgtttat cggcctgcgg gccagaaccc
     2521 attttctcgg cgacggctac acgctgctgg ggacgtacgc cacggagaat ccgctcatca
     2581 agtcgcgggc gctcggggaa gcgctcgtgc acctgtgggt gcgcgacctg gtcggcgggc
     2641 cgaccaaggc ggcggcgctg ctgagttttc aggtgctggc ggtgggggcc gggctgatct
     2701 acttagggct ggtctggtgg gcggccgcgc ggctgtttga acaccggcgc gagcgcctgc
     2761 tctttgcggt cgggatgaca accccg
//
LOCUS       DAOOKS010000220         2762 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_44912_length_2762_cov_3.364610, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000220 DAOOKS010000000
VERSION     DAOOKS010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44912_length_2762_cov_3.364610"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2762)
                     /gene="porU"
                     /locus_tag="PK112_04465"
     CDS             complement(<1..>2762)
                     /gene="porU"
                     /locus_tag="PK112_04465"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HPC11335.1"
                     /translation="PYETKNVYWLTVREGQGVRMATETVDPSAPADTTITTFTRVMHL
                     EQDNLLKEDAGGAIDDYYIWYWTTGPTVQLSAPSVGPAPGTSAQLYCRASTRDPDVSP
                     EIGYVTARVNGAAAAPNYQNQFHCSFRVDNLTDGLNTVNLTMWGADGVLPYLDYVELT
                     YQSFLAPLGGVLDVPLGGFEGTARLEAVDNFSGDLAVLRLDDPGRPVRLAGYTRAAGT
                     IAFTAALAGEGPNRFYLAETAGAFGPLGIERSTPADLYAIGEQTDMIIVTSRQLVPAL
                     AEYVERAAGRGISSRVVAVEDIMENFSFGMYDPTAIRDYLKYAYENYPSPPPHTVLLA
                     GDGSFDYTNALGTGVPNVVPPYIRPDDRSAADDNYVYFGRYGILDSDTSYILVGDRGL
                     DMMVARWPVQTAGEIATIVEKSRRYETSAALGLWRSVITVVADDEVADNQTSETFHVE
                     QAEELLAGHVPRTLHQKKIYLYDFPRVGREKPGANDAIVRQINEGAVVVDYIGHGNPD
                     VWAHEHVFWRDRDIPRLSNSDRLPLFFAASCAIGAFDDPAREAMAEELVVYPNGGGIA
                     VISAMREVWSTQNADLNQKVFDLLLTTDSLTIAEALYMGKLERQYNGGSVPYEILNDR
                     CYEYFGDPLVRLGLPRLRIAFDNPPDSLVALGRSTIAGRVLDREGRPVAADGRLVIEA
                     YDSERERQYRLASGNVVSYRLTGPAIYRGTATIRSGEFEFAFVTPLDVGYGGTGAQIM
                     TYAELAGTDGVGVIDSLSIAAAVDTTHRDETGPEIAYGFVHSVGFSDGGTVATDDVLR
                     VTLADSSGINLTGGLGHGITLVLDDRSETMADLTDRFACDQDDYTRGQLEYPLAGLSP
                     GRHTFKIKAWDNANNSATAAFTATVAAAGGPALVDLLNYPNPMRERTRFYYRLTTAVE
                     RVCLEI"
BASE COUNT          434 a         1022 c          836 g          470 t
ORIGIN      
        1 aatctccagg cagacccgct ccacggccgt cgtcaaccgg tagtaaaacc gggtccgctc
       61 ccgcatcggg ttggggtagt tgagcaggtc gaccagcgcc ggccccccgg ccgccgccac
      121 cgtcgccgtg aacgccgccg tcgccgaatt gttggcgttg tcccacgcct tgatcttgaa
      181 cgtgtgccgc cccggcgaca gccccgccag cgggtactcc aactgccccc gcgtataatc
      241 gtcctggtcg caggcaaacc ggtccgtcag gtccgccatc gtctccgacc ggtcgtccag
      301 caccagcgta atgccgtgcc ccaaaccgcc ggtgaggttg atccccgagg aatccgccag
      361 cgtcacccgg agaacgtcgt ccgtggccac cgtcccgccg tcgctgaaac caaccgagtg
      421 gacaaacccg taggcgatct ccggcccggt ctcgtcgcgg tgcgtcgtgt ccaccgccgc
      481 cgcaatcgag agcgagtcga tcaccccgac cccgtccgtc cccgccagct cggcgtaggt
      541 catgatctgc gcccccgttc cgccgtagcc gacatcgagc ggcgtgacga aggcgaattc
      601 gaattcgccg ctgcggatcg tcgccgtgcc ccggtagatc gcggggccgg tcaggcggta
      661 ggacaccacg ttcccgctcg ccagccggta ctgccgctcg cgctccgaat cgtaggcttc
      721 gatgacgagc cggccgtcgg ccgccaccgg ccgtccctcc cgatccagca cccgcccggc
      781 gatcgtcgac cgccccaggg ccacgagact gtcgggcgga ttgtcaaacg cgatccgcag
      841 cctcggcaga ccgagccgca cgaggggatc gccgaaatac tcgtaacacc ggtcgttgag
      901 aatctcgtag ggtacgctcc ccccgttgta ctgccgctcg agttttccca tgtacagcgc
      961 ctcggcgatc gtgagactgt ccgtagtcag gagaaggtcg aacactttct gattcaggtc
     1021 ggcgttctgc gtcgaccata cttcgcgcat cgccgaaatc accgcgatgc ccccgccgtt
     1081 cgggtagacg accagttcct ccgccatcgc ctcgcgcgcc ggatcatcga atgccccgat
     1141 cgcgcacgag gccgcgaaaa acagcggcag ccggtcggag ttgctcagcc gggggatgtc
     1201 gcggtcccgc cagaacacgt gttcgtgcgc ccacacgtcc gggttcccgt ggccgatgta
     1261 atccacgacc accgccccct cgttgatctg gcgcacaatc gcgtcgttgg cccccggctt
     1321 ctcccgcccg acccgcggaa aatcgtacag atagatcttc ttctggtgga gcgtccgggg
     1381 cacgtgcccc gcgagcagtt cctccgcctg ctccacgtgg aacgtctcgc tcgtctgatt
     1441 gtcggcgacc tcatcgtcgg ccaccaccgt aatcaccgac cgccacaggc cgagcgccgc
     1501 gctggtctcg tagcggcggc tcttctccac gatcgtcgcg atctcccccg ccgtctgcac
     1561 cggccagcgc gccaccatca tgtccagccc gcggtccccg accaaaatgt agctcgtatc
     1621 cgaatcgagt atgccgtacc gtccgaaata gacgtagttg tcgtccgccg ccgaccggtc
     1681 gtccggccgg atatacggcg gcaccacgtt cggcactccc gttcccaggg cattggtgta
     1741 gtcgaaactg ccgtcccccg ccagcagcac cgtgtgcggc ggcggcgacg gatagttctc
     1801 gtaggcgtac ttcaggtagt cccggatcgc ggtcggatcg tacatcccga agctgaaatt
     1861 ctccatgatg tcctcgaccg cgaccacccg gcttgagatc ccccggccgg ccgcccgctc
     1921 cacgtactcg gccagggccg ggaccagttg tctcgaggtc acgatgatca tgtcggtctg
     1981 ctcgccgatc gcgtacaggt cggccggggt gctccgctcg attcccaggg ggccgaaggc
     2041 gccggccgtt tcggccaggt agaatcggtt cggtccctcg ccggccagcg ccgcggtgaa
     2101 ggcaatcgtg cccgccgcgc gcgtgtaccc ggccagccgc accggccggc ccgggtcgtc
     2161 cagccggaga accgccaggt ccccgctgaa attatcgacc gcctccagcc gcgccgtccc
     2221 ttcgaacccg ccgagcggca cgtcgagcac cccgcccagc ggcgcgagga aactctggta
     2281 ggtcagctcc acgtagtcca ggtaggggag cacgccgtcc gccccccaca tggtgagatt
     2341 caccgtgttg agcccatcgg tgagattgtc cacccggaaa ctgcagtgga actggttctg
     2401 gtaattcggg gccgcggccg cgccgttgac ccgcgccgtc acgtagccga tctccgggga
     2461 gacgtcggga tcgcgcgtcg aggcccggca gtacagttgc gccgaggttc cgggcgccgg
     2521 cccgacgctc ggcgccgaca gttgcaccgt cggcccggtc gtccagtacc agatgtagta
     2581 atcgtcgatc gccccgccgg cgtcctcctt cagcaggttg tcctgctcca gatgcattac
     2641 ccgggtgaaa gtcgtgatcg tggtgtccgc gggcgccgag ggatcgaccg tctccgtggc
     2701 catccgcacc ccctgcccct cccggaccgt cagccagtac acattcttcg tctcgtaggg
     2761 at
//
LOCUS       DAOOKS010000221         2761 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_44937_length_2761_cov_3.330377, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000221 DAOOKS010000000
VERSION     DAOOKS010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44937_length_2761_cov_3.330377"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="PK112_04470"
     CDS             <1..274
                     /locus_tag="PK112_04470"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
                     /protein_id="HPC11336.1"
                     /translation="DANGNGVPDECEAVCTEAYCRCPVCGDVNGDGQVKVSDLTLFVS
                     YLFRGGSAPVPLAVADVNGDGQVKVSDLTLFVSYLFRGGPPPTCGF"
     gene            complement(232..1155)
                     /locus_tag="PK112_04475"
     CDS             complement(232..1155)
                     /locus_tag="PK112_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11337.1"
                     /translation="MNLTFAFTQRNALVIGLDQVLIRNRINLPWLQAQLDTTAAEHIF
                     VFGHMPAFKVDHDDCLDDYPGERDTFWNSLAAAGARVYFCGHDHFYDHARLADSDSDS
                     ADDLHQYVVGTAGAPLYAGGAYDGANGIWTPMLVHHEKEYGYVLVELDGPTAMLTWKH
                     RTAPGVYEDGGDRWPYSVPTAGCCGGDERRGDIDGRCLPECITVNDLAQFIAYMFAGG
                     RPPACAAEADIDASGGLDVADLTALLGFVYRGGAPRQPCRQARPGCRARRRPRQSRAR
                     ITGRGFLPQGVNSIPPGDQKPQVGGGPPRKR"
     gene            1520..>2761
                     /locus_tag="PK112_04480"
     CDS             1520..>2761
                     /locus_tag="PK112_04480"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPC11338.1"
                     /translation="MAEKYIFHMYGVNKFYGQKQVLRDINLSFYPGAKIGIVGENGAG
                     KTTVLNIMAGRDTEFIGHAFITPGYRAGMVEQEPQLDPQLTVRQTIESAFGETKALLD
                     EYNRLADSMAAPADDAAMQKAMDRMGVLQDKIEAVDAWNLDQRLKVAADALCLPEDDR
                     IVGTLSGGERRRVALCKVLLEKPDLLLLDEPTNHLDAETINWLEEQLREYPGTVIIVT
                     HDRYFLDNVTRWILELDGGHGIPWEGNYSSWLEQKLAALAESEKKNSPRARALERELS
                     WIRMSRGDRTELTRARMREYEQLVARETAAAKGAEAVIRIAPGPELGSQVIEFREVGM
                     GYDSRRLFENLSFAVPRSAVVGLVGPNGAGKTTLLKMVTGDVAPSEGQVVIGSTVRLA
                     YVDQEREALAGGRSLIEEIGGG"
BASE COUNT          485 a          855 c          982 g          439 t
ORIGIN      
        1 cgacgccaac ggcaacggcg ttcccgacga gtgcgaggcc gtgtgcacgg aggcgtactg
       61 ccgctgcccg gtgtgcggcg acgtgaacgg cgacgggcag gtgaaggtgt ccgacctgac
      121 cctgttcgtg tcgtatctgt tccgcggcgg atccgcgccg gttccgctcg cggtggccga
      181 cgtgaacggt gacgggcagg tgaaggtatc tgacctgacc ctgttcgtgt cctacctctt
      241 ccgcggcggt ccgccgccga cctgcggttt ctgatccccc ggtgggatag aattgacgcc
      301 ttggggcaaa aagccgcgtc cggtgatccg ggcgcggctt tgccttggtc gacgccgggc
      361 gcggcagccg gggcgcgcct ggcggcaggg ctgccgcggc gcgccgccgc ggtatacgaa
      421 accaaggagg gcggtgagat cggccacgtc gaggccgccg gaagcatcga tgtcggcctc
      481 ggccgcgcac gccggcggcc ggccgccggc gaacatgtac gcgatgaatt gggcgaggtc
      541 gttgacggtg atgcactccg gcaaacaccg accgtcgata tcgccgcgcc gctcgtcgcc
      601 cccgcagcat ccggccgtcg gcaccgagta cggccaccgg tcgccgccgt cctcgtagac
      661 gccgggggcg gtccggtgtt tccacgtcag catcgccgtc ggaccgtcga gctccaccag
      721 cacgtagcca tactccttct cgtggtgcac gagcatcgga gtccagatcc cgttggcgcc
      781 gtcgtacgcc ccgccggcgt agagcggcgc tccggcggtg ccgactacat actggtgaag
      841 gtcgtcggcg gaatcggagt cgctgtcggc cagacgggcg tgatcgtaga agtggtcgtg
      901 gccgcagaaa tagacgcgcg ccccggcggc cgccaggctg ttccagaacg tatcgcgctc
      961 gcctgggtag tcatccaggc aatcgtcgtg atcaaccttg aaggcaggca tgtgaccgaa
     1021 cacgaagatg tgctccgccg cggttgtgtc caactgcgcc tgcagccagg gcaggttgat
     1081 ccggttccga atgagcacct ggtccaggcc gatgaccagg gcattgcgtt gggtgaaggc
     1141 gaaggtgaga ttcacctcgt tctccggtcc gttggcgggc agcgcatagg gaccggggaa
     1201 cacagtgtgc gagggagcaa ggctggcccg tcgtggtttc cccggcacgg gaacaccccg
     1261 atgccggcat cgtagagagg ctgggtcaga gcgcgccaga ggagggcgaa ggctccgagc
     1321 gcaacggttc tgctgacgaa tgacatcttg ctgctcctgc cgaaagaggg ctgtctgttg
     1381 ggatctccgc cgccatgtgc cggatttgct gcggggagtc aacaaccctc tcgcccgccc
     1441 gtttaggctc ttgcgccgcc tgcaacggtg tgttaccatg gcacttgcca cactgtcgat
     1501 gggaacggag aagaatcgta tggccgagaa atacatcttc cacatgtacg gcgtcaacaa
     1561 gttctacggg cagaagcagg tgctccgcga catcaacctc agcttctacc cgggggccaa
     1621 gatcgggatc gtcggcgaga acggcgccgg aaaaaccacc gtactgaaca tcatggccgg
     1681 ccgcgacacc gagttcatcg ggcacgcttt tatcacaccc ggctaccggg ccgggatggt
     1741 cgagcaggag ccgcagctcg atccccaact gactgtccgc cagaccatcg agtcggcctt
     1801 cggggagacc aaggctctcc tggacgagta caaccggctg gccgacagca tggcggcgcc
     1861 ggccgacgac gcggcgatgc agaaggcgat ggaccggatg ggcgtgctgc aggacaaaat
     1921 cgaggccgtc gacgcctgga atctcgacca gcggctcaag gtggcggccg atgcgctgtg
     1981 tctgcccgag gacgaccgga ttgtcggcac gctgtcgggc ggcgaacggc ggcgggtggc
     2041 gctgtgcaag gtgctcctgg aaaaacccga cctgctgctg ctcgacgagc cgaccaacca
     2101 cctcgacgcc gagacgatca actggctgga ggagcaactg cgggaatacc cgggaaccgt
     2161 gatcatcgtg acgcacgacc ggtactttct cgacaacgtg acccggtgga ttctcgagtt
     2221 ggacggcggg cacgggatcc cgtgggaggg gaactacagt tcctggctgg agcagaaact
     2281 tgcggcgctg gccgaatcgg agaagaagaa ttcgccgcgg gcccgggcgc tggagcggga
     2341 gttgtcgtgg attcggatga gccgggggga ccgcaccgag ctgacccgcg cgcgcatgcg
     2401 cgagtatgag caactggtgg cgcgcgaaac ggcggccgcc aaaggggccg aggcggtcat
     2461 ccgcatcgcc cccggccccg aactgggaag ccaggtgatc gagttccgcg aggtcggcat
     2521 gggatacgac agccggcgcc tttttgagaa cctctcgttc gccgtccccc gcagcgccgt
     2581 ggtcgggctg gtcggaccga acggcgccgg caaaacgacg ctgctgaaga tggtcaccgg
     2641 tgacgtggcg ccgtcggaag gacaggtggt gatcggctcg acggtccggt tggcgtatgt
     2701 cgatcaggag cgtgaggcgc tcgcgggcgg ccggagcctg atcgaggaaa tcggcggcgg
     2761 g
//
LOCUS       DAOOKS010000222         2753 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_45111_length_2753_cov_3.215715, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000222 DAOOKS010000000
VERSION     DAOOKS010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45111_length_2753_cov_3.215715"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /locus_tag="PK112_04485"
     CDS             complement(<1..733)
                     /locus_tag="PK112_04485"
                     /inference="COORDINATES: protein motif:HMM:NF023495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rnase Y domain-containing protein"
                     /protein_id="HPC11339.1"
                     /translation="MDKIIIVAAAAVVAGVIGYFLSWRAAQRMNREKLAAATVEAERV
                     VEEAKKEAEIRRKEARLEAREEFLKLKGDFEREVAARRQELDAADKRLSELDTNLGRK
                     TEHLDAKEKDLVNREKATQAREKGIALREEELDQIIARQNERLQKIAQMTPEEAKKQL
                     MDNMINAARMEAAQYIKEIREKAEADAEKEAREIILSSIYRCAADHTVETTVSVVNLP
                     NDEMKGRIIGREGRNIRSFETATGID"
     gene            complement(834..1136)
                     /locus_tag="PK112_04490"
     CDS             complement(834..1136)
                     /locus_tag="PK112_04490"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HPC11340.1"
                     /translation="MAANIVVVNIYGEDYPITGVTDAAHISRVADFVNARVQQIAQSS
                     RVKARDKVAILAAMQIASELLEKSEQLESTLGQVEGNLDSLLDRLDRELAPVSDSR"
     gene            complement(1245..1496)
                     /locus_tag="PK112_04495"
     CDS             complement(1245..1496)
                     /locus_tag="PK112_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11341.1"
                     /translation="MNLEEKFQKLLEQVGGLTRQSQTLRKENSDLSRENDSLKRELAG
                     LRAECHRLKLQAADRADLVTSKLSSVLQRLDELERTEGQ"
     gene            complement(1493..>2753)
                     /locus_tag="PK112_04500"
     CDS             complement(1493..>2753)
                     /locus_tag="PK112_04500"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF036799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HPC11342.1"
                     /translation="RAAALLADLGGGEVLAGMVDAYPRRIDPKAIALRPRRCNDVLGT
                     DLTAAQMKEILEGLEFAVEDGDPLRVTVPTFRPDVEREIDLIEEVVRILGFDRVEDAR
                     STIGPLFTPLHFEDTFKDQVRQVLTGAGFDEVLSHGLADSHLADLAAPELPQLRLVST
                     ASAELNIMRNSMILSAVPILGHNVARRNVDLRIFEIGKVYFPPTPAGEWIEDERILLA
                     VTGDTPHTWREKPRPHDFYDVKAGLERLAEHFHWPALEFVPQAVSFLEPGASFRLRVR
                     ETEIGAIGQLSVKLARQADIKQAVWLAEVATGPLLPLSSRVAAYEPLPIYPAAPRDLA
                     LVVDETVPAGDIVGAIRTAAGELAEDVSIFDLYTGKQIEAGKKSIAVAISYRSKERSL
                     SSEEVEAAQQKVVAAVKKLFNAEVRDK"
BASE COUNT          444 a          896 c          818 g          595 t
ORIGIN      
        1 cgtcgattcc ggtggcggtt tcgaaggagc ggatgttgcg tccctcgcgg ccgatgatcc
       61 gccccttcat ctcgtcgttg gggaggttga cgacggagac ggtggtttcg acggtgtggt
      121 cggcggcgca gcggtagatg gaggagagga tgatttcgcg ggcttctttt tcggcgtcgg
      181 cttcggcctt ctcgcggatt tccttgatgt attgggccgc ctccatgcgc gcggcgttga
      241 tcatgttgtc catcaactgc ttcttggctt cttcgggcgt catctgcgcg attttctgga
      301 ggcgttcatt ttgtcgggcg atgatctggt cgagttcttc ctcccgcaga gcgatcccct
      361 tttcccgcgc ctgggtggct ttttcgcggt tgacgagatc tttttctttg gcgtcgaggt
      421 gctcggtctt gcgtcccagg ttggtgtcga gttcggagag ccgtttgtcg gctgcgtcga
      481 gttcctgccg tcgggcggcc acctcccgtt caaagtcccc tttgagcttg aggaactcct
      541 cgcgggcctc gaggcgggcc tctttccgcc ggatttcggc ttcctttttg gcctcctcga
      601 ccacgcgctc cgcctcgacc gtggccgctg cgagcttctc gcgattcatc cgctgggccg
      661 cacgccagga gaggaagtat ccgatgacgc cggcgacaac cgcggcggcg gcaacgatga
      721 tgattttatc cattgcgtcc tccaccccgg caccgcgtgc cggtggactg atgcatgtat
      781 ctcaaaaagg gagtaagaag agaacggcgc ggatcggagt ttcgctgatc cactcaacga
      841 ctgtcggaaa ccggcgccag ctcacgatcg aggcgatcca gcagggaatc gaggttgccc
      901 tcgacctgtc ccagggtcga ttccagttgc tctgatttct ccagcagctc cgatgcgatt
      961 tgcatggctg ccaggatcgc aactttgtcg cgagccttca cccggctgct ctgcgcgatt
     1021 tgctgcaccc gcgcattgac gaaatctgca accctggaaa tatgagcggc atcagtcaca
     1081 cccgtgatcg ggtagtcctc gccgtatata ttcaccacga caatgttcgc tgccatactt
     1141 tcaaacgccg ttgtgggcgg tttgtcagat cgcgcttgtt ctctcttatc ccgcttacag
     1201 tcaacgattt ataattcgag cggagaatcc tgtcaagaaa aaatttactg cccctcggtg
     1261 cgctccaatt cgtccagccg ctgtaacacg ctggacagct tggaggtaac cagatccgcc
     1321 cggtcggcgg cctggagttt taaccggtgg cactccgccc gtaacccggc cagctcccgc
     1381 ttcaatgaat cgttctcccg gctcaggtcg gagttctctt tccgaagggt ctggctttgc
     1441 ctggtgaggc cgccgacctg ctccagcaat ttctgaaatt tctcctcaag gttcacttgt
     1501 cccgcacctc tgcgttaaac agtttcttca ccgccgcgac caccttctgc tgcgccgctt
     1561 caacttcctc gctggacaag ctgcgttctt tggagcggta ggagatcgcc acggcgatcg
     1621 atttttttcc ggcctcgatt tgcttgcccg tatagagatc gaagatactc acgtcttcgg
     1681 ccagttcccc ggccgccgtg cggatcgcac cgacaatatc gccggccggc accgtttcgt
     1741 caacgaccag ggcgaggtcg cgcggggcgg ccggataaat cggcagcggc tcgtacgccg
     1801 ccacccgcga ggaaagcggc agaagcggcc cggtcgccac ctccgccagc cataccgcct
     1861 gcttgatatc cgcctgccgg gccagcttca ccgacaactg cccgatggcc ccgatctcgg
     1921 tttcccgcac ccgcagccgg aacgaagctc ccggctccag gaacgatacc gcctgcggga
     1981 cgaactccag cgccggccag tggaagtgtt ccgccagcct ctccaacccg gccttcacgt
     2041 cgtagaagtc gtgcgggcgc ggcttctccc gccaggtgtg cggggtatcg ccggtcactg
     2101 ccagcaggat ccgctcgtcc tcgatccact cgccggccgg ggtcggcggg aagtacactt
     2161 tgccgatctc aaaaatccgc aggtcgacat tgcgccgggc cacgttatgg ccgaggatgg
     2221 ggacagccga gaggatcata ctgttgcgca tgatgttcaa ttccgcactg gccgtgctga
     2281 ccagccgcaa ctgcggaagc tccggcgccg cgaggtcggc caggtggctg tcggcgagac
     2341 cgtggctgag cacctcgtcg aatcccgccc ccgtcagcac ctgccgcacc tggtctttga
     2401 aggtgtcctc gaaatgcagc ggcgtgaaca gcggaccgat cgtcgaccgg gcgtcttcca
     2461 cccggtcgaa cccgagaatc ctcacgactt cttcgatcag atcaatctcg cgctcgacat
     2521 ccggccgaaa ggtcggcacc gtgacccgca acgggtcgcc gtcctcgacg gcgaactcca
     2581 gcccctccag gatctccttc atctgtgcgg cggtcaggtc ggtcccgagc acgtcgttgc
     2641 accggcgggg gcgcagggcg atggctttcg ggtcgatccg ccgggggtag gcatcgacca
     2701 tcccggcgag cacctcgccg ccgcccaagt cggccaggag agccgccgcc cgg
//
LOCUS       DAOOKS010000223         2753 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_45112_length_2753_cov_2.991846, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000223 DAOOKS010000000
VERSION     DAOOKS010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45112_length_2753_cov_2.991846"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1695
                     /locus_tag="PK112_04505"
     CDS             <1..1695
                     /locus_tag="PK112_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11343.1"
                     /translation="VASSGGSPVWVETHDDVVISYGVFEVQLGETSPLTRSHFDGSPR
                     WLSIAVDGGAESAVRLPVNSSAYTIRANYADTSGRAKYADTAAFAVGVGNSGWADDGA
                     NVRLVTASDWLGIGTASPEDKVHIESSLSGGTAFLKLETSHASNWGETGLRIETPQNR
                     WHLRMDDATNNNIPDGALGLRSQDVGTEIMTWTSDGKVGILNTLPEYPLDVRSTAVAI
                     YGRTTGGLGTAGVYGRAENDGFGVMGTSTSGRGVYGASASGFGGYFLGPTTYVSGNLG
                     VGTETPTHKLTLNGTLAYQSAGTTKYHVAYYGGGLDFCETGVGDYRLMIEDGGEVGIG
                     TANPTAKLHVMGNVKVRDTLTAGAIFSSSIVDEFGLASAKNALYESTACTGSWKSCLS
                     RTIRVPAPGWVLAVATARVKLDHPGSGSTDVSVALSDDSTTADDWWYANWALPGAAVA
                     GNYYSTLNPMRLFRTDTAGVYTFYVNARRLDGEASVLGAQMHCFFIATSYSFDEAVAG
                     GEGSGAQPAAPVLTELGDVPAPAENPGAADVASILAELSALRQEVESLRNKVEANR"
     gene            complement(1780..2214)
                     /locus_tag="PK112_04510"
     CDS             complement(1780..2214)
                     /locus_tag="PK112_04510"
                     /inference="COORDINATES: protein motif:HMM:NF024644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPC11344.1"
                     /translation="MNSRLVVRPNLCIGCRTCELACSFTHSADGKPGRSRIFMLDGGH
                     EKLWVTVTCLQCDDPACVKSCLVGALRRNEQTGAIELNQETCVKCMACVAACPFGCAI
                     LDHTHDRVVKCDLCGGDPACARFCPTKALDYRPIAQAAKKAG"
     gene            complement(2211..>2753)
                     /locus_tag="PK112_04515"
     CDS             complement(2211..>2753)
                     /locus_tag="PK112_04515"
                     /inference="COORDINATES: protein motif:HMM:NF026048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPC11345.1"
                     /translation="HSDTVIELRRTLLDLQLARHPRTPLIQQMAAEYGVIKTSYEEAP
                     DGDDCILCALCTRVCDAMGFRAISTVGRGHGKRVAPPLDQPPPDCVGCLACAQICPTG
                     FITYKEFGAGREIWGKQFQMVTCTRCGAPTITREFADNLAARREIPREYFDVCDACHR
                     AELAGTMGRIAQWNREEAAR"
BASE COUNT          469 a          918 c          915 g          451 t
ORIGIN      
        1 gtcgcgagct ccggcggctc ccccgtatgg gtggagacgc acgacgacgt agtcatttcg
       61 tacggcgtct tcgaggtgca actcggagag acctccccgc taacccgctc gcacttcgac
      121 ggttcgcccc gctggttgtc gattgccgtg gacgggggag cggaatcggc tgtccgtttg
      181 ccggtgaaca gctcggccta tacgatccga gccaactacg ccgacacctc gggccgggcg
      241 aaatacgccg acaccgccgc tttcgcagta ggcgtcggga actccggctg ggcggacgac
      301 ggagcaaacg tgcgactggt gaccgccagc gactggctcg gcatcggcac ggccagtccg
      361 gaagacaagg tgcatatcga gagcagcctg agcgggggaa cggccttcct gaaacttgag
      421 acctcgcatg cctctaactg gggggagacg ggcctgcgca tcgaaacgcc gcagaaccgc
      481 tggcaccttc gcatggacga cgccacgaac aacaacattc ccgacggcgc cctcgggctg
      541 cggagccagg acgtcggcac cgaaatcatg acctggacga gcgacggcaa ggtggggatt
      601 ctgaacacgc tgccggagta tcccctggat gtgcgctcga ccgcggtcgc gatttacggt
      661 cgcacgaccg ggggcctcgg gacggccggc gtgtacggac gggcggagaa cgacggattc
      721 ggggtgatgg ggacgtcgac gagcggccgg ggcgtctacg gggcgagcgc gagcggtttc
      781 ggcggttatt tcctgggccc gaccacctac gtctccggca acctcggcgt cgggaccgag
      841 acgcccaccc acaagttgac cctcaacggc accctcgctt atcagagcgc cgggacgacc
      901 aagtatcacg tcgcctacta cggcggcggc ctcgatttct gtgaaacggg cgtcggggat
      961 taccgcctca tgatcgagga cgggggagag gtggggatcg gaaccgccaa cccgaccgcc
     1021 aaactgcacg tcatggggaa cgtcaaggtg cgggacaccc tcaccgcggg cgccatcttc
     1081 tccagcagca ttgtcgatga gttcggcttg gcgtcggcca aaaacgccct ttacgaatcg
     1141 acggcctgca ccggctcgtg gaaatcatgt ctgtcccgga ccattcgcgt gcccgcgccc
     1201 ggctgggtcc tggctgtcgc gacggccaga gtgaagctgg atcatccggg ctcaggttcc
     1261 actgatgtca gcgtcgctct gtcggatgat tcgacaacgg ccgacgactg gtggtatgcc
     1321 aactgggcgc tccccggcgc ggccgtggcc gggaactact actcgactct caaccccatg
     1381 cggctattcc gaaccgatac cgccggtgtg tataccttct acgtgaacgc aaggaggttg
     1441 gacggggaag cttctgttct gggcgcccaa atgcactgct tcttcatcgc caccagctac
     1501 agcttcgatg aggctgttgc gggcggcgag gggagcggcg cgcagccggc tgctcccgta
     1561 ttgaccgagc tcggggacgt tccggcgccg gccgaaaacc ccggggctgc agacgtcgca
     1621 tcaatcctcg ccgagctctc cgccctccgg caggaagtgg agtcgctcag gaacaaggtc
     1681 gaggcgaacc gatagaacgg gttcactccg atattgcccc aaaacgagag gcgtcggccg
     1741 tttccggccg acgcctccgg cttttcctta gggccgggtt cagccggctt ttttggcggc
     1801 ttgcgcgatc ggccggtaat ccagcgcttt ggtcgggcag aaccgggcgc aggccgggtc
     1861 cccgccgcac aggtcgcact tgaccacccg gtcgtgggtg tgatcgagga tcgcgcaccc
     1921 gaacgggcag gccgccacgc aggccatgca cttgacgcac gtctcctgat tcagctcgat
     1981 cgccccggtc tgttcgttgc gccggagcgc gcccacgagg caggacttga cgcacgcggg
     2041 gtcgtcgcac tggaggcacg tgaccgtcac ccagagcttc tcgtgcccgc cgtcgagcat
     2101 gaatatccgc gaccgccccg gcttgccgtc cgccgaatgg gtgaacgagc aggccagctc
     2161 gcaggttcgg cagccgatgc agagattcgg ccgcaccacc aaccgggaat tcatcgcgcc
     2221 gcctcctccc ggttccactg ggcgatccgc cccatggtgc cggccagctc cgcgcggtgg
     2281 caggcgtcgc acacgtcgaa gtactcccgg gggatctccc gccgcgcggc gaggttgtcg
     2341 gcgaattcgc gggtgatggt cggcgcgccg cagcgcgtgc acgtgaccat ctggaactgc
     2401 ttgccccaga tttcccgccc ggcgccgaat tccttgtacg tgataaaccc ggtcgggcag
     2461 atctgggcgc aggccaggca gccgacgcaa tccggcggcg gctggtcaag cggcggcgcc
     2521 acgcgcttgc cgtgcccgcg ccccaccgtc gatatggccc ggaaacccat cgcgtcgcac
     2581 acccgcgtgc acagcgcgca caaaatgcag tcgtccccgt ccggcgcctc ctcgtagctc
     2641 gtcttgataa ccccgtactc ggccgccatc tgctggatga gcggcgtgcg ggggtgccgc
     2701 gcgagctgga gatcgagcag cgtccggcgc agttcgatca ccgtgtcgga gtg
//
LOCUS       DAOOKS010000224         2753 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_45114_length_2753_cov_2.925871, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000224 DAOOKS010000000
VERSION     DAOOKS010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45114_length_2753_cov_2.925871"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..2333)
                     /locus_tag="PK112_04520"
     CDS             complement(132..2333)
                     /locus_tag="PK112_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010855117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4954 family protein"
                     /protein_id="HPC11346.1"
                     /translation="MDNVSILPPGSIGRQFIPPEFLPPGQDEYHFRNIQAPHPAGRFR
                     HLRSDEVERLVKNSNDCDNWDQLLVTDEFDPGQIKNSKFFGLVRIGRLRNMILEHHDF
                     RVPTGITNSSIVSCDIGDHVAIHGVRYLAYYIIGDRCILTNIDEMNTSNHAKFGNGIL
                     KEGEPEKIRVWLDLMNETGCRQVLPFDGMIPADAYLWAKYRDDRPLLEQLKQITQNSF
                     DHRRGYYGMVADQCVIKNSRILKDVKIGPHCYIKGANKLKNLTIHSSPDEPTQIGEGV
                     ELVNGIIGCGCHIFYGCKAVRFILGQNSNLKYGARLIHSFLGDNSTVSCCEILNNLVF
                     PAHEQHHNNSFLVASLVLGQSNMAAGATIGSNHNSRANDNEIQAGRGFWPGLCTSVKH
                     SCRFASFTLLSKADYPAELNIPLPFSLLSNNASRDQLEVTPAFWWLYNMYALIRNSWK
                     FADRDKRLNKIQNIEFDFLAPDTVEEILSARQLLQRWTAQALGRIDPQIQAPTGEECT
                     RLGEKLLRESDPRLKTLEVLGENMEKSRRKVVIQKCQPAYRAYEDMLYYYAVNNLIAW
                     LQAHPGAKFHDLSAALKNQRQTQWVNLGGQLIPAPDVDQLRSDIRTGKLSAWPDIHNR
                     YEDLWKNYPLEKQKHAWATLTLLLDVAEPSLPQWHSALDRSLTIQQYICDQVYISRKK
                     DYDNPFRQATYRNQEEMIAALGTIDDNSFVKQVRQETEQYHRTIGEIRQRG"
BASE COUNT          492 a          575 c         1043 g          643 t
ORIGIN      
        1 gggcctggtt gaggtcgaaa tttttcacga tatcgatggt gtccagcatt tcgcggacga
       61 gggcgtactt aaaataattt tcctgacgat tcatgggttg ctccttgatt aaaggttttt
      121 gtttttcggt tttagccgcg ctgccggatt tctccgatgg tgcggtggta ttgttcggtt
      181 tcctggcgta cttgtttgac gaagctgttg tcgtcgatgg tgccgagggc ggcgatcatt
      241 tcctcctgat tgcggtaggt ggcctggcgg aagggattgt cgtaatcttt cttccgcgag
      301 atatagacct gatcgcagat gtattgctgg atggtgagcg agcggtccag ggctgaatgc
      361 cactggggca gggagggttc cgcgacatcc agcaggaggg tgagggtggc ccaggcgtgt
      421 ttttgttttt cgagcgggta gtttttccag aggtcttcgt accggttatg gatgtcgggc
      481 caggcggaga gcttgccggt gcggatatcg gagcggagct ggtccacgtc gggtgcgggg
      541 atgagctggc cgcccagatt gacccattgg gtttggcgct gatttttcag ggcggcgctg
      601 agatcgtgga atttggcgcc gggatgggct tgcagccagg ctatcagatt gttgacggca
      661 tagtagtaga gcatgtcctc gtaggcgcga tacgccggct ggcatttctg gatgaccacc
      721 ttgcggcgag atttttccat gttttcgccc agcacttcca gggtttttag ccgcgggtcg
      781 gactcgcgga ggagtttttc gccgagccgg gtgcattctt cgccggtcgg ggcctggatt
      841 tgcgggtcga tgcggcccag ggcctgcgcg gtccagcgct ggagcagttg gcgggcggag
      901 aggatttctt cgacggtgtc gggggccagg aagtcaaatt cgatgttctg gatcttgttc
      961 aggcgtttgt cgcggtcggc gaatttccag gaattgcgga tgagggcgta catgttgtag
     1021 agccaccaga aggcgggggt gacttccaac tggtcgcggg aggcgttgtt gctgagcagg
     1081 gagaagggca gggggatatt cagctcggct gggtagtccg ccttggagag caaggtgaag
     1141 gaggcgaagc ggcaggagtg cttgacggag gtgcacaggc cgggccagaa gccgcggccg
     1201 gcctggattt cgttgtcgtt ggcgcggctg ttgtggttgg agccgatggt ggcgccggcg
     1261 gccatgttgc tctggcccag gaccagggag gcgaccagaa aggaattgtt gtggtgctgt
     1321 tcgtgggcgg ggaagaccag gttgttcagg atttcgcagc aggagacggt ggaattgtcg
     1381 ccgaggaagg agtggatgag gcgggcgccg tatttgaggt tggagttctg gccgaggatg
     1441 aagcggacgg ccttgcagcc gtagaagatg tggcagccgc agccgatgat gccgttgacc
     1501 agttcaacgc cttcgccgat ctgggtgggt tcatcggggg aggaatggat ggtcaggttt
     1561 ttgagcttgt tggcgccttt gatgtagcag tgggggccga ttttgacgtc cttgaggatg
     1621 cgggagtttt tgatgacgca ctgatcggcg accatgccgt agtagccgcg gcggtggtcg
     1681 aaggaattct gggtgatctg cttgagctgc tccaggagcg ggcggtcgtc gcggtatttg
     1741 gcccagagat aggcgtcggc gggaatcatg ccgtcaaagg ggaggacctg gcggcagccg
     1801 gtttcgttca tgaggtccag ccagacgcgg attttttccg gttcgccctc cttgaggatg
     1861 ccgttgccga atttggcgtg gttggaggtg ttcatttcgt cgatgttggt caggatgcag
     1921 cggtcgccga tgatgtagta ggccaggtag cggacgccgt ggatggcgac gtggtcgccg
     1981 atgtcgcagg agacaatcga gctgttggtg atgccggtgg ggacgcggaa gtcatggtgt
     2041 tcgagaatca tgttgcggag gcggccgatg cggaccagac cgaagaattt cgagttttta
     2101 atctggccgg gatcgaattc gtcggtgacc aggagctggt cccagttgtc gcagtcgttg
     2161 ctgtttttca ccaggcgctc gacctcgtcg gagcggaggt ggcggaagcg gccggcgggg
     2221 tggggggcct ggatgttgcg aaagtgatat tcatcctggc cgggggggag aaattccggg
     2281 gggatgaact ggcggccgat gctgccgggc ggcaaaattg aaacgttatc catgttcgaa
     2341 ctcctacctg ctatgagcta ctttttcggt tccgcgaatc caggaccgat tgtagttggc
     2401 atggcaatac tataaaagaa aaaaatggga ctgtacacat gaaaaaaaaa gaggggggcg
     2461 gcggggaggc gggcagatgg aaccacggga aactttgcgg ttgattgcgg ggagtggagg
     2521 gcatacaatg ccgcgattat ggtaaaaagg cgaaaattga gaacaaggaa gcgcagttgt
     2581 gagtgaagaa acggtaaaga ttgagaagct gggtaatggc atgacgctgg tggcggagcc
     2641 gatgggggag gtatcgtcgg gtgcgtttgt gatactggtg ccggcggggg cggcgtatga
     2701 tccggcgggg cggacgggga cggcgggcgt gctgggggat ctggtgtttc ggg
//
LOCUS       DAOOKS010000225         2752 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_45128_length_2752_cov_3.536893, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000225 DAOOKS010000000
VERSION     DAOOKS010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45128_length_2752_cov_3.536893"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="PK112_04525"
     CDS             complement(<1..303)
                     /locus_tag="PK112_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11347.1"
                     /translation="MAGSAGPSVRARLSELAAAHPLATLLGTAALLRLLAAVFARGYM
                     ASDDHHQVVEIAARWLRGIPMFLDGQEVFYRSLLYPAVHWLILRAARAVGVVDPDAA"
     gene            829..2403
                     /locus_tag="PK112_04530"
     CDS             829..2403
                     /locus_tag="PK112_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010881359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HPC11348.1"
                     /translation="MARERWGTKIGLILAMAGNAVGLGNFLRFPVQAAENGGGAFMIP
                     YFVALVLLGIPLMWVEWGMGRYGGRFGHGATPGQFHAMWQHPISKYLGALGLFLSLAT
                     MIFYVYVESWSLGFSFFSITKSYFGMESFDSMRHFLSSYQGVTEGFFTSIWPAYLILL
                     LTLAINFYVVYHGIAKGIERLALIAMPALILFAIILVVRVVTLGTPDPVAHPDWNVES
                     GFAFIWNPDLSQLGSAKVWLAAAGQIFFTLSLGSGLIQTYSSYVRENDDIALGGLTTA
                     SINEGVEVVLGASIAIPIAAAFFGIAVTQEIAARGAYDLGFVSLPIIFTKIPAGSIFG
                     CLWFLLLFFAGITSSVAMAQPLIAFLKEEFRFSHRKAVAVLAALVLIPIHFVVFFLGK
                     GYLDEMDYWVGTFGLVVFALIETIVFAWVFGMDKGWAEITRGAEVRVPRIFYYVIKYV
                     TPVYLLVIMIYWIVLDAVPTLLMRGGNPENVPYRWLSRAVMLLLFVVMIYLVRRAWRR
                     NDVVKSFERPDEEAAV"
BASE COUNT          405 a          935 c          888 g          524 t
ORIGIN      
        1 ggccgcgtcg ggatcgacga cgccgaccgc gcgggccgcg cgcaggatca gccaatgcac
       61 cgccgggtag agaagggatc ggtagaagac ctcttgccca tcgaggaaca tggggatccc
      121 gcggagccat cgggcggcga tctccaccac ctgatggtgg tcgtccgacg ccatgtaccc
      181 ccgcgcgaag accgccgcga gcagacgcag aagcgcggcg gtgcccagga gtgtggccag
      241 cgggtgggcc gcggccagct cggagagccg cgcgcggacg gaggggcccg ctgaccccgc
      301 caccggggcc gcgtccgccg tgcgtcccgc cgggattcgg gccgtcctat ccgccgcgcc
      361 tggcgccggg gcctccgccg ggcactcccg atcgcggcca gtctcctcca tcgtcctcct
      421 cggtggtggg tgggccgtcc gtggtcctct gccgtcgcgg tcggattaca gcccgagggt
      481 gcgcgggccg caaggtccga tcgctccgcc ggcggcggac ggacggggat ccgaaaaccg
      541 cctgaaccgg gaccaagaca gatcgcgtgc ggcgtccgcc ccggggcgcc cggagtcctc
      601 agggtgcgcg gcgcggcgcc aaagcgcccg cgcggcaacc cgggcggcca cttcgtgctt
      661 gcgcgccccc tcccccccat gatatatagt cgcattccgg cctgaggggc cggatgcctt
      721 cgtaagtcag gcggttgccg ctgcaccgcc ccacatcttt cacagccaga tcacacgcac
      781 ctttcacgca cttcggttcc accgcgcggg accgatggag gtagagcgat ggcacgagag
      841 cgctggggaa ccaaaatcgg actcatcctg gccatggccg gtaacgcggt cggcctgggc
      901 aatttcctgc gtttccccgt ccaggcggcg gagaacggcg ggggggcgtt catgatcccc
      961 tacttcgtcg ccctcgtcct gctgggcatt cccctcatgt gggtggagtg gggcatgggg
     1021 cgctacggcg gccggttcgg gcacggcgcg acccccggcc agttccacgc catgtggcag
     1081 cacccgatct ccaagtacct cggcgccctc ggcctgttcc tctcgctggc cacgatgatc
     1141 ttctacgtgt acgtcgaatc gtggtcgctc gggttcagtt tcttctcgat caccaagtcg
     1201 tacttcggca tggagtcgtt cgactcgatg cgccacttcc tgtcgagcta ccaaggggtg
     1261 acggaagggt tctttacgag catctggccc gcctacctga tccttctgct caccctggcg
     1321 atcaacttct acgtcgtcta ccacgggatc gccaagggga tcgagcggct ggccttgatc
     1381 gcaatgccgg ccctgattct cttcgcgatc atcctggtcg tccgcgtcgt cactctcggg
     1441 acacccgacc cggtggcgca cccggactgg aacgtcgaat ccggcttcgc ctttatctgg
     1501 aatcccgatc tctcgcagct cggctcggcc aaggtctggc tcgccgccgc cgggcagatt
     1561 ttcttcaccc tgagcctcgg ctcggggctc atccagacgt actcgagcta tgtgcgcgag
     1621 aatgacgaca tcgcgctcgg cggtctcacg accgcgtcca tcaacgaagg ggtggaggtg
     1681 gtgctcggcg cgagcatcgc catccccatt gcggccgcct tcttcggcat cgccgtcacc
     1741 caggagatcg cggcccgggg cgcctacgat ctcggtttcg tctcgctgcc gatcatcttc
     1801 accaaaatcc cggccggatc gatcttcggc tgcctctggt tcctcctgct cttctttgcc
     1861 ggcatcacct cgtcggtggc gatggcccag ccgctcatcg ccttcctcaa ggaggaattc
     1921 cgattcagtc accgcaaggc ggtggcggtg ctggccgccc tcgtgctcat cccgattcac
     1981 ttcgtcgtct tcttcctcgg caagggctac ctggacgaga tggactattg ggtggggacg
     2041 ttcggactgg tcgtgttcgc cctcatcgag acgatcgtat tcgcctgggt tttcgggatg
     2101 gacaaaggct gggcggaaat cacgcgcggc gcggaggtcc gcgttccccg catcttctac
     2161 tacgtgatca agtacgtcac gcccgtctac ctgctggtca tcatgatata ctggatcgtc
     2221 ctcgacgccg tcccgaccct gctcatgcgc ggcggcaatc cggagaacgt cccctaccgg
     2281 tggctgtccc gcgcggtcat gctgctgctg tttgtcgtga tgatctatct cgtgcgccgg
     2341 gcctggcggc ggaacgacgt ggtcaaatcc tttgaacggc ctgatgagga ggccgcggta
     2401 tgaacctcac cgtcggcgga ataatcttcc tcgcggtcgc ctggggcatc atcctgtccc
     2461 tgtcggcctt ttgcctggcg cgggtgttga ggaagaaggc gggctcgccg gactagcgtt
     2521 tttttcggcc gtccgcgtca acgttttcgc cgggtgcggt gtcgttaccg gcagaaacct
     2581 gaaaaccgga ggatcccatg acgacaccaa tcctgagacg cctgctgccc gcggcggccg
     2641 cggcgctggc cctgggctgc agcgcctgcc tggagcggaa agagacgatc acggtcgggc
     2701 cggacggttc ggccgtgtgc gagatagaat tcacggggga cgcccaggtc tt
//
LOCUS       DAOOKS010000226         2741 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_45366_length_2741_cov_3.709233, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000226 DAOOKS010000000
VERSION     DAOOKS010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45366_length_2741_cov_3.709233"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2486)
                     /locus_tag="PK112_04535"
     CDS             complement(<1..2486)
                     /locus_tag="PK112_04535"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPC11349.1"
                     /translation="MSVLRLEELAPYGIPRRLIELWRERQGETLLPVQSRAVRRGLLG
                     APGERAEEAAPLRMLISAPTAAGKSFCAEMAVARTLAGRRKALVLVPLRSLADQKHRL
                     LAGTFGALGLRCLIASGDYPENDRAFAESDYDVAVAIFEKFDGLLGTRLEVLRAIGLL
                     VVDEIQTVSEPGRGAVLERLLTCVRASAYRPGLLGLSAVIGEGEKAAGRLAEWLEAGL
                     VVESSRPVDLLRGVAAEGAFRYRSFNSGREASEPFVLAREPGGDLFESLIERLREEPG
                     STIVFLKSRRETVEAAMRLAGAVGWPAAEQGTAELAEEEPSALNRILRRVMARGVAFH
                     NADLSPRQRRAVEESFARREVRVIFATTTLAMGVDLPADTVYLETVKYTGGRRDARPA
                     LVPVSRAEFDNMAGRAGRAARAEVRPGRAVALAQSEFEGDILWENYIASGEYTSVRSA
                     FDSMPLADWALHVLATGLAADAAGLAAVYRRSLRAVTEPEAPPPDFEAVLDLLTREGV
                     AGREPDGLVRPTPDGETAVQTGLTCAQAVHYTRRLRESRPAEEFGWLALALSGPDWEL
                     PAGLLSRQELADNGPLRRLYRRCDHWLASARVLIGQPGGRGLTEYRTAGALKAALLLE
                     QWRRMTPVRELEEEFRVHAGQIMAIGDTAAHLLSGIAGLLDNLDGGGDKLRPLVFSVR
                     FGVVPELQGLHAAFRDILARSDFAALAQAGIVRVPDLIGHDPTDLGEIVKQERKLQKL
                     LARIMEWKKEVSMRQSEVLSRVAPYPVRGSGPRPASVEIDGSCERERYLVRVDGRPVW
                     LTGKSFKYFTRLAWSRVQRDGGWMYKEDIE"
BASE COUNT          441 a         1129 c          777 g          394 t
ORIGIN      
        1 tgctcaatat cctctttgta catccacccc ccgtcccgct gcacacgcga ccaggccagc
       61 cgcgtgaagt acttgaacga tttccccgtc agccacaccg gccggccgtc cacccgcacc
      121 aaataccgct cccgctcaca tgatccgtcg atctccaccg acgccggccg cggccccgac
      181 ccgcgcaccg gatagggtgc cacccgcgaa agaacctccg actgccgcat acttacctcc
      241 ttcttccact ccattatcct ggccagcaat ttctgcagtt tgcgctcctg cttcacgatc
      301 tcccccaggt cggtcgggtc gtgtccgatc aggtcgggca cccggacaat tcccgcctgc
      361 gccagcgccg caaaatcgga gcgcgccagg atatcgcgga aggctgcgtg cagcccctgc
      421 aactccggga cgactccgaa gcgaacgctg aacacgagcg ggcggagttt gtctccgccg
      481 ccgtcaagat tatcgagcaa cccggcaatg ccgctcagca ggtgcgcggc tgtatcgccg
      541 atcgccatga tctgccccgc gtggacccgg aattcctcct ccagctccct gaccggcgtc
      601 atgcgccgcc actgctccag cagcagcgcc gccttgagcg cgccggccgt gcggtactcc
      661 gtcagccctc gtccccccgg ctggccgatc agcacgcggg cggatgcgag ccaatggtca
      721 cagcggcggt acaaccgccg cagcgggccg ttgtccgcca gttcctgccg actgagcagt
      781 cccgcgggca actcccagtc gggcccgctg agcgccagcg ccagccaccc gaattcctcg
      841 gccggccgac tctcccgcag ccgccgggtg taatgcaccg cctgcgcgca ggtcagcccg
      901 gtctgcacgg cggtctcccc gtccggggtc ggacgcacca ggccgtccgg ttcgcgcccg
      961 gccactccct cccgcgtcag caagtccaga accgcctcga aatcgggggg cggcgcctcc
     1021 ggctccgtca ccgcccgcag cgagcgccgg tagacggcgg ccagtcccgc cgcatcggcc
     1081 gccaggccgg tcgccagcac gtgcagcgcc cagtccgcga gcggcatgga gtcgaacgcc
     1141 gaccgcaccg atgtgtattc tccggaggcg atgtagtttt cccagaggat atcgccctca
     1201 aattccgact gggccagggc caccgcgcgg cccggccgca cttccgcccg ggccgctctc
     1261 ccggcccgcc cggccatgtt gtcgaactcc gcccggctca ccggcacgag cgcgggccgg
     1321 gcatcgcgcc gccccccggt gtacttgacc gtctcgagat acaccgtgtc ggccggcagg
     1381 tccaccccca tggccagcgt cgttgtggcg aagatcaccc gcacctcccg ccgggcaaaa
     1441 ctctcctcca ccgcccggcg ctgccggggg gagagatccg cgttgtggaa cgccaccccg
     1501 cgcgccatga cccggcgaag aatgcggttc aacgccgagg gctcctcttc cgccaactcc
     1561 gccgttccct gctcggccgc cggccagccg accgcccccg ccagccgcat ggccgcctcg
     1621 accgtctccc gccgcgactt gagaaagaca atcgtcgatc ccggttcctc gcgcagccgc
     1681 tcgatcaggc tctcgaacag atcgccgccc ggctcgcgcg caaggacaaa cggctcgctc
     1741 gcctcccgcc cggaattgaa cgagcggtag cggaacgccc cttcggcggc gaccccgcgg
     1801 aggagatcga ccggacggct gctctcgacc accagcccgg cctccagcca ctcggccagc
     1861 cgcccggccg ctttctcccc ttcgccgatc accgcagaca agccgagcag tcccggccgg
     1921 tatgccgatg cccgcacgca cgtgagcagc cgctcgagca cagcgccgcg ccccggctcc
     1981 gacaccgtct ggatttcgtc cacgaccagg agcccgatcg cgcgcaggac ttcgagacgg
     2041 gtgccgagca gaccatcgaa tttctcgaaa atcgccaccg cgacgtcgta atccgattcc
     2101 gcaaaagcac ggtcgttttc gggataatcg ccgctcgcaa tcagacaccg caggccgagc
     2161 gccccgaacg tccccgccag caggcggtgt ttctggtcgg ccagcgagcg aagcggcacg
     2221 agcacgagcg ctttccgccg ccccgccagt gtccgggcaa ccgccatctc ggcacaaaac
     2281 gatttccccg ccgccgtcgg cgcgctgatc aacatgcgca gcggcgccgc ttcctcggcc
     2341 cgttcccccg gcgcgcccag cagaccccga cggaccgccc ggctctgcac cggcagcagc
     2401 gtctccccct gccgctcccg ccagagctca atcagccggc ggggaatgcc atatggcgcc
     2461 aactcctcca accgtaacac actcatctgt cctcccgctt ccggccgcac tccgccggcc
     2521 actcagcatc tcagtgtccc tcttcgcctc gccctgcacc gcccgcaacc gtccgcccgt
     2581 cgtcagttgc acccgcccca cccgcacatg gggcaggtga aacaagcccc cgcgcgaatc
     2641 atcccgctgc tgcagtccgg acaggtggcg gcatcgaggt aacagcaatc gccacccccc
     2701 gccaccgccg tgttcatcca gtcaaatgaa ctctccgccc c
//
LOCUS       DAOOKS010000227         2734 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_45536_length_2734_cov_3.274356, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000227 DAOOKS010000000
VERSION     DAOOKS010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45536_length_2734_cov_3.274356"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="PK112_04540"
     CDS             complement(<1..354)
                     /locus_tag="PK112_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11350.1"
                     /translation="MNQAQIEKTAKIRMTGVGGQVGAGAEHVEPLDVLADLEATLDEV
                     AEANPRLTSEETHTSKSVEDLKALLEASLAINSSLVTDDVLQIVMRKAIELMQAERGL
                     VMLLDDANQLQVRSAY"
     gene            complement(453..1106)
                     /locus_tag="PK112_04545"
     CDS             complement(453..1106)
                     /locus_tag="PK112_04545"
                     /inference="COORDINATES: protein motif:HMM:NF025692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HPC11351.1"
                     /translation="MRFFRPQARRRSPAALLALILAAAAPARGADPPGGVEYDIVRAD
                     DSLAVWLNLTPFFGHPEYRSLQDGIDYACEIAIELQRPRKLFGAVPVTKNSCTIRLSY
                     RLLTEAFRVQTSGPGQSAKERLLLSREEAEGFLADSVVVRVAALAGLPADRDYRITLD
                     ISRIALTGINLAVDDDTGSVSPVKTLFREFLRLTNYGREEWSTTSRSFSLSEVLPRR"
     gene            complement(1107..>2734)
                     /locus_tag="PK112_04550"
     CDS             complement(1107..>2734)
                     /locus_tag="PK112_04550"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HPC11352.1"
                     /translation="FRVAFTLDEISVAAAADSLPAEASVPRAAVRVYPDSVILIDRVA
                     WFDRAGLHLRDRLCPYDAIAETRIIDSPTRVTIDFLTVTGDAERLGRLRRGNLMTFSE
                     PITVGNQQFVRGLVLSITGDIEVLGEVNKDVISLFGDVYVGPGATARGDIAGISGAIT
                     VADKGSVYGELYSGGEYFERRRPRFYREENFDFLGAFVYNRVDGATPYLGLKFHDTDS
                     LLPSAWFRAGYALESERWRTELGIEQPLWRRVPLAAGAAYYRRLASEDDRLLGDEENT
                     AFALLATEDYRDYWEAEGATAWLSVKPAPQWRFETRYRYEETTWRRSYRHLWSLFGGS
                     KLFRENFSQVSEAERLAGAALIDTTALGCLAFVLDWDTHNREEPFAFSGWHATAELEW
                     SDRDFSSDFDYRRYTLTLRRYQKVHSHGMLLLRAMHGNSDGFLPMHKRFYLGGLGTLH
                     GYDFKEYAGTRFWMGNAEYRFEFPRSDIGASVFWDIGQIADGTKLDSAVDIKQSIGAA
                     LTVGADFRVSLARRLDRSFDNDPVVNVRLDFVF"
BASE COUNT          498 a          912 c          874 g          450 t
ORIGIN      
        1 gtaggcggac cggacctgga gctggttggc gtcgtccagc agcatcacca gcccccgctc
       61 cgcctgcatc agctcgatcg ctttgcgcat gacgatctgc agcacgtcgt cggtcaccag
      121 cgacgagttg atggccaggg aggcctccag gagcgccttg aggtcctcca cgctcttgga
      181 cgtgtgcgtt tcttcggacg tcaggcgcgg attggcctcg gccacctcgt ccagcgtggc
      241 ctccaggtcc gccaggacat cgagcggttc cacgtgctca gcgcccgctc cgacctggcc
      301 tccgaccccg gtcatgcgaa tcttggctgt cttctctatc tgggcctggt tcatgcgcgt
      361 acgttctcca gggtataacc tttttccact atatcggaaa atccgtcaag ggattaaccc
      421 gtgggcggac gcccggaacg cgcagacggc gctcagcggc gcgggaggac ttcgctgagc
      481 gaaaaggacc gcgaggtagt gctccactcc tcgcggccgt aattcgtcag cctcaggaac
      541 tcgcggaaca gggttttcac cggcgagacg ctcccggtgt cgtcatcgac agccaagttg
      601 atgccggtca aagcaatacg ggatatatct aatgtgatac gataatctcg gtcggcgggc
      661 agcccggcca gcgccgctac cctgaccacc accgagtccg ccagaaagcc ctccgcctcc
      721 tcccgcgaca gcaaaagccg ctccttcgcc gactggccgg gcccggaggt ctgcaccctg
      781 aaagcttctg tcaggagccg gtaggagagc cggatcgtgc aggagttctt agtcaccggg
      841 acggccccga agagtttgcg gggccgttgg agttcgatcg caatctcgca ggcgtagtcg
      901 atgccatcct gcagcgaccg gtattcgggg tggccgaaga agggagtcag attcagccag
      961 acagcgagcg agtcatcggc gcggacgatg tcgtactcga cgccgccggg gggatcggcc
     1021 ccgcgggccg gggcggcggc cgccaggatc agcgccagga gggccgccgg cgaccgcctc
     1081 cgtgcctgtg gccggaaaaa ccgcatctaa aatacaaagt ccagccgcac gttcactacc
     1141 gggtcgttgt cgaagctgcg gtcgagccgc cgcgccaggc tgacccggaa atcggccccc
     1201 acggtgagag ccgccccgat tgactgcttg atgtcgaccg ctgaatcgag cttcgtcccg
     1261 tcggcgatct ggccgatgtc ccagaacacc gacgcgccga tatcggagcg ggggaattcg
     1321 aaacggtact ccgcgttccc catccagaat cgcgtcccgg cgtactcctt gaagtcatag
     1381 ccgtggagcg ttccgagccc gccgagatag aaccgtttgt gcatcggcag gaaaccgtcg
     1441 gaattgccgt gcatagcccg cagcagcaac atcccgtggc tgtgcacttt ctggtagcgg
     1501 cggagcgtga gcgtgtaccg gcggtagtcg aaatcggaac tgaaatcgcg gtccgaccat
     1561 tccagttcgg ccgtggcatg ccagcccgag aaagcgaacg gctcttcccg gttgtgcgtg
     1621 tcccagtcga gcacgaacgc caggcagccg agcgcggtcg tgtcgatgag ggccgcgccg
     1681 gcgagacgct cggcctcgga cacctggctg aaattctcgc ggaacagctt gctgccgccg
     1741 aagagcgacc agaggtggcg gtaggagcgc cgccaggttg tctcctcgta gcggtagcgc
     1801 gtctcgaacc gccactgcgg cgccggcttg accgaaagcc acgccgtggc cccctcggct
     1861 tcccagtagt ccctgtaatc ctccgttgcc aacagcgcga acgcggtatt ctcctcgtcc
     1921 ccgagcagcc ggtcatcctc ggaggcgagg cggcgatagt aggccgcgcc cgccgcgagc
     1981 ggcaccctgc gccagagggg ctgctcgatg ccgagttcgg tgcgccagcg ttcggactcc
     2041 aacgcatacc ccgcccggaa ccaggccgag ggcagcagcg agtcggtgtc gtgaaacttc
     2101 agaccgagat acggcgtggc gccgtcgacc cggttgtaca cgaaggcgcc gagaaagtcg
     2161 aaattctcct cccggtagaa gcgggggcgc cggcgttcga aatactcgcc ccccgagtaa
     2221 agctccccgt agaccgatcc cttgtcggcc accgtgatcg cgccgctgat gccggcgata
     2281 tcgccgcggg ccgtggcccc cggaccgacg tacacgtcgc cgaacagcga gatcacatcc
     2341 ttgttcacct cgccgagaac ctcgatgtcc cccgtgatcg acagcacgag cccccggaca
     2401 aactgctggt tgccgaccgt gatcggctcg ctgaacgtca tgaggttccc gcggcgcagc
     2461 cgcccgagac gctccgcatc gccggtgacc gtgagaaaat cgatcgtcac ccgcgttgga
     2521 ctgtcgatga tccgcgtttc cgcaatggcg tcgtacgggc agaggcgatc gcggaggtgc
     2581 agaccggccc ggtcaaacca cgccactcgg tcaatgagaa tgaccgagtc gggatagacg
     2641 cgcacggccg cccgggggac cgatgcctcg gccggcaggc tgtcggccgc cgccgccacc
     2701 gagatttcat ccagcgtgaa agcgacgcgg aaaa
//
LOCUS       DAOOKS010000228         2731 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_45598_length_2731_cov_3.165172, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000228 DAOOKS010000000
VERSION     DAOOKS010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45598_length_2731_cov_3.165172"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="PK112_04555"
     CDS             <1..246
                     /locus_tag="PK112_04555"
                     /inference="COORDINATES: protein motif:HMM:NF024924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPC11353.1"
                     /translation="FCRQCRRCEPSCPHRADIPALMRTHMYAAQYANFEEARATLGEI
                     APGRGLHACGACERCAARCAHAVDIPGRIERLKTIYA"
     gene            complement(259..1146)
                     /locus_tag="PK112_04560"
     CDS             complement(259..1146)
                     /locus_tag="PK112_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11354.1"
                     /translation="MKHSFAGAAAAAALLALSVAGCGGKESAGTAGGPAQFLRTRLGA
                     AEPTGEVRTFTGETLWEYIDGDAEMYRQYRFVEIATADYRFPEVEFIVDVYRFADDAG
                     AYGLYAAVRPDEPDLLAIGTQGFCTESTVTAVKGRYLARLIAFDESSQTGAMLAVAAD
                     SLMRRLPGSTDRPAAFARFPDSARVPCGDRYYPASYLGQAFLTDVYEQAYALGPDTVR
                     LFFAADSSGGKFLRWQEAAGAEKVQTDLPFDGGAALMIRSYADTAAAGLRQFTLAGMR
                     AYAPRHRDFFARWLAALGG"
     gene            complement(1196..1753)
                     /locus_tag="PK112_04565"
     CDS             complement(1196..1753)
                     /locus_tag="PK112_04565"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HPC11355.1"
                     /translation="MDQHRADSADLHRMRRADRARDEAWISDLVARAPFCHIATVAGD
                     QPFITPMSFAYDPDRQALYFHTAGAGRLRTNTERGSRVCVSFAEMGRLLPAKTARGFS
                     VEYRSVIAFGRISLVDDPAAAARHMQMLIDRYFPRLRPGVDYRPIQPEEIEEISVYRI
                     DIDAWSGKEKSAPDEFPGAFSWESR"
     gene            1913..2572
                     /gene="rpe"
                     /locus_tag="PK112_04570"
     CDS             1913..2572
                     /gene="rpe"
                     /locus_tag="PK112_04570"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018118841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HPC11356.1"
                     /translation="MIEIAPSVLAADFSRLGAEIADTAAAGADRLHLDIMDGHFVPNI
                     SYGPDIVRTVNRLTGLPLDVHLMLTNPEAFFEPFAAAGADAITFHIEVHPDPVAHLKR
                     LEEMKVARGLSLNPDRTAESVLPFLELCDQLLVMSVFPGFGGQRFIEETLRTVEAARR
                     YIDRHRLRTQIAIDGGIDGNNAQRAVDAGADLLIMGSAFYRSTDRAGLVRKVHGLKRA
                     G"
BASE COUNT          445 a          896 c          981 g          409 t
ORIGIN      
        1 ttctgccgcc agtgccggcg gtgcgagccg agctgtccgc accgggcgga catccccgcg
       61 ctcatgcgca cgcacatgta cgcggcgcag tatgcgaatt ttgaggaggc gcgggcgacg
      121 ctcggggaga tcgcgccggg gcgcggactg cacgcctgcg gggcgtgcga aaggtgcgcg
      181 gcgcgctgcg cccacgcggt cgatatcccg ggccgcatcg agcgcctgaa aacgatctac
      241 gcctgaggcg ggtccgggct agcccccgag cgcggccagc caccgggcga agaagtcgcg
      301 gtggcgcggg gcgtaggcgc gcattcccgc cagcgtgaac tgccgcaggc cggcggccgc
      361 cgtgtccgca tacgaccgaa tcatcaaggc cgcaccgccg tcgaacggaa ggtcggtctg
      421 gaccttctcg gcgcccgccg cctcctgcca ccggaggaac ttcccgccgc tggaatcggc
      481 ggcaaaaaag aggcggaccg tgtcgggtcc gagcgcgtac gcctgctcgt acacatcggt
      541 gagaaacgcc tgcccgaggt acgaggcggg gtaatagcgg tccccgcagg gcacgcgggc
      601 ggaatcggga aagcgcgcga acgccgccgg gcggtcggtg ctccccggca gccttcgcat
      661 gagcgaatcc gcggccacgg cgagcatggc gccggtctgc gaagactcgt cgaatgcgat
      721 cagccgggcg aggtaccggc ccttgacggc ggtgacggtc gactcagtgc aaaatccctg
      781 ggtcccgatc gccagcaggt ccggctcgtc cggtcgcacc gcggcgtaga ggccgtaggc
      841 gccggcatcg tcggcgaacc ggtagacatc gacaatgaac tcaacctcgg ggaagcgata
      901 gtcggcggtg gcgatctcga caaagcggta ctggcggtac atctcggcgt cgccgtcgat
      961 gtattcccag agcgtttcgc cggtgaaggt gcgtacctcg ccggtcggtt ccgccgcccc
     1021 caggcgggtc cgaaggaact gcgccggtcc cccggccgtc ccggctgact ccttcccgcc
     1081 gcagccggcg acagagaggg cgagcagggc ggcggccgcc gcggcgcccg cgaacgaatg
     1141 cttcatagca accttcctcg atttcgatgc cggcgcatct tcagcgccgg ccggttcagc
     1201 ggctctccca ggaaaacgcg ccggggaatt cgtccggggc gctcttctcc ttgcccgacc
     1261 aggcgtcgat gtcgatccgg tagacggata tttcctcgat ttcctccggc tggatcggcc
     1321 ggtagtcgac gcccgggcgg aggcggggaa agtagcggtc gatgagcatc tgcatgtgtc
     1381 gagcggcggc ggcggggtcg tccaccagcg agattcgtcc gaaggcgatg acgctgcggt
     1441 attcgacgga gaagccgcgg gccgttttgg ccggcagcag ccgccccatc tcggcgaacg
     1501 acacgcacac gcgcgagccc cgctcggtgt tggtgcggag gcggccggcc ccggcggtgt
     1561 ggaaatagag ggcctgccgg tcggggtcat aggcgaagct catcggcgtg atgaacggct
     1621 ggtcacccgc gaccgtggcg atgtggcaga agggcgcgcg ggcgaccagg tcggaaatcc
     1681 aggcctcgtc gcgggcccgg tcggcgcggc gcatccggtg gagatcggcg ctgtcggcgc
     1741 gatgttgatc catgcgatcc atccttgtcc gagtgagagg ccgccggcgg aggcgacccc
     1801 gccgcaacat aggacggggg gggccggggg tcaagcaaaa ccgccccggc cgaggcgcgg
     1861 ggcgcgcatg tgaagacttg actccccgcc cgcgcccgtc ctacattgcg ccatgatcga
     1921 aatcgcaccg tcggttctcg ccgccgactt cagccgcctg ggcgcggaga tcgccgacac
     1981 agccgccgcc ggggccgacc gcctgcacct ggacatcatg gacggccact tcgtgccgaa
     2041 catctcctac ggaccggata tcgtccgaac cgtcaaccgt ctgaccggcc tgccgctcga
     2101 cgtccacctg atgctgacca atcccgaggc cttctttgag ccgttcgccg ccgcgggcgc
     2161 cgatgccatc acgttccaca tcgaggtcca ccccgacccg gtcgcccatc tgaaacgcct
     2221 cgaggaaatg aaggtggcgc gcgggttgtc gctcaatccc gacaggacgg ccgagtccgt
     2281 gctgccgttt ctggaactgt gcgaccaact tctggtgatg tcggtgtttc cgggattcgg
     2341 cggccagcgg ttcatcgagg agaccctgcg gacagtcgag gccgcccgac ggtatatcga
     2401 tcgccaccgc ctgcgcacgc agattgcgat tgacgggggg atcgacggca acaacgcgca
     2461 gcgggcggta gacgccggcg ccgacctgct gatcatggga tcggcctttt accggagcac
     2521 cgaccgggcg gggttggtgc gaaaggtcca tgggctgaaa cgggcaggat gaagcggggg
     2581 gcgtacttgc cgccggtatt cctgaggact tgtagcacat tgtgcaacag cagcatggct
     2641 cgcttgcggc ggggtggcgg cggccggata gcttagggga gccacgaaaa gggcggagtt
     2701 ccggttctga ttcgtgcggg aggagcggcc g
//
LOCUS       DAOOKS010000229         2728 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_45664_length_2728_cov_3.177329, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000229 DAOOKS010000000
VERSION     DAOOKS010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45664_length_2728_cov_3.177329"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="PK112_04575"
     CDS             complement(<1..674)
                     /locus_tag="PK112_04575"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HPC11357.1"
                     /translation="MHQGGHFGKFGAHVGFGPGLIEECEKTEGRFRSAVDISDGVLFA
                     IVSVREILEDNDARDRVGLFVKKNLMLIVSLRDDDESIPVTPDETVRRLNLKTITLER
                     LICAFFERLLSRDGSDLERFDHRVAEMEEQIENNTTGKKFNSEILSQRRKLLVIRNYY
                     EQLLDLFDAMLENESDLFSAKQLYHFRTARDKIARLSANAQLLRDSLVQVREAYMAAL
                     DYNANR"
     gene            636..1307
                     /gene="rlmD"
                     /locus_tag="PK112_04580"
     CDS             636..1307
                     /gene="rlmD"
                     /locus_tag="PK112_04580"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HPC11358.1"
                     /translation="MRAELPEVTTLVHNQNGRISNIAVGESEQVLFGLGYIEEQLFDC
                     RFRIRANSFFQTNSIQAETLYRTCFEMLRPGPSERVFDLYCGTGSIAILLAGYVQHVV
                     GVELVKDAVDAAVENAACNNVGNVEFHHADVKDYLALIEGSSVRFDTVIVDPPRTGLH
                     PKALRRLLRLDPPRILYVSCNPATFARDARLIVDAGYDLPLVRPVDMFPHTMHIELAG
                     VFNRR"
     gene            1394..>2728
                     /locus_tag="PK112_04585"
     CDS             1394..>2728
                     /locus_tag="PK112_04585"
                     /inference="COORDINATES: protein motif:HMM:NF017745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HPC11359.1"
                     /translation="MIGTRMLWALAAALFLCGPAPAAAAAEPGLDNLCDQILAALQAF
                     YPVRSTEMGIHDYDHRFTDYSSSSVRDMVKRLAAFDKKLAKYRAADLPADQAIRCRLI
                     QSNVDAALLDLKQISWHTRLPQLYVDEAVNGLYLLLGSRSLAPEQAAVSVLARMKAVP
                     ALLETARKNIRRPPQVWIDEAVSALVSAEVFYGDAGAEMARQFPARAEEFRTAAAAAQ
                     EVLRGFSDWLRRAEPGDPGAFAIGADNFNYKLAHEHFLPYTADSLLALGEALLVDADS
                     AYDEYRRFVEEEAQNGSERVFIPRCFSRADILDYYAWEVAQVRAFCADNEIVTVPQNI
                     APVAVVETPPYLQSMITGIAYQPAGPFDTAQQALFFVRPVPDPLDSAQLSVRARYIHR
                     RGFRGSVVHEAYPGHHLQTQVAGMHDDPVRKWQSNSLAVEGWALYCEQMMYEA"
BASE COUNT          477 a          969 c          761 g          521 t
ORIGIN      
        1 atgcggttcg cgttgtaatc cagcgcagcc atgtatgctt cccgcacctg caccagactg
       61 tcccgcagca gctgtgcgtt tgcggacagc cgcgcaatct tatcccgtgc ggtgcgaaaa
      121 tgatacagtt gtttggctga gaacaagtcc gattcgtttt ccagcattgc gtcaaagaga
      181 tccagcaatt gctcataata gtttcgaatc acaagcagtt tgcgtctttg cgacaagatt
      241 tcactgttga atttcttacc ggttgtgttg ttctcaatct gttcttccat ctcagcgaca
      301 cgatggtcaa atcgctccag atcgcttcca tcgcggctca gcagccgctc aaaaaacgcg
      361 caaatcaggc gttccagcgt aatcgtcttt aggtttagac gacgcaccgt ctcatccggc
      421 gtgaccggaa tgctctcgtc gtcgtcccgc agggatacga tcagcatcag gttctttttc
      481 acaaacagtc cgacacgatc gcgcgcatcg ttatcctcta aaatctcgcg tacggatacg
      541 atcgcaaata aaacgccgtc tgaaatgtct acagcagaac gaaaccgtcc ctccgtcttt
      601 tcgcattctt caatcagccc cgggccgaaa ccgacatgcg cgccgaactt cccgaagtga
      661 ccaccctggt gcacaaccag aacggccgga tatcgaacat cgccgtaggc gagagcgagc
      721 aggtgctctt cgggctcggg tacatcgagg agcagctctt cgactgccgc ttccgcatcc
      781 gcgccaactc gttctttcag accaactcca tccaggccga gaccctctac cgcacctgct
      841 tcgagatgct ccggcccggc ccctccgagc gcgtgttcga cctctactgc ggcaccggct
      901 ccatcgccat cctgctggcc ggctacgtcc agcacgtcgt cggggtcgaa ctggtgaagg
      961 acgcggtgga tgcggccgtc gagaacgccg cctgcaacaa cgtcggcaac gtcgaattcc
     1021 accacgccga cgtcaaggac tacctcgcgc tgatcgaggg gagcagcgtt cgcttcgaca
     1081 ccgtcattgt cgatcctccc cggaccggcc tgcaccccaa ggctctgcgg cgcctgctcc
     1141 gcctggaccc tccccggatc ctctacgtct cgtgcaaccc ggcgactttc gcgcgcgatg
     1201 cccggctgat cgtcgacgcc ggctacgacc tgccgctggt ccgcccggtc gacatgttcc
     1261 cccacacgat gcacattgaa ctcgccggcg tcttcaaccg gcgctgagag ccctccggcg
     1321 ccgggccccg acttttcttg ccccggagcg gattcccccg tacattggcg gatggaaaag
     1381 gaggacgcat tcgatgattg gcacccgcat gctctgggcg ctcgccgccg ccctgttcct
     1441 gtgcggtccg gcgccggccg cggccgccgc tgaacccggc ctcgacaacc tctgcgatca
     1501 gatcctggcc gccttgcagg ccttctaccc tgtccgctcc accgaaatgg gcattcacga
     1561 ctacgaccac cgcttcacgg actactcctc ctcgtccgtg cgcgacatgg tcaagaggct
     1621 tgccgcgttc gacaagaaac tcgccaagta ccgggcggcc gacctcccgg ccgaccaggc
     1681 catccgctgc cggctcatcc agtcgaacgt cgatgccgcg ctgctcgatt tgaaacagat
     1741 atcgtggcac acccggctcc cgcagttgta cgtcgatgaa gccgtcaacg ggctgtacct
     1801 cctgctcggt tcgcggagcc tcgcccctga gcaggcggct gtgtcggtcc tggcgcgcat
     1861 gaaggcggtg ccggcgctgc tggagacagc gcgcaaaaac atccgccgtc ccccgcaggt
     1921 ctggattgac gaggcggtga gcgccctggt ctccgcggag gtcttttacg gcgacgccgg
     1981 ggcggagatg gcgcggcagt ttccggcgcg ggccgaggaa ttccgcaccg ccgccgccgc
     2041 cgcgcaggag gttctgcgcg gcttttccga ttggctgcgg cgggccgagc cgggcgaccc
     2101 cggcgccttc gccatcggcg ccgacaattt caactacaag ctcgcccatg agcacttcct
     2161 cccctacacc gccgactccc tcctcgccct gggcgaggcg ctgctcgtcg atgccgactc
     2221 cgcgtacgac gagtaccggc ggtttgtcga agaggaggcc cagaacggca gcgagcgcgt
     2281 cttcatcccg cgctgtttct cgcgcgccga catcctcgac tactatgcgt gggaggtggc
     2341 gcaggtgcgc gccttctgcg ccgacaacga aatcgtcacc gtcccccaga acatcgcccc
     2401 cgtcgcggtc gtcgaaaccc cgccgtacct gcagtccatg atcaccggca tcgcctacca
     2461 gccggccggt ccgttcgaca ccgcccagca ggctctcttt ttcgtccgcc ccgttcccga
     2521 cccgctcgat tcagcgcagt tgtcggtgcg cgcccgctat atccaccggc gcggtttccg
     2581 cggctcggtc gtccacgagg cctaccccgg tcaccatctg caaacccagg tcgccggcat
     2641 gcacgacgac ccggtgcgca aatggcagag caacagcctc gcggtcgagg gctgggccct
     2701 ctactgcgaa cagatgatgt acgaggcc
//
LOCUS       DAOOKS010000230         2716 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_45932_length_2716_cov_3.237505, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000230 DAOOKS010000000
VERSION     DAOOKS010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45932_length_2716_cov_3.237505"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..852)
                     /locus_tag="PK112_04590"
     CDS             complement(<1..852)
                     /locus_tag="PK112_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11360.1"
                     /translation="MSATLLLPLSSHALQEPSQFIRSVAAAVAQGDSLPDHYSRTTTH
                     IVNQLALGVSNRGAIAQSGRDAFTGDTILGHCEFPRGSRRDYLEQIDLWLGVQGSRDP
                     VVSNGYYYLAEEAIAGEWLPSSPPGEFVRRSITDRFGGPDAEAVSEEDLLCTYDDSLG
                     LWKLGDHLAPNPLSLGAFVRQASYAWSMTYAEDFAIVRLDIRNEGTMLWRETYAGLHI
                     IPSVGLRDPCEDCGPKWTVCGIRTVAPAAFGCDHLDTLNTAWHATASGDPYEGEWLEN
                     PLYDSFRK"
     gene            complement(1063..>2716)
                     /locus_tag="PK112_04595"
     CDS             complement(1063..>2716)
                     /locus_tag="PK112_04595"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPC11361.1"
                     /translation="RQGTLPKIALAVLLVIPLFLLWVFYVSPRRQRFLTRLRRPIILL
                     AGLLIVILPVTVRNLLVTGDFILISSQGGVNLYLGNNPLANGLSMQMGEVELTEGLTW
                     DRFAPAAHEAAERLAGRRLSESESSDFWTRRSLQFIKDNPAAFAGLVWRKTVYLVSGF
                     ENSDNSDIYYERGKSFLYSLLLWDGPIDFPFGLLLPLTLAGLYLRRRETDRLAPLYLF
                     LIGYIPTIVLFLVTARHRLPLVPFMIILAAAGLVKAVELTRRRRLKELAVAGVIFLVP
                     LVLCNRLWYDEGGRNEFQVHFNEGIKYEHLGDYAAAEREYLAADRSFGGSAALLTNLG
                     RVQHQLGKLDEADRAYRRALGLDPTFYRALNNLGLLVGERGNLDSSLVLFVAARQHYD
                     SMLARTNELGLIYMNIGKGLEQFGRPDVAVAYFDSAVHAAPQSPEVSYQAAAFFARQE
                     RYELSDSLFLRGQALTEPSAADFFNWGLSFMERRRFADGIYMLDKALRRDSTLYQAHY
                     LIAVAYREGGMPPDSVNAHLNAALRSNPNYEPALRFREQIGR"
BASE COUNT          546 a          793 c          943 g          434 t
ORIGIN      
        1 cttcctgaaa gaatcataaa gcgggttttc cagccactcg ccctcgtacg gatcgcccga
       61 ggcggtggca tgccaggcgg tattgagagt atcaaggtga tcgcagccga aagccgccgg
      121 cgccacagtg cgaatgccgc agactgtcca tttgggtcca caatcctcgc acgggtcgcg
      181 aagtccaact gagggtatga tgtgaaggcc ggcgtaggtc tcacgccata gcatagtgcc
      241 ctcattgcga atgtctaatc gaacgatagc gaagtcctcg gcataagtca tcgaccatgc
      301 atacgatgcc tgcctgacga aagcgccgag gctgagcgga ttcggggcga ggtggtcccc
      361 gagtttccac aggccgaggc tgtcatcata cgtgcagagg aggtcttcct cagagacagc
      421 ttccgcatcg ggtcctccga aacggtcggt gatcgaccga cggacaaatt cgccaggcgg
      481 cgaagagggg agccattctc cggcaatcgc ttcctcagca aggtagtagt agccgtttga
      541 gacaaccggg tcgcggctgc cttggacacc gagccataga tcgatctgct ccaggtagtc
      601 gcgcctgctc ccgcgcggga actcgcaatg accgaggatg gtatcgccgg tgaaggcgtc
      661 gcgccccgac tgtgcgatgg cgccgcgatt ggagacgccg agagcgagct ggttgacgat
      721 gtgggtggtc gtacgggagt aatggtcggg cagagagtct ccctgcgcaa ccgccgctgc
      781 cacggagcga atgaactgtg acggctcctg cagcgcatgg gaagagaggg ggagaagcag
      841 ggtcgcggac atcactgcga tcttggtccc cagtttccgc gaattggcca ttaggccccg
      901 gtggttcccg ctcatctccg accccactgc ttatgcaaat cggcagagta ctctcttgaa
      961 ggataaagtg tgcgcggcga cacgtcaagc gcagacgaca gggcgccgac agtcttcgga
     1021 tgtcgcgttc cccggacccc gacctcccgc cttgccgggc gcttagcgcc cgatctgctc
     1081 gcggaagcgg agagccggtt cgtagttcgg gttggaccgg agagcggcgt tcaggtgggc
     1141 gttcacggaa tccgggggca tgccgccctc gcggtaggcg accgcgatca ggtaatgggc
     1201 ctgatagagc gtggagtcgc gccggagcgc tttgtcgagc atgtagatgc cgtcggcgaa
     1261 ccgcctccgc tccatgaacg agagccccca gttgaaaaag tcggcggcgg agggttcggt
     1321 cagcgcctgg ccgcgcagga aaagcgagtc cgacaactcg tagcgctcct ggcgggcaaa
     1381 aaacgcagcc gcctggtagg agacctccgg cgactgagga gcagcgtgaa cggcggaatc
     1441 gaaataggcg accgccacgt cggggcggcc gaactgctcg agccctttgc cgatattcat
     1501 gtagatcagc ccgagttcgt tggtacgggc gagcatgctg tcgtaatgct gccgggccgc
     1561 gacaaacagg acgagcgacg aatccagatt accgcgctcc cccaccagca ggccgagatt
     1621 gttcagggcg cggtaaaacg tgggatcgag acccagggcg cgccggtaag cccggtcggc
     1681 ctcgtcgagc ttgcccaact ggtgctgtac gcggccgagg ttggtcagga gagccgccga
     1741 tccgccgaac gaccggtcgg cagccaggta ttcccgctcg gcggccgcgt agtcgcccag
     1801 gtgctcgtac ttgattccct cgttgaaatg cacctggaat tcgttgcggc ccccctcgtc
     1861 gtaccacagg cggttgcata acacgagcgg gaccaggaag atgacgccgg cgaccgccag
     1921 ctccttcagg cgccgccggc gggtgagctc gacggctttc accagaccgg ccgccgcgag
     1981 aatgatcatg aacggaacca gcggcaggcg gtggcgggcg gtcacgagaa agagcacgat
     2041 cgtcgggatg tagccgatga ggaagaggta cagcggggcc agcctgtcgg tctcccggcg
     2101 gcgcaggtag aggccggcga gagtgagcgg caacaggagg ccgaagggga aatcgatcgg
     2161 accgtcccac agcaagagcg aatagaggaa ggacttgccg cgctcgtagt agatgtcgga
     2221 gttgtcggaa ttctcaaaac cgctcacgag gtacaccgtc ttgcgccaga ccagcccggc
     2281 gaacgccgcg gggttgtcct tgatgaattg cagcgagcgg cgcgtccaga agtcggagga
     2341 ttcggattcg gacaggcggc ggccggccag ccgctcggcg gcctcgtggg cagcgggcgc
     2401 gaagcggtcc caggtgagcc cctcggtcag ctccacctcc cccatctgca tcgacagccc
     2461 gttggccagc gggttgttgc ccaggtagag gttcacgccc ccctgcgagg agatgaggat
     2521 gaagtcgccg gtgacaagga ggttgcggac ggtcaccggg aggatgacga taagcaggcc
     2581 ggccaggagg atgatcgggc ggcgaaggcg ggtcaggaat ctctggcggc ggggggaaac
     2641 gtagaacacc cagagcagga acagcgggat gacgagaagc acggcgaggg cgatcttggg
     2701 cagggtgccc tggcga
//
LOCUS       DAOOKS010000231         2704 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_46221_length_2704_cov_3.413364, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000231 DAOOKS010000000
VERSION     DAOOKS010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46221_length_2704_cov_3.413364"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="PK112_04600"
     CDS             <1..557
                     /locus_tag="PK112_04600"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HPC11362.1"
                     /translation="RLCAMTDKPLPRKRSYLRTPAPTRPIPVDFAVLHEQGAGAPQMF
                     AHLTRELARRYAINRGVLIMRRGAAGPFAAVSTWVNGSLRDGLAVNLPPQSSLFEKVA
                     EHGRVYTENFCASFSGNFFERKLLLGDESRSFVVQPLTSDGEVVGLVAYSSDEPTAFA
                     MFEEGAVEDVARAFAAAIRKQRLA"
     gene            complement(577..1284)
                     /gene="mltG"
                     /locus_tag="PK112_04605"
     CDS             complement(577..1284)
                     /gene="mltG"
                     /locus_tag="PK112_04605"
                     /inference="COORDINATES: protein motif:HMM:NF014656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HPC11363.1"
                     /translation="MLDRLARGDLLRIRVTIPEGTTLWRTAGLLARSLELDSAAIVGL
                     GSDSAFLAELGKPCLEGYLFPETYFFPWGIDEREAVRTAVAQFDRQTEGLWAGERTLG
                     LSPHEIVILASIIESETPLAGERGLVASVYANRLRQKMRLDADPTVIYGLGGLDRPLS
                     RRDLRKDTPYNTYLHAGLPPTPINSPGLASIKAALAPDTSDYLFFVADNAGGHFFSKT
                     NAEHERARRRIRQERGR"
     gene            complement(1239..1562)
                     /locus_tag="PK112_04610"
     CDS             complement(1239..1562)
                     /locus_tag="PK112_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11364.1"
                     /translation="MGRRIVTAAAVLLAAAIIGLAVGQHYRTVDLGDRVATVIIAPGD
                     SFAQVAARLDAQGVVPSPRLLRLAARLTGVDRRLIPAGMISRGNSRRPACSTAWPGGI
                     CSASG"
     gene            complement(1633..2070)
                     /gene="ruvX"
                     /locus_tag="PK112_04615"
     CDS             complement(1633..2070)
                     /gene="ruvX"
                     /locus_tag="PK112_04615"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HPC11365.1"
                     /translation="MAAGGDTRTLLGIDYGARRIGVAKSDPTGLIATALTTLVVKSAA
                     DALAQVAALIREHQPAGIVIGSPVGLSGESNRKCLEIEAFANALRTVFDGPIHLWDEG
                     YSSVEAADIIHAHGKRVGKDKKRIDRLAAVIILQRFLDEQPER"
     gene            complement(2075..>2704)
                     /locus_tag="PK112_04620"
     CDS             complement(2075..>2704)
                     /locus_tag="PK112_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HPC11366.1"
                     /translation="YDYVRRGGFLFSMCSAPETIDIALAAEGVDISPSEFDGDPVDPN
                     CQARLDYSKTFAFENFTVELNPLEYRHSDIDTYPDRQIRFLSPDEDYFFLFDFAAKLD
                     PVPTMLVQDHTATVAGFMGQTTGFRKSLLKDFVTILGQPDNYDEVRYIHGNLGRGTFT
                     FLSGHDPEDYRHMVHDPATDLALYKNSPGYRLILNNVLFPAAKRKERKT"
BASE COUNT          439 a          959 c          854 g          452 t
ORIGIN      
        1 cgaggctgtg cgcgatgacg gataagccgc tgccgcgcaa gcggtcatac ctgcggacgc
       61 ccgccccgac ccggccgatt ccggtcgatt tcgccgtcct gcacgaacag ggggcggggg
      121 cgccccagat gttcgcccac ctgacgcgcg agctcgcccg tcgctacgcc atcaaccggg
      181 gcgtgctgat catgcgccgc ggcgccgccg gccccttcgc cgccgtctcc acctgggtga
      241 atggatcgct ccgcgacggt ctggcggtca accttccccc gcagtcctcg ctgtttgaaa
      301 aggtcgccga acacggccgc gtctacacgg agaacttctg cgcctccttc agcggcaact
      361 tcttcgagcg caagctcctg cttggcgatg agtcccgctc ctttgtcgtc cagccgctca
      421 ccagcgacgg cgaggtggtc ggcctggtgg cctacagctc ggacgaaccg accgcctttg
      481 cgatgttcga ggaaggggcg gtcgaggatg tggcccgggc cttcgcggcc gcgatacgaa
      541 agcagcgcct cgcctgaacc gcctccggcc gagcgcctac cggccgcgct cctgccgtat
      601 ccgccggcgc gcccgctcat gctccgcatt ggttttcgag aagaagtgcc cccccgcgtt
      661 gtcggccacg aagaacaggt agtcgctcgt gtcgggagcg agcgcagcct tgatcgaggc
      721 cagtcccggc gagttgatcg gcgtcggcgg aagccccgcg tgaaggtagg tgttgtaggg
      781 cgtgtccttg cgcaggtcgc gccgggagag cggccgatcg agtcccccca gaccgtagat
      841 cactgtcggg tcggcatcca gccgcatctt ctgccgcagc cggttagcat agaccgaggc
      901 caccagcccg cgctcgccgg ccagcggcgt ttccgattcg atgatcgagg ccagaatgac
      961 aatctcgtgc ggcgagagcc ccagcgtcct ctcccccgcc cagagtccct cggtctgccg
     1021 gtcgaactgc gccacggccg tccgcaccgc ctcccgctcg tcgattcccc aggggaagaa
     1081 gtacgtctcg gggaagaggt atccctccag gcagggtttg ccgagttcgg cgagaaacgc
     1141 gctgtcgctg cccaggccca cgatcgccgc cgaatcaagt tccaggctgc gggcgagcag
     1201 accggccgtc cgccagagcg tcgtgccttc gggaatcgtt acccggatgc ggagcaaatc
     1261 cccccgggcc aggcggtcga gcacgccggc cgccgactgt ttccccgtga aatcataccg
     1321 gccgggatca gcctgcggtc cacaccggtg agtctcgccg cgagccgcag gaggcgcggc
     1381 gacggcacca ctccctgggc gtccaaccgg gcggccacct gggcgaacga gtcgcccggg
     1441 gcgatgatca cggtcgccac ccggtctccc aggtccaccg tgcgatagtg ctgtccgacc
     1501 gccagcccga tgatggccgc cgccaaaagc acggccgccg ccgtgacgat tcttctaccc
     1561 ataatttctg ccgcgaggac cggccgggcg accgccgcca cccgccggag ctgctccctc
     1621 tgtcacaccc ccctaccgtt ccggctgctc atcgagaaac cgctgcagaa tgatcaccgc
     1681 cgccagccga tcgatccgct tcttgtcctt gccgacccgc ttgccgtggg catggatgat
     1741 gtccgccgcc tcgaccgagg agtagccctc gtcccacagg tggatcggcc cgtcgaaaac
     1801 cgtgcggagc gcgttcgcga aggcctcgat ctcgaggcac ttccggttcg actcgcccga
     1861 cagcccgacc ggggatccga tgacgatgcc cgcgggctgg tgttcgcgga tcagcgcggc
     1921 cacctgggcc agcgcgtcgg ccgccgattt caccaccagc gtcgtcaacg ccgtggcgat
     1981 cagcccggtc ggatcgctct tggcgacccc gatccggcgg gcgccgtaat caatcccgag
     2041 aagagtgcgc gtgtcgcctc ccgccgccat accctcaggt cttccgttcc ttccgcttgg
     2101 ccgcggggaa cagcacgttg ttgagaatca gccggtaccc cggcgagttc ttgtagagcg
     2161 cgagatcggt cgccggatcg tgcaccatgt gccggtaatc ctccggatcg tgcccggaca
     2221 aaaacgtgaa tgtcccccgc ccgagattgc cgtggatgta ccggacctca tcgtaattgt
     2281 ccggctgacc caaaatggtc acgaaatcct tcagcagcga cttgcggaaa ccggtcgtct
     2341 gccccatgaa cccggccacc gtcgccgtgt gatcctgcac cagcattgtc ggcaccgggt
     2401 cgagcttggc cgcaaaatcg aacaggaaga agtagtcctc gtccggactg aggaatcgaa
     2461 tctggcggtc cggataggtg tcgatgtcgg agtggcggta ctccaggggg ttcagttcca
     2521 cggtgaaatt ctcgaacgcg aatgtcttgc tgtagtcgag ccgggcctgg cagttcgggt
     2581 cgaccgggtc gccgtcgaac tcgctgggcg agatatccac gccctcggcc gccagcgcga
     2641 tgtcgatcgt ctccggcgcc gagcacatgg agaacaagaa cccgccccgc cgcacgtaat
     2701 cgta
//
LOCUS       DAOOKS010000232         2702 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_46282_length_2702_cov_3.615036, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000232 DAOOKS010000000
VERSION     DAOOKS010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46282_length_2702_cov_3.615036"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="PK112_04625"
     CDS             <1..682
                     /locus_tag="PK112_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11367.1"
                     /translation="MTAGLKGGRFLYYIEVRDKIGGQRAVFLDGGRPFVMKYVGHVPT
                     WLLLTHIALMFATVYFVVIGALHGARLTGGGTDVFPMARAYGIAALCAFLGGYPFGIP
                     MNWFTFGTTWEGVPFGTDATDNKTQLLFVYLLLVVFAAFGSLTRRRVGRDAYPPRVLG
                     WLGVLGFAVMLGIYLIPHSIQFSPALTRIVCWSFIGLAAAAYFGGLAAGIVRRRAPRA
                     HKAERRAG"
     gene            679..1539
                     /gene="prmC"
                     /locus_tag="PK112_04630"
     CDS             679..1539
                     /gene="prmC"
                     /locus_tag="PK112_04630"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HPC11368.1"
                     /translation="MTNRLRPLLARYAAQLAAAGIEQAEAELEWIFEHVLGVDRLRLY
                     LEGDDLLTDAAAAQADAIVQRRLTRHPLQFILGEAWFYGRKFSVSEAVMAPTPETELL
                     VETAVRSVGSRGLAAPRVLDVGVGSGVIAVTLALELPACRVTAVDISAEALAVARQNA
                     AAHGVRDRVDLRVSDFFSAVAADERFDLIVSNPPYIAEPDYAGLAPEVRADPKIAMTA
                     GPEGLDAIRVLVQEAPAHLVPGGRLMFEIGHDQADKVAALTARDPRYRTFVLLRDLND
                     RDRVIILGGD"
     gene            1539..2219
                     /gene="nth"
                     /locus_tag="PK112_04635"
     CDS             1539..2219
                     /gene="nth"
                     /locus_tag="PK112_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HPC11369.1"
                     /translation="MPREGAAARRERAAEIVRILRRHYPGACTTLAYGSVHQLLVATI
                     LSQQNTDKGVNAVTPRLFRKYPTIEAFANADVDDLRADIASINYGPTKARAIIESALQ
                     ILEQFGGEVPRTIGELVRLRGVGRKTASVVLGAGYGIAEGVVVDTHVIRVSGRLGLTA
                     HSDAARIERDLMKILPQRDWIDWTHMVILHGRAVCTARRPACPACPLAARCPAAFKFP
                     HLERGGGR"
     gene            2303..>2702
                     /locus_tag="PK112_04640"
     CDS             2303..>2702
                     /locus_tag="PK112_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012508066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HPC11370.1"
                     /translation="MAYQQPEVTPEMVAAHGLTKEEHERIREILGREMTWTELGIFSV
                     MWSEHCSYKNSIALLKTLPREGEALLAKAGEENAGAVDIGDGLAVVFKIESHNHPSAV
                     EPYQGAATGVGGILRDIFTMGARPIAALDSL"
BASE COUNT          397 a          867 c          988 g          450 t
ORIGIN      
        1 gatgacggcc gggctcaagg ggggccggtt cctgtactac atcgaggtgc gcgacaagat
       61 cggcgggcag cgtgcggtgt ttctcgacgg cgggcggccg tttgtgatga aatatgtcgg
      121 ccatgtgccg acctggctct tgctcaccca cattgccctg atgtttgcga cggtgtattt
      181 cgtggtgatc ggcgctctcc acggggcgcg gctgaccggc gggggcacgg acgtgtttcc
      241 gatggcgcgc gcctacggca ttgccgccct gtgcgcgttt ctgggcggct acccgttcgg
      301 cattcccatg aactggttca ctttcgggac gacctgggag ggcgtgccgt tcggcaccga
      361 cgccaccgac aacaagacgc agctgctgtt cgtgtacctc ctgctggtcg tttttgccgc
      421 gttcggctcg ctcacccggc gccgggtggg ccgcgacgcg tacccgccgc gggtgctcgg
      481 ctggctgggc gtactcgggt tcgcagtcat gctggggatc tatctgatcc cgcattccat
      541 ccagttctcc cctgcgctga cgaggatcgt ctgctggtcg ttcatcggcc tggcggcggc
      601 cgcgtatttc ggcggattgg cggccggaat cgtgcggcgg cgggccccgc gggcgcacaa
      661 ggcggagcgg cgcgccggat gaccaaccgg ctccgaccgc tgctggcccg gtacgccgcc
      721 caactggcgg cggcgggaat cgaacaggcg gaggcggagc tcgagtggat tttcgaacac
      781 gtgctcggtg tcgaccggct ccggctctat ctcgaagggg acgaccttct caccgacgcg
      841 gcggcggcgc aggccgacgc cattgtccaa aggcgcctga cccgccaccc gctccagttc
      901 attctcggcg aagcctggtt ctacggacgg aaattctcgg tcagcgaggc ggtcatggcg
      961 ccgacgccgg agacggaatt gctggtcgag acggcggtgc ggtcggtggg gagccgaggt
     1021 ctcgccgcgc cgcgcgtgct cgacgtcggg gtcggctcgg gcgtgatcgc ggtgaccctt
     1081 gcgctcgaac tacccgcgtg ccgggtgacg gcggtggaca tatcggccga ggcgctcgcg
     1141 gtggcgcggc aaaacgcggc cgcccacggc gtccgcgacc gcgtcgacct gcgggtgtcg
     1201 gatttttttt cggcggtggc cgccgacgag cggtttgacc tgatcgtctc caacccgccg
     1261 tatatcgcgg agccggatta cgccgggctg gcgccggagg tgcgggcgga tccgaagatc
     1321 gcgatgaccg ccggccccga ggggcttgac gcgatccggg tcctcgtgca ggaggccccg
     1381 gcccacctcg tcccgggcgg acggctcatg ttcgaaatcg gccacgacca ggcggacaaa
     1441 gtcgctgcgc tcaccgcccg cgacccgcgc taccgcacgt tcgttctcct ccgcgacctc
     1501 aacgaccgcg accgcgtcat catcctgggg ggcgactgat gccgcgcgag ggcgcggccg
     1561 cccgccggga gcgggcggcg gagatcgtcc gcatcctcag gcggcactat cccggcgcgt
     1621 gcaccacgct ggcttacggc tcggtccacc agttgctcgt ggcgaccatt ctgtcgcagc
     1681 agaataccga caagggggtg aacgcggtga ccccccggct gtttcggaag tacccgacga
     1741 tcgaggcgtt cgcgaacgcc gatgtcgacg acctgcgggc ggacatcgcg tcaatcaact
     1801 acggcccgac caaggcgcgg gcgatcattg agtcggccct gcagattctc gagcagttcg
     1861 gcggggaggt tccccgcacg atcggtgagc tggtgaggct gcgcggcgtg gggcggaaga
     1921 ccgcctcggt ggtgcttggg gccgggtacg gcatcgccga gggggtggtg gtcgataccc
     1981 acgtgatccg ggtgagcggg cggctggggc tcacggcgca ctcggatgcg gcccggatcg
     2041 agcgcgacct gatgaaaatc ctgccgcagc gcgactggat cgactggacg cacatggtca
     2101 tcctgcacgg gcgggcggtc tgcacggcgc gccgccccgc ctgtcccgcc tgcccgctgg
     2161 cggcccgctg ccccgcggcg ttcaaattcc cccacctgga gaggggcggg ggacgttgac
     2221 cgggggacgc cgggaagata tattggcctg tcacaccggc ggcggcgccg gagaaacatc
     2281 ggtcgaaagg gacagaaccc agatggcgta tcagcagccg gaggtgactc cggagatggt
     2341 tgcggcccac gggctgacta aagaggaaca cgagcggatc cgggagatcc tcggtcggga
     2401 gatgacctgg acggagctgg ggatattctc ggtcatgtgg tcggagcact gctcgtacaa
     2461 gaactcgatc gcgctcttga agacactccc gcgggagggc gaggcgctct tggccaaggc
     2521 gggcgaagag aacgccggcg cggtggatat cggcgacggt ttggcggtcg tgttcaagat
     2581 cgagtcgcac aaccacccct cggcggtgga gccctaccag ggggcggcga cgggcgtggg
     2641 gggaattctg cgcgacattt tcaccatggg tgcgcggccg atcgccgcgc ttgactccct
     2701 cc
//
LOCUS       DAOOKS010000233         2675 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_46936_length_2675_cov_2.495038, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000233 DAOOKS010000000
VERSION     DAOOKS010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46936_length_2675_cov_2.495038"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..1366
                     /locus_tag="PK112_04645"
     CDS             113..1366
                     /locus_tag="PK112_04645"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPC11371.1"
                     /translation="MLMTFVEMRDCVLMALGSLRANKLRSGLTILGVLIGVASVIGLA
                     SLINGLNAAMEQEISGMGAAAIIEVERLPAMRDWDDLTEEERNRPELTVGEAEAIRDH
                     CPSVDGVAPTNHYWAPGGNVVKYQNRKVPNVRYFGTWPDYMRVRERSLQQGRFLGSMD
                     EARRAHVAVIGHDIANTLFEGRNPIGQEIRVNDERFEVIGVFEKAKSNFGNTYENNAV
                     AIPLSTFEKIHPWEEALGLSVRAVTLDKLEQAKEEVIGALRVYRKVPFGKENNFDLLT
                     QDAIREQVEKITDYIYIAMLVITSVGLMVGGIGVMNIMLVSVTERTREIGVRKAIGAK
                     RSNILLQFLTEATSLSGAGGTAGIIIGVLFGVAVNSAIGFPIAISVFWIVVGFLVAVS
                     VGMISGMYPAVKAAKLDPIEALRYE"
     gene            1471..>2675
                     /locus_tag="PK112_04650"
     CDS             1471..>2675
                     /locus_tag="PK112_04650"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HPC11372.1"
                     /translation="MVRTFLRLTVLGLLVWVVAAAAAFGQSPLLRRAITGEAKKLIVG
                     TGVFPSEEPGRVRLEVYYQVYNYALDFQQLGGLFRARYVTTIRVNDRKADTTAAVSRE
                     EREAVVDALAKTTSTADYRAAQVNFDLPPGDYEVLFLIENVGSSAILKRDFDLRLEPF
                     DSRSAELSDIELAQAAGPNESDSAAGAFAKGEFIVVPSLTGSFSGDDGGRLLFYLEIH
                     RGGDSLSEVTVVTALRHESKGMVYRDSLTTALPQRITRQLREISLDGFMPGDYRLEVS
                     LHGRRYKQLDQRQAEFRIPWTAASVLRYDWNAALDQLELIADGNEIKPLRKIDSLADR
                     VAALDRFWAERDPDPTTAVNKIKVVFYYRVDLANRLFSILNMEGWRTDRGRVLIRYGE
                     PDQIDDYPLV"
BASE COUNT          471 a          908 c          832 g          464 t
ORIGIN      
        1 tctcggcctg ggcatttcca cctcggttgg gctcatcttc ggtctgtacc cggcgatgaa
       61 ggccgcccgg ctcgacccca tcaaggcgct gagctatgag tgaggacggc ccatgctgat
      121 gacctttgtc gaaatgaggg actgcgtgct catggcgctc ggctctctgc gcgccaacaa
      181 actgcgctcc gggctgacca tcctcggggt gctgatcggc gtcgcctcgg tcatcggcct
      241 ggcctcgctc atcaacggcc tcaacgccgc catggaacag gagatctccg gcatgggcgc
      301 ggccgccatc atcgaggtcg aacgcctccc cgccatgcgc gactgggacg acctgaccga
      361 ggaggagcgc aaccggccgg agttgacggt cggagaggcg gaggcgatcc gcgaccactg
      421 cccgtcggtc gacggcgtgg cgccgaccaa tcactactgg gcccccggcg gcaacgtcgt
      481 caaataccag aaccggaaag tccccaacgt gcggtatttc ggcacctggc cggactacat
      541 gcgggtgcgg gagcgctcgc tccagcaggg gcgtttcctg ggcagcatgg acgaggcccg
      601 gcgggcccac gtggccgtca tcggccatga catcgccaac accctcttcg agggacgcaa
      661 tccgatcggc caggaaatcc gggtcaacga cgaacggttc gaagtgatcg gcgtcttcga
      721 aaaggccaag tcgaatttcg gcaacaccta cgaaaacaac gccgtcgcca tcccgctgtc
      781 caccttcgaa aagatccacc cctgggagga ggcgctcggg ctgtcggtgc gcgccgtcac
      841 cctcgacaag ctcgagcagg ccaaggaaga ggtcatcggc gccctccgcg tctaccgcaa
      901 ggtccccttc ggaaaagaga acaacttcga cctgctgacc caggacgcca tccgcgagca
      961 ggtggagaag atcaccgact acatctacat cgccatgctc gtgatcacct cggtcggact
     1021 gatggtgggg gggatcgggg tgatgaacat catgctcgtc tcggtcaccg aacgcacccg
     1081 ggagatcggg gtccgcaagg cgatcggcgc caaacggtcc aacatcctcc tgcagttcct
     1141 caccgaggcc acctccctca gcggcgcggg cggaacggcc ggcattatca tcggcgtgct
     1201 cttcggagtc gccgtcaaca gcgcgatcgg cttccccatc gccatctccg tcttctggat
     1261 cgtcgttggc ttcctggtcg ctgtctcagt ggggatgatt tccggcatgt acccggcagt
     1321 caaggcggcc aagctcgacc cgatcgaggc cctgcgctac gaataactgg tttctttctt
     1381 cccggccccg gatatttttg atcttgccgc cggggtaccg ccgtcgatac cttggaacgg
     1441 accggaaaac cccttcgttt aaagggtgat atggtgagaa ccttccttcg cctgaccgtc
     1501 ctcggacttc tggtgtgggt agtcgccgcg gccgccgctt tcgggcagtc cccgctcctg
     1561 cgccgcgcca tcaccgggga agccaagaag ctgatcgtcg gcaccggcgt ctttccgtcg
     1621 gaggagccgg gtcgggtgcg cctggaggtc tactaccagg tatacaacta cgccctcgac
     1681 tttcagcagt tgggcgggct gttccgcgcc cgctacgtga cgacgattcg ggtcaacgac
     1741 cggaaggcgg acacgacggc ggcggtgtcg cgcgaggagc gcgaggcggt tgtcgatgcg
     1801 ctggcgaaaa cgacgtcgac cgccgactac cgcgccgccc aggtcaattt cgatctgccg
     1861 cccggcgact acgaggttct cttcctgatc gaaaacgtcg gctcctcggc catcctcaag
     1921 cgggatttcg acctccgcct ggagccgttc gacagccgct ccgccgagct ctcggatatt
     1981 gaactggccc aggccgccgg gccgaacgaa tcggactcgg ccgccggcgc tttcgccaag
     2041 ggcgagttca tcgtggtccc gtcgctcacg ggctcctttt ccggcgacga cggcggccgc
     2101 ctgctctttt acctggaaat ccaccgcggg ggcgattccc tcagcgaggt gaccgtcgtc
     2161 accgccctgc gccacgagag caaggggatg gtgtaccgcg acagcctcac gaccgcgctc
     2221 ccgcagcgca tcacccgaca gctccgggag atctcgctgg acggcttcat gcccggcgat
     2281 tacaggctgg aggtgtcgct gcacgggcga cggtacaagc agctcgacca gcggcaggcc
     2341 gagttccgca tcccctggac cgccgcgagc gtcctgcgct acgactggaa cgccgcgctc
     2401 gaccagttgg agttgatcgc tgacggcaac gagatcaagc ccctgcggaa gatcgactcg
     2461 ctggccgatc gggtggccgc gctcgaccgc ttctgggcag agcgcgaccc cgatcccacc
     2521 accgccgtca acaagatcaa ggtcgtcttt tactaccgcg tcgatctcgc caaccgcctc
     2581 ttcagcattc tcaacatgga gggctggcgc accgaccggg ggcgggtgct gatccgctac
     2641 ggcgagccgg accagatcga cgactacccg ctcgt
//
LOCUS       DAOOKS010000234         2670 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_47034_length_2670_cov_2.923518, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000234 DAOOKS010000000
VERSION     DAOOKS010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47034_length_2670_cov_2.923518"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="PK112_04655"
     CDS             complement(<1..279)
                     /locus_tag="PK112_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11373.1"
                     /translation="MRLLLVLATTVVLGSTLLLSCTAESESDGPVTPPVSVLNQGDYI
                     PDIATFLQIGGCSPTAYSWDGRDVYFTSSMSGASQVYRLTREGWPHQLT"
     gene            complement(468..764)
                     /locus_tag="PK112_04660"
     CDS             complement(468..764)
                     /locus_tag="PK112_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11374.1"
                     /translation="MKERRHRLNCWEFKNCGREPGGLMADLLGQCPVAAAMKYDGVNG
                     GLGGGRICWLVDRQEGCAGGGRCGTGRCHECDFYRRVVFEEDDAATYAFRTAPT"
     gene            complement(795..>2670)
                     /locus_tag="PK112_04665"
     CDS             complement(795..>2670)
                     /locus_tag="PK112_04665"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPC11375.1"
                     /translation="GPPASTIDPDFSPIAGSEACVPEPAEDDDAWFGSQPPPEEPQLT
                     ASGKIRRLTEEEVKSIAGSLYRADAFLSDKDKKELMKKLDDKEQLFAGSAPIEPPKRP
                     EREKPKPADVLAEIENLGPSPKMAGRGRGVAYFYRNLIQLRGSQQLFAGDEIAVAGKT
                     YELRRKTIGRTPLLVGGGVAFVVILFLVATLFVSRPGREGRVAGIVLDDMQLPVNRGA
                     SVVFPELGQTIRVNGQGMFQTEALPPGSHKVEVLVNGKTFASDYVTVAGGELSLIALK
                     PMTGEEAAAAEPEAAAPSAVLGVTVPEPAPARETPGKTAAPPKAKPKAQTSTGAAGSA
                     GLKLAANVDGARLTVDGKVLGVGNLVYPQLPPGAHSYTVSREGYAPATGTITLAEGKT
                     QTLEVTLAPLSTAQKLEEYSAEDYYLSGLSAVQEQDYQAAVTDLRQAVGQEPGHADAW
                     FALGQAHAGVKDWPNAHDAFLRAAEIYRFGNDHSRAITAYNAAIRVNDKSAAAYLGRG
                     DLFLQRGDERAAAVDFETVVRLDRRNPHAYIGLGRSRMNQGMYEEAIKHFKDARSIEP
                     NNPVVYQYLMLCHMAESNFDEVRRSYAKFAETASENQMRQLQADQRYAAALRVVNGHP
                     "
BASE COUNT          414 a          886 c          884 g          486 t
ORIGIN      
        1 cgtgagctgg tgcggccagc cttcccgggt gagccggtac acctggctgg cgcccgacat
       61 gctggacgtg aagtagacgt cgcggccgtc ccagctgtac gccgtcgggc tgcagccccc
      121 gatctggagg aaggtggcga tatcggggat gtagtcgcct tggttgagga cggacaccgg
      181 cggtgtgacg ggaccgtccg actccgattc ggccgtgcag gatagaagaa gcgtgctgcc
      241 gagcaccacc gtggtcgcaa gtacgagcag aagtcgcatg cggttctcct gactagagtt
      301 cgcggtacgg gtcgttctac caggtgagag gaaaatagaa acggaatgtt cacgtggcaa
      361 cccaaaaacg cggggagggc ggggagtgca gaccggggac aaacaagcgc gggccgggaa
      421 cgtccgttcc cggcccgcgt atgctggtgg gcggcaagtt ggagcgccta tgtgggcgcc
      481 gtgcggaagg cgtaggtcgc ggcgtcgtct tcctcgaaca cgacgcggcg gtagaaatca
      541 cattcgtggc agcgtcccgt cccgcaccgt cccccgcccg cgcatccctc ttggcggtcg
      601 accagccagc agatgcgccc gccgccgagc ccgccgttga cgccgtcgta cttcattgcc
      661 gcggcgacgg ggcactggcc caggaggtcc gccatgagtc cccccggctc gcggccgcag
      721 ttcttgaact cccagcagtt gaggcggtga cgtcgttcct tcatgttctc agattccggc
      781 taagcggtgg ctcctcatgg atgtccgttg acaacccgca gcgcggcggc gtagcgctgg
      841 tccgcctgca actggcgcat ctggttctcg ctggcggtct cggcgaactt ggcgtagctc
      901 cgccggactt catcaaaatt gctctcggcc atgtggcaga gcatgaggta ctgatagaca
      961 acggggttat tcggttcaat cgagcgggcg tccttgaagt gcttgattgc ctcctcgtac
     1021 atgccctggt tcatgcgcga ccgtcccaac ccgatgtagg cgtgcggatt gcgccggtcg
     1081 agccgaacga cggtctcgaa atccaccgcc gccgcccgtt cgtcgccgcg ctggaggaag
     1141 aggtcgccgc gcccgaggta cgcggccgcc gacttgtcgt tcacccggat ggccgcattg
     1201 tacgccgtga tcgcccggct gtggtcattg ccgaaacggt aaatctccgc ggcccggaga
     1261 aaggcatcgt gggcattcgg ccagtctttc acgcccgcgt gcgcctggcc aagcgcgaac
     1321 caggcatcgg cgtgcccggg ctcctggccg accgcctggc gcaaatcggt cacggccgcc
     1381 tggtaatcct gctcctgcac ggccgacaac ccggagagat agtagtcctc cgcagaatat
     1441 tcttccagtt tctgtgccgt cgatagcggc gccagcgtca cctccagggt ttgcgtcttg
     1501 ccctcggcca gcgtgatggt cccggtcgcc ggagcgtacc cctcccggct gaccgtgtac
     1561 gagtgcgcgc ccggcggcaa ctgcgggtag acgaggttgc cgaccccgag gaccttgccg
     1621 tcgaccgtga gccgggcgcc gtcgacattc gccgcgagtt tcagtcccgc cgaaccggcc
     1681 gcgccggtcg aggtctgggc tttcggcttc gctttcggcg gagccgcggt cttgcccggc
     1741 gtctcccgag ccggcgccgg ctcagggaca gtcacgccca ggacggccga tggagccgcc
     1801 gcctcgggct ccgccgccgc tgcttcttcg ccggtcatcg gcttgagcgc gatcaggctc
     1861 agttctcctc ccgcgaccgt cacgtagtcc gaagcgaaag tcttcccgtt gacgaggacc
     1921 tcgaccttgt ggctgcccgg aggcagagcc tcggtctgga acatcccctg gccgttgacg
     1981 cggatagtct ggccgagctc cggaaagacg accgacgcgc cccggttgac cggcagctgc
     2041 atgtcgtcca ggacgatccc ggcgactcgc ccctcgcggc ccggacggga cacgaagagc
     2101 gtggcgacga ggaacaggat gacgacgaag gctacgccgc caccgaccag caggggggtg
     2161 cgcccgatcg tcttgcggcg cagttcgtag gtttttccgg ccacggcgat ttcgtcaccc
     2221 gcaaagagct gctggctgcc ccgcagttga atcaggttgc ggtagaaata ggccacccct
     2281 cggccccggc cggccatttt cggcgagggc ccgaggtttt caatctccgc cagcacatcg
     2341 gccggtttcg gcttctcccg ctccggtcgt ttcgggggct cgatcggcgc actgcccgca
     2401 aacaactgct ccttgtcatc cagcttcttc atcagttcct tcttgtcctt gtcgctgagg
     2461 aacgcgtcgg cgcggtagag actgccggcg atcgatttca cttcttcttc ggtcaaccgc
     2521 cggatcttgc cggaagccgt caactgcggt tcttcgggcg gcggctgtga accgaaccag
     2581 gcatcgtcgt cttccgccgg ttccggcaca caggcctcgg agccggcgat cggggagaag
     2641 tcggggtcga tcgtcgaggc cggcggcccg
//
LOCUS       DAOOKS010000235         2666 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_47118_length_2666_cov_4.027959, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000235 DAOOKS010000000
VERSION     DAOOKS010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47118_length_2666_cov_4.027959"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="PK112_04670"
     CDS             <1..220
                     /locus_tag="PK112_04670"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HPC11376.1"
                     /translation="WVRHPSYTGLLIAFLGLGIAYANWLSLVMLMVPITTAVVHRIVR
                     EEQALHSALGPVYTAYCARVKRLVPGLF"
     gene            340..1422
                     /locus_tag="PK112_04675"
     CDS             340..1422
                     /locus_tag="PK112_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11377.1"
                     /translation="MNVMIQPGIIRRFAVIAVLLLAGIGAIRAAGAEPPAESRLAAAD
                     IALIGEAYRLVKTLGAEVWPDFERTDAPVLYIKGERQYAVGFPTPLEGFVPLEGVTVA
                     GRAVQVAPRSFPPALAAAFPLQGIHAAVLGTPAVLELSPPQWALKAAHEMFHVFQYRH
                     GWLEKVAALEIGPRDQADWQLNFPFPYQDPDVAALMHLGSYPTCLAIGATTAADAVYN
                     TGVAAEALAVLQGVLRSKTGDEKAWRYLLFQEATEGVAKYAERRIAEAAAAAGGRPAV
                     EFSALPGCVPYGTVWTDDYAGQTFLVKHTGRVSRNRTEFYHLGLGKCLLLDRLDPAWK
                     ARYFEMGIWLPDLVDRAVRLQQESCR"
     gene            complement(1479..>2666)
                     /gene="kynU"
                     /locus_tag="PK112_04680"
     CDS             complement(1479..>2666)
                     /gene="kynU"
                     /locus_tag="PK112_04680"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011959139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HPC11378.1"
                     /translation="LAVFRRRFRLPRGVTYLDGNSLGALPAATAPRVRAVIEAEWGRD
                     LIRSWNRHGWVTLPGRLGDKIARLIGAAPGEVVVADSVSVNVFKLLAAALHLRPGRRM
                     ILSDRGNFPTDLYVAQGLARLLDGRAALRVVEEGEIAASIDADTAVVMLTQVDYRSGR
                     LHDLPGLTRRAHDAGALILWDLAHSAGVLPLDLNKAGVDLAVGCGYKYLNGGPGAPAF
                     LFVARRHQAMVAPALAGWFGHAEPFAFDSEYRPAPGIARLLCGTPPILSMAALEEGVD
                     LSLEADPVCVRAKSTALTERFIALVDQFCPSRFDLVSPREADRRGSQVSLAHPAGYAI
                     IHALIARGIIPDFRAPDILRFGFAPLYTRHIDVWNAVRHLRAVMDGSEWDRPEHHRRQ
                     TVT"
BASE COUNT          427 a          866 c          942 g          431 t
ORIGIN      
        1 ctgggtccgc catccatcat ataccgggct gctcatcgcc tttttgggtt tggggatcgc
       61 gtatgcgaat tggctcagcc tggtgatgct catggtgccc atcacgacgg ccgttgtcca
      121 ccggatcgtt cgggaggagc aggctctcca ctcggctctc ggcccggtct acaccgccta
      181 ctgcgcccgc gtgaagcggc tggttccggg cttgttctga tcatcttcct tcgataattc
      241 gggttctacg gttttgggaa agagtgccga tgaacgggtc gaaccgaacg gcggatagtg
      301 cgtaatatgg tatgaatcca cctcccggga gatcacccca tgaacgtgat gatccagccc
      361 ggtatcatcc ggcgtttcgc cgtgatcgca gttctgcttc tggccggcat cggcgcaatt
      421 cgggcagccg gtgctgaacc gccggccgaa agtcgtctgg cagcagccga cattgcgctc
      481 atcggagagg cgtaccgcct cgtgaagacc ctgggcgctg aggtctggcc tgatttcgaa
      541 cggacggacg cccccgtgct ctacatcaag ggtgagcggc agtacgccgt cggcttcccg
      601 acaccccttg aggggttcgt tccgctggag ggcgtgacgg tggccgggcg cgcggtgcag
      661 gtagcgcccc ggagcttccc gccagccctc gccgccgctt tcccgctgca gggcatccac
      721 gcggcggtgc tgggaacgcc ggcggtgctg gaactgtcgc ccccgcaatg ggcgctcaag
      781 gcggcgcacg agatgttcca cgtgttccag taccgccacg gctggctcga gaaggtggcg
      841 gcgctggaga tcgggccgcg ggaccaggct gactggcagc tcaacttccc gtttccatac
      901 caggatccgg acgttgccgc cttgatgcat ctggggtcgt atcccacctg tctcgccatc
      961 ggggcgacga cggctgccga cgcggtctac aacaccggcg tggctgccga ggcgctggcg
     1021 gtgctgcagg gcgtgctgcg gtccaagacg ggcgacgaga aagcatggcg gtaccttttg
     1081 tttcaggagg cgaccgaggg ggtggccaag tatgccgagc gccggatagc ggaagctgcg
     1141 gccgccgccg gcggcaggcc cgcggtggag ttcagcgccc tgccggggtg cgtgccatac
     1201 gggacggttt ggacggacga ctatgccggt cagacgttct tggtgaagca taccgggcgg
     1261 gtgtcgagga atcggaccga gttctaccac ctgggcctcg gcaagtgcct gctgctggac
     1321 cggctcgacc cggcatggaa ggcgcgctac tttgagatgg ggatctggct gcccgacctc
     1381 gtggaccggg ccgtgcgcct gcagcaggag tcctgccggt gaccggaggt gacgatcgaa
     1441 gtcgccgccg gacttcagcg ggataaaacc ggaatctgtt aagtgaccgt ctgacgccgg
     1501 tggtgttccg ggcggtccca ttcgctgccg tccatcacgg cccgcaggtg acgaaccgcg
     1561 ttccagacgt cgatgtgccg cgtgtacaga ggggcgaaac cgaagcgcag gatgtccggc
     1621 gcccggaagt cggggatgat cccgcgggcg atcagcgcat gtatgatggc gtagccggcc
     1681 ggatgagcca ggctgacctg gctgccccgc cggtccgcct cgcgcgggct gaccaggtcg
     1741 aaacgactcg ggcagaactg gtccaccagg gcgatgaagc gctccgtcag ggcggtggac
     1801 ttggcgcgca cgcacaccgg gtcggcttcc agggacagat cgacgccctc ttcgagtgcg
     1861 gccatgctca ggatcggcgg cgtgccgcag agcagccgcg cgatgcccgg agcgggtcgg
     1921 tactccgagt caaaggcgaa cggctcggca tgcccgaacc acccggccag cgccggcgcc
     1981 accatggctt gatgccgtcg ggcgacaaag agaaaggcag gcgccccggg tccgccgttg
     2041 aggtacttgt agccgcagcc caccgccaga tccactcccg ccttgttgag gtccagcggc
     2101 aggacgccgg ccgaatgggc cagatcccag agaatcagcg cgccggcgtc gtgggcgcgg
     2161 cgggtcagcc cgggcaggtc gtgcagccgg ccgctgcggt agtcgacctg cgtgagcatc
     2221 accaccgccg tgtccgcatc gatcgacgcg gcgatttcgc cttcctccac cacccgcagc
     2281 gcggcgcgac cgtccagcag gcgggccagg ccctgggcga cgtacaggtc ggtggggaaa
     2341 ttgccccggt ccgacaggat catccggcgg ccgggccgca ggtgcagcgc cgccgccagc
     2401 agcttgaaca cattgaccga cacggagtcg gccaccacca cctcacctgg tgcggcgccg
     2461 atgagccggg cgattttgtc gccgaggcgc cccgggagtg tcacccagcc atgccggttc
     2521 caggagcgga tcaggtcccg gccccactcg gcctcgatca cggcgcgcac ccggggggcg
     2581 gtggccgccg gcagagcgcc gagcgaattg ccgtcaaggt aggtcacgcc gcggggaagg
     2641 cggaagcggc ggcggaacac cgccag
//
LOCUS       DAOOKS010000236         2664 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_47174_length_2664_cov_3.060943, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000236 DAOOKS010000000
VERSION     DAOOKS010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47174_length_2664_cov_3.060943"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="PK112_04685"
     CDS             <1..447
                     /locus_tag="PK112_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HPC11379.1"
                     /translation="CTSHRARPSSSARLLAEMTDGRYSMVELDEKYNLQVLDDGQFYG
                     IDRFSGGEKDLANLCLRLAISEALLQSAGIDRSFIILDEVFGSQDDGRKELIVQALTN
                     LTHRFPQIFLITHVGDIRDRVECLIEVAPTGLGWSKVTVRGEETEA"
     gene            425..1087
                     /locus_tag="PK112_04690"
     CDS             425..1087
                     /locus_tag="PK112_04690"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HPC11380.1"
                     /translation="MARKRKHKARLAGRLALLLVLIAAVLIVRRSDIGRDPAPVPLTT
                     VVKIIDGDTMVLGNGETVRLLAIDTPEDGEPLHDEAAALLAQVALGKEARIVSSGRRR
                     DTYGRLLAYVYVDDTLLVNRVLVDSGLAYVYLFRDTDTGRPETDRIIAAQRAALDRRA
                     GLFALPRAAEPYYVAGRTSYRFHRPGCPSTAEFTSGRARTFATRRDALAEGLSPCRRC
                     KP"
     gene            1239..>2664
                     /locus_tag="PK112_04695"
     CDS             1239..>2664
                     /locus_tag="PK112_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11381.1"
                     /translation="MYSYQEEVFMSFRTILPVLSADAVLLAAATMADAPRVISFQGRL
                     LDDAGAPLPDGPKSIRIIIWNDPASTLPSHEVWNSGPLTVETAGGLFSLNLGETPMPP
                     LGPGLLADSGLWIGITVGAEAEGSPRIKMTSSAFAFRATLADTAAYADEASYAGEAAH
                     ADEANRAGAAVQADSAGYADTAAYAFSAPVSAVDHGSLAGLADDDHPQYLRADGGRMM
                     TGDLDLGKHQIHSLEAGTTSSDAVRYDQAVKDGDAAGGDLTGTYPAPQLAADAVGTAE
                     IAAGAVGTSEVADNSLTATDLASSSVSTAEVTNNSLLHDDLATGAVRSDEIQDGAIVN
                     ADINAAAAIAPSKIAGTACDLATDQAITGNKTFRAATYFYDSTMAVGSYSVLIGTDSI
                     TPQWTYPLQVSRKYSTSNVTHGIDVHLRNEGIGNIWGYYSRVGVNDASSGTRSGIQVT
                     AGDSSNTVGTSYGVRTYAYGGDQTY"
BASE COUNT          455 a          926 c          844 g          439 t
ORIGIN      
        1 tgtacttctc atcgagctcg accatcgagt agcgcccgcc tgctggccga gatgaccgac
       61 gggcgctact cgatggtcga gctcgatgag aagtacaacc tgcaggtgct cgacgacggg
      121 cagttttacg gcatcgaccg cttctccggc ggcgaaaagg atctggccaa cctctgcctc
      181 cgcctggcga tctcggaggc gctgctccag tcagccggca tcgaccgctc cttcattatc
      241 ctcgacgagg tgttcgggtc gcaggacgac gggcgcaagg agctgatcgt acaggcgctc
      301 accaacctca ctcaccgttt tccccagata ttcctcatca cccacgtggg cgatatccgg
      361 gaccgcgtcg agtgcctgat cgaggttgcg cccaccgggc tggggtggtc gaaggtgacg
      421 gtgcgtggcg aggaaacgga agcataaagc ccggctggcc ggacggctgg cgctcctttt
      481 ggtcctgatc gcggcggtcc tgatagtccg ccgctcggac atcggccgcg acccggcgcc
      541 ggtccccctc accacggtgg tgaagataat cgacggcgac accatggtgc tcggcaacgg
      601 cgagacggtc cgcctgctgg caatcgacac gcccgaagac ggggagccgc tccacgacga
      661 ggcggccgcg ctgctcgcgc aagtcgctct cggcaaagaa gcccgcatcg tttcttccgg
      721 ccgccggcgc gacacctacg gccgcctcct ggcgtacgtg tacgtcgacg ataccctgct
      781 ggtgaaccgg gtgcttgtcg acagcggcct cgcttacgtc tacctgttcc gggacaccga
      841 caccgggcgc cccgagaccg accggatcat cgccgcccag cgagccgcgc tcgaccgccg
      901 ggcggggctg ttcgcgctcc cgcgggccgc ggagccctac tatgtcgccg gccgaacctc
      961 ctaccgcttc catcgtcccg gctgcccctc gaccgccgaa ttcacgtccg gccgggcccg
     1021 cacttttgcg acccgccgcg acgccctggc cgagggactc tcgccatgcc gtcgctgcaa
     1081 accctgagcg ccgcgcccgg acggccgtta aggccgcgga ccaatcggtc gattatcgcc
     1141 attgacagcc tctgtaagga gactatcttt ctcttggcgg cgggttctcc cgcaacccgc
     1201 ccgccgcgct tcgtccgatg caggaaacca gagctgtagt gtatagctat caggaggagg
     1261 tcttcatgtc ttttcgcacg atcctgccgg tgctgtcggc ggacgcggtt ctgctggcgg
     1321 ccgcgacgat ggccgacgcc ccccgcgtga tttccttcca gggccggctg ctcgacgacg
     1381 ccggggcgcc gctgcccgac ggcccgaaga gcatccgcat catcatctgg aacgaccccg
     1441 cctccaccct cccctcccac gaagtctgga actccggtcc gctgacggtg gagacggcgg
     1501 gcgggctgtt ttcgctcaat ctcggagaaa cgccgatgcc gccgctcggg ccggggttgt
     1561 tggccgacag cgggctctgg atcgggatca cggtcggggc ggaggccgag ggatccccgc
     1621 gcatcaagat gacttcatcg gctttcgcct tccgcgccac ccttgccgat acagccgcat
     1681 acgccgacga ggcgagctat gccggtgaag ctgcccatgc tgacgaagcc aaccgtgccg
     1741 gcgccgcggt tcaagcggac agtgctgggt atgccgacac ggccgcgtac gccttttccg
     1801 cgcctgtatc ggccgtcgac cacgggagcc tggccgggct cgccgatgat gaccacccgc
     1861 agtacctgcg cgccgacggc ggccgaatga tgaccgggga tctcgacctc ggaaagcacc
     1921 agatccacag tctggaggcg gggaccacgt catcggacgc cgtgcgctat gaccaggcgg
     1981 tgaaggatgg ggatgccgcg ggcggcgacc tgaccggaac ctatccggcc ccccagttgg
     2041 cggccgatgc ggtgggtacc gcggagatcg ccgccggcgc cgtcggcacc agcgaggtgg
     2101 ccgacaactc ccttaccgcc accgacctgg cgagcagctc ggtcagtacg gccgaagtaa
     2161 caaacaactc cctgctccac gatgacctcg ccaccggagc ggtccgcagc gatgaaatcc
     2221 aggacggggc gatcgtcaac gccgatatca acgcggcggc cgccatcgcc ccgtccaaga
     2281 tcgccggaac ggcctgtgat cttgcgacgg accaggccat cacggggaac aagacctttc
     2341 gggccgcgac gtatttctat gacagcacga tggcggtcgg cagttactcc gttttgatcg
     2401 ggaccgattc catcacgccg cagtggacat acccgctgca ggtaagccgg aaatactcca
     2461 cttcgaacgt gacccacggt atcgatgtcc acctgcgcaa cgagggaatc gggaacatct
     2521 ggggctacta ttcccgggtt ggcgtcaacg acgccagctc cggtacgcgc agcggaattc
     2581 aggtcacggc gggtgattcc tccaacactg tgggtaccag ttacggcgtg cgcacctacg
     2641 cctacggcgg ggaccagacg tacg
//
LOCUS       DAOOKS010000237         2659 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_47315_length_2659_cov_2.629800, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000237 DAOOKS010000000
VERSION     DAOOKS010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47315_length_2659_cov_2.629800"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1222)
                     /locus_tag="PK112_04700"
     CDS             complement(<1..1222)
                     /locus_tag="PK112_04700"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HPC11382.1"
                     /translation="MKILVLNAGSSSVKYQLIDSETEKALAKGMVSRIGMSGSLLAHK
                     PFDRPEVKVSGEILDHIQAVEYIVTMLLSPNHGVIKDRAEIGAVGHRVVHGGEKFTDS
                     VLITPALMQELRNLIELAPLHNPHNIRGITACERTLPGLPQVAVFDTAFHYTMPGYAY
                     IYGIPYVLYKRYGIRRYGFHGTSHKFVSDRAAVMLRRPLAELRVVTAHLGNGASITAV
                     DRGVSVDTSMGFTPLEGLLMGTRSGDMDPAIILHLMAREELSLHEANTLLNKHSGLIG
                     ISGVSSDMREIQSASQAGNANAQLALDIYCYRIKKYVGAYAAAMGGIDALIFTGGVGE
                     NSAVVRAKVCEGLAFLGLQVDPEKNSAAHAAFTSARRCSRSTQASRSCETAKNKEWSS
                     NDESPLRPWISRMPK"
     gene            complement(1235..1603)
                     /locus_tag="PK112_04705"
     CDS             complement(1235..1603)
                     /locus_tag="PK112_04705"
                     /inference="COORDINATES: protein motif:HMM:NF012928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HPC11383.1"
                     /translation="MTVNEYPGYVTTRIIVPLLNEAMHVLMEGVASADDIDRAMKLGF
                     GFNMGPLALADVMGLDVVMKWMENLLDELAEHKYNPCPLLRKMVRSGHLGVKTGRGFF
                     VYDNEGNRVPNPAAAAAVTK"
     gene            complement(1601..2167)
                     /locus_tag="PK112_04710"
                     /pseudo
     CDS             complement(1601..2167)
                     /locus_tag="PK112_04710"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
     gene            2393..>2659
                     /locus_tag="PK112_04715"
     CDS             2393..>2659
                     /locus_tag="PK112_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11384.1"
                     /translation="MKQAIPFILLLLLAIGLIAAQPAEAAIQRVAARYNMVDVYGGYA
                     SPHGEYGEVGLVMFIDEFDRPVEVDADQVFDAGWSLGFNYGTLYA"
BASE COUNT          421 a          685 c         1006 g          547 t
ORIGIN      
        1 acttcggcat tcgcgagatc cagggacgga gaggagactc gtcgttgctc gaccattcct
       61 tgttctttgc cgtctcgcag gacctgctcg cctgcgtcga ccgggagcac cgcctcgccg
      121 aagtgaacgc ggcgtgggcg gcgctgttct tttccgggtc gacctggagg ccgaggaagg
      181 cgagtccctc gcagactttt gcgcgcacga cggcggagtt ttcgccgacg ccgccggtga
      241 agatgagggc gtcgatcccg cccatggcgg ccgcgtatgc gccgacgtac ttcttgatcc
      301 ggtagcagta gatgtcgagg gcgagctggg cgttggcgtt gccggcctgg gaggccgact
      361 ggatctcgcg catgtcggag gagacgccgg agatgccgat gagcccggag tgtttgttca
      421 gcagggtgtt ggcctcgtgg agggagagtt cctcgcgggc catgaggtgg aggatgatcg
      481 ccgggtccat gtcgccggag cgggtgccca tgagcagccc ctccaggggg gtgaagccca
      541 tcgaggtgtc gaccgagacg ccgcggtcga cggcggtgat ggaggcgccg ttgccgaggt
      601 gggcggtgac gactctcaat tcggcgagcg ggcggcggag catgacggcg gcgcggtcgc
      661 tcacgaactt gtgggaggtg ccgtggaagc cgtagcggcg gatgccgtag cgcttgtaca
      721 ggacgtaggg gatgccgtag atgtaggcgt agccgggcat ggtgtagtgg aaggcggtgt
      781 cgaagacggc gacctggggg agtccgggga gggtgcgctc gcaggcggtg atgccgcgga
      841 tgttgtgggg gttgtggagg ggggcgagtt cgatgaggtt gcgcagttcc tgcatgaggg
      901 cgggggtgat gagcaccgag tcggtgaatt tctcgccgcc gtggacgacg cggtggccga
      961 cggcgccgat ctcggcgcgg tccttgatga cgccgtggtt gggcgacagg agcatggtga
     1021 cgatgtattc gacggcctgg atgtggtcga ggatctcgcc ggagactttc acctcggggc
     1081 ggtcgaacgg tttgtgggcg aggagggatc cggacatgcc gatccgcgag accattcctt
     1141 tggccagggc tttttccgtc tccgagtcga tgagctggta tttcacggag gacgacccgg
     1201 cgttgagcac cagtatcttc atggcggacg ttccttattt tgtcacggca gccgcggcgg
     1261 ccgggttggg gacgcggttg ccctcgttgt catagacgaa gaagccgcgt ccggttttta
     1321 cgccgaggtg gccgctgcgg accatcttgc gcaggagggg gcaggggttg tacttgtgct
     1381 cggcgagttc gtcgagcagg ttctccatcc acttcatgac gacgtcgagt cccatcacat
     1441 cggccagggc gagggggccc atgttgaagc cgaaaccgag tttcatggcc cggtcaatgt
     1501 cgtcggccga agcgacgcct tccatgagca cgtgcatggc ctcgttcagc agggggacga
     1561 tgatgcgggt ggtgacgtac cccgggtact cgttgaccgt gatgtactcc ttgtcgagga
     1621 gctgggcgaa ggcgacggct ttgcggaagg tgtcgtcatc ggtgtggaag cccttgacga
     1681 tctcgacgac ggggaccttg gtcaccgggg tgaggaagtg catgccgatg attttctggg
     1741 ggcgttcggt gacggcggcg atttcggaga cggagatggt gccggtgttg gtgaccaaga
     1801 gggcgtcggg acggacgacg gtgtcgagtt tttcgaagag gtcgcgcttc atctggaggt
     1861 cttcggggat cgcctcgagg atgatttcgg cgtcctcggt catggagagg tcggaggagg
     1921 ggtagatgcg ggcgaggatg gctttcttgt cggactcggt gaggccccat ttgccgatct
     1981 cccggttcat ggcttcggcg atggctttga cgccgcgttc ggcgagccgg gtggttttgt
     2041 cgatcagggt gacttcggtg ccggcggcgg cgacggcctc ggcgaggccc tggcccatca
     2101 caccggcgcc gatgattgct atcttttttc cggccatgtc attagtccta tgtgtcaggt
     2161 cttcaagtct atcggttttg tcgacgaagg agtcgacaaa tcaccccgaa tataattgct
     2221 ctttcctgca gggcaaccgc aaattatact gcgggaggta tgatcggccc cgccgctcgg
     2281 cggaagcggc cccggcgtgt acaagggagg agacaaaccg ggtccggctc ggcggcgtcg
     2341 agccgggcgc accgggtggg cggccgcgaa gatccagacg gaaggagcag ctatgaagca
     2401 ggcgataccc tttatcttgt tgttactctt ggcgataggt ctcattgccg cacagccggc
     2461 cgaggcggcg atacagcggg tcgcggcccg ctacaatatg gtggatgtct acggggggta
     2521 tgcgagcccg cacggagagt atggcgaggt cgggctggta atgtttattg acgagtttga
     2581 ccggccggtg gaggttgacg cggatcaggt gttcgacgcc ggctggtctt tgggcttcaa
     2641 ttacggtact ctgtacgcc
//
LOCUS       DAOOKS010000238         2656 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_47370_length_2656_cov_3.617455, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000238 DAOOKS010000000
VERSION     DAOOKS010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47370_length_2656_cov_3.617455"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2656)
                     /locus_tag="PK112_04720"
     CDS             complement(<1..>2656)
                     /locus_tag="PK112_04720"
                     /inference="COORDINATES: protein motif:HMM:NF019368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HPC11385.1"
                     /translation="GFDREDVVEAAVHFGDRKTRFVRVFGPDGREAPADAVPMAGDSL
                     RLRFLARVPSVGYAVFDARPADAPCPLATGLRVSPRELENERYRVGLNEKGEVASIYD
                     KKLGRELLGAPVRYELLEDTPHNWPAWEVDYEDVMAPARDEWAGDPVIAVVEEGCARV
                     AVSIERHTASSTLRTIVSLAAGEAGERVEFQAAIDWYERERMLKLAVSPATANETVTY
                     DLGLGVIERGRNSEKRYEVPGHQWADLAAPAGDWGVAVLNDCRYGWDHPDSATVRLTL
                     VRTPGVGKGWDWVGDQRSQDLGRHLLGFALQGHAGDWRSGGVVRQGARFNQPLQAFRA
                     QPHEGELGKTYSLVRAEETPARGGAATEAPGVMIAAVKMAEECDEIIIRVRETAGAPR
                     TQAALAFDRPIAAAREVNGAEENVGPAEVADGRLVVSLTAFQPKAFAVTLDEGVVTDL
                     LLPWYSPLELPYDGDGVSTDDERCGGDFDGRGYSLAGELLPDTLDYLDIPFVLGPRAA
                     GASNMLTCRGQQVPLPGLGENRLWLLAAAVDGPAVDTFRISGRALPVELPDYAAPLGQ
                     WHNRVVDGQLVESPSAIAPAYLTAAPVAWYGSHRHTPECGNDIYRFTYAFLVSLEVLP
                     GATAVALPNNARIRVLAATVGAKPHGDVTPARPLYDETRATVVKVAADSGAFAGRCRL
                     TLSSPTPGAAIYYTLDGTEPSVRSARYTAPVAVDRTTTLRARAVRDGYDNSYVAERTV
                     HRLELRAPVSVEGLAAGLTARYYEGEWERLPRFDTLTAAAEFTAETVALPPLARQEDF
                     GLLFEGYIRVPADGMYAFSINSDDGSRLYVADTLVVDNDGLHGDTELSGLIGLKAGLH
                     RFTVQMFQGKGGSGLGVSIAGPSLAKQAL"
BASE COUNT          428 a          987 c          856 g          385 t
ORIGIN      
        1 aagagcctgc ttggccagcg acggaccggc gatcgaaacc cccagcccgg atcccccctt
       61 gccctgaaac atctgcaccg tgaagcggtg aagcccggct ttcaaaccga tcaacccgct
      121 caactcggtg tcgccgtgca aaccgtcgtt gtcgaccacg agcgtgtccg ccacgtacag
      181 ccggctgccg tcgtcggagt tgatcgaaaa cgcatacata ccgtcggccg gcacccggat
      241 atatccctca aagagcagac cgaaatcttc ctggcgggcg agcggcggga gagcaacggt
      301 ctcggccgta aattcggccg cggccgtcag cgtatcgaat cgcggcaggc gttcccattc
      361 gccttcgtag tagcgcgctg tcagcccggc ggccagcccc tcaaccgaca ccggcgcgcg
      421 cagttccagg cggtgcacgg tgcgctccgc gacgtacgaa ttgtcgtacc cgtcccgcac
      481 cgcccgcgcc cgcagagtgg tcgtgcggtc aacggccacc ggcgcggtgt agcgcgcgga
      541 acgtaccgag ggctccgtgc cgtccagcgt gtagtagatc gcggccccgg gagtcggcga
      601 actcagagtc aaccggcatc ggccggcgaa cgcgccgctg tcggccgcga ccttcactac
      661 cgtggcgcgg gtttcatcgt acagcggccg ggccggcgtc acgtcgccat gcggcttggc
      721 ccccacggtc gcggccagca cccggatgcg cgcgttgttc ggcagcgcca ccgcggtcgc
      781 ccccggcaga acctccagcg aaaccaaaaa ggcataggtg aagcggtaga tgtcgttgcc
      841 gcactccggg gtgtggcggt gcgagccgta ccacgccacg ggcgccgcag tgagataggc
      901 cggggcgatc gccgagggag actcaaccaa ctgcccatcg accacccggt tgtgccactg
      961 cccgagcggc gcggcgtaat ccggcagctc caccggaagg gcgcggccgc tgatccgaaa
     1021 cgtgtccacg gccgggccgt ccacggccgc cgcgagcagc cagagacggt tctccccgag
     1081 tcccggcaag ggcacttgct ggccgcggca ggtgagcatg ttggacgctc ccgccgcgcg
     1141 cgggccgagc acaaagggaa tgtcaagata gtcgagagtg tcgggcagga gctccccggc
     1201 cagcgaatac ccgcgaccat cgaagtcgcc gccgcaccgc tcgtcatcgg tgctgacccc
     1261 gtcgccgtcg tacggcagtt cgagcggaga ataccaggga aggagcaggt cggtgacgac
     1321 tccttcatca agcgtgactg cgaacgcttt cggctgaaat gccgtgagcg acacgaccag
     1381 ccggccgtcg gccacctcgg cgggcccgac attctcctcc gcgccgttca cttcgcgcgc
     1441 ggccgcgatc ggtcggtcga aggcgagtgc cgcctgcgtc cgcggggcgc ccgcggtttc
     1501 ccgaacgcgg atgatgatct cgtcgcactc ctcggccatc ttcaccgcgg cgatcatcac
     1561 gcccggcgct tcggtcgctg cgccgccccg cgccggagtc tcctccgccc ggaccagcga
     1621 gtaggtcttc cccaactccc cttcgtgcgg ctgcgcccgg aaagcctgca gcggctggtt
     1681 gaagcgcgcc ccctgccgga cgacgcctcc ggagcgccag tcgccggcgt gtccctgaag
     1741 ggcgaatccc aggaggtggc gcccgagatc ctgggagcgc tgatcgccca cccagtccca
     1801 gcctttgccc acgcccgggg tgcgcacgag ggtgaggcgc acggtcgccg agtcggggtg
     1861 gtcccagccg tagcggcagt cgttcaggac cgccaccccc cagtcgcccg caggggcggc
     1921 caggtcggcc cactggtgcc ccggcacttc gtagcgcttt tcggaattgc ggccgcgttc
     1981 gatcacgccc agccccaggt cgtaggtcac ggtttcgttg gcggtcgccg gcgacacggc
     2041 gagcttgagc atccgctccc gctcatacca gtcgatcgcc gcctggaact cgacccgctc
     2101 cccggcctcc cccgccgcca gactgacgat cgtgcggagc gtggaagacg ccgtgtgccg
     2161 ctcgatactg accgccaccc gcgcgcatcc ttcctcgacc accgcgatga ccggatcgcc
     2221 cgcccattcg tcgcgggccg gagccatgac atcctcgtag tccacctccc acgccggcca
     2281 attgtgcggc gtgtcctcca gcagttcgta gcgcaccggc gccccgagca gctcccgacc
     2341 gagcttcttg tcgtagatcg acgccacctc tcccttctca ttcaacccca cccggtagcg
     2401 ttcgttctcc agttcccggg gcgagacccg caagccggtc gccagcgggc agggggcgtc
     2461 ggccggccgc gcatcgaaga cggcgtaacc caccgagggc acccgggcga gaaaacgaag
     2521 gcgcagcgaa tcgccggcca tcgggaccgc atccgccggc gcctcccggc cgtccggacc
     2581 gaagacgcga acgaaccgcg tcttccggtc gccgaagtgc acggcggctt cgacgacatc
     2641 ttcgcggtca aaaccc
//
LOCUS       DAOOKS010000239         2653 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_47442_length_2653_cov_3.393380, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000239 DAOOKS010000000
VERSION     DAOOKS010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47442_length_2653_cov_3.393380"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="PK112_04725"
     CDS             <1..332
                     /locus_tag="PK112_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019948947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPC11386.1"
                     /translation="YDELLRDLPVTTPPVDPEVEPVYHLYVIETERRDALQAFLAERG
                     VPTLIHYPVPVHLQRGYDFLGRRKGDFPVAERQAEQILSLPIFPEMTEAQVDYVAAQI
                     QAFFHGA"
     gene            322..2511
                     /locus_tag="PK112_04730"
     CDS             322..2511
                     /locus_tag="PK112_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11387.1"
                     /translation="MERNAGFSPRLEVIAALALLALVALLRSYQLGADPPLGLSISTG
                     EQTDPPQYTLFARNYVQTGDFDPFDDARRAVFLKSSVTALAVAVFSLLGAGLWQAHLV
                     GLLLGLAALVLFWLFMRKVAGPLAGLLFLFLAAFNYNLIFFGRLPFLEQALVFWAFLA
                     AALLAYGRRPAIAAAAGASLALGIYFGKAIGLVFLFPFACWFAYEILVGRRERGRWLL
                     RLAAFAAGFLVVLAGWYLWVFAPAQAQVAGYYSEQIVDLYGAPEGLQSVDDFFFKLVT
                     FGATTRLFPRMRSAALLSAAFLLIMLFHVTRRTSWREGFGRLAAGHVFVAAMIVAFYG
                     TLMIWNYRPLRYQVVLIYPIYGAAAVVLAGLWRPWGVPSRRSVPLLFYPLAVPIVMVP
                     VYQIWDSLSEHYGGGLEWADARVWVVAASAVIVAAIALGLRFRPFGGRPGTQTAARAL
                     VGAVLLGTTVLGISNYHNWSVRPTFTARDVSRDLAMTLGPGAVVSGPYAPLLAAENNL
                     PVVVHMFGVAKADPGLFGRFPITHLLVDEANEAKARADYPAIMDSAAHVVTYHVGTDQ
                     VRLYRIAGRTPNPAANAYRPSLLERAIDAYHGGRTGEGHAWAAQFLERHPRNLSAYLA
                     VGEIADLEGQFDLAEESLKGALAFSPTSYNLNARLAELYRERFDETGDPELRHKGLLL
                     YEEAIRLAPSVAKLKSACNQLKTGRTEPEEEVDSPAPAGADSANLEN"
BASE COUNT          347 a          940 c          846 g          520 t
ORIGIN      
        1 gctacgacga gctgctccgg gatctcccgg tgaccacccc cccggtcgat cccgaggtcg
       61 agccggtcta ccacctttac gtgatcgaaa ccgagcgccg cgacgccctt caggccttcc
      121 tcgccgagcg gggcgtcccg actctcattc actacccggt gccggtgcac ctgcagcgcg
      181 gctatgactt cctcggccgg cgcaagggag atttcccggt cgccgagcgc caggcggagc
      241 agatcctgtc gctcccgatc tttccggaga tgacggaggc gcaggtggat tacgtcgccg
      301 cgcagataca agcgttcttc catggagcgt aacgccggtt tcagtccgag gctcgaggtg
      361 atcgcggcgc tggccctgct cgcgctggtg gccctcctgc gcagctacca gttgggggcc
      421 gacccgccgc tcggcctgtc gatatccacc ggcgagcaga ccgacccgcc ccagtacacg
      481 ctgttcgcgc ggaactatgt ccagaccggg gatttcgatc ctttcgatga tgcccgccgg
      541 gcggttttcc tgaaatcgag cgtcaccgcc ctcgccgtcg cggtcttctc cctcctcggc
      601 gccgggctgt ggcaggcgca tctggtcggc ctgctcctgg ggctggccgc tctcgtgttg
      661 ttctggctct tcatgcggaa agtcgccggc ccgctcgccg gcctgctctt cctctttctc
      721 gcggccttca actacaacct cattttcttc gggcggctgc cctttctgga acaggcgctg
      781 gtgttttggg cgtttctcgc ggccgcgctt ctggcctacg gccggcgacc ggccatcgcc
      841 gccgccgccg gcgcctcgct cgcactcggc atttacttcg gcaaagcgat cggcctcgtc
      901 ttcctctttc ccttcgcctg ctggttcgcc tacgagatcc tcgtcggccg gcgggaacgg
      961 gggcgatggc tgctccggct ggccgctttt gcggccggtt tcctcgtcgt gctcgccggt
     1021 tggtacctct gggtgtttgc ccccgcccag gcgcaggtgg ccggctacta cagcgagcag
     1081 atcgtggatc tctacggggc gcccgagggg ctgcagtcgg tcgacgattt cttcttcaag
     1141 ctcgtgactt tcggcgccac cacccggctt ttcccgcgca tgcggagcgc ggcgctgctc
     1201 tccgcggcgt tcctgttgat catgctgttc cacgtcaccc gccggacctc atggcgcgag
     1261 ggattcggcc ggctcgccgc cgggcacgtc ttcgtcgccg cgatgatcgt ggccttctac
     1321 ggcacgctca tgatctggaa ctaccgcccc ctccgctacc aggtggtgct catctacccg
     1381 atttacgggg cggcggccgt ggtgctggcg ggcctctggc ggccgtgggg ggtgccctcg
     1441 cggcggtcgg tcccgctgct cttctatccg ctcgccgttc cgatcgtcat ggtgccggtg
     1501 tatcagatct gggacagttt gtcggagcac tatggcgggg gcctcgaatg ggccgacgcg
     1561 cgggtgtggg tggtcgccgc gtccgccgtg attgtcgccg ccatcgctct cggccttcgc
     1621 ttccgcccgt tcggcgggcg gcccgggact cagacagccg cccgggcgct ggtgggggcg
     1681 gtgctgctcg gcaccaccgt gctcggcatc agtaactacc acaactggag cgtgcggccg
     1741 accttcaccg cccgcgacgt gagccgtgat ttggcgatga ccctcggccc cggcgcggtg
     1801 gtgtccggcc cctacgcccc cctcctggcc gcggagaaca acctccccgt ggtcgtccat
     1861 atgttcggtg tggccaaggc cgaccccggc ctgttcggcc gtttcccgat cactcatctc
     1921 ctggtcgatg aggccaacga ggcaaaagcg cgggccgatt atcccgcgat catggacagc
     1981 gccgcccatg tcgtcaccta ccacgtgggg accgatcagg tgcgcctcta tcggatcgct
     2041 ggccgtaccc ccaatcccgc cgcgaacgcc taccggccct cgcttctgga gcgggcgatc
     2101 gacgcctacc acggcgggcg gacgggggag gggcacgcgt gggccgccca gttcctcgaa
     2161 cgccatcccc ggaacctctc cgcctacctc gcggtggggg agatcgccga ccttgaggga
     2221 caattcgatc tggccgagga gagcctcaag ggcgctctgg cgttttcacc taccagctac
     2281 aacctcaacg cccgcctggc cgagctctac cgggaacggt tcgatgaaac cggcgacccc
     2341 gagttgcggc acaagggcct cctgctatac gaggaagcca tccgcctggc cccgtcggtc
     2401 gccaagctca agtccgcctg caatcagttg aaaaccggcc ggaccgagcc cgaggaggag
     2461 gtcgactccc cggctccggc cggcgccgac tcggcgaacc tcgaaaatta gcgttgagtt
     2521 tcaccggccg attggcgatg ttttgagtat gcaggatgct ttactcaggt tgggagatcg
     2581 cgcacccggg accgccttca gccggtagcc cgcggtgggg gttagtgtat gccgaaaccg
     2641 tcagctcgct ttc
//
LOCUS       DAOOKS010000240         2651 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_47487_length_2651_cov_3.752311, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000240 DAOOKS010000000
VERSION     DAOOKS010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47487_length_2651_cov_3.752311"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="PK112_04735"
     CDS             <1..620
                     /locus_tag="PK112_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HPC11388.1"
                     /translation="GYLMGINPFDEPNVTESKRNTERILDQYRESGQFADLAAGRTYG
                     RLTLVSHGGESHLRPSDLADLKRLLTRFLAGLRPPEYVALLCYSKEERALDRQFAGMR
                     AAIRRRRGVATLCGYGPRYLHSIGQLYKGGPDVGRFIVFLRADYDRLEIPGRFYDFGQ
                     LITAQAIGDVRALASRDLPVLVFAVKGNVTTAVKEFADVLHAVCQ"
     gene            634..2571
                     /locus_tag="PK112_04740"
     CDS             634..2571
                     /locus_tag="PK112_04740"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF004556.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HPC11389.1"
                     /translation="MKDFDPWRGFTSSALSPEWRKKLSETARRCRAQILKMTSLAASG
                     HPGGSMSSLELYLTLYHMARVDPRNPMRDDRDRIIVSHGHTSPGAYTALAAAGFFDPA
                     AAMHGFRQAGSPFEGHVERSVPGIEWDTGNLGQGLSVGIGKALYARLSGLNFHTFVIM
                     GDGEQQKGQVGEARRVAAKFALSRLTAIIDRNRLQISGRTVDILPQDLAAEWEADGWQ
                     AVEIDGHSFDQIYGELHRAVTNEGRPIVIIANTVMGKDVGFMENDEAFHGAPVAPDML
                     TPALALLGDHGKDLPALRELRKQGPPPAFKIPRPPYPPVETGQPATYGADVKMDNRTA
                     FGKALLAVADLNLPREDFVMAVFDCDLAGSVKTAAFGQKYPDNFFQCGITEHNTAAIA
                     GSLSAERAVSIWADFAVFGVDETFNQARLNDINHANLKLFCTHTGVQVGEDGKTHQCI
                     DYFALLNSTFGWRVITPADPNQTDRITRWVLSRPGNFAVFMGRSATPIIPDADGRPFF
                     GGDYEYRYGRMDVLRRGERLALVAAGNMCAIALDAWQRLSREGVGISLISVADWSDFH
                     ADDLQMLAAHEHLVVLEDHNVKTGLGTALAAELFQAGARSTLTKMGVTAYASSGPPAD
                     LFRLLGLDPASVVARVKAILG"
BASE COUNT          426 a          990 c          794 g          441 t
ORIGIN      
        1 ccggctatct catggggatc aacccgttcg atgaacccaa cgtcaccgag agcaaacgca
       61 acaccgaacg catcctggac caatatcgcg agtcgggcca gttcgccgac ctggccgcgg
      121 gccggaccta cggccgtctc acgctcgtga gccacggggg cgagagtcac ctccgcccgt
      181 cggacctggc cgatttgaaa cggctgctca cccgattcct ggccggcctc cggccgcccg
      241 aatatgtcgc gctgctctgc tacagcaagg aagagcgcgc tctggatcgc cagttcgcgg
      301 gcatgcgggc ggccatccgg cgccgccggg gggtcgcgac cctgtgcggc tacggaccgc
      361 gctacctcca ttccatcggc cagctctaca agggcgggcc cgatgtcggc cggttcatcg
      421 tcttcctccg ggccgattat gaccggctcg agattcccgg gcggttctac gatttcgggc
      481 agctcatcac ggcccaggcg atcggcgatg tgcgcgccct ggcgagtcgc gacctccccg
      541 tgctcgtctt cgccgtcaag ggaaacgtca ccactgcggt aaaggagttc gccgatgtcc
      601 tccatgcggt ttgccaatga acaggatacc cgagtgaaag atttcgatcc ctggcgcggc
      661 ttcacctcca gcgctctctc gcccgagtgg cggaagaaac tcagcgagac ggcccgccgt
      721 tgccgcgcgc agattctcaa gatgaccagc ctcgccgcct ccggccatcc cggcggttcc
      781 atgtcgtcgc tcgagctgta cctcactctc taccacatgg cccgcgtcga tccccgcaac
      841 ccgatgcgcg atgaccgcga ccgcatcatc gtgtcgcacg gccacacctc gccgggcgct
      901 tatacggcgc tggcggccgc cggtttcttc gatcccgccg cggccatgca cggtttccgc
      961 caggccggct cccccttcga agggcatgtc gagcggagcg tccccggcat cgaatgggac
     1021 accggcaacc tcgggcaggg gctctcggtc ggcatcggca aagcgctcta cgcgcggctc
     1081 tcgggtctga atttccacac gttcgtgatc atgggcgacg gcgagcagca gaaggggcag
     1141 gtgggcgagg cgcgccgggt cgccgccaaa ttcgccctct cccgtctgac cgcgatcatc
     1201 gaccgcaacc gcctccagat ttccggccgc accgtcgaca tcctgccgca ggatctcgcc
     1261 gccgagtggg aggccgacgg ttggcaggcg gtcgaaatcg acggccactc atttgaccag
     1321 atctacgggg aactgcaccg cgccgtcacc aatgaaggcc gcccgatcgt catcatcgcc
     1381 aacaccgtca tgggcaaaga cgtcggcttc atggaaaacg acgaggcttt ccacggggct
     1441 ccggtcgccc ccgacatgct caccccggcc ctcgccctcc tgggcgacca cgggaaagac
     1501 ctccccgcgc tgcgcgagct gcgcaagcag gggccgccgc ccgcgttcaa gatcccccgg
     1561 cccccctacc cgccggtcga aaccgggcag ccggccacct acggcgccga cgtcaaaatg
     1621 gacaaccgca cggctttcgg caaagctctc ctggccgtcg ccgatctcaa cctcccccgc
     1681 gaggacttcg tcatggccgt cttcgactgt gacctcgccg gctcggtcaa gactgcggcc
     1741 ttcggccaga agtaccccga caattttttc cagtgcggca ttaccgaaca caacactgcc
     1801 gccatcgccg gctccctctc cgccgagcgc gccgtctcga tctgggccga cttcgcggtc
     1861 ttcggcgtcg acgagacttt caaccaggcc cgcctgaacg atatcaacca cgccaatctg
     1921 aaactcttct gcacccacac cggcgttcag gtcggcgagg acggcaagac ccaccagtgc
     1981 atcgactact tcgccctcct caactcgact ttcggctgga gagtcatcac cccggccgat
     2041 cccaaccaga ccgaccgcat cacccggtgg gttttgagcc ggccgggcaa cttcgccgtg
     2101 ttcatggggc gttccgcgac gccgatcatc cccgacgccg acggccgtcc cttcttcggc
     2161 ggcgactacg agtaccgcta cgggcgcatg gacgtcctcc ggcgcggcga gcgcctcgcc
     2221 ctcgtcgccg ccggcaacat gtgcgcaatc gctctcgacg cctggcagcg gctgtcccga
     2281 gagggcgtgg gaatctcgct catcagcgtg gccgactggt cggactttca cgccgatgat
     2341 ctccagatgc tggcggccca cgagcacctt gtcgtgctcg aggaccacaa cgtcaagacc
     2401 ggattgggga ccgctcttgc cgccgagctc ttccaggccg gcgcccgctc cacgctcacc
     2461 aaaatgggcg tgacggcgta cgcctcctcg ggcccgcccg ccgacctctt ccggcttttg
     2521 gggctcgacc cggcgtccgt ggtcgctcgg gtgaaggcca ttctcggctg acggtcctcg
     2581 gcagtatccg cgccgtcggg ccgcaggcgt ccgccggccc gggcggggct gtgcctcccg
     2641 ccgccgggcg t
//
LOCUS       DAOOKS010000241         2650 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_47527_length_2650_cov_2.812331, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000241 DAOOKS010000000
VERSION     DAOOKS010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47527_length_2650_cov_2.812331"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(234..305)
                     /locus_tag="PK112_04745"
     tRNA            complement(234..305)
                     /locus_tag="PK112_04745"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(271..273),aa:Lys,seq:ttt)
     gene            complement(410..1690)
                     /locus_tag="PK112_04750"
     CDS             complement(410..1690)
                     /locus_tag="PK112_04750"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010627322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HPC11390.1"
                     /translation="MAKKNKIVLGCQWGDEGKGKIVDLLAAQADVIARFQGGANAGHT
                     IVVDDRKYILHLIPSGIIHSGKACYIGNGVVLDPFGFIEELDFLASRGIDYAGRLWVS
                     PAANLVLPYHKLIDAVLESSRGTASIGTTMRGIGPAYIDKVARNGIRVIDLFHPDRLR
                     KRLEYQATVKRQYLEGSSDERADLGRTFDALMEIAPILRPLVTDVSLAISLPYRAGRK
                     ILYEGAQGAMLDVDLGTYPFATSSNTTVGGALTGLGIGPHMIDEVIGVVKAYTTRVGA
                     GPFPTELVDNAGDLLRTRGDEYGATTGRPRRCGWLDLVQLRHAVRINGVTSIAITKLD
                     VLDDFDEIPVCTGYRLGDEQFDQVPLDLADLARVKPEYRTLPGWRTRTTGTTVFDHLP
                     AQARNYLNFIARDLGVTIAIVSTGARRDETIAVG"
     gene            complement(1674..2303)
                     /gene="yihA"
                     /locus_tag="PK112_04755"
     CDS             complement(1674..2303)
                     /gene="yihA"
                     /locus_tag="PK112_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HPC11391.1"
                     /translation="MATAPTITTCDFVGSFVSAGQAPADRRPHIAFAGRSNVGKSSLL
                     NRLVGRRKIARVSSDPGKTRALNFYLVNSRFYFVDLPGYGYARVSRQMRAEWGRLLES
                     YLNGDPHLAGLVLLLDARRDPTEQDFQLVAWLAARGIPTLVAVTKTDKLNRDQTNRKI
                     AAVERLFNAPALAFSAVSGLGKNELLGAIFDLVNERAQDIGAPRHGQKE"
BASE COUNT          432 a          791 c          967 g          460 t
ORIGIN      
        1 cttccgcggg ggcgcggcgc cgtcgtgcct cgatcatgga gacaccaacg cggacgggtc
       61 gatcaaggtg tccgacctga cgctcctgat caattacctg ttccggggcg gtccaccgcc
      121 ggcggcttgc tgacgagagg cctgttccgc gcacagtgag atggggccca tggcgaccgc
      181 cgccatgggc cctcgctttt gggcgtcgcg gcagccggag gcgggcaaaa aaatgagggt
      241 ggcagggatt gaacctgcga caccctgctt aaaaggcagg tgctctacca ctgagctaca
      301 cccccaaagg gacacccatc gccggtggcg gcgggtgtgc ggtcaataat agcaggtcgg
      361 cgggccgagg tcaaagagtt tttggcctct ccgcgcccgg gcgcgcggcc tagcccacgg
      421 cgatcgtttc atcgcgccgg gcccccgtgg agacgatggc gatggtcacc ccgagatcgc
      481 gggcgatgaa gttcaggtaa ttgcgggcct gcgccgggag gtggtcgaac accgtcgtcc
      541 ccgtcgtgcg cgtccgccag cccggtaacg tccggtattc cggcttcacc cgggcgaggt
      601 cggcaaggtc gagcgggacc tggtcaaact gctcgtcgcc cagacggtag ccggtgcaca
      661 cggggatctc gtcaaaatca tccaggacat cgagcttggt gatggcgatc gaggtgacgc
      721 cgttgatgcg aacggcgtgg cggagctgga cgaggtcgag ccatccgcag cggcgggggc
      781 ggccggtcgt ggcgccgtat tcatcgccgc gggtgcgaag cagatcgccg gcgttgtcga
      841 ccagctcagt cgggaacggc ccggcgccta cgcgggtcgt gtacgccttg accaccccga
      901 tcacctcgtc gatcatgtgc ggaccgatcc ccagaccggt gagcgcgccc cccaccgtgg
      961 tgttggacga cgtggcgaag gggtaggtcc cgagatcaac gtcgagcatc gccccctggg
     1021 ccccctcgta gaggatcttg cggccggcgc ggtacggcag ggagatcgcc agcgagacat
     1081 cggtcaccag cggccggagg atcggggcga tttccatgag cgcatcaaaa gtgcggccga
     1141 ggtcggcgcg ctcgtcggac gaaccctcga ggtactggcg cttgaccgtc gcctgatact
     1201 cgagccgctt gcggagccgg tccggatgga agaggtcgat gacgcggatg ccgttgcggg
     1261 cgactttgtc gatataggcg gggccgatcc cccgcatggt cgtgccgatc gaggcggtac
     1321 cgcggctgga ttcgagcacg gcgtcgatga gcttgtggta gggcaggaca aggttggccg
     1381 ccggcgagac ccacagccgc ccggcgtagt cgatcccgcg cgaggcgagg aaatcgagct
     1441 cctcgatgaa gccgaacggg tcgaggacga cgccgtttcc gatgtagcag gctttgccgg
     1501 agtggatgat gcccgagggg atgaggtgga ggatgtactt gcggtcgtcg acgacgatcg
     1561 tgtgaccggc gttggcgcct ccctggaagc gggcgatcac gtccgcctgg gcggcgagga
     1621 ggtcgacgat ctttcccttg ccttcatcgc cccactgaca gccgaggacg atcttattct
     1681 ttttggccat gtcgaggtgc ccctatgtct tgcgcacgtt cgttcacgag gtcaaaaatg
     1741 gcgccgagca gttcgttctt gccgaggccg ctcacggcgg aaaacgccag ggccggggcg
     1801 ttgaagaggc gctcgacggc ggcgattttg cggttggtct ggtcgcggtt gagcttgtca
     1861 gtcttggtga ccgcgaccag cgtcgggatc ccgcgtgcgg cgagccaggc gacgagctgg
     1921 aaatcctgct cggtcgggtc gcggcgggcg tccaggagaa gcaccagtcc cgcgagatgc
     1981 gggtcgccgt tcaggtagct ctcgaggagt cggccccact cggcgcgcat ctgccgggag
     2041 acccgggcgt agccatagcc gggcagatcc acgaagtaga agcggctgtt gaccaggtag
     2101 aagttgagcg cccgcgtctt gccggggtcg gaggaaactc tggcgatctt gcggcggccg
     2161 acgaggcggt tcagcagcga cgatttgccg acattggagc ggccggcgaa ggcgatgtgg
     2221 gggcggcggt cggccggcgc ctgcccggcg gagacaaatg aacccacaaa atcgcaggtc
     2281 gttatcgtgg gggcggtcgc catcgcagtc ctcatccgtc gtgtctcttg acaccacatt
     2341 acgcatccat gttgtcgggg gagcagtcgc ggggctgtcc cctgtgagta tagggattcg
     2401 ggaggcgagt gtcaaatagt aaccgggcgg tcgcgctgcc gccgcccggc gccgtcggcc
     2461 gggcggactc aggaggttgc tttaggcgcc gcgcgcgatg cggtaggtcg ctctcacccg
     2521 cgtgcgggtg atcgggttcc gggtcatggc caggcggatg acttcggtga ccgaacgcac
     2581 gtagtggagt ttcagccctt tcaaaaggtc ggcggggagc tcccggatgt ttttgcggtt
     2641 cttctccggg
//
LOCUS       DAOOKS010000242         2643 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_47707_length_2643_cov_2.737249, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000242 DAOOKS010000000
VERSION     DAOOKS010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47707_length_2643_cov_2.737249"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="PK112_04760"
     CDS             complement(<1..601)
                     /locus_tag="PK112_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011033407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HPC11392.1"
                     /translation="MSAAPAGPPESAAPYRRTAEEVLAELGVDARSGLAESEAGARLA
                     RWGRNELAAEGPVPGWRKFFAQFQDPLVILLLIATAVSAALWLSEGDTALPYEAIAIF
                     AVVLLNAVMGYVQQARAENAVAALRRMAAARATVVRGGERRIIPVEELVPGDIIILEE
                     GDTVPADARLIQEAALQTAEAALTGESLPVAKDVLPLAQD"
     gene            complement(598..1617)
                     /gene="galT"
                     /locus_tag="PK112_04765"
     CDS             complement(598..1617)
                     /gene="galT"
                     /locus_tag="PK112_04765"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HPC11393.1"
                     /translation="MSEFRQNKATKEWVVVAPGRGRRPSDFAKETAAPRPLPEYRDDC
                     PFCPGHEDQTPATVLQLPPHGPWRVRVAPNKFAALSPSLPAARTAVGSFLSANGFGIA
                     EVIVESPRHNQTLATMSVAEVTEVLTAYRERQRAASRNPHVNLVTVFRNHGRRAGTSL
                     EHPHSQLIATPIVPPHVRDPLRQAVLHYDSYGTCVYCDMVAEEIRQEERLLDQTPGFV
                     AFCPFAARSPFEARIYPKGHAASFTAIGDDAIAELAAVLRSTLRRLYFLLGNPDYNYI
                     IRSSPVGDEDTRHLHWYIVIIPKISTPAGFEIGSGIYINSVAPESAARALREVDIDGA
                     REEPA"
     gene            2290..>2643
                     /locus_tag="PK112_04770"
     CDS             2290..>2643
                     /locus_tag="PK112_04770"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HPC11394.1"
                     /translation="MKDALVILRECDIQPTPQRIAVVEYVLKCRTHPSAEDVLSYARK
                     KCPTVSRATVYNTLHLLVEKGLLGMQTIREGAVVFDPNVERHHHFVDNDTGEIYDIPW
                     EQLEVRGTERLREFEI"
BASE COUNT          445 a          818 c          943 g          437 t
ORIGIN      
        1 cgtcctgcgc gagagggagg acgtctttgg cgaccgggag gctctcgccg gtaagcgcgg
       61 cctcggccgt ctgcagcgcg gcctcctgga tcagccgggc gtcggcgggc accgtgtccc
      121 cctcctcgag gatgatgatg tcgcccggga cgagttcctc gaccgggatg atgcggcgtt
      181 cgccgccgcg caccacggtc gcccgggccg ccgccatccg gcggagggcg gcgacggcgt
      241 tttccgcgcg cgcctgctgg acgtagccca tgacggcgtt gagcaggacg accgcgaaga
      301 tcgcaatcgc ttcgtacggc agcgcggtat ccccctccga gagccacagg gcggccgaga
      361 cggcggtggc gatgagcagg aggatgacca ggggatcctg gaactgggcg aagaacttcc
      421 gccacccggg cacgggtcct tcggcggcca gttcgttgcg gccccagcgc gccaatcgcg
      481 cgcccgcctc gctctcggcc agtccgctcc gcgcgtccac accgagctcg gccagcacct
      541 cttcggctgt ccggcggtag ggcgcggcgc tctcgggcgg cccggcgggg gcggcgctca
      601 cgccggctcc tcccgcgcgc cgtcgatgtc tacctcgcgc agcgcccggg cggcgctctc
      661 gggggcgacc gaattgatgt agatgccgga gccgatctcg aatcccgccg gcgtggagat
      721 tttcgggata atgacaatgt accagtgcag gtggcgggtg tcctcatcgc cgaccgggga
      781 ggagcggatg atgtaattgt agtcggggtt tccgagcagg aagtagagcc ggcggagggt
      841 ggaccgcagc acagcggcaa gctccgcgat ggcgtcgtcg ccgatggccg tgaagctggc
      901 ggcgtggccc ttcgggtaaa tgcgcgcctc aaagggggag cgggcggcga aggggcagaa
      961 ggcgacaaag ccgggggtct ggtcaaggag gcgttcttcc tgccggatct cttcggcaac
     1021 catgtcgcag tagacgcacg tgccgtagct gtcgtagtgg aggacagcct ggcgaagagg
     1081 atcgcggacg tgggggggga caatgggcgt ggcgatgagc tgggagtggg gatgttccag
     1141 cgaagtgccg gcccgccggc cgtgattgcg gaagacggtc accaggttga cgtggggatt
     1201 gcgcgaggcg gcgcgctggc gctcgcgata cgcggtgagc acttcggtca cctccgccac
     1261 cgacatcgtg gccagcgtct ggttgtggcg ggggctctcg acgatcacct ccgcgatgcc
     1321 gaagccgttg gccgaaagaa acgagcccac cgccgtccga gcggccggca gcgacgggga
     1381 gagcgcggcg aacttgttgg gggcgacccg tacgcgccat ggaccatgcg gcggcaattg
     1441 cagaacggtg gccggagtct gatcctcgtg cccggggcag aacgggcaat catccctata
     1501 ttccggaagc ggccgcggcg cggcggtttc tttggcgaag tcggagggcc gccgtccccg
     1561 tcccggagcg acgaccaccc actctttggt ggctttgttc tgtcggaatt cggacatgtt
     1621 gtgacctcct cacttcggaa ataacgacca aagcgccggg ctccggcaag cttgatcttg
     1681 cggccggcga cgacgagctg ttggctgaga gctgcgactg cgggcgagga cgagcgccag
     1741 agtgcgcggg ggaggcgagc gatcagcggg gcctacctcg gaatcgaggc ccttccgcgc
     1801 cgcctcgccg tcttgctgca cgaccagggt gagtggggat gcgcggagct ggtggccctg
     1861 gcggaggaat gcctccgatc acgttccggc agtcctggat aatcgcacga ggcccggcac
     1921 tcggcggggg atgatgtcgg cggggagcga ggccacagtc gtcggtcatc tgcagtgtaa
     1981 tacgacgcga ctcccggctg cctctcgatg gcaacatttg tcgggaggcg gcgccgaccc
     2041 cggcccggac ttcacactcc catacccggt gctgcctacc tctcgcagtg ttcccacggg
     2101 ccgcgtccaa tcgactgaaa gtcgctggcc gccaatccat tccaacgcgc aagccgggcc
     2161 gggacggcac cccgattttt cggagctcga gtcgcgatct tctcgtccct gggttcggaa
     2221 cgggcatatt tcggctgggc cccgcacttt gttcttgaca ctgtctaaaa cgatacataa
     2281 tctaattcca tgaaggacgc cctcgtcatt ttgcgggagt gcgatatcca acccaccccc
     2341 cagagaattg ccgtggtgga atacgtactg aagtgcagaa cacacccatc ggcggaggat
     2401 gtcctgagct atgcgcggaa gaagtgcccg acggtctcgc gggcgacggt ctacaacacg
     2461 ctccacctgc tggtcgagaa ggggcttctc ggcatgcaga cgatcaggga aggcgcggtt
     2521 gtttttgacc ccaacgtgga gcggcaccat cactttgttg acaacgacac gggggaaatc
     2581 tatgacatcc cgtgggaaca gctcgaggtc agagggacgg agcgactcag ggaattcgag
     2641 atc
//
LOCUS       DAOOKS010000243         2639 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_47792_length_2639_cov_3.748839, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000243 DAOOKS010000000
VERSION     DAOOKS010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47792_length_2639_cov_3.748839"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2157)
                     /locus_tag="PK112_04775"
     CDS             complement(<1..2157)
                     /locus_tag="PK112_04775"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HPC11395.1"
                     /translation="MITRQEALDYHSRPRTGKIEVTPTKPCRTQLDLSLAYTPGVAQP
                     CLEIHENPEDVYKYTAKGNLVAVVSNGTAVLGLGNIGAAAGKPVMEGKGVLFKRFADV
                     DVFDIELNTEDPDEVIKCVQLLEPTFGGINLEDIKGPECFYIEETLKRTMKIPVFHDD
                     QHGTAIISAAALLNALELVKKDIDKIKVVYSGAGAAGIACAKLYCSLGVKHENLLMVD
                     SKGVLYEGRGDKWNKYKGEFVRKTDARTLADAMVDADVFVGVSTKDLVTKEMVKSMAK
                     DPIIFAMANPDPEITYPEAMDARKDVIMATGRSDYPNQVNNVLGFPFIFRGALDVAAT
                     AINEEMKIAATHALATLARQNVPESVAKAYGVDHFSFGREYLIPKPFDSRILIWESSA
                     VAKAAMETGVARNPMDIEEYKKLLSERIGQGRKVMTFISEKARRSPKKIVFPEGHSSR
                     ILRAARIVKDEGIALPIVLGDPADIKKIAAEHEVDLLGIEIIDPATSPRRPAYAQAYW
                     KRRARRGITEDKALWLMRERTYFGMMMLATGDADAVLSGVLSDYPSTVRPALEIIDLA
                     PGATRVSGLFAMLQKDRVYMFADTTVNINPSPEELAEIALLSADVARRFGIEPRIAML
                     SFSNFGSARYPESEKVARATTLLKKMAPDLVVEGELQADAAVMPEMLRKLFPFSKLQE
                     PANVFIFPELNSANIAYKLLQRVGGLDAIGPILMGLS"
     gene            complement(2458..>2639)
                     /locus_tag="PK112_04780"
     CDS             complement(2458..>2639)
                     /locus_tag="PK112_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11396.1"
                     /translation="ANYLDPVSDRLTFITSVEWLPDSQGTGYELFGSTIEESTRRFSI
                     MFGLRMYFSGESDRP"
BASE COUNT          452 a          854 c          802 g          531 t
ORIGIN      
        1 cgacagcccc atgaggatcg gcccgatcgc gtcgagcccg ccgacccgct ggagcagctt
       61 gtaggcgata ttcgccgagt tgagttcggg gaagatgaag acgttggccg gttcctggag
      121 cttggagaag gggaacagtt tgcgcagcat ctccggcatg accgccgcgt ccgcctgcag
      181 ctcgccctcg acgaccaaat ccggcgccat ctttttcaag agagtcgtgg ccctggccac
      241 tttctccgac tcgggatacc gggcggagcc gaaattcgag aacgagagca tggcgatccg
      301 cggttcgatc ccgaaccggc gcgccacgtc ggccgacagc agcgcgatct cggccagctc
      361 ctccggcgag gggttgatgt tcaccgtggt gtcggcgaac atgtacaccc ggtccttctg
      421 gagcatcgca aacagccccg agacccgggt ggcgccggga gcgaggtcga taatttccag
      481 ggccgggcga acggtcgatg ggtaatccga cagcacgccg gagagcaccg cgtcggcgtc
      541 gcccgtcgcc agcatcatca tcccgaaata cgtccgctcg cgcatcagcc agagggcctt
      601 gtcctcggtg atcccgcggc gcgcgcggcg tttccagtag gcctgggcgt aggccggacg
      661 gcgcggcgag gtggccggat cgatgatctc gatccccagc aggtcgacct cgtgctcggc
      721 cgcgatcttc ttgatgtccg ccggatctcc cagcacgatg gggagggcga tgccttcgtc
      781 ttttacgatc cgcgccgctc gaaggatgcg tgacgagtgg ccctccggga acacgatctt
      841 cttcgggctg cggcgcgcct tctcgctgat gaacgtcatg accttccgcc cctgcccgat
      901 ccgctccgaa agcagcttct tgtactcctc gatatccatc gggttgcggg ccacgcccgt
      961 ctccatggcg gccttggcca ccgccgacga ttcccagatc aggatgcgcg aatcaaacgg
     1021 cttggggatg aggtactcgc gcccgaacga gaagtggtcg acgccgtagg ctttggccac
     1081 cgactccggc acgttctgcc gggccagcgt cgccagggcg tgggtggccg caatcttcat
     1141 ctcctcgttg atcgccgtcg ccgccacatc gagcgccccg cggaaaatga aggggaaacc
     1201 cagcacgttg ttgacctggt tcggatagtc cgagcgcccc gtcgccatga tcacgtcctt
     1261 gcgcgcatcc atcgcttcgg ggtaggtgat ttccgggtcc gggttggcca tggcgaagat
     1321 gatcgggtcc ttcgccatcg acttcaccat ctctttcgtc accaggtctt tcgtcgacac
     1381 gccgacaaac acgtcggcgt ccaccatcgc gtcggccagg gtccgcgcat cggtcttgcg
     1441 cacgaactcc cctttgtact tgttccactt gtcgccgcgc ccctcataga ggacgccctt
     1501 ggagtcgacc atgagcaggt tctcatgctt gacgccgagc gagcagtaga gcttggcgca
     1561 ggcaatcccg gccgcgccgg cgcccgaata gacgaccttg atcttgtcga tgtctttctt
     1621 gaccagttcc agcgcgttaa gcagggcggc cgccgagatg atggcggtcc cgtgctggtc
     1681 gtcgtggaac accgggatct tcatcgtccg cttgagcgtc tcctcaatat agaaacactc
     1741 cggccccttg atgtcttcga ggttgatccc cccgaaagtc ggctcgagta gctggacgca
     1801 cttgattact tcgtccgggt cttcagtatt cagctcgatg tcgaatacgt cgacgtcggc
     1861 aaaccgtttg aagaggaccc ctttgccctc catcaccggc ttgccggcgg cggcgccgat
     1921 gttgccaagg ccgagcaccg ccgtgccgtt gctgaccacc gccacgagat tccccttggc
     1981 cgtgtatttg tagacgtcct ccggattctc gtggatttcc aaacagggct gcgccacccc
     2041 cggggtatag gccagggaga ggtcgagctg ggtgcgacac ggcttggtcg gggtgacctc
     2101 gatcttgccg gtgcgcggcc ggctgtggta gtcgagggct tcttgccgag taatcatggc
     2161 gaactgactc ctttgatgcg aaaggggctc ccgcggcgat ttcccgctga ccactgagcc
     2221 ccatgttcag aatttcacat gcgcgaatat acgggctgaa caggccaagt caagcggatt
     2281 tgaccgctcc tgtccggggc ggcctccggg cggtttcgcc gccggcgccg cgggttgccg
     2341 cccgacatcg caagctcggc cgagccgttt ttccgcccct cccgcgtaag tttattgaca
     2401 tatactgaca gccccggtta attggtctga accgaatttg ccaatcacgt ctcccagtca
     2461 gggccggtcg ctctcgcccg agaaatacat cctgaggcca aacatgatgc tgaaacgccg
     2521 ggtcgattcc tcgatagtgg aaccgaagag ctcatatcct gtcccctgac tatccggcag
     2581 ccattccacg ctcgtgatga atgtcagccg gtcgctcacc gggtcaagat agttggcat
//
LOCUS       DAOOKS010000244         2616 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_48364_length_2616_cov_4.233503, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000244 DAOOKS010000000
VERSION     DAOOKS010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48364_length_2616_cov_4.233503"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="PK112_04785"
     CDS             complement(<1..308)
                     /locus_tag="PK112_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11397.1"
                     /translation="MTWISTQFAKHWRNIQYTLVAVLSAALILWPALLDQTVRPVVLN
                     GLYYPFFKLRSAVELLRSQAAANQQLREALMAASLELAASREAVRENARLREQLGYTP
                     P"
     gene            complement(331..783)
                     /locus_tag="PK112_04790"
     CDS             complement(331..783)
                     /locus_tag="PK112_04790"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HPC11398.1"
                     /translation="MKLSKFCDETLVKFNLKSATKDDLIEELVDLVATSNMVKDRDQL
                     LTDVRERENLVTTGVGYGVAFPHAKTKSVKGIVIAFGRSKQGVDFDAMDHKPVHLFFL
                     IAAPEDAIGAHLNVMARLSFLMKSEKNRQKLLEANSPGDVLALMDNVE"
     gene            complement(799..966)
                     /locus_tag="PK112_04795"
     CDS             complement(799..966)
                     /locus_tag="PK112_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HPC11399.1"
                     /translation="MPLSDKLLSKLACPKCRGPLSFDADQNRLLCSACRLSYPVREDI
                     PVLLVDEAEKL"
     gene            complement(987..1946)
                     /locus_tag="PK112_04800"
     CDS             complement(987..1946)
                     /locus_tag="PK112_04800"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HPC11400.1"
                     /translation="MPEGPILDFERPIRELEKKISGMKDFSIGESIELSGEIALLERK
                     LERLREEVYSNLNRWQMVQLSRHPKRPYTLDYIRLMTTGFIELHGDRRFADDPAMVGG
                     LAWLDDQPVMIVGQQKGRDTKEKVRRNFGMAHPEGYRKALRLFRLAEKFDVPIVILID
                     TPGAFPGIGAEERGQAEAIAYNIREMFGLRVPIVIVIIGEGASGGALGIGVGDVVMML
                     QFSWYSVISPEGCAAILWRDAAKAPEAAEALRPTASDLMALKIVDKVIPEPSGGAHSD
                     PEGAAAAVRAEIQGALADLKALPVDRLLERRLAKYRVIGEFDE"
     gene            complement(2090..>2616)
                     /locus_tag="PK112_04805"
     CDS             complement(2090..>2616)
                     /locus_tag="PK112_04805"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPC11401.1"
                     /translation="VGTAVGRMRFPDGSFQATCRRLPQFNNMLFSRGSIFARLMRTNG
                     QYTLPDFPEVTPVPAAAGTMMMIEKRLFRDVGGFDKRFFMFMEDVDLCLRLGLLGYRN
                     YFVPAAGAVHLWGRGSRVGKFRRNLHHHINVWRYFAKHYPNILSYALLPFALALNLLL
                     VTVLPVSQPANRRP"
BASE COUNT          485 a          816 c          858 g          457 t
ORIGIN      
        1 ggtggggtgt agccgagctg ctcgcgcagg cgggcgttct cgcggaccgc ctcgcggctg
       61 gcggccagtt cgagcgacgc ggccatgagc gcctcgcgca gttgctggtt ggccgccgcc
      121 tgcgagcgaa gcagttccac ggcgctgcga agtttgaaaa acgggtagta cagaccgttg
      181 agcaccaccg gccggaccgt ctggtccaag agggcgggcc aaagaatgag cgcggccgac
      241 aaaacggcca cgagagtata ctggatgttg cgccagtgct tggcaaactg cgtcgaaatc
      301 caggtcatac cgggatgatc ggcagggcgg ctattcgacg ttatccatta aggccagtac
      361 atcccccggc gaattcgcct cgagcagttt ctgccggttc ttctcggact tcatcaaaaa
      421 ggacagccgc gccatcacgt tcaggtgagc gccgatggcg tcctcggggg cggcgatcag
      481 gaagaacaaa tggaccggct tgtggtccat ggcgtcgaag tccaccccct gcttgcttcg
      541 cccgaacgcg atcacgatgc ctttgaccga cttggtcttg gcgtggggga aggcgacccc
      601 gtagccgacc ccggtggtga cgaggttctc gcgttcacgg acgtcggtca gcagctggtc
      661 gcggtccttg accatgttgg aggtggcgac gaggtcgacc agttcctcga tgaggtcgtc
      721 cttggtggcc gacttgaggt tgaacttgac cagggtttcg tcgcagaact tagacagttt
      781 cattgcccct acctttgcct acagtttttc cgcctcatcg acgagcagga ccgggatatc
      841 ctcgcggacc gggtacgaga gccggcaggc ggaacagagc agccggttct ggtcggcgtc
      901 aaaagagagc gggccccggc atttggggca ggccagtttg gaaagcagtt tgtcggacaa
      961 cggcatatgc acaactccac gtctacttac tcgtcaaatt ctccaatcac ccggtacttg
     1021 gcgagccggc gctcgagcag ccggtcgacc gggagagctt tgagatcggc gagcgcgccc
     1081 tggatttcag cccggacagc ggcggcggcg ccctcgggat cgctgtgcgc cccaccgctc
     1141 ggctcgggaa tcaccttgtc aacgatcttc aacgccatga ggtcggaggc ggtgggccgg
     1201 agggcctcgg ccgcctcggg cgctttggcc gcgtcgcgcc agaggatggc cgcgcatccc
     1261 tccggcgaaa tcaccgagta ccacgagaac tgaagcatca tcaccacgtc gcccacgccg
     1321 attccgagcg cccccccgga ggcgccttcg ccgatgatga cgatgacgat cggcacccgg
     1381 agcccgaaca tctcgcggat gttgtaggcg atcgcctccg cctgaccgcg ctcttccgcg
     1441 ccgatgccgg ggaaggcgcc gggggtgtcg atgagaatga cgatcgggac gtcgaacttc
     1501 tcggccaggc ggaagaggcg cagggccttg cggtacccct cggggtgggc catgccgaaa
     1561 ttccgccgca ctttttcctt ggtgtcgcgc cccttctgct gcccgacgat catgacgggc
     1621 tggtcatcga gccaggccaa cccgccgacc atggccggat catcggcaaa ccgccggtcg
     1681 ccgtgcagct cgatgaaccc ggtggtcatg aggcggatgt agtcgagggt gtaggggcgc
     1741 ttggggtggc gcgagagctg caccatctgc cagcggttca ggttggagta aacctcctcc
     1801 cgcaggcgct cgagcttgcg ctcgaggagg gcaatctccc ccgacagctc gatgctctcg
     1861 ccgatgctga aatccttcat gccggagatc ttcttctcca gctcacggat cggtctctcg
     1921 aaatccagta tcgggccttc cggcatacgc agtccccgtc gctacttgtg ccgtccgcgg
     1981 cgcgcacagg cttccccggc cgccccgcgg gacgcggtca agtttttaag ataacgatcg
     2041 gcaatgaaat aacaacctca aaaaatggag cgatgggaag ggcggggtgt caggggcggc
     2101 ggttggccgg ctgcgacacc ggcagcaccg tcaccaagag aaggttgagc gccagagcga
     2161 agggcaacag ggcataggac aggatattcg ggtagtgctt ggcgaagtag cgccagacgt
     2221 tgatatggtg gtggaggttg cggcggaact tgccgacccg gctgcctctc ccccacaggt
     2281 gcacggcccc cgcggcgggc acaaagtagt tccggtagcc caggagcccc agccgcagac
     2341 agagatccac gtcttccatg aacatgaaga agcgcttgtc gaacccgccg acgtcccgaa
     2401 acagccgctt ctcgatcatc atcatcgtcc cggccgccgc cggcaccggg gtcacctcgg
     2461 gaaaatccgg gagcgtgtac tggccgttgg tgcgcatgag ccgcgcgaag atcgacccgc
     2521 gcgagaagag catgttgttg aattgcggca ggcggcggca ggtcgcctgg aacgaaccgt
     2581 cgggaaaccg catccgtccc acggccgtgc ccaccc
//
LOCUS       DAOOKS010000245         2613 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_48434_length_2613_cov_3.694683, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000245 DAOOKS010000000
VERSION     DAOOKS010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48434_length_2613_cov_3.694683"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="PK112_04810"
     CDS             <1..526
                     /locus_tag="PK112_04810"
                     /inference="COORDINATES: protein motif:HMM:NF025580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4328 domain-containing protein"
                     /protein_id="HPC11402.1"
                     /translation="LMWIHCAYRVLEVNKVAGLKATAGWAVGWWFIPIANLVKPFEVT
                     KEIFVRSERPPAGGAPWGAGPTPAPAGFVVSTAQSRESSWIVGAWWLTWLAASFATNL
                     SGFLSGAFYGLGGNNQPTAEQYVRGNLLDIGATVLFILAGVLAIMVVRDIDRRQHRAF
                     SQQPAPLPPSAWPR"
     gene            585..1337
                     /locus_tag="PK112_04815"
     CDS             585..1337
                     /locus_tag="PK112_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11403.1"
                     /translation="MPESLIALLVFGGFAVVFVAVIRAARAQRRARIARLQQIGFFEW
                     KDPDQTLVARFVPLISGGGKKAPIGTVLRRTSGAGEMYTFLPHGDTSAAGVLIGRVPD
                     LALPRFRLMRRPPINGRLGTWVVGLVTKLMGGDLQPVAFASRPDLAEQFLAFAETPAA
                     FESFALRHWADLLKGAPVGFGLAGDRDLLAFSFHADLDRSARRRSALPALPSTVTMTR
                     DRQDLCARLAESVRQAEQFIAAAQTSWSAHTV"
     gene            1656..>2613
                     /locus_tag="PK112_04820"
     CDS             1656..>2613
                     /locus_tag="PK112_04820"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HPC11404.1"
                     /translation="MTTRLRRSEIRELLKLTRTPGTISFGGGLPDPVVFPREAVEAAS
                     KRAIRERGDLALQYSPTEGEPFLKEQLAAYMQRHDEIARPEDMLVVSSSQQALDLLGR
                     IFIDPGDPVLIERPTYVGSIQAWRALDADFQGVDMDDDGIIPEKLDAKIRELVDGGRP
                     PKFIYVIPDFQNPSGINLSLERRHEVLAIASRYDLPIIEDSPYRELRFSGELIPSMYS
                     LDTERRVILMKTLSKIYTPGFRLGWIVAPPDVLDKLIMVKQGADLCTSAYVSIITGYL
                     FEDGAIERQVERCRDLYARKADVMLAAMEREMPKIPGLWWSKP"
BASE COUNT          436 a          897 c          804 g          476 t
ORIGIN      
        1 tctcatgtgg atccactgcg cctaccgggt gctcgaagtg aataaggtcg ccggcctgaa
       61 agcgaccgcc gggtgggcgg tcggctggtg gttcatcccg atagccaacc tggtgaagcc
      121 gttcgaggtg accaaagaga tcttcgtgcg gagcgagcgg ccgccggctg gcggagcgcc
      181 ctggggagcc ggtccgacgc cggccccggc cgggttcgtc gtgagcaccg cccagtcgag
      241 agaatcctcg tggatcgtcg gcgcctggtg gctgacctgg ctggcggcct cgttcgccac
      301 caacctctcc gggtttttgt cgggggcgtt ctacggactg ggcggcaaca accagccgac
      361 cgcggagcag tatgtccgcg gcaacctgct ggacatcggc gccaccgtgc tgttcatttt
      421 ggcgggcgta ctcgccatca tggtggtccg cgacatcgac cgccgccagc accgcgcttt
      481 cagccagcag ccggccccgc tgcctccgtc ggcctggccg cgctgacctg tggaaccggg
      541 gccgggcccg tcggatccga actcttatcg gcagtgaggt ctctatgccg gagagcctga
      601 tcgcccttct cgtcttcggc ggctttgccg tcgtctttgt cgccgtcatc cgggcggcgc
      661 gcgcgcagcg gcgcgcccgg atcgcgcgcc tccagcagat cggcttcttc gaatggaagg
      721 accccgacca aaccctcgtg gcgcgttttg tgccgctgat cagcggcggc ggcaagaaag
      781 ccccgatcgg caccgtcctg cgccgcacct ccggcgcggg ggagatgtac actttcctgc
      841 cgcacggcga cacttctgcc gcgggagtgc tcatcggacg ggttcccgac ctggcccttc
      901 cccgcttccg cctgatgcgc cgtccgccga taaacggcag actcggcacc tgggtggtgg
      961 ggctggtcac gaagctgatg gggggagacc tgcagccggt ggcgttcgct tcccggcccg
     1021 atctcgccga gcagttcctt gccttcgcgg agaccccggc cgcgttcgaa tcattcgcgc
     1081 tgcgccactg ggcggacctg ctgaaaggcg ccccggtcgg tttcggcctc gccggcgatc
     1141 gcgacctgct cgccttttcc ttccacgccg acctcgaccg gtcggcccgc cgccgttccg
     1201 cactgccggc gctcccttcg acagtgacaa tgacccggga ccgccaagat ctttgcgccc
     1261 gactggccga gtcggtgcgc caggcggagc aattcatcgc cgccgcgcaa acctcgtggt
     1321 cggcacacac agtgtgacgc cgcggcggcc gaacgcgact tccccacgaa gccggcgaga
     1381 cagcagggct cctcggaaac gcagtcgcac acattacacg cattgaatcc acgcacgagc
     1441 ccagggcggc tctccggaaa atgttaacag actgcctcgt tgcctcttaa catttccgca
     1501 tcgatcgtca ccaaaattgt cctcgtttga caccttcgcc ggtcgatttt cgtaacactc
     1561 ttccgcgaaa ttcgttatct tacggcggaa ctatggcttc tcgttactga cgaatgtagg
     1621 tgaggtccaa tatggcgaaa atgcgatttt ccacgatgac gacgcgactg cgtcgcagcg
     1681 agatccgcga acttctgaaa ctcacgcgga cccccggaac gatctcgttc ggcggcggcc
     1741 tgcccgaccc ggtggtgttt ccccgggagg cggtcgaagc ggcatccaag cgggctatcc
     1801 gggaacgcgg cgatctcgca ctccagtaca gtccgacgga aggggaaccg ttcctcaagg
     1861 agcagctcgc ggcgtacatg cagcgccacg acgaaatcgc ccgccccgag gacatgttgg
     1921 ttgtctcgtc ctcacagcag gcgctcgacc tgctcggccg aatcttcatc gacccgggcg
     1981 acccggtcct tatcgaacgg cccacctatg tgggctcgat ccaggcctgg cgggccctcg
     2041 acgccgactt ccagggagtc gacatggatg acgacggaat catcccggag aaacttgacg
     2101 ccaagatccg ggagctggtc gatggcggcc gcccgcccaa attcatctac gtcatccccg
     2161 acttccagaa cccctccgga atcaacctct cgctggaacg ccgccacgag gtgctcgcga
     2221 ttgcctcgcg ttacgatctg ccgattatcg aagacagccc ctaccgcgaa ctccggttct
     2281 ccggcgagct gatcccgtcg atgtactccc tggataccga gcggcgggtg atcctcatga
     2341 agacgctgtc gaagatttac acgccgggat tccgcctcgg ctggattgtc gctccgcccg
     2401 acgtgctcga caagctcatc atggtcaaac agggcgccga cctgtgcacc tcggcctatg
     2461 tctcgatcat caccggctac ttgttcgaag acggggcgat cgagcgacag gttgagcgct
     2521 gccgcgatct ctacgcccgc aaggccgacg tgatgctggc ggcgatggag cgcgagatgc
     2581 ccaaaatccc cgggctctgg tggtccaaac cga
//
LOCUS       DAOOKS010000246         2608 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_48571_length_2608_cov_2.910302, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000246 DAOOKS010000000
VERSION     DAOOKS010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48571_length_2608_cov_2.910302"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(694..1155)
                     /locus_tag="PK112_04825"
     CDS             complement(694..1155)
                     /locus_tag="PK112_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11405.1"
                     /translation="MRVHLLLSAALLSLAAALVGCGSGKPSDPKTAVIAMFGAMEKND
                     KATLAHLLDLGELMKNLNEDYALQTDEPRHFTSPEQILEDLTDGGKTKERWFAYQRIV
                     NEVEMTADDLATVEVTFVDKEASKGYRTKFGLHVVNDRWKIYTFNAFPTGS"
     gene            complement(1152..2108)
                     /locus_tag="PK112_04830"
     CDS             complement(1152..2108)
                     /locus_tag="PK112_04830"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HPC11406.1"
                     /translation="MTRTLYLAAAGCAVALLLSAAGAAAGEANSRAGTSAFSFLKIDV
                     GARAVAMGGAGTGLADDESALYYNPAGIVGFEQRRFVGGYHSYFADMQSGFVGYIAPI
                     GLTKAVGAYLSYLNYGDFVETDPTGAVLGTFSGGDLLFAVSGAVQPRYGLALGATVKF
                     IYESLHNYTATGVAVDLGGRYSTNRDRLIVGLAVQHLGVQFSGLGTEKDRLPLTVRGG
                     AALRPRGLPLTLAGDLIVPVDNDPSIALGLEYVELKPLYLRIGWNSFGSNYRTGDSDD
                     SRAGLGLGVGFDIKTYHISYAFAPGAELGDSHRFTFSGGIFP"
     gene            complement(2341..>2608)
                     /gene="lptE"
                     /locus_tag="PK112_04835"
     CDS             complement(2341..>2608)
                     /gene="lptE"
                     /locus_tag="PK112_04835"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HPC11407.1"
                     /translation="ADAVLAGTLVSYTRAPYQYTSGDAVQSYRVTMDFDIALVNPKDN
                     SEIWKERMSQIGIYDPATETEEDAQRKAIQNLVQAILNRTTKSW"
BASE COUNT          477 a          789 c          855 g          487 t
ORIGIN      
        1 tgatgaccag taatccaatg ttcttcttca ttgcgtcctt ttctcctccg taacaccgta
       61 acggttggtc aatatacgcg gccggccgaa acggcacaac cccaatcgtg caatctctcc
      121 attttttaga cggccgccac attcgctgcg ttcagcgcaa tcgcacgtgt cgtcaaccgc
      181 cggccggagt ccgcggaggc gcagaaattg gcgcggtggg tggccgtgcc cccaaaaccc
      241 ggtcgtgaat gatggaccgg ccaaatccgg tcgaggtctc acgcagtaaa tattaagtta
      301 ttttattaca gtttattaca gacggctcca tggaggagaa gcggaaaaac cctcaatttt
      361 cacacgctct ccctttgaat tcgtgtggat aagtgtttga caaacaagga ggggtagcct
      421 ataattttga cgagcggggt acgtggaaga tttagaagct tgccatgatg accgttgtcc
      481 ggggatttcc ccctctgtcc ctgagcatgg cgcatggtgg gcttcttttt ttggccggag
      541 tcggcccggt cgccggaggc gaagggaacg gtgcagcccc gcctcgccat cgcacgacct
      601 gacttcgatc ccccggggcc ataaagaagg cacgagtccg aggactcgtg ccgccgtgtc
      661 tttcacgcac gtggcgcgcc ctttcccggc gaactaagat ccggtcggga aggcgttgaa
      721 cgtatatatc ttccagcgat cgttgaccac gtgaaggccg aacttggttc ggtatccctt
      781 cgaagcctcc ttgtcgacaa acgtgacctc gacggtagcg agatcgtccg cggtcatctc
      841 cacctcgttg acaatccgct ggtatgcgaa ccagcgttcc ttggtcttgc cgccgtcggt
      901 gaggtcctcg aggatttgct ccgggctggt gaagtgacgc ggctcatcgg tctgcagggc
      961 gtaatcttcg ttgagattct tcatcagctc gcccaagtcg agcaggtggg cgagggtcgc
     1021 cttgtcgttt ttctccatgg ctccgaacat ggcgatgacg gcggttttgg ggtcggacgg
     1081 tttgcccgag ccgcagccga ccagggcggc ggcgaggctc agcagggcgg ccgagagaag
     1141 caggtgcact ctcatggaaa aatccccccg gaaaaggtga agcggtgact gtcgcccagc
     1201 tccgccccgg gagcgaaggc gtaggatatg tgataggttt tgatgtcgaa accgacgccg
     1261 aggccgagtc cggccctgga atcatcagag tcgcccgtcc ggtagttcga accgaaactg
     1321 ttccagccga tccgcaggta gagcggctta agctccacgt attccagacc gagggcgatc
     1381 gagggatcgt tgtcgacggg gacaatgagg tcgcccgcga gcgtaagggg aaggccgcgg
     1441 gggcggaggg ccgcaccgcc gcggaccgtc agcggcagcc ggtccttctc ggtccccagg
     1501 ccggagaact ggaccccgag gtgctggacg gccaggccga caatcagccg gtcccggttg
     1561 gtgctgtagc ggccgcccag gtcaacggcc acgccggtgg ccgtgtaatt gtgcagactc
     1621 tcgtagatga acttaacggt ggcgccgagc gcgagaccgt accgcggctg caccgcgccg
     1681 ctcacggcga acagaaggtc gccgccgctg aaggtgccga ggacggcgcc ggtcgggtcg
     1741 gtttcgacga agtcgccgta gttgaggtac gacaggtagg cgccgaccgc cttggtcagg
     1801 ccgatcgggg cgatgtagcc gacgaacccg gactgcatgt cggcgaagta gctgtggtag
     1861 cccccgacaa aacggcgctg ttcgaagccg acgatgccgg ccggattgta gtagagggcc
     1921 gattcatcat cggccagccc ggtgccggcg ccgcccatgg cgaccgcccg ggcgcccacg
     1981 tcgattttca gaaacgagaa ggcgctcgtg ccggctcgcg aattggcctc accggcagcc
     2041 gcgccggcgg ccgacagaag cagcgcgacc gcacaacctg cggccgcgag gtacagggtt
     2101 cgcgtcattg gtgccatcct tccagagcga tgatacggtt ccgcgccgcg gctgtcaaca
     2161 gcaatagggc gcgcccggcc aaaggccggc ggacgcccgg ggcgatgcgg cgccggcggg
     2221 ccccgggagg gtcccccggg agcagcccgg cgcaaacggg aggagcgggc ctacgcccgg
     2281 tccggttctt atactcccca gccgcggaaa gaatccggcg ctccgccgcc gatcacgccc
     2341 tcaccagctc ttcgtggtgc ggttgaggat tgcctggacg aggttctgaa tcgccttccg
     2401 ctgtgcatcc tcctccgtct cggtggcggg atcgtagatg ccgatctggc tcatgcgctc
     2461 tttccagatc tcggaattgt ccttcggatt gacgagggcg atgtcgaaat ccatggtcac
     2521 gcggtacgac tgcacggcgt cgccggaggt gtactgatag ggggcgcgcg tgtagctcac
     2581 cagcgtgccg gcgagcacgg cgtcggcg
//
LOCUS       DAOOKS010000247         2606 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_48612_length_2606_cov_3.991768, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000247 DAOOKS010000000
VERSION     DAOOKS010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48612_length_2606_cov_3.991768"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="PK112_04840"
     CDS             complement(<1..270)
                     /locus_tag="PK112_04840"
                     /inference="COORDINATES: protein motif:HMM:NF024231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HPC11408.1"
                     /translation="MAHSALYLALAVLLPIGFHALGVAGRIFLPMHFPILLAGFLAGP
                     AAGVVVGLLAPGLSHLLTGMPPTYAVPLMSLELPMYGLAAGIAYRR"
     gene            complement(308..913)
                     /locus_tag="PK112_04845"
     CDS             complement(308..913)
                     /locus_tag="PK112_04845"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPC11409.1"
                     /translation="MSHDKHQEFFDRLAAEWDLMFTAVDLEFLQHIVDQLDIRQGWDI
                     LDLGCGTGVLFDMLRRRVGETGSVTGVDFSFQMAQRANLNFPFPNVAVVDADAQMLPF
                     RDSAFDMAVAFSAFPHFSDQQKALLETHRVLKPRARFYIIHLESSRDLNQLHQRIGGA
                     VASDLIPPGERLREMFSTSKFVEVTIEDHPGLYLATAMNLK"
     gene            1108..>2606
                     /locus_tag="PK112_04850"
     CDS             1108..>2606
                     /locus_tag="PK112_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HPC11410.1"
                     /translation="MATSVKPDNRTRINNKARNKRNQEYLHVKGAREHNLQNIDVKIP
                     RNTLTVITGLSGSGKSSLAFDTIYAEGQRRYVESLSAYARQFLGLMEKPDVDFIEGLS
                     PAISIEQRSAAKNPRSTVGTVTEIYDYLRLLFARVGVPHCVSCGQPISQQTVEQIVDS
                     VLGFEEGTRLMVLAPLVRGKKGEHKDVIDEARGEGFVRLRINGEIVESEADISLDKNK
                     KHTIEAVVDRLVVKESSRTRLADSVETALKVGQGTVLINIKGQDLLYSEQFACLNCNI
                     SYEEPTPRLFSFNSPFGACPVCDGLGQKMEIDPRLVIPDPSLSISDGAIRPWGGHDLA
                     NWYRYMLKGLARHFDFKLSTPFQKLPEKVRKVVLYGSGREEIEFSYEHMTGRGHGGGI
                     YRAPFEGVIPHLERRYRQTESNNVRMWIENFMSVSDCPACRGARLKPEALAVILDRET
                     IDSITSMSIKQAAAFFRRITLTKRQETIARQILKEIRERLQFLCDVGLD"
BASE COUNT          543 a          883 c          764 g          416 t
ORIGIN      
        1 ccggcggtag gcaatccccg ccgccagacc gtacatgggc agttccagcg acatgagcgg
       61 caccgcgtag gtcggcggca tgccggtgag caggtgcgac aaccccggcg ccagcagccc
      121 caccaccacg ccggccgccg gcccggccag aaacccggcc agcagaatgg gaaagtgcat
      181 cggcaggaaa atccgcccgg ccacccccag cgcgtggaac ccgatcggca aaagcaccgc
      241 cagcgcaagg tagagcgccg agtgcgccac gaatcgggtc ctggcggtag ttggtgacac
      301 cgatagacta cttcagattc atcgccgtcg cgaggtacag gcccggatgg tcctcgatgg
      361 tcacctcgac aaacttgctc gtgctgaaca tctcccgcaa ccgctccccc ggcggaatca
      421 ggtcggacgc caccgccccg ccgatccgct ggtggagctg gttcaaatcc cgggaggact
      481 ccaggtgaat aatgtagaaa cgggctctcg gcttgagcac ccgatgggtc tccaggagcg
      541 ctttctgctg gtcggagaaa tgcggaaagg ccgaaaacgc gacggccatg tcgaaggcac
      601 tgtcccgaaa cggcagcatc tgcgcgtcgg catcaaccac cgcgacattg ggaaagggga
      661 aattaaggtt cgcccgctga gccatctgaa acgaaaaatc cacccctgtg acagatccgg
      721 tctcgcccac ccgacgccgc agcatgtcga acaatactcc ggtgccgcaa ccgagatcca
      781 ggatgtccca gccctgccta atatcaagct ggtcaacaat atgctggagg aactcaagat
      841 ccaccgcggt gaacatgagg tcccattccg ccgccagccg gtcgaagaat tcctgatgct
      901 tatcgtgcga catgacccga atttagcaca ccgcccgcgc ccggtcaagg agctttcctc
      961 ccctcgctcc ggcgtccttg cgcttggccg ggccaggcgg gctctggtag cggcggcccg
     1021 cataatgtga tggcgaaatc gaagtcgccg ttggcggcgg ggaaccggtg tagtatatta
     1081 cgggttgaca caaaatcgga gatgccgatg gcgaccagcg tgaaacccga caaccgcacc
     1141 cggatcaaca acaaggcccg taacaagcgc aaccaggaat acctgcatgt caaaggggcg
     1201 cgcgaacata acctccagaa tatcgacgtc aaaatccccc gcaacaccct gaccgtcatt
     1261 accgggctct ccggatccgg caaatcctcc ctcgccttcg acaccatcta cgccgagggg
     1321 cagcggcgct atgtcgagtc cctctccgcc tacgcccgcc agttcctcgg cctgatggag
     1381 aagccggacg tcgatttcat cgagggactc tccccggcca tttcgatcga acagcgcagc
     1441 gcggccaaga acccccgctc caccgtcggc accgtcaccg aaatctacga ctacctccgt
     1501 ctgctcttcg cgcgcgtcgg cgttccccac tgcgtctcct gcggccagcc gatttcgcag
     1561 cagacggtcg agcaaatcgt cgacagcgtc ctcggtttcg aggaggggac gcgcctgatg
     1621 gtgctcgcgc cgctggtccg cggcaagaag ggggagcaca aggacgtgat cgacgaggcc
     1681 cggggcgagg gttttgtccg cctgcgcatc aacggcgaga tcgtcgaatc cgaggccgac
     1741 atcagcctgg acaaaaacaa gaagcatacg atcgaggcgg tggtcgaccg cctggtcgtg
     1801 aaagaaagct cgcgcacccg gttggccgat tcggtggaga cggccctcaa ggtggggcag
     1861 ggaaccgtcc tcatcaacat caagggacag gacctcctct attccgagca gttcgcctgc
     1921 ctcaactgca acatcagcta cgaggagccc accccccggc tgttctcgtt caattccccg
     1981 ttcggcgcct gcccggtgtg cgacggtctg gggcagaaga tggagatcga cccgcggttg
     2041 gtgatccccg acccttcgct ttcgatttcc gacggcgcga tccgcccctg gggcgggcac
     2101 gatctcgcca actggtaccg ctacatgctc aaggggctcg cccgccactt cgatttcaaa
     2161 ctctccaccc cgttccagaa gctgcccgag aaagtccgga aagtggtgct ctacggctcc
     2221 gggcgcgagg aaatcgaatt ctcgtacgag cacatgacgg gccgcgggca cggcggcggc
     2281 atctaccggg cgcccttcga aggcgtcatt ccgcacctcg aacgccgcta ccgccagacc
     2341 gaatcgaaca acgtccggat gtggatcgag aatttcatgt cggtgagcga ctgcccggcc
     2401 tgccgcggcg cccgcctcaa gccggaggcg ctcgccgtca tcctcgaccg cgagacgatc
     2461 gacagcatca cgtccatgtc gatcaagcag gcggccgcct tcttccgccg catcaccctg
     2521 accaagcgcc aggagacgat cgcccgccag atcctcaaag agatccgcga gcggctccag
     2581 ttcctgtgcg acgtgggcct cgatta
//
LOCUS       DAOOKS010000248         2603 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_48697_length_2603_cov_3.629121, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000248 DAOOKS010000000
VERSION     DAOOKS010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48697_length_2603_cov_3.629121"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..963
                     /locus_tag="PK112_04855"
     CDS             <1..963
                     /locus_tag="PK112_04855"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPC11411.1"
                     /translation="PSARFPFFVSTIVPACNEAGNIEQFCRLFADMLQSAPFDGELVY
                     IDDGSTDDTLDKIKAAAAAYPFVRYVSHQHRRGVTDALRSGFSAARGDVFVFYPADLQ
                     FLPEDIPALVAPIADGADLVAGWKQGEYQKRFVSTVYNWLSRKIFNLKVHDLNACKAF
                     RRGVVEQIFMRKDWHRYLVVLAANEGFRVEEVKVRLYPRAWGRSKYQSIWRVPVGVLD
                     MLAVKFQLTFLRKPLMFFGFTGLGFFLLGFLVGLWAVYLKYWRGETQLPLLYLVLLLV
                     GLGIALFAMGFITEGQAAIKEELASMRREVESRLRQLSRDRDHG"
     gene            1180..>2603
                     /locus_tag="PK112_04860"
     CDS             1180..>2603
                     /locus_tag="PK112_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11412.1"
                     /translation="MAKKKKAGDAPPAAKRETSRLNIEQSPWFTPVAFAIIFIGLVIL
                     FRDFIFSRLMLAGSDMIQAGIFFRSFLVDYYHAHGAVPRWNPYIFGGMPFVDAFHGDI
                     FYPLSFLKYFGNIFRMLGWVQFWHIYLAGIFMYLAARQFRLSKIAALLAGVAYMFAPY
                     LVSFVAPGHDGKMFVTALFPLTVYFLDRGIARGSFFDFSMLGLVIGFIVLSPHPQMSY
                     FTLWALAAYMVFRLVFLFREKRRIRLLVRPAALTAYAVVIGLLLSAIQFYPGYTYTRE
                     FSPRADTKRGWEWATSWSLHEEEAVSLVIPEFAGVMTRGAQTFYWGKNAFKDNSEAVG
                     AVTFFLALLGAFFARRREAYFFAGLALFALLYALADTTPVFRLFYWLIPMVKSLRAPS
                     MIMFLFSFSAAMLAGMAVQRLRDARQAGNADPPGARFEWLLWGVPGGLFVLALLFSAA
                     GEGMLNLWTSLFYSEAAQTQVARGV"
BASE COUNT          375 a          834 c          787 g          607 t
ORIGIN      
        1 ccgtcagctc gctttccgtt cttcgtctcc acgattgttc cggcctgtaa cgaggcgggc
       61 aacatcgagc agttctgccg gctgtttgcc gacatgcttc agtcggcccc tttcgacggt
      121 gagttggtct acatcgacga cggttcgacc gatgacacgc tggacaagat caaggcggcc
      181 gccgcggctt acccttttgt ccgctatgtt tcgcaccagc accggcgcgg cgtgaccgac
      241 gcgctccgct ccgggttcag cgccgcgcgg ggagatgtgt ttgttttcta tccggccgat
      301 ttgcagtttc tccccgaaga catcccggcc ctggtggcgc cgattgccga cggcgccgac
      361 ctggtggccg gctggaaaca gggcgagtac cagaagaggt ttgtctcgac agtctataac
      421 tggctgtccc gcaagatctt caacctcaag gtccacgatc ttaacgcctg caaagcgttc
      481 cgccgcggag tggtcgaaca gatcttcatg cgcaaagact ggcaccgcta tctcgtcgtg
      541 ctcgccgcca acgagggttt ccgggtcgag gaagtcaagg tccgtttgta cccgcgggcc
      601 tggggcagat cgaagtacca gtcgatctgg cgcgtcccgg tcggcgtgct ggacatgctg
      661 gcggtgaagt tccagttgac ctttctgcgc aagccgctca tgtttttcgg cttcaccggc
      721 ctggggttct tcctgctcgg gttcctcgtc ggtctgtggg ccgtgtacct gaaatactgg
      781 cgcggggaaa cgcagctccc gcttctgtac ctcgtgctgt tgctggtcgg tctggggatc
      841 gctctctttg ccatgggatt catcaccgag ggtcaggccg ccattaaaga ggagcttgcc
      901 tccatgcgcc gcgaggtcga gagccggctg cgtcagctca gccgggaccg cgaccacggc
      961 tgagccgttt tcctgccgtc atctttccgg gggccatgat tctcggggcc gcgccacgcc
     1021 atgatccggg gggacaaaaa aggttgcttt gccgatggtt tgtggttatg cttggcctcg
     1081 ggtatcggcg cggccgaaac gccctaacag tttgtctcac aagcccgtgg ccgtgtccga
     1141 cacttcccgg aagctgaggc gcagagagcg gagaatcgtt tggccaaaaa gaaaaaagcg
     1201 ggggatgctc cccccgccgc aaagcgcgaa acgagtcgcc tgaatatcga gcagtcgccc
     1261 tggttcaccc cggtcgcctt cgccatcatc ttcatcgggc tcgtcatcct cttccgcgac
     1321 ttcatcttct cccgactcat gctcgccgga tcggacatga tccaggccgg gattttcttc
     1381 cgctcctttc tggttgacta ctaccacgcc cacggcgcag tgccgcgctg gaacccctac
     1441 atcttcggtg ggatgccttt tgtcgatgcc tttcacggcg acattttcta ccccctgtcg
     1501 ttcctgaaat acttcggcaa catcttccgc atgctcggct gggtgcagtt ctggcacatt
     1561 tatctcgcgg gcattttcat gtaccttgcc gcccggcagt ttcggctctc gaaaatcgcc
     1621 gccctgctgg ccggcgtggc gtacatgttc gctccttacc tcgtgtcgtt cgtcgcgccc
     1681 ggccacgacg gcaagatgtt tgtgaccgcc ctgtttccgc tgacggtgta tttcctcgac
     1741 cgggggatcg cccgcgggtc ctttttcgac ttctcgatgc tcgggctcgt gatcgggttc
     1801 atcgtactct cgccgcaccc gcagatgtcg tatttcacac tctgggcgct ggcagcgtac
     1861 atggtttttc gacttgtatt tctctttcgg gagaagcgca ggatccgcct gctggtgcgt
     1921 ccggcggcgc tcaccgccta cgccgtggtg atcggcctcc tcctgtcggc catccagttc
     1981 tatcccggtt acacttacac ccgggagttt tcgccgcgcg ccgacaccaa gcgcggctgg
     2041 gagtgggcga cgtcgtggtc cctccatgag gaggaggccg tctcgctggt gatcccggaa
     2101 ttcgccgggg tgatgacccg gggggcgcag acgttctact ggggcaagaa cgctttcaag
     2161 gacaattccg aggcggtcgg ggcggtgaca ttcttcctgg cgctgctggg cgcctttttc
     2221 gcccgccgcc gcgaggccta cttcttcgcc ggcctggctc tcttcgccct cctctatgct
     2281 ctggccgaca ccaccccggt tttccgatta ttctactggc tcatcccgat ggtgaaatcg
     2341 ctccgcgccc cgtccatgat catgttcctg ttttcgttct cggcggcgat gctggccggg
     2401 atggcggtcc agcgtctgcg cgatgcccgt caggccggga atgccgaccc gcccggcgcg
     2461 cgtttcgagt ggctcctctg gggcgtaccc ggcgggctgt tcgtcctcgc gctgctgttc
     2521 agcgcggcgg gggaggggat gctgaatctc tggacctcgc tgttctacag cgaggcggct
     2581 cagacccagg tggcccgggg cgt
//
LOCUS       DAOOKS010000249         2598 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_48846_length_2598_cov_3.145891, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000249 DAOOKS010000000
VERSION     DAOOKS010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48846_length_2598_cov_3.145891"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="PK112_04865"
     CDS             complement(<1..683)
                     /locus_tag="PK112_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HPC11413.1"
                     /translation="MVEDVIHDHCGVCGVFGHSRAAELTYLGLYALQHRGQESAGIVT
                     SDNGSIHYHKGMGLVSEVFANPQVLAGLTGGRAIGHTRYSTTGASSLINIQPFLITNR
                     SRHLAIAHNGNLTNSTELRTKLDARGSIFQTTSDTEIILHLAARSRKRTRLEQVCDAL
                     ARVKGAYSLLFLTEDTLIAARDPFGFRPLALGKYRKTWVVASETCAFDLIGARYVRDI
                     EPGEVLEISA"
     gene            953..1738
                     /locus_tag="PK112_04870"
     CDS             953..1738
                     /locus_tag="PK112_04870"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HPC11414.1"
                     /translation="MNSDRPQDRPPVDKFGLIHRLAVDAAGGEEPQEVYRRGLRLSAD
                     LVGLAAAALVVWNRDHVLTLNVSHARSEDYRRRLDALERELFDRLRQTQQLASAYMSF
                     EGDPPVHAFTLPLRYAGRTYGAVIGLQEGPRTIVSEEALLETLAAVLALTWVAGSQNR
                     EEADLRSALTKERKSAVIETAVTVNHEVNNPLTAILGNVQLLLLKRDDLDDELKAKLR
                     TIEQSALKIKDVTQKLMRLTSPRSIDYSDGTKMIDLNDGGEEA"
     gene            complement(1773..2546)
                     /locus_tag="PK112_04875"
     CDS             complement(1773..2546)
                     /locus_tag="PK112_04875"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPC11415.1"
                     /translation="MRVLLSPVWAVTAAVLLASAAAGTDLWKAAEVTYTTSQGLEMHG
                     WLHLPAAASQPGRPLIALLPMMGTAHGAYDLFLDSLAARLRRDTTAATPTVLALDLRG
                     HGASTRLHGKEISYKEMEPGDWAPAPGEVAEFIRRAARDHPEAIDSLNITLVGASIGA
                     NAAAIAAPMVPGVRGIALLSPGSDYRGLAPAEALKAFEGRVLICAAREDRYAAESAGR
                     LAKVREGPVLRIFEGGEHGTNLIDRTPEAMSGLLDWLEE"
BASE COUNT          443 a          892 c          844 g          419 t
ORIGIN      
        1 gccgagatct cgagcacctc gccgggctcg atgtcccgca cataccgggc gccgatcaga
       61 tcgaacgcgc aggtctccga cgccaccacc caggtcttgc ggtacttgcc gagcgccagc
      121 ggccggaaac caaaggggtc gcgggcggcg atgagcgtgt cttcggtgag aaacagcagc
      181 gaataggccc ccttgacccg cgccagggcg tcgcaaacct gctccaggcg cgtcctcttg
      241 cgggaccgcg ccgccaggtg gaggatgatt tcagtgtccg aggtcgtctg aaaaatcgag
      301 ccgcgggcgt cgagttttgt gcgcagctcg gtggagttgg tcaggttgcc gttgtgggcg
      361 atcgccagat ggcggctgcg gttggtgatc aggaatggct gaatgttgat cagcgaggag
      421 gccccggtgg tcgagtaacg ggtgtggccg atcgcccggc cgcccgtcag gccggccagg
      481 acctgggggt tggcgaagac ctcggacacc agtcccatcc ccttgtggta gtggatcgag
      541 ccgttgtcgg aggtgacaat gccggccgat tcctggcccc ggtgctggag cgcgtagagc
      601 cccaggtagg tgagttcggc ggcgcgggag tgaccgaata ccccgcagac gccgcagtgg
      661 tcatgtatca cgtcctctac catgccgtta gctcctcatg ggcgccttgg aaaacaccca
      721 gtatacgccc ggctcggata aatgtcaacg attcgagcgc cgcattttgc atatgtctgt
      781 gaacacaaat gcaccgatcc gccggcggat cggttctccc gcacggccgg ccggctgcga
      841 aaactcatgg cacataggcc caaaactcct tattttcaag ggtacctcag agttactcca
      901 gcgcgcgggc gcccggcgcc gcggggcacc gcgtttgctc aaatgagaca cgatgaacag
      961 tgaccggcca caggaccgcc cgcccgtcga taaattcggc ctcattcacc gcctggcggt
     1021 cgacgccgcc gggggcgagg agccccagga ggtctaccgg cgggggctgc gcctgagcgc
     1081 cgacctggtc gggctggccg ccgccgccct ggtggtgtgg aaccgtgacc acgtcctcac
     1141 cctcaatgtc agccacgccc gcagcgaaga ctatcgccgc cggctcgatg ccctcgagcg
     1201 ggagttgttc gaccgcctcc gccagaccca gcaactggcc tcggcgtaca tgtcgttcga
     1261 gggcgacccg cccgtgcacg ccttcaccct ccccctccgc tacgccggga gaacctacgg
     1321 cgcggtcata ggactgcagg aggggccgcg gacaatcgtg tccgaggagg ctctgctgga
     1381 aaccctcgcg gccgttctcg cgctgacctg ggtggccggc tcgcagaacc gggaagaggc
     1441 cgatctgcgc tctgcactca ccaaggaacg caagagcgcc gtcatcgaaa cggcggtgac
     1501 ggtcaaccac gaggtcaaca acccgctgac cgccatcctc ggcaacgtcc aactgctgct
     1561 gctcaagcgc gatgatctcg acgatgaact caaggcgaaa ctgcggacca tcgagcagtc
     1621 cgccctcaag atcaaagacg tcacacagaa actgatgcgc ctgacctctc cccggtcgat
     1681 cgattattcc gacggcacca agatgatcga cctcaacgac gggggagagg aggcctagac
     1741 cccggccgcc cgcgtcgact tccgaaagtg cgtcactcct ccagccagtc gagcagcccg
     1801 ctcatcgcct cgggagtgcg gtcgatcagg tttgttccgt gttcgccgcc ttcgaagatg
     1861 cgcagcaccg gtccctcccg cactttcgcc agtcgcccgg cggactcggc ggcgtagcga
     1921 tcctcgcggg cggcgcagat gagcacgcgg ccctcaaatg ccttcagtgc ctccgccggt
     1981 gcgagccccc ggtagtcgct gcccggcgac agcagcgcga ttccccggac ccctgggacc
     2041 atcggcgcgg cgatcgcggc ggcattggcg ccgatcgacg cgccgaccag cgtgatgttc
     2101 agggagtcga tggcctccgg gtggtcccgc gccgcgcggc ggataaactc ggccacctcg
     2161 cctggggcgg gcgcccagtc ccccggctcc atctctttgt acgaaatttc tttgccgtgc
     2221 agtctggtcg aggccccgtg gccgcgcagg tcgagcgcga gcacggtcgg cgtcgcggcc
     2281 gtcgtgtctc gccgtagccg cgccgccagc gaatccagga acaggtcgta tgcgccgtga
     2341 gcagtgccca tcatcggcag cagcgcgatc agcggccggc cgggttggga cgccgccgcc
     2401 ggcaggtgga gccagccgtg catttccagc ccctgcgagg tcgtgtacgt gacctcggcg
     2461 gctttccaca agtcggtgcc ggccgccgcc gatgcaagca gtacggccgc cgtcaccgcc
     2521 cagaccgggg aaaggagaac cctcatgtcg cctcctgatt ccatgcgcca cctcctcggc
     2581 cggcgccgac ggtccgtc
//
LOCUS       DAOOKS010000250         2586 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_49177_length_2586_cov_2.857369, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000250 DAOOKS010000000
VERSION     DAOOKS010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49177_length_2586_cov_2.857369"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1738)
                     /locus_tag="PK112_04880"
     CDS             complement(<1..1738)
                     /locus_tag="PK112_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015993313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11416.1"
                     /translation="MTKPVLVNNFDPIGQFKSGISSGTPWDSIVDFATSKSFCGKRLY
                     PRQMTLLKLIYLETESMTDYDMEVINSWAEGFKNVNNPMGVQPDIWDRIEYLKANGFT
                     HFPHVQMVMGRRASKGIIGSILATERIAWLYSLGSWQQHFNQVPGQVAEITVVANSLT
                     QAVTRQFRDIRNAVFACEYLRPHIVGDKQTEFYIRTPGDEQTIIENNLAGITSDREIA
                     TIYCKASSSVSTSGRGGTGLFNAYDEFAHMLAGTGSTKTGEEIYDAFQPSLDQFGHHA
                     MTYCASSPFSKIGKFYDLYQQGRITMDSYNLREGKLKTTSFIEEAQSQDIDLDPDEIS
                     AAVAEPTFLVVQLPSWDTYRDWERSREIPVLPGRTRTFPRWNSPVQYEPKEDGTPDER
                     VQYRRMRRNPDKFKVERGGQFATVQDAYLNEVMVDRMFLTPDWRPPLEIQHQGKFSIA
                     YRAHADPSRTNANFGFCIGHLEQAPADEHGISWPHVIIDVLHVWKPEDFKDHTIDYVQ
                     VGEELDDYLRRFPSITKMTYDQFNSAGLIAHQKRAFPNIRVLEKTFTLKENQDRCERF
                     KSALNLGWVHCLA"
     gene            complement(1764..>2586)
                     /locus_tag="PK112_04885"
     CDS             complement(1764..>2586)
                     /locus_tag="PK112_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11417.1"
                     /translation="TGKYIPGIINYETKVVMGFIPRPKRPDQRPMLFSVCGTGVPWWV
                     GPDADTARAVEDKYRWQPIGYPATAVPMGKSIQAGKDELENQFHIHREQVVAYGAALL
                     GYSQGAIVVSELFEEQIRPIGGRLRWAYLHLTKACTWGNPSREKGKVWPDAGGPPASP
                     HTRGVVENRMIDTPDWWRNYAHKGDLYTDCPDDESGENRTAIWKLIRNGDAFRGPDSL
                     LRQVLELTGTVRDAHQISETTGMVKAMMDALIFFGKQTRPHVNYSTSEAIAYLRS"
BASE COUNT          451 a          728 c          870 g          537 t
ORIGIN      
        1 cggccaggca atgcacccag ccgaggttga gggcgctctt gaaacgctcg cagcgatcct
       61 ggttttcttt gagagtgaaa gttttctcca gcacccggat attggggaag gcccgcttct
      121 ggtgggcgat caggcccgcc gagttgaact ggtcgtaggt catcttggtg atcgagggga
      181 accgccgcag gtagtcgtcg agttcctcgc ccacctggac gtagtcgatc gtgtggtctt
      241 tgaagtcctc gggcttccag acgtgcagga cgtcgatgat gacgtgcggc caggagatgc
      301 cgtgctcgtc ggcaggtgcc tgctccaggt gaccgatgca gaaaccgaaa ttggcgttgg
      361 tgcgcgacgg gtcggcgtgg gcgcgatagg cgatcgagaa cttcccttga tgctgaatct
      421 ccagcggggg acgccagtcg ggggtcagga acatccggtc gaccatcacc tcgttgaggt
      481 aggcgtcctg gacggtggcg aactggccgc cacgttcgac cttgaacttg tcgggattgc
      541 ggcgcatccg gcggtactgc acccgctcgt cgggggtgcc gtcctctttc ggctcgtact
      601 gcaccgggga gttccagcgg gggaaggttc gggtgcggcc gggaagaacc ggaatctcgc
      661 gcgaccgctc ccagtcgcga taggtgtccc aactgggtag ctggacgacc aggaaggtcg
      721 gctcggcgac ggcggcgctg atctcgtcgg ggtcgaggtc gatgtcctgg gactgggcct
      781 cctcgatgaa gctggtggtc ttcagcttcc cctcgcgcag gttgtaggag tccatagtga
      841 tccggccctg ctggtacagg tcataaaact tgccgatttt cgagaagggc gaagaggcgc
      901 agtaggtcat ggcgtgatga ccgaactggt cgagcgaggg ctggaaggcg tcgtagattt
      961 cctcgccggt cttggtggag ccggtaccgg ccaacatgtg ggcaaactcg tcgtaggcgt
     1021 tgaaaagccc tgtgccgcca cgaccgctgg tggagaccga actggaggcc ttgcagtaga
     1081 tggtggcgat ctcccggtcg gaggtgattc cggccaggtt gttctcgata atggtctgct
     1141 catcgcccgg ggtgcggata taaaattctg tttgtttatc gccgactatg tgtggcctca
     1201 gatactcgca ggcgaacacg gcgttgcgaa tatcccggaa ctgccgggtc acggcctggg
     1261 tcaggctgtt ggcgacgacg gtgatctcgg cgacctgacc gggaacctgg ttgaagtgct
     1321 gctgccagga gccgaggctg tagagccagg cgatccgttc ggtggccaga atactgccga
     1381 taatgccctt cgaggcgcgg cgtcccatca ccatctggac gtgcggaaaa tgagtgaagc
     1441 cgttggcctt cagatactcg atccgatccc agatgtcggg ctgtaccccc atcgggttgt
     1501 tgacgttttt gaaaccctcg gcccaggagt tgatgacctc catgtcgtag tcggtcatcg
     1561 actcggtttc gaggtagatc agcttgagca gggtcatctg ccggggatag agcctcttac
     1621 cgcagaatga tttgctggtg gcgaaatcaa caatcgaatc ccagggcgtg ccggaggaga
     1681 ttccggactt gaactggccg atgggatcga agttgttaac caatacgggc ttggtcatga
     1741 cacccagcat ataacgcggc gcgtcatgac ctcaggtacg caatcgcctc ggaggtcgaa
     1801 tagttcacat gagggcgagt ttgtttgccg aagaagatca gggcatccat catcgccttc
     1861 accatcccgg tcgtctcact gatctggtgg gcgtcgcgga cggtgccggt caactccagc
     1921 acctggcgca gcaggctgtc ggggcctcgg aaggcgtctc cgttgcggat cagcttccag
     1981 atcgcggtcc ggttctcgcc cgactcatcg tctggacagt cggtgtacag gtcgcccttg
     2041 tgggcgtagt tgcgccacca gtcgggggtg tcgatcatac ggttctcgac aacacctcgg
     2101 gtgtgcggcg aggcgggcgg cccaccggcg tcgggccaga ccttgccctt ctcccggctc
     2161 gggttgcccc aggtgcaggc cttggtgagg tgcaggtagg cccaccgcag ccgaccaccg
     2221 atcggacgaa tctgctcctc gaacaactcg gagacgacaa tagcgccctg actgtagccc
     2281 aggagcgcgg caccgtaggc gacgacctgc tcgcgatgga tgtggaactg gttctccagt
     2341 tcatccttcc cggcctggat ggatttgccc atcgggacgg cggtggccgg gtagccgatc
     2401 ggctgccaac ggtacttgtc ctcgaccgcg cgggcggtgt cggcgtcggg gccgacccac
     2461 cacgggacac cggtgccgca gacgctgaac agcatcggtc tctggtcggg gcgctttggt
     2521 cgtgggatga agcccatcac gaccttggtc tcgtagttga tgattcccgg aatgtatttg
     2581 ccggtc
//
LOCUS       DAOOKS010000251         2585 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_49204_length_2585_cov_2.815810, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000251 DAOOKS010000000
VERSION     DAOOKS010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49204_length_2585_cov_2.815810"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1229
                     /locus_tag="PK112_04890"
     CDS             <1..1229
                     /locus_tag="PK112_04890"
                     /inference="COORDINATES: protein motif:HMM:NF014544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HYR domain-containing protein"
                     /protein_id="HPC11418.1"
                     /translation="LVTLRVTDPQGGVATCEATVTVVDVTPPIITCPSDRTFECDDIG
                     DFGWPTATDNCDSDPAITLASRDSVPGGCPQEYRLVLGYKAEDFSGNAAGCSQTITII
                     DTTSPVVDCYDTLGFVFTSPTEAEVFWDASATDNCSPAPTVTCDPPSGTTFPIGEHTV
                     TCIADDGCANTDTCAFTFRLVPFDLHPTSCPNPIAVKSVVPAAILGTSDFDVLDVDPS
                     SLRARGVAPLRWTYEDVSTPVGDDPNQNCECTRAKGDGRKDLTLKFDHAALVATFAPG
                     ELVDGGSVMLQITGLLKDGTPLAGGDCLILRYKPKDTPAESGPVAVTELGAWNSPNPF
                     NPATVIGYQLPEPGHVKITVFNMLGQVVAHLIDQWQEAGQHSVSWSGERAASGVYFYR
                     LEVGEAQLTRKMLLLK"
     gene            1462..>2585
                     /gene="lnt"
                     /locus_tag="PK112_04895"
     CDS             1462..>2585
                     /gene="lnt"
                     /locus_tag="PK112_04895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HPC11419.1"
                     /translation="MALATRIWRVILPDDVNVRRRRLELTVWAFVLSLAFYPGPWGFL
                     AWVALVRPIMIFARLGGKAAFRSAYLFGFLFNLSSLYWVALVTPPGMITAVAIVALYY
                     AVVLCLFVRLHRWKAAAGYVALPLVWVGMEYFRTLSEFAFPWSDLGYTQAHYLWVLQI
                     VSVIGVHGLSLLIVAVNVLLAQVFRPSLSYERRVGALFGAAATVGLVTAYGWVVLPPH
                     PKPGTFGVALLQGSVPLDVKWSPDSVDENFALYERLTASVADSAVKLFVWPETAAPCY
                     ITHAPECQAAVGRIAAESGTPHLVGTLAARMVGGKMRHYNSAVQFDGQGVLTAQYDKV
                     KLVPFSEHVPYQDHLRFLEKDFLRKYLTFIDEGRVQWWSD"
BASE COUNT          393 a          816 c          900 g          476 t
ORIGIN      
        1 ccctggtcac gctccgggtc actgaccccc agggcggcgt ggcgacctgt gaggcgacgg
       61 tgacggtggt ggatgtgacg ccgccgatta tcacctgccc ctccgaccgg acgttcgagt
      121 gcgacgatat cggcgacttc gggtggccga cagcgaccga caattgcgat tcggatccgg
      181 ccatcaccct ggcgtcgcgc gattccgtgc ccgggggctg cccgcaggag taccggctgg
      241 tgctcggata caaggccgag gatttctcgg gcaacgcggc cgggtgcagc cagacgatta
      301 cgatcatcga cacgacctcg ccggtagtgg attgttacga cacgctgggg tttgttttca
      361 cgtcgccgac ggaagcggag gtgttctggg atgcgagcgc tacggataac tgcagcccgg
      421 cgccgaccgt gacgtgcgac ccgccatctg gcacgacttt cccgatcggc gagcacacgg
      481 tcacctgcat cgcggatgac gggtgcgcga acaccgacac ctgcgcgttc acgttccggc
      541 tggtgccgtt cgatctccac ccgacctcat gccccaaccc gatcgcggtg aaatcggtgg
      601 tgccggcagc gatcctcggc acgtcggatt tcgatgttct cgacgttgat ccgtcatcgc
      661 tgcgggcaag gggggtggcg ccgctgcgct ggacgtatga ggatgtgtcg acgccggtgg
      721 gcgacgaccc caaccagaac tgcgaatgca cgcgggcgaa gggggacgga cggaaggatc
      781 tgacgctcaa gttcgaccac gccgcgctcg tggcgacttt tgcgcccggc gagctggtgg
      841 acggcggcag cgtcatgctc cagatcaccg gtctcctgaa ggacggaacg ccgctggccg
      901 gcggcgactg cctgatcctc cggtacaaac cgaaagacac gcccgcggag tccggcccgg
      961 tggcggtgac cgagctgggg gcgtggaaca gcccgaaccc gtttaacccg gcgaccgtga
     1021 tcgggtacca attgccggaa ccggggcatg tgaagatcac cgtcttcaac atgctcggcc
     1081 aggtcgtggc gcacctgatc gaccagtggc aggaggcggg acagcactcg gtctcctggt
     1141 ccggcgagcg cgccgcctca ggtgtctact tctaccgcct ggaagtcggg gaggcccaat
     1201 tgaccaggaa gatgctcctg ctgaagtagc cgtttcgttt cacgccgcag gataccgcag
     1261 cccccggccg ggcgatcgcc ggccgggggt ctttcgcggt ccgatcgggc gcgctcgcac
     1321 cggccgactc ctcttaaagg cttgtgccgg gaggaccggc agggtacttt ctcccgtact
     1381 tcggcccgcc ggcggaggga agccgccggt caccgcgacc cgaccggtga acgggcggtc
     1441 cgaacaaacc ggagatcggc aatggcactg gccacgcgca tatggcgggt gatcctgccc
     1501 gacgacgtca acgttcgccg gcggcggctg gaactgacgg tgtgggcgtt tgtcctctcg
     1561 ctggcgttct accccgggcc gtgggggttt ctcgcctggg tggcgctggt gcggccgatc
     1621 atgattttcg cccgactggg aggaaaagcg gccttccgct cggcctacct gttcgggttc
     1681 ctgttcaatc tctcctcgct gtactgggtg gcgctggtga cgccgccggg gatgatcacg
     1741 gcagtggcca tcgtcgccct ctactacgcc gtcgtgctgt gcctgtttgt tcggctgcac
     1801 cgctggaaag cggcggccgg gtatgtcgcg ctgccgctgg tttgggtggg gatggaatat
     1861 ttccggacgc tgtcggagtt cgcctttccc tggtcggacc tcgggtacac gcaggcgcac
     1921 tatctctggg ttctgcagat cgtgtcggtg atcggggtgc acgggctgtc gctcttgatc
     1981 gtggccgtga atgtgctcct cgcgcaggtg ttccggccgt cgctctccta cgagcgccgg
     2041 gtcggggcgc tcttcggcgc ggcggcgacc gtgggtctgg tcacggccta cgggtgggtc
     2101 gtgctgccgc cccatccgaa gccggggacg ttcggggtgg cgctcctgca ggggtcggtg
     2161 ccgctcgatg tcaagtggtc gccggactcc gtggatgaga acttcgccct ctacgagcgg
     2221 ctgacggcga gcgtcgccga cagcgcggtc aagctgtttg tctggcccga gaccgcggcg
     2281 ccctgctata tcacgcacgc cccggagtgc caggcggccg tggggcggat cgccgcagag
     2341 tcggggaccc cccatctggt gggaacgctc gcggcgcgca tggtgggggg aaaaatgcgg
     2401 cactacaact cggccgtgca gtttgacggc cagggcgtcc tcactgcgca gtacgacaaa
     2461 gtcaaactcg tgccgttctc cgaacacgta ccctaccagg accacctgcg gtttctggag
     2521 aaggatttcc tgcgcaagta cctcacgttc atcgacgagg gaagagtgca gtggtggtcc
     2581 gattt
//
LOCUS       DAOOKS010000252         2583 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_49254_length_2583_cov_3.361946, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000252 DAOOKS010000000
VERSION     DAOOKS010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49254_length_2583_cov_3.361946"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="PK112_04900"
     CDS             <1..269
                     /locus_tag="PK112_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11420.1"
                     /translation="RVPPRRPAQPKFLAGDLSTRFLEEEFPENNYLVLDDKLREQIAI
                     AAALETAARERTIIVQNNARGGAAPSNWVLVHRRSVLKPFSGSR"
     gene            269..781
                     /locus_tag="PK112_04905"
     CDS             269..781
                     /locus_tag="PK112_04905"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11421.1"
                     /translation="MRRYLITVDGREFDISLDPSMQSGRLLCNGRPREIITHLLGPSR
                     ALVLIDNESLEVDVRANAYDTKKTLFVHGMEIPVTIEDYHLAQLRKTAGIRSGAEIDR
                     VLRAAMPGLVLEVKVRPGEIVRRSQPLLIIEAMKMENIIKAQGEGTVKTVFVAPGKSV
                     EKNDKLLEFA"
     gene            781..2355
                     /locus_tag="PK112_04910"
     CDS             781..2355
                     /locus_tag="PK112_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HPC11422.1"
                     /translation="MSDDTKLTTSGIPIPVTATAADAPAAPGPRHAGEYPFTRGVYPT
                     MYRGRLWTMRQYAGFGTAAETNRRFKYLLQRGQTGLSVAFDLATQIGYDSDHPMAAGE
                     VGRTGVAIDSLADMEKLFDGIPLDKVSTSMTINAPAVILLAMYIAVGRKQGVPAAQLS
                     GTVQNDVLKEFIARGTYILPPKPSMKIITDIFAYAAKHLPKYNTISISGYHIREAGST
                     AAQEVAFTLANAVAYVEAALAAGLSIDDFAPRLSFFFAAHNDLFEEVAKFRAARQIWA
                     ELVRDRFRSRDEKSMLLRFHTQTGGSTLTAQQPENNIVRTAIQALAGVLGGTQSLHTN
                     SYDEALGLPTEKSVEIALRTQQIIAHESGAAATVDPLGGSYYIEYLTAAMDKRIRALL
                     AEIESRGGAVACIENGYFRDEIAAAAYDFQRRVETRRQIVVGVNEFTSHSPGIPAVLR
                     VDPELERNQVRELAKLKATRDRKAVAESLAAVRDAARQNKNVVEPIIDAVAHYATVGE
                     ISDIFREVWGEYHENL"
     gene            2342..>2583
                     /locus_tag="PK112_04915"
     CDS             2342..>2583
                     /locus_tag="PK112_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11423.1"
                     /translation="MKTFKLRRLAFAGALLALVGCGGQSDTDTADSAAAAGADSLTLD
                     KQLEVQMNAVFDRLRYDDKSGLWENEFEYLREERTF"
BASE COUNT          476 a          971 c          735 g          401 t
ORIGIN      
        1 atcgagttcc accgcgccgt cctgcgcaac cgaaatttct cgccggcgac ctctccacgc
       61 gcttcctcga ggaggaattt ccggagaaca actacctcgt cctcgatgac aagctccgcg
      121 agcagatcgc gatcgcggcc gctctcgaga cagccgcccg cgagcgcacg attattgtcc
      181 agaacaacgc gcgcggcggc gcggccccct ccaactgggt gctcgtccac cgccgctccg
      241 tcctcaaacc gttcagcggg agccgctgat gcgccgctat ctcattactg tcgacggccg
      301 ggaattcgac atttcccttg accccagcat gcagtcgggg cgcctgctgt gcaacggccg
      361 tccccgcgaa atcatcaccc acctcctcgg tcccagccgg gcgctcgtcc tcatcgacaa
      421 cgaatcgctc gaggtcgatg tccgggcgaa cgcctacgac accaagaaga cgctcttcgt
      481 ccacggcatg gaaatcccgg tcaccattga ggactaccac ctcgcgcagt tgcgcaagac
      541 cgccggcatc cgctccggcg ccgagatcga ccgcgttctg cgcgccgcca tgcccggcct
      601 ggtgctggaa gtcaaagtcc ggccggggga gatcgtccgc aggagccagc ccctgctcat
      661 catcgaggcc atgaaaatgg aaaatatcat caaggcgcag ggggagggaa ccgtcaagac
      721 cgtcttcgtc gcccccggca agtcggtcga gaaaaacgac aaactcctgg agttcgcgtg
      781 atgtctgacg atacgaaact gaccacctcg gggattccca ttccggtcac ggcgacggcc
      841 gccgacgcgc ccgctgcccc cggtccccgg cacgccggcg agtacccgtt cacccgcggc
      901 gtctacccga ccatgtaccg cggccgcctc tggaccatgc gccagtacgc cggcttcggc
      961 accgccgccg aaaccaaccg ccgtttcaag tacctcctcc agcgcgggca gaccggcctc
     1021 tcggtcgcct tcgacctcgc cacccagatc ggctacgatt ccgaccaccc catggccgcc
     1081 ggcgaggtgg gccgcacggg agtcgccatt gactcgctcg ccgacatgga gaagctcttc
     1141 gacggcatcc cgctggacaa ggtctccacc tccatgacca tcaacgcccc ggccgtcatc
     1201 ctgctcgcca tgtacatcgc cgtcggccgc aagcagggcg tgcccgcggc gcagctctcc
     1261 ggcactgttc agaacgacgt gctcaaggaa ttcatcgccc gcggcaccta catcctgccg
     1321 ccgaaaccct cgatgaagat catcaccgac atcttcgcct acgcggcgaa gcacctgccg
     1381 aagtacaaca cgatctcgat ttccgggtac cacatccggg aggcgggctc gaccgcggcg
     1441 caggaggttg ccttcaccct ggccaacgcc gtcgcctatg tcgaggcggc gctcgccgcc
     1501 ggcttgtcga tcgacgactt cgcgccgcgc ctgtcgtttt tcttcgccgc ccacaacgac
     1561 ctcttcgagg aggtggcgaa attccgcgcc gcccggcaga tctgggccga gctcgtccgc
     1621 gaccgcttcc gttcccggga tgaaaagtcg atgctcctgc ggttccacac gcagaccggc
     1681 ggttccaccc tcaccgccca acagcccgag aacaacatcg tgcgcacagc catccaggcc
     1741 ctcgccggcg ttctcggcgg cacccagtcg ctgcacacga actcgtacga tgaggctctc
     1801 ggcctgccga ccgagaagtc ggtggagatc gccctgcgca cccagcagat catcgcccac
     1861 gaatccgggg ccgccgccac cgtcgacccg ctcggcggct cttactacat cgagtacctg
     1921 acggccgcga tggacaaacg catccgggcg ctcctggccg agatcgagtc gcgcggcggc
     1981 gcagtcgcct gcattgagaa cgggtatttc cgcgacgaaa tcgccgccgc cgcctacgac
     2041 ttccagcgcc gcgtcgagac ccgccgccag atcgtcgtcg gcgtcaacga gttcacctcg
     2101 cacagccccg gtatcccggc cgtcctcagg gtcgatcccg aactggaacg caaccaggtc
     2161 cgggaactgg ccaaactcaa agcgacccgc gaccgcaaag ccgtcgccga atcgctggcc
     2221 gccgtgcgcg acgccgcccg gcagaacaag aacgtggtcg aaccgatcat cgacgccgtc
     2281 gcccactacg ccactgtcgg cgaaatctcc gacatcttcc gcgaggtatg gggtgagtac
     2341 catgaaaacc tttaagctcc gccgcctggc gttcgccggc gcgctcctgg ccctcgtcgg
     2401 ctgcggcggc caaagcgaca ccgacacggc cgactcggcc gcggccgccg gggccgattc
     2461 cctgaccctg gataagcagc tcgaggtcca gatgaacgcc gtcttcgacc gcctgcgcta
     2521 cgacgacaag tccggcctct gggaaaacga gttcgaatac ctgcgtgagg agcgcacctt
     2581 cga
//
LOCUS       DAOOKS010000253         2581 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_49311_length_2581_cov_2.816706, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000253 DAOOKS010000000
VERSION     DAOOKS010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49311_length_2581_cov_2.816706"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="PK112_04920"
     CDS             <1..459
                     /locus_tag="PK112_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HPC11424.1"
                     /translation="RLNNPALSVGLPPFLTKGAGMFSGLMLSQYTADSLIVEQRILSM
                     PASVQSIPAAADQEDFVSMGMNTAIKNDQILENAYGVLGIEFMAAAQALDFRDYKFGV
                     GTRTVHDIVRRHVAFLDIDRPLYPDHTAMREVVRSCEILDGVEAAVGSLG"
     gene            complement(554..979)
                     /locus_tag="PK112_04925"
     CDS             complement(554..979)
                     /locus_tag="PK112_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11425.1"
                     /translation="MRTLTKEDVMFTRRFAALTLGAALVSLAAAPGVMSKDEGASAAP
                     AAAPAAAEGKTWYLVISPHTQEGCLAALDSVAAEGAAVLQQFDWGCMAGDHTGYARVQ
                     AAGKAEALKMVPASHRSKARAIELNKFTLDQIRAFHESH"
     gene            1436..2236
                     /locus_tag="PK112_04930"
     CDS             1436..2236
                     /locus_tag="PK112_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11426.1"
                     /translation="MANRCAWVFGAALAAAVTAAGCGGGGTLAIVADRSDGLAADGAA
                     RDWDALSARYIEADRVVVGAATDGERLFVLLRFRDPQYAAQIRSEGLTLALDPTGGFG
                     ERFVLHYRGMPAGTDGVPAALQAELRRLRSAAPGDTLRAEELRIAIRDEIETKPIPAT
                     GEEGPSAAAGFDQGFFVYEFQVPLGKSAVRQYRVPAAPDAEVGVVLEWGGRAEADLLN
                     EIAGGGLMVDGPLFPTADEPPRRDGAEDPGKSRLGRHEIRLRVSLGGR"
     gene            complement(2233..>2581)
                     /locus_tag="PK112_04935"
     CDS             complement(2233..>2581)
                     /locus_tag="PK112_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11427.1"
                     /translation="TYSGQCDLLPTLMGLLGGDYTHESWGRDILRVPPADSGFAIFNS
                     SYRIGYLVPDFFLLEHLGGKPGLFDPDFLGQEEAMVNEQYPDVLRRLQRRLHYYLQAA
                     EQATLPEAQRRGL"
BASE COUNT          392 a          811 c          943 g          435 t
ORIGIN      
        1 cgtctgaaca acccggccct gtcggtcgga ctgcccccct tcctcaccaa aggggcgggg
       61 atgttctccg ggctgatgct ctcccagtac acggccgaca gcctgattgt cgagcagcga
      121 atactctcga tgccggcgtc ggtgcagtcg atcccggccg ccgccgatca ggaggatttc
      181 gtctcgatgg ggatgaacac cgcgatcaag aacgaccaga tcctggagaa cgcctacggg
      241 gtgctgggga tcgagttcat ggcggcggcc caggcgctcg atttccgcga ctacaagttc
      301 ggcgtcggca cccggaccgt gcatgacatc gtacgccgcc acgtcgcgtt cctcgacatc
      361 gaccggccgc tctacccgga ccacacggcg atgcgggagg tggtccggtc gtgcgagatt
      421 ctggacggtg tggaggccgc ggtcggaagt ctcggatagc ggcgccgccg ggacgggctt
      481 atgcctcacc gggcgcggca ggctcaggcc gggcctgccg cgcgcggggg tgaggccctt
      541 gcggcaaccg ccgctagtgg ctctcgtgga aagcgcggat ttggtcgagg gtgaacttgt
      601 tcagctcaat cgcccgcgcc ttggaccggt gtgaagccgg caccattttc agcgcctccg
      661 ctttgccggc cgcctgcacc cgggcgtaac cggtgtggtc gcccgccatg cacccccagt
      721 cgaattgctg cagcaccgca gcgccttcgg cggccacgct gtcgagcgcc gcgagacaac
      781 cctcctgggt gtgcggagaa atcacgagat accacgtttt cccctcggca gcggccggcg
      841 ccgccgccgg ggcggccgat gccccctcgt ctttgctcat gacgcccggg gccgccgcca
      901 atgacaccag cgcggcgccg agggtgagtg ctgcaaacct ccgggtgaac atgacgtcct
      961 ccttggttag ggttctcata cgaatgacaa ccgatgtcgg gcagttttgc cgatgaatcc
     1021 gcctggcgca tcgccatcct gtcttgcgcc catactcgct cccgggccgg cctggacagc
     1081 ggcccatatc tccgcatgca aaagacactg acggtgtgag tgcgccggac gcaggacggt
     1141 tgaccgtgcc ggcgatactg gatagaatta gtctctgttg gcggattttg tcaagcgata
     1201 ttttgggggg ctcccccggc ggggtggccc ggtgcgggcg ccgccggagt cggcccgccg
     1261 actgccgtcc gttcggaggg cgcccctcgc tgtgccgcaa gaggttgcgg tggctccggc
     1321 gcaccctccc cgtaggtctc cgtcagcagc ggttaaacat tgcggaggtc tgcggccaat
     1381 gtcaaattga gggggtgaac cagtgaatga cgtggattgg gagacggagc agactatggc
     1441 gaaccgatgc gcatgggtgt tcggcgcggc gttggcggcg gcggtgacgg ctgccgggtg
     1501 cgggggaggc gggaccctgg ctatcgtggc cgaccgcagc gacggcttgg cggccgatgg
     1561 cgcggcgcgg gactgggatg cgctcagcgc gcggtacatc gaagccgacc gcgtcgtcgt
     1621 gggggcggcc accgacggcg aacgcctgtt cgtgctgctg cggttccgcg acccccagta
     1681 cgcggcccag atcaggagcg aggggctgac gctcgcgctg gatccgacgg gggggttcgg
     1741 cgagcggttc gtgcttcact atcggggaat gccggcgggc accgacggcg tgccggcggc
     1801 gttgcaggcc gagcttcgcc gcctgcggtc agcggcgccc ggcgacacgc tccgggcgga
     1861 agaacttcgg attgccatcc gcgatgaaat tgagaccaaa ccgattccgg ccaccgggga
     1921 ggagggacca tcggccgccg ccggtttcga ccagggtttc ttcgtctacg agttccaggt
     1981 gccgctgggg aagtcggccg ttcggcagta ccgggtgccc gcggcgccgg atgcggaagt
     2041 cggggtggtg ctggagtggg gcgggcgggc tgaggcggat ctgctgaacg aaattgcggg
     2101 cggcgggctc atggtggacg gtccgctgtt tccgaccgcc gacgagccgc cgcgccgcga
     2161 cggggcggag gatccgggca aatcgcgtct cggccggcat gagattcgat tgcgggtcag
     2221 cctgggcggg cgctaaagcc cgcgccgctg cgcctccggg agggtcgcct gctcggcggc
     2281 ctgcaggtag tagtgcagcc gcctctgcag acgccggaga acatcggggt actgctcgtt
     2341 caccatggcc tcttcctgtc cgagaaagtc ggggtcgaac aggccgggct tgccgccgag
     2401 gtgttccagc agaaagaagt cggggacgag ataccctatc cgatacgagc tgttgaatat
     2461 ggcgaagccg gagtcggcgg gggggactcg gaggatgtcc cgtccccagc tctcgtgcgt
     2521 gtagtcccct ccgaggagtc ccatgagggt ggggagcagg tcgcactgcc ccgagtaggt
     2581 g
//
LOCUS       DAOOKS010000254         2581 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_49317_length_2581_cov_2.548694, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000254 DAOOKS010000000
VERSION     DAOOKS010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49317_length_2581_cov_2.548694"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2223
                     /locus_tag="PK112_04940"
     CDS             <1..2223
                     /locus_tag="PK112_04940"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPC11428.1"
                     /translation="GFLLAASAAGPAFSEVMSETSDHVTFALGDGLAYVTGTVSDSAA
                     QVATVSLGIGFDSLFEQLNGAITEIHVKVLYDTTRMSFLAAEADPGWYAYFDAQESPH
                     GVVQMQMTLDTTSPLHTASAGTREYGRLRFIHECWPQDSLPGGVNFSRNGMDNSVTVN
                     GIIYSPGSEGWDDGSVDRMPDSAYHITLEIADTAVTGEVGEEFVLTEYATTDFRLSLV
                     WQAYTFDTAKLEFRGFEMPPYFGGTVTLRSDTIVITALNFSGVDEGHHFPLVHLTLAP
                     RCPLGNTSTVLNAVPGWLTVWVSECSGSIDPAAVSDPSVTIAMADTAAYRARYLGEPV
                     TMQDDGQLLKFGVLMRSTFAAGMGSQSDTGLTMNIDWTPNLDFAGIDHYAPAVKFLDL
                     SQGDLTAVYQTWDAARANYLPGGGDFDSLVTLKVRFDTAGFTPSWNNRYLRPKFIHDF
                     GIGHGRTVVPDTVGCLRADSVNGILDAWSGGAFDSVEVLMGQLLAGGGSSSTACGEVD
                     FYVRGTCAIDSFSVRITVPTWACIYGIIDRMTGVIPTFVNYHTYDIQTNSLFAGVGPT
                     GDPVKVCRLRIGLKSPCLRGKPYGIGISVTNGKVWDYAAGGPMYAATNGSTITLKCSS
                     TGTGCSSCGSGNVDDDIPDDPNAKVEPTAPSTFALHQNFPNPFNPATTIQFDLPVAAE
                     WRIEIFNTLGQLIREFHGASAPGSVTVEWDASAYASGVYLYRATAADFVETRKMLLLK
                     "
     gene            complement(2307..>2581)
                     /locus_tag="PK112_04945"
     CDS             complement(2307..>2581)
                     /locus_tag="PK112_04945"
                     /inference="COORDINATES: protein motif:HMM:NF015060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HPC11429.1"
                     /translation="VWRRDGFAGARAALVLFLAQLALNGVWTYLFFGRQWVGLALVDI
                     LALLAAVIITMVLFRKHDRRAGYLLAPYIVWLAYASTLNLGVLVLN"
BASE COUNT          456 a          865 c          824 g          436 t
ORIGIN      
        1 ggcttcctgc tggccgcgtc ggcggccggg ccggcgttct ccgaggtcat gtccgaaacg
       61 tcggaccatg tgacctttgc cctcggcgac ggcctcgcct acgtgacggg tacggtgagc
      121 gactcggcgg cccaggtcgc aacggtatcg ttgggcatcg gttttgactc cctcttcgag
      181 cagctcaacg gcgccatcac cgagatccac gtaaaagtgc tgtacgacac cacgcggatg
      241 agctttctgg cagcggaggc cgaccccggc tggtacgcct acttcgatgc tcaggaaagc
      301 ccgcatggag tcgtgcagat gcagatgacg ctggacacaa cgagcccgct gcatacggcc
      361 tcggccggca cgcgcgagta tggccgcctg aggttcatcc acgagtgctg gccccaggat
      421 tcgctgcccg gcggcgtgaa cttctctcgc aacgggatgg acaactcggt gactgtcaac
      481 ggcattatct actcgcccgg aagcgagggg tgggacgacg gctcggtcga ccggatgccc
      541 gactcggcat accatatcac gctggaaatc gccgataccg cggtgacggg tgaggtcggc
      601 gaggagtttg tactcaccga gtacgcgacc accgactttc gcctcagcct cgtctggcag
      661 gcgtacacgt tcgacaccgc caagctggaa ttccgggggt ttgagatgcc gccctacttc
      721 ggcggcaccg tcacgctgcg gagcgatacg atcgtcatca ccgcgctgaa tttcagcggc
      781 gtggatgagg gccaccactt cccgctcgtt cacctcacgc tggcgccgag gtgtccgctc
      841 ggcaacacct cgacggtgct gaacgccgta cccggctggc tcacggtgtg ggtcagcgag
      901 tgctccggtt cgatcgatcc ggcggctgtg agcgacccgt cggtcacgat tgcgatggcc
      961 gacacggccg cctaccgggc gcgctacctg ggcgagccgg tgacgatgca ggacgacggg
     1021 cagctgctga aattcggcgt gctcatgcgc agcaccttcg ccgcgggcat ggggtcgcaa
     1081 tccgacaccg gcctgaccat gaacatcgat tggacaccca acctcgactt cgcggggatc
     1141 gaccactatg cgcccgcggt gaagttcctc gatctgagcc agggcgacct gacggcggtc
     1201 taccagacct gggacgccgc gcgcgccaac tacctgccgg gcggcggcga cttcgacagt
     1261 ctggtcacgc tcaaggtgcg gttcgacacg gccggattca cgcccagctg gaacaaccgc
     1321 tacctccgtc cgaaatttat ccacgatttc ggcatcggcc acggccgaac ggtcgtcccc
     1381 gacacggtcg ggtgcctccg ggcggattcc gtcaacggca tcctggacgc ctggtcgggc
     1441 ggggcgtttg actcggtgga ggttctcatg ggccaactcc tcgccggcgg cggcagttct
     1501 tccacggcct gcggcgaggt ggacttctac gttcgcggga cctgcgccat cgattccttc
     1561 tccgtgcgca tcacggtgcc cacctgggcg tgcatctacg ggatcatcga ccggatgacc
     1621 ggggtcattc ccactttcgt caactaccac acttacgaca tccagaccaa ttccctgttc
     1681 gcgggcgtcg ggccgacggg cgatcccgtg aaagtctgcc ggctccgcat cgggctgaaa
     1741 tctccctgcc ttcgcggaaa accctacggc atcgggatct cggtcacgaa cggcaaggtg
     1801 tgggactacg cggccggcgg tccgatgtac gccgccacga acggctccac catcactctg
     1861 aagtgcagct ccaccggaac cggctgctcg tcgtgcggaa gcgggaatgt ggatgacgac
     1921 attccggatg accccaacgc gaaggtcgag cccacggccc cctcgacctt cgccctgcac
     1981 cagaacttcc cgaacccgtt caacccggcg acgaccattc agttcgatct gcccgtcgcc
     2041 gccgagtggc ggatcgagat cttcaacacg ctgggccagc tcatcaggga attccacggc
     2101 gcctcggcgc ccggatcggt gacggtcgag tgggacgcct cggcgtatgc gtcaggcgtc
     2161 tacctctacc gcgccacggc ggccgatttc gtggagacca ggaagatgct gcttctgaag
     2221 tgactgcggc gtcgccgtgg ccatagggca ggcggcggca gcgatgccgc cgccgcaccc
     2281 ttgttatccc tcgggccgcc tgccgctcag ttgagaacga gaacgccgag attaagggtc
     2341 gaggcgtagg ccagccagac gatatagggg gcgagcagat acccggcccg gcgatcgtgt
     2401 ttgcggaaga ggaccatggt gatgatcacg gcggcgagca gcgccaggat gtcgaccagc
     2461 gccaacccca cccactgccg gccgaaaaag aggtaggtcc agacgccgtt gagcgccagt
     2521 tgcgccagga agaggacgag cgccgcccgc gccccggcga aaccatcgcg ccgccacacc
     2581 a
//
LOCUS       DAOOKS010000255         2574 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_49508_length_2574_cov_2.736006, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000255 DAOOKS010000000
VERSION     DAOOKS010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49508_length_2574_cov_2.736006"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1074..>2574
                     /locus_tag="PK112_04950"
     CDS             1074..>2574
                     /locus_tag="PK112_04950"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HPC11430.1"
                     /translation="MSDNDSSLAGRAAVAMALTIGFYSLALILGLGTILLPFIIAWAT
                     GQLILKLVIVCLISGGAILAAIVPRRDQFEPPGPELSLAEHPRLAEVIRRVAGQTEQA
                     MPRTVYLIPDFNAWVAQRGGSHLHGGDRLMGIGLPLLHQLTVSQFEAVIAHEFGHYHH
                     GDTRLGPRIYQTRAAIVRTIAGLHEHNSILQAPFLWYGRMFLQITHAISRRQEFNADR
                     LAAQVAGTAAMRSALEKINRGGIAFDAYWQSEFVPMLRSRRLPPLLDGFTLFLQCEPM
                     QTRIDACAEQVLASEATDKYDTHPSLRERLAALEALPAGPALSDRRPALDLLSDPAEQ
                     ERELLRAIVAAGEFERLTPVGWDEVAPIHAARWQAMSRERRHFFFAITPKDLPRFVRD
                     PKPFVKDRPLQDLLAGPNQAQAAEAVAVTVALALASLLVRLGWTLERPIGSNPRMVLG
                     AAIIEPFDTLDLLRSGQLPAPEWLRLCRDTGIGDHPLFPPSHKEDGARAE"
BASE COUNT          416 a         1028 c          665 g          465 t
ORIGIN      
        1 aaggtagccg tacttcgcct gcatgtcctc gagtatcgaa atgagactgc cgcgattccc
       61 gtggtgcttc tccacgatgc tgaggacttc ctgtgtgttc atggcctgcc cttctgcttc
      121 acatcgatcg tccgccgccg caggcgcgcc cggccgccct gagcgcagtc gatgggcgac
      181 cccttcgccc gcccgccgcc gtcctccggc cgcgggcagc ttccgaccga catcgatcta
      241 tgttcatgtg tattcctcgc aatgccagat ccccgcctca ggacgcggat gcgcacaggt
      301 ggccgctccg gcgcaggtcg cacacaatcc gcgcgccccc ccgtcgccgc cggtatcgtt
      361 ctcacgcgcg gacggctgac cctccggtcc gtccccaaat gcccggtgct cgaacatctc
      421 gcagtacaac gcgtccaccc cccgcttctc ccgaaagaca cacccgcggc ggaacttgca
      481 cgtcgggcac agccccgccg gcgccgtccg ctcacccgac cacacaaccc gcccctccgc
      541 cctgtacggg gaaaaccgtc ccacccgacc atccctgtcg cactcccgac atcgtcgcat
      601 tctgggtcaa tttctccata catcacaccc tcccctatca gattgcatgc caagtcgctg
      661 tcgcgctcgc tttctctgaa gacccgcaca ccaccgtcca acccggcatc gtcgcacttg
      721 cttgattcgc atgctgataa ttgtcatttc gggaatcgca ttcgacgatt caaaaagtct
      781 atattaccac cgctcaacca ctaaccgtta tcggggaatt tctccccacc ggatcatttt
      841 tcctcgatga tgacaggaga tctcacgccc gaggccgagc actccacgcc ccctcctcct
      901 ccccgcccca accctccaaa atccctccca ccccatcaat tctcgttgac acccggctcc
      961 tctgggggct attgattcat agattgcagg gccggccgat cttcaaggcg acgctttcct
     1021 gcgggtgcgg acgacaccgg aaacggtccg ccgacccttt ggatggaggg actatgtcgg
     1081 acaacgattc atctctggcg ggtcgcgccg ccgtggccat ggccctgact atcggcttct
     1141 actcgctcgc actcatcctc ggcctcggca cgatcctgct gccgttcatc atcgcgtggg
     1201 ccaccggcca gttgattctg aaactggtca tcgtctgcct catctccggc ggcgcaattc
     1261 tggcggccat cgtcccgagg cgggaccagt ttgaaccgcc cggtcccgaa ctgagtctcg
     1321 ccgagcaccc ccggctggcc gaagttatcc gccgcgtggc cggtcaaacg gagcaagcca
     1381 tgccccgcac cgtctacctg attcccgatt tcaacgcctg ggtggcccag cgcggcggct
     1441 cgcaccttca cggcggcgac cgcctcatgg ggatcggcct tcccctgctc caccagttga
     1501 ccgtctcgca gttcgaggcc gtgatcgccc acgaattcgg ccactatcac cacggcgaca
     1561 cgagactcgg cccccgcatc taccagaccc gggccgccat cgtccgcacc atcgccggcc
     1621 tgcacgagca caattccatc ctccaggcgc ccttcctctg gtacggcagg atgttcctgc
     1681 agatcaccca cgccatttcc cgccgccagg aattcaacgc cgaccggctc gccgcccagg
     1741 tggccggcac tgccgccatg cgctccgccc tcgagaaaat caaccggggc ggcatcgcct
     1801 tcgatgccta ctggcagagc gagttcgtgc ccatgctccg gtcccgccgc cttccgccgc
     1861 tgcttgacgg ctttactctc tttctccagt gtgagccgat gcagacccgg atagacgcct
     1921 gcgccgagca ggttctcgcc tccgaggcca ccgacaagta cgacacccac ccctcgctgc
     1981 gcgaacggct cgccgccctt gaggccctcc ccgccggacc cgcgctctcc gatcgccggc
     2041 ccgcgctcga cctcctctcc gaccccgccg aacaggagcg ggaactcctc cgcgcgatcg
     2101 tcgccgcagg cgaattcgaa cggctcaccc cggtgggctg ggacgaggtc gctcccatcc
     2161 acgccgcccg ctggcaggcc atgagccgcg agcgccggca cttcttcttc gccatcaccc
     2221 ccaaggacct cccgcggttt gtccgggatc ccaagccctt cgtgaaagac aggcccctgc
     2281 aggatctgct cgccggcccc aatcaggccc aggcagcgga ggcggtcgcc gtcaccgtcg
     2341 ccctcgccct cgcctccctc ctcgtccgcc tcggctggac gctcgagagg ccgatcggca
     2401 gtaacccccg catggtcctc ggagccgcca ttatcgaacc cttcgacacc ctcgatctcc
     2461 tcaggtcggg acagctcccc gcgccggagt ggctccgcct ctgccgcgac accggaatcg
     2521 gcgaccaccc cctcttcccg ccctctcata aagaggatgg cgcgcgggcc gaat
//
LOCUS       DAOOKS010000256         2573 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_49547_length_2573_cov_2.775218, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000256 DAOOKS010000000
VERSION     DAOOKS010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49547_length_2573_cov_2.775218"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            50..982
                     /locus_tag="PK112_04955"
     CDS             50..982
                     /locus_tag="PK112_04955"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPC11431.1"
                     /translation="MQLTLDHIRKEFDGKVAVNDLSLEVPEGVIYGIIGPNGAGKTTT
                     IRMVMNITVPDRGRVLVDGRTAGPDFNNRVGYLPEERGLYKKMTLEEVILYMAELKGV
                     GRREMLPKIGPWLERMNLAAYRHRKVEELSKGMAQKLQFVTTIIHEPEIVILDELFSG
                     LDPINIELIKDILLEIKRSGRTILFSTHVMEQAEKLCDHVCMIAGGEKVLDGPLGRVK
                     AQFGENTIQLEIEGDALFVGELPGVRNVTQFTNYIEIDLGDGARPNEILRAVTDRVPV
                     RRFQIIEPSLYSIFIRMARPESDAAAAGGEAAHA"
     gene            975..2285
                     /locus_tag="PK112_04960"
     CDS             975..2285
                     /locus_tag="PK112_04960"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPC11432.1"
                     /translation="MRKFWVVFKREYAQVVKKKSFLIGVLLTPALMAGFVVLPMLLAD
                     PGLDRPQSIAIIDQSSAGFGERLQERLVEVKIKETEAPLYDFRGFFTVAPGDDPGYRR
                     VLDSLTAAINAQELKYVVVIRPNPQLSDTGVFAVTNATNFTILQTIENHASQLLSMER
                     LRQSHVNVSVDSVLALTRRLDLQVRDAKGETMPFEYKYFGALIFVMVMFGMILGYGQM
                     VMRSVIDEKNSRIMEVLISSVTPFQLMLGKVLGLGAATFTQAAIWVALGAVLYANKAA
                     LSVDSSIDRLLFNPVIVVFFVLYLTTGYLLFSTLFALVGSIVNSEKEAQNFVFPITMS
                     LVLPVMIGIHVVQEPNSTLSLVLSYIPFLTPTMMMMRIIFIAPTMTEYSLFGGIVGQA
                     VLGFVLVSLTTVAVIWLTAKIFRVGILMYGKRPTLPEIVKWVRY"
     gene            2317..>2573
                     /locus_tag="PK112_04965"
     CDS             2317..>2573
                     /locus_tag="PK112_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11433.1"
                     /translation="MIVKRTTLPSVLIMLAAFVFFSANTVYFLEAEDGPGLSPLQRNY
                     QFDFSTVREGPRLKTKAAILVDYENGRVLYAKEADEVRPEI"
BASE COUNT          499 a          775 c          815 g          484 t
ORIGIN      
        1 gaccgggcgg aatgcgtatc caaagccatt gtgagaccag gagaagcgga tgcagttgac
       61 gctcgatcat attcgcaagg aatttgacgg caaggtggcc gtcaacgatc tctcgctcga
      121 agtgcccgag ggggtgatct acggcatcat cgggcccaac ggggcgggca agacaacgac
      181 catccgtatg gtcatgaata tcaccgtccc ggaccggggg cgggtgctgg tcgacggccg
      241 cacggccggt cccgatttca acaaccgcgt cgggtatctt cccgaagagc ggggactgta
      301 caagaagatg acgctcgaag aggtcatcct ctacatggcc gagctcaaag gggtcgggcg
      361 gcgcgagatg ctgccgaaaa tcggcccctg gctggagcgc atgaacctgg cggcctaccg
      421 ccaccgcaag gtcgaggagc tgtccaaggg aatggcgcag aaactccagt tcgtcaccac
      481 catcatccac gaaccggaaa tcgtcatcct cgacgagctg ttctccggcc tcgacccgat
      541 caacatcgaa ctgatcaagg acatcctcct ggagatcaag cgctccggcc ggacgattct
      601 cttttcgacc cacgtgatgg aacaggccga gaagctgtgc gaccacgtgt gcatgatcgc
      661 gggcggggag aaggtgctcg acggtcccct gggccgggtg aaagcgcagt tcggcgagaa
      721 cacgatccaa ctggaaatcg agggggacgc cctctttgtg ggagagctgc cgggggtcag
      781 gaatgtcacc cagttcacca attacatcga aatcgatctg ggggacggag cgcggcccaa
      841 cgagattctc cgggcggtga ccgaccgcgt gccggtgcgg cggtttcaga taatcgaacc
      901 ttccctctac agcatcttca tccgcatggc ccggcccgaa tcggacgcgg ccgcagccgg
      961 cggggaggcc gcccatgcgt aaattctggg tcgtcttcaa gcgcgagtac gcgcaggtgg
     1021 tcaagaagaa atcgtttctc atcggcgtgc tgctcacgcc ggcgctcatg gccgggttcg
     1081 tggtgctccc gatgctgctg gccgacccgg ggctggaccg gccccagtcg atcgccatca
     1141 tcgaccagag cagcgccgga ttcggcgagc ggctccagga gcggctggtc gaggtcaaga
     1201 tcaaggaaac cgaagccccc ctgtatgact tccgcggctt tttcacggtc gcccccgggg
     1261 acgacccggg ctaccggcgg gtgctcgatt cgctcaccgc ggcgatcaac gcccaggaac
     1321 tcaaatacgt ggtggtgatt cggccgaacc cccagttgtc cgacacgggc gtatttgcgg
     1381 tcaccaacgc caccaatttc accatcctcc agacaatcga gaatcacgcc tcgcagcttt
     1441 tgtcgatgga gcggctgcgg cagtcccacg tcaatgtgag tgtcgattcc gttctcgccc
     1501 tgacgcgccg gctggatctc caggtgcggg acgcgaaggg ggagacgatg ccgttcgagt
     1561 acaagtattt cggcgcgctc atcttcgtca tggtcatgtt cgggatgatt ctcggatacg
     1621 gccagatggt gatgcggagc gtgatcgacg agaagaactc gcgcatcatg gaagtcctga
     1681 tctcctcggt caccccgttc cagcttatgc tcgggaaagt gctggggctg ggggcggcga
     1741 ccttcacgca ggcggcgatc tgggtggcgc tcggcgccgt gctgtatgca aacaaggccg
     1801 cgctcagcgt cgacagctcg atcgaccggc tcctgttcaa cccggtcatc gtggtcttct
     1861 tcgtgctcta cctgacgacc gggtacctgc tgttctcgac gctcttcgcg ctggtcggat
     1921 cgatcgtcaa ttccgagaag gaagcgcaga atttcgtgtt cccgatcacc atgtcgctcg
     1981 tgctgccggt catgatcggg atccacgtcg tgcaggaacc caactcgacg ctgtcgctgg
     2041 tgctctcgta cattccgttt ctcacgccca cgatgatgat gatgcggatc atcttcatcg
     2101 ccccgaccat gaccgagtac tcgctcttcg ggggaatcgt cggccaggcg gtgctcggct
     2161 tcgtgctggt gtcgctgacg acggtggcgg tgatctggct gacggcgaag atattccgcg
     2221 tgggaatcct gatgtacggc aagcggccga cgctgccgga gatagtcaaa tgggtgagat
     2281 actagcggca accgacagag cggagacacc tggggaatga tcgtcaagcg cactaccctg
     2341 ccgagcgtgc tgatcatgct cgccgcattc gtgttcttct cggccaacac cgtctatttc
     2401 ctggaggccg aggacgggcc ggggctttca ccgctccagc gcaactacca gttcgatttc
     2461 tcgaccgtgc gggagggacc gcggctcaag accaaggcgg cgatcctggt cgactacgaa
     2521 aacggccggg tgctctatgc gaaagaggcc gacgaggtcc ggccggagat cga
//
LOCUS       DAOOKS010000257         2567 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_49711_length_2567_cov_2.070860, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000257 DAOOKS010000000
VERSION     DAOOKS010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49711_length_2567_cov_2.070860"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(141..788)
                     /locus_tag="PK112_04970"
     CDS             complement(141..788)
                     /locus_tag="PK112_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11434.1"
                     /translation="MRREVPLVITFVVGLVFVVQYFIPHFPFNRLEGWFSEWFSIVGA
                     FAIWLGALNLMKLSIQSVARRRGDWWYSVVVIASFLLVAVIGFSAGSDYRDPGTSFDW
                     LYVNIYNPLSSTMYAILAFFVASASYRAFRARNLEATLLLVAAFLVMLGRVPVGDVLT
                     GFLPEGWRASAWADAIMTYPQTAGQRAIMIGIALGIVSTALRIILGIERAHLGGD"
     gene            complement(791..2038)
                     /locus_tag="PK112_04975"
     CDS             complement(791..2038)
                     /locus_tag="PK112_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HPC11435.1"
                     /translation="MFALVLTASAPAPAIAQALSDSSATDSVTVGADGAVTALRPAPP
                     GSIEAKDTPNDAGGSITVRWVLSPDDAAGGVTAYRVLRAEAQDGRPGEFTEIGGATAG
                     QDLYQDGNVSDDRQYFYKVVAANTVRLNGATLAVAEAESAPVGPVRSSAQWFNLGRIN
                     VFVGVLLLSGFIVYYIRRAQAGKRLFIRKIAGLEAVDEAVGRATEMGKKIFYVPGISD
                     MDNVQTIAAVTILSRVAEMSAQYEARLDVPVARSLVMVTSKEVVKEAYLKAGRPDSFH
                     EDQVHYLTDDQFGYAAGIDGMVTRERPATIFYMGQFFAESLVLAETGNSIGAIQIAGT
                     AELAQLPFFVASCDYTLIGEELFAASAYLSREPRLLGSLKGQDIGKAIILIALIIGLL
                     LETFGNPLFGWDWHFSNLFSTGE"
BASE COUNT          522 a          859 c          811 g          375 t
ORIGIN      
        1 agaaccataa tgatggtcga cacgagaggc gaaaacgaca ccgcccccag ccgcccggcg
       61 ccccggccga ggtacacgat gaccgccgca gccgccagca ccgtcagcac gagaatctcc
      121 ttgaaacgcg tgctcataga tcagtctccc cccaggtgcg cccgctcgat cccgaggatg
      181 atccggagcg cggtggagac gattccgagg gcgatgccga tcatgatggc ccgctgaccc
      241 gccgtctgcg ggtacgtcat aatggcgtcg gcccaggccg acgcccgcca gccctccggc
      301 agaaaaccgg tgagcacatc ccccaccggc acccgcccga gcatgaccag gaaagccgcg
      361 accagcagga gggtcgcttc gagattgcgg gcgcgaaacg cccggtacga ggccgaggcg
      421 acgaagaacg ccaggatagc gtacatcgtc gacgagagcg ggttgtagat gtttacgtag
      481 agccagtcga acgacgtccc cgggtcgcgg tagtcggagc cggcggagaa gccgatgacg
      541 gcgacgagca ggaacgaggc aatgaccaca accgagtacc accagtcgcc ccgtcgccgc
      601 gccaccgact ggatcgacag cttcatcagg ttgagcgccc ccagccagat cgcaaaagcc
      661 ccgacaatgg aaaaccactc cgagaaccac ccctccagcc ggttgaaggg aaagtggggg
      721 atgaagtact ggacgacgaa gacgaggccg acaacgaagg tgatgacgag cggtacttcc
      781 cggcgcatga tcactccccg gtgctgaaca ggttggagaa atgccagtcc cacccgaaga
      841 gcgggttgcc gaaggtctcg aggaggaggc cgatgatgag ggcgatgagg atgatggcct
      901 tgccgatatc ctgccccttg agcgatccca gcagccgggg ctcgcgcgag aggtaggccg
      961 aggcggcgaa caactcttcc ccgatcaggg tatagtcgca ggaggcgacg aagaagggta
     1021 actgggccag ttcggccgtg cccgcaatct ggatcgcgcc gatcgaattg ccggtctcgg
     1081 cgaggaccag cgattcggcg aagaactgcc ccatgtagaa aatggtcgcc ggccgctcgc
     1141 gcgtcaccat gccgtcgatc cccgcggcgt agccgaattg gtcgtcggtc aggtagtgca
     1201 cctgatcctc gtggaaggag tccgggcggc ccgctttcag ataggcctcc ttgaccacct
     1261 ccttgctcgt caccatcacc agcgagcggg ccaccggcac gtccagccgc gcttcgtact
     1321 gggccgacat ctcggctacc cggctgagga tggtcaccgc ggcgatcgtc tggacattgt
     1381 ccatgtcgga gatgcccggg acgtagaata tctttttgcc catctcggtg gcccggccga
     1441 cggcctcgtc gaccgcctcc agcccggcga ttttccgaat gaagagccgc ttgccggcct
     1501 gcgcgcgccg gatgtagtag acgatgaagc ccgacaggag cagcacgccg acaaagacgt
     1561 tgatgcggcc gagattgaac cactgggccg acgaccgcac cggcccgacc ggcgccgact
     1621 cggcctccgc caccgccagc gtcgccccgt tgagccggac cgtgttagcc gcgacgacct
     1681 tgtagaaata ctgccggtca tcgctcacat tgccgtcctg gtacagatcc tgcccggccg
     1741 tcgcgccgcc gatttcggta aactcccccg gtctcccgtc ctgggcctcg gcccgcagca
     1801 cacggtaggc cgtcaccccg cccgccgcat cgtcgggcga cagcacccag cggaccgtga
     1861 tcgacccgcc cgcgtcattc ggcgtgtctt tcgcctcgat actgcccggc ggggccggcc
     1921 gcagcgcggt caccgcccca tcggccccga ccgtgacgga atccgtcgcc gaggagtcgg
     1981 aaagcgcttg ggcgatggcc ggcgccgggg cggaggccgt caggaccaga gcaaacacca
     2041 gagcgagagg gaaaaccgga ggaaagatcc agagagggcg tgtggggcga acgggcaatc
     2101 ttggctccct tcgggctgac ggtgcaaatc gacccgcgga cgaggccccg cgggcacacg
     2161 acccggtcga acggttgaac cggctgccgc cggcatccgg gcagcgcgcc ggcgctgggg
     2221 cacacaatta gcgttgaatc tagcccggcc ggcaggcgga gtcaagcgaa aagtaccctc
     2281 gcgcaatccc gggcgctctc cgcacacccc accgacgcct tcaaaaatcg ctctttacat
     2341 ccccgtcgcc gccgtgttat actgacgatc ttgacacggt ccgggcctcg gtgtgctctt
     2401 tcgcacccgt ccggcggcgt gtccgcaaga accgaaggga catattggcg agaaagatag
     2461 gtactctcga tggcgaaatt tgacaatccc gaggacatta gaatcatcgt tgccaccgac
     2521 tgcggatcca ccactacgaa agcaatcctc attcagtaca tcgacgg
//
LOCUS       DAOOKS010000258         2565 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_49762_length_2565_cov_3.307570, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000258 DAOOKS010000000
VERSION     DAOOKS010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49762_length_2565_cov_3.307570"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="PK112_04980"
     CDS             complement(<1..484)
                     /locus_tag="PK112_04980"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPC11436.1"
                     /translation="MPRDAAKVSVLHLRASNFYGGPERQLHCHARAARDSVFEVIVAS
                     YAESGRSPDLLARVAADRLPTHLVGVAGAYDRRALGLIRRVLRDRDIAILCTHDYRST
                     VLGCLAVRFTPVRWIAFSRGRTRETLRIRLYNELERLCLRFADRVVAVSEGEKASLRR
                     R"
     gene            complement(460..1680)
                     /locus_tag="PK112_04985"
     CDS             complement(460..1680)
                     /locus_tag="PK112_04985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPC11437.1"
                     /translation="MDETTSRLRLLLIDEEFPYPLTSGKRLRTYNLLRELSRRCDITT
                     LAYGDPDSEGFRRFERDGLTPVAVPAPDRRQSGLRFYWRLFGNLFSPYPYIVTSHTTR
                     RFHRRLRQLLARHSFDLVLCEWTPYARFVKDLPGVRKVITAHNIEAAIWRRYEQQETQ
                     PLRRLYIRLQRTKVEAFEARCFGWVDGAIAVSESEARQIAELGVPYPPAVVENGVDVG
                     YFTAPADDPDPGQIVFSGAMDWRPNQDAALWFVQEIFPLLRRARPNLAVTIVGRDPVR
                     RIRELGRVEGVTVTGTVADVRPYLARAAVFIVPLRIGGGSRLKILEAMAMRKAVVSTS
                     VGAEGLRVAADENIMIADDAPAFAQAVLRCLGEPDLRRRLGENGRRLVEREYRWDILG
                     HKMYEYLCRVMQQK"
     gene            complement(1841..>2565)
                     /locus_tag="PK112_04990"
     CDS             complement(1841..>2565)
                     /locus_tag="PK112_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11438.1"
                     /translation="AYPSLAYDLAAAIEAGDIEPFRVPMVNLTAEPVYDFQKQLIRAT
                     FGEEVCERYGSREMGLAALECPAHNGLHYFMESVYFESIDRGPGSPGRSLVVTDLVNR
                     GMPFIRYDTGDFARLDDAPCTCGRTTPRLIDIRGRETDNIWCPDGHGIAGAIVVYLFA
                     RINPGVPMQLMQESLDRIVIRIAGRPGELAGRAAELIDVCRREISPDIAYTIEYVDHI
                     ERAPSGKYRYIISRVPPPSRRR"
BASE COUNT          438 a          728 c         1008 g          391 t
ORIGIN      
        1 gacggcggcg gagggaggct ttctcgccct cggagacggc gacgacgcgg tcggcgaacc
       61 gcaggcacaa gcgctccagt tcgttgtaga gccgaatcct gagggtctcg cgcgtgcggc
      121 cgcgggagaa ggcgatccag cggacggggg tgaagcggac ggcgaggcag ccgaggacgg
      181 ttgagcggta gtcgtgggtg cagaggatgg cgatgtcgcg atcgcgcagg acccggcgga
      241 tgaggccgag ggcgcggcgg tcgtaggcgc cggcgacgcc gacgaggtga gtcgggagac
      301 ggtcggcggc cacccgggcc agcaggtcgg gggagcgacc ggactcggcg taggaggcga
      361 cgatgacctc gaagacggag tcgcgggcgg cccgggcgtg gcaatggagc tggcgttccg
      421 gaccgccgta gaagttgctc gcccgcaggt ggagaacgct cacttttgct gcatcacgcg
      481 gcataagtac tcgtacattt tgtgtccgag aatatcccat cggtactccc gttcgacgag
      541 gcggcggccg ttttcgccga gtcgcctgcg caggtcgggt tcgcccaggc accgcagaac
      601 ggcctgcgcg aacgccggag cgtcgtcggc gatcatgatg ttttcgtccg cggccacccg
      661 aaggccctcg gcgcccacgc tggtggagac aaccgccttg cgcatggcca tggcctcgag
      721 gatcttcagg cgcgagccgc cgccgatgcg cagggggaca atgaagacgg cggcccgggc
      781 gaggtaggga cggacatcgg cgaccgtgcc ggtgaccgta acgccctcga cgcgcccgag
      841 ttcccggatg cggcggacag gatcgcggcc gacgatggtg acggcgaggt tggggcgcgc
      901 ccggcggagg agcggaaaga tctcctggac aaaccagagc gcggcgtcct gattggggcg
      961 ccagtccata gcgccggaga agacaatttg accggggtcg gggtcgtcgg ccggggcggt
     1021 gaaataaccg acatcgacgc cgttctcaac caccgccggg gggtagggga cgccgagctc
     1081 ggcgatctgg cgggcttcgc tctcggagac cgcgatggcg ccgtcgaccc acccgaaaca
     1141 gcgcgcctcg aacgcttcca ccttggtgcg ctgaagccga atgtacagcc ttcggagcgg
     1201 ctgggtttcc tgctgttcgt agcggcgcca gatagcggct tcgatgttgt gggcggtgat
     1261 cacttttcgg acgcccggca ggtccttgac aaaccgggcg tagggggtcc actcgcacag
     1321 gaccaggtcg aacgagtgcc gggcgaggag ctggcggagg cggcggtgga aacggcgggt
     1381 ggtgtggctg gtgacgatgt acggataggg ggagaagaga ttgccgaaga ggcgccagta
     1441 gaagcggagg ccgctctggc gacggtccgg ggccgggaca gccaccgggg tgagaccgtc
     1501 gcgttcgaac cggcggaaac cctcggagtc aggatcgccg taggcgagcg tcgtgatgtc
     1561 gcagcggcgg gacagttcgc ggaggaggtt gtaggtgcgg aggcgcttgc ccgaagtgag
     1621 ggggtagggg aattcctcgt caatcagcaa gaggcggagg cgcgacgtgg tctcatccat
     1681 agctaccttc cggcgggcgc cgcgctcacc ggggcccgag tgcacaccgg ggatccggac
     1741 ggcgcctgca tgatcgtttc aacatactca aattcaaacg gaaagtccgg tccaatttta
     1801 cggttcttgg ccgccgggga gcgcgccggt cggggcggac tcagcggcgg cgggaggggg
     1861 gcggcacgcg cgagataatg tagcggtact tgccggaggg ggcgcgctcg atatggtcga
     1921 cgtattcgat ggtgtaggcg atgtcggggc tgatctcacg tcggcagacg tcgatgagtt
     1981 cggcggcccg cccggcgagt tccccgggcc ggccggcgat gcggataacg atgcggtcga
     2041 gcgactcctg catgagctgc atgggcacgc cggggttgat ccgggcgaag aggtagacga
     2101 caatggcgcc ggcgatgccg tggccatcgg gacaccagat gttgtcggtc tcgcggccgc
     2161 ggatgtcaat gaggcggggg gttgtgcggc cgcaggtgca gggagcgtcg tcgagccggg
     2221 cgaagtcgcc ggtgtcatag cggataaagg gcatgccgcg gttgaccaga tcggtgacga
     2281 ccaggctgcg gccgggactg ccgggtccgc gatcgatgct ttcgaagtac acgctttcca
     2341 taaaatagtg gaggccgttg tgggcggggc actcgagggc ggccagaccc atctcgcgcg
     2401 atccgtagcg ctcgcagacc tcctcgccaa aggtcgcccg aatgagttgt ttctggaagt
     2461 cgtagaccgg ttcggccgtg aggttgacca tcggcacgcg gaacggctcg atgtctccgg
     2521 cctcgatggc ggcggcgagg tcgtaggcga gcgacgggta ggcgg
//
LOCUS       DAOOKS010000259         2563 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_49823_length_2563_cov_2.833732, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000259 DAOOKS010000000
VERSION     DAOOKS010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49823_length_2563_cov_2.833732"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="PK112_04995"
     CDS             <1..234
                     /locus_tag="PK112_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011745681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HPC11439.1"
                     /translation="YFVMGDNRDDSRDSRFWGGVPAQNVKGKAVFVYWSWQPDDGAPG
                     FSFPYVIDAVQWIGYVVFNFPSHVRWDRVGLAL"
     gene            238..1377
                     /gene="hemW"
                     /locus_tag="PK112_05000"
     CDS             238..1377
                     /gene="hemW"
                     /locus_tag="PK112_05000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HPC11440.1"
                     /translation="MPFGLYVHFPFCRNTCSYCDFYKEKHQAALEREFYHALRIETEL
                     AAEHPAVQRSQVTTIFIGGGTPNISDPDLLTDWLGHLRRHIDVADEIEFTIECNPEAV
                     TREQLERLKGLGVNRPVFGVQSFNTRLLKLLDRRHHPHDSYRAIYYANALGFRAWGVD
                     FIFGLPGQTSKMLAADLDQLLDFEPPHVSYYQLTVEEGTPLWRRVKAGKLRMPDQELL
                     LALYRGGVEQMADLGYLRYEVSSFARPGFECRHNLGYWEGAEYLGLGPSAHSYLDNTR
                     FANWPNVREYIKALQAGILPRTVDESGAPERMTEAIMLGLRTARGISRSAFAERFGQP
                     IESRLDTEQYALLVESGHLIPERGMLRLSEEGIHLADEITRRLLK"
     gene            1460..1939
                     /gene="tadA"
                     /locus_tag="PK112_05005"
     CDS             1460..1939
                     /gene="tadA"
                     /locus_tag="PK112_05005"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018027240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HPC11441.1"
                     /translation="MDSDRYSIYMRLALREAEQAFEEGEVPVGAVVVQGDRVIGRGAN
                     RTVHLHDATAHAEMIALTAAANALGDWRLEGCELYCTLEPCAMCAGAAVLSRIERIVF
                     GSRDPKFGACGSIFLIPGDPRLNHRAEIVEGVLADAAAELMRAFFQRLRQAKGEEVT"
     gene            1936..2406
                     /locus_tag="PK112_05010"
     CDS             1936..2406
                     /locus_tag="PK112_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11442.1"
                     /translation="MKPLSETQERTWMVIRVVAAAVGIVAPAFFFAIAALVHPDSSPL
                     GENGAGAIDVAFYLLLILGIVDPLLALLIERPLLADWRRRPGRRTADRMFFHLAVLRM
                     AFVEATFLYGFVVYLLTWDLMRMLIFYLVGLVWLGLYWPRREKYEQFIEGAERL"
BASE COUNT          417 a          812 c          872 g          462 t
ORIGIN      
        1 tacttcgtca tgggggacaa ccgtgacgac agccgggaca gccggttctg ggggggcgtg
       61 cccgcccaga atgtcaaggg aaaggcggtg ttcgtgtact ggtcgtggca gccggacgac
      121 ggggcgccgg gattctcatt tccctacgtg atcgatgcgg tccagtggat cgggtatgtt
      181 gtcttcaact tcccgtccca cgttcgctgg gaccgtgtcg gactggcgct ctgaggcatg
      241 ccgttcggcc tgtacgtaca tttccccttt tgccgcaaca cgtgctccta ctgcgacttt
      301 tacaaagaga agcaccaggc cgccctggaa cgcgagttct accacgcgct gcgcattgaa
      361 acggaactcg ccgccgagca cccggcggtg cagcgcagcc aggtcacgac catctttatc
      421 ggcggtggca cgcccaatat ctccgatccg gatctcctga ccgactggct cggccacctg
      481 cggcggcata tcgatgtcgc cgacgaaatc gagtttacca tcgagtgcaa ccccgaggcg
      541 gtgacgcggg agcagctcga gcggctcaaa gggctgggcg tgaaccggcc ggtgttcgga
      601 gtgcagtcgt tcaacacgcg gctactgaaa ctgctcgacc ggcgccacca cccgcacgac
      661 agctaccggg cgatttacta cgccaacgcg ctggggttcc gcgcctgggg agttgatttc
      721 atcttcggac tccccggcca gaccagcaag atgctggcgg ccgatctcga ccaactgctc
      781 gatttcgaac ccccccacgt gtcgtactac cagctcacgg tggaagaggg gacgcccctg
      841 tggcggcggg tgaaggcggg gaaactgcgg atgcccgacc aggaactgtt gctggcgctt
      901 taccgcgggg gcgtggaaca gatggcggac ctcgggtacc tgcggtacga ggtctcctcg
      961 ttcgcccgac cggggttcga gtgccggcac aacctcggct actgggaggg ggcggagtac
     1021 ctcgggctgg ggccctcggc ccattcctac ctcgacaaca cccggttcgc caactggccg
     1081 aacgtgcgcg agtacatcaa ggcgctccag gccggcattc tcccccgcac ggttgacgag
     1141 tcgggggcgc cggagcggat gaccgaggcg atcatgctcg gtctgcgcac ggcccgcggg
     1201 atcagccgct ccgccttcgc cgaacgcttc ggccagccta tcgaatcgcg gctcgatacg
     1261 gagcagtacg cgctcctggt cgagtcgggg catctgattc ccgaacgggg gatgctgcgt
     1321 ctgtcggaag agggcattca cctcgccgac gaaatcaccc gacggctgct gaagtgacgg
     1381 cgtcgcgccg ggccgcctcc cccccggtcg gccgacaaag gggttgccgc gggtaatcca
     1441 acggatctat attattcaga tggactcgga ccggtacagc atatatatgc ggctggcgct
     1501 gcgcgaggcg gagcaggctt tcgaggaggg ggaggtgccg gtgggcgcgg tggtggtgca
     1561 gggggaccgc gtgatcggcc ggggggccaa ccgcaccgtc catctgcatg acgccacggc
     1621 gcacgcggag atgattgcgc tgacggcggc ggccaacgcg ctcggcgact ggcgcctcga
     1681 aggctgcgag ctgtactgca cgctcgagcc gtgcgccatg tgcgccgggg ccgccgtact
     1741 gtcccggatc gaacgaatcg tgttcggatc gcgcgacccc aaattcggcg cctgcggttc
     1801 gattttcctg atccccggcg atccccggct gaaccaccgg gcggaaattg tcgaaggcgt
     1861 cctggccgac gcggccgcgg aactgatgcg ggcgtttttc cagcgcctcc gccaagccaa
     1921 gggagaagaa gtgacgtgaa accgctgagc gagacgcagg agcggacgtg gatggtcatt
     1981 cgggtggtcg cggcagcggt cgggattgtc gcgccggcgt tcttcttcgc cattgccgcg
     2041 ctcgtccacc cggattcgtc gccgctcggc gaaaacggcg cgggggcgat tgacgtcgcg
     2101 ttctacctcc tgctgatcct ggggatcgtc gatccgctgc tggcgctgct gatcgagcgg
     2161 cccctgctgg ccgactggcg ccgccggccc ggccggcgca cggccgaccg gatgttcttc
     2221 catctcgccg tcctccgcat ggccttcgtc gaggcaacgt tcctctacgg gtttgtcgtg
     2281 taccttctga cctgggatct gatgcggatg ctcattttct acctggtcgg actcgtgtgg
     2341 ctcggcctct attggccgcg gcgggagaaa tacgaacagt ttatcgaagg ggcggagcgg
     2401 ttatgagaga cgaagggaac gaagatcggt acgatctggc gcgcatgatc gccgggtcgc
     2461 tctaccaggg gctgttcatc aacatcctgc tgcccgccgg gctgctgctg gcggcctatg
     2521 tcatcgagca gcgcggcggc ctgggcaacg cgctcggggc ggc
//
LOCUS       DAOOKS010000260         2544 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_50343_length_2544_cov_4.616714, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000260 DAOOKS010000000
VERSION     DAOOKS010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50343_length_2544_cov_4.616714"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..848
                     /locus_tag="PK112_05015"
     CDS             <1..848
                     /locus_tag="PK112_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11443.1"
                     /translation="PADCPGPGRYFQYPLYPDSSCYFTPHGFNAVPLRDSTEPPSSSL
                     TRIRKTYPDLPDPRTLPPDSLTSDMYTEDGYLKFYEYAGDIPGLLPSVPYWVAVTAFD
                     FGSPESCVRPLESAIAETAVSGFPLHAFDAPLSGSNQVYIYPNPYRTDAGYRVQGFEG
                     RAQEDRWDERVRAIWFANLPPQCTIHIYTLNGDRVRTLDHDTNPADPLHTRHAWDLIN
                     RNFQKVESGLYYWVVEVPGQPAQIGKLAIIREPRLSAARESPDSRRAFCLPESPFPFI
                     LAPVP"
     gene            845..1582
                     /locus_tag="PK112_05020"
     CDS             845..1582
                     /locus_tag="PK112_05020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HPC11444.1"
                     /translation="MKADIGRNIVELRGRIVEACEQYDRDADDITVVAITKTHPPSVI
                     RTAVAAGLHNIGESRVQEADAKIAALGQIARFHMVGHLQTNKVKRAVELFDVIQSVDT
                     LKLADEINRQAGKAELEIECLVQVNCSGEDQKYGVSPDACLDLCRQIRNLENIVLSGL
                     MTIGPLADDEDSVRAAFRRCRELFRQGQDLFGDEFDTLSMGMSGDFPLAIAEGSTMLR
                     IGSLLFGQRPTDEFSYTEDRESDKPSD"
     gene            1803..>2544
                     /locus_tag="PK112_05025"
     CDS             1803..>2544
                     /locus_tag="PK112_05025"
                     /inference="COORDINATES: protein motif:HMM:NF016959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HPC11445.1"
                     /translation="MSTALSPNDIRNYEFPNQMRGYDKDEVRELLQQVAAALEDLKQE
                     NLKIAIERDTVRTQLEALKQFEDTIKSAAIDARRNADTLVANAKREVGEILNKARAEA
                     ETIVGSRADEARVLDEQITRLRMVKVSYQTKLKNLIESHLELIDELQRVKEPEVPRMQ
                     PARPTAAAEPSADDTAKTPTITSIPATPAASETEPARAAGDFRRDLRTATTAPEADWI
                     RQAREANIPAVVPPTLRPFKPTNGLEVEE"
BASE COUNT          484 a          942 c          699 g          419 t
ORIGIN      
        1 acccggctga ttgccccggc ccgggccgct actttcagta ccctctctac cccgactcct
       61 cctgctactt caccccccac ggcttcaacg ccgtccccct gcgcgactcg accgagcctc
      121 cctcttcttc cctcacccgc atccggaaaa cctatcccga cctccccgat ccccgcaccc
      181 tcccgcccga ctccctcacc tccgacatgt acaccgagga cggctacctc aagttctacg
      241 agtacgccgg tgatatcccc ggcctcctcc ccagtgtccc ctactgggtc gctgtcaccg
      301 ctttcgactt cggctccccc gaatcatgcg tccgccctct ggagtcggcc atcgccgaaa
      361 ccgctgtgag cggcttcccc ctccacgctt tcgacgcccc cctctccggg agcaatcagg
      421 tctacatcta ccccaacccc taccgcaccg acgccggcta ccgcgtccag gggttcgagg
      481 gacgggcgca ggaggaccgc tgggacgagc gcgtccgcgc gatctggttt gccaacctcc
      541 ccccgcagtg caccatccac atctacaccc tcaatggcga ccgggtgcgc accctcgacc
      601 acgacacgaa tcccgccgat ccgctccaca cccgccacgc gtgggatctc atcaaccgga
      661 acttccagaa agtcgagagc gggctctact actgggtggt cgaggttccc ggccaaccgg
      721 ctcagatcgg caaactcgcc atcatccgcg agccccgcct ttccgcggcg cgggagtccc
      781 cggactcccg ccgcgctttt tgcttgcctg aatccccctt tcccttcatc ttggcgccgg
      841 taccatgaaa gcggacattg gccgaaatat cgttgagctg cgcggacgaa tcgtcgaggc
      901 ctgcgaacaa tacgaccgcg atgccgacga catcaccgtg gtcgccatca ccaaaaccca
      961 ccctccgtcc gtcatccgca ccgcggtcgc cgccggcctg cacaatatcg gcgagagccg
     1021 ggtccaggag gccgacgcga aaatcgccgc cctcggccag atcgcccgtt tccacatggt
     1081 cggccatctc cagaccaaca aagtcaaaag ggctgtcgaa cttttcgacg tcatccagtc
     1141 ggtcgacacg ctgaaactgg ccgatgagat caaccgccag gccggcaaag ccgaactcga
     1201 aatcgaatgc ctcgttcagg tcaactgctc cggggaagac cagaaatacg gcgtttcgcc
     1261 ggacgcctgt ctcgacctct gccggcagat ccgaaatctg gagaacatcg tcctctccgg
     1321 cctcatgacg atcggcccgc tcgcggacga cgaggacagc gtgcgcgccg cattccggcg
     1381 ctgccgcgag ttgttccggc agggacagga tctgttcggc gacgaattcg acacgctctc
     1441 gatgggtatg tcgggcgatt ttccgctggc gatcgccgag ggctcgacca tgctgcgcat
     1501 cggcagcctt ctgttcgggc agcgtccgac cgatgaattt tcgtacacgg aggaccggga
     1561 gtccgacaag ccgtccgact gagctgtcta tctctctgtc ctgcttgcgc atacaacctg
     1621 ctccgactct gcggcaggcg gccgtcccgc cttctcaagt cccattccgt tctcaatttt
     1681 catcccccgt ctcccgataa ctaatacaga tacagaccgt accggcccgg tggctgccgg
     1741 aaaggcatct ttccgttccc ggcgccggtg gactggaaaa gggaaactga aggagctgac
     1801 ccatgagtac ggccctgtcg ccgaacgata tccgcaatta cgagttcccg aaccagatgc
     1861 ggggatacga taaggatgaa gtgcgcgagc tcctgcagca ggtggccgcg gccctggagg
     1921 atctcaagca ggagaacctc aagatcgcga tcgagcggga cacggtccgc actcagctcg
     1981 aggctctcaa gcagttcgag gacacgatca agagcgcggc tatcgacgcc cgccgcaacg
     2041 ccgacactct cgtggccaac gccaagcgcg aagtggggga gatcctcaac aaagcccgcg
     2101 ccgaggccga gaccatcgtc ggctcccgcg ccgacgaagc ccgtgtcctc gacgagcaga
     2161 tcacccgcct gcgcatggtc aaggtctcgt accagaccaa gctcaagaat ctcatcgagt
     2221 cgcacctcga gttgatcgac gaactccagc gggtgaagga gccggaggtg ccgcggatgc
     2281 agccggcgcg cccgaccgcc gccgccgagc cgtcggccga cgataccgcg aagactccga
     2341 ccatcacgag cattccggcg accccggccg cctcggagac ggagcccgcc cgggccgccg
     2401 gcgatttccg ccgcgatctc aggacggcga cgaccgctcc ggaggcggat tggatccggc
     2461 aggcgcgcga ggccaacatc cccgccgtcg tgccgcccac cctgagaccg ttcaagccga
     2521 ccaacggcct tgaggtcgaa gaag
//
LOCUS       DAOOKS010000261         2531 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_50701_length_2531_cov_3.030695, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000261 DAOOKS010000000
VERSION     DAOOKS010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50701_length_2531_cov_3.030695"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="PK112_05030"
     CDS             <1..181
                     /locus_tag="PK112_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11446.1"
                     /translation="GTVAVSDLTFAIAYLFRGGVSAGCPAHMDVNGDGSPAVSDLTLL
                     IGYLFRGGPVPAACP"
     gene            complement(255..1202)
                     /locus_tag="PK112_05035"
     CDS             complement(255..1202)
                     /locus_tag="PK112_05035"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HPC11447.1"
                     /translation="MRKYLLTPLVLSLCLSAPRAQDWVPVTLPTGDNITGIEFLHPDT
                     GFLSIQPGDLGRTYDGGRTWTIHPVALDLALEDVSFANAKLGLVCGKAGALYRTSDGG
                     ERWESVSPVGRDSTVAFFSVKFIDPRTALVTGMSRHEETPWSGIAYRSLDGGATWTRL
                     EPMGLMYSEIFYSPTAGLVFPALGLLNRSDDKGKSWRQNGAIESALNRSVAVASDLIV
                     LVGPKGFLAVSRDNGKTWKKIEQDVERTFVAVTAIDDRTVFVAGPNSLMLRSSDGGET
                     WTQELLARSFDVLDLARAGSRLYAVGTDGAVIYKELGRK"
     gene            1334..1999
                     /locus_tag="PK112_05040"
     CDS             1334..1999
                     /locus_tag="PK112_05040"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPC11448.1"
                     /translation="MPDTAIVELSNVDLAYPRSGELFRNLSLSVRPGETAVITGGPGS
                     GKTTIAELLVGRKFADAGSVEVFGENLRRGRGRALNRVRRRLGGVGGIFGLVPTYTAA
                     DNITYPLVLAGVRKRARKERLLRMLTEFSLLTRAQDYPATLTRVEQTLVQLARASIAD
                     QPLLVIDEPMAGLDTATARRVFEYLVKASLSGRTMVLLMSDHPPGTLPNSTSYVIREG
                     RLT"
     gene            1996..>2531
                     /locus_tag="PK112_05045"
     CDS             1996..>2531
                     /locus_tag="PK112_05045"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HPC11449.1"
                     /translation="MTRTAHIAGEFARSLGRHPFTALGSLLSLTLLYLLFDLYWIGAA
                     TVEQVFARAVAEVQMEVFVREEVAEGEVAGVQAEVAAIPGVTGHQYISKEAAREIMQA
                     SLGMDLLAGYDTLNPLPRSFVLTFAPAALTSDGLAAIAGELRRMPAVADLYYNAEWLR
                     KTEGTQAVILRVGLALGI"
BASE COUNT          393 a          773 c          926 g          439 t
ORIGIN      
        1 cgggacggtg gcggtgtcgg atctgacgtt cgcgatcgcg tacctgttcc ggggcggagt
       61 ctcggcgggc tgccccgccc acatggatgt gaacggggac ggttcgccgg ctgtgtccga
      121 cctgaccctc ctgatcggct atctcttccg cggcggcccg gtgccggcgg cctgtccgta
      181 agggaccggg agccggggcg gttcgggtgc cattcgatgg cgggcgcggc ccggcggccg
      241 ggcccgcagc taccctactt gcggccgagt tccttgtaga tcacggcgcc gtcggtaccg
      301 acagcgtaga gacggctgcc cgcccgcgcc agatcaagga cgtcgaacga gcgggccagc
      361 agctcctgag tccacgtctc gccgccgtcg gacgagcgca gcatgagcga gttgggtccg
      421 gcgacgaaaa ccgtgcggtc gtcgatcgcg gtgaccgcga caaacgtgcg ttcgacgtcc
      481 tgctcgattt tcttccacgt cttgccgttg tcgcgggaaa cggcgaggaa acctttcggg
      541 ccgacgagga cgatcaggtc gctggcgacg gcgacgctcc ggttgagggc gctctcgatc
      601 gcgccgttct gacgccacga tttccccttg tcatcggacc ggttgagcag gccgagcgcc
      661 ggaaagacca gcccggcggt cggggaatag aaaatctcgc tgtacatgag acccatgggc
      721 tcgagtctgg tccaggtcgc cccgccatcg agagagcggt aggcgatgcc gctccagggg
      781 gtctcctcgt gccggctcat gccggtgacc aacgcggtgc gggggtcgat gaatttgacg
      841 ctgaaaaaag cgaccgtgct gtcccgcccg accggggaga cgctctccca gcgttcgccg
      901 ccatcggagg tgcggtacag ggcgccggct ttgccgcaga ccaggccgag tttcgcgttc
      961 gcgaacgaga cgtcttcgag cgccaggtcg agggcgaccg ggtggatcgt ccaggtgcgg
     1021 ccgccgtcat aggtgcggcc gaggtcgccc ggctgaatgg agagaaagcc ggtgtcgggg
     1081 tggaggaact cgatgccggt gatgttgtcg ccggtgggca aggtgacggg gacccagtcc
     1141 tgcgcgcgcg gggccgatag gcacaggcta agcacaaggg gagtaaggag atattttctc
     1201 atacgttgtt ttagttgagg ccgcttcttt gtctttgtac catcgactcc ggtataagtt
     1261 ttggcgtcga acggccgcag tatacggaac ttcgaggtta tcgtcaacgg tcgttcgaac
     1321 ggatggagtt ggtatgcccg acaccgcgat cgtcgaactg agcaatgttg atctcgccta
     1381 tccccgcagc ggagagcttt tccgcaatct gagcttgtcg gtgcgaccgg gggagacggc
     1441 ggtgattacg ggggggccgg gttcgggcaa gaccacgatt gcggaactgc tcgtggggcg
     1501 gaagtttgcg gacgcggggt cggtcgaggt gttcggcgag aatctccgcc gcggacgggg
     1561 gcgggcgctc aaccgggtgc ggcgtcggct tggcggcgtc ggcggcattt tcggcctcgt
     1621 gccgacctac acggcggccg acaacatcac ttacccgctg gtgctggcgg gcgtgcgcaa
     1681 gcgggcgcgc aaagaacggc tcctgcgcat gctcaccgag ttctcgctct tgacccgcgc
     1741 gcaggattac ccggcgacgc tcacgcgggt ggagcaaacg ctcgtccaac tggcgcgggc
     1801 ctcgatcgcc gaccagccgc tgctggtgat cgacgagccg atggcgggac tcgacacggc
     1861 gacggcgcgc cgggtgttcg agtacctggt caaagcctcg ctgtcgggcc ggacgatggt
     1921 tctcctgatg tcggaccatc cccccggcac cctgcccaac agcaccagct acgtgatccg
     1981 ggaggggagg ctgacgtgac ccggacggcc cacatagcgg gtgaatttgc gcggagcctc
     2041 gggcggcacc cgttcacggc gctgggatcg ctgctctccc tgaccctgct gtacctcctg
     2101 ttcgacctgt actggatcgg ggcggcgacg gtcgagcagg tgttcgcgcg ggccgtggcc
     2161 gaggtgcaga tggaagtgtt tgtccgggag gaagtggccg agggggaggt cgcgggcgtc
     2221 caggcggagg tcgccgcgat ccccggggtg accggccatc agtacatctc gaaggaggcg
     2281 gcgcgagaga tcatgcaggc gtcgctgggc atggacctgc tcgccgggta tgacaccctc
     2341 aacccgctgc cgcggtcgtt cgtgctgacg ttcgcgccgg cggcgctcac gagcgacggt
     2401 ctcgcggcga ttgccggaga gctgcggcgg atgccggcgg tggccgacct ctactacaac
     2461 gccgagtggc tgcgcaagac ggaggggacc caggccgtca ttctgcgggt cggcctggcg
     2521 ctcggaatcc a
//
LOCUS       DAOOKS010000262         2529 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_50764_length_2529_cov_2.450283, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000262 DAOOKS010000000
VERSION     DAOOKS010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50764_length_2529_cov_2.450283"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..805)
                     /locus_tag="PK112_05050"
     CDS             complement(128..805)
                     /locus_tag="PK112_05050"
                     /inference="COORDINATES: protein motif:HMM:NF014078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HPC11450.1"
                     /translation="MTRRYPTYRLGPDFRKRLALFRRRIVDQGFAEFAAEFAGLDEFI
                     CRAWAEDSPEKDLRRTPKEKYLLEALAFSFYDDLNREAFNRTGDTLIIVPDCLSLNNP
                     DCRKVEGEYGDTCQQCEPACQSYWVMELAGEYGARVAFSKRKLSNQIEHYAERLSNVG
                     VVGVACMIMLTSGLRTCAELDIPARGVLLSFTGCEHWNDVPFGSRFPMSWLREILEEK
                     RAARLSP"
     gene            complement(860..1399)
                     /locus_tag="PK112_05055"
     CDS             complement(860..1399)
                     /locus_tag="PK112_05055"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPC11451.1"
                     /translation="MRQPALTRLYAAATLVLVLGGCSGGMVTTKAVRVDGPPTAADSA
                     DSAAVLPADTDEQFRSLVSDGFMLYRQQRYPEAVAVLRRAVARHPDHWEPFYALGLIR
                     CAAEDYATAEAFLSESLDLAPPDSRIRSQIHLARARNFEAQARYGRAEQSYRAALALH
                     PESSDARDGLRRLTERPAR"
     gene            1548..1829
                     /locus_tag="PK112_05060"
     CDS             1548..1829
                     /locus_tag="PK112_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010897081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11452.1"
                     /translation="MANGTTPVFYKNPGLAAVLSFFWMGLGQIYNGQIAKGVFFIVLY
                     AVSVLMMLILIGFLTTPVLWIWGMYDAYSSAEKLNRELASRQGSGPAGY"
     gene            complement(1849..>2529)
                     /locus_tag="PK112_05065"
     CDS             complement(1849..>2529)
                     /locus_tag="PK112_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11453.1"
                     /translation="GIHGKPFWSGGRMIVPMFHPAAALHQPSLREVVKQDFAKLPEMI
                     AQAARKLRGRGVRLYQKTLPAFRLIPDDRPRGTVRVDFITCFYDSLNHLLTERDLGAA
                     FRSVHDHLNDRGWFIFDMNTAAALSDIWSGHVFADAREELAWVWKSEYQARSRTACLT
                     AATFVKRGDRWDRFDEIHYERAYSNTRIRQLLRSAGFTVRGFYDCYRFTPADRSTLRV
                     CVVARRRT"
BASE COUNT          455 a          717 c          944 g          413 t
ORIGIN      
        1 cgtcgtaatc ggcgatcgtc agtttcacca ggtgacgggt catgagactt ctccatcgtg
       61 acaggtcaac cggagcaaga taggcccgag ggcgggaagg cgaaagtgag aatgcggccg
      121 ggggcggtta cggcgacagc cgggcggccc gcttttcctc gaggatctct ctcagccagg
      181 acatgggaaa acgggagccg aaggggacgt cgttccagtg ttcgcagccg gtgaagctga
      241 ggaggacgcc gcgggcgggg atatcgagtt cggcgcaggt gcgcaggccg gaggtgagca
      301 tgatcatgca ggcgacgccg acgacgccga cgttgctcag ccgctcggcg tagtgttcga
      361 tctggttcga gagcttgcgt ttcgagaacg ccacccgggc gccgtactcc ccggccagct
      421 ccatcaccca gtacgactgg caggccggct cgcactgctg gcacgtgtcg ccgtactccc
      481 cctcgacctt ccggcagtcg gggttgttca gcgagagaca gtcgggcacg atgatgagcg
      541 tgtcgccggt ccggttgaag gcttcacggt tgaggtcgtc gtagaacgag aaggcgagag
      601 cctcgaggag gtacttctcc ttgggcgtgc ggcggaggtc cttttcggga gagtcctccg
      661 cccaggcgcg gcagatgaat tcgtcgaggc cggcgaactc ggcggcgaat tcggcgaaac
      721 cttggtcgac aatgcggcgg cgaaagaggg ccagtctttt tcggaagtcc ggcccgaggc
      781 ggtaggttgg gtaacggcgc gtcatcgcca gtcaatatac ggactgacgg cggccgggcg
      841 cgagaaatat gaggggggcc tagcgggccg ggcgctccgt cagccggcgg agaccgtcgc
      901 gggcatccga cgactccggg tgcagcgcca gcgcggcccg gtagctctgt tcggcgcggc
      961 cgtagcgggc ctgggcttcg aaattgcgcg cccgcgcaag gtgtatctgg ctgcggatgc
     1021 ggctgtcggg gggcgccagg tcgaggctct cggacagaaa cgcctcggcc gtggcgtagt
     1081 cctcggccgc gcagcggatc agaccgagag cgtagaacgg ttcccagtgg tcgggatgcc
     1141 gggccaccgc ccggcggagg accgccaccg cctccgggta gcgctgctgg cggtagagca
     1201 tgaaaccgtc gctgacgagc gaccgaaact gctcgtcggt atcggccggg aggacggcgg
     1261 cggaatcggc ggaatcggcg gcggtcggcg ggccgtcgac gcggacggcc ttggtcgtga
     1321 ccatgccgcc gctgcagccg cccaggacca acacgagagt cgcggcggcg tacaagcggg
     1381 tgagggcagg ctggcgcatt gactttgtcc tcctggggag cataggtcga aatatcggcg
     1441 cggccggcgt gacgcctgag cgggggcggg aaaaagggtt aaataaaccg ctggattgta
     1501 gataatatgg gcaggaatcc gagcggctga tcaaagagag gacaggtatg gcgaacggaa
     1561 caacacccgt gttctacaag aatccggggc tggcggcggt gctgtcgttc ttctggatgg
     1621 ggctggggca gatctacaac ggccagatcg ccaagggcgt gtttttcatc gtcctgtacg
     1681 cggtgtcggt gctcatgatg ctgatcctga tcggctttct caccaccccg gttctgtgga
     1741 tctggggaat gtacgacgcg tattcctcgg ccgagaaact caaccgggag ctggcgagcc
     1801 gtcagggaag cgggccggcc gggtactgag ggcccccgcc cgaggcggtc aggtccgcct
     1861 ccgggccacg acacacacgc gcagggtcga ccggtcggcg ggcgtgaatc ggtagcaatc
     1921 gtagaagccg cggacggtga aacccgcgga acgcaggagc tgtcgaatcc gggtgttgct
     1981 gtaggcgcgt tcgtagtgga tctcgtcgaa acggtcccag cggtcgccgc gtttgacgaa
     2041 ggtggcggcg gtgaggcagg ccgtgcggga ccgggcctgg tactcgcttt tccacaccca
     2101 ggcaagttct tcgcgggcat cggcgaagac gtggccgctc cagatgtcgg acagggcggc
     2161 agccgtgttc atgtcgaaga tgaaccagcc gcggtcgttg aggtggtcgt ggaccgaccg
     2221 aaatgccgcg ccgaggtcgc gttcggtcaa caggtggttg agcgaatcgt agaagcaggt
     2281 gatgaagtcg acgcgaaccg tgccgcgcgg gcggtcatcg gggatgaggc ggaaagcggg
     2341 gagagttttc tggtagagac ggacaccccg gccgcggagt ttgcgggccg cctgggcgat
     2401 catctcggga agcttggcga agtcctgctt gaccacctca cggagagagg gctggtgcag
     2461 ggcagccgcc ggatgaaaca tcggaacgat catccgccca cccgaccaga acggcttgcc
     2521 gtgaatgcc
//
LOCUS       DAOOKS010000263         2526 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_50849_length_2526_cov_3.046945, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000263 DAOOKS010000000
VERSION     DAOOKS010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50849_length_2526_cov_3.046945"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="PK112_05070"
     CDS             <1..709
                     /locus_tag="PK112_05070"
                     /inference="COORDINATES: protein motif:HMM:NF025930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HPC11454.1"
                     /translation="GDAVRLGLRTVRHIGETARATIESEQARGPFTSLKDFVRRSRLG
                     PQPLAQLARVGAFDCFGYHRRQALWIVLGLSRRAGELDLAVEETGHTTLRPMHIGEQI
                     VADFKGMDLSTGPHPMALIRDQLAACKILAAADLRHRRDKSRVAVAGVVVIRQRPVTA
                     KGFLFLTMEDETGFINVVVMPSMLARHRRTIVHHQALIVQGILERQDGVINVIGRQFA
                     PLEAAPGDVTFRSRDFR"
     gene            complement(1095..>2526)
                     /locus_tag="PK112_05075"
     CDS             complement(1095..>2526)
                     /locus_tag="PK112_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11455.1"
                     /translation="ESTTYIVAAVDPAGFESHFSEPVEAVHVPPRDKDILVLTHGSLA
                     GVQFQSLSRVVEFYDSLLTGYDYDLYDWGDSNRIYIETDWHLLMEYNLVVIDDDLRDG
                     IFRPVWEDTTQAFTRYLLSGGRLAYFGSGGSMAGVDAITGAPSTRNLTHSLVRRFFGA
                     ESGRIVPAIYLAALYHFPPSYADTIFGFQRADPAALEYPALTYDLASGRINPGALQLW
                     PTNAPLSVSTFAPTAAGEVTHLFGADAAINSTMAGEPVGIRRQVGPAETYLFGFHLWY
                     LELPGARALIDGMFDRAVLARFDPDTVLMVSAWSLEPETLTVHVGNLPSGLSVYDIES
                     GSVRLSETVVPLSTEYTSGVPGFVGDVLAVRVSAADLLSLYGQVYGYFRIPIKVSGSF
                     AGGDPFAVYAGLVLLGKLVGDYDWNGKVNIADITMGVDRLFRGAYPELAPEDMDVSGD
                     CRFGVADLAVLISYVFRDGPPLMRCP"
BASE COUNT          502 a          841 c          780 g          403 t
ORIGIN      
        1 aggcgacgcc gtgcggctgg gcctgcggac cgtgcggcac atcggcgaaa ccgcgcgggc
       61 gacgatcgaa agcgaacagg cccggggacc gttcacttcg ctcaaagatt tcgtgcgccg
      121 cagccgtctc ggcccgcagc cgctggcgca gttggcgcgg gtcggggcgt tcgattgttt
      181 cgggtatcac cggcggcagg cgctctggat tgtgctcggc ctttcgcggc gcgcgggaga
      241 gcttgactta gcagttgagg aaacaggcca tacgacattg cgccccatgc acataggaga
      301 gcagattgtt gccgatttca agggcatgga tctttccacc gggccgcacc cgatggcgct
      361 gatccgagac cagctggccg catgcaaaat cctcgccgcc gccgacctgc gccatcgccg
      421 cgacaaaagc cgggtcgcgg tcgccggcgt ggtcgtcatc cgccagcgcc cggtgaccgc
      481 caagggtttt ctcttcctca ccatggagga cgaaaccggg ttcatcaacg tcgtcgtcat
      541 gccgagcatg ctggcccgcc accgccgcac cattgtccac caccaggcgc tgatcgtgca
      601 agggattctt gaacgccagg acggagtgat caacgtgatc gggaggcagt tcgcgccgct
      661 cgaggcggcc ccgggggatg tcacgttccg ctcgcgcgat ttccgctaag cccgcccccc
      721 caaacagagc cacggcgccc gccatcgcga cggagcgccg tgtcacacaa acctcacgaa
      781 ctcgatcagc aagcgccgat cacttgtaca ctttgcagat caccaccggc gggcacaccg
      841 ggtagatgct caggcgcccg cacgggctcg gcttcttgcc gatcggatcg atgatataga
      901 gcatgcgacc tccctgaaag gagagacggt tgaaacctcg gaccggcaca gcgccggttc
      961 gacagccacc gcttcggcag cggcgccatt cctgacgaca atgtatcctc cggactccct
     1021 cttgacaaca agaaaaagaa cctcctttcg gcgcccccgg aacctgtgtc cggctcccgc
     1081 gcaccgggcc ccggtcaggg acaccgcatg agcggcggac cgtcgcggaa cacgtacgaa
     1141 atcagtaccg ccagatccgc tacgccgaac cggcagtcgc cggagacatc catgtcctcg
     1201 ggcgccaact ccggataagc accgcggaag aggcgatcga cccccatggt gatgtcggcg
     1261 atattcacct tgccgttcca gtcgtagtct ccgaccagct tgccgagcag cacgagcccc
     1321 gcgtacacgg cgaacggatc gccgccggcg aaggatccgg agactttgat cggaattcgg
     1381 aaatacccgt agacctgtcc gtagagcgac agcaggtctg cggcggagac cctgaccgcg
     1441 agcacgtcac ccacaaaccc cggcacaccg gacgtatact ccgttgacag agggaccacc
     1501 gtctccgaca gccggaccga tcccgactcg atgtcgtaca ccgacaggcc ggacggaagg
     1561 ttgcccacat ggacagtcag cgtctccggc tcaaggctcc aggccgatac catcagaacg
     1621 gtatccggat caaaccgcgc cagcaccgcc cggtcgaaca tcccgtcgat gagcgcccgc
     1681 gcgcccggca attccaggta ccacaggtgg aatccaaaca ggtacgtttc ggccgggccg
     1741 acctgtcgcc ggatacccac gggttctccg gccatggtcg aattgatggc ggcatcggct
     1801 ccgaacaagt gggtgacctc cccggcggcg gtcggcgcga aagtcgagac cgagagcggc
     1861 gcattagttg gccagagctg cagggcgcca gggttgatgc gcccgctcgc gagatcatag
     1921 gtcagcgcgg ggtactccag cgccgccgga tcggcccgct ggaatccgaa tatcgtgtcc
     1981 gcatagcttg gcggaaaatg gtagagggcg gccaggtaga tggccgggac gattcggccg
     2041 gactccgctc cgaaaaaccg tctcaccaac gaatgggtca ggtttcgcgt cgagggcgcg
     2101 cctgtaatgg catccacccc ggccatgctg ccccctgaac cgaaatacgc cagacgcccc
     2161 ccagaaagga ggtaccgggt gaacgcctgc gtggtgtcct cccagaccgg gcggaagatg
     2221 ccgtcccgca gatcgtcatc gatgaccacc agattatact ccatcagcag gtgccagtcg
     2281 gtttcgatgt agatgcggtt ggagtcgccc cagtcgtata ggtcgtaatc gtagccggtc
     2341 agcagtgagt catagaactc gaccaccctg ctgaggctct gaaattgcac gcccgcgagc
     2401 gaaccgtggg tgagcacgag gatatcttta tcgcgcggcg gcacgtgcac cgcttcgacc
     2461 ggctccgaga agtgcgactc gaatccggcc ggatcgactg ccgcgacgat gtaggtcgtg
     2521 ctctcc
//
LOCUS       DAOOKS010000264         2516 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_51136_length_2516_cov_3.727753, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000264 DAOOKS010000000
VERSION     DAOOKS010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51136_length_2516_cov_3.727753"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="PK112_05080"
     CDS             complement(<1..267)
                     /locus_tag="PK112_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HPC11456.1"
                     /translation="MNLEQVLDLLRSDKDISENVTHWRSFDARPARYVDFPGALDGRL
                     IGALKTRGIDRLYTHQGEAVAHVAAGRDVVVVTPTASGKTLCYNL"
     gene            complement(330..872)
                     /locus_tag="PK112_05085"
     CDS             complement(330..872)
                     /locus_tag="PK112_05085"
                     /inference="COORDINATES: protein motif:HMM:NF019610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaemetzincin"
                     /protein_id="HPC11457.1"
                     /translation="MAILRSKIVVVPLGEVDPMMVNRLAADIGPVFNRSVDILKGMKM
                     PSEALNVVRSQYYVTVLLAKLERVKANSREKVIAVCEEDLYLPDQDTLLGHADIVAGT
                     SVVSLYQLRQEFYGLPEDEKKVYPRLYKEALHQLAHLFELRECRNPRCVNYYSQMMLD
                     IDNKGRKFCDVCRRKLTGVV"
     gene            complement(954..1145)
                     /locus_tag="PK112_05090"
     CDS             complement(954..1145)
                     /locus_tag="PK112_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11458.1"
                     /translation="MNGPKDAHHRPFLGMYFKCCRVYSRIYLNREGSAFVGWCPKCAG
                     QVRVGVSPTGRRDRFFTAE"
     gene            complement(1225..1929)
                     /gene="deoC"
                     /locus_tag="PK112_05095"
     CDS             complement(1225..1929)
                     /gene="deoC"
                     /locus_tag="PK112_05095"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HPC11459.1"
                     /translation="MSDPLLHELHRRFDHAALKPDVTAADIGRLCAEARAHEFCGVAV
                     NPVWIPLCGDHLRGTGVKVIGVAAFPLGANRTDVKVEEAVKGAADGAREIDLVANIGW
                     LVEGRCADVAREISAVRRALPYNVLLKVIIEAGLLPASRWAEAAQAVIDGGAQFVKTG
                     TGFFGGATVEQVRALSRAAGGRIEVKASGGIRTLADARAMLAAGASRLGSSASVAIMR
                     ELASPEPGGDTAAGSH"
     gene            complement(1926..>2516)
                     /locus_tag="PK112_05100"
     CDS             complement(1926..>2516)
                     /locus_tag="PK112_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HPC11460.1"
                     /translation="GSPGRFVRTASRRDFSLPPGGPTLLDLMVAAGRPTYAVGKIWDL
                     FAARGITSHVKPEGNAAGMEATIELVRGDRDHGLIFTNLVDFDQAWGHRRDPANFALA
                     LEDFDRRLGALLDALREDDMLIITADHGCDPTLARHTDHTREYVPLLAYGRRVKAGVN
                     LGARATFADVGATVAELFRLPGSLAGNSFAGEVGLR"
BASE COUNT          430 a          800 c          883 g          403 t
ORIGIN      
        1 gaggttgtag cagagcgtct tgccggaggc ggtgggggtg acgacgacca cgtcgcggcc
       61 ggcggctacg tgggcgaccg cctcgccctg gtgggtgtag aggcggtcga tgccgcgggt
      121 cttgagggcc ccaatcaggc ggccgtcgag ggcgccgggg aaatcgacat agcgggccgg
      181 acgggcatcg aacgaccgcc agtgcgtcac attctcagaa atgtccttgt cggagcggag
      241 gagatcgaga acttgttcga ggttcatgga tagccagtct gggtttgggc gccggaggag
      301 ccgatcgcgg gcgccggcgg cgcggccgtt cacaccacgc cggtcagttt acggcggcac
      361 acatcacaga acttccggcc cttgttgtcg atatcgagca tcatctggct gtagtagttg
      421 acgcaccggg ggttgcggca ctcccgcaac tcgaacaaat gggccaactg gtggagcgcc
      481 tccttgtaca gccggggata caccttcttc tcatcttcgg gcaggccgta aaactcctgc
      541 cgcaactggt acagggacac caccgaggtt ccggcgacaa tgtcggcgtg gccgagcagg
      601 gtgtcctggt cgggaagata gaggtcttct tcgcacaccg cgatcacttt ttcgcgggaa
      661 ttggctttga cgcgctcgag tttggccaag aggacggtga catagtactg gctgcggacg
      721 acgttgaggg cctcgctggg cattttcatg cccttgagga tgtcgacgga ccggttgaac
      781 accgggccga tgtcggcggc taaccggttg accatcatcg ggtcaacctc accgagcggg
      841 acgaccacga tcttagatct caatatcgcc atgaacccca attcccggcc gtcggctcgg
      901 gcggccgcaa cctgtcgccg ggtcgccctt catcggcagc cggcggttgt gcgtcactcg
      961 gccgtgaaaa agcggtccct gcggcccgtc ggggacacac ccacccgcac ctggccggcg
     1021 catttcggac accacccgac aaaggccgaa ccctcccggt tcaggtagat gcgcgagtag
     1081 actcggcagc acttgaaata catcccgaga aaaggccggt gatgggcgtc tttcggaccg
     1141 ttcatacgct gacaagaata cggaagaaaa acggaaaaat caatcacttg gaggaggccc
     1201 tgggggcgaa ggattcgatc gccgtcagtg agacccggct gcggtgtcgc cgcccggttc
     1261 cgggctggcc agttcgcgca tgatcgcgac cgaggccgag ctgcccagcc gcgaggcgcc
     1321 ggcggcgagc atggcgcgcg catcggccag cgtgcggatc ccgcccgagg ctttgacctc
     1381 gatccggccg ccagctgccc gagaaagagc gcgcacctgc tccacggtcg ccccgccgaa
     1441 aaacccggtg ccggtcttga cgaattgtgc gccgccgtcg atcaccgcct gcgccgcttc
     1501 ggcccagcgg gaggcgggga gaaggccggc ctcgatgatc actttgagca ggacgttgta
     1561 ggggagggcg cggcgcacgg cgctgatctc gcgcgcgacg tccgcgcacc gtccctcgac
     1621 cagccagccg atgttggcga ccaagtcgat ctcccgcgca ccatcggccg cgcccttcac
     1681 ggcttcttca accttgacgt cggtgcggtt cgctccgagc gggaaggcgg cgaccccgat
     1741 cactttgacg cccgttcccc ggaggtgatc gccgcagagg gggatccaga cgggattgac
     1801 ggcgacgccg cagaattcgt gggcgcgcgc ctcggcgcac agccgcccga tgtcggccgc
     1861 ggtgacgtcc ggtttcagcg cggcgtgatc gaagcgacgg tggagctcgt gcaggagcgg
     1921 gtcgctcatc gcagtcccac ctctccggcg aaggagttcc cggccaggct gcccggcagg
     1981 cggaacagct cggcgaccgt ggcgccgacg tcggcgaagg tcgcgcgggc gccgagattg
     2041 acgccggctt tcacccgccg cccgtacgcc aggagcggga catactcgcg ggtgtgatcg
     2101 gtgtggcgcg ccagggtcgg gtcacagccg tggtcggccg tgatgatcag catgtcatcc
     2161 tcgcgcaggg cgtcaaggag cgcgccgagg cggcggtcga aatcctccag ggcgagggcg
     2221 aaattcgccg ggtcgcggcg atgcccccag gcctgatcga aatcgacgag gttcgtgaaa
     2281 atgaggccat ggtcgcggtc gccccggacc agctcgatgg tcgcttccat gcccgccgcg
     2341 ttgccctcgg gcttcacgtg ggaagtgatg ccccgggcgg cgaacaggtc ccagatcttg
     2401 ccgaccgcgt acgtggggcg gccggccgcg accatcaggt cgagcagggt gggtccgccg
     2461 gggggcagcg agaaatcgcg gcggctggcg gtgcggacaa accgtccggg ggagcc
//
LOCUS       DAOOKS010000265         2511 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_51272_length_2511_cov_3.517508, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000265 DAOOKS010000000
VERSION     DAOOKS010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51272_length_2511_cov_3.517508"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="PK112_05105"
     CDS             <1..279
                     /locus_tag="PK112_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11461.1"
                     /translation="VAVGITEEEKFVTKTDSSTDFASLTALARDAGLIEYFKLDSAAL
                     MKDGYAKGLLPAALSAELKKFIRRKQTVTLRVKLARAGEDPDAPSGSL"
     gene            complement(300..1652)
                     /locus_tag="PK112_05110"
     CDS             complement(300..1652)
                     /locus_tag="PK112_05110"
                     /inference="COORDINATES: protein motif:HMM:NF015211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HPC11462.1"
                     /translation="MNALATDCESIDWRRALEDPAYFAQAVLGVTLHAGQRQWLENAL
                     ARENVLVTGNRWGKSFVSAVKLLRHALFRIRGSLAFDGSGRYRAVAASITQDQANLLF
                     NQVVRLARQSPVLRPRIASLVRSPYPMLTLGNGAVIEARSTQNRGEYLLGNDYDLFVF
                     DEVAFEADPEYVVEEVLLMRLADREGMLDLVSTPNGKNWFYRRVRELAQGARPGYFQS
                     GDSRANPYISRAFLDERVRHFTARRLAQNIEGQFVDSGGEVIRGSDIDAALAGISGSE
                     EAGGAASGEIRYLSGWDLARKRTATVGITVAAADGPARVVALERLKETDWATVIARIR
                     ARQEQYPGQLVIDATGLGDPIVEALREYRPTAVIFTGPRKAELLTNVELFHARRRIVY
                     ERWELPDGPGRTWSLEDELRRARWDDNCECDSLMALALALWPLRTRPAPVIAPRLGRA
                     "
     gene            complement(1722..>2511)
                     /locus_tag="PK112_05115"
     CDS             complement(1722..>2511)
                     /locus_tag="PK112_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11463.1"
                     /translation="ARVNLTVEDGRRQFRYAADHGDLDLDRPDFYYIPLSGGVNAPLG
                     RSVLQAVPFVAYIEQQLVDDMRRSSHNAGFHRLHVKVTPPERLAGESDTAYVQRINGY
                     FDETVGMIRSCAIDENPVTWDNVAIDYIGPDATRSVTNSWFFSHRSMIEEICAGTGLA
                     PFLLGYSYGATTSWSGFKFDLVMRQVRSVQAEMRRLIEWIAAIDLALAGFSARVRWEF
                     DNTFAYQAGERAGVESTRINDLIRLYEVGLIDRATAAAKAAGLV"
BASE COUNT          414 a          866 c          843 g          388 t
ORIGIN      
        1 gtcgcggtgg ggatcacgga ggaggagaag tttgtcacca agaccgacag cagcaccgat
       61 ttcgcgagtc tgacggcgct ggcgcgcgac gccggcctga tcgagtattt caagctggac
      121 agcgcggcgc tgatgaagga cggttacgcg aagggtctgc tgcctgcggc gctcagcgcg
      181 gagctgaaga agttcatccg ccggaagcag acggtcacgc tgcgggtgaa actcgcccgg
      241 gccggggagg atccggacgc accttccggc tccctgtagg cgattgccgg tcgccggtct
      301 cacgcccgcc cgaggcgcgg ggcgatcacc ggggccgggc gcgtgcggag cggccacaat
      361 gccagggcca gcgccatgag gctgtcgcac tcgcagttgt cgtcccagcg cgccctccgc
      421 agttcatcct ccagcgacca cgtccgcccc ggaccgtcgg gcagctccca ccgttcgtac
      481 acgatccgcc gccgggcgtg aaacagctcg acgttggtga gcagttccgc tttgcgcggg
      541 ccggtgaaga tcaccgcggt cggacggtac tcgcgcaggg cctcgacgat cggatccccc
      601 agcccggtgg cgtcgatcac cagttggccg gggtactgct cctggcgcgc cctgattctc
      661 gcgatcaccg tcgcccagtc cgtctccttc agccgctcga gggcgaccac ccgggccgga
      721 ccgtccgccg ccgcgaccgt gatcccgacc gtcgccgtcc gcttgcgcgc gagatcccac
      781 ccggagagat agcggatctc cccggaggcc gccccgccgg cctcctccga cccggagatt
      841 ccggccagcg cggcatcgat atcgcttccg cggatcacct cgccgccgct gtcgacaaac
      901 tgaccttcga tattctgcgc gagccgccgc gcggtgaaat gccgcacccg ctcgtcgagg
      961 aaagctcggg agatgtaggg attggcgcga ctgtcgccgg actggaaata ccccggccgc
     1021 gccccctgtg ccagctccct cacccgccgg tagaaccagt tcttcccatt gggcgtcgag
     1081 accagatcga gcatcccctc ccggtcggcc aggcgcatga ggagtacctc ctcgacaaca
     1141 tactcagggt ccgcctcgaa cgccacttca tcaaagacaa acaggtcgta gtcgttcccg
     1201 agcaggtact cgccccggtt ctgggtggac cgggcctcga ttaccgcgcc gttgccgagg
     1261 gtcagcatgg ggtaggggga gcggacgagc gaggcgatcc gggggcgcag cacgggggac
     1321 tggcgggcca gtcggaccac ctggttgaac aggaggttgg cctggtcctg cgtgatcgac
     1381 gccgcgaccg cgcggtaccg ccccgacccg tcaaacgcca gcgagccgcg aatccggaac
     1441 agggcgtggc ggaggagttt cacggccgag acgaatgact ttccccaccg gttgccggtc
     1501 accagcacgt tctcccgggc cagcgcgttc tccagccact gccgctgtcc ggcgtggagc
     1561 gtcacgccga gcacggcctg cgcgaagtac gccggatcct cgagcgcgcg ccgccagtcg
     1621 atgctctcac agtcggtcgc aagcgcgttc atggagcgtc cttccggtaa tctcaaatcg
     1681 gccgggtgcg gccgcggggc gcgcggcgcg gccgggccgg ctcacacgag cccggcggct
     1741 ttcgccgcgg ccgtggcccg gtcgatcaat cccacctcgt acagccggat gagatcgttg
     1801 atccgggtcg attcgacccc ggcccgctcg ccggcctggt aggcgaaggt gttgtcgaat
     1861 tcccagcgca cgcgcgcgga gaagccggcc agggcgaggt cgatcgcggc gatccactcg
     1921 atcaggcggc gcatctccgc ctgcaccgag cgcacctgcc gcatgaccag gtcgaacttg
     1981 aaccccgacc acgaagtcgt ggcgccgtag gaatagccga gcaggaacgg cgcgaggccg
     2041 gtccccgcgc agatctcctc gatcatcgat cggtggctga aaaaccaact gttagtcacg
     2101 ctccgggtgg cgtccggacc gatatagtcg atggcgacgt tgtcccaggt gaccgggttc
     2161 tcgtcgatcg cgcacgaccg gatcatcccg accgtctcgt cgaagtaacc gttgatccgc
     2221 tgcacgtacg ccgtgtcgga ttcgccggcg agacgctccg ggggcgtcac cttcacatgg
     2281 aggcggtgaa accccgcgtt gtgactggag cgccgcatgt catcgaccag ctgctgttcg
     2341 atataggcga caaacggcac tgcctgcaga accgagcgcc ccagcggggc gttgacgccg
     2401 ccggagaggg ggatgtagta gaagtcggga cgatcgagat cgaggtcgcc gtggtcggcg
     2461 gcatagcgga attgccggcg gccgtcctcc accgtaagat tcacccgtgc g
//
LOCUS       DAOOKS010000266         2490 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_51874_length_2490_cov_2.916222, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000266 DAOOKS010000000
VERSION     DAOOKS010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51874_length_2490_cov_2.916222"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="PK112_05120"
     CDS             <1..206
                     /locus_tag="PK112_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11464.1"
                     /translation="TVAGVPARIVRRGRQKVDEELPATVLDHGRSVHTEIGEHNNRNG
                     RKPGIREVRTDGSAPPERAASGS"
     gene            complement(259..666)
                     /locus_tag="PK112_05125"
     CDS             complement(259..666)
                     /locus_tag="PK112_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11465.1"
                     /translation="MRPLSDWIAHASPYRLDSLFLPRQPLKPHVVLPPVVIPAGKLDF
                     FFDTACVEGNLVLRHDGRVELTDITESHPLQEFADYVRAAVAMAMCYPMRDADGVPVT
                     VTMRYRCIITHDGSPQVLTDDTSIVATQRSLDE"
     gene            complement(1441..2244)
                     /locus_tag="PK112_05130"
     CDS             complement(1441..2244)
                     /locus_tag="PK112_05130"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPC11466.1"
                     /translation="MDLLGDPTVADHPYSLPHLYDVAFSFRDYARAVDFLLAAARRAS
                     GRDPASVVELGCGPGQYCREFARRGLPAFGVDRAPEMAAYAARLAAGENLPCAILEAD
                     IRRFTLPHPVDLACCMMATFHLLLTDDDVAAHLDAVADNLNPGGIYIIELSHPRDFLT
                     RQRSTRNLWTVDRDGLSVTVDWGSDAVTDPLSEITTGTVSYAVSGPSGAARHESREQW
                     REISAGLIRALIRLSGRFAIVAWYGDLDTGVPFDAGDRAWRMILVLRLC"
     gene            2298..>2490
                     /locus_tag="PK112_05135"
     CDS             2298..>2490
                     /locus_tag="PK112_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HPC11467.1"
                     /translation="MTAKTPKKKLTYADAGVDIAAGDEAVARIKALARGTFNAQVLSE
                     IGAFGGFFRPDFQGMTAPVL"
BASE COUNT          481 a          758 c          887 g          364 t
ORIGIN      
        1 ccacggtcgc cggagtcccg gccaggattg tccggcgcgg gcggcagaaa gtggatgagg
       61 agctgccggc gacagtgctc gatcacgggc ggagcgtcca cacggagatc ggcgagcaca
      121 acaaccgcaa cgggcggaaa ccgggcatac gggaagtacg gaccgacggc tccgcgccgc
      181 cggaacgggc ggcctcggga agttaggttc ccgcaagaca cgggcaacgc gcccggcgcc
      241 cggcagcctg ctggcccatc actcgtcaag gctgcgctgg gtcgccacaa tcgatgtgtc
      301 gtcagtcagc acctgaggcg acccgtcgtg agtaatgatg caccgatagc gcatcgttac
      361 ggtgacaggc actccatcgg catctctcat cgggtagcac attgccatgg caacggccgc
      421 ccgcacatag tcggcaaact cctggagggg atgggattcc gtaatgtctg tcagctcgac
      481 ccggccgtca tgccgaagga ccaggttccc ctccacgcag gcggtgtcga agaagaagtc
      541 gagcttgccg gccgggatca ccaccggggg gaggacgaca tgcggtttga ggggctgtcg
      601 cggcaggaac agtgaatcga gccggtacgg cgaagcgtgc gcgatccagt ccgagagcgg
      661 ccgcattcca ggatacgcat cttggtcgcc caagcccagg ccgtacacaa cctcacccgt
      721 atccgcccca gagttggggg agccggcaac aaattcaggt ccatactggc cgaccctggg
      781 gacgtctgca tgttgaggtc gcgggcctga gctgtccggt gccacgcgaa ccttgccgac
      841 atacccccgg taccaatcgg catcacccgc agcacctgat tgtgcgcaag cggattggag
      901 agggggaaac cgggcggcac tcccttggcc ggcagccacc ggatcctcgg ccgtggtcgg
      961 cgacggcgct atggccagca ggagcaggat ggcggccgcc gccgcatgaa ccgaggcagc
     1021 cgcgaggagt tgaatctgat gctgtcgtcc gcggtgatcg gtaatccctc gaatcgcgct
     1081 cggtatgcga tatgtgcttt cagagcttgt ccgcataagg cacccccgtg agcttggaat
     1141 agtatagtca gcggttgcga cggcccgcaa cagtcaatct cgcgcccgcg aatttggccg
     1201 agctccttaa ggcagacgcc gcggcggagg agtcaggggg cggcagcgac aaccgcacgg
     1261 ggagtgcgga gaggacggcg gcggcctttt cgcgcataac cggccgggaa tggcgttggc
     1321 ctcgatcact cgcggctcac gcgcggaccg cctcgcggtc tcagtatcta cggcgggttc
     1381 gcgcaggaaa gcagtgtgcc ggtccggccc tcctccgata ggcaggcacg aaggcgggcg
     1441 tcaacacagg cgcaggacga ggatcattcg ccaggcgcgg tcgccggcgt cgaatgggac
     1501 accggtgtcg agatcgccat accaggcgac aatggcgaac cggccggaga gccttatgag
     1561 cgcacgaatg agaccggccg aaatctcccg ccactgctcg cgggattcat gacgcgccgc
     1621 gccggagggc ccggagacgg cgtacgaaac ggtgccggtc gtgatttcgg aaagcggatc
     1681 cgtgaccgca tcgcttcccc aatcgacggt gacggagagg ccgtcgcggt cgacggtcca
     1741 gaggttgcgg gtgcttcgct gccgagtgag aaagtcgcgc gggtggctca actcgattat
     1801 gtagatgccg cccgggttga ggttgtcggc caccgcgtcc aggtgggcgg ccacgtcgtc
     1861 gtcggtcagg agcaggtgga aggtggccat catgcagcag gccaggtcaa cgggatgggg
     1921 gagggtgaag cggcgtatgt cggcctcgag gatggcgcac gggaggttct ccccggccgc
     1981 caggcgggcg gcgtaggcag ccatctccgg cgcgcggtcg acgccgaagg ccgggaggcc
     2041 gcgacgggcg aattcgcggc agtactggcc gggaccgcag ccgagttcga caacggaagc
     2101 gggatcgcgc ccgctcgcgc ggcgggccgc cgccagcaga aaatcgactg cgcgggcata
     2161 gtcgcgaaaa ctgaaagcga catcgtagag atgcgggaga gagtaaggat gatcggccac
     2221 ggtcgggtct ccaaggaggt ccatccggga catccggcgg acgttgacat ttgcataccc
     2281 cttccgtata tagcgccatg acagcgaaga cacccaagaa aaaactgacc tacgcggacg
     2341 cgggcgtgga cattgcggcc ggggacgaag cggtggcgcg gatcaaggcg ctggcgcgcg
     2401 ggacgttcaa cgcacaggtg ctgagcgaaa tcggcgcatt cgggggattc ttccgccccg
     2461 acttccaggg aatgaccgcg ccggtgctgg
//
LOCUS       DAOOKS010000267         2487 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_51958_length_2487_cov_3.120477, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000267 DAOOKS010000000
VERSION     DAOOKS010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51958_length_2487_cov_3.120477"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="PK112_05140"
     CDS             <1..789
                     /locus_tag="PK112_05140"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HPC11468.1"
                     /translation="IIDDLVDNQKAGGRTLAVEEQCLFASMIDDWINSLRRGRPADAF
                     QEQFLETLHRYRLPLWPWEKLAEAMVYDLYHNGFATFREFLRYTEGAAVAPASVFMHL
                     CAVRPHATGILEPAFDIRRAARPLAVFSYLVHIMRDFRKDQTAHLQYFADDLMAEEGA
                     APEDLQQAARGGEIVRPVRDLMGRYRELAERYRRISRRMLDRVQPDLAPRYGLSLEII
                     YQLYLQIFERVDPRYGDWSAAELQPPPAAVQARIEATIRTFRAA"
     gene            912..2255
                     /locus_tag="PK112_05145"
     CDS             912..2255
                     /locus_tag="PK112_05145"
                     /inference="COORDINATES: protein motif:HMM:NF024985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HPC11469.1"
                     /translation="MARLVGIICVGVLTAAAAGAEEVAVTVYNSNLGVVSEVRDLSFV
                     TGTGEVAFRDVPDQIDPASVRFELVGSRQGISILEQNYAYDLVSVDKIYSRYVDYDIE
                     VLDKQGNLIGGTLLAAGQGAITLRDASGRVKVVLLENVTQVNFPTLPEGLITRPTLFW
                     RYAAETGGSFPARVSYQTYGLSWEAEYVGVLSADEKTLDLSGWASITNNTSKTFADAT
                     LTLVAGDISRAPQAKAAGMARMDMLATAEAGFEEKAFFEYHLYTLPRPSTVAGKEVKQ
                     ISLFEPARTAAEKRFVYRPEQNAQQVEVALQVVNAEQAGLGMPLPEGRVRVFQADADG
                     SLILLGEDRINHTPRDEKIDIRVGYAFDIVPEEKLMNQTRVSQQVEERAYELQLRNRK
                     DEPVTVHVEKALPGDWQVIAADFRYERKDARTLTFDVPVPARDTTTLNYTVRVKW"
BASE COUNT          460 a          703 c          902 g          422 t
ORIGIN      
        1 atcatcgacg acctggtgga caatcagaag gcgggggggc ggacgctagc ggtggaggag
       61 cagtgtttgt tcgcctcgat gatcgacgac tggatcaatt cgctgcgccg cggccggccg
      121 gccgacgcgt tccaggagca gtttctggag acgctgcacc gctaccggct gcccctgtgg
      181 ccctgggaga agctggccga ggcgatggtg tacgatctgt accacaacgg attcgcaacc
      241 ttccgggagt ttcttcgcta cacggaaggg gcggcggtag cgccggcctc ggtgtttatg
      301 cacctgtgcg cggtgcggcc gcacgcgacc gggattctcg agccggcttt tgacattcgc
      361 cgggcggcgc ggccgctggc ggtgttctcc tacctcgtgc atatcatgcg cgatttccgg
      421 aaagatcaga cggcgcacct gcagtatttc gccgacgacc tcatggcgga ggagggagcc
      481 gcccctgagg atctccagca ggcggcgcgc ggcggagaga ttgtgcgccc ggtccgggac
      541 ctgatggggc ggtaccggga gctggccgag cgttaccggc ggatctcgcg gcggatgctc
      601 gaccgggtcc agccggacct ggcgccccgc tacgggctca gtctggagat tatctaccag
      661 ctctatcttc agatattcga acgagttgac ccgcgctacg gggactggtc ggccgccgag
      721 ctgcaacccc cgccggcggc cgtgcaggcg cgcatcgagg cgaccatccg gactttccgg
      781 gccgcctgag tttgcacttt ctggggaaga aaccccgccc tacattggta gtgatgctga
      841 cggcggcgga aggatccggc gaaaggccgg cgtgaacgcc gccgggaagg tgtataacag
      901 tggaggaacg aatggctcgt ctagtcggca taatttgtgt gggcgtactg acggcggccg
      961 cagccggcgc ggaagaggtc gcggtcacgg tgtacaacag caacctcggc gtggtgagcg
     1021 aagtgcgcga cctgtcgttc gtcacgggga cgggggaagt ggcgtttcgc gacgtcccgg
     1081 atcagatcga tccagcttcg gtgcgtttcg aactggtggg gtcgcggcag ggcatttcca
     1141 ttctggaaca aaactacgcc tacgacctgg tgagtgtcga caagatctac tcgcgctacg
     1201 tggattacga catcgaagtg ctcgacaagc aggggaacct gatcggcggg acgctcctgg
     1261 cggcgggcca gggagcgatc acgctgcggg acgcctccgg gcgggtgaaa gtggttctcc
     1321 tggaaaatgt cacgcaggtg aatttcccga cactcccgga ggggctgatc acgcggccga
     1381 cgctgttctg gcggtatgcg gcggaaacgg ggggatcgtt tccggcgcgc gtgagttacc
     1441 agacctacgg gctgtcgtgg gaggcggaat atgtcggggt gctgagcgcc gacgagaaga
     1501 cgctggacct ctcgggctgg gcctcgatca ccaacaacac gagcaagacg ttcgcggacg
     1561 cgacgctgac gctggtcgcg ggggatatca gccgggcgcc gcaggcgaaa gcggccggca
     1621 tggcgcggat ggacatgctg gcgaccgcgg aagcggggtt cgaagaaaag gccttctttg
     1681 aatatcacct ctacacgctg ccgcgcccgt cgacggtggc cggcaaggag gtcaagcaga
     1741 tttctctgtt tgagccggcg cggacggcgg cggagaagcg gttcgtgtac cgtcccgaac
     1801 agaatgcgca gcaggtcgag gtggcgctcc aggtggtcaa tgccgagcag gcggggctgg
     1861 ggatgccgct gccggaggga cgggtgcggg ttttccaggc ggatgccgac ggttccctga
     1921 ttttgctcgg ggaggaccgg atcaaccaca cgccgcgcga cgagaagatc gacatccgcg
     1981 tcgggtacgc cttcgacatc gtgcccgagg agaagctcat gaaccagacg cgcgtgtcgc
     2041 agcaggtgga agagcgcgcc tacgagctcc agctgcgcaa ccggaaagat gagccggtga
     2101 ccgtgcacgt ggagaaggcg cttcccggcg actggcaggt gatcgccgcc gatttccggt
     2161 acgagaggaa ggatgcgcgg acgttgacgt ttgacgtccc ggttccggcc cgcgacacga
     2221 cgacgctgaa ttacacagtg cgggtgaagt ggtgacgcac agagcggttg acaagcccgg
     2281 tgcgagtctg tagattcgcc cagcgaatga ggaaggtctc tgaatgaacc caggaggtac
     2341 cgttttgaaa agaacagctg tgatagtcat agtgatgctc gcggccgcgg ccctgctggc
     2401 gggcagctct ttgggcgggg gcaaaccgct gaaagcgatg atcagcggcg ccaagatcgc
     2461 gatcatcgag ggccggccgg atgaagc
//
LOCUS       DAOOKS010000268         2485 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_52011_length_2485_cov_3.848148, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000268 DAOOKS010000000
VERSION     DAOOKS010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52011_length_2485_cov_3.848148"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            192..262
                     /locus_tag="PK112_05150"
     tRNA            192..262
                     /locus_tag="PK112_05150"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:224..226,aa:Gly,seq:ccc)
     gene            complement(295..774)
                     /locus_tag="PK112_05155"
     CDS             complement(295..774)
                     /locus_tag="PK112_05155"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HPC11470.1"
                     /translation="MHQSLGLRAASTIIERVVTRAGGPPLTPERVATLRERDLITCGL
                     SRPKIRYLRGLAAAVASGELRLARLARLSDEAVMEELTKVCGIGQWTAEMYLIFVLSR
                     HDVFPVLDAGIRAAVGAIYKIPPERFRAEAPLIAERWRPYRSIACRYLWEWLDSRSK"
     gene            1152..2423
                     /locus_tag="PK112_05160"
     CDS             1152..2423
                     /locus_tag="PK112_05160"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPC11471.1"
                     /translation="MRHRVIIVGGGFGGMYAAKNLRRVPVDVLLIDRRNFHLFQPLLY
                     QVATGGLSPGDITAPLRSVLKKNRRAGVIMAEVTAVDIASRIVRTSAGDFPYDTLVVA
                     AGAAHQYFGHDEWAPLAPGLKTIEDAVDIRSRLLAAFEQAEIESDEAARRALMTFVVA
                     GGGPTGVELAGAIGEIAHHTLRGEFRRIDPGQAEIILVEGAERVLQNYPPFLSEKAVR
                     SLHKLGVTVRTRTLVTDLTPESVTLASGGVTTVVPCRTVLWAAGVSASPLGRMVVGDR
                     TDLLDRVGRVETAPDLSVPGRPEIFVIGDLASYRHQTGAPLPGLAPVAMQQGRYVARV
                     IGRRLRGKTVRPFRYFDKGNLATIGRNAAVGTYKRLRFWGYPAWLTWLFVHLMYLVEF
                     DNRLVVLVQWVWSYITRNRGARLISRADGRR"
BASE COUNT          418 a          821 c          826 g          420 t
ORIGIN      
        1 ggaagccact atcaaacgat tcgtggagat ttggtacacg ccggagatcc gggccagcct
       61 gcgccggatc acgatccgcc agtaacggcc cgctcccccc gatttatgtc ggcaagggag
      121 ttgacaaccc ggcgcggccg gcatagatta gatcgccttc aggccgaagc gaccggctga
      181 acggttccaa ggcgggcgta attcagtggt agaatgcaag cttcccaagc ttgatatgtg
      241 ggttcgattc ccatcgcccg ctttttctta tggccggcgg ccggcagccc gaagtcattt
      301 cgaccggctg tccagccact cccacagata gcggcaggcg atggaacgat agggtcgcca
      361 gcgctcggca ataagcggcg cctctgcccg gaaacgctcc ggagggatct tgtagattgc
      421 gcccacggcg gcccggatgc ccgcgtccag caccgggaag acgtcgtggc ggctaagcac
      481 gaagatgagg tacatctcgg ccgtccactg gccgatgccg cagactttcg tgagttcctc
      541 catcaccgcc tcatcagaca ggcgcgccag ccgggcgagg cgcagttcgc cggaggcgac
      601 ggccgccgcg aggccccgaa ggtagcggat tttgggacgc gagagaccgc aggtgatcag
      661 gtcgcgctcc cggagcgtcg cgacccgctc gggcgtgagc ggcggcccgc cggcgcgcgt
      721 caccacccgc tcgataatcg tcgacgcggc ccggaggccc agcgactgat gcatgatcga
      781 ccgtgccagt cggtcgaacc cgcccggctg cggcgtaata tccaggggac cgatcttccg
      841 aatgacgcgt ccgagcgtcg gatccgcccc acccagatgc ttgagtatcg gtgtcgggac
      901 tagcatggcc cgaagatagg gattcaatcg gccgcgggca agtgaggaga tgtagccaaa
      961 agggaccgtc gtttgatttt cggggccgac ccggcccttg cgtggactga ctggacctcg
     1021 ttttcggccg aatccgggcc ccggcaaccg ggaaccgggc gaggcagcaa accattatag
     1081 cagtctgaag ctcccgggac cgggtccggg tgcggtccaa cctggaggct ccaagcatgg
     1141 agaaccggca catgcgccat cgagtcataa ttgtcggcgg ggggtttggg ggtatgtatg
     1201 cggcgaagaa cctccggcgc gtcccggtcg acgtgctcct gatcgatcgg cgcaacttcc
     1261 atctctttca accgcttctg taccaggtcg ctaccggcgg gctgtccccc ggcgatatca
     1321 ctgctccgct gcgttccgtg ctcaagaaga accgccgggc cggcgtcatc atggccgaag
     1381 tgaccgctgt cgacatcgcc tcccggattg tgcggacctc ggcgggcgac tttccgtacg
     1441 acaccctggt ggtggccgcg ggcgcggctc accagtattt cgggcacgac gagtgggcgc
     1501 ccctggcacc gggattgaag acaatcgaag acgccgtgga catccgcagc cgcctgctgg
     1561 cggcgttcga gcaggccgag atcgaaagcg atgaggcggc ccgccgggcg ctcatgacct
     1621 tcgtggtcgc cgggggcgga ccgaccggcg ttgaactcgc cggggccatc ggggaaatcg
     1681 cccaccatac cctgcgcgga gaattccgcc gcatcgaccc cggccaggct gagattatcc
     1741 tggtcgaagg cgccgaacgg gtcctccaga attacccgcc ctttctctct gagaaggcgg
     1801 ttcggtcgct tcacaagctc ggggtgactg tgcgcacgcg gactctggtc acggacctca
     1861 ctcccgagtc cgtcaccctg gcctccggcg gcgtcacgac cgtggtgccc tgccgcaccg
     1921 tcctgtgggc agccggggtg tcggccagtc cgctgggccg gatggtggtg ggggatcgga
     1981 cggacctcct cgaccgggtc ggacgcgtgg aaacggcccc cgacttgagc gtgcccggtc
     2041 gtcccgagat ctttgtgatc ggcgatctgg cgagctaccg gcaccagacc ggcgcaccgc
     2101 tgcccggcct cgctccggtc gcaatgcaac aggggcgcta tgtcgcccgg gtcatcggac
     2161 ggcgcctcag gggaaagacg gtgcggccgt tccgctactt cgacaaaggg aacctggcga
     2221 ccatcggccg caacgccgcg gtcggcacct acaaacgcct ccgtttctgg ggttaccccg
     2281 cctggctgac ctggttgttc gtccacctga tgtacctggt ggaattcgac aaccgcctgg
     2341 tcgttctcgt gcagtgggtc tggagctaca tcacccgcaa ccgcggcgcg cggctgatca
     2401 gccgggccga cggccggcgt tgacttcggc cccccgattt cgttccttag gtcgctcccg
     2461 gggagaagcg gcgcgcccga ccggc
//
LOCUS       DAOOKS010000269         2484 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_52038_length_2484_cov_4.372993, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000269 DAOOKS010000000
VERSION     DAOOKS010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52038_length_2484_cov_4.372993"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1821
                     /locus_tag="PK112_05165"
     CDS             <1..1821
                     /locus_tag="PK112_05165"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPC11472.1"
                     /translation="AAPLSLASGLDGAAGLDVRTQGGFGALATVSNWGAFNRHLLLLY
                     NGRPVKDYSLGGFNLAEFSPSELSRIEIVKGPQSAFYGSDAVGGVVNLIAPTALADRL
                     EAHTTVGSFGARGVAARAARRLGALGIGAWTDYTAADNRRPNSGVERLIAGARADLLS
                     GAHRLALAARYFRDSLGAPGPVPDPSFIPVYGDPESSSLTDRQEDEHYSVDLQYSLVD
                     ERAGETQIGLFWEKKKLTYRSLYNYLLFSATTDSVDVRTETVYDKRSAGANLRHRHNF
                     GPLAAAGGVDWLSGSLQYANAERSEAAAIDGPGAPAVSSYDTFASYDAGQDQVDLWGN
                     AQMGRAGRVHVDLGGRLQLVAGRRPQPSYNLGLVAEPGDALRLKLAYAYAFRLPSLAE
                     QFADDLYTAGNADLSPETARTLAATMALEPAALPVRLSLTWFRQEIDSLIQYVWDPAS
                     YRSVPQNFLRFISQGLDATCTIALRRGLSWRGSAVYQAAEQTAADGRTMREAPYVPEW
                     KWRVGMTWRSGRFDAAARLDGTSERVLYLYGGAPKTIDPVVEAAAELGVRLSAMLRMH
                     ISGEDLTDRRRPDQFGFTAGDGDYPGLGRRFFVELALAMR"
     gene            2033..2455
                     /locus_tag="PK112_05170"
     CDS             2033..2455
                     /locus_tag="PK112_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11473.1"
                     /translation="MSGWSFEYDIEVDEANRIVYEKIFGVWRIKTAESYRADFEEEVK
                     PIIDRPWAKLIDLSNWKTSYPEVIDIVGKHLAWCRRHNMQWSVNVINNTITYNQLQRM
                     FDKGATKEISRTFRNYAEAVQFLQEQGYTVRPTPPSRT"
BASE COUNT          424 a          848 c          803 g          409 t
ORIGIN      
        1 gcggcgcccc tttcgctggc ttcgggactc gacggcgccg ccgggctgga tgtgcgcacc
       61 cagggggggt tcggggcgct cgcgaccgtc tccaactggg gcgctttcaa ccgccacctg
      121 ctgctgctgt acaacggccg gccggtcaaa gactactcgc tcggagggtt caacctcgcc
      181 gagttcagcc cgagcgagct ctcccgcatc gaaattgtca aaggcccgca gtcggcgttc
      241 tacggttccg atgccgtcgg cggcgtggtc aacctgatcg cgccgaccgc gctcgccgac
      301 cgcctcgagg cgcacacgac cgtcgggtcg ttcggcgccc gcggcgttgc ggcccgtgcc
      361 gcgcggcggc tgggcgcgct cgggatcggc gcctggaccg actacaccgc cgccgacaac
      421 cgccgcccca actccggcgt cgaacggctc atcgccggtg cgcgcgccga cttactctcc
      481 ggagcgcacc gcctggccct cgcggcacgc tacttccgcg actccctcgg cgcccccggc
      541 cccgtcccgg acccgtcatt catccccgtc tatggcgatc ccgaatcctc cagcctcacc
      601 gaccggcagg aggacgagca ctattcggtg gatctgcagt acagcctcgt cgacgagcgg
      661 gccggcgaaa cgcagatcgg gctgttttgg gagaaaaaga agctcaccta ccggtcactc
      721 tacaactacc tgttgttctc tgcgaccacc gattcggtgg acgtgcggac cgaaaccgtc
      781 tacgacaagc gctcggccgg ggcgaatctc cggcaccggc acaatttcgg cccgctcgcg
      841 gcggccggag gggtcgactg gctgagcggt tcgctgcagt acgccaacgc cgagcgcagc
      901 gaggccgcgg ctatcgacgg cccgggggct cccgcggtct ccagctacga cacgttcgcc
      961 tcgtacgacg ccggccagga ccaggtggat ctgtggggga acgcgcagat gggccgggcc
     1021 gggcgcgtcc atgtcgacct cggcggccgg ctccaattgg tagcggggcg gcggccgcag
     1081 ccctcgtaca acctcgggct ggtggccgag ccgggtgacg cgctgcggct caagctcgct
     1141 tacgcgtatg ctttccgcct cccctcgctg gccgagcagt tcgccgatga cctctacacg
     1201 gccgggaatg ccgatctgag cccggagacg gcccgcacgc tggccgcgac gatggcgctc
     1261 gagcccgccg ctctccccgt tcgcctctct ctgacctggt ttcgccagga gatcgactcc
     1321 ctcatccagt acgtgtggga tccggcgagc taccgctcgg ttccgcagaa tttcctccgg
     1381 ttcatatcgc agggactcga tgcgacctgc acgatcgcgc tccgacgcgg tctgtcgtgg
     1441 cgcggctcgg ccgtgtacca ggcggccgag cagacggctg ccgacggccg gaccatgcgc
     1501 gaggcgccgt atgtccccga atggaaatgg cgggttggga tgacctggcg cagcggacgt
     1561 ttcgacgcgg cggcgcgcct cgacggcacc tcggaacggg tgctgtacct ctatggcggc
     1621 gccccgaaga cgattgatcc ggtggttgag gcggccgcgg aactcggggt tcgcctgagt
     1681 gcgatgctgc ggatgcatat ctccggagag gatctgaccg accggcgccg ccccgaccag
     1741 tttgggttca ccgcggggga cggcgactac cccggactcg gacggcgttt ttttgttgaa
     1801 ctggcgctgg caatgaggta aactgagacc aactcggttc tctcagcagc actgatttgt
     1861 tgagaacttt caggggccct tgaggaggcg accaaacgtc gggcggaacc ctatccggcg
     1921 gcgcggcgtt taagatgcga gcgagtatca cccgtcccgg ctccgggcgc ggcacggcag
     1981 tatccgaaat cgcagagagt gtggctccaa aaaggaaggg atccggttta ccatgagcgg
     2041 gtggagtttc gaatacgata tcgaggtcga cgaggccaat cgcatcgtct acgaaaaaat
     2101 cttcggggtc tggcgcatca agaccgccga atcctaccgg gcggattttg aggaagaagt
     2161 caaaccgatt attgaccgcc cctgggccaa actgatcgac ctctccaact ggaaaaccag
     2221 ctaccccgag gtgatcgaca ttgtcggcaa acacctcgcc tggtgccggc gccacaacat
     2281 gcagtggtcg gtgaacgtca tcaataacac catcacctac aaccagctcc agcgcatgtt
     2341 cgataagggg gcgaccaagg aaatctcccg caccttccgc aactacgccg aggccgtgca
     2401 gttcctccaa gagcaggggt acaccgtccg ccccaccccg ccgagccgca cgtagatccg
     2461 ccggagaggc gcgccgagag ggac
//
LOCUS       DAOOKS010000270         2467 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_52568_length_2467_cov_2.880182, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000270 DAOOKS010000000
VERSION     DAOOKS010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52568_length_2467_cov_2.880182"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(474..1262)
                     /locus_tag="PK112_05175"
     CDS             complement(474..1262)
                     /locus_tag="PK112_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11474.1"
                     /translation="MKLLMRLILLSLSLVCSGGSAGMDDVKWDVDQDTVMSDEQPLPE
                     PVLLIERFQLYVYMECEPDHEVLRNTGRMGIDIRTAYRTVGFDAITTDSSLTLRLRTL
                     NRGKREDRVSLPAESFVLYVTLRSDTASYWVERTDTGFSITSRRQSNWLELGDVRTLE
                     ANVRWMQFARDATQGDVKEFVKQLRENFADIVTLVQIAPGYYPCLPAPVVQSSTIGRR
                     LRANKTAAGYLVGFQVRGEPVDVQRVRNWVMQQQTQELGRSGKN"
     gene            1851..2453
                     /gene="rdgB"
                     /locus_tag="PK112_05180"
     CDS             1851..2453
                     /gene="rdgB"
                     /locus_tag="PK112_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HPC11475.1"
                     /translation="MQLVLATNNRDKIREIRHLLEDLPITILTAGDFLDFPEIDETGE
                     TLEANALLKARGIAAFTDLPALADDSGLEVDALGGQPGVYSSRYAGDNVTYDDNNRKL
                     LEELAGVPRAERTARFRCVIAIDWADGTHETVEGVAEGWIAEDIAPGNGFGYDPVFVH
                     SETGQRFSAMRLPEKSAVSHRGQALRRAREAISARLKARS"
BASE COUNT          470 a          745 c          801 g          451 t
ORIGIN      
        1 aatttcattc cgccgaccga ctaccgctac cccttcctga aggagtagcg ggagcgggac
       61 gcggagatcg tgccggctgc cgcaggggcc gcccgtcatc gccgttcggg ggcggcgctc
      121 ggcggggtct cccggctccc gtggagcctc tgacatcgac ccgcggcggg agcgtcgatg
      181 cggtaaaggg aactcggtcc gcctggaccg tgggggcgcc tttgccgaga tttctgctgc
      241 ttcggcaccg gaacgaaaga tatcccggtc tgcggcagga ccgttatcct atggcagggg
      301 cggcagggtt gcgggcggct cggaattgcg ccggcgcgga gtcggcggta acgtcccccg
      361 ccgttgccgc ggcgcatgtc tttcctcccc aaagtataag ccgaataggc tgttactccg
      421 acgtacatca ccggagccac ggtcgataat cgcttctgcg atctgcctcg gattcaattt
      481 tttccactgc gcccgagttc ctgtgtctgc tgctgcatca cccagttgcg cacccgctga
      541 acgtcgaccg gctcgccgcg tacctgaaaa ccgacgagat acccggcggc ggtcttgttt
      601 gcgcgcagcc gccttccgat cgtcgaagac tgcacaacgg gcgccggcag gcaggggtag
      661 taccctggcg caatctggac aagggtcaca atgtccgcga aattctcgcg cagctgcttg
      721 acaaattcct tcacgtcgcc ctgagtcgca tctctcgcaa attgcatcca tcggacgttc
      781 gcttcgagtg tgcgcacatc accaagctcc agccagtttg actgccgacg gctcgtaatg
      841 ctgaagccgg tgtccgtacg ttcgacccag tacgaggcgg tatccgatcg gagtgtgacg
      901 tataacacaa atgactctgc cggcagagaa acccggtctt cccgctttcc gcgattgagc
      961 gtgcgcagac gcagcgtgag cgacgaatcg gtggtgatgg catcaaaccc aactgttcgg
     1021 tatgcggtac ggatgtcgat tcccatccga ccggtgtttc tcagcacctc atgatccggc
     1081 tcacattcca tgtagacata caactgaaaa cgctcgatga gaagcaccgg ctcaggcaga
     1141 ggttgttcgt ctgacataac cgtgtcctgg tccacgtccc atttaacatc gtccatgccg
     1201 gccgacccac cagagcagac cagcgacaga ctcaacagga tcagccgcat caaaagtttc
     1261 atgacggctc cgttcatctc ctcgcatatc aattggccga attcgcttcc tctacaacga
     1321 cgcgacctcc caccaagcgg gcaatgtcgc gggataggtg gtgctgccgc ggcctcagcc
     1381 gcgttgtctg catgggttct gacatcaact tgcgtgccgg accgcgagct taccgacatc
     1441 tttctgggca tcgcattgac ggggaagcag ttggcgagga agacggagag cgcgcacgag
     1501 cagccgcatc ggggtttgcg cgccgttgtg acgcccacgg tggcgcagcg tgacgcatgg
     1561 tgggcgaagc cgttgggagt aaaggcatta ataaggcagt atctgcgtcg ggagtgcgtc
     1621 gggcgactcg cccgtgacgc aggcaggggg atctgcgatg gctgatcgct gcaggaatca
     1681 gaccggcgcc gggaatctcg tgcatgcacg tcgaattccg cactccggcg ttggacggcc
     1741 gacctccctc agtcgccggc agacccaggg gcgcgcttta ggcttgtgcg ccggcggatg
     1801 gatgctacat tgggggcgat tcaggacgcc cgaaagaacg gaatgacccc atgcaactcg
     1861 tgctggccac caacaaccgc gacaaaatac gggaaatccg acaccttctt gaggatctcc
     1921 cgatcaccat cctcacggcc ggtgattttc tcgattttcc ggaaatcgac gaaaccgggg
     1981 agacgctcga ggccaacgcc ctgctgaaag cgcgggggat cgccgctttc acggaccttc
     2041 cggccctggc ggacgattcg gggctggaag tggatgcgct cggcgggcag ccgggtgtgt
     2101 actcctcgcg gtacgccggg gacaacgtca cctacgacga caataaccgg aagctgctgg
     2161 aggagttggc gggggtgccg cgagcggagc ggacggcgcg gtttcgctgc gtgattgcga
     2221 tcgactgggc cgacgggacg cacgagacgg tcgagggggt ggccgagggg tggattgccg
     2281 aggatatcgc gccgggaaac gggttcggat acgacccggt gtttgtgcac agcgaaaccg
     2341 gacagcggtt ttcggccatg aggctgccgg agaagagcgc ggtgtcgcac cggggccagg
     2401 cgctgcggcg ggcgcgcgag gcaatcagcg cgcggctgaa agcgagaagc tgaggggaaa
     2461 cccgggc
//
LOCUS       DAOOKS010000271         2455 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_52970_length_2455_cov_2.769583, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000271 DAOOKS010000000
VERSION     DAOOKS010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52970_length_2455_cov_2.769583"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="PK112_05185"
     CDS             complement(<1..211)
                     /locus_tag="PK112_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11476.1"
                     /translation="MNESAVYVSPSTARSLWQEYRIYERHVEFDTHIGTVTIPFDNIE
                     SVEVQGSDVKALLRGDLRLKGFKPAI"
     gene            482..1582
                     /locus_tag="PK112_05190"
     CDS             482..1582
                     /locus_tag="PK112_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HPC11477.1"
                     /translation="MDPRTEKLAQVLVHYSLRLKKGELLKIQGEYVTLPLMTAVFEEA
                     VRIGAIPYVRVIIPQHDEIFLKKGTDAQFAYISPIARTEVNTMDVFLHIWGTANTRHL
                     SGVDPKRQALQRKCQRPLSDKLFKRMETKSLRWCGTLFPAQAEAQEASMSLAEWENFV
                     YRAGHVTSGDPVKHWTKVKKEQDRLVKILNRTDRLHIRSADTDLKMRVKGRKWINCAG
                     TENFPDGEVFTCPIEDSAEGVIRFSFPAIYVGREVEDVRLELKKGKVVKESAAKNQAY
                     LTGMLNMDKGARYVGEIAIGTNYDIDRFSKDILFDEKIGGTLHMALGRAFAEAGGKNV
                     SALHWDMICDMRKDAEVLADGKVIYRNGKFTI"
     gene            complement(1673..>2455)
                     /locus_tag="PK112_05195"
     CDS             complement(1673..>2455)
                     /locus_tag="PK112_05195"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPC11478.1"
                     /translation="RFGDGDSATVQNPAHTYSNPGAYDVSLTVETSEFGQLTTSRPHF
                     IIILGDTAHYGSDSTFAGSDLVVSINVTNSQPLARLVVPLTFGSAAWLTFDSVTRGDR
                     TAYFERLNPLNSNDLTREYVYELLADFGGGSPPLAPGSGEVLKLWFSTDELALGGQSI
                     LIDSIASPYVISFEAGYLTYSPTVAAGTGALIDVMRGDVNYDHDLSIADVTHLVAHFY
                     RGGPPPVCLQAGDVNRDFMMSVADLTYYVAYLFRGGPPPPTP"
BASE COUNT          492 a          715 c          857 g          391 t
ORIGIN      
        1 tgatcgccgg tttgaaccct ttcagcctga ggtcgcctct caggagcgcc ttgacatccg
       61 acccctgcac ctcgacgctc tcgatgttgt cgaacgggat ggtgacggtg ccgatgtggg
      121 tgtcgaactc gacatggcgt tcgtaaatgc ggtactcctg ccagaggctg cgggcggtcg
      181 acggcgagac atagacggcg gattcgttca tggacacacc tcctgtctgg ggcgccccgg
      241 cgcactgcct gcgcacacgt tcgtcgccgg gcgcgggccc cggattcccg gtctccgctc
      301 actcctacga tagaaccggg gaaaggattc ggtcaagggg aatatcagcc gcggcccgcc
      361 ggcggcgggg cccggaaagt gacggcggga gctttacaaa ccggacgcgg aggccgttat
      421 tgccctgcgc gacccagact gaaggtactg gcgatacaac cactttgacc aggagcgacg
      481 tatggacccg cggaccgaga aacttgccca ggtgctcgtg cactattcgc tgcggctcaa
      541 gaagggggaa ctgctcaaga tccaggggga gtatgtgacg ctgccgctga tgacggccgt
      601 gtttgaggag gccgtgcgca tcggggccat tccctatgtg cgggtcatta tcccgcagca
      661 cgacgagatc ttcctgaaga agggaaccga cgcccagttt gcgtacatct ctccgatcgc
      721 acggaccgag gtgaacacga tggatgtgtt tctgcacatc tgggggacgg ccaacacgcg
      781 ccacctgtcg ggggtcgatc ccaaacggca ggcgctgcag cggaagtgcc agcggccgct
      841 gtcggacaag ctgttcaagc ggatggagac caagtcgctg cgctggtgcg gcacgctctt
      901 cccggcgcag gccgaggcgc aggaagcgag catgtcgctc gccgagtggg aaaacttcgt
      961 ctaccgggcc ggccacgtca ccagcggcga cccggtgaaa cactggacca aagtcaaaaa
     1021 ggagcaggac cggctggtga aaatcctcaa ccggacggac cgcctgcata tccggtccgc
     1081 cgacaccgac ctgaagatgc gggtcaaggg gcggaaatgg atcaactgcg cggggacgga
     1141 gaatttcccg gacggcgagg tgttcacctg cccgattgaa gattcggccg aaggcgtcat
     1201 ccggttctcc ttcccggcca tttatgtcgg gcgcgaggtc gaggacgtgc gcctggagct
     1261 gaagaagggg aaggtggtga aagagtcggc ggcgaagaac caggcgtacc tcacgggcat
     1321 gctcaacatg gacaaggggg cacggtatgt cggggagatc gcgatcggca ccaactacga
     1381 catcgaccgc ttctccaaag acatcctgtt cgacgagaag atcggcggga cgctccacat
     1441 ggctctcggc cgggccttcg ccgaggcggg aggaaaaaac gtcagcgcac tccactggga
     1501 catgatctgc gacatgcgca aggacgccga ggttctggcc gacgggaaag tgatctaccg
     1561 gaatgggaag ttcacgatct agcgcgggag gcgcatccgc aaaaaaaccg gccggtgtct
     1621 gaaaagacac cggccgcggc atttgtggtt gcgagcgagc gggcaggcgc cgtcacggcg
     1681 tcggcggggg cggcccgccc cggaagagat aggcgacata gtaggtgagg tccgccacgc
     1741 tcatcatgaa gtcgcggttg acatcccccg cctgaaggca gaccggcggc ggcccgcccc
     1801 ggtagaaatg ggcgaccagg tgcgtgacgt ccgcaatgct gagatcatgg tcatagttga
     1861 catccccccg catgacgtcg atgagggcgc cggtgccggc ggcgaccgtc ggggagtagg
     1921 tcaggtaacc ggcctcgaac gaaatgacgt agggggaggc gatgctgtcg atcaggatgc
     1981 tctgcccgcc cagggcgagc tcgtcggtcg agaaccagag cttgagcacc tcgcccgacc
     2041 ccggcgcgag cggcggcgag cccccgccga agtcggcgag gagttcgtac acgtactcgc
     2101 gcgtgagatc gttcgaattc agcggattca aacgctcgaa ataggccgtg cggtcgcccc
     2161 gcgtgaccga gtcgaaagtg agccaggcgg cgctgccgaa agtgaggggg acgaccaggc
     2221 gcgcaagcgg ttgggagttg gtcacgttga tcgaaaccac caggtcgctc ccggcgaagg
     2281 tcgagtcgga accgtagtgg gccgtgtcgc caaggatgat gatgaagtgg gggcggctgg
     2341 tcgtgagttg gccgaactcc gacgtctcca ccgtgaggga gacgtcgtag gcgccggggt
     2401 tgctgtaggt gtgcgcgggg ttctgcacgg tcgccgagtc gccgtcgcca aaacg
//
LOCUS       DAOOKS010000272         2454 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_53001_length_2454_cov_2.802001, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000272 DAOOKS010000000
VERSION     DAOOKS010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53001_length_2454_cov_2.802001"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="PK112_05200"
     CDS             <1..253
                     /locus_tag="PK112_05200"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPC11479.1"
                     /translation="RVAVALGDEPGALVITVADTGPGFARDLLERGLEPRVSTKKGGS
                     GLGLVICQRIVHDHGGAIDLQNRADGGALVTLRLPVEHG"
     gene            246..1565
                     /locus_tag="PK112_05205"
     CDS             246..1565
                     /locus_tag="PK112_05205"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPC11480.1"
                     /translation="MAKILIVDDEESIRSSLKAALERRGHEVVTAESYARGETFMSGR
                     FDLIFLDVLLGDGNGIDLLKAALEKNPRHIIVMFSGHADIDMAVRATRAGAYDFIEKP
                     LSLDRVLVTVDNAVRTRRLQAEADRLALRAYGEFVGESPEILKLKSDILRAAPKAARF
                     LILGENGTGKELVANMIHRASGRSNGPFVAVNCAALPSELVESELFGHVRGAFTGARA
                     DRRGRFVEADQGSIFLDEISEMPAGAQAKILRVIETREVTPVGASRTYSVDCTIIAAS
                     NRDLETMAADGSLRQDLLYRLNVVTFHLPPLRERTQDIPLLADHFLARFAGETGSVPK
                     RLERDSLKLLCAYSFPGNIRELKNLMERVNIYCERSVVTVADLKPLLPAAARGGRRSL
                     KEAMAEFEREYLEAAIARHGGNMTEAARELGIERSLLYKKLRRLEEG"
     gene            complement(1670..>2454)
                     /locus_tag="PK112_05210"
     CDS             complement(1670..>2454)
                     /locus_tag="PK112_05210"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HPC11481.1"
                     /translation="GESFAVCRFLTAPPAYREVSLDIKPGSCPNPLNLVGPSDNGKAV
                     LPAAVLGAPDFDVRTINPPTLALGGAEVVRWSYEDVGRPKADPYDTCACHEYGPDGYM
                     DLTLKFYRAEVAPAVSFAADGDYAPLVLTGSLLDGTPLSGVDCVRVQRGGRPEGNSGG
                     GPTGKVQAPADFALANHPNPFNPSTQITFALPQAGPVTLRVYDVQGRLVRTLANGPFD
                     AGVHSVEWNGTDGTGSAVASGVYLYRLSAGETVVTRKMILMK"
BASE COUNT          416 a          825 c          816 g          397 t
ORIGIN      
        1 ccgggtggcg gttgcgctgg gggacgagcc cggggcgctc gtgattacgg tagccgatac
       61 cggccccggt ttcgcccgcg acctgctcga gcgcggtctg gaaccgcggg tttcaacgaa
      121 gaagggcggc agcgggctcg ggctggtcat ttgccagcga attgtccacg accacggcgg
      181 cgcgatcgat ctgcagaacc gcgccgacgg cggcgcactg gtcaccctca gactgccggt
      241 ggaacatggc taagatcctg attgtcgacg acgaggagag catccgcagt tcgctgaagg
      301 cggcgctcga gcgccgcggc cacgaggttg tcacggccga atcgtacgcg cgcggcgaga
      361 ctttcatgtc cggacgtttc gatctcattt ttctcgacgt gctgctcggc gacggcaacg
      421 gcatcgacct gctgaaggcg gcgctcgaga agaatccccg gcacatcatc gtgatgttct
      481 ccggccacgc cgacatcgac atggcggtcc gggccacgcg cgccggggcc tacgatttca
      541 tcgagaaacc cctgtctctg gaccgcgtgc tcgtcacggt cgacaatgcg gtgcggaccc
      601 ggcggctaca ggccgaggcc gaccgcctcg ccctgcgcgc ctacggggag ttcgtcggtg
      661 agtcgccgga gatactcaaa ctgaagtccg acattttgcg ggcggccccg aaagcggcgc
      721 gctttctcat cctcggcgag aacggcaccg gcaaggagct tgtcgccaac atgattcatc
      781 gcgccagcgg ccggagcaac ggaccgtttg tcgccgtcaa ctgcgccgct ctgccatcgg
      841 aattggtcga gtccgagctt ttcgggcatg tgcggggcgc tttcaccggc gcccgcgccg
      901 accgccgggg aagattcgtc gaggccgacc aggggtcgat tttcctcgac gagatcagcg
      961 aaatgccggc cggcgcccag gcgaaaattc tccgcgtcat cgagacgcgc gaagtgaccc
     1021 cggtcggggc aagcagaacg tactcggtcg actgcacgat catcgccgcc tccaaccgcg
     1081 acctggagac gatggcggcc gacggctcgc tgcggcagga tttgctctat cgcctgaacg
     1141 tcgtgacttt ccacctcccc cccctgcggg agcggaccca ggacatccct ttgctcgccg
     1201 atcacttcct ggcccgcttt gccggcgaaa ccggctcggt cccgaaacgt ctcgagcgcg
     1261 acagcctgaa gctgctgtgc gcttactcct tcccaggcaa catcagagaa ctcaagaacc
     1321 tgatggagcg ggtcaacatc tactgtgagc ggagtgtcgt gactgtggcc gacctgaagc
     1381 cgctcctgcc ggcggccgcg cgcggcggtc gccggtcgct gaaagaggcg atggcggagt
     1441 tcgagcgcga gtatctcgag gccgcaatcg cgcgccacgg cggcaacatg accgaggccg
     1501 cccgcgagct cggcatcgaa cgctccctgc tgtacaagaa gctgcgccgc ctggaggagg
     1561 gctaaccgcc tccgccctcg ggcgcggcgc gccgcctggc cgccccgccc gcaaaaaaaa
     1621 gcgcggccct gccggaccgc gcccttcact ttgtccctgc cgacccgacc tacttcatca
     1681 agatcatctt tcgggtcaca accgtctccc ccgccgacaa ccggtagagg tacacgccgc
     1741 tggccacggc actgccggtg ccgtcggttc cgttccactc gaccgagtga acgccggcat
     1801 cgaaaggccc gttggccagc gtccgcacga gccgcccctg gacatcgtac acgcgcaggg
     1861 tgaccggccc ggcctgggga agggcgaagg tgatctgggt tgacgggttg aagggattgg
     1921 gatggttggc gagtgcgaaa tcggccgggg cctgcacctt tccggtcggg ccgcccccgc
     1981 tattgccctc cggcctcccg ccgcgctgca cgcgcacaca atcgacgccg ctaagaggag
     2041 tgccgtcaag cagcgatccg gtcaggacga gcggtgcgta atcgccatcg gcggcaaacg
     2101 agacggccgg ggcgacctct gcgcgataga acttgagggt cagatccatg tacccgtcgg
     2161 ggccgtactc gtggcacgcg caggtgtcgt atggatcggc tttcggccgg ccgacatctt
     2221 cgtaactcca gcggacaact tcggcgccgc ccagcgcgag cgtcggcggg ttgatcgtgc
     2281 ggacgtcgaa atccggcgcg ccgagaacgg cggccggcag cacggccttg ccgttgtccg
     2341 acggtccgac gagattgagc gggttcggac acgacccggg tttgatgtcg agggaaacct
     2401 cccggtatgc cggcggggcc gtgaggaatc ggcagacggc gaagctctcc ccgt
//
LOCUS       DAOOKS010000273         2441 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_53398_length_2441_cov_4.205784, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000273 DAOOKS010000000
VERSION     DAOOKS010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53398_length_2441_cov_4.205784"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="PK112_05215"
     CDS             <1..544
                     /locus_tag="PK112_05215"
                     /inference="COORDINATES: protein motif:HMM:NF015962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0050532 - 2-phosphosulfolactate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HPC11482.1"
                     /translation="DNVRIENFKLGNSPAEFTPETVGGKFVIMTATNGTGVYRDAVGA
                     DLVLCCALVNVSAVARKAAAAGHGLVLVCSGQEGGFSIEDTICAGMLVHLLATEHKLN
                     LALNDAGSLALLLYRSNKSALKKTIQQGEHGRRLTELGFGADVETAAAVDTMPVLPVM
                     VDGRLVADVDPPAAPPAEPA"
     gene            581..>2441
                     /locus_tag="PK112_05220"
     CDS             581..>2441
                     /locus_tag="PK112_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11483.1"
                     /translation="MRHLIILAALVSLEAGAPAAAADAVRFALDVHQQDSAAGAVLLY
                     SDTATVVRGVAAAGFALAFSAEVEVTAADTAHAQFLVHAVTLGPSARTYARSYSAEYG
                     LPARIEGIEGKGGGRLMLTVTPLAFTDFDTAQCLFDHRVTGAYKIQPSASLDVHYVPN
                     SFGDYYIEAFKGVLEHHYRQFQEVFQFSLPGKFSIFLLPCASPAVLWDTRYGQVTDPT
                     RSTAFAIAANGQNTVDPFIATHTALLRSWGYAPPFLSEGAANYTSFALFDMKKLAAEG
                     RVPPLDSLLDTYAYLTAAPLVADRASATFAQYLIDSYGLEAFRALYVSADDLNLRRRL
                     ALAYGKGIGALEAEWRAYVDTSTIRFPALAQEADKAEALFQYGRMRDYARAMLELAPG
                     RIDSAFAWSRLKQAEFMLGNYYGAADAAEAQVQLDGERAVNWMTLGSFQMMNGMYAEA
                     RASLQNGQSKDPSDQTIAFNRALAALYTSDTATAESLLVEIITRPESRAPQGEARVVL
                     GEILLSRGDKRGRDRAATYFVEAMQTFQQQLSVNRTMPTAYLWLGAAALGMGDTELAR
                     DALETGLFLESRPFYLGQMNLWLGKTADVLGDHGLAQEYYGQVLAGSSAAYAQQ"
BASE COUNT          392 a          797 c          860 g          392 t
ORIGIN      
        1 ggacaacgtg cggatcgaga atttcaagct cggcaattcg cccgcggagt tcacgccgga
       61 gacggtcggc ggcaaattcg tgatcatgac ggcgacgaac gggacgggcg tgtaccggga
      121 cgcggtggga gcggacctgg tgctctgctg cgcgctggtc aatgtcagcg ccgtggcccg
      181 caaggcggcc gcggccgggc acggcctcgt gctcgtctgc tcggggcagg agggcgggtt
      241 ttcgatcgag gatacgatct gcgccggcat gctcgtgcac ctgctggcca ccgaacacaa
      301 actgaatctc gcgctgaacg acgccgggtc gctggccctc ctgttgtacc gctcgaacaa
      361 gagcgccctg aagaagacca ttcagcaggg ggagcacggc cgccgactga ccgaactggg
      421 gttcggggcg gacgtggaga cggcggccgc ggtcgacacc atgccagtgc tcccggtcat
      481 ggtcgacggc cgcctggtgg cggacgtgga tccgcccgcg gctccgccgg ccgagccggc
      541 gtgagttcat cgccaaccct tcccgagagt ataagaaagc atgagacacc tgatcattct
      601 cgcggccctg gtcagcctgg aggcaggcgc cccggcggcc gccgcggatg ccgtgcggtt
      661 cgccctcgac gttcaccagc aggactcggc ggccggagcc gtcctgctgt actccgacac
      721 ggcgaccgtg gtgcgcgggg tcgcggcggc cgggtttgcg ctggcgtttt cggcagaagt
      781 ggaagtgacg gcggcggaca ccgcccacgc ccagtttctc gtgcacgcgg tgacgctcgg
      841 accatcggcc agaacctacg cccgcagtta ttcggccgag tacggtctcc ccgcccgcat
      901 cgaggggatc gaggggaagg gaggcgggcg gctgatgctc acggtgacac cgctggcgtt
      961 cacggatttc gacaccgccc agtgcctctt cgaccaccgg gtcaccgggg cctacaagat
     1021 tcagccgagc gccagcctcg acgtgcacta cgtcccgaac tcgttcggcg attactatat
     1081 cgaggctttc aagggcgtcc tcgaacacca ctaccggcag tttcaggaag tgttccagtt
     1141 ctcgctgccg ggcaagttca gcatcttcct tctcccctgc gcctcgccgg cggtgctgtg
     1201 ggacacgcgg tacgggcagg tgaccgatcc gacgcggagc acggcgttcg cgatcgcggc
     1261 caacggtcag aacacggtcg atccgttcat cgccacgcac acggccctcc tgcggagctg
     1321 gggatacgcc ccgccgttct tgtcggaggg ggcggcgaac tacacctcgt tcgccctgtt
     1381 cgacatgaag aagcttgcgg ccgagggccg ggtgccgccg ctggacagcc tgctggacac
     1441 ttacgcctac ctgaccgcgg cgccgctggt agcggaccgc gcctcggcga ccttcgccca
     1501 gtaccttatc gacagctacg ggctggaggc gtttcgcgcg ttgtatgtgt cggcggacga
     1561 tctcaacctc cgccgccgcc tggcgctggc atacggcaag gggatcgggg cgctcgaggc
     1621 cgagtggcgc gcttatgtcg acacgtcgac aattcgcttt cccgcactcg cccaggaggc
     1681 ggacaaggcg gaggccctgt tccagtacgg gcggatgcgg gactacgcgc gggcgatgct
     1741 ggaattggcg ccggggcgga tcgattcggc ctttgcctgg tcgcggctga aacaggccga
     1801 gttcatgctc ggcaactact acggggcggc cgatgcggcc gaagcgcagg tgcagcttga
     1861 cggcgagcgg gcggtcaact ggatgacgct cgggtcgttc caaatgatga acgggatgta
     1921 cgccgaggcc cgcgcgagtc ttcagaacgg ccagtcgaag gacccctcgg atcaaaccat
     1981 cgcgttcaac cgggcgctcg cggcgctcta cacgtccgac accgcgaccg cggaatcgct
     2041 cctggtcgag atcatcaccc gcccggaaag ccgcgcgccg cagggggagg cccgggtggt
     2101 gctgggcgag attctccttt cgcggggcga caagcgcggc cgggaccgcg ccgccaccta
     2161 tttcgtcgag gccatgcaga cgttccagca gcagctctcg gtcaaccgga cgatgccgac
     2221 ggcctacctc tggctggggg cggcggcgct cggcatgggg gacaccgagc tggcgcggga
     2281 cgcgctggag accgggttgt tcctggaatc gcggccgttc tacctggggc agatgaacct
     2341 gtggctgggc aagacggccg acgtcctggg ggaccacggc cttgcgcagg agtactacgg
     2401 gcaggtgctc gccggctcgt cggccgcgta cgcgcagcag g
//
LOCUS       DAOOKS010000274         2440 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_53442_length_2440_cov_3.171908, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000274 DAOOKS010000000
VERSION     DAOOKS010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53442_length_2440_cov_3.171908"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="PK112_05225"
     CDS             complement(<1..270)
                     /locus_tag="PK112_05225"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HPC11484.1"
                     /translation="MAKSVKRIGVLTGGGDCPGLNAVIRAIVKTADNEYDWNVIGFLD
                     GYEGLIDNRYRELAGSDVSGILILGGTILGTSNQADPFHYPVLQDE"
     gene            534..>2440
                     /gene="glgP"
                     /locus_tag="PK112_05230"
     CDS             534..>2440
                     /gene="glgP"
                     /locus_tag="PK112_05230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HPC11485.1"
                     /translation="MRVRQFFVLPKLPERLLPLQELAQNLWYAWSWDLVKLFIRLDAE
                     MWEQCYQNPVEMLSRLPQDALQRAADDDAFVAALHRVYDKYQDYLKAKKWFHYKYGQY
                     EGERIAYFSLEYGLDTSLPVYSGGLGVLSGDTLKSASDIGLPMTAVALLYRYGYFRQF
                     LSSDGWQQERYEENDWYHMPVELVKDANGQPLRITVQMAGAPVHVQVWKTTVGLIPMY
                     ALDTNIPENSTKNREITSVLYGGDKDMRIRQEILLGIGGVRALRALGIEPAIFHMNEG
                     HSWFLALERLRILMQEQGLSYNEALQYVWATTVFTTHTPVPAGNEQFDPVLVHRYLGD
                     FLASLGVPWKDFLTLGRVMPEDESEPFGMTVAALKTSAFANGVSKLHGRVSREMWHNI
                     WPGLPIEEVPIKAITNGVHTQSWISHDMDELYESYLGPRYRERPGAPEVWKRVQKIPD
                     MELWRTHNRRRERLIFFARKRLKQQLQRRGASQLYIQRAEQLLDPQALTICFARRFST
                     YKRGSLIFADLARLERLVNDEKRPVQLIFSGKAHPLDNPGKEIIKYIVGVISEERFRN
                     RIIFLEDYDINVARYMVQGADVWLNNPRRPQEASGTSGMKAALNGALHVSVLDGWWAE
                     GYGDERGFKIG"
BASE COUNT          455 a          792 c          780 g          413 t
ORIGIN      
        1 ctcgtcctgg aggaccgggt agtggaaggg gtcggcctgg ttggaggttc cgaggatggt
       61 gccgccgagg attaggatcc cggagacgtc gctgccggcc agctcccggt accggttgtc
      121 gatgagtccc tcgtagccgt cgaggaaccc gatcacgttc cagtcatact cgttatcggc
      181 ggtcttgacg atcgcccgaa tgaccgcgtt gaggccgggg cagtcgcccc cgccggttag
      241 cacaccgatt cgtttcaccg attttgccat gctgtaaaca aggccgggag ggggttaaaa
      301 acaacctaaa tatttgtcgg cccggccggc cgagagcttg agggaccggc cggggattgt
      361 cgcggcgggg agtgaaccaa accgcccgac gagccgatca catgaggagg accgacaacc
      421 cggccagagg accccgcgcg gtccgggagc cgggaagttt atggttgcat tcgccgctgc
      481 cgaacaatta tctagccccg ggaccgtccg acccgcggaa gcgtaggaga gcgatgcgag
      541 tacgtcaatt ctttgtcctc ccgaagttgc cggagcgcct cctgcccctg caggaattgg
      601 cgcagaatct gtggtacgcc tggagctggg acctggtgaa gctgttcatc cggctggacg
      661 ccgagatgtg ggagcagtgc taccagaacc cggtggagat gctgtcgcgt ctgccccagg
      721 acgccctcca gcgcgccgcc gatgacgacg ctttcgtcgc cgccctccac cgcgtgtacg
      781 acaagtacca ggactacctg aaggccaaaa agtggttcca ctacaagtac ggccagtacg
      841 agggggagcg catcgcctac ttctccctcg agtacggcct ggatacgagt ctgcccgtgt
      901 actccggcgg cctcggggtg ctctcggggg acacgctgaa atcggcctcc gacatcggcc
      961 tccccatgac cgcggtggcg ctcctctacc gctacggcta cttccgccag ttcctgtcgt
     1021 ccgacggctg gcagcaggag cgctacgagg agaacgactg gtaccacatg ccggtggagc
     1081 tggtcaagga cgccaacggc cagccgctcc ggatcaccgt gcagatggcc ggcgccccgg
     1141 tgcacgtcca ggtctggaag acaaccgtcg gcctcatccc gatgtacgcg ctcgacacca
     1201 atatcccgga gaattcgacc aagaaccggg agatcacatc ggtcctctac gggggcgaca
     1261 aggacatgcg catccgccag gaaatccttc tgggaatcgg cggggtccgg gccctgcggg
     1321 cgctggggat cgagcccgcc atcttccaca tgaacgaggg gcactcctgg tttctcgccc
     1381 tcgaacggct gcggattctc atgcaggagc aggggctaag ctacaacgag gcgctccagt
     1441 atgtgtgggc gacgacggtg ttcacgaccc ataccccggt gccggccggc aacgagcagt
     1501 tcgaccccgt cctcgtgcac cgctacctcg gcgacttcct ggcttcgctc ggcgtgccgt
     1561 ggaaggattt tctcaccctc ggccgggtga tgccggagga tgaatcggaa ccgttcggca
     1621 tgacggtggc cgcgctcaag acgtccgcct tcgccaacgg cgtgtcgaaa ctgcacggcc
     1681 gggtgtcgcg cgagatgtgg cacaacattt ggcccggcct gccgattgag gaagttccga
     1741 tcaaggcgat caccaacggc gtgcacacgc agtcgtggat ttcccacgac atggacgagc
     1801 tctacgaatc gtacctcggc ccccgctacc gcgagcggcc gggggcgccg gaggtgtgga
     1861 agcgggtgca gaagattccc gacatggagc tctggcgcac ccacaaccgg cggcgcgagc
     1921 gactcatctt tttcgcccgc aagcgcctca aacagcagct gcagcgccgg ggcgcctcgc
     1981 agctgtatat ccagcgcgcc gagcagttgc tcgatcccca ggccctcacc atctgctttg
     2041 cccggcggtt ttcgacctac aagcgcggca gcctcatctt cgccgacctc gcccgcctgg
     2101 aacgcctggt caacgatgaa aagcgcccgg tgcagctgat cttttccggg aaggcgcacc
     2161 cgctggacaa ccccggcaag gaaatcatca agtacatcgt cggcgtcatc agcgaggagc
     2221 gcttccgcaa ccgcatcatt ttcctcgagg actacgacat caacgtcgcc cgctacatgg
     2281 tgcagggagc ggacgtctgg ctgaacaacc cgcgccggcc gcaggaagcc tcgggcactt
     2341 cgggcatgaa ggccgccctc aacggggcgc tccacgtgtc ggtgctcgac ggctggtggg
     2401 ccgaggggta cggcgacgag cgcggcttca aaatcggcaa
//
LOCUS       DAOOKS010000275         2436 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_53559_length_2436_cov_2.945821, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000275 DAOOKS010000000
VERSION     DAOOKS010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53559_length_2436_cov_2.945821"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="PK112_05235"
     CDS             complement(<1..322)
                     /locus_tag="PK112_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11486.1"
                     /translation="MPTRLLMLGLILAGVAFFSCSDDDEATGWNDEFSFELTAGTVEG
                     DPVPNLRVSIWSLFDGLPIYRRAAGETMEPQVMTVIGFDLPQACSVSLAVYDLAGGLE
                     TTLVA"
     gene            complement(1010..1834)
                     /locus_tag="PK112_05240"
     CDS             complement(1010..1834)
                     /locus_tag="PK112_05240"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPC11487.1"
                     /translation="MSKRVNMLMVAAAVLVMPALVLAGSAHQFAVGKSVASQTEANVV
                     TVPLVVTNEANLAAMDIPLKFSEGVTLQAVEFDGTRTEYFDLKIANINNENRTVIIGL
                     LPQITPDFKPDLEAGTGVVANLVFRIDDPTAQNVSLETFTTENPGHELTFVYHTDDAG
                     QLGQYVVNPEFSTVSFSLTEAAGANLPKQFALNQNYPNPFNPSTTISFALPKPSHVEL
                     SIFNVLGQQVVKLADGQFDAGEHAVTFDASRMASGVYFYRIQTDENTETRKMMLLK"
BASE COUNT          471 a          775 c          668 g          522 t
ORIGIN      
        1 gcgccacgag agtggtctcc agtccgcccg caaggtcgta cactgcaaga ctcaccgagc
       61 aggcctgcgg cagatcgaac ccgattacgg tcataacctg cggctccatc gtttccccgg
      121 cggcgcgccg ataaatcggc agaccgtcaa aaagcgacca tatgctcact ctcagattcg
      181 gcaccgggtc cccctcgacc gtcccggcag tcagctcaaa cgagaactca tcgttccatc
      241 cggtggcctc gtcatcgtcc gagcaggaga agaaggcaac tcccgcaagt atgaggccaa
      301 gcatcagcag acgtgtcggc ataccgacct cctcggtggg cgaatcctgt cagtccctgc
      361 tcaattagta atggaaggat aacgggcggt cgggagaggt gtcaaggggt gatccgtgcg
      421 ccctcggatt tgacgggacc gtcgccggga cgagccgtgg gctgcccaaa aaagaaaggg
      481 cctcgattac tcgaggcctc tctctcctca ccaatcggga aagctcacaa gcagtctaac
      541 cgccaagaaa tactgctcat acttccatgt agtgcgtcag gttgcgggtc tgttcgcact
      601 acgctacccg atgtctcaga tatatacaaa ctccgtgcca atcatcgcct gaaacggcta
      661 tctctttgtc ggtacagatc ttacgtcaaa gcacgattgg tccgatcgcc cgcagaactg
      721 cctttttcgc aaattctgtc ccggcggtgt cgcagaaact gctgtcagtc tcaacttgcg
      781 tctttttcct ggcgaccaga ttggcaccgc catcagccgc cctggagcag ccgtgccccg
      841 catcggttga ctgccggagg ctcgagccgt cgtcacggcc gttgaggcgt cgtttccggc
      901 ggtcgcagcc acaaaaaaga actctccccc gccgtcgcga gggagagttt ccgcagaaaa
      961 ctttcagttc agaatcgcct gcttaacgcc tgcttcacgt atcggccgct tacttcagca
     1021 gcatcatctt ccgcgtttca gtgttctcgt ccgtctgaat gcggtagaag tacacgccgc
     1081 tggccatgcg ggaggcgtca aacgtcaccg catgctcgcc cgcgtcaaac tggccgtccg
     1141 ccagcttcac cacctgctgc cccagcacgt tgaaaatcga cagttcgacg tgcgacggtt
     1201 tgggcagggc gaagctgatg gttgtggacg ggttgaacgg gttcgggtag ttctggttca
     1261 gcgcgaactg cttcggcaga ttcgcgccgg ccgcctcggt cagcgagaac gaaaccgtcg
     1321 agaattccgg gttcacgaca tactggccca gctggccggc atcgtcggtg tggtacacga
     1381 acgtcagctc gtgccccggg ttctcggtcg tgaacgtctc gagggaaacg ttctgcgccg
     1441 tcgggtcgtc aatccggaac accagattgg ccaccacccc ggtccccgcc tccagatccg
     1501 gcttgaaatc cggggtgatc tgcgggagca gcccgatgat gacggtgcgg ttctcattgt
     1561 tgatgttcgc aatcttcagg tcgaaatact cggtgcgggt cccgtcgaat tcgaccgcct
     1621 ggagcgtcac gccctctgaa aacttcagcg ggatgtccat ggccgccagg ttggcctcgt
     1681 tggttaccac gagcggcacc gtcaccacgt tggcttcggt ctggctggca acgctcttcc
     1741 cgactgcgaa ctgatgggcg gacccggcca ggacgagcgc cggcatcacc agcaccgcgg
     1801 ccgcgaccat cagcatgttt acgcgtttac tcaaggttct acctccttgg agttggttgt
     1861 gtctttctcc gtgaccgagc ttccagattc ctccgttgcg ctccttgcca cgtgtatctt
     1921 ccgtaacacg aaatgccagc aagcaatcgt ccgtcagctt gtcactgatt tgcagtcggc
     1981 ggtgttctcg tgggaatctg ttaaataaat acgaaatgat tcgcgatctg tcaaggcgct
     2041 tttccgattt cgaatcactc gcttcgtccg attccctgca caacgtccca gtgggcctgc
     2101 aaccgtccta cgattagtac aacgcccgca cgggcgtagt tccgcttttt tataatgaat
     2161 tttcaatgaa atttcgggaa atcttttccg gtcgcacggc ggtctcgcat cgcatccggc
     2221 gcacaacctg cgccgtgcgg ctccacgttg accccgtttc gccgccgccc cctccccccg
     2281 gtccctctcc ccgcccgccg cagactctgc gattagggct tgacttcgcc ccgtcggggc
     2341 gcaaatttgg gtcgcctgaa atatcgccga cttcaacctg accggctgaa tggacacaag
     2401 gggtcgtccc gccccagcgg agacggtgac caacaa
//
LOCUS       DAOOKS010000276         2435 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_53582_length_2435_cov_3.982773, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000276 DAOOKS010000000
VERSION     DAOOKS010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53582_length_2435_cov_3.982773"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(137..826)
                     /locus_tag="PK112_05245"
     CDS             complement(137..826)
                     /locus_tag="PK112_05245"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HPC11488.1"
                     /translation="MRALLKMSQLFAGLDDASLAEVETATSLRRAVKGEILFSEGDPA
                     ASFFVVGEGKVKVFKLSPDGKEQILMIAKPGDTFAEAAIFNDGRYPADAEAIEDTELL
                     VVNRDRFIALLGRNPNLAFSLIARLCALLRKMATLVEGLALTDVTTRIAHYLARQADR
                     VLKKQPDRAGANAVTVTLPEKKAVLAAQLGTIPETLSRSLAKLTREGIIEVDGPRIHI
                     KDLSRLRGTFE"
     gene            complement(1001..1354)
                     /locus_tag="PK112_05250"
     CDS             complement(1001..1354)
                     /locus_tag="PK112_05250"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPC11489.1"
                     /translation="MAKPILIVDDNPNMSSLLSDMLEVFDYPSERAGDGQEALDKLTA
                     QPFSMVITDMRMPNMTGLELVGRIKQMYPKLPVVLISGYAMSELDHLDGPRPDGFLAK
                     PFMMSDVERLINSLL"
     gene            complement(1407..>2435)
                     /gene="hflX"
                     /locus_tag="PK112_05255"
     CDS             complement(1407..>2435)
                     /gene="hflX"
                     /locus_tag="PK112_05255"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HPC11490.1"
                     /translation="EESLDELAALTVSAGARVVGRRIQVRPAPDPATYIGKGLVEELK
                     NLLTDLGGNCLIFDEPLSPAQQRNLEERLEAKVIDRPILILDLFATRARTAAARLQVE
                     LAQLEYTLPRLTGAWVHFSRQYGGIGAKGPGETQLEIDRRQIRTKIAHLKQRLEKLGT
                     QRAEQRKGRRELFKVALVGYTNAGKSTLFNRLTKADVRTADMLFTTLDSTTRVMSAGY
                     PCRIVFSDTVGFIKKLPHQLVASFKSTLEEVATADLLLHVVDVADPHVDEHIEHTRRV
                     LAEIGAEKIPYLMVYNKIDAKPDSCAANGNHDRTFFISALEGRGLAALQAELIARSSG
                     RPRESSTD"
BASE COUNT          433 a          670 c          880 g          452 t
ORIGIN      
        1 gttcatcatc gtcgccgcct tctggattta ctactcctca attgtgttca tcctgggagc
       61 ggaaatcggg cagctctacc gggagcggcg ggaggcgaag acggggaccg ggtgacgggg
      121 cgggcggggg cgcagcctac tcgaacgtcc cgcggaggcg cgagaggtcc ttgatgtgga
      181 tgcgcggccc gtcgacctcg attatgccct cgcgcgtgag cttggccagc gatcgggaga
      241 gcgtctcggg gatggtcccg agctgggcgg cgaggacggc cttcttttcg gggagggtga
      301 cggtgacggc gttggcgccc gcgcggtcgg gctgtttttt caggacgcgg tcggcctgac
      361 gggcgaggta gtgggcgatc cgggtggtga cgtcggtcag ggcaaggccc tcaaccaggg
      421 tggccatttt ccggagcagg gcgcacaggc gggcgatgag actgaacgcg aggttcgggt
      481 tgcggccgag cagcgcgatg aagcggtcgc ggttgaccac cagcagctcg gtgtcctcga
      541 tcgcctcggc gtcggccggg tagcggccgt cgttgaagat ggccgcctcg gcaaaggtat
      601 ccccgggctt ggcgatcatg aggatctgct ccttgccgtc gggcgagagc ttgaacacct
      661 tgaccttgcc ttcccccacc acgaaaaacg aggcggccgg atccccctcg ctgaagagta
      721 tttccccctt gacggcgcgg cggagggagg tggcggtctc gacctcggcc agcgaggcgt
      781 cgtcgagacc ggcaaagagc tgggacattt tcagtaacgc acgcacgggc ggctccaatt
      841 ctccacatga aggggcgcaa gcaaggtagc tagtgcaggg gtgtctgtaa accgaaaagg
      901 gcggggccgg gcggaccccg tgcggccaat aaaaacccca caccgagtgg tgcggggccc
      961 gggattacta gtggcggatg gtatccagaa tcgcgtggat ctaaagcagg gaattgatga
     1021 gccgctcgac atccgacatc atgaacggtt tggccagaaa cccgtccggc ctgggaccgt
     1081 ccaaatggtc gagctcgctc atcgcatagc ccgaaatcag cacaaccgga agtttcggat
     1141 acatctgttt gatccgcccg acaagttcga gtccggtcat gttcggcatg cgcatgtcgg
     1201 tgatgaccat ggaaaacggc tgggcggtga gcttgtccag cgcctcttgc ccatcgccgg
     1261 cgcgctccga gggatagtcg aagacttcga gcatgtccga caggagcgat gacatattcg
     1321 ggttgtcatc gacaatgaga attggttttg ccatacggtg cctactcctt aggcacagta
     1381 tcggcaggag ttatgtaagc cttgacctaa tctgtcgaag attcgcgggg gcggccggag
     1441 gagcgggcga tgagttcggc ctgcagggcg gccagcccgc ggccttccag agcggagata
     1501 aaaaaggtcc gatcatggtt gccgttggcg gcacagctgt cgggctttgc gtctatcttg
     1561 ttgtatacca taaggtaagg aatcttttcg gcgccgatct cggccagcac ccgccgggtg
     1621 tgctcaatgt gctcgtccac gtgggggtcg gccacgtcga ccacgtgcag caggaggtcg
     1681 gcggtggcca cctcctctag cgtcgacttg aacgaggcca cgagctgatg cggaagtttc
     1741 ttgatgaagc cgacggtgtc ggagaagacg atgcggcagg gataaccggc ggacatgacg
     1801 cgggtggtgg agtcgagagt ggtgaagagc atgtcggcgg tgcggacatc ggccttggtc
     1861 agccggttga agagagtcga tttgccggcg ttggtatagc cgaccagagc cactttgaag
     1921 agctcgcggc ggcccttgcg ctgttcggcg cgctgggtgc cgagtttctc gaggcgctgt
     1981 ttgaggtggg cgatcttggt ccggatctgg cggcggtcga tctcgagctg ggtctcgccg
     2041 gggcccttgg cgccgatccc gccgtactgg cgcgagaagt gcacccaggc gccggtgaga
     2101 cgcgggaggg tgtattccaa ctgggcgagt tcgacctgga ggcgcgcggc cgccgtgcgg
     2161 gcgcgggtag caaacaggtc gaggatgagg atgggccggt cgatgacttt cgcctccagg
     2221 cgctcctcga ggttgcgctg ctgcgccggc gagagaggtt catcgaaaat caggcaattg
     2281 ccgccgaggt cggtaaggag gttcttgagc tcctcgacaa ggcctttacc gatgtaggtg
     2341 gcgggatcgg gggcgggacg gacctgaatg cgccgtccga caacccgggc gcccgcggag
     2401 accgtcaagg cggcgagctc atcgagcgat tcctc
//
LOCUS       DAOOKS010000277         2428 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_53818_length_2428_cov_2.756848, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000277 DAOOKS010000000
VERSION     DAOOKS010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53818_length_2428_cov_2.756848"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="PK112_05260"
     CDS             complement(<1..462)
                     /locus_tag="PK112_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPC11491.1"
                     /translation="MARIIAIANQKGGVGKTTTAVNLSSCLAAAELKTCLIDMDPQAN
                     STSGFGFDKRTIETSIYDVLLGSATLREVLRETELSYLHLAPSSIALVGAEVELVSVM
                     SRETRLRAALAPVLDDYAYILVDCPPSLGLLTVNALTAAHSVIIPVQCEYYA"
     gene            complement(550..1200)
                     /locus_tag="PK112_05265"
     CDS             complement(550..1200)
                     /locus_tag="PK112_05265"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPC11492.1"
                     /translation="MNGTSEGIRLDVAAFLRERDPNNRLTPYFELLARENSRVNIVSR
                     ETLPPTAPSGRDRFEGLRRLTAESIYPITELHRAQTRSYLDIGSGGGFPAIPIVLTAT
                     ISDTLLVERTRKKAAALRRILMELQLRAEICTESFESLVLKEGSCDLITLRLVRLTDR
                     LAARILPLLAPGGDFVYYALADISADSSRFLTRQLRFTAGGGRDFHTATIVTRKPE"
     gene            complement(1197..>2428)
                     /locus_tag="PK112_05270"
     CDS             complement(1197..>2428)
                     /locus_tag="PK112_05270"
                     /inference="COORDINATES: protein motif:HMM:NF025300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPC11493.1"
                     /translation="QVQPGDEPVPFFSPRSPRRVFQQVCCHLTYTTSATKAIIGENFH
                     LSPMFSGQIKSRGPRYCPSIEDKFFRFADKERHQLFLEPEGNGTNEVYPNGFSTALPE
                     EVQVKAIRTVAGLENVRVTRPGYAVEYDYCPAHQIKPSLETRRINGLFLAGQINGTSG
                     YEEAAGQGIMAGINAVLRIEQEAPFVLDRSEAYIGVMIDDLTTRSTTEPYRMFTSRAE
                     YRLTLREDNARDRLQSYARRFGLIPETVLAGFDDLQRRTDAVVAMIPKIRVRVDELGE
                     AGARFTRVETISLDNLLRQPTLSINEAVAWLRKFPGRFPDDQEALERAAIVVRYSGYV
                     DKQQREIDKFRRMERETIPDDFDFAGVSGLKKEAGEKLARFRPTSLGQAGRIEGITPG
                     DVAVLSVYLQRHKALSA"
BASE COUNT          465 a          773 c          719 g          471 t
ORIGIN      
        1 ggcgtagtac tcgcactgga cggggatgat cacgctgtgc gccgccgtca gcgcattgac
       61 ggtgagcaac ccgagcgacg gcgggcagtc gacgaggatg taggcgtaat catcgagcac
      121 cggggccagc gccgcgcgca ggcgcgtctc ccgcgacatc accgacacca gctcgacctc
      181 cgcccccacc agggcgatcg atgaaggcgc caggtggagg tacgagagct ccgtctcccg
      241 cagcacctcg cgcagcgtcg ccgagccgag caggacgtcg tagattgatg tctcgattgt
      301 tcgtttgtcg aatccgaacc cgctggtgga gtttgcctgc gggtccatgt cgatcaggca
      361 ggttttcaat tcggccgcgg ccaggcagga actgagattc accgccgtgg tcgttttgcc
      421 cacgccgccc ttttgattcg cgatcgcgat aattctggcc aagcaacaat ccttgttgac
      481 gatatccaca cactcgaaaa ccgaatacta tgcgcagaga ccctcagaat caactaaaat
      541 gtgaaagagt cactcaggtt ttctcgtaac tatcgtggcc gtgtggaaat cccgcccccc
      601 gccggcggtg aatcgcagtt gtctggtcag aaatctggag gaatcagccg agatatcggc
      661 cagtgcatag tagacgaaat ctccgcccgg cgccaacagc ggaaggattc gcgcggccag
      721 tcggtcggtg agccgcacca accgaagcgt gatcagatcg catgatcctt ccttaaggac
      781 gagcgactcg aacgattcag tgcaaatctc cgcccgaagc tgcagctcca tcagtatccg
      841 gcgcagcgcg gcggctttct tccgtgtccg ttccaccagg agggtgtcgc tgatcgttgc
      901 ggtgagcaca atggggatcg ccgggaatcc tccaccgctg ccgatgtcga gataactcct
      961 cgtctgcgct cggtggagtt cggtgatcgg gtagatcgat tccgccgtga gccgccgcag
     1021 tccctcgaac cgatcgcgcc ccgacggggc ggtcgggggc aatgtttcac gtgaaacaat
     1081 gttgacccgg ctgttctccc gcgcgaggag ctcgaaatac ggggtgagcc tgttattagg
     1141 atcccgttcg cgcagaaacg ccgccacgtc aagcctgatc ccctcgcttg tcccgttcat
     1201 gccgacagcg ccttgtgcct ctgaaggtag accgaaagca ccgctacatc ccccggagtg
     1261 atcccctcga tccgccccgc ctggcccagc gaggtcggcc gaaaccgcgc cagtttctcc
     1321 ccagcttcct tcttaagccc gctcacgccc gcgaaatcga aatcatcggg aattgtctcc
     1381 cgctccatgc gccggaattt gtcgatttcc cgttgctgct tgtccacgta cccgctgtaa
     1441 cgaaccacga tggctgccct ctcgagcgcc tcctggtcat ccgggaaccg gccggggaac
     1501 ttccgcagcc acgccaccgc ctcattaata gagagcgtcg gctgacggag gaggttgtcg
     1561 aggctaatcg tctccactcg cgtgaaccgg gctcctgctt caccaagttc atccacccgc
     1621 acgcggatct tcgggatcat cgccaccacc gcatcggttc ggcgctgcag atcatcgaaa
     1681 ccggcgagta ccgtttccgg aatgagaccg aaccgccggg cgtaggactg gagccggtcg
     1741 cgggcgttgt cttcgcggag cgtgagccgg tactcagccc gcgaggtgaa catccggtaa
     1801 ggctcggtag tcgaccgcgt ggtcaggtcg tcgatcatca ccccgatgta cgcctcggag
     1861 cggtcaagta caaacggcgc ttcctgttcg atccggagaa cggcgttgat gccggccata
     1921 atcccctgtc ccgcagcttc ttcgtatccc gatgtcccgt tgatttgtcc ggcgaggaac
     1981 agcccgttga tccgcctggt ctccagcgac ggcttgatct ggtgagccgg gcagtagtca
     2041 tactcgaccg cgtaccccgg acgggtcacc cggacattct caagcccggc gaccgtccgg
     2101 atcgccttca cctgcacctc ttcggggagc gcagtggaaa agccgttcgg atatacttca
     2161 ttggtcccgt tcccctccgg ctcgaggaaa agctgatggc gctccttgtc ggcgaagcgg
     2221 aagaatttat cctcaatcga cgggcagtag cgcggtccgc gcgatttgat ctggccggaa
     2281 aacatcggag acaggtggaa gttttcgccg attattgcct tagtcgccga ggtcgtgtac
     2341 gtcaggtggc agcaaacctg ttggaacacc cggcgaggcg agcgggggga gaaaaacggc
     2401 accggctcat cccccggctg aacctggc
//
LOCUS       DAOOKS010000278         2423 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_53978_length_2423_cov_3.548564, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000278 DAOOKS010000000
VERSION     DAOOKS010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53978_length_2423_cov_3.548564"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="PK112_05275"
     CDS             <1..473
                     /locus_tag="PK112_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HPC11494.1"
                     /translation="IHPPQSQRVDYEAELGVIIGKEARRVPADRAADYIFGLTCVNDV
                     TARDLQKTDGQWSRAKGFDTFCPVGPWAVCGLDYSDLLVEGILNGEIMQSGRTSRMIF
                     KIPELISYVSSVMTLLPGDIISTGTPAGIAPMKPGDRIEVRVEGIGSLVNQMAE"
     gene            528..1718
                     /locus_tag="PK112_05280"
     CDS             528..1718
                     /locus_tag="PK112_05280"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HPC11495.1"
                     /translation="MTERLYYTDPDLLRFEARILDAGREGGRYFAVLDRTAFYPTSGG
                     QLFDTGILNSVSIVDVTESSDGTIRHYSPQAIGEVGAVVSGAVDAARRKRHRRQHTAQ
                     HILSHAFLQLHGCETVSVHLGEEYGAIEFDTGALSDDQLLAVEERAEEIIFADLPVDI
                     LFVPPEQIATIPLRRPPKKEGILRVIRIGDIEYSACGGTHCRRTGQVGLVKIIGVDKM
                     RGHALVNFLAGDQARDDYAARLEVTARLTRHFTCNVRDLGDKVLKLEAEKRGLHQEVV
                     RLQRELLPSQADSLAGQATAVGERLVLVAELPHYDPKLALQLAALLAERINGLAVLLV
                     ESRLYIAVGPGSGLHAGNIVKALGAATGLRGGGGPLQAQLGGADPAQLTTYRALAEKL
                     VREA"
     gene            1708..>2423
                     /locus_tag="PK112_05285"
     CDS             1708..>2423
                     /locus_tag="PK112_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11496.1"
                     /translation="MRRNRAIVTAILFLLAAAGAAAQNRQGFVLERSNQLEVVWENER
                     YVTYVDGEVKFRTETGAVYCDSATFVRGEYAKLRGRVLIDDQAYMLRADSVYYDLTRE
                     TALALGSQVQLWSYADSLYAAGVHAFYDRPAQHFEMQERPTVYLGYPDTAAMVEVVAN
                     TVNYFAPARRAEAVGRVVITSQDIAAAAECAVMLMDRNTLDLYDGPTAKRGRSTMTGG
                     FMSIAFGEDQLREIDVIDSAR"
BASE COUNT          393 a          831 c          797 g          402 t
ORIGIN      
        1 tcatccatcc gcctcagtcg cagcgcgtgg actacgaggc cgagctgggg gtcattatcg
       61 gcaaggaggc ccgccgcgtg ccggccgacc gcgccgccga ctacatcttc gggctcacct
      121 gcgtgaacga cgtcaccgcc cgcgacctgc agaagaccga cggccagtgg tcgcgcgcca
      181 aggggttcga caccttctgc ccggtcggtc cctgggccgt ctgcggcctc gattactccg
      241 atctcctggt ggagggaatc ctgaacggcg agatcatgca gtcggggcgg acgagccgga
      301 tgattttcaa aatcccggag ctcatcagct acgtctcctc ggtcatgacg ctgctgcccg
      361 gcgacatcat ttcgaccggc acgcccgccg ggattgctcc tatgaagccg ggtgaccgga
      421 tcgaggtgcg tgtggagggc atcggctcgc tggtcaacca gatggccgag taggcgagga
      481 ggccgcggcc gggtacaaag gatgaggctg ttggaggaac gccacgcatg acggagcgac
      541 tctactatac cgatccggac ctcctgcggt ttgaggcccg cattctcgat gccggccgcg
      601 agggcggccg gtacttcgcc gtcctcgacc gaaccgcgtt ctaccccact tccggcgggc
      661 agctcttcga taccggcatt ctcaacagcg tctccatcgt cgatgtcacc gaatcctccg
      721 acggaacgat tcggcactat tcgccgcagg cgatcggaga ggtcggggcg gtcgtgagcg
      781 gagcggtgga cgccgcccgc cgcaaacgcc accgccggca gcacaccgcg cagcacatcc
      841 tgagccacgc ttttctgcag ctccacggct gcgagacggt ctccgtgcac ctcggcgagg
      901 agtacggcgc cattgagttt gacaccggcg cgctgtccga tgaccagctc ctcgcggtcg
      961 aggaacgcgc cgaggagatt atcttcgccg acctccccgt ggacatcctc tttgtgccgc
     1021 cggagcagat cgcgaccatc ccgctgcgcc ggccgccgaa gaaggagggg attctccgcg
     1081 tgatccgcat cggcgacatc gagtattccg cctgcggcgg gacgcactgt cgccggacgg
     1141 gccaggtcgg cctggtgaag atcatcgggg tggacaaaat gcgcggccac gccctcgtca
     1201 acttcctcgc cggcgaccag gcccgcgacg actacgcggc ccggctggag gtgaccgccc
     1261 gcctgacccg gcactttacc tgcaatgtcc gggatctcgg ggacaaggtg ctgaaactcg
     1321 aggcggagaa gcgcggcctc caccaggagg tggttcgttt gcagagagaa ctgctgccgt
     1381 cgcaggccga cagcctcgcc gggcaggcca cggcggtggg ggagaggctc gtcctggtgg
     1441 ccgaactgcc gcactacgat cccaagctgg cgctgcagct ggcggccctg ctggccgagc
     1501 ggatcaacgg cctggcggtg ctcctggtcg aaagccggct ctacattgcg gtggggccgg
     1561 gcagcggcct gcatgcgggc aatattgtga aagcgctggg cgccgccacc ggtctgcgcg
     1621 gcggcggcgg gccgctccag gcgcagctcg gcggcgccga cccggcccag ctcaccacct
     1681 atcgtgctct tgcggagaag ctcgtgcgtg aggcgtaacc gggccatcgt caccgccatc
     1741 ctcttcctgc tggccgccgc cggcgcggcc gcccagaacc ggcagggctt tgtcctcgaa
     1801 cggagcaacc agctcgaggt ggtctgggaa aatgagcgct atgtcacgta tgtcgacggc
     1861 gaggtcaagt tccgcaccga gaccggcgcc gtctactgcg actccgccac cttcgtccgc
     1921 ggtgaatacg cgaaactccg cggccgcgtg ctcatcgacg accaggcgta tatgctccgc
     1981 gccgactcgg tctactacga tctgacccgg gagacggcgc tcgctctggg ttcgcaggtg
     2041 cagttgtggt cctatgccga ctcgctctac gcggccggcg tccacgcctt ttacgatcgc
     2101 ccggcgcagc atttcgagat gcaggagcgc cccaccgtgt acctcggcta ccctgacacc
     2161 gcggcgatgg tcgaggtggt ggcgaacacg gtcaactact tcgctcccgc gcgccgcgcc
     2221 gaggcggtcg ggcgggtcgt catcacctcg caggatattg cggccgccgc cgagtgcgcc
     2281 gtcatgctta tggatcggaa cactctcgat ctctatgacg ggcctacggc caagcgcggc
     2341 cgctccacca tgaccggcgg cttcatgtcc atcgctttcg gcgaggacca gctccgcgaa
     2401 atcgacgtca tcgactccgc ccg
//
LOCUS       DAOOKS010000279         2413 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_54305_length_2413_cov_3.162002, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000279 DAOOKS010000000
VERSION     DAOOKS010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54305_length_2413_cov_3.162002"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2065
                     /locus_tag="PK112_05290"
     CDS             <1..2065
                     /locus_tag="PK112_05290"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate binding family 9 domain-containing
                     protein"
                     /protein_id="HPC11497.1"
                     /translation="SPGDMTPPAVRTEVLVTYDKANLYVGFICDDDPRALRATMTRRD
                     AYSGDDCAIVLIDTYGNAASAYEFFVNPYGVQKDILWTPTVGEDASFDLVWRSAAQVT
                     PEGYRVEIAIPFSGLRFPQGAEQSWRVDFWRNRPRASSFQYSWAAYNRSEQCWPCQWG
                     TVEGIADVAPGKGVELLATAVANQSGQIVAAEGYPFDNEKVEGEMSLAGKYAVTSGMT
                     VEATWNPDFSQIESDAAQIDINSTIDLLYPERRPFFQEGADIFQTLFNSFSSRTIWDP
                     RYAAKLTGRAGRWRLGFLSARDENSPYTVPLEESGGSLRAGASTVNVLRGSLSFGQDS
                     RGGFILTDRRYDWGGYGTVASLDADIRLSRNYSWVGQVVASYTGEPDRPELFGQPERF
                     EGMTFDNGKHTVLTDGESFSGNALITQFRRQGRHLNFRLNYDQVDPTYRTQTGYDPWN
                     DYRNAFVNAYYLFYGEGRLIERLAPNIYVDRRWNYDGRIKWTHGTAGLEANFRFWQSY
                     ASLTYTAGDENWGGVQFDGLYAVRIGAGGVISNALAFNGDLSFGRDVAVRQSATGNQV
                     TGGIEVDLKPLDRLMIEPSYTFTRSRHVATDELLYEQHITRTRVQFQATRELSLRLVV
                     QYNDGSERWDIDPLLSYQLSPFSVFYVGTTYDYQNLAAGTDEPDQWRMSSRRFFLKLQ
                     YLFQT"
BASE COUNT          421 a          779 c          802 g          411 t
ORIGIN      
        1 ctcgcccggg gacatgacgc cgccggcggt gcgcacggag gtgctcgtca cctacgacaa
       61 ggccaatctc tatgtcgggt tcatctgcga tgatgatccc cgggcgctcc gggccacgat
      121 gacccggcgc gacgcataca gcggcgacga ctgcgcgatc gtgctgatcg acacgtacgg
      181 caacgccgcc tcggcctatg aattcttcgt caatccctat ggtgtgcaga aggacatcct
      241 ctggacgccg acggtcggcg aggacgcgag tttcgacctg gtgtggcggt cggcggcgca
      301 ggtcaccccg gagggttacc gggtggagat cgcgatccca ttttccgggc tgcgctttcc
      361 ccagggggcc gagcagtcgt ggcgggtgga cttctggcgc aaccgcccgc gtgcgtcgtc
      421 cttccagtac tcctgggcgg cctacaaccg gagcgagcag tgctggccct gccagtgggg
      481 gacggttgag gggatcgccg acgtggcgcc cgggaaggga gtcgaattgc tcgccaccgc
      541 ggtggccaac cagtcgggac agatcgtcgc cgccgagggg tacccgttcg acaacgagaa
      601 ggtcgagggg gagatgtcgc tggccgggaa gtacgcggtg acctccggga tgacggtgga
      661 ggcgacctgg aatccggatt tcagtcagat cgagtcggac gccgcccaga tcgacatcaa
      721 ttcgaccatc gacttgctct atcccgagcg gcggccattt ttccaggagg gggcggacat
      781 tttccagacg ctcttcaact cgttttcctc gcgcacgatc tgggatcccc ggtacgcggc
      841 gaaactcacc ggccgggccg ggcggtggcg gctcggattt ctcagcgccc gggatgagaa
      901 ctcgccctac accgtgccgc tggaagagag cggggggagc ctccgcgccg gcgccagcac
      961 cgtcaacgtc ctgcgcggct cgctctcgtt cgggcaggac tcgcgcggcg gtttcatcct
     1021 caccgaccgc cgctacgact ggggggggta cgggacggtg gcgagcctcg atgcggacat
     1081 ccggctctcc cgcaactaca gctgggtcgg gcaggtggtc gccagctaca ccggcgaacc
     1141 cgaccggccc gaattgttcg ggcagcccga gcgcttcgag ggcatgactt tcgacaacgg
     1201 caaacacacg gtgctcaccg acggcgagtc gttctccggc aacgccctca tcacccagtt
     1261 ccgccgccag gggcggcacc tcaatttccg tctcaactac gaccaggtgg atccgaccta
     1321 tcggacccag accggctacg atccctggaa cgactaccgg aacgcgttcg tcaacgcgta
     1381 ctaccttttc tacggcgagg gccgtctgat tgagcgcctg gcgccgaata tctatgtcga
     1441 ccggcgctgg aactacgacg gcaggatcaa atggacgcac ggcacggccg ggctggaggc
     1501 gaatttccgc ttctggcaga gttatgcctc gctcacttac acggccggtg acgagaattg
     1561 ggggggcgtg cagttcgacg gtctctacgc cgtgcggatc ggcgccggcg gcgtgatcag
     1621 caatgcgctc gcgttcaacg gcgacctgtc gttcggccgc gacgtggccg tacggcagtc
     1681 ggccacagga aatcaagtga ccggggggat cgaggtcgac ctcaagccgc tcgaccggct
     1741 gatgatcgaa ccgagctaca ccttcacccg cagccgccac gtcgccaccg acgaactcct
     1801 gtacgagcag cacatcaccc ggacccgcgt ccagttccag gccacccggg agctgtcgct
     1861 gcggctggtg gtacagtaca acgacgggtc ggagcggtgg gatatcgacc cgcttctctc
     1921 ctaccagctc agcccgtttt ccgtcttcta tgtcgggacg acctacgact accagaatct
     1981 cgcggccggg actgatgaac cggaccagtg gcggatgtcg tcgcggcggt ttttcctgaa
     2041 actgcaatac ctcttccaaa catgacccct tcttcgaggg aggctgcagc cggcggcgga
     2101 accccggggt ttcgccgcct ttctatgccc ggcgggagcc gccggaccgc ccagaaaatc
     2161 tcttgacagc ctggccccgc ggcgtgtaga attttgccgg ctcaaccggg aggtccggag
     2221 acgcagggcg ggggcgggga cacggttatt actcgaatag cgtgatgatg actcggaggc
     2281 gcgccgcgcg gggctcgccg cgcgccgggc aatcggggtg ccgggcatca tccggtgcgg
     2341 cgaagggagg tgagaccggg cacaggaaat ctgttcgtcc acgactacgc gcacacgttt
     2401 caaaacttcc agg
//
LOCUS       DAOOKS010000280         2410 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_54405_length_2410_cov_3.365180, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000280 DAOOKS010000000
VERSION     DAOOKS010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54405_length_2410_cov_3.365180"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="PK112_05295"
     CDS             complement(<1..684)
                     /locus_tag="PK112_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007465476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11498.1"
                     /translation="MLVYAVVTAYRTWLVALLLAAVIVAAVKPAVEVGVRQVLPPVRQ
                     SKMLGLVKREKPRPAALAVTRLLMLAAWAGVGGVVFLRWRRELPDRLAAAEACARRWE
                     HEAERLAETAPDRSRRLYRAALVLTLDREARRRVQARLCAPGGAADAAATCAATPADT
                     LAPPTRAGAEGPAPPGLDRRYRLEEKLGQGAMGIVYRGHDQVLDRPVALKRLAARLVG
                     ESVYSERYRQ"
     gene            1036..>2410
                     /locus_tag="PK112_05300"
     CDS             1036..>2410
                     /locus_tag="PK112_05300"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014558880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 4 subunit A"
                     /protein_id="HPC11499.1"
                     /translation="MTMERQKIETIFLEEEMKHSYLDYSMSVITNRALPDIRDGLKPS
                     NRRILVAMNDLNLAPGRPHRKCAKICGDTSGNYHPHGEQVVYPTLVRMAQDFNMRYPL
                     VDGQGNFGSIDGDGAAAMRYTEARLTPMAMEMLDDLKKETVDFMPNYDSTREEPVVLP
                     AKFPNLLCNGTSGIAVGMATNIPPHNLNEIAEAIIRVIDDPECTNDDLMEIVPGPDFP
                     TGGIINGRDGIREAYRTGKGHVYVRARAAIERMKNGKDAIIVSEIPFQVNKTNLLEKI
                     AELVRDKKIDGIADLRDESDRDGMRIFIELKRDIPPDVVLNQLFTHTTMQTTFSVNML
                     GLDHGVPKQMSLKQLIRAFVDHRHEVVVRRTQYDLRKAEERAHILEGYRIALDHIDEV
                     IALIRKSKDTPTAHAGLMKKFGLSDVQATAILEMRLQRLTGLERQKIEDEYRELIQKI
                     SEYRAILE"
BASE COUNT          467 a          844 c          740 g          359 t
ORIGIN      
        1 ctggcggtag cgctcgctgt agaccgactc gccgacgagc cgcgcggcga gccgcttgag
       61 cgccaccggg cggtcgagca cctggtcatg gccgcgatag actatgccca tcgccccttg
      121 gccgagtttc tcctccaacc ggtagcggcg gtcgagaccc ggcggcgccg gcccttccgc
      181 gcccgcccgg gtgggcgggg ccaaggtgtc cgccggggtc gcggcgcatg tcgcggcggc
      241 atcggccgct ccgcccggag cgcacagccg ggcctgcacc cgccgccggg cctcccgatc
      301 gagcgtcagc accagcgcgg cccgatacag ccgccggctg cgatcgggcg ccgtttcggc
      361 cagacgctcg gcctcgtgct cccaccggcg ggcacatgcc tccgccgcgg cgagccggtc
      421 cggcaattcg cggcgccacc ggagaaacac gaccccgccg acgcccgccc aggccgccag
      481 catgagcagg cgcgtgaccg cgagagccgc cggacgaggc ttctcccgct tcaccagccc
      541 gagcatcttg ctctgtctga ccggggggag aacctgccgg acgccgactt ccaccgccgg
      601 cttcaccgcc gccactatca cggccgccag cagcagcgcg accagccagg ttcgatatgc
      661 ggtgaccaca gcataaacga gcaccggcgg tctccttagc agcggcccca gcggcgcgcg
      721 ggacacggga gcggtgattc ttatggacgc ttggtcaaac tgcggacgtg tcgccatagt
      781 ccattgatcg acattcccag gcggcgtcat aagcgatcgg cctcggctag ccgcacgtcg
      841 ggcagcgcgg ccggcggaag cccgatgccg ccacccgtcc gccgttcggg aagcggccgc
      901 taatacattg tcgcacaatc ggataatgcc aggctttcgg cggtttttta ttgctattcg
      961 cgcatataat ggttagctta tcgtcagcgt atcgtggact ttacgactgc aagatagaag
     1021 ggaccttcgg cggccatgac aatggagcgg caaaagatag agacgatctt cctcgaagag
     1081 gagatgaagc actcctacct ggattactcg atgtcggtga tcaccaatcg ggcgctgccc
     1141 gacatccgcg acgggcttaa gccgtcgaac cgacgcatcc tggtggcgat gaacgatctg
     1201 aatttggcgc ccggacggcc gcaccgtaag tgcgccaaga tctgcggcga cacctcggga
     1261 aattaccatc cccacggcga gcaggtggtg tacccgacgc tcgtgcgcat ggcgcaggac
     1321 ttcaacatgc gctatccgct ggtggacggc cagggaaact ttggctcgat cgacggcgac
     1381 ggcgcggcgg ccatgcggta cacggaagcc cgcctgacac cgatggcgat ggagatgctc
     1441 gacgatctga aaaaagagac cgtcgacttc atgcccaact acgacagcac gcgggaggag
     1501 ccggtggtcc tgccggccaa gttccccaat ctcctgtgca acggcacctc cggcatcgcc
     1561 gtaggcatgg cgaccaacat tccgccgcac aacctcaacg agatcgccga ggccatcatc
     1621 cgcgtgatcg acgatcccga gtgtacgaac gacgacctca tggagattgt ccccggcccc
     1681 gatttcccca ccggcgggat catcaacggc cgcgacggca tccgcgaggc ctaccgcacc
     1741 ggcaaggggc acgtgtacgt gcgcgcccgc gccgccatcg agcgcatgaa gaacggcaag
     1801 gacgccatca tcgtctccga aatccccttc caggtcaaca agaccaacct gctggagaag
     1861 atcgccgagc ttgtgcgcga caagaagatc gacggcatcg ccgacctccg cgacgagtcc
     1921 gaccgcgacg gcatgcgcat tttcatcgag ctcaagcgcg acattccgcc cgacgtcgtc
     1981 ctcaaccagc tcttcaccca cacgaccatg cagaccacct tctcggtcaa catgctcggg
     2041 ctcgaccacg gcgtgccgaa acagatgagc ctcaaacagc tcatccgggc gtttgtcgac
     2101 caccgccacg aagtggtcgt gcggcgcacc cagtacgacc tgcgcaaggc cgaggagcgg
     2161 gcccacatcc tcgagggcta ccgcatcgcg ctggaccaca tcgacgaagt catcgccctc
     2221 atccggaagt cgaaagacac gccgaccgcg cacgccgggc tgatgaagaa gttcggcctg
     2281 tcggacgtcc aggcgaccgc gatcctcgag atgcgcctcc agcgcctcac cggcctcgag
     2341 cggcagaaga tcgaggacga gtaccgcgag ctgatccaga agatctccga gtaccgcgcc
     2401 attctggagt
//
LOCUS       DAOOKS010000281         2410 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_54409_length_2410_cov_2.605520, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000281 DAOOKS010000000
VERSION     DAOOKS010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54409_length_2410_cov_2.605520"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1338
                     /locus_tag="PK112_05305"
     CDS             <1..1338
                     /locus_tag="PK112_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11500.1"
                     /translation="YDAWIETETLPNGHLLVRVVTIDEWTLAFGPSVRTEGGDHEYEF
                     LLTERNVLGRNQEVRARYVFGNSEKDFVDLAYTNQRMFGRPVRAAGGYRTDPVNGVKW
                     VELSHPYYNLTQSFTYRAEVGASEVRRDVYSDDLLIGQSFREGDWCELGIGSRTGTYR
                     RKLELSCRYYYRFDRTVGTALIDTDPDLMEQALRSLPDDSAYHLVEVGTAFSNLDFTT
                     LTRINGFSYTEDFELGYGLGISYGRAFLPDFDRHAYDRLSASVSHGRRFGSNLWYGSW
                     RYTVWLRGERDLRRLSDVYSRFYRRWFEFLTLAMGFSYRSDWRADGTEGLVMGGETGL
                     RGYDQYFRTGDRMAVLNLEARFFPDVRIHSAVFSPVLFTDFAQRWKRGEAFGTGDVYG
                     AIGAGLRIALEHSARARLVRVDASYSEVNSWRFSISAEQYFIAPRSGFFLTSP"
     gene            1583..>2410
                     /locus_tag="PK112_05310"
     CDS             1583..>2410
                     /locus_tag="PK112_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11501.1"
                     /translation="MRKRTFLWFVILGLGLAAGSTASAVSDDPLALIAQDYRAGAITL
                     DQKVLLQIQAITSPAALPDRYVSAGLAASGGALTRCATPVILEIRREWELLQPETKAV
                     FSAAFQRAVTEAEYLSPSGWFKLHYDTTGADAVPPEDADMTGVPDLVEKCAAYLDSSR
                     TRHLDYGYRMPPPDSGLGGDDLFDVYFEETGAYGYAMPEGPGPEPWNDVYSYLVLNND
                     FLGFAPNYDPEGDQLGAAKATCAHEFHHCVQFAYDSNEDSWFMEMDATHIEDIVFDAT
                     "
BASE COUNT          408 a          750 c          806 g          446 t
ORIGIN      
        1 tacgacgcct ggatcgagac ggaaaccctg cccaacggac acctgctcgt gcgggtggtg
       61 acgatcgacg aatggacgtt ggcgttcggg ccgagcgtgc gcaccgaggg cggggatcat
      121 gaatacgagt ttctgctgac cgagcgcaac gtgctcgggc gcaaccagga ggtcagggcg
      181 cgctacgtct tcggcaacag cgagaaagac ttcgtcgacc tcgcctacac gaaccagcgc
      241 atgttcggcc gcccggtccg ggcggccggc gggtaccgca ctgacccggt gaacggcgtg
      301 aaatgggtcg agctgagcca cccctactac aacctcaccc agagcttcac ctaccgggcc
      361 gaggtgggcg cgtcggaagt ccgccgggac gtatacagcg acgacctcct catcgggcag
      421 tcgtttcggg agggggactg gtgcgaactg ggcatcggat cccggaccgg cacctaccgg
      481 cgcaagctgg agctttcctg ccgctactac taccggtttg accggacggt ggggaccgcg
      541 ctgatcgaca ccgatcccga tctgatggag caggcgctgc gatcgctgcc ggacgactcc
      601 gcctaccacc tcgtcgaggt ggggaccgcc ttctccaacc ttgacttcac gacgctgacg
      661 cggattaacg ggttctcgta taccgaggat tttgaactgg gttatggcct gggcatctcc
      721 tacggccgcg ccttcctgcc cgattttgac cggcatgcct acgaccggct gtcggcgagc
      781 gtttcccatg gccgccggtt tgggtccaat ctgtggtacg gttcctggcg gtacacggtg
      841 tggctcaggg gggaacgcga tctccggcgg ctgagcgacg tctacagccg cttctatcgc
      901 cggtggttcg agttcctcac gctggccatg gggttcagct accggtccga ttggcgggcc
      961 gacggcaccg agggtctggt catgggaggg gagaccgggc tgcgcggtta tgaccagtac
     1021 ttccgcaccg gcgaccggat ggcggttttg aacctggagg cgcgcttttt ccccgacgtg
     1081 cggatacact cggccgtgtt ctcaccggta ctgttcacgg actttgcgca gcggtggaag
     1141 cggggggagg cgttcgggac gggggacgtc tacggggcaa tcggggccgg cctgcggatt
     1201 gcgttggagc actcggcccg ggcccggctg gtgcgggtgg atgcctccta ttcggaggtg
     1261 aattcctggc ggttttcgat ctcggccgag cagtatttca tcgccccccg ctccggattc
     1321 ttcttgacaa gcccctgaaa tacaataaat tatggtggac cgtggaaatc taccccaccg
     1381 gcgcgccgcg ctcccgtttg cccgcgagct cgtggcggga acggcgcagg atcggccgct
     1441 gttggtagaa tacttcgtgg tccgcccaag gtctgcggaa gcaagaaaaa gccctgttac
     1501 gcacgttgca ctggatatgt cggcggtcgg accgatactc tgatggtaag gaagcgcccc
     1561 gggcgctccg ggtggagaat tgatgcgcaa gcgcacgttc ctctggttcg tcatccttgg
     1621 cctcgggctc gccgccgggt cgacggcgag cgctgtttcc gatgatcctc tcgcgctcat
     1681 cgcgcaggac taccgggccg gcgcaatcac gctcgaccag aaagtgctgc tgcagatcca
     1741 ggccatcacg agccctgcgg ctctgcccga ccgttacgtt tccgccgggc tggccgcgag
     1801 cggcggcgcg ctcacccggt gcgcgacccc ggtaattctg gagatccggc gcgagtggga
     1861 attgctgcag ccggagacga aagcggtctt cagcgcggcc ttccagcggg cggtcacgga
     1921 ggcggagtac ctgtccccga gcggctggtt caagctccac tacgacacga cgggagcgga
     1981 cgcggtcccg cccgaggatg cggacatgac cggagttccg gatctcgttg agaagtgcgc
     2041 agcttatctc gacagctcgc ggacccggca cctggactac ggctaccgga tgcccccgcc
     2101 ggacagcggg ctcggcggcg atgacctgtt cgatgtctac ttcgaggaga cgggggcgta
     2161 tggctacgcg atgccggagg gtcccggtcc ggagccctgg aacgacgtct acagctacct
     2221 cgtgctcaat aacgactttc tcggtttcgc tcccaactat gatccggagg gggatcaact
     2281 gggggcggcc aaagcgacct gcgcgcacga attccaccac tgcgtgcagt ttgcgtatga
     2341 cagcaacgag gactcctggt tcatggaaat ggacgccacg cacatcgagg acatcgtctt
     2401 tgacgccacc
//
LOCUS       DAOOKS010000282         2401 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_54712_length_2401_cov_3.323103, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000282 DAOOKS010000000
VERSION     DAOOKS010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54712_length_2401_cov_3.323103"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="PK112_05315"
     CDS             complement(<1..564)
                     /locus_tag="PK112_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPC11502.1"
                     /translation="MARERIVSGEQIIPDEDKALVTLRPSRLSEYIGQNKLRNKLKVT
                     LDAARGRGEAVEHMLFYGPPGLGKTTLAYIIANEMGTKLVATAGPALQRAGDLMGILT
                     NLNEGDILFIDEIHRLTPVIEEFIYPAMEDFKVDFVVDKGAFAKVINVPLKPFTLIGA
                     TTRAGFLSAPLRDRFGLYYHLDFYPPEE"
     gene            complement(564..1211)
                     /locus_tag="PK112_05320"
     CDS             complement(564..1211)
                     /locus_tag="PK112_05320"
                     /inference="COORDINATES: protein motif:HMM:NF025873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HPC11503.1"
                     /translation="MISRISGRLVNIENDTALVETGGLAYEIMLPSALAERLRDDGKV
                     GGDISLETLYYIDAGERKSAHFPRLVGFVNPIDREFFSVLTHVSGLGVKKVLKSLVMP
                     IRDIATAIENKDAAQLNRLPGIGARLANKIIAELHGKVAKFALSKEDQALSKAAEPVE
                     PLGEEARAVLLQLGYSRSEADRMVEGALKHGLKAARVEDLITVIFRNEQQKKAAE"
     gene            complement(1208..1699)
                     /gene="ruvC"
                     /locus_tag="PK112_05325"
     CDS             complement(1208..1699)
                     /gene="ruvC"
                     /locus_tag="PK112_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HPC11504.1"
                     /translation="MRVLGIDPGLQITGYGVIEDGGGAPVVLEAGVIRTTSGHDMSLR
                     LHELSCEIKGVIEQFRPDIIAVEELYSHYGHPRTAIIMGHARGIVFLKAAEAGVAVVP
                     YASTRIKKSLTGNGRASKRQMQMMIRSALHLPRPPEPPDAADALAVALCHCRAAAHHE
                     VAV"
     gene            complement(1791..2069)
                     /locus_tag="PK112_05330"
     CDS             complement(1791..2069)
                     /locus_tag="PK112_05330"
                     /inference="COORDINATES: protein motif:HMM:NF024639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HPC11505.1"
                     /translation="MTGLEYSSGKRATAIGKPEPLFFQAALEAIGVAPAEAIMVGDDI
                     ETDIGGAQRAGLRGVLVRTGKFREYLVRQSTVAPDLVIASLGDLPSHL"
     gene            complement(2106..>2401)
                     /locus_tag="PK112_05335"
     CDS             complement(2106..>2401)
                     /locus_tag="PK112_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR01458 family HAD-type hydrolase"
                     /protein_id="HPC11506.1"
                     /translation="LDREIRQRGYPIERDEIVAPTTLGAEWLRRKGRPSVFLVMREDT
                     QEEFAEFPRDDVRPDYIVIGNNRDRWDYALVNRIFLMLVNGSEMLALHKQRYW"
BASE COUNT          422 a          845 c          688 g          446 t
ORIGIN      
        1 ctcctccggc gggtagaaat cgagatggta gtacagcccg aaccggtcgc gcaggggcgc
       61 cgacagaaac cccgcccgcg tcgtcgcccc gatcagcgtg aacggtttca gcggcacgtt
      121 gatgaccttg gcgaacgccc ccttgtccac cacgaaatcg accttgaaat cctccatcgc
      181 cggatagatg aactcctcga tcaccggggt gaggcggtgg atttcgtcga tgaacaggat
      241 atccccctcg ttgagattgg tcagaatccc catgaggtcg ccggcccgct ggagcgccgg
      301 gccggcggtc gccaccagtt tggtccccat ctcgttggcg ataatgtacg ccagcgtagt
      361 cttgcccaac cccggcggcc catagaacag catgtgttcg accgcttccc cccgaccccg
      421 ggcggcatca agcgtgacct taagtttgtt tcgtaactta ttctgtccta tatactccga
      481 caagcggctc ggccgcagcg tcaccagcgc cttgtcctcg tcgggaataa tctgctcgcc
      541 tgacacgatc cgctcgcgcg ccatcactca gccgccttct tctgctgttc gttgcggaaa
      601 atcaccgtga tcaaatcctc gacccgcgcc gccttcagac cgtgcttgag cgccccttcc
      661 accatccggt cggcctcgct ccgcgagtac cccagttgga gcaggaccgc gcgggcctcc
      721 tcgccgagcg gctcgaccgg ttccgccgct ttggagagcg cctggtcctc cttggagagc
      781 gcaaacttcg ccaccttgcc gtgcagttcg gcgataatct tgttcgccag ccgcgcgccg
      841 atccccggca gccggttgag ttgggccgcg tctttgttct caatcgccgt cgcaatgtcg
      901 cggatcggca tgaccagcga cttcagaacc ttttttaccc ccaaaccgga tacatgggtc
      961 aaaaccgaga aaaactcccg gtcgatcggg ttgacgaaac cgaccaggcg ggggaaatgg
     1021 gccgacttcc gctccccggc atcgatatag tacagcgtct cgagactgat gtccccgccg
     1081 actttcccgt cgtcgcgcag ccgctcggcc agggccgacg gcagcatgat ttcgtaggcc
     1141 aggccccccg tctccaccag ggcggtgtcg ttctcgatgt tcacgagccg cccggagatg
     1201 cggctgatca tacggccacc tcgtggtgcg cggccgcccg gcagtggcac agcgccaccg
     1261 ccagcgcatc ggccgcatcg gggggttccg gtgggcgggg aaggtgcagg gccgaacgga
     1321 tcatcatctg catttgcctt ttgctcgccc ggccgttgcc cgtcagcgac ttcttgatgc
     1381 gcgtcgaggc gtacgggacc accgcgaccc cggcctcggc cgctttgagg aacacgattc
     1441 ctcgggcgtg ccccatgata atcgccgtgc gcgggtgacc gtagtgcgaa tacagctcct
     1501 caacagcgat gatatccggc cggaactgct cgatcacccc tttaatctcg cacgacaact
     1561 catgcaggcg caaggacata tcatgcccgc tcgtcgtccg gatcacaccg gcttcgagca
     1621 ccaccggcgc tcccccgccg tcctcgatca ccccgtaccc ggtgatctgc agtccgggat
     1681 cgatgcctag cacgcgcata ggctcaagta aggcaaagcc ggcccgaatt caacttaaat
     1741 ctggccgtgc ccaagtcaca ttcggcttat gcgcggcagc caccaccccg ttacagatgc
     1801 gatggcaggt cccccagcga ggcaatcacc aggtccgggg ccaccgttga ctgccgcacg
     1861 aggtactcgc ggaatttgcc cgtgcgcaca agcacacccc gcagcccggc ccgctgcgcc
     1921 cccccgatgt ccgtctcgat atcatcaccg accatgatcg cttccgccgg cgccaccccg
     1981 atcgcctcga gcgccgcctg gaaaaatagc ggctcgggct tgccgatcgc ggtcgcgcgc
     2041 ttcccgctgg agtattccag cccggtcacg aaacagccga tatcgaccgc gatgtcgtcc
     2101 ccgctctacc agtaacgctg cttgtgcagg gcgagcatct cggagccgtt gacgagcatg
     2161 agaaaaatcc ggttcaccag ggcatagtcc caccggtcgc ggttgttgcc gatcacgatg
     2221 tagtcgggcc gcacatcgtc gcgcgggaac tcggcgaatt cctcctgcgt gtcctcgcgc
     2281 atgaccagga agacgctggg gcgtcctttc ctgcgaagcc actcggcacc cagcgtggtc
     2341 ggcgcgacaa tctcgtcccg ctcaatcggg tagccccgct ggcggatttc ccggtcgagc
     2401 g
//
LOCUS       DAOOKS010000283         2393 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_54961_length_2393_cov_3.176219, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000283 DAOOKS010000000
VERSION     DAOOKS010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54961_length_2393_cov_3.176219"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            228..318
                     /locus_tag="PK112_05340"
     tRNA            228..318
                     /locus_tag="PK112_05340"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:262..264,aa:Ser,seq:gct)
     gene            535..>2393
                     /locus_tag="PK112_05345"
     CDS             535..>2393
                     /locus_tag="PK112_05345"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HPC11507.1"
                     /translation="MKLSYRAARFSHRLVLLAATSLLATTAPTVRAERASSSEMSRAC
                     ENWLTQVVFTRGAWAGAAAPTVLGAHELAVGDTLLARYYDISPRGFVLVAALKEMTPV
                     KAYSDRYNLDDSQSGGFLAMITEQLANRTRLYAEMFGSLEAAQPPEGPALFGRGQKAE
                     WTRLTLPAGEFGADLAAQAARATRDGGPLCTTSWDQGYPYNKFCPQGQYGTSVVGCVA
                     TAASQVIAYWQWPPSGVGSHTYYWDGDQYCGGNYGGGYLTASYSDSYDWANIVDSCSL
                     GCTPAQEDAVAELCYEVGVAFDMDYSSCGSAAYTGQAVLIYPQYFKFSPDVRRVDRSA
                     YSLHEWFDLVKNEIDSGRVVQYRINLHSIVLDGYREQIPGRYEYHMNYGWADAFNAWY
                     VFDSLYCGWIEGDICPASEEYMITHIFPQTQPVLQKVGEEIIEAGGDGNGRPNPGEAV
                     DLNIQVVNNGWDAVNPAVTLSTDDPYIAIEAASASLGDTLAWGETGFTQTPLKLAVDA
                     GCPDPHVAVLHVTMAAEGGHTASDSIRIFIGNSAGLASDFEVDDNRWWHRPHTQFFGD
                     QWHRETYRKHSGATSWKVGGTGGNYYDNALDAALTATAGDWLRVGWLGLTGYYL"
BASE COUNT          410 a          804 c          757 g          422 t
ORIGIN      
        1 cgccacgccc gatcccagga ctgacccgtg attgcagagc cgaccggcaa tggcggaacc
       61 gatctccggt ggcgtcccga aggcgtgacc gtacgacagg ttcccagcgg agggccgggc
      121 gccgactggc cagtgtgtgg cggggagtgc cggggcggca gaaaaaatgg cttgatcggg
      181 gggcggggcg ccgatatatt cctatgtacg aatccgcaat cctgaacgga gagatggccg
      241 agaggctgaa ggcggcggat tgctaatccg ttatagggct taatggctct atccagggtt
      301 cgaatccctg tctctccgct ttctaattgt caacgacact tgatttgaca cgtcacctcc
      361 ctccaggcag tgtggtttgc gtggtcagtg tcaccgggcc tcggcccgca gttggctctt
      421 cccgcgactc tcggttcgtc atgtttttcc tccattttcc ccaaaacctg ctatcttgcg
      481 gtatatgaca ctcggcgggc cgccgctgat aactcgattt cgtggggtat ggacatgaag
      541 ctctcttacc gcgccgctcg tttttctcac cgcctggttc ttctcgccgc cacctcgctc
      601 cttgccacaa ccgccccgac cgtgcgcgcc gaacgcgcct cgtcgtcgga gatgtcgcgc
      661 gcgtgcgaaa actggctgac ccaagtggtt ttcacccggg gcgcgtgggc cggcgcggcg
      721 gcgccgaccg tgctcggcgc ccacgagctc gcggtcggcg acaccctgct ggcccggtac
      781 tacgacatca gcccgcgcgg tttcgtgctg gtcgcggcgc tcaaagagat gacgccggtg
      841 aaagcgtatt ccgaccggta caacctcgat gactcgcagt cgggcggatt tctcgccatg
      901 atcaccgaac aactggcgaa ccgcacgcgc ctctatgcgg agatgttcgg cagcctcgag
      961 gcggcgcagc cgcccgaggg ccccgccctg ttcgggcgcg gccagaaagc cgagtggacc
     1021 cgcctcaccc ttccggctgg cgaattcggg gccgacctgg cggctcaggc ggcccgggcg
     1081 acccgcgacg gcgggcccct gtgcaccacc agctgggacc aggggtatcc ctacaacaaa
     1141 ttctgcccgc agggccagta cggcacctct gtggtcggct gcgtggccac cgcggcctcg
     1201 caggtgatcg cctactggca gtggccgccg agcggcgttg gatcccacac atactactgg
     1261 gacggcgacc agtattgcgg cggcaattac ggcggcggct acctcaccgc ctcctattcc
     1321 gattcctacg actgggcgaa catcgtcgac agctgcagcc tcggctgcac cccggcccag
     1381 gaagacgccg tggccgaact ctgctacgag gtcggcgtcg cctttgacat ggactactcc
     1441 tcctgcggtt cggcggccta caccgggcag gccgtgctca tctacccgca gtatttcaag
     1501 ttcagccccg atgtccggcg cgtcgaccgc agcgcctaca gtctccacga atggttcgac
     1561 ctggtgaaga acgagatcga cagcgggcgg gtcgtccagt accgcatcaa cctgcattcg
     1621 atcgtcctcg acggctaccg cgagcagatc ccgggccgct atgagtacca catgaactac
     1681 ggctgggcgg atgccttcaa cgcctggtac gtgttcgaca gcctgtactg cggctggatc
     1741 gagggggaca tctgcccggc cagcgaggag tacatgatca cccacatctt tccgcagacc
     1801 cagccggtgc ttcagaaagt cggcgaggag atcatcgagg cgggcggcga cggcaacggg
     1861 cgtcccaacc cgggcgaggc cgtggacctg aacatccagg tggtcaacaa cggctgggat
     1921 gcggtcaatc cggccgtcac tctttccacc gacgacccgt atatcgcgat cgaggcggca
     1981 tcggcgagcc tcggcgacac gcttgcctgg ggcgagacgg gattcaccca gacgccgctc
     2041 aaactcgctg tcgatgccgg ctgccccgac ccgcacgtgg ctgtcctgca cgtcaccatg
     2101 gcggccgagg gcggccacac ggcctccgac tcgatccgca tcttcatcgg caacagcgcc
     2161 ggactggcgt ccgatttcga ggtcgatgac aaccggtggt ggcaccggcc ccacacacag
     2221 ttcttcggcg accagtggca ccgcgagacc taccggaagc actccggcgc gacgagctgg
     2281 aaagtcggcg ggacgggcgg caactattac gacaacgccc tggatgcggc gctgacggcg
     2341 acggcgggcg actggctgcg cgtcggctgg cttggtctca cgggctatta cct
//
LOCUS       DAOOKS010000284         2393 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_54975_length_2393_cov_2.407186, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000284 DAOOKS010000000
VERSION     DAOOKS010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54975_length_2393_cov_2.407186"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="PK112_05350"
     CDS             complement(<1..594)
                     /locus_tag="PK112_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HPC11508.1"
                     /translation="MAIFINQKSRVLVQGITGRDGSFHAQQMKAYGTQVVGGVTPGKG
                     GSQVAGIPVFNTVEEAVGATGADVSVVYVPPAFAVDAIYEAVDAGIGLVVCITEGLPA
                     NDMMKVYPYVKARGARLIGPNCPGLISVDQCKIGIMPATIVKKGNVGVVSRSGTLTYE
                     AIWALTLAGMGQTTCIGIGGDQVIGTTFIDCLEAFEAD"
     gene            complement(607..1746)
                     /gene="sucC"
                     /locus_tag="PK112_05355"
     CDS             complement(607..1746)
                     /gene="sucC"
                     /locus_tag="PK112_05355"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HPC11509.1"
                     /translation="MKIHEYQAKQLFARHGIPVPAGDVATTPLEAYQIADRVNKPVMV
                     KAQIHAGGRGKAGGVKYAENAEAAKVLATTILGMDIKGLTVKKVLVTEAADIISEAYV
                     GIILDRASRRPVIMVSAAGGIDIEEVAAKTPEKIHKLAVDPVTGLKLFQARNLAYKLY
                     RDISQVRQAADIIMKLYETFWAVDASLVEINPLITNAASEVVAIDAKVNIDDNALYRQ
                     PAITAMRDLDAEPPTETQAREADLSYVKLEGTIGCMVNGAGLAMATMDLVKRYGGEPA
                     NFLDIGGSSNPQKVVTALRIITSDPNVKAILVNIFGGITRCDDVANGLVQALDELKPK
                     TPIVVRLAGTNEAEAARILARYHLPFEDTLDKVVKKAIALAGGQD"
     gene            complement(1743..>2393)
                     /locus_tag="PK112_05360"
     CDS             complement(1743..>2393)
                     /locus_tag="PK112_05360"
                     /inference="COORDINATES: protein motif:HMM:NF025112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HPC11510.1"
                     /translation="AYDVFEHTLRTIDACPRGLAVRLAALFHDINKPQAKREVIREGQ
                     PGATFYGHEMLGAATARSVLRRLRFAHDLIRQVTTLVERHMFTTDVTDKGLRRLVRRV
                     GVPLIFDLLDLRRADVTAQGMGGRTDDVDEFEARIRAELDRKPPFSLNDLALDGGDVM
                     RILKVAPGPLIGRVLDYLLEQVLDDPELNQPETLADLAREFYEATKESPNDKESDL"
BASE COUNT          355 a          872 c          705 g          461 t
ORIGIN      
        1 gtcggcctcg aacgcctcca ggcagtcgat gaaagtggtc ccgatcacct ggtccccgcc
       61 gattccgatg caggtggtct gccccattcc cgccagcgtg agcgcccaga tcgcctcgta
      121 ggtgagcgtc cccgaccgcg agaccactcc gacgttgccc tttttcacaa tcgtcgccgg
      181 catgatcccg atcttgcact ggtcgaccga gatgaggccg gggcagttgg gcccgatcag
      241 ccgcgccccg cgcgccttga cgtacgggta caccttcatc atgtcgttcg ccggaagtcc
      301 ttcggtgatg cacaccacca gcccgatccc ggcgtcgacc gcctcgtaga tcgcatcgac
      361 ggcgaacgcc ggcggcacgt agacgaccga cacgtccgcc ccggtcgccc cgaccgcctc
      421 ttccaccgtg ttgaacacgg gtattcccgc cacctgggag ccgccctttc cgggcgtcac
      481 gccgcccacc acctgcgtgc cgtaagcctt catctgctgg gcgtgaaacg agccgtcgcg
      541 gccggtgatt ccctgcacga gcactctgga tttctgattg atgaatatcg ccatacgcta
      601 cctcgcctag tcctgtccgc ccgccagcgc aatcgctttt ttgaccacct tgtcgagcgt
      661 atcttcaaac ggcagatggt agcgcgcgag gatgcgcgcc gcctccgcct cgttcgttcc
      721 ggccagccgc actacgatcg gcgtcttcgg cttcaactcg tcgagcgcct gcacgagtcc
      781 gttggccacg tcgtcgcacc gcgtgatccc cccgaagatg ttgaccagga tggccttcac
      841 gttgggatcc gaggtgatga tgcgcagcgc cgtcaccacc ttctgcgggt tggacgagcc
      901 gccgatatcg aggaaattgg ccggctcgcc cccgtagcgc ttcacgagat ccatggtcgc
      961 catcgccagc cccgctccgt tgaccatgca cccgatcgtc ccctcgagtt tcacgtacga
     1021 gaggtcggcc tcgcgcgcct gggtctcggt cggcggctcc gcgtccaggt cccgcatggc
     1081 cgtgattgcc ggctggcggt agagggcgtt gtcgtcgatg ttgactttcg cgtcgatcgc
     1141 caccacctcg ctcgccgcgt tcgtgatgag cggattgatt tccaccagcg acgcatccac
     1201 cgcccagaac gtctcgtaga gcttcatgat gatgtcggcc gcctggcgga cctggctgat
     1261 gtcccggtag agcttgtagg cgaggttccg cgcctggaac agcttcagcc cggtgaccgg
     1321 atcgaccgcc agcttgtgaa tcttctcggg cgtcttcgcc gccacttcct cgatgtcgat
     1381 cccgcccgcc gccgacacca tgatcaccgg cctccggctc gcccggtcga ggatgattcc
     1441 cacgtacgct tccgagatga tgtcggcggc ctccgtaacc agcaccttct tgaccgtcag
     1501 acccttgatg tccatcccca ggatggtggt ggcgagcact ttcgcggcct cggcgttttc
     1561 ggcgtatttg acccccccgg ccttgccgcg gccgccggcg tggatctgcg ccttcaccat
     1621 caccggcttg ttcacccggt cggcgatctg atacgcctcc agcggcgtcg tcgccacgtc
     1681 ccccgccggc accgggatcc cgtggcgcgc gaacagttgc ttcgcctgat actcgtgaat
     1741 cttcataggt cggattcctt atcgttcgga ctctccttcg ttgcctcgta gaactcccgg
     1801 gccaggtccg ccagcgtctc cggctgattc agctccgggt cgtcgagcac ctgctccagc
     1861 aggtagtcca gcacgcggcc gatcagcggc ccgggcgcca ccttcaggat acgcatcacg
     1921 tcgccgccgt caagtgccag atcgttcagc gaaaacggcg gtttccggtc cagctccgcg
     1981 cggatccggg cctcaaattc gtccacatcg tccgtccgtc cccccatgcc ctgggcggtc
     2041 acatcggccc gccgcaggtc caaaaggtcg aaaataaggg gcacgcctac ccggcggacc
     2101 agccggcgca accccttgtc ggtcacgtcc gtcgtgaaca tgtgccgctc gaccagggtg
     2161 gtcacctgcc ggatcaggtc gtgggcgaag cgcaggcggc gcagcacgct ccgggcggtc
     2221 gccgcgccga gcatctcgtg gccgtagaac gtcgcccccg gctgcccctc ccggatcacc
     2281 tcgcgcttgg cctgcggctt gttgatatcg tggaacagcg ccgccagccg caccgccagc
     2341 ccgcgcgggc aggcgtcgat cgtccgcagc gtgtgttcga acacgtcgta ggc
//
LOCUS       DAOOKS010000285         2390 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_55060_length_2390_cov_2.586296, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000285 DAOOKS010000000
VERSION     DAOOKS010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55060_length_2390_cov_2.586296"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="PK112_05365"
     CDS             complement(<1..373)
                     /locus_tag="PK112_05365"
                     /inference="COORDINATES: protein motif:HMM:NF015331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HPC11511.1"
                     /translation="MRLHTLQREQFVPRPRDEVFAFFSRPENLPRLTPPALRFRILTP
                     PPLDMMPGALIDYTLRTFGFTVRWTTLIAACDPPARFVDVQLRGPYAFWHHAHEFVAA
                     EGGTRIIDRVTYALPLGPLGQL"
     gene            complement(370..1746)
                     /locus_tag="PK112_05370"
     CDS             complement(370..1746)
                     /locus_tag="PK112_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020885580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HPC11512.1"
                     /translation="MAAPVDPRRVRVVRPGELSDGPIVYWMSRDQRAADNWALLYAGE
                     IALRRHRPLAVVFCLAPAYLDAALRQYAFMLRGLEETAADLRRLAIPFFLLAGAPPDR
                     LRAFVRRHKVGLVVADCSPLRINRRWLEEFAAASPCPVHQVDAHNIVPVWEASDKQEY
                     AAYTLRPKIRRRLPEFLTDFPRLRRHPFAWPGEPPPVDWAAARRSLRVDRSVPEVDWL
                     APGPRAAFRLLRAFFDRRLHLYADHGHDPNVEAHSDLSPYLHFGQIAPQRVAWNAQPF
                     DRSIPALEAFLEQLIVRRELSDNFCWYNSAYDSCAAFPEWARITLEEHRRDPRPRLYS
                     RDQLEAAQTDDPLWNAAQRDLVIGGKMHSYLRMYWAKKILEWSPSPEEALAAAVALND
                     RYELDGRDPNGYAGIAWCIGGVHDRAFGARDVYGKIRCLSAAGARRKFDVAAYIRAGN
                     RRTAGRNE"
     gene            complement(1747..>2390)
                     /locus_tag="PK112_05375"
     CDS             complement(1747..>2390)
                     /locus_tag="PK112_05375"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HPC11513.1"
                     /translation="FVRLALEAVADLDARRLESVLLQASVALGRIVLLQEVVMPLLDR
                     VGTEWSGGRLKIAHEHLASSTLRSFLGHLAETAAAEQDAPLLLTTTPAGQVHEFGALV
                     STVAAASVGWRTAFLGPNLPAEDIAGAARQTSARAVALSIIYPPDDARLADELRTLRR
                     LLDPAVRIIVGGRSKDGYRRTLAEIGALAVDNLPGLIEGLDSLRAASPSDGER"
BASE COUNT          391 a          684 c          995 g          320 t
ORIGIN      
        1 cgagctgccc gagcggtccc aggggcaggg cgtaggtgac gcggtcgatg atccgggtgc
       61 cgccctcggc ggccacgaac tcgtgggcgt ggtgccagaa cgcatacggt ccccggagct
      121 gaacatcgac gaaccgggcg ggggggtcgc aggcggcgat cagggtggtc cagcgcactg
      181 tgaagccgaa ggtgcgcagg gtgtagtcga tgagggctcc gggcatcatg tcgagcggcg
      241 ggggagtgag aatccgaaag cgcagcgcgg gcggcgtgag gcgcgggagg ttctcggggc
      301 gggagaagaa ggcgaagact tcgtcgcggg ggcgggggac gaactgctcg cgctgaagcg
      361 tgtgcagcct cattcattcc tcccggcggt gcggcggttg ccggcgcgga tgtaagcggc
      421 gacatcgaac ttgcgccgcg cgccggcggc gctcaggcag cggatcttgc cgtagacgtc
      481 gcgggcgccg aaggcgcggt cgtgcacgcc gccgatgcac caggcgatgc cggcgtaacc
      541 gttggggtcg cggccgtcga gttcgtagcg gtcgttcagg gcgacggcgg cggcgagcgc
      601 ctcttcgggg gacgggctcc actcgagaat cttcttggcc cagtacatcc ggagatagct
      661 gtgcattttc cccccgatga ccaggtcgcg ctgggcggcg ttccagaggg gatcatcggt
      721 ctgcgcggcc tcgagctggt cccgggagta gaggcgggga cggggatcgc gacggtgttc
      781 ctcaagcgtg atccgggccc actcggggaa agccgcgcac gaatcatagg cggagttgta
      841 ccagcagaag ttgtcggaga gctcgcgacg cacgatgagc tgttcgagga acgcctccag
      901 ggcggggatg gagcggtcga agggctgggc gttccaggcg acgcgctgag gggcgatctg
      961 gccgaagtgg aggtatgggg agaggtcgga atgggcttcg acgttggggt cgtggccgtg
     1021 atcggcgtac aggtggagac ggcggtcgaa gaaggcgcgc aggaggcgga aggcggcccg
     1081 ggggccgggg gcgagccagt cgacctcggg gacggatcga tcgacgcgca gagagcggcg
     1141 ggcggcggcc cagtcgaccg ggggaggttc gccgggccag gcgaagggat ggcggcgcag
     1201 gcgcgggaaa tcggtgagaa actccggcag ccggcggcgg attttggggc ggagcgtgta
     1261 cgcggcgtac tcttgtttgt cggaggcctc ccacaccggc acgatgttgt gggcgtcgac
     1321 ctgatgcacc gggcaggggg aggcggccgc gaattcctcg agccaccgcc ggttgatccg
     1381 gaggggagag cagtcggcga cgaccaggcc gaccttgtgg cggcggacaa acgcgcgcag
     1441 ccggtcgggc ggagcgccgg ccaggaggaa gaaggggatg gcgaggcggc ggaggtcggc
     1501 ggccgtttct tcgaggccgc ggagcatgaa tgcatactgg cggagcgcgg cgtcgagata
     1561 ggcgggggcg aggcagaaga cgacggcgag cggccggtgg cggcgcagcg cgatctcccc
     1621 ggcgtagagg agagcccagt tgtcggcggc gcgctggtcg cggctcatcc agtagacgat
     1681 gggtccgtcc gacagctcgc ccgggcggac gacgcggaca cgccgcggat cgaccggggc
     1741 ggccactcag cgctccccat cggaggggga cgctgcccgc aaggaatcga ggccctcgat
     1801 caaaccgggg aggttgtcga cggcgagggc gccgatctcg gcgagcgttc tccggtagcc
     1861 gtccttcgag cgtccgccga caatgatccg gaccgcgggg tcaagcaggc ggcgcagggt
     1921 gcgcagttca tcggcgaggc gggcatcatc gggcgggtag atgatgctga gggcgacggc
     1981 ccgcgcggag gtctggcggg cggcgccggc gatatcttcg gccgggagat tcggtccgag
     2041 gaaggcggtg cgccagccga ccgaggcggc ggccaccgta ctcactaaag cgccgaattc
     2101 gtggacctgc ccggccgggg tggtggtgag cagcagcggg gcatcctgct cggcggcggc
     2161 tgtctccgcg agatggccga gaaaggagcg gagggtggag ctggcgaggt gctcgtgggc
     2221 gatcttgagg cggccgccgc tccattcggt ccccacgcgg tccagcagcg gcatgacgac
     2281 ctcctgaagc aggacgattc gcccgagggc gaccgaagcc tgcagaagaa ccgactccag
     2341 ccggcgggca tcaaggtccg cgacggcttc gagagcgagt ctcacaaagt
//
LOCUS       DAOOKS010000286         2383 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_55282_length_2383_cov_3.549828, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000286 DAOOKS010000000
VERSION     DAOOKS010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55282_length_2383_cov_3.549828"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="PK112_05380"
     CDS             complement(<1..313)
                     /locus_tag="PK112_05380"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HPC11514.1"
                     /translation="MNSKAAPECSVRKGGSHWTISFSAMASPCEILIRCRRRSEAEHV
                     ASLAAAETRRIERKLSRYRDDNIIHAINAGTGAAVEVDDELARLLTYADQCYRLSGGL
                     FD"
     gene            complement(303..1652)
                     /locus_tag="PK112_05385"
     CDS             complement(303..1652)
                     /locus_tag="PK112_05385"
                     /inference="COORDINATES: protein motif:HMM:NF023516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3570 domain-containing protein"
                     /protein_id="HPC11515.1"
                     /translation="MRMQLTNRPPLRGALSALTAALLGTGAAASEGRNRVESSILLYS
                     EVDRVQAAEAIIGLGRALKGDRMLSARLTLDGLTGASPNGATPSARVQTFTRPSGEGG
                     YSVKPGEIPLDDTFKDTRYSFDGSLAQPLDRLTTIVLGAHYSGEHDYTSLGASVGLSR
                     DLNRKNTTLSASAAYSRDRVRPEGGPPDPLSSMSARADDDDDDDAGERELAAQDDDGD
                     DDDDEDRESDAGEGKNVYDVVFGLTQVLNRQTVLRLNYSYNHSSGYLNDPYKIVSVVQ
                     GPGGPDAGEPVDYLYEARPDGRAKQAVFAQLRRYLSGHTIDLSYRYFWDDWGVTSQTV
                     ELFYRLPVKAGHALQPHVRWYRQTEADFYRVFLVEAAPAPAHASADYRLAPFHAVTAG
                     LQYLFPVGQGVQVSLGGEYYHQAGDISPPRSMGVLSTYELFPDMDAVMVRAGFDYEF"
     gene            complement(1637..1870)
                     /locus_tag="PK112_05390"
     CDS             complement(1637..1870)
                     /locus_tag="PK112_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012800199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4266 domain-containing protein"
                     /protein_id="HPC11516.1"
                     /translation="MRRLRFFVLSAGAFALAAVLSGCAAVGVQVWERDVLSKPEMQMD
                     SHALDRALDDHIYFSKEASSGGRSFGGGGCGCN"
     gene            complement(1867..2373)
                     /locus_tag="PK112_05395"
     CDS             complement(1867..2373)
                     /locus_tag="PK112_05395"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPC11517.1"
                     /translation="MIAFVRPRGVGVLLAALVLSAAAVPCLAAPPDSLNALQTALADS
                     LDLNGKVVYVDFWASWCVPCRHSFPWMQALSDKFRNRGLEIIAVGVDQDHRAAVKFLE
                     ENRTAFPIVFDSTGRLAKLYELEAMPTSFLYDRSGRLVSTHRGFRPDETDSLEAAIVR
                     LLDKGGAQ"
BASE COUNT          406 a          746 c          855 g          376 t
ORIGIN      
        1 tgtcgaagag gccgccgctg aggcggtagc actggtcggc ataggtgagg aggcgcgcga
       61 gctcgtcatc gacttccacc gccgcgccgg tgccggcgtt gatggcgtgg attatgttgt
      121 cgtcgcggta ccggctgagt ttccgttcga tgcggcgcgt ttccgcggcc gcgagcgaag
      181 ccacgtgttc ggcttcgctc cggcggcggc agcgaatgag aatctcgcag ggactcgcca
      241 tggcggagaa ggaaatcgtc cagtgggatc cgcccttccg gacagagcac tccggcgcgg
      301 ccttagaatt catagtcgaa tccggcccgg accatgacgg cgtccatgtc ggggaacaac
      361 tcgtacgtgc tcagcacgcc catcgaacgg ggcgggctga tatcccccgc ctggtgatag
      421 tactcgccgc cgaggctcac ctgaaccccc tgaccgaccg ggaacaggta ctgcagcccg
      481 gccgtcaccg cgtgaaacgg agccagccgg tagtcggccg aggcatgggc cggcgccgga
      541 gcggcctcga ccaggaaaac gcggtagaag tccgcctccg tctggcggta ccagcggacg
      601 tggggctgga gcgcgtgccc ggctttcacg ggcagtcggt agaaaagttc aacggtctgg
      661 gaggtcacgc cccagtcgtc ccagaagtag cggtaggaga ggtcgatcgt gtgcccgctc
      721 aggtagcggc gcagttgggc gaaaaccgcc tgcttggcgc ggccgtcggg acgggcctca
      781 tagaggtaat cgaccggttc gccggcgtcg ggaccacccg gtccctgcac aacgctcact
      841 atcttgtagg gatcgttcag gtagccggac gagtggttgt aggagtagtt gagacggagg
      901 acggtctgtc gattaagcac ctgcgtgagg ccgaagacga cgtcgtacac gttcttcccc
      961 tcgccggcgt cgctctcacg gtcctcgtcg tcatcgtcgt cgccgtcgtc atcctgagcg
     1021 gcgagctccc gctcgcctgc gtcgtcgtca tcatcgtcgt cggcccgcgc gctcatcgaa
     1081 ctcagcgggt caggaggacc gccttcgggg cggacccggt cgcgcgaata ggcggccgag
     1141 gcggacagcg tcgtgttctt ccgattcagg tcgcgggaga gaccgacact ggcccccagg
     1201 gatgtgtagt catgctcgcc cgagtagtgg gcgccgagga caatggtggt gaggcggtcg
     1261 agcggctgag ccagactgcc gtcgaagctg tagcgcgtgt ctttgaaggt gtcgtcgagc
     1321 ggaatctcgc ccggctttac ggaataaccg ccctcgccgg aggggcgggt gaaggtttgc
     1381 acgcgggccg acggcgtggc gccgttgggc gaggcgccgg tgaggccgtc gagggtcagc
     1441 cgcgcgctga gcatccggtc ccccttgagc gcccggccca ggccgatgat cgcttcggcc
     1501 gcctgcacgc ggtcgacctc ggaatacagg aggatcgacg attccacgcg attccgcccc
     1561 tcggacgcgg ccgcgccggt cccgagcagg gccgcggtga gggcgctgag cgcgcctcgg
     1621 agaggcggcc ggttggtcaa ttgcatccgc atcccccgcc gccgaagctg cgcccgccgc
     1681 tggaagcctc cttgctgaaa tagatgtggt catccagggc gcggtcgagc gcatggctgt
     1741 ccatctgcat ttccggtttg gagagaacgt cgcgttccca gacctgcacg ccgacggcgg
     1801 cgcacccgga gaggacggcc gcgagcgcga aagccccggc cgaaagcacg aagaatcgaa
     1861 gccgtctcac tgcgcccctc ccttgtcgag caggcggaca atggccgcct cgagactgtc
     1921 ggtctcatcc gggcggaagc cccggtgagt ggagacaagg cggccgctcc ggtcatagag
     1981 gaaggaggtg ggcatcgcct ccagttcata gagcttggcc agacggccgg tcgaatcgaa
     2041 gacgatcggg aaagcggtgc ggttctcctc gaggaacttc accgccgcgc ggtggtcctg
     2101 gtcgacgccg acggcgatga tctcgagacc gcggttgcgg aacttgtcgg agagggcctg
     2161 catccagggg aaagagtggc ggcagggcac gcaccacgag gcccagaagt cgacatacac
     2221 gaccttgccg ttcaggtcga gcgaatccgc cagagcggtc tgcagtgcgt tgagggagtc
     2281 gggcggggcg gccagacagg ggacggcggc ggccgacagc accagggcgg cgagcaacac
     2341 gcccactccc cgcgggcgga cgaaggcgat catgaatcag cgg
//
LOCUS       DAOOKS010000287         2377 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_55468_length_2377_cov_3.382860, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000287 DAOOKS010000000
VERSION     DAOOKS010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55468_length_2377_cov_3.382860"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1272
                     /locus_tag="PK112_05400"
     CDS             <1..1272
                     /locus_tag="PK112_05400"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HPC11518.1"
                     /translation="DTVEELFDVAKALSMQPVPQGNRVAVVTNAGGPGILATDALINN
                     GMEMPPLSPETVKRLRQFSSEDASFANPMDMVAGAGPEQFRRTLGAIITDKRYDSIIP
                     IFVPPVTIDELEVARSIQEAVAGTKKTVLACFMGASRNSAGIEHLRSHGIPVYIYPEA
                     IAKTLATVSHYRRRMNAPRGKVRTFEVDPDRVQAVIERARTESRTTIVGAEAIEILCA
                     YGIPAAEYRFASSEREAAAIAKGMGLPVVMKVAAPAILHKTEVGGVMLDLRTEREVRD
                     AFRELRKRVAAERAAGETFAVALQQMVAGGVETVMGMTTDPSFGPLIMFGLGGIYVEI
                     MKDVAFRINPLTTADVHDMMRSLKSYPLLKGYRGAPPVDLATVEDTLLKVSQLVKDFD
                     AFSEVDINPFIASPERARCKAVDARFIMKEG"
     gene            1382..1954
                     /locus_tag="PK112_05405"
     CDS             1382..1954
                     /locus_tag="PK112_05405"
                     /inference="COORDINATES: protein motif:HMM:NF023511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11519.1"
                     /translation="MKLELKAVSVWTAVKIGFFLNLIMGFIMGLVAALFMAPLMAVMS
                     TMMPAGAGGSEFAAMPVGLLLVILPIGYAIFGAVFGTITFAIGAGVYNVTARILGGLE
                     ITYTVVEWGDPSGAAQAPPPFPRYPAPAPPYPAYPPPTGRPGPYSGSPMPPPPPPPEA
                     RPEPQAPHRPLPEAPPPGGPPEEPEKPTQP"
     gene            1964..>2377
                     /locus_tag="PK112_05410"
     CDS             1964..>2377
                     /locus_tag="PK112_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HPC11520.1"
                     /translation="MTDRSIRVRIAPSPTGYLHVGTARTAIFNWLFARHSGGKFLVRI
                     EDTDQARSQADLIAPILDAMRWLGLQWDEAITYQSQRLDTYNACARRILELGRGYRCF
                     CTPAELERGREEAKARKQPLRYDRRISGRHAQFPTC"
BASE COUNT          397 a          783 c          833 g          364 t
ORIGIN      
        1 gacacggtgg aggagctgtt cgacgtggcg aaggcgctgt cgatgcagcc ggtgccgcag
       61 gggaaccggg tggcggtggt gaccaacgcc ggcggcccgg ggattctcgc caccgacgcc
      121 ctgatcaaca acgggatgga gatgccgccg ctgtcgcccg agacggtgaa gcggctgcgg
      181 cagttcagtt ccgaggacgc gtcgttcgcc aacccgatgg acatggtggc cggggccggt
      241 cccgagcagt tccgcaggac gctcggcgcc atcatcaccg acaagcgcta cgacagcatc
      301 atccccatct tcgtcccgcc ggtgacgatc gacgaactcg aggtggcgcg gagcatccag
      361 gaggcggtgg ccgggacgaa gaagacggtg ctcgcctgtt tcatgggggc cagccgcaat
      421 tcggccggca tcgaacacct ccgcagccac ggcatccccg tctacatcta ccccgaggcg
      481 atcgcgaaga cgctggcgac ggtgagccac taccgccggc gcatgaatgc cccccggggg
      541 aaagtccgga ccttcgaggt tgacccggac cgggtgcagg cggtcatcga gcgggcgcgc
      601 acggaaagcc gcacgacaat cgtgggtgcg gaggcgatcg agattttgtg cgcctacggc
      661 atcccggcgg ccgagtaccg cttcgccagc agcgagcgag aggcggccgc gattgcgaaa
      721 gggatggggc tgccggtggt gatgaaggtg gcggcgccgg cgattctcca caagactgag
      781 gtcggcggcg tgatgctcga cctgcgcacc gaacgagagg tgcgcgacgc gttccgcgaa
      841 ctccgcaaac gggtcgcggc cgagcgcgcc gcgggcgaga cgttcgcggt ggccctccag
      901 caaatggtgg cgggcggggt cgagacggtt atgggcatga ccaccgatcc ctcgttcggg
      961 cccctcatca tgttcggact gggcgggatc tacgtggaaa tcatgaaaga cgtcgccttc
     1021 cggatcaacc cgctcacgac cgcagatgtg cacgacatga tgcgctcgct gaagtcctac
     1081 ccgctcctga aagggtaccg tggggccccg ccggtcgatc tggcgacggt cgaggacacg
     1141 ctgctgaagg tgtcgcagct ggtgaaggat ttcgacgcct tcagcgaagt cgacatcaac
     1201 cccttcatcg cctcgccgga gcgcgcgcgg tgcaaggcgg tggatgcgcg gttcatcatg
     1261 aaagagggct gagggccgga ttatcgcttg aatttccgca ccggcggcgt atcttggcac
     1321 cacccccggc ggcgacccgc cgtccggggc ggagaagggt gggaacaccc gggaggacgc
     1381 gatgaaactg gagctgaaag cggtctcggt ctggacggcg gtcaagatcg gttttttcct
     1441 gaacctgatc atgggattca tcatgggcct ggtggcggcc ctctttatgg ccccgctcat
     1501 ggcggtgatg tcgacgatga tgccggcggg agccggcggc tcggagttcg cggccatgcc
     1561 ggtgggactc ctcctggtga ttctgccgat cgggtacgcg attttcgggg cggtgttcgg
     1621 gaccatcacc ttcgccatcg gcgcgggcgt gtacaatgtc accgcccgca ttctgggcgg
     1681 gctggaaatc acgtacacgg tggtggagtg gggggatccg agcggcgcgg cgcaagcgcc
     1741 gccgccgttc ccgcgctatc ccgcacccgc gccgccctat ccggcctatc cgccgcccac
     1801 cgggcggccg ggaccctact ccggctcgcc gatgccgccc ccgccgccgc ctccggaggc
     1861 gcgcccggag ccccaggcgc cgcaccggcc gctccctgag gcgcccccgc cgggcggacc
     1921 tcccgaggaa cccgagaaac cgacccagcc gtagatgccg gaaatgaccg atcgatccat
     1981 tcgcgtacgc atcgccccgt cgccgacggg ctacctccac gtggggacgg cccggacggc
     2041 cattttcaac tggctgtttg ctcggcactc cggcgggaag ttcctcgtgc gtatcgagga
     2101 caccgaccag gcgcggtcgc aggcggatct gatcgcgccc attctcgacg ccatgcgctg
     2161 gctgggactg caatgggacg aggcgatcac gtaccagtcg cagcggctcg acacctacaa
     2221 cgcctgcgcg cggcgcattc tcgagctggg ccgcgggtac cgctgtttct gcacgcccgc
     2281 ggaactcgag cggggacggg aggaggccaa ggcgcgcaag cagccgctcc gctacgaccg
     2341 gcgcatctcg ggacgtcacg cgcagtttcc aacatgc
//
LOCUS       DAOOKS010000288         2376 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_55518_length_2376_cov_2.573029, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000288 DAOOKS010000000
VERSION     DAOOKS010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55518_length_2376_cov_2.573029"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..892
                     /locus_tag="PK112_05415"
     CDS             <1..892
                     /locus_tag="PK112_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11521.1"
                     /translation="LGALGLFSGERAPVLDTGALPPAAAVAAYAALIVAGFALGSLPV
                     RRRAVLGGLVLLNGALAWLALGPRPPRVVCDIHITRVPGGVAAAIVPAGAERADLVIT
                     NLAARDYPVVDRVLIPWLERLGRPGLGRVVLLSADYAGAAELARFADSLRADTLFVHG
                     RLHGTMGDIAAWSGRCPPWRPFGGAEPPAGEGLALCAWGAVLAARGLEILFLRTDGDL
                     AAARCAPDRACWLVCGDEAAADDLRSIGRPPAGLVCAEFAQTEPDSVPAAVFDLRRFG
                     SVRLRLTAGAAPSVRVETGR"
     gene            1002..2204
                     /locus_tag="PK112_05420"
     CDS             1002..2204
                     /locus_tag="PK112_05420"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HPC11522.1"
                     /translation="MSLSGNLKTVSFPDILQLLATGKKTGLLEIRTASRQKEVAFRDG
                     NIIHASSVNSAEDLLGQMLLNRGRLSKKDLDRAITLHKQTGRQLGTTLIDMGVFDKHE
                     IAECLKQQIEEIVYNLFSWKEGEFLFHENAHPKSAPFLVELSTMSVIMEGTRRIDEWL
                     EIQKVLPPDDVLLAISRSPRTNEEEFKISVDEFRLIGLINGERTLPDLINLSPMGEFV
                     TCRAIYRLIVNHLIEAVGRREQKNQVEEDEEEAVLRIVFRLYNNAFYRIRTLFEEYLG
                     EDNTTFATFAAQYRGNLLNFFPGVDPKSDLMPSFDKFLAAVQAIPAPIRFQTLFRDLD
                     DALAEQLQFIFELLGVGAYRDGITTVKGEISEPLATRRDLVKRYAIEENFNQAVKRSA
                     RVVKMVRG"
BASE COUNT          378 a          846 c          737 g          415 t
ORIGIN      
        1 cctcggggcg ctgggcctgt tctcgggaga gcgcgcgccc gtcctcgaca ccggcgcgct
       61 gccgccggct gccgcggtcg ctgcctacgc cgccctgatt gtcgccggct tcgctctcgg
      121 atcgctcccg gtgcggcggc gggcggtgct tggcggcctg gtcctcctca acggcgcgct
      181 cgcctggctg gccctcggcc cccggccgcc ccgggtcgtc tgcgacatcc acatcacgcg
      241 cgtccccggc ggggtggccg ccgccatcgt cccggccggt gcggagcggg ccgatctggt
      301 catcaccaac ctcgccgccc gggactaccc ggtggtcgac cgcgtcctga tcccctggct
      361 ggaacgcctc ggtcggccgg gactcggccg ggtagtgctg ttgtccgccg attatgccgg
      421 cgcggccgag ctcgcgcgct tcgccgacag cctgcgcgcg gacaccctgt ttgtccacgg
      481 ccggctgcac ggcaccatgg gggacatcgc ggcatggtcc gggcgctgtc caccctggcg
      541 ccccttcggc ggggcggagc cgccggccgg cgagggcctg gcgctctgcg cctggggcgc
      601 ggtgctcgcc gcccggggcc tcgaaatcct gttccttcgg accgacggcg atctcgcggc
      661 cgcccgctgc gcccccgacc gcgcctgctg gctggtctgc ggcgatgaag cggccgcgga
      721 cgacctccgc tcgatcggcc gcccgcccgc gggtctggtc tgtgcagaat ttgcacagac
      781 cgaaccggac tccgtccccg ccgccgtctt tgacctgcgc cggttcgggt ccgtgcgcct
      841 gcggctgacg gccggcgccg ccccctcggt ccgcgtggaa accggccgct gaaccttttc
      901 ccgtcaagtt ccgattggcg gctcccgata catatagaga actggcgtac ccgcgctggc
      961 ggggcctacc atggtgtgga gagaaggaag aagggtcgca gatgagtcta tccggtaacc
     1021 tgaaaacggt atcattcccc gacatcctcc agcttctggc gacggggaag aagacgggac
     1081 tgctggaaat cagaaccgcc agcaggcaga aagaagttgc cttccgcgac ggcaacatca
     1141 ttcacgcctc gtcggtaaac tccgccgagg acctcctcgg tcagatgctg ttgaaccgcg
     1201 gccggctctc gaagaaggat ctcgaccggg ccatcacgct ccataagcag accggacggc
     1261 agctcggcac caccctcatc gacatgggcg tcttcgacaa gcacgagatc gccgagtgcc
     1321 tcaaacagca gatcgaagag attgtgtaca acctgttctc gtggaaagag ggcgagttcc
     1381 tgttccatga gaacgcccac cccaagagcg ccccgttcct cgtcgagctc agcacgatga
     1441 gcgtcatcat ggaggggacg cggcgcatcg acgagtggct cgagatccag aaagtgctcc
     1501 cgcccgacga cgtcctcctg gcgatctcgc ggtcgccccg gactaatgag gaggagttca
     1561 aaatctccgt cgacgagttc cggctcatcg gcctcatcaa cggcgagcgc accctccccg
     1621 acctcatcaa cctctcgccc atgggcgagt tcgtcacctg ccgcgccatc taccgcctta
     1681 ttgtcaatca cctcatcgaa gcggtcgggc ggcgcgagca gaagaaccag gtcgaggagg
     1741 acgaggaaga ggccgtgctc cgcatcgtct tccggctcta caacaatgcc ttctaccgca
     1801 tccgcacgct cttcgaagag tacctcggcg aggacaacac cactttcgcc accttcgccg
     1861 cccagtaccg cggcaacctg ctgaacttct tcccgggcgt cgaccccaag tccgacctca
     1921 tgccatcgtt cgacaagttc ctcgccgcgg tccaggccat tcccgcgccg attcggttcc
     1981 agacgttgtt ccgcgacctc gatgacgccc tggccgaaca gctccagttt atcttcgaac
     2041 tgctcggcgt cggggcctat cgcgacggca tcacgacggt caagggcgag atttccgagc
     2101 cgctggccac ccggcgcgac ttggtgaagc gatacgcgat cgaagaaaat ttcaatcagg
     2161 ccgtgaaacg gtctgcccgc gttgtcaaaa tggtgagagg gtgaatatga aacgcacgat
     2221 tatgtccgct ctgctgctgg ccggggcgtt ggtaccgttc tcgcgcgccg aggccgccaa
     2281 catcgccgcc attatcaacc cgccgacctt cctgaatctc ctggtcctgg tcggcgcggt
     2341 cgtgtgtacc gccggcgccg tgcaggtttt ctcgct
//
LOCUS       DAOOKS010000289         2369 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_55756_length_2369_cov_2.656871, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000289 DAOOKS010000000
VERSION     DAOOKS010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55756_length_2369_cov_2.656871"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1063)
                     /locus_tag="PK112_05425"
     CDS             complement(<1..1063)
                     /locus_tag="PK112_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11523.1"
                     /translation="MLAWIGIAPAAPQGPAPRIEVIYPKEGQTVAAVDSTFILGNIKP
                     CTHRDQWRLTINGAAVPVHDSGGFIAFLPIAPGPFTFRLEARPVGYDDAGRQHRQKTS
                     FPENEPSAPIACSITVAIPAPAVSYPRDVLRLGREYRPPAGDLYLAAGDRVEAWIQAT
                     PGCRAWFAIPGVADSVPMAETDPRFQPYWGEAVFGEGAVPESLRIAGIYSGFYDVPAA
                     ARADSVRLRYFLAPPGPAAQSHTALESASRAADSALLTLRLPDTVLTDSSTWRLSLNS
                     PVFPFTVRFSDSVKVIRYAPQRAYLAVGPPGGVEALAVGRAGDWYKLRLSRSQVAWVD
                     RRTVTPLPRGILPPQSFLRS"
     gene            complement(1086..2114)
                     /locus_tag="PK112_05430"
     CDS             complement(1086..2114)
                     /locus_tag="PK112_05430"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HPC11524.1"
                     /translation="MMQLDAVKVNLPLKKKFAVSQGEVTVKTNLLTILNNRYSGEAST
                     SIYYGPSIEEMAADLRKGIALLERQTAVTLGTLHDIDRMDIHPAARSALMAAVLNYLS
                     GESRRYPWELVDLGTPIGIKSSMTVGIDATDRMVEAIRSSEYPIIKVKMGHEQDVMIL
                     DALEAGLGKEVRVDANGAWSCAKAEEMIYHLSRKGVRVIEQPTDADYVKEWPHLKGKA
                     DVELIMDEGLNTLDDYMRCRASIDGINIKMDKCGGILHGVALARQAQADRKKVMLGCM
                     VESSVGIAQSVYMSALADYFDLDGPLLLEDDIARGISYHRESINVDREIIGGPKLRRD
                     VLQKYLSE"
     gene            complement(2111..>2369)
                     /locus_tag="PK112_05435"
     CDS             complement(2111..>2369)
                     /locus_tag="PK112_05435"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HPC11525.1"
                     /translation="GHRLGLSLPRDSKDQIRTGRPVERARTICGDLVFFPGHVAIAVG
                     RDRIIHSSRGGGGVRIESLAPDAPAYRSDLDQSYLEARRII"
BASE COUNT          401 a          716 c          864 g          388 t
ORIGIN      
        1 gcgagcgcag gaacgactgc ggggggagga tgccgcgcgg gagcggcgtc accgttcggc
       61 ggtccaccca ggcgacctgc gagcgggaga gccgcagctt gtaccagtct cccgcccggc
      121 cgaccgcgag cgcctccacc ccgcccggcg ggccgaccgc gaggtaggcg cgctgcgggg
      181 cgtagcggat gactttgacg gaatcggaaa agcgaacggt gaacgggaag accgggctgt
      241 tgagcgaaag ccgccaggtg ctggagtcgg tgaggacggt gtcgggcagg cgcagagtga
      301 gcagcgcgct gtcggcggcg cgggaagccg attccaaggc cgtgtgcgac tgcgccgccg
      361 gtcccggcgg cgcgaggaag tagcgcaggc ggacactgtc ggcgcgcgcc gcggccggaa
      421 catcatagaa gcccgagtag atgccggcga tccggagcga ctcggggacc gcaccctcgc
      481 cgaaaaccgc ttccccccag tagggctgga agcgcgggtc ggtctcggcc atcggcaccg
      541 agtcggcaac gccgggaatg gcgaaccagg cgcggcatcc aggggtggcc tggatccagg
      601 cctccacgcg atcgcccgcc gccaggtaca gatcgcccgc cggcgggcga tactcccggc
      661 cgaggcgaag gacatcgcgg gggtagctca cggccggcgc cggaatggcg acggtgatgg
      721 agcaggcgat cggggccgac ggttcgtttt cggggaacga cgtcttctgg cggtgctggc
      781 gcccggcgtc atcgtaaccg accgggcggg cctcgagccg gaaagtgaac gggccgggag
      841 cgatcggcag gaaagcgatg aaaccgccgc tgtcatgcac gggcacggcg gcgccgttga
      901 tggtgagccg ccactggtcg cggtgcgtgc acggcttgat gttgccgagg atgaaagtgg
      961 agtcgacggc ggcgaccgtc tggccctcct tgggatagat cacttcgatg cgcggcgcgg
     1021 ggccctgcgg ggccgcgggg gcgatgccga tccacgccag cacggccgcc gacgcgagca
     1081 gagtcctatt ccgagaggta tttctgcaat acatcgcgac gcaatttggg accgccgatg
     1141 atttcgcggt cgacgttgat ggactcgcgg tggtaactga tcccccgggc gatgtcgtcc
     1201 tcgagcagca gggggccgtc aagatcgaag tagtcggcca gggccgacat gtagaccgac
     1261 tgggcgatcc cgacggagga ctcgaccatg cagccgagca tgactttctt gcggtccgcc
     1321 tgggcctgcc gggcgagggc gacaccgtgg aggatgccgc cgcacttgtc catcttgata
     1381 ttgatgccgt cgatagaggc gcgacagcgc atgtaatcat cgagggtatt gagaccctca
     1441 tccatgatca gctcgacgtc ggccttaccc ttgaggtggg gccactcctt cacgtagtcg
     1501 gcatcggtcg gctgctcgat cacgcggacc cctttgcggc tgaggtggta aatcatctcc
     1561 tcggccttgg cgcacgacca ggcgccgttg gcgtcgacgc ggacttcttt gccgaggccg
     1621 gcctcaaggg cgtcgagtat catcacatcc tgttcgtgcc ccatcttgac cttgatgatc
     1681 gggtactcgg aggagcggat cgcttcgacc atccggtcgg tggcgtcgat gccgacggtc
     1741 atggagctct tgatgccgat aggtgtgccc aggtcgacca gctcccacgg gtagcggcgg
     1801 gattcgccgg agagatagtt caggacggcg gccatgaggg ccgaccgggc agcggggtgg
     1861 atatccatgc ggtcgatgtc atggagggtg ccgagggtga cggccgtctg gcgctcaagc
     1921 agtgcgatcc cctttctcag atcggcggcc atttcctcaa ttgagggtcc gtagtagatc
     1981 gaggtcgagg cctcgccgct gtagcggttg ttgaggatgg tgagcaggtt ggttttgacg
     2041 gtgacttcgc cctgggaaac cgcgaatttc ttcttgagcg ggaggttgac cttgaccgcg
     2101 tcgagctgca tcatataatt cgcctcgctt ccaaatagct ctggtccaaa tccgaccggt
     2161 acgcgggggc gtcgggcgcc agcgattcga tgcgcacgcc gccgccgccc cgggacgaat
     2221 gaataatgcg gtcgcgtccg acagcgatcg cgacgtgacc gggaaagaac accaggtccc
     2281 cgcagatggt acgcgcgcgc tcgaccggcc ggccggtgcg gatctggtct ttgctgtcgc
     2341 gcggcaggct caggccgagg cgatggcca
//
LOCUS       DAOOKS010000290         2367 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_55811_length_2367_cov_4.575260, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000290 DAOOKS010000000
VERSION     DAOOKS010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55811_length_2367_cov_4.575260"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1232
                     /locus_tag="PK112_05440"
     CDS             <1..1232
                     /locus_tag="PK112_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11526.1"
                     /translation="LNTYNLQGMEVGLLAFGVTLAMWLAARALQGGGALWPLFALLGG
                     LTLVRIDAAIPAAVLTAGVFWALPARRGEVVRWSALCVGGLLAAQTALRYWYYGDLLP
                     NTYYLKMTGYPARLRIAHGWEVLVGFLNRQYWLPFAAPLLAVRWLRHRAYLLLLAVIL
                     AQAAYSVYVGGDVWDWWGGANRFISIAMPLLFVALSLALATVAARLRPERKGNGGSLS
                     RPARRLFVAAAAVCLVLLNMLRGPASLSEWALTSRPLHWTDNANMVRRANLVRELTSE
                     NATIAVTWAGAIPYFSGRPTVDLLGKTDRRLAHGSAAERPPFRRWGEFLPGHMKWDYA
                     YSIGVLQPDVIVQLWGSATAAAPWLRDQYDVVPLGPFVFSFRREAPTVHWQRLAELSG
                     RTFSDGRGSIPPSPSDL"
     gene            complement(1297..2019)
                     /locus_tag="PK112_05445"
     CDS             complement(1297..2019)
                     /locus_tag="PK112_05445"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HPC11527.1"
                     /translation="MIRINKYLSQCGVTSRRGAEALIKEGRVAVNGRPLTEVGAVIEE
                     ATDEVRVDGEVVAPVEQQVYIVLNKPAEVMTTLRDPFRRRTVARFLKGVPCRVYPVGR
                     LDFDVEGVLLLTNDGDLAYRLTHPKYDVPKVYEAKVEGKFQRVDSEGIARGVALEDGA
                     IGRAAVTVLEYARRTTRVRLTLTEGRKREVKQLCAAVGHPVVYLERVEFAGITAKGLA
                     RGRWRHLTAAEIERLKSLVGLK"
     gene            complement(2051..>2367)
                     /locus_tag="PK112_05450"
     CDS             complement(2051..>2367)
                     /locus_tag="PK112_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11528.1"
                     /translation="MKEIEELIRAAEPDNGDQLSFETSVLDVAMARKLNVADGTFDPQ
                     TREADPQDDDRPAPPPGAPDNLGAEPPGADDADAEDELGDTALEEAAEQDESDSDAGR
                     HP"
BASE COUNT          295 a          844 c          807 g          421 t
ORIGIN      
        1 ccctcaacac ctacaacctg cagggaatgg aggtgggcct gctggcgttc ggggtcacgc
       61 tcgcgatgtg gctggcggcg cgggccctgc aggggggcgg cgctctctgg ccgctcttcg
      121 cgctgctcgg cgggttgacg ctcgtgcgca ttgacgcagc cattcccgcg gccgtcctca
      181 ccgccggcgt gttttgggcg ctcccggcgc gccggggaga ggtggtgcgg tggagcgcgc
      241 tctgcgtcgg cggcctgctc gccgcgcaga cggcgctgcg ctactggtac tacggcgacc
      301 tcctcccgaa tacgtactat ctgaaaatga ccggctaccc ggcccggttg cggatcgcgc
      361 acgggtggga ggtgctggtc gggttcctca accggcaata ctggctgccg tttgcggcgc
      421 ccctgctggc cgtgcggtgg ctgcgccacc gcgcctatct tctgctgctc gccgttatcc
      481 tcgcccaggc ggcctacagc gtctatgtcg gcggcgatgt ctgggactgg tggggcggcg
      541 cgaaccggtt catcagcata gccatgccgc tcctcttcgt cgccctgagc ctggccctcg
      601 cgacggtcgc ggcgcgcctg cggcccgagc ggaaaggcaa cggcgggtcg ctcagcaggc
      661 cggcgcggag actgtttgtc gcggccgccg ccgtctgtct ggtgctgctc aacatgctcc
      721 gcggtcccgc ctccctgtcc gagtgggcgc tgaccagccg ccccctgcac tggacggaca
      781 acgccaacat ggtgcgccgg gcgaatctgg tcagagagct gacgagcgaa aacgccacca
      841 tcgccgtgac ctgggccgga gcgatcccgt acttctccgg ccgcccgacc gttgacctcc
      901 tcgggaagac cgaccgccgg ctggcgcacg gctcggccgc cgaacgtccc ccgtttcgcc
      961 gctggggcga gtttctcccc ggccacatga agtgggacta tgcctactcg atcggcgttc
     1021 tgcagcccga tgtcatcgtg caattgtggg gttcagcaac cgcggccgcg ccctggctgc
     1081 gcgatcagta cgacgtcgtg ccgctcggac cgttcgtctt ctccttccgc agagaggccc
     1141 cgacggtgca ctggcagcgg ctggccgaac tcagcggccg gacgttctcc gatggccgcg
     1201 gcagcatccc cccgtccccc tccgacctgt agaaccggcc gcacgggcgg tctgccgccc
     1261 ggcgggcggg acggaccatc gggagtatcg ggggggctat ttcaggccga ccaggctttt
     1321 gaggcgctcg atctccgcgg ccgtgaggtg ccgccatctg ccgcgcgcca accccttggc
     1381 cgtgatcccg gcaaactcca cgcgctcgag gtagacgacc gggtgcccga ccgcggcgca
     1441 taactgcttg acctcccgtt tgcgtccctc ggtgagcgtc aggcggacgc gggtggtgcg
     1501 gcgcgcatat tccagcaccg tgaccgcggc ccggccgatg gccccgtcct cgagcgcgac
     1561 cccacgcgcg atcccctcgc tgtcgacgcg ctggaatttc ccctccactt tcgcctcgta
     1621 cactttcggc acgtcgtatt tcgggtgggt gagccggtag gcgagatccc cgtcgttggt
     1681 gagcaggagc acgccctcga cgtcgaaatc gagccgcccc accggataga cccggcaggg
     1741 aacacccttg aggaagcggg cgactgtgcg gcggcggaac gggtcgcgca gcgtggtcat
     1801 cacctcggcg ggtttgttca gaacgatgta gacctgctgc tcgacagggg cgacgacctc
     1861 gccgtcgacc cgcacctcat cggtcgcctc ctcgatgacg gctccgacct cggtgagcgg
     1921 ccggccgttg acggcgacgc ggccctcctt gatgagcgcc tcggcgcccc gccgcgaggt
     1981 gaccccgcat tgcgagaggt atttgttgat cctgatcact gatgcagccc tccggcggcc
     2041 gcccgggttg tcagggatgc cggcccgcgt cgctgtcgga ctcatcctgc tcggcggcct
     2101 cttcgagcgc ggtgtcgccg agctcgtctt cggcgtcggc atcgtccgcc ccgggaggct
     2161 ccgcgccgag gttgtccggc gcgccgggcg ggggcgccgg ccggtcatcg tcctgcgggt
     2221 cggcctcccg cgtctgcggg tcgaaggtgc cgtcggccac gttgagtttc cgcgccattg
     2281 ccacgtcgag caccgaggtc tcgaaactga gctggtcgcc gttgtccggt tcggcggccc
     2341 ggatcaattc ctcgatttct ttcatct
//
LOCUS       DAOOKS010000291         2364 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_55921_length_2364_cov_3.600260, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000291 DAOOKS010000000
VERSION     DAOOKS010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55921_length_2364_cov_3.600260"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="PK112_05455"
     CDS             complement(<1..325)
                     /locus_tag="PK112_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018701576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HPC11529.1"
                     /translation="MIQIFGTRKCPDTRKAERFFRERGVAIQVIDLREKGVSPGELRS
                     LARAVPLSELIDTEGKEYERLNLRYIRHNVEEMLLAHPLLFRTPIVRSGPRAAAGYHP
                     EIRQTF"
     gene            491..>2364
                     /locus_tag="PK112_05460"
     CDS             491..>2364
                     /locus_tag="PK112_05460"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HPC11530.1"
                     /translation="MSRVLAAAFCICLAASAAAEDLLEVTIGSHRDARALRESALTPV
                     ALTGNVYLALGERAEWKKLVEAGVRIEPVAFDVARPLLAFDRRPPGDARPGYPAVYDR
                     HGFRLVALPNRSALAGPHAADLAPLVRGPVAVRYVPPRPAPQPAAGAMGTIDSLVALI
                     SEDSLRSYTERLQAFHRRLTGTDSSAAARDWIAARFLAFGYDSVAIDPFVGEQLWDRV
                     PVPSYNVVACKVGALYPEQEIIIGGHFDAVPDCPGADDNATGTAGTMEIARVLAAGDF
                     PMTLKFIAFDSEESWMWGSYHYADSVAAEGAEIVLMINLDMIAHESNTAGANLYWGAD
                     QSYAALWADLAPTYGGIAASLMGGTASDHLPFAENGYDVIFVQERNFSTHYHEPSDST
                     THLNFEYMTRMVKVSLATAATVWTLPPPVRITAIREPGDGQSQLVQWRAIHSANIDHY
                     RVYWYPDGSPGNAHNLTLPAGDSSALVTGLTEGVTYVFGVRAYDVQGRTALAVEEVLG
                     RPDSRPAAPPGLAALPISHGVRVWWRHTDHELDFEHYAVYRDGTLAGTTADSVYEDRD
                     PALGTELHRYLVAAVDFDGNLSDTAAAVEARAATLTPGRILAINRDNVAVPGAEVALT
                     G"
BASE COUNT          377 a          809 c          790 g          388 t
ORIGIN      
        1 cgaaagtctg ccggatttcc ggatgatacc cggccgccgc gcgcgggcct gatcgcacaa
       61 tcggcgtgcg aaaaagaagg gggtgggcca ggagcatctc ctccacgttg tggcggatgt
      121 atctcaggtt cagccgctcg tactccttgc cctccgtgtc gatcagttcg gaaaggggca
      181 cggcgcgggc gaggctgcgc agctcccccg gactcactcc cttctcgcgc agatcgatga
      241 cctggatagc caccccgcgc tcgcggaaga accgttccgc cttgcgggtg tccgggcatt
      301 ttcgcgtgcc gaatatctga atcatcgacg gatccttttt ctcaacttag cgcggggcgg
      361 cccgggagag aacaaaaaag ccgagtctgc gccggccggt tcggggcgtt ttttttcaca
      421 accgtctgcg gcgcggccgt tgtgttttcg tatattggca gtagaaggaa cctcaatagg
      481 gaacagacct atgagtcggg tgctcgcagc ggcattctgt atctgcctgg cggcctcggc
      541 cgcggcggaa gatctcctcg aagtaacaat cggcagccat cgtgacgccc gggcgctgcg
      601 cgaatctgcg ctgaccccgg tggcgctgac gggcaacgtg tacctcgcgc tgggggagcg
      661 cgcggagtgg aagaagctgg tcgaggcggg ggtgcggatc gaaccggtcg cctttgatgt
      721 ggcgcggcct ctgctggcgt tcgaccgccg tccgccggga gatgcccggc ccggctaccc
      781 ggccgtgtat gaccgccacg gctttcgcct cgtcgctctg ccgaaccgct cggccctcgc
      841 gggcccgcac gccgccgacc tggcgccgct cgtccggggc ccggttgcgg tccgctacgt
      901 ccctccgcgc ccggcgccgc agccggccgc cggcgccatg ggcaccatcg acagcctggt
      961 cgcgctgatc agcgaagatt cgcttcggtc gtacaccgag cgcctgcagg cctttcaccg
     1021 ccgcctcacc ggcaccgatt cctccgccgc ggcgcgcgac tggattgccg cccggttcct
     1081 ggccttcggc tacgactcgg tggcgatcga tccctttgtc ggcgagcagc tctgggaccg
     1141 ggtcccggtg ccgagctaca acgtcgtggc gtgcaaagtc ggggcgctct acccggagca
     1201 ggagatcatc atcggcggcc atttcgacgc ggtgccggat tgtcccggcg ccgacgacaa
     1261 cgccaccgga acggcgggca cgatggagat cgcgcgcgtg ctcgccgccg gggacttccc
     1321 catgaccctc aaattcatcg cgttcgactc ggaagagtcc tggatgtggg gatcgtacca
     1381 ctacgccgat tcggtggcgg ccgagggggc ggaaatcgtc ctcatgatca acctcgacat
     1441 gatcgcgcat gaatccaaca ccgccggagc gaacctctac tggggagccg accagtccta
     1501 cgcggcgctc tgggcggatt tggcgccgac ctacggcgga attgccgcga gcctgatggg
     1561 gggcacggcc tccgaccacc tgccgttcgc ggagaacggc tacgacgtga ttttcgtcca
     1621 ggagcgcaac ttctcgaccc actaccacga gcccagcgac agcaccaccc acctcaattt
     1681 cgaatacatg acgcgcatgg tgaaagtgag cctggcgacc gcggcgacag tctggacgct
     1741 gcctccgccg gtgcgcatca cggccatccg ggagccgggc gacggccagt cgcaactggt
     1801 ccagtggcgc gcgattcaca gcgccaacat cgaccactac cgggtgtact ggtacccgga
     1861 cggttctccg ggaaacgcgc acaacctcac cctccccgcc ggcgacagca gcgcgctggt
     1921 gaccgggctc accgaaggag tcacgtatgt cttcggcgtg cgggcgtacg acgtgcaggg
     1981 gcggacggcg ctggcggtgg aggaggtgct cggacggccc gattcgcggc cggccgcgcc
     2041 gcccggactg gcggcgctcc cgatctctca cggggtccgg gtctggtggc gccacaccga
     2101 tcacgaactc gatttcgagc actacgccgt ctaccgcgac ggcacgcttg caggcacgac
     2161 cgccgacagc gtgtacgagg accgcgaccc cgcgctggga accgagctgc accgctacct
     2221 ggtcgcggcg gtcgacttcg acggcaacct gtcggacacc gcggcggctg tcgaggcccg
     2281 ggcggcgacc ttgacgccgg ggcggatact ggcgatcaac cgcgacaatg tcgcggttcc
     2341 cggagccgag gtcgcgctca ccgg
//
LOCUS       DAOOKS010000292         2358 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_56100_length_2358_cov_3.032566, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000292 DAOOKS010000000
VERSION     DAOOKS010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56100_length_2358_cov_3.032566"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="PK112_05465"
     CDS             complement(<1..710)
                     /locus_tag="PK112_05465"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HPC11531.1"
                     /translation="MKRLLLVLSLLGMMTAFFGCSNDPQTTGPAVGQDTPGTLVQRPE
                     FIDNRPDAVKAAFTKLGTPIESSDRVVADGFSAADPNPTPAHKYAYVIGISDYDGTAN
                     DLNFCDDDARDVIAFLNSQGFTVRSDIDYSASAANIQAGLIWLRDAAVPGDEVAFFYS
                     GHGTKVRPSGGSSIISRDLYYLTHAYVMGYLNAANCTKKTINLDACVIGDFHADCKTG
                     TVLSTASVSKYSYDAPDL"
     gene            complement(1488..>2358)
                     /locus_tag="PK112_05470"
     CDS             complement(1488..>2358)
                     /locus_tag="PK112_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11532.1"
                     /translation="YQNACVNAYRIGICEEPGAVRYAARVETVNRDRRMLTFAGKPDS
                     VIANLNCAVVRLNGNSEAPVPFDEINPGDSVQLYGERNQNRYVYARHLGVCSPGGGGY
                     DVAVRDTITSIDYAAGTFTIAGRTELITVDSETVIWTNLRILHEQPDENGRDRMGMAL
                     SDGGNFYRSDHDSILAFTDVAVGDVIELRADIVNETTLRAAVIKLTPECLSRCLAFSD
                     RLATIDVAARLVTFENETRICWVCPGAKLRDRDGAEITLADFAVGDFVAVKGYPLAGD
                     TLKISQMALTAEP"
BASE COUNT          435 a          711 c          779 g          433 t
ORIGIN      
        1 tgcagatcgg gggcgtcgta ggagtatttg gatacggagg cggtggagag cacggtgccg
       61 gttttgcagt cggcatggaa gtcgccgatg acacaggcat cgagattgat cgtcttcttc
      121 gtgcagttgg ccgcgttgag gtagcccatg acgtatgcgt gggtcaggta gtacaggtcc
      181 cgcgagataa tgctcgaacc acccgacggg cgcactttgg tgccgtggcc ggaatagaag
      241 aaggccacct cgtcgccggg aaccgccgcg tcgcgcaacc agatcaggcc cgcctggatg
      301 ttggccgccg aggcgctgta gtcaatgtcg ctgcgcacgg tgaaaccctg gctgttcagg
      361 aacgctatga catcgcgcgc atcgtcatcg cagaaattca gatcattggc cgtgccgtcg
      421 taatcggaga tcccgatgac atacgcatac ttgtgagccg gggtcgggtt gggatcggcg
      481 gcggaaaagc cgtcggcgac cacccggtca gacgactcaa tgggtgtgcc cagcttggtg
      541 aatgcagctt tcaccgcatc cgggcggtta tcgatgaact cggggcgctg aaccagggta
      601 ccgggggtgt cctggccgac agcgggaccg gtcgtctgcg ggtcgttcga gcaaccaaag
      661 aacgccgtca tcatgcccaa cagactcaga accagtaata gccttttcat gtgagacctc
      721 tccttagggt tgatttgaaa acactgatga ggagggagtt caccccagag ccaaacgcga
      781 cgacctcctc tcccgaagga cacgacggct tggttgcttc gacagcccgt ccccccgtat
      841 cgtagccgga ggacggacgc cgcagctgtg agtgctgccc caatatatgc tctgtccaac
      901 aaatctgtcc acgctttttt cgtcggcccg gccgaaattc gcctgcccgg ttcggcgtca
      961 ccaggcgcaa gagcacaacg gggcaaggag gtttccggcg accccggccg caggcccggc
     1021 gagttcttgt gaaagtcaaa ttccaggcgg gcatccggcg ttcggttccc cttggctccg
     1081 accctgcgcc cggccgcgag gggagcgtga tggatacgtc atgctgcgtg atggagggct
     1141 ccggattagg ctgtgcgccg ggcaatccgg tcgtggggaa gcagtggccg tgagcgagtc
     1201 cctcccccga accgagaaga tacctgcgca ccagccgggc gacttcgtgc gtgagcttgc
     1261 tcccccctca gccacgggat agccgggtcc ggagggcggg cgggggaacg cgccaaaaag
     1321 acaaagagga aggccggacc ctgatccggc cctccatcct gagaccgtcc agctcaagac
     1381 cggtctactc tctccatgcc tgcccggata gcgacgaaag catcggcgtc cggcggggag
     1441 ggagtccgga cgccgagggc gtctcaccga ctttgtggtg gagacgtcta cggttctgcc
     1501 gtcagcgcca tctgggagat cttcaacgta tcgcccgcca ggggatagcc cttgacggca
     1561 acgaaatcgc cgaccgcgaa atcggcgagg gtgatctccg ctccgtcacg atcccgcaat
     1621 ttcgcccccg ggcagaccca gcagatccgc gtctcgtttt cgaaggtgac gagccgagcg
     1681 gcgacatcga tcgtcgcgag gcggtcgctg aaggcgaggc agcggctcag acactcgggc
     1741 gtcagtttga tcaccgccgc acggagggtg gtttcgttga cgatgtcggc ccgcaattct
     1801 atcacgtcgc cgaccgcgac gtcggtgaag gccagaattg agtcgtggtc ggagcgatag
     1861 aaattgccgc cgtccgacag cgccataccc atgcggtcgc gcccgttttc atccggctgt
     1921 tcgtggagga tccggaggtt ggtccatatg acggtctcgg agtccacggt gatgagttcg
     1981 gtgcggccgg cgatcgtgaa ggtgccggcg gcgtagtcga ttgaggtgat cgtgtcccgc
     2041 acggcgacat catagccgcc tccgccgggg ctgcacacgc ccaggtggcg ggcgtagaca
     2101 tagcggttct ggttgcgctc gccatagagc tgcacggaat cgcccggatt gatctcgtcg
     2161 aacgggacgg gcgcctcgct gttaccgttg aggcggacta ccgcgcagtt caggttggcg
     2221 atgacagaat ccggtttccc ggcgaaagtc agcatgcggc ggtcccggtt gaccgtctcg
     2281 acgcgcgccg cataccgcac cgctcccggt tcctcacaga tcccgatccg gtaggcgttc
     2341 acgcaggcgt tctggtag
//
LOCUS       DAOOKS010000293         2357 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_56136_length_2357_cov_2.840139, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000293 DAOOKS010000000
VERSION     DAOOKS010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56136_length_2357_cov_2.840139"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="PK112_05475"
     CDS             complement(<1..193)
                     /locus_tag="PK112_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylceramidase"
                     /protein_id="HPC11533.1"
                     /translation="MTDGTLTPVDGAATVTVNEGSPAQIWEGFGGAFNEMGWHYLLML
                     SQGVRDEALQLLFGAEGCRF"
     gene            complement(841..1809)
                     /locus_tag="PK112_05480"
     CDS             complement(841..1809)
                     /locus_tag="PK112_05480"
                     /inference="COORDINATES: protein motif:HMM:TIGR03901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MYXO-CTERM sorting domain-containing protein"
                     /protein_id="HPC11534.1"
                     /translation="MPRPLPLFIRAAAGAVMLAAALAGCGEKDVPVVVDRDILLRYLA
                     TSEDAVDLFRTTGLFPTEEYRIPNDPARYRIVVDSTRRIITLDTEIDTVKKRTWPDGS
                     VEYYRSYHDYGNPFGRLWASEATVDDYFYVRLSRTEDEETATASHVWAITRYGYFLKL
                     GGDDQPYVGWKLWAYNGGAAPTGGVATTGGTTPTGGVPTTGGTMTTGGIAPTGGVAVT
                     GGGPATGGALPATGGSATGGNAAGGVTPTAGGTATTGGAVATGGASAVGGATGGDAAP
                     AASANGDDDPGCGCRVPARRSSPFGQAGWLAVAVLGLARLRRRPGR"
     gene            complement(1813..>2357)
                     /locus_tag="PK112_05485"
     CDS             complement(1813..>2357)
                     /locus_tag="PK112_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HPC11535.1"
                     /translation="RLAEFDVLVGVNLLREGLDLPEVSLVAILDADKEGFLRSERSLI
                     QTAGRAARNKGGEVILYADKVTESMRKAIEETRRRREKQAAYNREHGIDPETIFKTRE
                     EILRSTQFADSKTPEEAPRFEKPSSFTRMSREDQLAFMLAAMRKAAENLDFETAIMIR
                     DEINALRGDGRKNPKPKKGK"
BASE COUNT          297 a          887 c          794 g          379 t
ORIGIN      
        1 cgaaccggca cccctccgcc ccgaacagga gctgcagggc ttcgtcccga acgccctggc
       61 tgagcatcag gaggtagtgc cagcccatct cgttgaaggc gccgccgaag ccctcccaga
      121 tctgagctgg cgacccttcg ttgacggtca ccgtcgcggc gccgtcaacc ggggtgagag
      181 tcccgtcggt cacccagggc gccccgctgg tcgaggtgac cagcagcggc tcggtcggaa
      241 cgccccctcc ggttccttcg ccgccagtct gcgcgccgcc ggtttccgcg ccgcccgaat
      301 cacttccgcc tgacgaaacc ccgcccgtat cgctcccgcc cgaggcggcg ccgcccgtct
      361 cgcccccgcc cgacggggcc ccaccggtgc cctgtccgcc cgtgatgccg cccgacgcgg
      421 cggagccacc cgtcgcacca ccgccgccgc tgctggtgcc gccggcgctg gtgccgccgg
      481 tccctgtccc acccgtgccg cccacgacac cccccaccgg accaccgccg atgggcgtgg
      541 cgcccccggt ggcgccaccc gttgcggtgc cgccgtaagc ctctccgcct gttcccaagg
      601 cgcccccggt cgggctcgtg ccccctgacg gggtcgcgcc ccccgtggcg ccggagctcc
      661 ctcccgtggc gccggatgag cctccaaggc ctcccgtggg ctcctcgccg tcggacccgg
      721 agcccgagca gccggcgagg aagagcatcg ccgtacaggc ggtcagcaag gttcgcatca
      781 attcctccgc gagaatggga cgagtcgtcg attggcgcct gggctgcgcg gcctcgtcgt
      841 tcaacgaccc gggcggcgcc gcaggcgcgc cagcccgagc accgcgactg ccagccagcc
      901 cgcctggccg aacggcgagc ttcgtcgcgc ggggacgcgg cagccgcagc cggggtcgtc
      961 atcgccattc gcgctcgccg cgggcgcagc gtcgccgccg gtcgcaccgc ccacggcgct
     1021 ggctccgccc gtcgccaccg ccccgcccgt cgtggcggtg ccgccagcgg tcggtgtgac
     1081 gccacccgcg gcgttgccgc ccgtggcgct accgcccgtc gccgggaggg cgccccccgt
     1141 cgcggggccg ccgcccgtca ccgcgacacc gcccgtcggt gcgatgccgc ctgtcgtcat
     1201 cgtcccaccc gtcgtgggga cgccaccggt cggcgtcgtc ccgcccgtcg tcgctacgcc
     1261 acccgtcggc gccgcgcccc cgttgtaggc ccacagtttc catccgacat acggctggtc
     1321 atcgccgccc agtttcagga agtagccgta gcgcgtgatc gcccaaacgt gcgacgccgt
     1381 ggccgtctcc tcgtcctcgg tgcgcgacag ccggacgtag aagtagtcgt cgacggtggc
     1441 ctccgaggcc cagaggcgcc cgaagggatt gccgtagtca tggtagctgc ggtagtactc
     1501 caccgagccg tccggccagg tgcgtttctt cacggtgtcg atttcggtgt caagcgtgat
     1561 gatccgccgg gttgaatcga cgacgatgcg gtaccgggcg ggatcgttcg gaatccggta
     1621 ttcctcggtg gggaacagac cggtggtgcg gaagaggtcg acggcatcct ccgaggtggc
     1681 gaggtaccgg agcagtatgt cgcggtcgac gacgacggga acgtccttct ctccacagcc
     1741 cgcgagcgcg gcggccagca tgacggcgcc ggcggcggcg cggatgaaca gaggcagtgg
     1801 tcgcggcatc ggctattttc cctttttcgg tttcgggttt ttccgcccgt ctccgcgcag
     1861 cgcattgatt tcgtcgcgga tcatgatggc cgtctcgaag tcgaggttct cagccgcctt
     1921 gcgcatggcc gcgagcatga acgcgagctg gtcctcgcgg gacatgcggg tgaaactgct
     1981 cggtttctcg aagcgcggcg cctcctcggg cgtcttgctg tcggcgaact gggtggagcg
     2041 gaggatctcc tcgcgcgtct tgaaaatcgt ttccgggtcg atgccgtgct cgcggttgta
     2101 ggcggcctgc ttctcgcggc ggcggcgggt ctcctcgatc gccttgcgca tcgattcggt
     2161 gactttgtcg gcgtacagga tcacctcccc gcccttgttg cgggcggccc gtccggccgt
     2221 ctggatgagc gaccgctccg agcggaggaa tccctccttg tcggcgtcga gaatggccac
     2281 cagtgacacc tcggggaggt cgagtccctc ccgcaagagg ttcactccga caaggacgtc
     2341 gaactccgcc aggcgga
//
LOCUS       DAOOKS010000294         2352 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_56290_length_2352_cov_3.011319, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000294 DAOOKS010000000
VERSION     DAOOKS010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56290_length_2352_cov_3.011319"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..251
                     /locus_tag="PK112_05490"
     CDS             3..251
                     /locus_tag="PK112_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007294289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HPC11536.1"
                     /translation="MFDEDGLTMGRIDYAGTVRTACLDSVPEVRVGQYVLVHAGFALN
                     VLDEDEARRTLDLFDEIARQNAEEGLDPFGEPLPPEAP"
     gene            257..1352
                     /gene="hypD"
                     /locus_tag="PK112_05495"
                     /pseudo
     CDS             257..1352
                     /gene="hypD"
                     /locus_tag="PK112_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563173.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
     gene            1366..>2352
                     /gene="hypE"
                     /locus_tag="PK112_05500"
     CDS             1366..>2352
                     /gene="hypE"
                     /locus_tag="PK112_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HPC11537.1"
                     /translation="MPDEPLPEPSFSCPLPLSAPATIQLAHGSGGRLANDLIARLFVE
                     AFDNPMLRRQDDQATVEIDGLRLSFSTDSFVVDPLFFPGGDIGDLAVNGTVNDLSMNG
                     ARPLFLSAGFIIEEGLPIADLARVVQSMRKAAAAAGVQIVTGDTKVVNRGKGDKLFIN
                     TAGIGVISHPYDIGVDRLRPGDRVLISGPLGNHGLAVLSRREGLAFESPIVSDTAPLN
                     GLVAALVAGVGGALHAMRDPTRGGLAATLNEFARASRVGIRLVGAQVPIDPPVAGACE
                     LLGLDPLYVANEGKLTAVVSAEAADRALALMRAHPLGARAAVIGEVHADRPGE"
BASE COUNT          316 a          880 c          788 g          368 t
ORIGIN      
        1 agattttcga tgaagacggc ctgacgatgg gccggatcga ctacgccggg acggtgcgca
       61 ccgcctgtct cgattcggtg cccgaggtgc gggtggggca gtacgtgctc gtccatgccg
      121 gcttcgccct caacgtgctc gacgaagacg aggcgcgccg gacgctcgat ctcttcgatg
      181 agatcgcccg gcagaacgcc gaggaggggc tcgatccgtt cggggagccc ctgccgccgg
      241 aggcgccgtg accggcctga agtatctcga tgaatttcgg gatccccggc gggccgcggc
      301 cctgctcggc gagatccggc gcaccgtcac ccgaccctgg gtgatcatgg agatctgcgg
      361 cggccagacg cactcgatca tccgcaacgg cctcgaccag cttttgccgc cgacgatccg
      421 gctcgtccac ggtccggggt gcccggtgtg cgtgacctcg ctcgagctca tcgaccgcgc
      481 tctggccatc gccgcccgcc ccgatgtgat cttcgcctcc ttcggtgaca tgttgcgcgt
      541 gccgggatcg tcgaaagacc tcgaccgtga aatccgaggg tggcgatgtt cgcatggtgt
      601 attcgccgct ggacgccctc cgtctcgccc gcgcgcaccg cgaccgacgg gtggtgtttt
      661 tcgccgtggg tttcgagacc accgccccgg cgaccgccac cgccatccgc caggcgcgcc
      721 gcgaggggct ggacaacttc ctgttgctgg tcgcccacgt cctcgtgccg ccggcgatgc
      781 gggcgctcct cgaggcgccc ggcaaccgcg tgcaggcgta cctggccgcc ggccacgtct
      841 gcgccgtcac cggctgggag gagtactgcc cgatcgcgga gggctaccgc gtgccggtcg
      901 tggtcaccgg cttcgagccg gcggacattc tcgacggcat cctggccgcc gtgcgccagc
      961 tcgagaccgg gcaggcccgg gtcgagaacc gctacgcgcg ggctgtccgg gagcagggca
     1021 acccgcacgc gcgcgccgcc gtcgaggctg tgtacgaagt gtgcgaccgg aagtggcgcg
     1081 ggatcggaac catcccgcag agcggctacc gcctccgccc cgaattcgcc gcccatgacg
     1141 ccgagcgcat attccggctg gccgatctcg aggtgctcga gccccccgaa tgcatcagcg
     1201 gcctcgttct ccagggcctg cggcggccgg tcgattgccc ggcgttcgcc cggcgctgca
     1261 caccgcagac gccgctcggc gccaccatgg tgtcctccga gggcgcctgc gccgcctact
     1321 accgctacca ccgccgcgcg gcggaaagct gaagggttac ccgctatgcc cgatgaacct
     1381 ctccccgagc cgtccttctc ctgcccgctg cccctctcgg cccccgcgac catccagttg
     1441 gcccacggca gcggcggccg gctcgccaac gacctgatcg cgcggctctt tgtcgaggct
     1501 ttcgacaatc cgatgctgcg gcggcaggat gaccaggcca cggtggaaat cgacggcctg
     1561 cgcctctcct tctcgaccga ttccttcgtc gttgacccgc tcttctttcc cggcggcgac
     1621 atcggcgacc tggccgtgaa cggcaccgtc aacgacctct ccatgaacgg cgcgcgcccg
     1681 ctctttttga gcgccgggtt catcatcgag gaggggctgc cgatcgcgga cctggcccgc
     1741 gtcgtgcagt ccatgcggaa ggcggcggcc gccgccggcg tgcagatcgt caccggcgac
     1801 accaaggtcg tcaaccgggg caaaggggac aagctgttca tcaacacggc cgggatcgga
     1861 gtcatttccc acccctacga catcggcgtc gaccggctcc ggccggggga tcgcgtgttg
     1921 atcagcgggc cgctcggcaa ccacggcctg gcggtcctct cccggcgcga gggtctcgct
     1981 ttcgaaagcc ccattgtcag cgacacggcc cccctcaacg ggctcgtggc cgcgctggtc
     2041 gccggggttg gcggcgcgct ccacgccatg cgcgatccga cccgcggcgg gctggccgcc
     2101 accctcaacg aattcgcccg cgcctcccgg gtcggcatcc gcctggtcgg ggcgcaggtg
     2161 ccgatcgacc cgcccgtcgc cggcgcctgc gagctgctcg gcctcgaccc cctctacgtc
     2221 gccaacgagg gcaagctgac ggcggtggtg agcgccgagg cggccgatcg cgccctcgcc
     2281 ctcatgcgcg cccatccgct cggcgccagg gcggcggtca tcggcgaggt ccacgccgac
     2341 cggcccggag ag
//
LOCUS       DAOOKS010000295         2342 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_56650_length_2342_cov_2.822038, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000295 DAOOKS010000000
VERSION     DAOOKS010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56650_length_2342_cov_2.822038"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="PK112_05505"
     CDS             <1..609
                     /locus_tag="PK112_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HPC11538.1"
                     /translation="GKALERRLPPIVVINKIDSAEPEGVAEVRANVSAYNPDAVVIDA
                     ASPLDVDKPALIKGKRVLVVEDGPTLTHGEMAYGAGVVAAEKFGAAELVDPRPYTVNS
                     ISETFKKYPDIGVLLPAMGYGPKQIKDLETTINRTECDAVVIATPIDLSRIIKIRKPT
                     VRVGYSLQEIGRPNLADVLGEFVGRQKKGAAKAPAPRKKARG"
     gene            702..1631
                     /gene="arcC"
                     /locus_tag="PK112_05510"
     CDS             702..1631
                     /gene="arcC"
                     /locus_tag="PK112_05510"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HPC11539.1"
                     /translation="MVALGGNAITRPDQEDTIANQFANTRKSLDGIVDLARDGFNLVI
                     SHGNGPQVGNALLRVELARGRAPILPLGVLVADTEGGMGYMIEQSLQNRLRSEKIDRR
                     VVTLITQMLIDMNDPALQNPTKFIGQFYTEEEARRFAKERGWTMKQDAQRGWRRVVPS
                     PKPTAVVNGETIRELVARGIIVIAAGGGGIPVYLDGRGYYEGFDAVIDKDLASAVLAN
                     EIGADILCILTAVDHVAVAFGTPQEKKLEEVTVTRMRQYLREGHFPPGSMGPKVEAAV
                     KFIEHGGKLVIITSLENAHAAVHGQAGTRIRPD"
     gene            1633..>2342
                     /locus_tag="PK112_05515"
     CDS             1633..>2342
                     /locus_tag="PK112_05515"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11540.1"
                     /translation="MNELSREAIEAVLEKGRMYEVGGTVRDRLLAGSPASADRDFLVT
                     GIGYDELSRLLRQFGQVNLVGRSFGVIKYTEYRDGAPVTFDISLPRTEHSTGVGHKDF
                     EVRYDPALAIEDDLVRRDFTINAMAIALGSDEIIDPLGGQEDLRKRLVRMVYPESFTD
                     DPLRMLRAVGFAARFEFDIEERTFAAIAAKAPLIRTVSAERIAEELRKLLERAARPSR
                     GLQLMERSGLLAEVLPEL"
BASE COUNT          456 a          711 c          845 g          330 t
ORIGIN      
        1 ggcaaggcgc ttgagcggag gctgcctccc attgtcgtga tcaataagat cgactcggcc
       61 gagccggaag gggtggccga ggtgcgggcc aacgtcagtg cctacaatcc ggacgcggtc
      121 gtgatcgacg cggcctcgcc gctcgatgtc gacaagcccg cgctgatcaa gggcaagcgg
      181 gtgctcgtgg tcgaggacgg gccgacactc acccacggcg agatggccta cggggcgggc
      241 gtggtggcgg ccgagaaatt cggcgcggcc gaactggtcg atccccggcc ctacacggtc
      301 aactcgatct cggagacatt caagaagtac ccggacatcg gagtcctcct cccggccatg
      361 ggctacggcc cgaagcagat caaggatctg gagacgacga tcaaccggac ggagtgcgac
      421 gcggtggtga tcgccacgcc gatcgacctg tcgcggatca tcaagatcag gaaaccgacg
      481 gtgcgggtgg gttacagcct gcaggagatc gggcggccga acctggcgga cgtgctgggc
      541 gaatttgtcg gacggcagaa gaaaggggcg gccaaggcgc cggccccgag gaagaaagcc
      601 cgcggatgac cacggcgcgg gccggggaaa acccccggcc cgccggcatg aactgcaacg
      661 aggacagacc aatcaggcag acagcagcaa acagaacggc agtggtggcg ctcggcggga
      721 acgcgatcac ccggccggac caggaagaca cgatcgccaa ccagttcgcg aacacgcgga
      781 aatcgctgga cgggatcgtg gatctggcgc gcgacggttt caacctggtg atctcgcacg
      841 gcaacgggcc gcaggtcggc aacgcgctct tgcgggtgga actcgcccgc ggacgggcgc
      901 cgatcctgcc cttgggcgtg ctggtggcgg acaccgaggg cgggatgggc tacatgatcg
      961 agcagtcgct ccagaaccgg ctgcgcagcg agaaaatcga ccggcgcgtg gtcaccctca
     1021 tcacccagat gctcatcgat atgaacgacc cggccctcca gaacccgacc aaattcatcg
     1081 ggcagttcta caccgaagag gaggcgcggc gcttcgccaa ggagcgcggg tggaccatga
     1141 agcaggatgc tcagcggggg tggcggcgcg tggtcccgtc gccgaaaccg accgccgtgg
     1201 tcaacggcga gacgatcagg gagctggtcg cccgcggtat catcgtcatc gccgccggcg
     1261 gcggcggcat cccggtctac ctcgacggcc ggggatacta cgaggggttc gatgcggtga
     1321 tcgacaaaga cctcgcctcg gcggtgctgg ccaacgagat cggcgccgac atcctgtgca
     1381 tcctcaccgc cgtcgaccac gtcgccgtcg ctttcggcac cccgcaggag aaaaagctcg
     1441 aagaagtgac ggtgacgcgg atgcggcagt accttcgcga gggacacttc cccccggggt
     1501 cgatggggcc gaaggtggaa gcggcggtga aattcatcga gcacggggga aaactcgtga
     1561 tcattacgtc gctggaaaac gctcacgccg cggtgcacgg ccaggcggga acgcgcatac
     1621 gcccggattg aaatgaacga attgtcacgg gaagcgatcg aggcagttct ggagaaaggc
     1681 cgcatgtacg aggtcggagg aacggtccgg gaccggctgc tggccgggag tccggcgagc
     1741 gcggaccggg atttcctcgt cacggggatc gggtacgacg agttgtcgcg cctgctgcgg
     1801 cagttcggcc aggtaaacct ggtgggccgc agtttcgggg taatcaagta cacggagtac
     1861 cgggacgggg cgccggtgac gttcgacatt tcgctgcccc ggacggagca ctcgacgggg
     1921 gtggggcaca aggatttcga ggtgcggtac gatcccgcgc tggcgatcga agacgacctc
     1981 gtgcggcggg atttcacgat caacgcgatg gcgatcgcgc tggggtccga cgagatcatc
     2041 gacccgctcg gcgggcagga agacctgcgc aagcggctgg tccggatggt ctacccggag
     2101 tcattcaccg acgacccgct gcgcatgctg cgggcggtgg ggtttgcggc gcgcttcgaa
     2161 ttcgacatcg aggagcggac gtttgcggcg atcgcggcca aggccccgct tatccggacg
     2221 gtctcggccg agcggatcgc cgaggagctg cgcaaactgc tcgagcgggc ggcccgcccc
     2281 tcgcgcggac tccagctgat ggaacggagc gggctcctgg cggaggtcct ccccgaactg
     2341 ca
//
LOCUS       DAOOKS010000296         2336 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_56832_length_2336_cov_4.072775, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000296 DAOOKS010000000
VERSION     DAOOKS010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56832_length_2336_cov_4.072775"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="PK112_05520"
     CDS             <1..458
                     /locus_tag="PK112_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPC11541.1"
                     /translation="SGGERLTVGAVSAIVFAGRVGAGVAAELGTMRVTEQIDALESMG
                     IDPVRFLVMPRILACLTMVPFLVVFANFIAILGGMGVAVLGVDISAEMFLDGFHSSFS
                     LWISSTGRSRRPCSACSSGWSGATRGSTPKAGRPAWGRRPPPRSSSRRC"
     gene            509..1285
                     /locus_tag="PK112_05525"
     CDS             509..1285
                     /locus_tag="PK112_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPC11542.1"
                     /translation="MDTLRPQAESVCFEVEDLHKAFGAQRVLDGVSLKVQTGESVAII
                     GQSGSGKSVLLKHLIRLLVPDRGRVLYRGRDLADMSSGELVEVRRKIGMLFQSAALFD
                     SMTVAENVGLELREARRHEQREIDRIVAEKLEMVGLAEAGDKYPAELSGGMRKRVGLA
                     RAIANDPDVLLYDEPTTGLDPVTADVINDLIVNLNRQLRVTSVVVTHDMKSAFRIAGR
                     VVMLYDARVRFDGSPQELQRAGDPVLRQFIAGDSAGPIRI"
     gene            1303..>2336
                     /gene="radA"
                     /locus_tag="PK112_05530"
     CDS             1303..>2336
                     /gene="radA"
                     /locus_tag="PK112_05530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HPC11543.1"
                     /translation="MADKRSGKVKTAFFCTQCGAEHTRWQGQCRECAEWNTLVEERLV
                     KPAGGKGRGTRPPAEARRLPEISLAAEAGIESGIGEFDRVLGGRLLPGMSVLIGGEPG
                     IGKSTLLLQAAEAYSRRGHRVAYVTGEESLAQIRLRAERLGVAGENIVAVNANALEEI
                     CDLVAEGAYEIVLVDSIQTVASAMFDSPPGTVAQVRESAQRLMGLARQHGFALFLVGH
                     VTKDGMVAGPKVLEHMVDTVISFEGDGSHLYRMLRVMKNRFGSVAEIGLFEMQSRGLV
                     EVTNPSSLFLSQRQDARRTGSVVAGICEGQRPLLVEIQALVTAASYGNPQRVAGGIDN
                     KRLALLIAIL"
BASE COUNT          361 a          662 c          913 g          400 t
ORIGIN      
        1 tctcgggcgg ggagcggctc acggtggggg cggtctcggc gatcgtgttc gcggggcggg
       61 tcggcgccgg ggtggcggcc gagctgggga cgatgcgggt caccgagcag atcgacgcgc
      121 tcgagtcgat ggggatcgac ccggtgcgct ttctcgtcat gccgcgcatc ctcgcctgcc
      181 tgacgatggt gccgttcctg gtcgtgttcg ccaatttcat cgccattctc ggcggcatgg
      241 gggtggcggt gcttggggtg gacatttcgg cggaaatgtt tctcgacggc tttcacagct
      301 cgttcagcct ctggatttca tcaacgggga gatcaaggcg gccatgttcg gcctgctcat
      361 cgggctggtc gggtgctacg aggggttcaa caccaaaggc ggggcggccg gcgtggggga
      421 ggcgaccacc acctcggtcg tcatctcgtc ggtgctgatc ctggtctcga atttcctttt
      481 cgccatcgtg ctcttccggt tttgacctat ggatacacta cggccccagg cagagagcgt
      541 ctgtttcgag gtggaggatc tccacaaggc gttcggcgcg cagcgggtgc tcgacggcgt
      601 cagcctgaaa gtgcaaaccg gggagtcggt ggcgatcatc gggcagtcgg gctcggggaa
      661 atcggtgctg ctgaaacacc tgatccggct gctggtgccg gaccgcggcc gcgtcctcta
      721 ccgggggcgg gacctcgccg acatgagttc cggcgagctg gtcgaagtgc ggcggaaaat
      781 cggtatgctc tttcagtcgg cggcgctgtt cgattcgatg acggtggcgg agaatgtcgg
      841 gctggaactg cgggaggcgc ggcggcacga gcagagagag atcgaccgga ttgtcgcgga
      901 gaagctcgag atggtcgggc tggccgaggc gggggacaaa tatcccgccg agctgtcggg
      961 cggcatgcgc aagcgggtgg ggctggcccg ggcgatcgcc aacgacccgg acgtgctgct
     1021 gtacgacgaa ccgacgaccg ggctggaccc ggtgacggcc gacgtaatca atgacttgat
     1081 cgtcaacctc aaccggcagc tgcgggtgac ctcggtggtg gtgacgcacg acatgaagtc
     1141 ggcgttccgg atcgccggcc gggtggtgat gctctatgac gcgcgggtgc ggttcgacgg
     1201 cagcccgcag gaactgcagc gggcgggcga cccggtgctc cgacaattta tcgccggcga
     1261 ctccgccggg ccgatacgga tatgacggga agtcgggcgg cgatggcgga caagcgcagc
     1321 ggcaaagtga agacggcgtt tttttgcacc cagtgcgggg ccgagcacac ccgctggcag
     1381 gggcagtgcc gggagtgcgc ggagtggaac acgctggtcg aagagcggct ggtcaaaccg
     1441 gcggggggca aggggcgggg gacgcgcccg ccggccgagg cgcggcgact tccggaaata
     1501 tcgctcgccg cggaggcggg gatcgaaagc gggatcggcg aattcgaccg cgtgctgggc
     1561 ggacggctgc tgccggggat gagcgtgttg atcggcggcg agccggggat cgggaagtcg
     1621 acgcttctct tgcaggcggc cgaggcctac tcgcggcggg ggcaccgggt cgcttacgtc
     1681 acgggcgagg aatcgctcgc ccagatccgc ctgcgggccg agcggctcgg cgtggccggc
     1741 gaaaacatcg tggctgtcaa cgccaacgcg ctggaagaaa tctgcgacct ggtggccgag
     1801 ggcgcttacg agatcgtgct cgtcgactcg atccagacgg tggcctcggc gatgtttgac
     1861 tcgcccccgg ggacggtcgc gcaggtgcgg gagtcggccc agcggctgat gggactggcg
     1921 cggcagcacg ggttcgccct gttcctcgtg gggcacgtga cgaaggatgg gatggtcgcg
     1981 gggccgaagg tcctcgagca catggtcgat acggtgatct ctttcgaggg cgacgggagc
     2041 cacctgtacc ggatgctgcg ggtgatgaag aaccggttcg ggtcggtggc cgagatcggg
     2101 ctgttcgaga tgcagtcgcg cgggctggtg gaggtgacca atccctcgtc gctgtttctg
     2161 tcgcagcgcc aggatgcgcg ccggacgggg tcggtggtgg cgggcatctg cgaggggcag
     2221 cggccgcttt tggtcgagat ccaggcgctc gtgacggccg ccagctacgg caacccgcag
     2281 cgggtggccg ggggcatcga taacaaacgg ctggccctcc tgatcgcgat tctcga
//
LOCUS       DAOOKS010000297         2334 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_56915_length_2334_cov_3.000878, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000297 DAOOKS010000000
VERSION     DAOOKS010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56915_length_2334_cov_3.000878"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="PK112_05535"
     CDS             complement(<1..427)
                     /locus_tag="PK112_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010152232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HPC11544.1"
                     /translation="MLLHIPHSSDRIPAHLRAALLLSDDELRAELLALTDAHTDTLFT
                     LGVRPATPVIFPVSRLVVDPERFIDDSREPMAGKGMGVVYVRTADGRPLRTAPTAAQR
                     EQLLAAYYHPHHRRLTELVGLELAHLGRCLIIDCHSFPSR"
     gene            complement(435..959)
                     /locus_tag="PK112_05540"
     CDS             complement(435..959)
                     /locus_tag="PK112_05540"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HPC11545.1"
                     /translation="MMTKTIIAAGVCILACAGIWYWANRPFDPLPEGTVIDRVEVRKD
                     ERVMRLMKGGRSLREYRIALGRCPAGPKRCEGDGRTPEGGYTLDYRNPKSRFFLSYHI
                     SYPEPRDRRMADSLGCMPGGDIMIHGAAPGHAFFGRLYSYVDWTNGCIAITNPEMAEF
                     WRVVPVGTPITIYP"
     gene            complement(1036..>2334)
                     /locus_tag="PK112_05545"
     CDS             complement(1036..>2334)
                     /locus_tag="PK112_05545"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HPC11546.1"
                     /translation="SSCNVILLINESFGKRAFPTADSAVCPMPFLREWTRRESGRFFV
                     FPTAFTNSGVTDISVPSILSGVAPWEQSGTLHTMPLVWDWAAAAGYRTAFVSAQQFSW
                     ANLDDFFRSPGPDLYLGGDQTGRPLINDTGIDEIAAMQLFCDSVLANPARRPFCAVYN
                     SNALHTPGQTASDLLPDLTPAYSSRYCNSAAVLDRAIRLLCESLASHHLLDSTFIIIT
                     ADHGDTDSLIHSQVHRLYNFYDEIMNIPFLVYVPAAWLTDHPDRLAALRENEGRVVAN
                     LDILPTILAAIGANCDSANAGLRARLNGASLFAPLPPERNTVALNTNDIRQWEHEGFG
                     IFRRDRRFVYSDIEGPRWFDAADRNQTNDLWPTAPPGEKRRVLDVIDSIYHLRRMFPA
                     AAVSEADGVGAGGAFFDRAPSGLHFARASACGGLGPCSRE"
BASE COUNT          440 a          716 c          813 g          365 t
ORIGIN      
        1 gccgcgaggg aaaactgtgg caatcaatga tcaggcagcg tccgaggtgc gccagttcca
       61 ggccgacaag ctcggtgagc cggcgatggt gaggatggta gtaagcggcc agcaactgct
      121 cgcgctgggc ggcggtgggc gcagtacgca gcgggcggcc atcggccgtt ctcacataca
      181 cgacccccat ccccttcccc gccatcggct cccgcgagtc atcgataaac cgctccgggt
      241 cgaccacgag gcggctcacc gggaaaatca ccggcgtggc cgggcgaacg ccgagcgtga
      301 agagagtatc ggtgtgagca tcggtgagag ccaaaagctc ggcccggagc tcgtcatcgg
      361 acaacagcag cgccgcccgc aggtgagcgg gaatgcggtc cgaggagtgc gggatatgga
      421 gcaacatcgc agcctcaggg atagatggta atcggagtgc caaccgggac cacgcgccag
      481 aactcggcca tctccggatt cgtgatcgca atgcatccgt tggtccaatc cacatacgag
      541 tagaggcgcc cgaagaaggc atgaccgggc gcagcgccgt ggatcatgat gtccccgccg
      601 ggcatgcagc cgagcgagtc cgccatccgg cgatcgcgcg gctccgggta ggagatgtgg
      661 taggacagga agaaacggct cttcgggttc cgataatcga gcgtatatcc gccctccggg
      721 gtccggccgt ccccctcgca gcgcttcggc cctgcggggc agcggccgag cgcaatccga
      781 tactcccgca agctccgtcc acctttcatc agccgcatga cccgctcgtc tttgcggact
      841 tctaccctgt cgatcactgt tccctcgggc agcgggtcga aggggcggtt cgcccaatac
      901 cagatcccgg cacacgccag aatgcagact cccgcggcga ttatggtctt tgtcatcatg
      961 gctgtcgcgg gttgcctccg gatccggggc gaaaggtgcg gtcgttcgta tccgacatcc
     1021 ccacggcccg gcccatcact cccgactgca cggtcccaaa ccgccgcatg cggaggcgcg
     1081 ggcaaaatgc aggccgcttg gagcgcgatc gaagaacgcc ccgcctgctc cgacgccgtc
     1141 tgcctccgag acggcggcgg cggggaacat ccgcctgaga tggtaaatcg aatcgataac
     1201 gtccagcacg cgccgcttct cccccggcgg cgccgtcggc cagagatcgt tggtctggtt
     1261 gcgatcggcg gcgtcgaacc agcgcggacc ctcgatatcg gaatagacaa accgccggtc
     1321 tcgccggaaa atgccgaacc cctcatgctc ccactgccgg atatcgttgg tattgagagc
     1381 gaccgtattg cgctccggcg gaagcggcgc aaagagcgac gcgccgttca ggcgggcgcg
     1441 caggccggcg ttggccgagt cgcaattcgc cccgatcgcg gcgaggatgg tcgggagaat
     1501 gtcgagattg gcgaccaccc gcccctcgtt ctcgcgcagc gcggcaaggc ggtcggggtg
     1561 gtcggtcagc cacgccgccg ggacatagac gagaaacggg atattcatga tttcatcgta
     1621 gaaattatag agccggtgca cctgggagtg aatcaggctg tcggtgtcgc cgtggtccgc
     1681 ggtgatgatg atgaacgtgc tgtccagcag gtgatggctt gcgagcgact cgcagagaag
     1741 ccggatagcg cgatcgagca cggccgcgct gttgcagtag cgggaggaat aggccggagt
     1801 cagatcgggc agaaggtcgc tggcggtctg gccgggagtg tgcagggcgt tggaattgta
     1861 caccgcgcag aacggccgcc gggcggggtt ggccagcacg ctgtcgcaga aaagctgcat
     1921 ggcggcgatt tcatcgatcc cggtgtcgtt gatgagcggc cgaccggtct ggtccccgcc
     1981 gagatagagg tcgggaccgg gggaccggaa gaagtcatcg aggttggccc agctgaattg
     2041 ctgcgcgctc acgaaggccg tgcggtagcc ggccgcggcc gcccagtccc agaccagggg
     2101 catcgtgtgc agcgtgccgc tctgctccca gggggcgacg ccggagagga tcgagggcac
     2161 gctgatgtcg gtgacgccgg agttggtgaa ggcggtcggg aagacgaaga agcggccgga
     2221 ctctcgccgc gtccactcgc gaaggaaggg catcgggcag accgccgagt cggcggtcgg
     2281 aaacgcccgc ttgccgaacg attcgttgat cagcaggatg acgttgcagg agga
//
LOCUS       DAOOKS010000298         2330 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_57035_length_2330_cov_3.584615, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000298 DAOOKS010000000
VERSION     DAOOKS010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57035_length_2330_cov_3.584615"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="PK112_05550"
     CDS             <1..573
                     /locus_tag="PK112_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015188549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HPC11547.1"
                     /translation="PLLCAGLIGYRSLRMTGSARRLGLYGFGAAAHIVIQVARHQGRR
                     VYAFARRGDTASQDFARSLGADWAGPSDELPPEPLDAAIIFAPVGALVPLALQAVAPG
                     GVVVCAGIHMTDIPSFPYRWLWGERVVRSVANLTRRDGEEFLELAPRVPIRTEVHPYP
                     LNRANEALSDLREGNFTGAAVVVVDEDASG"
     gene            complement(996..1139)
                     /locus_tag="PK112_05555"
     CDS             complement(996..1139)
                     /locus_tag="PK112_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11548.1"
                     /translation="MPCNSIIVWEVRMLFLICPDSAGTPKVCTWVCSTLCRVKPCYGV
                     DPI"
     gene            1519..>2330
                     /locus_tag="PK112_05560"
     CDS             1519..>2330
                     /locus_tag="PK112_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003094817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HPC11549.1"
                     /translation="MSTFIASFIGELRNTHPGQPEFHQAVYDTLLSAAPALERTPAYQ
                     GANILGRIVEPERVVIFRVPWVDDGGRVRVNRGYRVEFSSALGPYKGGLRFHPGVNLG
                     VMKFLAFEQTLKNSLTTLPLGGAKGGSDFDTKGRSEGEVMRFCQSFMNELFRHLGPRI
                     DIIAGDIGVERREIGFLFGQYKRLRNAFDGAVMGKAVGWGGTVLRPEATGYGCAYFAA
                     EMLATRDESFAGKRVAISGAGTVALHAAEKVVALGGRVVAMSDSHGFVVDEAG"
BASE COUNT          361 a          781 c          785 g          403 t
ORIGIN      
        1 ccgctgctgt gtgccggcct gatcgggtac cgctctttgc gcatgaccgg ctctgcccgg
       61 cggctcggtc tgtacgggtt cggggcggcc gcgcacatcg ttatccaggt cgcccggcat
      121 cagggccggc gcgtgtatgc gttcgctcgc cgcggggaca ccgcaagcca ggattttgcc
      181 cgctcgctcg gggccgattg ggcggggccg tcggacgaac tccctccgga accccttgac
      241 gcggcgatta tcttcgctcc cgtcggcgcg cttgtgccgc tggccctgca ggcagtcgcc
      301 cccggcggcg tagtcgtgtg cgccggtatc cacatgaccg acatcccctc gttcccctac
      361 cgctggctgt ggggggagcg ggtcgtgcgc tcggttgcca atctcacccg ccgcgacggc
      421 gaggagtttc tggagctggc cccgcgcgtg ccgatccgga ccgaggtgca tccgtacccg
      481 ctcaaccggg ccaacgaggc cctcagcgac ctgcgcgagg ggaacttcac cggcgcggcg
      541 gtcgtcgtcg tggatgaaga cgcctcgggg tgagcgctct cggaactggc ggggaatgga
      601 caggcggagt atcggcgggg aggtccatac gcgaacctcc ccggacgaca cacaggaatc
      661 accatgggtc aggatcgacg ccgtagcacg gcttgatccc gcagtttgtg acgcagagcc
      721 aggtgcacac cttggccttg cacacgcact ccgggctgat caggtatacc ataggcacct
      781 cccatatcag gacgtttcgg taactcctgt ccggatggcc ggcgccgcag ccgccggctg
      841 gcggttccgg atctccggca ggtgtggacg ttcccatttt tgccgaccgg tcggcgggat
      901 aaatgtccgc gccgtcggcg accgtcatgc gacaatcggc gggtttgtcg caggggacac
      961 ttcgccgcgc gtatcccgtc cgcaaggcga tgcgactaaa tggggtcgac gccgtagcag
     1021 ggcttgaccc ggcacaacgt gctgcacacc cacgtgcaga ctttcggtgt gcccgcactg
     1081 tccgggcaga tcagaaagag catgcgcacc tcccatacga taatggagtt acagggcatc
     1141 ccgtccggtc gcggcggcgc cccagagcgt ccgtgcgctt atcgggcggg tgtgagcctc
     1201 atcctctgcc ggtcactgtc gtgggttccc gcgggcggcc ccagaatctc cgtcggcgct
     1261 gccataccgt ccgcggtgag gctgctcgag cgtcgccgta cacttatcgg tgccacccgg
     1321 cacccacggc ggcgcggcgc cccataagaa agaagaaaga tgggaatgcg gtgtcaagga
     1381 caaactagtc gatgaccggc cggcctgtcg ggcggccttg acaaagaccg ccggttctgc
     1441 tatatacccg tgattcgttt cccgaatgcc cgggctccgc gcggcggcga tccggatggc
     1501 gcccggcgag gtacatcgat gtccaccttc atcgcgtcgt tcatcggcga attgcgcaac
     1561 actcaccccg gccagcccga atttcaccaa gcggtgtacg acaccctgct gtcggccgcc
     1621 ccggcgctcg agcgcacgcc ggcgtaccag ggcgccaaca tcctcgggcg gatcgtggaa
     1681 cccgagcgcg tcgtcatttt ccgcgtgccg tgggtggacg acggcgggcg cgttcgggtc
     1741 aaccgcgggt accgcgtgga attctccagc gctctgggtc cctacaaagg cgggctgcgc
     1801 ttccacccgg gtgtcaacct cggcgtcatg aaattcctcg ccttcgagca gactctcaag
     1861 aacagcctca ccacgttgcc gctcggaggg gccaaggggg gatcggattt tgacacgaaa
     1921 ggccgctccg agggcgaggt gatgcggttc tgccagagct tcatgaacga gcttttccgc
     1981 cacctcgggc cccggatcga catcatcgcc ggcgacatcg gcgtggaacg ccgggagatc
     2041 ggcttcctct tcggccagta caagcggctg cgcaacgcgt tcgacggcgc ggtgatggga
     2101 aaggcggtgg gatggggcgg caccgtcctc cgcccggagg ccaccggcta cggctgcgcg
     2161 tatttcgccg ccgaaatgct cgccacccgg gacgaatcgt ttgcgggcaa gcgggtggcc
     2221 atctcggggg cgggcacggt ggctctccac gccgcggaga aggtcgtcgc cctcggcggc
     2281 cgcgtcgtgg ccatgtccga ctcccacggt ttcgtagtcg acgaggccgg
//
LOCUS       DAOOKS010000299         2330 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_57038_length_2330_cov_3.285275, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000299 DAOOKS010000000
VERSION     DAOOKS010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57038_length_2330_cov_3.285275"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..757
                     /locus_tag="PK112_05565"
     CDS             <1..757
                     /locus_tag="PK112_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HPC11550.1"
                     /translation="FRQHVATARRNRGVPMIPEFDEFPVFYFTNHRSIVGPGDVLVET
                     DHLVGLDFELECAVVIDRPGRNIRAEEADRWIAGYTIMNDFSARTLQMEEMKLNLGPA
                     KGKDFATAIGPWLVTPEELADRRLSTPGGAAYNLRMTARHNGRLVSEGNLRDMTWTFA
                     EIIERASYGVDLFPGDVIGSGTVGTGCYLELNGTWALAAAQRGESFTPVWLRAGDTIE
                     LEIERLGVLRNRIVKADRDYSILARKKTSAAAP"
     gene            774..1769
                     /locus_tag="PK112_05570"
     CDS             774..1769
                     /locus_tag="PK112_05570"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HPC11551.1"
                     /translation="MLHFEPKDLPSPQVHRYLLGGVGPRPIALVSTISPDGAVNLSPF
                     SFFNAFGANPPIVAFSPARRGRDGSLKDTYANLVATRECVVQAVTYAMVHQVSLASAD
                     YPPEVDEFVRSGLTPVPSDLVAPPRVKESPFQMECRLRHMLELGGLPGSGNLAVCEVV
                     KFHIAEDVIRDGVIVPDLIDLVGRQSADYYVRAAGAAVFAVPKPRGRGIGVDSLPAFI
                     RGSHLLSPSHLARLAGVEAIPDPDAAVCAVEQLAAPAPAETAGPGLEVSESAFFRFEQ
                     RGDYPAMLRAAVLLQRAGHPKAAAFIERAACAALDRGDVSFAWNALLYLGRLGRA"
     gene            1923..>2330
                     /locus_tag="PK112_05575"
     CDS             1923..>2330
                     /locus_tag="PK112_05575"
                     /inference="COORDINATES: protein motif:HMM:NF027503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-coenzyme A synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HPC11552.1"
                     /translation="MTAPAPSPLWVPPPSRVERSHLSRFMCFLGRRARREFFSYDDLW
                     QFSVDHIASFWEAVWDFAGIIHSQPWEAVVEGDRIDNAVWFRGTRLNFAENLLRYRDR
                     RTAIISECELGTVETITYADLYGRVARCAAGLKK"
BASE COUNT          325 a          979 c          651 g          375 t
ORIGIN      
        1 ctttcgccag catgtcgcca ccgcccgccg caaccgcggt gtccccatga tcccggagtt
       61 cgacgagttc cccgtcttct acttcaccaa tcaccgctcg atcgtcggcc ccggcgacgt
      121 cctcgtcgaa accgatcacc tcgtcggcct cgatttcgag ctggaatgcg cggtcgtcat
      181 cgaccgcccc ggccgcaaca tccgcgccga ggaggccgac cgctggatcg ccggttacac
      241 gatcatgaac gacttctccg cccgcaccct ccagatggag gagatgaaat tgaatctcgg
      301 ccccgccaag ggcaaggatt tcgccaccgc catcggcccc tggctggtca cccccgagga
      361 actggcggac cgccgcctct ccacccccgg cggcgccgct tacaacctcc gcatgaccgc
      421 ccgccacaac ggccggctcg tctccgaggg caacctcagg gacatgacct ggaccttcgc
      481 cgaaatcatc gagcgcgcct cctacggcgt cgacctcttc cccggcgatg tcatcggctc
      541 cggcaccgtc ggcaccggct gctacctcga gctcaacggc acctgggcgc tggcggccgc
      601 ccagcgcggc gagtctttca cccccgtctg gctgcgcgcc ggggacacga tcgaactcga
      661 gatcgagcgc ctcggcgtcc tgcgcaaccg catcgtgaaa gccgaccggg actactccat
      721 cctcgcgcgc aagaagactt cagccgcggc cccgtgaccg cccgcacacc ggtatgctcc
      781 atttcgaacc gaaagatctc ccttcgccac aggtccaccg ctacctgctg ggcggcgtcg
      841 gcccccgccc catcgccctc gtctccacca tctcccccga cggcgccgtc aacctctccc
      901 ccttctcgtt cttcaacgcc ttcggcgcca accccccgat cgtcgccttt tcccccgccc
      961 ggcgcggccg cgacggctcg ctcaaggata cctacgccaa cctcgtggcg acccgcgagt
     1021 gcgtcgtgca ggccgtcacc tacgccatgg tccaccaggt ctccctcgcc tccgccgact
     1081 acccgcccga ggtggatgaa ttcgtcagga gcggcctcac ccccgtcccc tccgacctcg
     1141 tcgccccgcc ccgggtgaaa gagtccccct tccagatgga gtgccggctg cggcacatgc
     1201 tcgagctggg cggcctcccg ggctcgggca acctcgccgt ctgcgaggtc gtcaagttcc
     1261 acatcgctga ggacgtcatc cgcgacggcg tcatcgtccc cgacctcatc gacctcgtcg
     1321 gccgccagag cgccgactac tacgtccgcg ccgccggcgc cgccgtcttc gcggtcccca
     1381 aaccccgggg gcgcggtatc ggcgtcgact ccctccccgc cttcatccgc ggctcgcacc
     1441 tcctctcccc cagccacctc gcgcgcctgg ccggggtcga ggcgattccc gaccccgacg
     1501 ccgccgtgtg cgcggtcgaa cagctcgccg cccccgctcc cgcggagacc gcgggccccg
     1561 gcctcgaggt ctccgagtcc gcgttcttcc gtttcgagca gcgcggcgac taccccgcga
     1621 tgctccgcgc cgccgtgctg ctccagcgcg ccggccaccc gaaagccgcg gctttcatcg
     1681 aacgcgccgc gtgcgccgct ctcgaccgcg gcgacgtctc cttcgcctgg aacgcgctcc
     1741 tctatctcgg ccgcctgggc cgcgcctagc cgccgccggc tccggccaaa aaatcgattg
     1801 atataatcca ttttccgtga acccgtcgcc cccgccgggt gttctgtcag gcggacgccg
     1861 ggcgcgcggc cgcgcccgcc cggccggcac tgaggagaac cgctgttgga gaacccgctc
     1921 ccatgaccgc ccctgcccca tcccccctct gggtcccccc gccgtcccgc gtcgagcgca
     1981 gccacctgtc ccgcttcatg tgcttcctcg gccgccgcgc gcgccgcgag tttttctcct
     2041 acgacgacct ctggcagttc tccgtcgacc acatcgcgtc cttctgggag gccgtctggg
     2101 acttcgccgg catcatccac tctcagccct gggaagcggt cgtcgagggc gaccgcatcg
     2161 acaacgccgt ctggttccgc ggcacccgcc tgaatttcgc cgagaacctc ctccgctacc
     2221 gcgaccggcg caccgccatc atctccgagt gcgagctggg gacggtcgag acgatcacct
     2281 acgccgacct ttacgggcgc gtcgcgcgct gcgccgccgg cctgaaaaag
//
LOCUS       DAOOKS010000300         2329 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_57073_length_2329_cov_3.464380, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000300 DAOOKS010000000
VERSION     DAOOKS010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57073_length_2329_cov_3.464380"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            839..2251
                     /locus_tag="PK112_05580"
     CDS             839..2251
                     /locus_tag="PK112_05580"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HPC11553.1"
                     /translation="MNTSRLTRAPLALPVPTLLLTLALLAVAAPRLTAAVTAPAAGVN
                     SITLTWTAPGDDGVSGRAAQYDVRYSLSPITNATWASASQATGEPAPAAAGSAEIFTV
                     TGLNSGTTYYFAVKTADEALNWSALSNVASGTTAVAGDASSPAAVTNLIVVAPTTTSL
                     TLVWTAPGDDGAVGTASRYDIRYSTLPISNGVTWNMATQLVSEPSPSPAGAAETLTVA
                     GLAEATTYYFALKTADEVPNWSTISNCPSGITAADAAPPAAVVDLRASSGDADSTIDV
                     VWTATGDDGLLGTAAAYEVRYSRTAISESNWSAATLWPSVITPLASGLEQRVTLTGLQ
                     PGEVYYVALKAYDDAANVSPLSNVDTGVAKYGIASDVDPDETLPGGYVLDQNFPNPFN
                     PTTSIRFSVPAAGRAELTVYNTAGQEVTRLVDGALAAGEHTVTWDGRDRTGYPVASGV
                     YYYRLAAGDQQHTKKMVLLK"
BASE COUNT          400 a          852 c          641 g          436 t
ORIGIN      
        1 agcgtcgccg actccagcgc cgcatcgatc ggcaacgcat acgaggccgt cagcggcatg
       61 ttggtctgcg gctccaccgg acccttgctg cagccggcaa ccaccagcgc cagcatgaac
      121 accggaacca gaaccttcat catcttgcgc attcgcatct ccttactcaa agcggtttgg
      181 ttgaatgatc ttgtgtctag gtaagtcgtt tactgtctgt tactttcaca cggcggaccg
      241 taaccgcagt ccgggccctc ttcacccgga gaacttctaa cccccgacgg ccaaccttga
      301 ccctgccact gcgcggtagc tgctcacgtt cttgggaagt ctgagacgat ccgtccctgg
      361 ctcgtcgttg ccctgccggc gccccgccgt ttatttcgtc tgccgattcc cggtgtggct
      421 ccgccgggac cttttccgtg tccgtctgcg gcgccgccgg ataagcagcc ggagagcgct
      481 ttgttgagac tttgtaatat aatgtcccga accgttttag gcaagcgaaa agatccatct
      541 cgcaggcgga aatactcctt ttagcctcgg tctaaagttc ccagaattag tcctaattct
      601 cctccaaacc gtatggagcg aatctcatgc cgatttccgc tgccgcccac ccttcctgcg
      661 cacattctgt gcaatgcact ggaatcaaac agaaaaatcc gtcaacccac ctctccggga
      721 tccctatcgg gcgtgcaaag tcttgccaca gtttggcttg caaattcact tcgggatggt
      781 ccggactttg catactctcc cccggtcagg caagcaagtc tttcgtttcg gaggtgagat
      841 gaacacgtcg cgacttaccc gcgcccctct ggcgctcccc gtacccacgc tgctccttac
      901 cctcgccctt ctggcggtgg cggccccgcg cctcacggcc gccgtgaccg cgcccgccgc
      961 cggcgtcaat tcgatcaccc tcacctggac cgcccccggc gatgacggcg tgtcaggccg
     1021 cgccgcgcag tacgatgtcc ggtactccct gtccccgatc acgaatgcca cctgggcctc
     1081 ggcatcgcag gcgaccggcg agccggcgcc ggcggccgcc gggtcggcgg agatcttcac
     1141 cgtcaccggc ctcaactccg gcactacgta ctattttgcg gtcaagaccg ctgatgaggc
     1201 gctcaactgg tcggccctgt cgaacgtcgc ctccgggacc accgcggtag cgggcgacgc
     1261 atcctccccg gccgccgtca ccaacctcat cgtggtcgcc cccaccacga cctcgctcac
     1321 gctggtctgg accgctcccg gcgacgatgg cgcggtcggc acggcctccc ggtatgacat
     1381 ccgctactcg actctgccga tctccaacgg cgtcacctgg aacatggcca cgcagctggt
     1441 ttccgagccg tcgccttccc cggccggcgc cgccgagacg ctgacggtcg ccggcctggc
     1501 cgaggccact acctactact tcgccctgaa gacggccgac gaggtgccca actggtcgac
     1561 gatctcaaac tgcccttccg gaatcaccgc cgccgacgcc gcgccgccgg ccgcagttgt
     1621 cgaccttcgc gcctcctccg gcgatgccga cagcacgatc gacgttgtct ggacggccac
     1681 gggcgacgat ggccttctgg gcacggcggc cgcgtacgaa gtccgctact cccgcactgc
     1741 catatccgag agcaactggt cggcggcgac cctctggccc tccgtcatca cccctctcgc
     1801 ctccggcctc gagcagcggg tcaccctcac cggccttcag cccggcgaag tctactatgt
     1861 cgccctgaag gcgtacgatg acgcggccaa cgtctcgccg ctctcgaatg tcgacaccgg
     1921 cgtcgccaaa tacggcatag cttccgacgt cgatcccgac gagactctcc ccggcggtta
     1981 cgtgctggac cagaacttcc cgaatccgtt caacccgacc acgtctatcc gtttcagtgt
     2041 gccggccgcc ggccgcgccg aactgaccgt ctacaacacc gccggccagg aggtgacccg
     2101 tctggttgac ggcgccctcg ccgccggcga gcatacggtg acatgggacg gccgcgatcg
     2161 cacgggatac ccggttgcct ccggcgtcta ctactaccgc ctcgccgccg gcgaccagca
     2221 gcacaccaag aaaatggttc tgctgaagta gggggatgag cttgagcgga gcctgcgttg
     2281 ccaacccagc agagaggatg atgcgcatat gagcataccg aaaccgagg
//
LOCUS       DAOOKS010000301         2327 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_57150_length_2327_cov_3.183539, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000301 DAOOKS010000000
VERSION     DAOOKS010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57150_length_2327_cov_3.183539"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(165..362)
                     /locus_tag="PK112_05585"
     CDS             complement(165..362)
                     /locus_tag="PK112_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11554.1"
                     /translation="MKETAKYFLWGVALSALLFLIDRLTGIDDLPYGWSLIAGIGVAI
                     LVGAVFAVVYRLIRRRRAKNG"
     gene            complement(1147..2235)
                     /locus_tag="PK112_05590"
     CDS             complement(1147..2235)
                     /locus_tag="PK112_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11555.1"
                     /translation="MRRWTRACGIMSAGSILAGLMLIVGLLGPACDDDPTGPDHPDLP
                     DGKDYVFYYRDQATGTLYGYHLVSERVDTFTVPYVARFGMFVSADGQRLYVAGPQATG
                     VVALATRTLVAELPYAQYPVGNGIIFSPDGRFMAFLGSTLTILTYPDHEPVFRDTMGA
                     GTGCFTPGGERLYYVPPGADQVRVVETTGGEARVHPRVFNPRLSKIRVLSDESRWVLY
                     QSISCDIGGVVSVYDPIRDTMEFQEWFYLSCGMLAVSPDDRWAFYDNPGPLLNPVPGT
                     PLEFRVLNLVTKRLDRLVSTVGIADGLNPQDMAVGWLYVSPDSRWLVAAGTWGDQFLV
                     FDVRHLKVDRYIKLDGFWSDMFAGQEGL"
BASE COUNT          475 a          787 c          656 g          409 t
ORIGIN      
        1 gaggaccaaa agcacgatgc cgatcaccat caggcccagt gcgactaatg aaatcatgat
       61 ttccgaacgc tcctattaga cgtctgtgcg atgccgccta atccaccgcc ccggtcgctc
      121 gcgcccgatg agaatgcgta cggggcgagc atccacgaga ctcgctaccc gttttttgcg
      181 cggcgccgcc ggatcagccg gtagacgact gcgaacacgg ctcccacgag aatggcgacg
      241 ccgattcctg ctatgagcga ccagccgtac ggtagatcgt cgatgccggt caggcggtcg
      301 ataagaaaca gcagagcgct gagagctaca ccccaaagaa agtacttggc tgtctctttc
      361 actggaggac ctcctttccc gatatttgcc tatctgcagt atggtaaccg gcgagtttgt
      421 acatcacgtc gatgtcatcc tccctttctt gcgcgggcgt cggcggcggg agccttgcgg
      481 ctcccgcctg atgagactgc gcagccggcc agtaccgact aaacgccgct acccgttttt
      541 tgcgcggcgc cgccgacaga gccgctggac agctccgaac gcgaccccca acacgctgat
      601 cggtcaccac gtgtccggtc ccccggggaa ggccggtgtt cccggtgcgt tgagtaggct
      661 cagcgggaac agccggcccg acccgtgtga ggaccatgcc tgcgacttct ctatcttgcg
      721 gtcggcggtt agcagatgcc tctggcctca ccccgatgca ctcatcgcct cctttctctc
      781 gttccccccg ggcccggggc ggttcggggg atgcagaata gatgagcgct tccaacccca
      841 agacagtgga atcgaaagta gcggtcgtca ggatgcacac aaggtacccg ccggagaacg
      901 gaatctcgtc aggcaggccg accacgccgt caagctgcgt gatcagcctg atcaacgagt
      961 ctacgggaat catcgaatcc ggcattacct ggacgcaccc gctgtcggta gccatttcga
     1021 ctggaccacc tcccgcgtag tagtgggtct ccccgaccgt cgtcgagcac agcgtggcca
     1081 cgactgcgcc tcacgcgagt aaatccctcc acctgtacat tgcagaacct cctgtgctcg
     1141 gaacgtctag agtccttcct ggccggcaaa catgtcggac cagaaaccgt ccagcttgat
     1201 gtaccgatct acctttaagt gcctcacatc gaaaaccaga aactgatccc cccaggtgcc
     1261 cgccgccacc aaccagcggc tgtcgggcga cacgtagagc caccccacgg ccatatcctg
     1321 cgggttgagc ccgtccgcta tccccaccgt gctgaccaac cgatcgagcc gtttggtcac
     1381 caagttaaga acccggaact caagtggagt gcccggcaca gggttcagca aaggccccgg
     1441 gttatcatag aacgcccagc gatcatcggg cgacactgcg agcatcccgc acgacaggta
     1501 gaaccactcc tgaaattcca tcgtgtcccg gatcgggtca tagaccgata ccacgccgcc
     1561 gatgtcgcag gagatactct ggtagagcac ccagcgggac tcgtcggaga gcactcgaat
     1621 cttgctgagg cggggattga acacccgggg atgcacccga gcctccccgc cggttgtctc
     1681 aacaacccgc acctggtccg cccccggcgg aacatagtac agccgctccc cgcccggcgt
     1741 gaaacacccg gtccccgcgc ccatcgtatc acgaaatacc ggctcgtgat ccgggtaggt
     1801 gaggatggtg agcgtactgc ccaggaaagc catgaaccgc ccgtccggcg aaaagatgat
     1861 gccgttgcct accgggtact gcgcatacgg cagttccgcc accagcgtcc tggtggcgag
     1921 cgccaccacc ccggtggcct gcggtccggc aacgtagagc ctttgcccgt cggccgacac
     1981 aaacatccca aaccgggcca cgtagggaac ggtgaacgta tccacacgct cgctgaccag
     2041 atggtacccg tacagggtcc cggtcgcctg atcccggtag tagaagacat agtccttgcc
     2101 gtccggcaaa tccgggtggt cggggccggt agggtcgtcg tcgcaggccg gccccagcag
     2161 cccgacaatg agcatcaatc cggccaggat gctcccggcc gacatgatcc cgcatgctcg
     2221 cgtccatcgc cgcatcgcgc tcctcctttg attcgtgtga ttgtagctct atgttagcac
     2281 gcgccgggcc atgccccctc aaacccttca aagaacagaa gcgagcg
//
LOCUS       DAOOKS010000302         2325 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_57238_length_2325_cov_2.224229, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000302 DAOOKS010000000
VERSION     DAOOKS010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57238_length_2325_cov_2.224229"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..204)
                     /locus_tag="PK112_05595"
                     /pseudo
     CDS             complement(1..204)
                     /locus_tag="PK112_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026071.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
     gene            complement(551..1849)
                     /gene="eno"
                     /locus_tag="PK112_05600"
     CDS             complement(551..1849)
                     /gene="eno"
                     /locus_tag="PK112_05600"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014262605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HPC11556.1"
                     /translation="MSDYEFIHARQIFDSRGTPTVEVDVILSDGTLGRAAVPSGASTG
                     AHEAVELRDGDKSKYLGKGVQKAVDNVNEKIAPALLKDGIDPYDQVAVDNYLIGLDGT
                     ENKGKLGANALLGVSLATAKAAAESRGRFLYEYIGGCNCKLLPVPMMNIINGGKHADN
                     NVDLQEFMIMPVGAGSLSEALRMGSEVFHHLKKVLAAKKYNTAVGDEGGFAPDLKSNE
                     EALQVIMEAIAKAGYQPGEQIRIALDPASTEFYDAKSGKYNLKAEGKKLSSSEMIDFY
                     ERLCDKYPIISIEDGLAEDDWKGWKEMTERLGSRIQLVGDDLFVTNIKRLGRGIAEGI
                     ANSILIKLNQIGTLTETLDAIRMAQHAGYTAVVSHRSGETEDATIADAVVATSAGQIK
                     TGSMSRTDRIAKYNQLLRIEEQIGKYAEYPGMNAFSSVRR"
BASE COUNT          365 a          817 c          676 g          467 t
ORIGIN      
        1 gccctccagc acgcggagga ggtccgcgtc tccggtctcg gccaccacgt cggccgcgcc
       61 tgcatagagg tacgtggagc gcaccgcatg accggcgatc accgtctggt cgcgcagtgg
      121 cacccggtcc tggttgtcgt cgccgcccag gccactcgtc gcgacccggt cacgaatgtc
      181 gagcagggcc ttggcgagat cgactaccgc cgctccgggt tcctcgaact gggcggcatc
      241 taggctcttg agcgaaagtc cgcgtgcaat ccgcggtcgg ggggaaaaca atatgggtgg
      301 ccaccttctc cagggcaggg ttgatcgcaa tggctctttc tcttgccact acggcggtgg
      361 gccacccatt tgtcgcgaac gtctcgacag tgagcgatcg cgctcctgcc cgcgcgccat
      421 cgctcacccc cgcgaaacgt ccgcccatca ggcccaggcg atttcgacgg cgcctggtcc
      481 gccgcgcggc cgggacgccg gccgccgcgt gtgtcggtct ccccggaacc cccccgcttc
      541 ggcgctcccg ctacctccgc acgctcgaaa aggcgttcat tcccgggtac tccgcatatt
      601 tgccgatctg ctcctcgatc cggaggagct ggttgtactt cgcgatccgg tcggtccgcg
      661 acatcgaccc cgtcttgatc tgccccgcgc tggtcgccac caccgcgtcc gcgatcgtcg
      721 cgtcttccgt ctcccccgac cggtgcgaca cgaccgccgt ataccccgcg tgctgcgcca
      781 tgcggatcgc gtcgagcgtc tcagtcagcg ttccgatctg gttcagcttg atgagaatcg
      841 aattggcgat cccctccgcg atcccccgcc cgagccgctt gatgttcgtc acgaacagat
      901 cgtcgccgac cagctggatc cggctcccca gccgctcggt catctctttc caccccttcc
      961 agtcgtcctc cgccagcccg tcctcgatcg agatgatcgg gtacttgtcg cacaggcgtt
     1021 cgtagaagtc gatcatttcg ctcgacgaga gctttttccc ctccgccttg aggttgtact
     1081 tgcccgactt ggcgtcgtag aactcggtcg aggccgggtc gagggcgatc cggatctgtt
     1141 cgccgggctg gtagccggct ttggcgatcg cctccatgat cacctggagc gcttcctcgt
     1201 tggacttgag atccggagcg aagccccctt cgtcccccac cgccgtgttg tacttcttgg
     1261 cggccagcac ttttttcagg tggtggaaca cctcggagcc catgcggagc gcctcgctga
     1321 gactccccgc gcccaccggc atgatcatga actcctgcag gtcgacattg ttgtccgcgt
     1381 gcttgccccc gttgatgatg ttcatcatcg gcaccggcag gagcttgcag ttgcagcccc
     1441 cgatgtactc gtagagaaac cggccgcgcg actcggccgc cgctttcgcc gtcgccagcg
     1501 acacgccgag caaggcattg gccccgagct tccccttgtt ctcggtcccg tcgagcccaa
     1561 tcaggtagtt gtcgaccgcc acctgatcgt acggatcgat cccgtctttg agcagcgccg
     1621 gggcgatctt ctcgttgacg ttgtcgaccg ccttctgcac ccctttgccg agatacttgc
     1681 ttttatcgcc atcgcgcagt tcgaccgctt cgtgcgcccc ggtcgaggcc cccgacggca
     1741 cggcggcccg cccgagcgtg ccgtccgaaa ggatcacatc gacctccacc gtgggcgtgc
     1801 cgcgtgagtc gaaaatctgt cgcgcatgaa tgaattcgta gtccgacatg gagaaacctc
     1861 tcgtcagcca attgccgttt cacatccgcc gtagatgcta ccatccgccc cggacgcatg
     1921 caagttttaa cagtcattgg ggggacaagc cctcagagta tggggcccgg ccgcgacttc
     1981 cgcgctgtgg ccggtcccga tccctttgtg cggcaacacg cttggcgccg gcacccggtt
     2041 tgatacgtag gccgtgtacc ttgggtagga cagcaagacc ggcggattaa aagatgccgg
     2101 tcggggccgg ccgctaaccg gccccttttt tattccctca ccaaatgtga ttgtgctctc
     2161 ccccgccacg ggtagtttct ggcttagcgc ggcccgaaac agccggcgga tggcggtttt
     2221 cgactcgcgc taaagaaaaa actcgacaaa ccggtgcaaa agcgcgttaa acccaatctg
     2281 aacacgaggc cgacgcgagc cgcctgcccc cgcgggaaac ggtcg
//
LOCUS       DAOOKS010000303         2324 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_57270_length_2324_cov_3.074041, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000303 DAOOKS010000000
VERSION     DAOOKS010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57270_length_2324_cov_3.074041"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..920)
                     /locus_tag="PK112_05605"
     CDS             complement(72..920)
                     /locus_tag="PK112_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11557.1"
                     /translation="MRSHRSLFLSGAALALAALAFIWGCSDDDDNPTKWGSLEDPQFQ
                     AANTQFEALLDTTITQFTEALGAIQTLPANDDDPIHHGPGDPNGITDSLIVSYSSGWH
                     TIHLEWHSQSGYIAIVNDSIQFLRNGQPTQEGSGLEQLLYRHRWSWTAPDTTVTYING
                     AGYSLFDFDGLHTQEATIDGANGCSVETKFVFADSVVHYDFAAEAALDDVVVTKTGPT
                     WDRGCPTSGRFTATIMMTYQRNDEAPVPTQWNFSATFHNGAVHVVATANGYTWTYDGV
                     ACSQEG"
     gene            complement(973..1875)
                     /gene="argF"
                     /locus_tag="PK112_05610"
     CDS             complement(973..1875)
                     /gene="argF"
                     /locus_tag="PK112_05610"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HPC11558.1"
                     /translation="MARSLTQITDLTADEVRRIFDLCARMKSGQIAPKPLAGKAVACI
                     FTKPSLRTRISFEVGINQLGGHALYITDNEIKLGERETIQDAARVLSRYIGMIMIRTF
                     KQSDVEGLAKYASVPVVNGLTDLVHPCQILGDYFTVMESFPGRERFKIAYVGDGNNIV
                     NSWLNLASLIPMDLRIGTAADTQPDGAILRRAQANPASRVLLTEDPKEAVAGADVVYT
                     DVWASMGQKHLAEQKAEKLKKYQLNSSLLALADPKAIVLHCLPAERGREITDEVIDGP
                     HSRVFDEAENRLHIQKAIMTFLLQ"
     gene            2088..>2324
                     /locus_tag="PK112_05615"
     CDS             2088..>2324
                     /locus_tag="PK112_05615"
                     /inference="COORDINATES: protein motif:HMM:NF039187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HPC11559.1"
                     /translation="MTGRRATFGIILILLGFLFLLNSLDVMSVGEVLRYLVPLGFIAL
                     GIWLIIRRKQQDAALSFEAASAPPPPPPPSPSPAG"
BASE COUNT          378 a          666 c          811 g          469 t
ORIGIN      
        1 cgaggcagaa aaagttcgcg cccggctgcg gtcggcggcc gggcgcggaa attgagagta
       61 cgcagcgggc gtcagccttc ctgcgagcag gcgacgccgt cgtacgtcca ggtgtacccg
      121 ttggcggtgg cgacgacgtg cacggcgccg ttgtggaatg tcgcgctgaa attccactga
      181 gtcggcaccg gcgcttcgtc gttgcgctgg taggtcatca tgatggtcgc ggtgaaccgg
      241 ccggaggtcg ggcagccgcg gtcccaggtg gggccggttt tggtcacgac gacgtcgtcg
      301 agcgccgctt ccgcggcgaa gtcgtagtgc acgaccgagt cggcaaagac gaacttggtc
      361 tcgacgctgc agccgttggc gccgtcgatg gtcgcttcct gcgtgtggag tccgtcaaaa
      421 tcgaacaggc tgtagcccgc gccgttgatg taggtgacgg tggtgtcggg agcggtccac
      481 gaccaccggt ggcggtagag gagctgctcg aggccggagc cctcctgggt cggctggccg
      541 ttgcgcagga actggatcga gtcgttgacg atggcgatgt agccgctctg gctgtgccat
      601 tccaggtgga tggtgtgcca gccggagctg taggagacga tcagggagtc ggtgataccg
      661 ttcggatcgc cgggaccatg gtgaatcgga tcgtcgtcgt tggcgggcag ggtctgaatc
      721 gccccgaggg cctcggtgaa ctgggtaatg gtggtgtcga gcagcgcctc aaactgggta
      781 ttggccgcct ggaactgggg atcctcgagg ctcccccact tggtcgggtt gtcgtcgtcg
      841 tcgctgcagc cccagatgaa agccagcgcg gccagagcca gcgccgcgcc cgagaggaac
      901 aaagacctgt gagaacgcat ctgcgtctcc tttctctgca gacagctcct cccgggcggc
      961 cggccgaaga ggctattgga gcaggaaggt catgatggcc ttctgaatgt gcagcctgtt
     1021 ctccgcttcg tcgaagaccc gcgagtgcgg tccgtcgatc acttcgtcgg tgatctcgcg
     1081 tccccgctcg gcggggaggc agtgcaacac gatagctttc ggatcggcca gcgccagcag
     1141 ggagctgttg agttggtatt ttttcagctt ctctgctttc tgctcggcca gatgtttctg
     1201 ccccatcgag gcccaaacat cggtatagac tacatcggcc ccggcgaccg cttctttagg
     1261 gtcctcggtg aggagaaccc ggctggccgg gtttgcctgg gcgcgccgga gaatagcccc
     1321 gtcgggctgg gtgtcggcgg ccgtaccgat ccgcaaatcc atgggaatca gcgaggccag
     1381 gttgagccag ctgttgacga tgttgttgcc gtcgcccacg taggcgatct tgaagcgctc
     1441 ccgcccgggg aagctctcca tgacggtgaa gtagtcgccg agaatctggc agggatgcac
     1501 gaggtcggtc aggccgttga cgacgggcac ggacgcgtac ttggcgaggc cctcgacgtc
     1561 ggactgcttg aacgtgcgga tcatgatcat gccgatatag cgggagagca cccgggcggc
     1621 gtcctgaatc gtctcgcgtt cacccagctt gatctcgttg tcggtaatgt agagggcgtg
     1681 gccgcccagc tggttgatgc cgacttcgaa actgatgcgg gtgcgcagcg acggtttggt
     1741 gaaaatacag gcgaccgctt tgccggcgag cggcttgggg gcgatctgcc ccgatttcat
     1801 ccgggcgcac aggtcgaaaa tccggcggac ctcgtcggcc gtcaggtcgg taatctgcgt
     1861 gagcgagcgg gccatggcgt ctccttatgc ggtagtgtca tttttccgtg ttcagtcgtg
     1921 ttttggggaa taaagaggat ggttggggcg atgtcaagtc ggggggggcc ggacagccgt
     1981 cgtcaccccg ggctgcccga caaagcccgc cgaatccctt gccaaccgga accgggcgac
     2041 cgatattccg taatgaactg tggggatgag aaaggacgtt gacgacgatg accggacggc
     2101 gcgcgacatt tggcattatc ctcatccttc tggggttcct ctttctgttg aattcgctgg
     2161 acgtgatgtc ggtcggggag gtgctccgct acctcgttcc gcttgggttc attgcgctgg
     2221 gaatctggct gatcatccgg cgcaagcagc aggatgcggc gctgagtttc gaagcggcgt
     2281 cggcccctcc tcccccgccc ccgccctcgc cctctccggc cgga
//
LOCUS       DAOOKS010000304         2321 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_57390_length_2321_cov_2.976611, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000304 DAOOKS010000000
VERSION     DAOOKS010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57390_length_2321_cov_2.976611"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..1775)
                     /locus_tag="PK112_05620"
     CDS             complement(135..1775)
                     /locus_tag="PK112_05620"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPC11560.1"
                     /translation="MKSRSLIYNANILTQDRRLAVDSMAIERGRIVAVGDNLRGVGPF
                     AGFDRIDLRRRTVVPGFVDAHTHFYYYALSLGRIDLHGVDSLDECLRLIRGFCRGLGP
                     DDWVVGEGYRPDLFAGRVEPDRRQLDRVVGRRPAFIFSHDQHSAWVSTRALELAGLTR
                     RTPDPAGGVIERDPDGSPRGLLREIPGYDPVFRLIPAPSRPLLDRLYGRALAQAYRRG
                     VTGVHSFDGPAGFAWFIERAARGALGLRINYYAPAPGLPELRAAGTRYGAGDDFLRVA
                     GIKIFADGSLGSRTALCYQPYRDDRRNRGVEVTPPGKIASLARAAARLGLPCAIHAIG
                     DRAVANVLDALAAAPPLRPPARHRIEHLQLVRRRDLPRLKKLNIVASVQPQQTVSDIE
                     MVRAAWGGRAKDAYIFRALLDLGIDLAFGSDVPIEPLDPLAGIAAAVRRAHGPGEAFP
                     DSGPAGKRARRGAGRDVFHPEQRITAAEALFAFTAGPAIAAGQAECRGRLLPGYPADF
                     VILSQDPTRVAPLRLYDTEILATVLDGKVKYTAQGFSL"
     gene            complement(1750..>2321)
                     /locus_tag="PK112_05625"
     CDS             complement(1750..>2321)
                     /locus_tag="PK112_05625"
                     /inference="COORDINATES: protein motif:HMM:NF020672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SelB C-terminal domain-containing protein"
                     /protein_id="HPC11561.1"
                     /translation="RRLEKHFERKSHLKGVPIETLQQLSPYGRETTDLLIQYALENRI
                     LVRHDDEYSVAGRGMTLAGPVRTAYDEIMAALRADPWAPPKLADLTSRGKAHREAIRF
                     ILDSREGHKCGADFIFLADTWQTVLAYIRERLDRTGELAITDLRDRFGFTRKFAVPIL
                     EETDRLRLTRREGDLRVKGERYEVPLADL"
BASE COUNT          366 a          687 c          942 g          326 t
ORIGIN      
        1 tcgcggtctg aggggggaga tccacaaccg caggggggcg gctgttcgtg gtcccaaccg
       61 aatggactaa aggggagcac cgtgcggcat tgtccgcggc gcgcttgagc ggccgaaggc
      121 ggctgttatc ttccctacaa tgaaaacccc tgcgccgtgt atttcacctt tccgtccagc
      181 acggtcgcga ggatctcggt gtcgtagagg cgcagcgggg cgacgcgggt ggggtcctgc
      241 gagaggatga cgaagtcggc cgggtagccg gggaggaggc ggccgcggca ttcggcctgg
      301 ccggcggcga tagccgggcc ggcggtgaag gcaaagagcg cctcggccgc ggtgatccgc
      361 tgctccggat gaaagacgtc acggccagcg ccgcgccggg cccgctttcc ggcgggaccg
      421 gaatcgggga aggcctcgcc gggtccgtgg gcgcggcgga cggcggcggc gattccggcg
      481 agcggatcga gcggctcaat cggcacgtcc gagccgaacg ccagatcaat gccgagatcg
      541 agcagggcgc ggaagatgta agcgtccttg gcgcgcccgc cccaggcggc gcggaccatc
      601 tcgatgtcgg aaaccgtctg ctgcggctgc acggaggcga caatattcaa tttcttcagg
      661 cgcgggagat cccggcggcg gaccagttgg agatgttcga tgcggtggcg ggcgggcggg
      721 cggaggggcg gggcggccgc gagcgcgtcg aggacattcg cgacggcgcg gtcgccgatg
      781 gcgtggatgg cgcagggaag gccgaggcgg gcggccgcgc gcgcaagcga agcgatcttg
      841 cccggcgggg tgacctcgac gccgcggttc cgccggtcgt cgcggtacgg ctgatagcac
      901 agggcggtcc ggctccccag ggaaccatcg gcgaaaatct tgatgccggc gacgcggagg
      961 aagtcatcgc cggcgccgta gcgggtgccg gcagcgcgca gttcgggcag gcccggggcg
     1021 ggggcgtagt agttgatgcg gaggccgagc gcaccgcggg cggcgcgttc gatgaaccag
     1081 gcgaaccccg ccggaccatc gaacgagtgc acgccggtga cgccccggcg gtaggcctgc
     1141 gccagggccc gcccatagag gcggtcgagg agcggccgcg agggcgcggg tatgagccgg
     1201 aacaccgggt cgtagcccgg gatctcgcgg aggaggccgc gcggagaacc atcggggtcg
     1261 cgctcgatga ctccgccggc cggatccggc gtgcgccggg tgaggccggc gagttccagc
     1321 gcccgcgtgc tcacccaggc cgagtgctgg tcatgcgaga aaatgaacgc cgggcggcgg
     1381 ccgaccactc ggtcgagctg gcggcggtcc ggttcgactc gtccggcgaa gagatcgggg
     1441 cggtagccct caccgaccac ccagtcatcg gggccaaggc cgcggcagaa gccacggatg
     1501 aggcgaaggc actcgtccag ggaatcgacg ccgtggaggt cgatgcgccc gagggagagg
     1561 gcgtagtagt agaagtgggt gtgggcgtcg acaaagccgg gcacgacggt ccggcgcctg
     1621 aggtcgatgc ggtcgaaacc ggcgaaaggg ccgacgccgc ggaggttgtc gccgacggcg
     1681 acgatccgcc cacgctcgat cgccatcgaa tcgaccgcga ggcggcggtc ctgagtcagg
     1741 atgttggcgt tatagatcag cgagcgggac ttcatagcgt tctcctttga cgcggaggtc
     1801 gccctcgcgg cgggtgagcc ggaggcgatc ggtttcctcg aggatgggca cggcgaattt
     1861 gcgggtgaag ccgaagcggt cgcggaggtc ggtgatggcg agttcgccgg tccggtcgag
     1921 ccgttcgcgg atgtaggcga ggacggtctg ccaggtgtcg gcgaggaaga tgaaatcggc
     1981 gccgcacttg tgcccctcgc gggagtcgag gatgaagcgg atcgcctcgc ggtgcgcctt
     2041 gccgcgggag gtcagatcgg cgagtttagg cggcgcccag gggtcggccc ggagtgcggc
     2101 catgatctcg tcgtaggcgg tgcggacggg gccggcgaga gtcatgccgc gcccggcgac
     2161 ggagtactcg tcgtcgtgcc gcaccaggat ccggttctcc agggcgtact ggatgaggag
     2221 gtcggtcgtc tcgcggccgt agggggagag ctgctggagc gtctcgatcg ggactccctt
     2281 gaggtgggac ttgcgctcga agtgtttctc gagacgtcgc t
//
LOCUS       DAOOKS010000305         2321 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_57394_length_2321_cov_2.750221, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000305 DAOOKS010000000
VERSION     DAOOKS010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57394_length_2321_cov_2.750221"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /locus_tag="PK112_05630"
     CDS             complement(<1..774)
                     /locus_tag="PK112_05630"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPC11562.1"
                     /translation="MSDFHFFPEQVVWEITFACNMRCIHCGTSAGVPRSDELTTDEAL
                     RLIDELTGLGARGITLSGGEPLLRKDWPVLGKRIVANGAGAYLITNGFAVDEQAVDLF
                     QEVGLRNVGVSFDGSKEVHNYIRQRKTSYDKALHAMRLMAERKMRFEAVSQISKVNLK
                     DMDSIHKDLVSIGCPEWRIQMTTVTGRMEKEMVMGLAEYEQLIDKILEFKKDTRMMVD
                     VGENIGYYGCKGTQLLDGMPYLGCYAGVRVAGICSNGDVK"
     gene            complement(771..2150)
                     /locus_tag="PK112_05635"
     CDS             complement(771..2150)
                     /locus_tag="PK112_05635"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPC11563.1"
                     /translation="MPTLSRYNHFQPWQDGYYIAYNALSGAVALMTEENYAVYRRLVG
                     KLNGAATPDLSEEEAGLLKQLEYGAFVRSDGVDERALLRFNHNIARYDRTTLGFVIAP
                     TMACNMACVYCFENKTARKMSPEVMAAIVSYVEKRARSISGLQIDWYGGEPLLVLDVI
                     ESLTASFVEMGEKHQFEYSSSMISNGYLLTAETVDRLVALKIRHVQITLDGPARIHNV
                     KRPLKNKKESFYRILENLKYASTKLSVGIRVNVDKSFTIDMIEELLAELTAAGLRERA
                     GVYFGMVEPSSQVCANIAESCYETADFSAIEVDYYRLLLARGFRIDKLPSPVAVFCMA
                     QSTNAHLVDPDGNLYRCWNHAGDAPKAYGTITDEINYLHPNFQRLFSADPFDHALCVE
                     CELLPICMGGCPARRADRQLAGTQMCEAWKHNLQPMLEIIARSKQQEMARRQQQEAEA
                     QAAEKESTT"
BASE COUNT          386 a          726 c          734 g          475 t
ORIGIN      
        1 cttgacgtcg ccgttggagc agatgccggc cacccggacg cccgcgtagc agcccaggta
       61 gggcatgccg tcgaggagct gcgtcccctt gcagccgtag tagccgatgt tctcgccgac
      121 gtcgaccatc atgcgggtgt ctttcttgaa ctcgagaatc ttgtcgatca actgctcgta
      181 ctcggccagg cccatgacca tctctttctc catgcggccg gtgacggtgg tcatctgaat
      241 acgccactcg gggcatccga tggagacgag gtccttgtgg atgctgtcca tgtccttcaa
      301 attgaccttg gagatctggc tgacggcctc gaagcgcatc tttctttcgg ccatgaggcg
      361 catggcgtgg agcgctttgt cgtagctggt tttgcgctgg cggatgtagt tgtgcacctc
      421 tttggagccg tcgaacgaca cgccgacgtt gcgcaggccg acctcttgga acagatcgac
      481 ggcctgttcg tcgacggcga aaccgttggt gatgaggtag gcgcccgcgc cgttggcgac
      541 gatacgcttg ccgagcaccg gccagtcttt gcgcagaagc ggttcgccgc cggagagggt
      601 gatgccgcgc gccccgaggc cggtcagttc gtcgatcagc cgcagggcct catcggtcgt
      661 cagctcgtcg ctgcgcggga cacccgccga ggtgccgcag tggatgcagc gcatgttgca
      721 ggcgaacgtg atctcccaga ccacctgttc ggggaaaaaa tgaaaatcgc tcatgttgtc
      781 gactccttct cggccgcttg cgcttccgct tcttgctgct gccggcgcgc catctcctgc
      841 tgcttggagc gggcgataat ctcgagcatc ggctgcaggt tgtgtttcca ggcctcgcac
      901 atctgcgtgc ccgcgagctg gcggtcggcc cgccgggccg gacacccgcc catgcagatc
      961 gggagcagtt cgcattccac acacagggcg tggtcaaacg gatctgcgga aaacagacgc
     1021 tggaaattgg gatgcagata attgatctcg tcggtaatcg tgccgtacgc cttgggggca
     1081 tcgccggcgt ggttccagca ccggtacaga ttcccgtcgg gatcgaccag gtgcgcgttc
     1141 gtcgactggg ccatgcagaa caccgccacc ggcgacggca gcttgtcgat ccggaacccc
     1201 cgggcgagca gcaaccggta gtagtccacc tcgatcgccg agaaatcggc cgtctcgtag
     1261 catgattcgg cgatattcgc gcagacctgc gaggacggct cgaccatgcc gaagtagacg
     1321 ccggcccgct cccggagacc ggccgccgtc agctccgcga ggagctcttc gatcatgtcg
     1381 atcgtgaagg acttgtcgac attcacccgg atgccgacgc tcagtttggt ggaggcgtat
     1441 ttcaggttct cgagaatccg gtagaaactt tccttcttgt tcttcagcgg gcgcttgacg
     1501 ttgtgaatcc gggcagggcc gtcaagcgtg atctgcacgt ggcggatctt caaggccacg
     1561 agccggtcga ccgtctccgc cgtcaacagg tagccgttgc tgatcatgga cgagctgtac
     1621 tcgaattggt gcttctcgcc catttccaca aacgacgcgg tgaggctctc aatcacatcg
     1681 agaaccagca gcggctcgcc gccgtaccag tcgatctgga gaccgctgat cgagcgggcc
     1741 cgcttttcga cgtagctgac gatcgccgcc atcacctcgg gggacatttt ccgggcggtc
     1801 ttgttctcga agcagtacac gcacgccatg ttgcaggcca tggtcggggc gatgacgaac
     1861 ccgagcgtgg tccggtcgta gcgggcaatg ttgtgattga accgcaggag cgcccgttcg
     1921 tcgactccgt cggagcggac aaacgcgccg tactccagct gcttgagcag accggcctcc
     1981 tcctcgctca ggtcgggggt cgccgcgccg ttgagcttcc cgacgagccg gcggtagacg
     2041 gcgtagttct cctcggtcat cagcgccaca gctccggaca gggcgttgta ggcgatgtag
     2101 tagccatcct gccacggttg gaagtgatta tagcgcgaga gtgtcggcat cttccgtttc
     2161 catcatttgc gtctccgcgc gatggggcag tgcccggttc tgccccacag gcggttgttc
     2221 cggccgcagc tgttcagccc gcggccgcac ctcatgtcgg cgtctgttcc gcgacgccct
     2281 ctgccccgca gcccggaaac ccgggctgcg ggcaattcgg t
//
LOCUS       DAOOKS010000306         2320 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_57431_length_2320_cov_2.685651, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000306 DAOOKS010000000
VERSION     DAOOKS010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57431_length_2320_cov_2.685651"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="PK112_05640"
     CDS             <1..592
                     /locus_tag="PK112_05640"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: protein motif:HMM:NF003915.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HPC11564.1"
                     /translation="AASDGSDIGLSSADAAAADLQRHGLGPGDFVIGLAASVRTPYTR
                     AGLEYARSLGAKTAFLICNDPVNLPPGLDVVIALPVGPEVIAGSTRMKSGTAQKMALN
                     MISTAAMVLLGKTYGNLMVDLQARSEKLAARSRKILIDLLRLEYEEADRLLEAAGGSV
                     KTAIVMQKLKCTAAEARALLARHGGFVRNVLQQENP"
     gene            610..1146
                     /locus_tag="PK112_05645"
     CDS             610..1146
                     /locus_tag="PK112_05645"
                     /inference="COORDINATES: protein motif:HMM:NF025734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding domain-containing protein"
                     /protein_id="HPC11565.1"
                     /translation="MSTIRQFRIGFALAAAAAVLMTYAAMVAGSDAGEPADPRDTRRA
                     RSDASGSIPDPVINRVNEQFRTVEPILRASCYDCHSAYTEYPWYHRLPVISGMIDDHI
                     KEGREHLDFTDGFPFAGDADQVPLLQKMRKEIDRGDMPPLSYRLMHWGAKIDGAERDS
                     VFAWIDRTVLFLEQSRGQ"
     gene            1367..>2320
                     /locus_tag="PK112_05650"
     CDS             1367..>2320
                     /locus_tag="PK112_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11566.1"
                     /translation="MPRTALTLVPLMILALLVFGCSDNPADTAATNSADLNDFFGGYT
                     ATPEPPGFGDPSLLAGAGSEVSYDDPILASPAADSIVGDPEAGFFSLRVVWGRLRYDS
                     TVTTPTDWTGSLAVTRGVEIVRRVIAFEPAQDYVLERTDPKLVAWVSRTTVHHDGVAI
                     DIFVPRPHAVVDTVEIPIVDTLGDTTWQTVIDTFPSEPVEVTFATGPYTRTFTLGELV
                     ALDTIVYLDDSNAVAFHAFRMDRVPCARGFLAGFWGVDEEGNNEFRGMWMSRFGYLEG
                     WLQGTYGRNDAGENVFVGKWVDDRGNFEGFLRGTWMPHPDRH"
BASE COUNT          393 a          782 c          738 g          407 t
ORIGIN      
        1 cgctgccagc gacggcagcg acatcgggct atccagcgcc gacgctgcag cggccgatct
       61 gcagcgccac gggctcgggc cgggggattt tgtcatcggc ctcgccgcct ccgtgcgcac
      121 cccgtacacc cgggcggggc tggagtatgc ccgcagcctg ggcgcgaaaa cggccttcct
      181 catctgcaac gatccggtca atctgccgcc cggtctcgat gtcgtcattg cgctgccggt
      241 ggggccggaa gtgatcgcgg gttcgacgcg catgaaatcc ggcacggcgc agaagatggc
      301 gctcaacatg atctcgaccg cggccatggt gctgctggga aaaacgtacg gcaacctcat
      361 ggtcgatctg caggcccgct cggagaagct cgcagcccgg tcgcgcaaga tcctgatcga
      421 tctgctccgg ctcgagtatg aggaggccga ccggctcctc gaggcggccg gcggatcggt
      481 caaaacggcc atcgtgatgc agaagttgaa atgtacggca gccgaggcgc gcgccctgct
      541 ggcccgccac ggcggcttcg tgagaaacgt cctgcaacag gagaatccct aaaccgagga
      601 tgcagagccg tgtccacgat acgccaattc cggatcggct tcgctctcgc cgcggcggcg
      661 gccgtgctca tgacctacgc cgccatggtc gccggcagtg acgccgggga gccggccgac
      721 ccgcgtgaca cccgccgcgc gcgcagcgac gcctccggca gcattccgga tccggtgatc
      781 aaccgggtaa acgaacagtt ccgcaccgtc gaaccgatcc tccgcgcttc ctgctatgac
      841 tgccacagcg cctacacgga atacccctgg taccaccgcc tcccggtgat ctcgggcatg
      901 atcgacgacc acatcaagga ggggcgggag cacctcgact tcaccgacgg tttccccttt
      961 gcgggcgatg ccgaccaggt gccgttgctg cagaagatgc gcaaggagat cgaccgggga
     1021 gacatgcccc ccctgagcta ccggctgatg cactgggggg ccaagatcga cggggcggag
     1081 cgcgactctg tgtttgcgtg gatcgatcgc acagtcctgt ttctggaaca gagccgcggc
     1141 cagtgacgca cacggttgcg gatcgctccg tcctgtaccg aatcggccgg ggagtgtgcc
     1201 gttcgtaccg tacggtacac tctgccgcgt cccggggttc ttgcccggcc gccctaagcc
     1261 gctgtttttc cggaaattag cctcgccccg ccgccgttgg cacggttgtt gatcctatct
     1321 gtggatgacc gaaggaaccc ggaaggagaa ggaaggagaa ccgaccatgc ctcgcacagc
     1381 gctcacgctg gtccccctca tgatcctcgc ccttctggtt ttcggctgtt ccgacaaccc
     1441 cgccgacacc gccgccacca attccgccga tctgaatgac ttcttcggcg gttacactgc
     1501 gactccggaa ccgccggggt tcggagatcc ttcgctgctg gccggggcgg gcagcgaggt
     1561 gagttacgac gatccgatcc tggcttcccc ggcggccgac tccatcgtcg gcgatcccga
     1621 ggcggggttc ttctctctcc gcgtcgtgtg ggggcggctc cgctacgact cgacagtcac
     1681 gacgcccacc gattggaccg ggtcgctggc ggtcacgcgg ggcgtcgaga tcgtccgccg
     1741 cgtcatcgct ttcgagcccg cacaggacta cgttctcgaa cggaccgacc cgaaactcgt
     1801 cgcgtgggtg agccggacaa cggtgcatca cgacggcgtg gctatcgaca tctttgtccc
     1861 ccggccgcac gccgtggtcg acaccgtcga gattccgatc gtcgacacgc tcggcgacac
     1921 cacctggcag acggtcattg atactttccc gagcgagccg gttgaggtaa cgtttgcgac
     1981 cggtccctac acgcgcacct tcacgctcgg tgagctggtg gccctggaca cgatcgtcta
     2041 cctcgacgac tccaacgctg tcgcattcca cgctttccgg atggaccgcg tcccctgcgc
     2101 gcgcggtttc ctcgccggct tctggggcgt cgatgaagaa gggaacaacg aatttcgcgg
     2161 gatgtggatg tcccgctttg gctacctcga gggctggctg caggggacgt atggccgcaa
     2221 cgacgccggc gaaaacgtat ttgtcggcaa atgggtcgac gaccggggga acttcgaagg
     2281 gttcctccgc gggacctgga tgccgcatcc cgaccgccat
//
LOCUS       DAOOKS010000307         2319 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_57455_length_2319_cov_3.906802, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000307 DAOOKS010000000
VERSION     DAOOKS010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57455_length_2319_cov_3.906802"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            381..1082
                     /locus_tag="PK112_05655"
     CDS             381..1082
                     /locus_tag="PK112_05655"
                     /inference="COORDINATES: protein motif:HMM:NF040466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdjY domain-containing protein"
                     /protein_id="HPC11567.1"
                     /translation="MTHSLFPALVSLLAAVAAAGVRSETDVPSPPADTLVRYVSDHEI
                     RVGNVLMDISALTVSAPGWVNQKTGLVEYLAVTSEGKRHESVLVLDVQPLHLQTALLL
                     FGLDFGQNLAFQGDSVPPRGDSVDISVAWLGPEGDSVVVPASELLFDYHDSVVAPTTP
                     WVFTGSMIHLDRLQADLEGSIIATYSDPVAILNNPASARSDDTFYGVNEALVPPPGTP
                     VVLIITVPLRTGEDK"
     gene            1079..2257
                     /locus_tag="PK112_05660"
     CDS             1079..2257
                     /locus_tag="PK112_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="terpene cyclase/mutase family protein"
                     /protein_id="HPC11568.1"
                     /translation="MKTFAAVAILAAFPLLIAPPLPAQPAHPFDLSLRHEVERSVAAG
                     LQYLAGTQKDNGSWSYYVGITGLATTAFLQAPSAVKEQYWANIDRGLAFVLANVRPNG
                     GLYPDIEPQLHAYNTAICLMALVAADNPAYKDIIINARDYLLSLQADEDEGISPDSST
                     YGGIGYNRDERSDLSNMQFALEALRASEDFKPTAEYAGKVEYRGDAVQETAEVSNKEL
                     FWEKAILFLQRSQNYRKYNDYEWSSDDGGFIYYPGSSKAGGTTSYGGMTYAGMKSFIH
                     AGLRREDERVQKAYAWIRANYTVEENPELGRQGLFYFYSVMAKALSLFGDSLLVDSGG
                     VAHDWRGDLADKLISIQQSDGSWVNENGRWWENNPDLVTAYCVMALEKLLQPLAPKME
                     "
BASE COUNT          425 a          766 c          722 g          406 t
ORIGIN      
        1 cctgagccgg caccgcggcg gtgtcgcgag cggcctgggg agcgcccaca gccccaggtc
       61 cgatcaaagc cgctcccgct gtcacgatgc agagtgtggc cgcacgaaag tacatacagg
      121 gtgttttcgg cactccgaag tcgtcgatgt gagccgggcc tggggaaacc gcctgcgtct
      181 tgccgggcgc cccgccgcct gcccacaaga taccatcgtt tcgcgcgaag tcaaggcgcg
      241 gatcgtagta taattgaacg atttatcctt gacagcgcag cgttccgtgc gtagcttggg
      301 ccaagacaaa gagcagggcg aggacccttt cttcgagcga ctctgaccca gtgccgggcc
      361 gccgatccga acagaatgtt atgacacact ccctgttccc cgcgctcgtg agcttgctgg
      421 cggctgtggc tgccgcgggc gtgcggtcgg aaaccgatgt accctccccc cccgcggaca
      481 ccctcgtccg gtacgtgagt gaccatgaga ttcgcgtcgg gaatgtcctg atggacatct
      541 cggcgctgac ggtttcggcg cccggctggg tgaaccagaa aaccggtctc gtcgagtacc
      601 tggcggtcac atccgaaggg aagcgtcatg agtcggtgct ggttctcgat gtccagccgc
      661 tccatctcca gaccgctctg ctcctgttcg gtctcgattt cggccagaac ctcgccttcc
      721 agggggactc cgtccccccg cgcggggaca gcgtcgacat ttcggtcgcc tggctaggcc
      781 cggaggggga ttcggtggtg gtgccagcct ccgagctgct ctttgactat cacgactcgg
      841 tggtggcgcc taccactccg tgggtgttca ccggctccat gattcacctg gatcggctcc
      901 aggccgacct tgagggatcg atcatcgcga cctattccga tcccgtcgcc atcctcaaca
      961 acccggcctc ggcccggtcg gacgatacct tctacggcgt gaacgaagcg ctcgtcccgc
     1021 cccccggcac accggtggtc ctgattatca cagtgccgtt gcgcacggga gaagacaaat
     1081 gaagacgttc gcggccgtcg cgatcctggc ggcgtttcct ctcctgatcg ccccgcccct
     1141 gcctgcccag ccggcgcacc cgttcgacct ctcgctgcgg catgaggtcg agcgaagcgt
     1201 tgcggccggc ctgcagtacc tggcgggcac gcagaaagac aacggctcgt ggagctacta
     1261 cgtggggatc accgggctgg cgacgaccgc tttcctccag gcgccgtcgg ccgtgaagga
     1321 gcagtactgg gccaacattg accgcggcct ggcgtttgtg ctcgccaatg tccggcccaa
     1381 cggcggcctc taccccgata tcgaaccgca actgcacgcc tacaacacgg ccatctgcct
     1441 catggccctc gtggccgccg acaacccggc ctacaaagac atcatcatca atgcgcgcga
     1501 ttacctcctc agccttcagg ccgacgaaga cgaggggatc agtccggaca gctcgaccta
     1561 cggcggtatc ggctacaatc gcgacgagcg ctccgacctg tccaacatgc agttcgccct
     1621 cgaggcgctg cgggcatccg aggacttcaa accgaccgcc gagtacgccg gcaaggtgga
     1681 ataccggggg gacgcggtgc aggagacggc tgaagtctcc aacaaggagc ttttctggga
     1741 gaaggccatc ctgttcctcc agcgttcgca gaactaccgc aagtacaacg actacgagtg
     1801 gtcgagcgac gacggcggct tcatctacta cccgggatcg tcgaaagccg gcggcacgac
     1861 gtcgtacggc ggcatgacct atgccggcat gaagagtttc atccacgccg ggctgcgccg
     1921 ggaggatgag cgggtgcaga aagcgtatgc ctggattcgc gccaactaca cggtcgagga
     1981 aaatcccgag ctcggccgcc agggtctctt ctacttctac agcgtgatgg ccaaggcgct
     2041 ctcgctcttt ggcgacagcc tgctcgtcga cagcggcggc gtcgcccacg actggcgggg
     2101 cgatctcgcc gacaaactga tttcgataca gcagtccgac ggttcctggg tcaacgagaa
     2161 cgggcgctgg tgggaaaaca accccgacct ggtgacggcg tactgcgtga tggctctgga
     2221 gaaactgctg caaccgctgg cgccgaagat ggaatagaat gacaacgcgg tggcgccggc
     2281 accgccgcaa gagaccggca gtcgccggtc ccggcgggc
//
LOCUS       DAOOKS010000308         2318 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_57492_length_2318_cov_4.077773, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000308 DAOOKS010000000
VERSION     DAOOKS010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57492_length_2318_cov_4.077773"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2311
                     /gene="mfd"
                     /locus_tag="PK112_05665"
     CDS             <1..2311
                     /gene="mfd"
                     /locus_tag="PK112_05665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HPC11569.1"
                     /translation="PHPSRGSRLDLLAETAREFDALGMVHFVATDTESQAERLDELIG
                     ERLGPESRPVIEPADLKGGFVCPAGRFAVLTDHEIFGRHHRRVRRKKFREGVAISDYS
                     ALNRGDFVVHTDHGIAKYLGLETLVVDGRNRDCLALLYEDNDKLYVPIEEFNRVSKYA
                     GKDTAPQLTRLGGPQWDKLKVRTKKAIADMAADLLKLYARRRSAPGFSFGEDTTWLRQ
                     LETSFRYEETPDQLKAIADVKRDMAREQPMDRLIVGDVGYGKTEVAVRAAFKAIERGK
                     QVAVLVPTTILAQQHFQTFTERLAEFPMRVEMLSRFRTAKQQRAVVEDLAAGRVDLVI
                     GTHRLLSSDIFIPNIGLLIVDEEHRFGVRHKEKLRHLKAAVDTLAMSATPIPRTLHMS
                     LMSVRDMSIINTSPKDRLPIITEIVEFDPAIIATAILREIGRGGQVFFVHNRVQTIEA
                     MHHYLKKIVPQAEIAIAHGQMHERSLEGIMLAFLARRYNVLLCTSIIESGLDIPNANT
                     IIINRADRFGLAQLYQMRGRVGRSATRAYAYLMTPPVKQLQPEAIKRLRALEAHSDLG
                     AGFALAMRDLEIRGAGTLLGPRQSGYIEEIGFDMYNKLLEEAIAELKGEELVRPPDTK
                     LELDLELHLPDEYINDRQQKVDIYRRLADGRTLDDVERIRDEVTDRFGAQPQSAVNLF
                     DATAVKILAGAMKVEKVKMAAGRVSLFLEEGRQLTRRTVEALRQGADCPMEFSMIGRV
                     QIMLDLGRIGERDRLPYLRKVLSGVEPGG"
BASE COUNT          433 a          806 c          721 g          358 t
ORIGIN      
        1 gccgcacccc tcgcggggct cgcgccttga cctgctggcc gagacggcgc gcgagtttga
       61 cgcgctcggg atggtccact tcgtcgccac cgacaccgag agccaggccg agcggctcga
      121 tgagctgatc ggcgagcggc tcggcccgga gagccggccg gtgatcgagc cggccgacct
      181 caaaggaggg tttgtctgtc ccgccgggcg tttcgccgtg ctcaccgacc acgagatatt
      241 cggccgccac caccgccgcg tgcggcgcaa gaagttccgc gagggcgtcg cgatctccga
      301 ctactctgcg ctcaaccgcg gagatttcgt cgtccacacc gatcacggca tcgcgaaata
      361 cctcgggctg gagacgctcg tggtcgacgg ccgcaaccgc gactgcctcg ccctccttta
      421 cgaagacaac gacaagctct atgtcccgat cgaagagttc aatcgcgtct ccaaatacgc
      481 cggaaaagac accgcgccgc agctgacccg cctgggcggc ccccagtggg acaagctcaa
      541 ggtgcggacc aagaaggcga tcgccgacat ggccgccgat ctcctgaaac tgtacgcccg
      601 gcgccggagc gcgcccggtt tctccttcgg cgaggatacc acctggctgc gccagctgga
      661 gacctcgttc cggtatgagg agactcccga ccagctcaag gcgattgcag acgtcaagag
      721 agacatggcc cgcgaacagc cgatggaccg gttgattgtg ggcgatgtcg gctacggcaa
      781 gaccgaggtg gcggtgcggg cggcgttcaa ggcgatcgag cgcggcaagc aggtcgcggt
      841 gctggtgccg accaccattc tcgcccagca gcacttccag acgttcaccg aacggctcgc
      901 cgaattcccg atgcgcgtgg agatgctctc gcggttccgg acggccaagc agcagcgcgc
      961 ggtggtggag gatctcgccg cgggccgggt cgatctcgtg atcggtaccc atcggctgct
     1021 cagcagcgat atcttcatcc ccaacatcgg tctgctcatt gtcgacgaag agcaccgctt
     1081 cggcgtccgc cacaaggaga aactccggca cctgaaagcg gcggtcgaca cgctggcgat
     1141 gtcggcgacg ccgattccgc gcacgctcca catgtcgctc atgagcgtcc gcgacatgag
     1201 cattatcaac acctcgccca aggaccgcct ccccatcatc accgagatcg tcgaattcga
     1261 cccggcgatc atcgccaccg ccatcctgcg ggagatcggc cgcggcggcc aggtgttctt
     1321 cgtccacaac cgggtgcaaa caatcgaggc gatgcaccac tacctcaaga agattgtccc
     1381 gcaggccgaa atcgccatcg cccacggcca gatgcacgag cggtcgctcg aggggatcat
     1441 gctcgccttc cttgcccgac gctacaacgt gctcctgtgc acctcgatca tcgaatccgg
     1501 cctcgatatc ccgaacgcca acacgatcat catcaaccgc gccgaccggt tcgggctggc
     1561 ccagctctac cagatgcgcg ggcgggtcgg gcggagcgcc acccgcgcct acgcctacct
     1621 gatgaccccg ccggtcaagc agctccagcc ggaggcgatc aagcgcctgc gcgcgctcga
     1681 ggcccactcc gacctcggcg ccgggttcgc cctggccatg cgcgatctcg aaatccgcgg
     1741 cgccggaacc ctgctcgggc cgaggcaatc cgggtacatc gaggagatcg gcttcgacat
     1801 gtacaataag ctgctcgaag aggccatcgc cgagctcaaa ggcgaagaac tcgtccgtcc
     1861 ccccgacacc aagctcgaac tcgacctcga actccacctc cccgatgagt acatcaacga
     1921 ccgccagcag aaggtcgata tctatcgccg cctcgccgac ggccggacgc tggacgatgt
     1981 ggagcggatc cgcgacgagg tgaccgaccg gttcggcgcc cagccccagt cggcggtgaa
     2041 cctgttcgac gccaccgccg tgaagatcct cgccggcgcg atgaaggtcg agaaagtgaa
     2101 aatggccgcc ggccgcgtct ccctgttcct cgaggagggc cgacaactga cgcgccggac
     2161 ggttgaggcg ctccgccagg gggccgattg cccgatggag ttttccatga tcggacgggt
     2221 gcagatcatg ctcgatctcg gccgcatcgg cgagcgcgac cggctgccct atctgcgcaa
     2281 ggtactctcc ggcgtggagc cgggcgggtg agttgagc
//
LOCUS       DAOOKS010000309         2318 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_57508_length_2318_cov_3.038886, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000309 DAOOKS010000000
VERSION     DAOOKS010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57508_length_2318_cov_3.038886"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /locus_tag="PK112_05670"
     CDS             <1..990
                     /locus_tag="PK112_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11570.1"
                     /translation="LFYGLQSETTRDIFSNQFDFEGYRVYLARDNRESSFSLVGQYDR
                     EDYDKWVLVQKPGRDAQYYRQGDPFTRGFLRCAYGQGPNPCADSAFDPLLYTISRPYV
                     HPLYPDSVFYFTAHDYNQYAFGAGTSIRKVYPAQEPPVSLQAPAPEDTTEEGLFKYYL
                     YEMDIPDLLPSVQYYVVVTAFDFGSPETGLAPQESARTVYAKPAYPNNAWDEKPDSLT
                     NVYVYPNPYRHDAYYRVSGFEGRGEEDRSRDHVRKITFANLPHQCTIRIFTLDGDLVK
                     ELNHDIPSSDPNSSYHEWDMVSRNFQMIVSGLYYWVVETPDGRHQVGKLVILL"
     gene            complement(1064..>2318)
                     /locus_tag="PK112_05675"
     CDS             complement(1064..>2318)
                     /locus_tag="PK112_05675"
                     /inference="COORDINATES: protein motif:HMM:NF023428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HPC11571.1"
                     /translation="EFVGQESVVGPGKPLRKAIEEDRVSSIIFWGPPGSGKTTLAALI
                     ARATRGQFVPFSAMTSSIKDVKEVLTKAGNYYRMSGHRTYVFIDEIHRFNKAQQDAFL
                     PYVEKGDIVLIGATTENPSFEVNSALLSRMRVYALDRLSPEGIRGVARRALEDAGRGL
                     GEMRLTVDEEALDFLAAAADGDARRGLSLLEAAATYVGQGGAITAAVLRDIHQKGALV
                     YDKAGEEHYNLISALHKSIRGGDPDASLYWLARMLESGEDPLYIVRRLIRFATEDIGL
                     ADPYALTLALNARDTFHFLGSPEGELGIAMAVIYMACAPKSNAAYVAFDRAKADAAEK
                     GSLPVPLHIRNAPTRLMKDLGYGKGYQYAHDFTDGLTDQEHFPDELAGTRYYEPKGAG
                     REAKMREYLDKYRAFRARGDKPSKS"
BASE COUNT          388 a          802 c          712 g          416 t
ORIGIN      
        1 ctcttctacg gactccagtc agagaccacc cgggacatct tctccaacca attcgacttc
       61 gagggctacc gcgtctacct cgcccgcgac aatcgggaga gcagcttctc gctcgtcggc
      121 cagtacgacc gcgaggatta cgacaagtgg gtgctcgtgc agaaacccgg gcgcgacgcg
      181 cagtactacc gccaggggga tccgttcacc cgcggatttc tccgctgcgc ctacggccag
      241 gggcccaacc cgtgcgccga ttccgctttt gaccccctcc tctacaccat ctcccgcccg
      301 tacgtgcacc cgctctatcc cgactcggtg ttctacttca cggctcacga ctacaaccag
      361 tatgccttcg gggccgggac ctcgatccgc aaggtctatc cggcgcagga gccgcccgtg
      421 tcgctgcagg cgccggcgcc cgaggacacg accgaggaag gtctgttcaa gtactatctg
      481 tacgagatgg atatccccga cctgctcccg agcgtgcagt actacgtggt ggtgaccgca
      541 ttcgacttcg ggtcgcccga gaccggcctg gccccgcagg agtccgcgcg cacggtctac
      601 gcgaagccgg cttatcccaa caacgcctgg gacgagaagc ccgactcgct gaccaacgtg
      661 tacgtctacc ccaacccgta ccggcacgac gcctactatc gcgtctccgg tttcgaggga
      721 cggggcgagg aggaccgctc gcgtgatcat gttcgcaaga ttacgtttgc caatctgcct
      781 caccagtgca cgatccgcat tttcacgctc gacggcgacc tggtgaaaga actcaaccac
      841 gacattccgt ccagcgatcc gaactcctcc taccacgagt gggacatggt gagccggaac
      901 ttccagatga tcgtctccgg gttgtactac tgggtggtgg agacgccgga cgggcggcat
      961 caggtgggca agctcgtgat tctgctgtga ggggatggcg gccgcggcgg cccgaggcgg
     1021 cggcccggcc ggacggtccg cgcccggcgc gcctccggag gggctaggac ttcgagggtt
     1081 tgtcgccgcg ggcccgaaac gcccggtact tgtccaggta ctctcgcatc ttggcctccc
     1141 gtcccgcgcc tttcggctcg tagtagcgtg tccccgccag ttcgtcgggg aaatgctcct
     1201 ggtcggtcag cccgtcggtg aagtcatggg cgtactggta gcccttgccg tagccgaggt
     1261 ctttcatcag gcgggtcggg gcgttgcgga tgtgcagggg gacgggcagc gatccctttt
     1321 cggccgcatc ggccttggcc cggtcaaacg ccacataggc ggcgttggat ttgggcgcgc
     1381 aggccatgta gatcacagcc atggcaattc ccagttcccc ctccggcgaa ccgagaaaat
     1441 ggaaagtgtc gcgcgcattg agcgccagcg tcagggcata ggggtcggcc agtccgatat
     1501 cctcggtcgc gaaccgaatc agccgccgca cgatgtagag cggatcctcg cccgactcga
     1561 gcatgcgcgc cagccagtac agggaggcat ccgggtcgcc cccccggatc gacttgtgga
     1621 gcgcggaaat gaggttgtag tgctcctccc ccgccttgtc gtacaccagc gctcccttct
     1681 ggtggatgtc gcggagcaca gcggcggtga tggcgccgcc ctggccgaca taggtcgccg
     1741 ccgcctcgag cagcgacagc ccccgccgcg cgtcgccgtc ggcggccgcc gcgaggaaat
     1801 cgagcgcctc ctcatcgacc gtcaggcgca tctcccccag cccccgcccg gcgtcctcca
     1861 gcgcccggcg cgccactccg cggatccctt ccggcgacag ccggtcgagc gcgtagaccc
     1921 gcatccgcga caacagagcc gagttgacct cgaacgaggg attctcggtg gtcgccccga
     1981 tcagcacgat atcccccttc tcgacatacg gcaggaaggc atcctgctgg gctttattga
     2041 atcggtggat ttcgtcgatg aagacgtagg tgcggtgccc cgacatccgg tagtaattgc
     2101 ccgccttggt gagcacctcc ttgacatcct tgatcgacga ggtcatggcc gagaacggca
     2161 cgaactgtcc cctggtggcg cgggcgatca gcgccgccag cgtcgtcttg ccgcttcccg
     2221 gcggtcccca gaagatgatc gaactgaccc ggtcttcctc aatcgccttg cgcagcggtt
     2281 tccccggccc gactacgctc tcctgtccga cgaactcg
//
LOCUS       DAOOKS010000310         2318 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_57509_length_2318_cov_3.019001, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000310 DAOOKS010000000
VERSION     DAOOKS010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57509_length_2318_cov_3.019001"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="PK112_05680"
     CDS             <1..219
                     /locus_tag="PK112_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11572.1"
                     /translation="RGNADGDPEDRVTVSDLLHLIHYVFRGQAGLPCLEEANINGSPD
                     GIIDVADVNLLIAHLYREDQVFLPAPCQ"
     gene            439..1254
                     /locus_tag="PK112_05685"
     CDS             439..1254
                     /locus_tag="PK112_05685"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HPC11573.1"
                     /translation="MYQRYAPGTPDCLRPTRLRLVPTLVLGLLLTGGLTAEVRAQLKA
                     APAAAGVVLSWTAPGDDGNTGTAASYDLRYARVPITAANWNDWGVMTACLNEPAPLAA
                     GTVQSYTIDSLDPSTTYYFAIKAADEAGNWSALSNVVSYATPAEDVPPAVITDLRVAA
                     VGQTSVTLGWTAPGDDGTTGTAASYTVKFSTSPITNANWAAATTVPGVPAPHAAGTAE
                     SLTVTGLTPGVTYYFAIKTSDEVPNTSGLSNVVSGTPTGDQTAPAAITDLDAS"
     gene            complement(1319..>2318)
                     /locus_tag="PK112_05690"
     CDS             complement(1319..>2318)
                     /locus_tag="PK112_05690"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fatty acid--CoA ligase family protein"
                     /protein_id="HPC11574.1"
                     /translation="GVMLSHRNLSANTAGTVEYLRLSCRDRVQVILPFYYIYGNSLLL
                     THAAVGGCLVIDNRFTYPETVLDTMERERVTGFSGVPSNFMILLGNTTFAKRAFPDLR
                     YFTQAGGAMAPEVIRRLREAFPTKEIFIMYGQTEASPRVSWLPPERLGEKLGSVGIPV
                     PGVQVRLVDAAGNDVAAGEEGEIIVGGDSVMMGYWKQPAEQAEVLREGWLYTGDLARR
                     DDEGYLYIVGRRKEIIKSGGNRVSAKEVEETILELTGIAEAAVYGVPDAVLGEAVAAA
                     VVPGTGAGVSANDVIVHCKARLAGHKVPKHVQFLAALPKYQSGKVNKQALKKTAPA"
BASE COUNT          360 a          930 c          641 g          387 t
ORIGIN      
        1 cgcggcaatg ccgacggcga cccggaggac cgcgtcaccg tcagcgacct gctccacctc
       61 atccactacg tctttcgcgg ccaggccggc ctcccttgcc tcgaggaggc taacatcaac
      121 ggcagccccg acggcatcat cgacgtggcc gatgtcaatc tgctgatcgc gcacctctac
      181 cgcgaggacc aggtgttcct gccggcgccg tgccagtgac gggcgctccc tcgcggcgcc
      241 ggcctttctt ccgccggcgc ccccggagga gtctgtacgc gatctgaccc cttgtgctta
      301 ttttcaccag ccgccgccgc ccggagaccc cgactcggcc gctgcggccg cctaactgct
      361 tgtcccctaa cgcccgattt ctccccctcg ccggcactcg atttgcccct ctccccaacg
      421 tcagaatacg gaggtgttat gtaccaacgc tacgccccgg ggacaccgga ttgtctccgc
      481 cctacccgcc tgcgccttgt ccctaccctg gtccttggtc ttctgttgac cggcggcctg
      541 accgccgagg tccgtgccca gctcaaagcc gcgccggcgg ccgccggcgt cgtcctttcg
      601 tggacagcac cgggcgacga tggcaacacg gggacagccg cctcgtacga cctgcgctac
      661 gcccgcgtcc ccatcaccgc cgccaactgg aacgactggg gcgtcatgac cgcctgcctg
      721 aacgagcccg cgccgctggc cgccggcacc gtccagtcgt ataccatcga ttccctcgat
      781 ccgtcgacca cttactactt cgccatcaaa gcggccgacg aggccggcaa ctggtcggcc
      841 ttgtccaacg tcgtctccta tgcgaccccg gctgaggacg tgccccccgc ggtcatcacc
      901 gacctgcgcg tcgccgccgt cggccaaacg tccgtcaccc tcggatggac cgcgcccggc
      961 gatgacggga caaccggcac cgccgcgagc tacaccgtga aattctcgac ctccccgatc
     1021 accaacgcca actgggcggc ggcgaccacc gtccccggcg tccccgcccc ccacgccgcc
     1081 ggtaccgccg agagcctcac ggtcaccggc ctgaccccgg gcgtgaccta ctatttcgcg
     1141 atcaagacgt cggacgaagt ccccaacaca tccggtctgt ccaacgtcgt ctccgggacg
     1201 ccgaccgggg atcagaccgc ccccgcggcc atcaccgact tggacgccag ttgatgcgtg
     1261 tgaaacctgc cggcccgcgg ccgacagcct ccttttgtgt ggaagagaaa cggccccgct
     1321 acgccggggc cgttttcttg agggcctgct tgttgacctt ccccgactga tacttcggca
     1381 gggcggcgag aaactgcacg tgtttcggca ccttgtggcc ggccagccgc gccttgcagt
     1441 gcacgatcac gtcgttggcg ctcacgccgg cgccggtccc cggcaccacc gcggccgcca
     1501 ccgcctcgcc cagcacggcg tccggcaccc cgtacacggc ggcctcggcg atcccggtca
     1561 gttccagaat cgtctcctcg acttctttgg cgctcacccg gttcccgccg ctcttgatga
     1621 tctccttgcg ccgccccacg atgtacaggt atccctcgtc atcccgccgc gcaagatccc
     1681 ccgtgtacag ccacccctcg cgcagcacct ctgcctgctc ggccggctgc ttccagtaac
     1741 ccatcatcac cgagtcccct ccgacaatga tctccccttc ctcgcccgcg gcgacgtcgt
     1801 tccccgcggc gtccactagc ctcacctgca ctcccggcac cggtatgccg accgatccca
     1861 gcttctcccc cagccgctcc ggcggcagcc acgacacccg cggggaggcc tccgtctgcc
     1921 cgtacataat gaaaatctct ttggtcggaa acgcctcgcg cagccgccgg atcacttccg
     1981 gcgccatggc cccgcccgcc tgcgtgaaat accgaaggtc ggggaacgcc cgcttggcga
     2041 aggtcgtgtt ccccaggaga atcatgaaat tcgagggcac tcccgagaac cccgtcaccc
     2101 gctcccgctc catcgtgtcc agcaccgtct ccgggtacgt gaaccggttg tcgatgacca
     2161 ggcagccccc caccgccgcg tgcgtgagca ggagcgaatt gccgtagatg tagtagaacg
     2221 ggaggatcac ctgcacccgg tcgcggcagc tgagccgaag atactccacc gtcccggccg
     2281 tgttcgccga gaggttccga tgcgacagca tcaccccc
//
LOCUS       DAOOKS010000311         2311 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_57772_length_2311_cov_3.233156, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000311 DAOOKS010000000
VERSION     DAOOKS010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57772_length_2311_cov_3.233156"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1382)
                     /locus_tag="PK112_05695"
     CDS             complement(<1..1382)
                     /locus_tag="PK112_05695"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HPC11575.1"
                     /translation="MARAYPGIFLLIALAGGILAVDFLRASAPAFLLAALGGLVAAAA
                     ALVFRRPSAAAVFFGLVLAGLSAAHFARDTYDLGSRSLGRYADSRTRYRLVGRVDDWP
                     ELRPDRIELTVRLDSLGPDRMAAVEGRLLLRIADTGAAVQFGDRVELSTRLYPAAERG
                     EGAASRRSLLVKGVGALAYVRASQDVRRLPRPAVGLIPVVDTVRAAIGQSLRRTLSPR
                     AAALAGGFLIGDTRDIPPDLYRAFRDSGTLHVLAVSGSNVAVVVGFVLVLLRPFRTGR
                     VARTLILLVVVVFFALLCRGEPSVVRASVMAALILLARLAGRRIDLNNLVALAAAAIL
                     LCAPAQLFSVGFQLSFATAWGLVFVTPRVYRLLEGRALGPPARWLSLAVIVSLTAQLF
                     SAPLVACYFHRVPLLGPAANLVIVPLVSAAVVGVLATLTLDFVWPPLGALAGALVDRL
                     MDLILGALGL"
     gene            complement(1382..>2311)
                     /locus_tag="PK112_05700"
     CDS             complement(1382..>2311)
                     /locus_tag="PK112_05700"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HPC11576.1"
                     /translation="IGYRFRFTISLFDAGLRRVMRLLTPMIIGLSAGRINILVSTLIA
                     SFLAEGAISYLNYSYRLMHFPLGVFAVAIGTVALPQVAELVARRQHDRVGDTFLNAVG
                     LNSFVVIPSAVFLALMGRPLVDLVYQWGAFSSLDSEQTALALLHYSYGLVGFAVVRVV
                     VPFYYAYEDSRLPVKISILAVVVNLVLYAPLVWLLGFAGLAAATSLAALLNAGLLIWF
                     LPRKGVAVSFGRLAFHTARITVIALVAFYVARVIPYDAVIALDDGLPQRTLAVILPAL
                     VGAVLYVAFSLALRTREITLLRNRLVRRRRAGN"
BASE COUNT          522 a          712 c          851 g          226 t
ORIGIN      
        1 aacaggccca gcgccccgag gatgagatcc atcaggcggt ccacgagggc gccggcgaga
       61 gcgccgagcg gcggccagac gaaatcgagc gtgagagtcg ccagcacccc cacgaccgcc
      121 gccgacacga gcgggacaat gaccaggttg gccgccggcc caagaagcgg cacacggtga
      181 aaatagcagg cgacgagggg agcggaaaag agctgcgccg tcagcgacac gatgaccgcc
      241 agcgacaacc accgggccgg cgggccaagc gcccggcctt cgagaagccg gtagacccgg
      301 ggcgtgacga agaccagccc ccaggcggtc gcgaacgaca attgaaaacc gacgctgaac
      361 aactgggcgg gggcgcacag gaggatggcg gcggcggcca gggcgaccag gttgttcaga
      421 tcgatgcgcc gccccgcgag gcgggccagc agaatgagag cggccatgac ggaggcgcgt
      481 acaaccgagg gttcgccgcg gcagaggagc gcgaagaaca caaccaccac aagcagaatg
      541 agggtgcgcg cgacccggcc ggttcggaag ggccgcagga gcaccaggac gaaacccacg
      601 accacggcca cgttcgaacc ggacacggcg agcacgtgta aagtgcctga gtcgcggaaa
      661 gcgcgataca ggtcgggggg gatgtcgcgg gtgtcgccga tgagaaaacc cccggccagc
      721 gccgcggcgc gggggctcag tgtccggcgg agcgactgcc cgatggcggc gcgcacagtg
      781 tcgaccacgg ggatcagccc aaccgccggg cgcggcaggc ggcgcacgtc ctgagaggca
      841 cggacataag ccagcgctcc cacgcccttt accagcagcg atcggcggga ggcggccccc
      901 tctcccctct cggccgccgg atacaggcgg gtggagagct cgacccggtc gccgaactgc
      961 acggccgcgc cggtatcggc gattctcagg aggagacggc cctcgaccgc ggccatgcgg
     1021 tcgggtccga gggaatcgag gcggacggtc agctcgatgc ggtcggggcg gagttccggc
     1081 cagtcatcga cgcggccaac cagccgatag cgggtgcggc tatcggcgta gcggccgagg
     1141 ctgcgggaac cgagatcata ggtgtcgcgg gcgaaatgtg cggccgagag gccggccaag
     1201 acgaggccga aaaacacggc ggcagccgac ggtcgccgga aaaccagggc ggcggcagcc
     1261 gccaccaacc cgcccagcgc ggccagcaaa aaggccgggg cggaggcgcg gaggaaatca
     1321 acggcgagaa tgccgccggc aagagcaatg agaaggaata tccccggata cgcgcgcgcc
     1381 atcaattccc cgcccgacgt cttcgcacta acctgtttct caacaaggta atctcgcgag
     1441 tacgcagggc caaggaaaaa gcaacgtaga gcacggcgcc caccagcgcc ggaagaatca
     1501 ccgccagggt ccgttgcggg agaccgtcat cgagggcgat gacggcgtca taagggatga
     1561 cgcgcgcaac atagaacgcc acgagcgcga tcacggtgat ccgcgccgtg tgaaaggcca
     1621 gacggccgaa cgaaacggcg actcccttgc gcggcagaaa ccagatcagc aggccggcgt
     1681 tgagcagggc cgccagagag gtggccgccg ccagtccggc aaacccgagc agccacacca
     1741 gcggagcgta gagaacgagg ttgacgacga cggcgaggat cgagattttc accggaagcc
     1801 gggagtcctc ataggcatag tagaacggca cgaccacgcg gacgacggcg aagccgacca
     1861 ggccgtagga gtagtgcagc agggcgagcg ccgtctgttc gctgtcgagg ctggagaagg
     1921 ccccccactg gtagacgagg tcgacgaggg gacggcccat gagggcgagg aagacggcgg
     1981 aggggataac cacgaaggag ttcaggccga cggcgttcag gaaggtatcg ccgacccggt
     2041 cgtgctggcg gcgggccacc agttcggcca cctgcggcag ggcgacggta ccgatggcga
     2101 cggcgaacac gcccaggggg aaatgcatga ggcggtagga gtagttgagg taggagatcg
     2161 ccccttccgc gaggaatgac gcgatgagcg tggagacgag aatgttgatg cggccggcgg
     2221 acagcccgat aatcatgggc gtcaacagcc gcatcacccg ccgcagaccg gcatcgaaca
     2281 gcgagatggt gaagcggaag cggtagccga t
//
LOCUS       DAOOKS010000312         2309 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_57841_length_2309_cov_3.459184, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000312 DAOOKS010000000
VERSION     DAOOKS010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57841_length_2309_cov_3.459184"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="PK112_05705"
     CDS             <1..718
                     /locus_tag="PK112_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003123128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N(G),N(G)-dimethylarginine
                     dimethylaminohydrolase"
                     /protein_id="HPC11577.1"
                     /translation="DGITSAGLGAPVHRLAVAQHRAYVEALRACGLAVTCLPADDEFP
                     DSTFVEDTALLTPHCAIIMRPGAPARRGEAAAIEPVVAGLFDAVERVEPPGTADAGDI
                     MMVGDHYFIGLSQRTNREGAAQIIALLEAYGMTGSPVPLDRVLHLKSGVVYLENNTML
                     AAGEFKERPEFRAYDLIPVDDDERYAANALWLNGTVLVAAGYPKTERAVRAAGYETIV
                     LEMSEFRKLDGGLSCLSLRF"
     gene            857..1981
                     /locus_tag="PK112_05710"
     CDS             857..1981
                     /locus_tag="PK112_05710"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HPC11578.1"
                     /translation="MTDTLGRKNLDEELRARSHALAERMKELECLHSVSDLFENRHIE
                     LSEIFQRIVELLPGAWQYPEIACARITMKSRQYQSHNYRETPWRQQADITAGGERVGV
                     IEVGYLEVLPGGTPPQFLPEEADLLETVAHRLGEAYSLKEAQRQLSTYQQHLRSLAME
                     LTLAEERERRQLALHLHDNIGQGLAVAKLKLETLRHLLPEGHGERIDDILALIQQIIA
                     DTRTITADISPPILYELRFDQALVWLGDHVRKQSGLQVEVHWPEEDVALSEGVRVLLF
                     RSIQELLANVVKHAGAAAVRIEVERQGNEAHVCVADDGVGFDLEHQGRYPSANGGFGL
                     FSIRERLTHLGGRMTVDPKVGGGTRVHLWVPVTDSPAAPR"
     gene            1995..>2309
                     /locus_tag="PK112_05715"
     CDS             1995..>2309
                     /locus_tag="PK112_05715"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPC11579.1"
                     /translation="MRILIADNHKLFCEGLRLLLEKQPHMEIVGEANNGRMAVRLCHE
                     LTPDLVVMDVGMPELNGIEATRQIRAEMPEVKVIAVSMHDDRQYVAGMLSAGASGYVL
                     KDG"
BASE COUNT          414 a          727 c          821 g          347 t
ORIGIN      
        1 tgacggcatt acctccgccg gcctcggcgc gccggtccac cggctcgcgg tcgcgcagca
       61 ccgcgcctac gtcgaggcgc tgcgcgcctg cgggctggcg gtcacctgct tgcccgcgga
      121 cgacgagttc ccggactcga cctttgtcga ggacacggcg ctgctgacgc cgcactgcgc
      181 catcatcatg cggccgggcg cccccgcgcg gcggggcgag gcggccgcca tagaaccggt
      241 ggtggccgga ttgttcgacg ccgtggagcg ggtcgagccg ccgggaacag ccgacgcggg
      301 ggatatcatg atggtcggag accactactt catcggcctg tcgcaacgca cgaaccggga
      361 gggggcggcc cagattatcg cgctgctcga ggcgtacgga atgaccggct ccccggtgcc
      421 gctcgaccgg gttctccatc tgaaatcggg cgttgtctac ctggagaaca acaccatgct
      481 ggcggccggg gaattcaaag agcggcccga gttccgggcg tacgatctga ttccggttga
      541 cgacgacgag cgctatgcgg ccaacgcgct gtggctgaac gggacggtgc tggtcgcggc
      601 cggctatccg aaaaccgagc gggccgtacg cgcggcgggg tacgaaacga tcgtgctcga
      661 aatgtcggag ttccgcaagc tcgacggcgg gttgagttgc ctgtcgttgc ggttctgagt
      721 tcgcgcgtct aaaggcgccg gcgagggcga cggtcctccg caggcggcga aaaaggcatt
      781 gacttcgcgc ggctgcagtg ctctattggt tagtgagagg cccgcggcca cgcgaggagc
      841 gggcaaggag ttgacggtga ccgacacttt gggccggaag aacctcgacg aggaactgcg
      901 ggcgcgctcg cacgctctcg ccgagcgcat gaaagagctc gaatgtctgc actcggtgtc
      961 ggatctgttc gagaaccggc acatcgagct gagcgagatt ttccagagaa tcgtggaact
     1021 cctccccggg gcctggcagt acccggaaat cgcgtgtgcg cggatcacaa tgaaaagccg
     1081 ccagtaccag agccacaact acagggagac gccctggcgg cagcaggcgg acatcaccgc
     1141 cggcggcgag cgcgtcgggg tcatcgaggt ggggtatctc gaggtcctgc ccgggggaac
     1201 cccgccccag ttccttcccg aggaagcgga ccttctggag acggtcgccc accggctggg
     1261 ggaggcctac tcgctcaaag aggcccagcg ccagctctcc acctaccagc agcacctgcg
     1321 gtcactggcc atggagttga cgctggccga ggagcgggaa cggcggcagc ttgcgctgca
     1381 cctgcacgac aacatcgggc aggggctcgc cgtggcgaaa ctgaaactcg agaccctccg
     1441 ccacctgctg ccggaggggc atggggagcg catcgacgat atcctcgcgc tcatccagca
     1501 gatcatcgcc gacacccgca ccatcaccgc cgacatcagc ccgcccattc tgtacgagct
     1561 ccgcttcgac caggcgctgg tctggctggg agaccacgtg agaaaacaat cgggactgca
     1621 ggtcgaggtg cactggccgg aggaggacgt ggcgctgagc gagggcgtgc gcgtgctgct
     1681 gttccgctcg atccaggagc tgctggccaa cgtggtgaag cacgccgggg cggcggccgt
     1741 gcggatcgag gtcgagcggc aggggaacga ggcgcatgtg tgcgtggccg acgacggcgt
     1801 cgggttcgac ctcgagcacc agggccggta cccctcggcc aacggcggct tcgggttgtt
     1861 cagcatccgg gagcgcctca cgcacctcgg ggggcggatg acggtcgatc cgaaagtggg
     1921 cggcgggaca agggtccacc tgtgggtgcc ggtcaccgac agcccggccg ccccgcgatg
     1981 aacaggagcc gaacatgcga atattgatcg ccgacaacca caagctcttc tgcgagggtc
     2041 ttcgcctgct gctggaaaag cagccgcaca tggagatcgt cggcgaggcc aataacggcc
     2101 ggatggcggt gcggctgtgc catgaactga cgccggacct ggtggtcatg gacgtcggca
     2161 tgcccgagct caacggcatc gaggcgaccc ggcagatccg ggcggaaatg ccggaggtca
     2221 aagtgatcgc ggtgtcgatg catgacgacc ggcaatatgt cgccgggatg ctctcggcgg
     2281 gcgcctccgg ctacgtcctc aaagacggc
//
LOCUS       DAOOKS010000313         2298 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_58258_length_2298_cov_3.407936, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000313 DAOOKS010000000
VERSION     DAOOKS010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58258_length_2298_cov_3.407936"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="PK112_05720"
     CDS             complement(<1..483)
                     /locus_tag="PK112_05720"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPC11580.1"
                     /translation="MIESEELKESVKTRLEESGIIGTMAERICHSLLSEKPPGLSPDE
                     YFHLVSISLLELLRARLLASCEPDRLVGPLDRGQLLDYDRILQGLLLTLERDLLRSHL
                     HMIRALGGAIAERDTGTNDHSYRVTIFAVRIAEAMGLPRQTIRSLIKGAFLHDIGKIG
                     I"
     gene            complement(631..>2298)
                     /locus_tag="PK112_05725"
     CDS             complement(631..>2298)
                     /locus_tag="PK112_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11581.1"
                     /translation="CRAGHYPNDCTVRSQVMQKSPATAVTDSPPLAPAGPGRDRAWVR
                     LPLALLLVIAAVHLALNLGINDGHLTYTLDDAYIHMALAEGIAHGHFGINPGEVCAPS
                     SSILWPFLLAPFMLLSFGAVVPFVVNLACAAATVLLMVRTMAHGFSAAGLANPVKYAA
                     WVAAALVPVTNLIGLIFSGMEHSLQLLLAAAVVHGLVCALSCQRVPRWLPAALIAAPL
                     VRYEMFALTLPAAVCLYLSGHRRVALVSALTALGLAAGYSGYLAALGLGALPTSVAMR
                     SDVVCSGWSIAAITDHFRGNLLSGEGTLLAVVFIPLAAVALDRSRRREHRLLAAAGAA
                     GIVLHLSVGRLGGWFHARYDMYILLSTLLLTVFLASAPLGRFLQRAGGVRAAVLAVAA
                     VALVAFPQLHRLSDLQIAASNIYQQQYQMHRFITEYYHGPVAANDIGWVAFQNDETVV
                     DLVGLADSRNVGRRKEASSGQWMTEAAAAGGARLAIIYESWFAHIPDSWIPVADLFLG
                     HRRTSAADRKVTFYALDRAAADSARILLTRFAPTLPKGVRLDLRPSE"
BASE COUNT          456 a          680 c          849 g          313 t
ORIGIN      
        1 aatgccgatc ttgccgatgt cgtgaaggaa agcccccttg atgagggagc ggatcgtctg
       61 gcggggcaaa cccatcgcct cggcgatccg gacggcgaag atggtgacgc ggtagctgtg
      121 gtcgttggtg ccggtgtcgc gctcggcgat ggcgccgccg agcgcccgga tcatgtggag
      181 gtggctgcgc agaaggtcgc gttcgagggt cagcaggagt ccctggagaa tgcggtcgta
      241 gtcaaggagc tggccgcggt cgagcggacc gaccagccga tccggctcgc acgaggcgag
      301 cagccgggcg cgcaggagct caagcaggga gatcgagacg aggtggaagt actcatcggg
      361 agaaagtccc ggaggcttct cgctcaggag agagtggcag atcctctcgg ccatagttcc
      421 gattattccc gattcctcga gccgggtttt caccgactcc ttcagttctt cggattcaat
      481 catacaaccc tccacgagaa ccaggggtca ggaccgacag agccgaggtg agagccgacg
      541 atgcgacctg tacaatctat ataatagcgc cgggggacga aaagttcggg cggtcggggc
      601 cccggcggcc cccgacacgg tcgtgatgag ctactccgac gggcggaggt cgagccggac
      661 acctttggga agagtgggtg cgaagcgggt caggaggatg cgagcggagt cggcggcggc
      721 gcgatcgagc gcatagaaag tcacttttct gtcggccgcg ctcgtgcggc ggtgaccgag
      781 gaagagatcg gccaccggaa tccaggaatc cggaatgtgc gcgaaccagg attcgtagat
      841 gatcgccaga cgggcgcctc ccgcagccgc cgcttcggtc atccactgac cgctggaggc
      901 ctccttccgg cgccccacgt tgcggctgtc cgccaggccg accaggtcga ccacagtctc
      961 gtcattctgg aaagccaccc agccgatatc gttggcggcc acggggccat ggtagtattc
     1021 ggtgatgaag cggtgcatct ggtactgctg ctggtaaatg ttgctcgcgg cgatttgcag
     1081 gtcgctgagg cggtgaagtt gcggaaaagc gacgagggcg acggctgcga cggcgaggac
     1141 cgccgctcgc acccccccgg cccgctgcag gaaccgtccc aggggagccg aggccaggaa
     1201 cacggtcagt aggagcgtac tcagcaggat gtacatgtcg tagcgggcgt gaaaccagcc
     1261 ccccagccgt ccgaccgaca ggtgcaagac aatgccggcg gccccggcgg cggcgagcag
     1321 gcggtgttct cggcggcggg agcggtccag cgcgacggcg gccaagggga tgaagacgac
     1381 agccaggagg gtcccctccc cactcagcag gttgccgcgg aaatggtccg tgatggcggc
     1441 tatcgaccag ccggagcaaa cgacgtcgga gcgcatcgcc accgaggtcg gcagcgcccc
     1501 gagcccgagg gcggccaggt acccggagta cccggcggcc agcccgaggg cggtcaaagc
     1561 ggagacgagc gccacccggc gatgcccgct gaggtagagg cacacggcgg ccggcagagt
     1621 caacgcgaac atttcgtacc ggacaagcgg ggcggcgatc agggcggccg gcagccaccg
     1681 gggaacgcgc tgacacgaca gagcgcacac cagaccatgc acgaccgcgg cggcgagcag
     1741 caactgcagg gagtgctcca tgccggagaa gatgaggccg atgaggttgg tcaccggaac
     1801 gagcgccgcg gcgacccagg cggcatattt gaccgggttc gcaagaccgg cggcgctgaa
     1861 accatgcgcc atcgtccgga ccatgagcag gacggtggcg gcggcgcacg cgagattgac
     1921 gacgaagggg acgacggccc cgaaggaaag gagcatgaag ggggccagca ggaagggcca
     1981 gaggatgctc gacgacggcg cgcagacttc gccgggatta atgccgaaat ggccgtgggc
     2041 gatgccctcg gcgagcgcca tgtggatgta cgcatcatca agcgtgtacg tcagatggcc
     2101 gtcgttaatg cccagattga gagcaaggtg gacagcggcg atgaccagga ggagggccag
     2161 cggcagacgg acccaggccc ggtcgcggcc ggggccggcg ggcgccagcg ggggcgaatc
     2221 ggtcacggcg gtcgcagggg atttctgcat cacctggctc ctcacagtgc agtcattggg
     2281 gtagtggcct gcgcggca
//
LOCUS       DAOOKS010000314         2297 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_58303_length_2297_cov_3.063336, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000314 DAOOKS010000000
VERSION     DAOOKS010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58303_length_2297_cov_3.063336"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..830
                     /locus_tag="PK112_05730"
     CDS             81..830
                     /locus_tag="PK112_05730"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPC11582.1"
                     /translation="MTAQEPWYEREDFWEWTAPFMFGEERFERAASSVGELTAFLRLA
                     SPAPVLDLCAGPGRFAVPLAAAGYSVTAVDRSRAFLATLTARARGRSLDIETVCCDMR
                     EFRREGAFEAVLNMYTSFGYFEDPEDDRRVLENVFSSLRPGGRLVMELHGRESLIRAF
                     RRRDWEEAHGITIIEERSFVDHFTRLHNRWIVLRGSERREFEFDLRIYGASELCALLR
                     SVGFASAEAYADFQGAPYDIAAGRLVALAAK"
     gene            complement(823..1728)
                     /locus_tag="PK112_05735"
     CDS             complement(823..1728)
                     /locus_tag="PK112_05735"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPC11583.1"
                     /translation="MLAYGGIPARLLRREDPTEPVFEQFRPDVFITLAEPAAQVGLDR
                     DYIGRYRRAHGLRVGHLTSFRHEYPPCDFLIALGCPADLPRADGAETPLLDMPPAINP
                     LRHYMRPRAEVWDYAFVGDNTPQTVERVKACLLPVLRLYSGVLAGRGWDIGLGELHQA
                     EEAVLFNFARVCPNLPLPGPSWESFAANLRALAIPACGGFALTDNTAFGGAFFAPDET
                     AAADSPGRFVEMFDYFRNHPDQRLPIIRAGMERVYREHTLFHRMDLLLAFLGCRGGSL
                     RRERQPVPPWEESATRPVTWADRLT"
BASE COUNT          380 a          740 c          853 g          324 t
ORIGIN      
        1 aacctgtcct gggacatcac cgttttggat aagatgttgg ggcgcccgaa cggccacggg
       61 aacagcccgg gaggaaagct atgaccgcac aagaaccctg gtacgagcgc gaggatttct
      121 gggagtggac ggcgccgttc atgttcggcg aggagcgttt tgagcgcgcc gcgtcatcgg
      181 tcggcgagct gacggcgttt ttgcggctgg cgtcgccggc cccggtgttg gacctgtgcg
      241 ccgggccggg gcggtttgcc gttccgctgg cggcggcggg ctattccgtc accgccgtcg
      301 accgcagccg ggcgttcctc gccaccctca ccgcccgcgc gcgcgggcgc agtctcgata
      361 tcgagaccgt ctgctgtgac atgcgcgagt tccggcgcga gggagcgttc gaggcagtcc
      421 tcaatatgta cacctcgttc ggatatttcg aggaccctga ggacgaccgg agggtgctgg
      481 agaacgtctt cagctcgctg cggcccggcg ggcgtctcgt tatggaactc cacgggcggg
      541 aaagcttgat ccgggcgttc cggcggcgcg actgggagga ggcgcacggg atcaccataa
      601 tcgaggagcg gtcgttcgtg gatcacttca cgcgcctgca caaccgctgg atcgtcctgc
      661 gggggagcga gcggcgggaa tttgaattcg atctgcggat ctacggagcc tccgagctgt
      721 gcgccctcct gcgctcggtg ggattcgcgt cggccgaggc gtacgccgac ttccagggcg
      781 cgccgtacga catcgcggcc ggacgactcg ttgccctcgc cgctaagtga ggcggtcggc
      841 ccaggtaacg ggccgggtgg ccgactcctc ccacggcggc accggctgcc gctcgcggcg
      901 caaagatccg ccgcggcatc cgaggaacgc cagcagcagg tccatccggt ggaagagggt
      961 gtgttcccga tagacccgct ccatgccggc gcggatgatc gggaggcgtt ggtccggatg
     1021 gttccggaag taatcgaaca tttccacaaa tcggcccgga gaatcggccg ccgcggtctc
     1081 gtcgggggcg aagaacgcgc cgccgaaagc ggtgttgtcg gtcagggcaa accccccgca
     1141 ggcgggaatc gcgagggcgc gcaggttcgc ggcgaaggac tcccacgagg ggcccgggag
     1201 ggggagattc gggcagacgc gcgcgaagtt gaacagaacc gcctcctccg cctggtggag
     1261 ctcgcccaga ccgatgtccc agccccgccc ggccagaact cccgaataca ggcggaggac
     1321 cggcaggagg caggctttca cgcgctcgac cgtctggggc gtattgtcgc ccacgaaggc
     1381 gtagtcccag acctcggcgc gcggcctcat atagtggcgc aacgggttga tcgccggcgg
     1441 catgtcaaga agcggcgtct cggctccgtc ggcgcgcgga aggtcggccg ggcaaccgag
     1501 ggcaatcagg aaatcgcagg ggggatattc gtgccggaac gacgtgaggt gcccgacgcg
     1561 aagcccgtgg gcgcggcgat atcgcccaat gtagtcgcgg tcgagaccga cctgcgcagc
     1621 cggttcggcg agcgtgatga agacatcggg acggaactgc tcgaagaccg gttcggtcgg
     1681 gtcctcccgc cgcaggaggc gggcgggaat cccgccgtag gcgagcacct cggcccagtt
     1741 ttcgatgagg gagcgcagcg cgccgggcgg cggcagatgc aggagaatgc ggtgtcggca
     1801 tgcacccagc ggttgcgacc ggccgcgaga gcttcctccc gcagccggag attctcccgc
     1861 aggaggggcg cggcggcgca gtccggggca tagcgcgaca gccaatggag agtgcggagc
     1921 aggtcgcgtt tgcggcggag atccggggcc aggcgcgcaa ggacccgccc ccacaggtcg
     1981 cagaggccgc ggtgggctga gcccagcatg gggtcggcgg ccagcgcccg ctgcagcagc
     2041 gcttccgcct ccgcttccag ccccgccgcc gccgcgacct cgccggccac cgcgagggcg
     2101 tcgggatcgt gcggcgcgag tctcatcacc gcccggacgc actcgagagc gccgggagca
     2161 tcgccggcct gcagccggag gcgcgcagcc gccgccaact gcggcacgga gaacagacag
     2221 ggcagagcag cccgacagga agaccgagcg gccgggccgg gccggagtgg tcggaacaac
     2281 ataaccccca ttttggc
//
LOCUS       DAOOKS010000315         2297 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_58313_length_2297_cov_1.970562, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000315 DAOOKS010000000
VERSION     DAOOKS010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58313_length_2297_cov_1.970562"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="PK112_05740"
     CDS             <1..296
                     /locus_tag="PK112_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11584.1"
                     /translation="GSFIGDHTKFGIGTLLTTGIAVGVCCNIFGGTLVADKEVASFRW
                     GSGGAWSPHRFDKALETARRTTERRHVSLSDREAALLQAIADETLGDAGVLPL"
     gene            363..1409
                     /gene="galT"
                     /locus_tag="PK112_05745"
     CDS             363..1409
                     /gene="galT"
                     /locus_tag="PK112_05745"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HPC11585.1"
                     /translation="MPELRKDPVIGRWVIISTERGKRPSSFQSVSKRGPARLCPFCPG
                     AESNTPREILAYRDPGTEPNKPGWSLRIFPNKYPALVIEGNLTREPHGVYDKMQGIGA
                     HEVIVETADHARDMDGMSEKELRDVFWAYRERMIDLERDRRFRYILVFKNHGQAAGAS
                     LEHSHSQLIATPIVPKKVQEEIEGSRRYYEFKERCVFCDIVRQEIMDQERIVTDFDSF
                     ITMQPFAARFPFETWLLPKTHQSSYLEMSDSDFWILARTFKDILTRLKVALNDPPFNF
                     VLHTRPMSPEHHTYYHWHFEIIPKLTRVAGFEWGSGFYINPTTPEQAAAYLRQIDVTA
                     PPAAAPSEISEKEV"
     gene            1412..>2297
                     /locus_tag="PK112_05750"
     CDS             1412..>2297
                     /locus_tag="PK112_05750"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HPC11586.1"
                     /translation="MKVCLVGPEMVPFAKTGGLGDVVGALPKALGRLGHDVTVFMPLY
                     RELDFGVHRVHAVDLAVSVPVAGSVQTVSLRAVRQKKHNVEIYFIGNRQYFDRDGFYV
                     DPATGKDHADNDERFIFFNRAVLEVLKALNLRPHVINVNDWQTALIPVYLRTLGAADP
                     FFAGVKTVLTIHNLAYQGSFPRERFARLELPGALFAPAAGPFEFYGQVNFLKAGITFA
                     DKITTVSKQYAREIQTAEFGAGLEGVLAGRAADLVGIQNGVDYTVWSPSRDKKIHYTY
                     HLANLSGKRMNKVELLNRA"
BASE COUNT          439 a          770 c          706 g          382 t
ORIGIN      
        1 tggggtcgtt catcggcgac cacacgaaat tcgggatcgg gacgcttttg acgacgggaa
       61 ttgcagtcgg agtgtgctgc aatatcttcg gcgggaccct ggttgccgat aaagaggtag
      121 cgtcgttccg ctggggatcc ggcggggcgt ggtcgcccca ccggttcgac aaagcgctgg
      181 agacggcgcg gcggacgacg gagcggcgcc acgtcagcct ctccgaccgg gaggcggcgc
      241 tgctgcaggc cattgccgac gagacgctgg gcgatgccgg cgtgctgccg ctgtgaggac
      301 gagacggccg aacggacaga ggggccgaag gtaacggaag accacaaagg gagtgatgcc
      361 gtatgcccga attgcgtaaa gacccggtga tcggtcgctg ggtgatcatt tcgaccgagc
      421 gggggaaacg cccgagttcg ttccagtcgg tctccaaacg gggaccggcg cggctgtgcc
      481 cgttctgccc cggggcggag agcaacaccc cgcgtgagat tcttgcctac cgcgatccgg
      541 gcaccgagcc gaacaagccc ggctggtcgc tccgcatttt ccccaacaag taccccgccc
      601 tcgtcatcga gggaaacctg acccgcgagc cgcacggggt ctacgacaag atgcagggga
      661 tcggcgccca cgaggtgatc gtcgagacgg ccgaccacgc ccgcgacatg gacgggatga
      721 gcgagaagga actccgcgac gtgttctggg cctaccggga gcgcatgatc gacctcgagc
      781 gcgaccgccg tttccgctac atcctcgtgt ttaagaacca cggccaggcc gccggcgcct
      841 cgctcgagca ctcccacagc cagctcatcg ccaccccgat tgtccccaag aaagtccagg
      901 aggagatcga ggggagccgc cgctactacg aattcaagga gcgctgcgtg ttctgcgaca
      961 tcgtccgcca ggaaatcatg gaccaggaac gcatcgtgac cgacttcgac tcgttcatca
     1021 cgatgcagcc gttcgcggcc cgcttcccct ttgaaacctg gctgctgccg aagacgcacc
     1081 agtcgagtta cctggagatg agcgattccg acttctggat tctggcccgg acattcaagg
     1141 atattctcac ccgtttgaaa gtggcgctca acgacccgcc gttcaatttc gtgctccaca
     1201 cgcggccgat gtcgccggag caccacacct actaccactg gcatttcgag attatcccca
     1261 agctgacccg ggtggccggg ttcgagtggg gctcggggtt ctacatcaat ccgaccaccc
     1321 ccgagcaggc ggccgcctac ctccgccaga tcgacgtcac cgcgccgccc gcggcggcgc
     1381 cgtccgaaat cagcgagaag gaagtataga catgaaggtc tgtttggtgg gaccggagat
     1441 ggttccgttc gcgaagacgg gaggacttgg cgacgtggtg ggggcgctgc ccaaagccct
     1501 cgggcgcctc ggccacgacg tcaccgtctt catgccgctc taccgcgagc tcgacttcgg
     1561 cgtccaccgc gtccacgcgg tcgacctcgc tgtgtccgtt cccgtggccg ggtccgtgca
     1621 aaccgtctcg ctccgcgccg tgcgccagaa aaagcacaac gtcgaaatct acttcatcgg
     1681 caaccgccag tatttcgacc gcgacgggtt ctacgtcgat cccgcgaccg gcaaggacca
     1741 cgccgacaac gacgaacgct tcatcttctt caatcgcgcc gtgctcgaag tgctcaaagc
     1801 gctcaacctc cggccccacg tgatcaacgt caatgactgg cagacggcac tcatcccggt
     1861 ctacctgagg accctcggcg cggccgaccc gtttttcgcc ggcgtcaaga ccgtcctcac
     1921 cattcacaac ctcgcctacc aggggagctt cccccgggag cggtttgcca ggctggagct
     1981 gcccggggcg ctcttcgccc cggcggcggg gccgttcgaa ttctacggcc aggtcaattt
     2041 cctcaaagcc ggtatcacct tcgccgacaa gatcacgacc gtctcgaagc agtacgcccg
     2101 ggagatccag accgccgaat tcggcgccgg tctcgaaggg gtgctggccg ggcgggccgc
     2161 cgacctcgtg ggcattcaaa acggcgtcga ctacaccgtc tggtccccct cgcgcgacaa
     2221 gaaaatccac tacacctatc acctggccaa cctctccggc aagcggatga acaaggtcga
     2281 gctgctcaac cgggcgg
//
LOCUS       DAOOKS010000316         2287 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_58653_length_2287_cov_3.833781, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000316 DAOOKS010000000
VERSION     DAOOKS010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58653_length_2287_cov_3.833781"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..978)
                     /locus_tag="PK112_05755"
     CDS             complement(<1..978)
                     /locus_tag="PK112_05755"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HPC11587.1"
                     /translation="MAKNLVIVESPAKSKTLARFLGKDFDIMATIGHVMDLPKSKLGV
                     DVKNNFQPDYTVIEGKQKVIAELKKAAKKAETVYLAPDPDREGEAIAWHVANSIGNSH
                     KAKFRRVTFNEITKGAVLEAIANPREIDMNLVNAQQARRVLDRIVGYTVSPFLWKTVA
                     RNLSAGRVQSVALRLVCEREAEINAFVSQEYWQLTAELETAGKERLEARLYKIGERSV
                     VKPGDSNDNGNKMSIGSENEVRGYMAELEKESWVVAEIKNTERIRRPSAPFITSTLQQ
                     EAAKAFGFSPKVTMRLAQELYEGVDIGQEGPTGLITYMRTDSTRIANEAL"
     gene            complement(1025..1246)
                     /gene="rpoZ"
                     /locus_tag="PK112_05760"
     CDS             complement(1025..1246)
                     /gene="rpoZ"
                     /locus_tag="PK112_05760"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HPC11588.1"
                     /translation="MKKVPIEQIEKTGLNRYEAVIVASKYARKLNTKRLTLLEQMEDN
                     PEIDIDSRKITMVALNDLIEGKVKFERPR"
     gene            complement(1281..1925)
                     /gene="gmk"
                     /locus_tag="PK112_05765"
     CDS             complement(1281..1925)
                     /gene="gmk"
                     /locus_tag="PK112_05765"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HPC11589.1"
                     /translation="MWNNQGDGILMTPTGKGKVVIISSPSGGGKSSICRQLLERNPAW
                     RFSVSYTTRSRRPDEVHGREYFFVSADEFRRLAKQDFFAEHCRVHLYEYGTPKKPLED
                     VRAEGGVVLLDIDVQGAEKIKAVFADAITIFILPPSIAALRSRLSTRGTETQEQLQVR
                     FENATREMRLWRRFEYAVINDTLATAVREVESAIIAHHCRTDRLAPEQIENIIG"
     gene            complement(1915..>2287)
                     /locus_tag="PK112_05770"
     CDS             complement(1915..>2287)
                     /locus_tag="PK112_05770"
                     /inference="COORDINATES: protein motif:HMM:NF019940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1732 domain-containing protein"
                     /protein_id="HPC11590.1"
                     /translation="TRAVEKEAAQSVDRYRDRLLVRISELAETIQLDPNRLEQEVALQ
                     AERSDITEECTRMFSHIDQYDADLRRDDPVGKRLNFILQEMNREANTIASKCTEASIT
                     RLVISMKEEIEKLRELVQNVE"
BASE COUNT          376 a          655 c          742 g          514 t
ORIGIN      
        1 gagggcctcg ttggcgatgc gcgtcgagtc ggtgcgcatg taggtgatca gaccggtcgg
       61 cccctcctgc ccgatatcga ccccctcgta gagctcctgg gccaggcgca tcgtgacttt
      121 gggcgaaaac ccgaacgcct tggccgcctc ctgctgaagg gtcgaggtga tgaagggcgc
      181 ggaggggcgg cggatgcgct cggtgttctt gatctcggcg acgacccagg attctttctc
      241 cagctccgcc atgtagccgc gcacctcgtt ctcggagccg atcgacatct tgttgccgtt
      301 gtcgttggaa tcgccgggtt tgaccacgct ccgctcgccg atcttgtaga ggcgggcctc
      361 gagacgctcc ttcccggcgg tctcgagttc ggctgtgagc tgccagtatt cctgggagac
      421 gaaagcgttg atctcggctt cgcgctcgca cacaaggcgg agggcgaccg actgcacgcg
      481 cccggccgag aggttgcggg cgaccgtctt ccagaggaac ggggagacgg tgtatccgac
      541 gatgcggtcg aggacgcggc gggcctgctg ggcgttgacg agattcatgt cgatctcgcg
      601 cggattggcg atcgcctcga ggactgcgcc cttggtgatt tcgttgaaag tgacgcggcg
      661 aaacttggcc ttatggctgt tgccgatcga gttggcgaca tgccaggcaa tcgcctcgcc
      721 ctcgcggtcg gggtcgggag cgagatagac ggtctcggcc ttcttggcgg ccttcttgag
      781 ctcggcgatg accttctgct tcccctcgat caccgtgtag tccggctgga agttgttctt
      841 gacgtcgacg cccagcttgg acttggggag gtccataacg tggccgatcg tggccatgat
      901 gtcgaaatcc ttgccgagga agcgtgcgag ggttttcgac ttggccggag actcgactat
      961 gaccaggttt ttcgccatac cttcaatgtt gtcggaacgg gatgccggaa attaacgggt
     1021 gaactcagcg ggggcgttcg aatttgacct tcccctcgat taagtcattc agcgccacca
     1081 tggtaatctt ccgggagtcg atgtcgatct ccgggttgtc ttccatctgc tccaacaggg
     1141 tgaggcgctt ggtgttgagc ttgcgcgcat acttggaggc gacaatgacc gcttcataac
     1201 gattgaggcc ggttttttcg atctgctcga tcgggacttt cttcacgcct gtctccttta
     1261 ccggttccgc accggtcgta ttaaccaatg atgttttcta tttgttccgg cgccaggcgg
     1321 tcggtacggc agtggtgggc gatgatggcc gactcgacct cgcgcacggc ggtcgcaagg
     1381 gtatcgttga tcacggcgta ctcgaaacgc cgccacaggc gcatctcccg ggtggcgttt
     1441 tcgaaccgca cctgaagctg ttcctgcgtc tcggttccgc gcgtgctcag gcggctgcgc
     1501 agcgccgcga tcgacggggg gaggatgaag atcgtgatcg cgtcggcaaa caccgccttg
     1561 atcttctccg cgccctggac atcgatatcg aggagcacga cgccgccctc ggcccgcacg
     1621 tcctccagcg gtttcttcgg cgtcccgtat tcatacaaat gaacacggca gtgttccgcg
     1681 aaaaaatcct gcttggccag acggcgaaat tcgtcggccg agacgaagaa atactcgcgg
     1741 ccgtggactt catccggccg gcggctgcgc gtggtgtagg agaccgagaa ccgccaggcg
     1801 gggtttcgct ccaggagctg gcggcaaatc gaggacttgc cgccgcccga gggggatgaa
     1861 atgatgacga cttttccttt tccggtcggg gtcatgagga tgccatcgcc ctggttattc
     1921 cacattttgc acgagttcgc gcagtttctc gatttcttct ttcatggaga tcaccaggcg
     1981 ggtaatcgag gcctcggtac acttggaggc aatggtgttg gcttcgcgat tcatctcctg
     2041 gaggatgaag ttgagccgtt tgccgaccgg atcatcgcgc cgcaggtcgg cgtcatactg
     2101 gtcgatgtgg ctgaacatac gggtgcactc ctcggtgatg tcggagcgct cggcctggag
     2161 ggccacttcc tgttccaggc ggttggggtc aagctgaata gtttcagcga gttccgagat
     2221 tctgacgagg aggcggtcgc ggtagcggtc gacactctgg gcagcctcct tctcgaccgc
     2281 ccgggtg
//
LOCUS       DAOOKS010000317         2285 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_58736_length_2285_cov_2.820179, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000317 DAOOKS010000000
VERSION     DAOOKS010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58736_length_2285_cov_2.820179"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="PK112_05775"
     CDS             complement(<1..189)
                     /locus_tag="PK112_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HPC11591.1"
                     /translation="MIDKFRSVFKVEELRRRIFYTLALLVVYLIGGHIPTPGIDGSIL
                     ASVLANNTIFGLFDLFVGG"
     gene            complement(186..626)
                     /gene="rplO"
                     /locus_tag="PK112_05780"
     CDS             complement(186..626)
                     /gene="rplO"
                     /locus_tag="PK112_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HPC11592.1"
                     /translation="MELHTLKPPKGARKTRRRVGRGSGSGLGKTSGRGHKGAGARSGF
                     KREAWREGGQMPLARRLPKFGFTNIFKKTWQVINLSDLARLEAGEVTPDTMLAAGLIK
                     TTAVPVKVLGDGTVEKAYTVKAAAFSKSAASKIEAAGGKAEVLS"
     gene            complement(636..824)
                     /gene="rpmD"
                     /locus_tag="PK112_05785"
     CDS             complement(636..824)
                     /gene="rpmD"
                     /locus_tag="PK112_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HPC11593.1"
                     /translation="MGKLRVTQIRSTIDRKEPHKRTIEALGLGKIRRSVIHNDTPQIR
                     GMIRAVRYLVTVEEIDEK"
     gene            complement(851..1333)
                     /gene="rpsE"
                     /locus_tag="PK112_05790"
     CDS             complement(851..1333)
                     /gene="rpsE"
                     /locus_tag="PK112_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009353893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HPC11594.1"
                     /translation="MARFEMENLELEEKVISVKRVAKVVKGGRRFSFTALVAVGDKNG
                     RVGVGHGKAPEVSDAIRKGTEAARKNMTSVNLLKGTIPHRIDGEFGATRVVMRPASPG
                     TGVIAGGAVRAVLESVGIQDILAKTLGSRNPHNMVKATLNGLAALQTREQEVKARQAE
                     "
     gene            complement(1372..1746)
                     /gene="rplR"
                     /locus_tag="PK112_05795"
     CDS             complement(1372..1746)
                     /gene="rplR"
                     /locus_tag="PK112_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004560259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HPC11595.1"
                     /translation="MAGKSVKKFKRAERRRQRVRGKLSGTSACPRLTVAKSLKNVFVQ
                     IVDDVHAVTLAAAASNSKNVLSELKPEMTKSEVARKVGEVIAREAKAKGIEAVVFDRN
                     RFRFHGRVKAVAEGARSGGLKF"
     gene            complement(1761..>2285)
                     /gene="rplF"
                     /locus_tag="PK112_05800"
     CDS             complement(1761..>2285)
                     /gene="rplF"
                     /locus_tag="PK112_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HPC11596.1"
                     /translation="KQPIAVPDKATVSITGRQVTIKGPKGSLTLEVHPDMTASLDHNQ
                     IVVARPSDLKKHRALHGLTRALLHNMVVGVTQGFTRELEIVGVGYRAENRGRHLVMYL
                     GYSHPVVLTPPEGVTVEAVPKEGKVRVSGIDKQLVGQVAAKIRSFRKPEPYKGKGVRY
                     AGEHVRTKAGKTAG"
BASE COUNT          319 a          814 c          667 g          485 t
ORIGIN      
        1 cccgcccacg aacaggtcga acagcccgaa gatggtgttg ttggccagca ccgaggccag
       61 gatgctgccg tcgatcccgg gcgtggggat gtgtccgccg atgaggtaga cgacgagcag
      121 cgcgagggtg tagaagatcc gtcttcgcaa ctcctcgact ttgaacaccg atctgaactt
      181 atctatcaag acagcacctc ggcttttccg ccggcggctt cgatcttgct ggccgccgac
      241 ttactgaacg ccgccgcttt gaccgtgtac gccttctcca ccgtcccgtc ccccagcact
      301 ttcaccggta cggccgttgt cttgatcagg cccgccgcga gcatcgtatc gggcgtcacc
      361 tcgcccgcct cgaggcgcgc gaggtcggac aggttgatca cctgccaggt cttcttgaag
      421 atattggtga aaccgaattt gggcaggcgc cgtgcgagcg gcatctggcc gccttcgcgc
      481 caggcttcgc gcttgaagcc gctccgcgcc ccggccccct tgtggccgcg tcccgaggtt
      541 ttccccaggc cgctgcccga gccgcgtccc acccgcctgc gcgtctttct tgcccctttc
      601 ggcggtttca acgtatgcag ttccatcact cgcctctatt tttcgtcgat ctcttccacc
      661 gtcacgagat agcggacggc gcggatcata ccgcggatct gcggcgtgtc gttgtggatg
      721 acggaccgcc ggattttgcc gagccccagc gcctcgatgg tgcgtttgtg cggctccttg
      781 cggtcgatcg tgcttctgat ctgcgtcact ctaagcttgc ccattgcggt cgtcctctct
      841 gccggcacgc ctactcggcc tggcgcgcct tgacttcctg ttcgcgcgtc tgcagggcgg
      901 ccaacccgtt cagcgtcgcc ttgaccatgt tgtgcgggtt gcggctcccc agcgtcttgg
      961 ccaggatgtc ctgaataccc accgactcga gcacggcccg cacggcgccg ccggcgatca
     1021 cgccggtgcc gggcgaggcc ggccgcatga ccacccgggt ggcgccgaac tcgccgtcga
     1081 tccggtgcgg gatcgtcccc ttgagcaggt tcacggaggt catgttcttg cgcgccgcct
     1141 ccgtcccctt gcggatcgca tcggatacct cgggggcctt gccgtggccc acgccgaccc
     1201 ggccgttctt gtcgccaacc gccaccagcg cggtaaacga aaaccgccgg ccgcccttga
     1261 ccactttcgc cacccgcttg accgagatta ccttttcctc gagttcgagg ttttccatct
     1321 caaaacgcgc caatctgcac ctcagcttgt cctatgcaca cttattcgtt atcagaactt
     1381 caggccaccc gagcgcgccc cctccgcaac cgctttgacc cggccgtgga agcggaatcg
     1441 gttgcggtcg aagaccaccg cctcgatccc cttcgccttc gcctcgcgcg cgatcacttc
     1501 gcccactttc cgcgccactt cggacttggt catctccggc ttgagctcgg agaggacgtt
     1561 tttggaattg gaggcggccg ccgcgagcgt caccgcgtgg acgtcgtcga caatctgcac
     1621 gaacacgttc ttcagcgact tcgcgaccgt caggcgcggg cacgccgagg ttcccgacag
     1681 cttgccccgc acccgctggc ggcggcgttc cgcccgcttg aacttcttga cactctttcc
     1741 agccatttcc gtctactctc ttatccggcg gtcttcccgg ccttcgtccg cacgtgttcg
     1801 ccggcgtacc ggacaccctt gcccttgtac ggctccggtt tccggaacga ccgaatcttc
     1861 gccgccacct ggccgacgag ttgcttgtct atccctgaga ccctgacttt gccctctttg
     1921 ggcacggctt cgacagtcac gccctccggc ggggtgagga ccaccgggtg cgagtacccg
     1981 aggtacatca cgaggtgccg gccgcggttt tcggcgcggt agccgacgcc gacaatttcg
     2041 agttcgcggg tgaacccctg ggtcactccc acgaccatgt tgtgcaggag cgcccgcgtg
     2101 aggccgtgga gggcccggtg tttcttcaga tcgctcgggc gggcgacgac aatctggttg
     2161 tggtccaggc tggccgtcat gtccgggtgc acctccagag tgaggctgcc cttcggcccc
     2221 ttgatcgtca cctggcgccc ggtgatgctc accgtcgcct tgtccggcac agcgatcggc
     2281 tgctt
//
LOCUS       DAOOKS010000318         2282 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_58840_length_2282_cov_3.224068, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000318 DAOOKS010000000
VERSION     DAOOKS010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58840_length_2282_cov_3.224068"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..939)
                     /locus_tag="PK112_05805"
     CDS             complement(151..939)
                     /locus_tag="PK112_05805"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPC11597.1"
                     /translation="MRRSRLTALLLLSPWILSFAVFWLFPLVYSLVMGFTDYRLLAPA
                     WRWIGLANYRSLLADESFLAAVENTFIFVLGTVPVTTVIALGLALLVNRQFPGRGLFR
                     AGYFIPSITSMVVVALIFTNLYQRGGYIAALAAMVGLPAPEHGFLFSDKTALASIMAM
                     DVWMAVGYYMLIFLAGLKSIPDELYEAARIQGAGRARQFFSITLPLLRPVALFIVVIN
                     SIKSFQVFARVENLLDQAYSTEPGFPMPARSFKAGLRFDYDFTR"
     gene            complement(936..2147)
                     /locus_tag="PK112_05810"
     CDS             complement(936..2147)
                     /locus_tag="PK112_05810"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPC11598.1"
                     /translation="MRIRATILAAALLLAGPARAATVEWWQFWTDPAIKPVIEQMVAE
                     FEQANPDIKVRLTDLTWANGHEKIVLAFSSGTAPDVVELGSDWIAQFAANGRLADLSG
                     LVAGDTAGYDGWSMAADRGGLYAWPWILGTRVLFGNRDLITRAGYDTAFYPVNWPQLT
                     EAAKKVRALGPNFYGWGSNTAEKHRLYKKYLPFFWSAGAQLFSDDGRMCVVASSRAVS
                     ALRTYQQLHDSAGYVDTQRGIEDAFLDGRIGFIISGDWLLKRIELENRRINLISTLIP
                     GPKFPGRSFLGGEFLAVSADSDVKEAALRLIRFVTSPVNQVRFCRANRSANPSSREAQ
                     RDPYFASNPHLQTFIAALKSAQHPPVDPDWVYIEDILEKAVEEALFGSHLVAVPLLEA
                     RYKIEELKGLK"
BASE COUNT          453 a          680 c          781 g          368 t
ORIGIN      
        1 cctccccaat ccttcaaaaa cccatgcgcg agggacggac cgaacgagga ggcgttgaaa
       61 catccgggta gccggtcgtt tccttcggac agatcccgcc ggtcgtttgc tcaaccggcg
      121 tgcgccgcct cctccgtccg ccggtacttg tcagcgtgtg aaatcgtagt cgaaacgcaa
      181 accggccttg aatgaccgcg ccggcatcgg gaatcccggt tccgtggagt aggcctggtc
      241 cagcaggttc tccacccggg cgaagacctg gaacgacttg atcgagttga tgacgacgat
      301 gaagagcgcg accgggcgga gcagggggag ggtgatggag aagaactggc gcgcgcggcc
      361 ggccccctgg atgcgggcag cctcgtacaa ctcgtcggga atcgacttca ggccggcgag
      421 aaagatgagc atgtagtacc cgaccgccat ccagacatcc atcgccatga tggaggcgag
      481 cgcggtcttg tcgctgaata ggaaaccgtg ctccggcgcc ggcaggccga ccatcgcggc
      541 gagggcggcg atataccctc cccgttggta gaggttggtg aaaatcaggg cgacgacaac
      601 catggaggtg atcgagggaa taaaatagcc ggcgcggaag aggccgcgtc cgggaaactg
      661 ccggttcacc aggagagcga ggccgagcgc gatgacggtg gtgacgggca cggtcccgag
      721 gacaaagatg aaagtgttct ccaccgcggc gaggaagctc tcgtcggcca gcaggctccg
      781 gtaattggcc aagccaatcc agcgccaggc cggggcgagg agacggtagt cggtgaaccc
      841 catcaccagc gagtacacca ggggaaacag ccagaacacc gcgaagctga gaatccaggg
      901 ggacaggagg agcagggcgg tgagccgcga gcgcctcatt tgagcccctt caactcctcg
      961 atcttgtacc gggcctccag cagcggcacg gccaccaggt gcgacccgaa gagcgcctcc
     1021 tccacggctt tctcgagaat gtcctcgata taaacccagt cgggatcgac cggcggatgc
     1081 tgggcggact tgagggctgc gatgaaggtc tgcaggtggg ggttggaggc gaaataggga
     1141 tccctttggg cctcgcggct cgaggggttg gccgaacggt tggcccggca gaaacggacc
     1201 tggttgaccg gggaggtgac gaagcggatc agcctcaggg cagcctcctt cacgtcggag
     1261 tcggcgctca cggccaggaa ctcgccgccg aggaacgagc gcccggggaa tttcgggccg
     1321 gggatcagcg tcgatatcaa gttgattcgc cggttctcca gctcgatccg cttgagcagc
     1381 cagtccccgg aaatgatgaa cccgatgcgt ccgtcgagga aggcgtcctc gataccgcgc
     1441 tgggtgtcga catagccggc ggagtcgtgc aattgctggt aggtccggag ggcactgacg
     1501 gcgcgcgagg aggccaccac gcacatccgg ccgtcatcgg aaaacaactg ggcgcctgcc
     1561 gaccagaaaa acggcaggta cttcttatag aggcggtgct tttcggccgt gttggacccc
     1621 caaccgtaga agttggggcc gagcgcccgg actttcttcg ccgcctccgt cagttgcggc
     1681 cagttgaccg ggtagaaggc agtgtcgtag ccggcgcggg tgatcaggtc gcggttgccg
     1741 aagaggacgc gggtgccgag aatccagggc caggcataca gcccgccgcg gtcagcggcc
     1801 atgctccagc catcgtaccc ggcggtgtcg ccggcgacca ggccggaaag atcggcgagc
     1861 cgcccgttgg cggcgaactg ggcgatccag tcggagccga gttccaccac gtccggggcc
     1921 gtgcccgagg agaaagccag gacgattttc tcgtggccgt tcgcccaggt gagatcggtc
     1981 aggcgcactt tgatgtcggg gttggcctgt tcaaactcgg ccaccatttg ttcgatgacc
     2041 ggtttgatgg ccgggtcggt ccagaactgc caccattcca ccgtggcggc gcgggccggg
     2101 ccggccagaa ggagcgcggc cgcaaggatc gtcgcacgta ttctcatcca cctacctctg
     2161 catccagaca cgtcgtctcg cagcggcatg aaacggatcg ccggcgtccc cggtcaacca
     2221 aaataatctt ttagttgttt cgacgcaggc ggaggtcttt ccatgtgccg tgaacatggc
     2281 gg
//
LOCUS       DAOOKS010000319         2274 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_59139_length_2274_cov_3.188373, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000319 DAOOKS010000000
VERSION     DAOOKS010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59139_length_2274_cov_3.188373"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1021)
                     /locus_tag="PK112_05815"
     CDS             complement(<1..1021)
                     /locus_tag="PK112_05815"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HPC11599.1"
                     /translation="MKPLRVTSRILKRFLESPTEILIVLSVFVGLVTALGALAFRALI
                     DFANHLFFGLTDQVLTKSLGAGSFKWWLPIVPALGGLIVGAIVLRFAAEARGHGVPEV
                     MNAVARLGGIIRPRVAAVKTIASAICLGSGGSAGREGPIVQIGSALGSTIGQLFRMSG
                     DRIKILVGCGAAAGISSVFNAPIAGVIFTLEVVLGDFALATFSPVIISSVVASVVTRS
                     VLGANPAFAVPTFALVSGWEMPLYALLGVLLGGVGFVFTRMMVACEEFFEKMAIANWL
                     KPALGGLLLGGIAVFFPQVLADGYGTIRLTLQGEMALWLMAALVFLKILATSLTLGSG
                     NSGGIF"
     gene            1214..2143
                     /locus_tag="PK112_05820"
                     /pseudo
     CDS             1214..2143
                     /locus_tag="PK112_05820"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
BASE COUNT          473 a          722 c          731 g          348 t
ORIGIN      
        1 cgaagatccc cccggagttg cccgacccga gagtgagcga ggtggccagg attttgagaa
       61 agaccagcgc cgccatcagc cagagcgcca tctcaccctg cagcgtcagc ctgatcgtcc
      121 cgtatccgtc ggccagcacc tgcgggaaaa agacggcgat tccgccaagg agcagtcccc
      181 ccaatgcggg tttgagccaa ttggcgattg ccattttctc gaagaactcc tcacaggcga
      241 ccatcatccg cgtgaagacg aaaccaaccc cgccgaggag cacgccgagc agggcgtaga
      301 ggggcatctc ccagcccgag acgagggcga aggtgggcac ggcgaaagcg ggattcgcgc
      361 ccaggaccga acgggtcacc accgaggcga cgacggagga gatgatcacc ggggagaagg
      421 tggcgagcgc gaaatccccg aggacgacct cgagggtgaa aatcacgccg gcaatggggg
      481 cgttgaaaac cgaggaaatg ccggcggccg ccccgcaccc caccaggatt ttgatgcggt
      541 cgcccgacat gcggaagagc tgaccgatcg tcgaccccaa ggccgaaccg atctggacga
      601 tcggtccctc ccgtccggcc gaccccccgg acccgaggca gatggccgag gcgatcgttt
      661 tgacggctgc gacgcggggc cgaatgatac ctccgagccg ggcgacggcg ttcatcactt
      721 cgggcacacc gtggccccgg gcctcggccg cgaaccggag cacgatcgcc cccacgatca
      781 ggccgccgag ggccgggaca atcggcaacc accacttgaa cgaccccgcc ccgaggcttt
      841 tggtcagcac ctggtcggtg agaccgaaga acaggtggtt ggcgaagtcg atcagggccc
      901 gaaaggccag cgcccccaaa gccgtaacca gcccgacaaa taccgagagc acaatcagga
      961 tttcggtcgg cgactccagg aaccgcttga ggatccggct cgtgacgcgg agcggtttca
     1021 tacgggcgca ataacgggcc ggcctatgcg ggggcacaag tttttagcgc ccgacccgcg
     1081 cgaaaaaagc cttctgccca cggggacttc cccgccgacc aacccggaaa gcgcttgaca
     1141 actcccccca attcaatacc ttcaacgtga aaaagttcac tatgtttgca gacattgcag
     1201 tgaggacgac atcatgcgca gtcatgccga cgcggtcatc gtaggggggg gcatcatcgg
     1261 cctctcggtt gcgttccacc tcgcccgcga gaagtacggc cggatcatcg tgctggaaaa
     1321 ggagcagttg ctgggcaccg gggccacgtc caaggcggcg ggcggcatcc gcgcgcagtt
     1381 ttccaccaaa gtcaatatcg agatgtccat gctctcggag aagctgttcc gccagttcaa
     1441 ggaggatacc ggctcggacg ccctgttcga tcaggttggg tacatgttcc tgctgcagac
     1501 ggaggcggag gtccgggagt ttacgaaaag ctatgagttg cagcgctcgc tcggccttcc
     1561 cgccgtgctg ctgaaaacgg atgagatccc gcagtatgcc ccgcacatca agctcgacgg
     1621 ggtgcggatg gcgaccttct gcaaggatga cggtctgggg gatccgcacg aatttctcag
     1681 cggctacgag catgccgcgc gccggctcgg agtggaaatc gcctttgagt cgccggtgac
     1741 gggtattcag gtcgagtcgg gccggatccg gtcggtgcag acggcggcag gccgcatcga
     1801 caccccgctg gtggtcaact gcgccggacc gcagtcccgc gaggtggcgg cgatggccgg
     1861 ggccgagctg ccggtcgatc caattcgccg ccagattgtc acgaccggcg aactcagctg
     1921 ggtggaaccg tatttcccga tggtggtcga cgtctcgtcc ggcctctatt tccataagga
     1981 gtcgaaaggg atgctcttcg ggtgggccaa accgggcgtc aagccctcat acgacatctc
     2041 ggtcgatccc gagtacaccg acgagatcct cgagcggacg atgatccgca tcaaggacga
     2101 gctgttggac gagatcgcgg tggccaacca gtggggcggc ctgtacgaga cgacccccga
     2161 tcaccacgcg gtcatcggcc gggagccccg ggtcgacggc ctgaatcagc gaggtcaagc
     2221 cgccgcacag caccacctgt tcgccgaaca gatcggtttc gcactcttca cgga
//
LOCUS       DAOOKS010000320         2272 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_59220_length_2272_cov_2.835814, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000320 DAOOKS010000000
VERSION     DAOOKS010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59220_length_2272_cov_2.835814"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="PK112_05825"
     CDS             complement(<1..322)
                     /locus_tag="PK112_05825"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPC11600.1"
                     /translation="MDLGLNGKRALVTGASSGLGLACAAALAAEGAAVTINSRNRERL
                     AAAAEKIAAATGAPATLAVGDVADPDDVRRIVQTAGEVDILVSNCGGPAAGPCGARPR
                     RPWSR"
     gene            complement(322..1695)
                     /locus_tag="PK112_05830"
     CDS             complement(322..1695)
                     /locus_tag="PK112_05830"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HPC11601.1"
                     /translation="MTPLDYLTAHEKDRMDQLFEFLRFPSVSAKSAHQPDMQACAQWL
                     ADHFTGLGFRAAVHPTGGHPLVYAEYAVDRRLPTVLYYGHYDVQPPEPLNLWQSPPFQ
                     PEIRDGMICARGATDDKGQLFAHIKGLEAILRSEGRLPVNVKFLVEGEEEVSSANLPK
                     WLKANKKLLAADIVVVSDSSQFSKTLPAVTYGLRGIVAMELFVLGPKRDLHSGTFGGA
                     VANPANILCQIVGRLHDANGRITVPGFYKDVAPPAKWERAQFKKLPFSEAQYMKMVGI
                     PALHGERGWNTFERTWVRPTLDVNGITAGYQGEGAKTVLPSYASCKITCRLVPAQKPT
                     DILQKVEAYIRKIAPKSVRIELKKSGGAPAVVVPTDGPWLEAAARALKKGFGKAPVFI
                     REGGSIPIVGDFKEILGLDTLLLGFGQNDDNTHSPNEHFRVVDFERGCRTAAVLPHEL
                     ARAAEAK"
     gene            complement(1811..>2272)
                     /locus_tag="PK112_05835"
     CDS             complement(1811..>2272)
                     /locus_tag="PK112_05835"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HPC11602.1"
                     /translation="RLETEGRAFFEEAARKAESDLVRQTFQFLAAEEIHHIEKIDKFV
                     RSLEISEGREVPEIEDSNAEAKLAEFNNRLAALRDTISPSASDVEAYRFAIKFENGAE
                     EFYLEKMNEAEDPRIKRFYKWLIDEETMHARLLRSCLNFACDPAEWFRRHR"
BASE COUNT          360 a          764 c          740 g          408 t
ORIGIN      
        1 aacgggacca tggacgccga ggtcgagcgc cacacggccc cgccgccgga cccccgcagt
       61 tggagaccaa aatgtcgact tccccggcgg tctgcacgat ccgccggacg tcgtccggat
      121 cggcgacgtc gccaaccgcg agcgtcgccg gcgccccggt cgcggccgcg atcttctcgg
      181 ccgcggccgc cagccgctcc cggttccggc tgttgatcgt gacggccgcg ccttcggcgg
      241 ccagggccgc ggcgcacgcc aggccgagtc ccgaggaggc cccggtgacc agcgcccgtt
      301 tgccgttgag gccgagatcc atcacttcgc ctcggccgcg cgcgcgagtt cgtggggaag
      361 cacggccgcg gtccggcagc cccgctcaaa atccacaacc cggaaatgct cgttcgggga
      421 atgggtgttg tcatcgttct ggccgaaccc gagcaggagc gtgtccaggc cgaggatttc
      481 cttgaaatcg cccacgatcg gaatcgagcc gccttcccgg ataaacaccg gggccttgcc
      541 gaaacccttt ttgagcgcgc gcgcggccgc ctccagccac gggccgtcgg tcggcaccac
      601 gaccgcgggc gcccccccgg atttcttcag ctcgatccgc accgacttcg gcgcaatctt
      661 cctgatgtac gcctcgacct tctgcagaat atccgtcggc ttctgcgccg gcaccagccg
      721 gcaggtgatc ttgcaggagg cgtacgacgg cagcactgtc ttcgcccctt ccccctggta
      781 cccggccgtg atcccgttca catcgagcgt cggccgcacc caggtgcgtt cgaaggtgtt
      841 ccacccccgc tcgccgtgca gcgccggaat gccgaccatc ttcatgtact gcgcctcgct
      901 gaagggcagc ttcttgaact gggcgcgttc ccacttggcg ggcggggcga cgtctttgta
      961 gaaccccggc accgtgatcc ggccgttcgc gtcgtgcagc cgtccgacaa tctggcagag
     1021 aatattggcg gggttggcga ccgcgccgcc gaacgtgccc gaatgcagat cgcgcttggg
     1081 gccgagaacg aacagctcca tggcgacgat gccgcgcaga ccgtaggtga ccgccggcag
     1141 cgtcttgctg aactggctgg agtcggagac gacgacgata tcggcggcca aaagcttctt
     1201 gttcgccttc agccacttgg gcagattggc cgacgagacc tcttcctctc cctcgacgag
     1261 gaatttcacg tttaccggca gccgcccctc gctcctgagg atcgcctcca gtcccttgat
     1321 gtgggcgaac aactgcccct tgtcatcggt ggcgccgcgg gcgcagatca tgccgtcgcg
     1381 gatttccggc tgaaagggcg ggctttgcca gagattgagc ggttccggcg gctggacgtc
     1441 gtagtggccg tagtagagga cggtcgggag gcgccggtca accgcgtatt cggcgtacac
     1501 gagcgggtgc ccgccggtcg ggtgcaccgc cgcccggaaa cccaggcccg tgaagtggtc
     1561 ggcgagccac tgggcgcacg cctgcatgtc cggctggtgc gcggatttgg ccgagaccga
     1621 agggaagcgg agaaactcga acaactggtc catgcggtct ttctcgtgcg cagtgaggta
     1681 atcaagcggg gtcatggcat tactccttgg tttgcattcc gcagtcaacg cagggagaat
     1741 atagggcgcg cgcggggcgg tgtaaagccc cggctgaccg gcaggaaccg ccccgctgcc
     1801 ggctgcgcgg ctaccggtgc cggcgaaacc actcggctgg atcgcaggcg aaattcaggc
     1861 aggaccgcag cagccgggcg tgcatcgtct cctcgtcgat cagccacttg tagaagcgct
     1921 tgatccgggg gtcctccgcc tcgttcattt tctcgaggta gaactcctcg gctccgtttt
     1981 cgaatttgat cgcaaaccgg taggcctcga cgtcggaggc gctcggggag atggtgtccc
     2041 ggagcgcggc gaggcggttg ttgaattccg ccagcttcgc ttcggcgttg gaatcctcga
     2101 tctccggcac ctcccggccc tccgatattt ccagcgaccg gacgaatttg tcaattttct
     2161 cgatgtggtg gatttcctcg gcggcgagaa actggaaggt ctgccggacc aggtcgctct
     2221 ccgccttgcg ggcggcctct tcgaaaaacg cccggccctc ggtctcgagc cg
//
LOCUS       DAOOKS010000321         2250 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_60089_length_2250_cov_2.759453, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000321 DAOOKS010000000
VERSION     DAOOKS010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60089_length_2250_cov_2.759453"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1248)
                     /gene="selB"
                     /locus_tag="PK112_05840"
     CDS             complement(<1..1248)
                     /gene="selB"
                     /locus_tag="PK112_05840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HPC11603.1"
                     /translation="MIVVGTAGHIDHGKSALVKRLTGTDPDRLPEEKARGMTIDLGFA
                     FYRTPAGATVAFIDVPGHERFVKNMIAGAGGIDAVMLVIAADDGWMPQTEEHFQVVRL
                     LGVRHGLVVINKTDLVEPDWLELLTADIGARLAGTFLHDVPVFAVSAATGAGFDPLRE
                     YLNRLPGLVASRKDIGKARLFIDRSFVRPGIGGVVTGTLRGGSLRLGQTVGVWPARTV
                     GKIRSLHSGGDSVDLAEPGQRTAVSFTGVDREQLLRGGVITDRTDLEYFPHHPVLALS
                     LELLPNAPVALEDRRRVLLIVGTTEVEGEIRLFRAEEIRPGGSGLVFFQPDQPVYALI
                     GDRCIMRLPTPMVTLGGSQVLDHLRFFPRRRALDRYDYLLLRSSGRLEDLVRSELQKR
                     TAVHRPHLLEHADCAETAINQAVT"
     gene            complement(1255..>2250)
                     /gene="selA"
                     /locus_tag="PK112_05845"
     CDS             complement(1255..>2250)
                     /gene="selA"
                     /locus_tag="PK112_05845"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HPC11604.1"
                     /translation="RGGAAESYLALLAEAEAATVVNNCAAALFLALNSLAARKEVLIS
                     RGELVQIGGGFRIPDILRKSGARLREVGTTNITTIADYASNIDPKRTGLILKVHKSNF
                     TQAGFTEEVSLRDLVALGREHNLPVMNDLGSGLFIDTLPLLGYSEPTVQQSVRAGAVL
                     TTFSGDKLLGGVQAGLLVGRAEIIRTIKKNPLFRTMRVDKIVFSALERLFSIYLGGTW
                     QTDITLWRLLALPESELYKRGKKILADLGQPEDLSVEATQAFVGGGALPEAAIPSVAV
                     CFSKTRNAAALLAAFRDRDLPIIGRIENDRFLLDLKAVETEDLPALTEAIRSVLG"
BASE COUNT          369 a          632 c          893 g          356 t
ORIGIN      
        1 ggtgacggcc tggttgatgg cggtctcggc gcagtcggcg tgttcgagca ggtgcgggcg
       61 gtggacagcg gtgcgtttct gcagttccga acggacgaga tcctccagtc ggccgctgct
      121 ccggaggaga agatagtcgt agcggtcgag ggcgcggcgg cgggggaaga agcggaggtg
      181 gtcgagcacc tggctgccgc cgagcgtgac catcggtgtc ggcaggcgca tgatgcagcg
      241 gtcgccgatg agggcgtaga cgggctggtc gggctggaag aaaaccaggc cggagccgcc
      301 gggccggatc tcctcggcgc ggaagaggcg aatctccccc tcgacctccg tggtgccgac
      361 tatgaggagg acgcggcgcc ggtcctccag ggcgaccggg gcgttgggga gaagttcgag
      421 cgagagggcg agcaccggat ggtgcgggaa gtactcgagg tcggtgcggt cggtgatgac
      481 cccgccgcgg aggagctgtt cgcggtcgac gccggtgaag gagacggcgg tgcgctggcc
      541 cggttcggcg aggtcgacgc tgtcgccgcc ggagtggagc gaccggattt tgccgacggt
      601 gcgggcgggc cagacgccga ccgtctggcc gaggcgcaga gagccgccgc gcagggtgcc
      661 ggtcaccacg ccgccgatgc cgggccggac gaaggagcgg tcgatgaaga ggcgggcttt
      721 cccgatgtct ttgcgcgagg cgacgaggcc ggggaggcgg ttgaggtatt cgcgcagggg
      781 atcgaaaccg gcgccggtgg ccgcggagac cgcaaagacc gggacgtcgt ggaggaaggt
      841 gccggccagg cgcgcgccga tgtccgcggt caggagctcc agccagtcgg gctcgacgag
      901 gtcggtcttg ttgatcacca ccaggccgtg ccgtaccccg agcaggcgaa ccacctgaaa
      961 gtgctcctcg gtctgcggca tccagccgtc atcggcggcg atcacgagca tcacggcgtc
     1021 gatcccgccg gcccctgcga tcatgttctt gacaaaccgc tcgtggccgg gaacatcgat
     1081 gaaggcgacg gtggcgccgg cgggcgtgcg gtagaaggcg aagccgaggt cgatggtcat
     1141 gccgcgcgcc ttctcctccg ggagacggtc ggggtcggtg ccggtgaggc gtttgacgag
     1201 ggccgacttg ccgtggtcta tgtgtcccgc ggtgccaacg acgatcatag ccgattaccc
     1261 gaggaccgac cggatggctt cggtgagagc cggcaaatcc tctgtttcga ccgctttcag
     1321 gtcgaggagg aagcgatcgt tttcgatccg accgatgatg gggaggtcgc ggtcgcggaa
     1381 ggcggccagc agggcggcgg cgttgcgggt tttggagaag cacacggcga ccgaggggat
     1441 ggcggcctcg gggagggcgc ctccgccgac gaatgcctgg gtggcttcca ccgagaggtc
     1501 ctcgggctgc cccaggtcgg ccaggatttt cttaccccgc ttgtacagct cggattcggg
     1561 gagggcgagc aggcgccaga gcgtgatgtc ggtttgccag gtgccaccga ggtagatgga
     1621 gaagaggcgc tcgagggcgg agaagacgat tttgtcgacg cgcatggtgc gaaagagcgg
     1681 gttcttcttg atcgtgcgga tgatctcggc ccggccgacc aggaggccgg cctggacgcc
     1741 gccgaggagt ttgtcgccgg agaaggttgt gagcacggcg ccggcacgaa ccgattgctg
     1801 gacagtcggt tcggagtagc caaggagggg gagggtgtcg atgaagaggc cgctgccgag
     1861 atcgttcatc accgggaggt tgtgctcgcg gccgagcgcg accagatcgc ggagggaaac
     1921 ctcctcggtg aagccagcct gggtgaaatt gcttttgtgg actttcagga taagcccggt
     1981 gcgcttcggg tcgatattag aggcgtagtc cgcgatcgtg gtaatgttcg tggtgccgac
     2041 ttcccggagc cgggcgcctg atttgcggag aatatcgggg atgcggaagc cgccgccgat
     2101 ctggaccagc tcgccccgcg agatgaggac ctccttgcgg gcggccaggg aattgagggc
     2161 cagaaagagc gccgcggcgc agttgttgac aacggtggcg gcctcggcct cggcgagcaa
     2221 ggcgaggtag ctttcggccg ccccgccccg
//
LOCUS       DAOOKS010000322         2249 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_60114_length_2249_cov_3.945761, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000322 DAOOKS010000000
VERSION     DAOOKS010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60114_length_2249_cov_3.945761"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..1695)
                     /locus_tag="PK112_05850"
     CDS             complement(19..1695)
                     /locus_tag="PK112_05850"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HPC11605.1"
                     /translation="MPTLSPDIMNLLLGVAPLPTVEPSGATAGKEAGAPPELLFGALM
                     QTALGPSRVTSLLPLAKTASMPSGTQAASVPGQTARPADDTLAAACRPLVPATRDGER
                     ALLDDLLVSPGWDFEALETTSGDIYDKARSVLQGRAAIQAAAVSDEYSPAATNPPGSI
                     GTGTFVPTPAALQLIQDLTPQPLDTLPAGAYIITDARAAEGRLMLDLSPKDPGHPAIR
                     ISLPLPQPSEIPAPHVSVPASAARVPLESGASPTALESWFEKVSLKELVVTPAAGHGS
                     AQAGGAAASSEAPCKFEILAENAGRDVVIVTTPAGSDIRFRLLQNRAARPASPGTPGA
                     ALAGAKTTPRPAAAAPDTADGHFTDSRLFPLTREIRFLTLAGGRGKAPDASGEAAATL
                     AGSAKGAGADDLRTAAAGERVQTPPARFTLPADVKTALRPGGDSVELRIDPEHLGPAR
                     LHLTVRDEHLSARVIVETARAKHTIENSLDRLVETLARADIKVDHIEVAIGQQGARGQ
                     WFAQHAPHWPRSGASGGRFDRDDETAPAPPAVPATGSSGYLGAGGVDVLA"
     gene            complement(1698..>2249)
                     /locus_tag="PK112_05855"
     CDS             complement(1698..>2249)
                     /locus_tag="PK112_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11606.1"
                     /translation="APGDTDSTLAGNRAAQAAQLTDDQILDSLLAGEDDSDMLALIQE
                     NLNVLDYDPAESAAAGDTAPAIEGAPGTADHLAEEQKRLAQWESQLKTRENDLVRREN
                     EVSRQMLVLEQAESARTAGLAKLYDGMDSHAVAKLMTNLDDETVVSILPRMKAKNASE
                     VLQLLPPERAARLSKQMITIAEK"
BASE COUNT          318 a          728 c          823 g          380 t
ORIGIN      
        1 ttcgctcatg gtcgtctcct atgccaaaac atccaccccg ccggcgccga gatagccgga
       61 cgatccggtc gcggggaccg cgggcggggc gggggcggtt tcatcgtctc ggtcgaagcg
      121 gcctccggaa gcgccggagc gtggccagtg cggcgcgtgc tgcgcaaacc actgcccgcg
      181 cgccccctgc tggccgatcg ccacttcgat gtgatccact tttatgtccg cccgggcgag
      241 ggtctcgaca aggcggtcca gggaattctc aatcgtgtgt ttcgcgcggg cggtctcgac
      301 gatgacccgg gcggagagat gctcgtcgcg caccgtcagg tggagccggg ccgggccgag
      361 atgctcgggg tcgatgcgca gttcgaccga gtcgccgccg gggcggaggg ccgtcttcac
      421 gtccgccggc agcgtgaaac gggcgggcgg cgtctgcacc cgctcgccgg cggccgccgt
      481 gcggagatcg tctgcgccgg cgcctttggc cgacccggcc agggtcgcag ccgcctcgcc
      541 ggatgcatcg ggggccttgc ctctgccccc tgccagcgtg aggaaccgga tctcgcgggt
      601 cagagggaac agacggctgt cggtgaagtg tccatcggca gtgtctggtg cggcggcggc
      661 cgggcgcggc gttgtcttgg cgcccgccag ggccgccccc ggggttccgg gcgaagccgg
      721 gcgcgccgcc cgattctgca ggaggcgaaa acgaatatcg ctgccggcgg gcgtggttac
      781 aatcaccacg tcccgaccgg cattttcagc gaggatttca aatttgcagg gagcctcgga
      841 cgaagcagcc gcgccgcccg cctgcgccga tccgtggccg gcggcaggcg tgaccaccag
      901 ctccttgagg ctgaccttct caaaccagct ctcgagtgcg gtcggcgatg cgccgctctc
      961 cagcgggacc cgggccgcgg acgcagggac ggacacgtgg ggagccggga tctccgacgg
     1021 ctgcggcaac ggcagcgaaa tgcgaatggc cgggtgcccc gggtctttcg gggaaagatc
     1081 gagcatcagc cggccctcgg ccgcgcgcgc gtcggtgatt atgtacgcac cggccgggag
     1141 cgtgtcgagc ggctgcgggg taagatcctg aatcagttgc agtgcggccg gggtgggaac
     1201 gaaagttccg gttccgatag atcccggcgg gttcgtcgcc gccggggagt attcgtccga
     1261 gacagcagcg gcctggatcg ccgcccgccc ctggaggacc gaccgggcct tgtcataaat
     1321 gtcaccgctc gtcgtctcca gcgcctcgaa atcccatccc ggcgaaacga ggaggtcgtc
     1381 caagagtgcg cgttcaccgt cgcgtgtcgc cgggacaagc ggacggcagg cggcggccag
     1441 agtatcgtcg gcgggccgag cggtctgacc ggggaccgaa gcggcctgcg tccccgatgg
     1501 catcgaggcg gttttcgcca acggaaggag cgatgtcacc cgagacgggc cgagggcggt
     1561 ctgcatgaga gcgccgaaca gcagttccgg cggagcgccc gcctctttcc cggccgtcgc
     1621 cccggagggt tcgactgtcg gcagtggcgc gactccgagc aggaggttca taatatcggg
     1681 tgacagggtc ggcatcgtta tttttccgca atcgtgatca tctgtttgga caggcgcgcc
     1741 gcccgctcgg gggggagcag ttggagcacc tcggaggcgt tcttggcttt catgcgcggc
     1801 aggatcgaca cgaccgtctc atcgtcgagg ttggtcatga gcttggccac ggcgtgcgag
     1861 tccatcccgt cgtagagctt ggcgagaccg gcggtgcggg ccgattcggc ctgttcaagc
     1921 acgagcatct ggcggctgac ctcgttctcg cggcggacga gatcgttctc cctggtcttc
     1981 agttgcgact cccattgggc gagccgtttc tgctcctcgg ccaggtggtc ggccgtcccg
     2041 ggtgcgccct cgatcgccgg tgccgtatca ccggcggccg cactctccgc cgggtcgtag
     2101 tcgagcacgt tcaggttctc ctgaatgagc gcgagcatgt cggagtcgtc ctcgccggcc
     2161 aggagactgt cgaggatctg gtcgtcggtc agttgcgccg cctgggcggc gcggttcccg
     2221 gcgagagtgc tgtcggtgtc gcccggtgc
//
LOCUS       DAOOKS010000323         2245 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_60287_length_2245_cov_3.918265, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000323 DAOOKS010000000
VERSION     DAOOKS010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60287_length_2245_cov_3.918265"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="PK112_05860"
     CDS             <1..339
                     /locus_tag="PK112_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HPC11607.1"
                     /translation="ILGFAAMLTLAAIVGKQACSLAIFDRRTNRLAIGLGMIPRGEVG
                     LIFAGIGLTLMLDGHAVVSSETYAAVVIMVIVTTLVTPPLLIWSMHRGGTTRTEAPAE
                     KSGAAGRPTP"
     gene            341..1204
                     /locus_tag="PK112_05865"
     CDS             341..1204
                     /locus_tag="PK112_05865"
                     /inference="COORDINATES: protein motif:HMM:NF037026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPC11608.1"
                     /translation="MEIAPLTIAGLEFRLREEPGIEREWFTARLRDYLAYEPGGCFAL
                     AEGGETVGMVTALCYQTTAWLGWLYVAAARRGAGLGERLMRQGMGYCIARGMRSIVLE
                     AVVEAAPLYRRIGFQRQFRTQHYRLEKTRRGAAPSPSVAVRRFAADDLVRIADLDRRM
                     FGQDRRRVLDLVRRNPLHRGWLAEKEGKVAGYLWATKAAANRQAGPWVIDPGTGDAAE
                     TAAALCDAAFAESDRPLILRCPLVTESRARLWDDLGAQAIDYHTERMYAGEEYAPEGE
                     GVLCLGCPGKG"
     gene            1349..1422
                     /locus_tag="PK112_05870"
     tRNA            1349..1422
                     /locus_tag="PK112_05870"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1383..1385,aa:Met,seq:cat)
     gene            1692..2117
                     /locus_tag="PK112_05875"
     CDS             1692..2117
                     /locus_tag="PK112_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11609.1"
                     /translation="MFVHNILAGRWHADYNIIKGNGHYRESRLSEETQMRDRTHRAFV
                     EVIRALSLPTICHDALLWSPDGDPVPPGPGRAGGGAPFGGIELFPAGTFGTDRPCAGA
                     AWYRAAGGRSLSFTASGLRSLALPPLFFLSIRFAQEVTS"
BASE COUNT          374 a          683 c          783 g          405 t
ORIGIN      
        1 attctagggt ttgccgcgat gctcacgctg gccgcgattg tcggtaaaca ggcttgctcg
       61 ctggccattt tcgaccgccg caccaaccgg ctcgcgatcg gactgggaat gatcccgcgc
      121 ggcgaggtcg gactgatctt cgccgggatc gggctcacgc tcatgctcga cgggcacgcg
      181 gtcgtgtcga gcgaaactta cgctgcggtc gtgatcatgg tcatcgtgac gaccctggtg
      241 acgccgcccc tcttgatctg gtcgatgcac cggggaggga ccacccggac cgaggcgccg
      301 gcggaaaagt ccggtgcggc cggccggccg acgccgtaga atggagatcg cgccgctgac
      361 aatcgcaggg ctggagttcc ggctgcggga agaaccggga atcgaacggg aatggttcac
      421 ggcgcgcctg cgggactatc tcgcctatga accgggaggg tgtttcgcgc tcgccgaagg
      481 gggcgagacg gtcgggatgg tgaccgcttt gtgctaccag acgaccgcct ggctggggtg
      541 gctctatgtg gcggcggcgc gccgcggcgc cggactcggg gaacgcctca tgcgccaggg
      601 aatggggtac tgcatcgcgc gcgggatgcg ctcgatcgtg ctcgaagcgg tggtggaggc
      661 ggcgccgctc taccggcgga tcgggtttca aagacagttt cgcacccagc actaccgcct
      721 cgaaaaaact cgccgcggcg cggcgccctc gccgagcgtg gccgtgcggc gcttcgcggc
      781 cgatgatctc gtccgcatcg ccgatctgga ccggcggatg ttcggacagg accgccgccg
      841 ggtgctcgac ctcgtgcggc gcaatcccct gcaccggggg tggctggcgg agaaagaggg
      901 gaaggtcgcg gggtacttgt gggccaccaa ggcggcggcc aataggcagg cgggaccctg
      961 ggtgatcgac ccggggacgg gggatgcggc ggaaacggcg gccgcgttgt gcgacgccgc
     1021 cttcgcagag agcgaccggc cgctaatcct gcgctgcccg ctcgtgaccg aatctcgggc
     1081 gcggctgtgg gacgatctcg gggcgcaggc gattgactat cacaccgagc ggatgtatgc
     1141 gggcgaggag tatgcgcccg agggagaagg tgtcctttgc ctgggctgtc cggggaaagg
     1201 ataggcggcg ggccacgcaa cttggacggg cgcaccgggg cggggcggcg cgcgttcggg
     1261 aggagacatc atttctcaag aaatcgacga gcgcacgaga atcggacttg acaacggggg
     1321 ttggcggggt atcttggggt ctgttgatcg cggggtggag cagtctggta gctcgtcggg
     1381 ctcataaccc gaaggtcgga ggttcaaatc ctccccccgc cattactcaa gttggatgct
     1441 cccccgataa tccccagaat ccgctccact cgcggcttct cctccagcat cactccgcgc
     1501 tgatcgttct cggttaccgc tagagcggtc aggaggaccc gcagtccggc gatatagcat
     1561 gcagcctcgt acactcgccg cgaatcggcg ggcggatgtc gcctcgatca gatcactgcg
     1621 ccgacatgag ctcgcattgg gactgcagat tgtcaccgaa catctatgcc cgatatgcaa
     1681 cgaaaatagt gatgtttgtc cacaacatac ttgcaggccg gtggcatgct gactataata
     1741 tcatcaaagg caatggtcat tatcgtgaga gtcggctctc cgaggaaacg cagatgcgtg
     1801 accgaacaca tcgggccttc gtcgaagtca ttcgggcgct gagcctgccg acgatatgtc
     1861 acgacgcgct gctttggtct cccgatggtg atcccgttcc gcccgggccc gggcgggcag
     1921 ggggcggcgc gccattcggc ggaattgagt tgtttcccgc cggaacattc gggaccgatc
     1981 gcccgtgcgc cggcgccgca tggtatcgcg ccgccggcgg ccgcagcctt tcgttcaccg
     2041 catccggctt gcgttcgctg gccttaccac cactgttttt tctctccatt cgctttgccc
     2101 aggaggtgac atcgtgatga ctcgcgcgag gaagcactat ctcttgttga tccctgcgat
     2161 cgctttcgca gcggccctcg gcgccggtgg ctgcggcacg aaagaagggg ttcctgccgc
     2221 cggcccgccg gaggtcaggg tggca
//
LOCUS       DAOOKS010000324         2245 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_60297_length_2245_cov_3.205479, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000324 DAOOKS010000000
VERSION     DAOOKS010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60297_length_2245_cov_3.205479"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..955)
                     /locus_tag="PK112_05880"
     CDS             complement(<1..955)
                     /locus_tag="PK112_05880"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPC11610.1"
                     /translation="MVKKVFLITFIVVIVGGVLFFAIDGSAKKEEGLKTVPVERGDII
                     DKALAVGRIVPEKEIAVKSKISGIVKRRYVDVGETVAVGDPLFDIAPDPTPLEFAESK
                     RQVELAQVTFDNAKREFDRTQSLKDKQLISNQEFDKARAAYEEAELRLKLAEERLSLI
                     QSGRTTVADLQVDNTIKAPISGTVLSILVEEGDPVVPLTSYQAGTELMTIARMDNLVF
                     KGDVDEIDVGKLHEDMGAEIEVGALPNSKVTGRLTKISPKASQKEGSTLFEVEIALGE
                     VSDTFLRAGYSANADIIITKKEGILTVPERLDNTGKERVPLD"
     gene            1168..>2245
                     /locus_tag="PK112_05885"
     CDS             1168..>2245
                     /locus_tag="PK112_05885"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPC11611.1"
                     /translation="MAMTQGYIVGRPAAAIARPPTAPPHLAAAAPADRRPQPQPATLR
                     AGRDSSLSMALPAFAARTTVFRWLRDYFLGLRLLVRSLPRFASLTVTYHSDLSFVDQV
                     APVLLLAKFFGVRTTVLYRSNKAETELDDYARAIIPFLRLSDRVVVSCAYLAEVFSRY
                     GLTAAVRADTVDAELFPAKQVTAVQPHIIMTRRLAPGNGLTGALKAFHLVKQKYPRAE
                     LTIVGDGPLRPWLEEFVRRERIYGVTFTGRVDHAEVARRMAAADIYLNNATIDALPTS
                     LLEALACGRCVVSTAVGEIPRVICDGRNGLLVAPNNFAGLADRLLELIETPALCARLS
                     RHAPASLAQWAASANAPDPATPDPR"
BASE COUNT          329 a          793 c          702 g          421 t
ORIGIN      
        1 cgtcgagcgg cacccgctcc ttgccggtat tgtccagccg ctccggcacc gtcagtatcc
       61 cctccttctt cgtaatgatg atgtccgcgt tggcgctgta cccggcgcgg aggaaggtgt
      121 cgctcacttc gcccagagcg atttccacct cgaacagcgt cgacccctct ttctgcgagg
      181 ccttggggga gatcttcgtg aggcggccgg tgactttgct gttgggcagg gccccgacct
      241 cgatttccgc ccccatgtct tcgtgcagct tgccgacgtc gatttcgtcg acgtccccct
      301 tgaacacgag gttgtccatg cgggcgatcg tcatcagctc ggtgccggcc tggtaggaag
      361 tgagcggaac gaccggatcg ccctcctcga cgagaatgga gagcacggtc cccgaaatgg
      421 gggccttgat cgtgttgtcg acctgcaggt cggcgaccgt ggtccgcccc gactggatga
      481 gggagaggcg ctcctcggcc agtttgagcc gaagttccgc ctcctcgtac gcggctcgcg
      541 ctttgtcgaa ctcctggttg gagatgagct gtttgtcctt gagcgactgc gtgcggtcga
      601 attcccgttt ggcgttgtcg aacgtcacct gcgcgagctc gacctggcgc ttggactccg
      661 cgaactccag cggcgtcggg tcgggggcga tgtcaaagag cgggtcgccg accgccacgg
      721 tttcgccgac gtcgacgtag cggcgtttga cgatgccgga gatcttggac ttgaccgcga
      781 tttctttctc cggcacgatc cgccctaccg ccagggcctt gtcaataatg tccccccgct
      841 ccaccggcac cgttttcagc ccctcctcct tcttggccga gccgtcgatg gcgaaaaaca
      901 gaacccctcc gacgatgacg acgatgaagg tgatgaggaa gaccttcttg accattgggc
      961 ctgactccct gtaacgtgag atttgcgtcg ctgttcaact tctacgtcag gcaatacggg
     1021 agggccggcg aattgtttcg ggcgccctcg ccctggcgcg gcccccgggg cccccgggtg
     1081 aacttcgtgc ccccccgcgc gtacaagaaa taccgggccg gctcccccgc ggtcccggtc
     1141 cttatctcag cggaggcagc agcagcgatg gcgatgacgc aaggatacat agtcggccgc
     1201 ccggccgcgg cgatagcccg cccgccgacc gcgcccccgc acctcgcggc ggccgccccc
     1261 gcggaccgcc gcccccagcc ccaaccggcg actctgcggg cgggccggga cagttcgctg
     1321 tcgatggcgc tcccggcttt tgccgcccgc accaccgtct tccgctggct ccgcgactac
     1381 ttcctgggac tgcgcctcct ggtccggtcg ctcccccgct tcgcctcgct gaccgtcact
     1441 taccactccg atctctcgtt cgtcgaccag gtcgcccccg tgctactgct ggcgaaattc
     1501 ttcggcgtcc ggaccaccgt tctctaccgc agcaacaagg cggaaaccga gctggacgac
     1561 tacgcgcggg cgattatccc cttcctgcgg ctgagcgatc gggtggtcgt gtcgtgcgcg
     1621 tatctggcgg aggtgttctc ccgctacggg ctgaccgccg cggtgcgggc ggatacggtc
     1681 gatgccgagt tgttccctgc caaacaggtc accgccgtgc agccgcacat catcatgacc
     1741 cgacggctgg cgcccggaaa cggtctgacc ggcgcgctga aggcgttcca cctggtcaaa
     1801 cagaaatacc cgcgggccga gctgacgatc gtgggggatg gtccgttgcg cccgtggctg
     1861 gaggagtttg tccgccgcga gcgcatctac ggcgtgacct tcaccggccg ggtcgaccat
     1921 gccgaggtcg cccgccgcat ggccgccgcc gatatctatc tcaacaacgc caccatcgac
     1981 gccctcccga cttcgctgct ggaagcgctg gcctgcgggc ggtgcgtcgt cagcaccgcg
     2041 gtcggggaga tcccgagagt gatctgcgat ggccgtaacg ggctgctcgt ggcgccgaac
     2101 aatttcgccg gcctcgccga ccggctgctc gaactgatcg aaacgcccgc cctgtgcgcc
     2161 cggctctcgc gccacgcgcc cgcgagcctg gcccaatggg cggcttcggc aaatgcgccg
     2221 gatcccgcca cgcccgatcc cagga
//
LOCUS       DAOOKS010000325         2225 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_61078_length_2225_cov_2.324885, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000325 DAOOKS010000000
VERSION     DAOOKS010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61078_length_2225_cov_2.324885"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="PK112_05890"
     CDS             <1..218
                     /locus_tag="PK112_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11612.1"
                     /translation="KQLAEVDGCDTACLDLSPERHQTARRVCPDDDSRIAVRLRRDRD
                     GRERRRQYQCQNPFQPSAPSSLVNGLL"
     gene            complement(327..1181)
                     /locus_tag="PK112_05895"
     CDS             complement(327..1181)
                     /locus_tag="PK112_05895"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HPC11613.1"
                     /translation="MSSVDVSLDHAVATPYLGLVEQVKYFACEYLHGLLFVACSMLFV
                     MAFPAALTKPAKVKAMVNRGVKRTLDIVGAIIGLTLSLPIFLIVPILIKLDSPGPIFY
                     TQTRIGVNRRRRDRRYHQRSEIDTEQRQRNRRRQDLKGKPFQVVKFRTMVANAEQLSG
                     PVWASKGDPRITRLGQFMRKTRLDEIPQLLNVLMGDMSLVGPRPERPTFVAELSSKID
                     NYCDRLQVKPGLTGLAQVECGYDTDLASVVKKVSYDLAYIENWSIWLEIKILLRTVVV
                     VVTGRGAN"
     gene            complement(1624..>2225)
                     /locus_tag="PK112_05900"
     CDS             complement(1624..>2225)
                     /locus_tag="PK112_05900"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HPC11614.1"
                     /translation="LNYGTIDGRDINGLATGDVTAYDWFGAVSYSYDLSPAVSLGLTG
                     KFVNQKLDDLSASSFAADIGGAYRFDKVTLAAVLANVGPDMSFENVSEHLPSSARVGA
                     AVALFDRQVLAAAEMEKPFYGDWVFRQGLEYGYRDQYFVRAGYNYYPGAEDRSFGSGM
                     SLGAGVQFSRLGIDYAYTVQEHYTSEDLHRFSLEFRFGD"
BASE COUNT          455 a          730 c          605 g          435 t
ORIGIN      
        1 ataagcagtt ggcggaagtg gacgggtgcg acaccgcttg cctggatctc tccccggagc
       61 gtcaccagac agcccggcgg gtctgtcctg acgatgactc ccggatcgct gtccgcctgc
      121 gccgcgaccg ggacgggcgc gagcgccgcc gccagtatca atgccagaat ccctttcagc
      181 cttccgctcc ttcttccctg gtgaacgggt tactctgatt atcgggaaaa tagtgcgtcg
      241 tttgagccgg cacaagatat aacacaaacg gccgtggaac acggccgttt gacaggtctc
      301 tcaggctcgg tggtcacagg gggcgcctaa ttagcacccc ggcccgtcac caccaccacc
      361 accgtacgta gcagaatctt gatttccagc cagatcgacc agttctcgat ataggccagg
      421 tcgtagctga ccttcttcac caccgaggcg aggtcggtgt cgtacccgca ttcgacctgc
      481 gccagtccgg tcaaccccgg ctttacctgc agccggtcgc agtagttgtc gattttcgag
      541 gagagttcgg ccacaaacgt gggacgctcc gggcgcggcc cgaccagcga catatccccc
      601 atgaggacgt tgaggagctg cgggatttca tccaaccgcg tcttgcgcat gaactgcccc
      661 aggcgcgtga tccggggatc tcccttgctc gcccacaccg ggccgctgag ctgctcggcg
      721 ttggcgacca tcgtccggaa cttgactacc tggaacggct tgcctttcag atcctggcgc
      781 cggcggttgc gctgccgctg ctcggtgtca atttccgagc gctggtgata gcgccggtcg
      841 cggcggcggc ggttgacccc gatccgcgtc tgagtgtaga aaatcggacc aggtgagtcc
      901 aacttgatga ggatcggcac aatcaggaag atcggcaggg agagcgtcag gccgattatg
      961 gcacccacta tgtccagcgt gcgcttcacg cctcgattga ccatcgcctt caccttagcc
     1021 ggcttcgtca acgccgccgg aaacgccatg acgaaaagca tcgagcaggc cacgaacaac
     1081 aggccgtgga ggtactcgca cgcgaaatat ttaacctgct caaccagacc aagatacggc
     1141 gtcgctaccg cgtggtccag agaaacgtcg acgctcgaca ccgatccgta cgcgaattcc
     1201 cggcgcgcta ctgctccggc gctctcattt gttatatgat ggtgattagg tatggaacta
     1261 ctcatattct tcgttagagc ccggcgtttt cgtacgccct gctgtttacc gcaacatggt
     1321 tagatctgct ctaagcaaaa ctattgccaa cttcctatac atcgccccgc gtcgcgccga
     1381 ttcaccgccg actatttgca atccattatt acccaatgag taacggccgt ttcgccgtaa
     1441 agaattctgc gatttcatta cccccgaacc gaccgtgccc ctgccgcatc gcgtcatgtg
     1501 ctgacagtat ccacatatta tttgcaattg gttgcactcc ccctgcagca gctccgccga
     1561 ggacaaaaaa agagccggcc ctctggccag ctccaactcg atcactccaa atgacttcag
     1621 ccgctagtcg ccgaaccgga actccaggga aaaccgatgc aaatcctccg aagtgtaatg
     1681 ctcctgaacg gtgtaggcgt aatcgatccc caagcgactg aattgcacgc ccgctccaag
     1741 actcatcccc gagccgaacg accgatcctc ggcccctggg tagtagttgt acccggcgcg
     1801 cacaaaatac tggtcccggt agccgtactc cagcccctga cggaagaccc agtctccgta
     1861 gaagggtttc tccatctcgg ccgccgccag cacctggcgg tcaaagagcg ccacggccgc
     1921 cccgacgcgc gccgacgacg gcaagtgttc gctgacattt tcaaacgaca tgtccggacc
     1981 gacattcgcc agcaccgccg ccagcgtcac cttgtcgaag cggtaggccc cgccgatgtc
     2041 cgcggcgaag ctcgaggcgc tcaagtcatc aagcttctgg ttgacgaact tccccgtcag
     2101 ccccagcgac acggcgggcg acaggtcgta ggagtacgag accgctccga accaatcata
     2161 ggcggtcacg tcccccgtgg ccaatccgtt gatgtcgcgg ccgtcgatcg tcccgtagtt
     2221 gagga
//
LOCUS       DAOOKS010000326         2222 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_61176_length_2222_cov_2.739732, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000326 DAOOKS010000000
VERSION     DAOOKS010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61176_length_2222_cov_2.739732"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1141
                     /locus_tag="PK112_05905"
     CDS             <1..1141
                     /locus_tag="PK112_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11615.1"
                     /translation="PEGGSFRVGGDVLAYDWIFMSVENAGAADTDLKAPANCLLRDRA
                     TNPRDNQVALVLPYSDSIFYSTCYFCLSTPSDRDRDTNNMDAGLWFCQYQLEQVNRDV
                     RIGLDSVTYQQKVYPQESIGVVIYTDYTIDPENPLETLQLNYSFDTLEIIQARVTIDS
                     VIISDPPETVMVANPYWQLGERIDTLFDNFTQGTEGFGMVDYSEFGLQYRGWVMAQAV
                     EAQNASVGTFTPPVWYRDNLFNVFRWPEGSGLISTGAFGSVEDFDLGNPHSASTRVPP
                     YPGEDFLVDLPHGAAGPLNLIAKGELRYYPGDVFISLEPWNYKDTTTNFPLILYTGFL
                     PFGAKGELYPDTTSDSPIQYFVMRNLTSPDADIGMPKVIVDIDRF"
     gene            1873..>2222
                     /locus_tag="PK112_05910"
     CDS             1873..>2222
                     /locus_tag="PK112_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11616.1"
                     /translation="MENVMAPAAPVEKVHHPGLGFKGLWQVFVAPSQLFAKVKEDPKV
                     LVPYIVLGLAILGLMLLLADYILQAQMQSEQFQKSLEQRGVTREQLTGQVLTFMKLST
                     VAAGTLGMLLVPLL"
BASE COUNT          405 a          702 c          682 g          433 t
ORIGIN      
        1 ccccgagggc gggtccttcc gggtgggcgg cgatgttctg gcctacgatt ggatctttat
       61 gtcggtcgag aatgccggag ccgcggacac cgatcttaag gcgccggcca attgcctgct
      121 gcgcgatcgg gctaccaatc cgcgcgacaa ccaggtggcg ctcgtgctcc cctacagcga
      181 ctcgatcttt tactctacct gctacttctg cctgtccacc ccctccgacc gggaccgcga
      241 cacgaacaac atggacgccg gcctatggtt ctgccaatat cagctcgagc aggtgaaccg
      301 cgacgtgcgg atcgggctgg acagcgtgac ctaccagcag aaggtgtatc cgcaggagtc
      361 cattggggtc gtcatttaca ccgactacac gatcgacccg gagaacccgc tggagacgct
      421 gcagttgaac tactccttcg acacccttga gatcattcag gcgcgggtca cgatcgacag
      481 cgtgatcatc tccgatccgc ccgagacggt catggtcgcc aatccctatt ggcagctggg
      541 ggagcgcatc gacaccctct tcgacaactt tacccagggc accgagggat tcgggatggt
      601 cgactacagc gagttcggcc tgcagtatcg ggggtgggtt atggcgcagg ctgtggaggc
      661 acagaacgcg agcgtgggta cgtttacgcc gccggtctgg taccgcgaca acctgttcaa
      721 tgtcttccgc tggcccgagg ggagcggtct gatcagcacc ggcgctttcg gttcggtcga
      781 agacttcgat ttgggaaacc cgcactcggc cagtacgcgc gtcccgccat acccgggcga
      841 ggattttctg gtggatctcc cccacggcgc ggcgggcccg ctgaatctca tcgccaaagg
      901 ggaactgcgg tactatcctg gggatgtgtt catctcgctg gagccatgga actacaagga
      961 tactaccacc aatttcccgc tcatcctcta caccgggttc ctgccgttcg gcgccaaggg
     1021 tgagctgtat cccgacacca ccagcgacag ccccatccag tactttgtca tgcggaacct
     1081 gacctcgccg gacgcggaca tcgggatgcc caaagtgatc gtggatatcg accgattcta
     1141 gccgatcgct tctccgagca agacttgaca gaggccggcg cgcgccggcc tctttgcttt
     1201 ttccggccgc ccggcgccag tccggcacat ctcggagtgg tctcgccacc gcctactcaa
     1261 gtgattctgg acggcgttgc ccggcggggt caagttacag cagggcaagg tcttacagcc
     1321 gacacgatgc tttggctcac agggcccccc tggcgcccgc tcccaccgcg gctcatgatc
     1381 tgccgcatta tcgcgccacc ggcagcggcg ccgggtgctc tgcccgcctc gaacgcggaa
     1441 gccgggtcca gaccaggcgt cgaatgcagc gacgacattg aggaggacct ccagccatca
     1501 gctcctttga tcgcgcgcgg caaccgggat tctcctccgc cgcagcggta ccgccgcctg
     1561 tccggtgcgc ggccgctgca gtcgccatag ttttctgaaa cgccgactga gggcgaccga
     1621 cgacatgccc gctcatgcgc cggccgatca caccgcgggc aggcgcggtg gcgcagtcgt
     1681 cgtccattaa tccaaatcta gcttgatccg gtcgtattgt ggagctatcc tgtgggacgg
     1741 ctcccggtca attttgacgt gccgcgttga ataagccgcc gaatcggggg taccaattgc
     1801 agccgacggc tggggagagt gaaacactgg ccggacggat gcgtacaact gttccatgag
     1861 gaaaaactga agatggagaa cgtgatggct cccgcggcgc ccgtggagaa agtgcatcac
     1921 cccgggctgg ggtttaaagg gctgtggcag gtctttgtgg ctccctctca attgttcgcg
     1981 aaggtcaaag aggatcccaa ggtgcttgtg ccgtacattg tcctcggcct ggccatcctg
     2041 gggctgatgc tgctgttggc ggactacatc ctccaggccc agatgcagag cgagcagttc
     2101 cagaaatcgc tcgaacagcg cggcgtcacc cgcgagcagt tgaccgggca ggtgctcacg
     2161 tttatgaagt tgtcgacggt ggccgccggc acgctcggga tgttgctggt gcctctgctc
     2221 at
//
LOCUS       DAOOKS010000327         2217 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_61333_length_2217_cov_4.488437, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000327 DAOOKS010000000
VERSION     DAOOKS010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61333_length_2217_cov_4.488437"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1383
                     /gene="menD"
                     /locus_tag="PK112_05915"
     CDS             <1..1383
                     /gene="menD"
                     /locus_tag="PK112_05915"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HPC11617.1"
                     /translation="LYADYPRWFFDLPCPSRETPPEFVLTTIDQAVYRAQHGPAGPVH
                     LNWMFREPLAPVGAEEDFGDYLRSVGCWRTGESPWTIYRSPVKVCEPARMEAATGLLN
                     RARRGVIVVGRLRREAERVAIAKVAEALGWPVIADIGSGLRLGAAGHVIAPAEYMLAA
                     ESFADALAPDTVLHFGPIVTSKRVAQWLKRSQPAAYIHAAAAPERHDPHHQVTHRFEA
                     DIDMFCSFLLPFLREKAKETGLAAWRAGADKAEAAIARVLAEDATLSEPLTARLVAEN
                     ISAAGALFVASSMPIRDLDALAAGDGPAAPVAANRGASGIDGTVASAAGFGLGWERPV
                     TLLTGDLALLHDLNSLALVRRLAQPMTIVVLNNNGGGIFHFLPIAERADLFEPYWGTP
                     HGITFKQAAQMFGLSYAHPTSAEEFARIYREAQAGPGGGTLIEVRTDRRRNRQLHARL
                     TEAVVRALEG"
     gene            1388..2185
                     /gene="menH"
                     /locus_tag="PK112_05920"
     CDS             1388..2185
                     /gene="menH"
                     /locus_tag="PK112_05920"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR03695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HPC11618.1"
                     /translation="MAGDFRFHLETAGSPRNPAVVALHGFMGTGADWSTVMAALAGER
                     YGLAPDLPGHGKTRGPEGAGSWRMEQVAAALALELARRGIAQTDLIGYSMGGRLGLHL
                     ALHCRARVRRLVLESASPGLSDGDERIARRRQDDTLARRLETEPLERFLRDWYDQPMF
                     AGLKRVPEEFAGMLERRRAQDGGALARALREMGTGAQEPQWDRLADAPPTLLIVGAED
                     AKFREIAARMAERMPGARVATVAGAGHAVHVEQPARFVELVQEFLSG"
BASE COUNT          332 a          706 c          846 g          333 t
ORIGIN      
        1 ctctacgccg attatccccg gtggtttttc gatctcccct gccccagccg ggagacaccg
       61 ccggagtttg tgctcacgac gatcgaccag gcggtctacc gggcgcagca cggaccggcc
      121 ggtccggtgc acctcaactg gatgttccgg gaaccgctcg ccccggtcgg cgcggaggaa
      181 gatttcggcg actacctgcg gtcggtcggg tgctggcgaa cgggcgagag cccgtggaca
      241 atctatcgct cgccggtgaa agtgtgcgaa ccggcgcgga tggaagcggc gaccgggctg
      301 ctgaaccggg cgcgccgcgg tgtgatcgtg gtcgggcgtc ttcggcgcga ggccgagcgg
      361 gtcgcgatcg cgaaagtggc cgaggcgctc gggtggccgg tcatagccga tatcgggtcg
      421 ggcctgcggc tcggggcggc gggacacgtg atcgcaccgg ccgagtacat gctcgccgcg
      481 gaatcgtttg ccgacgctct cgcgccggac acggtgctcc acttcggtcc gatcgtaacc
      541 tcgaagcggg tcgcccaatg gctgaaacgg tcgcaaccgg cggcctatat ccacgcagcc
      601 gccgcccccg aacgccacga cccgcaccat caggtcaccc accggttcga agcggacatc
      661 gacatgttct gcagttttct cctgccgttc ctgagggaga aagccaaaga gaccggcctt
      721 gcggcctggc gggcgggtgc ggacaaggcg gaagcggcga tcgcccgagt gcttgcggag
      781 gatgcgacgc tgtcggagcc gctgacggcg cggctggtgg cggagaatat ctcagccgcg
      841 ggcgcgctct ttgtcgcctc gagcatgccg atccgggacc tggatgccct ggccgccggc
      901 gacggcccgg cggcgccggt tgcagccaac cggggcgcga gtggaatcga cggcacggtc
      961 gcctcggcgg ccgggttcgg gctcgggtgg gagcggccgg tgaccctgct gaccggggat
     1021 ctggcgctgt tgcacgacct caactccctg gcgctcgtgc ggcgcttggc gcagcccatg
     1081 actatcgtcg tgctgaacaa caacggcggc ggaatattcc atttcctccc gatcgccgag
     1141 cgcgccgatt tgtttgagcc gtactgggga acgccccacg ggatcacgtt caagcaagcg
     1201 gcgcagatgt tcgggctttc gtacgcccat cccacgagcg ccgaagagtt cgcgcgcatc
     1261 taccgggagg cccaggccgg gccgggcggg gggacgctca tcgaggtgcg aacagaccgg
     1321 cggcgcaacc ggcaactgca cgcccggctc accgaggcgg tggtccgcgc gctcgagggg
     1381 taagcgcatg gcgggggatt tccggtttca cctcgagacg gcggggtcgc cgcgcaatcc
     1441 ggcggtggtg gcgctccacg gtttcatggg aacgggcgcg gactggagca ccgtcatggc
     1501 ggccctggcc ggagaacggt atggcctcgc tcccgatctc ccggggcacg gaaagacccg
     1561 gggtccggag ggcgcgggat cgtggcgcat ggagcaggtc gccgccgcgc tcgccctcga
     1621 gctggcgcgc cgggggatcg cgcagacgga tctgatcggc tactcgatgg gggggcggtt
     1681 ggggctgcac ctcgcgctgc actgccgcgc gcgcgtgcgg cggctcgtgc tcgagtcggc
     1741 ctcgccggga ctgagcgacg gagacgagcg gatcgcgcgg cggcggcagg acgatacact
     1801 cgcgcggcgg ctggagaccg agcccctcga gcgctttctc cgcgactggt atgatcagcc
     1861 gatgttcgcg ggacttaagc gggttccgga ggaattcgcc gggatgttgg aacgccgccg
     1921 cgcccaggac ggcggagctc tggcccgggc gctgcgtgag atggggaccg gggcgcagga
     1981 accgcagtgg gaccgcctgg ccgacgcgcc gccgacgctg ttgattgtcg gcgccgaaga
     2041 tgctaaattc cgggagatcg cggcgcggat ggccgagcgg atgccggggg cgcgggtcgc
     2101 gacggtggcg ggggcggggc acgcggtcca cgtcgaacaa ccggcccggt ttgtcgagct
     2161 ggtacaggag ttcctgagcg gatagaaagg gtgccatggc gaggatcatc tggacag
//
LOCUS       DAOOKS010000328         2213 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_61526_length_2213_cov_3.822057, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000328 DAOOKS010000000
VERSION     DAOOKS010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61526_length_2213_cov_3.822057"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="PK112_05925"
     CDS             complement(<1..249)
                     /locus_tag="PK112_05925"
                     /inference="COORDINATES: protein motif:HMM:NF015427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HPC11619.1"
                     /translation="MLDLVAKLENRLAAIDREMAEPGAASDQRRMIELGRERRRVEDV
                     LAIGRDYRRLRAGIEEAQAVIRADEDDELMALAREELAA"
     gene            complement(333..1274)
                     /locus_tag="PK112_05930"
     CDS             complement(333..1274)
                     /locus_tag="PK112_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HPC11620.1"
                     /translation="MIEGVMMRSADRIATAVRKPNGEILVKCEPYLSLTKRHRVLGAP
                     IIRGTVSFAEMLVVGIRTLNFSADVAMREIEAAEAEKNGAAAAPGKDKKSNALVLELS
                     VAAAFALGIGLFFFLPLALASLVGIKKDAVGFNLLAGGIRLALFVGYVWMISFLKDFK
                     RIFQYHGAEHKSIYAYEMGDDLTPGRAALYTRFHPRCGTSFILIVALTAILIYAVSDS
                     LFAVIAGHPPSLATRFAVHLALLPLVAGGSYELLKLSGRTRDHKVTRVLIQPGLWLQA
                     ITTREPSFDQLEVAIVALETALGRTESKVAATRIPVS"
     gene            complement(1411..1626)
                     /gene="rpmE"
                     /locus_tag="PK112_05935"
     CDS             complement(1411..1626)
                     /gene="rpmE"
                     /locus_tag="PK112_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020881527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HPC11621.1"
                     /translation="MKAEGHPKTFDVTITCSCGATYPNRSTKKNISVEICSNCHPFFT
                     GKQRLIDTAGRVERFIQKYKKYQKDSK"
     gene            complement(1812..2084)
                     /locus_tag="PK112_05940"
     CDS             complement(1812..2084)
                     /locus_tag="PK112_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11622.1"
                     /translation="MLGRLVRRGGRLWMIDNNPPAEGRSLPAAGVDAHGNNFRRRFLD
                     NGEPFVILKNYFQRDELVSLCAPYGRVDRLVYGTYYWSILLSDMNL"
BASE COUNT          390 a          761 c          675 g          387 t
ORIGIN      
        1 ggcggcgagc tcctcgcgcg ccagcgccat cagctcgtcg tcctcatcgg cgcggatcac
       61 cgcctgggcc tcctcgatcc cggcgcggag gcggcggtag tcccgcccga tcgcgaggac
      121 gtcctcgacc cggcggcgct cccgtcccag ttcgatcatc cggcgctgat ccgatgccgc
      181 tcccggctcg gccatctcgc gatcgatggc ggccaggcgg ttctccaatt tggccacaag
      241 atcaagcaca gctcatcctc gcgttactcc acattctcgg aaacaagggt acggggaatt
      301 gtccgccggc gcaaggcgga atgtgcgggc gcctacgaca ccggaatccg cgtcgccgct
      361 actttcgatt ccgtccgccc gagcgccgtc tcgagcgcca cgatcgccac ctcgagctgg
      421 tcgaacgagg gctcccgggt ggtgatcgcc tgcagccaga gccccggctg gatcagcacc
      481 cgggtgacct tgtggtcgcg cgtccggccc gacagcttga gcagctcgta ggatcccccg
      541 gccaccagcg gcagcagcgc gaggtgcacc gcaaaccggg tcgccagcga gggcgggtgg
      601 ccggcgatca ccgcgaatag agagtcggac accgcataga tcaggatcgc cgtgagcgcc
      661 acgatgagga taaacgaggt gccgcagcgg gggtggaagc gcgtgtagag cgccgcccgc
      721 ccgggcgtga ggtcatcccc catctcgtag gcgtaaatcg atttgtgctc cgccccgtgg
      781 tactggaaaa tgcgcttgaa atccttcagg aacgagatca tccagacata gccgacaaac
      841 agcgccagcc ggattccccc ggccaggaga ttgaacccga ccgcatcttt cttgatcccg
      901 accagcgagg ccagcgcgag cgggagaaag aaaaacaggc cgatgccgag cgcgaaggcg
      961 gccgccaccg acagctctaa caccagcgcg ttcgactttt tatccttccc gggcgccgcc
     1021 gcggcgccgt tcttttccgc ctcggccgcc tcgatttccc gcatggccac gtccgccgag
     1081 aaattcagcg tcctgatgcc gaccacgagc atctccgcaa acgagaccgt tccgcggatg
     1141 atcggcgccc cgagcaccct atgccgcttg gtgagagaga ggtagggctc gcacttgacg
     1201 agaatctcgc cgttgggttt tcgcacggcg gtggcgatgc gatcggccga gcgcatcatt
     1261 acgccctcga tcacggcctg cccgcctaca ttcaagtccg gcatggctct ggttggctcc
     1321 cgtcgtggcc agggattcgc gcgcactgcc gccgcccgcc tgtccgcccc gccggcggtc
     1381 tcgggcggcc ccgcgcggcc cgctcctgcg ttacttcgaa tccttctggt acttcttgta
     1441 cttctggatg aagcgctcga cccggccggc cgtgtcaatc aggcgctgct tcccagtgaa
     1501 aaacggatgg cagttcgagc agatttcgac actgatattt ttcttggtcg accggttcgg
     1561 ataggtcgcc ccgcacgaac aggtgatcgt cacgtcgaat gtcttcgggt gtccttcagc
     1621 tttcacaact cgttcctcaa cgttggcttt gggttcggcc ctccggggtc gatcgcccga
     1681 cgcccggagc ctcttaatat atacgcatcc ccctgcctgg caagatgaat cttcctgccg
     1741 gcgcgctcgc cgcgcccgca cgtacaacct cgcgcgatcg cccgcggttc ccgcgcggcc
     1801 cgccaggcga atcataagtt catgtcggat aataagattg accagtagta ggtgccgtag
     1861 accaagcggt ccacgcgccc gtagggcgcg cacagcgaaa ccagctcgtc ccgctgaaag
     1921 tagttcttca agatcacgaa cggctcaccg ttgtccagaa accgccggcg gaaattgttg
     1981 ccgtgggcat caaccccggc cgccggcagc gaccgtccct cggccggcgg attgttatcg
     2041 atcatccaga ggcgcccgcc ccttctcacc agccgtccga gcatcggaaa aaatgtcggg
     2101 acgtctctct cggctggtgc gacagccaga acgccaggac caccagatcg aacgccccct
     2161 ctgcgaacgg ggggtgttca aggtcggcga tgagaaagtg cgggggtcgg cgg
//
LOCUS       DAOOKS010000329         2207 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_61785_length_2207_cov_3.300651, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000329 DAOOKS010000000
VERSION     DAOOKS010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61785_length_2207_cov_3.300651"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="PK112_05945"
     CDS             complement(<1..486)
                     /locus_tag="PK112_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11623.1"
                     /translation="MAWWQAIDKLDRRWTYLLVAITVIIPFILPAKFPVSITPEAKQL
                     YNYVEELPNGSVVMLVFDYYPSTIAECEPMTRTALHHLFRKDCKVVTLSNIPLGGPTM
                     AESVTREMARLYGKVYGVDFVNLGYKADYVAVLRGMGQSIKGIFPADNSGTPLDSLPL
                     MR"
     gene            complement(491..1150)
                     /locus_tag="PK112_05950"
     CDS             complement(491..1150)
                     /locus_tag="PK112_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11624.1"
                     /translation="MRTTFPIFVAFAVGLLYVVSFFFAESSPIGRVSETLLVWVSIIG
                     GFTLLLGVVSIVRVNWTAVARRRPGWIYRLCTLVSVFVMALPALLPASWSPLFGRAEG
                     SVYDWLFLYIDSPMMATMFAMLAFYIASAAYRAFRARNIEATILLLTAVVVMLWRVPM
                     GEAFLNLLPGDIPGWLNTYVMRGVNVAVQRGILIGAALGAASMSLRIILGIERTYMGK
                     G"
     gene            complement(1172..2047)
                     /locus_tag="PK112_05955"
     CDS             complement(1172..2047)
                     /locus_tag="PK112_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11625.1"
                     /translation="MRLQVPLRGAALIGAAVLADWLPAAAQESAASTSVFRADRINAL
                     IFVVLFSTAILLYTRWARQGKPLFLRKIAGLDALEEAVGRATEMGKPVLFIPGILELD
                     DIQTIASISILGRVAKITAQYETPLYVPVRYPLVLAAGQEVVEQAYREAGRPDAYDRD
                     MVRYVAGEQFAFTANVNGYMVREHPATNIYMGGFFAESLILAETGNAAGSIQIAGTAE
                     ASQLPFFVAACDYTLMGEELYAASAYLSHEPVMLGGLKGQDFVKLIIIIVIVVGAVLI
                     ALGFGDTFVGWFDSV"
BASE COUNT          439 a          760 c          688 g          320 t
ORIGIN      
        1 ccgcatgagc ggcagcgaat cgagcggcgt cccgctgttg tcggcgggga atatcccctt
       61 gatcgactgg cccatgccgc gcagaacggc gacgtagtcg gccttgtagc ccaggttgac
      121 gaaatcgacg ccgtacactt tcccgtacag ccgggccatc tcccgcgtca ccgactccgc
      181 catggtcggc ccgcccagtg ggatgttgct cagggtgacc accttgcagt ccttgcggaa
      241 cagatggtgc agagccgtcc gcgtcatcgg ctcgcactcc gcaatggtcg aggggtagta
      301 gtcgaacacc agcatcacta cgctgccgtt gggcagctcc tcgacataat tgtacagctg
      361 cttggcctcg ggcgtgatgc tcaccgggaa cttggccgga aggatgaacg ggatgatgac
      421 ggtgatggcc accaggaggt aggtccagcg gcggtccagt ttgtcgattg cctgccacca
      481 cgccatcggc tcagcccttc cccatgtagg tgcgttcgat gccgaggatg atgcgcagcg
      541 acatggaggc cgccccgagc gccgcgccga tgagaatgcc gcgctggacg gccacgttca
      601 ccccgcgcat gacgtacgtg ttgagccatc cggggatgtc gccgggcagc agattcaaaa
      661 acgcctcccc catcggcacc cgccagagca tcaccaccac cgccgtcagc agaaggatcg
      721 tggcctcgat gttgcgggcg cgaaacgccc ggtaggcggc cgaggcgatg tagaaggcca
      781 gcattgcgaa catcgtcgcc atcatcggcg agtcgatata gagaaacagc cagtcgtaga
      841 ccgagccctc ggcccgcccg aacagcggcg accaggaggc cggcaagagc gccggcaaag
      901 ccatgacaaa caccgacacc agcgtgcaca gacgatagat ccaccccggg cgccggcggg
      961 ccaccgccgt ccagttcacc cgcacgatcg acaccacccc cagcagcagc gtgaatccgc
     1021 cgatgatcga cacccacacg aggagcgtct ccgacacccg cccgatcggc gatgactcgg
     1081 cgaagaagaa cgacacgaca tagagcaggc cgaccgcgaa tgccacgaag atggggaaag
     1141 tcgttctcat aggtgaccgc ggcgccgagg cctacacgga atcgaaccac ccgacaaatg
     1201 tgtcgccgaa tccgagcgcg atcagcaccg cgccgacgac gatcacgatg atgatgatca
     1261 gcttcacaaa atcctgcccc ttcaggccgc cgagcatgac cggctcgtgc gacaggtacg
     1321 ccgaggccgc gtagagctcc tcccccatca gcgtgtagtc gcacgcggcc acgaagaaag
     1381 gcagctgcga ggcctccgcc gtgcccgcaa tctggatcga cccggccgcg ttgccggtct
     1441 ccgcgaggat gagcgattcg gcgaaaaacc cccccatgta gatgttggtc gccgggtgct
     1501 cccggaccat gtacccgttc acgttggcgg tgaaggcgaa ctgctcgccc gccacgtacc
     1561 gcaccatgtc ccggtcgtag gcgtcgggac gccccgcttc ccggtacgcc tgctccacca
     1621 cctcctgccc ggccgccagc accagcgggt agcgaacggg cacgtagagc ggcgtttcgt
     1681 actgcgccgt gatcttggcc acccggccga ggatagagat gctcgcgatc gtctggatgt
     1741 cgtcaagctc aaggatcccc gggatgaaca gtaccggctt gcccatctcg gtcgcccggc
     1801 cgaccgcctc ctccagcgcg tcgagaccgg cgatcttccg aagaaacagc ggttttccct
     1861 gccgcgccca gcgtgtgtag aggaggatgg cggtggagaa aagtacgacg aagatcagcg
     1921 cgttgatccg gtcggcgcga aacacggagg tggaggcggc gctctcctgg gccgccgccg
     1981 gcagccaatc ggcaagaacg gccgcgccga tcagcgccgc cccgcgcagg ggaacctgta
     2041 accgcattcg cctcacgccg gaatataccg gttcaccggg gtttgtcaac gagaacgctt
     2101 cggaccggga ccggggaagg ccctggaaac aaaagctgcg cttggcccgg gaccgcgccc
     2161 cccgcgcccg gcgccgccgc cccgacatcc cgcgtgacaa cccccga
//
LOCUS       DAOOKS010000330         2204 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_61896_length_2204_cov_3.267101, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000330 DAOOKS010000000
VERSION     DAOOKS010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61896_length_2204_cov_3.267101"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(158..853)
                     /locus_tag="PK112_05960"
     CDS             complement(158..853)
                     /locus_tag="PK112_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11626.1"
                     /translation="MKFALGLEQTEPGKWIAWVVDLPGCFARASARDEVVRCAPRAIA
                     DYFNWRDGYERVSSEAIEQMGIEVVEEFRNYETLDGYWVNAFFAWDRRPLLAGEIDDV
                     RWLLSCMRSDLISAVRRVPAAALDQPIAGERFGTIAGILTHAATAERWYPDKLGLAPQ
                     KETLGTDPFAMLESVRRHLLMHLPRFANDATVSEGHGEFWSARKVARRALWHERVHTW
                     HIQRLTAQLVNQR"
     gene            1470..>2204
                     /locus_tag="PK112_05965"
     CDS             1470..>2204
                     /locus_tag="PK112_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11627.1"
                     /translation="MLFVLTLICSAGAWAQAEAATIATDSVALCFQEALFDTIAVDLP
                     NPGNPGVAMALVSGPGALTYSVSDKLYGYYEYAPSGDTAFEIVYRVWYAPTDSADFRH
                     RYIVWVDEPPTLGDQYNAFKVCWPGPHYLKVYAWDPESRPLAWEIVSGPGTIDSQGLI
                     TYTPDTAGVYVFEVAASDDCNRAAATVYDTVRFNGAPRIVLADSIFSLCAEGEICFDV
                     VFSDPDGDAVWFNQVGGPGSTTVVNDS"
BASE COUNT          393 a          665 c          727 g          419 t
ORIGIN      
        1 cgactgattg gtaacgcctt acgtgattct cctgcccctc cgcggggcga ggtcgggcaa
       61 gtggcgtgga cggctcttgg ccaccgccgg ggcggacctg gtgggcgggg gatgtcacgc
      121 tgcggcaaag cggtctgccc ggcggcgccg ggctcgttta ccgctggttc acaagttgcg
      181 ccgtcaggcg ctggatgtgc caggtatgga cccgctcgtg ccagagagcc cggcgggcga
      241 ctttgcgggc cgaccaaaac tccccgtgcc cctcgctcac cgtcgcatca ttggcgaagc
      301 ggggaaggtg catgagcagg tggcggcgga ccgattccag catcgcgaag ggatcggttc
      361 cgagagtctc tttctgcggc gccagaccga gcttgtccgg ataccagcgc tcggccgtgg
      421 ccgcgtgcgt gaggatcccg gcgatcgtgc cgaagcgctc ccccgcgatg ggctggtcga
      481 gggccgcggc cggtacccgg cgcacggcgg aaatcaggtc cgaccgcatg cagctcaaca
      541 gccaccgcac gtcgtcgatc tcacccgcca ggaggggacg gcggtcccag gcgaagaagg
      601 cattcaccca gtacccgtcg agcgtctcgt agttcctgaa ttcctcgacc acctcgatac
      661 ccatctgctc gatcgcctcg ctgctgacgc gctcataccc gtcccgccag ttgaagtaat
      721 cggcgatagc ccgcggggcg caccgcacga cttcgtcacg cgccgaggcc cgggcaaaac
      781 atccgggcag gtcaaccacc caggcaatcc atttgccggg ttcggtctgc tccagaccca
      841 gagcgaattt catgattccg ctgcaccgca gttttcaggt gaacctcaga ccaaggtaat
      901 gcggcagtcg acggatgtaa aggacagacc gccgtgtcct ccgggtttgc ggccggaggc
      961 ggcgcaccga ctgctccgag gcggggcggg ttgaaatctg actgtcgacc gatgatcggc
     1021 gcggggttca gatgcgccgg taggcaagga aaagcacaat ctgtcacagc ggcttcacaa
     1081 ttagggcggg gcggttgcag gagaggggcc ggggccggcg taagttcatt gagttcaggc
     1141 cgttaatctg ttggcccgat tcttgcgtca ctgttcctgc ggagtgaaat gaccttgact
     1201 tttagggacc ggaaagtata tttgtatggc caaagagacc cgcgcaatta ctgcactggc
     1261 gcatcgagtt tatacgtcca cgattggtgt ggggttaccg gcgctaaatg ttgcgttgac
     1321 aggtttccta tgccccgtcg gcgcatggaa atatggcact actggatcga tttgacactg
     1381 attccaggca gctgtccggt ggggactcga gcagccgggc agttcgaccg agaggaaaga
     1441 tgaaacactc agggattccg cgaattccaa tgctgtttgt cctcaccctt atctgcagcg
     1501 ccggcgcctg ggctcaggcg gaggcggcaa cgatcgccac cgactcggtg gccctctgtt
     1561 tccaggaggc gctcttcgat acgattgcgg tggacctgcc gaacccgggc aatccgggcg
     1621 tcgccatggc gctcgtcagc gggccggggg cgttgacata cagcgtgtcc gacaagctct
     1681 acggttacta tgaatacgcg ccgtccggcg acaccgcctt cgagatcgtc taccgggtgt
     1741 ggtatgcccc gacggactcg gcggatttcc ggcaccgcta cattgtgtgg gtcgatgagc
     1801 cgcccactct gggcgaccag tacaacgcgt tcaaggtctg ctggcccggt ccgcactact
     1861 tgaaagtgta cgcgtgggat cccgagtcga ggccgctcgc ctgggaaatt gtgtcgggac
     1921 cggggacaat cgacagtcag gggcttatca cctacacgcc cgacacggcg ggagtgtacg
     1981 tttttgaggt cgcggcgagc gacgactgca atagggcggc ggccacggtc tatgacaccg
     2041 tccgcttcaa cggcgcgccg cgaattgtcc tcgccgacag cattttcagc ctctgcgccg
     2101 agggggagat ctgtttcgac gtggtgtttt cggatccgga tggagacgcc gtctggttca
     2161 accaggtggg cgggcccggg tcaaccacgg tcgtgaatga ttcg
//
LOCUS       DAOOKS010000331         2203 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_61954_length_2203_cov_2.715084, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000331 DAOOKS010000000
VERSION     DAOOKS010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61954_length_2203_cov_2.715084"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="PK112_05970"
     CDS             complement(<1..270)
                     /locus_tag="PK112_05970"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HPC11628.1"
                     /translation="MTHEAVSTHVETPAATIDTRIRQIVSNIRNLPTPPVVFHQIQKV
                     ISDPNASAAQVAAILGEDPAMSVKVLKLTNSVFYGLAREVESVKQA"
     gene            complement(339..665)
                     /locus_tag="PK112_05975"
     CDS             complement(339..665)
                     /locus_tag="PK112_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11629.1"
                     /translation="MNIGRVRTVRPSLDTARTPGDETTVRRKGRNNAAPEEGGGARKA
                     GTPGDQLVISEDARARLAALADAARAEEILTPPPEDAEGLRMEKMRLASDRMRSGYYD
                     RPEIKR"
     gene            complement(809..1261)
                     /locus_tag="PK112_05980"
     CDS             complement(809..1261)
                     /locus_tag="PK112_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11630.1"
                     /translation="MDYDVLKQLERELLAVLKEEYTFYQSLYLTLDKQRDAVRFNRDD
                     HLLDLLAEVERYRQRIARSELTIRQLQEKHPKAFRLAAALPEVKRTINSIRTLVKKSV
                     DVVSECDEYLQGRVNRIKEELGELRNSQQILQYLSEPDPSPQFVDGKK"
     gene            complement(1271..1705)
                     /gene="fliS"
                     /locus_tag="PK112_05985"
     CDS             complement(1271..1705)
                     /gene="fliS"
                     /locus_tag="PK112_05985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HPC11631.1"
                     /translation="MASSKVAAYRREEMAGKSPLELVIQVYDGAISAFRAAADRYRSD
                     DAAGGREQLERAQTFVTHLYTTLDAERGGDISAQLGRLYAYVINRISVAAATGDPGVI
                     DDNIAILNNLREGWIGLRAQSADPAPSGPAPAAAPGGFVGSA"
     gene            complement(1729..>2203)
                     /gene="fliD"
                     /locus_tag="PK112_05990"
     CDS             complement(1729..>2203)
                     /gene="fliD"
                     /locus_tag="PK112_05990"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HPC11632.1"
                     /translation="LGLAAGESVAGQDVAGTINGETAEGKGQLLTGKTGNATTDGLVL
                     RVTLDETQVISGAEGQVTVTKGVAARLKDVVESLTKAEEGTFDRRIKSYQSQVDTIAK
                     RVAEIDERLKLRRESLLQKYYLMEQTLGEMQSIGNYLESQLAGINTNWGFRTSKQ"
BASE COUNT          274 a          771 c          679 g          479 t
ORIGIN      
        1 cgcctgtttg accgactcca cctcgcgcgc cagcccgtag aacaccgagt tggtcagctt
       61 gagcactttg accgacatcg ccgggtcttc cccgagaatg gccgccacct gggccgccga
      121 ggcgttgggg tcgctgatga ctttctgaat ctggtggaag accaccggcg gggtgggcag
      181 attgcggata ttcgagacaa tctgtcgaat ccgcgtgtct atggtcgccg ctggcgtttc
      241 cacgtgcgtg ctcactgctt cgtgcgtcat tcgtctttcg gggtctcggg ttgttccggc
      301 gaccgccggc acatttcgtc cgccaggcgg tcggcgatct atcgcttgat ctcgggccgg
      361 tcgtagtacc cggagcgcat ccggtcgctc gccagccgca tcttctccat cctcagtccc
      421 tcggcgtctt cggggggcgg cgtgaggatt tcctccgcgc gggcggcgtc ggccagcgcg
      481 gccagccgtg cccgggcgtc ctccgagatc accagttgat cgccgggcgt gccggccttc
      541 cgcgctcctc cgccctcctc cggcgcggcg ttgttcctgc ctttcctgcg caccgtcgtc
      601 tcatcgcccg gcgtccgcgc cgtgtcgagc gacggccgca ccgtccgaac ccttcctatg
      661 ttcatacggc tgcctcatgg tgtgaaagtc ctgcttctat ccggcgcacc cgccggcgtt
      721 accttcctat ggcgcgctcc gcgcgtcgcg ccgaccgcac ccgtggccct ctcatctctg
      781 cccccgcccg gcgggcgggg ccgactccct acttctttcc gtccacaaac tgcggcgacg
      841 gatccggttc gctgaggtac tggagaatct gctggctgtt gcgcagttcc ccgagctcct
      901 ccttgatccg gttgacccgt ccctgcaggt attcgtcgca ttcggacacc acgtcgacgc
      961 tctttttgac cagcgtccgg atgctgttga tcgtcctctt cacttccggg agcgccgcgg
     1021 cgagccggaa ggctttgggg tgtttttcct gaagctgccg gatcgtcaac tccgaccggg
     1081 cgattctctg gcggtagcgc tcgacctcgg ccagcagatc caggaggtgg tcgtcgcggt
     1141 tgaagcggac cgcgtcccgc tgcttgtcga gggtgaggta gagcgactgg tagaacgtat
     1201 actcttcttt gagcaccgcc agcagttcgc gttcgagttg tttgagcacg tcgtagtcca
     1261 tggcgctctc tcatgccgag ccgacgaaac cgccgggcgc ggccgccggg gccggcccgc
     1321 tcggcgccgg atcggccgat tgggcgcgaa ggccgatcca gccctcgcga aggttattga
     1381 gtatggcaat gttgtcgtca atcacgcccg ggtcgccggt ggccgcggcc acactgatcc
     1441 ggttgatcac gtaggcgtac agccgcccga gttgtgcgga gatgtcgccg ccccgctcgg
     1501 cgtccagggt cgtgtagagg tgggtgacga acgtctgcgc ccgctccagt tgctcccgac
     1561 cgccggccgc gtcgtcgctc cggtaccggt cggccgccgc ccggaaggca ctgatggccc
     1621 cgtcgtacac ctgaatcacc agttccaggg gcgacttccc cgccatctcc tcccgccggt
     1681 acgcggccac tttgctgctc gccatctgtt gttctcctcc cgaaggcact actgcttgct
     1741 cgttcgaaat ccccagttcg tgttgatgcc cgcgagttgc gattcgaggt agtttccgat
     1801 gctctgcatc tctccgagcg tctgctccat gaggtagtat ttctgcagga gcgactcgcg
     1861 ccggagcttg agccgctcgt cgatttccgc cacccgtttg gcgatcgtgt cgacctggga
     1921 ctggtagctc ttgatccgcc ggtcgaaagt cccctcctcg gccttggtca ggctctcgac
     1981 gacgtctttg aggcgggcgg ccacgccctt ggtcaccgtc acctgccctt cggcgccgga
     2041 gatcacctgc gtctcatcga gcgtcacccg gagcaccagg ccgtcggtgg tcgcgtttcc
     2101 cgtttttccg gtcagcaact gccccttgcc ctcggccgtc tcgccgttga tggttccggc
     2161 cacatcttgc ccggcgacgc tctctccggc ggcgagcccg agc
//
LOCUS       DAOOKS010000332         2200 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_62074_length_2200_cov_3.056410, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000332 DAOOKS010000000
VERSION     DAOOKS010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62074_length_2200_cov_3.056410"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            425..802
                     /locus_tag="PK112_05995"
     CDS             425..802
                     /locus_tag="PK112_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11633.1"
                     /translation="MKLLFVALVVATLLAAFYYFFVDTLTLTEIASSSGNRTAAAAAA
                     LVNFDTGLTRWDIRRLSDRASAWEERWRALDSLSDRSARRAGRDSLVSEMMADPAFKK
                     LSDRLGTLGERSAESILEFLSRR"
     gene            complement(858..2189)
                     /locus_tag="PK112_06000"
     CDS             complement(858..2189)
                     /locus_tag="PK112_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPC11634.1"
                     /translation="MNAPRRSILVVDDAPDTLEVVRRNLTAKGYRVYTAPDVGAATAL
                     LESTPVDLVITDLKMPRASGLDLVRHVRENLRDTEVMMMTGFASVTGAIEAMKTGAEE
                     YLVKPFTDEELFAAVKRSLGKLSTRRTAETPAAALEYAQFGILGDSPALRGVFRAIEK
                     AAGTSATVLISGESGTGKELVARAIHYRSPRASAPFVPVNCGGIPEGLLESELFGYVK
                     GAFTGANESRAGFFQTADGGTIFLDEIAETSLAMQVKLLRVLQDGEVYMVGATRSRRV
                     DVRIVAATNKDLEVLVGKDLFREDLFFRLSVITIAVPPLRERGDDVLVLARHFAEKFA
                     GEQGRTTPRLSDGAIEVFRTYNWPGNVRELENVVRRLVVMSDRDVVDIPDLPALMRYS
                     APKEGGVARRLAEVEGEYIRNVLARVGGNKTKAAEILGIDRKTLREKLKRG"
BASE COUNT          350 a          888 c          605 g          357 t
ORIGIN      
        1 cccggccgcg tcttttctag cgttgactcc gctccccgcc ctccgtatga tgagggggac
       61 agtatcgacc gattgcccga tccaccctcc ggccgcgccc caacggcggc cggcagaacg
      121 agaaacggag gaacaatcat ggccgacaaa ggaagtaagg accgggggaa gaaagaacag
      181 aagaagaagc cgcagcacac gcccgaagaa aaacgccgtc tcaagcggga gaagaaagaa
      241 aagaagtagg cggccggccc cggccaaacg atgtcgcaag gcgcgccggt gccgggcggg
      301 tcgggcacgt ttcccgtgcc cggcgctccc cggcgccggc ctttccgctt gcttcccgct
      361 cttcccccgc ctactttggt cccatgcgga aacccgctcg cccgtcgact ccggagattc
      421 gccgatgaaa ctcctcttcg tcgccctcgt cgtggccacc ctgctggccg ccttctacta
      481 cttcttcgtc gacaccctca ccctgactga aatcgcctcc tcctccggca accgcaccgc
      541 ggccgcggcg gccgccctcg tcaacttcga caccggcctc acgcgctggg atatccgccg
      601 cctgtccgac cgggcgtcgg cttgggagga gcgctggcgc gcgctggact ccctctccga
      661 ccgcagcgcg cgccgggccg ggcgcgactc gctcgtctcc gaaatgatgg ctgaccccgc
      721 cttcaagaaa ctctccgacc gcctcggaac tctcggcgaa cgctcggccg agtccattct
      781 cgagttcctc tcccgccgct gatctcccgg ctcccgctgg tcgggtccgt gcaatccgcc
      841 cccgctccct cgccccctca cccgcgcttc agtttctccc gcagcgtctt ccggtcaatc
      901 ccgaggatct ccgccgcctt cgtcttgttc cctccgaccc tcgcgagcac attccggatg
      961 tactctccct ccacctccgc cagccggcgc gccacccccc cctccttggg cgccgaatac
     1021 cgcatcagcg cgggaaggtc cgggatatcg accacatccc gatcgctcat caccaccagc
     1081 cgccgcacca cattctccag ttcccgcacg ttccccggcc agttgtaggt ccgaaagacc
     1141 tcgatcgcgc cgtccgacag ccgcggcgtt gtccgcccct gttcgccggc gaacttctcg
     1201 gcgaaatgcc gcgccagcac cagcacgtca tccccccgct cgcgcagcgg cggcaccgcg
     1261 atcgtgatca ccgacagccg gaagaagaga tcttcgcgga agagatcctt gccgacgagc
     1321 acctccagat ccttgttggt cgccgcgact atccgcacat ccacccgccg cgagcgcgtc
     1381 gccccgacca tgtacacctc tccgtcctgg agcacgcgca ggagcttcac ctgcatcgcg
     1441 aggctcgtct cggcgatctc gtccaggaag atcgtcccgc cgtcggcggt ctggaaaaac
     1501 cccgcccgcg attcgttggc ccccgtgaac gcgcctttga cgtacccgaa cagctcgctt
     1561 tccaggaggc cctccgggat ccccccgcag ttgaccggca cgaatggtgc ggaagcccgc
     1621 gggctgcggt agtggatcgc ccgcgccacc agctccttgc ccgtgccgct ctcgcccgaa
     1681 atcagcaccg tcgccgacgt ccccgccgct ttctcgatcg cccggaacac cccgcgcagc
     1741 gccggcgagt cccccaggat cccgaactgc gcgtactcga gcgccgccgc cggcgtctcc
     1801 gccgtccgcc gcgtcgacaa cttccccagc gaccgcttca cggccgcgaa cagctcctcg
     1861 tcggtgaagg gcttgacgag atactcctcc gcgcccgtct tcatcgcctc gatcgccccc
     1921 gtcaccgagg cgaaccccgt catcatcatg acctcggtgt cgcgaaggtt ctcgcgcacg
     1981 tggcgcacga gatccagccc gctcgcccgc ggcatcttga gatcggtgat caccagatcc
     2041 accggcgtcg actccaaaag cgctgtcgcc gccccgacat ccggcgccgt gtacacccgg
     2101 taccccttcg ccgtgaggtt ccgccgcacc acctccagcg tgtccggcgc gtcgtccacc
     2161 accagaatgg agcgacgggg cgcgttcatg cggctttcgc
//
LOCUS       DAOOKS010000333         2199 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_62105_length_2199_cov_3.586287, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000333 DAOOKS010000000
VERSION     DAOOKS010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62105_length_2199_cov_3.586287"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1061)
                     /gene="bshC"
                     /locus_tag="PK112_06005"
     CDS             complement(<1..1061)
                     /gene="bshC"
                     /locus_tag="PK112_06005"
                     /inference="COORDINATES: protein motif:HMM:NF021586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HPC11635.1"
                     /translation="MTPGKALGYSELYLDFVSGRETARHFYTPDNLSAVAARLDGAVY
                     ARDRTADILRRQNQAWGAGEPTLAAIERLRDPRAVAVFAGQQAGLFGGPLLVQIKALG
                     IIQAARRIGRELGRPAVPIFWIAADDHDFAEINHTWVPDRAAAEPVKIVYPTAPAEAA
                     PAAEIRFADAEALAAAKARLREALGETEFTPALYGLIDRCYTPEETFVGAFAKLMTAL
                     LGEFGLVLFSPADPEAKALAAPLLQALAERHEEVRALVGRTNENLRSHGYHVQVEKKD
                     ESVHLFFARDGRRPVLRQSGGGFRIGDRAVTAADLRAMIAQEPERFSPDVLTRPVMQS
                     WLFPAAAQHGGPSEIAYFA"
     gene            complement(1066..>2199)
                     /locus_tag="PK112_06010"
     CDS             complement(1066..>2199)
                     /locus_tag="PK112_06010"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPC11636.1"
                     /translation="IEISVQGPEHDEVVRLARRLQAYFYDVPGVVDVDNTLVEGKPEI
                     AVTYDRRLASDLGISLQDIPMTVRWLVEGDVVTRFKEGDEEYDVRMRLDERFRQSTEN
                     LGSILIKSDKSGSDGAPLLVPLERVAQLRKTAEISEYYRYNRQREIRVNANVTSTQFP
                     GTATNLIMEKFSAEVGASGGYVVAPVGQQEIMEESFRNILRALFLAVIFIYLLLASQY
                     ESFWDPFSIMLSLPLSLVGAIIGLIGSSFSIMSLIGIVLLMGLVTKNAILLIDFVKQE
                     RAKGVSRTDAILAAGPVRLRPIMMTTLAMAFGMLPLALGIGPGAELRAPMARAVIGGI
                     TSSMLLTLVVVPVVYTLIDDAVGLFRRKKAPGAVLKEDSTVRG"
BASE COUNT          374 a          812 c          677 g          336 t
ORIGIN      
        1 tgcgcgaaat aggcgatctc cgagggtccc ccgtgctggg ccgccgcggg aaacagccac
       61 gactgcatca ccggccgcgt caacacgtcg ggcgagaacc gctccggctc ctgcgcgatc
      121 atggcgcgca gatccgctgc cgtgaccgcc cggtccccga tgcggaagcc gccgccgctc
      181 tgccgcagca ccggccgccg gccgtcgcgc gcgaaaaaca ggtgcacaga ctcgtccttc
      241 ttctccacct ggacgtggta cccgtggctg cggaggttct cgttggtccg gccgaccagg
      301 gcgcgcactt cctcgtgccg ctccgcgagc gcctgaagga gcggcgccgc gagcgccttg
      361 gcctccggat ccgcgggcga gaacaggacg agcccgaatt cccccaggag cgcagtcatc
      421 agtttcgcaa aggcgccgac aaaggtctcc tccggagtgt agcagcggtc gatcagcccg
      481 tagagggccg gggtgaattc cgtctccccg agtgcttcgc gcaggcgcgc tttggccgcc
      541 gcgagcgcct cggcgtcggc aaaacggatc tccgccgccg gcgccgcctc cgccggagcc
      601 gtcgggtaga cgatcttcac gggctcggcc gcggcccggt ccggcaccca ggtgtggttg
      661 atttccgcga aatcgtggtc gtcggcggca atccagaaaa tcggcacggc gggccgcccg
      721 agctcgcggc cgatccgccg cgccgcctgg atgatcccga gcgccttgat ctgcaccagc
      781 agcggcccgc cgaacagccc cgcctgctgc cccgcgaaca ccgccaccgc ccgcgggtcg
      841 cgcagccgct cgatcgccgc cagcgtcggc tcgcccgccc cccaggcctg gttctgccgc
      901 cgcaggatgt ccgccgtccg gtcgcgcgca tagacggcgc cgtcgagccg cgcggccacg
      961 gccgacaggt tgtcgggcgt ataaaagtgc cgggccgttt cccggcccga cacgaaatca
     1021 agatacagtt cgctgtagcc gagcgccttg cccggcgtca ccagtctaac cgcggacagt
     1081 cgaatcctcc ttcagcaccg cccccggcgc ctttttgcgc cggaacaggc cgacagcatc
     1141 gtcgatcagc gtgtacacca ccggcaccac cacgagcgtg agcagcatcg acgaggtaat
     1201 gccgccgatg accgcacgcg ccatcggggc gcgcagttcc gcgcccggac caatcccgag
     1261 cgccagcggc aacatcccga acgccatcgc cagcgtcgtc atcatgatcg gccgcaaccg
     1321 gaccggcccg gccgccagga tcgcatccgt ccttgaaaca cctttcgccc gctcttgttt
     1381 cacaaaatca atcagcagaa tcgcgttctt cgtcaccagc cccatcagga gcacgatccc
     1441 gatgagcgac atgatcgaaa acgacgatcc gatcagcccg atgatcgccc ccaccagcga
     1501 gagcggcaga gacagcatga tcgagaacgg atcccagaac gactcgtact gcgaggcgag
     1561 gagcaggtag atgaagatga ccgcgagaaa cagcgcccgc aggatgttgc ggaacgactc
     1621 ctccatgatc tcctgctggc ccaccggcgc caccacgtac ccgcccgacg cccccacctc
     1681 ggcggagaat ttctccatga tcaggttggt cgccgtcccc gggaactgcg tactcgtcac
     1741 gttggcgttg acccggatct cccgctgccg gttgtagcgg tagtactcgc tgatttccgc
     1801 cgtcttccgc agttgggcca cgcgctcgag cgggaccagc agcggcgccc cgtcggaccc
     1861 gctcttgtcg ctcttgatga ggatgctgcc gagattctcc gtcgactgcc ggaaccgctc
     1921 gtccagccgc atgcggacgt cgtactcctc gtccccctcc ttaaaccgcg tgaccacgtc
     1981 cccctcgacc agccaccgga ccgtcatcgg aatatcctgg agcgagatcc cgaggtcgct
     2041 ggcgaggcgg cggtcgtagg tgacggcgat ttcgggtttt ccctcgacga gcgtgttgtc
     2101 aacgtcgacc acccccggca cgtcgtagaa gtaggcctgg aggcgacggg cgagccgcac
     2161 gacctcgtcg tgctcgggcc cctggacgga aatctcaat
//
LOCUS       DAOOKS010000334         2199 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_62110_length_2199_cov_3.369403, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000334 DAOOKS010000000
VERSION     DAOOKS010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62110_length_2199_cov_3.369403"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..862)
                     /gene="meaB"
                     /locus_tag="PK112_06015"
     CDS             complement(<1..862)
                     /gene="meaB"
                     /locus_tag="PK112_06015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HPC11637.1"
                     /translation="MTVLERFRNGDVRALSRLVSFVEDQENGYRDLLGRLYASVGKSL
                     RIGVTGPPGAGKSTLVNGLTHELLRENRRVGIIAVDPTSPFTGGALLGDRVRMNEFPA
                     DGRVYFRSMATRGATGGLAAATNNVAVVYDSFGFDVTLIETVGVGQVELDIINACDVV
                     VVVMVPESGDAVQAMKAGVMEIADVFCVNKSDRPGAERIAHELRYALETRRQTEDAWK
                     VPIVLTEGLSRKGVDQLLERINAFVAFMKARGRFEQHRRSQIADQIINVLRYRFQREF
                     IDKIQSAEEFR"
     gene            complement(867..>2199)
                     /gene="recJ"
                     /locus_tag="PK112_06020"
     CDS             complement(867..>2199)
                     /gene="recJ"
                     /locus_tag="PK112_06020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HPC11638.1"
                     /translation="KNNGVSLIITVDTGITAVEEIKYANSIGIDVLVTDHHEPGGDLP
                     AAYTIINPKQIACDYKSELSGVGVAFKFCQALYRALNQDETELWEHLDLVALGTSADI
                     VPLVNENRILTKFGINQISRTTKPGLKSLTFVAGLMGKDISTGQVVFILAPRINALGR
                     LGDAGKAIRLLATRDERAAAEIARKLDSENKRRKEIDETTLEEALAQMDDVCDLEADR
                     AIVLAQNDWHQGVIGIVASRLVERFHLPTVMISIVNGEGKGSARSIPGFHLCEALKEC
                     EHLLIRYGGHKYAAGLSIAPDKVEEFRRLFVEVSNRILSEEDITPKMHIDLEIELSDI
                     DDHFMNAIEAFAPFGPQNMRPVFLTRNCEVVGQPYVVGNNHLKMRLRKGDTQFDVIGF
                     GFGDMARMISARGCLVDAVYVVEYNTYNGVTRKQIRLKDLRLTAGTGPVPL"
BASE COUNT          343 a          604 c          832 g          420 t
ORIGIN      
        1 tgcggaattc ctcggccgac tggatcttgt cgatgaactc gcgctggaaa cggtagcgga
       61 ggacgttgat gatctggtcg gcgatctggc tgcgccggtg ctgctcgaac cggccgcggg
      121 ctttcatgaa agcgacgaaa gcgttgatgc gttcgagcag ttggtccacc cccttgcggc
      181 tgagcccttc ggtgagcacg atcgggactt tccaggcgtc ctcggtctgg cggcgcgtct
      241 cgagggcgta gcggagctcg tgagcgatgc gctcggcgcc cgggcggtcg gacttgttga
      301 cgcagaagac gtcggcgatc tccatcacac ccgccttcat cgcctggacc gcgtcgcccg
      361 actcgggcac catgaccacc accaccacgt cgcaggcgtt gatgatgtcg agttcgacct
      421 ggccgacgcc gacggtctcg atgagggtga cgtcgaagcc gaagctgtcg tagacgacgg
      481 cgacgttatt ggtggcggcg gcgaggccgc cggtggcgcc gcgcgtcgcc atcgagcgaa
      541 aatacacccg gccgtcggcc ggaaactcgt tcatgcggac gcggtcgccg aggagggcgc
      601 cgccggtgaa aggggaggtc gggtcgacgg cgatgatgcc gacgcggcgg ttctccctga
      661 gcagttcgtg ggtgaggccg ttgacgaggg tcgacttgcc ggcgccgggc gggccggtga
      721 cgccgatgcg gagggatttg ccgacgctcg cgtagagccg cccaagcaga tcgcggtagc
      781 cgttctcctg atcctcgacg aaggagacga ggcgcgagag ggcgcggacg tcgccgttgc
      841 ggaagcgttc gagtacggtc atggcgtcac agcggcaccg gcccggtgcc ggcggtcagg
      901 cgcaggtctt tcaggcgaat ctgcttgcgc gtgacgccgt tgtaggtgtt gtactcgacg
      961 acgtagacgg cgtcgacgag acagccgcgc gccgagatca tgcgggccat gtcgccgaag
     1021 ccgaaaccga tgacgtcgaa ctgggtgtcg cccttccgga ggcgcatttt caagtggttg
     1081 ttgccgacca cgtagggctg gccgaccacc tcgcagttgc gggtgagaaa caccgggcgc
     1141 atgttctggg gaccgaaggg ggcgaaggcc tcgatggcgt tcatgaagtg gtcgtcgatg
     1201 tcggacagct cgatttcgag gtcgatgtgc atcttgggcg tgatgtcttc ctcggagagg
     1261 atccggttgg agacctcgac gaagagccgt cggaactcct cgactttgtc gggggcaatg
     1321 gacaggccgg cggcgtactt gtggccgccg tagcggatga ggaggtgctc gcactccttg
     1381 agggcctcgc agaggtggaa gccggggatg gagcgggcgg agcctttgcc ctcgccgttg
     1441 acgatggaga tcatgaccgt ggggaggtgg aagcgctcga cgagacggga ggcgacgatg
     1501 ccgatgacgc cctggtgcca gtcgttctgg gcgaggacga tggcgcggtc ggcctcgagg
     1561 tcgcatacgt cgtccatctg ggcgagggcc tcctcgaggg tggtttcgtc gatttctttg
     1621 cggcgcttat tttcgctgtc gagtttgcgg gcgatttcgg ccgcggcgcg ctcgtcgcgc
     1681 gtcgccagca ggcggatcgc cttgcccgca tcccccaggc ggccgagggc gttgatccgc
     1741 ggagccagga tgaagacgac ctggccggtg ctgatgtctt tgcccatgag gccggccacg
     1801 aaggtgaggg atttcagtcc gggtttggtg gtgcgggaga tctggttgat gccgaacttg
     1861 gtgaggatgc ggttctcgtt gacgagcggg acaatgtcgg cggaggtgcc gagggcgacg
     1921 aggtcgaggt gctcccagag ttcggtttca tcctggttga gggcgcggta gagggcctgg
     1981 cagaatttga aggcgacgcc gacgccggag agctcgctct tgtagtcgca ggcgatctgc
     2041 ttggggttga tgatggtgta ggccgcgggc aggtcgccgc ccggttcgtg gtggtcggtg
     2101 acgagaacgt cgataccaat ggagttggcg tatttgatct cctcgacggc ggtgatgccg
     2161 gtgtccacgg tgatgatgag gctgacgccg ttgttcttg
//
LOCUS       DAOOKS010000335         2192 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_62413_length_2192_cov_2.878802, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000335 DAOOKS010000000
VERSION     DAOOKS010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62413_length_2192_cov_2.878802"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="PK112_06025"
     CDS             complement(<1..304)
                     /locus_tag="PK112_06025"
                     /inference="COORDINATES: protein motif:HMM:NF021235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HPC11639.1"
                     /translation="MRVTVCAIAVLLPLAAAAGQWHTGESLMCQDCHLQHSSEQGEPV
                     AGGPYSVLLLKSSINELCLSCHDGSNPTAPDVQSPVQMYQGTPSGESAGGWFGLLGA"
     gene            complement(313..1275)
                     /locus_tag="PK112_06030"
     CDS             complement(313..1275)
                     /locus_tag="PK112_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11640.1"
                     /translation="MERKRIDTEAQGLAGSSSPRARCRRGPAIGAAALSAVFLFAAAV
                     SAQTLDGEIVIAADGLRLERPSSLAVSPSGEQIAVADPQANRIAVIDYFGRPLWSAGG
                     AVDLGRPLAVAFTGENELVFTRTADLRILRLIRDNPAELDSIADLAPVLEPHRRLDQL
                     LRTPERGWLALDRKTGEVLRFDESWGYQGIAVPHGSGKGKVLVPSALVPLADGRFVVT
                     DEKNYPAQLFSAEGKFLLYFGWSSPGEERGWEAAGGTADSRSLIWVADRTAAAFRIFD
                     PAGGAVAAVPFPQPLFRPAAMTTTIDNRVLVLDEFGRALFYRVQ"
     gene            complement(1296..>2192)
                     /locus_tag="PK112_06035"
     CDS             complement(1296..>2192)
                     /locus_tag="PK112_06035"
                     /inference="COORDINATES: protein motif:HMM:NF025890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HPC11641.1"
                     /translation="PHASAFTALKNKPAETFCLACHADLEQQIKNGQPHAPVVSGECT
                     ACHQPHAGATASLLTVSKENLCGSCHDASAGEMTAAHSGFAVAAADCQNCHEAHAAPK
                     GGGGLLMPRAHAPFTAGQCGSCHVGKQPARLIAPVKTLCLTCHEDFARTLDRPVVHAP
                     VGDDNGCTGCHGPHVGYTGALQKKQGVAVCLTCHNTPEFSGKFKHQAAFEDCRTCHEV
                     HAGDYKRLLSTPDIMELCMTCHADAKETHYHPMGAGVIDPRTKQDLNCVGCHSPHSSE
                     YDKILLGDRDRKLCITCHSLSH"
BASE COUNT          335 a          706 c          805 g          346 t
ORIGIN      
        1 cggcgcccaa aagaccgaac cagccgcccg cgctctcccc ggagggcgtg ccctggtaca
       61 tctggacggg gctctggaca tcgggggcgg tcggattgct cccgtcgtgg cacgacagac
      121 acagctcgtt gatcgagctt ttcagcagga ggaccgagta cggtccgccg gcgaccggtt
      181 ccccctgttc agaggagtgc tgcaggtggc agtcctggca catcaggctc tcccccgtgt
      241 gccactggcc cgcggccgcg gccaggggaa gaaggacggc gatggcgcag acagtaactc
      301 gcatggccgt tctcactgca cgcggtagaa gagggcgcgc ccgaactcgt cgaggacgag
      361 cacgcggtta tcgatggtgg tggtcatcgc ggccggccga aagagcggct ggggaaaggg
      421 cacggcggcg accgcgccgc cggcgggatc gaagatgcgg aaggcggccg cggtgcggtc
      481 ggccacccag atcaggctgc ggctgtcggc cgtgccgccg gcggcctccc agccgcgctc
      541 ctcgccgggg ctgctccagc cgaaatacag gaggaatttc ccctcggcgg agaaaagctg
      601 ggccgggtag ttcttctcgt cggtcaccac gaaccggccg tcggccagcg gcacgagcgc
      661 gctcgggacc aggactttcc ccttgcccga accgtgggga accgcgatcc cctgatagcc
      721 ccagctctcg tcgaagcgca gaacctcccc cgtcttccgg tcgagcgcca gccagccgcg
      781 ctcgggggtg cggaggagct ggtccagccg gcggtgcggt tcgagaaccg gcgcgagatc
      841 ggctattgag tcgagttcgg ccgggttgtc gcggatcagc cgcaggatgc gcagatcagc
      901 tgtgcgggtg aagacgagtt cattttcgcc ggtgaaagcc accgcgagcg ggcggcccag
      961 gtcgacggcg ccgccggccg accagagcgg gcggccgaag tagtcgatga cggcgatccg
     1021 gttggcctgg ggatcggcca ccgcgatctg ctcgccgctc ggcgacacgg cgagcgagct
     1081 ggggcgttcg aggcgaaggc cgtcggccgc aatgacaatc tcgccgtcga gcgtctgggc
     1141 ggagacggcc gcggcgaaca ggaacacggc gctaagggcg gccgcaccaa tcgccggccc
     1201 tcgccgacac cgggcgcgcg gcgatgaact gccggcgagc ccctgtgctt ccgtgtcgat
     1261 tcgtttccgc tccatggtcg tcaccccggc cgccctcaat gcgagaggct gtggcaggtg
     1321 atacagagtt tccggtcgcg atcgccgagg aggatcttgt cgtactccga ggagtgcggc
     1381 gaatggcagc cgacacaatt gagatcctgc ttggtccgcg ggtcgatgac gccggcgccc
     1441 atcgggtggt agtgcgtctc tttcgcgtcg gcgtggcagg tcatgcacag ctccatgatg
     1501 tcgggggtcg agaggaggcg cttgtagtcg ccggcgtgga cctcatggca ggtgcggcag
     1561 tcttcgaaag ccgcctggtg tttgaacttc ccgctgaact ccggcgtgtt gtggcaggtc
     1621 aggcagacgg ccacgccctg ctttttctgc agcgcgccgg tgtagccgac gtgtggaccg
     1681 tggcagccgg tgcagccgtt gtcgtcgccg accggcgcgt gcacgaccgg ccggtcgagc
     1741 gtcctggcaa aatcctcgtg gcaggtcagg cacagcgtct tgaccggcgc gataagccgg
     1801 gcgggctgtt tgccgacgtg gcaggaaccg cactggccgg ccgtgaaggg ggcgtgcgct
     1861 ctgggcatga ggagaccccc gccgcctttc ggggcggcgt gcgcctcgtg gcaattctga
     1921 cagtcggccg ccgcgaccgc aaaacccgaa tgcgcggccg tcatctcccc cgccgaggcg
     1981 tcgtgacacg aaccgcagag gttctctttc gaaacggtca gcagcgacgc ggtcgcgccg
     2041 gcatggggct ggtggcaggc ggtgcattcg ccgctcacca ccggggcgtg cggctggccg
     2101 ttcttgatct gctgctcgag gtcggcgtgg caggcgagac agaacgtctc cgccggcttg
     2161 ttcttgagcg ccgtgaaggc cgaggcgtgc gg
//
LOCUS       DAOOKS010000336         2191 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_62449_length_2191_cov_3.143258, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000336 DAOOKS010000000
VERSION     DAOOKS010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62449_length_2191_cov_3.143258"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="PK112_06040"
     CDS             <1..443
                     /locus_tag="PK112_06040"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HPC11642.1"
                     /translation="AAAAPGLFAGRPWFIDETVRLSVSHRARWNWLRAAVGATPVLID
                     SALHDELMAELSHLPQLLATVLGAQTDPRTVELAGPGLASVLRLAGSPHAVWAEIIEA
                     NRAPILAALRLYRDNLNTVMDRIARGESLRDVFAAAARSYRCLS"
     gene            431..1447
                     /gene="aroF"
                     /locus_tag="PK112_06045"
     CDS             431..1447
                     /gene="aroF"
                     /locus_tag="PK112_06045"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HPC11643.1"
                     /translation="MLVVMEISASREAVENVCRLIGEMGLTAHAMPGAQRTAIGVTGN
                     KSQIDADRILTQPGVKEIIHVTKPYKLVSREFIPEDTVVDVGGVPVGGPEFAVMAGPC
                     SVESREQTLAVAEAVSRLGARILRGGAYKPRTSPYSFQGLKEPGLKILAEARERYGLK
                     IVTEAMDTDTIDLVADYADIIQIGARNMQNYSLLRRAGQKRKPVLLKRGLAATVDELL
                     MSAEYIMAEGNRQVILCERGVRTFADHTRNTLDLSAIPYVKRASHLPILADPSHGTGR
                     KDKVLPLSRASLAVGADGLLIEVHCDPAKALSDGPQALTPDLFAQLMKELRLMAPVLG
                     RTMA"
     gene            1444..>2191
                     /locus_tag="PK112_06050"
     CDS             1444..>2191
                     /locus_tag="PK112_06050"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11644.1"
                     /translation="MRTLLPGRIADTVLRVPGDKSIGHRAAVLALLAEGPLTIRNFPH
                     SADCHASLEAVRALGATVADDGLELVLTPPPELRADPDTVINCGNSGTTARLLAGLLA
                     GSCVEVTLSGDESLRRRPMARIIDPLAAMGATIAASDGHLPMRVRGGRLLPFEYTLPV
                     ASAQVKSALLLAGLASRCAVTIRETAITRDHTEILLAELGEGLAVRKVRPVLVADPHD
                     PRKRRQEMPEPFKREITLASRAVVRGGTVDI"
BASE COUNT          338 a          773 c          733 g          347 t
ORIGIN      
        1 cggcggccgc ggcccccggt ctatttgcgg gccgcccgtg gtttatcgac gaaaccgtcc
       61 gcctgtccgt ctcacaccgg gcccgctgga actggctgcg ggccgccgtt ggcgcgaccc
      121 cggtgctcat cgacagcgcc ctgcacgatg aactgatggc cgagctgtcc catttgccgc
      181 agctgctggc gaccgttctc ggtgcccaga ccgatccccg cacggtcgaa ctggcgggac
      241 ccggactggc ctccgtgctc cggctggccg gttccccgca cgctgtctgg gcggagatca
      301 tcgaggccaa ccgagctccg atcctcgccg cgctgcgcct gtaccgggac aacctgaaca
      361 ccgtcatgga ccggatcgcc cggggcgaat cgttgcgcga cgttttcgcc gcggccgcca
      421 ggagttatcg atgcttgtcg tgatggagat atccgcctcg cgcgaggccg tggagaatgt
      481 ctgccgcctg atcggggaga tggggctgac cgcccacgcg atgccgggcg cccagcgcac
      541 ggcgatcggc gtgaccggca acaagtcgca gatcgacgcc gaccgcatcc tcacccagcc
      601 gggcgtcaaa gagatcatcc acgtcaccaa gccctacaag ctggtgagcc gggagtttat
      661 cccagaagat acggtggtcg acgtcggcgg cgtgccggtc ggcggcccgg agtttgccgt
      721 catggccgga ccctgttcgg tcgagagccg cgagcagacg ctggcggtgg ccgaggcggt
      781 ctcccggctg ggagcccgga tcctgcgggg cggcgcctac aaaccccgca cctcgcccta
      841 ttcgttccag gggctgaagg aacccggcct caagatcctc gccgaggcgc gcgagcgcta
      901 cggtctgaag atcgtcaccg aggccatgga caccgatacg atcgacctgg tggccgacta
      961 cgccgacatc atccaaatcg gcgcccgcaa catgcagaac tactcgctcc tgcggcgggc
     1021 cgggcagaag cgcaaacccg tcctgctcaa gcgggggctt gcggcgacgg tcgatgaact
     1081 gctcatgtcc gcggagtaca tcatggccga gggcaaccgc caggtgattc tctgcgagcg
     1141 tggcgtgcgc acgttcgccg accacacccg caacactctc gacctgtcgg ccattcccta
     1201 tgtcaagcgg gcgagccacc tgccgatcct cgccgacccg agccacggaa ccggccgcaa
     1261 ggacaaggtt ctcccgctct cgcgggcctc gctggcggtg ggggcggacg gcctgctgat
     1321 cgaggtgcac tgcgatcccg ccaaggccct ctcggacggt ccgcaggcgc tgacgcccga
     1381 cctgttcgcg cagctgatga aagaacttcg tctcatggcg ccggtactcg gcaggacgat
     1441 ggcatgagga cgttgctgcc gggccggatc gccgacaccg tcctccgcgt gccgggggac
     1501 aagtcgatcg gccaccgggc cgcggtgctg gcactgctgg ccgagggtcc gctcaccatc
     1561 cgcaacttcc cgcattccgc cgactgccac gcctccctcg aggcggttcg ggcgctgggg
     1621 gcgacggttg ccgatgacgg cctggagctg gttttgaccc cgccgccgga actgcgcgcg
     1681 gatcctgaca cggtcatcaa ttgcggaaac tccggcacga ctgcccgcct cctggccggg
     1741 cttctcgccg gctcgtgcgt cgaagtgacg ctcagcggcg atgagtcgct gcgccgccgc
     1801 ccgatggcgc ggattatcga cccgctggcg gcgatggggg cgacgatcgc cgcctccgac
     1861 ggtcatctcc ccatgcgtgt ccggggcggc agactgctgc ctttcgagta tacgctcccg
     1921 gtcgcctcgg cgcaggtgaa gtcggcgctc cttttggcgg ggctggcctc ccgctgcgcc
     1981 gtcaccatcc gcgagaccgc gatcacccgc gaccacaccg agattttgct ggccgaacta
     2041 ggggaggggc tcgcggtgcg gaaagtcaga ccggtgctgg ttgccgaccc gcacgatccc
     2101 cgcaagcgcc ggcaggaaat gccggagccg ttcaaacggg aaatcacgct tgcctcgcgg
     2161 gcggtggtcc gcggcggcac agtcgacatt c
//
LOCUS       DAOOKS010000337         2189 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_62553_length_2189_cov_2.815839, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000337 DAOOKS010000000
VERSION     DAOOKS010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62553_length_2189_cov_2.815839"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1519
                     /locus_tag="PK112_06055"
     CDS             <1..1519
                     /locus_tag="PK112_06055"
                     /inference="COORDINATES: protein motif:HMM:NF025665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HPC11645.1"
                     /translation="RFLPALDLPEAAYVPEPKPVRGAFEVGVYYFPGWNSSERWQPLR
                     AYPERTPVLGFYREGDPEVADWHIKFAVEHGITFFCYDWYWNRGDTQLTHALHDGYFK
                     ARYRSLLKFCLLWANHAPTVHSPEDNAAVCRYWIENYFRRPEYFKIDARPLLVIFDPG
                     SMKRDLGIAGSREAIALWHRMTREGGAGEVLVAGCGTPGAALEDLKAMGFDAVTGYNW
                     PTCGAGGRNRVPYAEIARAQYDAWWLPMARRNLLPVIVPASPGWDSRPWHGAKAFVQT
                     ERSPAAFAEHLTLAARFAQETASPRVVLIEAWNEWGEGSYCEPHKEYGFAHLDAVRRV
                     FCPGAEAHTDIGPGDVGLGPYDVAPPPPAATAWFFHREGDTRGWSALMGIAGLAAADG
                     VLRARTTTQDPALVCATRAPAREFTAMEIRMAVRNARPGDELQLFWRSPFAAMSEAAS
                     MRAALRDGGAMHSYRLDLGAHARWRGTVTQLRLDPCSTRGAEIAIESIRLLAPGS"
     gene            1968..>2189
                     /locus_tag="PK112_06060"
     CDS             1968..>2189
                     /locus_tag="PK112_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11646.1"
                     /translation="MKRAWLAAGLLVCSSALLARGGWEDPPGGWVYVYDADPGQDAYM
                     PGFWQIGCLDGEWMRGQSSDQWDGNAPGEG"
BASE COUNT          312 a          789 c          756 g          332 t
ORIGIN      
        1 gcgcttcctc cccgccctcg accttcccga ggcggcctac gttcccgaac cgaagccggt
       61 ccgcggggcg ttcgaggtgg gcgtctacta ctttccggga tggaattcct ccgagcgctg
      121 gcagccgctc cgcgcgtatc ccgagcgcac gccggttctc gggttctacc gcgagggcga
      181 ccctgaggtc gccgactggc acatcaagtt cgcggtcgag cacggcatca cgtttttctg
      241 ctacgactgg tactggaacc ggggcgatac gcagctcacc cacgcgctcc atgacggcta
      301 cttcaaggcg cgctaccgga gcctgctcaa gttctgtctg ctctgggcca accacgcgcc
      361 cacggtgcac tcgcccgagg acaatgccgc cgtctgccgc tactggatcg agaactactt
      421 caggcggccg gagtatttca agatcgacgc caggccgctc ctggtgatct tcgatcccgg
      481 gagcatgaag cgcgacctag ggatcgcggg cagccgcgag gccatcgccc tctggcacag
      541 gatgacgcgc gaagggggcg ccggggaggt gctcgtcgcc ggctgcggca ccccgggcgc
      601 cgccctggag gacctcaagg ccatggggtt cgacgcggtg accggctaca actggccgac
      661 ctgcggagcc ggcggccgca accgcgtccc gtacgcggag atcgcccgcg cccagtacga
      721 cgcctggtgg ctcccaatgg cccgccgaaa cctcctgccg gtcatcgtgc cggcgagccc
      781 gggctgggac tcgcggccgt ggcacggcgc caaggcgttc gtccaaaccg agcgctcgcc
      841 cgcggccttc gccgagcacc tgacccttgc ggcgcgcttc gcccaggaga ccgcgtcgcc
      901 cagggtcgtc ctcatcgagg cttggaacga atggggcgag ggttcgtact gcgaacctca
      961 caaggagtac gggttcgcgc acctggacgc cgtgcgccgg gttttctgcc ccggcgccga
     1021 ggcgcacacc gacatcggtc ccggcgacgt ggggctcggg ccctacgatg tcgcgccgcc
     1081 cccgcccgcg gcgaccgcgt ggtttttcca cagggagggc gacacacgcg gctggtcggc
     1141 cctgatgggc atcgccggtc ttgccgccgc ggacggcgtt ctccgcgccc ggacgaccac
     1201 gcaagacccg gcgctcgtct gcgcgacgcg cgcccccgcc cgggagttca cggccatgga
     1261 gatccgcatg gcggtgcgca acgcccgtcc gggcgacgag ctccagcttt tctggaggtc
     1321 gcccttcgcc gccatgagcg aggcggccag catgcgcgcc gcgctccggg acggcggcgc
     1381 catgcactcc taccggctcg atctgggcgc ccacgcgcgc tggcgcggca ccgtgacgca
     1441 gctccgcctc gacccatgct cgacgcgagg cgccgagatc gccatcgagt cgatccgcct
     1501 gctcgccccc ggatcgtgac cgccggcggc gccgcgccaa gggatcggcc acccccgccg
     1561 ggtctcgcgc accccgcggc cggcgctgtt ttcccttgat ttcgcggccg tttcgcgcct
     1621 acaatcacga tatacgacgg taatcgatcg gtttgggcag gcggctaccg cggtcctcga
     1681 gctcttttcg cgccaagagg acaaaccatg aaatgtggcc gcgagtcggg cgcggccggc
     1741 acgaacgcgc cggtcgaagg cggcgtgacg ccggtcgtcc ggatgcgcgc ggccgccgac
     1801 tcccacctcg atcccttggc ccgagcccga gcagcggcga gggccggcgt gctgcgatcc
     1861 acggaagggc gggcggcccg tgccgtccac ccccttgtcg tctattcccg ccggcggacg
     1921 cggcgggcat ttggagcctc ccaaagggga ggagaaggga gattgccatg aaacgcgcat
     1981 ggttggcggc gggtctgctc gtttgctctt cggcgctgct tgcccgcgga ggatgggaag
     2041 acccgcccgg cgggtgggtc tatgtctacg atgccgaccc cgggcaggat gcctacatgc
     2101 cgggcttctg gcagataggc tgcctcgacg gggagtggat gcgcggacag tcatcggatc
     2161 aatgggacgg caacgcgccc ggcgagggc
//
LOCUS       DAOOKS010000338         2185 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_62700_length_2185_cov_4.435681, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000338 DAOOKS010000000
VERSION     DAOOKS010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62700_length_2185_cov_4.435681"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..986)
                     /locus_tag="PK112_06065"
     CDS             complement(<1..986)
                     /locus_tag="PK112_06065"
                     /inference="COORDINATES: protein motif:HMM:NF012662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11647.1"
                     /translation="MIIKSFTAESASAAMKQARREMGGDAIVLKTRQLTDRYNRPQVE
                     ITACLEKPTVGRMSDILDSRPASVNRLPVASDGEPAPAAVEDAGPEPLADEPGAEAPV
                     PSEDRLARIEATLDRLVCLSATRGGEEIADPAIRAVAASLRDADVPEDFIDAFLLARP
                     ERFLGPGALAKIRSELVKELSRLMVPDLKFRPGDRVLFVGPAGAGKSSVMGKLAAHLA
                     AVEKRKVALAGVDFQKVGAHEELAGYADLLNLDVAEVAGEHADKAGTKDSITLIDAPA
                     LPAEPDRREALVRAAAKIGARYRFLVIPATMRSADAIDLAARYHWFGPTHLAA"
     gene            complement(976..>2185)
                     /locus_tag="PK112_06070"
     CDS             complement(976..>2185)
                     /locus_tag="PK112_06070"
                     /inference="COORDINATES: protein motif:HMM:NF012973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HPC11648.1"
                     /translation="IFGFLPGMPTFTCLALGGVVGGLGYLTTQLQAKRAVEEEEAKKR
                     SRAQEHRTQERTEDLLKVDTLGLEIGYGLIPLVDPNQRGDLLDRIGSIRKQLAAELGI
                     VVPPIRIRDNIRLRPNEYQIKVKGLRVAGFELMTDHLLAINPGYVREKLEGFDTKDPA
                     FGLAVTWILPNLREVAEAREYTVVEPSAVVATHLIETIRRAAPEILSRQDVQHLVDTL
                     KEDYPALVESTVPEIVPLGTMQKVLQQLLRERIPVRDLATILETVADYIGATKDADVL
                     AEYARMALRRQVTELAKDARGRISVFTIDPAVEQTLADSVQNTKQGLMLAIDPALAEQ
                     LLGRIGEQIDLQHAAGVNPACLCSPNIRLALRRLAEARFPGLMVLSYNEILPDVEVIS
                     TGMVRLQDDH"
BASE COUNT          322 a          691 c          794 g          378 t
ORIGIN      
        1 gccgccaggt gggtgggccc gaaccagtgg tagcgggccg ccagatcgat cgcatcggcg
       61 gagcgcatcg tcgccgggat caccaggaac cggtagcggg cgccgatttt cgccgccgcc
      121 cgcacgagcg cctcgcggcg atcgggctcg gccggcagcg ccggagcatc gatcagcgtg
      181 atgctatctt tggtcccggc cttgtccgcg tgctcgccgg cgacttcggc gacatcgagg
      241 ttgagcaggt cggcgtagcc ggccagttct tcatgggcgc cgactttctg aaaatccacg
      301 ccggccagcg ctaccttccg tttctccacg gcagcgaggt gcgcggccag tttgcccatg
      361 accgaggact tccccgcccc ggccggaccg acaaagagga cgcggtcgcc cgggcggaac
      421 ttcagatccg gcaccatcag ccgggacagc tccttcacca gctcgctgcg gattttcgcc
      481 agcgcgccgg ggccgaggaa ccgttccggc ctggccagca ggaaagcatc gatgaaatct
      541 tccggcacat cggcgtcgcg gagggaagcg gcgaccgccc ggatcgcggg gtcggcgatt
      601 tcctcacccc cgcgcgtcgc gctgaggcag acgaggcggt cgagggttgc ctcgatgcgg
      661 gcgagccgat cctcggaggg gacgggtgct tcggcccccg gttcgtcggc aagcggctcg
      721 ggccccgcgt cttcgaccgc ggccggggcc ggctcgccgt cggaagcgac ggggagccgg
      781 ttgaccgaag ctgggcggct gtcgaggatg tcggacatcc ggccgacagt cggtttttcc
      841 aggcaggcgg tgatttccac ctggggccgg ttgtaccggt cggtgagctg gcgcgtcttg
      901 agcacgatgg cgtcgccgcc catttcccgg cgcgcctgtt tcatggcggc cgaggcggat
      961 tctgcggtaa acgacttaat gatcatcctg caacctcacc attccggtcg agatcacctc
     1021 gacgtcgggg agaatctcgt tgtacgagag gaccatgagg cccgggaagc gcgcctcggc
     1081 gaggcggcgc agggccagtc ggatattggg cgaacagaga caggccgggt tgacgccggc
     1141 cgcgtgctgg aggtcgatct gctccccgat ccggccgagc aattgctcgg ccagcgccgg
     1201 gtcgatcgcg agcatcagtc cctgtttggt attctgcacg gagtcggcga gtgtctgctc
     1261 caccgccggg tcgatggtga agacgctgat ccgcccgcgg gcgtctttcg ccagttccgt
     1321 cacctggcgc cggagcgcca tgcgggcgta ctcggccagc acgtcggcgt ccttggtcgc
     1381 gccgatgtag tcggcgacgg tctcgagaat ggttgccagg tcgcgcaccg ggattcgctc
     1441 gcgcaggagc tgctgcagga ctttctgcat cgtgccgagc gggacgatct ccggcacggt
     1501 ggactcgacc aaggccgggt agtcttcctt gagcgtgtcg acgaggtgct ggacgtcctg
     1561 gcgcgagaga atttccgggg cagcccggcg gatcgtctcg atcaggtgcg tggcgaccac
     1621 ggccgagggc tcgactacgg tgtactcgcg ggcctcggcc acctcgcgga ggttcgggag
     1681 aatccaggtc acggcgaggc cgaacgccgg atccttggtg tcgaaaccct cgagcttctc
     1741 gcggacatag ccgggattga tggcaaggag gtggtcggtc atgagttcaa agccggcgac
     1801 gcggagaccc ttgaccttga tctggtattc attcggccgg agccggatgt tgtcgcggat
     1861 gcggatgggc gggacgacga tgcccagctc ggcggcgagc tgtttgcgga tcgagccgat
     1921 gcggtcgagg aggtcgccgc gctggttggg gtcgacgagc gggatcaggc cgtagccgat
     1981 ttccagcccg agggtgtcca ccttgagcag gtcctcggtc cgctcctggg tgcggtgttc
     2041 ctgggcgcgg gagcgcttct tcgcctcctc ctcttccacc gcccgcttcg cctgcagttg
     2101 cgtggtgagg tagccgagcc cgccgaccac gccgccgagg gcgaggcagg tgaaggtcgg
     2161 catgccgggg aggaatccga agatg
//
LOCUS       DAOOKS010000339         2182 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_62852_length_2182_cov_2.516690, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000339 DAOOKS010000000
VERSION     DAOOKS010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62852_length_2182_cov_2.516690"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..951)
                     /locus_tag="PK112_06075"
     CDS             complement(<1..951)
                     /locus_tag="PK112_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HPC11649.1"
                     /translation="MRDCISLIIAAILVAAAAAPANCRPSTPTFTENRGQWPDSILFR
                     AEDGPTALWFTTGGVYYQFTRVLGDRHAAGRSALSTDQVARKPEAAELLTVKAALAGA
                     NRAATATGVNPTASRCHFFLGDDPSRWRTEVANFAEIRLTEVYPGIDLVYRGIAADPG
                     SPGGAVEYDFRIAAGADYTQIRVRYEGAEGLAIDPEGGLVIKTAWGDLRESRPVAHQE
                     AHGVRWSVEAAFVITGPREFSFRLGAGYDPSQPAVIDPVLVYGTFLGGTDREQGNGVA
                     VDNAGAIYVAGTTYSADFPTAAPYDGTLGGDADLFVTKLAA"
     gene            complement(1205..>2182)
                     /locus_tag="PK112_06080"
     CDS             complement(1205..>2182)
                     /locus_tag="PK112_06080"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPC11650.1"
                     /translation="PFRKCLSARAVRKILRNYRGAFNASLQIGAYYNLAAALDCPRGL
                     LADGNCALSQRTYATYQTSDAIFRKQREFERAVYRSMDRVFCFSRFLAQSMIDDFGCA
                     PERVKVVGAGINIDETLIRNPERNYGAKTILFSAFDWVHKGGPVLLEAFEKVKREAPE
                     ARLILLGPTLTGLPQGVICHGPLSKDQPQQLQTLGEAFRSASIFVHPTLADAFPNVIR
                     EAMAAGLPCVASDIGSIPEMVTEGQTGYLVPKGNAAQLADRVLRLLANPGLAQAMGEA
                     GYARYRERFTWARVCATIVDELGQVPAERSTGAWPADQTMETALRDSAE"
BASE COUNT          360 a          778 c          673 g          371 t
ORIGIN      
        1 ggccgcaagc ttggttacaa acagatcggc gtccccgccc agcgttccgt cgtatggcgc
       61 cgcggtcgga aaatccgccg agtacgttgt cccggccaca tagatcgccc ccgcgttgtc
      121 cacggccacg ccgttgccct gctcccggtc cgttccgccg agaaacgtcc cgtacacgag
      181 caccgggtcg atgaccgccg gctgtgaggg gtcgtaccct gcccccagac ggaagctgaa
      241 ttcccgcggg ccggtgatga cgaatgccgc ctccaccgac caccgcaccc cgtgcgcctc
      301 ctggtgcgcg accggccgcg actcgcggag atctccccag gcggtcttga tcactagccc
      361 gccctccggg tcgatcgcca gcccctcggc cccctcgtag cgcacccgga tctgcgtata
      421 atccgcaccc gccgcgatcc ggaaatcata ctcgaccgct ccccccggcg accccggatc
      481 agccgcaatc ccccggtaca ccaggtcgat cccagggtac acttcggtca gccgtatctc
      541 cgcaaaattc gcgacctcgg tccgccagcg cgaagggtca tcgccgagga agaaatggca
      601 ccgggaggcg gtcggattca ctcccgtcgc cgtcgccgca cggttcgctc cggccagcgc
      661 cgctttgacc gtcagcagtt ccgccgcctc cggtttgcgg gcgacctggt cggtcgagag
      721 agccgagcgg cccgccgcgt ggcggtcgcc gagaacccgc gtaaactggt agtacacccc
      781 gcccgtcgtg aaccacagag cggtcggtcc gtcctcggcc cgaaagagga tgctgtcggg
      841 ccactgtccg cgattctccg tgaatgtcgg cgttgacggg cggcagttcg ccggcgcggc
      901 ggcggccgcc acaaggatag ctgcaatgat gagagagata caatcccgca tacgctggtg
      961 ttcctgggta tggcgttgtc cgagtattca gaggccccgc gccgctggcg ctgcagcccc
     1021 ggttttccgc gaagaccacc taatatacta gtgtcggcgc ggatagccaa catctttgac
     1081 gcccgcgtct cggcctgcgc gcggcctgcg cctcgcgtta ccccattgcc ggtcgtcgtt
     1141 tccgacctcg cccggcgcgc cgatgacaca gcccggagcg aacgtcgaga gacaaagcgg
     1201 cgggtcattc ggcgctatct cgtaacgccg tctccattgt ctgatccgcc ggccatgccc
     1261 cggtgcttcg ctcggccggc acctgcccca actcgtcgac aatggtggcg cagacgcgtg
     1321 cccaggtgaa ccgctcccgg tacctcgcgt aacctgcctc gcccatcgct tgcgccaaac
     1381 ccggattcgc cagcagccgg agcacacggt ccgcgagctg cgcggcgttc cctttcggca
     1441 ccagatagcc ggtctgtccc tcggtgacca tctccgggat ggagccgatg tcgctcgcga
     1501 cacacggcaa cccggcggcc atcgcctccc gaatcacatt ggggaatgca tcggccagcg
     1561 tggggtgcac gaagatcgag gcggatcgga acgcctcgcc gagtgtctgc aattgctgcg
     1621 gctgatcctt ggacagcggt ccgtggcaga tcaccccttg cggcagcccc gtgagagtcg
     1681 gccccagcag gatgagccgg gcctccggcg cctcccgctt caccttctcg aacgcctcca
     1741 gcagcaccgg tccacccttg tgaacccagt cgaaagcgga gaagaggatg gtcttggcgc
     1801 cgtaatttcg ttcggggttg cggatcagcg tctcgtcgat gtttatcccc gcgccgacca
     1861 ccttcacgcg ctcgggcgcg cagccgaagt catcgatcat cgactgcgcg agaaaccggc
     1921 tgaaacagaa cacccggtcc atcgagcggt agacggcgcg ctcgaattcc ctctgtttgc
     1981 gaaatatggc gtcggatgtc tgatacgtgg cgtacgtgcg ttgcgacaga gcgcagttgc
     2041 cgtcggccag cagcccccgg gggcaatcga gcgcggcggc gagattgtag tacgccccga
     2101 tctggaggct cgcgttgaac gcgccgcggt aatttcggag gatctttctg accgcccgcg
     2161 ccgacaggca cttgcgaaag gg
//
LOCUS       DAOOKS010000340         2177 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_63031_length_2177_cov_4.768615, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000340 DAOOKS010000000
VERSION     DAOOKS010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63031_length_2177_cov_4.768615"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1216)
                     /locus_tag="PK112_06085"
     CDS             complement(<1..1216)
                     /locus_tag="PK112_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11651.1"
                     /translation="MNHKRVFNHFAARTAVGLTVVLSPCLTSRGQQITPNPNPLGNTI
                     TVTNLAENYEVFDNWGIIDIWLSGGLWNWADLRNLDGGTIDNQGTINNGTAADPTGDI
                     DNKGTIVNSGQIIGFGTIANSGTVENEDGAIDNSGTLDNLGAIHNSSSSFLYNRYRGT
                     IHNGTAADSAADIDNEGIIVNDGQIIGSGTLANSGTLENNGGIVNDGALDSSGTIDNS
                     GTIDNFGVIHNRSSGFLYNRYWGTIHNGTAADSAADIDNEGTIVNDGRIIGFGTLANS
                     GTLENNGGIVNDGALDSSGTIDNSGTIGNLGAIDNRSSGLLYNRDLGIINNGTAAASA
                     ADIDNEGTIVNRGQIIGFGTIDSSGTLDNLGAISNRSSGFLYNRNSGVINNGTAADSG
                     GDIDNRGTIVNSG"
BASE COUNT          433 a          562 c          698 g          484 t
ORIGIN      
        1 ctccgctgtt tacgatggtc cccctattgt cgatgtcgcc gccagagtca gcggcagtgc
       61 cattgttgat gacacccgaa ttccggttgt agaggaaccc cgaactcctg ttggagatag
      121 cgccgagatt gtcgagggtg cccgagctgt cgatggtgcc aaagccgatg atctgcccgc
      181 ggttcacgat ggtcccctca ttgtcgatgt cggcggcaga ggcagcggca gtaccattgt
      241 tgatgatacc caaatcccgg ttgtagagta accccgagct cctgttgtcg atagcgccga
      301 ggttgccgat ggtaccagag ttgtcgatgg tgccgctgct gtcgagagcg ccatcgttca
      361 ctatgccgcc attgttttcg agagttcccg agttcgcgag ggtgccaaaa ccgatgatcc
      421 gcccatcgtt tacgatggtc ccctcattgt cgatgtcggc ggcagaatca gctgcagtgc
      481 cattatggat ggtaccccaa taccggttgt agaggaagcc cgagctcctg ttgtggatga
      541 cgccaaaatt gtcgatggta ccagagttgt cgatggtgcc gctgctgtcg agagcgccat
      601 cgttcactat gccgccattg ttttcgagag ttcccgagtt cgcgagggtg ccagaaccga
      661 tgatctgccc gtcatttacg atgatcccct cattgtcgat gtcggcggca gaatcagcgg
      721 cagtaccatt atggatggta ccccgatacc ggttgtagag gaagctcgaa ctgctgttgt
      781 ggatggcgcc gagattgtcg agggtgccac tgttatcaat ggcgccgtcc tcgttctcga
      841 cggttcccga gtttgcgatc gtgccaaaac cgatgatctg cccgctgttt acgatggtcc
      901 ccttattgtc gatgtcgccg gtagggtccg cggcagtgcc attgttgatg gtgccctggt
      961 tgtcgatggt gcctccgtcc aagtttctga gatcggccca attccagagg cccccgctga
     1021 gccagatgtc aatgatgccc cagttgtcga acacctcata gttctcagcg agatttgtga
     1081 cggtaatcgt attgccgagg ggattcggat tcggagtgat ttgctgcccc cttgacgtca
     1141 ggcacgggga cagcacaaca gtcaaaccaa ccgccgttct cgccgcaaaa tgattgaaaa
     1201 cgcgtttatg gttcatagta tcctctcctc cctgggggaa tcaaagctgt cggataccgt
     1261 cttcctccgt ttcagattgc cgcctgcggc ccgaatccaa cgagttcgtc acagctttgg
     1321 gccagatgta cctccacgag cctcagacta ttatggcgga agtcgaccgc aagggcaagc
     1381 aggaaagtgg ctacggacaa cattttcccg agaacagacg gcggaagacg gcgggaccag
     1441 gtgcaccggt caagcagcgg tcggaccgct ctggagcggg caagaatcgg cctcgaagcg
     1501 ccctcaggaa aggggcaggt gtgggaccgc ggtgaaaggg tgccagtcgt ttcaacgcga
     1561 tcgccggtcg aaggcagaga ttgggggcca tcaactcgtg catgggtagg ctgcgaatgg
     1621 cagaagcccc agagatatgt gctcgaaggt gtcaacgggg ctcggcgagt tcacggtgcc
     1681 gagagtggag gggcaggttc cgcagccgct ccgtgcgtcg ctttccctgg ggaactctgg
     1741 gctgctgtca gggatgtggg aatcatgaca gactatgtga aggcggggag gcagaacagg
     1801 ggcagagggc agagaacgct ggcttggaat caccgcaact ggtgagaggt gcacaatgcc
     1861 tactggatga gacggagtat tcggacgggt tgtcctattg cctcgccaaa aggtggcttt
     1921 tgtgtgcttg acaggggttt gggggaggga agactcgacg gaaatcgcag tgtatagtga
     1981 cattttagcg acatgtaccg cgcgagactt gaagtgtgtc gctgtcgttc acacgtgtgc
     2041 catctaccaa aatggtctat tgcggcgaag gtggcggttg gaggcggacg tcctgggatt
     2101 gggtaaaggg gccgggaggg ctcacggccc ccgcggtcgt gacgtgggag atgcgggcta
     2161 cggcggttct tccgttg
//
LOCUS       DAOOKS010000341         2177 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_63041_length_2177_cov_3.403864, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000341 DAOOKS010000000
VERSION     DAOOKS010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63041_length_2177_cov_3.403864"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /gene="metK"
                     /locus_tag="PK112_06090"
     CDS             <1..794
                     /gene="metK"
                     /locus_tag="PK112_06090"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HPC11652.1"
                     /translation="SRETAELMPMPIQFAHKLCRRLAEVRKKAILPYLGPDGKAQVTI
                     EYEEGRPRRCEAIVVSTQHRESILDATGKKITEQARAEIIQNVVAPVIPADMIDARTK
                     FYVNPTGRFVIGGPRSDTGMTGRKIIVDTYGGMAAHGGGAFSGKDPTKVDRSASYMAR
                     YIAKNVVASGIAEKCTLQVAYAIGVAEPVSLTVFADGTAKIPERRIAELIRKHFDLTP
                     RGIIEKLNLLRPIYSATAAYGHFGREESEFTWERTDMAAALAKDA"
     gene            798..2057
                     /gene="ahcY"
                     /locus_tag="PK112_06095"
     CDS             798..2057
                     /gene="ahcY"
                     /locus_tag="PK112_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HPC11653.1"
                     /translation="MGKRTDIHNPRLAGPGRDRIEWAEQRMPVLRLIRERFRKEQPLK
                     GLRLACCLHVTTETANLVRTLQAGGATVALCASNPLSTQDDTAASLVKDFGIAVYAIH
                     GEDTKTYYRHIHQALDGRPHITMDDGADLVSTLHSERQELIGNILAGTEETTTGVIRL
                     RAMAQEKRLLFPVIAVNDSRTKHFFDNRYGTGQSTIDGVLRATNMLIAGSTVVIAGYG
                     WCGRGLAMRAKGMGAQVIVTEVDALKALEATMDGYTVLPMAQAAPKGDLFITVTGDLH
                     VIRLEHFRRMKDGAIVCNSGHFNVELDLAALAKAARRKRRVRPNVDEYVLDRRRIYVL
                     GEGRLINLAAAEGHPAMVMDMSFANQALAAEYVVHNHALLDRCVYTVPEAIDRQIAAL
                     KLKSMGIALDRLTPEQRKYLASWDMGT"
BASE COUNT          393 a          727 c          741 g          316 t
ORIGIN      
        1 ccagccgcga gacggcggag ctcatgccga tgccaatcca gttcgcgcac aagctgtgcc
       61 gccgcctcgc cgaggtgcgc aaaaaagcga tcctccccta cctcggcccc gacggcaagg
      121 cccaggtgac gatcgagtac gaagagggcc ggccccggcg gtgcgaggcg atcgtcgtct
      181 ccacccagca ccgggagtcg attctcgacg cgacggggaa gaagatcacc gagcaggcgc
      241 gcgcggaaat aatccagaac gtcgtcgcgc ccgtcattcc cgccgacatg atcgacgcgc
      301 ggacgaagtt ctacgtcaat ccgaccggca gattcgtgat cggcgggccg cgctccgaca
      361 cggggatgac cgggcggaaa atcatcgtcg acacctacgg cggcatggcg gcgcacggcg
      421 gcggagcgtt ctcgggcaag gatccgacga aggttgaccg ctcggccagc tacatggccc
      481 gctacatcgc caagaacgtg gtggcctccg ggatcgccga gaagtgcacg ctgcaggtgg
      541 cctacgccat cggcgtcgcc gagccggtgt cgctgacagt gttcgccgac gggacggcga
      601 agattccgga acggcgcatc gccgaactga tccgcaagca cttcgacctc accccccggg
      661 gaatcatcga gaagctcaat ctgctgcgtc cgatctattc ggccaccgcg gcctacgggc
      721 atttcggccg cgaggagagc gagttcacgt gggagcggac ggacatggcg gccgccctgg
      781 cgaaggatgc gtgagcgatg ggcaagcgga cggacattca taatcccagg ctggccggcc
      841 cggggcgcga ccgcatcgag tgggccgagc agcgcatgcc ggtgctccgg ctgatccggg
      901 aacggttccg caaggagcag ccgctgaagg gtctccgcct ggcctgctgc ctgcacgtga
      961 cgaccgagac ggcgaacctc gtgcgcacgc ttcaggcggg cggcgcgacg gtcgccctgt
     1021 gcgcctcgaa ccccctttcg acccaggatg acacggcggc ctcgctcgtg aaagatttcg
     1081 gcatcgccgt ctacgccatc cacggcgagg acaccaagac ctactaccgg cacattcacc
     1141 aggcgctcga cggccggccg cacatcacga tggacgacgg ggcggacctg gtgtcgacgc
     1201 ttcacagcga gcgccaggaa ctcatcggca acatcctcgc cgggaccgag gagaccacga
     1261 ccggcgtcat ccggctgcgg gcgatggccc aggagaagcg gctcctgttc ccggtgatcg
     1321 cggtcaacga ttcccggacc aaacacttct tcgacaaccg ctacggtacg ggccagtcga
     1381 cgatcgacgg cgtcctgcgc gccaccaaca tgctcatcgc cgggtccacc gtggtcatcg
     1441 ccggctacgg ctggtgcggc cgggggctgg cgatgcgggc gaaggggatg ggcgcgcagg
     1501 tgattgtgac cgaggtcgac gcgctcaagg cgctcgaggc gaccatggac ggctacacgg
     1561 tgctgccgat ggcgcaggcg gcgcccaagg gggacctctt tatcaccgtg accggcgacc
     1621 tgcacgtgat ccggctcgag cactttcgcc ggatgaagga cggcgcgatc gtgtgcaact
     1681 cggggcactt caatgtcgag ctcgacctgg cggcgctcgc gaaggcggcc aggaggaaac
     1741 gccgggtgcg gccgaatgtc gacgagtacg tgctcgaccg gcggcgcatt tatgtgctcg
     1801 gcgaggggcg gctgatcaac ctggcggcgg ccgaggggca ccccgcgatg gtgatggaca
     1861 tgtcgtttgc caaccaggcc ctggccgccg agtatgtggt acacaatcac gccctgctcg
     1921 accggtgcgt ctacactgtg cccgaggcga tcgaccggca gattgcggcc ctgaagctga
     1981 aatcgatggg catcgcgctc gaccggctta cccccgaaca gcgcaagtac ctggccagct
     2041 gggacatggg gacgtagggc cgccgcccgc cgagagtctc cgcagttgcc gctccatccc
     2101 gccttctgcc ggccgccgtc tgcccacgct cgccggggaa agcaatcggc ggcatggtct
     2161 ttggcgacgg ttccatc
//
LOCUS       DAOOKS010000342         2172 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_63229_length_2172_cov_3.605574, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000342 DAOOKS010000000
VERSION     DAOOKS010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63229_length_2172_cov_3.605574"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..839
                     /locus_tag="PK112_06100"
     CDS             <1..839
                     /locus_tag="PK112_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11654.1"
                     /translation="SDRLLQASCRAALAGLYRETGQGQKEIAQLEQLSDTASQAYPAV
                     RARIAEIVGFQFRDFTRAAAMYDTLLARLGPSDTAYYPDLLFRKALIRMEQKRYGDAR
                     QILVALKRDHPRYWAMTPPAQLAMARTFELEDNWDRAEVEYHALIEGYRGSDEAMGAF
                     LYVARRYEQLGRKDQADRWYRDAREYYAQVARYSQEGLMEARALSFQAELDSRLGNWA
                     EAAGTLEDLYRKYGRHEIGRQALVRAAAIRRERLGQQQVADSLLTALKAGLTEIEPTS
                     EL"
     gene            901..1497
                     /locus_tag="PK112_06105"
     CDS             901..1497
                     /locus_tag="PK112_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11655.1"
                     /translation="MHITRLLTLSALGILLLASLGAAEEPQKPAAKPDNFGILDTVYA
                     EVSRIDPQNWSMTISATNDENVEGLAVPLKLSSGTVRIVADSAVYTGGRVDHFAYRGF
                     RPDTMQQCVTLGMVANMGPSTNVLAPGKGRLVTVFVSSIEDQPVENLVVDTTTTSPSN
                     SLMLVAQFEEIKNRLGEEAIRADKRLEIYPAFVVVKSK"
BASE COUNT          391 a          672 c          720 g          389 t
ORIGIN      
        1 cttccgaccg gctcctgcag gcgtcctgcc gggcggccct ggccggattg taccgcgaaa
       61 ccgggcaggg gcagaaagag atcgcgcagt tggagcagct ctcggacacg gcctcgcagg
      121 cgtatccggc agtccgcgcc cggatagccg agatcgtcgg ttttcagttc cgcgatttca
      181 cccgcgcggc cgccatgtac gacactctcc tggcccggct ggggccctcc gacacggcct
      241 actacccgga tttgctcttc cgcaaggcgc tgatccggat ggagcaaaag cgctacggcg
      301 atgcccgcca gatcctggtg gccctcaagc gtgatcatcc ccgctactgg gccatgactc
      361 ccccggccca gctggctatg gcccgcacct tcgaactgga agacaactgg gatcgggccg
      421 aggtcgagta ccacgctctg atcgaggggt accggggttc cgacgaggcg atgggggcgt
      481 ttctctacgt ggcgcggcgc tacgagcagt tggggcgcaa ggaccaggcc gaccggtggt
      541 atcgggatgc ccgggagtat tacgcccagg tcgctcgcta cagccaggaa ggcctgatgg
      601 aggcccgggc cctgagtttt caggctgagt tggacagccg cctgggcaac tgggcggagg
      661 cggccgggac gttggaggac ttgtaccgga aatatgggcg gcatgaaatc ggccgccagg
      721 cgctcgtccg ggccgccgcg atccgccggg aacgccttgg gcaacagcag gttgccgatt
      781 cgctcctgac cgcgctgaaa gccggcctga ctgagatcga accgacctct gaattgtaaa
      841 agagaatagg ccgcccgccg cagtggcgga cggtccgacc gaattcagcc tggaggaaag
      901 atgcacatca ccagattgtt aacgctgtcc gcccttggga ttcttctgct ggcgtcgctc
      961 ggcgccgccg aggaaccgca aaaaccggcc gccaagccgg ataatttcgg catcctcgat
     1021 accgtgtacg ccgaggtctc ccggatcgat ccgcagaatt ggtcgatgac aatcagcgcc
     1081 accaacgacg agaatgttga gggcctcgcc gtgccgctga aactctcgag cgggacggtg
     1141 cggatcgtgg ccgactccgc cgtctacacc ggcggccggg tcgatcattt cgcctaccgc
     1201 ggcttccgcc cggatacgat gcagcagtgc gtgacgctcg gcatggtcgc gaacatgggg
     1261 ccgagcacca acgtgctggc gcccggcaaa ggccggctcg tgactgtctt cgtctcctcg
     1321 atcgaagacc aaccggtcga gaacctggtt gtggacacca cgaccaccag cccgagcaac
     1381 tcgctcatgc tggtggcgca gttcgaggaa atcaagaacc gcctgggtga agaggcgatc
     1441 cgcgccgaca agcggctcga gatctacccg gcatttgtgg tggtcaagtc gaagtagtcg
     1501 cgcttgcgaa gcttttctct cgggagcccg gtcatccgac cgggcttctc gtttatatgg
     1561 ggggcgctcg gcagggccgc gtccgattcc gccggatacg cccgcgaacg ggatttcgca
     1621 ggccgggacc cttgtggtcc tgacgggcct gcttctatcg atggcgagaa tgtcaggatc
     1681 acaaaaatcc tgacctacgg gagattttga ggatcgtccc gtggaattcg ggtcggtttg
     1741 cagatctcgc agcatagccg caccgagccg ggtagttgct ttggtcaacg gtgcccgcgc
     1801 cgcagacatt gcgacgcgct ttgtcaagcg gaacgaaacc gcttgcgcat ccgtcccgta
     1861 tgaaatctca aaaggaaccg atccccggag atcctggtcc tgttccgccc acggtgcgga
     1921 ggcagcggcc aggcggacgg ttgcgtacaa ccaggaacat cttgcacgaa tgggaaaggg
     1981 gaaggtgtat gaatgtgcgc gcagtgaacc ggggtttgct tgtgtctctg tcggcggctg
     2041 ttttgctcgg gcttgcgccc caggctcgcg gttcgaccgc gatgctgccg gcctcggatt
     2101 tcacgccggt gtaccggccg tcgatcacgc tgcgcccggc ggctgggagg atcgaggtcg
     2161 acggggacct gg
//
LOCUS       DAOOKS010000343         2170 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_63322_length_2170_cov_3.033097, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000343 DAOOKS010000000
VERSION     DAOOKS010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63322_length_2170_cov_3.033097"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /gene="lexA"
                     /locus_tag="PK112_06110"
     CDS             <1..448
                     /gene="lexA"
                     /locus_tag="PK112_06110"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HPC11656.1"
                     /translation="LKKEAQISRGLAPTRTLALRVGRVPLVGTVPAGRPIDAVENVEG
                     EIAVDESFLPKEDSFSLRVAGNSMQGAGILDGDIVLVKKQRVAQKGDIVVAIIGGEAT
                     VKRYFPEGNRVRLQPENDDFEPIFVDRKSDEFRIAGKVIGLLRRLK"
     gene            complement(537..1154)
                     /locus_tag="PK112_06115"
     CDS             complement(537..1154)
                     /locus_tag="PK112_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HPC11657.1"
                     /translation="MLKKAALASSVVLAAGAGVSAAQWEIDRAHSSIGFSVRHMVVSK
                     VPGKFTDFDGNLEMAQAADGKLDLASAKVSMTAKAASINTDNDKRDEHLRSADFFDAA
                     TYPELTFVSKKVIPGEGDKFKLIGDLTIRGVTKEVTFDGTLNGVVTDFMGNTRAGFSA
                     AATINRQDFGVSWSKLLDNGGLVAGDNVDIIIEIEAVLPKPESKG"
     gene            complement(1257..1841)
                     /gene="rlmB"
                     /locus_tag="PK112_06120"
     CDS             complement(1257..1841)
                     /gene="rlmB"
                     /locus_tag="PK112_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HPC11658.1"
                     /translation="MPRKPDSPRRHRHTDYSALPASLDEDSLLRRLGALPGPALVLVL
                     DSITDPHNLGACLRSAEGAGAHAVVVPKDRSVGLTDTVRHVAVGAAEHIPFAMVTNLA
                     RTLDQLKEAGLWVVGTTHETERVIYDLDLTGPVVFVMGSEGKGLRRLVAEKCDFLARI
                     PMAGRIENLNVSVAAGVCLFEAVRQRRAGTPGRT"
     gene            complement(1858..>2170)
                     /locus_tag="PK112_06125"
     CDS             complement(1858..>2170)
                     /locus_tag="PK112_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11659.1"
                     /translation="RHLQGRLLFAPHAGERHLRELLADGGAVMRTPITPEFDLKQFRE
                     IYGESAVPLFIITEDRSLKICTVQDPPAPKPGERLIALVSTGRAGGRVGDAGDVHPAR
                     L"
BASE COUNT          387 a          628 c          746 g          409 t
ORIGIN      
        1 cctgaagaag gaggcgcaga tttcgcgggg gctggcaccg acgcgcacgc tggcgctccg
       61 ggtggggcgg gttccgctgg tggggacggt tccggccggc cgcccgatcg acgcggtcga
      121 gaatgtcgag ggtgagatcg ccgtcgacga gtcgtttctt ccgaaagagg actcgttttc
      181 gctgcgggtg gccgggaatt cgatgcaggg cgcggggatt ctcgacggcg acatcgtgct
      241 ggtgaaaaag cagcgggtgg cgcagaaggg ggatatcgtg gtggcgatta tcggcggcga
      301 ggcaaccgtc aagcgctatt tcccggaggg gaaccgggta cggctgcagc cggaaaacga
      361 tgactttgaa ccgatttttg tggatcgcaa gtccgacgag ttccggatcg cgggtaaagt
      421 catcgggttg ttgcggcggc tgaagtagct tttcccggtg cggcgcctac ggattttggg
      481 acacaagaaa gcccgcacgg acgcgggctt ttccgtatca aagtcggccg cttggctcag
      541 cctttggatt ccggcttggg caggaccgcc tcgatctcaa tgatgatgtc gacgttgtcg
      601 ccggcaacga gtccgccgtt gtcgaggagt ttactccacg agacgccgaa atcctggcgg
      661 ttgatggtcg cggcggcgga gaaaccggcc cgcgtgttgc ccatgaagtc ggtcaccaca
      721 ccattgaggg tgccgtcgaa cgtcacttct ttagtcacgc cccggatggt caggtcgccg
      781 atgagcttga acttatcccc ctcacccggg atcaccttct ttgacacgaa agtcagttcg
      841 gggtaggtgg cggcgtcgaa gaaatcggcg ctgcgcaggt gttcatcgcg tttgtcgttg
      901 tcggtgttga tggaggccgc cttggcggtc atggacactt tggcgctggc caggtcgagc
      961 ttgccgtcgg ccgcctgggc catctcgagg ttgccatcga agtcggtgaa ttttccgggg
     1021 actttcgaga cgaccatatg gcgcacggag aagccgatgg acgagtgagc acggtcgatt
     1081 tcccattgcg ccgcggatac gccggcgccg gcggccagca cgaccgatga ggcaagagca
     1141 gccttcttca gcatgtcaaa atctcctctg tcagtgtttc cagttcgttt gccgaattat
     1201 aacagcggat cgggccgacg gttccgggcc cttgaagtga ttgatccgcc catacatcat
     1261 gtgcggccgg gggttccggc gcggcgctgg cgcacggcct cgaacaggca gacgccggcg
     1321 gcaaccgaaa cattcaaatt ttcgatccgg ccggccatgg gaatacgcgc gaggaaatcg
     1381 catttttcgg ccaccaggcg gcgcaaaccc ttcccttcgc tgcccattac gaagaccacc
     1441 ggaccggtga ggtcgaggtc gtagatcacc cgctcggttt cgtgggtcgt gccgactacc
     1501 cagaggccgg cctccttcaa ttgatcgagg gtccgcgcga ggttggtgac catggcgaag
     1561 gggatgtgtt cggcggcgcc gactgcgacg tggcgaacgg tgtcggtgag accgacggag
     1621 cggtctttgg ggacaacgac cgcatgtgcg ccggcaccct cggccgaccg aaggcaggcc
     1681 ccgaggttat gcggatcggt gatcgagtca agaaccagca ccagggccgg acccggcagc
     1741 gcgcccagcc ggcggagcag actgtcctca tccagcgacg cgggcaaggc ggagtaatcg
     1801 gtgtgccgat gacggcgggg ggaatcgggt ttgcgcggca tggcctcatt tctcctctca
     1861 caaccgtgcg gggtgcacgt cgcccgcatc gccgacccgg ccgcccgcgc ggccggtgga
     1921 cacgagcgcg atcagcctct cccccggttt gggcgcgggc ggatcctgca cggtacagat
     1981 tttcagagaa cgatcttcag ttatgataaa aagcggcacg gccgactccc catagatttc
     2041 ccggaactgc ttcaggtcga attccggcgt gatgggagtc cgcatcacgg ctccgccgtc
     2101 ggccagcagt tcgcggaggt gtcgctcgcc cgcgtgagga gcaaaaagga gccggccctg
     2161 gagatgccgg
//
LOCUS       DAOOKS010000344         2166 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_63499_length_2166_cov_3.226433, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000344 DAOOKS010000000
VERSION     DAOOKS010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63499_length_2166_cov_3.226433"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="PK112_06130"
     CDS             <1..328
                     /locus_tag="PK112_06130"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HPC11660.1"
                     /translation="PADLFFSPIYAVQKLMKKMNVAISHWDLIEANEAFAAQALADGQ
                     GLGWDWDRVNVHGGAVALGHPIGASGTRILTTLIYALKDRGKKHGLATLCLGGGNAVA
                     CAIELV"
     gene            349..1212
                     /locus_tag="PK112_06135"
     CDS             349..1212
                     /locus_tag="PK112_06135"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010899925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HPC11661.1"
                     /translation="MKAEDIKTVTVIGSGTMGNGIAQVFAQTGYAVHLIDIEQSFLDR
                     AVKTISGSFDRLIKKEKISEADKSAALGRIKTSTDLGAAKVAQLVIEAATEDLKVKLD
                     IFGRLEGICPPETIFASNTSSLPITQLAAATKRPPQFIGMHFMNPVPMMQLVEVIRGI
                     ATSDDTYRTIEGLARKLGKTPVEVNDYPGFIANRILLPMINEAVYALMEGVGTKEAID
                     DVMKLGMAHPMGPLTLADFIGLDVCLAILEVLHAGLGDPKYRPCPLLRKMVQAGYLGR
                     KTGRGFYDYSK"
     gene            1224..>2166
                     /locus_tag="PK112_06140"
     CDS             1224..>2166
                     /locus_tag="PK112_06140"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPC11662.1"
                     /translation="MDFKLSEEDLELKQMAHELAVKRIYPIAEEMDEKGETPAELIRE
                     CAELGYFGFTVPEAYGGLGLSATSFMGVLEEICGASAGFGIMLSVHNSLCCEIIKLFG
                     SETLKQKYLPAMAAGECIGAYCITEPDAGTDVASLKTTAADGGDGWVLNGTKSYVTNG
                     SFAGLFVVFAKTDPDAGHKGISCFLVPAGTPGLMIGKAERKCGIKASDTREVSFSDVR
                     IPKDHILGAPGQGFRMAVTILNSGRVGVAFQSIGIAQAALNEALKYAKERKQFNQPIA
                     NFQAIQFKLADMALKIDAGRLLAYRAAQLKDAGEPCHR"
BASE COUNT          418 a          638 c          752 g          358 t
ORIGIN      
        1 gccggcggat ttgttcttct ccccgatcta cgcggtgcag aagctcatga agaagatgaa
       61 cgtcgccatc tcccactggg acctgatcga ggcgaacgag gcgttcgccg cccaggcgct
      121 ggccgacggc caggggttgg gctgggactg ggaccgggtg aacgtgcacg gcggcgcagt
      181 ggctctcggc cacccgatcg gggcctccgg cacccgcatc ctgaccacgc tcatctatgc
      241 gctcaaggac cgcggcaaaa agcacggcct ggcgacgctg tgtctcggcg gcggcaacgc
      301 cgtcgcctgt gccattgaac tggtgtaacc caacgaggat gaggcatcat gaaagcggaa
      361 gatatcaaga cggtgacggt gatcgggagc ggcacgatgg gcaacggcat cgcccaggtg
      421 ttcgcccaga ccgggtacgc ggtgcacctg atcgacatcg agcagtcctt cctggaccgg
      481 gcggtcaaga ccatcagcgg gagtttcgac cggctgatca agaaggaaaa gatcagcgag
      541 gccgacaaat cggccgctct cggccggatc aaaacctcga ccgatctcgg cgccgccaag
      601 gtggcccagt tggtgatcga ggcggcgacc gaagacctga aagtgaagct tgacattttc
      661 ggccggctcg aagggatctg cccgccggag acgattttcg cctcgaacac gtcctcgctg
      721 ccgatcacgc agctggccgc ggcgaccaag cggccgccgc agttcatcgg catgcacttc
      781 atgaacccgg tgccgatgat gcagttggtc gaggtgatcc gggggatcgc gaccagcgac
      841 gacacctatc ggacgatcga ggggctggcg cggaagctgg ggaagacgcc ggtcgaagtg
      901 aacgattacc ccgggtttat cgccaaccgc atcctcctgc cgatgatcaa cgaggcggtg
      961 tacgcgctca tggagggggt ggggacgaag gaggccatcg acgatgtgat gaagctcggg
     1021 atggcgcatc cgatgggtcc gctgacgctg gcggatttta tcgggctcga cgtctgcctg
     1081 gcgattctcg aggtgctgca cgccgggttg ggcgacccca agtaccgtcc ctgcccgctg
     1141 ctgcgcaaga tggtgcaggc cggctacctc ggccgcaaga ccggccgcgg gttctacgac
     1201 tacagtaagt gaggagcctt gcgatggatt tcaagttgtc cgaagaggac ctggaactca
     1261 aacagatggc gcacgaactg gccgtcaagc gcatctaccc gatcgccgag gagatggatg
     1321 agaaggggga gacgccggcg gagttgatcc gggagtgcgc cgaactgggg tatttcgggt
     1381 tcacggtgcc ggaagcgtac ggcgggctgg ggctgagcgc gacctcgttc atgggcgtgc
     1441 tcgaggaaat ctgcggggcg agcgccggct tcggcatcat gctctcggtc cacaattccc
     1501 tctgctgcga gatcatcaag ctgttcggat cggagacgct caagcagaaa tacctccccg
     1561 ccatggcggc gggggagtgc atcggcgcct actgtatcac cgagccggac gccgggacgg
     1621 acgtggcctc gctgaagacg acggcggcgg acggcggcga cggctgggtg ctcaacggca
     1681 cgaagagcta tgtgactaac ggatccttcg ccgggctctt cgtcgtgttc gccaagaccg
     1741 atccggatgc cgggcacaag gggatttcgt gtttcctcgt gccggcgggc accccgggcc
     1801 tgatgatcgg gaaggcggag cggaagtgcg ggatcaaagc ttcggacacg cgcgaggtca
     1861 gtttttccga cgtgcggata ccgaaagacc acatcctcgg cgcgccgggg caggggttcc
     1921 ggatggcggt gacgattctg aattcggggc gggtcggggt ggctttccag tcgatcggga
     1981 tcgcccaggc ggctctcaat gaggcgctga agtacgccaa ggagcgcaag cagttcaacc
     2041 aaccgatcgc caatttccag gcgatccagt tcaaactggc cgacatggcg ctgaagatcg
     2101 atgccgggcg gctcctcgcc taccgggccg cgcagctcaa ggatgccgga gagccctgcc
     2161 accggg
//
LOCUS       DAOOKS010000345         2160 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_63775_length_2160_cov_3.019002, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000345 DAOOKS010000000
VERSION     DAOOKS010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63775_length_2160_cov_3.019002"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..632)
                     /locus_tag="PK112_06145"
     CDS             complement(81..632)
                     /locus_tag="PK112_06145"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HPC11663.1"
                     /translation="MRKIHVTLFIPAAVLLWAVGGRAAETVKVGDPAPAFTLPFATRD
                     SVDLKGLSSAELAGKRYVLAFYPADWSSGCTAEMCMLRDEFAGFEKLGVIVLPVSGDY
                     VFSHREWAQYHNLPFRLLSDPTREFGRKMGVYREDTGMFQRSVFVVGPTGTLEYVDYE
                     YSVKDRDDFEALRDALGSAESRE"
     gene            complement(741..1052)
                     /locus_tag="PK112_06150"
     CDS             complement(741..1052)
                     /locus_tag="PK112_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11664.1"
                     /translation="MADSGLLCWNCGRATGVEGHPTRNDSCPHCLADLRACRGCRHFD
                     PYARAQCRETIEAPVRDKEKSNFCDFFQVRDAIKRPGGIRASTDSKEERKKRFDDLFN
                     D"
     gene            complement(1131..2141)
                     /locus_tag="PK112_06155"
     CDS             complement(1131..2141)
                     /locus_tag="PK112_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HPC11665.1"
                     /translation="MVHKVTLIPGDGIGPEITEAAVRVIEAAGVEIAWDRQEAGIPAV
                     QTHGTSVPDQLLDSIHANKVALKGPITTLVGKGFRSANVTLRQRLDLYVNLRPVRTIG
                     GVPSRYSKVNLVIVRENTEDLYSGIENMITPGVTQAIKVVTERASERVARWAFNYARK
                     MGRKKITFVHKANIMKLSDGLFLKVFEDVAKDYPDIEAEDRIVDALCMRLVMDPTQFD
                     MLLMGNLFGDIVSDLAAGLVGGLGVVPGANIGDEYAVFEAVHGSAPDIAGKNLANPTA
                     LIFSALLMLRHLGEDAAADRIWKSMVVTLAEGHKLTRDLGGTCSTTEFTDELVREIKS
                     RT"
BASE COUNT          359 a          773 c          635 g          393 t
ORIGIN      
        1 gccatgaagg tcctgaccta cagaacctgt ttcgcggggg taggcgagcc cgtgttgcgg
       61 ccgccccggc cgaacccctc ttattcgcgc gattcggccg atcccagggc gtcccgcaaa
      121 gcctcgaaat cgtcccggtc cttcaccgag tactcgtaat cgacatactc cagcgtcccc
      181 gtgggcccga ccacaaacac ggaccgctga aacatcccgg tgtcctcccg gtacacgccc
      241 atcttcctgc cgaactcgcg cgtcggatcg gacagaagcc ggaagggcag attgtggtac
      301 tgcgcccact cccgatggct gaacacgtag tcccccgaca ccgggaggac gatcactccc
      361 aatttctcga acccggcgaa ctcatcccgc agcatgcaca tctcggccgt gcacccgctc
      421 gaccagtcgg ccggatagaa cgccagcaca taccgtttcc cggccagttc cgccgagctg
      481 agccctttca ggtcaacgga atctctggtc gcaaacggga gcgtgaacgc cggcgccggg
      541 tcgccgactt tgactgtctc agcggcacgg ccgcccaccg cccagagaag caccgccgcc
      601 gggataaaca gagtgacatg gattttgcgc atcgccgttt ctctcgtgat tggtttttcg
      661 gctcagaacg gagcggccgg cccgggggtt ccccggtcgc gccggaacgg gagtgcggcg
      721 tccggacccg cgtccggagg ctagtcgttg aagaggtcgt cgaaccgctt cttccgctct
      781 tccttgctgt ccgtcgaggc ccgaatcccc cccggccgtt tgatcgcatc gcgaacctga
      841 aagaagtcgc agaagttgct tttttccttg tcccgcaccg gagcctcgat cgtctcgcgg
      901 cactgggcgc gggcgtaggg gtcgaaatgc cggcacccgc ggcatgcgcg caggtcggcc
      961 aggcagtgag ggcaactgtc gttccgcgtc ggatgtccct ccacgcccgt tgcccgtccg
     1021 cagttccagc acaacagacc gctgtcagcc atgatcaacc tcctccggca gacgccgccg
     1081 ccgcggccgg ttcccgcgcg cggactttca tgaatccgcg ccgcggcggc tcacgtgcga
     1141 ctcttgatct ctctcaccag ctcgtcggta aactcggtcg tcgagcacgt cccgccgagg
     1201 tcccgcgtga gcttgtgccc ctccgcgagc gtcaccacca tcgatttcca tatccggtcg
     1261 gccgccgcat cctcgccgag atgccgcagc atgagcaggg cggagaagat gagggcggtc
     1321 gggttcgcga ggttcttgcc cgcgatatcc ggagccgagc cgtgcaccgc ctcgaacacc
     1381 gcgtactcgt cgccgatgtt cgcccccggc accaccccga gcccgccgac cagcccggcc
     1441 gcgagatcgg agacgatatc gccgaacagg ttccccatga gcagcatgtc gaactgcgtc
     1501 ggatccatga ccagccgcat gcacagggca tcgacaatgc ggtcctccgc ctcaatgtcg
     1561 ggatagtcct tggcgacgtc ctcgaacacc ttgaggaaaa gaccgtcgga gagtttcatg
     1621 atgttcgcct tgtgcacgaa cgtgatcttc ttccggccca ttttgcgggc gtagttgaag
     1681 gcccaccggg ccacccgctc cgacgcccgc tcggtcacca ccttgatcgc ctgcgtcacc
     1741 cccggcgtga tcatgttttc gatccccgag tacaagtcct cggtgttctc ccgcacgatc
     1801 acgaggttga ccttgctgta ccggctcggc acgccgccga tggtccgcac cgggcgcagg
     1861 ttcacgtaca aatcaagccg ctggcggagc gtcacgttcg ccgaccggaa ccccttcccc
     1921 accagcgtcg tgatcggccc cttgagcgcc actttgttgg cgtggatcga gtcgagcagt
     1981 tggtcgggca ccgacgtgcc gtgggtctgg accgccggaa tgccggcctc ctggcggtcc
     2041 caggcgattt ctacccccgc cgcctcgatg acccggacgg ccgcctcggt gatctccggg
     2101 ccgatcccat cgccgggaat gagcgtcact ttgtgcacca tgctgatgta ccggcatcct
//
LOCUS       DAOOKS010000346         2155 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_63963_length_2155_cov_3.600476, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000346 DAOOKS010000000
VERSION     DAOOKS010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63963_length_2155_cov_3.600476"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..900
                     /locus_tag="PK112_06160"
     CDS             <1..900
                     /locus_tag="PK112_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPC11666.1"
                     /translation="RVIDQLLISLLASGHCLLVGVPGLAKTLLISTLARVLDLRFNRI
                     QFTPDLMPSDITGTEIIEEDQATGRRRFRFVKGPVFAHIILADEINRTPPKTQAALLQ
                     AMQEHEVTAAGETYKLDEPFFVLATQNPIEQEGTYPLPEAQLDRFMFNVFVDYPSERE
                     EREIVRSTTAVLDYQLNKILSPQEIIGFQQLVRRVPVSDHLVEYAVKLARKTRPHEPD
                     APDFVRNWVNWGAGPRASQYLILAAKTRAILDGRPTPGPEDVRFAAYPVLRHRIVTSF
                     NAEADGVDTMEIIKRLLETVKEQ"
     gene            1621..>2155
                     /locus_tag="PK112_06165"
     CDS             1621..>2155
                     /locus_tag="PK112_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11667.1"
                     /translation="MPVIEKIRISDLPPYGKTFVGIFAGLAVCMLLWVALIYTVDRGI
                     VHSDRLPAYLNADRAGQGAPTTEDSARNERLEQEEIAEEAEEVAVDSEAVLAPIWDSD
                     LAGREVHADSASAAARFGKVDSTIEAEIARHGVGPGTGYDYNPDREDFDVDEFEQPNL
                     RRNAGLAHTHINGQTLIY"
BASE COUNT          408 a          650 c          730 g          367 t
ORIGIN      
        1 agagtgatcg accaactcct gatctccctg ctcgcctccg gtcactgcct gctcgtgggc
       61 gtgccggggt tggccaagac gctgttgatt tcgaccctgg cgcgggtgtt ggatctcaga
      121 ttcaatcgca ttcagttcac gcccgacctg atgccctcgg atatcaccgg aacagagatc
      181 atcgaggagg accaggcgac cgggcgacgg cggttccggt tcgtcaaggg accggtgttc
      241 gcgcacatca tcctggcgga tgagatcaac cggacgccgc caaagacgca ggcggcgctg
      301 ctgcaggcga tgcaggagca tgaggtgacg gcggcgggcg agacgtataa actcgacgaa
      361 ccgttcttcg tgctggccac ccagaaccct atcgagcagg agggaaccta ccccctgccg
      421 gaggcgcagc tcgaccggtt catgttcaac gtgttcgtcg attacccctc ggagcgggag
      481 gagcgggaga ttgtgcgatc gacgacggcg gtgctggact accagttgaa caagatcctg
      541 tcgccgcagg agattatcgg cttccagcag ctcgtgcggc gggtgccggt ctcggaccac
      601 ctggtcgagt acgcggtgaa gctggcgcgc aagacgcgcc cgcacgagcc ggacgccccc
      661 gacttcgtgc ggaactgggt gaactggggg gcgggaccgc gggcgtcgca gtatctcatc
      721 ctggccgcca agacgcgcgc gatcctcgac ggacgtccga cgccggggcc ggaggatgtg
      781 cgctttgccg cctacccggt gctgcggcac cggatcgtga cctcgttcaa cgccgaggcc
      841 gacggggtgg atacgatgga gattatcaag cgcctcctgg agacggtgaa agagcagtag
      901 caacgagtcg cggtgaaagc gagggccccg gctgcgtgtg gcagccgggg tctttttttt
      961 ggccggtcgg cgaacttgtt ttcgcctctg gacaaactca cgcaaacatc tacactcaag
     1021 ccatcagatt acgcagagtc cagcgcaggt aaccttgttg tcggcgagaa agcatgacca
     1081 cgagtgacga cagccgtgac cggacgcaga cccacatcca gttgacggtt gatctgatgg
     1141 tcgggcgcta ccgcttgacc ggcttctcgg gagcgggcgg gatgcgttaa gtccaccaag
     1201 cggaggacac cacgcccgga cgggcggtgg ccctgaagtt tctcccgctt cacttggcgg
     1261 cggatgccgg gggcagaaag cgctctcagg gggaggcgca ggcagtggcg agactcagtc
     1321 atccgaacat tgtcaccatc cacgaggtcg gggggtttca aggccggccg tcgggcgtcc
     1381 gcaagcaacg aggtcaggcg ggttcgcgtc ggcagcgacg gtctcgacac ggtcggcaca
     1441 ctgcctgaag gggcgtcaaa cgtcaaactc ctcccaggcg agaagtccgt cgtttactcg
     1501 gtcatgcact cccgctcgga catccggata gtcgagaatt ttggccccga ggtgcggcgc
     1561 taggccgccc tctccccttc ccgaaaaggg tttgcatccc tgtaccgatt gtatcatcgt
     1621 atgcccgtga tcgagaaaat tcggatatcc gacctgccgc cctacggaaa gaccttcgtc
     1681 ggcatcttcg ccgggctggc ggtctgcatg ctcttgtggg tggcgctcat ctacacggtc
     1741 gatcggggaa tcgtgcactc cgaccggctg ccggcctacc tgaacgccga ccgggccggg
     1801 cagggggcgc ccaccacgga agattcggca cggaacgagc ggctcgaaca ggaggagatc
     1861 gccgaagagg ccgaggaggt ggcggtggat tccgaagcgg tgctggcgcc gatctgggac
     1921 agcgatctgg cgggacggga agtacacgcc gacagcgctt ccgccgcggc gcggttcggg
     1981 aaagtcgatt cgacgatcga ggccgagatc gccagacacg gggtggggcc gggtaccggc
     2041 tatgactaca acccggaccg ggaggatttc gatgtcgatg agttcgagca gcccaacctg
     2101 cggcgcaatg ccgggctggc gcacacgcac atcaacggcc agacgctgat ttact
//
LOCUS       DAOOKS010000347         2154 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_64016_length_2154_cov_3.207718, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000347 DAOOKS010000000
VERSION     DAOOKS010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64016_length_2154_cov_3.207718"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2134)
                     /locus_tag="PK112_06170"
     CDS             complement(<1..2134)
                     /locus_tag="PK112_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11668.1"
                     /translation="MKTALLMPETVNQDNRATTMNAVLAHEFGHQLGLVDLYSTATFM
                     SQLGDFALMDNNGFGTTVDFGLPVGGVYGTMPLYPCAWSRAYLGYVQVYDFREGTRIP
                     LAAAQMVQEGIKVARIPITEKEYYLLENRIIEYDGLPTGQKQDTATGVLQGPVQVIIT
                     PQGDTVAGEFTREYDFLLPGSGMLIYHVDESVAGLDYDGDGVNNFDDNQLQWDPARRF
                     ISLVEGDGIVNFGGYYRAGWGAAADMYRDDRNRSFTPNTNPPTIDNTGNNTHIRITGI
                     TRKIDTLGASIVVRDSLMLFDVETENLVAGFPVRVGESTLPLSPIAADLNGDGTDEII
                     AAAGKRVVAFTTDGEGYFHKFFGTSDELFFDSAKARVHPGRPYPVPVFATAQSGQVLS
                     AGPVAGAFGNLNDTLHIAVGYRRESDSLGRNGNVIIYRATDFDQNGLAVSAAGFATNG
                     RGFPAALAFGDVLWVLTSEGHVYRRSSLMDSAIELGSFPMDEYHGICRAGSGLVLVGG
                     DETHTVWHAIDSTGASNALTVDGHYRFGPVLADVDRDGVPEAAGFSAEGDAILIAVDT
                     TVSPAALEVKAQKRTGLSVRANPAAADMDDDGYPDLVVGGVNQVAAFDRQLTLLADFP
                     KELDDRFPEAEVIAAPVVDDIQRGGRPEVIAPTEPGNIYAYGDRAATGFPLSAGEFCI
                     GSPLVVHDSLGGKLGYLGLDGWFYLWHTN"
BASE COUNT          367 a          793 c          625 g          369 t
ORIGIN      
        1 gattggtgtg ccacaggtag aaccagccgt cgaggccgag gtacccgagc ttcccgccga
       61 gcgagtcgtg caccaccagc ggcgacccga tacagaattc gccggccgaa agcgggaacc
      121 cggtcgccgc gcggtcgcca taggcataaa tattccccgg ctccgtcggc gcgatcacct
      181 ccggccgccc cccccgctga atgtcgtcga ccaccggggc cgcgatcacc tccgcctccg
      241 ggaaccggtc gtcaagttct ttcgggaaat ccgccagcag cgtgagctgc cggtcgaacg
      301 cggccacctg gttcacgccg ccgaccacca ggtcgggata gccgtcgtca tccatgtccg
      361 ccgccgccgg gttggccctc acgctcagcc cggtgcgctt ctgcgctttc acctccagcg
      421 ccgccggcga cacggtggtg tcgaccgcga tcagaatcgc atccccctcc gcggaaaagc
      481 ccgccgcctc cggcacgccg tcccggtcga catccgccag caccggcccg aaccggtagt
      541 ggccgtcgac cgtgagcgca ttgctggccc cggtcgagtc gatggcgtgc cagaccgtgt
      601 gcgtctcatc gccgccgacc agcacgagcc cgctccccgc ccggcagata ccgtggtact
      661 cgtccatcgg aaacgacccc agctcgattg ccgaatccat caaagaagac ctgcggtaga
      721 cgtgtccctc gctggtcagc acccacaaga cgtcgccaaa agctaacgcc gccgggaaac
      781 ctcgaccatt cgtcgcgaaa ccggcagcag atacagctag gccattctga tcgaagtccg
      841 tagcacgata gatgattaca ttgccgttcc tgcccaatga atccgactct cttcggtacc
      901 ctactgctat atggagcgtg tcattcagat tcccgaaagc gccggcaacc ggcccggccg
      961 aaaggacttg ccctgactgg gccgtcgcga acaccggcac cggatagggc cgccccggat
     1021 gcactctcgc cttggccgag tcaaaaaaca gctcgtccga ggtgccgaaa aacttgtgga
     1081 aatatccctc cccgtccgtc gtgaacgcca ccacccgctt gcccgccgcc gcgatgatct
     1141 cgtcggtccc gtccccgttg agatccgccg cgatcggcga caacggcagc gtcgactccc
     1201 cgacccgcac cgggaacccg gccacgagat tctccgtctc cacgtcgaac agcatgagcg
     1261 aatcacgcac gacgatcgat gccccgaggg tgtcgatctt gcgcgtgatc cccgtgatcc
     1321 ggatgtgggt gttgttgccc gtgttgtcga tcgtcggcgg gttcgtgttc ggcgtgaacg
     1381 accggttgcg gtcgtcccgg tacatgtcgg ccgccgcgcc ccaccccgcc cggtagtagc
     1441 cgccgaaatt gacgatcccg tccccctcca ccagcgaaat gaaccgccgc gccggatccc
     1501 actggagctg gttgtcgtcg aaattgttca ccccgtcccc gtcgtagtcg agtccggcga
     1561 cgctctcgtc gacgtggtag atgagcatgc ccgagcctgg caggaggaag tcgtactcgc
     1621 gggtgaactc ccccgccacc gtgtccccct ggggcgtaat gatcacctgg accggcccct
     1681 ggagcacgcc ggtcgccgtg tcctgcttct gccccgtcgg cagcccgtcg tactcgatga
     1741 tccggttctc cagcaggtag tattcctttt cggtgatggg gatgcgcgcc accttgatcc
     1801 cctcctgcac catctgcgcc gccgccagcg ggatcctcgt gccctcgcgg aagtcgtaga
     1861 cctggacata cccgaggtag gcccgcgacc aggcgcaggg gtagagcggc atcgtcccgt
     1921 agacgccgcc gaccggcagc ccgaaatcga ccgtcgtccc gaacccgttg ttgtccatga
     1981 gcgcgaaatc ccccagctgc gacatgaagg tcgccgtgct gtacaggtcg accagcccga
     2041 gctggtgccc gaactcgtgc gccaggaccg cgttcatcgt cgtcgcccgg ttgtcctggt
     2101 tgaccgtctc cggcatcagc agcgccgtct tcacccagtt cgccccctcg tcca
//
LOCUS       DAOOKS010000348         2139 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_64689_length_2139_cov_1.708733, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000348 DAOOKS010000000
VERSION     DAOOKS010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64689_length_2139_cov_1.708733"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="PK112_06175"
     CDS             complement(<1..752)
                     /locus_tag="PK112_06175"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002696122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HPC11669.1"
                     /translation="MTTKSEQKKNDELMDKLVSLCKRRGFIYPSSEIYGGLSSCWDYG
                     PLGAELKRNLKTVWWNAMTHRRDDVEGLDAAIFMHPQVWHTSGHVTEFHDPMVDCKKC
                     KARFRADKLESSRCPLKPSKSPLECGGELTEARQFNLMFKTHMGPMEDSASEVFLRPE
                     TAQGIYVNFLNVKNSSRQKISFGIAQIGKAFRNEITPGNFIFRTREFEQMEMQYFVAP
                     GEDEKWMEYWRAQRWQFYLDLGIREARLRWHQ"
     gene            complement(776..1567)
                     /gene="recO"
                     /locus_tag="PK112_06180"
     CDS             complement(776..1567)
                     /gene="recO"
                     /locus_tag="PK112_06180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HPC11670.1"
                     /translation="MAIKATEAILLRAHNWSESSRTVVFFTRDFGRLALTDRGGRRLT
                     GKRGRLVPFARLELTFYHSEKESAGYLSDVSLVQAWQFEREGTLGRLAFASAAIELLL
                     SLLGDRAPHPDLYTYTVSYLELVETAPKQGLGPLFVNYYIRLISRLGYHPSLTRCVAC
                     GATGERLADAAGGVQFSAERGGMVCRACESVGDCYIPFSSESHRLIRALEGASLGQAA
                     TLPISYRETVRLTEALTRFLSFQADVRAELKALTFLEKLRKDAEA"
     gene            1844..>2139
                     /locus_tag="PK112_06185"
     CDS             1844..>2139
                     /locus_tag="PK112_06185"
                     /inference="COORDINATES: protein motif:HMM:NF019247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 family protein"
                     /protein_id="HPC11671.1"
                     /translation="MQTEPHPEHIWLQRFVGDWTIEIQAVMGPGEPPTTHTGRETVRS
                     LGGLWIVAEGHGETPGGSFDSILTLGFDPQRRRFVGSFIASVMKLPTNRRRCGS"
BASE COUNT          375 a          756 c          623 g          385 t
ORIGIN      
        1 tgctggtgcc agcgcaggcg ggcctcccgg atccccaggt cgagatagaa ctgccagcgc
       61 tgcgcccgcc agtactccat ccacttctca tcttcgcccg gcgcgacgaa atactgcatc
      121 tccatctgct cgaactcgcg cgtgcggaaa atgaaatttc ccggggtgat ttcgttgcgg
      181 aacgccttcc cgatctgggc gatcccgaac gagattttct ggcgcgagga gttcttgacg
      241 ttgaggaaat tgacatagat cccctgcgcc gtttccggcc ggaggaacac ctcgctcgcg
      301 gaatcctcca tcggccccat gtgcgtcttg aacatgaggt tgaactggcg cgcctcggtc
      361 agttctccgc cgcactccag gggcgacttc gacggtttca ggggacagcg cgaggattca
      421 agcttgtcgg cccgaaaacg cgccttgcac ttcttgcagt ccaccatggg atcgtggaat
      481 tcggtcacgt gccccgaggt atgccacacc tgcgggtgca tgaagatcgc ggcatcgagc
      541 ccctcgacat cgtcccgccg gtgcgtcatc gcgttccacc acaccgtctt gagattccgc
      601 ttcagctcgg caccgagggg accgtagtcc cagcacgacg acagcccgcc gtagatctcg
      661 ctggaggggt agatgaagcc gcgccgcttg cacagcgaca ccagcttgtc catcaactcg
      721 tcattcttct tctgttcgct cttggtcgtc ataccgtctt cgtgctttcc tttcctcagg
      781 cttcggcgtc cttcctgagc ttctccaaaa aagtcagcgc tttcagctcc gcccgcacat
      841 ccgcctggaa gctcaggaac ctggtcagcg cttctgtcag ccggaccgtc tcacggtaac
      901 ttatcggaag cgtggccgcc tgtcccaacg acgctccctc cagcgcccgg atcagccggt
      961 ggctctccga agaaaaggga atatagcaat ctccgaccga ttcgcaagcc cggcagacca
     1021 ttcccccacg ctcggccgag aactgcaccc ccccggcggc gtcggccagc cgctcccccg
     1081 tcgccccgca cgccacgcac cgggtgagcg aggggtggta cccgagccgc gagatcagcc
     1141 ggatgtagta attcacgaag agcggcccca gcccttgttt cggcgccgtc tccaccaatt
     1201 cgaggtagga aaccgtgtag gtatagaggt ccgggtgcgg cgcccggtcc cccagcagac
     1261 tcagcagcag ttcgatcgcc gccgaggcga aagccagccg cccgagcgtc ccctcccgct
     1321 cgaactgcca cgcctgtacg aggctgacgt ccgacaggta ccccgccgat tccttctccg
     1381 agtggtagaa cgtcagctcc aggcgggcga acggaacgag ccgcccccgt ttgccggtca
     1441 gccgccgccc gccccggtcg gtcagagcca gccgcccgaa atcgcgggtg aaaaacacca
     1501 ccgtccgcga ggactccgac cagttgtgcg cgcgcaggag aatcgcttcc gtcgccttta
     1561 tcgccatggt ctcaccgcag ttcccgtttt gagtgcgggt cggcgccgcc cctatgccgg
     1621 cggcccggac cgcgccccgc aataacggca ttttgcggct gctgcgccac cgctaagcgc
     1681 tctgcccccc gccggctgcg ttcgaaaggc ttccgggccg cgaccggccc ggtccgggcg
     1741 agtgaacatg ggtcaaaaag gttcacaatg tgattgcgcc cggccgcccg agggcctatc
     1801 ttgccctcac cggacagtaa acctcttgaa atgggagatc gctatgcaga ccgaaccgca
     1861 cccggaacac atctggctgc agcgctttgt cggcgactgg accattgaga tccaggcggt
     1921 gatgggaccc ggcgaaccgc cgaccaccca caccgggcgc gaaaccgtcc gctccctcgg
     1981 cgggctgtgg attgtcgccg aaggccacgg ggagacgccc ggcggctcgt tcgactccat
     2041 cctcacgctc ggctttgacc cgcagcgccg gcggttcgtc ggcagcttca ttgcctccgt
     2101 aatgaagctg ccgacgaacc gccggcgctg cgggtcaaa
//
LOCUS       DAOOKS010000349         2134 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_64911_length_2134_cov_2.760943, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000349 DAOOKS010000000
VERSION     DAOOKS010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64911_length_2134_cov_2.760943"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..870)
                     /locus_tag="PK112_06190"
     CDS             complement(<1..870)
                     /locus_tag="PK112_06190"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HPC11672.1"
                     /translation="MPERQDAYIISACRSAIGVLHGGIGAFTTTQLGALAVKEAVRRA
                     GINPADIEEVIMGQVVQGGANQAPARQAAIHGGVPPEAAALTINKVCGSGLKAVMLAA
                     QSIRAGDQDVIVAGGMESMSNTPLALFGAKQGVKFGHQRLLDLMIIDGLWDSFNNFHM
                     GSAAELTRKKANITREMQDEFAYNSHKKALAAWNAGKFEAEIFPIEIPQRKGDPVVFK
                     KDEGPRPDISVEKLASLKPAFEKDGTVTAGNAPGLNDGSACTVIVSERYLKRHNLTPL
                     ARVVDYAVAGTPPA"
     gene            1097..1696
                     /locus_tag="PK112_06195"
     CDS             1097..1696
                     /locus_tag="PK112_06195"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HPC11673.1"
                     /translation="MNFSRPAARPLTARLPVLLLALALSAAPAGVSARIVYGSLQAGL
                     SIPAQGNRLNDRFDRGFGLGGGAVFVLGEEVNMIVQIEYHSLRNAGLPPLADRGSLRA
                     AMLTTMFRYMFGQDLDPWRPFLGLGFGTADLSLAKEGDTYTEWDGDDLHGVFHVGAGL
                     QVRVLDRFWIIAQARWMTISSPGGGDASTLYPISLGLEF"
     gene            complement(1794..>2134)
                     /locus_tag="PK112_06200"
     CDS             complement(1794..>2134)
                     /locus_tag="PK112_06200"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11674.1"
                     /translation="VRHKDKFYIDIDSVLLEVREATEENVAGAAGDHAVDPDKIFAPM
                     PGKIVKILVEVGQTVAEKQQMVIVEAMKMENPVLARASGTVKAINYRDGEQVGTEAPI
                     IELELAKKGE"
BASE COUNT          300 a          779 c          616 g          439 t
ORIGIN      
        1 cgccggcggc gttcccgcca ccgcgtagtc gaccacccgc gccagcggcg tgaggttgtg
       61 gcgcttgagg tagcgctccg agacgatcac cgtgcacgca ctgccgtcgt tgagccccgg
      121 cgcattcccc gctgtcactg tcccgtcctt ttcaaacgcc ggtttcagcg aggccagttt
      181 ctccacggag atgtcgggcc gcggcccctc gtccttcttg aaaacgaccg ggtcgccctt
      241 tctctgggga atctcgatgg ggaagatctc ggcctcgaac ttccctgcgt tccacgccgc
      301 gagcgccttc ttgtgcgaat tgtaggcgaa ctcgtcctgc atctcgcggg tgatgttggc
      361 cttcttgcgc gtcagctcgg ccgcgctgcc catatggaaa ttgttgaagc tgtcccacag
      421 cccgtcgata atcatgaggt cgagcagtct ctggtgtccg aatttcaccc cctgcttggc
      481 gccgaacagc gccagcggcg tgttggacat cgactccatg ccgcccgcga caatcacatc
      541 ctggtcgccc gcccggatcg actgggccgc cagcatcacg gctttcagtc cagacccgca
      601 caccttgttg atcgtgagcg cagcggcttc cggcggcacc ccgccgtgaa tggctgcctg
      661 ccgggcgggt gcctggttcg cccctccctg cacgacctgg cccatgatga cttcctcgat
      721 gtccgcgggg ttgatgcccg cgcgccggac tgcttcttta accgccagcg cgcccaactg
      781 ggtcgtggtg aaagcgccga ttccgccgtg cagcaccccg atcgcgctgc ggcaggcgga
      841 aatgatgtac gcgtcttgcc gttccggcat atgctgttac ctccattgca ggaatgatct
      901 cgcagtctga cgcctatata acgaaccccc ccggcgaaat caagtgaaca tattcacaaa
      961 tttcccccgc cggctcactc gaaccgcctc cctcccccgc ccggccgccc gtctctgccc
     1021 gtccggacat ttcgtttgtc ccgtgtcccc gtccgggcta ttttgttccg gcaatcgctt
     1081 ctcgaaagga gaccggatga acttctcccg ccctgcggcc cggccgctga cggctcgcct
     1141 gccggtcctg ctgctggctc tggccctgag cgccgccccg gccggcgtct ccgcgcgaat
     1201 cgtctacggc tcgctccagg ccggtctgtc gatacccgcc cagggcaacc gtctgaacga
     1261 ccgctttgac cgcggattcg gtctcggcgg cggcgccgtg ttcgtgctcg gtgaagaggt
     1321 caacatgatc gtccagatcg aataccacag cctccgcaac gccggccttc ccccgctggc
     1381 cgaccgcggc tcgctgcgcg ccgccatgct caccacgatg ttccggtaca tgttcgggca
     1441 ggatctggac ccctggcggc cgttcctcgg ccttggtttc ggcaccgccg acctatcgct
     1501 ggccaaagaa ggggacacct atacggagtg ggacggcgac gatctccacg gcgtgtttca
     1561 cgtcggcgcc ggcctccagg tgcgcgtgct cgatcgtttt tggattatcg cccaggcgcg
     1621 ctggatgacc atttcctcgc ctgggggcgg cgacgcgagc actctctatc ctatttcgct
     1681 cgggctcgag ttctagggcc ggacccggcc cgcccgcaga gagtcggccg gcgcgggcgc
     1741 cgctcagccc gacagatgtc ggtcgggccg ctgcgcccgc ggcgttgatt ccctcactcg
     1801 cccttcttcg ccaattccag ctcgataatc ggggcctcag tccccacctg ctccccgtcg
     1861 cggtagttga tcgccttgac tgtcccgctc gcccgcgcga gcaccgggtt ttccattttc
     1921 atcgcctcga cgatcaccat ctgctgcttc tcggcgaccg tctgccccac ctcgaccagg
     1981 atcttgacga tcttccccgg catgggcgcg aagattttgt ccggatcgac ggcgtggtcg
     2041 cccgccgccc cggcgacatt ttcctcggtc gcctcccgca cctccaggag gaccgagtcg
     2101 atatcgatat agaacttgtc cttgtgccgc accg
//
LOCUS       DAOOKS010000350         2132 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_64998_length_2132_cov_3.674049, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000350 DAOOKS010000000
VERSION     DAOOKS010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64998_length_2132_cov_3.674049"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            424..699
                     /locus_tag="PK112_06205"
     CDS             424..699
                     /locus_tag="PK112_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11675.1"
                     /translation="MVSGTKKFYLTTMTDNTSDEFEIRNLRLTAMFFPTAYGSVEATG
                     SSADTPEHSELYDARGAVEAERAAELEAQRRLIEAKVQGSARNKSDK"
     gene            1214..1465
                     /locus_tag="PK112_06210"
     CDS             1214..1465
                     /locus_tag="PK112_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HPC11676.1"
                     /translation="MLNILWYIIVGFLAGLVARAILPGADHMGFFATTAVGIVGGILG
                     GLIGDRFSKTPSAAKFRPAGFIMSVIGAVILLIVLRLLR"
     gene            complement(1670..>2132)
                     /locus_tag="PK112_06215"
     CDS             complement(1670..>2132)
                     /locus_tag="PK112_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11677.1"
                     /translation="VFDFINALAFAHGHLFVGTDEALYVYDGGRWETVTTDDGLPSNR
                     IRSIDASGWVVYLGTPAGVASWFNYDVAPVARLEDRAATLVRTFGRKLIVATEREGVL
                     LKAGPVVSTLIRAETIPVLAEAAPQFSEPLPAGALVDSADAVGAQPVAAAK"
BASE COUNT          379 a          741 c          629 g          383 t
ORIGIN      
        1 ggtctcctgc cgcttgatgg cgggcagcat cccggaggct gatctgaaga gtcctttgat
       61 catagtattt ctccggggat tcctaaagca ggaggcgtgc cacaaccggc tctcgcccat
      121 aaggtcttgc tcagtaacaa cttggagagg cccccgaaac cgcgggcgga ctgccttcgg
      181 gaaagaaatt cagatccggt acggacgatt tttccctctc cctgcgggaa taccggatcc
      241 gggtggccct gggcgccgat ccagcacggt gacacgtcaa cgcgcaggtc ggacgcagcg
      301 cagcctcggg ccgcatttcg gcgacgcgct cccaatcacg ccggtcgatg cgtggtcgag
      361 gccagagcga actacgcatc cagtgaccat tacgttccgt tctccctcac ccgagtgcag
      421 ccggtggtga gcgggacaaa gaagttctac ctgaccacga tgaccgacaa tacttccgac
      481 gagttcgaga ttcggaatct ccgcctgacg gcgatgttct tcccaaccgc atacggtagt
      541 gtggaggcca ccgggtcgtc cgctgacact ccggagcact cggagctata tgacgcccgg
      601 ggcgcggttg aagcggagcg ggcggccgag ctggaggcac aacgcaggtt gatcgaggcg
      661 aaagtccagg ggtcggcccg gaacaaatcg gacaagtaga atgaagtaga cgctacttga
      721 tgcggcagtc atccgcggcc caggacgacc cgacttctgg tggacggcgg tcgatttgac
      781 cggcgcttac cgatacgcgg ctcttggcgg ccgcccatgc atacgccttt ccctggctcc
      841 aacgcgcgta cgggcggggt ctgcctctgc cccgacgcaa gggaaccgga catgcgactc
      901 tcgtgagaga gaaccggccc acagattatc cgagtgacga tgacattcct taggcgccgc
      961 ttccgtcttg ggctcgcccc cctgccctat gcttccccat gcctcctttg gcaggttcca
     1021 gctcatcaat cccccccgca ccacctggtc tgctgccgac accgtcgaat tccgcttgcc
     1081 ccccattttg acaactgcgt aattgaccgc gaaactaggc gcctgcgctg acacggaaat
     1141 acctgttctc ccgcgtcagc ctcccgggcg cctccggttc gattgggatc ctgcagtctg
     1201 aaaggagctc tccatgctca acattctctg gtacattatc gtcggcttcc tggccggcct
     1261 cgtcgcgcgg gcgatcctgc ccggcgccga ccacatgggc ttcttcgcca ctacagccgt
     1321 gggtattgtc ggaggcatcc tcggcggtct catcggcgac cgcttcagca aaaccccgag
     1381 tgcggccaaa ttccgtcccg ccggctttat catgtccgtc atcggcgccg tcatcttgct
     1441 cattgtcctc cggctcctcc gctgacaccc gacagagcct cgccgcgcgg gagtccctcg
     1501 gctcccgcgg cttcccggag gcgagggtat cccggcgcgg tctccgtctc ctgcgtcaaa
     1561 ctaacaaagc aacggtcccc caaaacgccg caggcccccc gggaggagcg cggcgccgga
     1621 gagcctgcgg gtgcgataga atcgcgctat cggttatgat cgtcgtgcct catttcgccg
     1681 ccgccaccgg ctgggcgccc actgcgtccg ccgaatcgac cagcgccccg gccgggagcg
     1741 gctcggagaa ctgcggcgcc gcctcggcca acaccgggat cgtctccgcc cggatcaacg
     1801 tcgacaccac cgggcccgct ttcagaagca ctccctcccg ctcggtcgcc acgatcagct
     1861 tgcggccgaa tgtccgcaca agggtggcgg cgcggtcctc gagcctcgcc accggcgcca
     1921 catcgtagtt gaaccacgac gctactcccg ccggtgtccc gaggtaaacg acccaccccg
     1981 aggcgtcgat cgacctgatc cgattcgacg gcagcccgtc gtcagtcgtc accgtctccc
     2041 accgcccgcc atcgtaaaca tagagcgcct cgtcggtgcc gacaaagagg tggccgtggg
     2101 cgaaggcgag ggcgttgatg aagtcgaaca cc
//
LOCUS       DAOOKS010000351         2127 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_65229_length_2127_cov_3.089768, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000351 DAOOKS010000000
VERSION     DAOOKS010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65229_length_2127_cov_3.089768"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="PK112_06220"
     CDS             <1..427
                     /locus_tag="PK112_06220"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HPC11678.1"
                     /translation="SEEARLQTAYRNYNWTVPPYRPSDSLRLRLNSKGDAWTTVFADG
                     DTSVVRNLRPQEDVLISARHRLLVSIAVPSKVTVELNGRPVDLVSPETGRISLVEITQ
                     ANLDSIFTAARPDTADTLAPADSGGAPAAPAPPAGGATP"
     gene            429..1046
                     /locus_tag="PK112_06225"
     CDS             429..1046
                     /locus_tag="PK112_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11679.1"
                     /translation="MLAGIKRFTARSQLSRLRKQMTPGTFQYPEALRTVRKILVCLPA
                     ELRELTTVKQFLPEIRDLFKTADITLLTAPGLQIADIFPRKGFNILTPSADQLNWSGL
                     ARKSYLKFLQDYRFDVVIDFGLEATDFTSSVLLSLPQAVRIGRGNHLGCPYYNLQIKT
                     RYLRDERNIYRSLLDTLRLLIDGRPKDEPAGPPPDDRRHGVSQET"
     gene            1024..>2127
                     /locus_tag="PK112_06230"
     CDS             1024..>2127
                     /locus_tag="PK112_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011361465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPC11680.1"
                     /translation="MVYLKKLDILGFKSFANKTTVHFSHGITAVVGPNGCGKTNILDS
                     LRWVLGEQKTSLLRGAKMEEVIFNGTAEVKPMGMAEVSLTVVNDRGILPTEYSEVQIT
                     RRLFRSGESEYLLNRVPCRLRDIQELFYDTGVGAHSYSVIQQDMIDAVISDKAEERRF
                     LFEEAAGITKYKQRKRIALRKLEATENDLLRLKDIYAEVQTQVRSLKRQYRKAERYQQ
                     ELDQVKAWELYLGARRVRAIEAEKRELRAVLDELADGMTRGQTTVDRLSADIESERRR
                     QTELEHHLVEIGAAVYEVTERAHALEKEISVLTEKRHSARALVERNERELAAMRERAA
                     VLDRQQSEATALGERQRAEFEDTARQVADAENAQ"
BASE COUNT          400 a          760 c          636 g          331 t
ORIGIN      
        1 aagcgaggag gcgcgtctgc agaccgcgta ccgcaactac aattggaccg ttcctcccta
       61 ccggccctcc gactcgctgc gcctgcggct gaactcgaag ggggatgcct ggacgacggt
      121 ctttgccgac ggcgacacgt ccgtcgtccg caacctgagg ccccaggagg acgtgctcat
      181 atcggcgcgc caccggctgc tggtgtcgat cgccgtcccc tccaaagtga ccgtcgagct
      241 gaacggccgc ccggttgacc tggtctcgcc ggagaccggg cgcatctcgc tggtggagat
      301 tacacaggcc aatctggatt ccattttcac cgctgcgcgc cccgacaccg ccgatactct
      361 cgcgcccgcc gattccggcg gcgctccggc ggcgccggcc ccgcccgcgg gaggagcgac
      421 gccctgacat gttggccggc ataaagagat tcacagcgcg ctcccagttg agccgcctgc
      481 gcaagcagat gacgccgggg acattccagt accccgaggc tctccgcacg gtgcgcaaaa
      541 tcctcgtctg tctccccgcc gagctccgcg agctgacgac cgtgaagcag ttccttccgg
      601 aaatccgtga cctgttcaag accgccgaca tcaccctgct caccgccccc ggccttcaga
      661 tcgccgacat cttcccccgc aaagggttca acatcctcac cccgtcggcc gaccagctca
      721 actggagcgg gctggcccgg aagagctatc tgaagtttct ccaggactac cgcttcgacg
      781 tcgtgatcga cttcggtctg gaggcgaccg atttcacctc gagcgtgctc ctctcgctgc
      841 cccaggcggt gcgcatcggg cgcggcaacc acctcggctg cccctactac aacctgcaaa
      901 tcaagacgcg gtacctgcgg gatgagcgca acatctaccg gtctcttctc gacacgctgc
      961 ggctgttgat cgacggtcgt ccgaaagacg aaccggccgg accgcccccc gatgatcgga
     1021 ggcatggtgt atctcaagaa acttgatatc ctcggcttca aatcgttcgc caacaaaact
     1081 acagtccact tttcccacgg catcacggcc gtcgtcgggc ccaacggctg cggcaagacc
     1141 aacattctcg actcgctgcg ctgggtcctt ggcgaacaga aaacctccct cctccgaggc
     1201 gccaagatgg aggaggtcat attcaacggc acggccgagg tcaagccgat gggcatggcc
     1261 gaggtctcgc tcacggttgt caacgaccgc ggcattctcc cgaccgagta ttccgaggtt
     1321 cagatcacgc gccgcctctt ccgcagcggg gaatcggaat acctcctcaa ccgcgttccc
     1381 tgccgcctcc gggacatcca ggagctcttc tacgacaccg gcgtgggggc ccactcctac
     1441 tcagtcatcc agcaggacat gatcgatgcc gtcatttccg acaaagccga ggagcgccgt
     1501 ttcctcttcg aggaggcggc cggcatcacc aagtacaaac agcgcaagcg catagcgctg
     1561 cggaagctcg aggccaccga gaacgacctc ctgcgcctca aggacatcta cgccgaggtc
     1621 cagacccagg ttcgctcgct caagcgccag taccgcaagg ccgagcgcta ccagcaggag
     1681 ctcgaccagg tgaaggcctg ggagttgtat ctcggcgccc gccgcgtccg ggcgatcgag
     1741 gcggagaagc gcgagctgcg cgccgtcctc gatgaactcg ccgacggcat gacccggggc
     1801 cagacgacgg tcgaccgcct ctcggccgac atcgaatccg agcgccggcg gcagaccgag
     1861 ctggagcacc acctggtgga gatcggcgcc gcggtctacg aggtgaccga acgcgcccac
     1921 gctctggaaa aggaaatctc cgttctgacc gagaagcgcc acagcgcgcg cgccctcgtc
     1981 gagcgcaacg agcgcgagct ggcggccatg cgcgagcggg ccgcggttct cgaccggcag
     2041 cagtccgagg ccaccgccct gggcgagcgg cagcgcgccg agttcgagga caccgcccgg
     2101 caggtggccg acgccgagaa cgcccag
//
LOCUS       DAOOKS010000352         2126 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_65269_length_2126_cov_3.014969, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000352 DAOOKS010000000
VERSION     DAOOKS010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65269_length_2126_cov_3.014969"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..1192
                     /locus_tag="PK112_06235"
     CDS             65..1192
                     /locus_tag="PK112_06235"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPC11681.1"
                     /translation="MATQREFWPFLGGVCLLAFGTAVFKPGVQGTLVRTLNNKNSSIG
                     WGTFYMLVNIGGFLGPPLAHFFYGISWPAVFFGCAGIISLNYIMLFTYKEVHAGGEQK
                     GGPLHILAITARNFLNGKLVVFILIMSGFWLMFMQLFDMLPNFIVDWVNSASLVKTLG
                     LPDWMLQLNSDRAPQLSQEWMINANSGLIILLVVPLSWMVSRLRRVHSITIGVIISSV
                     GLVLAGFTTAGVFCILGIMVFSVGEMLASPKMNEYLGVIAPEGQKGLYMGYANVPLAV
                     GWGYGSLYGGQVYGTMGEKAKLAADYLAAKYQVLDVARTEAFAKLQEVSGMNAQEATD
                     ALWHAYHPYKLWYQFAAVGLLSAAAMVWYSWWVKRDEKANA"
     gene            1345..>2126
                     /locus_tag="PK112_06240"
     CDS             1345..>2126
                     /locus_tag="PK112_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11682.1"
                     /translation="MAGTLSGRRGSRGALALRRLSFIAALILCAAAPAGGKDLYHVTL
                     TGPTDAEALRGLGLDAVARVADGYLVLIDPPQAAALETSALRSRLVRANIARSDLARD
                     LRTDPGDRARYPVVFESGGLRLVDVRGGIVPDENGWLGLAPLPSTSLPIVCRTARPIG
                     RARPERALDLDSLISLINEDSCRSYVERLQAFGTRKVGTSQNAASRDWLVDKFRSFGY
                     DSVITDSFAAYENWGDGPAVAAFNVIAVKPGTLYPDFHILIGA"
BASE COUNT          340 a          645 c          730 g          411 t
ORIGIN      
        1 gaccgctacg gctacaagaa gacgatggcc ttcgcgttca cggtcaagat tatcgtacct
       61 cacgatggcc acgcagcggg agttctggcc cttcctcggc ggcgtgtgcc tgctggcgtt
      121 cgggacggcg gtgttcaagc cgggggtgca ggggacgctg gtgcggacgc tgaacaacaa
      181 gaattcgtcg atcgggtggg ggacgttcta catgctggtg aatatcgggg ggttcctcgg
      241 cccgccgctg gcgcacttct tctacggtat ctcctggccg gcggtgtttt tcggctgcgc
      301 cgggatcatc tcgctcaact acatcatgct cttcacctac aaggaagtgc atgcgggggg
      361 ggagcagaag ggggggccgc tgcacatcct ggcgatcacc gcccgcaatt tcctcaacgg
      421 caaactggtc gtgttcatcc tcatcatgtc ggggttctgg ctcatgttca tgcagctgtt
      481 cgacatgctg cccaatttca ttgtcgactg ggtcaattcc gcgtcgctgg tgaaaacgct
      541 cgggctgccc gactggatgc tgcagctcaa ttccgaccgg gcgccccagt tgtcgcagga
      601 gtggatgatc aacgccaatt cggggctgat catcctgctc gtcgtgccgc tgtcgtggat
      661 ggtctcgcgg ctccggcggg tgcactcgat caccatcggc gtcatcattt cctcggtcgg
      721 cctcgtgctg gccggattca cgaccgccgg cgtgttctgc attctcggca tcatggtctt
      781 ctcggtgggc gagatgctcg cttcgccgaa aatgaacgag tacctcggcg tgatcgcgcc
      841 cgaggggcag aaggggctct acatggggta cgccaacgtg ccgctcgcgg tcggctgggg
      901 gtacggctcg ctctacggcg gacaggtgta cggcaccatg ggtgagaaag ccaagctggc
      961 cgccgactac ctcgccgcca agtaccaggt gctcgatgtc gcccgtaccg aggcgttcgc
     1021 gaaactgcag gaggtttcag gaatgaacgc ccaggaggcg accgatgccc tgtggcacgc
     1081 ctaccacccc tacaagctct ggtaccagtt cgccgcggtc ggcctgttgt cggcggcggc
     1141 gatggtgtgg tacagttggt gggtgaaaag ggacgagaag gccaacgcgt aggggagaac
     1201 agggcaccgc cggggagtgg atcctccgcc cgcccggaca gggcttgacg aaacagggag
     1261 tgcgcagtat actataggta gtcatcgccg tagaagttca accgaatgca agccgagccg
     1321 ggccgcccgg cgagaggtgc gagtatggcc ggcacgctgt caggacggcg cggcagcagg
     1381 ggtgccctgg ccctccgccg cctgtcgttt atcgcggcgc tcattctctg cgcggccgcc
     1441 cctgcggggg ggaaagatct ctatcacgtt accttgaccg gtccgacgga cgcggaagcg
     1501 ctccggggtc tgggcctcga tgcggtggcc cgggtcgccg acggctacct ggtgctcatc
     1561 gacccgccgc aggcggccgc tctggagacg agcgcgctcc ggtcgcggct ggttcgggcc
     1621 aacatcgccc ggagcgatct ggcgcgcgac ttgcgaacgg atccggggga ccgcgcccgc
     1681 tatccggtag tcttcgagag cggggggttg cgtctcgtcg acgtgcgcgg ggggattgtg
     1741 ccggacgaaa acggctggct gggtctggcg ccgctgccat cgaccagtct gccgattgtc
     1801 tgccgcacgg cccggccgat cggccgcgcg cggccggagc gcgcgctcga tctcgactcg
     1861 ctgatcagtc tgatcaatga ggattcctgc cgttcctatg tggagcggct gcaggctttc
     1921 gggacgcgca aggtgggcac gagccagaat gccgcctcgc gcgactggct tgtcgacaag
     1981 ttccggtcgt tcggttatga ctcggtgatc acagacagct tcgccgccta cgagaactgg
     2041 ggagacggtc cggcggtggc cgcattcaat gttatcgccg tcaagccggg gacgctgtat
     2101 cccgattttc acattctgat cggcgc
//
LOCUS       DAOOKS010000353         2123 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_65405_length_2123_cov_2.778530, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000353 DAOOKS010000000
VERSION     DAOOKS010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65405_length_2123_cov_2.778530"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..1751
                     /locus_tag="PK112_06245"
     CDS             150..1751
                     /locus_tag="PK112_06245"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HPC11683.1"
                     /translation="MNVAAVLYALGKLMQVMGGFLLAPLAIALWDGRVQGLPRLFAAA
                     DASGLLVAAVFGLAVGTLLAALFRRGRGRQGLREGYAIVTIGWLTLTFVAALPLFLYF
                     WRVGMPDGSVSGLRAFTDAYFEIMSGFTTTGATILSNIEAVPRSLLFLRSLTHWLGGM
                     GIITLAIVIFPAMGVSAYSMYRGEVPGPSHDKLRPRLAQTAQVLWGVYVLLTALQTVL
                     LIFGGMTWFDAVNHSFATMATGGFSTRNASVAAYNSPYIEWVIIVFMYLAGVNFLLHF
                     KALRGDLRSLTHNSEFIFYSAVIALAALAATIVLYLQGLEPMAEAARSFRAHPPTETA
                     FLERYLTEQSRLEGLADCFRAALFQVVSVTTTTGFSTADFDMWPDFVRFLLMVLMFFG
                     SMAGSTGGGIKMIRVMVVAKAAANVVRKLTQPRLVAPVKVGGQPLDNDKIISIAALFV
                     FFTGLFVLTSMLLMLFIPDMITAASASIATLGNVGPGLAGVGAVENYGWIPAPAKWLL
                     VLSMLLGRLEIFTVLIVLRPAIWRK"
     gene            1860..>2123
                     /locus_tag="PK112_06250"
     CDS             1860..>2123
                     /locus_tag="PK112_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11684.1"
                     /translation="MRSFFPLIFATAVILVFGSVEILLLRLFNPSWWRVRWIRRTGLL
                     LPLVGTLCAGLWGLGEYYAASWLAAPGAAATALAFVLLVALMLS"
BASE COUNT          279 a          815 c          610 g          419 t
ORIGIN      
        1 catcatccgg ggcagttcca tgatccttcc cggcggcgac accgtcatcg aggcgggcga
       61 ccatgtcatc atgatctcca gccacaaaaa cctgaaaatg ctcgaccggc tcttctcggg
      121 ccggtgaccc tcatgccgga gccgtcgcca tgaatgtcgc cgccgtcctc tacgcactgg
      181 gcaagctgat gcaggtgatg ggcggcttcc tgctggcccc tctggccatc gcgctgtggg
      241 acggccgcgt acaggggctg ccccggctgt tcgcggccgc cgatgcctcc ggcctcctgg
      301 tcgcggccgt cttcggcctc gccgtcggca ccctcctggc cgcgctcttc cggaggggcc
      361 gcgggcgcca gggcctccgc gagggatacg ccatcgtcac catcggctgg ctcaccctca
      421 cctttgtcgc cgccctcccc ctcttcctct atttctggcg cgtgggcatg cccgacggct
      481 cggtgagcgg gctgcgggcg ttcaccgacg cctatttcga gatcatgtcc ggcttcacca
      541 ccaccggcgc caccatcctc agcaacatcg aggccgtccc ccgctccctc ctcttcctcc
      601 gctcgctcac gcactggctg ggcggcatgg ggatcatcac cctggcgatc gtcatcttcc
      661 ccgccatggg ggtcagcgcg tacagcatgt accgcgggga ggtccccggc ccctcgcacg
      721 acaagctccg cccccgtctc gcccagaccg cccaggtgct ctggggagtc tacgtgctgc
      781 tcaccgccct gcagacggtg ctgctgatct tcggggggat gacctggttc gacgccgtca
      841 accactcctt cgccaccatg gccacgggcg gcttctccac ccgcaacgcc tcggtcgccg
      901 cctacaattc cccatacatc gaatgggtca tcatcgtgtt catgtacctc gccggcgtca
      961 actttctcct gcacttcaag gccctccggg gcgacctccg gtcgctgacg cacaacagcg
     1021 agttcatctt ctattccgcc gtcatcgccc tcgcagccct ggcggccacc attgtgctct
     1081 accttcaggg actggagccg atggcggagg cggcgcgcag cttccgcgcc catccgccga
     1141 ccgagaccgc tttcctcgag cgctatctca cggagcagtc cagactcgag ggcctcgccg
     1201 actgcttccg cgccgccctg ttccaggtcg tctcggtcac caccaccacc gggttctcga
     1261 ccgccgattt cgacatgtgg ccggatttcg tgcgcttcct cctcatggtg ctcatgtttt
     1321 tcgggagcat ggccggttcc accggcggcg gcatcaagat gatccgcgtc atggtcgtcg
     1381 ccaaggcggc cgccaacgtc gtgcgcaaac tcacccagcc gcgcctggtc gcccccgtca
     1441 aagtgggcgg ccagccgctg gacaacgaca agatcatcag catcgccgcc ctgttcgtct
     1501 tcttcaccgg gctcttcgtc ctgaccagca tgctcctgat gctcttcatc cccgacatga
     1561 tcaccgcggc ctccgcctcg atcgccaccc tcggcaacgt cggccccggc ctggccggcg
     1621 tcggcgcggt cgaaaactac ggctggattc ccgccccggc caagtggctc ctggtgctct
     1681 cgatgctgct gggacggctc gaaatcttca ccgtcctcat cgtcctgcgc ccggctatct
     1741 ggcgcaaatg agcggaggga acctccggcc gcgcgcatcg ttatgatcga ggcaagctgt
     1801 gctgtcggtg atcccggcgc ggcgcccgcc gccgggaacc gggcaactca aaggatacta
     1861 tgcgttcgtt ctttccgctc atcttcgcca ccgccgtgat cctcgtcttc ggctccgtcg
     1921 agatcctcct cctgcgcctc ttcaaccctt cctggtggcg cgtgcggtgg atccggcgga
     1981 ccgggcttct gctgccgctt gtcggcacac tctgcgccgg gctgtggggg ctgggcgagt
     2041 actacgccgc ctcctggctg gccgcgccgg gcgccgccgc caccgccctc gctttcgtcc
     2101 tgctggtcgc cctcatgctc tcg
//
LOCUS       DAOOKS010000354         2120 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_65558_length_2120_cov_1.752058, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000354 DAOOKS010000000
VERSION     DAOOKS010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65558_length_2120_cov_1.752058"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="PK112_06255"
     CDS             <1..662
                     /locus_tag="PK112_06255"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164424.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 2 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine dehydrogenase"
                     /protein_id="HPC11685.1"
                     /translation="EAKGLFIVSSDRLALGTLEAPGKLGADIVVGDGQTAGNPMNFGG
                     PAFGFIAVNKPLMRKMPGRICGQTTDSQGRRTFVLTLQAREQHIRREKATSNICSNQN
                     LCIVAASVYLSLMGPQGLRDIGGQILAKSAYAVEQLEKTGKFRRAFPGVPSFRETALL
                     CEEPVDKLNARLLQQGILGGYSLEAEYPRLKNGWLLAVTESRTKEEIDRLVAVAGGDA
                     R"
     gene            659..>2120
                     /gene="gcvPB"
                     /locus_tag="PK112_06260"
     CDS             659..>2120
                     /gene="gcvPB"
                     /locus_tag="PK112_06260"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HPC11686.1"
                     /translation="MKRTMDTLFETSRPGRVGYALPELDVPAVDCGKELKGFCRAAAP
                     RLPELAEVDVVRHFTGLSKRNYSVDEGFYPLGSCTMKYNPKVNEVAARMPGFSRIHPL
                     QPEGTVQGALKLMYDLSAMLAEITGMDAVTLQPAAGAHGEQTGLMMIKAYHESRGDHK
                     RTKVIVPDSAHGTNPASAAVTGFDTVEVASDAEGMVDLDALKALMDDSVAALMLTNPN
                     TLGVFERNILALTETIHAAGGLVYYDGANANAIMGRARPGDMGFDVLHLNLHKTFSTP
                     HGGGGPGSGPVGCKKALAKYLPGPIVVKTQEGYALSESSEKSIGRVKTFYGNFLVALR
                     ALAYIEVLGKEGIKQAAQLAVLNANYMMARLKDKFPTHSSKICMHEFVMSCEEIKKEI
                     GVSALDFAKAMIDRGMHPPTIYFPLIVHEALMFEPTETESKATLDWACDTVLELLEEA
                     YRDPEAFKKHPCTMPIGRPDEVAAARKPIIIYERDSD"
BASE COUNT          434 a          699 c          653 g          334 t
ORIGIN      
        1 acgaggcgaa ggggctcttc atcgtctcct ccgaccggct cgcgctggga accctcgaag
       61 cgcccggaaa gctcggtgcc gacatcgtcg tcggcgacgg ccagacggcg ggcaatccga
      121 tgaacttcgg cggccccgcc ttcggcttca tcgccgtgaa caagcccctg atgcggaaga
      181 tgccgggacg catctgcggc cagactaccg acagccaagg gcgccgcacc ttcgtcctca
      241 cgctccaggc gcgcgagcag cacatccgcc gcgagaaggc cacgtcgaac atctgctcga
      301 accagaacct ctgcatcgtc gcggcctcgg tctacctcag cctcatgggg ccgcagggac
      361 tccgcgacat cggcggccag attctcgcca agtcagccta cgcggtggag cagctcgaaa
      421 agacgggcaa attccggagg gcgttccccg gcgttccgtc tttccgcgaa accgcgctcc
      481 tctgcgagga accggtggac aagctgaacg cccgccttct gcagcagggc attctcggcg
      541 ggtactccct ggaagcggaa tatccgcgct tgaagaacgg ctggctcctc gcggtaacgg
      601 agagccgcac gaaagaagag atcgaccgcc tcgtcgcggt tgcgggaggt gacgcccgat
      661 gaaacgcacg atggacacgc tttttgaaac cagccgcccc ggacgggtcg gctacgcgct
      721 gcccgaactc gacgttcccg ccgtggactg cggcaaggag ctgaagggct tctgccgcgc
      781 cgcggccccc agactgccag aactggccga ggtggacgtg gtgcgccact tcacaggcct
      841 ctcgaagagg aactactcgg tcgacgaggg cttctaccct ctcggctcgt gcaccatgaa
      901 gtacaacccg aaggtgaacg aggtcgccgc ccggatgccg ggcttctcgc gcatccaccc
      961 ccttcagccc gagggaacgg tccagggcgc gctgaagctc atgtacgacc tgagcgcgat
     1021 gctcgcggag atcacgggga tggacgcggt cacgctccag cccgccgccg gagcccacgg
     1081 cgaacagacc ggcctgatga tgatcaaggc gtaccacgag agccggggag atcacaagcg
     1141 gacgaaggtc atcgtccccg actccgcgca cggcaccaac ccggcgtcgg cggcggtcac
     1201 cggcttcgat acggtagaag tcgcctccga cgccgagggg atggtcgacc tcgacgcgct
     1261 caaggcgctg atggacgact ccgtcgccgc gctcatgctc accaacccga acacgctcgg
     1321 cgtcttcgag cggaacatcc tcgccctgac ggagaccatc cacgccgcgg gagggctcgt
     1381 ctactacgac ggggccaacg cgaacgccat catgggcagg gcgcgccccg gcgacatggg
     1441 gttcgacgtg ctccatctga acctccacaa gaccttcagc acgccgcacg gcggaggcgg
     1501 cccgggcagc ggtccggtcg gctgcaagaa ggctctggcc aaatatctgc cggggcccat
     1561 cgtggtcaaa acgcaagagg gctacgcgct ctcggagtct tcggagaaat ccatcggaag
     1621 ggtcaagacc ttctacggaa acttcctggt ggcgctccgt gcactggcct acatcgaagt
     1681 tctgggtaaa gaagggatca aacaggcggc tcaactggcg gtgctcaatg cgaactacat
     1741 gatggctcgc ctgaaggata aattcccaac tcattcctcg aagatctgca tgcacgaatt
     1801 cgtgatgagc tgcgaagaaa tcaagaagga gatcggcgtc agcgcgctgg attttgccaa
     1861 ggcgatgatc gatcgcggga tgcacccgcc cacgatttac ttcccgttaa tcgtccacga
     1921 agctctgatg ttcgagccga ccgagaccga atccaaagcc actctggact gggcttgcga
     1981 cacggtgctc gagctcctcg aggaagctta ccgcgaccct gaggctttca aaaagcatcc
     2041 ctgcacgatg cctattggcc gacctgacga ggtggccgct gcgcgtaaac cgatcatcat
     2101 ctacgagaga gattccgatt
//
LOCUS       DAOOKS010000355         2105 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_66214_length_2105_cov_2.968293, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000355 DAOOKS010000000
VERSION     DAOOKS010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66214_length_2105_cov_2.968293"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="PK112_06265"
     CDS             complement(<1..505)
                     /locus_tag="PK112_06265"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPC11687.1"
                     /translation="MIRAAAYSEALPGGGIVCTLCPVRCRFREGRRGVCGSRFMRDGE
                     LLVDNYGEAVTLALDPIEKKPLYHFHPGTDILSTGPNSCNLGCRHCQNWAISQERAET
                     KFVSPESLAAAARRYGAFGVAFTYTEPLMWFEYIRDVAPILRQAGLAVVLVTNGYIEA
                     APLEELIP"
     gene            complement(502..>2105)
                     /locus_tag="PK112_06270"
     CDS             complement(502..>2105)
                     /locus_tag="PK112_06270"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HPC11688.1"
                     /translation="SSDMEKILVIRFGSLGDVILTSAAVLNLKISFPGSRLTFFTKER
                     FGGLAAAFDGVDEVVCLPEGGDGRELVRLALELDRRGFDAVADLHGNFRSWLTRKMVT
                     APHATVYPKRRLARRRLLRGRNRLAPGPHTIDLYNQAVRAIGGKTPARRPLLPPPVPD
                     ADLPPSVREFLGRHQVCVAAAPGAAHRPKQWPVERFAAAAAELHRTYGAGIVWIATSA
                     EQGKGPKPPAVAPEHCVELVDRPIGEIAAVLRRCTATLANDSGIAHLSSAVGTPAAVV
                     FGPTHPALGFAPRGLCDRVLEVEEYCRPCSLHGEKACFREEPYCLTRISPEAAAEAVA
                     DLIARRQAERPAILADRDGTVMADKEYLGDPAGVELVPGAAESLRAAQARGYRIVILS
                     NQSGVARGLFAIEAVERVNARLRDLLARAGVRADGMYYCPHYEDGAVREYAAACGCRK
                     PAAGMAEQAARELGIDLRRSSVIGDKADDVNLARVMGGRGVLVRTGHGAAEEAKIRAA
                     AGAAGIAVAEDLAAAVKLVMDGDCA"
BASE COUNT          322 a          803 c          665 g          315 t
ORIGIN      
        1 gcggaatgag ttcctccagc ggcgccgcct cgatgtagcc gttggtcacc agcacgactg
       61 cgaggccagc ctgtcggagg atgggcgcca cgtcccgaat gtactcgaac cacatgagcg
      121 gttccgtgta ggtgaacgcc accccgaatg cgccgtagcg gcgggccgcc gcggcgagac
      181 tctccggcga cacgaatttc gtctccgccc gctcctgtga aatcgcccag ttctggcaat
      241 gccggcaccc caggttgcag ctgttcggcc cggtcgagag gatgtcggtc cccggatgaa
      301 aatggtagag tggttttttc tcgatgggat cgagcgcgag cgtgaccgcc tcgccgtagt
      361 tgtccaccag cagctcgccg tcgcgcatga agcggcttcc gcacacgccc cgccgaccct
      421 cgcggaaccg gcagcgcacc gggcagagcg tgcacacgat cccgccgccg ggaagcgcct
      481 cgctgtatgc ggccgccctg atcacgcgca atcaccgtcc atcacaagct ttacggcggc
      541 cgcgagatct tccgcaactg cgatccccgc ggcgccggcc gccgctcgga ttttcgcctc
      601 ttccgccgcc ccgtgcccgg tccgcaccag caccccgcgc ccgcccatca cccgcgccag
      661 gttcacatcg tccgctttgt ccccgatcac cgacgaccgc cgcagatcga tccccagttc
      721 ccgggccgcc tgttccgcca tccccgccgc gggtttgcgg cacccgcacg cggccgcata
      781 ctcccgcacg gcgccgtctt cgtagtgagg gcagtagtac atcccgtccg cccgtacgcc
      841 ggcccgggcg agcaggtcgc ggaggcgcgc gttgacccgc tccaccgcct cgatcgcaaa
      901 cagcccgcgc gccaccccgg actgattgga gaggatcacg atccggtacc cccgtgcctg
      961 cgccgcccgc aaactctccg cggcccccgg gaccagttcc accccggcgg gatcgccgag
     1021 gtactccttg tcggccatca ccgttccgtc gcgatcggcc aggatggccg ggcgctccgc
     1081 ctgccgccgc gcgatcagat ccgcgaccgc ttcggccgcc gcctcgggcg aaatccgcgt
     1141 gaggcagtag ggctcctcgc gaaagcacgc tttttcgccg tgaagggagc acgggcggca
     1201 gtattcctcg acttcgagca cccggtcaca gagtccgcgc ggcgcgaagc cgagggctgg
     1261 gtgggtcggt ccgaagacga cggctgccgg cgtcccgacc gccgatgaga gatgggcgat
     1321 ccccgaatca ttggccagcg tcgccgtgca ccggcgcagg accgcggcga tctcgccgat
     1381 cggccggtcc actagctcca cgcagtgctc gggcgcgacc gcgggcggtt tcggtccttt
     1441 cccctgttcc gcggaggtgg cgatccagac aattccggcc ccgtacgtgc ggtggagctc
     1501 ggccgccgcc gccgcaaatc gctccaccgg ccactgcttc gggcggtggg cggcgcccgg
     1561 cgctgccgcc acgcacacct ggtgccgccc gaggaactcg cgcaccgaag gcggcaggtc
     1621 cgcgtccggc accggcgggg gaaggagcgg gcgccgcgcc ggggttttcc ccccgattgc
     1681 ccgaaccgcc tggttgtaca ggtcgatcgt gtgcggaccg ggcgccagac ggttccgccc
     1741 ccgcagcaac cgccgtcgcg cgagccgccg cttcggatac acggtcgcgt gcggcgccgt
     1801 caccatcttg cgcgtcagcc atgatcggaa attgccgtgc aggtccgcga cggcgtcaaa
     1861 cccccggcgg tccaattcga gcgccagccg caccagctcc cgcccgtccc ctccctccgg
     1921 cagacacacg acctcatcca cgccgtcgaa agcagccgcc aggcccccga accgttcttt
     1981 cgtgaaaaat gtcaggcggc tgcccggaaa actgattttg aggttcagca cggccgccga
     2041 cgtgaggatg acgtcaccca gcgacccgaa ccggatgacc aggatcttct ccatgtcaga
     2101 ggaaa
//
LOCUS       DAOOKS010000356         2105 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_66217_length_2105_cov_2.892195, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000356 DAOOKS010000000
VERSION     DAOOKS010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66217_length_2105_cov_2.892195"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1127
                     /locus_tag="PK112_06275"
     CDS             <1..1127
                     /locus_tag="PK112_06275"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HPC11689.1"
                     /translation="ERPEGPVVWLHAASVGEVKVLSCLYRYLKRRRPHLRACVTVTTT
                     PGHQTARKLFGEEAAVRYLPADTTPIVARVLAAVRPRCLVIAETEIWPILITQAARRR
                     IPVILVNGRMSDTGFRRYRMVAPFLRGLLPCYDRFFFKSEQDAERYRYFGLPESACRV
                     TGDMKFDAPLWPRSAGRRAAVRATVGARDHDWLIVAGSTRPGEEALLLRGIPDLRGAG
                     NGLRLVLAPRHVERAEEVAQACRDAAVPCAVYGSADLSAPVVIVDRMGLLMDLYMAAD
                     LAFVGGTLVDVGGHNILEPVWAGTPVLYGPSLANVGDAAEYIEAHNYGARVGGPEEFA
                     AAVRDIRAGRRTFATKTEHDYDRSPTAAAGEYILAKLSDD"
     gene            1120..>2105
                     /gene="lpxK"
                     /locus_tag="PK112_06280"
     CDS             1120..>2105
                     /gene="lpxK"
                     /locus_tag="PK112_06280"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HPC11690.1"
                     /translation="MIERCWKRFLRRSGMSPWGLLGPPLWLASLLYRFVPGLHRRLTK
                     ERVRLSVPVIAVGNITVGGTGKTPMVHLLARRLIDEGFRIGIVSAGWGRAGDEPILDE
                     GTGLQDRSADDVGDEVLLLAHLVPQAVFSIHPSKSEGARRLAACGERLDAVIVDDAFQ
                     HYRLARDLDLVTYDAALPARVLRPFPYGVMREPRSALRRADIVVITRANFARDIGRLS
                     RRLCKISPRAAHYTAQFSISGLVGAERRYPVKYLEDKSVFLFAGVGNFEPLRRQVSVL
                     AGDLDAALELSDHQRYDAATLARIKRLADRHDSDVIVTTGKDWVKIRHFDFGR"
BASE COUNT          279 a          756 c          731 g          339 t
ORIGIN      
        1 cggagcggcc ggaaggcccc gtcgtctggc tgcacgccgc ctcggtgggc gaggtcaaag
       61 tgctgtcctg cctctaccgc tatctcaaac ggcggcggcc gcacctgcgg gcctgcgtga
      121 ccgtcaccac cacccccggg caccagacgg cgcggaagtt gtttggcgag gaggcggcgg
      181 tgcggtatct gccggcggac accacgccga tcgtcgcgcg cgtgcttgcg gccgtgcggc
      241 cgcgctgcct cgtgatcgcc gagaccgaaa tctggcccat cctcattacg caggcggccc
      301 ggcggcgcat ccccgtcatt ctcgtcaacg gacggatgtc cgacaccggc ttccgccgct
      361 accggatggt cgcccccttc ctccgcggat tgctcccctg ctacgaccgg ttctttttca
      421 agtccgagca ggacgccgag cggtaccgct atttcggcct tcccgagagc gcctgccgcg
      481 tgaccgggga catgaagttc gatgccccgc tgtggccgcg gtcggcggga cggcgggcgg
      541 cggtgcgggc gaccgtcggc gcgcgcgacc acgactggct gattgtcgcg ggttcgacgc
      601 gcccgggcga agaggccctg ctcctccggg ggataccgga cctgcgcggc gcgggcaacg
      661 gtctgagact ggtgctggcc ccgcgccacg tggagcgggc cgaggaggtc gcgcaggcgt
      721 gccgcgacgc cgccgtgccc tgcgccgtct acgggagcgc cgacctgtcg gccccggtcg
      781 tgatcgtcga ccgcatgggc ctgctcatgg atctctacat ggcggccgac ctcgccttcg
      841 tgggcggcac gctggtcgac gtgggcgggc acaatatcct cgagccggtg tgggcgggca
      901 cccccgtcct gtacgggccg tcgctggcca acgtcggcga cgccgccgag tacatcgagg
      961 cgcataacta cggcgcccgg gtcggcggcc ccgaggagtt cgcggcagcc gtgcgcgaca
     1021 tccgggccgg ccggcggact ttcgcaacaa aaaccgaaca cgactacgac cggtcgccga
     1081 ccgccgcggc cggcgagtac atattggcga agctgtccga tgattgagcg atgctggaag
     1141 cgctttctcc gccgctcggg catgtcgccg tggggactgc tcggccctcc cctgtggctg
     1201 gcctcgctcc tctaccggtt tgtccccggc ctgcaccgcc gcctgacgaa ggagcgggtg
     1261 cgcctctccg tgcccgtgat cgcggtgggg aatatcacgg tcggcggaac ggggaagacg
     1321 ccgatggtgc acttgctggc ccggcggctg attgacgagg gcttccgcat cggcatcgtc
     1381 tccgccgggt gggggcgcgc cggtgacgag ccgattctcg acgaggggac cggcctgcag
     1441 gaccgctcgg ccgacgacgt cggcgacgag gttctgcttc tggctcacct ggtcccccag
     1501 gccgtgttct ccatccaccc ctccaagagc gagggggcgc ggcggctcgc cgcgtgcggt
     1561 gagcgtctcg acgccgtcat tgtcgacgac gccttccagc actaccgcct cgcgcgcgac
     1621 ctcgacctcg tgacttacga cgccgccctg cccgcccggg tgttgcgccc ctttccctat
     1681 ggcgtcatgc gcgagccgcg ctcggctctg cggcgcgcgg acatcgtcgt gatcacccgc
     1741 gccaacttcg cccgcgacat cggacggctg agccggcggc tgtgcaaaat cagcccgcgc
     1801 gcggcccact acaccgccca gttctctatc agcgggctgg tcggcgccga gcggcggtac
     1861 ccggtgaagt atctggagga caagtcggtg ttcctgttcg ccggcgtggg caatttcgag
     1921 cccctccggc gccaggtgtc ggtgctcgcc ggcgatctcg acgccgcgct tgaactgtcc
     1981 gaccaccagc gctacgacgc cgcgacgctc gcccggatca agcggctcgc cgaccggcac
     2041 gactcggacg tgatcgtcac gaccggcaag gactgggtga aaatccggca ctttgatttc
     2101 ggccg
//
LOCUS       DAOOKS010000357         2096 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_66652_length_2096_cov_3.299853, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000357 DAOOKS010000000
VERSION     DAOOKS010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66652_length_2096_cov_3.299853"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="PK112_06285"
     CDS             complement(<1..702)
                     /locus_tag="PK112_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11691.1"
                     /translation="MFSRAGAGVLAAAVIFLACSGEYDGAKPAYDTARNPGQFPPPAT
                     ALLDDLEAGRLRGYDETTERFADLYTAHPALLGDPGWSGVIDRLGLRLTWRGDQLVRE
                     GLLQYAAAADLYRLAAQTRPKDSLLSERAELFAAWQEAVGRGAVDPVCLAAPGRGGAA
                     EKLEALTGFALAGARQFRFAEQYLAAALFDRATLDSLRRAEAAGPTAAERALRAALGA
                     GPSPADPPLASFRQPA"
     gene            complement(738..1754)
                     /locus_tag="PK112_06290"
     CDS             complement(738..1754)
                     /locus_tag="PK112_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11692.1"
                     /translation="MSGMHVFEIWIIAFFTLALFSFLYKDNPIYRFAEHIFAGLSAGY
                     YVGLIWHTVILQQLWNPILGGRWLLVIPGLLGALMFARFSSKKAWISRTPLAFVMAVT
                     AGIFLISQLHGLVLPQVQDTMLPKTEAVLVDSTAYEEQADRLVITDSLAAPGAPATAG
                     SVTVSKPEVARTGTDSVVISATFRDPAQPPVERVAFTLKLRLPDDKTQKALVVNATTR
                     TPGVTIAAGGGGVYTASYVYRPDSTRAPGPYDISFQAYRVDHEVMLTLLVLLGVITTL
                     IYFYFSKEHTGALGVAARIGIWFIMIGFGAHFGYTVMGRVSLLIGRVQFLLEDWIGTL
                     TSLF"
     gene            complement(1751..>2096)
                     /locus_tag="PK112_06295"
     CDS             complement(1751..>2096)
                     /locus_tag="PK112_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11693.1"
                     /translation="KNYRDVAFIFEVADNATADYWASIVNAQFGVPMGCGTTAVSAPR
                     YYAFVSSGQFVGLLGGMKGAAEYEMLVGHPGMAYRGMHAQSLVHLVIIGLVVVGNVGF
                     FAGRASRRRGAR"
BASE COUNT          368 a          763 c          677 g          288 t
ORIGIN      
        1 ggcgggttgc cggaaactcg ccagcggcgg gtcggcggga ctcggccccg cgccgagcgc
       61 ggcgcgcagg gcgcgctcgg ccgccgtcgg ccccgccgct tccgcacgcc tcaggctgtc
      121 gagcgtcgcc cggtcaaaca gcgcggcggc gagatactgc tccgcaaacc ggaattgccg
      181 ggcgccggcc agcgcgaaac cggtcagagc ctcgagcttc tcggccgccc cgccccgacc
      241 cggggccgcg agacaaaccg ggtccaccgc cccgcggccg accgcctcct gccacgcggc
      301 gaacagctcg gcgcgctcgg acagcaggct gtctttcggc cgcgtctgcg cggccagccg
      361 gtacaggtcg gccgccgcgg cgtactggag gagcccctcc cgcaccagtt ggtccccccg
      421 ccaggtcagc cggagaccga gccggtcaat caccccggac cagcccgggt cgcccaagag
      481 cgccgggtgg gccgtgtaga gatcggcgaa ccgctctgtg gtctcgtcgt agccgcgcag
      541 ccgccccgcc tcgaggtcgt ccaggagcgc cgtcgccggc ggcgggaatt ggccgggatt
      601 gcgcgccgtg tcgtacgccg gttttgcgcc gtcgtactcc ccgctgcagg cgaggaaaat
      661 caccgccgcc gcgagcacac ccgcgcccgc ccgcgaaaac aaagattctc tcggtcttga
      721 tcgcataacc cgtgatatca gaagagtgaa gtcagcgtcc cgatccagtc ctcgaggagg
      781 aactggaccc gcccgatcag cagcgacacg cgccccatga cggtatagcc gaagtgcgcg
      841 ccgaagccga tcatgataaa ccagatgccg atccgggccg ccaccccgag cgcgcccgtg
      901 tgctccttgg aaaagtagaa atagatcagc gtcgtgatca cacccagcag cacgagcagg
      961 gtgagcatga cctcgtggtc gacgcggtac gcctgaaatg agatgtcgta ggggccgggg
     1021 gcccgggtgg agtccggacg gtagacatag ctggccgtgt acacgccccc gccgcccgcg
     1081 gcgatcgtca cccccggcgt ccgcgtggtc gcgttcacca ccagcgcctt ctgcgtcttg
     1141 tcgtccggga gccgcagctt cagggtgaac gccacccgct cgaccggcgg ctgcgccggg
     1201 tcgcgaaacg tggcgctgat caccacgctg tcggtcccgg tccgtgcgac ctccggcttg
     1261 ctcaccgtca cgctgccggc cgtcgccggc gctccgggcg cggcgaggct gtcggtgatc
     1321 acgagccggt ccgcctgctc ctcgtaggcc gtcgaatcaa ccagaaccgc ctcggtcttg
     1381 ggcagcatgg tgtcctgcac ctgcggcagc acgaggccgt gcagttgcga gatcaggaaa
     1441 atcccggccg taaccgccat cacaaacgcc agcggcgtcc gcgatatcca cgccttcttg
     1501 gacgaaaacc gggcgaacat cagcgccccg agcagacccg ggatcaccag cagccagcgg
     1561 ccgcccaaaa tcgggttcca caactgctgg agaataacgg tgtgccagat cagccccacg
     1621 tagtagccgg ccgacagccc ggcaaaaatg tgctcggcga accggtagat cgggttgtcc
     1681 ttgtagagga aggagaacag cgccagcgtg aagaaggcga tgatccagat ttcgaaaacg
     1741 tgcatcccgc tcatcgcgcg cccctccggc ggctcgcccg gccggcgaag aagccgacgt
     1801 tgccgaccac taccagcccg atgatgacca ggtgcaccag cgactgcgcg tgcatgccgc
     1861 ggtacgccat gccggggtgg cccacgagca tctcgtactc ggccgccccc ttcatcccgc
     1921 cgagcaaccc gacaaactgc ccggacgaga cgaaggcgta gtagcgcggg gcgctcaccg
     1981 ccgtcgtgcc gcaccccatc ggcaccccga actgagcgtt gacgatcgag gcccagtagt
     2041 ccgcggtggc gttgtccgcc acctcaaaaa tgaacgccac gtcccggtaa ttcttc
//
LOCUS       DAOOKS010000358         2095 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_66695_length_2095_cov_3.649020, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000358 DAOOKS010000000
VERSION     DAOOKS010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66695_length_2095_cov_3.649020"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="PK112_06300"
     CDS             complement(<1..389)
                     /locus_tag="PK112_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase gamma subunit"
                     /protein_id="HPC11694.1"
                     /translation="MSGLQLLFYFALAALVVGTGYKVVRIARMPLHLRWDLYPVPHER
                     GKGRYGGSYYEEVDWWTKKVDTTLVGEIREMAGEILALQSLFRHNRQLWIFSFPFHLG
                     LYLLVGFVLLLGLGGAIVASGGAVAPEG"
     gene            1045..1362
                     /locus_tag="PK112_06305"
     CDS             1045..1362
                     /locus_tag="PK112_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HPC11695.1"
                     /translation="MATLTWEEVSISVDEDGFMEEPAKWDERVALALASTEGLTALTD
                     EHWKLITYLRDYYTQYGIAPMIRKLCKETGFPLKKIYELFPSGPAKGACKVAGLAKPT
                     GCV"
     gene            1498..>2095
                     /locus_tag="PK112_06310"
     CDS             1498..>2095
                     /locus_tag="PK112_06310"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPC11696.1"
                     /translation="MIGGAAAGPKTAATLARRRPDWRITLFEKGERVSFATCGLPWLA
                     AGEIPSPDALVRTSWGAERSPEFFRDYKGFTAVTKAEVIALDRARRTVTVRRTATGET
                     FAHGYDKLVLATGATPRTPPFPCCRSERVRHFTRPQDAIAFRRLAEQGKIGEAVIIGA
                     GLVGCELAEAAAGLWGIRTRVIERERHALPYALDEDMAV"
BASE COUNT          386 a          649 c          738 g          322 t
ORIGIN      
        1 ccctcaggcg caaccgcacc gccggaggca acaatcgcgc cgcccagccc gagaagcagg
       61 acgaagccaa cgagcaggta cagcccgagg tggaagggga aggagaagat ccagagctga
      121 cggttgtggc ggaaaagcga ctgaagggcc aggatctccc cggccatctc tcggatctcg
      181 ccgaccaggg tggtgtcgac tttcttcgtc caccagtcga cttcctcata gtaggagcca
      241 ccgtagcgcc ccttgccgcg ttcgtgcggc acggggtaga ggtcccagcg caggtggaga
      301 ggcatgcggg cgatccgaac caccttgtag cccgttccaa cgacaagcgc ggcgagggcg
      361 aagtaaaaca gcagttgcag accgctcatc gggttctcct cagccccccc gggttcggta
      421 atcccgcgcc gctccattca ccgggctcga tcggaagggc tccgctgacc ggggccgggc
      481 cggcgatggc cctggaacct gggggtcagg cggcgaaggg gcggggaaag gtcacattcc
      541 tgtcccggga cgtgtcggga cggccgacga atctgtgaag tgtctctccc gggccgcatt
      601 cggggcgtac cacgggcaca ccgccgggtt ctccgcaaaa aagacggtca aatatagatt
      661 ccctccgcag cgccgtcaag gcgaacttgg ttagctcgcg tccggcgaac cggatcggcc
      721 cgcggtctcg actcaatcag attagcccct cagccacgcg catattatat tgccgggtca
      781 cgaacggccg cacgcccgga cgggtatgcg agaggatgca gcggccgttt cgggcggccg
      841 ggtctcccgc ccggtggcgc ccccggactt cgcgtctgcg gccctgactg cggatggcgg
      901 agacacggtg caggcgccga cggcgatggt gcaaaaagtg tgcggaaggc cgcacgggca
      961 ggtatattgg gaggcgtgca aagcctcgag agcttgaaca aatgatggtg tgcggatata
     1021 attgacaggc agcggaggac gacaatggcg acactcacct gggaagaggt ctcgatttca
     1081 gtcgatgagg atggattcat ggaagagccg gcgaagtggg acgagcgggt tgcgctcgcg
     1141 ctggcgtcaa ccgaaggact gaccgccctg accgatgaac actggaagct gatcacttat
     1201 ctgcgggact actacaccca gtacggcatc gcgccgatga tccgtaagct gtgcaaagaa
     1261 accggctttc cgctaaagaa gatctatgag ctgttcccct cgggtccggc caagggggcc
     1321 tgcaaggtgg ccgggctggc caaaccgacc ggttgtgtgt gagcgcgatc gccgggccct
     1381 cccgcagccg cagcgccggc gagccgggcc ggagagagga gaagactcca ggggtggcgt
     1441 aggccggccc tgacatgcgt ggaaacgata gcagtctctg gacggtcgga tgtcgtcgtg
     1501 atcggcgggg ccgcggcggg tcccaaaacg gccgcgactc tcgcgcggcg ccgcccggac
     1561 tggcggatta ccctgttcga gaagggggag cgggtgtcgt ttgcgacctg cggtctgccg
     1621 tggctggccg ccggagagat cccctcgccc gatgccctcg tccgcacctc ctggggggcg
     1681 gaacgctcgc ctgagttctt ccgcgactac aagggtttca cggcggtgac aaaggccgag
     1741 gtaatcgccc tcgaccgcgc gcgcaggacg gtgacggtgc gccgaacggc gaccggggag
     1801 accttcgcgc acggctacga caagctcgtc ctcgccactg gcgccacgcc gcgcacccct
     1861 ccgtttccct gctgccgaag cgagcgggtg cggcatttca cgcggccgca ggatgcgatc
     1921 gccttccgcc gtctcgcgga acagggcaag atcggggagg cggtgataat cggggcgggt
     1981 ttggtcgggt gtgaactggc ggaggcggcg gccggcctct ggggaatccg cacgcgggtg
     2041 atcgagcgcg aacgccacgc cctgccctat gcgctcgacg aggacatggc ggttc
//
LOCUS       DAOOKS010000359         2091 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_66874_length_2091_cov_3.659627, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000359 DAOOKS010000000
VERSION     DAOOKS010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66874_length_2091_cov_3.659627"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..>2091)
                     /locus_tag="PK112_06315"
     CDS             complement(76..>2091)
                     /locus_tag="PK112_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11697.1"
                     /translation="VSLRAPGRRSLASRLLFIKLKGLDPRRLLERLLPWYRPVHHPLV
                     LASGLAIIAVGLALFVAHAGDFAYRPDRLLQLGSIALIMLTVFMTLVVHEFAHALACH
                     YYGGEVREMGFLLLFFQPCFYADVSDAWLFPRKSHRLTVTWVGPFVQFLLFALAVIVW
                     RVTVPGTFPSRIALIIILVSGISVLFNFNPLIKLDGYYLLSDWLDIPNLRARAFGYLG
                     AWSRRVLLGWPVESVPVPRREKRIYLAYAVLAVLYSGLLIGVILVWASKFLLANFGGW
                     GLLALVVVLTALLKDGLAAAGRGFVQHLKYMSTSLRNPVRVSAYLLAGASMAIGLFAV
                     PFPHRVSGEVTVAPIAEFNLRLNEFGMLERTLIRRGEDPESRSGYMQMVSQDMASLDL
                     TPLVSDGDRVEEGDTLAMLLSNQLSTELVAALAELDRLRRELVLLKSPPKPEAVAEAI
                     AEVNAAQATLAQQERQFQRLRELRERDGISTEQYEAAESALELARAELANRQARLDLL
                     KAGPKPEQEAVVMAQIDKQRARVEFLQTQKDAQSITSPVAGTVRIAPDDGRILSVLNS
                     GTIEVLVPVSDFDIDLVETRRPVLLKVRSYPQRMFLGEVRHIPSAAQSLGGSARFLVS
                     AVVSNEDNALAAGMTGYAKIEIGRISVAAKLGRKIASFLRVEFWSWW"
BASE COUNT          420 a          660 c          717 g          294 t
ORIGIN      
        1 acacgctcgc acaagtgacg ccggaaacta aatctgctgt cgacaataag atcggaggat
       61 aaacctgaag taacactacc accacgacca gaattcaacg cgcaggaaag aggcgatctt
      121 gcgccccagc ttggccgcca ccgagatccg tccgatctcg atcttcgcat acccggtcat
      181 cccggccgcc agcgcattgt cctcgttcga tacgaccgcg gaaaccagaa accgggcgct
      241 gccgccgagc gactgggccg ccgagggaat gtggcggacc tccccgagga acatgcgctg
      301 cgggtacgac cgcactttca gcagcaccgg ccggcgcgtc tccaccaggt cgatgtcgaa
      361 gtccgagacg gggacgagca cctcgatcgt gccgctgttg agaaccgaga gaatgcggcc
      421 gtcgtccggg gcgatacgca cggtgccggc gaccggcgat gtgatcgact gcgcgtcctt
      481 ctgggtctgg aggaactcga cgcgggcgcg ctgcttgtcg atctgggcca tcacgacggc
      541 ctcctgttcg ggcttgggtc cggctttgag caggtcgagg cgggcctggc ggttggccag
      601 ttcggcgcgg gcgagctcga gggcggactc ggccgcctca tactgctcgg tgctgatccc
      661 gtcgcgctcc cggagttctc tcaggcgctg aaattggcgc tcctgctggg cgagcgtcgc
      721 ctgcgcggcg ttcacctcgg cgatggcctc ggcgaccgcc tccggtttgg gcggagactt
      781 cagcagcacc agctcgcgcc tgaggcggtc cagttccgcc agcgcggcca ccagttcggt
      841 gctgagctgg ttcgacaaaa gcatcgccag cgtgtccccc tcctccaccc ggtcgccatc
      901 ggagaccagc ggcgtgaggt caagcgacgc catgtcctgc gaaaccatct gcatgtaccc
      961 cgagcgcgac tcggggtcct cgccgcggcg gatgagcgtc cgctcgagca tgccgaactc
     1021 gttcagccgg agattaaact cggcgatagg ggcgaccgtg acctcgcccg agacccggtg
     1081 cgggaagggc acggcgaaca atccgatcgc catactggca cccgccagca gataggcgga
     1141 cacgcgaacc ggattgcgca gcgaggtact catatacttc aggtgttgca cgaacccgcg
     1201 gccggcggcc gcgaggccgt ctttcagcag cgcggtcagc accacgacca gcgcaagtaa
     1261 cccccacccg ccgaagttgg caagtaagaa cttgctcgcc cagacgagga tcacgccgat
     1321 gagcagcccc gagtagagca ccgcgagcac ggcataggcg aggtagattc gtttctcgcg
     1381 ccggggaacc gggacacttt cgaccggcca gccaagcagc acccgtcggc tccacgcgcc
     1441 gaggtagccg aacgcgcgcg cgcgaaggtt cgggatgtcg agccagtccg acagcaagta
     1501 gtacccgtcg agcttgatca aggggttgaa gttgaacagc acgctgatgc cgctgacgag
     1561 tatgatgatg agggcgatgc ggctgggaaa ggtgccgggg acggtcacgc gccagacgat
     1621 gacggcgagc gcaaagagga gaaactggac gaagggaccg acccaggtga cggtcaggcg
     1681 gtgggacttc cggggaaaca gccaggcatc ggacacgtcg gcgtagaaac acggctggaa
     1741 aaacagcagc aggaacccca tctcgcgcac ctcgccgccg tagtaatgac aggccagggc
     1801 gtgggcaaac tcatggacga cgagcgtcat gaagacggta agcatgatga gagcgatcga
     1861 cccgagctgg aggaggcggt ccgggcggta ggcgaagtcg cccgcgtggg cgacgaagag
     1921 cgccagtccc acggcgatga tcgccaggcc ggacgccagc accagcggat ggtggacggg
     1981 ccggtaccac ggcagaaggc gctccaggag ccgccggggg tcaaggccct tgagcttgat
     2041 gaagagcaga cgggaggcca ggctgcgccg gccgggggcg cggagcgaga c
//
LOCUS       DAOOKS010000360         2090 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_66931_length_2090_cov_3.223587, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000360 DAOOKS010000000
VERSION     DAOOKS010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66931_length_2090_cov_3.223587"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /locus_tag="PK112_06320"
     CDS             complement(<1..735)
                     /locus_tag="PK112_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11698.1"
                     /translation="MKRLATLLVLCLAAAMVAAPAAAAALDRAYARHADTWLDFPAFY
                     AGWDSDDSARCLGASHALHPAVVFSPRGFLGYRFWMAYTPICISESDENPHLAVSNDG
                     REWAEFTAASGCLANPVFDRSLFRAIHLSDADLLLEGDSILYLLFRATWEIAGVDSNA
                     LYASATVDGLVWTPPVKILSDGLLGQPRQSAFVSPALASEGDSVFILWVVEPRADGVS
                     SLDTSRMVEYRAPHPFGPWALVDTCAV"
     gene            1000..1383
                     /locus_tag="PK112_06325"
     CDS             1000..1383
                     /locus_tag="PK112_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018127154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HPC11699.1"
                     /translation="MRKAIKSSRAPKAIGPYSPAIKVAAGTMIFCSGQIPLDPETGTV
                     VGKTAAEQCKQVMENLLGLLADAGVDFSTVVKTTIYLTDMNDFAAVNEVYGSYFEANP
                     PARATVQVARLPKDVKVEIDAIAVV"
     gene            1576..2028
                     /locus_tag="PK112_06330"
     CDS             1576..2028
                     /locus_tag="PK112_06330"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HPC11700.1"
                     /translation="MAEKNTDRRGFLNLILGGSALATLGAIIYPIIKYITPPAVAETD
                     VALKKLEFTQADLEAAEQKQKYFKFGRDLGIVFVTDKGEVRACAATCTHLDCTVQNRP
                     DEGILWCACHNGKYSYDGRNISGPPPRPLAPYRVNIIDGEIIVSKEEA"
BASE COUNT          392 a          611 c          788 g          299 t
ORIGIN      
        1 gaccgcgcac gtgtcgacga gcgcccaggg accgaacggg tggggggcgc ggtactcaac
       61 catgcgcgag gtgtccagag aagagactcc atcggcgcgg ggctccacca cccagaggat
      121 gaaaaccgag tccccctcgg aggcgagcgc gggggagacg aaggccgact gccggggctg
      181 tccgaggagg ccgtcggaga ggatcttgac cggcggcgtc cacaccagac cgtcgaccgt
      241 cgcgcttgcg tacagggcgt tgctgtcgac gccggcgatt tcccaggtgg cgcgaaagag
      301 gaggtacagg atggaatcac cctcgagcag caggtcggcg tcggagaggt ggatagcgcg
      361 gaagagggat cggtcgaaca cggggttggc cagacagccg gaggcggcgg tgaattccgc
      421 ccactcccgg ccgtcattcg atacggcaag gtgcgggttc tcgtccgact cggagatgca
      481 gatgggcgtg taggccatcc agaagcggta gccgaggaag ccgcggggcg agaagaccac
      541 cgccgggtgg agcgcgtgcg aggcgcccag gcagcgggcg ctgtcgtcgg agtcccagcc
      601 ggcatagaaa gcggggaagt caagccaggt gtcggcgtgg cgggcgtatg cccggtcgag
      661 ggccgcggca gccgccgggg cggcgaccat agccgcagcc aggcaaagaa ccagcagggt
      721 ggccaggcgc ttcattactc tctcctgagg gcaggggcgc gcgccctgca gcgttccccc
      781 ggcaactgga ctccggggac tgccgctcgc ggcaacgacc cgccctcgca atggttttca
      841 agtggacgcc tgtaatatag gcacgcgcgc cggaatcggc aaggccgaat ccactccacc
      901 acccctcgcc gaataaccgc ttgggccggg cccgcgaccg cggtatattc gggcggcgaa
      961 aacgacggta tagggtgcga agactcttac ggaggagata tgcgcaaagc gatcaagagc
     1021 agccgggcgc cgaaagcgat cgggccgtac tcgccggcga tcaaggtggc agccggcacg
     1081 atgatattct gctcagggca gattccgctc gatcccgaga cggggacggt ggtcggcaag
     1141 accgcggccg agcagtgcaa gcaggtgatg gagaaccttc tcggcctgct ggccgacgcg
     1201 ggcgtggatt tctccacggt ggtgaagacg accatctacc tgaccgacat gaacgatttc
     1261 gcggccgtca acgaggtcta cggatcgtat ttcgaggcca acccgccggc ccgggccacg
     1321 gtccaggtgg cgcggctgcc gaaggatgtg aaggtggaga tcgacgcgat cgcggtggtg
     1381 tgagggggcg ccgccgcggc gccgatcagg gaccgtttcg atatttattg ttgacatttc
     1441 gcccctcttt cgttttcgtc tccgctgaga cgggcgcggc cgcggccgcc ggcgttcggt
     1501 gaatgtccgg ggttcatggg cggcgcgcag agatggagga gttacgggtg ggccgacagt
     1561 cgggtgaggt gcagcgtggc tgagaaaaac acggaccgtc gcggttttct gaatctgatc
     1621 ctcgggggca gcgccctcgc cacgctcggg gcgatcatct atcccatcat caagtacatc
     1681 acgccgccgg cggtggcgga gacggatgtc gcgctgaaaa agctggagtt cacgcaggcg
     1741 gatctggagg cggcggagca gaagcagaag tatttcaagt tcgggcggga cctggggatc
     1801 gtcttcgtga ccgacaaggg agaagtgcgg gcgtgcgcgg cgacctgcac gcacctggac
     1861 tgcacggtgc agaaccggcc ggacgagggg atcctgtggt gcgcctgcca caacgggaag
     1921 tacagctacg acggccgaaa catctcgggc ccgccgccgc ggccgctggc gccctaccgg
     1981 gtgaacatca tcgacggcga gatcatcgtg agcaaggagg aggcgtgatg gggggcgggc
     2041 ccgggccgaa ggcgcggggg gggcgggggt accgctggct cgacgagcgg
//
LOCUS       DAOOKS010000361         2089 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_66970_length_2089_cov_3.626352, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000361 DAOOKS010000000
VERSION     DAOOKS010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66970_length_2089_cov_3.626352"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="PK112_06335"
     CDS             complement(<1..361)
                     /locus_tag="PK112_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012609951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Ni]"
                     /protein_id="HPC11701.1"
                     /translation="MLRTFVSAALVLGVLAFGSGSAAAHCEIPCGIYDDSTRFDLLAE
                     HITTMEKSMAQIRELSADPGANANQLTRWVVNKEDHAGEFQHIVSQYFMTQRLKTLEP
                     TAQGYAGYQKQLELTHRL"
     gene            complement(651..1040)
                     /locus_tag="PK112_06340"
     CDS             complement(651..1040)
                     /locus_tag="PK112_06340"
                     /inference="COORDINATES: protein motif:HMM:NF021119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein GerW family protein"
                     /protein_id="HPC11702.1"
                     /translation="MSNGVTDILKGVVGELREVAKSETVIGDPITVGDRTVIPVMKIS
                     FGFAAGGGQGTDEKRGSGFGGGGGGGARVEPAAFIIIDRDEIRVLSAVKGKWDALLEA
                     IPEVAKKIQQAAKKFRSDEKPADSSGA"
     gene            complement(1057..1698)
                     /locus_tag="PK112_06345"
     CDS             complement(1057..1698)
                     /locus_tag="PK112_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11703.1"
                     /translation="MPWWPVVIGLLLLLGSGVLALSVRVRLVFEEGRRLIGLSLGRRT
                     GIEVDAGARAGVVRLAGIPLKKFRFGAGAAAPKEPAPAAPRRKRSPRTAGGARRDLAE
                     IGRLGPRILRALGSYLAALSRAATVEECRAHIEAGFASPDHTGLAFGCYQAALGAGPV
                     WLAQIRYVPVWDGPAFRASGRFTAALPLYRLAGRTVLLAWRLPIRKIIRLARA"
     gene            complement(1715..>2089)
                     /locus_tag="PK112_06350"
     CDS             complement(1715..>2089)
                     /locus_tag="PK112_06350"
                     /inference="COORDINATES: protein motif:HMM:NF017745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HPC11704.1"
                     /translation="ENPAMWLAILDGIRFRAARIVADVKLHTGRMTYDECVDWMIDTL
                     DIDTDSGREYIRNQVRLYTHRPTYQMSYLIGKLEILKLRDAMIDIEGESFSLRAFHDA
                     YLSEGSIPPALVWKAWELETAP"
BASE COUNT          366 a          626 c          714 g          383 t
ORIGIN      
        1 gcaggcgatg tgtcagctcg agttgtttct ggtagccggc atagccctgg gcggtcggct
       61 cgagggtttt gaggcgctgg gtcatgaaat actggctgac gatgtgctgg aattcgccgg
      121 catggtcctc tttgttgacg acccaccggg tgagctggtt ggcgttggca cccggatcgg
      181 ccgagagctc gcggatctgc gccatcgact tctccatagt cgtgatgtgt tcggccagca
      241 ggtcgaagcg tgtggagtcg tcatagatcc cgcacgggat ttcacagtgg gcggcggccg
      301 atccggagcc gaatgcaagt acgccgagga cgagagctgc gctgacgaaa gttctcaaca
      361 tgattggttc ctccaaagaa agatcgcgtg ttacagacat ttccatgcat ccaagagggt
      421 aaccgggaga atgcggggag ggtttcctga aagtgcaggt aggtccatga cagaaaaatg
      481 acacgattgg ccgcaaattg tgcggccgag aaaaaaacag tacgaaccaa cgcgggattt
      541 gctctgttta acggggaaag ggatccaacc cttttctcca tttgattgta gccctcgtgc
      601 ttgacggttg cacgggggct atctcttttt ccccatggac ccaggcgcgc ttacgcgccg
      661 cttgagtcgg caggcttctc gtctgacctg aacttcttcg ccgcctgctg gatctttttc
      721 gcgacttcgg ggatcgcctc gaggagggcg tcccactttc ccttgacggc cgagaggaca
      781 cggatctcat cgcggtcgat gatgatgaac gcggccggct cgacccgggc gccgccgccg
      841 ccccccccgc cgaaaccgga gccgcgcttt tcatcggttc cctgtccgcc gccggcggcg
      901 aaaccgaaag agattttcat gaccgggata acggtgcggt cgccgacagt gatcgggtcg
      961 ccgatgacgg tttcggattt ggcgacctcg cggagttcgc cgaccacgcc cttgagaata
     1021 tcggtcacgc cgttcgacat ctcagactcc tttcatctat gctcgggcca acctgattat
     1081 ttttcttatc ggcagcctcc aggcgagcag gacggtgcgc ccggccaatc gatagagcgg
     1141 cagggcggcc gtgaagcggc cggaggcgcg gaacgccggg ccgtcccaga cggggacata
     1201 acggatctgc gccagccaga cgggaccggc gccgagggcg gcctggtagc agccgaaagc
     1261 gaggccggtg tggtcggggc tggcgaagcc ggcctcgatg tgggcgcggc attcctcgac
     1321 ggtcgccgcg cgggagaggg ccgccaggta gctcccgagg gcgcggagaa tccgcgggcc
     1381 gaggcggccg atttccgcca ggtcgcgccg ggctccgcct gcggttcggg gggatcgttt
     1441 ccgccgcggc gcggcgggcg ccggctcttt cggcgcggcg gcgccggcgc cgaaacggaa
     1501 ctttttcagc gggatgccgg ccaggcgaac aacgcccgcg cgggcgccgg cgtcgacttc
     1561 aatgccggtg cggcggccga ggctcagccc gatcagccgc cggccctctt cgaacacgag
     1621 gcgcacgcgc acgctcaggg cgagcacgcc actcccaagg agcagcaaaa gtcctatgac
     1681 caccggccac cagggcatac aacctcgaac cgggctacgg ggcggtttcg agctcccacg
     1741 ctttccacac cagcgcgggc ggaatgctcc cctcgctcag gtaggcgtcg tggaaagcgc
     1801 ggagcgagaa gctctcacct tcaatgtcga tcatcgcgtc gcgcagcttg aggatctcaa
     1861 gtttcccgat caggtacgac atctggtagg tcgggcggtg ggtgtagagg cgcacctggt
     1921 tgcggatgta ctcgcggccg gaatcggtgt cgatgtcgag ggtgtcgatc atccagtcga
     1981 cgcactcgtc gtaggtcatc cggccggtgt ggagcttgac gtcggcgacg atgcgggcgg
     2041 cgcggaagcg gatgccgtcg aggatggcga gccacatggc ggggttttc
//
LOCUS       DAOOKS010000362         2087 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_67059_length_2087_cov_2.681594, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000362 DAOOKS010000000
VERSION     DAOOKS010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67059_length_2087_cov_2.681594"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            222..>2087
                     /locus_tag="PK112_06355"
     CDS             222..>2087
                     /locus_tag="PK112_06355"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPC11705.1"
                     /translation="MKEYPTDKIRNICLAGQRGCGKTSLADALAYATGVNNRIGRVDD
                     GSSLLDYTDSEISRRTSIQLKLLAMAWKDCKLNLFDAPGHLDFMGEFLACAKVSDVVC
                     FVVNATAGVEVGTQLQWRSLADSPHARFIFVNKMEIENAKWQTTVESIQAAFGNHAVP
                     VTIPIGEADKFRGMIDLLHMKAYEYAPDGTRKEIDIPADLKDRAQADREALVEMAAEA
                     SDELMEKFFDQGTLPEEDVRIGLRQGIARGKLYPILCGAAGKNWGTGVLLDFAVKFLP
                     SPDQMPAPQAQKTGAADLVEVRCDPNGPPALFVFKTITEGHLGELAFFRVYSGAVSSG
                     MDFVNQRTRNSERATQIYTQQGKNRSELSKVVAGDVGALVKLRNTNTGDTLTGGNLSL
                     VIPPVRYPNPVMDVAIRPKSKGDEEKIGTGLQKISQEDPTFRIIHDAALKQMVLVGQG
                     ATQIEIITEKLKKRYGVEVDLVRPRIPYRETIRAKAETQYRHKKQSGGRGQFGDVHLR
                     LEPNERGKGFEFLDEIKGGVIPNKYIPAVEKGVVEAMERGPLTGSQVIDVKVAVFYGS
                     YHEVDSSDMAFKIASSMAFKQGFMQAKPVLLEPIYNVEVLVPEDFTGDVMGDLSAR"
BASE COUNT          431 a          618 c          693 g          345 t
ORIGIN      
        1 gggcttcggc tattaccaca aacccccgcc accgccaccg cgccggcgcg cctgtccgcg
       61 cccttccccg ccggccgatt tcccgggcgg gacggccaaa ggcgttgact tttcagcccc
      121 tgtttggtat gtatggagtt cgcggtttcg ggcggaaagc ccgcaacgga aagaacggac
      181 ggaacataga atttatgata cgatagtttg gagggtccct ggtgaaggaa tatccgaccg
      241 acaaaatccg taacatctgc cttgccgggc agcggggatg cggcaagacc agcctggccg
      301 atgcgctggc gtacgccaca ggcgtaaaca accggatcgg gcgggtcgac gacggttcct
      361 cgctgctgga ttacaccgac agcgaaattt cgcgccggac atcgattcag ctgaagctgc
      421 tggcgatggc gtggaaagac tgcaaactca atctgttcga cgccccgggg cacctggact
      481 tcatgggcga attcctcgcc tgcgccaaag tctccgacgt cgtctgtttc gtcgtgaacg
      541 ccaccgccgg cgtggaagtc gggacacaac tgcagtggcg gtcgctcgcc gattccccgc
      601 atgcccgctt cattttcgtc aacaagatgg agatcgagaa cgccaagtgg cagacgaccg
      661 tggagtcgat ccaggcggcc ttcggcaacc acgccgtgcc ggtgactatc ccgatcgggg
      721 aggcggacaa attccggggc atgatcgacc tgctgcacat gaaggcgtac gagtatgcgc
      781 cggacgggac gcgcaaggag atcgatattc cggctgacct gaaggaccgg gctcaggccg
      841 atcgcgaagc gctcgtcgag atggcggccg aagccagcga cgagctgatg gaaaagtttt
      901 tcgaccaggg gacgctaccc gaagaggatg tccggatagg gctgcggcag gggattgcgc
      961 gcggcaagct ctaccccatt ctctgcggcg cggcgggcaa gaactggggc accggggtgc
     1021 tcctggattt cgcggtcaag ttcctgccgt cgccggacca gatgccggcg ccgcaggccc
     1081 agaagaccgg cgccgccgat ttggtcgagg tgcggtgcga tccgaacggc ccgccggcgc
     1141 tgttcgtctt caagacgatc accgaaggcc acctcgggga actggcgttt ttcagagtgt
     1201 actcgggagc ggtctcctcg ggcatggatt tcgtcaacca gcggacgcgc aactccgagc
     1261 gggccaccca gatatacacg cagcagggga agaaccgatc ggagctgagc aaagttgtgg
     1321 ccggggacgt cggagcgctg gtaaaactgc gcaacaccaa taccggcgat acgcttacag
     1381 gcggcaatct cagcctcgtc attccgccgg tgcgctaccc caacccggtg atggacgtgg
     1441 cgatcaggcc caagtccaaa ggggatgaag agaagatcgg gaccggcctg cagaaaatca
     1501 gccaggagga tccgacgttc aggatcatcc acgacgccgc tctcaagcaa atggttctcg
     1561 tcgggcaggg ggccacccag atcgagatta tcaccgagaa gctgaagaag cggtacgggg
     1621 tggaagtcga cctggtgcgg ccgcgcatcc cctaccggga gacgatccgg gcgaaggcgg
     1681 agacgcagta ccgccacaag aagcagtcgg gcgggcgggg gcagttcggc gacgtacacc
     1741 tgcggctgga gcccaacgag cgcggcaagg ggttcgagtt cctcgacgag atcaagggcg
     1801 gggtgatccc gaacaagtac atcccggcgg tcgaaaaggg cgtggtcgag gcgatggagc
     1861 gcgggccgct gaccggctct caggtgatcg acgtgaaggt ggcggtcttc tacggttcgt
     1921 accacgaagt cgattcgtcg gatatggcat tcaagatcgc ctcctcgatg gccttcaaac
     1981 agggcttcat gcaggccaag ccggttctgc tcgagccgat ctacaacgtc gaggtgctag
     2041 tccccgagga tttcaccggc gacgtcatgg gcgatctctc ggcgcgg
//
LOCUS       DAOOKS010000363         2085 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_67126_length_2085_cov_3.706897, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000363 DAOOKS010000000
VERSION     DAOOKS010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67126_length_2085_cov_3.706897"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            123..1256
                     /locus_tag="PK112_06360"
     CDS             123..1256
                     /locus_tag="PK112_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11706.1"
                     /translation="MDQPLYYDFRDILIAPARALAAKRIFVMTLFLCLAVAIYDVFVY
                     LAAVLDDASLETFWRWHGLLPLAGLNFSGGLAQAACGLGIVLALLALMTGLFAVSAIE
                     IEQLRGNRFFPMCEAIRFARRRLGQLAAGEAAMVAFLLFILLLFLLLGLVVRIPAVGE
                     WVYAATFLLPVFFIAIFTVFIVAVLQISVILLPAAAAAERHGESFTAILGTFSTVIRQ
                     PVRWLAYTAYGLAAAKAASWIYAYFCYRAVQFTAWAASLTAGEKADRLVREGLGHLPV
                     RSGPVEAAVALVPGFDWRFSISQWAERGDSAAGYVMAAMLLVILASVFGYALAVVATT
                     QARAYAVIRFLKDDYRIAEEPPLFLPEAREDETGSGGAGAPAG"
     gene            1389..>2085
                     /locus_tag="PK112_06365"
     CDS             1389..>2085
                     /locus_tag="PK112_06365"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
                     /protein_id="HPC11707.1"
                     /translation="MTAEKKILVTGVTGAQGGSVARHLLRRGRFAVRGLTRHPESPQA
                     KDLARRGVEIAAGDMGDPQSLVRAMDGCWGVFGVTNFWEHFHREFELGRNLIRAVAET
                     RPEFFIYSSLPNPRKLGRGEYDVPHLELKARLEDEIRALPLRSAFTHVAFYYENYMIF
                     SMLQRAEDGSLSFGFPQGETKLAMVGTEDIGGVVAPLFERPDEFAGRQVGVVGDDRPP
                     AEYAQALARALEVP"
BASE COUNT          280 a          687 c          748 g          370 t
ORIGIN      
        1 tggacgacga cgtgaacgaa ctgcccgacc ggccgaaggt ggacgagtag ccgggcgggg
       61 cgcggccgcc ccggcccgct atttagcttg acaccctgcg ccgcgcgcgg cacccttccg
      121 gtatggacca gccgctgtat tacgacttcc gggacatcct catcgcgccg gcccgggcgc
      181 tcgccgccaa acgcattttc gtcatgacgc tgttcctgtg cctcgccgtc gccatctacg
      241 acgtgttcgt ctacctcgcg gcggtgctcg acgacgcctc gctcgagacc ttctggcggt
      301 ggcacgggct tttgccgctc gccgggctga atttcagcgg cggcttggcg caggccgcct
      361 gcggactggg gatcgtgctg gcgctgctgg cgctcatgac ggggttgttc gccgtgagcg
      421 cgatcgagat cgaacaattg cgggggaacc ggttcttccc gatgtgcgag gcgatccggt
      481 tcgcgcgccg gcggctgggc cagctggcgg ccggcgaggc ggccatggtt gccttcctcc
      541 tgttcatcct cctgctcttt ctgctgctcg ggctggtcgt tcgcatcccg gccgtcgggg
      601 agtgggtgta cgcggcgacc tttctcctgc cggtcttctt catcgccatc ttcaccgtgt
      661 tcatcgtcgc cgttctccaa atctccgtga tcctgctccc cgcggcggcc gccgccgaac
      721 gccacggcga gtcgttcacc gcgatcctcg ggacgttttc gaccgtcatc cgtcagccgg
      781 tccgctggct ggcctacacc gcctacgggc tggccgcggc caaagcggcg agctggatct
      841 atgcctactt ctgctaccgg gcggtgcagt tcacggcgtg ggccgcgtcg ctcacggccg
      901 gggagaaggc ggaccggttg gtccgcgagg ggctggggca tctgccggtg cgctcgggcc
      961 cggtcgaggc ggcggtcgcg ctcgtgccgg ggttcgactg gcggttttcg atctcgcagt
     1021 gggccgaacg gggggacagc gccgccggat acgttatggc ggccatgctc cttgtcatcc
     1081 tcgcctcagt tttcggctac gccctggcgg tggtggcgac gacgcaggcg cgcgcctacg
     1141 ccgtcatccg gtttctcaag gacgactacc ggatcgccga ggaaccgcca ctctttcttc
     1201 ccgaagcgcg ggaggacgag accgggtcgg gcggcgcggg ggcgccggcg ggctgagcgg
     1261 cgcggcttcc ccgccgcgcc atctttcggc ttgactcatc gagccataat gtccatattg
     1321 ccgcgtccgg gaacgccggg gcggggaaga aacgatcgcg tggaccccac aacgatgcgg
     1381 aggatgcagt gacggcagag aagaagattc tggtaaccgg ggtgaccggg gcgcaggggg
     1441 gcagcgtggc gaggcacctc ctgcggcggg ggcggttcgc ggtgcgcggg ctgacgcgcc
     1501 atcccgagtc gccgcaggca aaggacctgg cgcgccgggg cgtcgaaatc gccgccggcg
     1561 acatgggcga cccgcagagt ctggtgcggg cgatggacgg gtgctggggc gtgttcggcg
     1621 tcaccaactt ctgggagcac ttccaccgcg agttcgaact cgggcgcaac ctgatccggg
     1681 cggtcgcgga aaccaggccc gaattcttca tctacagctc cctgcccaat ccgcggaaac
     1741 tcggccgggg ggagtacgac gtgccgcacc ttgagctcaa ggcgcgcctg gaagatgaga
     1801 tccgcgccct gcccctccgg tcggccttca cccacgtggc gttctactac gaaaactaca
     1861 tgatcttctc catgctccag cgggccgagg acgggtcttt gtccttcgga tttccgcagg
     1921 gcgagacgaa gctggcgatg gtgggcacgg aagatattgg gggcgtggtg gccccgctgt
     1981 tcgagcgccc ggacgagttc gccgggcggc aggtgggggt ggtgggggac gaccggccgc
     2041 cggccgagta cgcccaggcg ctcgcgcggg cactcgaggt cccgg
//
LOCUS       DAOOKS010000364         2082 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_67262_length_2082_cov_3.435126, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000364 DAOOKS010000000
VERSION     DAOOKS010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67262_length_2082_cov_3.435126"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(366..1337)
                     /locus_tag="PK112_06370"
     CDS             complement(366..1337)
                     /locus_tag="PK112_06370"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPC11708.1"
                     /translation="MKYLSRMILLLPAAFVCLAGRPAAQDSLKIYLELGGKAIQNSKY
                     DRAIEVYTRVLQLDSANYEAISNLGVAYASLGNYARARGCLEQALRRNPGDAQAANNL
                     GTVFSNENDRTAAIEYFEKAVGLAPKNALFLTNLGREYARDGQVGKALPVLREALVLA
                     PDNPLIPYSLGSAFASAGSLDSAEVYFERSLAKGGTGVELHYFLGTVKDRLGKKDEAV
                     NHYKSALEMEPDHLNCLQSLGLIYLSQDRFPEAIEQFRRVIAVDSTQWAGWIGLGASY
                     SLYRMIPQADSILGRLFQADSAIGFQMLDFIGQQHRLRRDSTGSKTR"
     gene            1628..>2082
                     /locus_tag="PK112_06375"
     CDS             1628..>2082
                     /locus_tag="PK112_06375"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPC11709.1"
                     /translation="MSTETTSIRKAHRLVHGEGATILCLHASTSSSKQWQPLAERMAA
                     RYRVVAADLYGYGQSPEWHGERPLTLDDEISLIADEIAAADEPVHLVGHSYGGAVAAR
                     VAVAYPDRVASLTLYEPVMFGLLRDDPNSRAAWEEIRAVERFVRTRTAAG"
BASE COUNT          390 a          661 c          694 g          337 t
ORIGIN      
        1 gtcggccgcg tgcataaagt cggagatcgt ctccggacgc ggcgccggat ccgccccggc
       61 cagggtgtcc ggccctccgt tgaaccaatc ctgggcagcg gccggcgaac acaggagcac
      121 caggccggcg agacacaacg cccgaacgcg tgcgtgcata cggtctcctt tcgaaacaaa
      181 atccatgtcc aagcgggatt caaacggtgt gccggggcgc cacgggtcaa aggacaaggg
      241 ggcagcccgg ccgggcccgc ccggatgtgg gcgcgggggc acacagtatg gggggagagc
      301 aaggcgtttg gatagtgctg tcggcggagc ggaaccggcg cgacccggcc gccccggcgc
      361 cgcggctagc gcgtcttgct gccggtgctg tcgcgccgga ggcgatgctg ctggccgatg
      421 aaatcgagca tctgaaaccc gatcgcggag tcggcctgga acagccgccc gagaatgctg
      481 tcggcctggg ggatcatgcg gtacagcgaa tagctggcgc cgaggccgat ccagccggcc
      541 cattgagtcg agtcgaccgc aatcacccgg cgaaactgct cgatcgcctc cggaaaccga
      601 tcctggctga gatagatcag accgagcgac tggagacaat tgagatggtc aggctccatt
      661 tccagcgcgc tcttgtagtg attgaccgcc tcatccttct tgcccaggcg gtccttgacc
      721 gtgccgagga agtagtgcaa ttcgacgccc gtgccgcctt tggcaaggct gcgctcaaaa
      781 tagacctcgg cgctgtcgag ggaacccgcg gaggcgaaag ccgaccccag cgagtaggga
      841 atcagcgggt tgtcgggtgc caggacgaga gcctcccgaa gaaccggaag tgctttgccg
      901 acctggccat cgcgtgcata ttcccgtccc aagttggtca ggaacagcgc gtttttcgga
      961 gccagcccga ccgccttctc gaaatactcg atcgcggccg tccggtcgtt ctcgttgctg
     1021 aacacggtgc cgagattgtt ggcggcctgc gcatcgcccg ggttgcgccg gagcgcctgc
     1081 tccagacacc cccgggcccg cgcatagttg cccagactgg cgtaggccac ccccagattc
     1141 gagatcgcct catagttggc cgagtcgagc tggaggacgc gcgtgtagac ctcgatcgcg
     1201 cggtcatact tgctgttctg aatcgccttg ccgcccagct ccagataaat cttcagcgag
     1261 tcttgcgccg ccgggcggcc cgccaggcac acgaacgcgg ccggcagcag gaggatcatt
     1321 ctcgaaagat atttcatatg ggcttataca atacaaccgc accgcgtgtc cgtcaagcgc
     1381 cgaagatccc ctggggggac gaagcggggc ctcgggcaaa gagttacggc cgccggcggc
     1441 cggggcggga gtccgcagca gaaaaataac cggaaccgga cgaaacaagt cttgtttttc
     1501 ttcgtaccgg gccgtattgt ggatagattt tatctgagat cgtcaccggc ccctgcggcg
     1561 gccgctccgg ccggaccggg acaaaccgcg ccgccaagat agatgacggg aaatccagaa
     1621 agggagtatg agcacagaaa ctacttcgat acggaaagcc catcgcctgg tccacggcga
     1681 gggcgcaacc atcctctgcc tgcacgccag cacgagttcc tccaagcagt ggcagccgct
     1741 cgccgagcgg atggccgccc ggtatcgggt cgtggccgcc gacctgtacg gctacggcca
     1801 gtcgcccgag tggcatggtg agcggccgct gacgctcgac gatgagattt cgctcatcgc
     1861 cgatgaaatc gcggccgccg atgaaccggt gcacctggtg gggcactcct acggcggcgc
     1921 cgtcgcggcc cgggtggcgg tcgcctaccc cgacagggtg gcgagcctga cgctgtacga
     1981 gccggtgatg ttcggtcttc tgcgcgatga tccgaattcg cgggccgcgt gggaggagat
     2041 ccgcgcggtc gaacggttcg tccgaacccg caccgcggcc gg
//
LOCUS       DAOOKS010000365         2077 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_67507_length_2077_cov_3.167656, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000365 DAOOKS010000000
VERSION     DAOOKS010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67507_length_2077_cov_3.167656"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="PK112_06380"
     CDS             complement(<1..309)
                     /locus_tag="PK112_06380"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HPC11710.1"
                     /translation="MDALYPIRVVAAKTGLSAHLIRMWERRYGAVEPSRTNTRRRLYS
                     ESDVRRLTLLRRATQAGAAIGQIATLPEDDLESLVASYEQLGAVRGAGAPGPPAPGYF
                     V"
     gene            complement(329..775)
                     /locus_tag="PK112_06385"
     CDS             complement(329..775)
                     /locus_tag="PK112_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11711.1"
                     /translation="MRKFVSVARVSPALDSAVLNQPQFAQPARRRIAHSAERLPHKLP
                     LRKLLLRKRKKIGDKRQIVAIVFGWPPVSRRGILPHVIRPIFRRDTSNTGDFIPSTHR
                     QYMSFSPQAGRKFTMPAPQHPTCAEKPEWKIVNFLSLASVIQYDKY"
     gene            854..1588
                     /locus_tag="PK112_06390"
     CDS             854..1588
                     /locus_tag="PK112_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11712.1"
                     /translation="MEALIAGVAGRRAGGELPPAADRAASRVTERGAVSIDDTSRLFA
                     GVGGADGAWAELMRSLAVLPMRVDRDRGGVLVLGSRETWHFGPETMGCLEEAAAVIGA
                     ALAHREARRDLRERVKEMTCLYGMAQVAANPQVSSEEFLEKCIELLPAAFRHPLAARA
                     RITFDGRTAGAAGFPERGARLAAPIRFGGRQRGLVEVAYAEDLPPRDLGPFLREEETL
                     LQTVAENVAVILEQRQASAEVHGLLD"
     gene            1625..>2077
                     /locus_tag="PK112_06395"
     CDS             1625..>2077
                     /locus_tag="PK112_06395"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HPC11713.1"
                     /translation="MTAGVVHELNEPLGGILGFAQLAKKAPGLTGQTRQDIDKIIAAA
                     LHAREVVRRLMLFARRLPPRKNRVNLNDVITEGLYFLESRCERLGIALERELQPDLPE
                     LAGDASQLYQVLVNLVVNAIQAMPEGGFITIRTEATAAAVILSVADTGV"
BASE COUNT          336 a          551 c          843 g          347 t
ORIGIN      
        1 cacaaagtag ccgggtgcgg gcggtccggg agccccggcg ccgcgcacgg cgccgagctg
       61 ttcgtaggag gcgacgaggc tctcgaggtc gtcctccggg agggtggcga tctggccgat
      121 ggcggcgccg gcctgggtgg cgcgccgcag cagggtgagc cggcggacat cgctttcgga
      181 gtaaaggcgg cggcgggtgt tggtgcggga gggttcgacc gcgccgtagc gccgctccca
      241 catgcggatg aggtgggcgg aaaggccggt cttggcagcg accacacgta tggggtagag
      301 cgcgtccatc agtctatcta agtattgtct aatatttgtc atattggata acagaggcaa
      361 gggataaaaa gttcacgatc ttccattccg gtttttctgc gcaggtcggg tgttggggcg
      421 caggcattgt gaattttctc cccgcctgag gagagaatga catatattgg cggtgtgtac
      481 tggggataaa atccccggtg tttgatgtat cacggcggaa aatggggcgg atcacatgag
      541 gcaaaatacc ccgccgggag acaggcggcc atccgaaaac gatcgcaact atttgacgct
      601 tatcgccaat cttcttgcgt tttcgcaaca ggagctttct caaggggagt ttgtgcggga
      661 ggcgctcagc ggagtgtgcg atgcggcggc gtgcgggctg ggcgaattgc ggttggttaa
      721 ggacggcgct gtcgagcgct ggcgatacac gcgcgacaga gacgaattta cgcacgagcg
      781 gggtccggca gcggaggacg agggagcggg agatcatgct gggggagacg cgcgggcgga
      841 atgcgacgct cctttggaag cccttatcgc cggcgtggca gggcgccggg cggggggtga
      901 actgcctccg gcggccgacc gggcggcctc gcgggtgacc gagcggggag ccgtgagtat
      961 cgatgatacc tcgcggctgt tcgcgggtgt cgggggagcg gacggggcct gggcggagct
     1021 tatgcggtcg ctggcggtgc tgccgatgcg ggttgaccgc gaccggggcg gagtgctggt
     1081 gctgggttcg cgggagacct ggcactttgg acccgagacg atgggttgtc tggaggaggc
     1141 ggcggcggtg atcggggcgg cgctggcgca ccgggaggcg cggcgcgatc tgcgcgagcg
     1201 ggtcaaagag atgacgtgcc tgtacggaat ggcgcaggtg gcggcgaacc cgcaggtgtc
     1261 gtcggaggaa tttctcgaga agtgtatcga actgctgccg gcggcctttc ggcacccgct
     1321 ggcggcgcgc gcgcggatca cgttcgacgg gcggacggcg ggggctgcgg gatttcccga
     1381 gcggggagcg cggttggccg cgccgatccg gttcggcggc cggcagcggg ggctggtcga
     1441 ggtggcctac gccgaggatc ttcccccccg cgacctcgga ccgtttctcc gggaggagga
     1501 gacgctgctg cagacggtgg cggagaacgt ggcggtgatt ctggagcagc ggcaggcctc
     1561 ggcggaagtg cacgggcttt tggattagct tcgccacgcg gaccggctgg cgacgatcgg
     1621 gcaggtgacg gcgggggtgg tgcacgagct gaacgagccg ctcgggggga tactggggtt
     1681 cgcgcaactg gcgaagaagg cgccggggct gaccgggcag acgcggcagg atatcgacaa
     1741 aatcatcgcg gcggcgctgc acgcgcgcga ggtggtgcgg cggctcatgc tgttcgcgcg
     1801 gcgtctgccg ccgcgcaaga accgggtcaa cctgaacgat gtgatcacgg aggggctgta
     1861 tttcctcgaa tcgcgctgcg agcggctggg gatcgccctg gagcgggaac tgcagccgga
     1921 cctcccggag ctcgcggggg atgcgagcca gttgtaccag gtgctggtca acctggtggt
     1981 gaacgcgatc caggcgatgc cggagggcgg gttcatcacg atccggaccg aggcgacggc
     2041 ggcggcggtc atcctgagcg tcgccgacac cggggtg
//
LOCUS       DAOOKS010000366         2077 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_67511_length_2077_cov_2.847676, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000366 DAOOKS010000000
VERSION     DAOOKS010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67511_length_2077_cov_2.847676"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..>2077)
                     /locus_tag="PK112_06400"
     CDS             complement(80..>2077)
                     /locus_tag="PK112_06400"
                     /inference="COORDINATES: protein motif:HMM:NF016563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl hydrolase"
                     /protein_id="HPC11714.1"
                     /translation="FDVAAHEDKEARLVIVDAESGPWGNIGVDHIVFCDGAPGEPYRL
                     REQHDWGTMSLAVVGGGERVSGCAQVATAESFCAAPDCAAREARAPFGERLLGAVGRA
                     FTLEPGREESVVFIAAWHFPNLYLPGLPGNLGRYYATRAASSFEVAAAVAGDLENLTA
                     LTELWHRTWYRDSTLPWWFLNRTFVNTSILATDTAYRFANGRFYGWEGIDCCPGTCGH
                     VWMYAQAVGRLFPELERDLRERVDLDLAMQPSGAVHFRAEHNRSVAVDGQAGVVLRAY
                     REHQMTADAAFLARNWPAIARALSYLIRQDGDGDGLLEGAQHNTLDADWYGRIPAMSS
                     LYLAALKAGNAMAEEVGDAEFAALTARILAAGRKNILALFNGRYFIQVEDPAYPDAIG
                     VGAGCHIDNVFGQSWAFQVGLGRIIDAGPVRTALDSLWKFNFAPDVGPLRASLPDKIK
                     GRPYALAGEAGLLMCTWPLGGGRADRQRHWQYGYFNECMTGFEYQVAGHMLWEGMVER
                     GLAVTRAVHDRYAPAKRNPYNEIECSDHYARAMASYGVFLAACGYEHHGPKGSLAFAP
                     RLSPEDFSAAFTAAGGWGLFAQKRAGAAQRDTLELRWGSLTLRSFAFEAPGAVDAEKV
                     RAELDGRPIECEAARDGARILVTFPRPVVLRAPAKLVIDAG"
BASE COUNT          332 a          753 c          699 g          293 t
ORIGIN      
        1 ccaaacgccg cctccatgat gccccggccg aagcccgaag acaatcgccc gccggcaacg
       61 ctgccgcccg cccgcggcct caaccggcgt cgatcacgag cttcgccggc gcccgcagca
      121 cgacgggccg cgggaaggtg acgaggatgc gggcgccgtc gcgcgccgcc tcgcattcga
      181 tcggccggcc gtccagctcc gcgcggacct tctcggcgtc aacggccccc ggcgcctcga
      241 aagcgaaact ccgcagcgtg agcgatcccc agcgcagctc gagcgtatcg cgctgcgccg
      301 cgcccgcgcg cttctgcgcg aagagccccc agcccccggc ggcggtgaac gccgcgctga
      361 agtcctcggg cgagagccgc ggcgcgaagg ccaggctgcc tttgggaccg tggtgctcgt
      421 acccgcaggc cgccaggaag acgccgtagc tggccatggc ccgggcgtag tggtcgctgc
      481 actcgatttc gttgtaggga ttgcgcttcg ccggggcgta gcggtcgtga acggcgcgcg
      541 tcacggccag gccgcgctcg accatccctt cccagagcat gtgccccgcc acctggtatt
      601 cgaacccggt catgcattcg ttgaagtagc cgtactgcca gtggcgctgc ctgtcggcgc
      661 gcccgccgcc gaggggccag gtgcacatga ggagcccggc ctcgccggcc agggcatagg
      721 gccggccctt gatcttgtcc ggaagcgagg cgcgcagggg gcccacgtcc ggcgcgaagt
      781 tgaacttcca gagcgagtcg agcgccgtcc tgaccgggcc cgcatcgatg atccgtccca
      841 agcccacctg aaaggcccag ctctggccga agacgttgtc gatgtggcag cccgcgccca
      901 cgccgatcgc gtcgggatag gcgggatcct cgacctggat gaagtaccgg ccgttgaaga
      961 gcgccaggat gttcttgcgg cccgcggcga gaatgcgcgc cgtcagcgcc gcgaattcgg
     1021 cgtcgccgac ctcctcggcc atggcgttcc cggccttgag cgccgccaga taaagcgagc
     1081 tcatggccgg aatgcggccg taccaatcgg catcgagcgt gttgtgttgc gcgccctcca
     1141 gcaacccatc gccgtcccca tcctggcgga tcaaataact cagggcgcgg gcgatggcgg
     1201 gccagtttct ggcgaggaag gcggcgtcgg ccgtcatctg gtgttcgcgg taggcgcgca
     1261 ggaccacgcc ggcctggccg tccaccgcca cggaccgatt gtgctcggcg cggaaatgca
     1321 cggcgcccga gggctgcatc gccagatcca ggtccacgcg ctccctcagg tcccgctcca
     1381 actccggaaa gagcctcccg acggcttggg cgtacatcca cacgtggccg caggtgcccg
     1441 ggcagcagtc gatgccttcc cacccgtaga agcgtccgtt cgcgaagcgg tacgccgtgt
     1501 cggtggccag aatcgacgtg ttgacgaaag tccggttcaa gaaccaccag ggcagggtcg
     1561 aatcgcgata ccaggtccgg tgccagagct ccgtcaacgc cgtcaggttc tccagatcgc
     1621 ccgccaccgc cgcggcgacc tcgaacgacg aggcggcgcg cgtcgcgtag taccggccca
     1681 gattgccggg caggcccgga agatacaggt tcgggaaatg ccacgccgcg atgaagacga
     1741 cggactcctc gcgtccgggc tcgagggtga aggcgcgccc caccgcgccc aggaggcgct
     1801 cgccgaaggg cgcccgcgcc tcgcgcgcgg cgcaatccgg cgcggcgcag aagctttccg
     1861 ccgtggcgac ctgcgcgcag ccggagaccc gctctcctcc gccgacgacc gccaggctca
     1921 tggtgcccca gtcgtgttgc tcccgaaggc ggtagggttc gccgggggcg ccatcgcaaa
     1981 aaacgatgtg atcgacgccg atgttccccc acggcccgct ctccgcatcg acgatgacaa
     2041 ggcgggcctc cttatcttcg tgcgccgcga catcaaa
//
LOCUS       DAOOKS010000367         2075 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_67623_length_2075_cov_2.902475, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000367 DAOOKS010000000
VERSION     DAOOKS010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67623_length_2075_cov_2.902475"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..861)
                     /locus_tag="PK112_06405"
     CDS             complement(<1..861)
                     /locus_tag="PK112_06405"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
                     /protein_id="HPC11715.1"
                     /translation="MHRASLFVWVAALAVCPLAAVSSGDVCGDVNGDGSVSVADVTGA
                     VNYLFRGIPEQPYFRPADVNGDYGVTVADLTWLVSYLFRGGPALECPATFTPAMGDGC
                     VEAWTSTAGGQPNPAVRSSGCLTVAPDDSAEYMFVELVGRDLHVHHVNAYYQCCLGYA
                     ATFEIDGRSVTVQEADTSFFPCPCMCYFNLEAILPGFAAAEPGEYAVTLVGLEGDTVG
                     ADTLVIGGPETMSLEVVGKDLRVRHLNAQANCCPAFLTDVFVNGGAITVIEWDTLSAC
                     EIEGAGDSTIR"
     gene            complement(999..1793)
                     /locus_tag="PK112_06410"
     CDS             complement(999..1793)
                     /locus_tag="PK112_06410"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HPC11716.1"
                     /translation="MELSARDLSRFQRFAEDTARLAGELLKKRFHAAHRVRYKGRIDP
                     VTEADVKSERLITARLRRAFPGHALLAEEGSGTGAAAEFQWVVDPLDGTVNYAHGFPI
                     YCVSIALQFRGLPAAGAVYDPERDEMFRGARGQGAYCNRTRLRVAREIDLQRALLATG
                     FGYNVATARRNNLGYFARMVKRAQGVRRPGSAAIDLCWLAAGRIDGFWELDLHPWDTA
                     AAVLFIEEAGGKVTRTDGRPFAITDRSILAANPRLHASMLKVLSGR"
     gene            complement(1799..>2075)
                     /locus_tag="PK112_06415"
     CDS             complement(1799..>2075)
                     /locus_tag="PK112_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPC11717.1"
                     /translation="DEATSSVDTETEHLIQQALEELLAGRTSIVIAHRLSTIEKADKI
                     LVLHHGRLREMGRHEELLSRGGIYRTLYEMQYRRTAAAPVNRQAAAK"
BASE COUNT          353 a          694 c          710 g          318 t
ORIGIN      
        1 tcggatggtg gagtccccgg cgccctcaat ctcgcaggcg gagagcgtgt cccactcaat
       61 cacggtgatc gcgcccccgt tgacgaagac gtcggtgagg aaagcggggc agcagttggc
      121 ctgcgcgttc aagtgccgga cgcgcagatc cttgccgacc acctccagcg acatcgtctc
      181 cggcccgccg atcacgagcg tgtccgcgcc gaccgtatcc ccttccaggc cgaccagcgt
      241 gaccgcatac tcgcccggct cggcggccgc gaagccgggg aggatcgcct cgaggttgaa
      301 gtagcacatg cacgggcagg ggaagaaaga ggtgtcggcc tcctggaccg tgaccgagcg
      361 gccgtcgatc tcaaacgtcg ccgcataccc cagacagcac tggtagtatg cattgacatg
      421 gtggacgtgg agatccctcc cgaccagctc gacaaacatg tactccgcgg agtcgtcggg
      481 agccacggtc agacaccccg aggaacggac cgcgggattc ggctggccgc cggccgtcga
      541 cgtccaggct tcgacgcagc cgtcgcccat cgccggggtg aacgtggccg ggcactcgag
      601 ggcggggccg ccccggaaca ggtaggacac caaccaggtg aggtcggcca cggtgacgcc
      661 gtagtccccg ttgacgtcgg ccggacggaa atacggctgc tcgggaattc cccggaagag
      721 gtagttgacg gcaccggtga cgtccgcgac gctcacggag ccgtcgccgt tcacgtcccc
      781 gcacacgtcc cctgatgata ccgccgcgag cgggcagacg gccagcgccg cgacccagac
      841 gaaaagtgat gcgcgatgca tatcaacctc gctcaagtag gggtcgaaag agcgttccga
      901 atggtcgaag gagtccgggc atgaagcgag aatattatac gcgccgcccc acatttgagc
      961 aaacactcat ctgccgcggc gtgggcggga cacgataact atctgcccga caggaccttg
     1021 agcatcgatg cgtggagccg cgggttggcc gcgaggatgg accggtcggt gattgcgaac
     1081 ggccggccgt cggtccgggt caccttcccc cccgcctctt cgatgaagag cacggcggcg
     1141 gcggtgtccc acgggtgcag atcgagttcc cagaatccgt cgatccgtcc ggccgccagc
     1201 cagcagaggt cgatcgccgc cgatccgggc cgccgcactc cctgggcgcg cttcaccatg
     1261 cgcgcgaagt agccgaggtt gttgcgccgg gcggtggcca cgttgtagcc gaacccggtc
     1321 gccaaaaggg cgcgctgcag gtcgatctcg cgggccaccc gaaggcgggt gcggttgcag
     1381 tacgccccct gcccgcgcgc cccccggaac atctcgtccc gttcgggatc gtacaccgcg
     1441 ccggcggccg ggagcccccg gaactgcagc gcgatcgaga cgcagtagat cgggaaaccg
     1501 tgggcgtagt tgacggtgcc gtcgagcgga tcgaccaccc actggaactc ggcggcggcg
     1561 cctgtcccgg atccctcttc ggccaggagc gcgtgtcccg ggaacgcgcg gcggaggcgc
     1621 gcggtgatga gacgttcgga tttgacatcc gcctcggtga ccgggtcgat gcggcccttg
     1681 tagcggacgc ggtgggcggc gtggaagcgc ttcttgagca gctccccggc gaggcgcgcg
     1741 gtgtcttcgg caaagcgctg aaaacggctc aggtcgcggg ccgacagttc catggtcctc
     1801 acttggcggc cgcctgccgg ttgaccggcg ccgcggcggt tctccggtac tgcatctcgt
     1861 acaaggtacg atagatcccc ccccggctca agagctcttc atgccgcccc atctcgcgca
     1921 ggcggccgtg gtggagcacc agaatcttgt cggccttctc gatagtcgag agccggtggg
     1981 cgatgacaat cgaggtccgc ccggcgagca gctcctcgag cgcctgctgg atgaggtgct
     2041 ccgtctcggt gtccacggaa ctggtggcct cgtcc
//
LOCUS       DAOOKS010000368         2073 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_67712_length_2073_cov_3.652131, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000368 DAOOKS010000000
VERSION     DAOOKS010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67712_length_2073_cov_3.652131"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /locus_tag="PK112_06420"
     CDS             <1..838
                     /locus_tag="PK112_06420"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPC11718.1"
                     /translation="LAAQRIGLEVSDAARVALRQRDVESVTILYRRTREEMPAFAEEI
                     EAAAQEGIDIQILVTPKKVLAKDGKLIGLECLRNRLGEIDESGRHRPVPIAGSEFRVD
                     LETLIVAISEDSGVDAIGPAKQSGIAITDWNTVKVDKKTLLTTRPGVFAAGDVVRGPN
                     TVVDAIADGKRAARMIDRYVRGLELADAAEPALPRVYVPPVSAELDDLPTVERVETPR
                     APAAWRTRNFAEVEVALSVAEAHCEARRCLRCDLEFTRPPETGQETDHDGAVAVTGGK
                     QL"
     gene            835..1518
                     /locus_tag="PK112_06425"
     CDS             835..1518
                     /locus_tag="PK112_06425"
                     /inference="COORDINATES: protein motif:HMM:NF024900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HPC11719.1"
                     /translation="MTTLAINGLRVQVETGSTILEAAQFLGFPIPTLCHMEGLTPYGA
                     CRLCVVEIGEGPAARLVTSCTYPVQEGLTVRTASERVLRARRMIIELLLASCPQSKVI
                     QDIASAHQVRQQRFRQEHEDCILCGLCVRMCKEQMVAQAIGFYGRGEHRGIGTPFDVK
                     SEVCRLCGGCMYVCPACQLRCTYTDPEKAICGGCANLTPPCVEKEQFHDMMCYMKPCV
                     ACEIPKDAK"
     gene            1539..>2073
                     /locus_tag="PK112_06430"
     CDS             1539..>2073
                     /locus_tag="PK112_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HPC11720.1"
                     /translation="MTLTRVNASAATLTKNRTEDSVVPTSGMCVTCVDGCIGMCEIGK
                     SAYRGHEVIYPQPFGVITTAGEKTYPVDYSHFNIMGTAVGAHGIAADSDKAVFPNVNL
                     EVRFGHDRGIKFRLPWIIPGIGSTDIAKNNWEGLAIGSALAGTGLTIGENVVGMDVEA
                     AIKNGRVVDTVDLKRRVK"
BASE COUNT          359 a          588 c          824 g          302 t
ORIGIN      
        1 gttggcagcg cagcgcatcg gcctggaggt cagcgacgcg gcgcgggtgg ccctccgcca
       61 gcgcgacgtc gagagcgtga ccatcctcta ccggcggacg cgggaggaga tgccggcgtt
      121 cgccgaggag atcgaggcgg ccgcgcagga ggggatcgac atccagattc tcgtgacgcc
      181 gaagaaggtg ctggcgaaag acgggaagct aatcgggctg gagtgtctgc gcaaccggct
      241 gggggagatc gacgagtcgg ggcggcaccg gccggtgccg atcgcgggga gcgagttccg
      301 ggtggatctc gagacgctga tcgtggcgat cagcgaggat tcgggggtcg acgcgatcgg
      361 cccggcgaag cagagcggga tcgcgatcac cgactggaac acggtgaagg tggacaagaa
      421 gacgcttttg acgacgcggc cgggggtgtt cgcggcgggc gacgtggtgc gcggcccgaa
      481 cacggtggtc gacgcgatcg ccgacggcaa gcgggcggca cggatgatcg accggtacgt
      541 gcgcgggctc gagctggcgg atgcggccga gccggctctg ccgcgggtgt acgtgccgcc
      601 ggtgagcgcc gagctcgacg accttccgac ggtggagcgg gtggagacgc cgcgggcgcc
      661 ggcggcctgg cggacgcgca atttcgccga ggtggaggtg gcgctgtcgg tcgccgaggc
      721 ccactgcgag gcgcggcggt gcctgcggtg cgacctcgag ttcacgcggc cgcccgagac
      781 cgggcaggaa acggatcacg acggcgcggt cgccgtcacc ggaggtaagc agctatgacg
      841 acactggcaa tcaacgggct tcgcgtccag gtggagacgg gttcgacaat cctggaggcg
      901 gcgcagtttc tcggttttcc gattccgacg ctgtgccaca tggaggggct cacgccctac
      961 ggggcgtgcc gcctctgcgt ggtggagatc ggcgaggggc cggcggcgcg gctggtgacg
     1021 tcgtgcacgt acccggtgca ggaggggctg acggtgcgga cggcatcgga gcgggtgctg
     1081 cgggcgcggc ggatgatcat cgagctgctc ctcgcctcgt gcccgcagtc gaaggtgatt
     1141 caggacatcg cctcggccca ccaggtgcgg cagcagcgct tccggcagga gcacgaggac
     1201 tgcatcctct gcgggctgtg cgtgcggatg tgcaaggagc agatggtggc ccaggcgatc
     1261 gggttctacg gccggggcga gcaccgcggg atcggcacgc cgttcgacgt gaagtcggag
     1321 gtgtgccggc tgtgcggcgg gtgcatgtac gtgtgcccgg cctgccagtt gcggtgcacg
     1381 tacacagatc cggagaaagc gatctgcggc gggtgcgcga acctcacccc gccgtgcgtg
     1441 gagaaagagc agttccacga tatgatgtgc tacatgaaac cgtgtgtggc gtgtgaaatt
     1501 ccgaaagacg cgaaataaat gaaggaggac aggacatcat gacgctgacc agagtaaacg
     1561 cctcggcggc cacgctgacg aagaaccgga cggaggattc ggtggtcccg acctcgggca
     1621 tgtgcgtcac ctgcgtggac gggtgtatcg ggatgtgcga gatcgggaag tcggcctacc
     1681 gggggcacga ggtgatctac ccgcagccgt tcggggtgat cacgacggcg ggggagaaga
     1741 cctacccggt cgactactcg cacttcaaca tcatgggcac ggcggtgggg gcgcacggga
     1801 tcgcggccga cagcgacaag gcggtgttcc ccaatgtgaa tctcgaggtg cggttcgggc
     1861 acgaccgcgg gatcaagttc cggctcccct ggattatccc ggggatcggc tcgaccgaca
     1921 tcgccaagaa caactgggag gggctggcga tcggctcggc gctggccggg acggggctga
     1981 cgatcggcga gaacgtcgtg ggcatggacg tggaggcggc gatcaagaac ggccgggtcg
     2041 tcgacacggt cgatctgaag cggcgcgtga agc
//
LOCUS       DAOOKS010000369         2072 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_67772_length_2072_cov_3.475954, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000369 DAOOKS010000000
VERSION     DAOOKS010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67772_length_2072_cov_3.475954"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            77..796
                     /locus_tag="PK112_06435"
     CDS             77..796
                     /locus_tag="PK112_06435"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HPC11721.1"
                     /translation="MNNDIELLLKLQVIDYDLGELERSKEYIPDMMANLQREIREAQE
                     KLDAAKQQLSEAKVSQKHFEIEIKAKEGDLARYQQQMMTIKTNKEYDALVAQIDAVKN
                     EIGYREKMMLEAMERIDALEKLLPELTQRFTQVNENNSRQLAVLQEKINSIGDTVTGK
                     QSERVHLLEGIPRRIMSVYERVRKGKGGSAVVPVKKRSCSACHKALTPKLAQEIRRSD
                     TILTCENCGRLLYWDDDISDT"
     gene            841..>2072
                     /gene="guaB"
                     /locus_tag="PK112_06440"
     CDS             841..>2072
                     /gene="guaB"
                     /locus_tag="PK112_06440"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HPC11722.1"
                     /translation="MVKISADYALTFDDVLLVPAHSTVLPKDTDVSTVIGPDLRLNIP
                     LVSAAMDTVTESALAIALARQGGLGIIHKNMSPEEQAGEVDKVKRSESGMIVDPITLP
                     PDRTIGDALEVMSRFRISGIPITEPGGRLVGILTNRDLRFQKNTQQPIGEVMTRENLV
                     TAPEGTDLETARDILHHHRIEKLLIVDDRYMLKGMITVKDIMKKILYPNACKDARGRL
                     RVGAAVGVADLEARADALVAAGVDVLCVDSSHGHSQGVLDAVRQLKARHPNIPLIAGN
                     VATGEGTDALIDAGADCVKVGIGPGSICTTRVVTGAGVPQVTAILNAVAAADRRAIPV
                     IADGGIRYSGDIAKALACGARAVMIGSLFAGTEESPGETVLYEGRSFKVYRGMGSVGA
                     MKRGSSDRYFQEHAEEAS"
BASE COUNT          435 a          740 c          592 g          305 t
ORIGIN      
        1 gcccatccgg cggatgcgct ttttcattat ggagtttccc atgaaccatt ctcagtccgc
       61 caaggagtcc gttcatatga ataacgacat cgagctgctt ctgaagcttc aggtgatcga
      121 ctacgatctc ggcgaactgg agcgctccaa agaatatatc cccgacatga tggccaacct
      181 ccagcgggaa attcgcgaag cacaggagaa gctcgatgcc gccaaacagc agctctcgga
      241 ggccaaggtc tcccaaaaac acttcgaaat cgagatcaag gccaaggaag gggacctcgc
      301 ccggtaccag cagcagatga tgacgatcaa gaccaacaag gagtacgatg ccctcgtggc
      361 ccagatcgac gccgtcaaga acgagatcgg ctaccgcgag aaaatgatgc tggaggcgat
      421 ggagcgcatc gacgccctcg aaaaactcct ccccgaactc acccagcgat tcacccaagt
      481 taacgagaac aactcccgcc agctggcggt tctgcaggaa aagatcaact cgatcggcga
      541 caccgtgacc ggcaagcagt ccgaacgcgt ccatctcctc gagggcatcc cgcgccggat
      601 catgtcggtc tacgagcggg tccgcaaggg caaaggcggc tcggccgtgg tcccggtcaa
      661 gaaacggtcg tgcagcgcct gccacaaggc cctgacgccc aaactggccc aggagatccg
      721 ccgctccgac accatcctca cctgcgagaa ctgcggccgc ctcctgtact gggatgacga
      781 catttcggat acctgaaaca cccggcgcgc gcgccgggag gaaaacgatc acggcctgct
      841 atggtgaaaa tatccgctga ctacgccctg actttcgacg acgtcctgct ggtcccggcc
      901 cactcgaccg tcctgcccaa agacaccgac gtctccaccg tcatcggccc cgacctccgc
      961 ctcaacatcc cgctcgtctc ggccgccatg gacacggtca ccgagtccgc cctcgccatc
     1021 gccctggccc gccagggggg cctcggcatc atccacaaga acatgtcccc cgaggaacag
     1081 gccggcgagg tcgacaaagt caagcgctcc gagtccggca tgatcgtcga ccccatcacc
     1141 ctcccgcccg accggaccat cggcgacgct cttgaagtca tgtcccgctt ccgcatctcc
     1201 ggcatcccca tcaccgagcc gggggggagg ctggtcggca tcctcaccaa ccgcgacctc
     1261 cgcttccaga agaataccca gcagccgatc ggcgaggtga tgacccggga gaacctcgtc
     1321 accgcccccg agggaacgga cctcgaaacc gcccgggaca tcctccacca ccaccgcatc
     1381 gagaaactcc tgattgtcga tgaccgctac atgctcaagg gcatgatcac ggtgaaagac
     1441 atcatgaaga agattctgta cccgaacgcc tgcaaggacg cccgcgggcg cctccgggtc
     1501 ggcgccgccg tcggcgtggc cgacctggaa gcgcgggccg acgccctggt ggccgccggg
     1561 gtcgacgtcc tctgtgtcga ttcctcccac ggccacagcc agggagtgct cgatgcggtg
     1621 aggcagctca aggcgcggca cccgaacatc ccgctcatcg ccggcaacgt ggccaccggc
     1681 gagggcacgg acgctctcat cgacgccggc gccgactgcg tgaaagtcgg catcggcccc
     1741 ggcagtatct gcacgacccg cgtcgtcacc ggcgccggcg tcccccaggt caccgcgatt
     1801 ctcaacgcgg tggccgcggc cgaccgccgc gccatcccgg tgatcgccga cggcggtatc
     1861 cggtacagcg gcgatatcgc caaggctctc gcctgcggcg cccgcgccgt catgatcggc
     1921 tccctcttcg ccggcaccga ggaatccccc ggcgaaaccg tcctctacga ggggcgctcg
     1981 ttcaaggtgt accgcggcat gggatcggtc ggcgccatga aacgcggcag cagcgaccgg
     2041 tacttccagg aacacgccga ggaagcctcc aa
//
LOCUS       DAOOKS010000370         2072 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_67773_length_2072_cov_3.329698, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000370 DAOOKS010000000
VERSION     DAOOKS010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67773_length_2072_cov_3.329698"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            79..>2072
                     /locus_tag="PK112_06445"
     CDS             79..>2072
                     /locus_tag="PK112_06445"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HPC11723.1"
                     /translation="MRVEHEDRVMFSNEDGYYRLRLDPGTHRLRFTHIAYFPAACDVA
                     LSDSTSSRDILLASCLIDVPGTRVYSRAYDPAQEIIVEAIRHKEELLARIRDYRFEGY
                     TKLAVRRLTDDGPAEYFAIIESQLEGWFRQPDRYKEIITARRQSANIPAEVNIITIGE
                     IFNFNANRIDLGQPVVSPTASDALDYYNYYLLDTVLVDGAPAFLLEVEPRTDAHALFA
                     GRILIVDSVYAVAGVDLGLNKGFDSPYLKDLRFTQRMSRFDNDIWMPVEIRFDGDFQS
                     PLPLIPDLRFAYTGLLNRLVINQGIPEERFDRYVLEVAPEADDLDSAAWEQGALLPLT
                     ESEQTGYRYLDSLKHRPLTPRRALGYGVMLAAAGTMAQSAHKFFHFNRVEGPYLGAGA
                     NLTKLVPRSELSLKTGRAFDADCWQHHYRLQVLLHEPTQLKAAIGYRDEIVARPNIYG
                     DQALNPTFANLFFKYDPIDYYLEKGFDLGLSLRILPHTSATLGYRDFRQSTVGNTTEY
                     SLTGIDRAYRSNPVIADGRLRALSAGLAYDSRPLVRFKGREQQVMAYPYTSVRLEFEA
                     ADPDLIATDFHFRRYALSLVRAQSLAGWGFTSLRAYAGGSDYALPPQRFFTVSLENPY
                     EPFLEFKTLSDFNVTGDRVAAFYLEHDFGGTLFRRLGLPLL"
BASE COUNT          345 a          788 c          567 g          372 t
ORIGIN      
        1 cctcgcaccg ccgccgcggc gataatcgag ggaactatct gcgactcggc cacccacgag
       61 cctatcccgc gggtcacaat ccgcgtcgag catgaggacc gggtgatgtt ctccaacgag
      121 gacggctact accgtctccg gctcgacccc ggaacccatc gcctgcgctt cacccacatc
      181 gcctatttcc cggcggcctg cgacgtcgcc ctgagcgact cgacctcgtc ccgggacatc
      241 ctcctcgcct cctgcctgat cgacgtcccc ggcacgcgtg tctactcccg ggcctacgac
      301 cccgcgcagg agattatcgt cgaggcgatc cggcacaaag aggagctgct cgcccggatc
      361 agggactacc gattcgaggg ctacacgaaa ctcgcggtgc gccggctcac tgacgacggg
      421 cctgccgagt atttcgcgat catcgaaagc cagctcgagg gttggttccg gcaacccgac
      481 cgctacaagg agatcatcac cgcccggcgc cagtccgcga acatccccgc cgaagtgaat
      541 atcatcacga tcggcgagat cttcaacttc aacgccaacc gcatcgacct cgggcagccg
      601 gtcgtttccc ccaccgcctc cgacgctctc gactactaca attattacct gctggatacg
      661 gtgctggtcg acggcgcgcc ggcgtttctc ctggaggtcg aaccccgcac cgatgcccac
      721 gctctcttcg ccggccggat tctgatcgtc gattcggtct acgccgtcgc cggcgtcgac
      781 ctcggcctga acaagggctt cgactcgccg tacctgaagg atcttcgctt cacccagcgc
      841 atgtcccgct tcgacaatga catctggatg ccggttgaga tccggttcga cggcgatttc
      901 cagtcgccgc tcccgctcat tcccgacctc cgcttcgcct acaccggcct gctcaaccgc
      961 ctcgtgatca accagggcat ccccgaggag cgcttcgacc gctacgttct cgaggtggcg
     1021 ccggaggccg acgacctcga ctcggccgcc tgggagcagg gcgccctcct gcccctgacc
     1081 gagagtgagc agacgggcta ccggtacctc gactcgctga agcaccgtcc cctcacgccg
     1141 cggcgggccc tggggtacgg cgtcatgctc gcggcggcgg gcaccatggc gcagtccgcc
     1201 cacaagttct tccacttcaa ccgggtcgag ggaccctacc tcggcgcggg cgccaatctg
     1261 accaaactcg ttccccgctc cgaattgtcc ctcaaaaccg gccgcgcctt cgacgccgac
     1321 tgctggcagc accattaccg gctgcaggtg ctgctccacg agccgacgca gttgaaagcg
     1381 gccatcggct accgcgatga aatcgtcgcc cggcccaaca tctacggcga ccaggccctc
     1441 aaccccactt tcgccaacct gttcttcaag tacgacccca tcgactatta ccttgagaaa
     1501 ggttttgacc tcgggctgtc gctgcgcatc ctcccgcaca cctccgccac actcggctac
     1561 cgcgacttcc ggcagtcgac agtcggcaac accaccgaat acagcctgac cggcatcgac
     1621 cgcgcttacc gctccaaccc ggtgatcgcc gacggccgtc tgcgcgccct ctccgccggc
     1681 ctcgcttacg actcccggcc gctggttcgt ttcaagggcc gggagcaaca ggtcatggcg
     1741 tacccctaca cgtcggtgcg gctcgagttc gaagccgccg atccggatct gatcgccacc
     1801 gattttcact tccgccggta cgcgctctcg ctggtgcggg cgcagtcgct ggccggctgg
     1861 ggattcacgt cgctccgcgc gtatgccggc ggctcggact acgccctgcc gccccagcgc
     1921 tttttcacgg tctcgctgga gaacccgtac gagccgttcc tcgagttcaa aacgctcagc
     1981 gatttcaacg tcaccggcga ccgcgtcgcc gcattctacc tcgagcatga tttcggaggc
     2041 acgctcttcc ggcgcctcgg cctgcccctg ct
//
LOCUS       DAOOKS010000371         2068 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_67989_length_2068_cov_2.610532, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000371 DAOOKS010000000
VERSION     DAOOKS010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67989_length_2068_cov_2.610532"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            245..685
                     /locus_tag="PK112_06450"
     CDS             245..685
                     /locus_tag="PK112_06450"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HPC11724.1"
                     /translation="MYKRVLLAAVGLVLMGAVSASAAGERIDWQVIAAGGGNGSSTNY
                     RLSGTVGQTAAGAGSSANYRVNQGFWQDFGSGGCCVLRGDFNEDGAVKVSDLTGLINY
                     LFRGGVAPICLPHGDTNADGAIKVSDLTLLINYLFRGGPAPAAC"
     gene            complement(767..>2068)
                     /locus_tag="PK112_06455"
     CDS             complement(767..>2068)
                     /locus_tag="PK112_06455"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPC11725.1"
                     /translation="EAMLESFTDDYAVLTALSGQEALERIKEHPEIDTIILDIRMARM
                     DGLKAANLIKDIAPEIPIIFHTGFAGNYSEEDIDRDYHPFDFIGKNEPPSRLRRAVKN
                     AVQYHRLGNTGSDLIDAARERYGMVGKSRDMRQIYQRIEQIGPTTSKVMILGPTGSGK
                     ELVARALHARSRRADREMMVVNCSQKDPTMVESELFGHTEGSFTGAVANRVGYFEAAD
                     GSTLFLDEIGELDPATQAKILRAIEYGEVTPVGTCVKRSVDVRVICATHLDLEEQVRS
                     GAFRMDLYYRLKGVTIRLPALRERREDIPELLDYFIERTCRINGVGLKVFEPSARALL
                     VEFDWPGNVRQLSDTVESLIQLSPGYYITYHDVANHLGYESSAAPNGNSGLAEQVWEF
                     KRMKIVQALDRARGNVIAAGEELKIDPANLRKMIKRLNIES"
BASE COUNT          321 a          646 c          701 g          400 t
ORIGIN      
        1 aggggctttt gttgcggggg gcggcccgaa cgcagattcg gcgtcccaaa acccggggcg
       61 atccttctcc cccgcccccg gaatgacagg ttttgcttgc tttttcggcg cgattgcctt
      121 acttgagacg agagcagaaa gcgcccgtcg gccgccgctg ctcggcaggg tcgttcatac
      181 cggatagtcg ccgacgtcaa gacgtacgca agaagatacg cacctcgtca taagggttac
      241 aggcatgtac aaacgcgtcc tcctcgcagc cgtcggcctc gtgctgatgg gggcggtgtc
      301 cgcctcggcc gccggcgagc gaatcgactg gcaggtcatc gccgcgggcg gcggcaacgg
      361 ctcgtcgacg aattaccggc tgagcgggac ggtcggccag acggccgccg gcgccggatc
      421 gtcggccaat taccgggtga accagggttt ctggcaggac ttcgggtcgg gcggctgctg
      481 cgtgctgcgg ggggatttca acgaggacgg ggcggtgaag gtgtcggatc tcaccggtct
      541 gatcaactac ctcttccgcg gcggggtcgc gccgatttgt ctcccgcacg gcgacaccaa
      601 cgccgacggc gccatcaagg tgtcggacct gaccctgctg atcaactacc tcttccgcgg
      661 cgggccggct ccggcggcct gctgaccgcg cggcacaaac acgaagccgg cggcggcctt
      721 gcccgccacc ggctttttca tttcccgccg cgccgtcccc ccacgatcac gactcgatgt
      781 taaggcgctt gatcatcttg cgcaggttgg ccggatcgat cttgagctcc tcaccggccg
      841 cgatcacgtt cccgcgcgcc cggtcgagcg cctgcacgat cttcatccgc ttgaattccc
      901 atacctgttc ggccaggccg gagtttccgt tgggggcggc gctgctctcg tacccgaggt
      961 ggttggccac gtcgtggtag gtgatgtagt aaccgggcga aagttgaatg agcgactcca
     1021 ccgtgtcgga gagctgccgg acattgccgg gccagtcgaa ttcgacgagg agggcgcggg
     1081 ccgacggctc gaacactttc aggccgaccc cgttgatccg gcaggtccgc tcgatgaagt
     1141 agtcgaggag ctcgggaatg tcctcccggc gttcccggag cgcgggcagg cggatcgtca
     1201 cgcctttgag gcggtagtag agatccatcc ggaaagcgcc gctgcgcacc tgttcctcga
     1261 ggtcgaggtg ggtggcgcag atgacgcgga cgtcgacact gcgcttcacg caggtgccca
     1321 ccggggtgac ctcgccatac tcgatagcgc ggaggatttt cgcctgggtg gcgggatcga
     1381 gttcgccgat ctcatcgagg aacagcgtgc tcccgtcggc ggcttcgaaa tacccgacgc
     1441 ggttggccac cgccccggtg aacgacccct cggtgtggcc gaagagttcg gactcgacca
     1501 tggtcgggtc tttctggctg cagttgacga ccatcatctc ccggtcggcg cggcggctcc
     1561 gggcatggag ggcgcgggcg accagctctt tgccgctccc ggtggggccg aggatcatga
     1621 ctttcgacgt ggtcgggccg atctgctcga tccgctggta gatctggcgc atgtcgcgcg
     1681 acttccccac cataccgtag cgctctcgcg ccgcatcgat caagtcgctt cccgtgttgc
     1741 ccagacggtg gtactgcacg gcgttcttga ccgcccgccg gagacgcgag ggtggttcgt
     1801 tcttgccgat gaagtcgaag gggtggtagt cgcggtcgat atcctcctcc gagtaatttc
     1861 ccgcaaaccc ggtgtggaag atgatcggga tttcgggggc gatgtccttg atgaggttgg
     1921 cggctttcag gccgtccatg cgggccatgc ggatgtcgag gatgatggtg tcgatctcgg
     1981 ggtgctcttt gatccgctcg agcgcctcct ggccggagag cgcggtgagg acagcgtaat
     2041 catcggtgaa cgattccagc atggcctc
//
LOCUS       DAOOKS010000372         2063 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_68209_length_2063_cov_3.359562, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000372 DAOOKS010000000
VERSION     DAOOKS010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68209_length_2063_cov_3.359562"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="PK112_06460"
     CDS             complement(<1..777)
                     /locus_tag="PK112_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11726.1"
                     /translation="MKPEPYLDRLIAGGRFRFRRLDIAGIDNEPVFKLNVPEMVEQET
                     CLFHRDVPSGGRYLESLHDTVRTAMAEKAAMPIVRFADGEYAFYRHSLDCNGLYRQAE
                     NAAAIRQAMPAHIEAMRVLARGGRIAPLVFPGNTRKKERGLFSFLRKSKHDDSAIEFL
                     DFLAANDIELTGDNYIPFYAVYAYLVTGRFARLLDGKTVCVVNSDFDENACRSWFRQF
                     SSAPRIAHVPIAAEYVATRWESMREGVLAAVPPGADFCIVG"
     gene            complement(784..1614)
                     /locus_tag="PK112_06465"
     CDS             complement(784..1614)
                     /locus_tag="PK112_06465"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPC11727.1"
                     /translation="MERAPAACILCGSTARSPLIERDGWTVHRCAGCGLGVLDPRPDR
                     EELGRLYEKEYFDSQYREGLRPGSPEMRRRISQEDHRIRFYRRFKKRGLVVDVGCGMG
                     YFLYAAREAGYDVTGVDISDHAASYVRDTLGIPMRTGHIDAVDVPECTVDVATMWHFL
                     EHTDDPRRYIEKVSRWLKTDGLLVIDVPNYEGTDAQKTWLDWVGWQLPYHFHHFTPAT
                     LALLLSQHGFDVIAKKDYHSEVIKERLRNIPGIGLFARLVAKAYSGTSYAVVARKNPT
                     "
BASE COUNT          351 a          680 c          665 g          367 t
ORIGIN      
        1 cccgacgatg cagaagtcgg cccccggcgg gacggccgcg agcacgccct cccgcatcga
       61 ctcccagcgg gtggccacgt actcagcggc aatcggcacg tgggcgatcc ggggtgcgct
      121 cgagaactgc cggaaccagg accggcaggc gttctcgtcg aaatccgagt tgacgacgca
      181 gaccgtcttt ccgtccagga gccgggcaaa gcgcccggtc accaggtagg cgtacacggc
      241 gtagaagggg atgtagttgt cgcccgtcag ctcgatgtcg ttcgctgcga gaaaatctag
      301 gaattcgatc gccgagtcgt cgtgtttcga cttgcgcagg aaggaaaaga ggccccgctc
      361 tttttttcgc gtgttgcccg ggaaaacgag gggtgcgatg cggcccccgc gggcgagcac
      421 ccgcatggcc tcgatgtgcg ccggcatggc ttgccggatc gcggccgcgt tctccgcctg
      481 ccggtacagg ccgttgcagt ccagggagtg ccggtagaag gcgtactccc cgtcggcaaa
      541 ccgcacgatg ggcatggcgg ccttttcggc catggccgtg cgaaccgtgt cgtgcaggct
      601 ctccagatat cggccccccg aggggacatc ccggtggaac aggcaggtct cctgctccac
      661 catctcgggt acgttgagtt tgaacaccgg ttcgttgtcg atgccggcga tgtcgagccg
      721 gcggaatcgg aacctgccgc ccgcaatcag tcggtcaaga tagggttcag gtttcacggg
      781 acctcatgtc ggattttttc ttgccactac ggcatagctc gtccccgaat acgccttcgc
      841 gacgagccgc gcgaacaggc cgatcccggg gatgttgcgg agccgctcct tgattacctc
      901 ggagtggtag tccttcttgg cgatgacgtc gaagccgtgc tgcgaaagca gcagggcgag
      961 agtagccggc gtgaaatgat ggaaatggta cgggagctgc caccccaccc agtccagcca
     1021 ggtcttctgc gcatccgtcc cctcgtagtt cggcacgtcg atgacgagca ggccgtcggt
     1081 cttgagccac cgggagacct tctcgatgta gcgccgcggg tcgtccgtgt gctccaggaa
     1141 gtgccacatg gtggccacgt ccacggtgca ctccggtacg tcgacggcgt cgatgtgccc
     1201 ggtccgcatg gggatcccca gggtgtcccg gacgtaggaa gcggcgtgat cggagatgtc
     1261 cacccccgtc acgtcgtaac ccgcctcccg ggcggcatac aggaaatacc ccatgccgca
     1321 accgacatcg acgacgagcc cccgcttctt gaaacgccga tagaaccgga tccggtggtc
     1381 ctcctgggag atgcgccgcc gcatctccgg cgacccgggc cgcagaccct cgcggtactg
     1441 gctgtcgaag tattcctttt cgtagagccg ccccagctcc tcgcggtcgg gccgggggtc
     1501 caggacgccg agcccgcagc ccgcgcaccg gtgcacggtc cagccgtccc gctcgatcaa
     1561 cggcgaccgg gcggtggatc cgcagaggat gcacgctgcc ggtgcccgtt ccactcaggc
     1621 ccctcccgtc gtgccggctt tttcgatctc ctcgagggta tcgaggatcg cccggtactt
     1681 cacgagcttc tccctcgcag cgccgccgta acagcgagcg cattgacggt gaggagcgat
     1741 ccgttatcga tgtcgcggat agtcagtctg tcgaatcggg atgacccgcc tgccagcagc
     1801 tcctcgagat acgggctctc catgtgtctc atcttcatca gggtgtggat cggtttgtgg
     1861 cggatgcctt acgggcaacg accgcgtaac tgtggcctga ataaccccgg gcgatcatcc
     1921 gcgccaacgg cctcggcgct gccctgtcgg ccgccaacgc gcgcatgtac accgtcgccg
     1981 tcaacaagga cgtcgagctc caggctgagc gcagcaagct gcgcgccagc ttcgaggaca
     2041 gcgtcaagca ggcgcgcctc aac
//
LOCUS       DAOOKS010000373         2063 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_68212_length_2063_cov_3.176793, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000373 DAOOKS010000000
VERSION     DAOOKS010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68212_length_2063_cov_3.176793"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="PK112_06470"
     CDS             <1..286
                     /locus_tag="PK112_06470"
                     /inference="COORDINATES: protein motif:HMM:NF014799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HPC11728.1"
                     /translation="NMHTVKPLDETAIVCAAGETGAVVTAEEHQVGGLGNLVAGVILR
                     RQAETGRPVRFAMVGIADRFGESGQPWQLIKRFGVSAEHIAAKARELVKH"
     gene            301..1473
                     /locus_tag="PK112_06475"
     CDS             301..1473
                     /locus_tag="PK112_06475"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015154933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPC11729.1"
                     /translation="MQYTERVRKLESEGAFVVLAKAKALERQGKSIIHLQIGEPDFDT
                     PANVTEAGIKALRAGQTHYAPSGGVMEAREVCASYLARTRHIPVTAENTIIMPGAKPF
                     LFCALIALINEGDEVIVPNPGYPTYRSIVKFVGAKPVPVRLREENDFRFSIDEFRSLI
                     TPRTRMVILNSPGNPTGGVLTWEDLEAIHAEAKKHDLWILSDEIYSRLVYDGEFKSIA
                     MIPGAMERSIIVDGMSKTYAMTGWRLGFAAMPKELAQYFFTLAINNFSSTCTFAQYAM
                     IEALTGPQDDVDKMVAEFRRRRTVIVDGLNAIDGVRCLNPEGAFYVFPNITGTGLTSQ
                     KFADVMLDEAGVACLSGTAFGEYGEGYVRFSYANSVENIREALARIKRVLAGVRTH"
     gene            1578..>2063
                     /locus_tag="PK112_06480"
     CDS             1578..>2063
                     /locus_tag="PK112_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HPC11730.1"
                     /translation="MTTNILDMRAEPDHRAERVNQLLFADLVSWSAEKRGFAYVRRWD
                     GYRGWADRRFLASLSERRAVAYSRRPAAVVTALQSRLLDFDGRPLPPHLLYYGTIVAL
                     DRLEARRLILVAPDGSRLCVKASSVRPIKEYNRGPLTGRDLVREAGRLVGVPYLWGGI
                     TA"
BASE COUNT          348 a          723 c          618 g          374 t
ORIGIN      
        1 caacatgcat accgttaagc ccttggacga gaccgcgatc gtctgcgccg ccggggaaac
       61 cggcgccgtg gtgacggccg aagaacatca agttgggggc ttgggaaatc tggtcgccgg
      121 tgttatcttg cgccggcagg ccgagaccgg ccgcccggtt cgtttcgcca tggtcggcat
      181 cgccgaccgc ttcggcgagt ccgggcagcc ctggcaactg atcaaacgtt tcggcgtctc
      241 ggccgaacac atcgccgcga aggcacgaga gctcgtgaag cactagagag gagttgcgcc
      301 atgcagtata cggagcgggt tcggaagctc gagtcggagg gcgcgttcgt cgttctggcg
      361 aaagccaaag cgctcgaacg ccagggcaag tcgatcatcc acctccagat cggcgaaccg
      421 gacttcgaca cccccgccaa cgtcaccgag gccggcatca aggccctccg cgccggccag
      481 acccactacg ccccctccgg cggcgtcatg gaggcccgcg aggtctgcgc ttcctacctc
      541 gcccgaaccc ggcacatccc cgtcaccgcc gaaaacacga tcatcatgcc cggtgccaag
      601 cccttcctct tctgcgccct catcgccttg atcaacgagg gcgacgaggt catcgtcccc
      661 aaccccgggt acccgaccta ccgctccatc gtcaaattcg tcggcgccaa gcccgtcccc
      721 gtccgcctgc gcgaggagaa cgacttccgc ttctccatcg atgaattccg ctccctcatc
      781 accccccgca cccggatggt catcctcaat tcgcccggca acccgaccgg cggcgtcctc
      841 acctgggagg atctcgaagc catccacgcc gaggcgaaga agcacgacct gtggatcctc
      901 tccgacgaga tctactcgcg cctggtttac gacggggagt tcaaatcgat cgccatgatc
      961 cccggcgcga tggagcggtc gatcatcgtt gacgggatgt ccaagaccta cgccatgacc
     1021 ggctggcggc tgggatttgc cgcaatgccc aaagaactgg cccagtattt cttcacgctg
     1081 gccattaaca atttctcctc cacctgcact ttcgcccagt acgctatgat tgaagccctc
     1141 accggaccgc aggacgatgt cgacaagatg gtggccgaat tccgccgccg ccgcacggtc
     1201 atcgtcgacg gcctcaacgc gatcgacggt gttcgctgtc tcaaccccga aggcgctttc
     1261 tacgttttcc cgaacatcac cggcaccggc ctgacgtcgc agaaattcgc cgacgtcatg
     1321 ctcgacgagg ccggggtggc ctgtctttcg ggcaccgctt tcggcgagta cggcgagggg
     1381 tacgttcgct tctcctatgc caattcggtc gagaacatcc gcgaggcgct cgcccggatc
     1441 aaacgagtgc tggccggcgt caggacgcac tgaggcggcg cccttgcagt ttcccagccg
     1501 ggccggatgg cccggctttt ttatgttgtc tcccgctgcc gctcgactta taaggttttc
     1561 atatggaatg tgcctgggtg acgaccaaca ttctggacat gcgggccgag cccgaccacc
     1621 gcgccgagcg cgtcaatcag ttgctgttcg ccgacctggt ctcctggtcg gccgagaagc
     1681 gagggtttgc gtacgtgcgg cgctgggacg gttaccgggg atgggccgat cggcgtttcc
     1741 tggcgtcgct gtcggagcgg cgggccgttg cgtacagccg ccgccccgcc gctgtcgtca
     1801 ccgctctcca gtcccgcctg ctcgacttcg acggccggcc gctgcccccg cacctgctgt
     1861 actacggcac catcgtcgcc ctggaccgcc tcgaggcgcg ccggttgatc ctcgttgccc
     1921 ccgacgggag ccgtctgtgc gtcaaggctt cctccgttcg accgataaag gagtacaacc
     1981 ggggcccgct gaccggccgc gacttggtgc gcgaagccgg ccggctggtc ggggtgccgt
     2041 acctctgggg cggcatcacg gcc
//
LOCUS       DAOOKS010000374         2058 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_68458_length_2058_cov_4.026460, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000374 DAOOKS010000000
VERSION     DAOOKS010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68458_length_2058_cov_4.026460"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            457..1011
                     /locus_tag="PK112_06485"
     CDS             457..1011
                     /locus_tag="PK112_06485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPC11731.1"
                     /translation="MTESINILWRRVVDGDRAAWADLVRQFAPLVMTVAVRSGLSAPD
                     AEDCGQQVWLALYRTRKNVRDPARLPAWLIQTTRRRAVRMRQQQSARRQVEADAPEAA
                     SLCLPDDQVVQLEQAAILEGALARLEPRCRDLLRKLFFSPGGTTYAEIARHFGLSPNA
                     LGPLRSRCLARLRKILAELGYPVD"
     gene            1238..1621
                     /locus_tag="PK112_06490"
     CDS             1238..1621
                     /locus_tag="PK112_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11732.1"
                     /translation="MERWINVSLLAPPPSLRTKSSGRVVFDSWRERPAAVRDLPEGLV
                     RRLRLAAPDLDLEIVGEQRRREWRFIARVYRGRTAAGDFVLRAGRQRVVPGGDGFLTW
                     SSSRPPRTVVLISPDREIRFENVSW"
     gene            1615..>2058
                     /locus_tag="PK112_06495"
     CDS             1615..>2058
                     /locus_tag="PK112_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11733.1"
                     /translation="MVNIGSDCERAARRFLQTGRLGGRTPAELAASCDQLVRRETERS
                     ARRAVGLGEQFVRRARAADRTMLPVALRALGWAYVVAGKYQPAAARYLEARRATRDPL
                     ARARIDRILIDVYMYLGRHDEALRRGRRAIAVFRRRKAEADLAKTE"
BASE COUNT          319 a          623 c          778 g          338 t
ORIGIN      
        1 cggcataacc ggccagcggc agaggcacgt ggcgggcgcg caggttgcgg aggaagacaa
       61 tagtgcggcg acagctcagg tcgaagaagg catcgaaggc gcgccgggag aggggcgtgc
      121 gaaggaagtt cgcgataatc gaggccacgg gtgtatctgt catggtcgct gcaccgatcc
      181 tgcattaccg gtccgcatgg cgctgcgagc gggcttgccc tattgacggc aagatacgac
      241 cttacgggtc ggttgacaag ggcaacgcgg ggaaatatgg gtatcaggtg cgagccgtgc
      301 gagcgcggtt tcgtgcaccc ccggaggttt aaatctcacg ccgggcgttc ttggcgttgc
      361 tttttcggcg gcggatcgta taaatacgcg gacgaacgag atccttcacc gacaacggcc
      421 ggtccctgac tgagggctgt ccgcataacc gcagatgtga ccgagagtat caacattctc
      481 tggcggcgag tggtcgacgg cgaccgggcc gcctgggccg acctcgtgcg gcagtttgcc
      541 ccgctagtta tgacggtggc ggtgcgcagc gggctgagtg cgcccgatgc ggaggactgc
      601 gggcagcagg tgtggctggc gctgtaccgg acgcggaaga atgtgcgcga tccggcgcgt
      661 cttccggcct ggctgatcca gacgacccgg cggcgggcgg tgcgaatgcg acagcagcaa
      721 tcagcccgcc gtcaggtgga agcggacgcg ccagaggcgg cgtccttgtg tctgcccgac
      781 gaccaggtgg tacagcttga gcaggcggcg attctcgagg gggcgctagc gcggctcgag
      841 ccgcgctgcc gggacttgct gcggaagctc tttttctcgc cgggcgggac gacctacgcg
      901 gaaatcgccc ggcacttcgg cctctccccc aacgcgctgg gaccgttgcg ctcgcggtgt
      961 ctggcgcgcc tgcgaaaaat ccttgccgaa ctgggctacc cggtggacta agcggggccg
     1021 gtgcggctct tttagggcag agagtgaagg aaaccgccgt atgaaatcat ccgatcgcca
     1081 tcccacgcgc gcgcaaatgc ttgcggccgc ccgctcaggt cccggccgac tccgcggaca
     1141 cctgcagaga tgcgccgagt gcctcaaatg gtttgagatg ctgcggcgct tttctccggc
     1201 gggaagcgcc gggttgatgg cgccatccga ggacgcggtg gagcgatgga tcaacgtttc
     1261 gctgctggcg ccgccgccgt cactccgcac gaagagcagc ggccgggtgg tgttcgactc
     1321 gtggcgggag cggccggccg ccgttcgcga tctcccggag gggctggtgc ggcggttgcg
     1381 cctggcggcg cctgacctgg atctggagat tgtcggcgag cagcggcggc gggagtggcg
     1441 attcatcgcg cgggtgtatc ggggccggac ggcggcgggg gatttcgtgc tccgggcggg
     1501 gcggcagcgc gtggttccgg gcggggacgg tttcctgaca tggtcgtcga gccggccgcc
     1561 ccggacggtt gtgctgatct cacccgaccg ggaaatccgc tttgagaatg tgtcatggtg
     1621 aatatcggaa gtgactgcga gcgcgccgcc cggcgttttc tccagacggg ccggctgggc
     1681 ggcaggacgc cggccgaatt agcggcttcc tgcgaccagc ttgtccgtcg ggaaacggag
     1741 cgatcagccc ggcgcgcggt gggtctggga gagcagtttg tccgccgggc ccgcgccgcc
     1801 gaccgcacga tgctcccggt tgcgcttcgc gcactcggct gggcgtatgt cgtggcgggc
     1861 aagtaccagc cggcggcggc gcgctatcta gaggcgcggc gggccacgcg cgacccgctc
     1921 gcccgcgcgc gcatcgaccg gatactgatc gacgtgtaca tgtacctggg ccggcacgac
     1981 gaggcgctgc ggcgcggccg ccgggcgatc gcggtgttcc gcaggcgcaa ggccgaggcc
     2041 gatctggcca agacggaa
//
LOCUS       DAOOKS010000375         2053 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_68699_length_2053_cov_3.980480, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000375 DAOOKS010000000
VERSION     DAOOKS010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68699_length_2053_cov_3.980480"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1905)
                     /locus_tag="PK112_06500"
     CDS             complement(<1..1905)
                     /locus_tag="PK112_06500"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HPC11734.1"
                     /translation="MKKKTRTPSQLALLILVCFFLSGFTGLIYEVLWTRMIVKIIGGA
                     PFAISIILTIFMGGLGLGSYLAGRVVDRVKQPEELVRLYGYLELMVGAYGLVLPGLMA
                     LFRPLFAAMYNGVFEYGMIYNLLTFAGCALLLILPVTFMGATLPVLSRFYVSRLGHLG
                     THTGRLYGLNTIGAGFGSLATGFWLIAWFGMWGTLATAVALNAAIGLTCVLVGGRARI
                     TRGRPAEAGATGPAEVAETPPRAVISGALVIFAVSGFCSMSYEVIWTKLLGLIVGPTT
                     YSFTVVLVTFIIGLALGAMLFGWLADRTRKPAPLLLVTQVAAAAAALGVSQVLGNSQL
                     FFTKLIATFQDNFTALNAAKAALLFLFMLPPTLCLGATFPLVGKIFTPSTARVGRSIG
                     FAYAVNTIGAVLGSFLAGFVIVPALGKANGLSLVIAIQLAGALVFGWVLLRAGAVRRR
                     IWLPAAGAAAAALVLVFAYPRWDHRLLALGKYHRIADQGVKADQVGWWSALLRGPEIL
                     APLFNADVVYYGDGVAGTTTVLKTRDAMGNEDLSMTNAGKPEASTRGDMPTQTLLAHF
                     PLLFGNEAKQVMVVGLASGVTAGEILRYPIERLDIVEISREVVTASEYFRPWNHGVLD
                     DPRARLIVQDG"
BASE COUNT          376 a          761 c          625 g          291 t
ORIGIN      
        1 gccgtcctgc acaatgagcc gggcccgcgg gtcatcgagc acgccgtggt tccacggccg
       61 gaaatactcg ctcgccgtca ccacttcccg gctgatctcg acgatgtcga gccgctcgat
      121 cgggtagcgg agaatctccc ccgcggtcac accgctcgcg agacccacga ccatcacctg
      181 cttcgcctcg ttgccgaaca gcagggggaa atgggccagg agcgtctggg tgggcatgtc
      241 gccgcgggtg gaagcctcgg gcttgcccgc gttggtcatg gacaaatcct cgttccccat
      301 ggcgtcgcgc gtcttgagca cggtggtggt cccggccacc ccgtccccgt agtacacgac
      361 gtcggcattg aacagcggcg cgaggatctc cggtccgcgc agcagcgccg accaccaccc
      421 cacctggtcg gccttcactc cctggtccgc gatccggtgg tacttgccca gggccaggag
      481 ccggtggtcc cagcgcgggt aggcgaacac gaggaccagc gcggccgccg ccgcgcccgc
      541 cgccggcagc cagatgcgtc ggcgcaccgc ccccgcccgc aggagcaccc atccgaaaac
      601 cagcgccccg gcaagctgaa tcgcgatcac caggctcagc ccattcgctt tccccagtgc
      661 cggcacgatc acgaaccccg cgaggaacga cccgaggacc gctccgatgg tgttcaccgc
      721 gtaggcaaac ccgatcgacc gccccacccg cgccgttgag ggcgtgaaga ttttcccgac
      781 cagggggaag gtggcgccga gacagagggt cggcggcagc atgaacagga aaagcagcgc
      841 cgccttggcc gcgttgagcg ccgtgaaatt gtcctggaac gtcgcaatca gcttggtgaa
      901 gaacaactgg ctgttcccca gcacctggct cacgcccagg gccgcggccg ccgccgccac
      961 ctgcgtcacc agaagcagcg gggccggttt ccgcgtccga tcggccagcc acccgaacag
     1021 catcgctccc agcgccagcc cgataatgaa cgtcaccagc accactgtga acgagtaggt
     1081 ggtcggcccc acaatcagcc cgaggagttt tgtccagatc acctcgtacg acatcgagca
     1141 gaagccggac acggcgaaaa tcacgagcgc gccgctgatg actgcccgcg gcggcgtctc
     1201 cgcgacttcc gccgggcccg tcgctcccgc ctccgccggc cggccccggg tgatccgggc
     1261 gcgccccccg accagcacac aggtcagccc gatcgccgca ttcagcgcca ctgccgtcgc
     1321 cagcgttccc cacatcccga accacgcgat cagccagaac ccggtggcga gcgaaccgaa
     1381 tcccgccccg atcgtattca aaccgtacag ccgccccgtg tgcgtcccca gatgccccag
     1441 gcgggagaca tagaaccggc tcaacaccgg cagcgtcgct cccatgaagg tcaccgggag
     1501 aatgagcagg agagcgcagc ccgcgaacgt gagcaggttg tagatcatcc cgtattcgaa
     1561 cacgccgttg tacatggccg cgaacagcgg tcgaaagagc gccatcagcc ccggcagcac
     1621 cagcccgtac gcccccacca tcaactccag gtatccgtac agccgcacga gttcttccgg
     1681 ctgcttcacc cggtcgacca cccggcccgc caggtagctc cccaagccga gcccgcccat
     1741 gaaaatcgtg aggatgatgg agatcgcgaa gggtgctccg ccgatgatct tcacgatcat
     1801 ccgcgtccac agcacctcgt agatgagccc ggtgaatccg gagagaaaga aacagaccag
     1861 gatcaggagg gcgagctgcg atggtgtgcg cgttttcttc ttcactgcgg tcaccgttgg
     1921 tcgtctgccg gcgcggtctt gcggaatata cgctctccgc tgcccgcgcg catggcaatt
     1981 tcagcgtccc gcccgtgccg gcggccgact gcgctgccgt cggtcccccg gggcggcgcc
     2041 ccgcggccgc ccg
//
LOCUS       DAOOKS010000376         2053 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_68706_length_2053_cov_3.287788, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000376 DAOOKS010000000
VERSION     DAOOKS010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68706_length_2053_cov_3.287788"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="PK112_06505"
     CDS             <1..708
                     /locus_tag="PK112_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11735.1"
                     /translation="VPDPASGRAVKGQPRDLVVNSRGEIILIDTHSECLDVLDFRGAL
                     LERLRLDLLLGDTALSLRPQAVALGPGDRTIVATAGDVAGVLILDQTFALERTIGVDD
                     PAQSPFRTIMALTVDDSTLYVTDLYAEPAVKLFDLSGRYLRGFAGHDVDRADLSFPSG
                     IAVMTDTAGEKTVWVTDGLRQVVKVYRPNGAFLAFVGGFGLKPGEFRYPGDIAFNGTA
                     FYVLERAGGRVQRFVID"
     gene            1150..>2053
                     /locus_tag="PK112_06510"
     CDS             1150..>2053
                     /locus_tag="PK112_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11736.1"
                     /translation="MKTVFLLGVAAVALLAGGALAGDYHTGTTLNCAECHVMHYSQTH
                     GYNADGSGITVGLGADGPYHFLLRNEVNTLCLSCHDNQTFAPDVLAENGGNVTANGRQ
                     AGALNRDNVAPYYDAAGHTLGSTDVAPGGTFSNDHGLNCIDCHTQHGRTSGGQSIYRN
                     ANIFINGTPATPYMTYAIGTNDLTKDIFELSATYGGNHYDMQNVQFNEPNVTASGYGA
                     FCQQCHTDFHGSSSSANMRNTAAPTQTEWLRHPTADANIGALTGGHSSLSKFTGQAYR
                     VQVMSATGNWGTWGAAWTGAPTTLT"
BASE COUNT          404 a          682 c          573 g          394 t
ORIGIN      
        1 gtccccgacc ccgcgagcgg gcgggccgta aaggggcagc cgcgggacct ggtggtcaac
       61 agccgcggcg agatcatcct catcgacacc cacagcgagt gtctcgacgt gctcgatttt
      121 cgcggcgccc tgctcgaacg cctccgcctc gacctcctgc tgggcgatac ggcgctctcc
      181 ctccgcccgc aggcggttgc cctcggaccc ggcgaccgca ccatcgtggc gaccgccggc
      241 gacgtcgcgg gggtgctcat ccttgatcag actttcgccc tggagcggac tatcggcgtc
      301 gatgaccccg cccagtcccc gttccgcacc atcatggccc tcaccgtcga tgacagcacc
      361 ctctacgtaa ccgacctcta cgccgagccg gccgtgaaac tcttcgatct ctccggccgg
      421 tacctccgcg gttttgccgg ccacgatgtc gaccgggccg acctgtcctt cccctctggc
      481 atcgctgtca tgaccgacac ggcgggggag aagactgttt gggtgaccga cggtctgcgg
      541 caggtggtca aggtgtaccg tccgaacgga gcgtttctgg cgttcgtcgg cggtttcggc
      601 ctgaagccgg gggagtttcg ctaccctggg gatattgcct tcaatggtac cgcgttttac
      661 gttctggagc gagccggtgg tcgcgtccaa cgctttgtga tcgactaaga taacccagaa
      721 tatcgggatt ctccggatct aatccctgcc aatatgtaca catatgccgg gacgaatctg
      781 tactttcaag tagatgttag ggaatggctt aacgtctcat ttcggagcaa tgatgatatt
      841 ttgtgtcatt taagtgtcat ttagttcttg acaaatagga gctttattga tatcctttgc
      901 ccagttatga acgcagatca ccggatgaac caacttaggt ctacgcactg agaagttccc
      961 tgatcgagaa acttggaaac gagaatggcc aaccaccgga atgattcgac catcgcttgt
     1021 gacaggaatc acaaatatag atcacgcagg tctccaaact tcggattcgc ccaatcactc
     1081 acaatgcacc catactatca ccattccacc ccgtggaagt atgctttcac gaaactggag
     1141 gaaagcagca tgaagactgt cttcctgctc ggagttgccg cggtggcgct tctggccggc
     1201 ggtgcgctgg ccggcgacta ccacaccggc acgaccctca actgcgccga gtgccacgtg
     1261 atgcactaca gccagaccca cggctacaat gccgacggtt ccggcatcac ggtcgggctt
     1321 ggggccgatg gtccttatca tttcctgctg cgcaacgagg tcaacacgct ctgcctctcg
     1381 tgccatgaca accagacgtt tgctccggat gtcctggcgg agaacggcgg caacgtgacg
     1441 gccaacggtc gccaggcggg cgccctgaac cgcgacaacg tcgctcccta ctacgacgct
     1501 gccggccaca cgctcggctc gaccgacgtg gctccgggcg gcacgttcag caatgaccac
     1561 ggtctgaatt gcatcgactg ccacacgcag cacggtcgca cctccggcgg tcagagcatc
     1621 taccggaacg ccaacatctt catcaacggc actccggcga ccccgtacat gacctacgcc
     1681 atcgggacca acgatctgac gaaggacatc ttcgagctgt ccgcgaccta cggcggcaat
     1741 cactatgata tgcagaatgt tcagttcaac gagccgaacg tgaccgcgtc cggctacggc
     1801 gcgttctgcc agcagtgcca caccgacttc cacggttcgt cgagcagcgc caacatgcgc
     1861 aacacggccg ctccgacgca gaccgagtgg ctgcgccacc ccaccgcgga tgccaacatc
     1921 ggcgctctca ccggcgggca ctccagcctc agcaagttca ccggccaggc ctaccgcgtg
     1981 caggtcatgt cggcgaccgg caactggggc acctggggcg cggcctggac cggcgctccg
     2041 actacgctca ccc
//
LOCUS       DAOOKS010000377         2052 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_68768_length_2052_cov_3.237356, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000377 DAOOKS010000000
VERSION     DAOOKS010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68768_length_2052_cov_3.237356"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /gene="manB"
                     /locus_tag="PK112_06515"
     CDS             <1..470
                     /gene="manB"
                     /locus_tag="PK112_06515"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="converts mannose-6-phosphate to
                     mannose-1-phosphate; the resulting product is then
                     converted to GDP-mannose by ManC which is then used in the
                     synthesis of mannose-containing glycoconjugates that are
                     important for mediating entry into host cells; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HPC11737.1"
                     /translation="DQLNGDLAYRIGYALASVLKPRSIAVGRDTRVSSDLLFEHLARG
                     INDYGADVIDLGLISTDALYFAVGKFGYDGGVMVTASHNPKEYNGFKVCRKEAVPLSG
                     QEGLNQVLRFIQDGEEMPRSPSPGVVARKDILSEYADHCLSFIDISRVRSSTS"
     gene            467..1291
                     /locus_tag="PK112_06520"
     CDS             467..1291
                     /locus_tag="PK112_06520"
                     /inference="COORDINATES: protein motif:HMM:NF014879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11738.1"
                     /translation="MIDAGNGIAGRTVPPVLDRLPIRTTRLYFELDGTFPNHPASPIE
                     LENLADLIRAIAETGADFGAAFDGDADRMFLVDRKGRQIGGDMVTAMVAKSLLKRHPG
                     ETILYNLICSRAVPELVERMGGTAIRTRVGHALIKPLMKKHNAIFGGEHSGHFYFRDN
                     WFADSGLIAFLVCLELLSEERRPLDEVVRDLDPYVRSGEINSRVADQKAKIEAIAEAY
                     GDGRQDRIDGLTVAYDTFWFNLRPSNTEPLLRLNIEADSPRILEVQKAKLLGLIRS"
     gene            1294..>2052
                     /locus_tag="PK112_06525"
     CDS             1294..>2052
                     /locus_tag="PK112_06525"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPC11739.1"
                     /translation="MVDTPIRLLVVDDDPLLRELLQDTLDTLGYRVTAAADGIEALER
                     LAQGSFDLVITDIKMPGMDGIALLKRVNRLYPDLPVLFITGVASPEVIGQASPVGFLA
                     KPFRISHIERLISDALHWRKERVEHPIRRVMVVDDDDLFREMLADTLTGHGFTACAVQ
                     NGPQALSELENGPVDAVITDIKMPGMDGIALMQAIKERHPELPVVLITAFMSEAELGP
                     ATPAARADGFLRKPFEIDRILELLDSLAPSPVDGR"
BASE COUNT          350 a          655 c          683 g          364 t
ORIGIN      
        1 ccgaccagct caacggagac ctggcctacc gcatcggtta tgcgctggcc agcgtgctga
       61 aaccgagatc catcgcggtc ggtcgcgata cgcgcgtatc ctccgacctg ctctttgagc
      121 acctcgcccg ggggataaac gactatggcg ccgacgtgat cgacctcggt ctcatttcca
      181 ccgacgccct ctattttgcc gtcgggaaat tcggttacga cggcggggtg atggtgaccg
      241 ccagccacaa cccgaaagaa tacaacgggt tcaaggtctg ccgcaaagag gctgtgccgc
      301 tctcgggcca ggagggcctc aaccaggtgc tcaggtttat ccaggacggc gaggagatgc
      361 cccgctcccc ctctccgggg gtggtggcgc gcaaggatat cctctccgaa tacgccgacc
      421 attgcctttc gttcatcgac atttcccgcg tccggagttc cacgtcgtga tcgatgctgg
      481 caacgggatc gccgggcgga ccgtgccgcc ggtcctcgac cgccttccga tccgcacgac
      541 gcggctctat ttcgagctgg acgggacttt cccaaaccac cccgcctcgc cgattgagtt
      601 ggagaacctg gccgacctga tccgggcgat tgcggagacg ggggcggatt tcggggccgc
      661 gttcgacggc gatgcggacc gcatgttcct cgtggaccgc aagggccgtc agatcggcgg
      721 ggacatggtg acggcgatgg tggccaagtc gctgctgaag agacatccgg gggagacgat
      781 cctctacaac ctgatctgtt cgcgggcggt tcccgaactg gtggagcgga tgggggggac
      841 ggcgatccgg acgcgggtcg gccacgcgct catcaagccg ctcatgaaga agcacaacgc
      901 gattttcggg ggcgaacact ccgggcattt ctatttccgc gacaactggt tcgccgactc
      961 cggcctcatc gcgttccttg tctgcctcga gctgctctcc gaggagcggc gaccgctgga
     1021 cgaggtggtg cgcgacctcg atccgtatgt ccgctcgggc gagatcaaca gccgggtggc
     1081 cgaccagaag gcgaaaatcg aggcgatcgc cgaggcctac ggcgacggcc ggcaggaccg
     1141 gatcgacggc ctcacggtgg cctacgacac cttctggttc aatctccggc cgtccaacac
     1201 cgagcccctg ctgcggctga atatcgaggc cgacagcccc aggatcctcg aggtgcagaa
     1261 agcgaaactg ctgggcctga ttcgctcctg agtatggtgg acacgccgat ccggttacta
     1321 gtggtcgatg acgacccgct cctgcgcgag ctgctgcagg acacgctcga cacgctcggg
     1381 taccgggtga cggcggcggc cgacgggatc gaggcgctcg agcggctcgc ccaggggtcg
     1441 ttcgatctcg tcatcaccga catcaagatg ccgggcatgg acgggatcgc gcttttgaag
     1501 cgggtcaatc gcctctaccc cgatctcccg gtgctgttca tcaccggggt cgcctctccg
     1561 gaggtgatcg ggcaggcgtc gccggtcggt tttctggcca agccgttccg aatctcgcac
     1621 atcgagaggc tcatttcgga cgcgctgcac tggcggaagg agcgggtgga gcatccgatc
     1681 cgccgggtga tggtggtgga cgacgacgat ctgtttcggg agatgctcgc cgacacgctc
     1741 accggccacg ggttcacggc gtgcgcggtg cagaacggtc cgcaggcgct gtccgagctg
     1801 gagaacggtc cggttgacgc ggtgatcacc gacatcaaga tgcccggcat ggatgggatc
     1861 gcgctcatgc aggcgatcaa ggagcggcac ccggagctcc cggtggtgct gatcacggcg
     1921 tttatgagcg aggcggagct cgggccggcg acgcccgccg cccgggccga cgggtttctc
     1981 cgcaagccgt tcgagatcga tcgcatcctc gaacttctgg attccctcgc accctcgccg
     2041 gtcgacggcc gg
//
LOCUS       DAOOKS010000378         2045 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_69135_length_2045_cov_3.032161, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000378 DAOOKS010000000
VERSION     DAOOKS010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69135_length_2045_cov_3.032161"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2035)
                     /locus_tag="PK112_06530"
     CDS             complement(<1..2035)
                     /locus_tag="PK112_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11740.1"
                     /translation="MKGRVVLPFHGSYYDFAHVLRHELVHVFQLSKMEAVLARMGRVN
                     FGRPPLWFTEGLAEYWSKAWDAEAEMVVKDMAVNELQLGIEDLYRVAGSYYMYKLGES
                     ICTFIDSTYGPEKLTLLFENLAKGKTFDEVVKLTLGDDLREVSRKWRYSLKKCYYPQL
                     EEAGLPDMESVQITRDGYSVRGVPIMWDAGDGRREWIVFKANRMGYSGIYMTPSGEGR
                     GRRVKTLLKGERSSDFESLYLLRSGIDATDSGMVVFSSKSKGNDVIYVYNLREERVTH
                     RYEFADLAGAHSPRLSPDGTQLVFAGVTLGGMADVYALNLADGAYRPILEDIYYDTDP
                     VFSPDGNQIVFASDRGPEGKEGALNLFSYALGSGTVRQLTFGRYRDQTPDGAAGGIYF
                     TSDRNGTFNIYRLDSLGGLTRQSTYVTGAFEPRVTPDGKGLVYSGYQKMGYQVFRMEL
                     PAEPAPVAQQTPPPTPSWRPDRIDSKYVKSSVKYETDYSLDIAQSAVGYDPVYGSLGG
                     FQFAMSDMLGNHSYHFLLTNTANTKDEFLQSFNIGVTYLNRERRLNWGIGAFHLYDEY
                     FNDYDGFYWERQAGMIGVFSYPFSKFHRLDVTTVGRHSNREEGTRTRDRRAVLMTNYL
                     SWVYDNSLWDISGPIDGRRYNLTVGLTTRLDGVRSFNRLAAADVRHYFRLGRVS"
BASE COUNT          381 a          718 c          585 g          361 t
ORIGIN      
        1 ccgagacccg cccgagccgg aaataatgcc ggacgtcggc cgccgccagc cgattgaacg
       61 accgcacccc gtccagccgc gtggtcagcc cgacagtgag attgtaccgc cgcccgtcga
      121 tcggccccga aatgtcccac agcgagttgt cgtacaccca actgagatag ttcgtcatca
      181 acaccgcccg gcggtcccgc gtccgtgtcc cctcctcccg gttcgaatgc cgccccaccg
      241 tcgtcacgtc cagccggtgg aacttggaga acgggtagct gaacaccccg atcatccccg
      301 cctgccgctc ccagtagaaa ccgtcgtagt cgttgaaata ctcgtcgtac aggtggaacg
      361 ccccgatccc ccagttgagc cgccgctccc ggttgaggta ggtcactccg atattgaacg
      421 actgcagaaa ctcgtccttg gtattggccg tgttggtcag gaggaaatgg tacgagtggt
      481 ttcccagcat gtccgacatg gcgaactgga aacctccgag cgacccgtag accgggtcgt
      541 agccgaccgc cgactgggca atatcgagcg agtagtcggt ctcgtacttc accgagctct
      601 tcacgtattt cgagtcgatc cgatcgggcc gccaggacgg tgtcggcggc ggcgtctgct
      661 gcgctaccgg cgccggctcg gccgggagct ccatgcggaa cacctggtac cccatcttct
      721 ggtacccgct gtacaccaac ccctttccat ccggcgtcac ccgcggttca aacgcccccg
      781 tcacgtaggt cgactgccgc gtcaggccgc cgaggctgtc gagccggtaa atgttgaatg
      841 tcccgttccg gtccgaggtg aaatagatcc cgccggccgc gccgtccggc gtctggtcgc
      901 ggtaacgccc gaacgtcaac tgccgcacgg tccccgatcc gagggcgtag gagaacaggt
      961 tgagcgcccc ctctttcccc tccggcccgc ggtcggaggc aaacacgatc tggttcccgt
     1021 cgggcgaaaa caccggatcg gtgtcgtagt agatgtcctc cagaatcggg cggtaggcgc
     1081 cgtccgccag attgagcgcg tagacgtcgg ccatcccgcc gagcgtcacc cccgcgaaca
     1141 ccaactgcgt gccgtcgggc gaaagccggg gggaatgcgc tcccgcgaga tcggcgaact
     1201 cgtagcggtg cgtgacccgc tcctcgcgca gattgtagac gtagataacg tcgttccctt
     1261 tcgacttcga tgagaacacg accatccccg agtcggtcgc gtcaatcccc gaccgcagaa
     1321 ggtagaggga ttcgaaatcg gacgagcgct cccctttcag gagcgtcttt acccgccggc
     1381 cgcgcccctc accggagggg gtcatgtaga tccccgagta gcccatccga ttggccttga
     1441 acacgatcca ctcccgccgc ccgtcgccgg cgtcccacat gatcggcacg ccccgcaccg
     1501 aatagccgtc gcgcgtgatc tgcaccgact ccatatccgg cagccccgcc tcctccaact
     1561 gcgggtagta gcacttcttg agcgaatacc gccacttccg ggagacctcg cgaaggtcgt
     1621 caccgagcgt cagcttcacc acctcgtcga acgtcttccc cttggcgaga ttctcaaaca
     1681 gcagagtgag cttctccggt ccgtaagtcg aatcgatgaa agtgcagatg gactcgccga
     1741 gcttgtacat gtagtaagag cccgccaccc ggtagaggtc ctcgattccg agctggagtt
     1801 cgttgacggc catgtccttg accaccatct cggcctcggc gtcccacgcc ttcgaccagt
     1861 actccgccag tccctccgta aaccacagcg gcggccgccc gaaattcacc cgccccatgc
     1921 gcgcgagcac cgcctccatc ttggagagct ggaacacgtg aaccagttcg tggcggagga
     1981 cgtgcgcgaa atcgtagtac gacccgtgga acggcagcac cacccgcccc ttcatgaact
     2041 cggtg
//
LOCUS       DAOOKS010000379         2044 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_69168_length_2044_cov_3.607843, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000379 DAOOKS010000000
VERSION     DAOOKS010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69168_length_2044_cov_3.607843"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1108
                     /locus_tag="PK112_06535"
     CDS             <1..1108
                     /locus_tag="PK112_06535"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HPC11741.1"
                     /translation="LAPGDYLIFVSGQTYTGFPCSMGPWEYALTATSVPAEPTPCAAS
                     GGCDEYISQVQIGTIDNSSDCTEYGDYTAISTDVEVGGSYAITVTNGSGYSSDQCGVW
                     IDWNDDVVFDASEMVTLDVSTGVGPYTGTVVVPEGAVEGGVTMRVRIMYTGTLAPCGT
                     TSYGEVEDYTLNVGGAAPTFMVAPDPILVLEKFALNPTWGDVYIGDGALPDGAAAAME
                     NITVALGGCPVTVGVTQVIPGGVGEIPGDVMDVKFSLKEYVLCEEARQGGLIWDDIDS
                     FFDVFYEIDGVPGTLNLQVVVRGHRSGDLNLDGNVNVTDLTMFVSYLFRGGDAPLEPA
                     VANVDASPNQTPNVADLTYMIGYMFRGGPMLKHQ"
     gene            complement(1147..>2044)
                     /gene="mutM"
                     /locus_tag="PK112_06540"
     CDS             complement(1147..>2044)
                     /gene="mutM"
                     /locus_tag="PK112_06540"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HPC11742.1"
                     /translation="TMPELPEVEVVCRGLRRVVVGRTIGRVTVRAPKSSVTVSPSLGA
                     RFLDEALRGRRIESVDRRGKNILIRLAGRRTLWVHLKMTGRFLWLPRTEPIAAHDLVI
                     VDFLPEAGADDGHHLRFNDYRRFGRWRLYPDDELDLQEGLRALGPEPSALSADDFAAL
                     CRRRPRRIKAALLDQSFLAGIGNIYADEALYAARLHPLRLTTAMSRRKLRELHTHIVR
                     LLRRAIHLMGTSVDSYSGVNGRPGRFQSRLQVYGREGKPCGRCGAAIVRRKIGARSAH
                     FCPRCQRAGEAAPGLPRKNPPA"
BASE COUNT          318 a          633 c          754 g          339 t
ORIGIN      
        1 gctcgccccc ggcgattacc tcatcttcgt ctccggccag acgtacaccg gcttcccgtg
       61 ctcgatgggt ccgtgggagt acgcgctgac ggcgacctcc gtgccggccg agccgactcc
      121 gtgcgcggcc agcggcggat gcgatgagta catctcgcag gtgcagatcg ggacgatcga
      181 caattcgtcc gattgcaccg agtacggcga ctacaccgcc atctcgaccg acgtcgaagt
      241 gggcggcagc tacgcgatca ccgtcaccaa cggcagcggg tactcgtccg accagtgcgg
      301 cgtctggatc gactggaacg acgacgtggt ctttgacgcc tccgagatgg tcactctgga
      361 tgtttccacc ggcgtcggtc cgtacaccgg cacggtggtg gttccggaag gggccgttga
      421 gggcggtgtg acgatgcgtg tccgcatcat gtacaccggg acgctcgcgc cgtgtgggac
      481 gacgagctat ggcgaggtcg aggactacac gctgaacgtc ggcggcgctg cgccgacatt
      541 catggtggcg ccagacccga ttctcgtgct cgagaaattc gcgttgaacc cgacctgggg
      601 cgacgtgtac atcggcgacg gtgcgcttcc ggacggcgcc gcggccgcca tggagaacat
      661 caccgtggcg ctcggcggct gcccggtcac ggttggcgtg acgcaggtta ttccgggcgg
      721 tgtcggcgaa atcccgggcg acgtcatgga cgtgaagttc tcgctgaagg agtatgtcct
      781 ctgcgaggag gcgcgccagg gcggcctgat ctgggacgac atcgactcgt tctttgacgt
      841 gttctacgag atcgacgggg tgccgggaac gttgaacctg caggttgttg tgcggggaca
      901 ccggtcgggc gacctgaatc tcgacggcaa cgtcaatgtg actgacctga cgatgttcgt
      961 ttcgtacctg ttccgcggcg gcgacgctcc gctggagccg gccgtggcga acgtcgatgc
     1021 ttcaccgaat cagaccccga acgtggctga tctgacctac atgatcggct acatgttccg
     1081 cggcggtccg atgttgaagc accagtaaga cttgtcgcgt aaagcgcgag tcacaacccg
     1141 ccggcgtcag gccggcgggt tttttcttgg cagccccggg gcggcctcac ccgcccgctg
     1201 gcaccgcggg cagaagtggg ccgaccgggc gccgatcttc cgccgcacaa tcgccgcccc
     1261 gcaccgcccg cacggtttcc cctcgcgccc gtagacctgg aggcggctct ggaagcggcc
     1321 gggccggccg ttcaccccgg agtagctgtc caccgaggta cccatgaggt ggatggcgcg
     1381 gcgcaggagc cgcacgatgt gggtgtgaag ctcgcgcagt ttgcgccgcg acatggcggt
     1441 ggtcaggcgg agcgggtgga ggcgggcggc gtagagcgcc tcgtcggcgt agatgttgcc
     1501 gatcccggcg aggaacgact ggtcgaggag ggcggctttg atccggcggg ggcggcggcg
     1561 gcagagggcg gcgaagtcgt ccgccgacag cgccgacggc tcggggccga gcgcgcgcag
     1621 tccctcctgg aggtcgagct cgtcgtcggg gtacagccgc caccggccga accggcggta
     1681 gtcgttgaag cgcaggtggt gtccgtcgtc ggcgccggct tcggggagga agtcgacgat
     1741 aacgaggtcg tgcgcggcga tcggttcggt ccggggcagc cagaggaagc ggccggtcat
     1801 tttcaggtgg acccagaggg tgcggcggcc ggcgaggcgg atgaggatgt tcttgccgcg
     1861 ccggtcgacg gactcgatcc gccggcctct cagggcctcg tccagaaacc gcgcgccgag
     1921 cgaggggctg acggtgacgg aggatttagg cgcgcggacg gtcacccggc cgatggtgcg
     1981 gccgaccacg acgcggcgca ggccgcggca gacgacttca acctcgggga gttcgggcat
     2041 ggtt
//
LOCUS       DAOOKS010000380         2041 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_69329_length_2041_cov_3.070997, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000380 DAOOKS010000000
VERSION     DAOOKS010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69329_length_2041_cov_3.070997"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="PK112_06545"
     CDS             complement(<1..210)
                     /locus_tag="PK112_06545"
                     /inference="COORDINATES: protein motif:HMM:NF014534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HPC11743.1"
                     /translation="MLKKVTARHFDLTPEIRAKAEAEMDGLTRFFDNIVSAEMVLDAE
                     RHRRVAELKVNVFNNLIQGRGETDDM"
     gene            complement(469..1893)
                     /gene="rpoN"
                     /locus_tag="PK112_06550"
     CDS             complement(469..1893)
                     /gene="rpoN"
                     /locus_tag="PK112_06550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HPC11744.1"
                     /translation="MKLNVGLSQRQTLRQSLVMAPQLIQSLKMLQMPALKLEQKIRQE
                     LAINPMLEEVEELEAEQDVEVEQEAEFDVAEPERAEEKAEVDWDQYLFDDEEGYKVRE
                     TRESDEDRFEGTAAASANLYDHLNEQLMLLKLSEEEQLIGEYIIGNVAPDGYLSISVP
                     EMAKELKTEAQKIQAVLEMIQRFDPTGVAARDLRECLLIQLREKALEGTLAWRIVDEH
                     INELDRKSILQVAKMMNVPVERAQAAMETIRSLSPKPSLGRFDPAAMPVVPDLIVERI
                     GDEFVVYHNDSHIPRLQINSGYKSLMRRGRGASPETREYIKQKLEQARWLLNAINQRR
                     STLIRVMEAIVERQREFFERGQAFLRPLIMEDVAQMVSMNVATISRVSNGKYVQTPLG
                     VYEIKYFFNSGIQRENGDEMSKRSVKQRIEEIIAAEPPDKPLSDQEIYRILNTEGIKL
                     ARRTVTKYREELKIQPARLRKRVG"
BASE COUNT          316 a          663 c          617 g          445 t
ORIGIN      
        1 catgtcgtcg gtctcccccc gcccctgaat gaggttgttg aagacgttga ctttgagttc
       61 ggcgacgcgc cggtggcgct cggcgtcgag caccatctcg gccgagacga tgttgtcaaa
      121 aaagcgggtg aggccgtcca tttcggcctc ggccttcgct ctgatttccg gggtgaggtc
      181 aaagtgacgg gcggttacct ttttcaacat acgctcgctc ctttcccaac agacgcgaca
      241 ggaaatgttc tcgcggctcc atgtatgacg gcctgcccgg ggcaggcgca gccagccgga
      301 ccggggtccg gacacgcact cctcactagt tgatacggtt gtggcgacag agaggttcgg
      361 cagcggccgc tgccgacggt ggcgactgca cttgactgat ccatccgatg gactctctct
      421 cggcgggcgt cgctgcgggc gcccgcggaa acaggccggg tcgttcgctc agcccacccg
      481 tttgcgcagg cgggccggct ggatcttcaa ctcttcacga tacttggtca ccgtacgccg
      541 ggcaagcttt atcccctcgg tgttcagaat gcggtagatt tcctggtccg acaggggctt
      601 gtcgggcggc tcggcggcga tgatctcctc gatccgctgc ttgaccgagc gtttcgacat
      661 ctcatcgccg ttctcgcgct ggatgcccga gttgaagaag tatttgatct cgtacacgcc
      721 gaggggcgtc tgcacgtact tgccgttgga tacccggctg atggtggcga cattcatgct
      781 caccatctgg gcgacgtctt ccatgatgag gggccggagg aaagcctggc cgcgctcgaa
      841 aaactcccgc tggcgctcca cgatcgcctc catcacccgg atcagggtgg accgccgctg
      901 gttgatcgca ttgagcagcc agcgcgcctg ctcgagtttc tgcttgatgt actcgcgtgt
      961 ttccggggag gcgccccgcc cccgccgcat gaggctcttg tagccggagt tgatctgcag
     1021 ccgggggatg tggctgtcgt tgtggtagac cacgaactcg tcgccgatcc gctcgacaat
     1081 gaggtccggc accaccggca tggccgccgg atcgaaccgg cccagggacg gtttcggcga
     1141 gagcgaccgg atcgtctcca tggccgcctg cgcccgctcc accggcacgt tcatcatctt
     1201 ggccacctgg aggatcgact tccggtccag ctcattgatg tgctcgtcga caatccgcca
     1261 cgcgagcgtc ccctccagcg ccttctcccg aagctggatg agcaggcact cccgaagatc
     1321 ccgggcggcc accccggtcg ggtcaaagcg ctggatcatc tccagcaccg cctggatctt
     1381 ctgcgcctcc gtcttcagct ctttggccat ctccgggacg ctgatgctca ggtaaccgtc
     1441 gggcgcgacg ttcccgataa tgtactcccc gatcaactgc tcttcctcgg agagtttcag
     1501 gagcatgagc tgttcgttga ggtggtcgta gaggttggcc gaagccgcgg ccgttccctc
     1561 gaagcggtcc tcatccgact cccgggtttc ccggaccttg tacccctcct cgtcgtcaaa
     1621 gaggtactgg tcccagtcca cttcggcctt ctcctcggcg cgctccggct cggcgacgtc
     1681 gaactcggct tcctgttcga cctcgacgtc ctgttcggcc tcgagctcct cgacctcctc
     1741 aagcatcggg ttgatcgcca gttcctggcg gatcttctgc tcgagtttga gcgccggcat
     1801 ctgcagcatt ttcagcgact ggatcagctg cggcgccatc accagcgact gcctgagcgt
     1861 ctgcctctgg gacaatccga cattgagctt cattgccagt aacccgttat catgcaaacc
     1921 atccgacggt gcggtctgtg cctctctgct aggtgtatcg gaatcgatcg ggcgggtgtt
     1981 tacgaccggg cggccgttcg ttcatttatt agacagacgg tcggttggcg gtccgacccg
     2041 g
//
LOCUS       DAOOKS010000381         2034 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_69635_length_2034_cov_3.468924, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000381 DAOOKS010000000
VERSION     DAOOKS010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69635_length_2034_cov_3.468924"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="PK112_06555"
     CDS             <1..197
                     /locus_tag="PK112_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11745.1"
                     /translation="RVCDLVRAGLPYLATHPVDSRRSVDRRAFKQIHEWYRALHAFNV
                     GDIGPGACAPDADTSVNPRR"
     gene            complement(331..>2034)
                     /locus_tag="PK112_06560"
     CDS             complement(331..>2034)
                     /locus_tag="PK112_06560"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HPC11746.1"
                     /translation="ARGDKFAGVAVSGDQFNQIEDFRASVYRADGKKVYERKLKDMFR
                     TCGFGPSYALYSSNCTYSLDLPGPAYPYTIEYEYAVRSKSLFFLRGAAMQSYLPVVRA
                     LCQVTAPSEPPLHYKVYGLAITPRVVRTGEKTLYEFAAGNLAPLPNESQLPPEAEYPG
                     RVAFSPHAFRLENSAYSGFSWREVGNWYRTLAADRYLAWKAGVTAGDVRQMAREAYER
                     VTGNTRYVAVSIGIGGWQPHAAAETQKLGYGDCKDMTTLLVSYLRQAGVAAYPALIST
                     RGATPIDPDFPAFDFNHVITAALIDGDTLWMDPTCDLCPFGQLPLGDQGMPVLLVTDS
                     GGVLVRTPAAKAEDNVWARRTHLAIGADYRVNLSAEWELCGVPALWLEAMFTASDSED
                     RRLMVGQLLTGDNGGYRVTEASLTRRPADSGGGVAVRVAAVSERPVDLVKGTAYVQPA
                     VYRDFAEVRALDFATRRYPVDLEVPQLMTDEVTVTWPSMGTATAEGPGADSIACGHGF
                     WRRTAAAADSAAVRLTMEAACLGSVVEVGELPTFAEYLKKRQAREREPIRLTASGAAG
                     R"
BASE COUNT          319 a          812 c          590 g          313 t
ORIGIN      
        1 gccgggtgtg tgatctggtg cgggccggcc tgccctacct ggccacccac ccggtggaca
       61 gccgccggtc ggtcgatcgc cgcgccttca aacagatcca tgagtggtac cgcgccctgc
      121 acgccttcaa cgtcggcgac atcggtcccg gcgcctgcgc gcccgacgca gatacctctg
      181 tcaacccgcg gcggtaaccc cgccgctcgc ggctccgcgc gccccgcaac ctccccaggg
      241 cgcgcgcagc gacgtccatc ggcacggcaa cgggcgggtc gcctcacggc cccgcccgtt
      301 gtctttattc gccaagccgc caacggccgc tcaccggccc gccgcccccg aagctgtcaa
      361 ccgtatcggc tcgcgctccc tggcctgccg cttcttcaga tactccgcga acgtcggcag
      421 ctccccgacc tcgaccaccg accctaagca ggctgcctcc atcgtcagcc gcaccgccgc
      481 gctgtccgcg gcggcggccg tccgccgcca gaacccgtgg ccgcaggcga tcgagtccgc
      541 ccccggccct tccgccgtcg ccgtccccat cgagggccag gtcaccgtca cctcatccgt
      601 catgagctgc ggaacctcca ggtccaccgg gtagcgccgg gtcgcaaaat cgagagcgcg
      661 cacttccgca aagtcccggt acaccgccgg ctgcacatag gccgtcccct tgaccaggtc
      721 gaccggccgc tccgacaccg ccgccacgcg caccgcgacc cctccccccg agtccgccgg
      781 cctccttgtc agtgacgcct ccgtcacccg atagccgccg ttgtcgccgg tcagcagttg
      841 ccccaccatc agcctccgat cctcgctatc cgacgctgtg aacattgctt ccagccatag
      901 cgccgggacc ccgcacagct cccactcggc gctgagattc acccggtagt ccgcgccgat
      961 cgccagatgc gtccgccgcg cccacacgtt gtcctccgcc ttcgccgccg gcgtccgcac
     1021 gagaacgccc ccgctgtcgg tcaccaaaag caccggcatc ccctggtcac ccaacggcag
     1081 ttggccgaag ggacagaggt cgcaggtcgg gtccatccac agcgtgtccc cgtcgatcag
     1141 cgccgccgtg atgacgtggt tgaagtcgaa agcggggaaa tcgggatcga tgggcgttgc
     1201 cccgcgtgtc gagatgagcg ccgggtacgc cgccaccccc gcctgccgca ggtacgacac
     1261 cagcagcgtc gtcatgtcct tgcagtcgcc atagccgagc ttctgcgtct cggcggcggc
     1321 gtggggttgc cacccgccga tcccgatcga caccgccacg taccgcgtgt tcccggtgac
     1381 gcgctcatag gcctcgcgcg ccatttgccg cacatcgccc gccgtcaccc cggccttcca
     1441 tgcgagatac cggtcggccg cgagcgtccg gtaccagttc cccacttcgc gccacgagaa
     1501 accgctgtag gcgctgttct ccaggcggaa ggcgtgcggc gaaaacgcca cccgccccgg
     1561 gtactcggcc tccggcggaa gctgcgattc gttcggcagc ggcgccagat tccccgccgc
     1621 aaactcgtat aatgtcttct ccccggtccg caccacccgc ggcgttatgg ccagcccgta
     1681 caccttgtag tgcagcggcg gttcgctcgg cgccgtcacc tggcagagcg cacgcaccac
     1741 cggcaggtaa ctctgcatgg ccgcgccccg caggaaaaac agcgacttcg aacgcaccgc
     1801 atactcgtac tcaatcgtat aggggtaggc cgggccggga aggtcgaggg agtacgtgca
     1861 gttggaggag tagagcgcgt acgacggccc gaatccgcag gtccggaaca tatccttcaa
     1921 cttccgctca taaaccttct tcccgtccgc ccggtaaacg ctcgcccgga aatcctcgat
     1981 ctgattgaac tggtcgccgc tgaccgccac ccccgcgaac ttgtcgcccc gggc
//
LOCUS       DAOOKS010000382         2033 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_69683_length_2033_cov_3.062184, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000382 DAOOKS010000000
VERSION     DAOOKS010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69683_length_2033_cov_3.062184"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(310..>2033)
                     /locus_tag="PK112_06565"
     CDS             complement(310..>2033)
                     /locus_tag="PK112_06565"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HPC11747.1"
                     /translation="FEGRTQLVEELQRKGHIDRIEKYRLAAGTCYRCHSIVEPYLSEQ
                     WFVRMDQLAQPAVEAVKTGKLRFHPEYWSKTYLHWMENIRDWCISRQLWWGHRIPIWY
                     AEDGTVFVSAERPRKEDLPAGCDPEALVQDEDVLDTWFSSWLWPFSTMGWPERTAELE
                     KFYPTKVLSTASEIIFLWVARMVMAGYEFAGELPFSDVYIHGTVRDANGIKMSKSLGN
                     GIDPVEIIEKYGADALRVSLTLATPDGQDPWISKNTFEVGRNFVNKLHQVSRFVMMRL
                     EGRPPVRDTIDESDLVLFDRWILSRLERTIRAVDRNFAEYRLSAATKTLYNFVWNDYC
                     SWYIELIKPDQPGQAIRPGSLDVATYVLDKIVKLLHPFAPFVTERIYLDLIGADLEAV
                     DRKTTIVFGPWPTTQGRFIDDQLESSLEQIQAVVNAVRSVRSELNVPPGRKSDLYVRV
                     DNPQFGDLLQKHIEYFRSLARVENLHCGTAVKRPSPSATAVITGAEIFVPLEGLIDLE
                     VERNRVERELQNLALQLEKVSKKLANQDFLAHAPAEVIEREKAKKADFQDRIEKLNKN
                     LEQIMGW"
BASE COUNT          340 a          724 c          615 g          354 t
ORIGIN      
        1 ccccgcccgc cagaagaagc gcgagtatga ttactgctct gcccatgtgt actcccgtcg
       61 gcggactccg ccgaccctcg ccctcaatat acgcggattc gcggaatggg caatcgcgaa
      121 ccggccccgc cgcgccggcg gtccgggccg gaggcaagct cccgtcttat gtatcggctg
      181 tcccgggccg cgcgcccagc gcggttcgcg gggggcgtct tcgacggccg cgcccggcaa
      241 aaagaaggcc gccgggcctg cggcccggcg gctgccttgt ccccgcgggg cgccgccccg
      301 ccggccctcc taccagccca tgatctgctc gaggttcttg ttcagcttct cgatgcggtc
      361 ctggaaatcc gccttcttcg ccttctcccg ctcgatcacc tcggccggcg cgtgggcgag
      421 aaaatcctgg ttggccagct tcttggacac cttctcgagc tggagggcga ggttctggag
      481 ctcccgctcc acccggttgc gctccacctc gagatcgatc agcccctcaa gcgggacaaa
      541 tatctccgcc cccgtgatga ccgccgtggc cgagggactg ggccgcttga ccgccgtccc
      601 gcaatggagg ttctccaccc gcgccagcga ccggaaatac tcgatgtgtt tctgcagcag
      661 gtcgccgaac tgcgggttgt ccacccgcac gtagaggtcc gacttgcgcc ccggcggcac
      721 gttgagctcc gaacggaccg agcgcacggc gttgaccacc gcctggatct gctcgagact
      781 gctctcgagc tggtcgtcga tgaaccgtcc ctgggtggtg ggccacggac cgaaaacgat
      841 cgtcgtcttc cggtccacgg cctcgagatc ggccccgatc aggtcgaggt agatgcgctc
      901 ggtgacgaag ggcgcgaacg ggtggagcag cttcacgatc ttgtccagaa cgtaggtcgc
      961 gacgtcaagc gagcccggcc ggatcgcctg ccccggctgg tccggcttga tcagctcaat
     1021 gtaccaggag cagtagtcgt tccagacgaa attgtacagc gtcttggtgg ccgccgacag
     1081 ccggtactcg gcgaaattcc ggtcgaccgc ccgaatcgtc cgctccagcc gcgagagaat
     1141 ccaccggtcg aaaaggacca ggtcgctctc gtcgatcgtg tcccgcaccg ggggacggcc
     1201 ctccaggcgc atcatgacaa agcgcgacac ctggtgcagc ttgttgacga aattccggcc
     1261 gacctcgaac gtattcttgc tgatccacgg gtcctgcccg tcgggcgtcg ccagcgtcag
     1321 cgagacccgc agggcgtccg ccccgtactt ctcgataatc tcgaccgggt cgatcccgtt
     1381 gcccagcgac ttggacatct tgatcccgtt ggcgtcgcgc accgtgccgt ggatgtacac
     1441 atccgaaaac ggcagctcgc cggcgaactc gtacccggcc atcaccatgc gggccaccca
     1501 gagaaaaatg atctcgctgg cggtcgagag aaccttggtc gggtaaaact tctccagctc
     1561 cgccgtccgc tccggccacc ccatggtcga gaacggccac agccacgatg agaaccaggt
     1621 gtccagcacg tcctcgtcct gcaccagcgc ctccggatcg cagccggcgg ggagatcctc
     1681 cttccgcggc cgctcggccg acacaaacac cgtcccgtcc tcggcatacc agatcgggat
     1741 gcggtgcccc caccagagct ggcgcgagat gcaccagtcg cggatgttct ccatccagtg
     1801 gaggtaagtc ttcgaccagt actccggatg aaaccgcagc ttgcccgtct tcaccgcctc
     1861 caccgccggc tgcgcgagct ggtccatcct cacgaaccac tgctccgaca ggtacggttc
     1921 gacaatcgag tggcaccggt agcaggtccc ggccgcgagc cggtacttct cgatgcggtc
     1981 gatatgcccc ttgcgctgga gctcctcgac cagctgggtg cgtccctcga acc
//
LOCUS       DAOOKS010000383         2032 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_69730_length_2032_cov_3.610521, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000383 DAOOKS010000000
VERSION     DAOOKS010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69730_length_2032_cov_3.610521"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="PK112_06570"
     CDS             <1..397
                     /locus_tag="PK112_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11748.1"
                     /translation="DRFLMQLGFRYTNHQEQDWFKDHPDEFHLAQYDLNLNLHVYAVP
                     PTKSIVAPYGGVGVQAGLLNIEIAGYDDESDLTILMAADFGLDLKVAEFSKGRSFLTL
                     ASINSYDLFGTEDRPKYLTIGGGLRYWFR"
     gene            501..>2032
                     /locus_tag="PK112_06575"
     CDS             501..>2032
                     /locus_tag="PK112_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11749.1"
                     /translation="MAFGLAAAMAAGGCGSDSPNGPQPQGGQVVALNGVRFQGFMGSD
                     STTTAAQFAVTDAGGNRLVNHQIQVAPVRGDGAAPRSIATDAAGVAVLQYDFSGRMGH
                     AALRLYAEGTDTLEVYFRADAIIPGVHGQVGYVLFDDDYAEVKRWLGQPESEDVLPNR
                     SPVYASWESTRGLVVMLYDWDLDGKLYDSSSVYGAIAVTGFAGTTPDGDAVGMGSTIA
                     EVRKRFGPPTDWGVYPEEPNTLQYYFGLWDLYVFADGSQPDTPAVQMTLGEYIPAFPT
                     EIVPLNGLLYRVANLPGGMTYPALQFAVEDSSGARIGNVDIQLSHAAGAGSFLSTGGG
                     SISYVTTATTPPGLAEFRYLMTPGLSGSAVGRLTIPVIDTLDIYLRRNVLAPGSGGQG
                     QYVLFNETYAEAAAWNGTAASVAGDGRFGPTYAYAAYPDSGLTFVFRDPGGTNQVTSS
                     SQVYGVIAEAPWPGTMASASAAQRGIRIGSTYAEVTGAYGAPSSSATAGDTLGLVYDG
                     YPV"
BASE COUNT          326 a          596 c          758 g          352 t
ORIGIN      
        1 cgaccgcttt ctgatgcagc tcggatttcg ctatacgaac caccaggagc aggactggtt
       61 caaggaccat ccggatgagt tccacctggc gcagtacgat cttaacctca acctgcacgt
      121 gtacgcggtg ccgccgacga agtcgattgt ggcgccgtac ggcggtgtgg gggtgcaggc
      181 gggcttgctc aacattgaga tcgccgggta tgacgacgag tccgatctga cgattctgat
      241 ggcggcggat ttcggtttgg acctgaaggt ggccgagttt tcgaaggggc ggagttttct
      301 cacgctcgct tccatcaaca gctatgacct gttcggcacc gaggaccggc cgaagtacct
      361 cacgatcggc ggcgggctgc gctactggtt ccgctagccg cagacagtga cggcggcggg
      421 cgggccgctg tctggcgaga gacgtgtcaa ggcgcgacgg cgcaagggga actatgagag
      481 gacgtgcggc gagggcggca gtggcgttcg ggctggccgc ggcgatggcg gcgggggggt
      541 gcggcagcga ttcgcccaac gggccgcagc cgcagggcgg tcaggtcgtg gcgctcaacg
      601 gagtgcgttt ccagggattc atggggagcg attcgacgac gacggcggcg cagttcgcgg
      661 tcaccgacgc gggcggaaac cggctggtca atcaccagat acaggtcgcg ccggtgcggg
      721 gggacggagc ggcgccgcga agcattgcga ccgacgcggc cggcgtggcc gtgctgcagt
      781 atgacttcag cggacgcatg gggcacgcgg cactgcggct gtatgcggag gggaccgaca
      841 cgctggaggt ttatttccgg gccgatgcga ttatcccggg ggtccacgga caggtggggt
      901 acgtgctgtt cgacgatgac tacgccgagg tcaagcggtg gctcggccag ccggagtcgg
      961 aggatgtgct tccgaaccgg agccccgtgt acgcgtcctg ggagtccacc cgcggcctgg
     1021 tcgtgatgtt gtacgattgg gatctggatg ggaagctcta tgattcgtcg agcgtctacg
     1081 gagcgatcgc cgtgacggga ttcgcgggga cgacgccgga cggcgacgcg gtcggcatgg
     1141 gctcgacgat cgccgaggta cgcaagcggt ttgggccgcc gacggattgg ggtgtgtacc
     1201 ccgaggagcc gaatacgctc cagtactatt tcggcctgtg ggacctgtac gtttttgcgg
     1261 acggctcgca gcccgacacc ccggcggtgc agatgacgct cggcgagtat atcccggcgt
     1321 ttccgacgga gattgttccc ctcaatggtc tgctctaccg cgtggccaac cttcccggcg
     1381 gcatgaccta tccggcgctg cagtttgccg tggaggattc ctcgggcgcg cggatcggca
     1441 acgtggacat tcagctgtcg catgccgccg gagcgggatc tttcctgtcg accggcggag
     1501 ggtccatctc gtacgtgacg acggcgacga ccccgcccgg tcttgcggaa ttccgatatc
     1561 tcatgacccc cggtctgagc ggaagcgccg tggggcggct cacgataccg gtgatcgaca
     1621 cgctcgacat ctatctgcgg cgcaatgtgc tggcgccggg gtccggaggg caggggcagt
     1681 acgtgctgtt caacgaaacc tacgcggagg cggcggcgtg gaacgggacg gcggcgagcg
     1741 tggccgggga cggccggttc gggccgacct acgcgtacgc ggcctacccc gattccggtc
     1801 tcacgttcgt attccgcgat ccgggcggaa ccaaccaggt gacctcgtcg tcgcaagtgt
     1861 acggggtgat cgccgaggcg ccctggccgg ggaccatggc ctcggcctcc gcggcgcagc
     1921 gcggaatccg gatcggatcg acctatgcgg aggtgaccgg ggcgtacggg gcgccgagca
     1981 gctcggccac ggcgggggac acgctcgggc tggtctacga cggttacccg gt
//
LOCUS       DAOOKS010000384         2029 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_69889_length_2029_cov_2.872340, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000384 DAOOKS010000000
VERSION     DAOOKS010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69889_length_2029_cov_2.872340"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="PK112_06580"
     CDS             complement(<1..228)
                     /locus_tag="PK112_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPC11750.1"
                     /translation="MSGILFLRTGDLERTAAFYIARLDCRRWLDQPGCVILRHDNFLF
                     GLCARERPQTDVLLTFFYDTPAEVDRLYARHR"
     gene            complement(267..668)
                     /locus_tag="PK112_06585"
     CDS             complement(267..668)
                     /locus_tag="PK112_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11751.1"
                     /translation="MSAGGAAVIAAQAKQEQERQEEEVLTTYRSDDLEGWEFKIVRSL
                     TGAFSKPEKVEQLRRDEAQAGWEMIEKFDNNRIRFKRRTDRRANDHLLPFDPYRTNYG
                     IGEGRMAAVVITSMVVVIGLVIVLALLFGDR"
     gene            887..1771
                     /locus_tag="PK112_06590"
     CDS             887..1771
                     /locus_tag="PK112_06590"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HPC11752.1"
                     /translation="MAPLVTIVEWWRRHPRLVQGYTSRRLGNLSYRTAPAAEAAERRV
                     RLAAELGIDPGRMFSIPLGHTNRVVVLRDDSFLARRDRRGYVEPEADEILAWPVLTPE
                     AYAAMPHDREYADGVVFAAANVYALVITADCAAVGFYDPVRQAAGNAHVGLLGAVNEL
                     PKGMVAALVESFGSRPADIEAVIFPCIRPCHYATANSQTWLRIRERVHAAFGEDNPLF
                     ASGYFDLPGMVRSQLRASGLLEEHIFDVGLCTVCNADTLFSHVAAGSAEAQAREGRFG
                     AVMGFRPEAPGGPTGGGR"
     gene            1812..>2029
                     /locus_tag="PK112_06595"
     CDS             1812..>2029
                     /locus_tag="PK112_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11753.1"
                     /translation="MSSMNHDDPPPGLPERLLRYHLPVLLYAGGIIALSSIRDFRPPD
                     LPIPSLDKVVHLLEYAVFAYLVFRSFYH"
BASE COUNT          319 a          675 c          673 g          362 t
ORIGIN      
        1 ccggtggcgg gcgtacaggc ggtcgacctc ggcgggagtg tcgtagaaga aggtgaggag
       61 aacgtccgtc tgtgggcgct cgcgggcgca caagccgaac aggaaattgt cgtggcggag
      121 tatgacgcac cccggctgat cgagccaccg gcggcagtcg aggcgggcga tgtagaaggc
      181 cgctgtccgc tccaggtccc ccgtgcgcaa aaagagaatg ccgctcatgg tgtcagggcg
      241 caaccgctcg cccgagcccg tccctctcag cggtcgccga ataggagcgc gagcacgatc
      301 acgaggccga tcacgaccac catggatgtg ataacgaccg ccgccatccg cccctcgccg
      361 atcccgtagt tcgtgcggta cggatcgaac ggcagcagat gatcgttggc gcggcggtcg
      421 gtgcggcgtt tgaaacggat gcggttgttg tcgaactttt cgatcatctc ccagcccgcc
      481 tgcgcctcgt cgcggcggag ctgctcaacc ttctccggct tgctgaaagc gccggtcagg
      541 gagcggacga ttttgaactc ccagccctcc aggtcatcgg aacgatacgt ggtcaagact
      601 tcctcctcct ggcgttcctg ttcttgcttc gcctgcgccg caatcaccgc ggcccctccg
      661 gcactcattc tgcacctccg gtttgaaaca attacgccgc gtgcgccggg tctattcaag
      721 aaaaaagatt gctggttgac acgaggggac caaatgcgcg agtatcgggg gtggttcggg
      781 aacaggcggg ggcgcccccg ggcggctgca ttcggcgcct tcccggtacg cggcccgctt
      841 gcagggaaaa ggagagatgc gcccgcggcc ggcgggcgac gcggcaatgg caccgctggt
      901 cacgatagtg gaatggtggc ggaggcaccc ccgcctggtg cagggatata cgtcccggcg
      961 gctgggcaac ctgtcctacc gcaccgcgcc ggcggccgag gcggccgagc ggcgggtgcg
     1021 tctggcggcc gaactgggga tcgatcccgg ccggatgttc tcgattcccc tcgggcacac
     1081 caaccgcgtg gttgtgctcc gcgatgattc ctttctcgcc cgccgcgacc gccggggata
     1141 tgtggaaccg gaggccgacg agatcctggc ctggccggtg ctgacccccg aggcgtacgc
     1201 cgcgatgccg cacgaccggg agtatgccga cggggtggtg ttcgcggccg cgaacgtcta
     1261 cgccctcgtg atcacggccg attgcgccgc cgtcggattc tacgaccccg tgcgccaggc
     1321 agccgggaac gcccacgtcg ggctcctcgg ggccgtgaac gaacttccga aggggatggt
     1381 cgcggcgctg gtcgagagtt tcggcagccg cccggccgat atcgaggcgg tgatatttcc
     1441 ttgcatccgg ccctgccatt atgcaaccgc caactcccaa acctggctgc gcatccgcga
     1501 acgcgtgcac gccgccttcg gagaggacaa tcccctgttt gcctcggggt acttcgacct
     1561 gcccggcatg gtgcgctccc aactgcgcgc ctcggggctg ctcgaggagc acatcttcga
     1621 cgtcggcctg tgcacggtgt gcaacgccga taccctcttc tcccacgtgg ccgccggatc
     1681 ggccgaggcc caggcgcggg aaggacgctt cggggcggtc atggggttcc gccccgaggc
     1741 gccgggcggt cccacaggcg gtggccggtg agcgggaatt ttgttgacgc ccggtgcggg
     1801 caggtgtacc catgagctcc atgaatcacg atgatccgcc ccccggcctt cccgaacggc
     1861 tcctccgcta ccatctgccg gtgctcctct acgccggcgg gataatcgcc ctctcttcga
     1921 tcagagactt tcgccctccc gatttgccga tcccctcgct tgacaaggtc gtccatctgc
     1981 tggagtacgc agtgtttgcg tacctcgtgt ttcggtcgtt ctaccatat
//
LOCUS       DAOOKS010000385         2025 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_70108_length_2025_cov_2.644670, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000385 DAOOKS010000000
VERSION     DAOOKS010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70108_length_2025_cov_2.644670"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="PK112_06600"
     CDS             complement(<1..382)
                     /locus_tag="PK112_06600"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HPC11754.1"
                     /translation="MNHMASTAGAPGAAADLSGILRECSGERGDLIPLLQKLQHTAGF
                     ISADGVRQIALALRITENEVYGVASFYSQFRFTPPGRHSIKVCLGTACHVRGGQIISD
                     AVSRELGVAAGETTPDRRFDFQRVA"
     gene            complement(379..1647)
                     /locus_tag="PK112_06605"
     CDS             complement(379..1647)
                     /locus_tag="PK112_06605"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HPC11755.1"
                     /translation="MVQYYHAHQVNPRKCRGHMNCMRRCPTQAIRVRNGTAVIHDELC
                     IDCGTCISVCPSAAIDPVINPLQAFSGFKYKVVVPSSALYAQFDSSIHPWVIHQAFRR
                     LGFDLVVNVGLTCEYLAKATTRYLDKYTGPRPVISSECPCVIRLIQVRYPDLVGNIIR
                     LDVRRELTARGTRRSLMASTGLGSEEIGIIYISPCPAKVVSIEQPAEKEQSWFNGAVA
                     IRDVYALLYPIVMGLQAEFDEGAVPEDFFFRAGWPILGGITQSLNMDNWLAVSGLDHV
                     MKIFDDIENSRLRNVDYVEAVACMMGCVGGIFNIENPYVARANNIRQRTRYEKGRPIP
                     EAEIERRLTDGDFALEHSVPPRPTKYFDTDLETSIKRLREVDRIFQKLHQIDCGVCGA
                     PTCKAFAEDCARGDARLTDCVFLSNRGGAE"
     gene            complement(1680..>2025)
                     /locus_tag="PK112_06610"
     CDS             complement(1680..>2025)
                     /locus_tag="PK112_06610"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPC11756.1"
                     /translation="KSILKKIGLDPKLVRRVAISTYEGEMNVVMHAQSAVATLSITPR
                     CITVVLADRGKGIPDIEKAMQAGWSTATEEMRAMGFGSGMGLPNIKKNADELNVESEV
                     GKGTTLTMRFYI"
BASE COUNT          339 a          707 c          620 g          359 t
ORIGIN      
        1 aggcgacgcg ctggaaatcg aagcgccggt cgggtgtcgt ctcgcccgcc gccaccccaa
       61 gctcgcgcga cacggcgtcg ctgatgatct gccccccgcg cacgtggcag gccgtcccga
      121 gacacacctt gatcgagtgc cgccccggcg gcgtgaagcg gaactgcgag tagaacgagg
      181 ccacaccgta cacctcgttc tccgtgatcc gcagggccag cgcgatctgc cgcaccccgt
      241 ccgccgatat gaacccggcc gtgtgctgaa gtttctgcag cagcgggatg aggtcgccgc
      301 gctcccccga acattcgcgc agtatgccgc tgagatcggc ggccgcgccg ggcgccccgg
      361 ccgtggaagc catgtgattc attccgcacc acctcggttg gaaaggaaca cgcaatcggt
      421 gagccgggcg tccccgcggg cgcagtcttc ggcgaacgct ttgcacgtcg gcgcgccgca
      481 gacgccgcag tcgatctgat gcagtttctg gaatatccgg tcgacctcgc gcaggcgctt
      541 gatggaggtc tcgaggtcgg tgtcgaaata cttcgtcggc cgcggcggca ccgagtgctc
      601 cagcgcaaag tcgccgtcgg tcagacgcct ctcgatctcg gcctccggga tcggccgccc
      661 cttctcgtag cgggtgcgct ggcggatgtt gttcgcccgc gccacgtagg ggttctcgat
      721 gttgaaaatc cccccgacac accccatcat gcaggccacc gcctcgacgt agtcgacatt
      781 gcggaggcgc gaattctcga tgtcgtcgaa tatcttcatc acgtggtcga gccccgacac
      841 cgccagccag ttgtccatgt tgaggctctg cgtgatcccg ccgagaatcg gccatcccgc
      901 ccggaaaaag aaatcctcgg gcaccgcccc ctcgtcgaac tcggcctgga ggcccatgac
      961 gatcgggtag agcagcgcgt acacgtcgcg gatcgccacc gccccgttga accacgactg
     1021 ctccttctcg gccggctgct cgatcgacac caccttggcc ggacacggag atatgtagat
     1081 gatgccgatc tcctccgagc ccaggcccgt cgacgccatc aggctccgcc gcgtcccccg
     1141 cgccgtcaat tcccgccgga cgtccaggcg gatgatgtta cccaccaggt ccgggtacct
     1201 cacctggatg agccggatca cgcacggaca ctcgctcgag atgaccggcc gcggccctgt
     1261 gtacttgtcc aggtagcggg tggtcgcttt cgccaggtac tcgcaggtga gcccgacatt
     1321 gaccacgaga tcgaacccga gccgccggaa cgcctggtga atgacccacg ggtggatgct
     1381 cgaatcgaac tgggcgtaga gagcggagga ggggacgacg actttgtact tgaatccgct
     1441 gaacgcctgg agcgggttga tgaccgggtc gattgcggcc gaggggcaga ccgagatgca
     1501 cgtgccgcag tcgatgcaga gttcgtcgtg gatgaccgcc gtgccgttgc ggacccggat
     1561 cgcctgcgtc gggcagcgcc gcatgcagtt catgtgcccg cggcacttgc ggggattcac
     1621 ctggtgggca tggtagtact ggaccacggc gcgtcctttc actcgcgcgc gacgcgcgct
     1681 cagatgtaaa accgcatggt cagcgtcgtg cctttgccga cctcgctctc aacgttcaac
     1741 tcatccgcgt tcttcttgat attgggcaat cccatgcccg acccgaaccc catcgcccgc
     1801 atctcttcgg tcgccgtcga ccaccccgcc tgcatcgcct tctcgatgtc cggaatgccc
     1861 ttcccccggt cggccagcac caccgtgatg cagcgcggcg tgatcgacag cgtggccacc
     1921 gccgactgcg cgtgcatgac cacgttcatc tccccctcgt acgtggaaat cgccacccgc
     1981 cgcacgagct tcgggtccag gccgatcttc ttcaggatcg acttc
//
LOCUS       DAOOKS010000386         2023 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_70207_length_2023_cov_2.877033, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000386 DAOOKS010000000
VERSION     DAOOKS010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70207_length_2023_cov_2.877033"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="PK112_06615"
     CDS             <1..352
                     /locus_tag="PK112_06615"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPC11757.1"
                     /translation="PGNVRELENEIKRLALLAGDRRVIGPDIVSGKLRAVAEQVESAL
                     PALQAGADVEFTETYSLYDYLAEHEKQFIIRALKEKGGIEKHAAAILNIPESTLRLKI
                     KQYNIDPKHLDAVN"
     gene            591..1268
                     /locus_tag="PK112_06620"
     CDS             591..1268
                     /locus_tag="PK112_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HPC11758.1"
                     /translation="MLWGFAPLDRHDWALENVLVLLVAPVLVLSYRRFPLSRVSYTLL
                     FVFLCIHEIGAHYTYAEVPYDAWFRSLFGAGLNALLGWDRNNFDRIVHFSYGLLLAYP
                     IREVFCRVAAAKGFWSYFLPLDVTMSTSMLFELFEWWAAELFGGDLGVAYLGTQGDVW
                     DAHKDMGLATTGAVIAMLVTAAINLAIQSDFAREWRESLAVKHRRPLGEVEMARLRRK
                     KQRRPSP"
     gene            1330..>2023
                     /locus_tag="PK112_06625"
     CDS             1330..>2023
                     /locus_tag="PK112_06625"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11759.1"
                     /translation="MARRNAISCGMIRALCYTHPALDPFRNMAVDEWLFAQAQRRPGM
                     VVLRLYTWRTGAITFGCNQRLDHAVVHEALGGTPLIRRVTGGRALYHDPTELTYAVVV
                     NAEGLPPGPLVGSVARTSAAIAEALTAFLAELGIRSQYLRSSVGGGSHPAFFHTAPCF
                     ASAARHEVVADGRKLAASAQRRMGHTLLQHGAIKPGGVAYHPALDGPVCPPERTVAPD
                     PVWAQHLHTWSGI"
BASE COUNT          305 a          666 c          689 g          363 t
ORIGIN      
        1 gccggggaac gtgcgcgaac tcgagaacga gatcaagcgg ctggcgctgc tggcgggcga
       61 ccgccgggtg atcggcccgg acatcgtgtc gggcaaactc cgcgccgtgg ccgagcaggt
      121 cgagagcgcc ctgccggcgc tccaggccgg cgccgatgtc gagttcaccg agacctactc
      181 cctctacgac tacctcgccg agcacgagaa gcagttcatc atccgggccc tgaaagagaa
      241 gggcgggatc gagaagcatg cggcggcgat tctcaacatt cccgagtcga cgctgcggct
      301 gaagatcaag cagtacaata tcgatccgaa gcacctggac gccgtgaact agcgtccggg
      361 ccggaggcaa gacaagagac ggccgcccca ctgtcgccgg tggggcggtt ttttgtcggg
      421 cgccgaccca gaccttgaca cgcgcggcgc aggttcggta ttatggcggg cggcgcgccg
      481 gctgcctgcc ggcgtgtcgc agaaccgcaa tccacggagg tgcccgcctg aataccagag
      541 cctacacccg ctatgtcttc accctcgggg cgctcttcgc cgcctgggtc gtgctctggg
      601 gattcgcccc gctcgatcgc cacgactggg ctttggagaa cgtgctcgtc ctcctcgtgg
      661 cgccggtgct cgtgttgtcc taccggcgtt tccctctctc ccgcgtctcc tacacgctgc
      721 tcttcgtatt tctctgcatc catgaaatcg gggcgcacta cacttacgcc gaagtgccgt
      781 acgacgcgtg gttcaggagt ctattcggcg ccgggctcaa cgccctgctc gggtgggacc
      841 gcaacaactt cgaccgcatc gtgcacttct cctatggcct cctgctggcc tacccgatcc
      901 gggaagtttt ctgccgggtg gccgccgcga aaggcttctg gtcgtacttc ctgccgctgg
      961 acgtgacgat gtcgacgtcg atgctgttcg agctgttcga gtggtgggcg gccgagctgt
     1021 tcggcggaga cctgggggtg gcgtacctcg gcacgcaggg ggatgtgtgg gatgcacaca
     1081 aggacatggg gctggcgacc accggggcgg tgatcgcgat gctggtgacg gcggccatca
     1141 atctcgccat ccagtccgac tttgcgcgcg agtggcggga aagcctggcg gtgaaacacc
     1201 ggcggccgct tggcgaagtc gagatggctc gcctgcgccg caagaagcag cgccgcccat
     1261 cgccgtgagg gtcggagcga cggtcccgcc gcggggtctt ttggacccag ggcgccggcg
     1321 agggcttgtt tggcgcgtcg caacgccata tcatgcggca tgatccgcgc gctctgctat
     1381 acccacccgg ccctcgatcc cttccgcaat atggcggtcg acgaatggct gttcgcacag
     1441 gcgcagcggc ggccggggat ggtcgtcctg cggctctaca cgtggcgcac gggggcgatc
     1501 acgttcggct gcaaccagcg gctcgaccac gcggtggtcc acgaggcgct gggcggcacg
     1561 ccgctcattc gccgggtgac cgggggacgg gcgctctatc atgatccgac cgagctcacg
     1621 tacgcggtgg tggtgaacgc tgagggtctt ccgccgggac cgctggtggg atcggtcgcc
     1681 cgcacttcgg ccgccatcgc cgaggcgctc accgcttttc tggcggagct ggggatccgg
     1741 tcgcaatatc tgcgatcttc ggtcggcggc ggaagtcatc ccgccttctt tcacacggcc
     1801 ccctgtttcg cctcggccgc gcggcacgag gtggtggccg acggccgcaa gctggcggcg
     1861 tcggcgcagc gccgcatggg gcatacactt ctgcagcacg gcgcgatcaa gccgggcggc
     1921 gtcgcctatc atccggcgct cgatggcccg gtatgtccgc ccgaacgcac ggtcgcccct
     1981 gatcctgttt gggcgcaaca tcttcatacc tggagcggaa tct
//
LOCUS       DAOOKS010000387         2018 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_70423_length_2018_cov_3.306164, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000387 DAOOKS010000000
VERSION     DAOOKS010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70423_length_2018_cov_3.306164"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..415)
                     /gene="nuoK"
                     /locus_tag="PK112_06630"
     CDS             complement(113..415)
                     /gene="nuoK"
                     /locus_tag="PK112_06630"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HPC11760.1"
                     /translation="MVPVSAYLIVAAVLFGIGTIGVLASRNMIILIMSIELMLNAANL
                     ALIAFSRALNVMDGHAFVFLVLTVAAAEAAVGLAMVLTVFRNRDSINIDRFNLLKW"
     gene            complement(417..1022)
                     /locus_tag="PK112_06635"
     CDS             complement(417..1022)
                     /locus_tag="PK112_06635"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HPC11761.1"
                     /translation="MSLDAIIFVLSAVVAVFGATMMIVQRNPVASVLYLIVSLIAQAV
                     LYIQLGTAFMGAVLVIVYAGAILVLFLFVIMLLNLRGREDLGEGSAPLSRLTKYLVSL
                     LFVVELVFVIRAGFFAETAVVGVFAGGAVADDFGSVEHTAGLLFTRYLYPFELTAALL
                     LAAVVGAVAIAKRDRAEGPPAGAETRGRAEDAPPQTHKVES"
     gene            complement(1019..1525)
                     /gene="nuoI"
                     /locus_tag="PK112_06640"
     CDS             complement(1019..1525)
                     /gene="nuoI"
                     /locus_tag="PK112_06640"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004538.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HPC11762.1"
                     /translation="MKDVLTALTNAVIKTPKGLLITLKHLFKKPVTVDYPRQKRPMAP
                     RYRGRHYLERYDDGTERCVCCGLCAAACPADAIYMEPEENEKGERRARTYEINLLRCI
                     FCGYCEEACPEEAIFLGQDYEFSSSDKSSFIWKKDDMLVAHPRRDNPFKRLYRRVRKV
                     YGVADKVR"
     gene            complement(1544..>2018)
                     /locus_tag="PK112_06645"
     CDS             complement(1544..>2018)
                     /locus_tag="PK112_06645"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HPC11763.1"
                     /translation="ILIANTLSMRELVEQQASIADWFIWKQPLGFLLYLTCAVAETNR
                     APFDLPEAESELVAGYHTEYSSMRFATFFISEYGAMIAVSCVGTTIFFGGWQGPFLPP
                     FLWFFLKVLAFMFLYIWLRATFPRFRYDQLMAFGWKVLFPLALLNSLVTAAFVML"
BASE COUNT          418 a          627 c          656 g          317 t
ORIGIN      
        1 ggcgttgacg gcggggcggc ggagtcggcc caggagcagc ccgttgacga cgaagccgat
       61 cagcggcaaa agcggtatga gaaacaggta atccgacata gcgtccccgg ccctaccatt
      121 tcaacaggtt gaaccggtcg atgtttatcg agtcccggtt gcggaacacg gtgagcacca
      181 tggccagtcc cacggccgcc tcggccgcgg ccacggtcag cacgaggaac acgaacgcgt
      241 ggccgtccat gacattgagc gcgcgcgaga aggcgatcag ggcgaggttg gccgcgttga
      301 gcatgagctc gatgctcatg atcaggataa tcatgttgcg ggaggcgagc acgccgatcg
      361 tgccgatccc gaagagcacg gccgcgacaa tcaggtatgc ggaaaccgga accatgctag
      421 gactccacct tgtgcgtttg cggcggggcg tcctcggcgc ggccgcgcgt ctcggcgccg
      481 gccggcggcc cctccgcccg gtcgcgcttg gcgatcgcca ccgctccgac gaccgccgcc
      541 aacagcagcg ccgccgtcaa ttcgaaggga tacaggtagc gcgtgaacag gaggccggcc
      601 gtgtgctcca ccgaaccgaa atcgtcggca accgccccgc cggcgaagac gccgacgacg
      661 gcggtttcgg cgaagaatcc cgcccggatg acaaacacca gttcgaccac gaagagcagc
      721 gagacgaggt acttggtcag ccggctgagc ggggccgacc cctcgccgag atcctcccgc
      781 ccgcggagat tgaggagcat gatgacgaag aggaacaaaa cgaggatagc gccggcatag
      841 acgatcacga ggacggcgcc catgaaggcc gttccgagct ggatgtacag gacagcctgg
      901 gcgatgagcg agacgatcag gtagagcacc gaggccaccg ggttgcgctg gacgatcatc
      961 atggtggcgc cgaacacggc caccaccgcc gagagcacga atatgattgc gtcgagactc
     1021 atcgcacctt gtccgccacc ccgtacacct tgcgcacccg ccggtagagt ctcttgaagg
     1081 ggttgtctct gcgcgggtgc gcgacgagca tgtcgtcttt cttccagata aatgaactct
     1141 tgtcgctgct ggagaattca tagtcctggc cgaggaagat cgcttcctcg gggcaggctt
     1201 cctcgcagta gccgcagaag atgcagcgga ggaggttgat ttcgtaagtg cgggcgcgcc
     1261 gctccccctt ctcgttctcc tccggttcca tgtagatggc gtcggccgga caggcggcgg
     1321 cgcacagacc gcagcagacg caccgctcgg tgccgtcgtc ataccgttcg aggtagtggc
     1381 ggccgcggta gcgcggcgcc atcggccgct tctgccgggg ataatcgacc gtcaccggct
     1441 tcttgaacag atgtttcagc gtgatcagca ggcccttcgg ggtcttgatc acggcgttgg
     1501 tcaaggcagt cagtacgtcc ttcatggcat gcgacctctc tccttagagc atcacaaacg
     1561 ccgccgtcac cagcgagttg agcagcgcca gcgggaacag cactttccac ccgaacgcca
     1621 tgagctggtc atagcggaac cgggggaagg tcgcccgcag ccaaatatag aggaacatga
     1681 acgccagcac tttcaaaaag aaccagagga acggcggcag aaacggcccc tgccagcctc
     1741 cgaagaagat cgtcgtcccc acgcaggaga cggcgatcat ggccccgtac tcgctgatga
     1801 agaaggtggc aaagcgcatc gacgagtact ctgtgtggta tcccgccacg agctcgctct
     1861 cggcctcggg gaggtcgaac ggggcgcggt tggtctcagc caccgcgcag gtcaggtaga
     1921 ggagaaaccc gagcggctgc ttccagatga accagtcggc gatcgacgcc tgctgctcca
     1981 ccaactcgcg catcgagagg gtgttggcga tcaggatt
//
LOCUS       DAOOKS010000388         2015 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_70588_length_2015_cov_3.777551, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000388 DAOOKS010000000
VERSION     DAOOKS010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70588_length_2015_cov_3.777551"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1285
                     /locus_tag="PK112_06650"
     CDS             <1..1285
                     /locus_tag="PK112_06650"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HPC11764.1"
                     /translation="KAYLPPKPQVYSKKQNAQDAHEAIRPTYLALPPDKVKRFLNARQ
                     FKLYSLIWNRFVASQMNPARYDVETIDIAAGRFLFRAAAQRLTFDGYLKIYHEEVEAD
                     GNGNGNGAAAALPRLAEKDPLRLLELRPNQSFTKPPARYSEAALVKRLEADGIGRPST
                     YASIISTLRDRKYVKLEQRKLAPTELGLTVNKILVENLPKLFNVKFTAEMEKELDLIE
                     DGTDDWVKVLNEFYEPFKKSIEVLKGRQKEIKEAMTEETDIKCEKCGSPMVIKWGRNG
                     RFLACSAYPECKSTRPLPEEEAARKTDEVCEKCGAPMVIKTGRFGRFLACSAYPNCKN
                     TKAITLGIACPQDGCKGQLVEKRTRGGKIFYGCSAYPSCKFATWDKPVPHPCPHCAYP
                     FLVAKESKAKGEYLQCPRCKAVIAPDSVEAHTPAH"
     gene            1405..>2015
                     /locus_tag="PK112_06655"
     CDS             1405..>2015
                     /locus_tag="PK112_06655"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HPC11765.1"
                     /translation="MLQQDLTAYLGALAEERRCSPRTVEAYRRDLEPWLAFLAEQHAA
                     LPGGRPNDPLLLRVYLRRRAEAGVSNRSLARFLSALSGFQKFLAARGRAEVWLFKLPR
                     MKYPARMPRFLPQKEAGRLFEEPPAQQPERTYFYRRDFLMIALLYATGIRREELAGIR
                     PGDLDLRQGLITVTGKGNKVRVVPIGDATLKDVRAYLDDRARFL"
BASE COUNT          382 a          726 c          579 g          328 t
ORIGIN      
        1 caaggcctac ctgccgccca aaccgcaggt gtactcgaaa aagcagaacg cccaggacgc
       61 ccacgaggcg atccggccca cctatctcgc gctcccgccc gacaaggtca agcgcttcct
      121 caacgcccgc cagttcaagc tctacagcct gatctggaac cgcttcgtcg cctcgcagat
      181 gaacccggcc cgctacgacg tcgagaccat cgacatcgcc gccggccgct tcctcttccg
      241 ggccgccgcg cagcggctca cgttcgacgg ctacctgaag atctaccatg aggaagttga
      301 ggcggatggc aacggcaacg gcaacggcgc cgccgcggct ctgccccggc tggccgaaaa
      361 ggatcccctc cggctcctcg agctccggcc gaaccagtcg ttcacgaagc cgccggcccg
      421 ctactccgag gccgccctgg tgaagcggct tgaggccgac ggcatcggcc gcccctccac
      481 ctacgcctcc atcatttcca ccctccgcga ccggaaatat gtcaagctcg aacaacgcaa
      541 gcttgcgccg accgaactcg gcctgacggt gaacaagatc ctcgtggaaa accttcccaa
      601 actcttcaac gtcaaattca ccgccgagat ggagaaagag ctcgacctca tcgaagacgg
      661 caccgatgac tgggtgaagg ttctcaatga gttctacgag cctttcaaga aatccatcga
      721 ggttctcaag ggacggcaga aggaaatcaa ggaggcgatg accgaggaaa ccgacatcaa
      781 gtgcgagaag tgcggttcgc cgatggtcat caagtggggg cgcaacggcc ggttcctcgc
      841 ctgctccgcc taccccgagt gcaagtccac ccgcccgctt ccggaagaag aggctgcccg
      901 caagaccgat gaagtgtgcg agaagtgcgg agcgccgatg gtcatcaaga ccggccggtt
      961 cggccgcttc ctcgcctgct cggcctaccc gaactgcaag aataccaagg cgatcacgct
     1021 cggtatcgcc tgcccgcagg acggctgcaa aggacagctt gttgaaaaac gcacccgcgg
     1081 cggcaaaatc ttctacggct gttccgccta cccgtcgtgc aagttcgcca cctgggacaa
     1141 gccggtgccg cacccctgcc cgcactgcgc ataccccttc cttgtggcca aggaatccaa
     1201 ggcgaagggc gaatacctcc agtgcccccg gtgcaaggcg gtgatcgccc cggactcggt
     1261 cgaggcccac accccggcac actaattcgg aatccccgat cggcttcggt cgccccgggc
     1321 cgggcccgcg ctcgaatccg gccgcccggt tgccatcaat tgtattgacc tccctccgac
     1381 cgccccgtat ttatactccc gcgtatgctc cagcaggatc tgaccgcata tttgggcgct
     1441 ctcgccgagg agcgccgctg ttcccctcgg actgtcgagg cctaccgccg cgaccttgaa
     1501 ccgtggctgg cttttctggc cgagcagcac gccgccctgc cgggaggccg gccgaacgac
     1561 ccgctcctgc tgcgcgtcta cctgcggcgg cgggccgaag ccggcgtgtc gaaccgctcg
     1621 ctcgcccgtt ttctgtcggc cctctccggt ttccagaaat tcctggctgc ccggggaaga
     1681 gccgaggtct ggctgttcaa actcccgcgg atgaagtacc cggccaggat gccccggttc
     1741 cttccgcaga aggaggccgg gcggctgttc gaggaaccgc cggcccaaca gccggagcgc
     1801 acctatttct accggcgcga ctttctcatg atcgccctcc tgtacgccac cggcattcgc
     1861 cgcgaggagc tggccggcat tcggccgggg gatctcgacc tccgccaggg gctcatcacg
     1921 gtcaccggca aggggaacaa ggtccgcgtc gtccccatcg gcgacgcaac gctcaaggat
     1981 gtgcgcgcct acctcgatga ccgcgcccga tttct
//
LOCUS       DAOOKS010000389         2011 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_70791_length_2011_cov_3.385481, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000389 DAOOKS010000000
VERSION     DAOOKS010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70791_length_2011_cov_3.385481"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="PK112_06660"
     CDS             complement(<1..357)
                     /locus_tag="PK112_06660"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPC11766.1"
                     /translation="MYRLSIDNLAKRFGSRKVFQDISIELATGQSLAVIGPNGSGKST
                     LVRCLLGLHRPSKGSVAYYETGDGEGAGRRLGEEEFRARAAFVSPYLNLYGQLSGEEN
                     IRFFAAMRGLPLTGKQI"
     gene            complement(523..981)
                     /locus_tag="PK112_06665"
     CDS             complement(523..981)
                     /locus_tag="PK112_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11767.1"
                     /translation="MSKTSFTQADAERIAKVVAAKSVGFETDHYRFKLDNPEERRSLV
                     LEVYPETSLGKQRGMLVVVYAGNSHLQLHNCTGYVVSEDLGEVTFVAETSERLSGLVV
                     ERGAACSQYAAINRDLISSDFTQLGVAVMLSGVALSLAEEILEERKKNDR"
     gene            complement(1013..1342)
                     /locus_tag="PK112_06670"
     CDS             complement(1013..1342)
                     /locus_tag="PK112_06670"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HPC11768.1"
                     /translation="MPFGIGWAELLVIFAIVLLLFGARRLPEIAQGLGKGIREFKRAM
                     KDTTDEVRGSLDTEGTRDYKRAIPGSADREARRDDRERDDRARDTREQGDRERDDRPD
                     DPPDRRR"
     gene            complement(1380..1649)
                     /locus_tag="PK112_06675"
     CDS             complement(1380..1649)
                     /locus_tag="PK112_06675"
                     /inference="COORDINATES: protein motif:HMM:NF025572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4321 domain-containing protein"
                     /protein_id="HPC11769.1"
                     /translation="MKKREVTFILVALILGAVVGGLLGDIVAGFLPEDSEVAKVFGKS
                     IEIGVKTIQVDFYAIAFTFGLMLKVNFMSVLVLLLVIIYFRWWYL"
     gene            complement(1649..>2011)
                     /locus_tag="PK112_06680"
     CDS             complement(1649..>2011)
                     /locus_tag="PK112_06680"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HPC11770.1"
                     /translation="VYLRVEHTSSPYTRACLRGEVLRIPIAHGEGNYYHFDREIGRLE
                     DRGQVVFRYCDREGNTTPAANPNGSRNNIAGIANEEGNVLGLMPHPERAVEPILGSSD
                     GLRIFESIRATIGTTVRT"
BASE COUNT          328 a          660 c          653 g          370 t
ORIGIN      
        1 gatctgcttg ccggtgagcg ggagcccgcg catcgcggca aagaacctga tattctcctc
       61 tccggacaac tggccgtaga gattgaggta gggcgacacg aaggcggccc gcgcccggaa
      121 ttcctcctcg cccagccggc ggccggcccc ctccccgtcc ccggtctcgt agtacgccac
      181 cgatcccttg ctcgggcggt ggagcccgag caggcagcgc acaagggtcg atttgcccga
      241 gccgttgggg ccgatcaccg ccagcgattg gccggtggcc agctcgatcg agatgtcctg
      301 gaacaccttt cgggacccga accgtttggc caggttgtca atggacagcc ggtacatggg
      361 ggcaatttgg ggaatccgat ccgggatgca agcggaaagg tgcggggcgg gtgtccggcg
      421 gcgcggcgat ggtccgggcg gacccgcatg agtccgccgc cgcggcacga aaaagccgaa
      481 gcctccgggg cttcggccgg cgccgattct gtcccggccg cctcaccggt cgttcttctt
      541 gcgctcctcg agaatctcct cggccagcga gagagccacc ccggagagca tcacggccac
      601 gccgagctgc gtgaaatcgg aggaaatgag gtcgcggttg atcgccgcgt actgcgagca
      661 ggcggcgccg cgctcgacga ccagcccgga gaggcgctcg gacgtttcgg ccacgaacgt
      721 gacctcgccg agatcctcgg ataccacgta cccggtgcag ttgtgcaact gcaggtggga
      781 gttgccggcg tacacgacca cgagcatccc gcgctgtttg ccgagcgacg tttccggata
      841 gacttcgagg acgagggagc gccgctcctc ggggttgtcg agtttgaagc ggtagtggtc
      901 ggtctcgaat ccgaccgact tggccgccac caccttcgca atcctctcgg catccgcctg
      961 tgtgaacgat gtcttgctca tggctgttac cgctttctcc gctcggcccg ggctagcgcc
     1021 ggcggtctgg ggggtcgtcg gggcggtcgt cccgctcgcg gtcgccctgt tcgcgggtgt
     1081 cgcgcgcgcg atcgtcccgc tcgcggtcgt cgcggcgggc ctcgcggtcg gcgctgcccg
     1141 gaatggcgcg cttgtaatcc cgcgtaccct cggtgtccag cgaacccctg acctcgtcgg
     1201 tcgtgtcttt catggcccgc ttgaattcgc ggatgccttt gcccaggccc tgggcgatct
     1261 ccggcaaccg cctcgccccg aacaggagca ggacgatcgc gaagatgacc agcagttccg
     1321 cccaaccgat tccaaacggc atgttaaccg ctcactttcc agagttccgg tggccgtgtc
     1381 tagagatacc accacctaaa gtatatgatg acgagcagaa gcacaagcac ggacatgaaa
     1441 ttgactttga gcatcaggcc gaacgtaaag gcgatggcgt agaagtcgac ctggatcgtc
     1501 ttgaccccga tctcgattga tttgccgaat actttcgcga cttccgagtc ctccggcagg
     1561 aaaccggcga cgatgtcgcc gaggaggccg ccgacgacgg cgccgaggat gagcgccacc
     1621 aggataaagg taacctcgcg tttcttcatc aggtccgcac cgttgttccg atcgttgctc
     1681 ggatcgattc gaatattctc agtccgtcgg acgagccgag gatcggctcc accgcccgct
     1741 ccgggtgcgg catcagcccg aggacgttcc cttcctcgtt ggcgatgccg gcgatgttgt
     1801 tgcgtgagcc gttaggattg gccgccggcg tcgtgtttcc ctcccggtcg cagtagcgga
     1861 acaccacctg cccccggtct tccagccgcc cgatctcgcg gtcgaaatgg tagtagttcc
     1921 cctcgccgtg ggcgatcggg atcctgagca cctcgccgcg caggcaggcg cgcgtgtagg
     1981 ggctgctggt gtgctcgacc cgcaggtaca c
//
LOCUS       DAOOKS010000390         2010 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_70851_length_2010_cov_2.660358, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000390 DAOOKS010000000
VERSION     DAOOKS010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70851_length_2010_cov_2.660358"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="PK112_06685"
     CDS             complement(<1..866)
                     /locus_tag="PK112_06685"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPC11771.1"
                     /translation="MRFIYSEWNEELFRRLRQLADLMAIYNHLLIRLAGDVEETLRLM
                     RRLQEQGILDEKYDLDEFEEELRRNRLIGTGRDRRLRLTGKGERRLRQDAFDAVFQGM
                     KAAGRGNHPLPYEGGSAEEPLPERRPFEFGDSPRRIDYTSSLFNSVLRTGDLGLDMAE
                     RDLEVNEAERLASCATVLMIDISHSMILYGEDRFTPAKQVAMAFTELILTRYPKDDLT
                     VVLFGDTARQVPIEELPYVSVGPYHTNTQAGLRMARRLLMAKKHANRQIFMITDGKPS
                     MITRSNGRLYRNP"
     gene            complement(900..>2010)
                     /locus_tag="PK112_06690"
     CDS             complement(900..>2010)
                     /locus_tag="PK112_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HPC11772.1"
                     /translation="HRSERYHEKLATPDVSIADLIDDIDPIKAAREKLDISNEEVIHW
                     GIIPRTNRGIFAINELPDLQPRIQVGLLNILEEKDIQVRGFPLRVPLDMLLVFTANPE
                     DYTNRGNIITPLKDRIDAQIITHYPRTLAEARAITEHEADIAAADIRIPDLVRDLIEE
                     VSFQARASEFVDQTSGVSARVSIAAYEALVSNIERRVLRFRDQDRFPRMADVYAIIPA
                     VTGKIELVYEGEREGSALVAHNLIGLAVKEVFARHFPEPRRERRRGDQSSAADRENPY
                     EAITDYFSSGRKLELSDDMPFDRYKKALTSIAGLDRIVRRYFDTRDEREFLLRMELVL
                     EGLHQYNVIAREIRDSAVSYGDVLSDMLRGMESDR"
BASE COUNT          304 a          578 c          752 g          376 t
ORIGIN      
        1 ggattgcgat agagccggcc gttggaccgg gtgatcatcg acggcttgcc atcggtgatc
       61 atgaagatct gccggttggc gtgcttcttg gccatgagca gccggcgggc catgcgtagg
      121 ccggcctgcg tgttggtgtg gtaggggccg acgctcacgt agggcagctc ctcgatcggc
      181 acctgccggg cggtgtcgcc gaagagaacg acggtgagat cgtccttggg gtagcgcgtg
      241 agaatcagtt cggtgaaggc catggccacc tgcttggcgg gagtgaagcg atcctcgccg
      301 tagaggatca tcgaatgcga aatgtcgatc atgaggacgg tcgcacagga ggcgaggcgc
      361 tcggcctcgt tgacttcgag gtcgcgctcg gccatgtcga ggccgaggtc gccggtgcgg
      421 agaacggagt tgaagagcga gctcgtgtaa tcgatgcggc gggggctgtc gccgaattcg
      481 aaggggcggc gttcgggcag cggctcctcc gccgagcccc cctcgtacgg cagcgggtgg
      541 ttgccccggc cggccgcttt catcccctgg aacacggcgt cgaaagcgtc ctggcggagc
      601 cggcgctccc ccttgccggt caggcggagc cggcggtcgc ggccggttcc aatgaggcgg
      661 ttccggcgga gttcctcctc gaactcgtcg aggtcgtact tctcatcgag gattccctgc
      721 tcctgcaggc gccgcatgag ccggagggtt tcctcgacgt cgccggcgag ccgtatgagc
      781 aggtggttgt agatggccat gaggtcggcc agctggcgga gccggcggaa cagctcctcg
      841 ttccattcgg agtatatgaa tcgcatggcc tctcccccgg gcggcggggc ggcgcccggt
      901 cagcggtcgc tctccatccc gcgcagcatg tccgagagca cgtcgccgta gctgaccgcg
      961 gaatcgcgga tttcgcgggc aatcacgttg tactggtgca ggccctcgag caccagctcc
     1021 atgcgcagga gaaactcccg ttcgtcgcgg gtgtcgaaat accgccgcac gatgcggtcg
     1081 agcccggcaa tcgaggtcag cgctttcttg taccggtcga acggcatgtc gtcggagagc
     1141 tcgagtttgc ggccggacga gaagtagtcg gtgatggcct cgtaggggtt ttcccggtcg
     1201 gccgccgagc tctggtcgcc gcggcgcctt tcccggcgcg gctcggggaa gtgccgggca
     1261 aagacctcct tgaccgccag cccgatcagg ttgtgggcca cgagcgcaga accctcccgc
     1321 tccccttcgt acaccagttc gattttgccg gtgacggccg ggatgatggc gtagacgtcg
     1381 gccatgcggg ggaagcggtc ctggtcgcgg aagcggagga cgcggcgctc gatgttcgag
     1441 acgagagcct cgtaggcagc gatggagacg cgggcggaga cgcccgaggt ctggtcgaca
     1501 aattcgctgg cgcgggcctg gaacgacacc tcctcgatga ggtcgcgaac gaggtcgggg
     1561 atgcggatgt cggcggcggc gatgtcggcc tcgtgctcgg tgatggcacg ggcctcggcc
     1621 agcgtgcgcg ggtagtgggt gatgatctgt gcgtcgatgc gatctttgag cggggtgatg
     1681 atgttcccgc ggttggtgta gtcctccggg ttggcggtga acaccaggag catgtcgagc
     1741 ggcacgcgca gggggaaacc gcggacctgg atgtcctttt cctcgaggat gttcaggagc
     1801 cccacctgga tgcgcggctg gaggtcggga agttcgttga tggcgaaaat cccccggttg
     1861 gtgcggggga ttatgcccca gtggatgacc tcctcgttgg agatgtcgag cttctcgcgg
     1921 gccgccttga tcggatcgat gtcgtcgatg aggtcggcga tggagacgtc gggcgtggcg
     1981 agcttctcgt ggtagcgctc gctgcggtgg
//
LOCUS       DAOOKS010000391         2007 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_70981_length_2007_cov_3.532787, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000391 DAOOKS010000000
VERSION     DAOOKS010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70981_length_2007_cov_3.532787"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="PK112_06695"
     CDS             complement(<1..211)
                     /locus_tag="PK112_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019856144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HPC11773.1"
                     /translation="MSKPITVTDDSFEQEVIQADKPVIVDFWATWCGPCKMIAPILEE
                     VAREYADRVKVAKIDVDSNTEVARKF"
     gene            complement(353..>2007)
                     /locus_tag="PK112_06700"
     CDS             complement(353..>2007)
                     /locus_tag="PK112_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="CoA activase"
                     /protein_id="HPC11774.1"
                     /translation="RPYNGFDRRLSLDLPDKLRNLGYRAIPIDFLPAEQADSEFPYMY
                     WRYGRRILQAADFIRKHPGLFAVYITSFGCGPDSFITHFFRKQMAGKPYLQLELDEHS
                     ADAGLVTRCEAFVDSLRFYRYTPPVGSFSVGRDEFRPFEKTIYIPNMCDHAYALRAAF
                     ARYGLTAEVLDEPDELTLEYGKKFTSGKECFPCVVTTGEFIRRVRRPDFDRKRSVFFM
                     PGADGPCRFGLYSPFHRMVLDDLGFTDVPIYSPNSKDGYEDGFGLQGTDFRKTAWRGL
                     VFVDGLQKMLLRTRPYERTPGEAEKVYYHFLARMDRALVHDESLTELGREAARAFRQL
                     RAEGPDRPLVGLVGEIYLRHNRFSNNHLIGKLEQQGIEVRLATFTEWPLYTSLTFRRD
                     SWLERDWRNTLKGTLQVISQTRMEHRILAAMSEEFDLRHDYPVERVMKLASRYLSIDF
                     KGEAILSIGKAIEMVQEGAAGIVNAMPFNCMPGTIVSSLSRRVAEDLGQVPWLNISYE
                     GLRDSGEETRLEAFAEQVRTFARASGGAAERAPIESRPNGSA"
BASE COUNT          353 a          674 c          608 g          372 t
ORIGIN      
        1 cgaacttgcg cgccacttcg gtattcgaat ccacgtcgat cttggcgacc ttcacccggt
       61 cggcatactc ccgggcgact tcctcgagga tgggcgcgat cattttgcac ggtccgcacc
      121 aggtcgccca gaagtcgacg atcaccggct tgtcggcctg aatcacttcc tgctcgaatg
      181 aatcatcggt caccgtaatc ggcttgctca tgactgtagt cctccgtatc tgtcaggcgt
      241 caccacgttg tctgctgtaa cacatcttcg cgccggcggt tccttcggcg cttccggcgc
      301 aagacacaat gatatcgccc gtgtctcggc ccgccaagcg aaatccgccg gcctaagcgg
      361 agccgttcgg tcggctttcg atcggcgctc gctcggccgc gcccccgctt gcccgcgcga
      421 acgtccgcac ctgttcggca aacgcctcga ggcgcgtctc ctcccccgag tcgcgaagtc
      481 cctcgtagct gatattcagc caggggacct gcccgagatc ctcggcgacc cggcgcgaca
      541 gcgacgacac gatggtgccg ggcatgcagt tgaacggcat ggcgttcacg atcccggccg
      601 ccccttcctg caccatctcg atcgccttgc cgatcgagag gatcgcctcc cccttgaaat
      661 cgatcgacag gtagcggctt gccagtttca tcacgcgctc gaccgggtag tcgtgccgca
      721 ggtcaaactc ttcgctcatg gccgccagaa ttctgtgctc catccgcgtc tgcgatatca
      781 cctggagcgt tcccttgagc gtgttcctcc agtcgcgctc cagccacgag tcgcggcgga
      841 acgtcaggga ggtgtagagg ggccattcgg tgaaagtagc cagccgcacc tcaatcccct
      901 gctgttcgag ttttccgatg aggtggttgt tcgaaaagcg attgtgccgg agatagatct
      961 cgccgaccag gcccacgagc ggccggtccg gtccctccgc ccgcaattgt cggaacgccc
     1021 gcgccgcctc ccgtccgagc tccgtgagcg attcgtcgtg caccagcgcg cgatccatgc
     1081 gcgcgagaaa atggtagtac accttctccg cctcccccgg cgtgcgctcg tacggccgcg
     1141 tccgcagcag cattttctgg aggccgtcca cgaacaccag cccccgccag gcggtcttcc
     1201 ggaaatccgt cccctgcagc ccgaacccgt cttcgtatcc atccttcgaa ttgggcgaat
     1261 agatcggcac gtcggtgaac ccgagatcat cgagcaccat gcggtgaaag ggcgagtaca
     1321 gaccgaaccg gcacggtccg tcggcccccg gcatgaagaa aaccgagcgc ttgcggtcga
     1381 aatccggccg ccgcacgcgc cggatgaact ccccggtggt cacgacgcag gggaagcact
     1441 ccttgcccga ggtgaacttc ttgccgtact cgagcgtcaa ctcgtccggc tcgtcgagga
     1501 cttcagccgt cagcccgtag cgcgcgaagg ccgcccggag ggcgtaggcg tggtcgcaca
     1561 tgttcgggat atagatcgtc ttttcgaagg ggcggaattc gtcgcgcccc acactgaaac
     1621 tgccgaccgg tggcgtgtac cggtagaacc gaagggagtc gacgaaggcc tcgcaccgcg
     1681 tcaccagtcc tgcatccgcc gagtgctcgt ccaattccag ttgaaggtac ggcttccccg
     1741 ccatctgctt gcggaagaag tgggtgatga aggagtccgg tccgcacccg aacgaggtga
     1801 tgtagacggc aaagagaccg gggtgtttcc ggatgaagtc ggccgcctgg aggatccgcc
     1861 ggccgtagcg ccagtacata taggggaact cggaatccgc ctgctccgcc gggaggaagt
     1921 cgatcgggat cgcccggtag ccgaggttgc ggagcttgtc cggcaggtcc agcgacagcc
     1981 gccggtcgaa cccgttgtag gggcggc
//
LOCUS       DAOOKS010000392         1982 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_72229_length_1982_cov_2.933576, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000392 DAOOKS010000000
VERSION     DAOOKS010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72229_length_1982_cov_2.933576"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="PK112_06705"
     CDS             <1..672
                     /locus_tag="PK112_06705"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HPC11775.1"
                     /translation="AQWTNLISVLMGDYLFAKAFRIMVDSRSMDLMQAIAHATGRVSV
                     GELRQIEETGNYALSEEEYLTIIADKTASLFSLACETGPILAGRGRRDRQRFAHFGEK
                     IGTAFQIVDDLLDFVGDAEITGKEPGNDVLTGKVTLPLIHALKKAGRGGHDEIITHLR
                     RRDRRPSFERVLAFVREQGGIEHAYGRARALSEEGLAAITPVTPSEYFDCLVSLVQFT
                     ISRAS"
     gene            749..>1982
                     /locus_tag="PK112_06710"
     CDS             749..>1982
                     /locus_tag="PK112_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11776.1"
                     /translation="MFGFHDLSINVLTNSPVIVALALAALVGLAVHLYRRTNPPLPAY
                     LRAILGGLRIGALLLVFLALLEPVVSYTREYVRRPRVAVLVDQSLSMNKTEDGRSRRA
                     RTDSLLASATLDNLRRVADLAPFFFGGSLGAARGSVDSTSTALGDALFELDKAQTAEP
                     SDYWLLLSDGRSNAGRKPLDVAPSLRSPVISVDVSGGAESFDAGIDEVAFNPVLFVGR
                     PTEIRVKLTWRNAEGRTLDVRLVDSARTAAESRLTVTQAAGRGEVMLRYTPEAPGQRL
                     LQIALPPGENEESAANNQRTISVKVLKSRLSVLLAADRPDYEVGFLKRLLDGSDRYEV
                     TLLATGARAGNLAGRFPTVQTELNRYNLVILYDPDPAAYTSRQEIIRSYLREKGGAVW
                     LFMGPQFAARGPTSWLNDL"
BASE COUNT          308 a          698 c          666 g          310 t
ORIGIN      
        1 gcccagtgga ccaacctgat ttccgtcctc atgggcgact acctgttcgc gaaggcgttc
       61 cggatcatgg tcgactcccg gtcgatggac ctcatgcagg cgatcgccca cgccaccgga
      121 cgcgtctcgg tgggcgagct gcgccagatc gaagaaacgg gcaactacgc gctgtcggag
      181 gaggagtacc tgaccatcat cgccgacaag accgcctcgc tgttcagcct cgcctgcgag
      241 accggcccga ttttggccgg gcgcggcagg cgcgaccgcc agcgcttcgc ccacttcggc
      301 gagaagatcg gcaccgcctt ccagatcgtc gacgacctcc tggatttcgt gggcgatgcg
      361 gaaatcacgg ggaaggagcc ggggaacgac gtgctcacgg ggaaggtgac gctgccgctc
      421 atccatgccc tgaagaaggc gggccggggc ggccacgacg agatcatcac ccacctgcgg
      481 cggcgcgacc ggcggccctc gttcgagagg gtgctggcct tcgtgcgcga gcagggcggc
      541 attgagcatg cctacgggcg ggccagggcg ctgtcggagg aggggctggc ggcgatcacg
      601 ccggtgacgc cctcggaata cttcgattgc ctcgtgagcc tggtgcagtt caccatttcg
      661 cgggcctcct gagggcggcc gggaaagccc gccccgcgcg cgccggccgc gacaacccgc
      721 gccccccgac cggggtataa aaagcagtat gtttggcttc cacgacctct ccataaacgt
      781 cctgaccaac tccccagtga tcgtggccct cgcgctggcc gcccttgtcg ggctggccgt
      841 ccacctctac cggcggacca atccgcccct gccggcatac ctgcgggcga tcttgggcgg
      901 actgcggatc ggcgcgctcc tcctggtctt cctggccctc ctggagccgg tcgtcagcta
      961 cacccgggaa tacgtgcgcc ggccgcgcgt ggccgtcctg gttgaccagt cgctctcgat
     1021 gaacaagacg gaggacgggc gcagccgccg cgcccggacc gattcgctgc tggcgagcgc
     1081 gactctcgac aacctccgcc gggtcgctga cctcgctccc ttcttcttcg gcggcagcct
     1141 cggcgccgcc cgcgggtcgg tcgacagcac gagcacggcg ctcggcgatg ccctcttcga
     1201 actcgacaag gcccagaccg ccgaacccag cgactactgg ctgctgctgt cggacggccg
     1261 gtcgaacgcc gggcgcaagc cgctcgacgt ggcgccgtcg ctccggtccc ctgtgatcag
     1321 cgtcgacgtc tccggcggcg cggagagttt cgacgccggg atcgatgaag ttgcgttcaa
     1381 cccggtgctg tttgtcggcc ggccgaccga gatccgggtg aaactgacct ggcgcaacgc
     1441 cgaggggagg acgctcgacg tgcgcttggt cgactcggcg cgcacggcgg ccgagagccg
     1501 cctgaccgtc acccaggcgg ccgggcgggg agaggtgatg ctgcggtaca cgcccgaagc
     1561 ccccggccag cgcctcctgc agattgccct tccgccgggg gagaacgagg agtcggcggc
     1621 caacaaccag cggaccattt cggtgaaagt cctcaagagc cgcttgagcg tgctgctcgc
     1681 ggccgaccgg cccgactacg aggtcggctt tctcaagcgg ctgctcgacg gatccgaccg
     1741 ttacgaggta acgctgctgg ccacgggggc gcgcgccggc aacctcgccg gccgcttccc
     1801 gaccgtgcag accgaactca accgctataa tctggtgatc ctgtacgatc ccgatcctgc
     1861 ggcctacact tcccgccagg aaatcatccg gtcgtacctg cgcgagaagg gcggggccgt
     1921 ctggctgttc atggggccgc agttcgccgc ccgcgggccg acctcgtggc tgaacgatct
     1981 gc
//
LOCUS       DAOOKS010000393         1981 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_72281_length_1981_cov_2.471963, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000393 DAOOKS010000000
VERSION     DAOOKS010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72281_length_1981_cov_2.471963"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1163)
                     /locus_tag="PK112_06715"
     CDS             complement(<1..1163)
                     /locus_tag="PK112_06715"
                     /inference="COORDINATES: protein motif:HMM:NF037626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11777.1"
                     /translation="MTTGTDRSHTTHSRLRQVIGRWLEYAPARELAARLEEGPAEPVP
                     IAGLAGSAPAFLLRALTDTLGRPVLAVTVTSEQAADLADDLAVLLGPDRVGHYPARQV
                     LPYDFKAPQGEVMGRRISSLADLMDNRLAAVVAPLRAVLEPTIARDHLEKSRVRLEVG
                     RAADLDDLVRRLVALGFRRVPVVEEVGDFALRGGLVDFFSTGSTAPSRVEFFGDDVDT
                     IRLFDVGSQRTIGCVDWVNLLPRREVPITMETVEQYLAALPEHDADYIRDRYLNDPEL
                     PGLEWLAILFGVRPGSLREYLPPDTVVWLEGEHLLRDEAEGILAEGRGLYQRLKGRLE
                     KLPAPEAYYHSVEGAFAWLAAHPRIDHAPFRGGRRDVIDFGCRPHPSLGSRLDL"
     gene            complement(1307..>1981)
                     /locus_tag="PK112_06720"
     CDS             complement(1307..>1981)
                     /locus_tag="PK112_06720"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPC11778.1"
                     /translation="DPGKYLEQGGAGVLKGSRSAITEKDTPSGMLKNESDKSGGGPSE
                     MEKKQMKEAALKIISQLEQQEAFHRLRDNVKMQLTSEGLRIILNESAESPAFFEPGSA
                     KLLRQSAVILMTIAGELGHLQNRIVLEGHTDAAYTGDLLYTNWELSADRANAARELLE
                     AHGLWDGQVREVRGFAEQFPMIADNPADARNRRVTIVVLYEHASMAYDQMEVGADLMA
                     LAEAQE"
BASE COUNT          296 a          646 c          718 g          321 t
ORIGIN      
        1 aggtcaaggc gcgagcccag cgaggggtgc ggcctgcaac cgaagtcgat gacgtcgcgg
       61 cggccgccgc ggaaaggggc gtggtcgatg cgggggtggg cggccagcca ggcaaaggcg
      121 ccctcgaccg agtggtagta ggcctcgggc gcgggaagct tctccagccg ccccttgagc
      181 cgctggtaga gaccccgccc ctcggcgagg atcccctccg cctcgtcgcg gagcaagtgc
      241 tccccttcga gccagacgac ggtgtccggc ggcaggtact cgcggagcga cccggggcgg
      301 acaccgaaca ggatcgccag ccactcgagc cccggcagct cggggtcgtt gaggtagcgg
      361 tcgcggatgt agtcggcatc gtgctccggc agcgccgcga ggtattgctc gactgtctcc
      421 atggtgatcg gcacctcccg gcgcgggagg aggttgaccc agtcgacgca gccgatggtg
      481 cgctgggagc cgacgtcgaa gagacggatg gtgtcgacgt cgtcgccgaa gaattcgacg
      541 cgcgagggag cggtcgagcc ggtggagaag aagtcgacca gcccgccgcg gagggcgaag
      601 tcgccgactt cctcgaccac cgggacgcgc cggaaaccga gcgcgaccag gcggcgcacg
      661 aggtcatcga ggtcggccgc ccgtccgact tcgagccgga cccggctttt ttcgagatgg
      721 tcgcgggcga tcgtcggttc gagcacggcc cgcagcgggg cgaccacggc cgccagccgg
      781 ttgtccatga ggtcggccag cgacgaaatc cgcctcccca tgacctcccc ctgcggcgcc
      841 ttgaagtcgt acgggaggac ctggcgggcg gggtagtggc cgacgcggtc cgggccgagc
      901 agcaccgcca gatcgtccgc cagatcggcc gcctgctcgg aggtgacggt caccgccagc
      961 accggtcggc cgagcgtatc ggtcagggcg cgaaggagga aagcgggcgc cgagccggcc
     1021 agcccggcga tcggaaccgg ttcggccggg ccctcttcaa ggcgggcggc cagttcccgc
     1081 gccggggcgt attcgagcca ccggccgatg acctgccgga ggcggctgtg ggtggtgtgc
     1141 gaacgatccg tgccggtggt catgcgctgt agctgctttc gttcccgcgg gcggtcaggc
     1201 ccgcgccaaa cacaaaaacc cctggccgtc gccgaccggg gagggtggtt cactcgtcgc
     1261 tggtattata caccgccggc ggggcggatg ccagtttttt gcgcgctcac tcctgcgctt
     1321 cggccagcgc catcaggtcg gcgcccactt ccatctgatc gtacgccatt gaggcgtgtt
     1381 catagagcac gacgatcgtc acgcggcggt tgcgggcgtc ggccgggttg tcggcgatca
     1441 tcgggaactg ctcggcgaac ccgcgcacct cgcgcacctg gccgtcccac agcccgtgcg
     1501 cttccagcag ttcgcgggcg gcgttggccc ggtcggccga cagctcccag ttggtataga
     1561 ggaggtcgcc ggtgtaggcg gcgtcggtgt gcccttcgag cacaatccgg ttctgaaggt
     1621 gcccgagctc cccggcgatc gtcatgagaa tcaccgcgct ctggcgcagg agtttggccg
     1681 agccgggttc gaagaacgcc ggagactcgg ccgactcatt gaggatgatc cgcagcccct
     1741 cggaggtcaa ctgcatcttg acgttgtcgc ggaggcggtg gaaagcctcc tgctgctcaa
     1801 gctggctgat gatcttgagg gcggcctctt tcatctgctt cttctccatt tcggaggggc
     1861 cgccgccgga tttgtcgctt tcgttcttga gcatcccgga gggggtatct ttctcggtga
     1921 tggcggagcg cgagcctttc aggacgccgg cgcccccctg ctccaggtat ttgccgggat
     1981 c
//
LOCUS       DAOOKS010000394         1977 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_72484_length_1977_cov_2.685224, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000394 DAOOKS010000000
VERSION     DAOOKS010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72484_length_1977_cov_2.685224"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(42..>1977)
                     /locus_tag="PK112_06725"
     CDS             complement(42..>1977)
                     /locus_tag="PK112_06725"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HPC11779.1"
                     /translation="TAISDPSPSTDTLVEMGDVGDSTAAVDDELWRQLDLAEEYHAMG
                     VIANREGSWEEAQYYFEQGLTILASLDIEGDSLETPEAAAYSILLDDVIADYRVTLLS
                     LGDLQGDVSPAVLIERFGDLANNLSNDSMLVFTTEPEEITFDIPVYMNERVRKSVVYF
                     QTVAREAFEKFLRRSKKYDRMMKDIIRQYEMPEDLVYLSMVESGFNPRAYSWARASGL
                     WQFIASTGRLYGLDRDWWIDERRDPIKSTHAACRFLKDLYQKFGSWELAMAAYNGGPG
                     RVERTIAKQGTRDFWKLNLRRQTMDYVPLIMAAAMISKEPEKYGFESIEFEDELTWDE
                     VSIDRCLDLTVVADAVGCTVGELEDLNPELLRKFTPPNVKDYRLKIPSGSRTKFLAAY
                     ESMESPKESSWVRHTIRRGETVSSIASRYGVSQYSILAANNMSSRSKIYAGRTLIVPV
                     PLDGAPSGGGDGRSYESRNGVYVVRSGDTMWDIAKAFGTSVDALRRSNAIGRGARIYV
                     GQKLRIPGGSHPADAGRTYAGTPAASKKSSGSSQRYTVRSGDTLWEIARKFGTTTSNI
                     RRLNNLGRSSRIYPGQVLTVGGGSGAAADYVIHRVRRGESLSQIAQKYRTTIAKIIET
                     NALSNPDQLQVGDQLRIYTE"
BASE COUNT          326 a          669 c          605 g          377 t
ORIGIN      
        1 ccgaccacta cgtcgcgcgt ccgcgccata cccgacgtcc tttactccgt gtagatcctc
       61 agctggtccc cgacctgaag ctggtcgggg ttactcagcg cattcgtttc gataatcttc
      121 gcgatcgtgg tccggtactt ctgggcaatc tggctgagcg attcgccgcg ccggacgcgg
      181 tgaatcacgt agtcggcggc ggcgccggaa ccaccgccca cggtgaggac ctgcccgggg
      241 taaatgcggc tggagcgccc gaggttgttc aggcggcgga tgttcgaggt cgttgtccca
      301 aacttccgcg cgatctccca cagcgtatcg ccggagcgga ccgtgtatct ctgcgaactg
      361 ccggaggact tcttcgaagc cgccggcgtt ccggcgtagg tccgacccgc gtcggccgga
      421 tgggatccgc cggggattct gagtttctgg cccacataga tgcgcgcccc gcggccgatg
      481 gcgttgctcc gccgcagcgc atccacggac gtcccgaagg ctttcgcaat atcccacatg
      541 gtgtcgcccg agcgcaccac gtagacgccg ttgcgcgatt cgtaggacct tccgtcgccc
      601 ccgcccgacg gcgcgccgtc aagcggcacg ggcacgatca gcgtcctgcc cgcgtagatc
      661 ttcgagcggc tgctcatgtt gttcgcggcc aggatggagt actgcgacac cccgtagcgg
      721 gaggcgatgg agctgaccgt ctccccccgg cgaatggtgt gccgcaccca gctcgactcc
      781 ttgggcgatt ccatgctctc gtaggcggcc aggaatttcg tccgcgaccc ggaagggatc
      841 ttcagccggt agtccttcac gttcgggggc gtaaacttgc gcagcagttc cggattgagg
      901 tcttccagct cgcccaccgt gcagccgact gcgtccgcca ccaccgtgag atcgaggcac
      961 cggtcgatcg aaacttcgtc ccaggtgagc tcgtcttcga actcgatact ctcaaaaccg
     1021 tacttctcgg gttctttgga gatcatcgcc gccgccatga tgagcgggac gtagtccatc
     1081 gtctggcgcc gcaggttgag tttccagaaa tcccgcgtcc cctgcttggc gatcgtccgc
     1141 tccacccggc cgggaccgcc gttgtaggcc gccatggcca gttcccacga cccgaatttc
     1201 tggtagagat ccttcaggaa acggcaggcg gcgtgggtcg acttgatcgg gtcgcgccgc
     1261 tcgtcgatcc accagtcgcg gtccaacccg tagaggcgcc cggtggaggc gatgaactgc
     1321 cacagtcccg aggcccgggc ccacgagtag gcgcgcggat taaaccccga ctcgaccatc
     1381 gagaggtaca cgagatcctc cggcatctcg tactgccgga tgatgtcctt catcatccgg
     1441 tcatatttct tcgaccgccg cagaaacttc tcgaacgcct cccgcgccac ggtctggaaa
     1501 tacaccaccg acttgcgcac ccgctcgttc atgtagacgg ggatatcgaa agtgatctcc
     1561 tccggctcgg tcgtgaacac cagcatggag tcgttgctga gattgttcgc aagatcgccg
     1621 aaccgctcga tcagcacggc gggcgagacg tccccctgca gatcgccgag cgacagaagg
     1681 gtgacccggt agtcggcgat gacgtcgtcc aggaggatgc tgtaggccgc cgcctcgggc
     1741 gtctccagcg agtcgccctc gatgtcgagc gacgccagga tggtcaggcc ctgctcgaaa
     1801 tagtactggg cctcctccca gcttccctcg cggttggcga tcacgcccat cgcgtggtac
     1861 tcttcggcca ggtcgagctg ccgccacagc tcgtcatcca cggccgctgt cgaatccccc
     1921 acgtccccca tttccacgag cgtgtcggtc gagggcgagg ggtcgctgat cgcggtc
//
LOCUS       DAOOKS010000395         1965 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_73155_length_1965_cov_4.528796, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000395 DAOOKS010000000
VERSION     DAOOKS010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73155_length_1965_cov_4.528796"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="PK112_06730"
     CDS             complement(<1..277)
                     /locus_tag="PK112_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11780.1"
                     /translation="MFTPTVLGIDIGSVTVKTVRLDDGGAVRKRYERFRGGPFETLQR
                     ILREDHADLVGRPVALGLTGIGGKTAQGILGGKFFGEIASIAAGNFHV"
     gene            399..851
                     /gene="dtd"
                     /locus_tag="PK112_06735"
     CDS             399..851
                     /gene="dtd"
                     /locus_tag="PK112_06735"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013353029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HPC11781.1"
                     /translation="MRVVLQRTTGCEVRIGGRLHAAAGPGLLLLFGTVSGDAEASCAW
                     LADKIVNLRIFEDEAGKMNRSALEVGAEMMVVSQFTLYADTRRGRRPSFDGAMAPDRA
                     EQLYDRFVELLRDTGLTVATGVFGAHMDVSLTNHGPVTFILEHGVPAA"
     gene            832..1446
                     /locus_tag="PK112_06740"
     CDS             832..1446
                     /locus_tag="PK112_06740"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HPC11782.1"
                     /translation="MAYPQLERLARRRRLVLGSRSPRRLTLLRGMGVAFDQLIPEVEE
                     RRLPGEPPLAYACRLAEDKARWLMPRLAPDQVGLTCDTIVILGDDVLEKPSDSAEALA
                     ILSALSGTRHVVSTAIAWTADRRLLASGYETTEVYFRPVTAAQLRDYIATGEPMDKAG
                     AYGIQGMGAFLVDRIDGNLDTVIGLPRTLVEQLAGEAISQLEHL"
     gene            1487..>1965
                     /locus_tag="PK112_06745"
     CDS             1487..>1965
                     /locus_tag="PK112_06745"
                     /inference="COORDINATES: protein motif:HMM:NF024805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPC11783.1"
                     /translation="MLKLERERQGMRLEDLAASLRISEANLRALEAGNPAALPSPVYF
                     NLFAKTYAESLGIDYAATMDAIKADMTEIEVPEPRKAKKGKAQPAPQAAPPPPPSAAE
                     SFAESAAAEPAAAGRKGRTLVVIGSVAAALVILAGGYFAVTQLLRADSTQPVDASEEA
                     "
BASE COUNT          321 a          669 c          637 g          338 t
ORIGIN      
        1 agacatggaa attgcccgcg gcgatcgagg cgatctcgcc gaagaatttc ccgcccagaa
       61 tcccctgcgc ggtcttgccg ccaatgcccg tcagccccag cgccaccggg cgtcccacca
      121 ggtcggcgtg gtcttcccga aggatgcgct gcagggtttc gaacggtccg ccgcggaatc
      181 gctcgtagcg cttgcggacc gcccccccat cgtccagtcg aacagtcttc accgtgaccg
      241 agccgatgtc gatgccaagt actgtcggag tgaacatgcc ggcaaagaac ggcggcggcg
      301 cgcacggggc aagtcttttt gcgcgggccg gcgcgccgtt tcccgttgtc tttttgccgc
      361 cgccgcctat attcagggac ttaagtggag actgtccgat gagagttgtc ctccagcgca
      421 cgaccggctg cgaagtccgg atcggcggcc gccttcatgc cgccgcgggt ccgggcctgc
      481 tgctcttgtt cggcaccgtc agcggcgacg ccgaggcttc gtgcgcctgg ctcgcggaca
      541 agatcgtcaa cctgcgcatt tttgaggacg aggccggcaa gatgaaccgc tcggcgctgg
      601 aggtgggcgc cgagatgatg gtcgtttcgc agttcacgct ctacgccgat acccgccgcg
      661 gccgccggcc ctcgttcgac ggcgcgatgg cgcccgaccg ggcggagcag ttgtacgacc
      721 gtttcgtgga acttctccgg gacaccggcc tcaccgtcgc cacgggcgtg ttcggcgccc
      781 acatggacgt cagcctcacc aaccatggcc cggttacatt tatcctcgaa catggcgtac
      841 ccgcagcttg agcggctcgc gcgccgccgc cggctcgtcc tggggtccag gtcgccgcgg
      901 cgcctcaccc tgcttcgcgg catgggcgtg gcattcgacc agttgatccc ggaggtcgag
      961 gagcgccgcc tccccggcga gcctccgctt gcctacgcct gccgactggc cgaggacaag
     1021 gcgcgctggc tcatgccccg cctggccccg gatcaggtcg ggctcacctg cgacacgatc
     1081 gtcattctgg gcgacgacgt cctggagaaa ccgtccgaca gcgccgaggc gctggccatt
     1141 ctctccgccc tctcgggcac gcgccacgtg gtctccacgg cgatcgcctg gaccgccgac
     1201 cgccgattgc tcgcctccgg ctacgagacc accgaggtgt atttccggcc ggtcaccgcc
     1261 gcccagcttc gcgactacat cgccaccggc gagccgatgg acaaagcggg ggcctacggc
     1321 attcagggaa tgggggcgtt tttagttgac agaattgatg ggaatctgga tactgtcata
     1381 ggtctccctc gaacgctcgt cgagcagctc gccggagaag cgatcagtca gttggaacat
     1441 ctgtaagata ctgcacatgg gtgagttaga gacaaaattt ggagaattgc tcaagctgga
     1501 gcgggaacgc caggggatgc ggcttgagga tctcgccgcc agcctccgga tttccgaggc
     1561 caacttgcgg gcgctggagg cgggcaaccc ggccgccctt ccctcgccgg tctatttcaa
     1621 tctgtttgcc aagacctacg ccgagtctct cggtatcgac tatgcggcca ccatggatgc
     1681 catcaaagcc gacatgactg agatagaggt tccggaaccg cggaaggcga agaaaggcaa
     1741 ggcgcagccg gcgccgcaag cggccccgcc gccccctccg tcggccgccg aatcgttcgc
     1801 cgagtcggcg gcggccgaac cggccgccgc gggcaggaag ggccgcacgc tggtggtcat
     1861 cgggtcggtc gcggccgctc tcgtcatctt ggcgggcgga tacttcgcgg tcacccagtt
     1921 gctccgcgcc gacagcacgc agccggtcga cgcaagcgag gaggc
//
LOCUS       DAOOKS010000396         1965 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_73170_length_1965_cov_3.379581, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000396 DAOOKS010000000
VERSION     DAOOKS010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73170_length_1965_cov_3.379581"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="PK112_06750"
     CDS             <1..586
                     /locus_tag="PK112_06750"
                     /inference="COORDINATES: protein motif:HMM:NF013038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HPC11784.1"
                     /translation="LARLRRLGISPRLRHTASSAATILFEKTRYDLVRPGISVYGHWP
                     SKETYLSYRLQGGGNRLFEPVLTWKTRITQLKKVAADSFVGYGCTYRTTAPTRLAVLP
                     IGYYDGYDRSLSNRAYVLVHGRRAPVRGRICMNLMMVDVTDISGVQLHDEVVLIGRAG
                     EERVSAEQIGDWAGTINYEVLARLSLSTPRLIVD"
     gene            792..1466
                     /locus_tag="PK112_06755"
     CDS             792..1466
                     /locus_tag="PK112_06755"
                     /inference="COORDINATES: protein motif:HMM:NF003992.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HPC11785.1"
                     /translation="MNVSSKTGLKKKISESTIHRLSLYCRALSLLEKENYETVSSKEL
                     AKREKLTPAQVRKDLSFFGSFGTRGLGYPVNELKQRIASILGIDRVWRVALVGVGNIG
                     SALVSYKEFMRQGFHIVKLFDNDQRKIGSNHKGIIVSDIANLEKELKEAGIEIVVLAV
                     PATVAQYIVDDVVRAGVRAVLNFAPVNLRVPPDVYLRNENMSMELEYLSFALVNNPPK
                     KASKQG"
     gene            1655..>1965
                     /locus_tag="PK112_06760"
     CDS             1655..>1965
                     /locus_tag="PK112_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11786.1"
                     /translation="MRRSLRRLLRTALPPGIVLGALVSATGGWSQESAVWGDHALGTR
                     SYDDYATPDYCGTSCHVDFYRQWEQAMMAQAYVHHWDEIEYFKLAVPHAEKDAKVAGV
                     KA"
BASE COUNT          392 a          515 c          699 g          359 t
ORIGIN      
        1 gctggcgcgg ttgcggcggc tcgggatcag cccgcgcctc cggcacacgg cctcgtcggc
       61 ggcaaccatc ctctttgaga aaacccgcta cgacctggta cgaccgggga tatcggtcta
      121 cggccactgg ccatcgaagg aaacctacct ctcctaccgc ctccaggggg gcgggaaccg
      181 tctcttcgag ccggtcctca cctggaagac gcggatcacg cagttgaaga aggtggcggc
      241 ggacagcttt gtcggatacg gctgcaccta tcggacgacg gcgccgacgc ggctggcggt
      301 gctgccgatt gggtactacg acggatatga tcggagcctg tcgaaccggg cgtacgtgct
      361 ggtgcacggg cggcgggcgc cggtgcgggg gcgcatctgc atgaacctga tgatggtgga
      421 tgtcacggat ataagcgggg tgcagctgca cgacgaggtc gtgctgatcg gtcgggcggg
      481 cgaggagcgg gtgtcggccg aacagatcgg agactgggcg gggacgatca attacgaggt
      541 tctggcgcgc ctgtcactgt ctactccacg tctcatagtg gattaggcgg gtgagccgcc
      601 ttcaggaccg ggggcattgc tgggggtctt tgtgataacg aaacctatta ccaagttgta
      661 agatacacgg ttaaatctgc ggtgcgggcc agagaaaagt gttgacagcc gattgggccg
      721 atgctataat ctcttgtgat tcatttcaca tagattgtga acgactgtaa cttacagcgg
      781 cgagtgcaca gatgaatgtc tccagcaaga ccggtctcaa gaagaagatt tcggaatcaa
      841 caatccatcg gctgtcgctc tactgccggg cgctgagcct cctggagaag gagaactacg
      901 agaccgtttc gtccaaggag ctggcgaagc gggagaagct gacgccggcg caagtgcgca
      961 aggacctgtc gttcttcggg tcgttcggga cgcgggggct cgggtatccg gtgaatgagc
     1021 tgaagcagcg gattgcatcg attctgggga ttgaccgggt ctggcgggtg gccctggtcg
     1081 gggtggggaa tatcgggtcg gcgctggtca gttacaagga gttcatgagg cagggattcc
     1141 acatagtcaa actgttcgac aacgaccagc ggaagatcgg ttcgaaccac aagggaatca
     1201 tcgtttcgga tatcgccaat ctcgagaaag agctgaaaga ggcgggcatc gagatcgtgg
     1261 tgctggcggt gccggcgacg gtggcgcagt acattgtgga cgacgtggtg cgggcggggg
     1321 tgcgggcggt cttgaatttc gccccggtca atctgcgggt gccgccggat gtctacctgc
     1381 gcaacgagaa catgtcgatg gaactggagt acctctcgtt tgcgctggtg aacaacccgc
     1441 cgaagaaggc gagcaagcag ggctgattcc ggggggcagc ggggatgttc gccggccacg
     1501 cagcggttgt cactttcccc cgctctctgc gccgcacgcg gttcgcgcgg attcactgat
     1561 cacagggatc ggccggcccg gaactgaaaa atcaggcggg accaccaaca ataccgatac
     1621 tcatcacccg aagcggggag gaaaaggagg ctacatgagg cgatcgttac gtcggctgct
     1681 acggacggcc ctgccgccgg gaatcgtgct cggcgccctg gtgtcggcga cgggcgggtg
     1741 gagccaggag tcggcggtgt ggggggacca cgcgctgggg acgcggagct acgatgacta
     1801 cgcgacaccg gactactgcg gcacttcctg ccacgtcgac ttctatcgcc agtgggagca
     1861 ggcgatgatg gcgcaggcgt atgtgcacca ctgggacgaa atcgagtatt tcaagctggc
     1921 ggtgccgcac gcggagaagg atgcgaaggt ggcgggggtg aaggc
//
LOCUS       DAOOKS010000397         1963 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_73295_length_1963_cov_3.399895, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000397 DAOOKS010000000
VERSION     DAOOKS010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73295_length_1963_cov_3.399895"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            183..1151
                     /locus_tag="PK112_06765"
     CDS             183..1151
                     /locus_tag="PK112_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HPC11787.1"
                     /translation="MPDSAPVERPIKVGISTCLLGQKVRFDAGHKHDRYITDVLGGYF
                     RFVPVCPELEVGMGVPRESVRLEGAPDAPRLVGTRSGADWTERMNRYARERTARPDLA
                     DISGYILKKDSPSCGLERVKVYPPSGMAERKGTGLFAAALRQRFPLLPIEEEGRLNDH
                     RLRENFIVRVFAYDRLQTLFRERFSRGRLVAFHTAHKYLLMAHSPKHYAELGRLVAAV
                     SRHSPAAMREQYRRLFMEALAVPTTTRKNVNVLQHLLGYLKHRLGPEEKQDILNVIAD
                     YHRELIPLVVPLTLLRHYVDKHRIAYIQDQVYLNPHPKELMLRNHV"
     gene            1676..>1963
                     /locus_tag="PK112_06770"
     CDS             1676..>1963
                     /locus_tag="PK112_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11788.1"
                     /translation="MIKRLTLLTLVALLVAPAFSNAKDMTGKYGLGYFTTDAPIGFRY
                     WATPKLGIDVGVGFKSDAAWVGEYDETEDRTVYSKETASSFWFEVGFSYVVV"
BASE COUNT          335 a          650 c          620 g          358 t
ORIGIN      
        1 ggcccggcgg acgtgattca cagccggcgg cgccgccggc gggcgttcct cgccgcttgc
       61 gttgcggctc tgcgcaagct accttggcgt gcgacccttg cctcccggag gcccgcagaa
      121 gcgcgccgtg ctccggacgg ttcgccggac ccgccggttc acatcgagat cagaggacgc
      181 ccatgcccga ttccgctcct gtcgaacgcc cgatcaaggt gggtatcagc acctgcctgc
      241 tcggccagaa ggtccgtttc gatgccgggc acaagcacga tcgctacatc acggacgtcc
      301 tcggcggcta ctttcgcttt gtcccggtgt gcccggagct ggaggtggga atgggcgtcc
      361 cgcgcgaatc ggtccggctg gagggggcgc ccgacgcccc gcgcctggtc ggtacccgga
      421 gcggcgccga ctggacggag cggatgaacc gctatgcgcg cgagcgcacc gcccggcccg
      481 atctcgccga catctccggc tacatcctca agaaggactc ccccagttgc gggctcgagc
      541 gggtgaaagt gtatcccccc tccggcatgg ccgagcgcaa agggaccggg ctgttcgccg
      601 ccgccctgcg gcagcggttc ccgctgctgc cgatcgagga ggagggccgc ctcaacgacc
      661 atcgcctgcg cgagaacttc atcgtccgcg ttttcgccta cgaccgcctg cagacgctgt
      721 tccgggagcg tttcagccgc gggcgtctgg tcgccttcca cacggcccac aagtatctcc
      781 tcatggcgca cagcccgaag cactatgcgg agctcggccg gctcgtcgcc gcggtgagcc
      841 ggcactcgcc cgccgccatg cgggagcagt accgccggct tttcatggaa gccctcgcgg
      901 tcccgaccac cacgcgcaag aacgtcaacg tcctccagca tctcctcggg tatctcaagc
      961 accggctcgg ccccgaggag aagcaggata tcctgaacgt catcgccgac tatcatcggg
     1021 agctgatccc gctggtggtc ccgctcacgc tgctgcgcca ctacgtcgac aaacatcgca
     1081 tcgcctacat ccaggaccaa gtgtatctga acccgcatcc gaaagagctc atgctccgga
     1141 accacgtgtg aggcatctct ccgcacgcta cctgcaagac cgcggcgccg attacgtacg
     1201 gtgcctgcgc gggcagctcg accgccgcat cgagctgacg gtcggcgcgg cgatcccgag
     1261 gtcccctcgg gcggcgacgc cgacctcgaa atccatcctg cggatcatct gcccgcatcg
     1321 gcgcagattg cggagccggc ggcgatgctg aaggcggcgg ccgatgggcg gccgataccc
     1381 aatccggtgg atccccggag gggctattag ggggcgacga cgcttccgcg gcaacactag
     1441 gaaatttttt cccacaaccg cagtggcgcg tggatggtcg atgcggggac tatgagggtc
     1501 gatcttggca ggtgcgggtg gggccgtatt ttcgacgttg ttgttttttc cgccaggcgg
     1561 cgtttttttt ggtgacaacc acttgtgcct gtctgtaatt tggccccgta ttgaccaaca
     1621 acgcggagag aaaacggaaa ctggactctc tgccaacgta tcaaggaggt gctttgtgat
     1681 caaacggtta acgcttctca ccctggtcgc gctgctggtc gcgccggcgt tctccaatgc
     1741 caaggacatg accggcaagt acggcctcgg ctatttcaca actgacgccc caatcggctt
     1801 ccggtattgg gccactccca aactgggtat cgatgtcgga gtagggttca agtccgatgc
     1861 ggcttgggtc ggcgagtacg acgaaaccga agacagaacg gtgtactcca aggagacggc
     1921 tagcagcttc tggttcgagg ttggattctc gtatgtggtc gtt
//
LOCUS       DAOOKS010000398         1960 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_73448_length_1960_cov_3.940157, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000398 DAOOKS010000000
VERSION     DAOOKS010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73448_length_1960_cov_3.940157"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            270..800
                     /locus_tag="PK112_06775"
     CDS             270..800
                     /locus_tag="PK112_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HPC11789.1"
                     /translation="MLIFATIFAVGFLTLLLSVIFGHDTDFHVDADAGGVGADGHGPG
                     VINIKIIALLLVGFGAVGFGFRATAGWTMMKSSMAGLVGAAAVGAIGYLILRLFYASQ
                     ASSTIGDEEIIGCEARLIDAIAGAEYGQAACVIRGREITFLARSADGRSIARNAPVRI
                     VGKSGGVVTVAPTQNG"
     gene            835..>1960
                     /locus_tag="PK112_06780"
     CDS             835..>1960
                     /locus_tag="PK112_06780"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HPC11790.1"
                     /translation="MSEMIQFFVWPIIGLAIVIALFVLVKALSLFYVRVPPNKAAFFY
                     GARSGKKGLQRATQAEVPGVPGVTVLPPGTVVVTGGGRIRKPIIEDVQFLDLTEITLP
                     QIRVENMPNTDGVLVTVEAVANIRFKDDAQSLLAAGARFLSMPHEKVQSVARETLEAN
                     LRGVVGTLTVDQLIKDRDAFRQQVLREAAEDLARLGMQIDVFNPQSITDQQGYIEALG
                     KKRTAEVKRDAMIGEAEAQAEAKKRSTDAERAAEVVAQENERLKSEAQKNTNVAKQQY
                     AAEISKQTAITEQAGPLATAGERQKVIVAEVKIEEERSRAQIAVEEQNILREQKAQEA
                     EVVVPAKAKADSQVKEAEGYRQAQISRADGDKYATIARADA"
BASE COUNT          369 a          593 c          702 g          296 t
ORIGIN      
        1 aggagctggc caacatgttc gacatgaacc ggcggtacat caccgagcgg acagccgatc
       61 tcgaggagag ccggaagctc tatcccggga tccgggactt cgatgcgtgg gtgcgggaga
      121 accgggagtt cttcgcctcc cttctggaac cggcgcggtg acgcgccggc cgggcgccga
      181 taaatctttc gcctccgcac ggtgaatatc gtatacttgg ggcgagtata tcaaacggtc
      241 tcggtagaga agttgcggag gttgaggcca tgctcatctt tgccaccatt ttcgcagtgg
      301 ggttcctgac ccttctgctc agcgtcattt tcgggcacga tacggacttc cacgtcgacg
      361 ccgacgccgg cggggtcgga gccgacggac acgggccggg agtcatcaac atcaaaatca
      421 tcgccctcct cctggtcgga ttcggggcgg tcggattcgg gtttcgggcc acggcagggt
      481 ggacgatgat gaagtcgtcc atggcgggcc tggtgggggc ggcggcggtc ggcgccatcg
      541 gctacctcat tctccggctg ttctacgcca gccaggcgag ttcgacgatc ggggacgagg
      601 agatcatcgg gtgcgaggcg cgtctgatcg atgcgatcgc gggcgcggag tacgggcagg
      661 cggcgtgtgt catccgcggc cgggagatca ccttcctggc ccggtcggcg gacggccggt
      721 cgatcgcccg gaacgcaccg gtgcggatcg tgggcaagtc gggcggcgtc gtgaccgtcg
      781 cgcccaccca aaacggctag aatatcacca accacaggag cggaggtctc actcatgtcg
      841 gagatgatac agtttttcgt gtggccgatt atcggcctgg cgattgtcat cgccctcttc
      901 gtcctggtca aagcgctgtc gctgttctac gtgcgcgtcc ctccgaacaa ggcggccttc
      961 ttctacggcg cgcggagcgg caaaaagggg ctgcagcggg cgacgcaggc ggaggttccc
     1021 ggcgtgcccg gggtgaccgt gctcccgccc gggacggtcg tggtcacggg gggcgggcgg
     1081 atccgcaagc cgatcatcga ggatgtccag ttcctcgacc tgaccgaaat caccctgccg
     1141 cagatccggg tggagaacat gcccaacacc gacggggtgt tggtgacggt cgaggcggta
     1201 gcgaatatcc ggttcaagga cgacgcccag tcgctgttgg cggccggggc ccgcttcctc
     1261 agcatgccgc acgagaaggt gcagagcgtc gcccgcgaga ccctcgaggc gaacctgcgc
     1321 ggcgtggtcg gcacgctgac ggtcgaccag ttgatcaagg accgcgacgc gttccgccag
     1381 caggtgctgc gggaggcggc ggaggatctc gcccggctgg ggatgcagat cgacgtgttc
     1441 aacccgcagt cgatcaccga ccagcagggg tacatcgagg cgctcgggaa gaagcgcacg
     1501 gccgaggtca agcgcgacgc gatgatcggc gaggccgagg cccaggcgga ggcgaagaag
     1561 cgttcgaccg acgccgagcg ggccgccgaa gtggtcgccc aggagaacga gcggctcaag
     1621 tccgaagccc agaaaaacac caacgtcgcc aaacagcagt acgccgccga aatctccaag
     1681 cagacggcga tcacggaaca ggccgggccg ctggcgaccg ccggcgaacg gcaaaaggtc
     1741 attgtcgccg aggtcaagat cgaggaggaa cgctcgcggg cccagatcgc ggtggaagag
     1801 cagaacatcc tgcgcgaaca gaaggcgcaa gaggccgagg tcgtggtgcc ggccaaggcc
     1861 aaggcggact cgcaggtcaa ggaggccgag ggttaccgcc aggcgcagat ctcgcgggcc
     1921 gacggcgaca agtacgcgac gatcgcgcgg gccgacgccg
//
LOCUS       DAOOKS010000399         1957 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_73624_length_1957_cov_3.242902, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000399 DAOOKS010000000
VERSION     DAOOKS010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73624_length_1957_cov_3.242902"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="PK112_06785"
     CDS             complement(<1..197)
                     /locus_tag="PK112_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HPC11791.1"
                     /translation="MKLAVIGAGLMGRAAVYDFAQGADVEQIGVYDINPELAQQVASK
                     FGGGKAKAGRLDAGDEEAAAKV"
     gene            488..>1957
                     /locus_tag="PK112_06790"
     CDS             488..>1957
                     /locus_tag="PK112_06790"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HPC11792.1"
                     /translation="MRCIRPLILGAAVALYAGASATADRPLGSAKQLSPGAVIGTSYW
                     DRQCNFATTRRVHLSTAAGRELVHHVWTFQPAPPVWERTAHYRLYDIAGGAYSPGVDI
                     PEGTRIRAGFGDMWITDDNRAVIAYQHYSDPDQRYHLWTAFDSAAGAGEFSNLVEIPD
                     SIDFYSLVNYQSPLWPHVAYVEGVHDTVLHIVAQESLQYPDSVAIFYFRRVGSDSDPT
                     AVWDYPPRVIDSNYNWGSHQLAATSDGRVAIAWTANVPCAGDPDTASGVGCSDFVFWE
                     NDLYFQVSNDQGLTWQNRVNVTRYSETPEHPYRAHWEISALFDHLGVLHMIWSAAAWP
                     RTAPGPGEVAQTASAIFHWSETNPTPTMVRETNWNQTVCGGGEGRPLNIGDISLAECD
                     GRLYTLFVQFNDYDAGIMSDCCDSNLTFYPDGAANGDLYICVSSDGGLTWDAARNLTS
                     TRTPGCRFDTPGNVCACESWPSVTPYGSNVSIDPSISGAD"
BASE COUNT          345 a          667 c          569 g          376 t
ORIGIN      
        1 actttggccg ccgcctcttc gtccccggcg tccagccgcc ccgccttggc tttcccgccc
       61 ccgaatttcg atgctacctg ctgggccagt tccgggttga tgtcgtagac gccgatctgt
      121 tcgacgtcgg ctccctgcgc gaagtcatag acggcggccc ggcccatgag cccggcgccg
      181 atcactgcca gcttcatact gtcctctccg gtcgaatcaa aaccatcctc gcttgcaata
      241 gctcctaata taacgccccc gccccctccc gccatcacaa atcggctgaa aatatacaca
      301 gtgtgggaaa cgtgaatgtg attgacaagt tgctccccgc ttgtcaggat gcagccgaca
      361 ccgtctcgca cacggatgtc atgatctggg agaaacagag gtccgcttac cggcggactc
      421 aaggagggat tttcgtggcc gtcgcggtcc gctgcggcgg cgaacaccaa cacttcagga
      481 gggagatatg cgctgcatcc gcccgctcat attgggcgcc gccgtggcgc tgtacgccgg
      541 cgcgtcggcc acagccgacc gcccgctcgg ttcagcgaaa cagttgtcgc cgggcgccgt
      601 tatcggcacg tcttattggg atcggcaatg caatttcgcc accacgcgcc gcgtccacct
      661 gagcaccgcg gccgggcgcg agctcgtcca tcatgtgtgg acctttcaac cggcgccgcc
      721 cgtgtgggaa cggaccgctc actaccgtct gtacgacatc gcgggcggcg cctacagccc
      781 gggggtggac atacccgagg gcactcgcat cagggccggc ttcggggata tgtggatcac
      841 cgacgacaac cgggcggtga tcgcgtacca acactattcc gacccggacc aacgatatca
      901 tctgtggaca gcctttgact cggcggcggg cgccggcgag ttctccaatc tggtcgagat
      961 ccccgactcc atcgacttct acagcttggt caactaccaa tcgcccctct ggccccacgt
     1021 ggcctacgtg gagggtgtcc acgacacggt ccttcacatc gtggcgcagg aatcgctcca
     1081 gtatccggac tcggtcgcga tcttctactt ccgccgcgtc ggatccgatt ccgacccgac
     1141 ggcggtgtgg gattacccgc ctcgcgtgat cgattccaac tacaactggg gttcccacca
     1201 gcttgcggca acctccgatg gccgggtggc tatcgcgtgg acggccaacg tgccgtgcgc
     1261 gggggatccc gacaccgcct cgggagtcgg ctgctctgat ttcgtctttt gggagaacga
     1321 tctgtacttc caggtgtcga acgaccaggg tctcacgtgg cagaatcggg ttaacgtgac
     1381 ccgttactcc gaaacgccgg agcaccccta tcgggcgcac tgggagatat cggcgctctt
     1441 cgatcacctt ggcgtcctgc acatgatatg gtcggctgca gcctggccgc gaacagcccc
     1501 cgggcccggt gaggtcgccc agaccgccag cgccattttc cactggtcgg agaccaaccc
     1561 gacgccgacc atggtgcgtg agacaaactg gaatcagacc gtctgcggcg gcggcgaggg
     1621 ccgcccgttg aacatcggcg acatatcgct cgccgagtgc gacggacgtc tctacaccct
     1681 gttcgtccag ttcaacgatt acgacgccgg cattatgagt gactgctgtg actcgaactt
     1741 gactttttac cccgatggcg ccgccaacgg cgacctgtat atctgtgtct cctcggacgg
     1801 cggactgacc tgggacgctg cccgcaatct gacgtccacg cgaactcccg gctgcaggtt
     1861 cgacacgccc ggcaacgtct gcgcgtgtga aagctggccc tcggtcaccc cctacgggag
     1921 caatgtctcc atcgacccgt caatttccgg ggccgac
//
LOCUS       DAOOKS010000400         1955 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_73747_length_1955_cov_2.280526, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000400 DAOOKS010000000
VERSION     DAOOKS010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73747_length_1955_cov_2.280526"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..1878)
                     /locus_tag="PK112_06795"
     CDS             complement(103..1878)
                     /locus_tag="PK112_06795"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HPC11793.1"
                     /translation="MICCGPGCLANGATKLVQAFRSELEKKRIRDFRVEALKETGCHG
                     FCEKGPLVVIEPQGTFYTRVTPADVPEIIEQSIRGNQTVERLLYRDQATGEPKTTYHE
                     IDFYRKQTRIALRNLGKIDQYSIDEYIRVGGYQALAKVLTTMTPDQVITEVKASGLRG
                     RGGAGFHTGRKWESCATADDWPHYVVCNGDEGDPGAFMDRSIMEGDPHSVLEGMIICA
                     FAVKSKDGYIYVREEYPLAVQNLQRAITQARAYGLLGEGILGTEFSFDIKISRGAGAF
                     VCGESSALMKSVAGEVGEPRAKYVRSVLKGLYDKPTVLNNVESYANIPVIIAKGGAWL
                     AAIGTPKSTGTKAFSLVGKVRNTGLIEVPMGTTLREIIYGIGGGILHDRPFKAVQTGG
                     PSGGCLPADKLDLPVDFDTLTDAYSMMGSGGMIVMDERTCVVDVAKYFLHFLVEESCG
                     KCTPCREGLFQLHELANRISDGRGTEADLALMEKLSENIIIGSLCGLGKSGPNPFVST
                     IRYFREEYLAHIRDHKCPAGVCTALIRYDIVAEKCDGCMACIHACAVNAITGRKGKVH
                     VINQDICTKCGACMAVCRRDAVEVA"
BASE COUNT          310 a          683 c          585 g          377 t
ORIGIN      
        1 gaaatgccag ccgcgcatat aggtatggcg caccagcagc atctcgccct tctccgcccg
       61 cactgcccgt ccgttgactt gtatcgtaac cgttgccatg agctatgcca cctccacggc
      121 atcccgccgg cacaccgcca tacaggcgcc gcacttggta cagatatcct ggttgatcac
      181 gtgcaccttc cccttccgcc cggtgatcgc gttcaccgcg caggcatgga tgcacgccat
      241 gcagccgtcg cacttctccg ccacaatgtc atagcggatg agggccgtgc agacgcccgc
      301 cgggcacttg tggtcccgga tgtgcgcgag gtactcctcg cggaagtacc ggatcgtgct
      361 cacgaaggga ttgggacccg acttgccgag cccgcagagc gagccgatga tgatgttttc
      421 cgagagtttc tccatcaggg cgaggtcggc ctcggtcccg cggccgtcgc tgatccggtt
      481 ggccagctcg tgcagttgga agagcccctc acggcagggc gtgcacttgc cgcatgattc
      541 ctcgacgagg aagtggagga agtacttggc cacgtccacc acgcaggtcc gctcgtccat
      601 gacgatcatc ccccccgatc ccatcatcga gtaggcgtcg gtcaacgtgt cgaaatcgac
      661 cggcaggtcg agcttgtcgg ccgggaggca gccgcccgag gggccgcccg tctgcaccgc
      721 cttgaacggc cggtcgtgga gaatcccgcc gccgataccg tagatgatct cgcgcagcgt
      781 cgtccccatc ggcacttcga tcaggccggt gttgcgcacc ttgccgacca gcgagaacgc
      841 tttcgtcccg gtgctctttg gcgtgccgat cgcggccagc caggcgccgc ccttggcgat
      901 gatcaccggg atgttggcgt aggactcgac gttgttgagc actgtcggtt tgtcgtacag
      961 ccccttgagc accgaccgga cgtacttggc ccgcggctca ccgacctcgc cggccaccga
     1021 tttcatcagg gccgatgatt cgccgcagac gaaagccccc gccccgcggc tgattttaat
     1081 gtcgaacgag aactccgtgc cgaggatgcc ctcgccgagg agcccgtacg cgcgtgcctg
     1141 ggtgatcgcc cgctgcaggt tctgcacggc cagcgggtac tcctcccgca cgtaaatgta
     1201 gccgtctttc gatttcaccg cgaaggcgca gataatcatc ccctcgagca cgctgtgcgg
     1261 atccccctcc atgatcgagc ggtccatgaa cgccccgggg tcgccctcgt cgccgttgca
     1321 cacgacgtag tgcggccagt cgtccgccgt ggcgcacgac tcccacttgc ggccggtgtg
     1381 gaaaccggcg ccgccccggc cgcgcagccc cgaggccttc acctcggtga tcacctggtc
     1441 gggcgtcatc gtcgtcagca ccttcgccag cgcctggtac ccgcccaccc ggatgtactc
     1501 gtcgatcgag tactggtcga tcttcccgag atttcgcagc gcgatgcgcg tctgcttgcg
     1561 gtaaaaatcg atctcgtggt aggtcgtctt gggctcgccg gtggcctggt cccggtagag
     1621 cagccgctcg accgtttggt tgccgcggat cgactgctcg ataatctccg gcacgtcggc
     1681 gggcgtcacc cgcgtgtaga acgtcccttg cggctcgatc acgacgagcg gccccttctc
     1741 acagaatccg tggcagccgg tctccttgag cgcctcgacc cggaagtccc tgatccgttt
     1801 cttctccaat tccgagcgga acgcctgcac gagcttggtg gctccattag ccagacagcc
     1861 cggcccgcag cagatcatga ccttcttgcc aagtttctta tcctgcgccg ccaggtcggt
     1921 cttccaggtg ccgtagctgc ccgccgagat gcgcg
//
LOCUS       DAOOKS010000401         1953 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_73855_length_1953_cov_2.254478, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000401 DAOOKS010000000
VERSION     DAOOKS010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73855_length_1953_cov_2.254478"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1886)
                     /locus_tag="PK112_06800"
     CDS             complement(<1..1886)
                     /locus_tag="PK112_06800"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HPC11794.1"
                     /translation="MTFRMGFLTLAAVMLGAAPVVADKLVEVTLRDFAEAQQLEAAGA
                     TAVAAAGNLYLAVISDSGLARLTAAGVEAAVVAQGVAADRLAIDRSAPGAYRGPHAVV
                     FARDRLRLVQVDSRAAAEKDPELVPITNFPVPIRYSPARPAPQPAAGAMDTIDSLVAL
                     VSEDSLRSYTERLQAFYRRVSDTDSSRAARDWLAAKFAAFGYDSVVLDYFDPYPSSST
                     PPGECCNVVACRPGAAYPEQQIVIGAHYDAVASSPGADDNASGTAAVLELARVLAGCA
                     APVTLTYIPFDAEESGLLGSRHYADSMAALNREIVLMVNFDMIGHETNDSVARVYFNH
                     DSVYADLWVLLAPTTGPLVGDIAGYGGRSDHVPFGSNGYDFLSLTEDDFSTHYHSPSD
                     STTYLNFDYVERIVRVSLAAVYAAATLPPPVPIAAIRQPGDGRSLLVEWRPLRRTALD
                     HYRLCWYRQGSPATLQCLDLPPSDSIHTVEGLTEGAAYIFYVQAFDSAGQTSLTWPDG
                     IGIPQSRPAPPEGLTGLPIHQGVALQWGRNNQELDLSHYAIYRDGALAGTTSDTVFQD
                     QDPGLGEEFHEYLVAAVDDDGIVSDTIGRTPFEARAATLTPGRILAINRDNVAVPGAE
                     VALTG"
BASE COUNT          323 a          668 c          668 g          294 t
ORIGIN      
        1 ccggtgagcg cgacctcggc tccgggaacc gcgacattgt cgcggttgat cgccaatatc
       61 cgccccggcg tcaaggtcgc cgcccgggcc tcgaacggcg tccggccgat cgtgtcggag
      121 acaatgccgt cgtcatccac ggccgcgaca aggtactcat ggaactcctc ccccagcccc
      181 ggatcctggt cctgaaaaac cgtgtcggaa gttgtgcccg caagcgcgcc gtcgcggtag
      241 atcgcgtagt ggctcaaatc cagttcctgg ttgttccgac cccactgcag cgccacgccc
      301 tgatgaatcg gcagtcctgt cagcccctcc ggcggcgccg gccgcgactg cgggatgccg
      361 atcccgtcgg gccacgtcag cgaggtctgt cccgcggaat cgaacgcctg cacgtagaag
      421 atgtacgcgg ccccttcggt cagcccctcc acggtgtgta tgctgtcgga aggagggagg
      481 tcgagacact ggagggtcgc ggggcttccc tggcggtacc agcagaggcg gtagtgatcg
      541 agcgcggtcc ggcggagggg gcgccactcc acgaggagcg accggccgtc gcccggctgc
      601 cggatcgcgg ctatcggcac cggcggcggc agcgtcgccg ccgcatacac ggcggccagc
      661 gacacgcgca cgatccgctc cacgtaatcg aaattgagat aggtcgtcga atcgctcggc
      721 gagtggtagt gggtggagaa gtcgtcctcg gtcagactga ggaagtcgta gccgttgctc
      781 ccgaacggca cgtggtccga tctcccgccg tacccggcga tatccccgac gagcggtccg
      841 gtagtcgggg cgaggagcac ccacagatcg gcgtagaccg aatcgtggtt gaaatacacg
      901 cgcgcgaccg aatcgttcgt ctcgtgcccg atcatgtcga aattaaccat gaggacgatc
      961 tcgcggttga gcgccgccat cgaatcggcg tagtggcgcg agcccagcag gccgctctcc
     1021 tcggcgtcga acgggatata cgtcagagtc acgggcgccg cgcacccggc gagaacgcgg
     1081 gcgagttcga gcaccgccgc cgtccccgag gcgttgtcgt cggcgccggg gctgcttgcc
     1141 accgcgtcgt agtgcgcgcc gatcacgatc tgctgttcgg gatatgccgc gcccggccgg
     1201 caggccacca cgttgcagca ctcgccgggg ggcgtgctcg atgaggggta gggatcgaaa
     1261 tagtcgagca ccaccgaatc gtagccgaac gcggcaaact tagccgccag ccagtcccgc
     1321 gccgcccggc tcgagtcggt gtcggacacg cggcggtaga atgcctgcag ccgctcggtg
     1381 taactgcgca gcgagtcctc gctcaccagg gcgaccaggc tgtcgattgt atccatggcg
     1441 ccggcggccg gctgcggcgc cgggcgcgcg ggggagtagc ggatcggcac ggggaagttg
     1501 gtgatcggaa ccagctcggg gtccttctcg gcggcggcgc gagaatcaac ctgcaccagc
     1561 cggagtcgat cgcgcgcaaa caccaccgcg tgcggtcccc ggtacgcgcc gggggcgctg
     1621 cggtcgatcg ccagacggtc ggctgcgacg ccctgggcta cgacagcggc ctccacgccg
     1681 gccgcggtca ggcgggccag gcccgagtcg gagatcaccg ccaggtacaa gttccccgcc
     1741 gccgccaccg ccgtcgcgcc ggcggcctcg agctgctggg cctcggcaaa atcccgaagc
     1801 gtcacttcca ccaatttgtc ggcgactacc ggggcggcgc cgagcatcac cgccgctagc
     1861 gtcaggaatc ccatgcggaa cgtcatatcc actttcctcc aacgccaccg cccgccggcg
     1921 ggctacttga ggaggagcat tttcctggca gcg
//
LOCUS       DAOOKS010000402         1952 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_73884_length_1952_cov_3.515551, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000402 DAOOKS010000000
VERSION     DAOOKS010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73884_length_1952_cov_3.515551"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..959
                     /locus_tag="PK112_06805"
     CDS             <1..959
                     /locus_tag="PK112_06805"
                     /inference="COORDINATES: protein motif:HMM:NF014715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - glucose binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HPC11795.1"
                     /translation="VSRRTVRLAAVTEAQGRVTLKDQARFDAADFDSFETILQRYFSR
                     RRTEGALFFVAVAGLPVGSTVSSALLPWAIDGDSIRAHFSFDVVRLVNEHLATARGLF
                     DLPPDALFTINDQPAVPGNIGLMAVDELLAEVLMVRRDNGYLSFLTNGGHTGLAPATQ
                     LEAELWQYLYAQRDVVEVGDVVSSAGLVRLVDFYLESHGHEVPEWLQNAPDPPSRIVE
                     TALAGKQQVAVDAVDLFVDCFAGEAANLALRGATTGGLYLAGRVTTELLPSLDQGRFL
                     DNYLKRGAAEEKLRPIPIRVILDSAAPLRGVARMALEFEHPQ"
     gene            956..1624
                     /locus_tag="PK112_06810"
     CDS             956..1624
                     /locus_tag="PK112_06810"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HPC11796.1"
                     /translation="MTRAVAFLHGVYRRRDLPFYRALCRGRLTIAVDGGYRFFRMAGL
                     FPDILLGDFDSLKRLPANLPPRTRVLRFPSRKDKTDAHLAIECCRERGLGAIDLVMPG
                     LGEIDHALGVVFLLGLRDRPAARSGRADIRVVSPQCEIRLVADSRITYRRRGGDLVSV
                     LPLSARIRLTTEGTEYDVTEAWLQFGDTRGLRNRIVRARATVAVRGQALVVRRFSGGL
                     AARP"
     gene            1825..1895
                     /locus_tag="PK112_06815"
     tRNA            1825..1895
                     /locus_tag="PK112_06815"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1857..1859,aa:Gln,seq:ctg)
BASE COUNT          278 a          718 c          605 g          351 t
ORIGIN      
        1 ccgtcagccg gcgtactgtc cgccttgccg ccgttaccga ggcccagggg cgcgtcaccc
       61 tgaaagacca ggcccgcttt gacgcggcgg acttcgacag tttcgaaact attctccagc
      121 gctatttcag ccggcggcgc accgagggcg cgctcttctt cgtcgccgtg gccggcctcc
      181 cggtcggctc gaccgtctcc tcggccctgc tgccatgggc gatcgacggc gacagcattc
      241 gcgcgcactt ctcatttgac gtcgtccggc tcgtgaacga acacctcgcc accgcgcgcg
      301 gtctgttcga cctgccgccc gatgcgctct tcacgatcaa cgaccaaccg gcggttcccg
      361 gcaacatcgg tctcatggcg gtcgatgaac ttctcgccga ggtgctcatg gtgcgccggg
      421 acaacggcta cctcagcttc ctcaccaacg gcggccacac cggtctcgcc cccgccaccc
      481 agctcgaggc cgagttgtgg cagtatctct acgcccagcg ggatgtggtc gaggtcggcg
      541 acgtggtcag ttcggcgggg cttgtccggc tcgtggactt ctatctcgag tcgcacggcc
      601 acgaagtgcc ggagtggctg cagaacgccc cggacccgcc ctcccgcatc gtcgagacgg
      661 ccctcgcggg aaaacagcag gtggctgtcg acgccgtcga cctgttcgtc gactgcttcg
      721 cgggcgaagc ggccaacctc gccctgcgcg gcgcgactac gggcgggctc tacctcgccg
      781 gccgcgtcac caccgagctg ctcccctcgc tcgaccaggg ccgctttctg gacaactacc
      841 tcaagcgcgg ggccgccgag gagaagctgc gtcccattcc catccgggtg attctggaca
      901 gcgccgcgcc gctgcgggga gtcgcccgca tggctctgga attcgagcac ccgcaatgac
      961 gcgcgccgtt gcgttcctcc acggcgtcta ccgccgccgc gatcttccgt tctaccgcgc
     1021 gctctgccgc gggcggctga cgattgcggt cgacggcggt taccgctttt tccggatggc
     1081 tgggctgttt cccgacattc tgctgggcga ttttgactcg ctgaaacgcc tccccgccaa
     1141 cctcccgccc cgcacccgcg tcctgcgatt cccgtcccgc aaggacaaga ccgacgccca
     1201 cctggcgatt gagtgctgtc gggagcgcgg cctcggggcg atcgacctgg tgatgcccgg
     1261 cctcggcgag attgaccatg ccctcggcgt cgtctttctc ctcggcctgc gcgatcgccc
     1321 cgccgcccga agcggccgcg ccgatatccg tgtggtcagc ccgcaatgcg agatccgcct
     1381 cgtggccgac agccggatca cctatcgccg gcgcggggga gacctcgtct cggttctgcc
     1441 gctctcggcg cgaatccggc tcaccaccga ggggaccgag tacgacgtga ctgaggcttg
     1501 gctgcaattc ggcgataccc gggggctgcg caatcgcatc gtccgcgccc gcgcgacggt
     1561 cgctgtccgg gggcaagcgc tggtggtgcg ccgtttttcc ggcggcttgg cggcccgccc
     1621 ataaggcggt gtctcccatc gggaaaactg ctgtcgcggc cgcccagccg cccggcgcgc
     1681 gccggcccgg cgctcccgac tctcccccgc aaaattgccc cccgtttatc ttgacaacag
     1741 ggtgcagatg cctatattca gggcctgtaa aaatcggccg ggcatccaga gatcgttgtg
     1801 tcctgccgac aagaccgtag acactgggcg atcgtccaat ggtaggacat gcggctctgg
     1861 accgtagaat cggggttcga atccctgtcg cccagctttt ttatgcccgc cgcgcccggc
     1921 gctctcagcc gaacgtcgcc aacagcaccc at
//
LOCUS       DAOOKS010000403         1950 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_74021_length_1950_cov_2.620053, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000403 DAOOKS010000000
VERSION     DAOOKS010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74021_length_1950_cov_2.620053"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1663)
                     /locus_tag="PK112_06820"
     CDS             complement(<1..1663)
                     /locus_tag="PK112_06820"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HPC11797.1"
                     /translation="MNDNVKRLRLGAIAVLLAVAGYYAYDRLTPPTRIDKLAQIIHLE
                     DRRELSGRLKSYLGDSDPAVRARAALAVGRIGGPGSAEPLLDLLADSSWEVAGAAATA
                     IGFCGQPEFALRLLETADNLPPRITARAVESAGRLADTGKAEVIDALLAYLSHPAPEV
                     RERAVRGLFRAGARSAAAEVIGLYGEDFDEPVRQACLYFLARFDIAAGKEIFASYLAD
                     PDPNLRGLAVRGMGAVAGEEAERYVSIALNDNTDRVVAQAIAELAGRDPDRVSRLLQK
                     KLAVEEDPKLIVELIEALRRLGSSEGIATAEEALAAYATPSIAAATVKYAAAARGGRA
                     MDLIDSLLRGDSPFERAAAAEALGLIRERTVLPRLTTLLADRSPQVRAAAFGMLMETD
                     SANGDFYIDQALHDSDFVLQALGVEAAGKRGDPQYLPLFRTLMEPAAGTAADVRRSVV
                     GALPPFLSSRADSAARELLRLALRDAEAAVRRDASQVWDSLVTAPKPAVSLLADTRLS
                     ERAIARALAGYAANPRALISTSRGNLAIELRFDVAPLTVMNFIELA"
     gene            1815..>1950
                     /locus_tag="PK112_06825"
     CDS             1815..>1950
                     /locus_tag="PK112_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HPC11798.1"
                     /translation="MYKPTVMDHFQNPRNTGDLDRPDGIGQVGNPACGDVMRIFIRVE
                     A"
BASE COUNT          287 a          760 c          629 g          274 t
ORIGIN      
        1 ccgccagttc aatgaaattc atcaccgtca acggcgcgac gtcaaagcgc agctcgatcg
       61 ccaggttccc ccggctggtg ctgatcaggg cccgcggatt ggccgcgtat ccggcgagcg
      121 cccgcgcgat cgcccgttcg ctcagccttg tgtcggccag cagcgacacc gccggcttcg
      181 gcgccgtcac gagcgagtcc cacacctgcg aagcatcccg ccggacagcc gcctccgcat
      241 cgcgaagggc gagccgcagg agttcccggg ccgccgaatc cgcccgggag gacaaaaacg
      301 gcgggagcgc gccgaccacc gaccgccgca cgtccgccgc ggtccccgcc gccggctcca
      361 tcagcgtgcg gaacagaggc aggtactgcg gatctccccg cttccccgcg gcctcgacgc
      421 cgagcgcctg caggacgaaa tcgctgtcgt ggagcgcctg gtcgatatag aaatcgccgt
      481 tggcgctgtc ggtctccatg agcattccga acgcggccgc ccggacctgc ggcgaccgat
      541 ccgccagcag cgtggtcagg cgcggcagca ctgtccgctc gcggatcagc cccagcgctt
      601 cggccgccgc ggcccgctcg aacggactgt cgccgcgcag cagcgaatcg atcaggtcca
      661 tcgcccgccc cccgcgcgcg gcggcggcgt acttcaccgt cgcggccgcg atcgacggcg
      721 tcgcgtaggc cgccagcgcc tcctccgccg tcgcgattcc ctcgctgctg cccagccggc
      781 ggagagcctc gatgagttcg acgatgagct tgggatcctc ctccaccgcg agcttcttct
      841 ggagcaggcg actgacccgg tcggggtcgc gcccggccag ttcggcgatc gcctgcgcca
      901 ccacccggtc cgtattgtcg ttcagggcga tgctcacgta gcgctcggcc tcttcgccgg
      961 cgaccgcgcc catcccgcgc accgccagcc cccgcaggtt cgggtcgggg tcggcgaggt
     1021 aggacgcgaa gatctccttg ccggcggcaa tgtcgaagcg ggccaggaag tacaggcagg
     1081 cttgccgcac cggctcgtcg aaatcttccc catacaggcc gatcacctcg gccgcagccg
     1141 accgggcccc cgcccgaaag agcccccgca cggcccgctc ccggacctcg ggcgcgggat
     1201 gcgacaggta ggccagcagc gcatcgatca cctccgcctt gccggtgtcg gccagccgcc
     1261 cggccgactc gaccgcccgg gctgtgatcc gcgggggcaa gttgtcggcc gtttccaaca
     1321 ggcgaagagc gaactccggc tgtccgcaga atccgatcgc ggtggccgcc gcccccgcca
     1381 cctcccaact gctgtcggcc agcaggtcca ggagcggctc cgccgatccc ggcccgccga
     1441 tgcggccgac cgccagggcc gcccgcgcgc gcaccgccgg gtcgctgtcg cccaagtagc
     1501 tcttcagccg ccccgacagc tcgcggcggt cttccaggtg gatgatctgc gccaatttgt
     1561 cgatgcgggt gggcggggtg agccggtcgt aggcgtagta cccggccacc gccagcagca
     1621 cggcgatcgc ccccagccgg agcctcttca cattgtcgtt catagaggga gaaggtacgg
     1681 ccgggcttcc cccccgtcaa tcgtttagac accagccacg tgtgccgccc ggcagcgcgg
     1741 ccgcggttcg ccggcgttcc gccctcccgc gcttgtcccc ttgcccgcag cgccccccgg
     1801 ccgtattatg cagcatgtac aagccgacgg tcatggacca cttccagaat ccccgcaaca
     1861 ccggcgacct cgaccgcccc gacggcatcg ggcaggtcgg caacccggcg tgcggcgacg
     1921 tcatgcgcat cttcatcaga gtcgaggccg
//
LOCUS       DAOOKS010000404         1949 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_74076_length_1949_cov_2.653115, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000404 DAOOKS010000000
VERSION     DAOOKS010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74076_length_1949_cov_2.653115"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="PK112_06830"
     CDS             <1..452
                     /locus_tag="PK112_06830"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPC11799.1"
                     /translation="FNRFRQTLGITTIETGVAARGVLRALRENKVVGIAGDQHSHTGL
                     RISFFGRPATASRGPALFALKAGAPLLPYMMRRESYDRHVITAGEPIYPPGGGDEEKD
                     ADEMTLRLHRFYEAEIRKYPDQWMWTHRRWKAAEGEEKAATFREHMQ"
     gene            531..1811
                     /locus_tag="PK112_06835"
     CDS             531..1811
                     /locus_tag="PK112_06835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPC11800.1"
                     /translation="MKKYIYIGIILALVLGGYLAVNAIFTPAVDIPTATARRGDMVIS
                     LNENGTVNAKRAMTLSSPRIRSLTITWLAPEGSTVKEGDPVIRFDATQQLADLQENQS
                     SLKIAQTALERANKEYTIQEKQLTLDLEKARRNHLEKKHEAPLIAEEAKMELDLAELN
                     FQAKLEQLRSDVQKAELEVQRAQEKVNQAQRELDQMTVAAPIPGLVVYLEIWKGSSMG
                     KVQEGDAPWPGQGLVNLPDLSDMMVETTVSEVDANKVDTGQEVVVTLDAFPEVQFQGR
                     VAQKSTLARKKEQNSKINVFDIEVDITQEDPRLKPGMSAACKIVVDRLQDVVSVPLEA
                     VFERDDTTVVFLKDKKKRPVAVGRRNDMFIEVRDGLRGGEEICLVDPTVQEQGLPGDR
                     ATEPELNRDRTAAPSAPPAGGEGRRTGGGRRGGS"
     gene            1880..>1949
                     /locus_tag="PK112_06840"
     CDS             1880..>1949
                     /locus_tag="PK112_06840"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11801.1"
                     /translation="MLGVIFGVGAVISMLSIGEGAKQ"
BASE COUNT          398 a          600 c          677 g          274 t
ORIGIN      
        1 tgttcaaccg gttccggcag acgctgggga tcacgacgat cgagacgggg gtggcggcgc
       61 gcggtgtgct gcgggcgctc cgcgaaaaca aggtagtggg gatcgccggc gaccagcaca
      121 gccacaccgg gctgcgcatc tcttttttcg gccgcccggc gaccgcctcg cgcgggccgg
      181 cgctgttcgc tctcaaagcc ggggccccgc tgttgcccta catgatgcgg cgggagagct
      241 atgaccgcca cgtcatcacc gccggcgaac cgatctaccc gcccggcggc ggcgacgagg
      301 agaaagatgc ggacgagatg acgctccggc tccaccggtt ctacgaggcg gagattcgca
      361 agtacccgga ccagtggatg tggacgcacc ggcggtggaa agcggccgag ggggaggaaa
      421 aggcggcaac ctttcgggag catatgcagt agaaaccgga taagcggcat gcccggcgcc
      481 gcagggcgcc gggggatgat gacaagcaag cgactaacat agaggtcggc gtgaagaagt
      541 acatttacat cggtatcatc ctcgccctcg tgctcggggg ctatctggcg gtcaacgcga
      601 tcttcacccc tgcggtggac atcccgacgg cgaccgcccg gcgcggcgac atggtcatct
      661 cgctcaacga gaacgggacg gtcaatgcga aacgggcgat gaccctctcc tcgccgcgca
      721 ttcgcagcct caccatcacc tggctcgcgc ccgagggatc gaccgtgaaa gagggggatc
      781 cggtgatccg gttcgacgcc acgcagcagc tggcggacct ccaggagaac cagtcgagcc
      841 tgaagatcgc ccagacggcg ctcgagcggg caaacaagga gtacacgatc caggaaaagc
      901 agctcaccct cgatctggag aaggcccgcc gcaaccacct ggagaagaag cacgaggccc
      961 cgctcatcgc cgaagaggcc aagatggagc tcgatctggc cgagctcaat ttccaggcca
     1021 agctcgaaca actccggtcg gacgtgcaga aggcggaact cgaggtgcag cgcgcccagg
     1081 agaaggtcaa ccaggcgcag cgcgaactgg accagatgac ggtggccgcg cccatccccg
     1141 gactggtcgt ctacctggag atctggaaag gcagctcgat gggcaaggtg caggagggag
     1201 acgctccctg gccgggccag gggctggtca accttcccga cctcagcgac atgatggtcg
     1261 aaaccacggt ctcggaggta gatgcgaaca aggtcgacac cgggcaggaa gtggtcgtga
     1321 cgctcgatgc gttcccggag gtgcagttcc aggggcgggt ggcgcaaaaa tcgaccctgg
     1381 cgcggaagaa ggagcagaac tccaaaatca acgtgttcga tatcgaggtg gacatcaccc
     1441 aggaagaccc gcggctgaaa ccggggatgt cggccgcctg caaaatcgtg gtcgataggc
     1501 ttcaggacgt cgtctcggtg ccgctggagg cggtgtttga gcgggatgac acgacggtgg
     1561 tgttcctcaa ggacaagaag aagcggccgg tggcggtggg ccgccgcaac gacatgttca
     1621 tcgaagtgcg cgacggcctg cgggggggcg aggagatctg cctggtcgac ccgaccgttc
     1681 aggagcaggg actgcccgga gaccgggcga ccgagccgga gctcaaccgc gaccgcactg
     1741 ccgcgccatc ggccccgccg gccgggggcg aagggcgccg gacgggcggc gggaggcgcg
     1801 gcggatcatg aacttccacc gcatcttcca gatctccttc gactcgctgg ccgcccacaa
     1861 gctgcggact ttcctcacca tgctcggcgt cattttcggc gtcggcgcgg tcatctccat
     1921 gctctcgatc ggcgaagggg ccaagcagg
//
LOCUS       DAOOKS010000405         1947 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_74184_length_1947_cov_3.329281, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000405 DAOOKS010000000
VERSION     DAOOKS010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74184_length_1947_cov_3.329281"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="PK112_06845"
     CDS             <1..432
                     /locus_tag="PK112_06845"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPC11802.1"
                     /translation="LVSTGVVLPDLARRLPAAGAAAPPQHLIAWETDLPRGLARAQGE
                     GKPVVIDTWATWCANCRVLEKKTFGNPDVAAKVAERFVPIKVQLESADSEATRAFMAR
                     FGMSHYSLPTTLLLDSQGTVRRVLRGVVGPDEMIAALAGVR"
     gene            895..1338
                     /locus_tag="PK112_06850"
     CDS             895..1338
                     /locus_tag="PK112_06850"
                     /inference="COORDINATES: protein motif:HMM:NF021365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2064 domain-containing protein"
                     /protein_id="HPC11803.1"
                     /translation="MERWGLRIEKVFQDAFAAGYRHVLVIGSRTPTVKTDQMCRALRM
                     LRVSDAVFGPTPEGRYYVIGMSSEYRVKLSEFDWKSPSIYHDVSNAFTEQELRWAELE
                     IWYCVEDPDELELMVRDINQFRFEGDELSARETELVIERLLSRME"
     gene            1339..>1947
                     /locus_tag="PK112_06855"
     CDS             1339..>1947
                     /locus_tag="PK112_06855"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HPC11804.1"
                     /translation="MVERRLQRLSWLRIRELVPAEIDTAVLPVGTVEAHGAACLGTDI
                     YIPEDIAGHIAERLNALVAPTVTYGITKSLLRYNGGATVRPETFGAYVRDVLDSLAAA
                     GFRHVIVINGHGGNNAVLKDVAYAFHAERRANIAVIHWWQLCADMTENFFGHIGGHAG
                     TDETAYIWAMDPALADRERYDPDLAYTFNPGADVYPVPGTILL"
BASE COUNT          332 a          632 c          664 g          319 t
ORIGIN      
        1 ctggtgtcga cgggggtggt gctgcccgac ctcgcccggc ggctcccggc cgcgggcgcc
       61 gcggcgccgc cgcagcacct gatcgcctgg gaaaccgatc tcccgcgggg cctggcgcgc
      121 gcccagggcg agggaaagcc ggtggtcatc gacacctggg cgacgtggtg cgcgaattgc
      181 cgcgtgctcg agaagaagac tttcgggaat ccggacgtgg ccgcgaaggt ggcggagcgg
      241 ttcgtgccga tcaaggtcca gctcgagagc gccgacagcg aggccacgag agcgttcatg
      301 gcgcgcttcg gcatgagcca ctactcgctg ccgacgacgc tccttctgga ttcgcaggga
      361 acggtgcggc gggtgctccg cggcgtggtc gggccggacg agatgatcgc ggcgctggcc
      421 ggcgtgcgct gaccccgccc cgcttcaggc gccgcggccc gctccctctc cgcgcgcttg
      481 cgcgcccgct ctccccgacc gcttttccgg aaattctctt tcggcgaacc ttgacgccgc
      541 cgaatcccgg catgtatatt ccggcgtgac agaggaccct aactcgtaag ggacgtatgg
      601 cccggacaaa gaagaacgat tcagtcatcg ccgtgtgcgt ccaggagccg actgaagacg
      661 gatcgcagat ggagctcggc gccatccagg gcgattccct gcgctttctc caccaggctt
      721 tcattaccga cacgatcaac cacgctctcg atgccgatgt cgacgtgcgc ctgtactatg
      781 ccgaactgcc ggagcgcgag cgattggtcg ggatcataac ggagtacctc aggaagaaat
      841 cgagcggcaa gcgggcgacc gcgctgaaaa atcggttcca gggtgaagct gccgatggag
      901 cggtggggcc tgcgaatcga gaaggtgttt caggacgcct tcgcggccgg ctacaggcac
      961 gtgctcgtga tcggcagccg gacccccacg gtcaagaccg accagatgtg ccgcgccctg
     1021 cgcatgctgc gggtgtcgga cgccgtgttc gggcccaccc ccgaggggcg atactacgtg
     1081 atcggaatgt cgtcggagta ccgggtgaag ctgtccgagt tcgactggaa atcgccgtcg
     1141 atctaccacg acgtgtcgaa cgcgttcacc gagcaggagc tgcgctgggc ggagctggaa
     1201 atctggtact gcgtcgagga tcccgacgaa ctggaactca tggtgcggga catcaaccag
     1261 tttcgcttcg agggagacga gctgtcggcc cgcgagacgg agctggtgat cgagcgcctg
     1321 ctttcgcgga tggagtgagt ggtggaacga cgcctgcagc gcctgagctg gctgcgcatc
     1381 agggaactgg tgccggcgga gattgacacg gccgtgctgc cggtggggac ggtggaggcg
     1441 cacggcgcgg cctgcctcgg caccgatatc tacatccccg aggacatcgc cggccatatc
     1501 gccgagcgcc tcaacgcact ggtggcgccg acggtcactt acggcattac gaagtccctc
     1561 ctgcgctaca acggcggcgc aaccgtccgc ccggagacct tcggcgccta cgttcgcgac
     1621 gtgctcgact cgctggccgc cgccggcttc cgccacgtca tcgtcatcaa cggccacggc
     1681 ggcaataacg ccgtcctgaa agacgtcgcg tacgccttcc acgccgagcg ccgggcgaac
     1741 atcgcggtca tccactggtg gcagctgtgc gcggatatga ccgagaattt ctttggccac
     1801 atcggcgggc acgccgggac cgacgagacg gcgtacatct gggcgatgga tcccgccctg
     1861 gccgaccggg agcggtacga tccggatctc gcctacacgt ttaatccggg ggccgatgtc
     1921 tacccggtgc cggggaccat cctcctg
//
LOCUS       DAOOKS010000406         1941 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_74524_length_1941_cov_2.937964, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000406 DAOOKS010000000
VERSION     DAOOKS010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74524_length_1941_cov_2.937964"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="PK112_06860"
     CDS             complement(<1..355)
                     /locus_tag="PK112_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017590959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HPC11805.1"
                     /translation="MFRIESKIDTRTETFAKNDELNRAAHRLFKERLEQVKRGGPEKA
                     RARHTERGKLLPRDRVARLLDRNTPFLELSPLAAYDMYDDDAPAAGIITGIGTVHGRE
                     VMVIANDATVKGGTYY"
     gene            complement(378..>1941)
                     /locus_tag="PK112_06865"
     CDS             complement(378..>1941)
                     /locus_tag="PK112_06865"
                     /inference="COORDINATES: protein motif:HMM:NF018938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1446 domain-containing protein"
                     /protein_id="HPC11806.1"
                     /translation="VKVITTAGGINPIGLGRRLQAMVRERGLSLKVGVVQGDDIAGRL
                     VELGAAGESFVNMETGAPFAPVRDRVIAANVYLGAEPVVAALAAGCDIVVTGRVTDTG
                     ITLAPMIHEFGWAMDDWDKMAAGIVAGHIIECGAQASGGNITDWREIKSFHNIGYPII
                     EMEAGGEFAVTKHPKTGGLISEKTIKEQLVYEMGDPANYISPDGVARFDTIALRQVGP
                     DRVRVSEVKGKPAPDHLKVSMSYDDGWKASGSVLVSGPQAREKAGVIADTLWAKLDHA
                     YEATRTEVIGTGSIWPRAFATDETNEVLLRFSVRDRDYEKVRDFGKALPTLILSGPPG
                     MAVTTGGRPKPSSVVAYWPALIHRSRVKAKVLVVGTNRQESFHEITFPVRVPSPAADT
                     PAGPPRRAPGRKPAGRAVEVRLEEICYARSGDKGDTCNVGVLARSPEIYDWIAEHLTA
                     ARVQEFFAGITRGRVKRYELDNLGGLNFLLEETLGGGGTRSLMIDPQGKTLAQALLQM
                     RLRVPQSLLKTL"
BASE COUNT          279 a          708 c          602 g          352 t
ORIGIN      
        1 ggtagtacgt tccccccttg accgtcgcgt cgttcgcgat caccatcacc tcgcgtccgt
       61 gcacggtccc gatccccgtg atgatgccgg ccgcaggcgc gtcgtcgtcg tacatgtcgt
      121 aggccgccag cgggctcagt tcgagaaacg gggtgttgcg gtcgagcagc cgggccaccc
      181 ggtcgcgggg aagcagcttc ccgcgctcgg tgtgccgcgc ccgcgccttc tccggcccgc
      241 cccgcttcac ctgctccagt cgttccttga agaggcggtg cgccgcccgg ttgagctcgt
      301 cgttcttggc gaacgtctcc gtgcgcgtgt cgatcttgga ctcgattcga aacattggcg
      361 tctgtctttc cccaaggcta gagtgttttc aacagcgact gcggcacgcg cagcctcatc
      421 tgaagcagcg cctgcgcgag cgtcttcccc tgaggatcga tcatgagcga ccgcgttccg
      481 ccgccgccga gcgtctcctc gagcaggaaa ttgagcccgc ccaggttgtc cagctcgtac
      541 cgcttcaccc ggccgcgggt gattcctgcg aagaattcct ggacccgggc ggccgtcagg
      601 tgctcggcga tccagtcgta gatctcgggc gaccgcgcga gcaccccgac attgcaggta
      661 tcccccttgt cccccgaccg ggcgtagcag atttcttcga gccggacttc gaccgcccgg
      721 ccggccggtt tgcggccggg cgcgcgtcgg ggcggcccgg cgggcgtgtc cgcggccggc
      781 gacggcactc gcaccggaaa agtaatctcg tggaacgact cctgccgatt cgtcccgacc
      841 accagcactt tcgccttcac tcggctccgg tgaatcagcg ccggccagta ggcgaccacg
      901 ctcgagggtt tcgggcgacc gccggtggtc acggccatcc ccggcggccc ggagaggatc
      961 agcgtcggca gcgcctttcc aaaatcccgc actttctcgt agtcccggtc gcggaccgaa
     1021 aaccgcagga gcacctcgtt ggtctcgtcg gtcgcgaacg cccgcggcca gatcgagccc
     1081 gtcccgatca cctccgtccg cgtcgcctca taggcgtggt ccagtttcgc ccacagggtg
     1141 tcggcgatca ccccggcctt ctcgcgcgcc tgcgggcccg acacgaggac cgacccgctc
     1201 gccttccacc cgtcgtcata cgacatcgaa actttcagat ggtccggcgc cggtttcccc
     1261 ttgacctccg acacccgcac ccggtcgggg ccgacctgcc ggagcgcaat agtgtcgaag
     1321 cgcgccacgc cgtccggcga aatgtagttg gcgggatcgc ccatctcgta gaccaactgc
     1381 tccttgatcg tcttctccga gatcaggccg ccggttttgg ggtgtttggt gacggcaaat
     1441 tcgccgcccg cttccatttc gataatcggg tagccgatat tgtggaagct cttgatttcg
     1501 cgccagtcgg tgatatttcc ccccgaggcc tgcgcgccgc attcgataat gtgcccggcg
     1561 acaatccccg ccgccatctt gtcccagtcg tccatggccc agccgaactc gtgaatcatc
     1621 ggcgccagcg tgatgccggt gtcggtgacg cgcccggtca cgacgatgtc gcagccggcg
     1681 gccagcgcgg ccacgaccgg ctcggcgccc aggtagacgt tggccgcgat gacgcggtcc
     1741 cgcaccggcg cgaacggcgc gcccgtctcc atgttcacga acgattctcc ggcggcgccc
     1801 agctccacca ggcgaccggc gatgtcgtcg ccctggacca cgccgacctt cagcgacagc
     1861 ccgcgttcgc ggaccatcgc ctggaggcgc cggcccagcc cgatggggtt gatcccgccc
     1921 gcggtggtga tcaccttgac g
//
LOCUS       DAOOKS010000407         1940 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_74576_length_1940_cov_2.866313, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000407 DAOOKS010000000
VERSION     DAOOKS010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74576_length_1940_cov_2.866313"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="PK112_06870"
     CDS             complement(<1..333)
                     /locus_tag="PK112_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11807.1"
                     /translation="MYDCQHLWKMKDIQFGFVVFEKCFHCHGLRTYFTTEDNPIPGEK
                     YREGVCFWTRMEVAQSFQFNLQCALCGRKEDFSDLMGLLHCTGCLEDCPLEVLQRELH
                     AQRIWVLVA"
     gene            complement(752..>1940)
                     /locus_tag="PK112_06875"
     CDS             complement(752..>1940)
                     /locus_tag="PK112_06875"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPC11808.1"
                     /translation="SLTAPQYSELLQVRCLMLYSSNAPAPFTASVRHGAPWLTLYGAS
                     GMTNDSVCFGASAASLGQGIYVDTIDCSVEGVAAATAVPVVFYVVDDSAGPGPSAWAA
                     PPTVSITAPFGLQDLIDCHFVLSSTNAPAPYVAYAANGSAWLSVADSAGVTSDTVRLV
                     INPAAPFSAGTYVDSIACLVEGVALPVTVPVMLTLTVDSGGAPTAAVTPATVHYFGPY
                     GMLPPTTKCALVTSSNAPAPFTAWVADSVGRFTSLPDPTGVTADSLCLYLDPAGLPPG
                     IHVDTVRISVAGVSPALTLIVQLSLDMPKVSEVTNYPNPFNPETNISFSLTGATRVQV
                     TVYNTLGQEVAVLLDKRLEAGRHTVSWGGCDGAGRPAASGVYLYRITTDEGVVSRKML
                     LLK"
BASE COUNT          402 a          668 c          555 g          315 t
ORIGIN      
        1 ggccaccaga acccagatgc gctgcgcgtg cagctcccgc tgcaaaacct ccagcgggca
       61 atcttccaga caccccgtgc agtgcagcaa ccccatcagg tccgaaaaat cctccttccg
      121 cccgcacagc gcgcactgga gattgaactg gaatgactgc gcaacctcca tccgcgtcca
      181 aaaacacacg ccctcgcgat acttctcgcc cggaatggga ttgtcttcgg tcgtgaaata
      241 cgtccgcagg ccgtggcagt gaaaacactt ctcgaacacc acgaagccaa actggatgtc
      301 tttcatcttc catagatgct ggcagtcgta cataacgctc cctatcgttc gagacgcctc
      361 gggacagcac cccgcaatgc ctgccggtag aggaaggtaa gcgaaatgcc cctaacactc
      421 tgctgatgca ccttgcctgc gatgatgcca aacccccccg gccaacctct cccggggggc
      481 aattctccta acccgtctga tgtgaagatg atatctatca gtagtatatg tcccccccgc
      541 gcgaaaaggc aagggaaatc tctccgtctt tatctgattt agcgacaagt ttgcccagat
      601 tcaacgctac aaacacgata aaagtcgtat tctttttcct gcgactccga tcacgacgcc
      661 caatcgcgcg ccaaaaaaaa accgctgtcg tcacgggacg acagcggctc caaaaactcg
      721 ccggaccacc cggcgactgt tcccaccgag cctacttcag aagcagcatt ttgcgcgaca
      781 ccacaccctc atccgtcgtg atgcgataca ggtacacgcc gctggccgcc ggtcggccgg
      841 ccccgtcgca gccgccccac gaaacggtgt gccggcccgc ctccagccgt ttatccaaaa
      901 gcacggcaac ctcttggccg agggtgttgt acaccgtgac ctgaacccgg gtcgctcccg
      961 tcagactgaa cgaaatgttg gtctccgggt tgaacgggtt cggataattg gtcacctcag
     1021 ataccttcgg catgtccagc gagagctgca cgatgagcgt gagcgccggg ctcaccccgg
     1081 ccacgctgat tctcaccgtg tccacatgga tgccgggcgg caatccggcc ggatcgaggt
     1141 agaggcacag gctgtcggcg gtcactcccg tcgggtccgg cagcgatgtg aatcggccga
     1201 ccgaatcggc cacccaggcc gtgaacggcg cgggcgcatt tgacgacgtg acgagcgcgc
     1261 acttcgtggt cgggggcagc atcccgtacg gcccgaaata gtgcaccgtc gcgggcgtga
     1321 ccgccgcggt gggcgcaccc ccgctgtcca ccgtcagcgt cagcatcacc ggcaccgtca
     1381 ccggcagcgc cacgccctcc accagacagg caatgctgtc gacatatgtc ccggccgaga
     1441 agggcgccgc cgggttgatg accagccgca ccgtatcact ggtgacgccc gccgagtccg
     1501 ccaccgacag ccaggccgag ccgtttgccg catacgcgac atacggggcc ggggcgttgg
     1561 tggaactgag aacgaagtgg cagtcgatga ggtcctgcag gccgaacggc gcggtgatgc
     1621 tcaccgtcgg cggcgccgcc caggcggagg gacccgggcc ggcggaatcg tccacgacgt
     1681 aaaacaccac cgggaccgcc gtcgccgccg ccacgccctc cacgctgcag tcgatcgtgt
     1741 cgacatagat cccctgcccg aggctcgccg cgctcgcgcc gaaacacacc gagtcattgg
     1801 tcatgcccga ggcgccgtag agcgtcagcc acggcgctcc gtggcggacg ctggccgtaa
     1861 acggggccgg cgcattgctc gaatagagca tgagacagcg cacctgcaag agctccgaat
     1921 actgcggcgc cgtgagactg
//
LOCUS       DAOOKS010000408         1938 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_74696_length_1938_cov_2.140733, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000408 DAOOKS010000000
VERSION     DAOOKS010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74696_length_1938_cov_2.140733"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="PK112_06880"
     CDS             complement(<1..180)
                     /locus_tag="PK112_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11809.1"
                     /translation="MSISHRYRLGLVLCALVTTAMAPSALAAEDKGKEAVCRKLAIPD
                     DPRVLPYRLEPIESQN"
     gene            424..>1938
                     /locus_tag="PK112_06885"
     CDS             424..>1938
                     /locus_tag="PK112_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11810.1"
                     /translation="MHLAFRSAGAAVLSALLVVLVAASAAAQREPADKDDFADLAAAA
                     PGPKPSIAAHNVGQLTVGVTNFGMIGIGRGKNQRIDCFTGRPVPKGEYPKGSRSVYLY
                     EGGLWVGGVVGGDSLVSTGAEFNNYNFEFWPTQPITTYSNVDPDDPNSALAKSEQDYY
                     ATYVDTMPVSSYDPIANRGHKPLGLRVEQASYAWSYPHTDDFIIITWQIKNISRKAIS
                     DVWVGIYWDADVHLQGIGDMLNRPDYGKPLTDGRDDLAGFMYTVPSADETCPGSDTIG
                     LAWTADNEGDYSSTTGLFAVPNVGAIRFLGPELTDRRYRVNYNWWVYNYSSGYDYGPQ
                     KRDRYRDLGNGPGTPYGDVNRYFMMSNGERDFDMVYTYGIGQNDPVWVPPSRYTSWAM
                     SRGGDVQQVLSVGPRYLAPGSEITVAMAFVCGSGLHYNPVNFRRKMLQAFEPDEYYAG
                     LDFSNLLANAQTAGRVYDIPGFDSNGDGYLGKMRECVVDSAFVDGRWVATALDTS"
BASE COUNT          338 a          666 c          574 g          360 t
ORIGIN      
        1 gttctgagac tcgattggct ccagccggta cggcagcacc cgcgggtcat ccggaatggc
       61 aagcttccgg caaaccgctt ccttcccctt gtcctccgcc gcaagcgccg acggcgccat
      121 ggcggtggtc acgagcgcac acaggactaa cccgaggcga tagcgatggg aaatcgacat
      181 ctcatctcat tcagataaac acccttgcct cggcaatata gccactgcca tccctcttgt
      241 cgagatcttc ttccgtagcc tggcgggcgg gcggccaggt cgcgggcggt cgcctgctaa
      301 ttcctgcgct cgtccgactc ggtgaatccg cttgtctggc cggaactttg cccatatctt
      361 cgcgtaagtg ctttcgatat ttgcgtctct gcgtgactac tttgtgagga cggaatcgtc
      421 tctatgcact tagcctttcg ttccgccggc gccgccgtgc tctccgcgct gttggtcgtt
      481 ttggtggccg cctcggccgc cgcccagcgg gagcctgccg acaaggatga tttcgcggac
      541 ttggcagccg ccgcgcccgg ccccaagccc agcatcgccg cccacaacgt cggccaactc
      601 accgtgggcg tcaccaattt cggcatgatc ggcatcgggc ggggcaagaa ccagcgcatc
      661 gactgtttca ccggacggcc agtcccgaag ggggagtacc ccaagggctc gcgctcggtg
      721 tacctctatg agggagggct ctgggtgggc ggcgtggtgg gcggcgacag cctggtctcc
      781 accggtgccg agttcaacaa ctacaatttc gagttctggc ccactcagcc catcaccacc
      841 tactccaacg tcgaccctga tgatcccaac tccgccctgg ccaaatccga gcaggactac
      901 tacgcgacct atgtcgatac catgccggtg agttcgtacg atccgatcgc caaccgcggg
      961 cacaagccgc tcgggcttcg ggtcgaacag gcctcctacg cctggtccta cccgcacacc
     1021 gacgatttca tcatcatcac ctggcagatc aagaacattt cgcggaaggc catttcagac
     1081 gtgtgggtgg gcatctactg ggatgccgat gtgcacctgc aggggatcgg cgacatgctc
     1141 aaccgccccg actacgggaa gccgctcacg gacgggcgcg acgacctcgc ggggttcatg
     1201 tacaccgtgc cgtcggccga tgagacctgc cccggctccg ataccatcgg cctcgcctgg
     1261 accgccgaca acgaaggcga ctactccagc acgaccgggc tgttcgcggt tcccaacgtg
     1321 ggggccatcc gctttctcgg ccccgagctg accgatcgcc gctaccgtgt gaactacaac
     1381 tggtgggtct acaactacag ttccggctac gactacggtc cgcagaagcg ggaccgctac
     1441 cgcgacctcg gcaacggacc ggggaccccg tacggcgacg tcaaccgcta cttcatgatg
     1501 agcaatggcg agcgggattt cgacatggtc tacacgtacg gcatcggcca gaacgacccg
     1561 gtgtgggttc ccccgagccg ctacacgtcc tgggcgatgt cccggggcgg cgacgtgcag
     1621 caggtgctct cggtcggccc gcgctacctc gcccccggct ccgaaatcac ggtcgccatg
     1681 gccttcgtct gcggctccgg cctgcactac aacccggtca acttccgccg caagatgctc
     1741 caggcgttcg agccggacga gtactacgcc gggcttgact tctccaatct cctggccaat
     1801 gcccagaccg ccggccgcgt ctatgacatc cccggtttcg acagcaacgg cgacggttac
     1861 ctggggaaga tgcgcgagtg cgtggtcgat tcggcgtttg tcgacggccg ctgggtggcc
     1921 acggcgctcg acacgagc
//
LOCUS       DAOOKS010000409         1937 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_74746_length_1937_cov_3.131243, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000409 DAOOKS010000000
VERSION     DAOOKS010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74746_length_1937_cov_3.131243"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..449
                     /locus_tag="PK112_06890"
     CDS             159..449
                     /locus_tag="PK112_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11811.1"
                     /translation="MSEMREKPEGSRPPAPEAAARTPRNHTIHVFVAHDLKARLEALA
                     ARYDRTTADMVRAVLRIGIPMMEGLSEAEEVMVKEYIELFRNLRQVKSLRDI"
     gene            562..>1937
                     /locus_tag="PK112_06895"
     CDS             562..>1937
                     /locus_tag="PK112_06895"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HPC11812.1"
                     /translation="MSERSLGRRAALTALCLALLAGCGSDPAPRPADQVEAALAAAGA
                     NRGELEKVLAHFDGKGDTLMLDAARYLIGNMEGHSYVTYALIDSLGDTIPWDAARFPD
                     YGALQAAYGELEKARPGLDFKSAVAARDLETVTADYLIENVELAFAAWRTKPWARKLS
                     YEEFREGVLPYRGSNEPLEPWRAELMARYADLAGKMTDSTDPVEAARLINDDIKSWFR
                     FDERWYFHPTDQGLAEMKTTGRGRCEDMTNLTIYAMRANGLAVTSDYTPYWANAGNNH
                     AWNALILPGGKAIPFMGAEADPGEYNLIRRAAKVYRKSFARQPRNLAFVKEENRKVPG
                     WLAGKSYRDVTAAYGEVCDVAVTFAEAPADSVRFAYLCVFNDGEWKPIQWAEIAGRRA
                     VFAAMAPEVVYLPGFYLNEEIVPAGEPFRFLAGQYVEILLKDGRRRAFSLANAPHDDS
                     RLQLHIRR"
BASE COUNT          368 a          642 c          655 g          272 t
ORIGIN      
        1 ctgcgcgagc ggttcgggct gccgttgcgc tcgccgcaga caatcctgag ccacgtcaaa
       61 ttccacccga aaccgccgga ccgccccgcg gccgacagtg gcacgcagga taccccgccg
      121 accccggaca tgaaccaacc aacggatgga gcacacagat gagcgaaatg cgggagaaac
      181 ccgagggcag ccgaccgccg gcgccggaag cggcggcgcg gacgccgcga aaccacacca
      241 tccacgtgtt cgtggcccac gacctcaaag cgcggctcga agcgctggcg gccaggtacg
      301 accggaccac ggccgacatg gtgcgggcgg tgctgcggat cggcatcccc atgatggaag
      361 gcctgtcgga ggccgaggag gtgatggtga aggagtatat cgagttgttc cgcaacctcc
      421 ggcaggtgaa gtcgctgcgg gatatctaag gccgagccgg gcgcgcacgt ttcagctttt
      481 ccgcggggcg tgaaatcgct atactggcgg cgtgattgga actgctcgac gagaacatcg
      541 cgagaaccgg agagtgcagc gatgagcgaa cgatccctgg gccggagggc ggctttgacc
      601 gccctgtgtc tggcgttgct ggcgggttgc gggagcgacc cggcgccgcg cccggccgat
      661 caggtggagg cggcgctggc cgcggccggc gccaaccgcg gcgaactgga aaaagtgctc
      721 gcccattttg acggcaaggg ggacacgttg atgctcgacg ccgcccggta tctgatcggc
      781 aacatggaag ggcacagcta cgtcacctac gccctcatcg acagcctcgg cgacaccatt
      841 ccctgggatg ccgcgcggtt tccggactac ggcgcgctac aggcggcgta cggggagctg
      901 gagaaggccc gccccggtct ggatttcaaa agcgcggtgg cggcgcggga cctcgagacg
      961 gtcacggccg actacctcat cgagaatgtc gagctggcgt tcgccgcgtg gcggaccaaa
     1021 ccgtgggcga ggaagctctc ctacgaggag ttccgcgaag gggtgctgcc gtaccggggg
     1081 agcaacgagc cgctcgaacc gtggcgggcg gagctgatgg cccgctacgc cgacctcgcg
     1141 ggcaaaatga cagactcaac cgacccggtg gaagcggcgc ggctgatcaa cgacgacatc
     1201 aagagctggt tccgtttcga cgagcgctgg tatttccacc ccaccgacca ggggctggcc
     1261 gaaatgaaaa cgaccggccg cggccgctgc gaggacatga ccaacctcac gatctatgcc
     1321 atgcgggcca acggactcgc cgtgacctcg gactacaccc cctactgggc gaacgcgggc
     1381 aacaaccacg cctggaacgc cctcatcctg ccgggcggga aagccattcc gttcatgggc
     1441 gccgaggccg accccggcga gtacaacttg atccgccgag cggccaaagt gtaccggaaa
     1501 tcgttcgccc gacagccgcg gaacctcgcc ttcgtcaagg aagaaaaccg caaagtgccc
     1561 ggctggctgg ccggcaagag ctaccgggac gtcaccgcgg cctacgggga ggtgtgcgat
     1621 gtggcggtga cgttcgccga ggcccccgcg gattcggtcc gcttcgccta cctgtgcgtg
     1681 ttcaacgacg gcgagtggaa accgatccag tgggccgaga tcgccggccg acgggcggtc
     1741 ttcgccgcca tggcccccga ggtcgtctac ctccccgggt tctacctcaa cgaagagata
     1801 gtgccggccg gagagccgtt ccgtttcctg gccggccagt acgtcgagat cctgctcaag
     1861 gacggccgcc gccgcgcctt ttcgctggcc aacgccccgc acgacgacag ccgcctgcag
     1921 ctgcacatcc gccgcat
//
LOCUS       DAOOKS010000410         1937 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_74755_length_1937_cov_2.778427, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000410 DAOOKS010000000
VERSION     DAOOKS010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74755_length_1937_cov_2.778427"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            367..>1937
                     /locus_tag="PK112_06900"
     CDS             367..>1937
                     /locus_tag="PK112_06900"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:NF015290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class II"
                     /protein_id="HPC11813.1"
                     /translation="MAKTVTNSELRFDVGANPIRYCGMQKVYPFSRDRVVEINLHHQH
                     VLEMYDLELVGGTAICLADRLNERSNLGALRNRKLREAAVASAALSAVAVSLHGRGSL
                     AALPKDKQTKEVANELKRANDRTSAQVMAEVLQTTTETLTIGEEVLIESTITEGVRAK
                     PGKEVGGNPTIAVGALFGKKEHRARYGLDLGKDVTLLSMGNDVIDGTTKSVKGLHSSM
                     TCMFLIESQVKRHLPDIYVQRWMGGAHFDEFNPREGTLLDAARVIADSYGFADLSELS
                     AFFIDRSRHYPAWDTLGAAGIGIPYDADGDLFPALLLGLEGMRFPNGRHMNSMIGEIG
                     GSAEWVVGALPLVWRGGQALGMLTSHSSLSRRDLSPEDLWNSRFHYTEDELIMIQDAR
                     FQQKPYFSVKDIIENPHAGGISAFGAITDSVYLPFMKGVQFDPDKGKISVWTLVINSL
                     GLMEVWEMHFACRTSLAHTRALIASPKERLAGLRGKALEAAIGKMLDDPREERRYRIF
                     FNNEYYPALVPIREKV"
BASE COUNT          354 a          640 c          631 g          312 t
ORIGIN      
        1 gacctgtccg taaggcggtc gttgcgggta cgaacctgcc gaatacgatc agccgcctcc
       61 ccctggggag gcggcttctt ttttggccgg tcggacggca gcaggggcgg ccgaaattgc
      121 ttgccatatc tcctccggta gtgtatctca cgaagagaag ccgcggtgag cgcggcgccc
      181 tcgcgggagc gggcaggagg atgcccgccg acggatcgcg ggcgcgggcg atcaggcggc
      241 ggggagaagc acgaagcgaa aaatggcctc tcccggccct tcgcgggcgt tgcgccggca
      301 ttgtggtctc cggtcggccg cggcggggcg gccgggcacg ggaccggtaa gggaaacgag
      361 ttcagtatgg ctaagacggt gaccaacagc gaactgcgct ttgatgtcgg cgccaatccg
      421 atccgctact gcggcatgca gaaagtctat ccgttcagtc gcgaccgggt ggtcgaaatc
      481 aacctgcacc accagcacgt tctggagatg tacgatctcg agctggtcgg cgggacggcg
      541 atttgcctgg ccgaccgcct caacgagcgc tccaatctcg gggcgctgcg caatcgcaaa
      601 ctgcgcgaag cggccgtggc ctcggcggcc ctctcggccg tggccgtcag tctgcacgga
      661 cgcgggagcc tcgcggccct gcccaaagac aagcagacca aagaggtcgc caacgagctc
      721 aagcgcgcca acgaccgcac ctcggcccag gtcatggccg aggtcctcca gacgaccacc
      781 gaaaccctca cgatcggcga ggaagtcctc atcgagtcga ccattaccga aggtgtccgc
      841 gccaaaccgg gtaaagaagt cggcggcaac ccgaccatcg ccgtcggcgc cctcttcggc
      901 aaaaaggagc accgcgcccg ctacggcctc gacctgggca aggatgtgac gctgctctcc
      961 atgggcaacg acgtgatcga cgggaccacc aagtcggtca agggtctgca ttcgtcgatg
     1021 acctgcatgt tcctcatcga gtcgcaggtg aagcggcacc tccccgatat ctacgtgcag
     1081 cggtggatgg gcggggcgca cttcgacgag ttcaacccgc gcgaggggac gctcctggat
     1141 gccgcccgcg tgattgccga cagctacggc tttgccgatc tcagcgaact cagcgccttc
     1201 ttcatcgacc gctcccgcca ctacccggcg tgggataccc tcggcgcggc cgggatcggc
     1261 attccgtacg acgccgacgg cgacctgttc ccggccctgc tgctcggact ggaggggatg
     1321 cgcttcccca acgggcggca catgaattcg atgatcgggg aaatcggcgg gtcggccgag
     1381 tgggtggtcg gcgcgctgcc gctcgtctgg cgcggcggcc aggccctcgg catgctcacc
     1441 agccactcct ccctctcccg ccgcgacctc tcgccggaag atctctggaa cagccgcttc
     1501 cactacaccg aggacgagct catcatgatc caggatgccc gcttccagca gaagccgtat
     1561 ttctcggtga aggacatcat cgagaacccc cacgccggcg gcatcagcgc cttcggcgcc
     1621 atcaccgaca gcgtctacct cccgttcatg aagggggtgc agttcgatcc ggacaagggg
     1681 aagatatcgg tgtggacgct ggtgatcaat tcgctcgggc tgatggaagt gtgggagatg
     1741 cacttcgcct gccgtacctc gctcgcccac accagggctt tgatcgcgtc gcccaaggag
     1801 cggctggccg ggctgcgcgg gaaagcgctc gaggcggcca tcggcaagat gctcgacgac
     1861 ccgcgcgagg aacggcgcta ccggatcttt ttcaacaacg agtactaccc ggcgctcgtg
     1921 ccgatccgcg agaaagt
//
LOCUS       DAOOKS010000411         1936 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_74806_length_1936_cov_3.208400, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000411 DAOOKS010000000
VERSION     DAOOKS010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74806_length_1936_cov_3.208400"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1288
                     /locus_tag="PK112_06905"
     CDS             <1..1288
                     /locus_tag="PK112_06905"
                     /inference="COORDINATES: protein motif:HMM:NF021387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HPC11814.1"
                     /translation="VMTGRIPAGSRPVALGKSLRMQQVALHYGRGELVLAIPDEYAIT
                     AFAPRPADEPVTYERFRAEFIGRGGLAAIRGEPVVVVNDGFRRTPTALVLEWLDRIDS
                     GLLDRASFLAATGTHGTPSEEDWGKIFGRFRERVRARTAVHDCRDAGSMTRLGEDPLG
                     GEVYVNRAVLGTRPVVVIGSVEPHYFAGYTGGRKGIFPGMVDLATTERNHNLARAMAA
                     APLRLAGNPVAEHLQALMDRIDGARFFSIQAVLDAAQRVAGVFCGGMTESFQRATVLA
                     DQIYAFRGAGPFGTVLAELRPPLDRNLYQLQKAVENCQAAVADGGALVVVSACAEGMG
                     SPHFADLATRWDPGGNAPRDGGPATFGSHKLSRVVAIARRIAVRVHTEMKDDEVRRVF
                     YEPLDNVANFLYLRRDACENNNLAVVYDAGHTVLTA"
     gene            1322..>1936
                     /locus_tag="PK112_06910"
     CDS             1322..>1936
                     /locus_tag="PK112_06910"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF004863.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HPC11815.1"
                     /translation="MNRKIAVVGAGNVGASCAMYLAEANLADVAMVDIIEGLPQGKGL
                     DLSQAGPVRGYNAMIDGSNDMAAVKGADIVVMTAGLARKPGMTREDLLTKNAEIVGGV
                     AKEIKKHAPKAYVIVVSNPLDLMTYHMMKVTGFPKERVIGQAGVLDSTRFRTFVAMEL
                     GVAMADVQAMVLGGHGDTMVPLSRYTTVAGIPIGELIPKERIKAI"
BASE COUNT          323 a          564 c          733 g          316 t
ORIGIN      
        1 tgtcatgacg ggccggatcc ccgccgggag ccggcctgtc gcattgggga agtccttgcg
       61 catgcagcag gtggcgcttc actacgggcg gggagaactg gtcctcgcca ttcccgacga
      121 gtacgcgatc accgccttcg ccccccggcc ggcggacgag ccggtcacct acgagcggtt
      181 ccgcgcggag ttcatcggac ggggcgggct tgcggcgatc cggggagagc cggtcgtggt
      241 ggtcaacgac ggttttcgcc gcacgcccac ggcgctcgtg ctcgagtggc tcgaccggat
      301 cgactccgga ctcctggacc gggcgtcatt tctggcggcc acgggaacgc acgggacgcc
      361 gagcgaggag gactggggga agattttcgg ccgcttccgg gagcgggtgc gggcgcggac
      421 ggccgtccac gactgccgcg acgccggatc gatgacgcgg ctgggagagg acccgctggg
      481 cggcgaggtc tatgtcaatc gggcggtgct cgggacgcgg ccggtggtgg tgatcggatc
      541 ggtcgagccg cactatttcg cgggctacac cggggggcgc aaggggattt tccccggcat
      601 ggtcgacctg gcgacgacgg agcggaacca caatttggcg cgggcgatgg cggcggcgcc
      661 gcttcgtctg gccgggaacc cggtggccga gcatttgcag gcgctcatgg accggatcga
      721 cggggcgagg tttttctcga ttcaggcggt gctcgatgcg gcgcagcggg ttgcgggcgt
      781 cttctgcgga ggaatgacgg agtcgttcca gcgggcgacg gtcctggccg accaaatcta
      841 cgctttccgc ggggcggggc cgttcgggac ggtgctggcg gaactccgtc cgcccctgga
      901 ccggaacctg taccagcttc agaaagcagt cgagaactgt caggcggcgg tggccgacgg
      961 gggggcgctg gtcgtggtct cggcctgcgc ggaggggatg gggtcgccgc attttgccga
     1021 tttggcgacg cgctgggatc cgggcggcaa tgccccgcgc gacggcggcc cggccacttt
     1081 cgggtcgcac aagctgtcgc gggtcgtggc gatcgcccgg cgcattgcgg tgcgcgtcca
     1141 cacggagatg aaagatgacg aagtgaggcg ggtgttttac gaaccgcttg acaacgtggc
     1201 gaactttttg tacttaaggc gcgacgcatg tgaaaataat aacttagcgg ttgtatatga
     1261 cgcgggccac acggttctca ctgcataacg ctccgcgccg tggcaacacc aggaggttcg
     1321 aatgaacaga aagatcgccg tagtaggagc cgggaatgtg ggtgcgtcgt gcgcgatgta
     1381 cctggccgag gcgaacctgg ccgatgtcgc gatggtcgac atcattgagg ggctgcccca
     1441 ggggaagggg ctcgacctga gccaggccgg gccggtgcgc gggtacaacg ccatgatcga
     1501 cgggtcgaac gacatggcgg ctgtcaaggg cgcggacatt gtagtgatga cggccgggct
     1561 ggcccgcaag ccgggcatga cgcgcgagga tctgctgacc aagaacgccg agatcgtggg
     1621 cggcgtggcc aaggagatca agaagcacgc gcccaaagcc tacgtgatcg tggtctccaa
     1681 cccgctcgac ctgatgacct accacatgat gaaagtgacg ggcttcccca aggagcgtgt
     1741 catcggccag gccggcgtgc tggattcgac acgcttccgc acattcgtgg ccatggagct
     1801 gggggtggcg atggccgacg tgcaggcgat ggtgctcggc gggcacggcg acacgatggt
     1861 gccgctgtcg cgctacacga ccgtggcggg catcccgatc ggcgagctga tcccgaaaga
     1921 gcggatcaag gcgatc
//
LOCUS       DAOOKS010000412         1936 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_74809_length_1936_cov_3.149389, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000412 DAOOKS010000000
VERSION     DAOOKS010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74809_length_1936_cov_3.149389"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1359
                     /gene="purH"
                     /locus_tag="PK112_06915"
     CDS             <1..1359
                     /gene="purH"
                     /locus_tag="PK112_06915"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF002049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HPC11816.1"
                     /translation="LHPRIHAGLLARRDVEAHLETMTQHEYKLIDLVVVNLYPFEQTV
                     AKAGATDDEVIENIDIGGPSMVRSAAKNHDGVAVVVDPGDYAELSAEMNAHDGGTTRA
                     FRRRCAAKAFALTARYDTAIAGYFAGAGQAGAKFAPQYRPSYSLRSALRYGENPHQEA
                     ALYARDGYGGPTLLEARILAGKELSYNNYGDLDSCLDMLLDFTEPFACVLKHANPCGA
                     ATGATIAEAYEAAYASDPLSAFGSIIGLNREVDLACARLLHETPFVECMIAPSFTDEA
                     LALLTKKKARRILALPSITLGWPRGQLADKPIRGGLLRQDTDDIETNPDALTVVTKRA
                     PTPEEIRSLLFAWKVVKHTKSNAIVLAKGTATVGIGMGQTSRVDSGFMAVKRAGERAR
                     GSVMASDAFFPMPDGVEVGTDAGVTAIIQPGGSKGDDEAIAAADKAGAAMVLTGHRHF
                     KH"
     gene            1591..>1936
                     /locus_tag="PK112_06920"
     CDS             1591..>1936
                     /locus_tag="PK112_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11817.1"
                     /translation="MIMQFGHYRYAATVTILLAAALIAIGFLLVDNGRLRQMLRDPAA
                     FVAAQQTGSEVPPLQAADVTGGICRLSYGPGEPATLLCWLTMRSSRGKDNIPFWNELA
                     HRFAGRNVRVIGM"
BASE COUNT          319 a          608 c          707 g          302 t
ORIGIN      
        1 ctccacccgc gcatccacgc cgggctgctc gcgcggcggg atgtggaagc gcacctggag
       61 acgatgaccc agcacgagta caaactgatc gatctcgtgg tcgtcaatct gtatccgttc
      121 gagcagacgg tggcgaaggc gggggccact gacgacgagg tgatcgagaa tatcgatatc
      181 gggggaccgt ccatggtccg atcggcggcg aagaaccacg acggggtggc ggtggtggtc
      241 gatcccggcg attacgccga gctgagtgcg gagatgaacg cgcacgacgg ggggacgacg
      301 cgggcgtttc gccgccggtg cgcggccaag gcgttcgcgc tgacggcgcg ctacgacacg
      361 gcgatcgccg gatatttcgc cggcgccggg caggcggggg cgaagttcgc gccgcagtac
      421 cgcccgagtt acagtctccg ctcggcgctg cggtacggcg agaacccgca ccaggaggcg
      481 gccctctacg cgcgcgacgg gtacgggggg ccgacgctgc tggaggcccg gatcctggcc
      541 ggaaaggagc tctcgtacaa caactacggc gacctcgact cctgcctgga catgctgctg
      601 gatttcacgg aaccgttcgc ctgcgtgttg aaacacgcca atccgtgcgg ggcggcgacc
      661 ggggcaacga tcgccgaggc ctacgaggcg gcctacgcga gcgacccgct gtcggcgttc
      721 gggtcgatca tcggtctcaa ccgggaggtt gacctcgcgt gcgcccggct cctccacgag
      781 acgccgtttg tcgagtgcat gatcgccccg tcgttcaccg acgaggcgct cgcgctcttg
      841 acgaagaaga aggcgcggcg gattctcgcg ctgccgtcga tcaccctcgg ctggccccgc
      901 gggcaactgg ccgacaagcc gatccggggc gggctgctgc ggcaggacac cgacgatatc
      961 gagaccaatc cggacgcgct cacggtcgtg accaaacgcg cgcccacgcc ggaggagatc
     1021 cggtcgctgc tgttcgcgtg gaaagtcgtg aaacacacca agtcaaacgc catcgtgctg
     1081 gcaaaaggaa cggcgacggt ggggatcggg atggggcaga cctcgcgggt ggattccggt
     1141 ttcatggcgg tgaagcgggc gggcgagcgg gcccggggct cggtgatggc ctcggatgcg
     1201 tttttcccga tgccggatgg cgtcgaggtg ggcaccgacg cgggggtgac cgcgatcatt
     1261 cagccgggcg gttcaaaggg ggacgacgag gcgatcgccg cggccgacaa agccggggcg
     1321 gcgatggtgc tgaccggaca ccggcatttc aagcattgag tccggcgccg gcggcgaccg
     1381 gcgcaccgcg tctgccaaac tacgagtcgg atgggggccg ggttccgcgg aacccggccc
     1441 gcgtttttcg ggagcggggc gggcaaaaac ttcctcttga cactgtttcg gcggccggga
     1501 catattggcc ccaagctttc gcattcgatc aagtcattaa catcgtcttc ggtgcggacc
     1561 gggcggccac cgggcggtcc ggcccggcac gtgattatgc aattcggcca ctatcggtac
     1621 gcggcgacgg tcaccatact gctcgcggcg gcgctcatcg cgatcggctt tctcctggtc
     1681 gacaacggcc ggctgcggca gatgcttcgg gatccggcgg cgttcgtggc cgcgcagcag
     1741 accggctcgg aggtgccgcc gcttcaggcg gccgatgtca ccggcggcat ctgccggctc
     1801 tcgtacgggc cgggagagcc ggccacgctg ctgtgctggc tcacgatgcg gtcgtcgcgc
     1861 ggaaaggaca atatcccctt ctggaacgag ctggcgcacc ggttcgccgg gcggaacgtg
     1921 cgcgtgatcg ggatgt
//
LOCUS       DAOOKS010000413         1935 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_74868_length_1935_cov_3.189894, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000413 DAOOKS010000000
VERSION     DAOOKS010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74868_length_1935_cov_3.189894"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1086
                     /locus_tag="PK112_06925"
     CDS             <1..1086
                     /locus_tag="PK112_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009196656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPC11818.1"
                     /translation="QAPTLEKLKAAIPPFMERAQTDSVFQVVDLNLRFNKPELTVGID
                     RDRARALGITVRDIAETLQLYFSGQRYGYFIMNGKQYPVIAQADRPNRDEPLDLSSIY
                     IRNNRGELIQLDNVVTLSYRSSPPQLYRYNRYVAATVSAAPAPGVALGTAIDEMQGIA
                     DSVLDESFSTSLAGTAKEYAESSNTLYFAFVLALVLVYLILSAQFESFRDPLIIMFTV
                     PLALAGAILSLWLFGQTFNIFSQIGIIALVGIVTKNGILIVEFANQRRAQGLGIREAV
                     IDAAAQRFRPILMTSLATVFGVLPIALALGAASKSRASMGIVIIGGLLFSLVLTLFVI
                     PALYTYLSSRRRHEVEATDAAAVAEAV"
     gene            1486..1851
                     /gene="ssrA"
                     /locus_tag="PK112_06930"
     tmRNA           1486..1851
                     /gene="ssrA"
                     /locus_tag="PK112_06930"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT          327 a          575 c          651 g          382 t
ORIGIN      
        1 caggcgccga cgctggagaa gctcaaggcg gcgatcccgc cgttcatgga gcgggcgcag
       61 accgattccg ttttccaggt ggtcgatctc aacctccgct tcaacaagcc ggagctgacc
      121 gtcgggatcg accgtgaccg cgcccgggcc ctcgggatta cggtgcgcga catcgccgag
      181 accctccagc tgtatttcag cggccagcgc tacggctact tcatcatgaa cggcaagcag
      241 tacccggtga tcgcgcaggc cgaccggccc aaccgcgatg aacccctgga cctcagctcc
      301 atctacatcc gcaacaaccg gggggaactg atccaactgg acaacgtggt gacgctctcc
      361 taccggagca gcccgccgca gttgtaccgg tacaaccggt atgtcgcggc caccgtgagc
      421 gcagccccgg cgcccggcgt agcgctcggg acggctatcg acgaaatgca ggggatcgcc
      481 gacagcgtgc tggacgagtc gttttccacc agtctggccg ggacggccaa ggagtacgcc
      541 gagagctcga acacgctgta cttcgctttt gtgctcgccc tcgtcctggt gtacctgatt
      601 ctgtcggccc agtttgagag ttttcgcgat cccctgatca tcatgttcac ggtgccgctg
      661 gcgctggccg gggcgatcct ctcgctgtgg ctgttcgggc agaccttcaa catattcagc
      721 cagatcggca tcatcgcgct ggtcgggatc gtgaccaaga acgggatttt gatcgtcgaa
      781 ttcgccaacc agcggcgtgc ccagggactg gggattcggg aggcggtgat cgacgcggcg
      841 gcgcagcggt tccggccgat cctgatgacg agtctggcga ccgtgttcgg ggtgctgccg
      901 atcgcgctgg ccctcggggc ggcctccaag agccgggcct cgatgggtat cgtgattatt
      961 ggcggacttc tgttttcgct ggtgctgacg ctcttcgtca tccctgcgct gtacacctac
     1021 ctgtcctcgc ggcggcggca cgaggtcgaa gcgaccgacg ccgcggcggt ggcggaggcc
     1081 gtgtaggggg cggggacgcg gccggcacgg cgggatcgtg cgtcagtccg gtggagccgc
     1141 ttgcagcgca agggctgcaa gaggatcccg gacggcggcc gcccggcgag ccggcgggat
     1201 cgcgaaccga cggtttgaca gcaccggttg actgcgcgct ggcgatccgg cgggactgtt
     1261 ggattgcggg ccgacggctt ggccgtccga cctgatcgtg ggcctgcggc tccggatcgg
     1321 cggggcggag tggaacatcg gcgggggatt ttcgttacaa cagtgcgggc attgcgtatt
     1381 ccggtcgata gcgtatattg gggttcggcg atgacccgca gtgttctttg acagctcaag
     1441 aggcggcggc ttctcttgag cctgcagaaa cgcttgtcac gatatggggg tgaccaggtt
     1501 tcgacgggtt tagcgaggag ttgtcggcgt gccggggatg tctggagccc cgttaccaat
     1561 tcaggcaaac cgataaatgg cgaagctaat ttcgctatgg ctgcctaaga gagaatctta
     1621 ggttcagccc gtcgcgcccg attttgtctt tcgggtcggg agccgcggcg tcgatgacgg
     1681 aagggtcggt cggctgatgt ctctaaagcg ggcttaaagc tcagaggctg gcttcggcgg
     1741 tggcctgtcg atcggcagcg gtcggagcga gaatggtaga tcgactacgc acgtagttcg
     1801 gcggcttggc gttagattcg gacgggggtt cgattccccc cacctccaaa tcattgtccc
     1861 acactaagtt actatgacac aaggggtggg atgatttgcc tattcccccc acccctctta
     1921 tcccccaacg aatta
//
LOCUS       DAOOKS010000414         1935 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_74876_length_1935_cov_2.782979, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000414 DAOOKS010000000
VERSION     DAOOKS010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74876_length_1935_cov_2.782979"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..883)
                     /locus_tag="PK112_06935"
     CDS             complement(<1..883)
                     /locus_tag="PK112_06935"
                     /inference="COORDINATES: protein motif:HMM:NF024876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase MA family metallohydrolase"
                     /protein_id="HPC11819.1"
                     /translation="MRKSHILAPLLALAAALVVSGTAPAQETYFGKNKVRYKNFDWEY
                     IQTRHFDIHFYEGAYPTAKFAAAVLESAYVEITQELGYHLQEPVPIFVYNSHNDFQQT
                     NIISSLIPEGVGGFTEAFKNRIVIPFTGSYEDFRHVLHHELTHAVIYDLLFGKSLSSL
                     ISRNRLFNLPLWFAEGYAEYSSRHGWDYWADMVVRDATINGYLAPPDYVGGYLAYKQG
                     QAMIKYIADKYGEDKLGEILRKGKVYLTMDRALKEAIGIDQEKLWKEFSLEMKRHYWP
                     EIASRKNADEIGRQLTKA"
     gene            complement(974..>1935)
                     /gene="murA"
                     /locus_tag="PK112_06940"
     CDS             complement(974..>1935)
                     /gene="murA"
                     /locus_tag="PK112_06940"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF006873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HPC11820.1"
                     /translation="GPILARLGRARVSLPGGCVLGARPVDFHIKGFRALGAKVTEEAG
                     YVIAEAKPLAGGSVYFDRPSHTGTENLLFGTVFAKRRTFIANAACDPEVVDVANFLNK
                     AGARITGAGTPNVVIEPVRRLKAVEYTVSGDRLVAGTFLMAGAITGGRVKVAGCDPEH
                     LRVVLHKLGEMGCTSEATRTTVTVAGPKRLQPVRVTTFPYPGFPTDLQACIMAMACIA
                     EGTSHIRETVFEDRFSHTMEMRRLGADISVSSDEAVINGVSALRGATVMASDIRAGAG
                     IVLAALAAAGRSEILRVYHIDRGYNRIEEKLGALGADITREAE"
BASE COUNT          330 a          626 c          619 g          360 t
ORIGIN      
        1 gcgccttggt gagctgccgc ccgatctcat cggcattttt gcgggacgca atctccggcc
       61 agtagtgccg cttcatctcg agcgaaaact ctttccacag tttctcctgg tcaatcccga
      121 tcgcctcttt gagcgcccgg tccatggtca ggtagacctt ccccttgcgc aggatctccc
      181 ccagcttgtc ctcgccgtat ttgtcggcga tgtacttgat catggcctgc ccctgcttgt
      241 aggcaaggta gccgccgaca tagtcgggcg gggccaggta gccgttgatc gtcgcgtcgc
      301 gcaccaccat gtcggcccag tagtcccagc cgtgccgcga ggagtactcc gcatagccct
      361 cggcgaacca gagcgggaga ttgaacagcc ggttgcgcga gatgagcgag gacagggatt
      421 tgccgaaaag gagatcgtag atgacggcgt gggtcagctc gtggtggagc acgtgccgga
      481 aatcctcgta ggatccggtg aacgggatca cgatgcggtt cttgaaggcc tcggtgaaac
      541 cgcccacccc ttcgggaatg agactggaga tgatgttggt ctgctggaaa tcgttgtggg
      601 agttgtacac gaaaatcggc accggctcct ggaggtggta gccgagctcc tgggtgatct
      661 ccacgtaggc cgactcgagc acggcggcgg cgaacttggc ggtcggatac gccccttcat
      721 agaaatggat atcgaagtgg cgggtctgga tgtactccca gtcgaagttc ttgtaccgga
      781 ccttgttctt cccgaaatac gtttcctggg ccggggcggt cccggagacg accagggcgg
      841 cggccagggc gagcaacggg gcgagaatgt gggattttct catgtcgggc atcacctttt
      901 cggttttggg ccgccgcggc gccgccgggg gaaacgaccg gcccgcggtc cggtcacggg
      961 agaaccgggc gtcttactcc gcttctctgg ttatatcggc gccaagagcg ccgagctttt
     1021 cctctatccg gttatacccc cggtcgatat gatacacccg gagtatctcc gaccgaccgg
     1081 cggccgccag ggccgccaac acgattcccg cccccgcccg aatgtccgac gccatcacgg
     1141 tcgccccgcg cagcgcggaa acgccgttga tcaccgcctc gtcgctggac acgctgatgt
     1201 ccgccccgag gcggcgcatc tccatcgtat ggctgaacct gtcttcaaaa acagtttccc
     1261 ggatatgcga ggttccctcg gcaatacagg ccatcgccat aatgcaggcc tgcaggtcgg
     1321 tcgggaaacc gggataggga aaagtcgtca cccgcaccgg ctgaagccgt ttcgggccgg
     1381 cgacggtcac ggtggtgcgt gtggcctccg atgtgcagcc catctccccg agcttgtgga
     1441 gcaccacccg gaggtgctcc gggtcgcacc cggcgacttt cacccgccca ccggtgatcg
     1501 cccccgccat cagaaacgtg ccggcgacca gccggtctcc ggacacggtg tactccaccg
     1561 ccttcagccg cctgaccggc tcgatcacca cgttcggcgt gcccgcgccc gtgattcggg
     1621 ctcccgcctt gttcagaaaa ttggcgacgt cgaccacctc cgggtcgcag gcggcgttgg
     1681 cgatgaaggt ccgccgtttt gcgaagacgg tgccgaagag gaggttctcg gtgccggtgt
     1741 gggagggccg gtcgaaatac accgaccccc ccgcgagcgg cttggcctcg gcgatgacgt
     1801 acccggcctc ctcggtgact ttggccccga gtgcccggaa ccctttgatg tggaaatcca
     1861 ccggtcgggc gcccagcacg cagccgcccg gcagcgacac gcgggcccgg ccgaggcggg
     1921 cgagaatcgg cccga
//
LOCUS       DAOOKS010000415         1934 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_74938_length_1934_cov_2.769558, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000415 DAOOKS010000000
VERSION     DAOOKS010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74938_length_1934_cov_2.769558"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="PK112_06945"
     CDS             <1..180
                     /locus_tag="PK112_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017286121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HPC11821.1"
                     /translation="WEPAKWTAKLRATKTDDHVLLLKTNMGAGHGGASGRYDYLKEVA
                     LEYAFILDQLGLTGP"
     gene            complement(534..893)
                     /locus_tag="PK112_06950"
     CDS             complement(534..893)
                     /locus_tag="PK112_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11822.1"
                     /translation="MWTDVLIPLVIFGTGVVIVKLVLDYKLRRQILEKGPVDDKAQRF
                     LLREAELARLSSLKWGMVLVGLGIALLVGYMNPDMFDDGGVFGLMLVLAGIGFLIYFA
                     VAQRHLGRLERPPQGSV"
     gene            1025..1624
                     /locus_tag="PK112_06955"
     CDS             1025..1624
                     /locus_tag="PK112_06955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPC11823.1"
                     /translation="MEQAAPDNRSLIEEILRGSQAAFRTFVSRHQRLVSHIVFRMVAN
                     AADREDVCQDVFLKVFHNLGSFRHDARVSTWVARIAYNACIDHLQRRKIPLLEDLAGE
                     DGSSPLETIADPAEGADAAAETRDLFARVQAEMAYLPPQYRAILTLYHLDEMTYEEIV
                     EITGLPMGTVKNYLFRARRQLRDRLLTKYRTEDIWLTGT"
     gene            1606..>1934
                     /locus_tag="PK112_06960"
     CDS             1606..>1934
                     /locus_tag="PK112_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11824.1"
                     /translation="MVDRHMTDDEMQAYLETGTSALDQVFESHLAECRDCRERVESYR
                     ALFAALGRDPGWVLSPGFVGAVEARLAADRAAGAKRRWYHALWWLGGMAAAFGATAYF
                     MGLGTFTG"
BASE COUNT          364 a          596 c          639 g          335 t
ORIGIN      
        1 tgggagccgg ccaagtggac ggcgaaactc cgcgccacca agaccgatga ccacgttctg
       61 ctgctcaaga ctaacatggg ggcggggcac ggtggggcct ccggccgcta tgattacctg
      121 aaagaggttg ccctggagta cgccttcatt ctcgaccagc tcggcctgac cgggccgtga
      181 gcggaggaag cggcctgggc gcaaaccgga gggccgggga cccgaccggg gcgccccggc
      241 gcagtcgtca ggcaaacaag aaactcgccc gggcggggac accgcgggcg agtttcttta
      301 ggggaggtct catgaagcga cggcagtata ataggcctta tggatcaagt caatcttttt
      361 ttgcaccaaa acgcgacttt ttttatcccc tcgtcgcgcc ggtcccggac agcaaaccaa
      421 ggcacgtatc ctattgcgtc acaaactatt gcgttgtcgt gtggccgtcg aaagaaagga
      481 aaacggcgct ccccaggcgg gacagcgccg ttaaggtcca tcggagaatg acctcagact
      541 gatccctgcg gcggccgctc gagccgcccg aggtgccgct gggcgacggc gaagtagatg
      601 aggaatccga tcccggccag gacaagcatc agcccgaaca cgcccccatc gtcaaacatg
      661 tccggattca tgtagccgac cagcagcgca atcccgagcc cgaccagcac catgccccac
      721 ttcagcgacg acagccgggc cagttccgcc tcccggagaa ggaaacgctg cgccttgtca
      781 tccaccggtc ccttctcgag gatctggcgc cggagcttgt agtccagcac cagtttgacg
      841 atgacgacgc cggtcccgaa aatcaccagc gggatcaaga catcggtcca cataaatact
      901 ctccttgcat cgcgtttccg tcctgtctga ccgattcgac cgcccggcgc ggcgttttgt
      961 tgcaccggag aatgcatttt ctttgatgca acaccgcggc cgcctatact gtctttaggt
     1021 atgtatggag caggctgcgc cggacaaccg gtccttaatt gaggaaatcc tccgcggcag
     1081 ccaggcggcg ttccggacgt tcgtgagccg gcaccagcgg ctggtcagcc acattgtttt
     1141 ccggatggtg gcgaatgccg ccgaccgcga ggatgtctgc caagacgtgt tcctcaaggt
     1201 tttccacaac ctcggcagct tccgccatga cgccagggtg tcgacgtggg tggcccggat
     1261 cgcctacaac gcgtgcatcg accacctgca gaggcggaag atcccgctgc tggaagatct
     1321 ggccggcgag gacggatcgt cgccgctgga gacgatcgcc gatccggcgg aaggggcgga
     1381 tgcggcggct gagacgcgcg acctgtttgc ccgggtgcag gcggagatgg cgtatctgcc
     1441 gccgcagtat cgggcgatcc tcacgctgta ccatctcgac gagatgacgt acgaggagat
     1501 cgtggagatc accggcctgc ccatgggcac ggtgaagaac tacctgttcc gggcgcgccg
     1561 gcagttgcgc gaccggctgt tgacgaaata ccgaacggag gacatatggt tgacaggcac
     1621 atgaccgacg acgagatgca ggcgtatctg gagaccggga cgtcggcgct cgaccaggtg
     1681 ttcgagtccc accttgccga gtgccgcgac tgccgggagc gggtggagag ctatcgggcc
     1741 ctctttgcgg cgctcggccg ggatcccggc tgggtgttgt cgccggggtt cgtcggggcg
     1801 gtcgaggcgc ggctggccgc ggaccgggct gccggcgcca agcggcgctg gtaccacgcc
     1861 ctctggtggc tgggagggat ggcggcggcg ttcggggcca cggcgtactt catggggctc
     1921 ggcactttca cagg
//
LOCUS       DAOOKS010000416         1930 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_75183_length_1930_cov_2.667200, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000416 DAOOKS010000000
VERSION     DAOOKS010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75183_length_1930_cov_2.667200"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(178..1056)
                     /locus_tag="PK112_06965"
     CDS             complement(178..1056)
                     /locus_tag="PK112_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HPC11825.1"
                     /translation="MNTLKVTFMMLALMLLFMWVGYLVGGQSGLIIAFVFAAGMNFVS
                     YWFSDKIVLAMYRARQVSESDHPRLFRVVKHIAAAARMPTPKVYIVPSKGPNAFATGR
                     SPEHAAVAVTEGLLELLDENELEGVVGHEFAHIVNRDMLVGTIAATFAGAIGMLASMA
                     RWGAIFGGYGGRDNERSGGAIGLLVAAIVAPLAAMLIQMAISRAREYKADEAGGGFTG
                     KYLPLASALDKLHRAPVKMRLDDRPATAHLMIANPLSGKGLSSLFSTHPPAEKRIEKL
                     RQLAQQSAYKGYAGAR"
     gene            complement(1225..>1930)
                     /locus_tag="PK112_06970"
     CDS             complement(1225..>1930)
                     /locus_tag="PK112_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11826.1"
                     /translation="IPTGGETPAPDRGRPAAPVTPPLREADRRPSREPAPPVEPPAPR
                     EPARETAPDVPVPEIRRRPLPIEVRAAQRPDDRPGATPPAEAPAPESPVRAEAPRREF
                     APEPPPPPPIWRPAEPAEPKTDKSDDEFVPDAPATEPRLREILASLAASRERLDVESA
                     DAEGEDSDEKEGPGEPEPEFRPASFGRGRARHGLRPSDQTQPPEPETPEPTDRKDGYD
                     VSAVPSFGRARRKRMR"
BASE COUNT          301 a          624 c          686 g          319 t
ORIGIN      
        1 gactttctgt ttcaggcggc tcttccgcga ggcgatgacg gagaactcgg acagggcgaa
       61 aaacccgttg gccagcacga gaacgagaat agcgataatc tgcagccaga gggagtccac
      121 tagcggctcc ctccgcatct actgccgggc aacggatcgt cggcccggtc ggaggatcta
      181 tcgcgcgcct gcatatccct tgtaggcgga ttgctgggcc aactgccgga gtttctcaat
      241 ccgcttttcc gccggcgggt gggtcgagaa gagcgacgag aggcccttgc cggaaagcgg
      301 gttggcgatc atcaggtggg ccgtcgccgg acggtcatcg agccgcatct tgaccggcgc
      361 gcggtggagc ttgtccaggg ccgaggccag gggaaggtac ttgccggtga acccgccgcc
      421 ggcctcatcg gccttgtact cccgggcgcg cgagatcgcc atctggatga gcatggccgc
      481 gagcggggcg acgatggcgg ccacgaggag gccgatggcg ccgccgctgc gctcgttgtc
      541 gcggccgccg tagccgccaa aaatcgcgcc ccaccgggcc atcgaggcca gcatgccgat
      601 ggcgccggcg aaggtggcgg caatcgtgcc gacgagcatg tcgcggttta cgatgtgggc
      661 gaattcatgc cccaccacgc cttccagctc attctcatcc agcagctcca gcagcccctc
      721 ggtcaccgcg accgcggcgt gttcggggct ccgcccggtg gcaaaagcgt tgggtccttt
      781 gctggggacg atgtacactt tcggcgtagg catgcgggcg gcggccgcaa tgtgcttcac
      841 caccctgaac agccgggggt ggtcgctttc cgaaacctgg cgcgcacggt acatggccag
      901 cacgatcttg tccgaaaacc agtagctgac aaaattcata ccggcggcga agacaaaggc
      961 gatgatcagc ccggactgcc cgccgaccag atacccgacc cacatgaaca gaagcatgag
     1021 ggccagcatc atgaacgtga ctttcagggt gttcactatt tccgttacct cgctttcaat
     1081 ctaaacccgc tcgtgggggt cggtcaaatg atttcgttta cccccaatac cgtcggggcc
     1141 gggcgaaagt tccccggcgg gtctccgatc cggttcggca accatccgcg ggacaacatg
     1201 ttaaggcgaa tcggcgggag ccgctcaacg catccgcttc ctccgcgctc gcccgaacga
     1261 gggaaccgca ctcacgtcat agccatcctt gcggtccgtc ggttcgggag tctctggttc
     1321 cggcggctgc gtctggtcgg aggggcgaag tccatggcgc gcgcgcccgc gcccgaagga
     1381 cgccggccgg aattccggct ccggctcccc cggtccttcc ttctcgtcgg agtcctctcc
     1441 ctcggcgtcg gccgactcca catcgagccg ctcgcgcgag gcggccagac tggcgaggat
     1501 ctcgcgaagg cggggctccg tggcgggggc atcggggacg aactcatcgt cggacttgtc
     1561 ggtcttcggc tccgccggtt ctgcaggcct ccagatgggc ggagggggag gcggctccgg
     1621 ggcgaattcg cgccggggag cctcggcccg cacaggtgac tcgggcgcgg gcgcctcggc
     1681 gggaggggtt gcgccgggcc ggtcatcggg acgctgcgcg gcgcgcactt cgatcgggag
     1741 tggccggcgg cggatttcgg gtaccggaac atcgggggcg gtttcccggg caggctcgcg
     1801 aggtgcgggc ggctcgacgg gcggggccgg ttcgcggctt ggacgacggt cggcctcgcg
     1861 cagcgggggc gtcaccgggg cggcggggcg gccgcggtcg ggcgccggcg tctcaccgcc
     1921 ggtcggtatg
//
LOCUS       DAOOKS010000417         1928 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_75299_length_1928_cov_3.545115, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000417 DAOOKS010000000
VERSION     DAOOKS010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75299_length_1928_cov_3.545115"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1666)
                     /locus_tag="PK112_06975"
     CDS             complement(<1..1666)
                     /locus_tag="PK112_06975"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPC11827.1"
                     /translation="MKELAAYTLSLFAGLIMLGLNTGAAAAQPSLAPGLAELIDRGAP
                     PDSIVHVVVFLETERTAAASKASAAMLARGDQIRQVTSALKQARGRGDRAVAALLRQH
                     ARSTVARYWIAPAYAAAVPAAVLPELAAIDGVSLVVEDAPVSLIAPVEEKAPTAGLAA
                     TVAQPLVQLGVPALWQKGLTGRGRLICSFDTGVKYDHAALLPKWRGAHVPLSAAWFSK
                     VAPTSLPADAVGHGTHTMGIMVGSDGADTIGVAPDAEWITAGVIDQGRPLSTTIADIL
                     EAFQWALNPDGDTMTTDDVPDVILNSWGIPKGLFTPCDRTFAAAIENVEAAGVVTVFA
                     AGNEGPNAMTLRNPADMVLSPLSAFAVGAVDANNAVASFSSRGPASCDQVSVKPELVA
                     PGVSIRSSYKDGGYKVLTGTSMAAPFVAGLVALVRQYNPNVSAADIKSAFIAAARDLG
                     PLGEDNSYGYGIVDAARLLQYIPDPVAPDFSISRVAVSGDGLAVPGERFDLAITLRNP
                     AANIVRATATLAAPAASGVTVESGEVIYLFGGDLLSAVNGAPLTATF"
BASE COUNT          301 a          695 c          639 g          293 t
ORIGIN      
        1 tgaaggtcgc tgtcaacggc gccccgttga ccgcgctcag caggtcgccg ccgaacaggt
       61 agatcacttc ccccgactcg actgtgacgc ccgaggccgc cggggccgcg agagtggccg
      121 tcgcccggac aatattggcc gccgggttgc gcagcgtgat cgccaggtcg aagcgctcgc
      181 ccggcaccgc cagcccgtcg cccgaaaccg ccacccggct gatggagaaa tcgggcgcca
      241 ccggatccgg gatgtactgg agcagccgcg cggcatcgac gatcccgtag ccgtagctgt
      301 tgtcctctcc gagcggcccc aggtcgcggg cggccgcgat aaaggcgctc ttgatgtcgg
      361 cggccgacac gttgggatta tactggcgga cgagcgccac cagtccggcc acgaacggcg
      421 cggccatcga ggtgcccgtg agcaccttgt acccgccgtc tttgtacgac gaccggatac
      481 tcacgcccgg ggcgaccagc tccggcttca ccgacacctg gtcgcacgat gccggacccc
      541 ggctggagaa gctcgcgacc gcgttgttgg catcgacggc gccgaccgcg aaggcgctca
      601 gcggactgag caccatgtcg gccgggttgc gcagcgtcat ggcgttcggc ccttcgttgc
      661 cggccgcaaa caccgtcacc acgccggccg cctccacgtt ctcgatcgcc gcggcaaacg
      721 tccggtcgca cggcgtgaag agacctttcg ggatccccca gctgttcagg atcacgtcgg
      781 gcacatcgtc ggtcgtcatc gtgtccccgt ccggattgag cgcccactgg aaagcttcga
      841 gaatgtcggc gatggtggtc gagagcggcc gtccctggtc gatcacgccg gccgtgatcc
      901 actccgcgtc cggggccacg ccgatcgtgt ccgccccgtc ggagccgacc atgatgccca
      961 tcgtgtgggt accgtggccg accgcgtccg ccggcagact tgtcggcgcc accttggaga
     1021 accaggccgc gctcagcgga acgtgcgcgc cccgccactt cggcaggagc gccgcgtgat
     1081 cgtacttcac tcccgtatcg aacgagcaga tgagccgccc gcgccccgtc aaccccttct
     1141 gccacagtgc ggggactccg agctgcacaa gcggctgcgc aaccgtcgcc gccagaccgg
     1201 ccgtcggagc cttttcctcg accggcgcaa tcaggctcac cggcgcatct tcgaccacga
     1261 ggctcacgcc gtcgatcgcc gccagttccg ggaggaccgc cgccggcacc gctgccgcgt
     1321 aggccggtgc aatccagtaa cgggcgacgg tcgagcgggc gtgctgccgg agcagcgcgg
     1381 ccaccgcccg gtccccccgc ccccgcgcct gcttcagcgc cgatgtgacc tgccggattt
     1441 gatctccccg ggcaagcatc gcggccgacg ctttcgacgc ggcggcggtg cgttccgtct
     1501 cgaggaagac gaccacgtgc acgatgctgt ccggcggcgc tccgcggtcg atcagttcgg
     1561 cgagccccgg cgccagcgac ggctgggcgg cggccgcccc ggtgttcagc cccagcatga
     1621 tcagacccgc gaacagcgag agcgtgtagg ctgcaagttc cttcatgggt tctcctccgg
     1681 caaagtccgg ggacccttag agcaacggtt atgccgcccc ccggcttatg agggggccgc
     1741 aaccggctgc accgactcac cctcagggcg agctaatcgc tattaccgca acgactttcg
     1801 ggggaggcgc tggggtcgcg cgagcggggc gagcggttga ccacttgcac aggtgaaagg
     1861 ccggtgcagt ccgatgcaca ggcgggtgca cactcggcaa ctttttacag gctgattcca
     1921 gcggcggg
//
LOCUS       DAOOKS010000418         1918 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_75886_length_1918_cov_3.108427, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000418 DAOOKS010000000
VERSION     DAOOKS010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75886_length_1918_cov_3.108427"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="PK112_06980"
     CDS             <1..638
                     /locus_tag="PK112_06980"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HPC11828.1"
                     /translation="VRAEASLRQALEVRRLPFDVDEGGGAFYGPKIDIKIKDALNRSW
                     QCSTIQFDFNLPERFDMHYIDADGRPKRPFMIHRALLGSIERFFGVLIEHYAGKFPLW
                     LAPVQVKVLPITDSQNDYGRALADELKRHGIRAQVDDRSDKIGAKIRDAELMKVPYMF
                     VVGGREAENRNVAVRKHGVGDLGVRSLEEARDMLLAEIRTRGLAQSGAGAR"
     gene            663..1328
                     /gene="infC"
                     /locus_tag="PK112_06985"
     CDS             663..1328
                     /gene="infC"
                     /locus_tag="PK112_06985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HPC11829.1"
                     /translation="MRINRRIRVSPVRLIGPEGEQIGILPTKEALERAETLGLDLVEV
                     SPNSRPPVCRIMDFGKYKYELSKKDRQAKKRQHSFQLKEMRFRPKIDEHDFEFKTKHV
                     RNFLESGSKVRVYVMFRGREMAYQEYGRKVLDRVAEELKDIATVESAPKMEGRNMNML
                     VAPRPEVMKVLKQKAEQRERAELKERERAAEAGEAMPDEQPGELQESPADGGGDRTGE
                     NNE"
     gene            1350..1544
                     /gene="rpmI"
                     /locus_tag="PK112_06990"
     CDS             1350..1544
                     /gene="rpmI"
                     /locus_tag="PK112_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005842446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HPC11830.1"
                     /translation="MPKMRTQRAAAKRFKKTGTGKYKRKRAFKSHILTKKTPKRIRKL
                     RRPAILSHADQGRVKRMLPN"
     gene            1582..>1918
                     /gene="rplT"
                     /locus_tag="PK112_06995"
     CDS             1582..>1918
                     /gene="rplT"
                     /locus_tag="PK112_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007151143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HPC11831.1"
                     /translation="MARAKNNVAAHRRHKRVLDRASGNWNGRGKLYRTALETVHKGLK
                     YAYRDRRAKKREFRQLWIARISAAARMCDTNYSQLIAGLKTAGVNLDRKMLADIAARD
                     MATFSSLAKM"
BASE COUNT          432 a          578 c          644 g          264 t
ORIGIN      
        1 gggtgcgcgc cgaggcgagc ctgcggcagg ccctcgaggt gcgccggctg ccgttcgacg
       61 tggacgaggg gggcggggcg ttctacggcc ccaagatcga catcaaaatt aaagacgcgc
      121 tcaaccggtc gtggcagtgc tcgaccatcc agttcgattt caacttgccg gaacggttcg
      181 acatgcacta tattgacgcc gatggccggc cgaagcggcc ctttatgatc catcgcgccc
      241 tgctcggatc gatcgagcgg ttcttcggcg tgctcatcga gcactatgcc gggaagttcc
      301 cgctctggtt ggcgcccgtg caggtgaaag tgcttccgat cacggacagc cagaacgact
      361 acggccgggc gctcgcggac gagctcaagc ggcacggcat tcgcgcgcag gtggacgacc
      421 gatcggacaa gatcggggcc aagatccgcg acgccgaact gatgaaggtg ccctacatgt
      481 tcgtggtcgg cggccgcgag gccgagaatc ggaacgtggc agtgcgcaaa cacggcgtcg
      541 gggatctcgg cgtccggtcc ctggaggagg cccgcgatat gctgctcgcg gaaatccgga
      601 cgaggggact ggcccagagc ggcgccggcg ccaggtaagg aggttctatc agcagcaaag
      661 acttgcgcat caatcgccgc atccgggtgt cgcccgtgcg gctgattggt cccgaaggcg
      721 agcagatcgg cattctgccg accaaagaag cgctcgaacg ggcggaaacc ctcgggctgg
      781 acctcgtgga agtgtcgccc aacagccgcc ccccggtctg ccggatcatg gatttcggca
      841 agtacaagta cgagttgtcg aagaaagacc ggcaggcgaa aaagcggcag cactcgttcc
      901 agctcaagga gatgcgtttc cggcccaaga tcgacgaaca cgacttcgag ttcaagacca
      961 aacacgtgcg caacttcctc gagtcgggga gcaaggtccg ggtgtatgtc atgttccgcg
     1021 gtcgggaaat ggcctaccag gagtacgggc gcaaggtgct tgaccgggtc gcggaagaac
     1081 tgaaagacat cgccaccgtc gagagcgccc ccaagatgga aggccggaac atgaacatgc
     1141 tggtggcgcc gcggcccgag gtgatgaagg tgctcaagca gaaggcggaa cagagagagc
     1201 gggccgagct caaagagcgg gagcgcgcgg ccgaggcggg cgaggccatg cccgacgaac
     1261 agcccgggga gctccaggaa tccccggccg acggcggcgg cgaccggacc ggtgaaaata
     1321 acgagtaacg agagaaggat agcagcacca tgcccaagat gagaacccag cgcgcggccg
     1381 cgaagcggtt taagaagacc gggaccggca agtacaagcg caaacgggcg ttcaagagcc
     1441 acatcctgac gaagaaaacg cccaagcgga tccgcaaact gcgccggccg gcgatcctct
     1501 cacacgccga ccaggggcgg gtcaagcgga tgctgcctaa ctgagatttg acgacagtcg
     1561 taccgaagga gtaccacgca gatggcacgc gcgaaaaaca acgtggccgc ccaccggcgg
     1621 cataaaaggg tgctggaccg ggcctcgggc aactggaacg gccgcggcaa actctaccgc
     1681 accgccctcg aaacggtcca caaaggtctc aagtacgcct accgcgaccg ccgggcaaaa
     1741 aaacgggaat tccgccaact gtggatcgcc cgcatctcgg ccgcggcccg catgtgcgac
     1801 accaactact cgcagttgat cgcggggttg aagacggcgg gggtgaacct cgaccggaaa
     1861 atgctggcgg atatcgccgc ccgcgacatg gcgaccttct cgtcgctggc caagatgg
//
LOCUS       DAOOKS010000419         1918 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_75889_length_1918_cov_2.952228, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000419 DAOOKS010000000
VERSION     DAOOKS010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75889_length_1918_cov_2.952228"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..952)
                     /gene="pta"
                     /locus_tag="PK112_07000"
     CDS             complement(<1..952)
                     /gene="pta"
                     /locus_tag="PK112_07000"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HPC11832.1"
                     /translation="MDAVSLIKERARKKHGTVVLPEGAEPRSIEAAKKLTAEKIARVI
                     LLGDPDKIAAEAKGRGLQLGAVEVITPRRHPEFEAYAHELYEMRKAKGLTPDQAREQM
                     ATELYFGAMLVRHDRADASVAGAVNTTGNVLRAAIHVLGLKPGITTVSSCFLMTIPEY
                     RGEKNKVFVFADCAVVPNPTPEQLASIAVSSADTLRFLLGEEPRVALLSFSTKGSAAH
                     EDVDKVLAALALLKKEHPDLKADGEFQLDAAIVPEVGAKKAPGSAIAGSANVLVFPDL
                     DAGNIGYKLTQRLAKATATGPIIQGLAKPANDLSRGCSADD"
     gene            complement(972..>1918)
                     /gene="prfB"
                     /locus_tag="PK112_07005"
     CDS             complement(972..>1918)
                     /gene="prfB"
                     /locus_tag="PK112_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015253189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HPC11833.1"
                     /translation="ESYERLAADLTAVEELLELADAVESPGELVELEATLDGVEKELA
                     RIESAALLSGPDDHRNAILSIHPGAGGTESQDWADMLFRMYNRWAERNGFTVELMDYQ
                     PGDEAGLKSATLEIKGDYAFGYLKAESGVHRLVRISPFDAAARRHTSFVSVHVFPEVE
                     GNIEIEIREEEVRIDTYRSSGAGGQHVNKTSSAIRLTHLPTGIVVTCQSERSQHKNKE
                     AAFTVLKSRLYQLKREEEAKKMAKFEESKKKIEWGSQIRSYVFQPYQMVKDHRTSVET
                     GNVQAVMDGDLEEFIQAFLTDPELNDSLGALAPTGNTQ"
BASE COUNT          288 a          605 c          640 g          385 t
ORIGIN      
        1 tgtcatcggc cgaacagccg cgcgagagat cgttggcggg tttggcgagt ccctggatga
       61 tcgggccggt ggcggtggcc ttggccaggc gctgggtcag cttgtagccg atgttgcccg
      121 cgtcgagatc ggggaagacc aggacgttgg cgctgccggc gatcgccgaa ccgggggcct
      181 tcttcgcccc gacttccggc acgatcgcgg catccagctg aaattctccg tccgccttca
      241 gatccgggtg ctctttcttc aacagggcca gcgcggccag taccttgtcg acatcctcgt
      301 gcgcggccga acctttggtc gaaaaggaca gcagggcgac gcgcggctcc tccccgagga
      361 ggaagcgcag agtgtcggcc gaggacacgg caatcgaggc gagctgctcg ggcgtcgggt
      421 tgggcacgac cgcgcagtcg gcgaacacga acaccttgtt cttctctccc cggtattccg
      481 ggatcgtcat gagaaagcag ctcgagacgg tggtgatgcc gggtttcagc ccgaggacgt
      541 ggatggcggc gcgcaggacg ttgccggtgg tgttgacggc gccggcgacc gaggcgtcgg
      601 cccggtcgtg gcgcacgagc atggcgccga agtacagctc ggtggccatc tgctcgcgcg
      661 cctggtcggg ggtgagcccc ttggccttgc gcatctcgta cagctcgtgg gcatatgcct
      721 cgaactcggg gtgccggcgc ggcgtgatca cctcgacggc gccgagctgc agcccccggc
      781 cttttgcctc ggccgcgatt ttgtccgggt cgcccaggag aatgacccgc gcgatcttct
      841 cggcggtgag cttcttggcg gcctcaatac tccggggctc ggcgccttcc ggcagcacca
      901 ctgtcccgtg tttcttccgg gcacgttcct ttatgagcga tacggcatcc atgatgcact
      961 ccacagttcg actattgtgt attcccggtc ggcgcgagcg ccccgagact gtcattgagt
     1021 tccggatcag tcagaaaagc ctggatgaac tcctccaggt cgccgtccat caccgcctgc
     1081 acgttgcccg tttccaccga tgtccggtgg tccttgacca tctggtaggg ctggaagacg
     1141 tacgagcgga tctgtgaccc ccactcgatc ttctttttgg actcctcgaa cttggccatc
     1201 ttcttggcct cctcctcccg tttgagctgg tagaggcggg acttgaggac ggtgaaggcg
     1261 gcctctttat tcttgtgctg gctgcgctcg gactggcaag tcaccacgat cccggtcggg
     1321 aggtgggtca gccgaatggc cgaggatgtc ttgttgacgt gctgcccgcc ggcccccgag
     1381 gaacggtagg tgtcgatccg cacctcctcc tcccggatct cgatttcgat gttgccctcg
     1441 acttccggga agacgtgcac ggagacgaag gacgtatggc ggcgggcggc tgcatcgaac
     1501 ggggagatgc gcacgaggcg gtggacgccc gattcggctt tgaggtagcc gaaggcgtag
     1561 tcccccttga tttcgagcgt cgccgatttc agtccggcct cgtcgcccgg ctggtaatcc
     1621 atgagttcga cggtgaagcc gttgcgctcg gcccagcggt tgtacatgcg aaagagcatg
     1681 tccgcccaat cctgcgattc ggtgcccccg gcccccgggt ggatggagag aatggcgttg
     1741 cggtggtcgt ccggtccgga cagcagggcg gccgattcaa tgcgggcgag ctccttctca
     1801 acgccgtcca gggtcgcttc cagttcgacc agctcgcccg ggctctccac ggcgtcggcc
     1861 agctccagca gttcctccac ggccgtgaga tcggcggcga ggcgctcgta actctcaa
//
LOCUS       DAOOKS010000420         1911 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_76294_length_1911_cov_3.788254, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000420 DAOOKS010000000
VERSION     DAOOKS010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76294_length_1911_cov_3.788254"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /gene="rsmH"
                     /locus_tag="PK112_07010"
     CDS             complement(<1..851)
                     /gene="rsmH"
                     /locus_tag="PK112_07010"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HPC11834.1"
                     /translation="MTGPEPIHVPVLRDAAVRLWAARRDGAYLDLTVGAGGHLKALSD
                     YLDGTARLYGIDRDPAAAAAAAAQLAGAPQTVTIARGAYGEFETVTAAFGDRTYDGML
                     MDLGLSSLQLDDPRRGFTFRHDGPLDMRFDPESGGPTAADLVNSLGEKELVDLLHGYG
                     EERRAKRVAQAIVRERRMRMVQTTSQLAGLVERIAPARDLHKTLARVFQAFRIAVNGE
                     LEQLAATMPRLLPRLRAGGRLVVISYHSLEDRLVKRFFQAEAKGCVCPPEAPLCACGR
                     APRLRILT"
     gene            complement(838..1062)
                     /locus_tag="PK112_07015"
     CDS             complement(838..1062)
                     /locus_tag="PK112_07015"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HPC11835.1"
                     /translation="MAAWVTPDRSGRILVPTHLIAEADLKKELLLIGVNKWIEVWNPE
                     RYEDYLRGYEATYEDVAERLFPRPSGDDRT"
     gene            complement(1141..1314)
                     /locus_tag="PK112_07020"
     CDS             complement(1141..1314)
                     /locus_tag="PK112_07020"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MraZ N-terminal domain-containing protein"
                     /protein_id="HPC11836.1"
                     /translation="MNGFVGRYNTTLDEKGRFALPAKLRSILGAGGKPQLDGALILTK
                     GLEGCLSLYTEPE"
     gene            complement(1596..>1911)
                     /locus_tag="PK112_07025"
     CDS             complement(1596..>1911)
                     /locus_tag="PK112_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11837.1"
                     /translation="ASAEERGLSTGRVYTMLASGRPILAWAPEDSELARLLAHTGSGV
                     CFFGSDDSPAVKYLADLVQAHGEGKTPVTLRPAYAEPYSSRRMTEKFAALLDRQKPQS
                     YR"
BASE COUNT          365 a          667 c          534 g          345 t
ORIGIN      
        1 gtgagaatcc tcagccgcgg agcgcgcccg cacgcgcaca gcggcgcctc cggcggacac
       61 acgcaccctt tcgcctcggc ctggaagaac cttttgacca gacggtcctc gagcgagtgg
      121 taggagatca cgaccagccg cccgcccgcc cgcagtcgcg gcagcaggcg cggcatggtc
      181 gcggccaact gctccagttc gccgttgacg gcaatgcgga atgcctgaaa cacccgcgcg
      241 agcgttttgt ggagatcccg ggccggggcg atgcgctcga cgaggccggc cagctgcgat
      301 gttgtctgca ccatccgcat ccgtctctcc ctgactatgg cctgcgccac ccgcttggcc
      361 cggcgctcct cgccgtaccc gtgcagcaga tcgaccagct ccttttctcc cagcgaattc
      421 accagatccg cagccgtagg accgcccgac tccggatcaa aacgcatatc cagcggaccg
      481 tcatggcgaa acgtgaaacc gcgccgggga tcgtccaact gcaggctcga aagaccgaga
      541 tccatcagca tgccgtcgta agtccggtcg ccgaacgcgg ccgtcaccgt ttcgaattct
      601 ccgtaagccc ctctggcgat cgtcaccgtc tgcggcgctc ccgcaagctg ggcggccgcg
      661 gccgccgccg ccgccgggtc ccggtcgatg ccgtacagcc gggccgttcc gtccagatag
      721 tcactcagtg ctttcaggtg gcccccggcg ccaaccgtga gatccaggta cgcgccgtcc
      781 cgccgcgccg cccagagccg cactgccgcg tcccggagca ccgggacatg gatgggctca
      841 ggtccggtca tcgccgctcg ggcgagggaa cagccgctcc gctacatcct cgtaggtggc
      901 ctcgtacccc cggaggtaat cctcgtagcg ttccggattc cacacctcaa tccatttgtt
      961 gacaccgatc agcagcagct ccttcttgag atcggcctcg gcgattaaat gcgtcgggac
     1021 gagaatgcgg ccgctgcgat cgggagtcac ccacgccgcc atcgagaaaa agcggcggcc
     1081 gaagtaacga aaatctcttt gggtaaaact cagtgaggaa agacgcttct cgatatcgtt
     1141 ctattcgggt tcggtgtaca gactcaaaca cccctcgagc cctttcgtca gaatcagcgc
     1201 gccgtccaac tgcggcttgc cgcctgcacc caggatgctc cggagtttgg ccggcagcgc
     1261 gaagcgaccc ttctcatcga gagtcgtgtt ataccggccg acgaacccgt tcacaaggcg
     1321 tttcctccac ttttctccac ttcgacccaa gaacgcccac aaatatcaca ttttattgcc
     1381 tcaagtcaag actttttgtg ccccccgccg gagaaaaaac atcgcggtaa gttccatcac
     1441 ggcaattgat aacgccgaat gaaaattgat gatgaaggaa ccataagacc tgacggttcc
     1501 tgccacccta ttcacttgtg tggataacta tgtggataac tctgcgcgcc cccccattta
     1561 gcccagcatc gaacctgacc ggtcgatccg atcccttacc ggtagctctg tggtttctga
     1621 cggtccagca gtgctgcgaa cttctccgtc attcgccgcg aggagtaagg ttcggcatag
     1681 gccggtcgaa gagtcacagg tgtcttcccc tccccgtgcg cctgcaccag atcggccagg
     1741 tacttcaccg ctggcgaatc atcgctgccg aaaaagcaga ccccgctgcc ggtgtgcgcc
     1801 agcaggcgcg ccagttccga gtcttccggc gcccaagcga gaatcggccg ccccgaagcc
     1861 agcatcgtgt agacgcggcc ggtcgacagc ccccgctcct ccgccgaggc c
//
LOCUS       DAOOKS010000421         1907 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_76536_length_1907_cov_2.665767, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000421 DAOOKS010000000
VERSION     DAOOKS010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76536_length_1907_cov_2.665767"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="PK112_07030"
     CDS             complement(<1..383)
                     /locus_tag="PK112_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11838.1"
                     /translation="MSSFLCVLFLLVNPPPAMGAASVSHFAVPVTRDVRLLPYRVDLI
                     FSGGVSPGAPLVCHPWRVFSAAESASDSVVILATSNQPESSDLPSMVELLRYPERLPL
                     EEHPIWLTVTDAGVWERNGEPPKGL"
     gene            complement(590..940)
                     /locus_tag="PK112_07035"
     CDS             complement(590..940)
                     /locus_tag="PK112_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPC11839.1"
                     /translation="MAAKRTKYFFQASNQQVDLVARAFRALGDPTRLKMLKALESGEQ
                     SVQDLVAQFTCSQPNISRHLSILTAAGLVAKNKQGPYVLYRVADPRIFELCDLVCGHV
                     YSLLARYSGKASSA"
     gene            complement(1069..>1907)
                     /locus_tag="PK112_07040"
     CDS             complement(1069..>1907)
                     /locus_tag="PK112_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015906503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPC11840.1"
                     /translation="RTGVLVPLAELVTVREAIVDKTIYRKNLMPVTYVIGDVAGAIES
                     PVYAIGEMNRKIAALNARDFGGTLPALKVYNASMPFTDLEPSLKWDGEWHITLEVFRD
                     LGIAFAAVLVLIYVLLVGWFRSMVTPIILMVVIPLSLIGILPAHGAMDAFFTATSMIG
                     FMAGAGIVIRNSIILIDFAELRLREGSTLAEAVIDAGAVRFRPILLTAGAVVVGAGII
                     LFDPIFQGLAIALIAGEVASLVISPVAVPLLYYLVNRTKAAAGGGSESQTAPTEGAPE
                     TL"
BASE COUNT          350 a          644 c          625 g          288 t
ORIGIN      
        1 cagagcccct tcggcggctc tccattgcgc tcccagaccc cggcatcggt gacggtcagc
       61 cagatgggat gttcctccag ggggagccgt tccgggtagc gcagcagttc caccatcgac
      121 ggcaagtcgc tcgattccgg ctggttggat gtcgcgagga tcaccacgga gtcgctcgcc
      181 gactccgcgg cgctgaagac tctccagggg tggcacacca gcggggcgcc cgggctaacc
      241 ccgccggaga aaatcaggtc gacccgatag ggcagcaggc gcacatcgcg cgtcaccgga
      301 acggcgaaat gggagaccga tgccgcgccc atcgcggggg gaggattcac gagcaggaaa
      361 agcacgcaca ggaaggaact cacgccagaa acctccgccg gacaggagac acttttctgc
      421 tctcacccgg atgccgctgc cggagaaggg aaggatccgc agcacccgcc gggaagatac
      481 aatactgcaa tatacgcaag cgggtcccga atggaaagac ccattgcgcc cgtccccgga
      541 ggatcttggc cgagcctcgc cggaggccgc cactgcggtg gcggaccagc tacgccgagg
      601 acgccttgcc gctgtaccgc gccagcaggc tgtagacgtg cccgcacacc aggtcgcaca
      661 gttcgaaaat gcgcggatcc gccacccggt acaggacgta cggaccctgc ttgtttttgg
      721 ccaccagccc ggccgcggtg aggattgaga ggtgccgcga aatgttgggc tgggagcaag
      781 taaactgggc caccagatcc tgcaccgact gctcgcccga ttcgagcgcc ttgagcattt
      841 tcagccgcgt cgggtcgccc agcgcccgga aggcgcgggc gaccagatcc acctgctggt
      901 tgctcgcctg gaaaaagtac ttggttcgct tggctgccat gttccgggta ctcccgtctc
      961 gtcttgattc cgtcactgca caatgccggg cgcgatcctg cctccgcctg ccgcccgccc
     1021 gggccgggcg ggtgggtttg gcttccggct ctgcgccggc cggtcgcgtc acagcgtctc
     1081 cggcgccccc tcggtcggcg ccgtctggga ttccgatcct ccgcccgccg ccgccttcgt
     1141 gcggttgacc aggtagtaca ggagcggcac ggcgaccggg ctgatgacga gcgaggcgac
     1201 ctcgccggcg atgagcgcga tggccaggcc ctggaagatc gggtcgaaga ggatgatgcc
     1261 ggcgcccacg accaccgcgc ccgccgtgag cagaatgggg cggaatcgca ccgcgccggc
     1321 gtcgatcacc gcctcggcca gggtgctgcc ctcacgcagg cgaagctcgg cgaagtcgat
     1381 caggataatt gaattgcgga tcacaatccc ggcccccgcc atgaaaccga tcatcgaggt
     1441 cgccgtgaaa aatgcatcca tcgccccgtg ggccggcaga atgccgatga gcgagagcgg
     1501 gatcaccacc atcagaatga tcggcgtgac catcgaccgg aaccatccca ccagcaggac
     1561 atagatgagc acgaggaccg cggcgaacgc gatgcccagg tcgcggaaca cctcgagcgt
     1621 gatgtgccac tcgccgtccc acttcagcga gggctcgagg tcggtgaacg gcatcgacgc
     1681 gttgtacacc ttcagcgccg ggagcgtccc cccgaaatcg cgggcgttga gggcggcgat
     1741 cttccggttc atctccccga tcgcgtagac gggggactcg atcgcgccgg cgacgtcgcc
     1801 gatgacgtag gtcaccggca tcaggttctt gcggtagatc gtcttgtcga cgatcgcctc
     1861 ccgcaccgtc acgagctcgg cgagcggcac cagcaccccg gtccggg
//
LOCUS       DAOOKS010000422         1904 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_76698_length_1904_cov_2.999459, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000422 DAOOKS010000000
VERSION     DAOOKS010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76698_length_1904_cov_2.999459"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="PK112_07045"
     CDS             <1..736
                     /locus_tag="PK112_07045"
                     /inference="COORDINATES: protein motif:HMM:NF018675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HPC11841.1"
                     /translation="PYQFFHGLEGVSRLSGVHWEDRARALMAPFDGSVIRWTAEARHE
                     VIRFSGGATDRRHVLRVLDLFGFSLETAAMTTSEALFWLTVMPTFNNWSGNPARVARI
                     LQAVFGLPFSIAENVPLPQEIPQDIQTRLGQQFSRLGNELVLGREYTDRDNGCEVVVH
                     EVPADRAADWIEGGACRQRLANILEFSMPGHMDCRIRIEVREEPAPLGEGPAGAYLGA
                     SLRLHREPGAAAVRPVNEVDSTGSRL"
     gene            1302..>1904
                     /locus_tag="PK112_07050"
     CDS             1302..>1904
                     /locus_tag="PK112_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11842.1"
                     /translation="MYATDGAYEKHKEYCNRLESLMAVISRAHLVAATAPPAGGRNEL
                     PAGIEALCDRVGAQLAATFDLLALGPAAPHPHEVVVFFKRLFRTVATALAYRPAVKDY
                     LNDRLFSRTLGSSMGAFVSQIDSFLLTEYNHRGLSEHFHAIDAVLRLLQETFSFLAQS
                     DRIAPEPSITETVEYQGRTYAMAATSGLRCEERDGLCYLLV"
BASE COUNT          289 a          710 c          598 g          307 t
ORIGIN      
        1 cccttaccag tttttccacg gcctggaggg tgtcagccgc ctgagcggcg tgcactggga
       61 agaccgcgcc cgcgccctca tggcgccctt cgacggttcc gtcatccgct ggaccgccga
      121 ggctcgccac gaggtgatcc ggttttccgg cggcgccacc gaccgccgcc acgttctccg
      181 cgtgctcgac ctcttcggtt tcagcctgga aacggccgcc atgaccacct ccgaggccct
      241 cttctggctg accgtcatgc ccactttcaa caactggtcg ggaaaccccg cgcgggtcgc
      301 gcgcatcctc caggcggtgt tcggcctccc gttctccatc gctgagaacg tccccctccc
      361 gcaggagatc ccccaggaca ttcagacccg cctgggacag cagttcagcc ggctgggaaa
      421 cgagctggtt ctcggccggg agtatacgga ccgcgacaac gggtgcgagg tggtcgtgca
      481 cgaggtcccc gccgaccgcg ccgcggactg gatcgaggga ggcgcctgcc ggcagcgcct
      541 ggcgaacatc ctcgagttta gcatgcccgg acacatggac tgccgcatca ggatcgaggt
      601 gcgggaagaa cccgccccgc tgggggaagg ccccgccggc gcctacctcg gggcctcgct
      661 gcggctccac cgcgagccgg gggcggccgc cgtacgtccg gtcaatgaag ttgactccac
      721 ggggagcagg ttgtagattc ggatgaagga atcggggaag aatggatgag taatttccgc
      781 ttgtactcgg tcaactggca ggacggcatg ctcgtcgccc gcaaacacct cctcgacgag
      841 gagcgctacg tcgaggatct ggcccgctgg tacgcctatc cggtggggga gcgctacggg
      901 ctggcgcgca agtcgtctga cgggcggccg gcgctcggcc tgaccgtgct cgtgcacggc
      961 gcccgggtgc gcgtcgaact cagccgctgc caggccatca cctccgacgg gacggtcatc
     1021 gagatcaacg aatccggccc cgaggtgctc tttgctgagg cgcccttgca gggggaccgg
     1081 atcgacgtgt tcctcgcggt caatcgcgag gccaagcggc cggtcggggc gcccgatgcc
     1141 cgtgagctgg tcccgcgcgc gcccttcgcc gcccgcgact acgcctccac ctcggcgaac
     1201 cgcccgcgct gccggaatct gatttcctcc agatcgcccg gcacaagatc gccggcggcg
     1261 acgtcgtcca ggcgcccgac tactatcccg cgtgcctgtc gatgtacgcg accgacggcg
     1321 cctacgagaa acacaaggag tactgcaacc gcctggagag cctcatggcg gtcatctcgc
     1381 gcgcccacct ggtggccgcg accgcccccc cggccggcgg ccgcaacgag ctcccggcgg
     1441 ggatcgaggc gctctgcgat cgcgtcggcg cgcaactggc ggccaccttc gacctcctcg
     1501 ccctcggacc cgccgcgccc caccctcacg aggtcgtggt cttcttcaag cgcctcttcc
     1561 gcactgtcgc caccgcgctc gcctaccgcc cggcggtcaa ggactacctc aacgaccggc
     1621 tcttctcccg caccctcggc tcgagcatgg gcgccttcgt ctcccagatc gacagcttcc
     1681 tcctcaccga atacaaccac cgcggtctct cggagcattt tcacgccatc gacgccgtcc
     1741 tccgcctgct ccaggagacc ttctccttcc tcgcccagag cgaccggatc gcccccgaac
     1801 cgtcgatcac cgagaccgtc gagtaccagg gacgcacgta tgcgatggcg gcgacttcgg
     1861 gcctgcgctg cgaggagcgc gacggtctgt gctacctgct ggtc
//
LOCUS       DAOOKS010000423         1901 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_76893_length_1901_cov_2.155471, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000423 DAOOKS010000000
VERSION     DAOOKS010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76893_length_1901_cov_2.155471"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            542..1081
                     /locus_tag="PK112_07055"
     CDS             542..1081
                     /locus_tag="PK112_07055"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial transcriptional activator
                     domain-containing protein"
                     /protein_id="HPC11843.1"
                     /translation="MYDLYFGLGLYHYWKSAKAGVLRWLGIVSDDKERGLRELETAAE
                     GSLISREAARSSLVWVRLDRKEYREAVALGDSLMRRYPHSRSFLWPVAEAQYRLGRYA
                     DAAAAYGRLREMLREEPGNYYNLIECDYRIARCFEMMGLPGEWAGTAADVSGYMGEVS
                     EETQERQRERLEYLGVAGE"
     gene            complement(1273..>1901)
                     /locus_tag="PK112_07060"
     CDS             complement(1273..>1901)
                     /locus_tag="PK112_07060"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HPC11844.1"
                     /translation="LLEKGIEDILPVDAGLDLLVVKMQKIQAVLQAKQKQRETADHKS
                     GATGRLADMNLIDLLQALGPSRRTARITVTPGPSAARADDSRPGLEPDPRGGFSDNSR
                     AGRSGDAASAGSTPGRPDRRPDQKDRRLNDHLILYLDRGAIVFAQYRDKIGAEAVYET
                     IGWADGYWSVEPVSREALPAPNNDLSNESILMEGCRLLDERLHAGKLF"
BASE COUNT          320 a          559 c          708 g          314 t
ORIGIN      
        1 aatcgccgac cctattggcc gatactaggc atgtgacggt ccggctcaca tactcccggc
       61 gtgcgacggc cgcggtcgtc gccgtagtcc tggcggcgct cctccccgcg cacacgagcg
      121 cacgggcagc cgcgtacccg gattcccagc aggtgcggct gctggccgcg cttcaggaag
      181 acctctacaa cgaccggtac gacgcagccg agaaggtcgc gcgacttatg gtgtcctcgt
      241 acccggatga cccgctggga cacctgttca cgggcatcac gctggtcgcg gcggcctacg
      301 acgccgagga ggcgctgcgc gaacgcgagt tccgcacaag cctcgccgcg gcggagcgct
      361 tggcgggatt acagatcgcg tcgggagatc ggcacacgcg ggcctgggga ttcctcgtgc
      421 tcggacacgc cgcctcctac cgcgcggtct gggaagccaa gttcggatca aagctcaccg
      481 ccgcgcgcgc cgggagaacc gcggccggac tctacgaacg gggactgcgg ccgacagcac
      541 ggtgtacgac ctgtactttg ggttggggct gtaccattac tggaagtcgg cgaaggcggg
      601 agtactgcga tggctgggaa ttgtatccga tgacaaggaa cggggcctgc gggagctgga
      661 gacggcggcg gaggggtcgc tcatttcgag ggaggccgcg agatcatcgc tggtctgggt
      721 gcggctggat cgtaaggagt accgggaagc ggttgcgctc ggcgacagcc tgatgcgccg
      781 ctacccgcac agccggagct tcctgtggcc ggtggccgag gcgcagtatc gcctgggacg
      841 ctatgccgat gcggcggcgg cgtacggccg gttgcgggaa atgctgcgcg aagagcccgg
      901 caactattac aacctgatcg agtgcgacta tcggatcgcc cggtgctttg agatgatggg
      961 cctgccgggc gaatgggcgg gcacggccgc cgatgtgtcg ggatacatgg gagaggtgtc
     1021 ggaagagacg caggagcggc agcgggagcg gctggagtat ctgggggtgg ccggggagta
     1081 agccgccggt gaaggcgatc gcgacacggg ccggataacc tccgcgaaat aggtcttgta
     1141 agtcaggacc tttatgatcc tgacggggct gctcacatcg atggtcagaa tgtcaggacc
     1201 acaagggtcc tgacccacaa gagatccggg ggacggctgt tacctgtgtg cccggcgcca
     1261 tcggaggaaa ggtcaaaaca acttgccggc gtggaggcgt tcgtcgagga ggcggcagcc
     1321 ttccatgaga atcgactcat tggagaggtc gttgttcgga gcgggaaggg cctcgcggga
     1381 caccggttcc accgaccagt acccatccgc ccagccgatt gtttcataga ccgcctcggc
     1441 gccgattttg tcccggtact gggcgaagac gatcgctccg cggtcgaggt agaggatgag
     1501 atggtcgttc aggcggcggt ctttctggtc ggggcggcga tcgggccggc cgggagtgct
     1561 tccggcgctg gcggcgtcgc cggaacggcc ggcgcgcgaa ttgtcggaga aaccgccgcg
     1621 cgggtcgggc tcaaggcccg ggcgagaatc gtcggctcgg gcggcggagg ggccgggggt
     1681 gacggtgatg cgggccgtcc tgcggctcgg gccgagggcc tgcaggaggt cgatgagatt
     1741 catgtcggcg agacggccgg tggcgccgga cttgtggtcg gcggtttcgc gctgcttctg
     1801 cttcgcctgc agcaccgcct ggatcttctg catcttcacc accagcaggt cgaggccggc
     1861 atcgacggga agaatgtcct cgatcccctt ctccaggagc c
//
LOCUS       DAOOKS010000424         1897 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_77137_length_1897_cov_2.307818, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000424 DAOOKS010000000
VERSION     DAOOKS010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77137_length_1897_cov_2.307818"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1890)
                     /locus_tag="PK112_07065"
     CDS             complement(<1..1890)
                     /locus_tag="PK112_07065"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HPC11845.1"
                     /translation="MSFGDAARPTLLKLSLDKQSYSPGETAHLHVDSPFDGRGVLVIQ
                     GEEIRGMIPFEIKENRAEIPIPIVKETVPNLWLEVTALHEVKTDRAQVYPFSSFAMTP
                     LMVTNLDRKLEVTFPELPEEVRPLTDARFDVLVKDGNGDPVEAELTLAAVDEGIHAIT
                     DYQNPDPYGFFSRPRKPDYRRAHYYDKVAYDFDNPGFGGDGQLRRRAIHIGENWIKPV
                     ALWSGVVKTSAEGKASITLSLPEFAGQLRLVAVGSSKTASGTGARDLFVRRPFILRTS
                     VPRFLLPGDSAECTAVVFNTTDQPASATVSWAVSGPLRAESGEQKIAIQGKGEARVTA
                     RFTAEQAVGQGMIIWRAVVTDADGNPLEDLKEEAAMPIRPSSGYQSQHDFTTLEPGKS
                     QTFANELFLENDYTEVALSVTADPTSRLERSVRFLSHYPYGCIEQTVSGLMPLYLLRT
                     RRDMVRRLMPNDEDLNTRIEAGIQRIFTIQTPNGGLSFWPGGYEPYPYGSLYALHFLS
                     ILKNDRQFTIPDAPYKGLQRYARSVASDWSDNSESHLYQRAYALYVLALGGDAEAVKQ
                     IRRFDNIIMPQPGRYLLAAALAYAAPFGGAALVALALAQAAMLAPVAPFADAVGVSAA
                     SRGHVG"
BASE COUNT          343 a          586 c          625 g          343 t
ORIGIN      
        1 accgacatgg ccgcgggacg ccgccgaaac tccgacggcg tcggcgaagg gcgcgaccgg
       61 ggccagcatc gcggcttgcg cgagcgcgag cgcgacgagc gccgccccgc cgaaaggagc
      121 ggcataagcg agcgcggcgg ccagcagata gcggcctggc tgcggcatga tgatgttgtc
      181 gaaacgccgg atctgcttta cggcctcggc gtcgccaccc aacgccagga catacagcgc
      241 gtaggcccgc tggtacaggt gcgactccga attgtccgac cagtccgatg cgacggagcg
      301 tgcgtaccgt tgcaggccct tgtagggcgc gtcgggaatg gtgaactggc ggtcgttttt
      361 cagaatactg aggaaatgaa gggcgtacag cgaaccgtag gggtacggct cgtaaccgcc
      421 gggccagaag ctcagcccgc cgttgggggt ctgtatcgtg aagatccgct ggatccccgc
      481 ctcgatgcgc gtgttcaggt cttcatcgtt gggcatcaga cggcggacca tgtcgcgccg
      541 cgtccgcagc aggtacaacg gcatcagtcc ggagaccgtc tgctcgatgc atccgtaggg
      601 gtagtgtgag agaaaacgaa ccgagcgttc gaggcgtgac gtcggatctg ccgtgaccga
      661 gagcgcgacc tccgtgtaat cgttctcgag gaacagctcg ttcgcgaacg tctggctctt
      721 gcccggctcg agggtcgtga aatcatgctg cgactgatac cccgacgagg gccggatcgg
      781 catggcggct tcttccttga gatcttcgag tggattgcca tccgcatccg tcaccacggc
      841 gcgccaaatg atcataccct ggcctacggc ctgttccgcg gtaaaccggg ccgtgacacg
      901 ggcctcgccc ttaccctgaa ttgcgatttt ctgctcaccc gactcggcgc ggagcggtcc
      961 cgagaccgcc caggacacgg tggcggatgc gggttgatcg gtggtgttga aaaccaccgc
     1021 cgtgcattcc gccgaatcgc ccggcaagag gaaccggggc acactggtcc gcagaatgaa
     1081 gggacgccgc acaaaaagat cccgggcgcc ggttccgctg gccgttttcg agctacccac
     1141 cgccacgagc cggagctgtc ccgcgaattc cggcagcgac agggtgatgg acgccttgcc
     1201 ttcggcgctg gtcttcacaa cccccgacca tagcgccacg ggtttgatcc agttctcgcc
     1261 gatatggatt gcgcgccggc gcaactggcc gtcgccgcca aaccccggat tgtcgaagtc
     1321 atacgccacc ttgtcgtagt agtgggcccg ccggtagtcg ggcttccgcg gacgcgagaa
     1381 aaacccgtag ggatcggggt tctgatagtc cgtgatggcg tggatgcctt cgtccacggc
     1441 cgccagggta agctcggcct cgacgggatc gccgttgccg tccttgacga gcacatcgaa
     1501 tcgcgcgtcc gtgagcggcc ggacttcttc gggcagttcc gggaacgtca cctcgagttt
     1561 gcggtcgagg ttagtgacca taagcggtgt catcgcgaaa ctcgagaagg ggtagacttg
     1621 cgcgcggtcc gtcttgacct cgtgcagcgc cgtgacctcc aaccacaggt ttggcacggt
     1681 ctctttcacg atggggatgg gaatctcggc tctgttctcc tttatctcga agggaatcat
     1741 gccgcgaatc tcttcgcctt gaatgacgag cacgccgcgc ccatcgaacg gcgaatccac
     1801 gtgaaggtgg gcggtctcgc cgggcgaata ggactgtttg tcgaggctca gcttcaggag
     1861 ggtcggccgc gcggcatcgc cgaaagacac gcgtccg
//
LOCUS       DAOOKS010000425         1895 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_77244_length_1895_cov_3.671196, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000425 DAOOKS010000000
VERSION     DAOOKS010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77244_length_1895_cov_3.671196"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="PK112_07070"
     CDS             <1..320
                     /locus_tag="PK112_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003967906.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HPC11846.1"
                     /translation="PTLMHGKTSAITHDRSALFAGIDSPFTATRYHSLMVAPAGLPRV
                     FKVTARSADGVIMALQHRRRPLYGVQFHPESILTAAGRRLLANFLKLCLGQGENGEGR
                     GRK"
     gene            408..1424
                     /gene="trpD"
                     /locus_tag="PK112_07075"
     CDS             408..1424
                     /gene="trpD"
                     /locus_tag="PK112_07075"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019005461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HPC11847.1"
                     /translation="MITELLQKLLRGEHLTTDEAKQAMNLIMCGEVTPAQIGGFVIAL
                     RQKGETPEEVAGFVEAMRSLSLRIELDRTRETAVDGCGTGGDGANTFNISTAASLVVA
                     AADIPVAKHGNRSVSSKCGSADLLAGLGADIDPGPEVVKENIERVGFGFLFAPRYHPA
                     MRHAALPRRELGVRTIFNMLGPMTNPAGVKRQVIGVYSRQVMYLMAEVLALLGSRHVL
                     VAHSRDGLDEFSITAPTDYIEVRDGWIAKGTLNSEDVGLPERYPGVLEGGDIARNAAI
                     LEDVLAGRESAYRDAVALNAGAMIYVADRAESIREGIVVAQRAIDSGGAKDLLDRWRA
                     ATHS"
     gene            1428..>1895
                     /locus_tag="PK112_07080"
     CDS             1428..>1895
                     /locus_tag="PK112_07080"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPC11848.1"
                     /translation="MKSAEPQLDPAPPSARSGPPFVSGLRDYLEVIRLFSRNAWLFLI
                     GSFLMGINFQVFNLLLNLYLKELGYAEGDIGLVASGRAIGMTAMAIPVAVVLSRVRLK
                     PILLATVAIFAFFSVFIVTLDRLPLLVGSAVLSGMAFAFYRVAGGPFYMRNSTA"
BASE COUNT          291 a          633 c          609 g          362 t
ORIGIN      
        1 ccccgacgct catgcacggc aagacctccg ccatcaccca cgaccggtcg gcgctttttg
       61 cggggattga ctcgcccttc acggccacgc gctaccattc gctcatggtc gccccggccg
      121 gtctgccgcg ggtgttcaag gtcacggccc ggtcggccga tggtgtcatc atggcgctgc
      181 agcaccgccg ccggccgctc tatggggtgc agtttcaccc cgagtcgatt ctcaccgccg
      241 ccggccgccg gctcctggcg aacttcctga aactctgcct tggccagggc gaaaatggcg
      301 aggggcgcgg ccgaaaatga agtttgccgg ttgaggcggc cgccgttccg agattggctg
      361 cagacgcacc agggcccggc gccggccgct tcgccacaat cgaaacgatg attaccgaac
      421 tgctccagaa gctgctccgc ggcgagcacc tgacgaccga cgaggccaaa caggcgatga
      481 acctcatcat gtgcggcgag gtcacgccgg cccagatcgg cggttttgtc attgccctgc
      541 gccagaaagg ggagacgccg gaagaggttg cgggctttgt cgaggccatg cgcagcctgt
      601 cgctccgcat cgaactggac cgcacccgcg agacggcggt cgacggctgc ggcacgggcg
      661 gcgacggcgc caacaccttc aacatttcca ccgccgcctc gctcgtcgtt gcggccgccg
      721 acatcccggt cgccaaacac ggcaaccgct cggtcagctc caaatgcggc tcggccgatc
      781 tgctggccgg tctcggcgcc gacatcgacc ccggcccgga ggtggtcaag gagaacatcg
      841 agcgggtcgg gttcggcttt ttgtttgccc cgcgctacca cccggccatg agacacgccg
      901 ctctcccgcg ccgggagctg ggggtccgca cgatcttcaa catgctcggt cctatgacca
      961 acccggccgg cgtcaaacgc caggtcatcg gcgtttacag ccgccaggtc atgtacctca
     1021 tggccgaggt gctcgccctg ctcggctccc gacacgttct ggtcgctcat tcccgcgacg
     1081 gtctcgatga attctcgatt accgccccga ccgactacat cgaggtacgg gacggctgga
     1141 ttgcgaaggg gacgctcaat tccgaggatg tcggtctgcc cgagcgctac cccggtgtgc
     1201 ttgagggcgg ggatatcgcc cggaacgcgg cgattctcga agacgtgctc gcgggccggg
     1261 aatcggccta ccgcgatgcg gtggcgctca acgccggggc gatgatttac gttgctgaca
     1321 gggccgagtc gatccgcgag ggcatcgtgg tcgcccagcg ggcgatcgat tcgggggggg
     1381 caaaagatct gcttgaccgc tggcgggcgg cgacgcactc ttaatcaatg aagtccgccg
     1441 aaccgcagct tgatcctgca ccgccttcgg cccgttcagg tccgcccttc gtctccgggc
     1501 tgagagacta cctggaggtg atccgcctgt tttcgcggaa cgcctggctc tttttgatcg
     1561 ggtcgtttct catggggatc aatttccagg tcttcaacct cctgctgaat ctgtacttga
     1621 aggagcttgg gtacgccgag ggggatatcg gcctggtggc ctccgggcgg gcgatcggca
     1681 tgaccgccat ggctattccg gtggcggtgg tgctcagccg ggtccggctc aaaccgattc
     1741 tcctggccac cgtcgccatc ttcgctttct tctcggtgtt tatcgtgacc ctggaccgcc
     1801 tgccgctgtt ggtcggttcg gccgttctct cggggatggc gtttgcgttc taccgggtgg
     1861 cgggggggcc cttctacatg cgcaattcca ccgcc
//
LOCUS       DAOOKS010000426         1894 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_77318_length_1894_cov_2.231648, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000426 DAOOKS010000000
VERSION     DAOOKS010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77318_length_1894_cov_2.231648"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /gene="gcvPA"
                     /locus_tag="PK112_07085"
     CDS             complement(<1..827)
                     /gene="gcvPA"
                     /locus_tag="PK112_07085"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HPC11849.1"
                     /translation="MPYVPNTDDDRRDMLKRIGVDKFEDLLEGIPSELRLKRELNIPA
                     LSEMELLREIEALSRESREGLAGFAGGGVYDHFIPAAVGAITSRPEFMTAYTPYQAEV
                     AQGTLQAIYEYQTHVCRLTGMDVANASMYDGATAAAEAAMLAVKATGRNRMVAAETLN
                     PMYREVIRTYLSGLDVEIVTAPMADGVTDLNRLEDAIDEHTAGVILAQPNFFGRLEEI
                     GPVEQMIHRVGGKLVLAFDPIAQAVLKTPGEWGADIAVGEGQPLGLPVSFGGPLLGM"
     gene            complement(957..1339)
                     /gene="gcvH"
                     /locus_tag="PK112_07090"
                     /pseudo
     CDS             complement(957..1339)
                     /gene="gcvH"
                     /locus_tag="PK112_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446355.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
     gene            complement(1366..>1894)
                     /locus_tag="PK112_07095"
     CDS             complement(1366..>1894)
                     /locus_tag="PK112_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11850.1"
                     /translation="IKPRWAVIECRINAEDPEKDFRPTPGTITGLHIPGGPGVRVDRA
                     VYTGYQIPPHYDSMIAKLIVRGRTRQEAVVRMRRSLEEFIVEGVPTTVAFHREIFEHP
                     DFVAGRYDINFLEQRFRKGQEAPPAPEEAQEEAIAAGETVSRLTLLRGSEAGVLAEEE
                     TVETGAPDSASDNKQ"
BASE COUNT          275 a          671 c          586 g          362 t
ORIGIN      
        1 aacatcccca gaagcggccc gccgaacgac accggcagtc cgaggggctg cccctcgccc
       61 accgcgatgt cggcgcccca ctctcccggc gtcttgagca cggcctgggc gatcgggtcg
      121 aacgccagca ccagcttgcc gcccacgcgg tggatcatct gttcgaccgg gccgatctcc
      181 tcgagccggc cgaagaaatt cggctgggca agaatcacgc cggccgtgtg ctcatcgatc
      241 gcgtcctcca gccgattcag gtcggtcacg ccgtcggcca tcggcgctgt cacgatctcc
      301 acgtcgagac cggagaggta ggtccggatc acctcccgat acatcgggtt gagcgtctcg
      361 gccgccacca tgcggttgcg cccggtcgct ttgaccgcca gcatggcggc ctcggccgcc
      421 gccgtcgccc cgtcgtacat cgaggcattc gccacgtcca tcccggtcag acggcagacg
      481 tgggtctggt attcgtagat cgcctggagc gttccctgcg ccacctccgc ctggtaggga
      541 gtgtaggcgg tcatgaactc cgggcggctg gtgatcgcgc ccacggccgc ggggatgaaa
      601 tggtcataga cgccgccccc ggcaaagccc gccagaccct cgcggctctc gcgggagagc
      661 gcttcgattt cccggagaag ctccatctcc gagagcgccg ggatgttgag ttcgcgcttg
      721 aggcgcagct ccgagggaat cccctccagc aggtcctcga acttgtccac gccgatgcgc
      781 ttgagcatgt cgcggcgatc atcgtcggta ttcggtacgt acggcatcgc tgtttacttc
      841 cgatcctttg tgttgtttcg cccgattccg ggggtcgctg gtgcgctttc ggggcggccc
      901 tcccggcgcg ccggcccgga gtttacccgg gggccgggcc ggtgcggcgc ctgggcctag
      961 accgccgcca gcagtttctg ataacccgcg gcgtcgagca gggcgtccag ctggccgggg
     1021 tcggacacct caaccttgat catccagccc tcgccgtagg ggtcgcggtt gatcaccatc
     1081 ggatcgtcct cgagcgcgcc gttgaccgcc gtcacgcgtc ccgaaacggg cgcgaacatt
     1141 tccgagaccg ccttcacggc ctcgatcgtg ccgaacgcct tcatctgtgt caccacactg
     1201 ccgactctgg gcagttcgac gaagacgata tcgcccagtt cccctgggcg tagtcggtaa
     1261 tcccgatggt cgccgtttgt ccttcgcggc gaatccactc gtgctctttc gtatacttga
     1321 gttccgcggg tatgttcacc ggatcctcca atgagcctca cgtccttact gcttgttatc
     1381 cgatgccgag tcgggcgcgc ccgtctcgac cgtttcttcc tccgccagca ctccggcctc
     1441 cgacccccgc aagagcgtca gccggctcac tgtttccccc gccgcgatcg cctcctcctg
     1501 cgcctcctcc ggcgcgggcg gggcctcttg tcccttgcgg aagcgctgct cgaggaaatt
     1561 gatgtcgtag cggccggcga cgaagtcggg atgttcgaaa atctcccgat ggaacgccac
     1621 cgtggtcggc acgccctcga cgatgaattc ttccagcgag cggcgcatcc ggaccacggc
     1681 ctcctggcgc gtccgccccc tgacgatcag tttcgcaatc atggaatcgt agtggggcgg
     1741 gatctgatag ccggtgtaca cggcccggtc cacccgcacg cccggtcctc cgggaatgtg
     1801 cagcccggtg atcgtccccg gcgtcggccg gaaatccttt tcggggtcct cggcgttgat
     1861 gcggcactcg atgaccgccc agcggggctt gatc
//
LOCUS       DAOOKS010000427         1893 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_77358_length_1893_cov_3.170294, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000427 DAOOKS010000000
VERSION     DAOOKS010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77358_length_1893_cov_3.170294"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..526)
                     /locus_tag="PK112_07100"
     CDS             complement(8..526)
                     /locus_tag="PK112_07100"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin fold domain-containing protein"
                     /protein_id="HPC11851.1"
                     /translation="MKAITPIMLPLVLVLAAGAFVFADDESGKTAAPAEIQWVAYDVG
                     LQRAAAEKKHVLVDFTAKWCGFCKKMDRETFAKPEVVEMINANFIPVKVDGDSQKELN
                     VNGYKITEANLAKKEFGVRGYPTFWFLKSDGSKLGQITGYRPPDVMMEALSYVKDEQY
                     DTTKTETPKSND"
     gene            complement(552..>1893)
                     /locus_tag="PK112_07105"
     CDS             complement(552..>1893)
                     /locus_tag="PK112_07105"
                     /inference="COORDINATES: protein motif:HMM:NF025930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HPC11852.1"
                     /translation="AMGKKDAALMAAQKQEFLEGADRLGVPRSVSEEVFHQIETFARY
                     GFNKAHSTCYAYVAYQTAWLKQYYPKEFMAALMTSEINDSDRIYAFLEECRRANIDVL
                     PPDVNHSQVDFSVAEGKIRFGLQAVKNVGGSPALAIVEERRRDGLYSDLADLVTRVPA
                     KTLNRRVLESLVAAGAADSLPGNRAQKYAAVEAVLEFAHKVQEAAASHDLFADSAGAR
                     RVPPALPPLEEWPRAERLNQEKTMLGFWISGHPLDAYRDELKSFTTFTADGLRQAADG
                     REVTVGGILTAVKKMVDKKGNMMAFATLEDYTGSTELILFSKCYDENKEIIAADRMVL
                     VTGRVSTREGEAPKIIGGEVLPLEKLTERFSCQLVIKIDVDCPDTVLDQTLRTLDASR
                     GSTPVLVAARENGSEVYIRSRKYAVNPDFALLNSLKAILGESGAYLRPLQRKES"
BASE COUNT          294 a          551 c          659 g          389 t
ORIGIN      
        1 gcgggggcta gtcgttactc ttgggagtct ccgtcttggt agtgtcgtac tgctcgtcct
       61 tcacgtacga gagggcttcc atcatgacgt cggggggacg gtatccggtg atctggccaa
      121 gcttgctgcc gtcggacttg agaaaccaga acgtggggta gccgcggacg ccgaattctt
      181 tcttggcgag gttggcttcg gtgatcttgt agccgttgac gttgagttcc ttctgggagt
      241 cgccgtcgac tttcaccggg atgaagttgg cgttgatcat ttcgacgact tcgggtttgg
      301 cgaaagtttc gcggtccatc ttcttgcaga agccgcacca cttggcggtg aagtcgacga
      361 ggacgtgctt tttctcggcg gcggcgcgct ggaggccgac gtcgtaggcg acccactgga
      421 tttcggccgg ggcggcggtt ttgccggact cgtcgtcggc gaacacaaac gcgccggcgg
      481 cgaggacgag cacaagcggg agcatgatgg gcgtgatggc tttcatgagg caccttgttg
      541 cggtatggat gttaactttc tttgcgctgc agggggcgca ggtacgcgcc gctttcgccg
      601 aggatggctt tcagggagtt cagcagggcg aaatcggggt tgacggcgta cttgcgcgac
      661 cggatgtaca cctccgagcc gttttcgcgg gccgccacga gcaccggggt acttccccgg
      721 ctggcgtcca gcgtgcggag cgtctgatcg agtacggtat cggggcaatc cacatcgatc
      781 tttataacca actggcagga gaagcgttcc gtcagcttct ccagcggcag aacctcgccg
      841 ccgatgatct tcggagcctc cccctcgcgc gtcgacaccc gcccggtcac cagcaccatc
      901 cggtcggccg cgatgatctc cttgttctcg tcgtaacatt tcgagaagag aatcagttcg
      961 gtcgaaccgg tgtagtcttc gagggtggcg aacgccatca tgttcccttt cttgtcgacc
     1021 attttcttga cggcggtgag gatgcccccg accgtcacct cgcggccgtc ggccgcctgc
     1081 cggagaccgt cggcggtgaa ggtcgtgaac gacttcaact cgtcgcggta ggcgtcgagg
     1141 ggatggccgg agatccagaa gccgagcatg gtcttttcct ggttgaggcg ttcggcgcgc
     1201 ggccattcct cgaggggcgg cagagcgggg gggacccgcc gcgcgccggc gctgtcggcg
     1261 aacaggtcgt gggaagcggc ggcttcctgg actttatggg cgaactcgag cacggcctcg
     1321 acggccgcgt acttctgcgc ccggttgccg gggagggaat cggccgcccc ggcggcgacg
     1381 agggattcca ggacgcggcg gttgagcgtc ttggccggca cccgggtcac caggtcggcc
     1441 aggtcgctgt agaggccgtc ccgccggcgt tcctcgacaa tcgccagcgc cggcgagccg
     1501 cccacgttct tcaccgcctg gaggccgaac cggatcttcc cctcggctac ggaaaaatcg
     1561 acctgggaat ggttgacgtc gggcgggagc acgtcgatgt tcgcgcggcg gcactcttcg
     1621 aggaaggcgt agatgcggtc ggagtcgttg atttccgagg tcatgagggc cgccatgaac
     1681 tctttcgggt agtactgctt gagccaggcg gtctggtagg cgacgtaggc gtagcaggtg
     1741 gagtgggcct tgttgaagcc gtagcgggcg aaggtctcga tctggtgaaa gacctcctcg
     1801 gagacgctgc gcgggacgcc caggcggtcg gccccctcga ggaactcctg tttctgggcg
     1861 gccatgagag cggcgtcctt cttccccatc gcc
//
LOCUS       DAOOKS010000428         1889 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_77571_length_1889_cov_4.322246, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000428 DAOOKS010000000
VERSION     DAOOKS010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77571_length_1889_cov_4.322246"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..527)
                     /locus_tag="PK112_07110"
     CDS             complement(3..527)
                     /locus_tag="PK112_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11853.1"
                     /translation="MSMRQEILDLAARTRSFSQRIARETVTGAPSAVAPLLVDMVRRG
                     QLVRVGFGAYAAPDRAEEARQYGTDGPVAQAILRALSKSPNLQMVELKRVAHAHSGGE
                     TVAALVAAMVGDGLLSLTTRGYALTSKGAKRIPSAPPVMPEMRPYRPPQMPPRRPGAM
                     DFVRAPSVYSGAPV"
     gene            complement(1075..1236)
                     /locus_tag="PK112_07115"
     CDS             complement(1075..1236)
                     /locus_tag="PK112_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPC11854.1"
                     /translation="MTEAKLREVHEATGVPMPTLAKIRYGVTADPRGSTLDKIRAYLR
                     CHPPISEAA"
     gene            1443..>1889
                     /locus_tag="PK112_07120"
     CDS             1443..>1889
                     /locus_tag="PK112_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004736779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HPC11855.1"
                     /translation="MANIEIRHRSTGAVLYAGDGESVCEVVAAAVQGGADLYGADLRG
                     AGLRGANLGEAKLGGADLGGANLGEAKLGGADLRGADFGGADLRGADLRGTNLGGADL
                     RWADLGGAGLRGAGLRGADLRGACLRGADLRGANLGEAKLGGADLGG"
BASE COUNT          306 a          652 c          662 g          269 t
ORIGIN      
        1 gctcataccg gcgcccccga atacacgctc ggcgcgcgca cgaagtccat cgcccccggc
       61 ctgcgcggcg gcatttgagg cggtcggtac gggcgcattt ccggcatcac gggcggcgcg
      121 ctcggaatgc gtttcgcgcc cttcgacgtg agcgcatagc cccgcgtggt cagactcaac
      181 agcccatcgc cgaccatcgc cgcgaccagt gccgccacgg tttcgccgcc cgagtgggcg
      241 tgagcaacgc gcttgagttc gaccatctgg aggttcggcg acttcgacag cgcccgcaga
      301 atcgcctgtg cgaccggacc atcggtgccg tactggcggg cctcctcggc cctgtccggc
      361 gcggcatagg cgccgaatcc gacgcgcacg agctggccgc gccgcaccat gtccaccagc
      421 agcggcgcga ccgcagacgg tgcacctgtg accgtctcgc gcgcgatccg ctgcgagaac
      481 gagcgagtgc gcgccgcgag gtcgaggatt tcttggcgca tgctcatgcc gcccccttca
      541 gcgcccgcag ctgctcctcg agctcgcgga tgcgcgcctg attcactgcg ccgatgagcg
      601 cgagtgcgga ttgcgagtgc gttccaaccg ttgcttccgg tttccactcg gctgcactga
      661 gactgtgacc catgacaacg actcccatcg tcagttcaga aaacgtgctt tgccgagcgc
      721 accgccggct cgcccgcagc gggggagtcg gccgacacga ggtcgcaagc gatgcgctcg
      781 aaaaagccgc ctgcgtgaca ggcggaaccg gacagggctt ccgggacagg aggaccaccg
      841 agaaagcgca aaagggtgcc cggttgcgca gttgcccgcc gggcgcgggc tgtggcccca
      901 cgaggccttc ccagagagcg cgccaccaaa gaacgcgatc cggcccgact gcgcgcgggc
      961 gaattcacag aagccgctcc aggcggtcga ggagctttgc gcggcggcgc tcgcgcagtg
     1021 ccagccggcc ggcgagcacg cagacggcta cacagaagat tgcggcggcg agtgtcatgc
     1081 tgcctcgctg atcggcggat gacagcgcaa gtacgcgcgg attttgtcca gcgtactgcc
     1141 acgcggatcg gcagtaacgc cgtaccggat cttcgccagc gtcggcatcg ggactccggt
     1201 cgcctcgtgc acctcgcgga gcttggcttc cgtcatctgc ctaaccgctg cgatcacttc
     1261 atctgatgtg ttcatgcgga tgaactctac cggattcgga atctgggcgc aactacaaac
     1321 ggtagcgtca atctggaaca gtgtcgggat ggacgacggt agacaacaga ttccgcattc
     1381 ggtaggattc tcccatcgcc ccaacgcacc cggcatccgc cggcaggggc agggaggaca
     1441 cgatggcgaa tatcgagatc aggcaccgga gcaccggcgc tgtgctgtac gccggcgatg
     1501 gcgagtcggt atgcgaagtg gtcgcggcgg ctgtgcaagg cggggccgac ctctacgggg
     1561 ccgacctccg cggggcgggc ctccgcgggg ccaacctcgg cgaggcaaaa ctcggcgggg
     1621 ccgacctcgg cggggccaac ctcggcgagg caaaactcgg cggggccgac ctccgcgggg
     1681 cggatttcgg cggggccgac ctccgcgggg cggacctccg cgggaccaac ctcggcgggg
     1741 ccgacctccg ctgggccgac ctcggcgggg cgggcctccg cggggcgggc ctccgcgggg
     1801 cggacctccg cggggcgtgc ctccgcgggg cggacctccg cggggccaac ctcggcgagg
     1861 caaaactcgg cggggccgac ctcggcggg
//
LOCUS       DAOOKS010000429         1888 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_77659_length_1888_cov_2.912711, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000429 DAOOKS010000000
VERSION     DAOOKS010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77659_length_1888_cov_2.912711"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="PK112_07125"
     CDS             <1..783
                     /locus_tag="PK112_07125"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HPC11856.1"
                     /translation="LLFLAAFLPAAAVRPAEPPVDSMSIGERFHFLTGFGDEGYRQKE
                     PAWGKLIPLYKSYEGAPRYRLPAPRQGALTVDEAIRTRRSERRFRPDPIPLPALAGLL
                     VSADGLTAGGRTERRAAPSGGALYPIETYVVVTGGDSLPDGLYHFQAADSSLALLQEG
                     DFRAALHGAAHEQEAVGSSPVTFLFTARFERSTQKYADRGYRYVYMECGAICENLYLQ
                     AAALGLGTVAVGSFNDDALNRFLGVDGRAEAALLMMPVGFPE"
     gene            958..1030
                     /locus_tag="PK112_07130"
     tRNA            958..1030
                     /locus_tag="PK112_07130"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:991..993,aa:Thr,seq:cgt)
     gene            1167..1871
                     /locus_tag="PK112_07135"
     CDS             1167..1871
                     /locus_tag="PK112_07135"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HPC11857.1"
                     /translation="MKWPLPKPKVTEQSPRLFQNDLVEMLSRTSPGVVVAIYLPTALG
                     LLWYSVARGGVPVPTTVLVAAVGAAAWTLTEYWLHRAIMHWVPNNGWGARMHFWIHGI
                     HHEWPKDPYRLVMPPSVSLVLFFLFLALWTAVFGRSGWAFHGGFTLGYLAYDLSHYFY
                     HHRKPEREWLVRLQRHHLRHHFHPRYEERNFAITVPWWDRIFGTATPAEPSGPVPGAA
                     RARSSAAEPGLRAGER"
BASE COUNT          260 a          626 c          631 g          371 t
ORIGIN      
        1 ttgttgtttt tggcggcgtt cttgccggcg gccgcggtcc gcccggccga gccgcccgtc
       61 gactccatgt cgatcggcga gcggttccat ttcctcaccg ggttcggcga tgagggttac
      121 cgacagaaag agccggcgtg ggggaagctg attcctctgt acaagtccta tgaaggggcg
      181 ccccgctacc ggcttcccgc accgcgtcaa ggcgcgctaa ccgtggatga ggcgatccgc
      241 acccgtcggt ccgagcgtcg tttccgcccg gacccgatcc cgcttcccgc gctcgcgggg
      301 ctgctggtgt cggccgacgg tctcacggcg ggcgggcgca ccgagcggag ggccgcgccc
      361 tccgggggcg cgctctatcc gatcgagacg tacgtcgtgg tgaccggcgg ggattcgctg
      421 cccgacggtt tgtaccactt ccaggcggcg gacagttcgc tcgcccttct ccaagagggg
      481 gatttccgcg cggcgctgca cggggcggcc catgagcaag aagcggtcgg ctcgtcgccg
      541 gtgacctttc tgttcaccgc ccgctttgag cgctcgaccc agaagtacgc cgaccggggc
      601 taccgctatg tctacatgga gtgcggagcc atttgcgaga acctctatct ccaggcggcg
      661 gcgctcgggc tggggacggt cgccgtgggt tcgttcaacg acgatgcgct caaccggttc
      721 ctcggggtcg acggccgcgc ggaggccgcg ctcctgatga tgcccgtcgg ctttcccgag
      781 tgacgcgccg aagcggggtc gggttgcgcg cgcccgggcg gcttccggcc ggagtcggcg
      841 cggtcctcgg gtgcgccaaa ttctcaagaa aatctcttga taaactccgg cccgccccta
      901 tatttgccga tcagaacgga ggggcgccgg tgcgcctccg ttgcaccaga ttgccaagcc
      961 accgtagctc agctggtaga gcaactgatt cgtaatcagt aggtcacccg ttcgattcgg
     1021 gtcggtggct tttctcttgt cgggcggggt ctcctgaacg ggaacccggc ggaaagtccg
     1081 ccggtttact tcacgacatt ttgccgcgcg gctctcggtg ccgatcgcgc cagcgacaac
     1141 ttctcggagt tttcggagtc ggtatcgtga aatggccctt gcccaaaccg aaagtcacgg
     1201 agcagtcccc ccggctgttt cagaacgatc tggtcgagat gctctcgcgg accagtcccg
     1261 gcgtcgtcgt ggcgatctac ctgccgacgg cgctggggct gctgtggtac agcgtcgcac
     1321 ggggcggcgt cccggtcccg acgacggtcc tcgtggcggc ggtcggtgcg gcggcctgga
     1381 cgctcaccga gtattggctc catcgtgcga tcatgcactg ggtgcccaac aacgggtggg
     1441 gcgcccggat gcatttctgg attcacggca ttcaccacga gtggcccaaa gatccgtacc
     1501 gcctcgtcat gcccccgtct gtcagcctcg tcctgttttt cctctttctc gcgctctgga
     1561 cggcggtgtt cggccgttcc ggctgggcgt tccacggcgg cttcacgctc ggctaccttg
     1621 cgtacgatct gtcgcactac ttctatcatc atcggaaacc ggagcgcgaa tggctggtgc
     1681 gcctgcagcg gcatcacttg cggcaccatt tccatccccg ctatgaagag cgcaatttcg
     1741 ccatcacggt gccgtggtgg gatcgcatct tcggcaccgc cacccctgcg gagccgagcg
     1801 gtccggtgcc gggggcggcc cgcgcgaggt catcggccgc cgaaccgggc ttgcgggccg
     1861 gcgagcgttg acttcccctg cggtgcgg
//
LOCUS       DAOOKS010000430         1888 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_77668_length_1888_cov_2.507911, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000430 DAOOKS010000000
VERSION     DAOOKS010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77668_length_1888_cov_2.507911"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..921)
                     /gene="hemC"
                     /locus_tag="PK112_07140"
     CDS             complement(4..921)
                     /gene="hemC"
                     /locus_tag="PK112_07140"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HPC11858.1"
                     /translation="MTDARPLIIGSRGSDLALWQAHFISDILARELGQTVEIKIIKTA
                     GDRIQDLSFDKMEGKGFFTKEIEEALLAGDIDLAVHSLKDLMTTQPAGLKLAAAGYRA
                     DRRELLLIRPAAHHGDGLIPVREGSVIGTSSARRKCQIAFHRPSARIKDLRGNVPTRI
                     AKLRDGQYDAIVLALAGVERLRLDLSGLVALPLDPRQFLPAPAQGILGIQIRTHDPRV
                     EAAAGRLGSPEMMLTARVERGLLQRFGLGCSLPLGVYSERQGDAFRLLAVLGTQRDGA
                     WTGLKRAEAAGPANDPEQIVEAAYTKLKE"
     gene            complement(918..>1888)
                     /locus_tag="PK112_07145"
     CDS             complement(918..>1888)
                     /locus_tag="PK112_07145"
                     /inference="COORDINATES: protein motif:HMM:NF013641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11859.1"
                     /translation="LFRTVASLDSLVVGETQITGQFKEAAREATEAGLSGTMLDVLTK
                     EALAVAKRVRTETSLGDGAVSMASLAYSELEAALGDRRERPTVALVGAGAMTSKMARY
                     IAKAGLAELVFVNRTADKAASLAREFGGRGMGLAEFLTTPPPVDAILSATASPAFVFT
                     HEHLEKLSAGRPPVLCIDLAIPQDFSPEFGRDPRAVLVDSPALKAREQRNLRRKFVES
                     SRANEIVGEAVQAFLRNRLEVSMKPILRDSYEQSLELANRALDDFFEKKLCELSREQK
                     EHLRTLVIKLIGHSSFGPARLLSNHLVDLQDQFLFDMLGESRKEAV"
BASE COUNT          326 a          624 c          611 g          327 t
ORIGIN      
        1 cgctcactcc ttcagcttcg tatatgcggc ctcgacaatt tgttccggat cgttggccgg
       61 tccggccgcc tccgcccgtt tcagaccggt ccaggcgccg tcccgctgcg tcccgagcac
      121 cgccagcagc cgaaacgcgt ctccctgccg ctcggagtac acgcccagcg gcagcgagca
      181 ccccagtccg aaccgctgga gcaggccgcg ttccacccgg gccgtgagca tcatctccgg
      241 agagccgagc cggcccgccg ccgcttccac ccgcgggtcg tgcgtgcgga tctggatgcc
      301 gagaattcct tgggcgggtg cgggaaggaa ctgccgggga tccaggggca gcgcgaccag
      361 gccggagaga tcgaggcgga ggcgctcgac cccggcgagc gcgagcacga tcgcatcgta
      421 ctgcccgtcc cgcagcttgg cgatccgggt cggcacattt ccgcgaagat ccttgatgcg
      481 cgccgagggg cggtggaacg cgatctggca cttgcgccgg gccgatgagg ttccgatcac
      541 gctcccctcc cgaaccggga tgagaccgtc gccgtggtgc gccgccgggc ggatcaggag
      601 aagttcgcgg cgatcggcgc ggtacccggc ggcggcgagc ttgagccccg cgggctgggt
      661 cgtcatgagg tctttgaggg agtgcacggc cagatcgatg tcgccggcca ggagcgcctc
      721 ttcgatctcc ttggtgaaga agcccttccc ctccatcttg tcaaaggaca ggtcctgaat
      781 acggtcgccg gcggttttga tgatcttgat ctccaccgtc tgacccaact cgcgggcgag
      841 gatatcggaa atgaaatggg cctgccagag agccaggtcc gagccgcgcg atcctatgat
      901 gagaggcctt gcgtcggtca aaccgcttcc ttacgacttt cccccaacat atcaaacagg
      961 aactgatcct gcaagtcgac aagatggttt gacaggagac gcgcggggcc gaaagacgag
     1021 tggccgatga gttttattac aagagtgcga aggtgttctt tctgttcgcg ggagagttcg
     1081 caaagcttct tctcaaagaa atcatcgagt gcccggttgg ccagttcgag cgactgttcg
     1141 tagctgtcgc ggagaatcgg cttcatcgat acctccagcc gatttcgcag aaacgcctgc
     1201 accgcctcac cgacaatctc attggcccgg ctcgactcga caaacttgcg gcgcaggttg
     1261 cgctgctcgc gggctttaag cgcggggctg tccaccagca ccgcgcgggg atcgcggccg
     1321 aactcggggg agaaatcctg cgggatcgcc aggtcgatac agagcaccgg cggccggccc
     1381 gcggagagct tctccaggtg ctcgtgggtg aacacgaatg cgggggaagc ggtcgcggag
     1441 aggatcgcgt cgacgggcgg gggagtggtg aggaactccg ccagtcccat cccccggccg
     1501 ccgaactcgc gcgccagact cgccgccttg tccgccgtcc ggttgacaaa gaccagctcc
     1561 gccaggccgg ccttggcgat gtaacgggcc atcttcgatg tcatcgcgcc cgccccgacc
     1621 agcgccacgg tcggtcgctc cctccggtcg cccaacgccg cttccagttc ggagtacgcc
     1681 agcgaagcca tcgacacggc gccgtcgccg agactggtct cagtccgtac ccgcttggcg
     1741 accgcgagtg cctctttcgt caacacgtcg agcatcgttc ccgacaaccc cgcctcggtc
     1801 gcttctcggg cggcctcttt gaactgaccg gtgatctggg tttctcccac gacgagcgag
     1861 tcaagggagg ccacggtgcg gaacaagt
//
LOCUS       DAOOKS010000431         1887 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_77724_length_1887_cov_2.472707, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000431 DAOOKS010000000
VERSION     DAOOKS010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77724_length_1887_cov_2.472707"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1169)
                     /locus_tag="PK112_07150"
     CDS             complement(<1..1169)
                     /locus_tag="PK112_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11860.1"
                     /translation="MSLPRSAGRGILGPGALLLVLAAGCSSGLNREKFYAPLEADLRS
                     GNYAAAVATVEEARSKGAYKEKDRFLYYLDAGLAYHYASQFDSSNLRLAVAERAAEEL
                     FTKSISKGVLANTLLNDNVLEYPGEDYEVLYTNLLMALNYLALGKHDDAFVEIRRAHG
                     KLNLLEQKYADAAAAIAADAARDTTGACVAFAGTDVRFNSSAFARYLSMHMYAAEGLD
                     DDARIDSQALADAFARQPNIYAFPPPPVAYAADSGGVLSVVGLVGLAPVKQPLNLRVR
                     TDENLHLLTVIYDDARPEHTVFANYPLPEGIGDFYAKLSLPQLVPRPSEIAAVRVSSG
                     GRVLGELQLLEDVAAVAAASFEARQSIILWRTVVRVVLKTLSASRLKNAVDNGGIA"
     gene            complement(1166..1768)
                     /locus_tag="PK112_07155"
     CDS             complement(1166..1768)
                     /locus_tag="PK112_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008283261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HPC11861.1"
                     /translation="MTMRYLLVVLALAALAIGCGGGQTVTRLDPGGTTDLSGKWNETD
                     ARLVAEEMIADALARPWLMNFVEKNGEKPVVTVGTIRNKTNEHIDTEVFTADFERELI
                     NSGNVRFVGSHDERYEVREERQDQAEFASEETRKLLHDELGADFILLGAIKTITDAVE
                     GRSTVFYQTDLELINIETNEKVWIGTKKIKKGIAQSGTKW"
BASE COUNT          305 a          589 c          645 g          348 t
ORIGIN      
        1 gcgatcccgc cgttgtcgac ggcgtttttc agtctggagg cggagagggt tttgagcacg
       61 acccgcacga cggtgcgcca gagaatgatc gactggcggg cttcgaaaga ggcggccgcg
      121 accgccgcga catcctcgag caactgcagc tcgccgagca cccggccgcc cgaggagacc
      181 cggacagccg cgatttccga cggtctcggg accagttggg ggagcgacag tttcgcgtag
      241 aagtcgccga tcccctcggg cagcgggtag ttggcgaaca ccgtgtgctc cgggcgggcg
      301 tcgtcgtaga tcaccgtcag caggtgcagg ttctcatcgg tgcggacgcg caaattgagc
      361 ggctgcttca ccggcgcgag acccaccagc ccgaccacgc tgagcacgcc gccgctgtcc
      421 gcagcgtagg cgaccggagg cggagggaac gcgtagatgt tgggctggcg cgcgaaggca
      481 tcggccagcg cctgcgagtc gatccgcgcg tcgtcgtcga gcccctcggc ggcgtacatg
      541 tgcatcgaca ggtagcgggc gaaagcggag ctgttgaaac gcacatccgt gcccgcgaaa
      601 gcgacgcagg cgccggtcgt gtcgcgggcg gcgtcggccg cgattgccgc ggccgcatcc
      661 gcgtacttct gttccagcag gttgagcttg ccgtgggcgc ggcggatttc cacgaacgca
      721 tcgtcgtgct tccccagggc aaggtagttg agggccatga ggaggttcgt gtagagaact
      781 tcatagtcct cgccggggta ctccaggacg ttgtcgttga gcagagtgtt ggcgagcacc
      841 cccttggaga tactcttggt gaagagttcc tcggcggccc gctcggcgac cgcgaggcga
      901 aggttggagg agtcaaactg ggaggcgtag tggtaggcca gaccggcatc gaggtagtac
      961 aggaagcggt ccttctcctt gtaggcccct ttcgatcggg cctcctcaac ggtggcgacg
     1021 gcggcggcgt agttgcccga gcgcagatcg gcctcgagcg gcgcgtagaa cttctcccgg
     1081 ttgaggccgg aggagcagcc ggccgcgagc accagcaaaa gcgcgcccgg acccaggatc
     1141 ccccgccccg ccgatcgcgg caggctcacc atttcgtgcc ggactgggca atccccttct
     1201 tgatcttctt cgtcccgatc cagactttct cgttggtctc aatgttgatg agttcgaggt
     1261 cggtctggta gaagacggtg ctccggccct cgaccgcatc ggtgatggtc ttgatcgcgc
     1321 cgagcaggat gaagtcggcg ccgagctcat cgtggaggag tttgcgggtc tcctcggagg
     1381 cgaactccgc ctggtcctgc cgctcctcgc gaacctcgta gcgctcatcg tggctgccca
     1441 cgaagcgcac gttgccggag ttgatcagtt cgcgttcgaa gtcggcggtg aacacctcgg
     1501 tgtcgatgtg ctcgttggtt ttgttgcgga tggtgccgac agtgacgacc ggcttttcgc
     1561 cgtttttctc gacgaaattc atcagccagg ggcgggcgag cgcatcggcg atcatctcct
     1621 cggccacgag gcgcgcatcg gtctcgttcc acttgccgct gaggtcggtg gtgccgcccg
     1681 gatcgaggcg ggtcacagtc tggccgccgc cgcagccgat cgccagcgcc gccagcgcca
     1741 gcacaactaa caggtatctc atagtcatct cctctgccgc cacgttgcgg cctatctggt
     1801 agtaagggtt accggcactc gcggtccggc aggaagccga cccggcttcc ccggaacatc
     1861 gtttcatatt ccgggacggg cttgccg
//
LOCUS       DAOOKS010000432         1881 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_78055_length_1881_cov_3.642388, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000432 DAOOKS010000000
VERSION     DAOOKS010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78055_length_1881_cov_3.642388"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="PK112_07160"
     CDS             complement(<1..244)
                     /locus_tag="PK112_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HPC11862.1"
                     /translation="MEPLQIVVIGAIGAALVFDIINGFHDAANSIATVVSTRVLNPRL
                     AVLWAAFFNFTAMFVFVPRVADTVSKIVRIDGKDTVY"
     gene            complement(249..878)
                     /locus_tag="PK112_07165"
     CDS             complement(249..878)
                     /locus_tag="PK112_07165"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HPC11863.1"
                     /translation="MLEKLLPRETSFFKFFEQTSGLAIEVCVELDNLVNSSNDVAGRV
                     ARIEQLEHDADEVTHQCIDALHATFITPIDRADIHRLIKRIDDIIDCVDSAAARFQLY
                     GLTVMRPEIRQLTQVLIASVRELDKAVRDLPALNRKAEAIQECCQAVYEAEREADDLL
                     RAALVRLFAEEKDVFLVIKWKEMFERVERATDRCQEVANIISGIVIEAS"
     gene            complement(1207..>1881)
                     /locus_tag="PK112_07170"
     CDS             complement(1207..>1881)
                     /locus_tag="PK112_07170"
                     /inference="COORDINATES: protein motif:HMM:NF015882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HPC11864.1"
                     /translation="GDPADAYQAEFRQPREVAADWPAPKDRAPNLQEVIRHGRITTLI
                     GLSGQKGAFTPEILAALAANAPRPVILALSNPTSCCETTPEAVVAATGGRGIIATGSP
                     SRPVTLPDGREMTVSQCNNLYVFPGMGLGAIVCQAQKVSHRMFHAATCAISDMVTEEM
                     RSTGCLLPALENAREISFAVALAVARQAHDEGIGLAASDDRLAALIRGAMWEPKYYPY
                     RADMGH"
BASE COUNT          327 a          616 c          605 g          333 t
ORIGIN      
        1 catacacggt gtctttgccg tcgatgcgca cgatcttgga caccgtgtcg gccacgcggg
       61 ggacgaagac gaacatggct gtgaagttga agaaagcggc ccagaggacc gccaggcgcg
      121 ggttgagcac gcgcgtcgac acgaccgtcg caatcgagtt cgcggcgtcg tggaatccgt
      181 tgatgatatc gaaaaccagc gccgcgccga ttgccccgat gacgacgatc tgaagcggtt
      241 ccatcgactc aggatgcctc gataacgatg cccgagatga tgttcgcgac ttcctggcaa
      301 cgatcggtcg cccgctccac ccgctcgaac atctccttcc acttgatcac caggaacacg
      361 tccttttcct ctgcgaacag gcgcacgagg gccgcccgca agaggtcgtc ggcctcccgc
      421 tccgcctcgt acactgcctg gcagcattcc tggatcgcct cggccttacg gttgagcgcg
      481 gggagatcgc gcaccgcttt gtcgagctcg cgcaccgagg cgatcaggac ctgggtgagc
      541 tgccggattt ccggccgcat cacggtcaga ccgtaaagct gaaaccgcgc cgcggccgag
      601 tcgacgcagt cgatgatgtc gtcgatgcgc ttgatcaggc ggtggatatc ggcccgatcg
      661 atcggggtga tgaaggtggc gtgcagcgca tcgatgcact ggtgcgtcac ctcgtcggcg
      721 tcatgctcca gttgctcgat ccgcgccacc cggccggcaa cgtcgttgga cgaattcacc
      781 aggttgtcca gttcgacgca cacttcgatc gccagcccgc tcgtctgctc gaagaactta
      841 aagaagctgg tttctctggg aaggagtttc tctaacatgg catctcccgt ctgagacgcc
      901 cggtcatggt ccggcggcgt cgttcgagga tattccccgg tcacaaatca ggggcaatct
      961 taacacgatc cgaaccatcg ggcaagcgat ttaagcggaa atattgcccc gtcggtcgcc
     1021 gctcggccgg tcgacccggg cgtcgcggcc gcgaataacc gaaagccaat aaggaggttg
     1081 accggtcaag tgtccgccgg ggccggccgg ccaagtgtga tcccgctccc ccgtcccgaa
     1141 ataggaccgg cccgcgcgga gaggcgggcc ggtgagtaat tgcaggttcg tgtgtcgaag
     1201 tttacgctaa tgccccatgt ctgcccggta ggggtagtac ttcggctccc acatggcgcc
     1261 gcggatgagg gctgcgaggc ggtcatcgga ggcggccagg ccgatgcctt catcgtgcgc
     1321 ctgccgggcc accgccagcg ccaccgcgaa cgagatctcg cgggcgtttt cgagtgcggg
     1381 cagcagacac ccggtcgacc gcatctcctc ggtgaccatg tccgaaatgg cgcaggtggc
     1441 ggcgtgaaac atgcggtgcg aaaccttctg cgcctggcag acgatcgccc cgagacccat
     1501 gccggggaag acatacaggt tgttgcactg cgacaccgtc atctcccgcc cgtccggcag
     1561 cgtcaccggc cgactggggc tccccgtggc gatgatgccc cgtcctccgg tcgccgccac
     1621 caccgcctcc ggggtcgttt cgcagcacga ggtcgggttg gacagcgcca ggatgaccgg
     1681 ccgcggagca ttcgccgcca gcgccgcgag gatctccggg gtgaaagctc ccttttgtcc
     1741 cgacagaccg atgagggtcg tgatccgtcc gtgccggatg acctcctgga gattgggcgc
     1801 ccggtccttg ggcgccggcc agtccgccgc gacctcgcgg ggctgccgga actccgcctg
     1861 ataggcgtcc gccgggtcgc c
//
LOCUS       DAOOKS010000433         1879 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_78183_length_1879_cov_4.478618, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000433 DAOOKS010000000
VERSION     DAOOKS010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78183_length_1879_cov_4.478618"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="PK112_07175"
     CDS             complement(<1..288)
                     /locus_tag="PK112_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPC11865.1"
                     /translation="MGQSALTFHPLTPERWGDFEALFGVRGACGGCWCMYWRLFRLAF
                     SIGKGEGNRRAMQKLVRAGTEPGILAYDGERPVGWCAVAPREEYVRLTAART"
     gene            complement(444..740)
                     /locus_tag="PK112_07180"
     CDS             complement(444..740)
                     /locus_tag="PK112_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HPC11866.1"
                     /translation="MKTFFLFTKLAPSDGKRMRERQSLGRAWLHEVKEKCPDVKFLSH
                     YALLGDYDFVDIYEAPDEETAMKVSMISMDKGAFQAKSVLAIPYARLVELANEL"
     gene            complement(873..1352)
                     /locus_tag="PK112_07185"
     CDS             complement(873..1352)
                     /locus_tag="PK112_07185"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPC11867.1"
                     /translation="MGITINPVAESDRPWVEEVFRSWGADFVVTRGRKVCPEETEGLY
                     AADERGRRIGLLTFQIAGTQCEVVTLDAFEKFRGIGTALMDRAVALARARGCTRVWLI
                     TTNDNLDAIRFYHRRGLVIAHVHVGAITESRRLKPSIPLLGAYGIPIRDEIEFELVL"
     gene            complement(1368..>1879)
                     /locus_tag="PK112_07190"
     CDS             complement(1368..>1879)
                     /locus_tag="PK112_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPC11868.1"
                     /translation="AHSRRLNPGVEHFLGDMRTVRLGRTFDAVVIHDAIAYMLSGDDL
                     RAAFATAAAHLGTGGVFVTSPDYVAETFVDGSIACETRPFDGGSFTYVEYQWDPDPSD
                     TQTETVFTYIIRRGGKVQIEQDRHLFGLFPLRTWESLMAQAGFAVARRDYPVRDDGQP
                     GWLLIGRKL"
BASE COUNT          320 a          679 c          578 g          302 t
ORIGIN      
        1 cgtgcgcgcc gccgttagcc ggacatactc ctcccggggc gccaccgcgc accagccgac
       61 cggccgctcg ccgtcgtagg cgagaatgcc cggttccgtt cccgcccgga cgagtttctg
      121 catggcccgg cggttgccct cccccttgcc gatcgagaat gcgaggcgga acagccgcca
      181 gtacatgcac cagcatccgc cgcaggcgcc acgcacgccg aagagggcct caaaatcacc
      241 ccatcgctcc ggagtgagcg ggtgaaacgt gagagcggat tgtcccatcg gtggtttact
      301 cacaggtcac cattcacgac cgccgggacc gccgcagtcc cggccggaga atgctttcgt
      361 ccgcgcccgc aagtcgattt cccgatgctc cgacgcaccc ggtcgcggcg ctcggcccgg
      421 cgacggggtt ctccccctcc gcctcacaac tcattggcca gttccaccag gcgggcgtag
      481 gggatcgcca ggacgctctt ggcctggaac gcccccttgt ccatgctgat catcgagacc
      541 ttcatcgccg tctcttcgtc gggcgcctcg tagatgtcga cgaaatcata gtcgccgagg
      601 agcgcgtagt gggagaggaa tttcacgtcc gggcacttct ccttgacctc gtggagccac
      661 gcccgcccga gcgactgccg ctcgcgcatc cgcttgccgt ccgacggcgc gagcttggtg
      721 aacagaaaga acgttttcat ggagtcctcc ttcgcaatcg agatgcggcc ggattcccgc
      781 cgccgcaccg tccgtccgcc ggcgatcccg cgggcaaccg gcgtccgtag tatacgcacc
      841 cggcgggccg ctgtaaagag cccctggccg ggtcagagaa ccagctcgaa ctcgatctcg
      901 tcccggatcg gaatcccgta ggcgccgagg agcgggatgc tcggtttgag ccgacgcgac
      961 tccgtgatgg ctccgacatg cacatgggcg atcaccaacc cccgccggtg gtagaagcga
     1021 atcgcatcga ggttgtcgtt ggtcgtgatc aaccagacgc gggtgcaccc acgcgcgcgg
     1081 gcgagagcga ccgcgcggtc catcagggcc gtgccgatgc cccggaactt ctcgaacgcg
     1141 tcgagcgtca cgacctcgca ctgcgtcccg gcgatctgga aagtgagcag accgatccgc
     1201 ctcccccgct cgtcggccgc atacagcccc tcggtttcct ccgggcagac cttgcgcccg
     1261 cgggtgacga caaaatcggc cccccaggag cggaaaacct cctccaccca ggggcggtcg
     1321 ctttcggcca ccggattgat tgttatcccc atgcgctcct cgctggacta gagctttctc
     1381 ccgatcaaca gccatcccgg ctggccatcg tcacgcaccg ggtagtcgcg gcgcgcgacc
     1441 gcgaacccgg cctgcgccat cagcgattcc caggttcgga gcggaaacag cccgaacagg
     1501 tgccggtcct gctcaatctg caccttcccg ccgcggcgaa taatatacgt gaacaccgtc
     1561 tcggtctgcg tgtcggacgg gtcgggatcc cattggtact cgacataggt gaacgacccg
     1621 ccgtcgaacg gccgcgtctc gcaggcgatc gacccgtcga cgaacgtctc ggccacgtag
     1681 tccggggagg tgacgaacac cccgccggtg cccagatggg cggcggcggt ggcgaacgcg
     1741 gcgcgcagat cgtccccgct cagcatgtac gcaatcgcat cgtggatcac cacggcatcg
     1801 aacgtccgcc ccagccgcac cgtgcgcatg tctccaagga agtgctccac ccccgggttg
     1861 aggcggcgcg agtgcgcca
//
LOCUS       DAOOKS010000434         1879 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_78202_length_1879_cov_2.966557, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000434 DAOOKS010000000
VERSION     DAOOKS010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78202_length_1879_cov_2.966557"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="PK112_07195"
     CDS             <1..360
                     /locus_tag="PK112_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11869.1"
                     /translation="VFGVALGTAIPQVVIYSFVYPYVFHRTIRARLSDFYRDSGLSIA
                     TALLFTLPVAWAVSYLPLPGWVGFLAQISAVTAVSLAGFAWRILDDADRRRFLGRFFR
                     SRAAGPQPPEKKTAPGR"
     gene            379..1728
                     /locus_tag="PK112_07200"
     CDS             379..1728
                     /locus_tag="PK112_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HPC11870.1"
                     /translation="MGLYNLFHSTLFRLKDLRDRTGVYRLAARLRREQFLPRPDLDDL
                     RRRRLLDLLAHARLHSPYYRDLLAGLEPDLERIAPHEILSRIPLLTREIVQEEGSRLL
                     CPGIPGSYPDSSGGSTGRPVNFHHDLTYLNWGRAPELFFLSWMGVRPGDRTAVFWGAD
                     RDLKEESLYTRLMLRLNRVRQLNSFAMSEAQIDAFLKQLNAFRPRYIYGYASSLYHVA
                     TFIARGRPLGFRPLAIRSSAEMLYPVQRAAIEQAFDAPVHNFYGSREVNNIAAECAAH
                     DGLHIFASARMVEIVDDRGRLLPPGEVGHIAVTDLTNRAFPFIRYLNGDLAALKSGLC
                     PCGRTYPRLEKIAGRSSDIITVEGRHIHGEYFTHLFYGRPEVKAFQLIQETEYDFRLL
                     LVPRTRDLDTAFFRDRIRDKLGPRVRLEIALVDDIPPTAGGKHRFTVSKVPPRKDGL"
BASE COUNT          289 a          747 c          485 g          358 t
ORIGIN      
        1 gtgttcggcg tcgcgctcgg cacggccatt ccacaggtcg tcatctactc cttcgtctac
       61 ccgtacgtct tccaccgcac tatccgcgcc cggctctcgg acttctaccg cgacagcggc
      121 ctttccatcg ccactgccct cctgttcacg ctgccggtcg cctgggcggt gtcctacctg
      181 ccgctgcccg gctgggtggg atttttggcg cagatttcgg ctgtcaccgc cgtctcgctg
      241 gccggcttcg cctggcggat ccttgatgac gctgaccgcc gccgcttcct cggccggttt
      301 ttccgctcgc gggcggctgg acctcaaccg ccggagaaaa agaccgcccc gggccgctga
      361 gcccgcgcgg gaaatcccat gggactgtac aacctcttcc acagcaccct cttccggctg
      421 aaagacctcc gcgatcgcac cggcgtgtac cgcctggcgg cccggctgcg ccgcgagcag
      481 ttcctccctc gaccggacct cgacgatctc cgccgccgcc ggctcctcga cctgctggcg
      541 cacgcccgtc tccattcccc ctactaccgc gacctcctcg ccggcctcga acccgatctg
      601 gagcgaattg ccccgcacga aatcctctcg cggatccctc tgctcacccg cgagattgtt
      661 caggaggagg gctcccgcct cctctgcccc ggcatcccgg gctcctaccc ggattcctct
      721 ggcggctcca ccggccggcc ggtcaatttc caccacgacc tgacctacct caactggggc
      781 cgcgcccctg agctgttctt tctctcgtgg atgggggtcc gccccggcga ccgcacggcc
      841 gtcttctggg gcgccgaccg cgacctcaag gaggagtccc tgtacacgcg tctcatgctc
      901 cgcctcaacc gtgttcgcca gctcaactcc tttgcgatgt ccgaggccca gatcgacgcc
      961 tttctcaaac agctcaacgc tttccgaccg cgctacatct acggctacgc ctcctccctg
     1021 taccacgtcg ccactttcat cgcccggggc cgccccctcg gcttccgccc cctcgccatc
     1081 cggtcttccg cggaaatgct ctaccccgtc cagcgcgccg ccatcgagca ggcgtttgac
     1141 gcccccgtgc acaatttcta cggctcgcgc gaggtcaaca acatcgcagc cgaatgcgcc
     1201 gcccacgacg gtctgcacat cttcgcctcg gcccgcatgg tcgagattgt cgacgaccgc
     1261 ggtcgcctcc tgccccccgg cgaggtcggc cacatcgcgg tcaccgatct gaccaaccgc
     1321 gcgttcccct tcatccgcta cctcaacggc gatctggcgg ccctgaaatc aggcctctgc
     1381 ccctgcgggc gcacctaccc ccggttggag aagatcgccg gccgaagctc cgacatcatt
     1441 acagttgagg gccgccatat ccatggcgag tacttcaccc atctctttta tggccgccct
     1501 gaagtcaaag cctttcaatt gattcaggaa acggaatacg actttcgact cctcctggtc
     1561 ccgcgcactc gggacctcga caccgccttc ttccgcgacc gcatccgcga caagctcggt
     1621 ccccgcgtcc ggcttgaaat cgctctcgtc gacgatatcc ccccgaccgc gggcggcaag
     1681 caccgcttca ccgtctccaa agtcccgccc cgcaaggacg gtctttgaaa ctgcattaga
     1741 acggcctata ttaaaggtcg gaacgaaaac gtccgaacag acggtataag cgactatggc
     1801 aaccacaacg catcatctcc ccggcggatc gcgggcgtac ctgcggcccg agcatctcgc
     1861 ctacgccgct ttcgccgcc
//
LOCUS       DAOOKS010000435         1868 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_78934_length_1868_cov_2.917816, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000435 DAOOKS010000000
VERSION     DAOOKS010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78934_length_1868_cov_2.917816"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1275)
                     /locus_tag="PK112_07205"
     CDS             complement(<1..1275)
                     /locus_tag="PK112_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HPC11871.1"
                     /translation="MKNPTPHPENARCDYRVDELVERARYMRGLNEIALCSARSGHSG
                     GTLSVMDIAAALYLRIARHNPTDPDWPDRDRIIWSAGHKAPALYTALAVAGYFPEPEL
                     MKLRMLDSPLQGHPHRLDLPGVEISSGSLGQGFSVAVGVALAARLDGKDYRVFVISSD
                     GEHQEGSMWEAAMAAAHYRLDNLILIVDKNRLQIDGPVAEVMNIDPLADKYAAFGWHV
                     READGHAMAEIVEALDWARNRNDSGKPVALIFHTVKGKGVSFMENVVGWHGKPPKLDE
                     LERLLAELGLGDTFAVSDLIDYGVRHQIGVDARLNDELPAFSRDFWWNAGPAMRVDMD
                     PTRKGFGRALEACGSDERIVCLGADISDSICISDFHKNHPERRRRFFWVGVAEQNATT
                     IAVGLAKEGKIPVFGTYGVFAAARNLDQIRVSV"
     gene            complement(1250..>1868)
                     /gene="coaE"
                     /locus_tag="PK112_07210"
     CDS             complement(1250..>1868)
                     /gene="coaE"
                     /locus_tag="PK112_07210"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HPC11872.1"
                     /translation="GLTGLIGAGKSTAARILESFGAVVLDADEIGREVVEASLPLRHR
                     LAEAFGADVLTVDLDLDRKKVAERAFAGEESRRRLNEIVHPYLLHELDSRMRRLAKRC
                     PVVVVDAALLTEWRLHQRMDYTIVIDAPEQVRLDRLAARGMAPSEARAIERLQPSPQD
                     LRVCADFLVVNDGSTDRLAATLSDIWEMITRSKRGDSHEEPDTAP"
BASE COUNT          302 a          586 c          670 g          310 t
ORIGIN      
        1 cacggagacg cggatttggt cgaggttgcg ggcggcggcg aacacgccgt aggtgccgaa
       61 gacggggatt ttcccctcct tggccaggcc gaccgcgatg gtcgtggcat tctgttcggc
      121 cacgcccacc cagaagaagc ggcggcggcg ctccgggtgg ttcttgtgga aatcggagat
      181 gcaaatggag tcggagatat cggcgccgag gcagacgatg cgctcgtcgc tcccgcaggc
      241 ctcaagagcg cggccgaacc ctttgcgcgt aggatccatg tccacgcgca tggccggccc
      301 ggcattccac cagaagtcgc gcgagaacgc ggggagttcg tcgttcaggc gggcgtcgac
      361 gccgatctgg tggcggacgc cgtagtcgat gagatcggac acggcgaagg tgtctccgag
      421 gccgagctcg gccagcaggc gctccagttc gtcgagcttc ggcggcttgc cgtgccaacc
      481 gacgacgttc tccatgaacg acacgccctt ccccttcacc gtgtggaaga tcagcgcgac
      541 cggcttgccg gagtcgttgc ggttgcgggc ccagtcgagg gcctcaacga tctcggccat
      601 ggcgtggcca tcagcctcgc gcacgtgcca gccgaaagcg gcgtatttgt cggccagcgg
      661 gtcgatgttc atgacctcgg caaccggacc gtcgatctgc aggcggttct tgtcgacgat
      721 gaggatcagg ttgtcgagac gatagtgggc ggcggccatg gcggcctccc acatcgagcc
      781 ttcctggtgc tcgccatcgg acgaaataac aaacacccgg tagtccttcc catcgagccg
      841 ggcggcgagg gcgacgccga cggcgactga aaacccctgc ccgagcgacc cggagctgat
      901 ctccaccccc ggcaggtcga ggcggtgggg gtgcccctgg agcggggagt cgagcatgcg
      961 gagcttcatg agctcgggtt ctgggaaata gccggcgacg gccagggcgg tgtagagagc
     1021 gggggctttg tggccggccg accagatgat gcggtcgcgg tcgggccagt cggggtcggt
     1081 tggattgtgg cgggcgattc tcaaatagag ggcggccgcg atatccatca cgctcagcgt
     1141 gccgccggag tgccccgacc gggccgagca gagagcgatt tcgttcaggc cgcgcatgta
     1201 gcgcgcgcgc tcgaccaatt cgtcgacgcg gtagtcgcag cgcgcattct cagggtgcgg
     1261 tgtcgggttc ttcatgcgag tctccccttt tcgagcgggt gatcatctcc cagatgtcgc
     1321 tcaaggttgc ggcgaggcga tcggtggatc cgtcgttgac gaccaggaag tcggcgcaga
     1381 cgcgcaggtc ctgcggggac ggctgcaggc gctcgatcgc ccgggcctcg gacggcgcca
     1441 tcccccgcgc ggccagccgg tcgagccgga cctgttcggg ggcatcgatc acgatcgtgt
     1501 agtccatgcg ttgatgcagg cgccactcgg tcagcagcgc ggcgtcgacc accaccaccg
     1561 ggcagcgctt ggccagccgg cgcatgcggc tgtcaagctc gtggaggagg taggggtgga
     1621 cgatctcatt caggcggcgg cgggactcct cgccggcaaa cgcgcgttcg gcgactttct
     1681 tgcggtcgag gtcgaggtcg acggtgagca cgtcggcccc gaaggcttcg gccagccggt
     1741 gccgcagtgg gagggaggcc tccaccacct cgcgcccgat ctcatcggca tcgagtacca
     1801 cggcgccgaa cgattcgaga atgcgggcgg ccgtcgactt ccccgcgccg atcaatccgg
     1861 ttaaaccg
//
LOCUS       DAOOKS010000436         1862 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_79300_length_1862_cov_3.264527, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000436 DAOOKS010000000
VERSION     DAOOKS010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79300_length_1862_cov_3.264527"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="PK112_07215"
     CDS             complement(<1..241)
                     /locus_tag="PK112_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11873.1"
                     /translation="MIRKELWLFLAKLVPLTLLLGYVWFRTVQRQYPDFVDVVADPFF
                     RLVGVRRWWLALVIEHFTNIVPYLALVLASPDLIAR"
     gene            complement(207..743)
                     /gene="xrtH"
                     /locus_tag="PK112_07220"
     CDS             complement(207..743)
                     /gene="xrtH"
                     /locus_tag="PK112_07220"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase H"
                     /protein_id="HPC11874.1"
                     /translation="MSDSRPGRSPALRFVLKFVLLLAVLGGAYAWLTARYAESLGWLM
                     DTTAAVTGSVVSLFFGEVHWSGRFVSYRGFPVEVIDECTGLLEMVIFTAAVLAFSTTP
                     GKKLLGLALGLPAIYLFNILRIVVLVIAGASSKPLFDFLHLYFWQATLILMIASVWIA
                     WIYLVVFRDPKRAVAVPG"
     gene            complement(864..1181)
                     /locus_tag="PK112_07225"
     CDS             complement(864..1181)
                     /locus_tag="PK112_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11875.1"
                     /translation="MRGDQALSQGVRRCHLTGRCAIAGNSGGSGSAADRPWWILYLSL
                     LVAGLLLIADGFHVAHLHRWTAKLGIALAYSAFVLFVGEGRTLAVVSAGLVCSAVLAT
                     FLW"
     gene            complement(1133..>1862)
                     /locus_tag="PK112_07230"
     CDS             complement(1133..>1862)
                     /locus_tag="PK112_07230"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HPC11876.1"
                     /translation="YDPRDPLIDYAAWSQPEEGLYELTIRLTKDLWGYDAYYRGSTLY
                     LQVYRAPAHVDDLKGKVIVLDPGHSRDPGSVGPTGLSEAEANLAIALELRDRLEGAGA
                     RVVMTRIDTAHVELADRPKIAAAAEADLFVSIHNNALPDGVNPLVNNGASAYYYHPHS
                     VNLARAIHDELVSRTGLADHGFFHGNLAVLRPTGYPAVLVECTFMILPEQEDALKTRA
                     FRVTLADALCEGIKRFLKEYADAT"
BASE COUNT          379 a          551 c          670 g          262 t
ORIGIN      
        1 agcgggcgat caggtccgga gacgccaaaa cgagcgccag gtacgggact atgttggtga
       61 agtgttcgat caccagggcg agccaccagc ggcgcacgcc caccagccgg aaaaacgggt
      121 cggcgaccac gtcaacaaag tccgggtact gccgctgcac cgtgcggaac caaacgtagc
      181 ccaaaagcag agtgagcgga acaagtttag ccaggaacag ccacagctct tttcggatca
      241 cgaaacacca ccaggtatat ccaggcgatc cagacggacg cgatcatgag aatcagggtc
      301 gcctgccaaa aatacaggtg caggaagtcg aacaacggtt tcgaggatgc gccggcgatc
      361 accagcacga cgatgcggag gatgttgaag aggtagattg ccggaagccc gagcgccagg
      421 ccgaggagct tcttcccagg cgtggtggag aaggcgagga cggcggcggt gaagatgacc
      481 atttccagca ggcccgtgca ttcgtcgata acctcgaccg ggaatccgcg gtaggagaca
      541 aagcggccgg accagtgcac ctcgccgaag aagagcgaga cgacgctgcc ggtgacggcg
      601 gcagtagtgt ccatcagcca tccgagactc tcggcatagc gggcggtcag ccaggcgtag
      661 gcgccgccga ggaccgccag caggaggacg aatttcagga cgaagcggag ggcgggggat
      721 cggccggggc ggctgtccga caaggacgcc ggggggcggt cgggcaatga cttcctgcgt
      781 gggcgtgcca tcaactgcaa tttagcccgc ggcggggagg ggcgcaacac ccgattgcgc
      841 ggccgccaag cccgggtcgc cgctcaccag aggaaagtgg ccagaaccgc ggagcagacc
      901 aatccggccg agacgaccgc gagcgtgcgg ccttccccga cgaagaggac aaacgcggag
      961 taggcgagtg cgatgccgag tttggccgtc cagcggtgaa ggtgggcgac gtggaaaccg
     1021 tcggcgatca gcagcagccc ggcgacgagc aggctgagat agaggatcca ccacgggcgg
     1081 tcggccgccg atcccgatcc cccgctgtta cccgctatgg cgcacctccc ggtcaggtgg
     1141 catcggcgta ctccttgaga aagcgcttga tcccctcgca cagagcatcg gccagggtca
     1201 cccgaaacgc gcgggtcttg agggcatcct cctgctcggg gaggatcatg aacgtgcatt
     1261 cgaccaggac ggccgggtag ccggtcgggc gcagcacggc gaggttgccg tggaagaatc
     1321 cgtggtcggc caggcccgtc cgactgacca attcatcgtg aatggcgcgc gcgagattga
     1381 ccgaatgggg atggtagtag taggcggagg cgccgttgtt gacgagcggg ttgacgccgt
     1441 cgggaagggc gttgttgtgg atcgacacga agaggtcggc ctcggcggcg gcggcgattt
     1501 tcggccggtc ggccaactcg acgtgggcgg tgtcgatccg ggtcattacg acgcgcgcgc
     1561 cggccccctc gagacggtcg cgcagctcga gggcgatcgc caggttcgcc tcggcctcgc
     1621 tcagaccggt cgggccgacc gagccgggat cacgggaatg gccgggatcg aggacgatga
     1681 ctttcccctt caggtcgtcg acatgcgcgg gggcgcggta aacctgcagg tagagcgtgc
     1741 tcccccggta gtacgcatcg tacccccaga gatccttggt caggcggatc gtcagttcgt
     1801 agagcccctc ctccggctgc gaccaggcgg cgtagtcgat gagcgggtcg cgggggtcgt
     1861 ag
//
LOCUS       DAOOKS010000437         1858 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_79579_length_1858_cov_3.127011, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000437 DAOOKS010000000
VERSION     DAOOKS010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79579_length_1858_cov_3.127011"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1423)
                     /locus_tag="PK112_07235"
     CDS             complement(<1..1423)
                     /locus_tag="PK112_07235"
                     /inference="COORDINATES: protein motif:HMM:NF025229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HPC11877.1"
                     /translation="MRARLITALIFGMVLGGGPASEAATFRVPADYHTIQGAIDAADG
                     EDTVLVSDGVWTGPGNYDLVVPGTTIVIASVHGPAVTIIDCAGADFLDHRAFDLGGGG
                     LGAGRDNTFALRGFTIRNGVAAGGGGALVGDRWRLDIEYCIFESNRAVDGGAVSCDSC
                     RVDIRTSRFRYNLAERGGGALYGVGGGVISGCRFDLNRAETGGALADFRDLTFLDCVF
                     QQNEARYGAALRPLPADSVSFAASGTTFSRNRGDAAVFIAYGAADLSHCTFAENDGGL
                     ALTAAAPILTDCILADNAGKPVVCFDPMPGRTSDPFLSYCCVSDSGLDLPGCAVSFFT
                     MPNNLIADPMFCYLTPDDYRLEAGSPCVGAASDGGNMGAAPTGCSATDVGSDPDPPLA
                     AAFRLDQNWPNPFNPSTAIGYTLPQSSHVRLVVYDVLGRVVRVLVDAHRPAGAHTAEW
                     DGLTSSGAPAASGVYVYRFTAGPL"
     gene            complement(1558..>1858)
                     /locus_tag="PK112_07240"
     CDS             complement(1558..>1858)
                     /locus_tag="PK112_07240"
                     /inference="COORDINATES: protein motif:HMM:NF018534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11878.1"
                     /translation="PDHNPQCTGLIQTDHPVIASPESDRVYELRPHVPRQYQKIPFRA
                     SIAHTGRRIHWFLDHELFATSAPGDDVFYDPEPGPHTLMVVDDFGRSSTVSFVVQ"
BASE COUNT          331 a          563 c          693 g          271 t
ORIGIN      
        1 acagtggccc ggcggtgaac cggtagacgt agacaccgct ggcggccggt gcgccggagg
       61 acgtcagacc gtcccactcc gccgtgtggg cgccggccgg ccggtgggca tcgacgagca
      121 cccgaaccac ccgcccgagg acgtcataaa cgacgagccg cacgtgcgag gactggggaa
      181 gggtgtagcc gatggcggtc gacggattga acggattcgg ccagttctga tcgaggcgga
      241 atgcggcggc gagaggggga tcgggatcgc tgcccacgtc ggtggcggag cagccggtcg
      301 gggccgcgcc catgttgccg ccgtcggagg cggccccgac gcacggggat cccgcctcga
      361 gccggtagtc atcgggagtg aggtagcaga acatcgggtc ggcgatgagg ttgttcggca
      421 tggtgaagaa ggacaccgcg cacccgggga gatccaggcc cgaatccgaa acgcagcagt
      481 aactgaggaa ggggtcgctc gtgcggccgg gcatcgggtc gaaacaaaca accggtttcc
      541 cggcgttgtc ggccaggatg cagtcggtca ggatcggagc ggcggcggtg agcgccaggc
      601 ccccgtcgtt ctcggcgaaa gtgcagtggg agaggtcggc cgcgccgtag gcgatgaaaa
      661 cagcggcgtc gccgcggttg cggctgaagg tggttcccga ggccgcgaac gagacgctgt
      721 cggccggcag gggccgcagg gcggcgccgt agcgggcctc attctgctgg aacacacagt
      781 cgaggaaagt aaggtcgcgg aaatcggcga gcgcgccgcc ggtctcggcg cggttgagat
      841 caaaccgaca gccgctgatg acgcccccgc ccacgccgta cagcgcgccc ccgccgcgct
      901 cggcgaggtt gtagcggaag cgcgaggtgc ggatatcgac ccggcaggag tcgcaagaga
      961 cggcgccgcc gtcgacggcg cggttgcttt cgaaaatgca gtattcgatg tcgaggcgcc
     1021 agcggtcgcc gacgagcgcg ccgccgccgc cggccgcgac cccgttgcgg atcgtgaacc
     1081 cgcgcagcgc gaacgtgttg tcgcgccccg cgccgagccc gccgccgccg agatcgaacg
     1141 cgcggtggtc gagaaagtcg gctcccgcgc aatcgatgat cgtgaccgcc gggccgtgga
     1201 ccgatgcgat gacaatcgtg gtgccgggga cgaccagatc gtaattgccg ggaccggtcc
     1261 agacgccgtc ggataccagc accgtatcct cgccgtcggc ggcgtcgata gcgccctgga
     1321 tggtgtggta gtcggcgggg acgcggaaag tggcggcctc ggatgccggg ccaccgccca
     1381 gcaccattcc gaaaatcagc gccgttataa gccgggctct catagcgaac cttactgcct
     1441 cacctctcgt cggcggataa ctcatcctgc tgtccgtaat atatgtcgga atcggaacat
     1501 gccaagttct tcgtttgttc gccggccccg gcgcaggatc tgtcaggggc cggcaactta
     1561 ctggacaaca aaggagacgg tgctggagcg gccgaagtcg tcgaccacca tcagggtgtg
     1621 cgggcccggc tcagggtcat aaaagacgtc gtcgcccggc gcgctggtgg cgaacaactc
     1681 gtgatcgagg aaccagtgga tacggcggcc ggtgtgggcg atggaggcgc ggaacgggat
     1741 tttctggtac tgccttggga cgtgcggccg aagttcatac acccggtcgg actccggaga
     1801 agcgatgacc gggtggtcgg tctggatgag gccggtgcac tgcgggttgt gatcgggg
//
LOCUS       DAOOKS010000438         1854 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_79828_length_1854_cov_3.306281, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000438 DAOOKS010000000
VERSION     DAOOKS010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79828_length_1854_cov_3.306281"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="PK112_07245"
     CDS             complement(<1..475)
                     /locus_tag="PK112_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11879.1"
                     /translation="MRLTRFPARPRGPLELFAALNRYALAAYRGERAIYPLVRELERN
                     FSLSRDELEAAQDKALAAIVDHAFAHSPFYRRKFAAVGFKPGDVKGRADLALLPETNK
                     IELVRAADEVLCLPKDRLRAAGTGGTSGIPFTFYRDPRCLLFRRAVDYTTFRYYGW"
     gene            complement(472..>1854)
                     /locus_tag="PK112_07250"
     CDS             complement(472..>1854)
                     /locus_tag="PK112_07250"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPC11880.1"
                     /translation="SGLLVALPFPYHILPFALLGVVVFVIGTLRYPYFGLYLYLIIFF
                     MDPNQIFPFMQTVAFPYEKVLAFAVIGILLIHIAILKKEFDLYRLDVGFAAMILATYL
                     SILGAVDLAAAWEDWQRFLRLFLVYLVMVRIVKTEGQLKAIIMLFIISIGFLAISSTI
                     QYYRGEFEVRQGIQRLHSFGGGLIDPNTMASSLVLGVPFMFYMAKAHRSALLKLFMFA
                     LIGSCFWAIMLTGSRGGMVGGIALMLLLVWHSRHKTLGFVVAFAVLVCIAAVMPDQYQ
                     NRFMSIFRINEQDEFGASDSARGRINGFKLGFKFLLKNPLTGVGISNFGWHHHLEPNG
                     DWTDAHNLVGKLVGELGLIGVAAFSFFIARFAGTLKLIKRLYDEHGWPPDFLFQMRQA
                     IKIGLIMLFVQGLFGHNLFRDNWYFTAGFIVIVSKIVWDRHLAAQRPSGATPGSPEPL
                     PGAEKGLLPG"
BASE COUNT          394 a          453 c          722 g          285 t
ORIGIN      
        1 gccagccgta gtagcggaag gtggtgtaat cgacggcgcg gcggaagagg agacagcgcg
       61 ggtcgcggta gaaggtgaag gggatgccgg aggtcccgcc ggtgccagcg gcgcggaggc
      121 ggtctttcgg caaacagagg acctcgtctg cggcgcggac gagctcgatc ttgttggtct
      181 cggggagcag ggcgaggtcg gcccggccct tgacgtcgcc cggtttgaac ccgacggccg
      241 cgaatttgcg ccggtagaac gggctgtggg cgaaggcgtg gtcgacgatc gcggcgagcg
      301 ccttgtcctg ggcggcctcg agctcatcgc gggagaggga gaagttgcgc tcgagctcgc
      361 ggacaagggg gtagatggcg cgctcgcccc gataggcggc gagggcgtag cggttgaggg
      421 cggcgaacag ttcaaggggg ccgcgcgggc gggcgggaaa gcgcgtaagt ctcatccggg
      481 caggagtcct ttctccgcac cggggagggg ttcgggggag ccgggggtgg cgcctgacgg
      541 gcgctgggcg gcgaggtggc ggtcccagac gattttgctg acgatgacga tgaagccggc
      601 cgtgaagtac cagttgtcgc ggaaaagatt gtggccaaac aggccctgca cgaagagcat
      661 aatgaggccg atcttgatgg cctggcgcat ctggaaaagg aagtcgggcg gccagccgtg
      721 ttcgtcgtac aggcgtttga tgagtttgag cgtgccggcg aagcgggcga tgaagaagct
      781 gaaggcggcg acgccgatga ggccgagttc gccgacgagt ttgccgacca ggttgtgggc
      841 gtcggtccag tcgccgttcg gctcgaggtg gtgatgccag ccgaaattgg agatgccgac
      901 gccggtcagg ggattcttca ggaggaattt gaagccgagt ttgaagccgt tgatgcgccc
      961 gcgggccgaa tcggaagcgc cgaattcatc ctgctcgttg atgcggaaaa tggacatgaa
     1021 gcggttctga tactgatcgg gcatgacggc ggcgatgcag accagaacgg cgaaggcgac
     1081 gacgaagccg agcgttttgt ggcgcgagtg ccagacgagg aggagcatca gggcgatgcc
     1141 accgaccatg ccgccgcgcg atccggtgag catgatggcc cagaaacagg agccgatgag
     1201 ggcgaacatg aacagcttca acagcgcgct gcggtgggcc ttggccatgt agaacatgaa
     1261 gggaacgccg aggacgaggc tcgaggccat ggtgttgggg tcgatgagcc cgccgccgaa
     1321 agagtggaga cgctggatac cctggcgcac ctcaaactcg ccgcggtagt actggatggt
     1381 ggacgagatg gccaaaaacc cgatggagat gatgaagagc atgatgatgg ctttgagctg
     1441 cccctccgtc ttgactatcc tgaccatgac caggtagacc agaaacaggc ggaggaagcg
     1501 ctgccagtcc tcccaggcgg cggcgagatc gacggcgccg aggatggaga ggtaggtggc
     1561 gaggatcatc gccgcaaaac cgacgtcgag ccggtacagg tcgaattcct tcttcagaat
     1621 agcgatatgg atgaggagga tgccgatgac ggcgaaggcg agaactttct cgtaaggaaa
     1681 cgcgaccgtc tgcatgaagg ggaagatctg gttggggtcc atgaagaaaa tgatgaggta
     1741 gaggtagagg ccgaagtagg ggtagcgcag ggtgccgatg acgaacacga cgacgccgag
     1801 gagcgcaaac gggaggatgt ggtaggggaa ggggagggcg accagcaggc ccga
//
LOCUS       DAOOKS010000439         1852 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_79967_length_1852_cov_3.074569, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000439 DAOOKS010000000
VERSION     DAOOKS010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79967_length_1852_cov_3.074569"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="PK112_07255"
     CDS             complement(<1..298)
                     /locus_tag="PK112_07255"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPC11881.1"
                     /translation="MSDNGVILELRRVVRRVEAKTIIDDLSAQFRKGRIYSVIGPSGA
                     GKSSLLRLVNRLDEIGSGEIRFHGLDIRELPPTRLRCRIGYLFQTPYLFPGANDY"
     gene            complement(295..1245)
                     /locus_tag="PK112_07260"
     CDS             complement(295..1245)
                     /locus_tag="PK112_07260"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HPC11882.1"
                     /translation="MEAKRISEFAVDDTITAFFSVRKREVREFTRGKFLSLELGDASG
                     RIPAVVWEPDQFALEELAEGMVVKAAGKVTEYNGRLQLTITRIRLAAEDEYRLEDILP
                     HSKQPLEQRRARILTLTRQIDNSHIRRLVESFWEDQAFFARFLAAAAGKLWHHACVGG
                     LSEHSANVGELALRVAAGYDFLNRDYLIFGGLLHDLGKVNTYAGAAAIDYTDEGRLVG
                     HICIADVWVCERAARIEGFPPALLTKLRHLLLSHQGEFETPVRPMMPEAFVLYYCDEI
                     DSKLGAMSRIRERQGKPGWSEYVKLIDRFLYFDDTAQEPR"
     gene            complement(1303..>1852)
                     /locus_tag="PK112_07265"
     CDS             complement(1303..>1852)
                     /locus_tag="PK112_07265"
                     /inference="COORDINATES: protein motif:HMM:NF016246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HPC11883.1"
                     /translation="PTMREIFTIGHSRLPFATFLGLLSPHGIAAIVDVRTRPYSRFAP
                     QFNKSRLEAGLKAAGLDYLYFGDRLGGLPEDDRFYDDEGYVRYDRLAASPAFQAGIAD
                     LLEAAGRTRLVLMCAEEDPDRCHRRLLIAPELAARGVAVRHLRADGRAQADESGGAPL
                     LLFPVSPAPVRRSPAPVRPRRV"
BASE COUNT          314 a          586 c          658 g          294 t
ORIGIN      
        1 tgtaatcgtt cgcacccgga aagagatacg gcgtctggaa gaggtagccg atgcggcagc
       61 gaaggcgggt ggggggcaac tcccggatgt cgaggccgtg gaagcgtatt tcgccgctgc
      121 cgatttcgtc gaggcggttg accagccgca ggagcgagga ttttcccgcc cccgaggggc
      181 cgatgaccga gtagatccgc cctttccgga actgcgccga gaggtcatcg atgatcgtct
      241 ttgcctcaac gcggcgaacg acgcggcgca gttccagaat gacgccgtta tcgctcaccg
      301 gggctcctgg gcggtatcgt cgaaatacag gaatcggtcg atcagtttca catactccga
      361 ccagcccggt ttcccctgcc gttcgcggat gcggctcatc gcgccgagtt tggagtcgat
      421 ttcatcgcag tagtagagca caaacgcctc cggcatcatc ggcctcaccg gcgtctcgaa
      481 ttctccctgg tgcgacagga ggaggtggcg gagcttggtc agcagggccg gggggaatcc
      541 ctcgatgcgg gcagcccgct cgcacaccca gacgtcagcg atgcagatgt gcccgaccag
      601 gcgtccctca tcggtgtagt cgattgcggc cgccccggcg taagtgttca ccttgccgag
      661 atcgtggagc aggccgccga agatgaggta gtcgcggttg aggaaatcgt accccgcggc
      721 cacgcggagg gcgagttcgc cgacgttggc ggagtgttcc gagagcccgc ccacgcaggc
      781 gtggtgccag agtttgccgg cggccgcggc gaggaaccgg gcgaagaacg cctggtcctc
      841 ccagaacgac tcgaccagcc ggcggatgtg gctgttgtcg atctgccggg tcagggtgag
      901 gatccgggcc cggcgctgct cgagcggctg tttcgaatgc gggaggatat cctcgagccg
      961 gtactcgtcc tccgccgcca ggcggatgcg ggtgatagtg agctggaggc ggccgttgta
     1021 ctcggtgact ttcccggccg ccttgaccac catcccctcc gcgagttcct ccagagcgaa
     1081 ctggtcgggt tcccagacga cggcggggat ccggccgctg gcgtcgccga gttcgaggga
     1141 gaggaatttc ccgcgggtga actcgcgcac ctcgcgcttg cgtacggaga agaaggcggt
     1201 gatcgtgtcg tcaaccgcga attcgctgat ccgcttggcc tccatggcgc caagatacgc
     1261 gcgcggcgcg cgccgcgcaa gcggcggcga ccggccggcc gttcaaaccc gccgcgggcg
     1321 caccggcgcc ggcgagcggc gcacgggagc gggtgagacc ggaaagagca acaacggcgc
     1381 gcccccgctt tcgtccgcct gcgcgcgtcc gtccgcccga agatggcgca ccgcgacccc
     1441 ccgggcagcc agctccgggg cgatcaggag ccgccgatgg caccggtcgg ggtcttcctc
     1501 cgcacacata agcaccaatc gcgttcggcc ggcggcttca aggagatcgg cgatgccggc
     1561 ctggaacgca ggcgacgccg ccagccggtc gtaccgcaca tacccttcgt cgtcgtagaa
     1621 ccgatcgtcc tcgggcaggc cgccgaggcg gtccccgaaa tagaggtagt caaggccggc
     1681 ggctttcagg ccggcctcca ggcgcgactt gttgaactgc ggcgcaaatc gggagtaggg
     1741 gcgggtgcgg acgtcgacga tggcggcgat gccatggggg gagagcaggc ccagaaacgt
     1801 cgcgaacggg aggcgcgagt gcccgatggt gaagatctcc ctcatggttg gg
//
LOCUS       DAOOKS010000440         1851 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_80030_length_1851_cov_2.989978, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000440 DAOOKS010000000
VERSION     DAOOKS010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80030_length_1851_cov_2.989978"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            140..790
                     /locus_tag="PK112_07270"
     CDS             140..790
                     /locus_tag="PK112_07270"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HPC11884.1"
                     /translation="MTTHMRKTASLPIDDVAGMLANLGFRTMCGVDEAGRGPLAGPVV
                     AAAVIVPHGFALDGLTDSKLMTPAQRDEVFARIAEAGFPCAVGIIDHETIDRVNILKA
                     SLLAMRRAVMELPAPPECVIVDGNHTIPNLAFPQYALVHGDRLCPAVSAASVIAKVTR
                     DRIMDRMERLYPQFRFSQHKGYCTPAHLEELRAHGPCEIHRKSFGPVAELLQYALV"
     gene            777..1160
                     /locus_tag="PK112_07275"
     CDS             777..1160
                     /locus_tag="PK112_07275"
                     /inference="COORDINATES: protein motif:HMM:NF014119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HPC11885.1"
                     /translation="MPSSKRPRGKGTGRVAAGRRYERLAADFFRANGFEVRAQNFRAG
                     AREIDLIVQRDRLVVFVEVKAARDESFGHPAERVDGRKREHLVAAARRYMGENDLSGC
                     DLRFDCITFVGGQIEHYPNAFLVEE"
     gene            complement(1171..1563)
                     /locus_tag="PK112_07280"
     CDS             complement(1171..1563)
                     /locus_tag="PK112_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11886.1"
                     /translation="MVWFRRLLALALVLLAVGGYAYVRQHRRAAAQAERAHLALLTAR
                     IWVGTAEYRDRPAVFLAWRDSLLAAENLTADSLRVRVDRLRADPSASGDYFRQVSRFV
                     DSLVRIRTAPAETSAGSAPPPGVRVRRE"
     gene            1644..>1851
                     /locus_tag="PK112_07285"
     CDS             1644..>1851
                     /locus_tag="PK112_07285"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HPC11887.1"
                     /translation="MELTYPIRRRKSKAVKIGSVIIGGGFPVAIQSMTTADTRNVEAT
                     VAQTLDLFAAGCDIVRLSVLNHEAA"
BASE COUNT          315 a          649 c          610 g          277 t
ORIGIN      
        1 cccctcgctt ccccgcccgc aatatacccc ccggcggccg ctaaacacac ggcttttccg
       61 cgccgataga atgggggaga gccggatttt tgttgccggg acggtcccgg cgcggcctag
      121 ttgcgatatg gcggacctca tgacgaccca catgcgaaaa acggcttccc tgccgatcga
      181 cgacgtcgcg gggatgctgg cgaacctcgg attccgcacc atgtgcggcg tcgacgaggc
      241 cgggcgggga ccgctcgccg gaccggtggt ggcggccgcc gtcattgtgc cgcacgggtt
      301 cgctctcgac ggactcactg attccaagct catgacgccc gcgcagcgcg acgaggtgtt
      361 cgcccggatc gccgaagcgg gctttccctg cgcggtcggc atcatcgacc acgagaccat
      421 cgaccgggtc aacattctga aagcctcgct tctggcgatg cgccgggcgg tgatggaact
      481 gcccgcccct cccgagtgcg tgattgtcga cggcaaccac acgattccta acctggcctt
      541 cccccagtac gcgctcgtcc acggcgaccg gctctgcccg gcggtctcgg cggcctcggt
      601 gatcgcgaaa gtgacccgcg accggatcat ggatcgcatg gaacgcctct acccgcagtt
      661 ccggttctcc cagcacaagg ggtactgcac gcccgcccac ctcgaggaac tccgcgccca
      721 cgggccgtgc gagattcacc gcaagtcttt cggccccgtc gccgaactgc tccagtatgc
      781 cctcgtctaa gcgcccccgc ggcaagggga ccggccgggt ggccgccggc cgccgctacg
      841 aacggctggc cgccgatttc ttccgcgcca acggtttcga agtgagagcg caaaacttcc
      901 gcgccggagc gcgcgagatc gatctcatcg tgcaacgcga ccgcctcgtc gtgttcgtcg
      961 aggtgaaagc cgcgcgcgac gagagcttcg ggcacccggc cgaacgggtc gacggacgca
     1021 agcgggaaca cctcgtggcc gccgcccggc gctacatggg cgaaaacgac ctgagcggct
     1081 gcgatctgcg cttcgactgc attacctttg tcgggggaca aatcgagcac tatcccaacg
     1141 cgttcctggt cgaggagtag gcgccggccg ctactcgcgg cgcacccgca ccccgggggg
     1201 cggcgccgaa ccggccgagg tttccgcggg cgccgtccgg atccgcacca gcgaatccac
     1261 gaaccggctg acctggcgga agtagtcccc ggaggccgac ggatcggcgc gcaggcggtc
     1321 gacccggacg cggagcgagt cggcggtgag gttctcggcg gccagcagag agtcgcgcca
     1381 ggccaggaag acggccgggc ggtcgcggta ctcggctgtg ccgacccaga tccgggcggt
     1441 cagcagggcg aggtgtgcgc gctccgcctg tgcggccgcg cggcggtgct gccggacgta
     1501 tgcgtacccg ccgactgcaa ggaggacgag cgcgagggcc aaaagccgcc tgaaccagac
     1561 cacgatgcca ccaactttcg gttgacgcga cgcgtaaaac tctgtaaact accgggcttt
     1621 tcacccgagg tcaaggctga acaatggaac tgacttaccc catccggcgc cgcaagagca
     1681 aggcggtcaa gatcggcagc gtgatcatcg ggggcgggtt ccccgtcgcg atccagtcga
     1741 tgaccacggc cgatacacgc aacgtcgagg ccacggtcgc gcagacgctt gacctgttcg
     1801 cggccggctg cgacatcgtc cgcctctccg tgctcaacca tgaggccgcc g
//
LOCUS       DAOOKS010000441         1851 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_80041_length_1851_cov_2.752784, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000441 DAOOKS010000000
VERSION     DAOOKS010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80041_length_1851_cov_2.752784"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..1331)
                     /locus_tag="PK112_07290"
     CDS             complement(135..1331)
                     /locus_tag="PK112_07290"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF006058.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HPC11888.1"
                     /translation="MSLGVLNRFRRQEPRPDRALRRAVEACDPLAFHRTLPGYCPTPL
                     VSLPGQARRLGLREVLVKDEAHRLGLNAFKVLGASYAVYRFLRRQWEDRQSAPFGDTL
                     ALTPEQRRFFEGATLCTATDGNHGRAVAWSARVVGLPAVIYLPAGSATARVENIRREG
                     ARAEVINGGYDDAVRQIRNDAAARGWQIISDTAWPGYTEIPAWIQAAYTTLFRELEQT
                     LARPGEPGVDAVIVQGGVGALAAGAAWHYAEQYGARRPRLICVEPVGAACLLASARSP
                     KGTPVPAGNPAPTIMAGLNCREVSVAAWPLIRDTFDAFVAIEDDYAVEAMNRYYRPEP
                     GDPRIIAGESGAAGLAALLALCADPALADLRSALGLGPASRVLLLNTEGATDPVGFAR
                     AVGAQP"
     gene            1430..>1851
                     /locus_tag="PK112_07295"
     CDS             1430..>1851
                     /locus_tag="PK112_07295"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HPC11889.1"
                     /translation="MKILTRYILAEHIGPFLFAFFTITFLLVIEYVPKIIDHVIDKDL
                     SIWVALELVGLNLAWMLALSVPMSVLVAVLMAFGRLTADFEVTAMKASGINLLRATIP
                     LLIAGGVIAYGMVEFNDKVLPDVNKKARHLWGDISAMRP"
BASE COUNT          289 a          595 c          677 g          290 t
ORIGIN      
        1 cttgttgaac tgcggcgcaa atcgggagta ggggcgggtg cggacgtcga cgattgcggc
       61 gatgccctgg ggggagagca tgcccggaaa cgtcgcggac gggagccgcg cgtgcccgct
      121 ggtggagatc tccctcatgg ttgggcgccc acggcgcgcg cgaacccgac cgggtcggtc
      181 gcgccctcgg tattgagcag cagcacccgc gaggcggggc cgaggccgag cgcagagcgg
      241 agatcggcca gggcggggtc ggcgcacagg gcgaggagcg cggccaggcc cgcggcgccg
      301 gactcgccgg cgatgatcct cgggtcgccc ggctcgggac ggtagtaccg gttcatcgcc
      361 tccaccgcgt agtcgtcctc gatggcgacg aaggcatcga aggtgtcgcg gatgagcggc
      421 caggccgcta ccgaaacttc ccggcagttg agtccggcca tgatcgtggg cgcggggttg
      481 ccggccggca cgggagtccc cttcggcgag cgggccgagg cgagcaggca ggcggcgccg
      541 accggttcga cacagatgag tcggggccgc cgggcgccgt actgctccgc gtagtgccag
      601 gcggctccgg cggcgagggc gccgacgccc ccctgcacga tcacggcgtc cacgcccggc
      661 tcacccggcc gggcgagggt ctgctcgagc tcgcgaaaaa gggtcgtgta ggcggcctga
      721 atccaggccg ggatttcggt ataccccggc caggcggtat cggaaataat ctgccagccg
      781 cgggcggcgg cgtcgttccg gatctgccgg accgcgtcat cgtacccgcc gttgatcacc
      841 tccgcccgcg ccccctcgcg gcggatgttt tccacccggg cggtcgcgct gccggcgggg
      901 aggtagataa ccgccgggag cccgacgacc cgggctgacc aggccaccgc gcggccgtgg
      961 ttgccatcgg tcgccgtgca cagcgtcgcc ccctcgaaga atcgccgctg ctccggcgtg
     1021 agggccagcg tatcgccgaa gggcgcgctc tgccggtcct cccactgccg gcggaggaag
     1081 cggtagacgg cgtaggatgc gccgaggact ttgaaagcgt tcagccccag gcgatgggcc
     1141 tcatccttga ccagcacttc gcgcaggccg aggcggcgcg cctggccggg cagggacacg
     1201 aggggtgtcg gacagtaacc gggcagggtg cggtggaacg cgagcggatc gcaggcttcg
     1261 accgccctcc gcagcgcccg gtcggggcgc ggttcctggc gccgaaagcg gttgagaact
     1321 ccgagactca cgggaggcat agtacgcaac cgacgggggg aagtaaagcc gtcgcgggcg
     1381 aattcatact tggcggtcca ttgtataacc aatatatttg cttgcactta tgaagatact
     1441 gacgcgctac atactggccg agcacatcgg gccgttcctg ttcgcctttt tcaccatcac
     1501 cttcctgctg gtgatcgagt acgtgccgaa aatcatcgac cacgtcatcg acaaggatct
     1561 ttcgatctgg gtggccctgg agctggtcgg gctcaacctg gcgtggatgc tcgcgctttc
     1621 ggtgccgatg tcggtgctgg tggcggtgct gatggcgttc gggcggctca ccgcggattt
     1681 tgaggtgacg gccatgaagg cctccggcat caacctcctg cgcgcgacca tcccgcttct
     1741 gatcgccggc ggggtgatcg cctacgggat ggtcgagttc aacgacaagg ttctgcccga
     1801 tgtgaacaag aaggcgcgcc acctctgggg ggacatctcg gccatgcggc c
//
LOCUS       DAOOKS010000442         1849 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_80182_length_1849_cov_1.653846, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000442 DAOOKS010000000
VERSION     DAOOKS010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80182_length_1849_cov_1.653846"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1640
                     /locus_tag="PK112_07300"
     CDS             <1..1640
                     /locus_tag="PK112_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11890.1"
                     /translation="GQVAVKAWEALGKATGIAYDFGLPDDLNAMPEGYGKLIDRVIAS
                     MKYTGQNLFAYPGAWYQGLMNDDYMPRRHPPDFLSAWYEKFDRIGLGVVPTVNPNNMP
                     VKPGLVTRQAMSDGSLHPTPIAIHDTGKPNWGKWHDTPPNFNFGHPEVRKHIESIVDA
                     LLAQGREHPSFKGVCMHMTRHGLLWFGDEESGYNDYCIEEFVERLGGWNVEKLKGLNK
                     VDRRDPLRGKAYAASLRADPVLWEKWLKWRCDRVTEFYAGIAAKLRTVRPDLKLWLNS
                     FVPANYNHPDFLRPDFMHRANMACGLDGPALTKAAPNLILCQTMVPADYRHNWRGAYK
                     LPGAYEHQRVLDELPGFYDLLDGADYPWVNQHDRYWESAIGRGAKGAKNTLSCEWMDE
                     CSWRVSTLNPAGRHALKHFVEPLRYHDVLGMSKGGFLIGTYGMEDVLVPFIQAFRALP
                     AVVMKDVDKLGDCEVGAMRARGVVVRECEFDGRTYFYIVNTVYEPRTVTIAFPAGTVD
                     LVTGRSLAADRCGRPVEFVLKPYEFRSFSSPATLHGR"
     gene            1648..>1849
                     /locus_tag="PK112_07305"
     CDS             1648..>1849
                     /locus_tag="PK112_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11891.1"
                     /translation="MTTGMYDIYTGGIFDMWARMPTRPVRAGRWRVRLRRKRSGEVPE
                     KGQNPKQTEKTAAGLILREGNRK"
BASE COUNT          367 a          538 c          590 g          354 t
ORIGIN      
        1 acggccaggt cgcggtgaaa gcctgggagg cgctcggcaa ggcgaccggc atcgcctacg
       61 atttcggtct cccggacgat ctcaacgcca tgcccgaggg ttacggaaag ctcattgacc
      121 gtgtcatcgc ctcaatgaag tatacggggc agaatctttt tgcctatccg ggcgcctggt
      181 accagggtct catgaacgac gactatatgc cgcggcgaca tccgccggat ttcctgagtg
      241 cctggtacga gaagtttgac cggatcggtc tcggggtcgt gccgacggtg aaccccaaca
      301 acatgccggt gaagccgggg ctcgtgacgc ggcaggcgat gtccgacggc tcgctccacc
      361 cgacgccgat cgcgatccat gacaccggca agcccaattg gggcaagtgg cacgatacgc
      421 cgccgaactt caacttcggt catcccgaag tcaggaagca catcgagtcg atcgtcgacg
      481 cgcttctcgc ccaaggcagg gagcatccgt ccttcaaggg cgtttgcatg cacatgaccc
      541 gccacggcct cctgtggttc ggcgatgagg agtcggggta taacgactac tgcatcgaag
      601 aatttgttga gaggttggga ggttggaacg ttgagaagct gaaaggtttg aataaggtgg
      661 atcggaggga tccgcttcgc ggcaaggcct acgccgcgtc gctgagggcc gatcctgtcc
      721 tgtgggagaa gtggctcaag tggcggtgcg accgggtgac ggagttttac gcgggcatcg
      781 cggcgaaact gcgcacggtg cgtcccgacc tcaagctctg gctcaacagc ttcgtgccgg
      841 cgaactacaa ccatcccgat tttctccgcc ccgatttcat gcaccgggcg aatatggcgt
      901 gcggactgga cggtcccgcg ctcacaaagg cggcaccgaa tctcatcctc tgccagacga
      961 tggtgcccgc cgactaccgc cacaactggc gcggtgccta caagctcccc ggcgcgtacg
     1021 aacaccagcg tgtcctcgac gaactgcccg gtttctacga tctgctcgac ggggcggatt
     1081 atccgtgggt caaccagcac gaccgctact gggaatcggc gatcggccgt ggcgcgaagg
     1141 gggcgaagaa cacgctctcg tgcgaatgga tggacgaatg ttcttggcgg gtttcgacgc
     1201 tcaatcccgc cggacgccac gcgctgaaac attttgtcga accgctccgt taccacgacg
     1261 tgctcggcat gtccaagggc gggttcctca tcggcaccta cggcatggaa gacgttctcg
     1321 tgccgttcat tcaggccttc cgtgcacttc cggcggtagt catgaaggac gtcgataagt
     1381 tgggagactg cgaggtcggg gcaatgagag cgcggggcgt cgtcgtcagg gaatgcgaat
     1441 tcgacggtcg tacctatttc tacatcgtca acacggtgta cgagccgcgc acggtgacga
     1501 tcgcgtttcc tgcgggtacg gttgatctcg tcacgggacg ttcgctggcg gctgatcggt
     1561 gcgggcgacc ggttgaattc gtgcttaagc cctatgagtt tcgtagcttc tcgtcaccag
     1621 cgacccttca tgggaggtag gtccctggtg acgaccggca tgtacgatat ctataccggc
     1681 ggcatcttcg acatgtgggc gcggatgcca acgcgcccgg tgcgcgctgg tcgttggcgc
     1741 gtacgattac ggcggaaacg gtctggtgaa gtaccagaga aaggccaaaa cccgaagcag
     1801 accgagaaaa cggccgcagg cttgatcctg cgtgaaggaa accgcaaaa
//
LOCUS       DAOOKS010000443         1848 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_80224_length_1848_cov_3.031790, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000443 DAOOKS010000000
VERSION     DAOOKS010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80224_length_1848_cov_3.031790"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1234
                     /locus_tag="PK112_07310"
     CDS             <1..1234
                     /locus_tag="PK112_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11892.1"
                     /translation="IGDILDFSLATYEEVYEAAEVFFGALAGTAEYFVYVPGNHDFDV
                     WTTVEYQVNIINRLDRGKPVRPFRWTVPAVFDDRPGRRDRQVLPTVDPSPEGPPYGGL
                     FLDALGTGGGGRSRFIFAYPNAYLATADGELIMMTHGHYFEWYWSALYEAVTGIVAGH
                     PPGAGEAEPADPTLEQMVAVNSTACQLSCSGIGQAGLLSRVINEVVAEVKVESRHRSL
                     AATSRYLDNAIAYVDAYTRIGGDLFDRLDLDERVMEAIKARLMRKLRAYEAARHRVPF
                     LRNKGTRERFGAYFAAGAREIAALNERHACALPESPGAVIFGHTHRPLRWEPTRVPNA
                     DYDYPYRGRTVRLFNAGAWINREREGRGQVFSGAEVFLYDSDIPGGMTSVAIEAEAGP
                     GPEMIADGPGASDGPEGS"
     gene            1231..>1848
                     /locus_tag="PK112_07315"
     CDS             1231..>1848
                     /locus_tag="PK112_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11893.1"
                     /translation="MSANRVPDGDGVIDAHVHVGLRDGWNGWGRMSAAYASEANLTFR
                     IFALYAGLAGEAITDERLVAAAIDYALSAPVRSVVMLALDHVYERDGRPKPERSHLWV
                     HNDYILYLRAEVARRGQPGKVLFGASVHPYRTDFAERVRECADKGAVLLKWVPSAQDI
                     DLASETVAERLEFLAAFRKPNGKPLPLLLHVGPEYAIPPADPAGTS"
BASE COUNT          281 a          565 c          715 g          287 t
ORIGIN      
        1 gatcggcgac atcctggatt tttcgctcgc gacctacgaa gaagtctacg aggcggcgga
       61 ggtgtttttc ggcgccctgg ccgggacggc ggagtacttc gtctacgtgc cgggcaacca
      121 cgacttcgac gtctggacga cggtcgagta ccaggtgaac atcatcaatc ggctcgaccg
      181 cgggaagccg gtgcggccgt ttcggtggac ggtgccggcg gtgttcgacg accggcccgg
      241 gcggcgcgac cgccaggtgt tgccgacggt ggacccgagt ccggagggtc cgccctacgg
      301 cgggctgttc ctcgacgcgc tgggaacggg cggcggcgga cgaagccggt tcattttcgc
      361 ctaccccaat gcctacctgg cgaccgcgga cggggaactc atcatgatga cccacgggca
      421 ctatttcgag tggtactgga gcgccctcta tgaggcggtc acggggattg tcgccgggca
      481 tccgccgggc gccggggagg cggaaccggc ggacccgacg ctggagcaga tggtggcggt
      541 gaactcgacc gcctgccagt tgtcgtgctc ggggatcggg caggcggggc tgctgagccg
      601 ggtgatcaat gaagtggtgg cggaggtcaa agtggagagc cgccaccggt cgctggcggc
      661 gactagccgc tacctggaca acgccatcgc ctatgtcgat gcgtatacgc ggatcggcgg
      721 cgacctgttc gaccggctcg atctcgacga gcgggtgatg gaggcgatca aggcgcgact
      781 gatgcggaag ctgcgggcgt acgaagcggc gcgccaccgc gtcccctttc tccgcaacaa
      841 ggggacgcgg gagcggttcg gggcgtattt cgcggccggc gccagggaga ttgcggccct
      901 caacgagcgg cacgcgtgcg cgctgccgga gtcgccgggc gccgtgattt tcggccacac
      961 ccaccggccc ctgcggtggg agccgacgcg cgtgccgaac gcggactatg actaccccta
     1021 tcggggccgg acggtccggc tgttcaacgc cggagcgtgg atcaaccggg agcgggaggg
     1081 ccgcgggcag gtgttctcgg gggcggaggt gttcctgtac gactcggaca ttcccggcgg
     1141 gatgacctcg gtggcgatcg aggcggaggc ggggccgggg ccggagatga ttgcggacgg
     1201 tcccggcgct tcggacgggc cggagggatc gtgagcgcga acagagtgcc ggacggcgac
     1261 ggcgtcatcg acgcccacgt gcacgtcggc ctgcgcgacg gctggaacgg gtgggggcgg
     1321 atgtcggccg cctacgcctc ggaggcgaac ctgacctttc ggatctttgc gctctatgcg
     1381 ggattggcgg gggaggcgat caccgacgag cggctcgtgg cggcggcgat cgactacgcc
     1441 ctgagcgcgc cggtgcgaag cgtagtgatg ctggcgctcg accatgtgta cgagcgcgac
     1501 ggacggccca agccggagcg ttcccacctg tgggtccaca acgactacat cctgtacctg
     1561 cgcgcggagg tggcgcggcg gggacagccg ggaaaagtgc tgttcggggc ctccgtgcat
     1621 ccctaccgca ccgatttcgc cgagcgggtg cgggagtgcg ccgacaaggg agcggtgctc
     1681 ctgaagtggg tgccgtcggc gcaggatatc gacctggcga gcgagacggt ggcggagcgg
     1741 ctggagttcc tcgcggcgtt tcgcaagccc aacggcaagc cgctgccgct tcttttgcac
     1801 gtcgggcccg agtatgcgat ccccccggcc gatcccgcgg ggacgtcg
//
LOCUS       DAOOKS010000444         1843 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_80565_length_1843_cov_2.857942, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000444 DAOOKS010000000
VERSION     DAOOKS010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80565_length_1843_cov_2.857942"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            49..1367
                     /locus_tag="PK112_07320"
                     /pseudo
     CDS             49..1367
                     /locus_tag="PK112_07320"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
     gene            1571..>1843
                     /locus_tag="PK112_07325"
     CDS             1571..>1843
                     /locus_tag="PK112_07325"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HPC11894.1"
                     /translation="MISLVVGLGNIGRRYAGTRHNLGFEVLDRAAAALGAPRATRTGL
                     DERTVCRRGDRTIVLAWPRTYMNRSGDAVAALLQDYGLRPAEVLVVV"
BASE COUNT          275 a          557 c          707 g          304 t
ORIGIN      
        1 cccggcgacg aggcggaggg gatcgtggcg gaggagctcg gggcgccggt ggcggagctg
       61 tttctctcgt tcgagcgggc gccgctggcc gccgcctcga tcggccaggc ccatgcggcg
      121 gtgctgcgca gcggcgagga agtggtggtc aaggtgcgcc gcccgggggt ggtcgagcag
      181 gtgaccgagg atatcgagat tgtccgcaat ctcgcgctgg tggccagccg gagactcggc
      241 ccggccgagc actacgatct gattgggctg gccgaggaat tcgccgagac gctgcgcggc
      301 gaattggact accttcgcga gggacgcaac gccgagcggt tcgccgagca tttccggggg
      361 gatccggaag tgcggatccc gcgggtgtac tgggagcaat cgactgccag cgtgctgacg
      421 ctcgagcgaa ttcggggaat caaggtcgac gacacggcgg cgctcgacgg ggcggggatc
      481 gaccgcaggc aactggcgcg gcggatcgcg cgcgtgctcc tgaaaatgat cctggaggac
      541 ggcttcttca cgccgacccg cacccgggaa atttcttcgt ggaacccgac gggcggctcg
      601 gggtgatgga tttcggcatg gtcggggtga tcgacgaggc ggggcgggag cggctggcga
      661 tgctcctggg ggcgctgacc tcaggggacg ccgagcggct ggttgacacg gtgctcgata
      721 tgagcgccgg ccgggggcgg gtcgaccggg aggaactcaa gcgggacatc cagcgtctgc
      781 tgacccgcta ctatgaactg ccgctggggc agattcccct cgggcggatg ctcgaagagg
      841 cgctggccgc agtccgtcgg caccgcctcc aattgccctc gagcctggcg ctgttgttcc
      901 gggcgctgat cgtgagcgag ggactggggc tgcgcctcga tcccgatttc agcctcggtc
      961 cggtgctcaa accattcgcc gagcggctgc tcctgcaacg ctactcgccg ctgcgctggg
     1021 cgcagcggat ggggcgggcc atgatcgaca ccgaccacct gctctccgaa ctgccgcggc
     1081 ggctccaccg cgcgctcaag atgctcgacc gggggggatt cgagttcggc ctgcggcggg
     1141 agagtctcga accgatggcc gaccggctcg agcggctggc caaccggatc gtgctcggga
     1201 tgatcacggc cgcgtttatc atcgcgctgt cggtgctgat gctcgtctac ccgccggggg
     1261 gcggggaagg gcactggggc gggacggtgt tcgccgtagg gttggtggcg gcagcgggcc
     1321 tgggggtgta cctggcctgg cggattatcc gcaccggccg ggggtaagga cttgccggtc
     1381 aatcctctcc ccccgcggtc cgatcgacgc gcccggagcc gcgcggcgga gaagccttct
     1441 tcccggccgc cgggatgccg gaatgggacc ggccgggccg gaaaaaggtc acacgcgcac
     1501 aatcacataa tctgttgact ttggggcggg atagggtata attgtttgct ttgccggaaa
     1561 cggcagccct atgatttcgc ttgttgtcgg actcgggaat atcggccggc gctatgcggg
     1621 cacccggcac aacctcggat tcgaagtgct cgaccgggct gccgcggcgc tcggagcgcc
     1681 gcgggcgacg agaacgggtc tggacgagcg caccgtgtgc cggcgcggcg accggacaat
     1741 cgtgctggcc tggccgagga catacatgaa ccggtcgggg gacgcggtcg cggctctttt
     1801 acaggattac ggtctccgtc cggccgaggt gctggtggtg gtc
//
LOCUS       DAOOKS010000445         1840 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_80747_length_1840_cov_3.745098, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000445 DAOOKS010000000
VERSION     DAOOKS010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80747_length_1840_cov_3.745098"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1499
                     /locus_tag="PK112_07330"
     CDS             <1..1499
                     /locus_tag="PK112_07330"
                     /inference="COORDINATES: protein motif:HMM:NF015148.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HPC11895.1"
                     /translation="LGVGYIIGRRVSANMMAGGLLSYLILIPAITIFGENLTAPMFPA
                     TTLIRDMSPNEVRNAYVLYIGAGAVATGGIISLIRSFPTIIAAFRRGFRTFLDSRAGK
                     GAQEAVLRTERDLPLSVVVFGSIGLVLAIWLSPILHINVISAVLIVLFGFFFVTVSSR
                     ITGEIGSSSNPISGMTVATLLITCLLFLAVGWTGVSYRAMALSTAAIVCIAASNGGTI
                     SQDLKTGFLVGATPRLQQIAIMIGVISSALVIGLIVIALNNAYTTVTPARHPDYLAAA
                     APGAETQQGPDGAMYRVHYVNEPVGTVLIGKYLVDDSGRIAYLVDPGIAGVVSEVDGK
                     QVTKLDSPKARLFSLIIDGILTQKLPWGLVLIGVFLALMMELVGISSLPFAVGLYLPL
                     SSSAPIMAGGLIRGIVDRKRKSNSAEAEFSPGVLMSSGFIAGAAIMGIVVAGLTGFGL
                     DKAINGSGWSHYLSEADWFSLIPFGALMYMLYRVGVSVKDKGKQKSAA"
     gene            complement(1644..>1840)
                     /locus_tag="PK112_07335"
     CDS             complement(1644..>1840)
                     /locus_tag="PK112_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11896.1"
                     /translation="ALNGFAYFLLTGRFREGLVFVVLSFLAFQIVRPRYGMVRGLIKQ
                     QEDLAARGLLRTQRRPGELT"
BASE COUNT          277 a          659 c          578 g          326 t
ORIGIN      
        1 tcctcggcgt cggctacatc atcggccgcc gggtctccgc gaacatgatg gcgggcggcc
       61 tgctgtcgta cctcattctc atccccgcga tcacgatttt cggcgagaac ctcacggcgc
      121 cgatgttccc ggccacgacc ctcatccgcg acatgtcgcc gaacgaggtg cgcaacgcct
      181 acgtgctcta catcggggcc ggtgcagtcg ccaccggcgg catcatcagc ctcatccggt
      241 ccttccccac catcatcgcc gcgttccgcc gcggcttccg cacgttcctg gactcccgcg
      301 cggggaaggg ggcgcaggag gcggtgctcc gcaccgagcg cgacctcccg ctctcggtcg
      361 tggtctttgg atcaatcggc ctggtcctcg ccatctggct ctcccccatc ctccacatca
      421 acgtcatctc ggccgtcctg atcgtcctct tcgggttctt cttcgtcacc gtctccagcc
      481 gcatcaccgg cgaaatcggc tcctcctcga acccgatctc gggtatgacc gtggccaccc
      541 tcctgatcac ctgcctgctc ttcctggccg tcggatggac gggcgtgtcg taccgggcga
      601 tggctctctc gacggcggcg atcgtctgca tcgccgcctc gaacggcggc acaatcagcc
      661 aggatctcaa aaccggcttc ctcgtcgggg ccacgccccg gctccagcag atcgccatca
      721 tgatcggcgt catctccagc gccctggtca tcgggctcat cgtcatcgcc ctcaacaacg
      781 cctacacgac cgtgaccccc gcccgccacc ccgactacct cgcggccgcc gctcccggcg
      841 ccgagacgca gcagggtccg gacggcgcga tgtaccgcgt gcactatgtc aacgagccgg
      901 tcgggaccgt cctgatcggc aagtacctgg tcgacgattc ggggcggatc gcctacctcg
      961 tcgatcccgg catcgcgggc gtcgtgtcgg aggtcgacgg caagcaggtg acgaagctcg
     1021 attcgcccaa ggcgcggctc ttcagtctca tcatcgacgg catcctcacg cagaaacttc
     1081 cctgggggct ggtgctgatc ggcgtgttct tggcgctcat gatggagctg gtggggatct
     1141 cgtcccttcc cttcgcggtc ggcctctatc tcccgctgtc gagttcggca ccgatcatgg
     1201 caggcggtct catccgcggc attgtcgacc gcaagcgcaa gagcaattcc gcggaggcgg
     1261 agttctcgcc gggcgtgctc atgtcgtccg gattcatcgc cggcgccgcc atcatgggca
     1321 tcgtcgtcgc cggcctcacc ggcttcggac tggacaaggc gatcaacggc tccgggtggt
     1381 cgcactacct ctcggaggcg gactggttct cgctgatccc gttcggggcg ctcatgtaca
     1441 tgctgtaccg ggtgggcgtg tcggtgaagg acaaggggaa acagaaaagc gccgcctgag
     1501 ccggcgcagc ccgacggcaa atgaaaaggc ggcccgcagg cgtatccggc gggccgcgct
     1561 gtttgtcccg ggggggccgc ttatcggcgg ggcgggggag accccggacg ccggaaatcg
     1621 ggcgcggccc tcggccggcc gccctacgtc agctcgccgg ggcggcgctg ggtgcgcagg
     1681 agtccccggg cggccaggtc ctcctgctgt ttgatcaggc cgcgcaccat gccgtacctc
     1741 ggccggacta tctggaacgc gagaaacgag aggaccacga acaccagccc ctcgcggaac
     1801 cgcccggtga gcaggaaata ggcgaacccg ttgagggcga
//
LOCUS       DAOOKS010000446         1840 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_80768_length_1840_cov_2.391597, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000446 DAOOKS010000000
VERSION     DAOOKS010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80768_length_1840_cov_2.391597"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1078
                     /locus_tag="PK112_07340"
     CDS             <1..1078
                     /locus_tag="PK112_07340"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HPC11897.1"
                     /translation="GVAVPRWSPDSRRIFFIGSDTGRTSVYFVSARGGQPTRVFDGKC
                     HVKALSLAAPARTAAIIYSDLATAGEIVTFPAVHGGEKRAVTRTALNPFLVSEVVLGK
                     TKDLMFRSFDGTAVQGWIVYPPAFRPNRKYPSILEIHGGPRMQYGYTFFHEMQWLAAQ
                     GYVVFYTNPRGGQGRGQTWADAISGGWGGLDYQDCMAAADFLAAQPFIDPKRMGVTGG
                     SYGGYMTNWIIGHTDRFKAAVTQRSVVNFMSMYGASDIGWSLERELDGTPWNNSGNYE
                     KCSPITYFTNVRTPVLILHNEQDLRCGIEQAEQMFTMLKRLRRTVEMVRFPEEPHGLS
                     RHGRPDRRIARLTWIARWFDRYLK"
     gene            complement(1324..>1840)
                     /locus_tag="PK112_07345"
     CDS             complement(1324..>1840)
                     /locus_tag="PK112_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11898.1"
                     /translation="VGGNKVLGNKLEVGGQFAVRDNRWECGLDFAARFLGESEPYRVR
                     AGGEIRQTKEFLGLFVGCSTAYEVIRARRWSADVFATVGFDGIRAVSRTGRNEAQWIN
                     SPALGLGFTHRVCFDRGCTRYLGLQWRCTIVDYVTHGGSDLSGNALSLSLVWGAMSSG
                     ADRHLQRLQYF"
BASE COUNT          331 a          646 c          536 g          327 t
ORIGIN      
        1 cggcgtcgct gtcccccgct ggtcgcccga cagccggcgg attttcttca tcggctccga
       61 taccggccgc acgagtgtgt acttcgtctc cgcgcgcggc ggccaaccca cccgcgtgtt
      121 cgacggcaag tgccacgtga aagcgttgtc cctcgccgca ccggcccgca ccgccgccat
      181 catctactcc gatctcgcga cagcgggcga gatcgtgact ttcccggcgg tccacggcgg
      241 ggagaagcgg gcggtgaccc gcaccgctct gaaccccttc ctcgtttccg aggtggtgct
      301 gggaaagacg aaagacctca tgttcaggtc gttcgacggc accgcggtcc aggggtggat
      361 cgtctacccg cccgctttcc ggccgaatcg gaagtatccc tcgattctcg aaatccacgg
      421 cggcccgcgc atgcagtacg gctacacgtt cttccacgag atgcagtggc tcgccgccca
      481 gggctacgtg gtcttctaca ccaatccgcg cggcgggcag gggcgcggcc agacctgggc
      541 cgacgcgatc tccggaggat ggggcgggct ggactaccag gactgcatgg ccgccgccga
      601 cttcctcgcc gcacagccct tcatcgaccc gaaacgcatg ggcgtcaccg gcggctccta
      661 cggcggctac atgaccaact ggatcatcgg acacacggac cgcttcaagg ccgccgtcac
      721 ccagcgctcg gtcgtcaatt tcatgtccat gtacggcgcc tcggatatcg gctggtcgct
      781 cgagcgggag ctcgacggca caccgtggaa caactccggc aactatgaga agtgctcgcc
      841 gatcacctac ttcacgaacg tccgcacccc ggtgctgatc ctccacaatg aacaggacct
      901 tcgctgcggg atcgagcagg ccgagcagat gttcacgatg ctcaagcgtc tgcgccggac
      961 ggtcgagatg gtgcggttcc cggaggagcc ccacggcctc tcgcgccacg gccggcccga
     1021 ccgccgcatc gcccggctca cctggatcgc caggtggttt gacaggtatc tgaagtgatt
     1081 cggcgccgtc cgaatgctgc caagccgtcg cctcgcccga ggcgacggct tttttgatgg
     1141 aaccgctgcc gcccccgcca acagcgcatc agtatacaga atgcgctccc tggacctgac
     1201 caagggggca tgcacggctc cggtcccgcg cacggcctcg acagtttgtg cagtatctga
     1261 gagcaaggtg cgttcttctc tgcatcagct cttcttgcag ctctcccaac actcgctgta
     1321 ccgctaaaag tactgaagtc gttgcagatg gcggtcggcc cccgacgaca tcgctcccca
     1381 gacgaggctg agagacagcg cgttgcccga caggtcgctt ccgccgtggg tgacgtaatc
     1441 gacgatcgtg cagcgccact gcaaccccaa ataccgggtg caacctcggt caaagcagac
     1501 ccgatgagtg aaaccgagcc cgagcgccgg agagttgatc cattgggctt cgttgcggcc
     1561 ggtgcgcgaa accgccctga tgccgtcaaa cccgaccgta gcgaacacgt ccgcggacca
     1621 ccgccttgca cggatcacct cgtatgctgt cgagcacccg acgaacaagc ccaggaattc
     1681 cttcgtttga cgtatctcgc cgcctgcgcg cactcggtac ggttcgcttt ctcccaggaa
     1741 gcgagcagcg aaatccaacc cgcactccca cctgttgtcc cggaccgcaa actgacctcc
     1801 tacttcgagt ttgttcccga gaactttgtt gcctccgaca
//
LOCUS       DAOOKS010000447         1839 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_80823_length_1839_cov_2.375561, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000447 DAOOKS010000000
VERSION     DAOOKS010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80823_length_1839_cov_2.375561"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1343)
                     /gene="hppD"
                     /locus_tag="PK112_07350"
     CDS             complement(<1..1343)
                     /gene="hppD"
                     /locus_tag="PK112_07350"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR01263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HPC11899.1"
                     /translation="MSSPLGIRGYDYVEFYVGSARMTAFWFARALGLQLTGYAGPETG
                     VRDRISFYLTRHRLKFVVTSPLQPDTWDIWGWITKHGDGVKRWAVEVDSVEDAFAYAA
                     KRGAVMVKRPERISNRHGYIDEAAIRLYDDTELVYVNRDHYRHIFRPGFCEPLFSFDA
                     GRDDTGLLAVDHIVANVRENEMNLWAEYFNNTMDFETFVDFGPGDISTRYSSLLSKVV
                     RSKDSKIRNPINEPYEGLKKSQIEEFIEQYRGSGIQHVALRTGDIVATVSALRRNGVE
                     FLAVPDAYYDRLRGRSEVMSRLREDLALLKQHGILCDIESDGYLLQLFTKPIGDRPTF
                     FFEVIQRVGNSEGFGKGNFRALGKRFGKQTPVVAKAIAEAEAAVLAEAVADGGSFALV
                     VSGFDEPVLVGADDVLISERPREGWSVINQQGETVALDLTLTPELWKAHPGLSAFR"
BASE COUNT          337 a          505 c          678 g          319 t
ORIGIN      
        1 cgaaacgcgg acagaccggg gtgggctttc caaagctccg gggtgagggt cagatccaag
       61 gcaacggtct ccccctgttg gttgatcacc gaccatcctt cgcggggccg ctcgctgatc
      121 agtacgtcgt cggcaccgac cagcacaggc tcgtcgaaac ctgatacgac cagggcaaag
      181 gatccaccgt cagccacggc ttcagccagc accgccgcct cggcctcggc gatcgccttg
      241 gcaacgaccg gagtctgctt gccgaaccgc ttgccaagcg cccggaagtt gcccttgccg
      301 aacccctcgc tgttgccgac gcgctggatt acttcgaaga agaaggtcgg gcggtcgccg
      361 atcggtttgg tgaagagctg gagcaggtag ccgtcgctct cgatgtcgca gaggatgccg
      421 tgctgtttga gcagggcgag gtcctcccgc aggcgggaca tgacctcgga gcggccgcgg
      481 agccggtcgt agtaggcgtc ggggacagcc aggaactcga cgccgttgcg acggagggcg
      541 gacacggtgg cgacgatgtc gccggtgcgg agggcgacgt gctggatgcc ggagccgcgg
      601 tactgctcga tgaactcctc gatctgggac ttcttgagtc cctcgtacgg ttcgttgatg
      661 gggttgcgga tttttgagtc tttcgagcgc acaactttcg acagcaggga ggaatacctg
      721 gtgctgatgt cgccggggcc gaaatcgacg aaggtttcga aatccatggt gttgttgaaa
      781 tactcggccc agaggttcat ctcgttctcg cggacgttgg cgacgatgtg atcgacggcg
      841 agcaggccgg tgtcgtcgcg cccggcgtcg aaggagaaga ggggttcgca gaagccgggg
      901 cggaagatgt ggcggtagtg gtcgcggttg acgtagacga gttcggtgtc gtcgtagagg
      961 cggatggcgg cttcgtcgat gtagccgtgg cgattggaga tgcgttcggg gcgtttgacc
     1021 atgacggcgc cccgtttggc ggcgtaggcg aaggcgtcct ccacggaatc gacctcgacc
     1081 gcccagcgtt tgaccccgtc gccatgcttc gtgatccacc cccagatgtc ccaggtgtcg
     1141 ggctggaggg gggaggtgac gacgaatttg aggcggtggc gggtgagata gaaggagatg
     1201 cggtcgcgga cgccggtttc ggggccggca tagccggtga gctggaggcc gagggcgcgg
     1261 gcgaaccaga aagcggtcat gcgggcggag ccgacataga attccacgta gtcgtaaccg
     1321 cgaataccga gcgggctgct catgtcaagt ctcctctcta tcggtttttc gggatcgggt
     1381 cggcaacgca gcgacggccg ccgggggcgg cggcacgggc cgcgggcgcc gctactaacg
     1441 gcaaaaacag gataggcttg ggattgttcc cggccaaacg ggcaccggcg cgcgtggtcg
     1501 gccggcccgg aaaaccgctg ccgggggccg gccgcaggga gcggccggtt tgactatcgg
     1561 cttgacacaa gactattttc agtgtattct aaaaggtcaa cagactggtt cggggcgggg
     1621 cagaagggga aacattccgt ctggcggggg aagccaggcg aggaggaaga ggcggtaccc
     1681 ggatggcggg gagggcggac aggtcggggc cggcgtctca atcggccggc cggattatca
     1741 cggaagttta atgagtacga atcgcacggc aaacgcggcc gccggagcag cccgttggca
     1801 cggaaaccca tgtcgagatc gccgacgacg ttgtggaac
//
LOCUS       DAOOKS010000448         1837 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_80942_length_1837_cov_3.351291, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000448 DAOOKS010000000
VERSION     DAOOKS010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80942_length_1837_cov_3.351291"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1287)
                     /locus_tag="PK112_07355"
     CDS             complement(<1..1287)
                     /locus_tag="PK112_07355"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HPC11900.1"
                     /translation="MTKTKQEILRLMDDGGVRYIRLCFTDILGRIKGMSIPRTEIEQV
                     LEEGQPFDGSSVEGFARIEESDLLAMPDPVTFRIMPWESSGERLAMMFCDIVLPDGTP
                     YDGDPRWVLRRQLQKVAERGWTFYVGPELEYFYLRDERTTDLIDVDGYFDMGTVDKGS
                     QVRKATVNALEAMEIPVECSHHEVAPSQHEIDLKYQEALVMADFAQVYKFVVKQVAMQ
                     HGLYATFMPKPVFGENGSGMHCHQSLFMGDKNLFANGTDQYGLSEIGRQYTAGILKYV
                     REFTLVTNQWVNSYKRLVPGYEAPVYISWGRRNRSSMVRVPAHRVGKERATRIELRSP
                     DPGANPYLAFAAMLAAGLQGIEQCLTLPDPVEENIYDMSEEQKARLNIEVLPNSLENA
                     VRMFEESQLMREVLGEHVFTKLIENKRIEWDQYRIHV"
     gene            1578..>1837
                     /locus_tag="PK112_07360"
     CDS             1578..>1837
                     /locus_tag="PK112_07360"
                     /inference="COORDINATES: protein motif:HMM:NF023807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11901.1"
                     /translation="MTDKIISHPRDFPAVEELLQHPRLADLIARLPRPVAADVVRAVV
                     AEAKSRLTSQDRPLTRDDLLAALRAALRAAGRQAAARVINATG"
BASE COUNT          303 a          654 c          530 g          350 t
ORIGIN      
        1 cacgtggatg cggtactggt cccactcaat gcgcttgttc tcgatcagct tggtgaagac
       61 gtgctcgccg agcacttccc gcatgagctg cgactcctcg aacatccgca cggcattctc
      121 cagcgaattg gggaggactt cgatgttcag gcgggccttc tgctcttccg acatgtcgta
      181 gatattttcc tccaccgggt cggggagcgt caggcactgc tcgatcccct ggagcccggc
      241 cgccagcatc gccgcgaagg cgaggtaggg attggccccc gggtcggggg agcgcagctc
      301 gatgcgcgtg gcccgctcct tgcccacacg gtgcgccggc acccgcacca tgctcgaccg
      361 gttccggcgt ccccacgata tgtacacggg cgcttcgtac cccggcacca gccgcttgta
      421 ggaattcacc cactggttgg tcaccagcgt gaactccctc acgtatttga gaatccccgc
      481 cgtatactgc cggccgatct ccgacagccc gtactggtcg gtgccgttgg cgaacaggtt
      541 cttgtccccc atgaagaggg actggtggca gtgcatcccg gaaccgttct cgccgaaaac
      601 cggcttgggc atgaaggtcg cgtacagccc gtgctgcatc gccacctgct tcaccacgaa
      661 cttgtagacc tgggcgaaat cggccatcac cagcgcctcc tggtacttca ggtcgatttc
      721 atgctgcgag ggcgccacct cgtggtggga gcactccacc ggaatctcca tcgcctccag
      781 cgcattcacc gtcgccttgc gcacctgcga tcccttgtcg accgtcccca tgtcgaaata
      841 cccgtccacg tcgatcaggt cggtcgtccg ctcgtcgcgc aggtagaagt attcgagctc
      901 cggccccacg tagaacgtcc agccgcgctc ggccactttc tgcagctgcc ggcgcaggac
      961 ccaccgcgga tcgccgtcgt agggcgttcc gtcgggcagg acgatgtcgc agaacatcat
     1021 ggccagccgc tcccccgacg actcccaggg catgatccgg aaagtcaccg ggtccggcat
     1081 cgccagcagg tccgactcct cgatccgggc gaatccttcc accgaggatc cgtcgaacgg
     1141 ctggccttcc tccaggacct gttcgatctc cgttcgcggg attgacatac ccttgatgcg
     1201 gccgagaata tcggtgaaac agaggcggat atagcgcacc ccgccgtcgt ccattaatct
     1261 gagaatctcc tgcttcgtct tcgtcatcgc ttgtctcctg gtaccggttt gacgttggtc
     1321 gggtgacccg cccattttgg aagcggacaa taaactagga atggcggtaa aaacaataaa
     1381 aatcctgcgc ggttcggcga aatttccccg caccgtggac aaaagggagt cggtcgaccc
     1441 atgatcccgc cccggtcggg ggctgccccc ggtctgcgcc cggccccggc cctgcttttt
     1501 ccgttgactt gccccccgct ttggcgatta ttgggaggcg ggcgccgccg gcggcgcccc
     1561 gggatccggg taacactatg accgacaaaa tcatttccca tccgcgcgac ttccccgccg
     1621 tcgaggagct cctccagcac ccccgcctgg ccgatctcat cgcccgtttg ccccgtccgg
     1681 tcgccgccga cgttgtccgg gccgtcgtcg ccgaggccaa atcccggctg acgagccaag
     1741 accggccgct gacccgcgac gaccttctcg ccgccctccg ggccgctctc cgtgcggccg
     1801 gccgacaggc cgccgcccgg gtcatcaacg ccaccgg
//
LOCUS       DAOOKS010000449         1836 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_81010_length_1836_cov_3.289163, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000449 DAOOKS010000000
VERSION     DAOOKS010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81010_length_1836_cov_3.289163"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="PK112_07365"
     CDS             complement(<1..629)
                     /locus_tag="PK112_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11902.1"
                     /translation="MRWAAGPAAAAALWLGACGDDAQERAARLFPAGPPIAHLEEFPF
                     NADSLLPGGGAGPEEPPTVRKIGALAVDSSSDPGYLAYDFIGEAAARYTADEAEVRVT
                     IAQFAEPIAAYGWYAAHRPNGAATDTIGRQSFWTGTRLYVYKGSYAIVAEAGDTSAAA
                     RAAAERLAREAANRATGEMRLSPYHILFPMHDRVAASDRYAGYGFLGAPR"
     gene            complement(626..1498)
                     /locus_tag="PK112_07370"
     CDS             complement(626..1498)
                     /locus_tag="PK112_07370"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: protein motif:HMM:NF008978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HPC11903.1"
                     /translation="MIHDILRLVRPAQWLKNGIMVLAIVFAGELAHADKIILMACAIA
                     LYCLLSSAVYTFNDVVDAERDRLHPLKKNRPLAAGRIRVATGAVLAGVLAAAGLVGAW
                     FINGPFFLSAVTFLALNLLYSLWWKHVVILDVLTIALGFVVRAYAGAFAIDVPASTWF
                     LINTLLLALFLGLGKRRHELLYLDKEAQAHRKTLVMYSPYLLDQLIAVVTAAMLVIYM
                     LYTFSTEVMHKLGTDKLFVTIPFVVYGVFRYLYLIHRKDQGGSPTRVLIDDRPILLTV
                     MLWLVTASFVLYVL"
     gene            1623..>1836
                     /locus_tag="PK112_07375"
     CDS             1623..>1836
                     /locus_tag="PK112_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11904.1"
                     /translation="MRKRQLKGAGGHYLIAVNQQAGQFSDRLLRRLTGAIRSQGSHYT
                     VIEPASAADLGATAREYCGLAKAARPA"
BASE COUNT          346 a          650 c          564 g          276 t
ORIGIN      
        1 cggggcgcgc cgaggaaacc gtagccggcg taccggtcgg aggccgccac ccgatcgtgc
       61 atgggaaaca gaatgtgata gggggacaga cgcatctcgc ccgtcgcccg gttcgccgcc
      121 tcgcgcgcga gccgctcggc cgccgcgcgg gccgcggccg aggtgtcccc ggcctcggcg
      181 acgatcgcgt agctcccctt gtacacgtag agcctcgtcc ccgtccagaa cgactgccgc
      241 ccgatcgtgt cggtcgccgc cccgttgggc cggtgcgcgg cataccaccc ataggccgcg
      301 atcggctcgg caaactgggc gatcgtcacc cgtacctccg cctcgtccgc cgtgtaccgc
      361 gccgccgctt ccccgatgaa gtcgtaggcc aggtatcccg gatccgagct gctgtcaacc
      421 gcgagcgcgc cgattttccg gaccgtgggc ggctcctccg gtcctgcccc gccgcccggc
      481 agcagcgagt cggcattgaa cggaaattct tccagatgcg cgatcggcgg ccccgccgga
      541 aagagcctgg ctgcccgctc ctgggcgtca tccccgcagg ccccgagcca gagcgccgcc
      601 gcggccgccg gcccggccgc ccacctcaca aaacgtagag cacgaaactt gccgtcacca
      661 gccataacat caccgtgaga agaatcggac ggtcgtcgat cagcacccgc gtcggcgatc
      721 ccccctggtc cttccggtgg atcaggtaga gataccggaa gacgccgtag acgacaaacg
      781 ggatcgtcac gaacagcttg tccgtcccga gcttgtgcat cacttccgtc gaaaaagtgt
      841 agagcatata aatgaccagc atcgccgcgg tgaccaccgc aatcaactgg tccagcaggt
      901 agggcgagta catcaccagc gttttccggt gcgcctgcgc ctccttgtcc aggtagagca
      961 gttcgtgcct ccgctttccc agcccgagaa acagcgccaa aagcagggtg ttgatgagga
     1021 accaggtcga ggcgggcacg tcgatcgcaa acgccccggc gtaggcccgg accacgaacc
     1081 ccagagcgat cgtgaggaca tcgaggatca ccacgtgctt ccaccacagg ctgtacagga
     1141 gattgagcgc caggaacgtg accgcggaca agaagaacgg gccgttgatg aaccacgctc
     1201 cgaccagccc ggcggcggcc agcacccccg ccagcaccgc ccccgtcgcc acccggatcc
     1261 gcccggccgc cagcggccgg ttctttttca acgggtgcag gcggtcccgc tccgcgtcca
     1321 cgacatcgtt gaacgtgtag acggccgagg acaacaggca gtagagggcg atcgcgcagg
     1381 ccatgagaat gatcttgtcc gcgtgcgcca attccccggc gaacacgatc gccagcacca
     1441 tgatcccgtt cttgagccac tgcgcgggac gaacgagcct gaggatatcg tggatcatct
     1501 cgacgaagga tacgacaagg caaaatccgg ttcaagttga aactgaccgg ccgggacggc
     1561 ccccgctccg cccccggtta ttttcttttg cttgaccgcc cctttgccta tacttaccgc
     1621 aaatgcgaaa acgacagctt aaaggcgccg gcggccatta cctgatcgca gtgaaccagc
     1681 aggccgggca gttttccgac cgcctcctgc gccggctcac gggcgccatt cggagtcagg
     1741 ggagtcacta cacggttatc gagccggcct cggccgccga tctgggagcg acggcgcgcg
     1801 agtactgcgg gctggccaag gccgcccgcc cggcgc
//
LOCUS       DAOOKS010000450         1835 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_81085_length_1835_cov_3.187079, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000450 DAOOKS010000000
VERSION     DAOOKS010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81085_length_1835_cov_3.187079"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="PK112_07380"
     CDS             complement(<1..582)
                     /locus_tag="PK112_07380"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPC11905.1"
                     /translation="MQESAQGPVADRSTYDTWDFFAQAEWTLGRFSLLAGGRFSYVGV
                     DAGRIQDPLQADRVYSLERDWRHAAATGRIMWRPAEEWRIYGGVSHAYRAPNLSDLTR
                     FDIARTNELEIPAPDLEPETFLCYEAGVKTRRGRWSGEVAVFYTAIRDLIIRVPTGEM
                     LEDNYVVTKRNAATGYVTGLEARAAVYLGAGLTV"
     gene            complement(1265..1801)
                     /locus_tag="PK112_07385"
                     /pseudo
     CDS             complement(1265..1801)
                     /locus_tag="PK112_07385"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
BASE COUNT          271 a          662 c          608 g          294 t
ORIGIN      
        1 caccgtaagc cccgcaccga ggtacaccgc cgcgcgcgcc tccagtccgg tcacatagcc
       61 ggtcgcggca ttgcgtttgg tcaccacgta gttgtcctcg agcatctcgc cggtggggac
      121 gcggatgatg aggtcgcgga tggccgtgta gaaaaccgcg acctcccccg accaccgccc
      181 ccggcgcgtt ttcacgccgg cttcatagca gagaaaggtt tcgggctcaa ggtcgggggc
      241 ggggatttcg agttcattgg tgcgggcgat atcgaagcgg gtgaggtccg agaggttcgg
      301 cgcccggtag gcgtgcgaca cccctccata aatccgccac tcttcggccg gccgccacat
      361 gatgcggccg gttgcggccg cgtgccgcca gtcgcgttcc agcgagtaca ctcggtcggc
      421 ctgaagcggg tcctggatgc gcccggcgtc gacccccacg tacgagaatc gccccccggc
      481 cagcaggctg aaccgcccga gggtccattc ggcctgggcg aagaaatccc aggtgtcgta
      541 ggtggagcgg tcggccaccg gcccctgggc cgactcctgc acgagcgccc ccgccgcatc
      601 gtagtccgag gcccacgagg aaaccctgtc ccggtaggtc gatcccgacc gtggcgcggg
      661 cctgcaggag cgccagctcc gtctcgaccg acaagccgac cgtacgcacc gtgcacccct
      721 gccgccgcgc ggtgcggtcc ttcttcaccc ggtcctgttc ctcgttctgg cggtgccacg
      781 acagggtgcc cgagagccgc tggacccggc ccgacaggtc gaacaccgtg taccgcgcat
      841 atcccaggtc gcgctcctgg tccagcacgc accggcggtc gctgccgacg gtcgtgcctt
      901 cccagctgat cccgtagatc gtcttgtgcg cccgccagac atcctcctgc gttgtccgct
      961 ggtatgcgcc cgccagctgg ctgtttcggc gcggcgaata ttcggcgcgc acgtccacat
     1021 tccactcatc gtagccggtt ttcggctgcg cgccgacccc gcctccggcg cggaggtcgc
     1081 cgaagagttt ccccgtgagg ccgaccgcaa ggccgaggtg ttcggccggc gccgcggcga
     1141 gactgatccg gcccgtgtgc gaatcctcgg cgctcgcgta gcggtactgc cagccgggac
     1201 gcgccgtgcg gttcgccagg agcgccgagg gatgcggcgg gatcacattc acgacccccc
     1261 cgaccgcatc ggtgccgtag agcaccgatc ccgggcccat gaccacctcg accgcctcga
     1321 ccgaatggag gtcaaccgtg ctccagtact ggttgggacc ggaacgaaag accgagttgt
     1381 tcaaccggat gccgtcgacg agcaggagcg tgtgaaaccc ggtaagcccc cgcaagtacg
     1441 gagaggcctg gccgtacgcc gttttctgca ccatggcgcc gggaacctcg gccagcgcat
     1501 ccggaagcgt ccgcgccagc cgctcgctcc gcgccgaggc tccgctgagc gaggaggcgg
     1561 cggccggaag atcgagcttg cggtgggcgc tgcgggtggc cgtcacgaga actgtcccca
     1621 gttcgacttc cggcccggcg gcggccgaat ccccggccgg ccggctcgcg gcgacggtgt
     1681 ccgatgaggc gattgtggtg ctctcggcgc cgcgcaccgt tccggcggcg gccagcacgg
     1741 ccgctgccgc aatgatcgtg ccgatactca ctgctctgtt caccttgcct ctgcccttca
     1801 tcacttgcag gcgcgccggt tagagttcaa cccgc
//
LOCUS       DAOOKS010000451         1827 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_81623_length_1827_cov_3.386005, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000451 DAOOKS010000000
VERSION     DAOOKS010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81623_length_1827_cov_3.386005"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="PK112_07390"
     CDS             complement(<1..319)
                     /locus_tag="PK112_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HPC11906.1"
                     /translation="MPGGNFLFTSESVTEGHPDKMCDQISDGVLDEVLRRDPHGRVAC
                     ETFVTVGLVIVGGEITTDAWVDIERLVRGVVKRIGYVDPTLGFSYDSCAILNAIGQQS
                     PDIK"
     gene            complement(370..1356)
                     /locus_tag="PK112_07395"
     CDS             complement(370..1356)
                     /locus_tag="PK112_07395"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HPC11907.1"
                     /translation="MSKRIRVIVPVAGAGTRLQPHTLTLPKALLPVGGKPALAHVLEP
                     LVPLEPEEVVFVIGHLGDQIIDYVRENYRIKARFAAQEKLLGLGYALHLALREMADGP
                     TLVILGDTVVECDYGAFLGAGEYVLGLRSVDNPHRFGVAEVDGGRIVGLEEKPADPRS
                     NLALIGLYYFSDTAQLRKELEYLVRTGKTTGGEIQLTDALHAMIAAGVRFAPYEVARW
                     YDCGKMETLISSNREILKQRATPAAPAGSVFVPPVYAAPTATIINSIIGPNVSIGEAA
                     RIERSVVSNAIIGERSQIVNAVLDHTLIGRDVFLEGTPRRMNVGDKSQVGDV"
     gene            complement(1428..>1827)
                     /locus_tag="PK112_07400"
     CDS             complement(1428..>1827)
                     /locus_tag="PK112_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11908.1"
                     /translation="SFRQPAVDLIAVRLTPAAADSFCLEAYFLPRAKVSADLDITCRL
                     ETPDPVLSAEQQPRFPYDFRPVRPSSMWAPGRIAVGLYRGTFPHPVSAVLIGLHEMRE
                     GRTVALPLGDRSTLCRVPVGASGGTPDPPL"
BASE COUNT          325 a          637 c          574 g          291 t
ORIGIN      
        1 gcttgatgtc cggcgactgc tggccgatgg cgttgaggat ggcgcaggaa tcatacgaga
       61 accccagcgt cggatcgaca tagccgatcc gtttgaccac cccgcggacg agtctctcga
      121 tgtccaccca ggcgtcggtg gtgatctcac cgccgacaat caccagaccg accgtcacga
      181 acgtctcgca ggcgacccgc ccgtgcgggt cgcggcggag cacctcgtcg agcacgccgt
      241 ccgaaatctg gtcgcacatt ttgtcggggt ggccctcggt caccgattcc gatgtaaaga
      301 gaaaattgcc tccgggcatt cgtcagtctc cttcacctca agccggctgt gcctgcgccg
      361 ccgctgcgct cacacgtcgc cgacctgcga cttgtcgccg acattcatgc ggcgcggcgt
      421 tccctcgagg aacacgtcgc ggccgatcag cgtgtggtcg agcacggcat tcactatctg
      481 tgatcgttcg ccgataatgg cgttgctgac aacggaacgt tcgatccgcg cggcctcgcc
      541 gatcgatacg ttcggaccga taatgctgtt gatgatggtc gcggtgggag ccgcgtaaac
      601 cggcggcacg aacaccgacc cggccggcgc cgcgggagtc gcacgctgct tgaggatctc
      661 gcggttcgaa gaaatgagcg tctccatctt cccgcagtcg taccagcgcg ccacctcgta
      721 gggcgcgaac cgcacgccgg ccgcaatcat ggcgtggagg gcgtcggtca actgaatctc
      781 cccgcccgtc gtcttacccg tgcgcaccag gtactccagc tccttccgca gctgtgccgt
      841 atcggagaag tagtacagcc cgatcagcgc gaggttcgac cggggatcgg ccggtttctc
      901 ctcgagcccg acaatccggc cgccgtcaac ctccgcgacg ccgaagcggt gcgggttgtc
      961 caccgaccgc aggcccaaga cgtactcgcc ggcccccagg aacgccccgt agtcgcactc
     1021 gaccaccgtg tcgccgagaa tcaccagcgt cggaccgtcc gccatctccc gcagcgcgag
     1081 gtgcagggca tagccgaggc cgagcagctt ctcctgggcc gcgaaacgcg ccttgatccg
     1141 gtagttctcc cggacgtagt cgatgatctg atcgccgaga tggccgatga caaacaccac
     1201 ttcctcgggc tccagaggca ccagcggctc caacacgtgc gcgagcgccg gcttcccgcc
     1261 caccgggagg agggctttgg gaagggtaag ggtgtgcggc tgcagccggg tgccggcccc
     1321 ggcgaccgga actatgacgc gtatcctctt gctcatgaca aacctgccct atggtagatg
     1381 ccggccaatc cttccgcaag cacaatcttg gaaccccggg gcgctcctca caacggcggg
     1441 tccggagtcc cgcccgacgc ccccaccggg acgcggcaga gcgtcgatct gtcgccgagc
     1501 ggaagcgcca ccgtccgccc ctcgcgcatc tcgtgcagac cgatcagcac cgccgaaacc
     1561 ggatggggga aggtcccccg gtagagaccg accgcgatgc ggccgggcgc ccacatcgaa
     1621 ctcggccgca ccggccggaa atcgtacggg aaccgcggct gctgctccgc gctcagcacc
     1681 ggatcgggcg tctccagccg acaggtgata tccagatccg ccgatacctt cgcgcgcggg
     1741 aggaagtacg cttccagaca aaagctgtcc gccgccgcag gggtgaggcg gacggcgatg
     1801 aggtcgacgg cgggttgccg gaaactc
//
LOCUS       DAOOKS010000452         1827 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_81641_length_1827_cov_2.822799, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000452 DAOOKS010000000
VERSION     DAOOKS010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81641_length_1827_cov_2.822799"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..962)
                     /locus_tag="PK112_07405"
     CDS             complement(<1..962)
                     /locus_tag="PK112_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020532851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase"
                     /protein_id="HPC11909.1"
                     /translation="MVRRLSGKILSGLAGLVALVLLGGCGTGGNEGVAGTGDAAARVY
                     VAPGTYDEFYAFMSGGFSGQVSVYGLPSGRLLRHVPVFSQYAENGYGYSEETKNMLMT
                     SFGFVPWDDAHHPQLSQTDGVPDGRWLFINGNNTPRVARLDLTTFETTEIIELPHSGG
                     NHASPFLTPNTEYVMAGTRFSVPIPQQDVPVSTYKENFKGTLSFIKVDSATGRMSLAF
                     QILVPGFDYDLSHAGKGPSHGWAFFSTYNTEQANTLLEVNASRNDKDYMAAVNWKLAE
                     RYAAEGKAKDVPASYRHNFLDEARVAQSEVVGSVKILDPRDCPG"
     gene            complement(1016..1549)
                     /locus_tag="PK112_07410"
     CDS             complement(1016..1549)
                     /locus_tag="PK112_07410"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HPC11910.1"
                     /translation="MTVEFRKLIGVAGFALTLWLMGCSSGPETEAERQTRWQQGKLTG
                     WELEHGIGPITDALEIGPVDAARAAQGRELFIAKCATCHYLDDRKTGPGLRDVTKRRS
                     PEYVLNQVLNPEQMGKLHPEGKKLVAQYAQFMTIQGITPDDARALLDFLRSEADKPPV
                     PLEQQPGANVPPPPPAN"
BASE COUNT          317 a          642 c          506 g          362 t
ORIGIN      
        1 atccccggac aatcccgggg gtccaggatt ttgaccgagc cgaccacctc gctctgcgcc
       61 acgcgggcct cgtcgagaaa attgtgccgg tacgaggcgg gcacgtcttt agccttcccc
      121 tccgcggcgt accgctcggc cagcttccag ttgacggccg ccatgtagtc cttgtcgttg
      181 cgggaggcat tcacctccag cagcgtgttc gcctgctccg tgttgtacgt ggagaagaac
      241 gcccagccgt gcgatggtcc ttttccggcg tgcgagaggt cgtagtcgaa cccgggcacg
      301 agaatctgga acgccagcga catccgcccc gtcgccgagt ccaccttgat gaacgacagc
      361 gtccccttga aattctcctt gtaggtgctc accggcacgt cctgctgcgg gattggcacg
      421 ctgaaccgcg tccccgccat gacatattcg gtgttgggcg tgagaaaggg ggaggcatgg
      481 ttgccgccgg aatgcggcaa ttcgatgatc tccgtcgtct cgaacgtggt cagatccagc
      541 cgcgccaccc gcggcgtgtt gttcccgttg atgaacagcc accgcccgtc tggaaccccg
      601 tccgtctggg agagttgcgg atggtgcgcg tcatcccacg gcacaaaccc gaacgacgtc
      661 atcagcatgt tcttcgtctc ttcgctgtag ccgtagccat tctcggcgta ctgcgaaaac
      721 accggcacgt gccgcagcag acgccccgag ggcagtccgt agacgctcac ttgccccgaa
      781 aatccccccg acatgaacgc gtagaactca tcgtacgtcc cgggcgccac ataaacccgc
      841 gccgcggcat cgccggtacc ggcgacacct tcgttgccgc cggtaccgca tccgccaagt
      901 aggactaacg caaccagccc ggccaggccg gaaaggattt tcccagataa cctccgaacc
      961 atacttctct ctcctctcac cacatcacga aacgtgctcg gtttcttcgc tccactcagt
     1021 tggccggcgg cggcggcggg acattcgccc ccggctgctg ctcaagcggc accggcggct
     1081 tgtcggcctc cgaccgaagg aaatccagga gcgcccgcgc gtcatccggc gtaatcccct
     1141 ggatcgtcat gaattgtgcg tactgcgcca ccagcttctt cccttccgga tgaagcttcc
     1201 ccatctgctc cgggttgagt acttggttca gcacatactc cggactacgg cgcttggtta
     1261 catcgcgcag tcccggaccg gttttccggt catccagata gtggcaagtc gcgcacttgg
     1321 cgataaagag ctcccgcccc tgcgccgctc gcgctgcatc gaccggaccg atctcgagtg
     1381 cgtccgtgat cgggccgatc ccgtgttcga gctcccagcc ggtcagcttc ccctgctgcc
     1441 agcgggtctg gcgctccgcc tccgtttccg gccccgagga acatcccatc aaccacagag
     1501 ttagagcaaa ccccgcgacc ccgatcaact tcctgaactc taccgtcatg acaaactacc
     1561 tcttctgtcg gttaccggct ggaccccgat tcacacccgg agtctgaccg cctataacaa
     1621 gatttcggag tatgaaatcc gtaatattgc cgtgctcaga ccgctcgcgc ggcctgcgcg
     1681 attatctcct ttttccctcc gccgatcctt tcggcacaag gtcggccagc gtcgtccggg
     1741 tcagcatggc ccgcagatcc tcccgccgct tccgccaggc ctcgtgcagc gggcagggcg
     1801 tcttgtcgct gcactgcggg agtcgca
//
LOCUS       DAOOKS010000453         1826 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_81709_length_1826_cov_3.075099, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000453 DAOOKS010000000
VERSION     DAOOKS010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81709_length_1826_cov_3.075099"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="PK112_07415"
     CDS             <1..360
                     /locus_tag="PK112_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004595068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-CoA thiolase"
                     /protein_id="HPC11911.1"
                     /translation="QAGRKITDVQFAEVHDCFTIAELVVLESMGVYKPGGAGPATLAG
                     ETALDGKFPVNPSGGLKSKGHPVGATGIAQAVEVFKQLTRQAEGGRQIKGNLRIGMAQ
                     NMGGSGGSSVVHIMEVA"
     gene            360..761
                     /locus_tag="PK112_07420"
     CDS             360..761
                     /locus_tag="PK112_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HPC11912.1"
                     /translation="MAMFSSRNWREYPQRYRLEAVRFKKSGKTYFPPRSIDPENRDTE
                     RETVRLPDTGKIVTYTVVRVAPSQWGDMAPYALAIVQLTDGTRLFGQMTDCNVDEVKI
                     GMEVRIEFRRIQTEEHHGVLSYGYKFVPKWW"
     gene            959..1459
                     /locus_tag="PK112_07425"
     CDS             959..1459
                     /locus_tag="PK112_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11913.1"
                     /translation="MYMLGAVAGDIAREIAQIKQEAAGRGIRVMRGRLIGPGGAEAPR
                     VVWVADGDGDWREFLDFAQSVGVKAVVIDFDRIDMELLRADVEDAAVDEEYEEGRTKM
                     LQMLEKLDKHDGEIGHVGIIFPHGPLVYQASVRTPWYDDYVTVREIAGAADEFAEEEE
                     DPFDIQ"
BASE COUNT          345 a          522 c          671 g          288 t
ORIGIN      
        1 caggccgggc ggaagatcac cgacgtccag ttcgccgagg tccacgactg tttcacgatc
       61 gcggaactcg tggtgctcga gtcgatgggc gtgtacaagc cgggcggtgc cggtccggcc
      121 accctggccg gggagacggc gctggacggc aagttcccgg tcaacccctc gggcggcctg
      181 aagtcgaagg ggcacccggt cggagccaca gggatcgccc aggcggtcga ggtgttcaaa
      241 cagctcacgc gccaggccga aggcggccgc cagatcaagg ggaacctccg cattgggatg
      301 gcgcagaaca tgggcgggtc cggcggttcc tcggtcgttc acatcatgga ggtggcgtga
      361 tggctatgtt cagttcgcgc aattggcgcg agtacccgca gcggtaccgc ctcgaggccg
      421 tgagattcaa aaagagcggc aagacctatt tcccgccgcg ctcaatcgac cccgaaaacc
      481 gcgacaccga gcgcgagacg gtgcggctcc ccgacaccgg gaagatcgtc acctacacgg
      541 tggtgcgggt ggctccctcg cagtgggggg acatggcccc ctatgccctg gcgatcgtgc
      601 aactgaccga cggcacccgg ctgttcgggc agatgaccga ctgcaacgtg gacgaggtga
      661 aaatcggaat ggaagtgcgg atcgagttcc gccggatcca gaccgaggaa caccacgggg
      721 tgctgagcta cgggtacaag ttcgtgccga agtggtggtg aggccgttcg ggaattgaca
      781 ccaggcgccg ccgggcggaa acgcccggcg gtttcttttg cggctcacga ggacagaagg
      841 cgcccggccg tcaacttcga ttgcgccgcc gcccgcgccg cggtatattc cccgtcggct
      901 tcaggacagc gcccgaccac gaaacgcctc tagcgagagc aggaaagcga ggaatcagat
      961 gtacatgctg ggagcagtcg ccggggacat cgcccgggag atcgcgcaga tcaagcagga
     1021 ggccgccggc cgggggatcc gggtgatgcg cggccgcctg atcggacccg gcggggcgga
     1081 ggcgccccgc gtcgtctggg tggcggacgg agacggcgac tggcgggagt ttctcgattt
     1141 cgcgcagagt gtgggcgtga aagcggtcgt gatcgatttc gaccggatcg acatggagct
     1201 cttgcgggcg gatgtcgagg acgcggcggt cgacgaggag tacgaagagg ggcgcacgaa
     1261 gatgctccag atgctggaga aactcgacaa gcacgacggg gagatcgggc atgtcgggat
     1321 catattcccg cacggaccgc tggtctatca ggcctcggtg cggacgccgt ggtacgatga
     1381 ctacgtgaca gtgcgggaga tcgccggcgc cgcggatgag ttcgcggagg aagaggaaga
     1441 ccctttcgat atccagtgac gtgcgggcgg gggctccggc cgctctgctt cgcgccgcgg
     1501 cccgaagggc gttgacagaa cgaggagggg atagtatact attgaatgcg ccccggcagc
     1561 tcgcgattgg tgaatctcgg gattgcagtg aagcggcgat tagtcccggc cccttgggcg
     1621 cagggtacac agggattgac agcggacaga catatatcat tatatcctat cttatccgcg
     1681 ggtcgggagc gtttggccga gggcggctcg ttcgttcccg gcgcgcgccg caggctgatt
     1741 aaccgatacc ggagagcggc gacgcagcgg gtgaaacgcg caggaggtct tcatggtccg
     1801 aagtgtggcg aggtttgtgg cgccgg
//
LOCUS       DAOOKS010000454         1823 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_81922_length_1823_cov_2.906109, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000454 DAOOKS010000000
VERSION     DAOOKS010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81922_length_1823_cov_2.906109"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1823
                     /locus_tag="PK112_07430"
     CDS             <1..>1823
                     /locus_tag="PK112_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11914.1"
                     /translation="VARANAEMSREELALLRDSILAALPEGYRDTEFLTQRLDSVMTA
                     YDEVRLRWPNVQEARKALRDGLDDGALSSGEADKLSVAIERARMRFIPEEVTIPYTVV
                     FDGEPTAIAAKEKLLVVGTSEGLFTYDGKSWRTFTEKDGLPSDEIRSLHASGAQFFVG
                     TAEGLVMFTGRDIAAVDTTGTLPAGYVSAIGADGTSRVWAVIDGKLYRYDGASWSRGR
                     AYTIAVGDTLEKIAERFALYGTASERQSLVDLIKELNPRGPQAPAAAAAAAPAQGEPL
                     DITKLAEMAADTAAADSAAPAAETAAAGEAATAEEAPAAESGGEPDWANLPPGDVLIL
                     PFAVEIKGNVNSIAARQDELFLGTDFGLLYFDGEKWRMPGWSEYTAPQNQTVEDVLGQ
                     RAATNGEARERYLLAIREVNGIESTVDSGAAIRIPQNALAAKVNLVRQRGASVYFATA
                     EGLRVLEDGRVQRIDEKGMGEANTIYMDAVEDELWLASDREIVIKANGRKEFSLMHVN
                     WLPELASDMYYDFIAGVFQVGDVGTAGFNVTYLTYGEITNTNSLGQVIGSFRSFDVAV
                     TGSFGFPVSQKLKVGVSAKLIHSHLSDVGTEKEKGSGTSTG"
BASE COUNT          319 a          537 c          708 g          259 t
ORIGIN      
        1 ggtcgcgcgg gccaacgccg agatgtcgcg cgaggaactg gcgctgctgc gggacagcat
       61 cctggccgcg ctccccgaag ggtaccgcga caccgagttc ctgacccagc ggctcgactc
      121 ggtgatgacg gcctacgacg aggtacggct gcgctggccg aacgtccagg aggcgcgcaa
      181 ggcgctccgc gacgggctgg atgacggcgc gctctcgagc ggcgaggcgg acaagctgag
      241 cgtcgccatc gaacgcgccc gcatgcgctt tatcccggaa gaggtcacga ttccctacac
      301 ggtggtgttc gacggcgagc cgaccgcgat tgcggccaag gagaagctcc tcgtcgtggg
      361 aacctcggaa ggactgttca cctacgacgg aaagagctgg cggacgttca cggaaaagga
      421 cgggctgccc tcagatgaaa tccggtcgct gcacgcgagc ggcgcgcagt tcttcgtggg
      481 gacggcggag gggctggtga tgttcacggg gcgcgatatc gcggccgtcg acacgaccgg
      541 cactctcccg gcgggctacg tctcggcgat cggggccgac ggcacgtcgc gcgtgtgggc
      601 ggtgatcgac ggcaagctgt accggtatga cggcgcctcg tggagccggg gccgggccta
      661 cacgattgcg gttggcgaca cgctggagaa gattgcggag cgctttgcgc tctacgggac
      721 ggcctcggag cgccagtcgc tcgtcgatct gatcaaggag ctgaacccgc ggggcccgca
      781 ggcgccggcc gcggcggcgg ccgcggcgcc ggcccagggg gagccgctcg atatcactaa
      841 actggccgag atggcggcag acacggcagc ggccgacagc gcggcgccgg cggcggagac
      901 ggccgcggcg ggcgaggccg cgacggcaga ggaggctccg gcggccgaga gcggcggcga
      961 gccggactgg gcgaatctgc cccccgggga cgtgctcatt ctccccttcg cggtggagat
     1021 caaaggaaac gtgaacagca tcgcggcgcg gcaggacgag ctgttcctcg ggaccgactt
     1081 cgggttgctg tatttcgacg gcgagaagtg gcggatgccg gggtggagcg agtacacggc
     1141 gccgcagaac cagacggtcg aggatgtcct gggccagcgg gcggcgacca acggcgaggc
     1201 ccgggagcgg tatctcttgg cgatccggga ggtcaacggg atcgaatcga cggtggactc
     1261 cggcgcggcg atccggattc cgcagaacgc cctggcggcg aaggtgaacc tggtgcggca
     1321 gcgcggcgcg agcgtctatt tcgcgaccgc cgaggggctg cgcgtactgg aggacgggcg
     1381 ggtccagcgg atcgacgaga aagggatggg ggaagcgaac acgatctaca tggacgcggt
     1441 ggaggatgag ctgtggctgg ccagcgaccg ggagatcgtg atcaaggcca acggccggaa
     1501 agagttctcg ctcatgcacg tgaactggct gccggaactg gccagcgaca tgtactacga
     1561 tttcatcgcc ggcgtcttcc aggtggggga tgtcggcacg gccgggttca acgtgaccta
     1621 cctgacctac ggggagatca ccaacacgaa ctcgctcggc caggtgatcg ggtcgttccg
     1681 ctcgttcgac gtggcggtga ccgggtcgtt tgggttcccg gtgtcgcaga agctgaaagt
     1741 gggggtgagt gcgaagctga tccactcgca cctgtcggat gtcgggaccg agaaggagaa
     1801 gggttcgggg acctcgacgg ggg
//
LOCUS       DAOOKS010000455         1821 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_82051_length_1821_cov_3.914496, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000455 DAOOKS010000000
VERSION     DAOOKS010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82051_length_1821_cov_3.914496"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="PK112_07435"
     CDS             <1..462
                     /locus_tag="PK112_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11915.1"
                     /translation="MGTACRDDYYLPAPPSIEGCYTGTYTYVSQSGSEVVVSKEQVIT
                     ASFTETKFFLDADTLAEGYNKIACFCVGRFFYSISDRVQIEPIDDRPLIGPGCDSCDP
                     DESPTGTFQLYRPGNDVKLECIETVTSDGGSQLKVTKTLMLTRVACPSTGA"
     gene            968..>1821
                     /locus_tag="PK112_07440"
     CDS             968..>1821
                     /locus_tag="PK112_07440"
                     /inference="COORDINATES: protein motif:HMM:NF024931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HPC11916.1"
                     /translation="MLAKIFSASTFGVDAYTVEVEADIQQQLPAFVTVGLPDGAVRES
                     KERVTSAIKNSDFVFPAKRVTINLAPADIKKEGSAFDLPIAVGIFAATGQILRDRFDE
                     YVMLGELSLDGALRAVPGVLPMAMNFHTGNGIKGIIVPKDNAREAAMADSVPVYPVDS
                     LKDVIAFLEDESSIAPFKVDIESVFNEAYRYAIDFADVKGQEAAKRALEVAAAGGHNI
                     IMIGPPGSGKTMLARRVPTILPDLTIEEALETTKIHSVAGRLPTGAALIATRPFRSPH
                     HSISYAGLV"
BASE COUNT          338 a          588 c          537 g          358 t
ORIGIN      
        1 atgggtacgg cctgccgcga cgattactac ctaccagctc cgccaagtat cgaagggtgc
       61 tataccggca cgtacacgta cgtttcccaa agcggcagcg aggtggtcgt atccaaggag
      121 caggtgatca cggcttcctt cactgaaacc aagttctttt tggatgccga cacattggcg
      181 gaaggctaca acaagatcgc ctgtttctgc gtgggtcgct ttttctattc catctcggac
      241 cgcgtgcaaa ttgagccgat cgatgatcgg ccgctcatcg gaccgggctg cgactcgtgt
      301 gatccggatg aatcgccgac aggcacgttt cagctctatc ggccgggcaa tgacgtgaaa
      361 ttggagtgca ttgagacggt gacgagtgac ggcggctcgc agttgaaggt cacgaagact
      421 ctgatgttga cgcgggtggc atgtccgagt accggagcgt agcagcattc gtacagatgg
      481 cggtcctttt cgagtttgac ggcaggcggt gagcctgccg ttttttttga cggcgtcacg
      541 tctcacgtca agcggcgggc cgccacaagc gcggccgggg ggccggactg ggtgacgccc
      601 cgcctggatg ttgttgctgt acttgcggta aacatcacat caggcacgcg ccccggcaga
      661 cttttacgag tggctgggcc gcacccgcct tcccggccgc gggtgagcgg gcgctacccg
      721 gttcgcacat ggcttcaatg acagccgggg cccactccgg tccacccgac cagagccgaa
      781 aaaagtgatt tctcttcgac tgctcctgct gcataatgtc actataatga tgtaacttgt
      841 cccaggttaa accagcgttg tcctggtccg gcgcgcccgg tacgcacact cgatcctgtt
      901 gggcgcgccc ttgagagccc agggacgcca cgaagcggcg ccccggcgcc gggggaggga
      961 ttcagaagtg cttgccaaga tcttctcggc atcaaccttc ggggtcgacg cctatacggt
     1021 cgaggtcgag gcggacattc agcagcaatt gcccgctttt gtcaccgtcg gtctgcccga
     1081 tggcgcggtg cgcgagtcca aagaacgcgt cacctcagcc atcaagaact ccgatttcgt
     1141 gtttcccgcc aagcgcgtca cgatcaatct cgccccggcc gacatcaaga aagaaggctc
     1201 tgctttcgac ctgccgatcg cggtcggcat tttcgcggcc accggccaga tactccgcga
     1261 ccggttcgac gaatacgtca tgctcggcga gctctcgctc gatggtgcgc tgcgcgctgt
     1321 tcccggcgtg ctccccatgg ccatgaactt ccacaccggc aacgggatca aagggatcat
     1381 tgtccccaag gacaacgccc gcgaggcggc catggccgac agcgtccccg tctacccggt
     1441 cgattcgctc aaagacgtca tcgccttcct cgaggacgag tcctcgatcg ccccgttcaa
     1501 ggtcgacatc gagtcggtct tcaacgaagc ctaccggtat gcgatcgact ttgccgatgt
     1561 caagggccag gaggcggcca agcgggccct ggaggtggcc gcggccggcg gccacaacat
     1621 tatcatgatc ggccctcccg ggtcaggcaa gaccatgctg gcgcgccgcg taccgaccat
     1681 cctgcccgac ctcaccatcg aagaagcctt ggagaccacc aagattcatt cggtcgccgg
     1741 ccgcctgccc accggcgccg ccctcatcgc cacccgcccg tttcgctcgc cgcaccattc
     1801 gatttcctac gccggtctcg t
//
LOCUS       DAOOKS010000456         1818 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_82303_length_1818_cov_2.288712, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000456 DAOOKS010000000
VERSION     DAOOKS010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82303_length_1818_cov_2.288712"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1091)
                     /locus_tag="PK112_07445"
     CDS             complement(<1..1091)
                     /locus_tag="PK112_07445"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HPC11917.1"
                     /translation="MRICHLGDSHLGAGANHPRRGASGLTLRQEDIIAAFAEAVDKVI
                     ALRPDVCIHAGDLFDAVRPLNRVMAAAGELLHRLAEEAGIPTVLISGNHDAPKQPHVG
                     APIDVYRQIDNLYIASAGRREVFRIGEAAVHALPHCLTGPILQEELAQCRPEASARYN
                     VFVGHGVVAGMPEFAMADLGEQEIEVGKLEGFDYIALGHYHNNCEVGKRAWYCGSTER
                     LSQAEREAEKGFLEVVLDPFEVRFHRVHARPMVDIPPIDATGKRGDQLAEMIRAQVTA
                     AGAEDKIIRVRVEGVTEETLKTMPSDVLMELKQQSWALDLTFQKAKAEGAGEAFGRAA
                     IGRLDQEFLRFLESTEIRGFDRDRLREMG"
     gene            complement(1251..>1818)
                     /locus_tag="PK112_07450"
     CDS             complement(1251..>1818)
                     /locus_tag="PK112_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPC11918.1"
                     /translation="QAMKEGSFRVDLFYRLKVFSIYLPPLRDRRSDVPVLLDHFIRKF
                     SKQLHRPPKPVSKRAMQMLVKHHWPGNVRELENNIQTALVIAKGDELECEDFPIFSES
                     TGRVEVNLNDLQEDYTETFRRIIEPVMPKLINNNPGQVFHFLESAFERAVIASCLKEF
                     EGNQVKTSEALGISRNTLRDRISRYGIY"
BASE COUNT          290 a          652 c          530 g          346 t
ORIGIN      
        1 aaccccatct ccctgagccg atcccgatca aaaccgcgaa tctcggtgct ctccagaaac
       61 cgcagaaact cctggtcgag ccgtccgatc gccgcccgcc cgaacgcctc cccggcgccc
      121 tctgctttcg ccttttggaa cgtcagatcc agcgcccatg attgctgctt cagctccatc
      181 agcacgtcgc tcggcatcgt cttgagcgtc tcctccgtca ccccttcgac gcgcacccgg
      241 atgatcttgt cctcggcccc ggccgcggtg acctgcgccc ggatcatctc ggccagttga
      301 tccccccgtt ttcccgtggc gtcgatcggc gggatatcca ccatcggccg ggcgtgcacg
      361 cggtggaacc gcacctcgaa cggatcgagc acgacttcca gaaacccctt ctccgcctcc
      421 cgttccgcct gcgacaaccg ctccgtcgat ccgcagtacc acgcccgttt gcccacctcg
      481 cagttgttgt ggtagtggcc gagcgcgata tagtcgaacc cttcgagttt cccgacctca
      541 atctcctgct cgccgagatc cgccatcgcg aactccggca tccccgccac gactccgtgg
      601 ccgacaaaca cgttgtaccg cgcggacgcc tccggccggc actgcgcgag ttcctcctga
      661 aggatcggcc cggtcaggca gtgcgggagc gcgtgcaccg cggcctcccc gatccggaac
      721 acctccctcc gccccgccga cgcgatgtac aggttgtcga tctgccggta tacgtcgatc
      781 ggcgctccga cgtgcggctg tttgggcgca tcgtggttcc cgctgatcag gaccgtggga
      841 atccccgcct cttccgccag ccggtgcagg agctcccccg ccgccgccat gaccctgttc
      901 agcggtcgca ccgcgtcgaa caagtcgccg gcgtgaatac acacatccgg gcgcagcgcg
      961 atcaccttgt ccaccgcctc ggcgaaggcg gcgatgatgt cctcctggcg gagcgtcaac
     1021 ccgctggccc cccggcgcgg gtggttggct cccgccccga ggtggctgtc tcccagatgg
     1081 catatcctca tgccgggaat atacgccagg agcggctgac gggcaactgc gccctctggc
     1141 ggcgccgacg gccgctcggc ggaagcgccc gggctcccgt ccaaagaaaa ccggctgcct
     1201 ggaccggggg ccgagcagcc ggatgcaatc gctttggccg cgtgcggggt ttagtaaatt
     1261 ccgtacctcg agatccggtc ccgcagcgtg ttacgcgaga tccccagggc ctcggaggtt
     1321 ttcacctggt tcccctcgaa ttccttgagg cacgaggcga tcaccgcccg ctcgaacgcc
     1381 gattcgagga aatggaagac ctggccggga ttattgttga tcagcttagg catcaccggt
     1441 tcgatgatcc gccggaaggt ttcggtgtag tcttcctgaa ggtcgttgag gttgacttcc
     1501 acgcgcccgg tgctctcgct gaagatcggg aaatcttcgc actcgagctc gtcccccttg
     1561 gcgatcacca gcgccgtctg gatgttgttc tccagttccc gcacgttccc cggccagtgg
     1621 tgcttcacga gcatctgcat cgcccgtttc gacaccggtt tggggggccg gtgcagctgc
     1681 ttggagaact ttctgatgaa gtggtcgagc agcaccggca cgtccgagcg ccggtcgcgc
     1741 agcggcggca gatagatcga gaatactttc aaccggtaga agagatcgac ccggaacgac
     1801 ccctctttca tcgcctgg
//
LOCUS       DAOOKS010000457         1816 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_82423_length_1816_cov_3.378194, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000457 DAOOKS010000000
VERSION     DAOOKS010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82423_length_1816_cov_3.378194"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1816
                     /locus_tag="PK112_07455"
     CDS             <1..>1816
                     /locus_tag="PK112_07455"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HPC11919.1"
                     /translation="YAAEAARREDSIALVLNMDMIGYHENDSAVWVYHTVPGSYGALW
                     GALADSLAGVDLSAHLAAGAAWDAEPFEQRGYEALTLHEYVRMPFVHTARDSAVYINF
                     DYLARVVRGTLATTLVAAETFAPGLLRFALPGPLADPLPAGRPIELEVYLRAAGSGCP
                     AAGGARLHYSFGEGAWDSLSMAESGADRYRAELPPVYEMTPLHFYFTATDTSGSVFAY
                     HDREAPFLAVVAARSEVVFRDDFNQDCGWQVMGGATEGVWERVLPQSAAFWGAPGRDY
                     DNSGYCFLTGNATVEDVDGGTTMLVSPWIAVGAGEARIRYARWFADYYPEGEPYSDTF
                     RVYVRPSPSQSFRLVETVGPVVQADGGWYAREFMAGEIVATTGALQLRFDASDAGADS
                     HVEAAVDDITVWRYTCDPAVLTASLPDWTAGVPYSCSLAAAACCSLLVWTDKNDALAG
                     SGLVLEPSGALSGVPLAAQAINLTAQAVDDSGRVGERTFPFTINPVLEVATDWLPRGM
                     VGAAYSRRLEAEGGTGGRVWRDRDLDLAGSGLALDSSGLLSGTPVAAGGYSFVAEVVD
                     QVGARAVRELAVAVDESYVCGDVSGDGVGPNIQDLSFLV"
BASE COUNT          239 a          582 c          675 g          320 t
ORIGIN      
        1 actacgccgc ggaggcggcg cggcgggagg acagcatcgc gcttgtgctc aacatggaca
       61 tgatcggcta ccacgagaac gactcggccg tctgggtcta tcacaccgtg cccggttcgt
      121 acggagcgct ctggggagcc ttggccgatt cgctggcggg ggtcgacctc tcggcgcacc
      181 tggcggccgg cgcggcgtgg gatgccgagc cgttcgagca gcggggatac gaggccctca
      241 ccctccacga gtatgtccgt atgccgtttg ttcacaccgc gcgcgacagc gccgtgtaca
      301 tcaatttcga ctacctcgcg cgcgttgtgc gggggacgct ggcgacgacg ttggtggccg
      361 ccgagacctt tgcgccgggg ctgctccgat tcgcgctgcc ggggccgctg gccgacccac
      421 tcccggccgg ccgtccgatc gaactcgagg tgtatctgcg ggccgccggc tccggctgtc
      481 cggcggccgg gggcgcccgg ctgcactact cgttcggcga gggggcgtgg gactcgctct
      541 cgatggccga gagcggcgcc gatcgctacc gcgccgagct gccgcccgtg tacgagatga
      601 cgccgctgca cttctacttc acggccaccg acacctcggg gtcggtgttc gcctatcatg
      661 accgcgaggc gcccttcctg gcggtggttg ccgcccggtc cgaggtcgtc tttcgcgatg
      721 acttcaatca agattgcggg tggcaggtaa tgggcggggc gaccgaaggg gtgtgggaac
      781 gggttctgcc gcagtcggca gccttctggg gggcgcccgg acgcgactat gataactccg
      841 ggtactgttt cctgaccggt aacgccacgg ttgaagacgt ggacggcggc acgacgatgc
      901 tggtgtctcc ctggattgcg gtcggcgcgg gcgaggcgcg gattcggtac gcccgctggt
      961 ttgccgacta ctatccggaa ggcgagccgt acagcgatac cttccgcgtc tatgtccggc
     1021 ccagccccag ccagtcattt cggctcgtgg agacggtcgg cccggtggtg caggcggacg
     1081 gcgggtggta cgcgcgtgag ttcatggccg gggagatcgt cgcgacgacc ggtgctctgc
     1141 agctccggtt cgacgcctcc gatgccggcg cggactccca cgtcgaggcg gcggtggacg
     1201 acatcaccgt atggcgctac acctgcgatc ccgccgtgct gaccgcatcc ctgcctgatt
     1261 ggaccgcggg tgtcccctat tcctgctcgc tggcggcggc ggcctgctgc agcctgctgg
     1321 tgtggacgga caagaacgac gcgctggccg gcagcgggct ggtcctggag ccctcgggcg
     1381 cgctctcggg agtgccgctc gcggcgcaag cgatcaatct gacggcgcag gcggtcgatg
     1441 acagcgggcg ggtcggggag cgcacgttcc cgttcacgat caaccctgtc cttgaggttg
     1501 cgaccgattg gctgccgcgc ggcatggtcg gcgcggccta cagccgccgg ctcgaagcgg
     1561 agggcggaac cggggggcgg gtgtggcgcg accgggatct tgatttggcc ggcagcggcc
     1621 tggccctgga ctcgtcgggg ctgctgagcg ggacgccggt cgccgccggc gggtattcgt
     1681 tcgtggcgga agtggttgat caggtcgggg cccgcgctgt cagagaactc gccgtggcgg
     1741 tcgacgagtc ctacgtctgc ggcgatgtca gcggcgacgg cgtcggaccg aacatacagg
     1801 atctctcgtt tcttgt
//
LOCUS       DAOOKS010000458         1814 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_82552_length_1814_cov_2.777146, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000458 DAOOKS010000000
VERSION     DAOOKS010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82552_length_1814_cov_2.777146"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1814
                     /gene="ccsA"
                     /locus_tag="PK112_07460"
     CDS             <1..>1814
                     /gene="ccsA"
                     /locus_tag="PK112_07460"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HPC11920.1"
                     /translation="RMHHHPRVLFFTGMMLKVSPFALLDFSAADGAGLNPLLQDPWMV
                     VHPPVMFVGYALTGAPFALALAALVRRDFSEWVRRAAPWVVLTVLFLAAGNILGGYWA
                     YKTLGWGGYWAWDPVENSSLIPWFVALALLHGLALERRTGALRRTNLLLAAFTFWLVV
                     CGTFMTRSGVLSDFSVHSFVDLGQNEWLIVFLLLYAGVTLGVFLRGMRSTGYVPINFN
                     FFGREFSLVGAMLLLFAFSLIVLFWSSLPILTKIFGAQPRAADIATYNAFALPIAVLY
                     ALLLILSPLSSHRPQAPAKWRIHLTAGGLVSLAVALMLFVLHEGSGVAFVVAGTLVAT
                     GLTVAFGQRDLRGSLIPAAALFVLALATALALGVRDGLYLLFFATAAAAVGSNAAAVA
                     RRLPGEWLKAGAHLTHFGFGLMLLGVLASSAFSTGERVTLPQGQPRPAYHLQVSYEGM
                     QNDIKWPHNELILKVVDDGRVDEGRPELYFSERMNGYMKRPYIRRGLLYDLYFSPQDI
                     KQQEGQDGLLIGKAEKASAGGYEFSFLGFDMGDHSGGGEMTVTALIAAARDGRVDTLR
                     PAVTMATADDGSTALQDLPAAFGSGDSTFTMSITRIHA"
BASE COUNT          229 a          626 c          599 g          360 t
ORIGIN      
        1 ggcggatgca ccaccatcca cgggtcctgt tcttcaccgg catgatgctg aaagtatccc
       61 ccttcgccct gctggatttc tctgcggccg acggggcggg gctcaatccg ctgctgcagg
      121 acccgtggat ggtggtgcat ccgccggtga tgtttgtcgg gtatgctctc accggggcgc
      181 cctttgcgct ggcgctggcg gcgctggtgc ggcgcgattt cagcgagtgg gtgcgccgcg
      241 ccgcgccatg ggtcgtcctc accgtgctgt tcctcgcggc cggcaatatc ctgggcggct
      301 actgggccta caagacgctc gggtggggcg gctactgggc ctgggacccg gtcgagaatt
      361 cctccctcat cccctggttc gtggcgctgg cgctcctgca cggcctggcg ctggagcgcc
      421 gcaccggcgc gttgcgccgc accaacctcc tgctcgcggc cttcacgttc tggctcgtcg
      481 tctgcggcac cttcatgacc cgctcgggcg tcctgtcgga cttctccgtc cacagcttcg
      541 tggacctcgg tcagaacgag tggctcattg tcttcctcct gttgtatgcg ggggtcacgc
      601 tcggggtgtt cctgcgcggg atgcgcagca ccggctacgt gccgatcaat ttcaactttt
      661 tcggccgcga gttctcgctc gtcggggcga tgctgctgct gttcgccttt tcgctgatcg
      721 tcctgttctg gtcatcgctg ccgatcctga cgaagatttt cggcgcgcag ccgcgcgcgg
      781 ccgacatcgc cacctataac gctttcgccc ttccgatcgc ggtgctttat gccctcttgc
      841 tgatcctctc gccgctctcg agccaccggc cacaggcgcc ggcaaagtgg cggattcacc
      901 tcactgcggg cgggttggtc tcgctcgcgg tcgccctgat gctcttcgtc ctgcacgagg
      961 ggtccggggt cgctttcgtg gtcgcaggca cgctcgtggc caccggcctc accgtcgcgt
     1021 tcggccaacg cgacctgcgc gggagtctga ttcccgcggc ggcgctgttc gtgcttgcgc
     1081 tggcgaccgc gctggcgctg ggcgtgcgcg acggcctcta cctgctgttc ttcgccaccg
     1141 cggcggccgc ggtcgggtcg aacgccgccg ccgtggcgcg ccggctgccc ggcgagtggc
     1201 tgaaagccgg cgcccacctc acccacttcg gtttcggcct catgctcctg ggcgtgctcg
     1261 cctcctcggc cttctcgacc ggcgagcggg tcacgctccc gcagggacag ccgaggccgg
     1321 cctaccatct ccaggtcagc tacgaaggga tgcagaacga catcaagtgg ccgcacaacg
     1381 agctgattct gaaggtggtg gacgacggcc gggtcgacga ggggcggccg gagctctact
     1441 tctccgagcg catgaacggg tacatgaaac ggccgtacat ccggcgcgga ctcctgtacg
     1501 acctgtactt ctcgccgcag gacatcaagc agcaggaggg gcaggacggg ctgttgatcg
     1561 gcaaagcgga gaaagcctcg gccggcggat acgaattctc gtttctcggt tttgacatgg
     1621 gcgaccacag cgggggcggt gagatgaccg tgaccgcgct gatcgccgcc gcccgcgatg
     1681 gccgtgtcga cacgctccgc ccggccgtga ccatggccac ggccgacgac ggcagcaccg
     1741 cgctgcagga tctcccggct gcgttcggct ccggggactc gactttcacg atgtcgatca
     1801 cgcgcatcca cgcc
//
LOCUS       DAOOKS010000459         1811 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_82808_length_1811_cov_2.460706, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000459 DAOOKS010000000
VERSION     DAOOKS010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82808_length_1811_cov_2.460706"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1453)
                     /locus_tag="PK112_07465"
     CDS             complement(<1..1453)
                     /locus_tag="PK112_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11921.1"
                     /translation="MFRYSRLWVFPAVLFSAVLFLYCGNDDTVTEPEPPRPVIVTGAI
                     DLPPESPLAPEDLTVLCGGGQGAVDSSGRFTAPVWEHSAAALLAVDTDHNPVLMGIVT
                     DPTAKGPVLLDARSTALALVFLNPFVCMADAAEADSILDALEAQVAFADFAALIGRKL
                     EADGLALTVDDPEIDSLLTEVVAEYVNSFVAEGVRSASLAPTPAAPTSPSEAPPTIQP
                     DYERSGHRLTWLGGTSFEISNSFGRWAYCCTPTDSFYVFPNGDFLDWVRKGQPWPPSR
                     RRLTLPVSVAETTVVNIYGYGCLGVADNRWEDLTPAEQLCAHYGGFVTVTLELCSHVL
                     GTVCNTARVAGKDAIAERINESILKPIFTDGRVYQRVAAYMEANDPWGCSWFLTKEVI
                     KKIVTSPGYREAFCIITGITLTDGMISTLASWLAIPAKVTLTFNSISSAFKTALALSS
                     ARYKTTFKVWKENAEFGNIEGMVADKTHGTGIAG"
BASE COUNT          320 a          602 c          593 g          296 t
ORIGIN      
        1 ccccggcaat ccccgtcccg tgggtcttgt ccgccaccat cccctcgatg ttgccgaact
       61 cggcgttctc cttccacact ttgaacgtcg tcttgtagcg ggccgaactc agcgcgaggg
      121 cggttttgaa agccgacgat atcgagttga acgtcagcgt caccttggcc gggattgcca
      181 gccacgacgc gagcgtgctg atcatcccgt cggtgagcgt gatcccggtg atgatgcaga
      241 acgcctcccg gtagcccggc gaggtgacga tcttcttgat cacttccttg gtcagaaacc
      301 atgagcagcc ccaggggtcg ttggcctcca tgtaggcggc gacccgctgg taaacccggc
      361 catcggtgaa aatcggcttg agaatcgact cgttgatccg ctcggcgatg gcgtctttcc
      421 cggccacccg ggcggtgttg cacaccgtcc cgagaacgtg cgagcacaac tccagcgtca
      481 ccgtcacaaa cccgccgtag tgggcgcaga gctgctcggc cggcgtcaga tcctcccagc
      541 ggttgtcggc gaccccgagg cagccgtagc cgtagatgtt gacgacagtg gtctccgcca
      601 cgctcaccgg cagcgtcagc cgccggcgcg aggggggcca gggctgcccc ttgcgcaccc
      661 agtcgaggaa gtcgccgttg gggaagacat agaaagagtc ggtcggcgtg cagcaatagg
      721 cccaccgccc gaagctgttc gatatctcga agctggttcc cccgagccag gtgaggcggt
      781 ggccgctgcg ctcgtaatcc ggctgaatag tcggcggcgc ctcggagggg ctggtcggcg
      841 cggccggagt cggcgcaagg gacgcggagc ggaccccctc ggcgacgaac gaattgacat
      901 actccgccac tacttcggtc agcaggctgt cgatctccgg atcgtccacc gtcagcgcca
      961 gcccatcggc ctccagcttg cggccgatca gggcggcgaa atcggcgaaa gccacctggg
     1021 cctcgagggc gtcgaggatg ctgtcggcct cagcggcgtc tgccatgcac acgaacggat
     1081 tgagaaacac aagcgccagc gccgtcgaac gggcgtcgag gagaacggga cccttcgctg
     1141 tcggatcggt gacgattccc atcagcacgg ggttgtggtc ggtatcgacc gccaggagcg
     1201 cggccgccga atgctcccac acgggcgcgg taaagcggcc ggaggagtcg acggcgccct
     1261 gaccgccgcc gcagagcacg gtcagatctt ccggcgccag cggcgattcg gggggaaggt
     1321 cgatcgcccc ggtgacgatc accggccggg gcggctcggg ctcggtgacc gtgtcgtcgt
     1381 tgccgcaata gagaaacagc accgcgctga agagcactgc ggggaacacc cacaggcggg
     1441 agtagcgaaa catggcggcc tcctctgagg cctcccgggc acagcccagt cccaaacgca
     1501 tggcggccta tctggcggca atataacaac ttcccgcact ctgtcaagga ataatggacg
     1561 aatcgcgcgc tgttgagcgg tccccgccga cgcgcctcag ggcccgccgg aaatccccgc
     1621 cgcgagcggt tgcgttttgg ggccgcaccc ctattatttc cgccgggact atggaatacg
     1681 ccgaccaccg cccgcccgcg gagcaccgca tccgccgcct tctggccgac cagttctacg
     1741 gagtcctctg cacgcgggga gatggccagc cgtacggttc gatggtggca ttcgcctgtt
     1801 ccgatgatct g
//
LOCUS       DAOOKS010000460         1807 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_83107_length_1807_cov_2.442922, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000460 DAOOKS010000000
VERSION     DAOOKS010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83107_length_1807_cov_2.442922"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1782
                     /locus_tag="PK112_07470"
     CDS             <1..1782
                     /locus_tag="PK112_07470"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HPC11922.1"
                     /translation="HREVALKIILAEYFNDAERLGRFQREAKMAARIAHPNVMGIYDI
                     GSAPDPDTGRDLEYIVMERVKGIPLGEYFQSAKPDMGGVIRVASRIAAGLAAAHKLNI
                     AHRDIKASNILVDDEGNPKILDFGLAKPVDPVQFDEGDRTDTVSQELTRAGKIVGTVS
                     YMSPEQARGDAVDIRTDIFSFGVLLYRMATGEFPFAGQSQVTTLAKILESKHEPPRLK
                     NQQIPTELERIIDKCLQKDPNDRYPSAADLTVDLRNLRRQFDSGVTESISGEYRVERT
                     VRVGGKRLAAIAAGLVVVGIVAVIVWRSGGERPGGAGPGGISPSVTTPGGNAVAVFDF
                     ENKTGDTSLNWLQTGLPEILMTDLAQTGSITVISRERVLDHLRTGSDLPDADEISDDV
                     RAEIEQARDEIREAVKSGSLDGVPAVKDALELARVSVAGLMRPHTYAEMAEAAGALGA
                     STVLSGSFFKLGERIRIDARLEDIGSGKIVLAEKVMGNDPFALVDSLTGKIVVIGRDR
                     DTATLELIENGELAASYAQNSYVEGFIALQWLHDYVNGDLKVIGDYIGGGVNPLPKTV
                     DSGSIVITKDNAAQFKEKYIWEVTAGK"
BASE COUNT          339 a          609 c          576 g          283 t
ORIGIN      
        1 caccgcgagg tggcgctcaa gatcattctg gcggagtact tcaatgacgc cgagcggctc
       61 gggcgtttcc agcgggaggc gaagatggcc gcccgcatcg cccaccccaa cgttatgggc
      121 atctacgaca tcgggagcgc gcccgacccc gacaccggcc gggacctcga gtacatcgtc
      181 atggagcgcg tgaaaggcat tcccctgggc gagtatttcc agagcgccaa gcccgacatg
      241 ggcggcgtga tccgggtcgc ctcgcgcatc gccgccggac tggccgccgc ccacaagctg
      301 aacatcgccc accgcgacat caaggcctcg aacatcctgg tcgacgacga ggggaacccc
      361 aagatcctcg actttggcct tgccaagccg gtcgaccccg tccagttcga cgagggcgac
      421 cgcaccgaca ccgtgtcgca ggaactgacg cgcgccggca agattgtcgg caccgtctcc
      481 tacatgtcgc ccgagcaggc ccgcggcgac gccgtcgaca tccgcaccga catcttctcc
      541 ttcggcgtcc tgctctaccg catggccacg ggcgaattcc cctttgccgg ccagtcgcag
      601 gtgacgacgc tggcgaaaat cctcgaatcg aaacacgagc ccccccggct gaaaaaccag
      661 cagattccga ccgaactcga acggatcatc gacaagtgcc tgcagaaaga tcctaatgac
      721 cggtacccgt cggccgcgga tttgacggtc gacctgcgca acctccgccg gcagttcgat
      781 tccggcgtga ccgagtcgat ctcgggcgag taccgggtgg agaggacggt ccgggtcggc
      841 gggaagcgtc tggctgcgat cgccgccggt cttgtcgtcg tcggaattgt cgcggtgatc
      901 gtctggcgct ccggcgggga gcggccgggg ggagccggcc ccggcggaat ctcgccctcg
      961 gtgacgaccc ccggcggcaa cgcggtcgcg gtcttcgatt tcgagaacaa aacgggggac
     1021 acctcgctca actggctcca gacgggcctg ccggagatcc tcatgaccga cctggcgcag
     1081 accgggtcga tcacggtcat cagccgggag cgcgtgctcg accacttgcg caccgggtcg
     1141 gacctgccgg atgccgacga gatttcggac gacgtgcggg ccgaaatcga gcaggcccgg
     1201 gacgagattc gcgaggcggt caaaagcggg tcgctcgacg gcgtgccggc ggtcaaggat
     1261 gcgctggaac tggcgcgggt gagcgtggcc ggactgatgc gcccgcacac ctacgccgag
     1321 atggccgagg cggccggggc gctcggcgcc tccacggtcc tctccgggtc cttcttcaag
     1381 ctcggcgagc gtatccgcat cgacgcgcgg ctcgaagaca tcggcagcgg gaagatcgtg
     1441 ctggcggaga aggtgatggg gaacgatccc ttcgccctcg tcgacagcct caccggcaag
     1501 atcgtcgtca tcggacgcga ccgcgacacc gccaccctcg agctcatcga aaacggcgaa
     1561 ctcgccgctt cctatgcgca gaactcctac gtggaaggct tcatcgccct ccagtggctg
     1621 catgactatg tcaacggcga cctcaaggtc atcggcgact atatcggtgg cggcgtgaac
     1681 ccgctgccca agacggttga ctccggctcc atcgtcatca ccaaggacaa tgccgcccag
     1741 ttcaaagaaa agtatatctg ggaagtcact gccggtaaat aatccagtcc ccatcgcttg
     1801 ctgtgtg
//
LOCUS       DAOOKS010000461         1806 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_83171_length_1806_cov_2.913764, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000461 DAOOKS010000000
VERSION     DAOOKS010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83171_length_1806_cov_2.913764"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="PK112_07475"
     CDS             complement(<1..589)
                     /locus_tag="PK112_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006475397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HPC11923.1"
                     /translation="MLDFFHAVTQYTFVLNALLTGILASVACGMVGSFVVARRITYIA
                     GGIAHSVLGGMGVARWLELVHGWPVKPLYGAVAAALAAALTIGVVSLKAREREDTVIG
                     AIWAIGMAVGILFIAATPGYNEDLMSYLFGNILMVGPVDLWLIGGLDLGVMAVTLLFF
                     NQLQAVCFDEEFARVRGLNVEAYYLLLLGLTALTVV"
     gene            complement(589..1347)
                     /locus_tag="PK112_07480"
     CDS             complement(589..1347)
                     /locus_tag="PK112_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPC11924.1"
                     /translation="MAERAAVVLEQVTFGYDSEPAVEEASFAIGEREYVWIVGPNGGG
                     KTTLVKLILGLLQPRRGTVRVFGAPPEEARHRIGYMPQHARLDPKFPVTVFEVALMGR
                     LGPRRWIGRYRRADRKAALRALDQVGLADMRNRNLAELSGGQQRRLLIARALAAEPEL
                     LLLDEPMANLDIRGENELTRLLQELDNRLTIIMVSHDPAFVAGSVKRVVCVKRRVHTH
                     PTATLDSGFMRELYGTEFRVVRHDVEQGSGEGAA"
     gene            complement(1350..>1806)
                     /locus_tag="PK112_07485"
     CDS             complement(1350..>1806)
                     /locus_tag="PK112_07485"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HPC11925.1"
                     /translation="TQVRTIAEALGRLDPGRREDYGARLAAFEHDLDSVHALIGEILA
                     PCAGRAVYVFHPSYGYFLDRYGLRQTAIEREGKEPAARDLADLIDAARADSVRALFVQ
                     PQFARKTAAAVAHAVGAELVTIDPLARDYLPNLVDMARRIAAGTAPEVK"
BASE COUNT          299 a          710 c          538 g          259 t
ORIGIN      
        1 tcaccaccgt cagtgccgtc agccccagca gcagcaggta gtaggcctcg acattcaggc
       61 cgcggacgcg ggcgaattct tcgtcgaaac agacagcctg caactggttg aaaaacagca
      121 gggtcacggc catcacgcca agatccagcc ccccgatgag ccacaaatca accgggccga
      181 ccatcagaat gttgccgaat aggtaactca tcaggtcctc attgtagccg ggcgtcgccg
      241 caatgaaaag gatccccacc gccatcccga tcgcccagat cgccccgatc accgtgtcct
      301 cccgctcgcg cgctttcagg ctcaccactc cgatcgtcag ggccgccgcc aaggccgccg
      361 ccaccgcccc gtagagcggc ttgaccggcc agccgtggac cagctccagc caccgcgcca
      421 cccccatgcc tcccagcacg ctgtgcgcga tcccgcccgc gatataggtg atccgtcgcg
      481 ccaccacgaa cgaccccacc atcccgcacg ccacgctcgc caggatcccg gtgaggagag
      541 cgttcagcac gaacgtatac tgcgtcaccg cgtggaaaaa atccagcatc aggcggcgcc
      601 ctccccggac ccctgttcga cgtcgtgccg caccacgcgg aactccgtgc cgtacagctc
      661 ccgcatgaac ccgctgtcga gcgtggcggt cgggtgcgtg tgcacgcgcc gcttcacgca
      721 cacgacgcgc ttgaccgacc cggccacaaa cgccgggtcg tgcgacacca tgatgatcgt
      781 gaggcggttg tccagctcct gcaggagccg ggtgagctcg ttttctcccc ggatgtcgag
      841 gttcgccatc ggctcgtcga gcaggagcag ttcgggttcg gccgcgagcg cgcgggcgat
      901 caggagccgc cgctgctgcc cccccgagag ctccgcgagg ttgcggttgc gcatgtccgc
      961 gagcccgacc tggtcgagcg cccgaagcgc cgccttgcgg tcggcccggc ggtaccgccc
     1021 gatccagcgc cgcggtccca gccgccccat gagggccacc tcgaaaaccg tcaccggaaa
     1081 tttcggatcc aaccgcgcgt gctggggcat gtacccgatc cggtgccgcg cctcctccgg
     1141 cggcgccccg aacacccgca ccgtcccgcg tcgcggctgc agcagcccca gaatcagctt
     1201 caccagcgtc gtctttccgc ccccgttcgg ccccacgatc cagacatact cccgctcccc
     1261 gatcgcgaaa ctggcctctt cgaccgccgg ctcgctatcg tagccgaacg tcacctgctc
     1321 cagcaccacc gccgcccgct ccgccatggt cacttcacct ccggggccgt cccggccgca
     1381 atccgccggg ccatgtcgac caggttgggg aggtagtcgc gcgcgagcgg atcgatcgtc
     1441 accagctccg cccccaccgc gtgcgccacc gccgccgccg tcttcctggc aaactgcggc
     1501 tggacgaaca gcgcccgcac gctgtcggcg cgcgccgcgt cgatcaggtc cgcgagatcc
     1561 cgggccgccg gctccttccc ctcccgctca atcgccgtct gccgcaggcc gtagcggtcg
     1621 aggaagtagc cgtaggaggg atgaaacacg tacacggccc ggccggcgca gggcgcgagg
     1681 atttcgccga tgagcgcgtg cacggagtcg agatcgtgct cgaacgcggc cagccgcgcc
     1741 ccgtagtcct cgcggcgccc cggatcgagg cggcccagag cctcggcgat tgtccgcacc
     1801 tgcgtc
//
LOCUS       DAOOKS010000462         1804 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_83297_length_1804_cov_3.586621, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000462 DAOOKS010000000
VERSION     DAOOKS010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83297_length_1804_cov_3.586621"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="PK112_07490"
     CDS             complement(<1..406)
                     /locus_tag="PK112_07490"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HPC11926.1"
                     /translation="MAASRGPVRKLAICGCGLIGGSLALDVQARGRGVEIAVWDRPGV
                     LGRIAADRRLRVRPEASFRHAVAGADVIVLAAPPTANGTLLRRLAARPEVAGALILDT
                     GSVKKPIADLAGSLTFPPGTQFLPSHPMAGREK"
     gene            complement(409..>1804)
                     /gene="argS"
                     /locus_tag="PK112_07495"
     CDS             complement(409..>1804)
                     /gene="argS"
                     /locus_tag="PK112_07495"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HPC11927.1"
                     /translation="VEYSSVNIAKPFGIGHLRTTILGNSLRRIFAKLGYRTVGMNYLG
                     DWGTQFGKMIVAYRQWGGGLDLSDNAVEKLVKLYVRFHQEAEGDEALNEQARKAFKDL
                     EDGEPSAVALWQQFRDLSIAEFMRVYRRLGIEFDVITGEAYLNKRMGPMIERLVRTGL
                     ASESQGALIVDLGDPQLPPLLLRKADGATLYSTRDLAGAFWRWESYRFHECLYVVATQ
                     QADHFRQVFRTVALVEEAENVPPEDRFAGRLKHIDFGWVRFGDANLSTRAGNIIFLED
                     VIDKAVALAREKIAEKNPGLVAIEDTAEMIGIGAVMFSQMSVRRQKDVNFDWDEVLSF
                     EGETGPYLQYTHARLCSLLRNYGREVSPSFDPVLLERNEEQRVIEALADFPEAVADAA
                     RAYDPFYVAAHLIRLAGAFNKLYQRKNEDGRIDKIISDDPALTEARIALVKAVQIVIN
                     EGLRLLGIRAPEEM"
BASE COUNT          311 a          590 c          606 g          297 t
ORIGIN      
        1 atttctcccg tccggccatc gggtgcgacg gcaaaaactg cgtccccggc ggaaacgtga
       61 gcgagccggc caggtcggcg atcggcttct tgaccgagcc ggtgtccagg atgagggcgc
      121 cggccacctc gggccgggcc gccaggcgcc ggagaagcgt cccgttcgca gtcgggggcg
      181 cggccaaaac aatgacgtcg gcgccggcga cagcgtggcg gaaactggcc tcggggcgaa
      241 cgcggagacg gcggtcggcg gcgatgcgcc cgaggacgcc cggccgatcc cagaccgcga
      301 tctccacgcc gcgcccccgc gcctgcacat ctagtgccag gctgccgccg atcaggccgc
      361 agccgcaaat ggcgagtttt ctcaccggcc cccgggacgc cgccacggct acatctcctc
      421 cggggcgcgg atccccagca ggcggaggcc ttcgtttatg acaatctgga cggccttgac
      481 cagcgcaatg cgggcctcgg tgagggccgg gtcgtcggaa atgatcttgt cgatgcggcc
      541 gtcctcgttc ttgcgctggt agagcttgtt gaacgccccg gccagccgga tcaaatgggc
      601 ggccacgtaa aacggatcgt aggcgcgcgc ggcgtcggcg accgcctccg gaaaatcggc
      661 gagcgcttca atcacccgct gctcctcgtt gcgctcaagc agaaccgggt cgaacgaggg
      721 cgacacctcg cgcccgtagt tgcgcagcaa cgagcagagc cgggcgtgcg tgtactggag
      781 atacggtccg gtctccccct cgaaactgag cacttcatcc cagtcgaagt tgacgtcttt
      841 ctgacgccgc accgacatct gggaaaacat cacggcgccg atgccgatca tttcggcggt
      901 gtcttcgatg gccacgaggc cgggattctt ctcggcgatt ttctcgcgcg ccagggcgac
      961 cgccttgtcg atgacatctt cgaggaagat gatgttgccg gcgcgggtcg agagattggc
     1021 gtcgccgaac cgcacccagc cgaaatcgat gtgcttgaga cggccggcga aacggtcctc
     1081 gggcgggaca ttctcggcct cctcgaccag cgcgaccgtc cggaagacct gccggaagtg
     1141 atcggcctgc tgggtggcca cgacatagag gcactcgtgg aaccggtagc tttcccagcg
     1201 ccagaaggcg ccggccagat cgcgggtgga gtagagcgtg gcgccgtcgg ctttgcgcaa
     1261 cagaaggggc ggcaactgcg ggtcgcctag atcgacaatc aacgccccct gcgactcgga
     1321 cgccagcccg gtgcggacca gccgctcgat catcggcccc atccgtttgt tcaggtacgc
     1381 ctcgccggtg atcacgtcga attcgatccc gaggcggcgg tagacgcgca tgaactcggc
     1441 gatggagagg tcgcggaact gctgccagag agcgaccgcg gacggttcac cgtcttcgag
     1501 atccttgaac gccttgcggg cctgctcgtt gagcgcctcg tccccctccg cctcctggtg
     1561 gaagcggacg tacagcttga ccagtttctc gaccgcgttg tcgctgaggt cgagaccgcc
     1621 gccccactgc cggtaggcga cgatcatctt gccaaactgc gtgccccagt cgccgaggta
     1681 attcatcccc accgtcctgt agccgagctt ggcgaagatc cggcggaggg agttgcccag
     1741 gatggtggtg cggaggtggc cgataccgaa gggtttggcg atgttgaccg aggaatactc
     1801 gacg
//
LOCUS       DAOOKS010000463         1802 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_83434_length_1802_cov_3.207785, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000463 DAOOKS010000000
VERSION     DAOOKS010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83434_length_1802_cov_3.207785"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="PK112_07500"
     CDS             <1..213
                     /locus_tag="PK112_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HPC11928.1"
                     /translation="WSDSPDALYDALTGKIDSRAVLSIELFKALESIGAYRRVIPASA
                     TEPSGLEDIYDMVQESFEGGEDLSDD"
     gene            244..417
                     /locus_tag="PK112_07505"
     CDS             244..417
                     /locus_tag="PK112_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015282923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11929.1"
                     /translation="MKTWVATCPDCGSADVVYEAGMMLGQKYRCLKCGYIGSFVLEKE
                     IEVSEEVSGEHDA"
     gene            complement(397..1569)
                     /locus_tag="PK112_07510"
     CDS             complement(397..1569)
                     /locus_tag="PK112_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome-activating nucleotidase"
                     /protein_id="HPC11930.1"
                     /translation="MVEKLELLERQNSELLEEVRRVEGEKRYVESELFRLQKEIKRMR
                     SELERLKSPPLIIGSIKDILIDGRVIVKSSTGPDFIVTTSEYVPSEDLIVGARVALNK
                     QTLAVMSVLPQSLDPIVTGAEIIEKPTITYDMIGGLDDQILEVREAVEDPLLRPELYK
                     RVGIEPPKGVLLVGPPGTGKTLIAKAVANHTNATFIRFVGSELVQKYIGEGARLVREL
                     FELAREKAPSIIFIDELDSVGAKRLDVATSGDREVQRTLMQLLAELDGFNPLDNIKII
                     GATNRPDILDDALLRPGRFDRIIEVPIPSYEARLEIFRIHTSKMSVEDKLNLEALATK
                     TELATGADIKSICTEAGMFAIRDNRDVVTMADFERAICKVLENETAKCTESGVMFA"
BASE COUNT          312 a          548 c          550 g          392 t
ORIGIN      
        1 tggtccgact ccccggacgc gctgtatgac gcgctcaccg gcaagatcga ctcccgggcg
       61 gtgctgagca tcgagctctt caaagcgctg gaatccatcg gcgcctaccg ccgggtgata
      121 cccgcctccg ccaccgagcc gtccggcctg gaggacatct acgacatggt gcaggagtcc
      181 ttcgagggcg gcgaggacct ctccgacgat tgatttaagt tcggcgaatc aggtcataac
      241 cgcatgaaga cctgggtggc cacctgcccc gattgcggtt ccgccgacgt ggtttacgag
      301 gcgggcatga tgctgggtca gaagtaccga tgcctaaagt gcggctacat cggttcgttc
      361 gttctggaaa aggaaataga ggtttcggaa gaggtttcag gcgaacatga cgcctgactc
      421 ggtgcacttg gccgtctcgt tctccagcac cttgcatatg gcccgttcga aatcggccat
      481 ggtcaccacg tcgcggttgt cgcggatggc gaacatgccc gcctcggtgc atatggactt
      541 gatgtcggcg ccggtggcca gctcggtctt ggtggccaac gcctccaagt tcagtttgtc
      601 ctccacgctc atcttgctgg tgtggatgcg gaagatctcc agacgggcct cgtagctcgg
      661 gatgggcacc tcgattatgc ggtcgaagcg tcccggccgc agcagcgcgt cgtccagtat
      721 gtccgggcgg ttggtagccc cgatgatctt gatgttgtcc agcgggttga agccgtccag
      781 ctcggccagc agctgcatga gggtgcgctg aacttcgcgg tctccggacg tcgctacgtc
      841 caaacgcttg gcgccgacgg agtcgagctc gtcgatgaat atgatgctgg gcgctttctc
      901 cctggccagc tcgaacagct cgcggaccag gcgggccccc tcaccgatgt acttctggac
      961 cagttcggaa ccgacgaagc ggatgaaggt ggcgttggtg tggttggcga cggccttcgc
     1021 aatgagcgtc ttaccggttc cgggcgggcc gaccagcagc acgcccttgg gcggctcgat
     1081 accgacgcgc ttgtacagct caggtcgcag cagggggtcc tcgaccgcct cgcgcacttc
     1141 caggatctga tcgtccagtc cgccgatcat gtcgtaggtg atggtcggct tctcgatgat
     1201 ctcggccccg gtgacgatgg ggtccagtga ctgcgggagg acggacatca cggccagggt
     1261 ctgcttgttg agcgccaccc gggcgcccac gatgaggtcc tccgacggca cgtactcgga
     1321 ggtggtgacg atgaagtccg gcccggtgct gcttttcacg atcacccggc cgtcgatgag
     1381 aatgtccttg atgctgccga tgatgagggg aggggacttc aaacgctcca gctcggagcg
     1441 catcctcttg atctccttct gcagccggaa gagctcgctc tccacatacc tcttctcacc
     1501 ctccaccctc ctgacctcct cgagcagctc gctgttctgg cgttccagaa gctccagctt
     1561 ctcgacgatc tcgttggaga tggattggtc aagctcttca tccaacaaat gtggcacctt
     1621 cgagcattga ttaaatcgcc ctctatttga gcatttgcca aaccctatca aacgatgatt
     1681 cttgtcatcc tggtcgcgcg gccggccatc actttatctc gattatcttg ggcgggcagt
     1741 agcccggttc gcagatgtcc ttcttctcca actgggcggg gtacagcacc ttggtgaaga
     1801 cc
//
LOCUS       DAOOKS010000464         1794 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_84014_length_1794_cov_3.392754, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000464 DAOOKS010000000
VERSION     DAOOKS010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84014_length_1794_cov_3.392754"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            523..>1794
                     /locus_tag="PK112_07515"
     CDS             523..>1794
                     /locus_tag="PK112_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HPC11931.1"
                     /translation="MFLKKFLDQLKAHRQTWDQTAEKYRAQKSMPRFFTVSGVDINDL
                     YTPDSIAGADREDYFWRNLGFPGEYPYTRGVHHTLYRTKLWTMRQFAGMGTPRQTNER
                     FHYILANGGTGLSTAFDLPTLMGYDSDHPRSLGEVGKCGVAVDTLADMEIIFDRIDLG
                     RVSTSMTINSPASMILAMYLAVAEKQGVPFAAVTGTLQNDILKEYIAQKEFIFPPRPS
                     LRLITDMIAYCTRHVSKFNTISISGYHIREAGSTAVQELAFTLADGFQYVDSCLEAGM
                     GVDEFAPRLSFFFNAHSDFFEEIAKYRAARRIWARHLRERYRAQDERSWKLRFHTQTA
                     GVSLTAQQPENNIARVAIQGLSAVLGGTQSLHTNSMDETLALPSEKAARIALRTQQII
                     AYETGVANTIDPLAGSYFVEALTDQMEAGAEA"
BASE COUNT          321 a          649 c          505 g          319 t
ORIGIN      
        1 tgttcgggtg gaacgcccgc gccttgtaga acatcgtgag cgggcgcgag ggatacgggg
       61 tgaatgcatc cacgagcaca ccttggcgac ggaactcgct ccacaggtat ctcgaggacc
      121 ccgaaaatgt cctgatgtcc cagggatcgc cgtagtcaat ccccagcatc ctgacacaat
      181 cgcagttcgg ccttgcgatg agtcacctcc ccttgcttca agggtatgtc gggcgtcgtg
      241 ctgtcggccg ccggcgaccc ggccgccggg cgatctctgg atcgcagaga tatacgccct
      301 atagttccag tatacaccgg tcggcccgac acggcaagga ctttcgcgcg gtgcgctcct
      361 ccgatgacgg gcggtttgcg agacccggcc gctaacccca tgagaatctt ttgctttccc
      421 ggcgccgccg ggggcggatt ttcccccgcg ccgcttgcgt ttccccgccc gaccgcttat
      481 cttcgagtct tgaacactgt aacctgaagg agtgagccgg ccatgttcct gaagaaattt
      541 ctcgaccagc tcaaggccca ccgtcagacc tgggaccaga cggccgagaa gtaccgcgcc
      601 cagaaatcca tgccccgttt cttcaccgtc tccggcgtcg acatcaacga cctctacacc
      661 cccgattcca tcgcaggcgc cgaccgcgag gattacttct ggcgcaacct cggcttcccc
      721 ggcgagtatc cctacacccg cggcgtgcac cacaccctgt accggaccaa gctctggacc
      781 atgcgccagt tcgccggcat gggcaccccc aggcagacca acgagcgttt ccactacatc
      841 ctcgccaacg gcggcaccgg cctctcgacc gcctttgacc tgcccaccct gatggggtat
      901 gattccgacc acccccgttc gctcggcgag gtggggaagt gcggggtggc cgtcgacacg
      961 ctggccgata tggagatcat cttcgaccgc atcgacctcg gccgggtctc gacctccatg
     1021 accatcaact cgccggcgtc gatgatcctc gcgatgtacc tcgcggtggc cgagaagcag
     1081 ggcgttccgt tcgccgcggt caccggcacg ctccagaacg acatcctcaa ggagtatatc
     1141 gcgcagaagg aattcatctt cccgccgcgc ccgtcgctcc gcctcataac cgacatgatc
     1201 gcctactgca cgcggcacgt ttcgaaattc aacaccattt cgatctccgg ttaccacatt
     1261 cgcgaggccg gctcgaccgc cgtgcaggag ctggcgttca ctctcgccga cggtttccag
     1321 tacgtcgatt cgtgcctcga ggcggggatg ggcgtcgatg agtttgcgcc gcggctgtcg
     1381 ttcttcttca acgcccacag cgacttcttc gaggagatcg ccaaataccg cgccgcccgc
     1441 cgcatctggg cgcgccacct gcgcgagcgc taccgggcgc aggatgaacg ctcgtggaaa
     1501 ctccgcttcc acacccagac cgcgggcgtc tccctgaccg cccagcagcc cgagaacaac
     1561 atcgcccgcg tcgccatcca gggactctcg gccgtcctcg gcggcacgca gtcgctccat
     1621 acgaattcga tggatgagac gctcgcgctg ccctcggaga aggccgcccg gatcgccctg
     1681 cgcacccagc agatcatcgc ctacgaaacc ggcgtcgcca acaccatcga cccgctcgcc
     1741 ggctcgtact tcgtcgaggc cctcaccgac cagatggagg ccggggccga ggcc
//
LOCUS       DAOOKS010000465         1794 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_84021_length_1794_cov_3.211041, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000465 DAOOKS010000000
VERSION     DAOOKS010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84021_length_1794_cov_3.211041"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1794
                     /locus_tag="PK112_07520"
     CDS             <1..>1794
                     /locus_tag="PK112_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11932.1"
                     /translation="NQALSFYPVLRIGRYFATAAAAALLVVALLPGMFRYSFLVFSNP
                     TTEIAPPLAYKVVPFPGTTEWVKYRDIEIGASIFGERLPESAVIHHRLVDGNWQETEI
                     DLRKVRRAPMVNGDSVRIATTLRQINRSFDYYVEAGRVATPVQQVSVVDRPRVNGLKL
                     SIFYPDYTGLAPTVIDENNGSFSAVVGSRVNLAVETNLPVARAELVFDDSSRTPLEVD
                     GRQAETALRVDKSLGYHVRLIDGLGEENPDPIQYHITAVPDEYPSIDVLFPGVNVNLS
                     DEMVLPLKVRIFDDYGFTSLVLKYTVVAHGQASEENVAVLHFSDRIKTEGEIAFNWDM
                     DKLNMYPGDYTIYRFEVADNDVNSGPKVTASRQYVARIPSLEEIIAEAEGESSRRVDN
                     TRQLLQQGEDLSERFKNMARKLKAQNRDEMKAEWQHQKEMEALAAQNQELIENIEKMA
                     EEMDKSVEKLADNAQMSREIVEKLQQIQKLFEEVATPEMKEAQKKLMEALQKMDRNEI
                     QKAMEQFQLSQEEMLQRLERTLALLKQMQVEQKMQAMMRQAERLLEQQNQVNQATDSS
                     AQSALPPLAERETDLKKDLESLKKETADLRKL"
BASE COUNT          390 a          549 c          592 g          263 t
ORIGIN      
        1 caaccaggcg ctctcgttct acccggtgct gcgcatcggg cggtatttcg cgaccgcggc
       61 cgccgcggcc ctcctggtcg tggccctcct gccggggatg ttccgctact cgttcctcgt
      121 gttctccaac ccgacgaccg aaatcgcccc tcccctggcc tacaaggtgg tgccgttccc
      181 cgggacgacc gagtgggtga agtaccggga catcgaaatc ggggcctcca ttttcggtga
      241 acggctcccg gaaagcgcgg tcattcacca ccggctggtc gacgggaact ggcaggagac
      301 ggagatcgac ttgcggaagg tgcggcgggc gccgatggtc aacggcgact cggtgcggat
      361 tgcgaccact ctccgccaga tcaaccggtc gttcgactac tatgtcgagg cgggccgggt
      421 cgccaccccg gtccagcagg tgagcgtggt cgaccgcccg agggtcaacg gcctcaagct
      481 gtcgatcttc taccccgact acaccgggct ggccccgact gtgatcgacg agaacaacgg
      541 ctcgttctcg gccgtggtcg ggtcgcgggt gaacctggcg gtcgagacca acctcccggt
      601 cgcgcgggcc gagctggtgt ttgacgattc cagccggacg ccgctggagg tcgacgggcg
      661 gcaggcggaa acggcgctcc gcgtggacaa gtcgctcggc taccatgtcc gcctgatcga
      721 cggcctgggc gaggaaaacc ccgacccgat ccagtaccac atcacggccg tgcccgacga
      781 atacccctcg atcgacgtcc tcttccccgg cgtcaacgtc aacctgagcg acgaaatggt
      841 gctgccgctg aaggtgcgga tattcgacga ctacgggttt acctcgctgg tgctgaaata
      901 cacggtggtg gcgcacggac aggcgagcga ggagaacgtg gccgtgctcc atttctccga
      961 ccggatcaag accgaagggg agatcgcctt caactgggac atggacaagc tcaacatgta
     1021 cccgggggat tacacgatct atcgcttcga ggtggccgac aacgacgtga actcggggcc
     1081 gaaagtgacg gcgtcgcggc agtacgtggc gcggatcccc tcgctggagg aaatcatcgc
     1141 cgaggcggag ggggaaagct cgcggcgcgt ggacaacacc cggcaactgc tccagcaggg
     1201 ggaggacctg tcggagcggt tcaagaacat ggcgcgcaag ctcaaggcgc agaaccgcga
     1261 cgagatgaag gcggagtggc agcaccagaa ggagatggag gcgctcgccg cccagaacca
     1321 ggagctcatc gagaatatcg agaaaatggc cgaggagatg gacaagtcgg tcgagaagct
     1381 cgccgacaac gcccagatga gccgcgagat tgtcgaaaaa ctccagcaga tccagaagct
     1441 gttcgaggaa gtcgccacgc cggaaatgaa ggaggcccag aagaagctga tggaggcgct
     1501 ccagaaaatg gaccgcaacg agatccagaa agccatggag cagttccagc tctcccagga
     1561 ggaaatgctc cagcggctgg agcggacgct ggccctcttg aaacagatgc aggtcgagca
     1621 gaagatgcag gcaatgatgc ggcaggcgga gcggctgctc gaacagcaga accaggtcaa
     1681 ccaggcgacc gacagcagcg cccagagcgc gctgccgccc ttggccgaac gcgaaacgga
     1741 cctgaaaaaa gatctcgagt cgctcaaaaa ggagaccgcc gaccttcgca agct
//
LOCUS       DAOOKS010000466         1790 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_84286_length_1790_cov_4.076081, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000466 DAOOKS010000000
VERSION     DAOOKS010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84286_length_1790_cov_4.076081"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="PK112_07525"
     CDS             <1..324
                     /locus_tag="PK112_07525"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005009081.1"
                     /note="catalyzes a two-step reaction, first charging a
                     glutamine molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HPC11933.1"
                     /translation="WVSAAHAFEGEVRWYENLFSNEFPEEIAPEEAAAGKDFTAHINP
                     HSLRILTGCRLEPMLKEARPGDRFQFLRHGYFCVDNKFSAPGRPIFNRTVGLRDTWAK
                     LAAKD"
     gene            573..>1790
                     /locus_tag="PK112_07530"
     CDS             573..>1790
                     /locus_tag="PK112_07530"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013011098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HPC11934.1"
                     /translation="MADSRIEALRRKRGQMTAGGGPQAVDKQHERGKLTARERIDLLF
                     EPGTFRESLLFIQHRQTAFGMAGKEFPGEGVVCGHGIIDGRPVYVASQDFTVGGGSVG
                     EMTGRKISEAMDAALKTGDPLVMINDSGGARIQEGVDSLQGYGHIFYRNVLLSGVVPQ
                     ISIIAGPCAGGAAYSPALTDFIIQVRHQGQLYITGPLVIKQVTGEEITAEQLGGAESH
                     AYYSGVVHFIAENDEHALALARRLLSYLPSNNTEDPPFDAALHEEAIGPDPAMNEIVP
                     EDPREPYDMREVIAGILDQGDFLEVMEHFAPNILIGFGRLAGYSIGVVANQPVQKAGV
                     LDIDSSDKAARFIRFCNAFNIPILTLVDVPGFMPGVQQEFSGIIRHGAKMLFAYAAAT
                     VPKMTVVIRKAYGG"
BASE COUNT          334 a          545 c          620 g          291 t
ORIGIN      
        1 tgggtgtcgg ccgcgcacgc gttcgaaggc gaggtgcggt ggtacgagaa cctgttctcg
       61 aacgagtttc cggaggagat tgcgccggag gaggcggccg ccgggaagga tttcaccgcc
      121 cacatcaacc cgcattcgct gcgcatcctg accggctgca gactcgaacc gatgctgaaa
      181 gaggcccgtc cgggcgaccg cttccagttt ctgcgccacg gctatttctg cgtggacaac
      241 aagttctccg cgcccgggag gccgattttc aacaggaccg tgggcctccg ggacacgtgg
      301 gcgaaactcg ccgcgaaaga ctagccgcgc gcacgcgaca tcggacattc gggccgccgt
      361 cgggtatccg ccggcggtct ccttttttgc gggaaagggg tgacagccgg cggcaggaaa
      421 agcgcggtcg accggcctcc gccgcccccc ggcgggcgcc ctgtgcgcgg ccgcgccaaa
      481 agcggagcgg gggcaaacta tcgcttgcat tttagaggaa agcacttaca ctaccggggc
      541 gaagtatgtg aaatttgtaa caaggattgg acatggctga ttcacgcatc gaggccctgc
      601 gccggaaacg cggccaaatg accgcggggg gcggtcccca ggcagtcgac aaacagcacg
      661 agcgcggcaa gctgaccgcc cgcgagcgca tcgacctgtt gtttgagcca gggacattcc
      721 gcgagtcgct tctgttcatc cagcaccggc agacggcgtt cgggatggcg gggaaggaat
      781 ttccgggcga aggggtggtg tgcgggcacg ggatcatcga cggccgcccg gtgtacgtcg
      841 cgagccagga tttcacggtc gggggcgggt cggtgggtga aatgaccgga cggaagatca
      901 gcgaagccat ggacgcggcg ctcaaaaccg gggatcccct ggtcatgatc aacgactccg
      961 gcggcgcgcg gattcaggag ggggtggact cgctccaggg atacgggcac attttctacc
     1021 gcaacgtgct cttgtccggg gtggtgccgc agatcagcat aatcgccggt ccgtgcgcgg
     1081 gcggcgcggc ctactccccc gcgctgaccg attttatcat ccaggtgcgc caccagggac
     1141 agctctatat caccggcccg ctcgtgatca agcaggtgac gggcgaggag atcacggccg
     1201 aacagctcgg cggcgcggag agccacgcct actactcggg ggtggtgcat ttcattgcgg
     1261 aaaacgacga acacgcgctc gccctcgcgc ggcggctgct gtcctacctg ccgagcaaca
     1321 acaccgagga cccgccgttc gacgcggcgc tgcacgagga ggcgatcggc cccgatccgg
     1381 cgatgaacga aatcgtgccg gaggatcccc gggaaccgta cgacatgcgg gaggtgatcg
     1441 ccggaatcct cgaccagggg gacttcctcg aagtgatgga gcactttgcc ccgaacattc
     1501 tgatcgggtt cgggcggctg gcggggtatt cgatcggggt ggtggcgaac cagcccgtgc
     1561 agaaagcggg ggtgctcgat atcgattcct cggacaaggc ggcgcggttc atccggttct
     1621 gcaacgcctt caatatcccc attctcacgc tcgtggacgt gccggggttc atgccggggg
     1681 tgcagcagga attcagcggg attatccgcc acggcgcgaa gatgctcttc gcctacgccg
     1741 ccgcgaccgt gccgaagatg acagtggtga tccgcaaggc ctacggcggc
//
LOCUS       DAOOKS010000467         1789 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_84379_length_1789_cov_3.044983, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000467 DAOOKS010000000
VERSION     DAOOKS010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84379_length_1789_cov_3.044983"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            508..1317
                     /locus_tag="PK112_07535"
     CDS             508..1317
                     /locus_tag="PK112_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11935.1"
                     /translation="MLTPKRYVCLVRPQAKYARIDRYQAVLSHDPARAGRWSDSLNIV
                     AAGIDSTVRAAFDQNAWLDLPKESFFAPVFLELKRNAVVDRSPLALNSDHYLVLPETF
                     PTRTRFAITYHIPYDVEPNRYSGLCWLDKNTNRWVWLENSFDDDRDVLTATAGGGGSF
                     GAVFDYETPRISNLSLREGGVYQNRRPAVNFVLEDTLSGIADDRNIVIELDGEWLIPE
                     YEPETGRVHSRPLAPLAPGEHYLSIEATDRVGNKTGKYLRFRVAKAGGGDR"
     gene            1381..>1789
                     /locus_tag="PK112_07540"
     CDS             1381..>1789
                     /locus_tag="PK112_07540"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPC11936.1"
                     /translation="MTVGLIAANTLVFLYSIMGSPRAFQLFIVQYGFIPYELVHFTEL
                     TPQVAAPVWLTMFSSMFMHGGWMHLLGNMLFLWIYGNNIEDYFGPVRFILFYLLSGLS
                     AIFLFTLFGPNSQTPLVGASGAISGVMGAYLVLH"
BASE COUNT          303 a          573 c          574 g          339 t
ORIGIN      
        1 cgcgatcaca gcggaggatg ctttcgggaa caggtcggaa ctccggttca gggtcgtgct
       61 cgggccgggg gagttcctct acgggctcga ttcgaccgtg cgggtggcag cggacacgac
      121 cgatttctat ttcacggcta gcgaagcact ggccacggtg gcggtcgatt cgatcgtgct
      181 ctatgtcgga cggggcgacc gctggggctg gacgcccgac gggacgatcg agcggctcga
      241 cggcgggcgg ctgaaagtgc gcgccggcgg ctacaaaacc cgcgtcacgc cgttgcgcct
      301 ctacctcttc actgaagagg ggggcgtgat cgccgacggc gtgttcaacg gactgcgcga
      361 tgacgacacc gggggaaagt tcgaaacctc ccacgagctg accgaggacg gcctgctcat
      421 caagatcacc tcctggaatc ctcggcctac ctctcgcgcg tcgatctctt ctaccggggg
      481 aaactgctgg ggacggagta cgggcggatg ctcacgccca agcggtatgt gtgcctggtg
      541 cggccgcagg ccaagtacgc gcggatcgac cggtaccagg cggtcctctc gcacgacccg
      601 gcgcgggcgg ggcggtggtc cgactccctg aatatcgtcg cggcggggat cgattcgacg
      661 gtccgggcgg ccttcgacca aaacgcctgg ctcgatctgc ccaaagagtc cttcttcgcc
      721 cctgtgttcc tcgaactcaa gagaaacgcc gtcgtcgacc ggtctcccct ggccctcaat
      781 tccgaccact acctcgtgct gccggagacc ttcccgaccc gcacgcgctt cgccatcacg
      841 taccacatac cgtacgacgt ggagcccaac cggtacagcg gtctctgctg gctggacaag
      901 aacaccaatc ggtgggtgtg gctggagaat tcattcgacg acgaccgcga cgtgctgacg
      961 gcgacggcgg ggggaggcgg gtcgttcggg gcggtgtttg attacgaaac cccgcggatc
     1021 tcgaatctgt cgctgcgcga aggcggggtg taccagaacc gccggccggc cgtcaatttc
     1081 gtcctcgagg acaccctgtc gggcattgcc gacgatcgga acattgtcat cgagctcgac
     1141 ggcgagtggc tgatcccgga atacgagccc gagaccgggc gcgtgcacag ccggccgctg
     1201 gcgccgctgg cgccgggcga acactacctc tcgattgagg ccaccgaccg cgtgggcaac
     1261 aagaccggca agtaccttcg ctttcgcgtc gcgaaagcgg ggggcggcga ccggtgacgg
     1321 ctgaggcgaa aattgtttat tcccatacgc gatgatactc cgactatccg cagaccgtac
     1381 gtgacggtgg gcctgatcgc cgccaacacg ctcgtgtttc tgtactccat catgggcagc
     1441 ccccgggctt tccagctctt cattgtccag tacggtttca tcccctacga gctcgtgcat
     1501 ttcacggaac tcacgccgca ggtcgccgcg ccggtgtggc tcaccatgtt ctcgtcgatg
     1561 ttcatgcacg gagggtggat gcacctcctg ggcaacatgc tgttcctgtg gatctacgga
     1621 aacaacatcg aggactactt cggccctgtc cgattcatcc tgttctacct gttgtcgggg
     1681 ctgtcggcga tttttctgtt cacgctcttc ggccccaatt cgcagacgcc cctggtgggc
     1741 gcctcgggcg cgatctcggg cgtgatggga gcctacctgg tgctccacc
//
LOCUS       DAOOKS010000468         1783 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_84804_length_1783_cov_2.787616, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000468 DAOOKS010000000
VERSION     DAOOKS010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84804_length_1783_cov_2.787616"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1783
                     /gene="gatE"
                     /locus_tag="PK112_07545"
     CDS             <1..>1783
                     /gene="gatE"
                     /locus_tag="PK112_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatE"
                     /protein_id="HPC11937.1"
                     /translation="CRCPAGIYHHRDDFDAEVIRHMRPTLSEMGEYDGTALMEKRTKK
                     NIIYRIKDRTACTYEIDDTPPFKIDREALEIAIEVALLLKTNIVGELHITRKQYLDGS
                     IPTGFQRTAIVGIEGEIPLAARKVRIIQLSVEEDSCREVSDVAHDRTYTTDRLGMPLI
                     ETVTYPDMLTPDEAAEAAQYLRYLARSTGHVRTGIGAAREDVNVSITGGTRVEIKGVA
                     KIKWIPELTHNEAFRQRALLEIARILRERVGDPAAWRISQADLPFAVFEQPYAVWASA
                     RQRGGRLAVINLPDFKGILSFFTQPGQMFAHELEGRVKVIACLERPNIVHSEQPDLVL
                     SAADWAAVYERLQPGPGDAQVLVWGPPADVATAIETIEERCRMAFDGVPNETRKALPD
                     GTTIFERVLPGPDRMYPDTDSAPLSVSEELIERLRRRLPVEVAERMRQLSTWQVPADA
                     HRYLLARNLTPLIERIASDWSYEPRWVGKVLGHGLKNIERENISEGAPLDYERLYGLF
                     AFVKEQGLDREIIKAMLPAAYRNPQAGFAEILERTGFRRRSEGDLLGPCGELIDGGVD
                     RTRDAYRHPHADARAERDQHDRRDENRP"
BASE COUNT          351 a          512 c          641 g          279 t
ORIGIN      
        1 tctgccgctg tccggcgggc atctaccacc accgggatga ctttgacgcc gaggtcatcc
       61 ggcacatgcg cccgacgctg tcggagatgg gggagtacga cggcacggcg ctgatggaaa
      121 agcggacgaa gaagaacatc atttaccgga tcaaggaccg gaccgcctgc acctacgaaa
      181 ttgacgacac gccgccgttt aagatcgacc gggaggcact ggaaatcgcg atcgaggttg
      241 cgcttctgct gaaaaccaac attgtcggcg agcttcacat aacgcgaaag cagtacctgg
      301 acgggtcgat cccgacgggt tttcagcgca cggcgattgt cggcatcgag ggggaaattc
      361 ccctggcggc caggaaggtg cggatcatcc agttaagcgt cgaggaggac agctgtcggg
      421 aagtgtcgga cgtcgcccac gaccggacct acacgaccga ccggctgggg atgccgctga
      481 tcgaaacggt gacatatccg gacatgctga cgcccgacga agcggcggaa gcggcccaat
      541 acctgcggta tctcgcgcgg agcacgggtc acgtgcggac cgggatcggg gcggcccgag
      601 aggatgtgaa tgtgagcatc accgggggaa cgcgggtgga aatcaagggg gtggcgaaga
      661 tcaagtggat accggaattg acccacaacg aggcgtttcg ccagcgggcg ctgctggaga
      721 tcgcccggat tctgcgggag cgggtgggtg atccggccgc ctggcggatc agccaggcgg
      781 acctgccctt cgccgtcttc gagcagccct acgcggtctg ggcgagcgcg cgccaacgcg
      841 gggggcggct ggcggtcatc aacctgccgg atttcaaggg gatactctcg ttcttcaccc
      901 agccggggca aatgtttgcg cacgaactgg aggggcgggt gaaagtgatt gcttgcctgg
      961 agcgcccgaa tattgtgcac agcgagcagc ccgacctggt gctctcggcg gccgactggg
     1021 cggccgttta cgaacggctg caaccggggc cgggggatgc ccaggtcctg gtgtgggggc
     1081 cgccggccga cgtagccacg gcaatcgaga cgatcgaaga acggtgccgg atggcttttg
     1141 acggcgtgcc gaacgaaacg cggaaagcgc tgccggacgg gacgacgatt tttgagcggg
     1201 tcctgccggg accggaccgg atgtacccgg acaccgactc ggcgccgctg tcggtgtcgg
     1261 aggagctgat cgagagactg cggaggcgcc tgccggtcga ggtggccgag cggatgcggc
     1321 agttgagcac ctggcaggtg ccggcggacg cacaccggta tctgctggcc cgcaatctga
     1381 caccgctcat cgagcggatc gcgtccgatt ggagctacga gccgcgctgg gtcggcaaag
     1441 tgctcggcca cggactgaaa aacatcgaac gcgagaatat ctcggaaggg gcgccgctgg
     1501 attatgaacg gctgtacggg ctgttcgctt tcgtgaagga gcaggggctg gaccgggaga
     1561 tcatcaaggc aatgctcccg gcggcctacc gcaacccgca ggccggtttt gcggagattc
     1621 tggagcgcac gggattccgc cggcgaagcg agggcgacct gctcggaccc tgcggcgagt
     1681 tgatcgatgg cggtgttgat cggacgcgag atgcttatcg ccatccacac gccgatgccc
     1741 gtgccgagcg tgaccagcac gatcgacgag acgagaatcg tcc
//
LOCUS       DAOOKS010000469         1779 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_85086_length_1779_cov_2.656613, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000469 DAOOKS010000000
VERSION     DAOOKS010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85086_length_1779_cov_2.656613"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="PK112_07550"
     CDS             <1..502
                     /locus_tag="PK112_07550"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HPC11938.1"
                     /translation="IGKIGIPDAVLIKPGELTPPEREIIKSHVSRGAGIIEVVTWLAD
                     AIDVIRYHHERWDGTGYLAGLCGVEIPLTARIFAVADVFDALTSRRPYKLSYNFDQAR
                     DMVVAGAGSQFDPDVVRVFAARAEDLYAEGHRAATRRLEEVIRRFIREYFAVEWDRAV
                     QMEPSM"
     gene            654..1562
                     /locus_tag="PK112_07555"
     CDS             654..1562
                     /locus_tag="PK112_07555"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPC11939.1"
                     /translation="MLHSIWPRTGAAAVLLVVLAFACRGGGEGGPADASAALRVDSAL
                     STDSVPIYFEVHGSGDTALVFVHGWGIDGSFWEKQIPAFSDSYTVVTLDLAGHGRSGK
                     NRAAWTPHAYGADVVAVLDAAGIAHAFLVGHSMGGVIIAEAARLAPDRVIALIGADTY
                     QDFRERWTPDQAEQFLAPFRRNYDSTVAWFASVMLPENVDTAIAYRIIRSFQADDPAV
                     GLATLGNLLVYDALPVLREAGKPIRAVSSDSYPTNLAANREIEPTFDVRLMSGVGHFL
                     FLERPEEFNRLLGQTLAELRAGGAAP"
     gene            complement(1633..1779)
                     /locus_tag="PK112_07560"
                     /pseudo
     CDS             complement(1633..1779)
                     /locus_tag="PK112_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017802403.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
BASE COUNT          271 a          641 c          562 g          305 t
ORIGIN      
        1 catcggcaag atcggcattc ccgacgccgt cctcatcaag cccggcgaac tcacgccccc
       61 ggaacgcgag attatcaagt cccacgtgtc ccggggcgcc ggcatcatcg aagtggtcac
      121 ctggcttgcc gatgcgattg acgtcatccg ctaccaccac gagcgttggg acggcaccgg
      181 ctacctggcc gggctctgcg gggtcgagat tcccctcacc gcccgcatat tcgccgtggc
      241 cgacgtcttc gatgcgctca cgagccgaag gccctacaag ctgtcgtaca atttcgatca
      301 ggcccgcgat atggtcgtcg ccggcgccgg cagccagttc gacccggacg tcgtccgcgt
      361 cttcgccgcc cgtgccgagg atctctacgc cgagggacac cgggcggcca cccggcggct
      421 cgaagaggtc atccgccggt tcatccggga gtatttcgcc gtcgaatggg accgcgcggt
      481 gcagatggaa cccagcatgt gatcggccgc caacgacaaa ataccccttg accccctttc
      541 cggactccgt agatttccgc gaacgcggtc cggcggcccg ccgtgatcgt gcgccgcgcc
      601 ggcggtgcgt ccggccgccc gcgccgcgcc ggccaccgga agagaggaaa catatgctgc
      661 attcgatttg gcctcgcacc ggggctgctg cagtgctgct cgtggtgctc gcgttcgcct
      721 gccggggcgg gggagaaggg gggccggcgg atgcctccgc cgcgctccgc gtcgattccg
      781 ccctgtccac cgacagtgtc ccgatctact tcgaggtcca cggcagcggc gacaccgcgc
      841 tggtctttgt ccatggctgg ggcattgacg gcagcttctg ggagaaacag attccggctt
      901 tcagcgactc ctacacggtc gtcaccctcg atctggccgg ccacggccgt tcggggaaga
      961 accgggccgc gtggactccg catgcgtatg gcgccgatgt cgtcgcggtc cttgacgcgg
     1021 ccgggatcgc gcacgcattc ctggtcggcc acagcatggg cggcgtcatc atcgccgagg
     1081 ccgcccgtct tgccccggac cgggtcatcg ccctgatcgg cgccgacacc taccaggatt
     1141 ttcgcgaacg ctggaccccg gaccaggccg agcagttcct cgcgccgttc cgccggaact
     1201 acgactccac cgtcgcctgg ttcgccagcg tcatgctgcc ggagaatgtc gacaccgcca
     1261 ttgcctaccg catcatccgc tctttccagg ccgatgatcc cgcggtcggc ctggccactc
     1321 tcgggaacct cctggtctac gacgccctcc cggtgctgcg ggaagccggc aaaccgattc
     1381 gcgcggtcag cagcgactcg tacccgacca acctcgcggc caaccgcgaa atcgagccga
     1441 ctttcgacgt cagactcatg agcggcgtcg ggcacttcct cttccttgag cgccccgagg
     1501 aattcaatcg tctcctgggc cagaccctgg cggagctccg cgccggcggc gccgcgccgt
     1561 aagcctggtg cacagcgatt tgcggggcgg gcgtcgaaag cgtccccgct cggtctgctc
     1621 ttggggagag ggctattcgt cgcggaagat tttgacgacc ggatcgcggc ccggataccg
     1681 atacccgcgg tacatgccgg ggctgttgaa cggcatggcg atcgtccggc ccgatcgacc
     1741 gcgaccaccc cgccctggcc tcccagcgcg gtgagcttt
//
LOCUS       DAOOKS010000470         1776 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_85305_length_1776_cov_2.592098, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000470 DAOOKS010000000
VERSION     DAOOKS010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85305_length_1776_cov_2.592098"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            221..796
                     /locus_tag="PK112_07565"
     CDS             221..796
                     /locus_tag="PK112_07565"
                     /inference="COORDINATES: protein motif:HMM:NF024635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPC11940.1"
                     /translation="MDKADKAPTAPVLALVGMPGSGKSTAAEMLAARGWATIRFGQVT
                     IDEIAARGLTVDERNERAIREELRRVHGPDAYARLAMPKILAARETGRVLIDGLYSWS
                     EYKYLHEALASRMLVLAITSARRIRYARLAGRSERPLTAEEARRRDFAEVENLEKGGP
                     IAMADYTIVNDAALDDLRREVDRAIAFLFPS"
     gene            801..1136
                     /locus_tag="PK112_07570"
     CDS             801..1136
                     /locus_tag="PK112_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11941.1"
                     /translation="MDERAIKKLLAQERQRWFGWGHEDWREMIEATPDDEDGELFFYL
                     APFGGEAFPVEVVCFPDPDGAMEISLIVTIEENGREAEAAWMVYADGEKEMLVETTDR
                     REGDEEGDE"
     gene            1129..1437
                     /locus_tag="PK112_07575"
     CDS             1129..1437
                     /locus_tag="PK112_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11942.1"
                     /translation="MSKGPQTGRLAPPDINRLLQEERLRWARRPYEFLRTVPSHPEGP
                     LAYYRFRSGRTFFVRLAVVSATEARVELRVTAEEQAGCLGFGRRPAGKELTFTVLRPV
                     "
     gene            complement(1458..>1776)
                     /locus_tag="PK112_07580"
     CDS             complement(1458..>1776)
                     /locus_tag="PK112_07580"
                     /inference="COORDINATES: protein motif:HMM:NF014171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HPC11943.1"
                     /translation="IRSDSYEMLRTVSHPAPRMADLPAGISDVEITSVRVVPDSPFAG
                     RNLAQLNLRARCGVNILAIRRGEQVTANPGADAVVEAEDVLYMLGSPAACLAAEQLLR
                     PEE"
BASE COUNT          281 a          528 c          705 g          262 t
ORIGIN      
        1 gcggcagccg accgtctgga ggcggttgac ttttgttttc ctcggggcgt cgacaggctc
       61 ggtcatagca cactattata cgcgatttgg cgcgggggcg gggagaaaaa tgcgggccgg
      121 ggaagcgtcc gcgacccgac cgccgcgggg cgcgcgcggc gccgcggaaa cgggaaatcc
      181 tccttgcccg ccgcgcggtt tggggcgtta ttacgcggcg atggacaagg cggataaggc
      241 gccgaccgca ccggtcctag cgctcgtggg tatgccgggg tcgggaaagt cgaccgccgc
      301 ggagatgctg gccgcgcggg ggtgggcgac gatccggttc gggcaggtga cgatcgacga
      361 aatcgcggcg cgcgggctga cggtcgatga gcgcaacgag cgggcgatcc gcgaggaact
      421 gcggcgcgtg cacggtccgg acgcctacgc ccggctggcc atgccgaaga tcctcgcagc
      481 ccgggagacc gggagagtgc tgatcgacgg gctgtactcg tggtcggagt acaagtacct
      541 ccacgaggca ctggccagcc ggatgctcgt gctggcgatc acctcggccc gcaggatccg
      601 gtacgcccgg ctggccggcc gcagcgagcg gccgctcacg gccgaggagg cgcgccggcg
      661 ggatttcgcc gaggtcgaga acctcgaaaa aggcgggccg atcgccatgg ccgactacac
      721 tatcgtcaac gacgccgcgc tcgacgacct ccggcgggag gttgaccggg ccatcgcgtt
      781 tctcttcccc tcctgacatg atggacgagc gggcgatcaa aaagctgctg gcccaggagc
      841 ggcagcgctg gttcgggtgg gggcacgagg actggcgcga aatgatcgag gcgaccccgg
      901 atgacgaaga tggggagctc ttcttctacc tcgcgccctt cggcggcgag gcctttccgg
      961 tcgaggtcgt ctgtttcccc gacccggacg gggcgatgga gatctcgctc attgtgacca
     1021 tcgaggagaa cgggcgggag gcggaggcgg cttggatggt gtacgccgac ggggagaaag
     1081 aaatgctggt ggagacgacc gaccggcggg agggggacga ggagggtgat gagtaagggg
     1141 ccgcagacgg ggcggctggc gccgcccgat atcaaccgcc tcctccagga ggaacggctg
     1201 cggtgggcgc gccgcccgta cgagtttctc cgcacggttc cgtcgcaccc cgaggggccg
     1261 ctcgcctact accggttccg atcggggcgg acgtttttcg tgcgcctggc agtggtgtcg
     1321 gccaccgagg cccgggtgga gctgcgggtg acggccgagg aacaggcggg gtgtctgggg
     1381 ttcgggcggc ggccggcggg gaaggaactc acgttcacgg ttctgcggcc ggtctgaggg
     1441 ccgcggcgca gggggggtca ttcttccggc cgaaggagtt gctcggcggc gagacaggcg
     1501 gctggcgagc ccagcatgta caggacatcc tcggcttcca cgaccgcgtc ggcgccggga
     1561 ttggccgtca cctgctcgcc gcggcggatg gcgaggatgt tgacgccgca gcgggcgcgc
     1621 aggttcaact gggccaggtt gcggccggcg aagggggaat cgggcacgac gcgcacagac
     1681 gtgatttcga cgtcgctgat gccggccgga aggtcggcca tgcggggggc ggggtgggac
     1741 acggtgcgga gcatctcata gctgtccgac cggatt
//
LOCUS       DAOOKS010000471         1774 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_85427_length_1774_cov_3.007563, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000471 DAOOKS010000000
VERSION     DAOOKS010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85427_length_1774_cov_3.007563"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /gene="fmt"
                     /locus_tag="PK112_07585"
     CDS             complement(<1..910)
                     /gene="fmt"
                     /locus_tag="PK112_07585"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HPC11944.1"
                     /translation="MGTPEYACPTLACLVHSEHEVAAVVTGPDVRPPRGGGLHPTPVK
                     REALRHGLTVLTPATLRNNPALREALAGLKADVFVVVAFKILPRSLYALPRLGSVNIH
                     ASLLPRYRGAAPIHWALINGETETGLSSFVLNDTVDTGDLVLQERVPIDPADTFDSLY
                     ARMAALSGPFALRSLERLADPAFAPIPQDGTAATPAPKVTPEDALIDWGFPAPLVHNF
                     IRGLSSVPGAYSTFRSRRVKILGSRPVVDAPAAPGAPPGAIVPDRRRLLVQCASSALE
                     LTRLVPEGKKGMDGVSFLNGLQPQPGE"
     gene            complement(1004..1513)
                     /gene="def"
                     /locus_tag="PK112_07590"
     CDS             complement(1004..1513)
                     /gene="def"
                     /locus_tag="PK112_07590"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HPC11945.1"
                     /translation="MAVRPIVIYGDPVLREKAQPVETIDREIKDLVSDLTDTLKEAQG
                     LGLAAPQIGVSRRVFIIDLSVIDINADLKVFINPEIIEAGGEPVEYEEGCLSFPGLYQ
                     KITRPAWVKVRAIDINGNAFEIATDGMLARAIQHEFDHLEGILFIDRMSALTRTMIQG
                     RLRKLQKVS"
     gene            complement(1528..>1774)
                     /gene="yajC"
                     /locus_tag="PK112_07595"
     CDS             complement(1528..>1774)
                     /gene="yajC"
                     /locus_tag="PK112_07595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HPC11946.1"
                     /translation="IWFGLIFVVMYLLLIRPQRKKQKEHEHLLKDLKKGDKVITSAGM
                     FGVIFAIDDERGRVVLKFGENVKMEFLKSSIAARVDS"
BASE COUNT          335 a          539 c          580 g          320 t
ORIGIN      
        1 gctcgcccgg ctgcggctgc aggccgttga gaaaggacac cccgtccatc cccttcttcc
       61 cctccgggac caggcgagtc agctcgaggg ccgagcttgc gcactggacc aggaggcggc
      121 gccggtcggg cacgatggcg ccgggcggtg cgcccggggc cgccggggcg tcaacgaccg
      181 ggcggctgcc gagaattttc acgcggcggc tgcggaaggt ggagtaggcg cccgggacgg
      241 agctgaggcc gcggatgaag ttgtgcacca gcggcgcggg aaagccccag tcgatgaggg
      301 cgtcctcggg ggtgactttc ggggccgggg tcgcggccgt gccgtcctgc ggaatggggg
      361 caaacgccgg gtcggcgagc cgctccaggc tgcgcagggc aaacggcccg gacaacgcgg
      421 ccatccgggc atagagcgaa tcgaaggtgt cggccggatc gatcggaacg cgctcctgga
      481 gcacgagatc gccggtgtcc accgtatcgt tgagaacaaa actagagaga ccggtttcgg
      541 tctcgccgtt gatgagcgcc caatggatcg gtgcggcgcc gcggtaccga ggcaaaagcg
      601 acgcgtggat gttgaccgag cccaggcggg ggagcgcgta gaggctgcgg gggaggattt
      661 tgaaggcgac gaccacgaaa acgtcggcct tcagccccgc cagcgcctca cgcagcgccg
      721 ggttgttcct gagcgtggcg ggggtcagga cggtcagacc gtgccggagc gcctcgcgct
      781 tgacgggcgt ggggtgcaga cccccgccgc gcggcggacg cacatcggga ccggtcacca
      841 ccgcagccac ctcatgctcg ctgtgcacca ggcaggccag agtggggcat gcgtactccg
      901 gcgtgcccat aaacaccaat ctcatgcgga ctgtggattg cggcggagcg acgattctca
      961 ttccgggaac cgtcggacaa gatcaacgcg cgggcgaata tcgtcaggaa accttctgca
     1021 gctttctcag gcgcccttga atcatggtcc gagtcagagc ggacatgcgg tcgatgaaca
     1081 ggattccctc caggtggtcg aattcgtgct ggattgccct cgccaacatg ccgtccgttg
     1141 cgatctcaaa cgcgtttcca ttgatgtcga tagcccgaac cttcacccag gccgggcggg
     1201 tgatcttctg atatagtccc ggaaaggaca ggcaaccctc ctcatattcc accggttcgc
     1261 cgcctgcctc gataatttcc ggatttatga agaccttgag gtcggcgttg atgtcaataa
     1321 ccgacaggtc gatgatgaaa acgcgcctgg acacgccgat ctggggtgcc gccagaccga
     1381 gtccctgcgc ctctttgagc gtgtcggtaa ggtccgacac caagtctttg atttcccggt
     1441 cgatcgtttc gaccggctgc gccttctccc gcagtaccgg gtcgccgtaa atgacgatcg
     1501 gtcgcaccgc cacaataact ccttacacta gctgtcgacg cgcgcggcaa tggaggattt
     1561 cagaaactcc atcttgacgt tttcgccgaa tttcagcacg actcggccgc gttcgtcatc
     1621 gatggcgaaa atcactccaa acatgccggc ggacgtgatc actttgtcgc cctttttcag
     1681 gtctttgagc agatgttcat gttccttttg ctttttgcgc tgcggccgga tgagcagcaa
     1741 atacataacg acgaagatga gaccaaacca gatg
//
LOCUS       DAOOKS010000472         1772 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_85623_length_1772_cov_2.493885, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000472 DAOOKS010000000
VERSION     DAOOKS010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85623_length_1772_cov_2.493885"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..788)
                     /locus_tag="PK112_07600"
     CDS             complement(<1..788)
                     /locus_tag="PK112_07600"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HPC11947.1"
                     /translation="MRTVCIAGVGMSQWGEVWRTSLRQLFADAAGEAIKNAGVDHLDS
                     LYVGCMSGGLFVQQEHLGSLMADYMGMPGLPAMRVESACASGGMALRAAYFEVAAGAA
                     EIVMAGGAEKMTDCTGDQATAALATAADQEYEVFNGATFPGLYAMMAHAHMARYGTTR
                     AMLTAVAVKNHRNGSMNPFAQYPFPVKPETVESSVMVADPLRILDCSPITDGAAAAIL
                     CSEEVAKKLGRPYVRIIGSGAATDTIQLAQRADLTTIKAAALATA"
     gene            complement(800..1711)
                     /locus_tag="PK112_07605"
                     /pseudo
     CDS             complement(800..1711)
                     /locus_tag="PK112_07605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00748.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
BASE COUNT          278 a          613 c          552 g          329 t
ORIGIN      
        1 gccgtcgcca gggcggccgc tttgatcgtc gtgaggtcgg cccgctgggc caactggatc
       61 gtgtcggtcg ccgcgccgga cccgatgatc cgcacgtacg ggcgtccgag ctttttggcg
      121 acttcctcgc tgcacagaat cgccgctgcc gccccgtcgg tgatcggcga gcagtcgaga
      181 atgcgcagcg ggtcggccac catgaccgag ctctcgaccg tctccggttt caccggaaac
      241 gggtactggg cgaatggatt catcgacccg ttgcggtggt tctttaccgc cacggccgtc
      301 agcatcgccc gcgtggtccc gtacctggcc atgtgcgcgt gcgccatcat ggcgtagagc
      361 ccggggaagg tggccccgtt gaacacttcg tactcctggt cggccgcggt ggccagcgcc
      421 gcggtcgcct ggtcgccggt gcagtcggtc attttctcgg ctccgccggc catcacaatc
      481 tcggccgccc ccgcggccac ctcgaaatag gccgcccgga gcgccatgcc ccccgaggcg
      541 caggcgctct cgacccgcat cgccggcagc cccggcattc ccatgtagtc cgccatgagc
      601 gacccgagat gctcctgctg gacaaacaac ccgcccgaca tgcaccccac gtagagggag
      661 tcgagatggt cgacgcccgc gttctttatc gcttcaccgg ccgcgtcggc aaagagctgg
      721 cgcagcgagg tgcgccacac ttcgccccac tggctcatgc cgaccccggc gatacacacc
      781 gttctcattc tttctcctcc tacgcgttct tgcggatttt gcgccggaac ttggcatagg
      841 tgccgtagtc gacataggtg aggttttcat cgagcagctt gtgcgtcctg attgtcaggt
      901 cccgcacctt gtcgatccgg tccgtgacct tccagatgaa cgcatccgag cccgcgcccg
      961 aaccgtatga acacatcatg atcaggtccc ccggtttggc cgtgtcgagc gtcgccgtca
     1021 ggccgatcgg cgaggctccc gagtaggtgt tccccaagcg gggggacagc cagcccggat
     1081 cgatctgcgc ctgggtgaac cccagttcca gcgccacccg ctgcgggaac ttcccgttcg
     1141 gctggtggaa caccgcccac tggaaatcct gcggcttcat ccccgatttc ttcaggatgg
     1201 cggtggtagc gcctttcgtg tgcgcgaaat aggcctcttc gccggtaaac cggccgccgt
     1261 gctgcgggta gaactcgtgc tcgcggcgcc agaaatcggg catgtcggtc atgtaggaat
     1321 gggtgtagag acattcggcg atgaggttgt ccttgcccat gatgaacgcc ccggccccgg
     1381 ccgcggccga gaattcgagc gcgtccgagg gagcgccctg tgaggtgtcg gccgcgatcg
     1441 ccagcgcgta cttcatgaac cccgactcca cgtggctgag cgcgacaaac atcgcctccg
     1501 accccgcttt gcaggcgaac tcaaaatcgg cgcagtgcag atccggcgtc gccccgatcg
     1561 cctcccccac cgtcgttccc gagggcttca ccgcgtaggg gtgcgattcc gagccgatgt
     1621 agatcgcccc gatatctttc gggtccaccg tctgcgcccg aatgagggcg cgccgggccg
     1681 cctccaccga cagcgtaatg gtgtccatgt cgggcggcgg aacaggcccg aagctgttgc
     1741 gccacgttta cacggagcac ttcgcgaatg cg
//
LOCUS       DAOOKS010000473         1770 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_85765_length_1770_cov_2.812245, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000473 DAOOKS010000000
VERSION     DAOOKS010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85765_length_1770_cov_2.812245"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            31..>1770
                     /locus_tag="PK112_07610"
     CDS             31..>1770
                     /locus_tag="PK112_07610"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HPC11948.1"
                     /translation="MPASDSTGLPVIRTYLSALVVFFLLRLGFLAWHAALFRGAAGTE
                     LARAFLGGAVIDSSVTACTLLAAWLIALAALAAGRTAARRVFLGLCAILFGLYFFINL
                     ADIAYFDMFDSRLNVLVFENLDQMAPILQTIASGWSVVIVTIVWLAVLTAFILALRAR
                     SRVGGYSRRGARRVISGITVGLLLAALTFLWVGEPFWRLGLFSSGNQALTQLSLNGVY
                     TLLKAYHQKRIWERDFGDIDYAFGPPEEALQRVRREIIGDDEAASEGRFPFARELRRP
                     APLAVAKPNIVIILMEGFTASGVGALCPGGIGDSPGFDRLAHEGILFTNFYGAGTRTH
                     HGLVSTVASFPSLLELFLTRRRGTESFSTLGTLLRAYGYRTSFFYGYDAGFDHMGFFM
                     RQGGFDLIVDQRDFAAPRFVGKWGVSDEDLFAKVSAYLSGQAEGEPVLSVVMTSSNHT
                     PHDIPPEFAAARPQYAGDRARAAFAYSDYALARFLDGERDKPYFRRTIFVIVADHGEI
                     RDRADRYFKRFQIPCLLYAPGLIPGGRTVSTVGSQADIATTLMHLIGYPRPFHFLGRD
                     LLALPEDRGFAVMR"
BASE COUNT          239 a          598 c          574 g          359 t
ORIGIN      
        1 gcgtcgccga ccattcacag gcagggtacc atgccggcgt ccgacagcac cggtcttccg
       61 gtcatccgca cttacctgag cgcgcttgtg gtgttcttcc tgctgcgcct cgggtttctc
      121 gcctggcacg cggcgctttt ccgcggcgcg gcggggacgg aactggcgcg ggcctttctc
      181 ggcggggcgg tgatcgattc ctcggtgacc gcgtgcaccc tcctggcggc gtggctgatc
      241 gcgctggcgg cgctcgccgc gggccgcacc gccgcgcggc gcgtcttcct cggcctgtgc
      301 gcgattctgt tcggcctcta cttcttcatc aatctggccg atatcgccta ctttgacatg
      361 ttcgactccc gcctgaacgt cctcgttttc gaaaacctgg accagatggc gccgattctg
      421 cagacgatcg cctccgggtg gtcggtcgtg attgtcacga tcgtgtggct ggcggtcctg
      481 accgctttca tcctggcgct gcgcgcccgc tctcgggtcg gcgggtattc ccgccgaggg
      541 gcgcgccggg tcatctcggg gatcaccgtc ggactgcttc tcgccgccct caccttcctg
      601 tgggtgggcg aacccttctg gcggctcggc ttattctcct ccggcaacca ggcgctcacc
      661 cagctctcgc tgaacggcgt gtacacgctc ctcaaagcct accatcagaa gcggatctgg
      721 gagcgcgact tcggggacat cgactatgcc ttcgggcctc cggaggaggc gctgcagagg
      781 gtgcgtcggg agattatcgg cgacgatgag gcggcgagcg agggaagatt tccgttcgcc
      841 cgggaacttc gccggcccgc gcccctcgcc gtggccaaac ccaacatcgt gatcattctc
      901 atggaagggt tcaccgccag cggggtggga gcgctctgtc ccggcgggat cggcgactcg
      961 cccgggttcg accgcctggc gcacgaggga attctcttta cgaacttcta tggcgccggc
     1021 accaggacgc accacggtct ggtcagcacg gtggcctcgt tcccctcgct cttggagttg
     1081 ttcctcacgc ggcggcgggg gacggagtcg ttctcgacgc tggggacgct gctgcgcgcc
     1141 tacggatacc ggacgagttt cttctacggc tacgatgccg ggttcgacca catgggtttc
     1201 ttcatgcggc agggagggtt cgatctgatc gtcgatcaaa gggacttcgc cgcgccccgc
     1261 ttcgtcggca agtggggcgt ctccgatgag gacctgttcg ccaaagttag tgcgtacctg
     1321 tccggccagg ccgaggggga gccggtgctg agcgtggtta tgacgtcgtc caaccacacg
     1381 ccgcacgata ttcccccgga gttcgcggcc gcgcggccgc agtatgccgg agaccgggcc
     1441 cgggccgcct tcgcctactc cgactacgcc ctcgcgcggt ttttggacgg agaacgtgac
     1501 aagccctatt ttcgccgcac gatcttcgtg atcgtcgccg atcacgggga gattcgggac
     1561 cgagccgacc gctatttcaa gcgctttcag attccctgcc tgctctacgc gccggggttg
     1621 attccgggag gccggaccgt gtcgaccgtg ggcagccagg cggacatcgc cacgacgctc
     1681 atgcacctga tcggctaccc ccggcccttc cacttcctcg gccgcgatct cctggcgctt
     1741 cccgaagacc ggggattcgc cgtcatgcgg
//
LOCUS       DAOOKS010000474         1769 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_85826_length_1769_cov_3.600933, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000474 DAOOKS010000000
VERSION     DAOOKS010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85826_length_1769_cov_3.600933"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="PK112_07615"
     CDS             <1..334
                     /locus_tag="PK112_07615"
                     /inference="COORDINATES: protein motif:HMM:NF012550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HPC11949.1"
                     /translation="ADILKGKQVHQDCRVLVVPASRSVYLEALKKGLIRVFAEAGAAV
                     TDPSAGLNAARLPEQVAPGERCLASTNTSLFPRAAELGAELYLCSPATAAASALNARI
                     TDPTPYVR"
     gene            complement(357..1478)
                     /locus_tag="PK112_07620"
     CDS             complement(357..1478)
                     /locus_tag="PK112_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020026385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HPC11950.1"
                     /translation="MNPLPLEPPVRTLLGPGPSDPYPEVLAAIARPTVGHLDPWFIDL
                     MDRTRELLRYAFRTDNELTIALSGPGSAGMETCLANLIEPGDTAVVCVNGYFGSRMRD
                     IVDRIGGRPVAVNDEWGRPVDPAKVEDALKKNPGAGILAFVHAETSTGAQSDAKTLAE
                     LARRHGYLALVDAVTSLAGTPLYVDDWQIDAVYSGSQKCLSCVPGLAPLSLSERALDR
                     VRRRRTPVAGWFSDLTLLAQYWSGGAKRTYHHTAPVNSIYALYAALERLRSEGLENSW
                     HRHRDNHRLLKTGLESLGLEFLVDESARLPQLNAVRVPTGVDEAAVRRRLLEEFNIEI
                     GAGMGELAGKIWRIGLMGCSSDPLKIERLLAALRKILVA"
     gene            complement(1653..>1769)
                     /locus_tag="PK112_07625"
     CDS             complement(1653..>1769)
                     /locus_tag="PK112_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_083760882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HPC11951.1"
                     /translation="RKTKGAEVTPAEWEPGRAVLKVGPSLVGNVWKEWKRSG"
BASE COUNT          276 a          582 c          625 g          286 t
ORIGIN      
        1 cgcggatatc ctcaagggca aacaggtgca ccaggactgc cgggtgctgg tggtcccggc
       61 ctcccgctcg gtgtacctgg aggcgctcaa gaaaggattg atccgcgtgt ttgccgaggc
      121 gggggcggcg gtcacggatc cttcggcggg gctgaacgcc gcgcggctgc cggaacaggt
      181 tgcgccgggg gaacgatgcc tggccagcac caacacctcg ttgttccccc gggcggccga
      241 gctcggcgcg gagctgtact tgtgctcgcc ggcgaccgcg gccgcgtcgg ccttgaacgc
      301 ccggatcacc gacccaacgc cgtacgtgcg gtaggggggg caggaagaac ccggcttcag
      361 gcgacgagga tctttctcag cgccgccagc aggcgctcga tcttgagggg gtccgaggaa
      421 cagcccatca gtccgatgcg ccagattttt ccggccaatt cgcccatgcc ggcgccgatc
      481 tcgatgttga actcctcaag gagccgccgc cgcaccgcgg cttcatcgac accggtcggc
      541 acgcgcaccg cgttgagctg cggcagccgg gcgctctcgt cgaccagaaa ctccaacccc
      601 agcgactcca ggccggtttt caggagccgg tggttgtcgc ggtggcgatg ccaactgttc
      661 tcgagacctt cgctgcggag ccgctccagc gcggcgtaga gggcgtagat cgagttgacc
      721 ggggcggtgt ggtggtaggt gcgtttcgcc ccgcccgacc agtactgcgc caggagagtg
      781 aggtcggaga accagccggc caccggggtc cggcgccgcc gcacccggtc gagggcgcgt
      841 tcgctgagac tgagcggcgc gaggcccggc acgcaggaga gacacttctg cgagccggaa
      901 tagacggcgt cgatctgcca gtcatcgaca tagagcggcg tcccggcaag cgacgtgacg
      961 gcatcgacga gggccaggta cccgtgccgg cgggccagct cggcgagcgt tttcgcatcc
     1021 gactgcgcgc cggtcgacgt ctcggcatgg acgaaggcga gaattccggc tccggggttc
     1081 ttcttcagag cgtcttcgac tttcgccgga tcgaccggcc ggccccattc gtcgttcacc
     1141 gcgaccgggc gcccgccgat ccggtcgaca atgtcgcgca tgcggctgcc gaagtagccg
     1201 ttgacgcaca cgaccgcggt gtcgcccggc tcgatgagat tcgccaggca ggtctccata
     1261 ccggccgagc cgggaccgga cagcgcaatg gtcagttcgt tgtcggtccg aaaagcgtag
     1321 cggagcagtt ccctcgtccg gtccatcagg tcgataaacc aggggtcgag gtggccgacc
     1381 gtcgggcggg cgatggccgc gagcacttcc ggatacgggt ccgaggggcc ggggcccagg
     1441 agcgtgcgga ccggcggttc gaggggcagg ggattcatga ggtcgggtct ccgggactaa
     1501 gtcttcgttc gtcctcgacg ctcgcacggg ggcggcgatc gccgatgcgc gatggtgaaa
     1561 tgccgccggg tccgccgcac ctccggaaaa gagccgggtt tatatccaaa cccggctcgg
     1621 agtacgcggc gggacggctc tggattgcag cctcagccgc tccgcttcca ttctttccag
     1681 acgttgccga cgagactcgg gccgactttg agcacggcgc gtcccggctc ccactcggcc
     1741 ggcgtcacct ccgcgccttt ggtcttgcg
//
LOCUS       DAOOKS010000475         1769 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_85861_length_1769_cov_2.128355, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000475 DAOOKS010000000
VERSION     DAOOKS010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85861_length_1769_cov_2.128355"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..>1769
                     /locus_tag="PK112_07630"
     CDS             158..>1769
                     /locus_tag="PK112_07630"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HPC11952.1"
                     /translation="MKSPDQKKFRRDRLRTMLVGLVALALLLLAVAAYKTYRVYQSDL
                     PSFEQLHNIEPSLKTKIYDRNGILIQEFFSENRALTPFDQMPPDLVNMLLASEDRKFY
                     DHWGIDVRRVLVVAFSNLLSWDIKAGASTITQQLARMLFLNREQTFDRKFKEALTAVK
                     LERTYSKQEILEMYLNQYYFGSGAYGVAAAAHNFFGKNVAELTISDDAVLIGMLKAPN
                     SNSPFRYPDRALKARNRVLYSYYTYGGLNRAQYDSLKDEPLVVTPPSENVGDAPYFTE
                     EIRQYIFDRYGESMLYSGGLKVFTSLDVGLQRAAEAAVARKVDSLRARIERMYTLGNP
                     DYTELLPDTVDAQGRPIRAYKHVQGAFVAIDNATGDVLALVGGRSFEATKYNRATQGR
                     LQPGSAFKPFVYTAAVDNGFRTSDLIDDLPIVLDIPGASQWRPHNFDDKFMGPITLRD
                     GIRLSRNLVAIRLLLRIKPEQAIFYARRMGITTPLQANPSLALGTSEVIPIELVSAFS
                     VFPNKGIHIPYRMIHKIVDRYGSVIEDNT"
BASE COUNT          344 a          625 c          480 g          320 t
ORIGIN      
        1 cgtatcgaca tataatcgga aaagcctcag cggcggccgc ctggtcggaa ggctgctgcc
       61 gcatggtgaa caataaacgg ccgaggctcc ggcggaacaa gcgggatttt gttttagctt
      121 gcgtgtccgg gagaccggcc tgttattgac agacaggatg aaatcgcccg atcaaaagaa
      181 gttccgccgc gatcgcctcc gcaccatgct ggtcggcctg gttgccctgg ccctcctgct
      241 cttggcggtc gccgcctaca agacctaccg cgtctaccaa tccgatctcc cctcgttcga
      301 gcagctccac aacatcgaac cgagcctgaa aaccaagatc tatgaccgca acggaatcct
      361 cattcaggag ttcttcagtg aaaaccgcgc cctgaccccc tttgaccaga tgcctcccga
      421 cctggtcaac atgctcctgg cctcggagga ccggaagttc tatgaccact gggggatcga
      481 tgtgcgccgc gtcctcgtgg tcgccttctc caaccttctg agctgggaca tcaaggccgg
      541 cgcttcgacc atcacccagc agctcgcccg gatgctcttc ctcaaccgcg agcagacgtt
      601 cgaccgcaag ttcaaggagg ccctcaccgc cgtcaaactc gagcggacct attccaagca
      661 ggaaatcctc gagatgtacc tgaaccagta ctatttcggc agcggcgcct acggtgtcgc
      721 cgccgccgcc cacaatttct tcggaaagaa cgtggcggag ctgacgatca gcgacgacgc
      781 ggtgctgatc ggcatgctca aggctcccaa cagcaactcc cccttccggt atcccgaccg
      841 ggccctcaaa gcccgcaacc gcgtcctcta ttcctactac acgtacggcg gattgaaccg
      901 ggcccagtac gattcgctca aggacgagcc gctcgttgtc accccgccct cggaaaacgt
      961 cggcgacgcc ccctatttca ccgaggaaat ccgccagtac atctttgacc gctacggtga
     1021 gtcgatgctc tattccgggg ggctgaaggt gttcacgtcg ctcgacgtcg gtctgcagcg
     1081 cgcggccgag gcggccgtgg cccgaaaggt ggattcgctc cgcgcccgga tcgaacggat
     1141 gtatacgctg ggcaatcccg actacaccga gctgcttccc gacacggtcg acgcccaggg
     1201 gcgccccatc cgcgcctaca agcacgtgca gggggccttt gtcgccatcg acaacgccac
     1261 cggcgacgtt ctcgcgctgg tgggcgggcg gtcgttcgag gcgaccaaat acaaccgggc
     1321 cacacagggc cggcttcagc ccggctcggc cttcaaaccg ttcgtctaca cggcggccgt
     1381 cgacaacggt ttccgcacct ccgacctgat cgacgatctc cccatcgtcc tggacatccc
     1441 cggcgccagc cagtggcggc cgcacaattt cgatgacaag ttcatggggc cgatcacgct
     1501 ccgcgacggc atccggctct cccgcaacct cgtcgctatc cgcctccttc tccggatcaa
     1561 accggagcag gccatcttct acgcccgccg gatgggcatc accaccccgc tccaggcgaa
     1621 cccctccctc gccctcggca cgagcgaggt gattcccatc gagctggtgt ccgccttttc
     1681 ggtctttccg aacaagggga tccacatccc ctaccggatg atccacaaga ttgtcgaccg
     1741 ctacggcagc gtcatcgagg acaacacgg
//
LOCUS       DAOOKS010000476         1760 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_86473_length_1760_cov_4.285044, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000476 DAOOKS010000000
VERSION     DAOOKS010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86473_length_1760_cov_4.285044"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /gene="rimI"
                     /locus_tag="PK112_07635"
     CDS             <1..461
                     /gene="rimI"
                     /locus_tag="PK112_07635"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HPC11953.1"
                     /translation="NDLVLRDMAARDLDTVVAMEKEIFPDPWPRSAFEEQIGGDGWGA
                     LVVEIDGAVVGYACYFTVDVEAHLTNIAVRPEHRRKSVANMLLGAILGRATAAGCQYL
                     LLEVRSSNNVARTFYERKGFRILYRRPNYYRRPIEDALVMVFYLDGRQGG"
     gene            467..1309
                     /gene="accD"
                     /locus_tag="PK112_07640"
     CDS             467..1309
                     /gene="accD"
                     /locus_tag="PK112_07640"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HPC11954.1"
                     /translation="MAWFRKDHSGPVPQEKKNIPEGLWIKCDSCGEIIYSRKLEKLLW
                     VCPSCDFHFRITSQKYIDLLLDGGRLEEFDSHLTSRDPLKFKDSKKYPDRIREAQAKA
                     GRLEGVIAGIGEIGGIEVSFAIMNFAFIGGSMGSVVGEMIARAIERALNRNIPLVIVS
                     SSGGARMQEGILSLMQMAKTSGLLERLDKKRIPYISILTNPTTAGVMASYASLGDVVI
                     AEPKALLGFAGPRVIQQTIKQELPEGFQSSEFFLEKGFLDKIVHRKDLRDTVIALLKF
                     MWRK"
     gene            1297..>1760
                     /locus_tag="PK112_07645"
     CDS             1297..>1760
                     /locus_tag="PK112_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HPC11955.1"
                     /translation="MEKVMPAGAYRTAERFLLSREFFGMKLGLENITEFLAAIGSPQL
                     SYPTIHVAGTNGKGSTVSMLAAVLRAQGYRTGLYTSPHLVSLRERVRVDGHPIPERSV
                     TAFVARYRRELVRRKLSFFEVLTAMAFEHFARAGVDLAVVETGLGGRLDATNV"
BASE COUNT          329 a          599 c          509 g          323 t
ORIGIN      
        1 aaaatgacct cgtcctccgg gacatggccg cacgcgacct cgataccgtc gtcgccatgg
       61 aaaaggaaat cttccccgat ccctggccgc gctccgcgtt cgaggagcag atcgggggcg
      121 acggctgggg ggcgcttgtg gtcgagatcg acggcgccgt ggtcggctac gcctgctact
      181 tcactgtcga tgtcgaggcc cacctcacca acatcgccgt ccgccccgaa catcgcagaa
      241 aatcggttgc aaatatgttg ctgggcgcta tcttgggtcg ggcaaccgcg gccggttgcc
      301 agtatctgct cctggaggtc cggtccagca acaacgttgc ccggaccttt tacgaaagaa
      361 aggggttccg aatattgtat cgccggccca attactaccg gcggcccatt gaggatgccc
      421 tggtcatggt cttctacctc gacggccggc aaggcggata gacgctatgg cgtggtttcg
      481 gaaagatcac agcggcccgg tgccgcagga aaagaaaaat atccccgagg gtctctggat
      541 caagtgcgac tcctgcgggg aaatcatcta cagccgaaaa ctcgaaaaac tcctctgggt
      601 ctgcccctcc tgcgacttcc acttccgcat cacctcccag aaatatatcg acctgctgct
      661 cgatggcggg cggctggagg aattcgacag ccacctgacg agcagggacc cgcttaagtt
      721 caaggattcc aagaagtacc cggatcggat cagagaggcc caggccaagg ccggccgcct
      781 cgagggtgtg atcgccggca tcggcgaaat cggcggcatc gaggtctcct tcgccatcat
      841 gaacttcgcc ttcatcggcg ggtcgatggg ctcggtggtg ggggagatga tcgcccgggc
      901 catcgaacgc gccctcaacc gcaacatccc cctcgtcatc gtctcctcct ccggcggcgc
      961 ccgcatgcag gaggggattc tctcgctgat gcagatggcc aagacctccg gcctgctcga
     1021 acgactcgac aagaaacgca tcccctacat ctctatcctc accaacccga ccaccgccgg
     1081 cgtcatggcc tcgtatgcgt cgctcggcga tgtcgtcatc gccgaaccga aagccctcct
     1141 cggattcgcc ggcccgcgcg tcatccagca gaccatcaaa caggagctgc cggagggctt
     1201 tcagtcgtcc gagttctttc tcgaaaaggg gttcctcgac aagatcgtgc accgcaaaga
     1261 cctccgcgac accgtcatcg cgctcctgaa gttcatgtgg agaaagtgat gcccgccggc
     1321 gcctatcgca ctgcggaacg gttcctcctc tcgcgcgagt tcttcggcat gaaactcggg
     1381 ctggagaaca tcaccgagtt cctcgccgcc atcggctccc cccagctgag ctacccgacc
     1441 atccacgtgg ccggcaccaa cggcaagggc tccaccgtca gcatgctcgc cgctgtcctc
     1501 cgggcccagg ggtaccggac cggcctgtac acctctcccc acctcgtctc gctgcgggag
     1561 cgggtgcgcg tcgacggcca tccgatcccg gaacgctccg tcaccgcctt tgtcgcccgc
     1621 taccgccggg aactggtccg gcgcaagttg tcgtttttcg aggtgctcac cgccatggcc
     1681 ttcgagcatt tcgcccgcgc cggggtcgac ctcgccgtgg tcgaaaccgg tctcggggga
     1741 cggctcgatg cgaccaacgt
//
LOCUS       DAOOKS010000477         1758 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_86653_length_1758_cov_3.192014, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000477 DAOOKS010000000
VERSION     DAOOKS010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86653_length_1758_cov_3.192014"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="PK112_07650"
     CDS             complement(<1..580)
                     /locus_tag="PK112_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11956.1"
                     /translation="MSTRNRQAALAVLLLCLFGLSAGGADAVNYSVANRGRVADNASE
                     IIMPADGSPKAPFSGALRLYVVEPESRWDDADGLPYHFATIDIALDTSIALNDGDRYV
                     RSRFWEGTTWAMTEQNAMVIAVLFEGTPHTAYSDPPSGGVFNAYYAEAAAYARPGFCD
                     SNNTSGNSTHTVFIEEGTAVWCPYCPDPNYWLY"
     gene            complement(645..>1758)
                     /locus_tag="PK112_07655"
     CDS             complement(645..>1758)
                     /locus_tag="PK112_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11957.1"
                     /translation="NGYTNNLLSEASAQDDAYSTARLTARWYPLPQLQLSLGTDYTYY
                     NNVIGLGNNTPRAGLIWVPTDPAGRLTFYLEGNTSARRYRAAYSSYSTNEGYLKGSVG
                     FRLNPRFLLRSGIKFNGTNYITPDSTTDADYEQYELFAGVNIALPWSNSFDLEAGLGQ
                     TNFALIDAAKNPDFQNPYAAPGVQWPATKFLEDGDFRALYVSPRVSRPLGDKTGISLT
                     YVYRQFLDVDEAVVLGITTDFLSPWTSFFDGSSLQLRIKSYVISDFILTGGLGYWDKT
                     FLRSTEVVVDTIPTPRPDDPDRVRIETRWVDPKAATAREDDMTRLYFGLQRPFTFRGG
                     LYLEPSLGLEYIDNRSTRDNFDYSTTAVTFNILVRP"
BASE COUNT          326 a          479 c          625 g          328 t
ORIGIN      
        1 agtagagcca gtagttcgga tccgggcaat acggacacca gaccgccgtg ccttcctcga
       61 tgaacacggt gtgggtggag ttgccggagg tgttgttgct gtcgcagaaa ccggggcggg
      121 cgtaggccgc cgcttccgcg tagtacgcgt taaagactcc gccggacggc ggatcgctgt
      181 aggcggtgtg cggggtgccc tcaaacagca ccgcgatcac catcgcattc tgctccgtca
      241 tcgcccaggt cgtcccctcc cagaagcggc tgcggacata gcggtcgccg tcgttgaggg
      301 cgatggacgt gtcgagggcg atgtcgatgg tggcgaagtg gtaggggagt ccgtcggcgt
      361 cgtcccagcg cgactccggc tccacgacat agaggcgcag ggcgccgctg aagggcgcct
      421 tgggcgatcc gtcggcgggc atgataatct cggacgcgtt gtcggccacg cgccccctgt
      481 tcgcgacgct gtagttgaca gcgtctgcgc cgccggccga aaggccgaac aggcacaaca
      541 gcagcacggc aagggctgcc tgacggttgc gggtactcat tccgggatcc tttctcgtca
      601 atcggacaat cggtggttct gttgccaagg taccgttaac ggcctcacgg ccggacaagg
      661 atattgaagg tcacggccgt ggtcgaatag tcgaaattgt cgcgcgtgga gcgattgtca
      721 atgtactcca ggcccaggct gggttcgagg tacaatccgc cgcggaacgt gaacgggcgc
      781 tggagaccaa aatagagccg ggtcatgtcg tcttcgcggg cggtcgcggc cttgggatcc
      841 acccagcgcg tttcaatccg gacgcggtcg gggtcgtcgg ggcggggcgt cggaatggtg
      901 tccactacca cctcagtcga gcgcaggaag gtcttatccc agtagcccag gccgccggtc
      961 agaatgaaat cggaaatcac atagctctta atgcgcagtt gcagcgagga gccgtcgaag
     1021 aaacttgtcc acggcgacag gaagtcggtc gtgatgccga gaaccacggc ttcgtcgaca
     1081 tcgaggaact gccggtaaac gtaggtgagg ctgatgccgg ttttgtctcc gagcggccgc
     1141 gagacgcgcg gagagacgta gagagcacga aagtctccat cctcaaggaa cttggtggcc
     1201 ggccactgca cgccgggcgc ggcgtagggg ttctgaaaat ccgggttctt ggccgcgtcg
     1261 atcagagcaa agttggtctg gcccaggccg gcttcaagat cgaaggagtt actccacgga
     1321 agagcgatgt tgacgcccgc aaagagctcg tactgctcgt agtcggcatc ggtcgtgctg
     1381 tccggggtta tgtagttcgt gccgttgaac ttgatccccg atcggaggag gaagcggggg
     1441 ttgagccgga atccgaccga gcccttcaag tagccttcat tggtgctgta ggaggagtac
     1501 gcggcccgat accggcgcgc gctggtgttg ccctcaaggt agaaagtgag gcggccggcc
     1561 gggtcggtcg gcacccagat caagccggcg cgcggggtgt tgttgccgag gccgatgacg
     1621 ttattgtagt aggtataatc ggtgccgagg ctgagctgga gctgggggag ggggtaccac
     1681 ctggcggtga ggcgggctgt ggagtaagca tcgtcctggg ccgaggcctc gctcaggagg
     1741 ttgttggtgt aaccgttg
//
LOCUS       DAOOKS010000478         1756 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_86798_length_1756_cov_3.526749, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000478 DAOOKS010000000
VERSION     DAOOKS010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86798_length_1756_cov_3.526749"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1352
                     /locus_tag="PK112_07660"
     CDS             <1..1352
                     /locus_tag="PK112_07660"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPC11958.1"
                     /translation="GARFEFVAARDGSEGLQMVSTEQPELILLDIKMPGMSGIEVLEK
                     LNKLESRPEVIMVSGHGDTNYVVESIKLGAAEFINKPFDVQEIEIHINGVLERRNLRR
                     EVKELKSELQAQSAYANFLGDSVAMTKVKEIIEQVADSELTVLIRGESGTGKEIAARS
                     LHQLSARKDRPFIKVNCAAIPRDLLEAELFGYEKGAFTGAHKTKQGRFEIAHKGTMFL
                     DEIGDMPMELQSKLLQVLEQQEFVRVGGITSIHVDVRIICATNRNLEQAIHDKEFRDD
                     LFYRLNEITLFLPPLRERPEDIPLLVRHFIDKYNQLYSKDFRELSPSTIDRLMTFSWP
                     GNVRQLENMLKQVAVRGDESIIIDLINSAAAAPTMPSRSTYTPATTDVGPTNAAGTET
                     YSLKERIGRTVAEEEKRLISEVLTRTNWNRRKAADLLEISYRSLLYKIKDYNLNSSK"
BASE COUNT          411 a          575 c          431 g          339 t
ORIGIN      
        1 ttggggcccg ctttgagttt gtcgctgccc gcgacggctc cgaggggctc cagatggtct
       61 cgaccgagca acccgaactc attctcctgg atatcaagat gcccggaatg agcgggatcg
      121 aggtcctcga aaagctcaac aagctcgaga gccgccccga agtcatcatg gtctccggcc
      181 acggcgacac caactacgtc gtcgaatcca ttaagctggg cgccgccgag ttcatcaaca
      241 agccgttcga tgtccaggaa atcgaaatcc acatcaacgg cgtcctcgaa cgccgcaacc
      301 ttcgccgcga ggtcaaagag ctcaaatccg aactgcaggc gcagtcggcc tacgccaatt
      361 tcctcggcga ctccgtggcc atgaccaagg tgaaggagat catcgagcag gtcgccgact
      421 ccgagctgac tgtcctcatc cgcggcgagt cgggcaccgg caaagagatc gccgcccgct
      481 ccctgcacca gctctccgcg cgcaaagacc gcccgtttat caaggtcaac tgcgccgcca
      541 tccctcgcga cctgctcgag gccgagctct tcggctacga gaagggggcc ttcaccggcg
      601 cgcacaaaac caagcagggg cggttcgaaa tcgcccacaa gggaacgatg ttcctcgatg
      661 aaatcggcga catgcccatg gagctccagt ccaaactgct ccaggtcctc gaacagcagg
      721 agttcgtccg ggtcggcggc atcacctcga tccacgtcga tgtgcggatc atctgcgcca
      781 ccaaccgcaa cctcgaacag gcgatccacg acaaagaatt ccgcgacgac ctgttctacc
      841 gcctgaacga gatcaccctg ttcctgcccc ccctgcggga acgtcccgaa gatatccccc
      901 tgctcgtgcg gcactttatc gacaaataca accagctcta ttccaaagac ttcagagagc
      961 tctccccctc cactatcgac cgcctcatga ccttctcatg gcccggaaac gtccgccagc
     1021 tcgagaacat gctcaaacag gtggcggtgc gcggcgatga gtcgatcatt atcgacctga
     1081 tcaactccgc ggccgccgca ccgaccatgc cctcccgctc cacctacacc cctgccacca
     1141 ccgacgtcgg cccgactaac gccgccggga ccgagaccta ctcgctcaaa gaacggatcg
     1201 gccgcaccgt cgccgaggaa gaaaagcggt tgatcagcga agtcctcacc cgaaccaact
     1261 ggaatcgccg caaagccgcc gaccttctcg agataagcta ccgctcgctc ctctataaga
     1321 ttaaagacta caacttgaat tcttcgaagt agtcgttccg gcgatctgtc caaaaacgta
     1381 cccgatgccc gcctgcgtct tatatgcatc ggtgtgcata ttctgcacac atcctttctt
     1441 attttccaca tttttcactc catgtgaata acattaagtt gttgtgaaat aacgtgtaag
     1501 ttgttgaccg cgacaaatct cggcacaagt ttcggaaatg ttttccgcaa tccggcatcg
     1561 gggttgcatg gggaacaaaa cttgcttgta aaatagtgca cagaagcgcg atagcgacga
     1621 ataacgacgt ggtgcaacga tcatggatga ctgcaacttc attgacaagg tcgacctcga
     1681 gctcaaacga gccgagcgat attcggtctt cgtctcgctc gtcgttttcg acctctcatt
     1741 cctggagaac cactta
//
LOCUS       DAOOKS010000479         1755 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_86865_length_1755_cov_3.820000, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000479 DAOOKS010000000
VERSION     DAOOKS010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86865_length_1755_cov_3.820000"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1493
                     /gene="nadB"
                     /locus_tag="PK112_07665"
     CDS             <1..1493
                     /gene="nadB"
                     /locus_tag="PK112_07665"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HPC11959.1"
                     /translation="KRESDSSTNRAQGGIAAVLSGADSFESHVADTLEAGAGLCRRDV
                     VETIVREAPDAIRDLVEIGVRFTRTAEGYALGREGGHRAARVVHADDLTGQEIERALL
                     AAIKERRGQIDVYRDHIALDLIKGTADGTTVCAGAYVFSEKDHSFYAFYAPVTLLATG
                     GLGQVYFHTSNPKIATGDGVAMAYRAGVPVGNLEFIQFHPTILYSPGRWPFLISEAVR
                     GEGGVLKAVDGRLIMDGAHELKDLAPRDIVARAIDKELKQSGEEYVLLDISHRDAAFI
                     KKRFPNIYKQCLQRGFDLTRRPIPVVPAAHYACGGVVSRVSGETALAGLYVAGEVAMT
                     GMHGANRLASNSLLEAVVMAKRAAAAACAYAEAAPPAPHAAITPDFYSSLQYPREKVL
                     LAHDRSRLTRVMWDFVGIVRTENRLRLALEEVQRVKNAVEQYYLATPATYQVVELRNM
                     ATVAELIVRSALLRKESRGLHYIEDYPAPAPGAPTDTIITNPHAQG"
     gene            1500..>1755
                     /locus_tag="PK112_07670"
     CDS             1500..>1755
                     /locus_tag="PK112_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP synthase (glutamine-hydrolyzing)"
                     /protein_id="HPC11960.1"
                     /translation="MAEPHDMILILDFGSQYTQIIARRVREAHVYCEIVPYNHDLGAY
                     RGRNVRGYILSGGPASLSDPGSPRLAPEFYDTNLPVLGICY"
BASE COUNT          290 a          638 c          570 g          257 t
ORIGIN      
        1 agaagcggga gtcggactcc agcaccaacc gcgcccaggg cgggatcgcg gccgtcctct
       61 ccggcgccga ttccttcgag tcgcacgtcg ccgacaccct ggaggccggg gcgggcctgt
      121 gtcgccgcga cgtggtcgaa acgatcgtgc gggaggcccc cgacgcgatc cgcgacctcg
      181 tggagatcgg cgtgcggttc acgcggacgg ccgaggggta cgcgctcgga cgcgagggcg
      241 gacaccgggc ggcccgtgtg gttcacgccg acgacctcac cggacaggag atcgaacgcg
      301 ccctcctggc ggccatcaag gagcgccggg ggcagatcga cgtgtaccgc gaccacatcg
      361 ccctcgacct gatcaaggga acggccgacg gcacgaccgt gtgcgccggc gcctacgtgt
      421 tctccgagaa ggaccacagc ttctacgcct tctacgcgcc cgtcaccctg ctcgccacgg
      481 gcgggctcgg gcaggtctat ttccacacca gtaacccgaa aatcgcgacc ggcgacggcg
      541 tggcgatggc ctaccgggcc ggcgtgccgg tcgggaatct cgagttcatc cagtttcacc
      601 cgaccatcct ctacagcccc gggcgctggc cgttcctcat ttcggaagcg gtgcgcgggg
      661 aaggcggggt gctcaaagcg gtcgacgggc ggctgatcat ggacggcgcc cacgagctga
      721 aagacctcgc cccgcgcgat atcgtcgccc gcgccatcga taaggaactc aagcagagcg
      781 gcgaggagta tgtcctcctg gacatcagcc accgcgacgc ggcctttatc aagaagcgct
      841 ttccgaacat ctacaagcag tgcctccagc gcgggttcga cctcacccgg cggccgatcc
      901 cggtggtgcc ggcggcccac tacgcctgcg gcggggtggt ttcccgggtc agcggcgaga
      961 ccgcgctcgc cgggctgtac gtcgccgggg aagtggcgat gaccgggatg cacggcgcta
     1021 accggctggc ctccaactcg ctgctcgagg cggtggtgat ggccaagcgc gcggccgcgg
     1081 ccgcgtgcgc ctacgccgag gctgccccgc ccgcgcccca cgccgccatc acccccgact
     1141 tctactcctc cctccagtac ccgcgcgaga aggtgctgct cgcccacgac cgcagccgcc
     1201 tcacccgcgt catgtgggac ttcgtgggca tcgtgcgcac cgagaaccgc ctccgcctgg
     1261 cgctcgagga ggtccagcgg gtgaagaacg ccgttgagca gtactacctg gcgacgccgg
     1321 cgacctacca ggtcgtggaa ctgagaaaca tggcgaccgt ggcggaactg atcgtgcgct
     1381 cggccctcct gcgcaaggag tcgcgcgggc tgcactacat cgaggactac cccgctcccg
     1441 cccccggcgc gcccaccgat acgattatca ccaatcccca tgcgcaagga taggacaccg
     1501 tggcggaacc gcacgacatg atcctcattc tcgatttcgg ctcgcagtac acgcagatca
     1561 tcgcgcggcg cgtgcgcgaa gcgcacgtct attgcgagat cgttccctac aaccatgacc
     1621 tcggcgccta ccgcggccgc aacgtgcgcg gctacatcct ctcgggcggg ccggcctcgc
     1681 tgtccgatcc cggatcgccg cggctggccc cggaattcta cgacaccaac ctgcccgtgc
     1741 tcggcatctg ctacg
//
LOCUS       DAOOKS010000480         1755 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_86884_length_1755_cov_3.084706, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000480 DAOOKS010000000
VERSION     DAOOKS010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86884_length_1755_cov_3.084706"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1755
                     /locus_tag="PK112_07675"
     CDS             <1..>1755
                     /locus_tag="PK112_07675"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPC11961.1"
                     /translation="LSSAKAAGQLASDFAALDLRPRQLLSRRLNIWLYEYRADGHKDT
                     AAHEEVLARVRRHPAVELGQFNHEVALRSTFPDDSSFGQQWALHNTGQTGGVADADID
                     APEAWDLTTSGTTADGDQIVIAIVDRGYDLNHPDISFFKNTHETPANGIDDDGNGYID
                     DYDGWDAYSNDGTLPASDHGTHVAGIAAAIGNNGAGVSGVNWQVKVMPIAGSSTSEAT
                     VVAAYAYALEMRAQYNESEGALGAFVVATNSSFGVDYGDPVNFPVWCAMFDSLGAYGV
                     LSAAATANIGMDIDANGDVPTACPSDYLIAVTNTTSSDVRNSGAGWGAASIDLGAPGT
                     SVYSTLLGGGYGNKTGTSMASPHVAGAVGFMYSAASPVVIDLARRRPDSVALLIRQAI
                     LDGVDSIGSLTGVTVTGGRLNLRTAAVLVQAIPTGVTIVHQPLADTRDTVNDYAVAAQ
                     ITSDTTLAPDSLRLHYEIASVWTSAILSPAGGVQYSGAIPAQPPGTTIRYYLTAADVE
                     GKADTTDIFTFRVIDYAVSVRPDWQTQTGLVSDTMWFDLWVVNTGLRADDYTLGIGQA
                     KWVTQIFDSTMAGPISAT"
BASE COUNT          304 a          594 c          586 g          271 t
ORIGIN      
        1 aattgtcctc ggccaaagcc gccggccaac tggcttcgga tttcgccgcc ctcgacctgc
       61 ggccccggca actgctctcg cgccgcctga acatctggct ctacgaatac cgcgccgacg
      121 ggcacaagga cacggctgcg catgaggagg tgctcgcccg ggtgcggcgg catcccgcgg
      181 tcgagctcgg ccagttcaat catgaagtgg ccctccgctc gaccttcccc gacgactcct
      241 cgttcggcca gcagtgggcg ctgcacaaca ccggccagac gggaggggtt gcggatgccg
      301 atattgacgc tcccgaggcc tgggatctga cgacctccgg gacgacggcc gacggggatc
      361 agattgtgat cgccattgtc gaccgcgggt acgacctgaa ccaccccgac atctcctttt
      421 tcaagaacac ccatgaaacg cccgcgaacg ggattgacga cgacggcaac gggtatatcg
      481 acgactacga cggctgggat gcgtactcga acgacgggac gctgccggcg agcgaccacg
      541 gaacgcacgt ggcggggatc gccgcggcca tcggcaacaa cggcgcgggg gtgagcgggg
      601 tcaactggca ggtgaaggtg atgccgatcg ccgggtcctc gacttccgag gcgacggtgg
      661 tggccgcgta cgcctacgcc ctggagatgc gggcgcagta caacgaatcg gagggcgcgc
      721 tcggagcgtt cgtggtggcg accaattcct cgttcggcgt ggactacggc gacccggtca
      781 atttccctgt ctggtgcgcc atgtttgact cgctcggggc ctacggcgtg ttgagcgcgg
      841 cggccaccgc caacatcggc atggacatcg acgcaaacgg cgacgtcccc accgcctgcc
      901 ccagcgacta cctgattgcg gtgaccaaca caacctccag cgacgtgcgc aacagcgggg
      961 ccggctgggg cgccgcctcg atcgacctgg gcgcacccgg gacctcggtc tactccacgc
     1021 tgctcggagg cgggtacggg aacaagacgg ggacgtcgat ggcctccccc catgtcgcgg
     1081 gggcagtcgg gttcatgtac agcgcagcct cccccgtggt catcgacctg gcgcggcggc
     1141 gccccgactc ggtagccctg cttatccgcc aggctatctt ggacggcgtc gactcgatcg
     1201 ggtcgctcac cggcgtgacg gtcacgggcg gacggctcaa ccttcgcacc gcggcggtgc
     1261 tggtgcaggc catcccgacc ggcgtgacga tcgtccacca accgctcgcg gacacccggg
     1321 ataccgtgaa cgattacgcg gtcgccgccc agattacgtc ggacacgact ctggcgccgg
     1381 attcgctgcg cctgcactac gagattgcga gcgtgtggac aagcgcgatc ctttccccgg
     1441 ccggcggagt ccagtacagc ggcgccattc ccgcccagcc gcccggcacg acgatccgct
     1501 actatctgac cgcggcggac gtggagggaa aagcggacac caccgacatc ttcacgttcc
     1561 gggtgatcga ctacgcggtg agtgtgcgcc ccgactggca gacgcagacg gggctggtgt
     1621 ccgacacgat gtggtttgac ctctgggtgg tcaacacggg gctgcgcgcg gacgactaca
     1681 ccctggggat cggacaggcg aagtgggtga cccagatttt cgacagcacc atggccggcc
     1741 cgatcagcgc gaccg
//
LOCUS       DAOOKS010000481         1751 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_87215_length_1751_cov_2.626179, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000481 DAOOKS010000000
VERSION     DAOOKS010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87215_length_1751_cov_2.626179"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1410)
                     /locus_tag="PK112_07680"
     CDS             complement(<1..1410)
                     /locus_tag="PK112_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11962.1"
                     /translation="MYLPTVSLFVRRLLPVALLVGALGTAVWAGPAVIRVESIENAAL
                     ADTVELAVIMDAPHDSSALTGFNLLLTYDQTGMAFLAADAGQLLVDCQWEYFTYRSGI
                     DSTLGGSPLALVRLVAIAEINNGSVHPDCPLDTPGELARLRFRTTASPGAADHQFPVR
                     FYWHDCDDNVFADSTGDTLLISNRVFEFGQEITEEHPFPTYFGAPSACLIQSTIIRGA
                     DFYNGSVRIDAQAAPPDAPVRVVISPLEAPVLGGPVEFAAQLHQVQSGLALGGFDFLV
                     RYDPAQLTWMSAQPGNLLESCGWEYFTFAAGAQAECGQFPCPAGVARVIAVADLASPT
                     GTPTCGADTEGTLANFLFTVANDTSLIGRPLAVEWYWYTCDDNRMAVLSGDTTFISRS
                     VYDHFGQPAPADTAFPTASGAPSLCAAGPDIIPGIDFFAGWTRAHPSEIDLRGDVNLN
                     GIANEVSDYVLFGNYFFYGL"
BASE COUNT          319 a          498 c          628 g          306 t
ORIGIN      
        1 caggccgtag aagaagtagt ttccgaagag cacgtagtcg gacacctcgt tggcgatccc
       61 gttcaggttg acgtcgccgc gcaggtcaat ttcggaaggg tgggcgcggg tccagccagc
      121 gaagaagtcg atgcccggga tgatgtcggg gccggcggcg cagagcgacg gggcgccgga
      181 cgcggtcggg aaagcggtgt cggccggcgc cggctggccg aaatggtcat acacggatct
      241 cgagataaaa gtcgtgtcgc ccgagagaac ggccatgcgg ttgtcgtcgc aggtgtacca
      301 gtaccactcc accgcaaggg ggcggccgat gagcgaggtg tcgttcgcca cggtgaagag
      361 gaaattggcg agggtgcctt cggtatcggc tccgcaggtc ggggttccgg tcggggaggc
      421 caggtcggcg acggcgatca cgcgcgccac gcccgccggg caggggaatt gcccgcactc
      481 ggcctgggcg ccggctgcga aggtgaagta ctcccacccg caggactcca gcaggttgcc
      541 gggctgggcc gacatccagg tgagttgcgc cgggtcatac cggacgagga agtcaaagcc
      601 gccgagcgcg agacccgact gcacctggtg cagttgggct gcaaattcga ccggaccgcc
      661 cagcacgggg gcctcgagcg ggcttatcac taccctcacc ggagcgtcgg ggggcgccgc
      721 ctgcgcatcg atccggacgc tgccgttgta gaagtctgcc ccgcggatga tcgtgctctg
      781 aatcaggcag gcggacggcg cgccgaaata agttgggaac ggatgctcct cggttatctc
      841 ctggccgaat tcgaagacgc ggtttgagat caggagggta tcgccggtgg agtcggcgaa
      901 tacgttgtcg tcgcagtcgt gccagtagaa tcggaccggg aactgatggt cggcggctcc
      961 gggcgaagcg gttgtgcgga aacgaagccg cgccagctcg ccgggtgtat caagggggca
     1021 gtccgggtgg accgatccgt tgttgatctc cgcgatcgcg acgagccgga ccagggcgag
     1081 gggcgaaccg ccgagcgtcg agtcgatccc cgagcggtag gtgaagtact cccactggca
     1141 gtcgacgagc aactggcccg cgtcggcggc gagaaaggcc atcccggtct ggtcgtacgt
     1201 gagcaagagg ttgaacccgg tcagagcgga cgaatcatgg ggggcatcca tgatcaccgc
     1261 gagctcgacg gtatcggcga gggcggcatt ttcgatgctc tcgacgcgga tcaccgccgg
     1321 cccggcccag acggccgttc ccagggcgcc gaccagcaac gcgaccggca gcaacctgcg
     1381 gacaaacaag gagacggttg gcaggtacat acaaacctcc gcaacaagtt ttgtggtgcg
     1441 ctgattcggt ccgttaggtc tcggtcgata gcgtaacgtc cgcgtcgggc gccggctcgg
     1501 gccggtcgcc gttcggcgcc gcgagaattg ttcggtcgat tattcggtcg gtagcgaagc
     1561 gggctatagg ctatccagtc tgtaccagat gcgatgaacg tatctttgca tatctcgcct
     1621 gagtatacgt attccgacag tctctgtcaa ggtctttttt gccggcttcg ggcgggcggt
     1681 tgtgattgcc gaggttgcgt gcgtggcgta tactgctgtg acgatggatt tcttcgacca
     1741 taccggtcaa t
//
LOCUS       DAOOKS010000482         1749 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_87346_length_1749_cov_3.353011, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000482 DAOOKS010000000
VERSION     DAOOKS010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87346_length_1749_cov_3.353011"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..>1749
                     /locus_tag="PK112_07685"
     CDS             39..>1749
                     /locus_tag="PK112_07685"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HPC11963.1"
                     /translation="MHRTRTERIRLGILFSLVCLYFLAVAARLVHLQVVLHQKYADIV
                     RIQSEGRVKIPAERGLIYDRYGRLVVNNVKCPSLYAYPGSQTELENAARYVEKMFGLK
                     RGTARREYNLEVNKFSWIKRLLGDEFARHIEKTAPPGLYLRDETQRVYPYGLVGKQIL
                     GFTNIDNQGQSGLELAWDSLMKGEDGEADIRRDGLRNVFRVKEQAAVKPKPGQALVLT
                     IDFNLQEIFERQLRKGVEEYGAKAGMGAFIDCRTGEILAMAHYDPKEEHPEKPVKLRA
                     ISDQFEPGSSFKAITAAALLEAGEVDFREYTYCEMGAWKMERGGTLKDDKKHGNLTFR
                     EIMELSSNIGVAKYGIRLGGENLYEAARKFGFGQKYGIGLGAEESGRVSKPRRWADRA
                     VASLAMGHAVAVNCLQMANAFAAIANGGELLRPHLLMCCVDEHQKVTRRFGREVMQKV
                     MEGVWADTLKAVLRGVVERGTATKVNSKAVAIAGKTGTAQIPDSVTHRYKDEYTASFA
                     GFFPYEKPAIAGIVMLIEPNPIHYGGYTAGPIFRRTAEQYMILNPDLFDSGTGIYANP
                     QGDG"
BASE COUNT          369 a          528 c          587 g          265 t
ORIGIN      
        1 ccggtcatcg actccctggc caacggaggg gacgactgat gcatcggacg cgcaccgaac
       61 gaatccgact cggcatcctc ttctcgctcg tctgcctgta tttcctggcg gtcgcggcgc
      121 gcctggtcca cctccaggtg gtcctccacc agaagtacgc cgacatcgtg cgcatccagt
      181 cggagggacg ggtgaaaata ccggccgagc ggggcctcat ttacgaccgg tacgggcgcc
      241 tggtcgtgaa caacgtcaaa tgcccgtcgc tctatgccta ccccgggtcg caaacggagc
      301 tggagaacgc cgcccgctat gtcgagaaaa tgttcggcct gaagcgggga acagcgcgca
      361 gagagtacaa cctcgaggtg aacaagttca gctggatcaa gcgcctgctc ggcgacgagt
      421 tcgcccggca catcgaaaaa acggcgccgc ccgggctgta cctgcgcgat gagacgcagc
      481 gggtgtatcc ctacggactg gtcggcaagc agattctggg tttcacgaat atcgacaacc
      541 aggggcagtc cgggctggag ctggcgtggg acagcctcat gaagggggag gacggggagg
      601 cggatatccg gcgggacggt ctgcgcaacg tcttccgcgt caaagagcag gcggccgtga
      661 aacccaaacc ggggcaagcg ctcgtcctca ctatcgattt caacctccag gaaatcttcg
      721 aacggcagct ccgcaagggg gtcgaggagt acggggcgaa ggcggggatg ggggcgttca
      781 tcgactgccg caccggtgag attctggcca tggcccacta cgatccgaaa gaagagcacc
      841 cggagaagcc ggtcaagctg cgggcgatca gcgaccagtt cgaaccgggt tcgtcgttca
      901 aagcgatcac ggcggcggcc ctgctggagg cgggggaggt ggacttccgc gagtacacgt
      961 actgcgaaat gggggcgtgg aaaatggaac ggggcgggac gctcaaggac gacaaaaaac
     1021 acggcaacct cacgttccgc gagatcatgg agctctcgag caacatcgga gtggccaaat
     1081 acggaatccg cctgggcggc gagaatctct acgaggcggc gcggaagttc gggttcggac
     1141 agaaatacgg catcgggctc ggggcggaag agagcgggcg tgtttccaag ccccggcgct
     1201 gggcggaccg ggccgtggcc tcgctggcca tggggcacgc ggtggcggtg aactgcctgc
     1261 aaatggccaa tgccttcgcc gccatcgcca acggcggcga actcctccgc ccccacctgc
     1321 tcatgtgctg cgtcgacgaa caccagaaag tgacccgccg gttcggacgg gaagtaatgc
     1381 agaaggtgat ggagggagtc tgggccgata ccctgaaagc ggtcctgcgc ggcgttgtag
     1441 agcgggggac agcgacgaag gtgaattcga aagctgtcgc catcgccggc aagacgggta
     1501 ccgcccagat tcccgactct gtcacccacc gctacaaaga cgaatacacg gccagcttcg
     1561 cgggcttctt cccgtacgag aagccggcca tcgccgggat cgtcatgctc atcgagccga
     1621 acccgatcca ctacggcggc tacaccgcgg ggccgatatt ccggcggacg gccgagcagt
     1681 acatgatcct caaccccgac ctgttcgact ccggcacggg gatctacgcg aatccgcagg
     1741 gcgacggta
//
LOCUS       DAOOKS010000483         1747 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_87482_length_1747_cov_3.357565, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000483 DAOOKS010000000
VERSION     DAOOKS010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87482_length_1747_cov_3.357565"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="PK112_07690"
     CDS             complement(<1..401)
                     /locus_tag="PK112_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11964.1"
                     /translation="MVSGVFLRAPPPLLSTAPAGDVLVAAPTRPSHHPRMHYRLLARL
                     DSAVSPVRPALLPYCRLRLGEVWCDPEGRHTVACGDAADRAFVRRLFGARRATLAVQD
                     PPYNLVMGQKLPPEAYANWCRAWVDNTDAILA"
     gene            487..987
                     /locus_tag="PK112_07695"
     CDS             487..987
                     /locus_tag="PK112_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018193221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HPC11965.1"
                     /translation="MEDNAIWAPWRAGFVLGKKEDGCVFCNRIAHDRDSVENLIVFRA
                     RLNVVILNKYPYNPGHALIVPIRHLGGLDELTVDEANEFFQLTRDAVRLMERAYQPQS
                     FNLGMNLGRESGAGIPGHVHMHIVPRWVGDTNFMPVIGRTKVQSVPLDAVYERLRNEF
                     VEEFGS"
     gene            987..>1747
                     /locus_tag="PK112_07700"
     CDS             987..>1747
                     /locus_tag="PK112_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11966.1"
                     /translation="MARRRKIPTKGELLQLQKLYKTDEKIGERLGGVPAYLVAYWRRK
                     KNVAKHSVPKFSEAEVRNLWERFGDDDRCGLELGISKAAFYNWRRRYGIREKPPFLKL
                     EQLELNLPGLKQRGHAVSLLGKQSAAQKIIAMAAEREKVEVGELVVVQPDVTIAHQDA
                     AAVVRARLGGRAPQVGLILGSGLGGFADRLADPVRIDYGELPGFPVSKVHGHAGRLVV
                     GTRGGATCVAMQGRVHLYEGHRAVDVAFPARVLVAL"
BASE COUNT          305 a          528 c          632 g          282 t
ORIGIN      
        1 gccaggatcg catcggtgtt gtcgacccag gcacggcacc agttcgcgta ggcctcgggc
       61 ggcagtttct gtcccatgac gaggttgtac ggcgggtcct gcaccgccag cgtcgcgcgg
      121 cgcgcgccga agaggcggcg cacaaaggcg cggtcggccg catcgccgca ggccaccgta
      181 tgccgtccct ccgggtcgca ccagacctct cccaggcgca aacggcagta cggcagcagc
      241 gccgggcgca ccgggctgac tgccgaatcg aggcgggcca gcagacggta atgcatgcgg
      301 ggatgatggg agggtcgggt gggagcggca actaaaacat cgcccgccgg cgcggtcgat
      361 aacagcgggg gcggagcgcg gagaaagacg ccgctaacca tctaaacgac agcagcttgt
      421 gaaaatgttg atattccccg gggcggggct tggcagtgtg ggtaattttg tttattctac
      481 accatcatgg aagacaatgc gatatgggcc ccctggcgcg ccgggttcgt gctcggcaag
      541 aaagaggacg gttgcgtctt ctgtaaccgc atcgcgcacg accgcgactc ggtcgagaac
      601 ctcatcgtct tccgcgcccg actcaatgtc gtcatcctca acaagtatcc ctacaatccc
      661 ggccatgcgc tgatcgtccc gatccgccac ctgggcgggc tcgacgagct gacggtcgac
      721 gaggccaacg agtttttcca gttgacgcgc gacgcggtgc gcctgatgga gcgggcgtac
      781 caaccgcagt cgttcaattt gggaatgaat ctcgggcggg aatcgggggc gggaatccca
      841 gggcacgttc acatgcacat tgtcccgcgg tgggtggggg acacgaattt catgccggtg
      901 atcggccgga ccaaagtgca gtcggtgccg ctcgacgccg tgtacgagcg gcttcggaac
      961 gaatttgtcg aggagttcgg aagctgatgg cgcggcggcg caagatcccg accaaggggg
     1021 agcttctgca gctgcagaag ctgtacaaga ccgacgagaa gatcggcgag cgtctcggcg
     1081 gcgtgcccgc ctacctcgtg gcctactggc ggcggaagaa gaatgtcgcc aagcattcgg
     1141 tgccgaaatt ttcagaggcg gaggtccgga acctgtggga gcggttcggg gatgacgacc
     1201 ggtgcgggct ggagctgggg atatcgaagg cggcgttcta caactggcgg cggcggtacg
     1261 ggattcggga gaagccgccg tttctgaagc tcgagcagct cgaactcaac cttcccggcc
     1321 tcaagcaacg cggccacgcg gtcagtttgc tcggcaagca gtcggcggcg cagaagatca
     1381 tcgccatggc ggcggagcgg gagaaagtgg aggtcggcga actggtcgtg gtgcagccgg
     1441 atgtgacgat cgcgcaccag gacgccgcgg cggtggtgcg cgcccgcctg ggcggtcgcg
     1501 cgccccaggt cgggctcatc ctcggctcgg gcctcggcgg gttcgccgat cggctggccg
     1561 atccggtgcg catcgactac ggcgagctgc ccggcttccc ggtgtcgaag gtccacggcc
     1621 acgccggccg gctggtcgtc ggcacgcgcg gcggcgcgac ctgcgtcgcg atgcagggcc
     1681 gcgtgcactt gtacgagggg caccgcgccg tcgacgtggc gttcccggcc cgggtgctgg
     1741 tggcgct
//
LOCUS       DAOOKS010000484         1739 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_88086_length_1739_cov_2.997625, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000484 DAOOKS010000000
VERSION     DAOOKS010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88086_length_1739_cov_2.997625"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="PK112_07705"
     CDS             complement(<1..194)
                     /locus_tag="PK112_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPC11967.1"
                     /translation="MDVIEQLGSLALATRLRRLADRLQRDVSQVYRELNVEFEARWFA
                     VVYYLKDHNEVREAHQERAAT"
     gene            complement(453..1385)
                     /locus_tag="PK112_07710"
     CDS             complement(453..1385)
                     /locus_tag="PK112_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11968.1"
                     /translation="MRGNSQIAGALAAVVTMAAAVVSLSWTAAAVAEEGAGSAPSFQE
                     VRLAASDVTPIEKDGCPTVYYYDEESTAYVVWRLPQEGVEEFGERFDQEGVSVCTLVT
                     VRALLYGPQMAGSPDVEVVVYQDDGTGLPGAQLAAVTVPNASLPTTGLGWIEADFSPS
                     SLVFSEGETYHLTLRVSPAVAGDTVSLCSDEATGPHSGENRSEYFDGDAWWTLSSAVG
                     TDYVFVFEAEIDFGCDTTCCVGGAGNVNGDPVDKVDVADLTFPVSYLFRGGVEPPCRE
                     EANVNGDPDGDVSVSDLTLLVGYLFRGGPAPAPC"
BASE COUNT          333 a          612 c          464 g          330 t
ORIGIN      
        1 gtggcggcgc gttcctgatg ggcctcacgg acctcgttgt ggtccttcag gtagtacacg
       61 accgcaaacc agcgcgcctc aaactcgaca ttgagctccc ggtacacctg tgacacatcc
      121 ctctggaggc ggtccgccag tcggcgcagc cgggtcgcca acgccagcga gccgagttgt
      181 tcgatcacgt ccattgtctt cgggctccct ctatcatccg agtgtacgta agtgcttaca
      241 taattgtttc gcggatgcgt gcgatgacca tcgcaccgac ctgccttcag gatggctcaa
      301 tctaccactc cccgtttcgc actgcaataa ggaaaaaggt tctttcgatc gcccacccca
      361 ccggcttgag ataggaaggg ccgatcgtcc gattgcgggc ggccgcccgt ctctttcgac
      421 ttccgaagct gtgtctccgc gcggtccggg gtctagcacg gcgccggcgc cggccccccg
      481 cggaagaggt acccgacgag aagcgttagg tccgaaacac tcacatcacc gtcgggatcg
      541 ccgttgacgt tggcctcttc ccgacacggc ggctccactc ccccgcggaa caggtacgac
      601 acggggaatg tcagatccgc cacgtcgact ttgtcgacgg gatccccgtt cacattcccg
      661 gcgccgccga cgcagcaggt agtatcgcag ccgaaatcta tctccgcttc aaatacgaac
      721 acgtagtccg ttccaacggc cgagctgagc gtccaccacg cgtcgccgtc gaagtactct
      781 gaccggttct ctcccgagtg aggaccggtc gcctcatcgc tgcacagcga tacggtatct
      841 ccggctaccg ccggcgaaac gcgcagggtg aggtggtagg tctctccctc gctgaaaacc
      901 aggctgctcg gcgaaaaatc ggcctcgatc cagcccaggc cggtggtggg cagggaggcg
      961 ttcggaacgg tgaccgcggc cagctgagcg cccggcagcc ccgtaccgtc atcctgatac
     1021 accaccacct ccacatccgg cgagcccgcc atctgtggcc catacagcag cgcccggacc
     1081 gtcacaagag tacagaccga gaccccctcc tgatcgaatc gctcgccgaa ctcctcgacc
     1141 ccctcttgcg gcaacctcca gacgacatac gccgttgatt cctcgtcgta atagtagacc
     1201 gtaggacaac catccttctc gatcggcgtc acatcacttg ccgccagccg cacctcctga
     1261 aaacttggag ccgaacctgc cccttcctca gcgacagccg ccgctgtcca cgacagactc
     1321 accaccgcgg ccgccatggt cacgacagcc gcaagagccc ccgcaatctg cgagttaccc
     1381 ctcacatgac acctcgtctc gttgagaacc gcttcggacc gacacttcgt cacccgctcg
     1441 ggcaacgtcc gtcgaacgtc cgcctcatcg atcttcccgg actccaggtg taacgatgaa
     1501 cttccctgcg tcgtctgaag gggccaactg tttccgacca acgtcggctt tgccgccgat
     1561 catgttccgg ccgggagacg accctgcccg agatgtcatt cggagtcccg gctgaaggca
     1621 gttgtcacac gatgttgagt ttcaacgagg gtctgctgca gcgataaagc tgtctcgatt
     1681 tgccttgctt gacgatatcg cggcacatca ccgtaccgaa ctccttctca aaggccttg
//
LOCUS       DAOOKS010000485         1721 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_89490_length_1721_cov_3.978391, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000485 DAOOKS010000000
VERSION     DAOOKS010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89490_length_1721_cov_3.978391"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(142..759)
                     /locus_tag="PK112_07715"
     CDS             complement(142..759)
                     /locus_tag="PK112_07715"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HPC11969.1"
                     /translation="MISLLVISGSPVPGASTDILLRQAADAVRRGLRGAARVRTTFVK
                     LNDLRILPCQACGEAPTPQFCFFDDDMAPLYRELARCDCLLFGSPIHFDTVSSQAKLF
                     IDRCNCVRPADFRGADPDHGFLKLIARKRPGAMVLVSGERQWTEGARRVIAGFFKWAE
                     VTNEGLLTYRTRGLEAGEAAGSAAALAEADALGHRLAGLLRRRCG"
     gene            complement(752..>1721)
                     /locus_tag="PK112_07720"
     CDS             complement(752..>1721)
                     /locus_tag="PK112_07720"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPC11970.1"
                     /translation="IAVRSSGRSFAHVLEVRSVTLAYGAEPVLRNVSFDLYRGEKIGL
                     VGRNGSGKSTLLKALIGELAPVSGELRLGANVDVVYFDQDLDNLRLEATVLDNIWEED
                     PMAEVGAMRSFLARFGFTGEDCFKRVSALSGGERTKLSLARIIYHPANFLIFDEPTNH
                     LDIDSREALEEALGRFDGSCLIVSHDRHFLDRVADRIIHLAGGTAFVYGGGYSHFEEK
                     QAEAAAAALEAAPRPDKEKSREVYQAFKEASRVKARLKKALKSTRSKIADHERELEEL
                     EDGIARRIPRTDWEALEAASRRKQEVEAALLALYGELEELERRDCD"
BASE COUNT          277 a          630 c          533 g          281 t
ORIGIN      
        1 accaccacct cgcggccgtc ggccagtccg cgcaggaaag cggcctcgtc gtcgatctct
       61 tttcggcgcg ggggattgtc gcggtagtag atctgttcgt actccggtgc gcgtcgccgg
      121 tagtaccgcc gcgtctgctc gtcacccaca gcgcctccgg agcaatccgg ccagcctgtg
      181 tccgagcgcg tcggcctcgg ccagggccgc cgccgaaccg gccgcctcgc ccgcctcgag
      241 cccccgggtg cggtaggtga gcagcccctc gttggtcacc tcggcccatt tgaagaaccc
      301 cgcgatcacg cgccgggctc cctcggtcca ctgccgctcc ccgctcacca gcaccatcgc
      361 ccccggccgc ttgcgcgcga tgagcttgag gaaaccgtgg tcgggatcgg ccccgcgaaa
      421 atccgccggc cgcacacagt tgcatcggtc gatgaacagc ttcgcctggc tcgacaccgt
      481 gtcgaaatga atcgggctcc cgaacagcag gcagtcgcag cgcgccagct cgcggtagag
      541 cggcgccatg tcgtcatcaa aaaaacaaaa ctggggtgtc ggcgcctcgc cgcacgcctg
      601 gcaagggagg atgcgaagat cgttgagctt gacaaaggtc gtgcgcaccc gcgccgcgcc
      661 gcgcagaccg cggcggactg cgtccgccgc ctgccgcaga aggatgtcgg tcgaggctcc
      721 cggcacgggc gagcccgata taaccagcag actaatcaca gtcgcgccgc tccagttcct
      781 caagttcgcc gtacagcgcc aggagcgccg cttccacctc ctgcttgcgc cgcgaagcgg
      841 cctcgagcgc ttcccaatcg gtgcgcggaa tgcggcgggc gatcccgtcc tcgagttcct
      901 ccagctcccg ctcgtgatcg gcgatctttg agcgcgtcga cttcaacgcc ttcttcaggc
      961 gcgccttgac ccgcgaggct tccttgaacg cctggtagac ctcccgcgat ttttccttgt
     1021 ccggcctcgg cgcggcctcg agcgcggccg cggccgcttc cgcctgcttc tcttcgaaat
     1081 ggctgtagcc gccgccgtag acgaaagcgg tcccgccggc caggtggatg atccggtcgg
     1141 ccacccggtc gaggaagtgc cggtcgtggc tgacaatgag gcagctcccg tcgaaccgcc
     1201 cgagcgcttc ctcgagcgcc tcgcgcgagt cgatgtcgag gtggttggtc ggctcgtcga
     1261 aaatcaggaa attggccggg tggtagatga tccgcgccag cgacagtttc gtccgctccc
     1321 cccccgacag cgcggacacg cgcttgaagc agtcctcccc ggtgaatccg aaacgggcga
     1381 gaaacgaccg catcgccccg acctcggcca tcggatcctc ctcccagatg ttgtcgagca
     1441 ccgtggcctc gagccggagg ttgtccaggt cctggtcgaa atagacgacg tcgacattcg
     1501 ccccgaggcg gagctcgccg ctcaccgggg cgagctcgcc gatcagcgct ttgagcagcg
     1561 tcgacttgcc cgatccgttc cggccgacca gcccgatctt ctccccccgg tagagatcga
     1621 aactgacatt ccgcaggacc ggctccgcgc cgtaggccag cgtcaccgac cgcacctcga
     1681 gcacgtgggc aaacgaccgt cccgaggacc tcaccgcgat a
//
LOCUS       DAOOKS010000486         1720 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_89597_length_1720_cov_2.860661, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000486 DAOOKS010000000
VERSION     DAOOKS010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89597_length_1720_cov_2.860661"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="PK112_07725"
     CDS             <1..719
                     /locus_tag="PK112_07725"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481823.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 1 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HPC11971.1"
                     /translation="NLNANMGIFRPGDVGFDIMHFNLHKTFSTPHGGGGPGAGAVGVT
                     AELDPFLPFPVLEKADDGTLFMNYNRPHSIGRVHAFYGNFANQVRAYAYIKTLGGRGL
                     RRASENAVLNANYLKALLKDAFDLPYDVHCMHEFVLSGNRQKKKGVRTADMSKRMLDF
                     GVHSPTNYFPLIVPEALMIEPTETESRETLDRFAAIMRQIAREADTDPELVTSAPHHT
                     PVRRLDETAAARTLDVCFAE"
     gene            793..>1720
                     /locus_tag="PK112_07730"
     CDS             793..>1720
                     /locus_tag="PK112_07730"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPC11972.1"
                     /translation="MTYRYGAGAPAALANVGALIQPGESVAVMGANGSGKSTFARLAA
                     GLFAPTEGRVTVRNQRGEDTRAGILFQNPDNQMVAVTVEKEIAFALENAAVPPDVMAR
                     RVTQTLKRFSIAHLRQRLTAELSDGEKQRVALAAVMVSEPTVLVLDEPDSFLDEAGKA
                     MLRTELENLRRSQPSMIQIHITQYPQTARQYPRLMVFFEGRVAADGRPERIFADDAFC
                     RETAIAFDPRASDGEGSGAASIAAKDRRVARVELKEAAFAYAAIGPVLPPLNATVQSG
                     EVLGVVGPSGSGKSTLGLLLCRVLEPTSGRILY"
BASE COUNT          298 a          550 c          597 g          275 t
ORIGIN      
        1 cgaaccttaa cgccaacatg ggcatcttcc gccccggcga cgtcggcttc gacatcatgc
       61 acttcaacct gcacaagacg ttctcgacgc cgcacggcgg cggcgggccg ggcgccggag
      121 cggtcggggt caccgcggaa ctcgacccgt tcctcccctt cccggtgctg gaaaaggcgg
      181 acgacgggac gctgttcatg aactacaacc gcccgcactc gatcgggcgg gtgcacgcgt
      241 tctacgggaa tttcgccaac caggtgcggg cctatgccta catcaagacg ctcggcggcc
      301 ggggactgcg gcgcgccagc gagaacgcgg tgctcaacgc caactacctc aaggcgctgc
      361 tgaaggatgc tttcgatctg ccctacgatg tccactgcat gcacgaattc gtgctgtcgg
      421 gaaaccggca gaagaagaaa ggggtgcgga cagcggacat gtcgaagcgg atgctcgatt
      481 tcggcgtgca ctcgccgacc aactatttcc cgctcatcgt gccggaggcg ctcatgatcg
      541 agccgacgga gacggagagc agggagacgc tcgaccggtt tgcggcaatc atgcgccaaa
      601 tcgcgcgcga ggcggacacc gaccccgagc tcgtgacttc ggcgccgcac cacaccccgg
      661 tgcgccggct ggacgagacg gcggcggcgc ggacgcttga tgtctgtttt gcggagtgaa
      721 cgattccgct tgtgaatggg agccggtgcc tgttctttgg gcatcggctt ttttatggtt
      781 cgtttcgaag gtgtgacata tcgctacggc gcgggggcgc cggcggccct cgccaacgtc
      841 ggggcgctca ttcagcccgg cgagtcggtg gccgtcatgg gggccaacgg gtcggggaaa
      901 tcgaccttcg cccgcctcgc cgcgggcctg tttgcgccga ccgagggacg ggtgacggtg
      961 cggaaccagc gcggggaaga caccagggcg gggatcctct ttcagaatcc cgacaaccag
     1021 atggtggcgg tgacggtcga gaaggagatc gccttcgccc tcgaaaacgc ggccgtgccg
     1081 cccgacgtca tggcccggcg ggtgacccag acgctcaagc gcttctcgat cgcccatctc
     1141 cggcagcgcc tgaccgcgga gctgtcggac ggtgaaaagc agcgggtggc cctggcggcg
     1201 gtcatggtga gcgagccgac tgtgctcgtg ctcgacgagc cggattcgtt tctcgacgaa
     1261 gcggggaaag cgatgctgcg gaccgagctg gagaacctgc ggcggagcca gccctcgatg
     1321 atccagattc acatcacgca gtatccgcag acggcgcggc agtacccgcg cctgatggtc
     1381 ttcttcgagg gccgcgtggc ggccgacggc cggccggagc gaatcttcgc cgacgatgcc
     1441 ttctgccgcg aaacggccat cgccttcgac ccgcgcgcca gcgacggaga gggcagcggc
     1501 gcggcgagta tcgcagcgaa ggaccgccgg gtggcgcgcg tcgaactcaa ggaggcggcg
     1561 ttcgcctatg ccgcgatcgg accggtgctg ccgccgctca acgcgaccgt ccagtccggc
     1621 gaggtgctcg gggtcgtcgg accctcggga tcgggaaaga gcacgctcgg gcttcttttg
     1681 tgccgggtgc tggaaccgac ctcaggaagg atcctctaca
//
LOCUS       DAOOKS010000487         1718 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_89771_length_1718_cov_2.643416, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000487 DAOOKS010000000
VERSION     DAOOKS010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89771_length_1718_cov_2.643416"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="PK112_07735"
     CDS             complement(<1..537)
                     /locus_tag="PK112_07735"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HPC11973.1"
                     /translation="MRKKDIEPEDPVAPGGQPCESVERSGSGEAPPAAGVENGDAEPG
                     PPPGEIPEEEQLRRRIAELEDRLLRTAADFDNYRKRQARQYDELVKTAADRVLSDLLD
                     IVDNFRRALDAAAGEGNMDAYRQGTELIYGQMTELLARYDVTPIEAVGRPFDPNVHEA
                     LLRVPSAEHPAGTVAVEVS"
     gene            complement(530..1588)
                     /gene="hrcA"
                     /locus_tag="PK112_07740"
     CDS             complement(530..1588)
                     /gene="hrcA"
                     /locus_tag="PK112_07740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HPC11974.1"
                     /translation="MAFENLTEREKQVLSNLIEHYIRSADPVGSRVIAHKFRMGVSSA
                     TIRNTLQDLEELGLVRQPHTSAGRIPTDLGYRVYVDYLLKPEDLTESEKERIKDVIVS
                     HGRGLDDILGQTCRVLSDITNQLGVSIAPRFDRGVLRRIELISLSQGRIMAVVVVESG
                     LARSVIVEVETSIDEASLREVEQVLNERLAGLSLGEIRTTISKRLADHTGSGRLVKLI
                     IDSRDRIWADDPAGAIHLAGVERLLSQPEFSEIDRITRIVRLLEDNRTLKEFLSSAYE
                     EGLFITIGRENKFDEILNCSLVTSSYKVGSIAGTIGIIGPTRMEYGKLVSIVDYTARA
                     ITEVLSGMSSKEGESKDA"
BASE COUNT          285 a          609 c          484 g          340 t
ORIGIN      
        1 gctcacttcc accgcgacgg tccccgccgg gtgctcggcc gacggcaccc gcaggagcgc
       61 ctcgtgcacg ttggggtcga acggccgccc caccgcctcg atcggtgtga cgtcgtagcg
      121 cgccaacagc tcggtcatct gaccgtagat cagttccgtg ccctgccggt aggcgtccat
      181 gttcccctcg ccggcggccg cgtcgagcgc ccggcggaaa ttgtccacga tgtcgaggag
      241 gtccgacagg acgcggtcgg cggccgtctt caccagttcg tcgtactggc gcgcctgccg
      301 tttgcggtag ttgtcgaaat cggccgccgt ccgcagcagg cggtcctcca gctcggcgat
      361 ccgtcgccgg agctgctcct cttccgggat ctcccctggc ggcgggcccg gttcggcgtc
      421 gccgttttct acgccggccg ccggaggcgc ctcccctgat ccgctgcgct cgacactttc
      481 gcacggctgc cctcccggcg ccaccgggtc ttccggctcg atatccttct tacgcatcct
      541 tcgattctcc ctctttgctg ctcatgcccg agagcacctc ggtgatcgcg cgcgccgtgt
      601 agtcgacgat cgacaccagc ttcccgtact ccatgcgcgt cggcccgatt atgccgatgg
      661 tcccggcaat actcccgact ttgtatgacg acgtcaccag ggagcagttc agaatctcat
      721 caaacttgtt ctcccgcccg atggtgatga acagcccttc ttcgtaggcg ctgctgagaa
      781 actccttgag cgtacggttg tcctcgagca ggcggacaat gcgcgtgatg cggtcgatct
      841 cggagaattc gggctggctg agcagccgct cgactccggc gaggtggatc gcccccgccg
      901 gatcatccgc ccagatgcgg tcccgcgaat cgatgatgag cttgaccagg cgccccgacc
      961 cggtgtggtc ggccagccgc ttgctgatcg tggtgcgaat ctcccccagc gacagcccgg
     1021 cgagccgctc gttgagcacc tgctccacct cgcggagcga ggcttcgtcg atgctcgttt
     1081 cgacctcgac aatcacgctg cgggccagcc ccgattcgac caccacgacc gccatgatcc
     1141 gaccctgcga cagcgaaatc aactcgatcc ggcgcaacac cccccggtca aagcgcggcg
     1201 cgatcgacac tccgagctga ttggtgatat cgctcagcac ccggcaggtc tgccccagga
     1261 tatcatccaa cccccgtccg tggctcacga tcacatcctt gatccgctcc ttctccgact
     1321 ccgtgagatc ctccggcttc aggaggtagt ccacgtacac ccggtacccc aggtcggtgg
     1381 ggattcgccc ggccgaggtg tgcggctgac gcaccagccc cagctcctca agatcctgaa
     1441 gcgtgttgcg gatcgtcgcc gaagacactc ccatccggaa cttgtgggcg atcacccgcg
     1501 acccgaccgg atctgccgaa cggatgtagt gctcgatcag attcgacaga acctgctttt
     1561 cgcgctctgt taaattctca aatgccataa cagcttagtg tttacccacc gggggctcct
     1621 gccattttgg cagcatacct gtcagaccgc taacaaatgt atacgattta taacgcaagt
     1681 caagcccaaa tgattccggc gatcacaact tctcacgt
//
LOCUS       DAOOKS010000488         1717 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_89842_length_1717_cov_3.102286, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000488 DAOOKS010000000
VERSION     DAOOKS010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89842_length_1717_cov_3.102286"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1063)
                     /locus_tag="PK112_07745"
     CDS             complement(<1..1063)
                     /locus_tag="PK112_07745"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HPC11975.1"
                     /translation="MSAEAAAPESRTIAGTPFFANARPGDGPRCALPTLRPCAHYFLL
                     RKPWSLRSRPTTHHHHATDFRGRPAAIVFAAGEADVVAAVRFCAERGLPLVGRGAGTG
                     LSGGCVPANGELVVSTERLTELSIDPARRIAFCGPGVITKHLQDQALVHGLAYPPDPA
                     SFDQSTLGGNVAENAGGLRCKRFGVTRDYVLGVRGVTGEGRLLETGIFNQNRGFDLMD
                     ILIGSEGTLAIITRIALRLILAGATGITILVAFRQPEDAARAVAEITGSGLIPTVLEY
                     VDGDAAAAANAYRRTEGIDRAAAVLLIETTERDPEEETAHIRAVCERHRASYLRVEPD
                     AQAAETLWDVRRNISTALKA"
     gene            complement(1060..1305)
                     /locus_tag="PK112_07750"
     CDS             complement(1060..1305)
                     /locus_tag="PK112_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HPC11976.1"
                     /translation="MPTYQYVCNECGCRFEEFQRISELPLTECPKCQGYVERVISGGA
                     GFLFKGSGFYITDYRKKDYKDAAQKETSGITTKTENS"
     gene            complement(1414..>1717)
                     /locus_tag="PK112_07755"
     CDS             complement(1414..>1717)
                     /locus_tag="PK112_07755"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPC11977.1"
                     /translation="AALRAIPGITCPNADGAFYLFPDISGLLGGKVGDRPMMTSADLA
                     LFLLERHGVAVVPGSAFWAENHLRISYAAAEHRLVEGLRRLGEGVAQLRPRVRNGN"
BASE COUNT          303 a          526 c          592 g          296 t
ORIGIN      
        1 tcgctttgag ggcggtcgag atgttgcgtc tgacgtccca tagagtttcg gccgcctggg
       61 cgtccggctc gacacgcagg tacgaggcgc ggtgacgttc gcagacagcc cggatgtgcg
      121 cagtttcctc ttccgggtcg cgctcggtgg tctcgatcag caggacagcg gcggcgcgat
      181 cgatcccttc ggtcctccta tatgcgttgg cggcggcggc cgcatctccg tcgacatact
      241 ccagaaccgt ggggataagg ccggagccgg tgatctcagc cacggcccgg gcggcatcct
      301 ctggttggcg gaaggcgacc aggatggtta ttcccgtggc gccggcgagg atgagacgga
      361 gagctatccg ggtgatgatg gccagtgtcc cctcggaacc gatgaggatg tccatgaggt
      421 cgaagccgcg gttctgattg aagataccgg tctcgaggag gcggccctcc ccggtcaccc
      481 cccgcacgcc cagcacgtag tcgcgggtga cgccgaaccg cttgcagcgc aggccgcccg
      541 cgttctcggc aacgttgccg cccagggtcg actggtcgaa tgacgccggg tcgggaggat
      601 aggccagacc gtggacgagg gcctgatcct gaagatgttt ggtgatgacg cccgggccgc
      661 agaacgcaat gcggcgggcg gggtcgatcg acagctcggt caggcgctcg gtggacacga
      721 cgagctcgcc gttggccgga acacaccccc cggagagccc ggtcccagcg ccgcgtccca
      781 cgagggggag gccgcgctcg gcgcaaaacc ggacggccgc aacgacatcc gcctcaccgg
      841 cggcaaagac aatggcggcc ggccggccgc gaaaatcggt cgcgtgatgg tggtgcgtcg
      901 tgggcctcga cctcaaggac caaggcttcc ggagaagaaa gtaatgagcg cagggccgta
      961 aagtcggaag cgcgcatcgg gggccgtctc ctggtcgggc gtttgcgaaa aagggcgtgc
     1021 cggcgattgt gcggctctcc ggcgccgccg cttcggcgct cacgaatttt cggtcttggt
     1081 cgttatgccg gacgtttctt tctgggcggc gtccttgtag tccttcttgc gataatcggt
     1141 aatgtagaat ccgctgccct tgaagaggaa accggccccg ccggaaatca cccgctcgac
     1201 atagccctgg catttcgggc attcggtcag aggcagttcg ctgatgcgct gaaactcctc
     1261 gaaacggcag ccgcattcgt tacacacgta ctggtaggtc ggcatcggtc gtccatcact
     1321 ccttgtcaat cagctcacag attctcgccc ggtggcctgt ccggccgggg ccgagacagg
     1381 cgcaccgaca aatatatcac attttacacg gaattagttc ccatttcgta cccttgggcg
     1441 gagctgggcg acgccctctc ccagccgtcg caaaccctcg acgagtcggt gctcggccgc
     1501 ggcgtaggat atacggagat ggttttccgc ccagaaagcg gaacccggca cgacggccac
     1561 gccgtgccgc tcaagcagaa agagggccag gtcggcggag gtcatcatcg ggcggtcgcc
     1621 gactttgccg ccgaggaggc cggaaatgtc ggggaagagg tagaaagcgc cgtcggcgtt
     1681 cggacaggta atgccgggga tggcgcggag ggcggcc
//
LOCUS       DAOOKS010000489         1716 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_89943_length_1716_cov_2.681517, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000489 DAOOKS010000000
VERSION     DAOOKS010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89943_length_1716_cov_2.681517"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..951)
                     /locus_tag="PK112_07760"
     CDS             complement(<1..951)
                     /locus_tag="PK112_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11978.1"
                     /translation="MSKWVSTLLGLLLVASSAVAGGGEVWIATFDGTMGLQDQARGIA
                     VDPSGCVFVVGETNYASEPVEIPSSLLLIKFSPDGDTLWTHVNDSASSARYLVVDDSG
                     CVYVAGESKSDTVDFLVLKYGPEGDSIWAATYKHPVFTSDEIRGIVLDPNRNLYLLGD
                     VEDLADDEDIALVKILPDGSIDWVSILSGPAGDDQPEGIAVDNSGHIAVVGSSRNSDS
                     ATTAIWTIMYDASGDTVWTARYGGSTTCYALSVGTDDEGSVYVAGELGEYETADCIFI
                     KYSAAGVFQWDWTYSGPAGLRDYIGDLHVGGDGSVWLSIVR"
     gene            1192..>1716
                     /locus_tag="PK112_07765"
     CDS             1192..>1716
                     /locus_tag="PK112_07765"
                     /inference="COORDINATES: protein motif:HMM:NF025912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPC11979.1"
                     /translation="MFDLNPKEAQSSVERPAAGAYWPAPAAAALAAGKYSRAVELCRE
                     GLAEEPGAVAGRLIYARALYHAGQVESAAEQFYEVLARDPENAAALKYLGDIKWAEGD
                     VHGAVAHWRRVLEIDPYCRELRCDWRARERAADEPGSPASGEDQTASPSNAGAGEQLR
                     TAAAGNGPAGEGRAT"
BASE COUNT          340 a          544 c          556 g          276 t
ORIGIN      
        1 acggacgatg ctcaaccaga cggaaccgtc tccgccgacg tgcaggtcac cgatgtaatc
       61 gcgcagaccg gcagggccgc tgtaggtcca gtcccactga aaaacgcccg ccgcgctgta
      121 tttgatgaag atgcagtccg cggtttcgta ctccccgagc tccccggcga cgtacacgct
      181 tccctcgtcg tccgttccca cactgagtgc atagcaggtc gttgagcccc cgtaacgcgc
      241 cgtccaaact gtatcgcccg acgcatcgta catgatcgtc cagatcgccg ttgtcgcgct
      301 atcggaattc ctgctcgagc cgacgacagc gatgtgacca gaattgtcga cagctattcc
      361 ctcgggttga tcgtcgccgg caggaccgga gaggatggaa acccagtcga tggaaccatc
      421 cggcagaatt ttcacaaggg caatgtcctc atcgtctgcc aaatcctcga catcgcccaa
      481 caggtacagg ttgcggttcg ggtcgagcac aatgccgcgg atctcatcgc tcgtaaagac
      541 agggtgcttg taggtcgccg cccaaatcga gtcgccctcg gggccgtact tgaggaccag
      601 gaaatcgacg gtatccgact ttgactcgcc ggctacatag acacagccac tgtcgtcgac
      661 gacgagatac cgggcactgg atgcgctgtc gttcacgtgc gtccacaggg tgtcgccatc
      721 cggcgagaac ttgatgagca gcaggcttga tgggatttcg accggttccg aggcgtaatt
      781 ggtttctccg acgacaaaca cgcatccgga aggatccacc gcgatgccgc gggcctgatc
      841 ttggagcccc atagtcccgt cgaacgtcgc aatccacacc tcgcctccgc cggcgaccgc
      901 cgaggacgca accagcagca aacccaagag agtggaaacc cacttactca tgaaaaaacc
      961 tcctccttgt tgtatcgagg gtggccaagc atcagtctgt ggcctaaccc tgcgctggaa
     1021 ttcacgcccg cgtgtgggca aaccgctcca cattggcaag tacggtcacc gcggcagcaa
     1081 agtcaagcgg aagcgccgga tccggccggg gagccgccga agagagagaa cgggcgactt
     1141 cgccccgccc ggcgcttgcg cggccgcgcc ttttgcggta ccttcacttt tatgtttgac
     1201 ctgaacccga aggaagcgca gtcgtcggtc gagcggccgg cggcgggtgc ctactggccg
     1261 gcgccggcgg cggccgcgct cgccgcggga aaatactcgc gggcggtcga actctgccgc
     1321 gagggactgg ccgaagagcc gggagcggtc gcggggcggc tgatttacgc gcgggcgttg
     1381 taccacgccg ggcaggtgga atcggccgcc gagcagttct atgaggtgct ggcgcgagat
     1441 cccgaaaacg ccgcggcgct gaaatatctc ggcgacatca agtgggcgga aggggacgtg
     1501 cacggcgcgg tggcccactg gcggcgagtg ctggaaatcg acccctactg ccgcgagctg
     1561 cggtgcgact ggcgcgcgag agagagggcg gcggatgaac ccgggtcgcc cgcctccgga
     1621 gaggaccaga ccgcgtcacc gtcgaacgcc ggggcagggg aacagctcag gacggccgcg
     1681 gcggggaacg gaccggccgg cgagggacgc gcgacg
//
LOCUS       DAOOKS010000490         1698 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_91316_length_1698_cov_4.410834, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000490 DAOOKS010000000
VERSION     DAOOKS010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91316_length_1698_cov_4.410834"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="PK112_07770"
     CDS             complement(<1..423)
                     /locus_tag="PK112_07770"
                     /inference="COORDINATES: protein motif:HMM:NF014769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide diphosphorylase
                     (carboxylating)"
                     /protein_id="HPC11980.1"
                     /translation="MRDQSLWLVELVKAALGEDVGPGDLTSMGCLEPNPAKARIVAKQ
                     DGVLSGLRPMLLVFGIVDSANTVRVFKNDGDRFAKGETLVEIEGFNQSMLAGERVALN
                     FLSHLSGIATLTAKFVAAVAGTGARILDTRKTTPGMRVL"
     gene            487..1434
                     /gene="ilvC"
                     /locus_tag="PK112_07775"
     CDS             487..1434
                     /gene="ilvC"
                     /locus_tag="PK112_07775"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HPC11981.1"
                     /translation="MKDLSTGGPVAVIGYGSQGHAVAQNLRDSGFDVIVGLRPDSPRR
                     ADARRDGMPRIDGIGAAVGQAGGVVFAFPDHRHGVVYRREIRDRLRPGAALVFLHGLS
                     VHFGLVEPPEDADVILIAPHGPGVAVRANYLAGDRSMSAFVSVHRDSSGQAGPTMFGL
                     AAGMGFDLTRLVPTTFEAEAVGDMFGEQAVLCGGLAALITGGFEVLVKNGIPPDAAYL
                     EVAYQLDLIVALIKKHGIRGMFERISLTARYGSLETGPFLIDEVVKARMQQVYDRIAS
                     GEFVSRLTRLSEAQIDELGADLARLTDEKLEQAARKFKG"
     gene            complement(1455..>1698)
                     /locus_tag="PK112_07780"
     CDS             complement(1455..>1698)
                     /locus_tag="PK112_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HPC11982.1"
                     /translation="RVEAGRIADIRSQTFGCAAAIACSSMASEMVLGKTLDEAFALTR
                     DDVAQALGGLPEKKMACSNLAPDAIRAAIEDYRARR"
BASE COUNT          255 a          557 c          599 g          287 t
ORIGIN      
        1 caggacccgc atccccggcg tggtcttgcg cgtgtccaga atacgggcgc ccgtacccgc
       61 gacggcggcg acgaatttgg cggtcagggt cgcgatcccg ctcaggtgcg acagaaagtt
      121 gagggccacc cgttcgccgg cgagcatgga ttggttgaag ccctcgattt cgaccagcgt
      181 ctcccccttg gcaaaccggt cgccgtcgtt tttgaacacg cggaccgtgt tggcggagtc
      241 gacgatgccg aagaccagga gcatggggcg gagccccgac aggacgccgt cctgcttggc
      301 gacgatcctg gccttggcag gattgggctc gaggcacccc atggaggtga ggtcccccgg
      361 cccgacatcc tcgcccaggg ccgctttcac cagttcgacg agccagagcg attgatcgcg
      421 catactggga aaacaatatg gcggcgggca attgtcaacg atcaaacggc gggctatatt
      481 ggcgccatga aggatctttc gaccggcggc ccggtggccg tcatcggcta tggatctcag
      541 ggtcacgcgg tggcccagaa ccttcgcgac tccggctttg acgtcattgt gggtctccgc
      601 cccgactccc cccggcgcgc ggatgcacgc cgcgacggga tgccccggat cgacgggatc
      661 ggggcggcgg tcgggcaagc ggggggcgtg gttttcgcct ttcccgacca ccgccacggc
      721 gtcgtgtatc gccgcgagat ccgcgaccgc ctgcgccccg gcgcggccct ggtctttctc
      781 cacggcctct cggtgcattt cggtctggtg gaaccgccgg aggacgccga cgtgatcctg
      841 atcgccccgc acgggccggg agtggcggtg cgggcgaact acctcgccgg cgaccgcagc
      901 atgtcggctt tcgtctcggt ccaccgggac agttccggcc aggccggccc cactatgttc
      961 gggttggcgg cggggatggg gtttgacctg acccgcctgg taccgacgac gttcgaggcc
     1021 gaggcggtcg gcgacatgtt cggcgagcag gcggtcctgt gcggggggct ggccgcgctg
     1081 atcaccggcg gattcgaggt gctggtgaaa aacggcattc cgcccgacgc cgcctacctc
     1141 gaagtggcct accagctcga tctgattgtc gcgctcatca agaagcacgg gatcaggggg
     1201 atgttcgagc gcatctcgct gaccgcccgc tacggctcgc tcgagaccgg tccgtttctg
     1261 atcgacgagg tggtgaaggc ccgcatgcag caggtgtacg accgtattgc ctccggcgaa
     1321 ttcgtctcgc ggctcacccg gctcagcgag gcgcagatcg acgagctcgg cgccgacctg
     1381 gcccggctca cggatgagaa gctggagcag gcggcgcgga agttcaaggg gtagccgggg
     1441 gcggggcggc gcgctcaccg ccgcgcgcgg tagtcctcaa tggccgcccg gatcgcatcg
     1501 ggggcgaggt tgctgcacgc cattttcttt tccggcagcc cgccgagcgc ctgggcgacg
     1561 tcgtcgcgcg tcagcgcgaa ggcctcgtcg agcgttttcc caagcaccat ctcggacgcc
     1621 atcgagctgc aggcgatcgc cgcggcgcag ccgaacgtct gggagcggat gtcggcgatg
     1681 cggccggcct cgactctg
//
LOCUS       DAOOKS010000491         1697 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_91426_length_1697_cov_2.216200, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000491 DAOOKS010000000
VERSION     DAOOKS010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91426_length_1697_cov_2.216200"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..594
                     /locus_tag="PK112_07785"
     CDS             76..594
                     /locus_tag="PK112_07785"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HPC11983.1"
                     /translation="MALRQLIFIVTLSAVLGLAANLVSPHAIPYIGTWRELSSGDGPI
                     KPPEHAPGDPPYIGIDVAHVEYTGGGVIFIDARSPEEFACGTIPGAVNIPFDELPEGD
                     LTPYFDSALAGAPRDTRLITFCSGEECDLSLHLARNLQAVGYTDLLIFFGGSREWEKN
                     GLEMERRAKCDG"
     gene            582..1064
                     /locus_tag="PK112_07790"
     CDS             582..1064
                     /locus_tag="PK112_07790"
                     /inference="COORDINATES: protein motif:HMM:NF018942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HPC11984.1"
                     /translation="MRRLIDNDYLTLLIRLFVGGVFIYASYYKIINPMDFAKSIWYYH
                     MVPGSLINLMAIVLPWWELVAGVCLVLGVWYRGTVWSMLLMTVMFGAALASAAIRGLD
                     IQCGCFKASVGAGDEAAATLVRDLGLFVLTLQLLFSRARRFFVGRARPLTEGRAALSR
                     "
     gene            complement(1033..>1697)
                     /locus_tag="PK112_07795"
     CDS             complement(1033..>1697)
                     /locus_tag="PK112_07795"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II and III secretion system protein"
                     /protein_id="HPC11985.1"
                     /translation="FTWPTVLTATLGGDSASTGGSGLSGSSLAWDPNNGDLSWARLSA
                     EQVSVMLHLLEQGGNSRLISDPHVTTLAIHEAEIKIQTVIPIATINRFTEGAVIQDIV
                     TFQDEEVGISLRVTPRVNREGMITMEVIPVVEDIIDFTGPVDNQKPITTERSVRTTVA
                     VAEGETLALGGLRKESELKQKQRVPLLGRIPLLGNLLFTYTSNEKKTTDLIILITPRV
                     LP"
BASE COUNT          282 a          501 c          570 g          344 t
ORIGIN      
        1 ccgaaaggcc aaaccttcgg accgctggtc tgtataaccg accaaaggaa tccgactcgc
       61 aagcgagcag acagcatggc gttacgacaa cttatcttca tcgtcaccct ctcggcggtt
      121 cttggtctgg cggccaatct ggtctcgccc cacgctatcc cgtacatcgg gacatggcgg
      181 gagttgtcat cgggcgacgg gccgatcaag ccgccggagc atgcgccggg cgatcccccg
      241 tatatcggga tcgatgtcgc ccatgtcgag tataccggcg gcggcgtgat tttcatcgat
      301 gcgcggtcgc cggaggaatt cgcctgcggg acaatcccgg gcgcggtcaa tatcccgttt
      361 gacgaactcc ccgaggggga tctcaccccg tatttcgatt ccgccctggc cggagcgccc
      421 agggacaccc ggctgatcac gttctgctcg ggggaggagt gcgacctctc gctgcacctg
      481 gcccgcaacc tgcaggcggt cggttacacg gacctgctga tttttttcgg cggctcgcgg
      541 gagtgggaga agaacgggct ggaaatggaa cggagggcga aatgcgacgg ttgatcgaca
      601 acgactatct gacgctgctc atccggctgt tcgtgggggg tgtgttcatc tatgcgtcct
      661 actacaagat catcaatccg atggatttcg ccaagagcat ctggtactac cacatggtgc
      721 cggggagtct gatcaacctg atggcgatcg tgctgccctg gtgggaactg gtcgcggggg
      781 tgtgcctggt gctcggagtc tggtatcggg ggacggtgtg gtcgatgctc ctgatgacgg
      841 ttatgttcgg ggcggcgctg gcctcggcgg cgattcgggg gctggacatc cagtgcggct
      901 gtttcaaggc ctcggtgggg gccggcgacg aggccgcggc cacgctcgtg cgcgacctcg
      961 gcctcttcgt gctcaccctt cagttgctct tctcgcgcgc gcggcggttc tttgtcggcc
     1021 gcgcacggcc acttacggaa ggacgcgcgg cgttatcaag atgatgaggt cggtcgtctt
     1081 cttttcgttc gacgtgtagg taaagaggag atttcccagc agcgggatgc ggccgagcag
     1141 cggcacccgc tgtttctgct tcagctcgct ctctttgcgc agcccgccca gggcaagcgt
     1201 ttctccctcg gcgacagcca ccgtcgtccg caccgagcgc tccgtggtga tcggtttctg
     1261 gttgtccacc gggcccgtga aatcgatgat atcctcaacg accgggatca cttccatcgt
     1321 gatcatcccc tcgcggttga cgcgcggggt gacgcgcagg gagatgccga cctcctcgtc
     1381 ctggaaagtg acgatatcct gaatcaccgc cccttcggtg aagcggttga tagtggcgat
     1441 gggaataacg gtttggatct tgatctcggc ctcgtgaatg gccagggtgg tgacgtgcgg
     1501 atcggaaatg agacggctgt tgccgccctg ctcgagcagg tggagcatga cgctgacctg
     1561 ctcggcggag agccgcgccc aggagaggtc gccgttgttg ggatcccagg cgagcgagga
     1621 acccgacaga ccggacccgc cggtcgaggc cgaatcgccg ccgagggtgg cggtcagcac
     1681 ggtcggccag gtgaagc
//
LOCUS       DAOOKS010000492         1691 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_91862_length_1691_cov_3.446210, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000492 DAOOKS010000000
VERSION     DAOOKS010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91862_length_1691_cov_3.446210"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="PK112_07800"
     CDS             complement(<1..430)
                     /locus_tag="PK112_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="With MotB forms the ion channels that couple
                     flagellar rotation to proton/sodium motive force across
                     the membrane and forms the stator elements of the rotary
                     flagellar machine; Derived by automated computational
                     analysis using gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HPC11986.1"
                     /translation="MFIFIGALIVLGGVLGGYTIHGGKILALNQPSELLIIGGAALGS
                     LLISTPLPVVRGIFAQLKQALGGGLKKKDYLSLLVMMFEIFNVARKDGLIGLESHVEH
                     PEESEIFKRYPGFLKNHHAVAFFTDTMRVIISGAVQPHDLE"
     gene            661..1446
                     /locus_tag="PK112_07805"
     CDS             661..1446
                     /locus_tag="PK112_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010521115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HPC11987.1"
                     /translation="MRFFGAVLPPRRGLAAGRLAGFVGLAALLAGLLLPRASLSQPTI
                     DLSRQRMPQAMPGRGANPNPSALKVARLHYSGGGDWYWGSSALPNFLTYLNRQTDFPV
                     DTMERVVTIMDADLFRYPFLFATGHGVIRFEAEERERLRRYLEGGGFLFVNDSYGMEE
                     TFKKEMAELFPEREPVAVPFDHPIYRCFYDFPNGPPKIHEHDGQPPRAWAIILHGRIV
                     LYFLVESDIGDGWEDPQVHNDPPELREQAFRMGVNLVTYALLY"
BASE COUNT          275 a          510 c          585 g          321 t
ORIGIN      
        1 cttcgagatc gtgcggttgg acggctccgg agatgatcac gcgcatggtg tcggtgaaga
       61 acgcgaccgc atggtggttc ttcagaaacc cgggatagcg cttgaatatc tcgctctcct
      121 ccggatgctc gacgtggctc tccaggccga tgaggccgtc tttgcgcgcc acgttgaaga
      181 tctcgaacat catgaccagc aggctgaggt aatctttctt cttgaggccg cccccgagcg
      241 cctgcttgag ctgcgcgaag atgcccctga ccacgggaag cggggtggaa atcagcagcg
      301 agccgagggc ggcgccgccg atgatgagga gttcggaggg ctggttgagg gcgaggattt
      361 tgcccccgtg gatggtgtaa ccgccgagga caccgccgag gacgatcagg gcaccaatga
      421 atatgaacat agcgcgttgt ccctgtgtaa gttatgggtc attggccgac tgccggttct
      481 tgtacattat cgtcacgtcg gcggcaaagc tgaagggcgg cgcggagcac gggcggggcg
      541 ggggcgatcg agcagcggcc ggccgggggc tggaccgtcc gccagcccgc agcgcgccgt
      601 tgggccggac gcggcaactt tcctgcatca aaaatgggac tctgcctgta tattatgtgt
      661 atgcggtttt ttggcgcagt actcccccct cgacgggggt tggcggccgg ccggctggcc
      721 gggtttgtgg gcctcgcggc gctgctcgcg ggactgcttc tcccccgcgc ctccctgtct
      781 cagccgacga tcgacctctc ccggcagcgg atgccgcagg cgatgcctgg ccggggcgcc
      841 aacccgaacc cttcggcgct gaaggtcgcc cgcctccact actccggcgg cggcgactgg
      901 tactggggga gctcagcgct ccccaatttc ctaacatacc tgaaccggca gaccgatttc
      961 ccggtcgaca cgatggagcg ggtggtgacg atcatggatg ccgacctgtt ccgctacccc
     1021 ttcctgttcg cgaccggcca cggggtgatc cggtttgagg ccgaggagcg ggaacgtctg
     1081 cgccggtatc tcgagggggg cgggtttctg ttcgtgaacg attcctacgg catggaggag
     1141 acgttcaaga aggagatggc ggagctgttc ccggagcggg aaccggtggc ggtgccgttc
     1201 gaccatccga tttaccgctg tttctacgat tttcccaacg gtccgccgaa gatccacgag
     1261 cacgacggcc agcccccgcg ggcgtgggcg atcattctcc acggccggat cgtgctgtat
     1321 ttcctggtgg aatcggacat cggggatggc tgggaggatc cgcaggtgca caatgacccg
     1381 ccggaactgc gggaacaggc gttccggatg ggcgtgaacc tggtgaccta cgccttgttg
     1441 tactagtcgg gctgcgcgag attacctgcc gggccggcgg cctacctgtc gatagaaaga
     1501 atgcgcggct tcgccggcga agccgccgcc tgagaccgaa caccgagggt atcgctatga
     1561 ccgagttgac ctcccgccgc gcgctccttg ccaagctcac cggggtgctg gtgcgccagc
     1621 gcgcagtgct gtttgtatcc ggcgtcatgt tgacggccgc cgcggtgacg gcggcggcga
     1681 tcgggctgtc g
//
LOCUS       DAOOKS010000493         1688 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_92110_length_1688_cov_2.552358, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000493 DAOOKS010000000
VERSION     DAOOKS010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92110_length_1688_cov_2.552358"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..819
                     /locus_tag="PK112_07810"
     CDS             148..819
                     /locus_tag="PK112_07810"
                     /inference="COORDINATES: protein motif:HMM:NF020771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CerR family C-terminal domain-containing
                     protein"
                     /protein_id="HPC11988.1"
                     /translation="MAAPYQDMRTDSTARGVQERLIGAGEDLFSRRGFNETSVRDIAA
                     AAGCNVASINYYFGGKENLYVEIWRRRLSQIRDARVASIEKVMSSTGRPKLEDLLRSH
                     AVSFLEPLLKGGQQCPLVNLIAREMVDPHLPQGMFLEEMAMPVMSVLGGALMAICPWL
                     DKTKIQALVLFLVGQLVHAVCAREMFEKSGRPGVIHMSIEGLIEDIVKFSAGGIRAYA
                     NDNGA"
     gene            860..>1688
                     /locus_tag="PK112_07815"
     CDS             860..>1688
                     /locus_tag="PK112_07815"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPC11989.1"
                     /translation="MIAFESTKGMKAVRTGVYLSVAAVVVSALLAGCGSERQMAREVR
                     QARVEAYRQWESRKMAEDEQQPVISGRLNLADSVKLTLAHNKMLRKAMEEREAARGAG
                     VGARSAYLPSLSFSGEYQRLDEVSSFEMETPDGRQSFQLGDVDNYSVGLTVSQPIYAG
                     GAISARVRVARLMSLSADQTIRASMQDVVYAAETAYYGLLLSQHLVEISMDAVRSSKA
                     HLENVEKRRASGVASDFDVLRAEVELSNFEADLIRSKNAINISRANLIKYMGVSQNSD
                     "
BASE COUNT          344 a          438 c          564 g          342 t
ORIGIN      
        1 ccccaaactc aaggctgtaa ctctttctca taacataggt tgagtgtgtt tgtctattcc
       61 tgtggcggga acgggaagaa aaactgttga tatcttaaac attcgtttaa aatggccgta
      121 tgaaacggtt gtttggagat aggcatcatg gcagcaccgt atcaggacat gcgcacggac
      181 agcaccgccc ggggtgttca agaaaggttg attggcgcgg gggaggacct gttcagccga
      241 cgggggttca acgagacgag tgttcgcgac atcgccgctg cggccggctg caacgtggcg
      301 tccatcaact actacttcgg cggcaaggag aacctgtacg tggaaatctg gcgccgccgg
      361 ctgtcgcaga taagggacgc ccgggtggcc agtatcgaga aggtcatgtc gagcacaggc
      421 cggccgaaac ttgaagacct gctcaggtcc cacgcggttt cgtttctgga gcctctgctc
      481 aaggggggcc agcagtgtcc tctcgtcaac ctgatagccc gggagatggt cgatccgcat
      541 ctgccgcagg ggatgttcct ggaggagatg gcgatgccgg tgatgtcggt cttgggtggg
      601 gctctgatgg cgatttgtcc gtggcttgac aagaccaaga ttcaggcgtt ggtccttttt
      661 ctggtcggcc agttggtaca tgcggtgtgt gccagggaga tgttcgagaa aagtggccgg
      721 ccgggggtga tccacatgag catcgaagga ctcattgagg atatcgtcaa gttctcggca
      781 ggcggcattc gcgcgtacgc aaacgacaac ggcgcatagt ttccggcgat gaatgaaatg
      841 ttcgaagggc gggagaacga tgattgcttt cgaatcgaca aaagggatga aggctgtgag
      901 aacgggtgtt tacctgtctg tggctgcggt tgttgtctcc gcgttgctcg cgggctgcgg
      961 cagcgagcgg caaatggctc gcgaggttcg ccaggcgcga gtggaagcgt accggcagtg
     1021 ggagagccgc aagatggcgg aggacgagca gcagcctgtc atcagcggca ggctcaactt
     1081 ggccgacagc gtcaagctga cgctggccca caacaagatg ctgcgcaagg cgatggagga
     1141 acgcgaggcg gctcgtggcg ccggggttgg ggctcgctcg gcatatctgc cgagcctctc
     1201 gttctccggc gagtatcagc ggctcgacga ggtctcttcc tttgagatgg agactcccga
     1261 tggaaggcag agcttccagc tcggcgacgt ggacaactac tcggtgggcc tgaccgtcag
     1321 ccaaccgatc tacgcaggcg gcgccatctc cgcgagggtg cgggtcgcga ggttgatgtc
     1381 gctgtccgcc gatcagacga ttcgggcctc catgcaggac gtagtctacg cggccgaaac
     1441 cgcctactac ggtctgttgc tcagtcaaca cctggtcgag atcagcatgg atgccgtgcg
     1501 gtcgtcgaag gcgcatctgg agaatgtcga gaagcggcgg gccagcggcg tcgcctccga
     1561 tttcgatgtc ctgcgggccg aggtcgaact ctcgaatttc gaggccgatc tgatccgcag
     1621 caagaacgcc atcaacattt cccgagccaa tctgatcaag tacatgggtg tctcgcagaa
     1681 cagcgatt
//
LOCUS       DAOOKS010000494         1684 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_92459_length_1684_cov_2.190301, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000494 DAOOKS010000000
VERSION     DAOOKS010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92459_length_1684_cov_2.190301"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="PK112_07820"
     CDS             complement(<1..207)
                     /locus_tag="PK112_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HPC11990.1"
                     /translation="MAAMTCAEVRDMLHESLGADELSGEAAAHLAGCPACRALHEELR
                     ATAEGARTAGEFELTDLESARLLAR"
     gene            complement(195..770)
                     /locus_tag="PK112_07825"
     CDS             complement(195..770)
                     /locus_tag="PK112_07825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPC11991.1"
                     /translation="MAGNDRETIDRLVRQAQNGDRAAFSQLVRRTMRDITALTYRMTG
                     DRDAAQDLAQETFVSAWQHLSGFRREARFENWLYRIAANKALNYLGRGGAGRTVSLED
                     AGGAGAERASDEPAPDRALEQARLRDDISAFMRLLPPRQRLVFELRFYRQLGFAEIAE
                     MTGTAVGTVKTNYREAVGKLRTYAAERGWRP"
     gene            complement(890..>1684)
                     /locus_tag="PK112_07830"
     CDS             complement(890..>1684)
                     /locus_tag="PK112_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HPC11992.1"
                     /translation="QISEKLISDFIRRVVKHKYLMKPRVVICVPSGITEVEKRAVRDS
                     AENAGAREVYLLQEPMAAAIGVGLPVEQPTGSMVIDIGGGTSEIAVIALSGIVNNTSI
                     RVAGDEMNEAITQYLKKNYNLAIGELTAEDIKIKIGSAFALDKEISMEIKGRDLVAGV
                     PKNLNLSSVQVREALSETIDIIVEAVRQALEQTPPELASDILERGIILTGGGALLRGL
                     DKRLRQETNLPVNVADDPLTCVVRGTGKVLENFTAFQKVLERPRKD"
BASE COUNT          250 a          571 c          558 g          305 t
ORIGIN      
        1 ccgcgccaga aggcgggcgc tctcgaggtc ggtgagttcg aactcgcccg ctgtccgcgc
       61 cccctcggcc gtcgcccgca attcctcgtg cagagcgcgg caggccggac acccggcgag
      121 gtgcgccgcc gcctcgcccg aaagctcatc ggcccccagc gattcgtgga gcatgtcccg
      181 cacttcggcg caggtcatgg ccgccacccc ctctccgccg catacgtccg cagtttgccc
      241 accgcctcgc ggtagttggt cttcaccgtt ccgaccgcgg ttccggtcat ctcggcaatc
      301 tcggcgaacc cgagctggcg gtagaagcgc agctcgaaca ccaggcgctg gcggggaggg
      361 aggagccgca tgaaggcgct gatgtcgtcg cggaggcggg cctgctccag cgcccggtcg
      421 ggcgcgggtt catcggaggc gcgctcggcc cccgccccgc cggcgtcctc gagcgacacc
      481 gtgcggccgg cccccccgcg gcccaggtag ttcagcgcct tgttggccgc aatccggtac
      541 agccagttct cgaagcgggc ctctcggcgg aacccggaga ggtgctgcca ggcggagacg
      601 aaggtttcct gcgccaggtc ctgggcggcg tcccggtcgc cggtcatccg gtaggtcagc
      661 gcggtaatgt ctctcatagt ccgccgtacc agttgcgaga acgccgctcg gtcgccgttt
      721 tgcgcctgcc tgaccaggcg gtcgattgtc tcacggtcgt tcccagccat gcatttaatt
      781 tccgccgata agaccggccc ggcaatccaa aagattaatg gccgcggagg agtgtatgca
      841 aaaaggccgg acggcagtcc ggcccggcca ggtccgaggg cggcgcgggt cagtctttcc
      901 gcggccgctc cagcactttc tggaaggcgg tgaagttctc cagcaccttg ccggttcccc
      961 gcaccacgca ggtgagcgga tcatccgcca cgttgaccgg gagattggtc tcctggcgca
     1021 ggcgcttgtc caacccgcgc aggagcgccc cgccgccggt caggatgatc ccgcgctcga
     1081 ggatgtccga ggccagctcc ggcggcgtct gttcgagcgc ctgccgcacc gcctcgacga
     1141 tgatgtcgat cgtctcggag agggcttccc gcacctgcac cgaggacagg ttgaggttct
     1201 tggggacgcc ggccacgagg tcgcgcccct tgatctccat cgagatctcc ttgtcgaggg
     1261 cgaaggccga cccgatcttg atcttgatat cctcggcggt gagttcgccg atcgccaggt
     1321 tgtagttctt cttgaggtac tgggtgatcg cctcgttcat ttcgtcgccg gcgacgcgga
     1381 tcgaggtgtt gttgacaatt ccgctgaggg cgatgacggc gatttccgag gtcccgccac
     1441 cgatgtcaat gaccatggag ccggtgggtt gttcgacggg gaggccgacg ccgatcgcgg
     1501 ccgccatcgg ctcctgcagc aggtagacct cgcgggcgcc ggcgttctcg gccgaatccc
     1561 gcaccgcccg cttttctacc tcggtgattc ccgagggcac gcagatgacg acgcgcggct
     1621 tcatcaggta cttgtgcttg accacgcggc ggatgaaatc ggaaatgagt ttctcggaaa
     1681 tctg
//
LOCUS       DAOOKS010000495         1681 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_92695_length_1681_cov_3.603936, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000495 DAOOKS010000000
VERSION     DAOOKS010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92695_length_1681_cov_3.603936"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            403..>1681
                     /gene="ligA"
                     /locus_tag="PK112_07835"
     CDS             403..>1681
                     /gene="ligA"
                     /locus_tag="PK112_07835"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HPC11993.1"
                     /translation="MTTPPRKIIDELEKLRREIRRHDVLYYVEDRPEITDAEYDRLFD
                     RLLAIERDYPDLVAPDSPSQRVGAAPSKRFEPVRHRLPMLSLQKVTDRAGFEDFDRRV
                     RQILETGEVAYLVEPKLDGLAVELVYRDGLFVEGSTRGDGETGENITPNLRTIRNLPL
                     RLSHGAARKYPLLEVRGEVILKRSAFERLNRRLREAGQPMLANPRNGAAGSLRQLDPK
                     ITAARPLVFFAYGVSERTLPGLPGQSATAAFLRREGFSVPDFMELVSGVDAVDRAFAR
                     LAASRPELDYEIDGMVVKVDSYAHQEALGQISRAPRWAVAWKFSAEQAETILEGVEFS
                     VGRTGTVTPVARLRPVAVGGVTVSSATLHNEDEIARLDVRVGDTVIVQRAGDVIPAVV
                     SVVADKRPAHTRPIVFPKACPSCTEPLVRPEGEA"
BASE COUNT          264 a          654 c          474 g          289 t
ORIGIN      
        1 ccggccagga gatcggcgca ttccgcaaaa aacaccgctc ccaccgctct ttcttcgcgg
       61 gctaggcgtc gtcttcaccc cttggcgcac gaccggtccg gtccccccgg attccatgta
      121 cgtcgcgacc ctcgcggtcc ggacacccct gccatcgacg aagaccatcc tcctgatctc
      181 ttgcacgtca ggacccttgt ggtcctgtca tttttgccat cgctgcgagc ggtcccgtca
      241 ggaccctgaa ggtcctgacc tacaaaagcc ccgtcgcgcg ggttctggcg aaactctgtc
      301 ctgtcctaag actttgtgat cgccgtgcgt ggaccttgaa tgtcagcccc ggccccccaa
      361 aaatgattga cacctccccg accgttgtct caaattacgc gcatgacgac cccgccccgc
      421 aagatcatcg atgaactcga aaaactccgc cgcgaaatcc gccgccacga cgtcctctac
      481 tacgtcgagg accgcccgga aatcaccgat gccgagtacg accgcctctt cgaccgcctc
      541 ctcgctatcg agcgcgacta ccccgatctg gtcgccccgg actctccatc gcagcgtgtc
      601 ggggccgctc cctcgaaacg tttcgaaccg gtccgccacc gcctgcccat gctctcgctg
      661 cagaaagtga ccgaccgggc cgggttcgag gatttcgatc gccgtgtccg ccagattctc
      721 gaaaccggcg aggtcgccta tctcgtcgag cccaagctcg acggcctcgc ggtggagctg
      781 gtttaccgcg acggcctctt cgtcgagggt tcgacgcgcg gcgacggcga gacgggcgag
      841 aacatcaccc ccaacctccg caccatccgc aacctccccc ttcgcctctc ccacggcgcc
      901 gcccggaagt acccgctgct ggaggtgcga ggcgaagtga tcctgaagcg ttccgctttc
      961 gagcgcctca accgtcgcct ccgcgaggcg ggccagccga tgcttgccaa cccgcgcaac
     1021 ggcgccgccg gctcgctccg ccaacttgac ccgaaaatca cagccgcccg tcctctcgtc
     1081 ttcttcgcct acggcgtctc cgagcgcact ctccccggcc tgcccggtca gagcgccacc
     1141 gcggcgttcc tgcgccgcga gggcttctcc gtgccggatt tcatggagct cgtctccggc
     1201 gtcgacgccg tcgaccgggc cttcgcaagg ctggccgcct cgcgtcccga gctcgattac
     1261 gaaatcgacg gcatggtggt gaaagtcgac agttacgccc accaggaggc gctcggccag
     1321 atctcccgcg ccccgcgctg ggcggtcgcc tggaaattct ccgccgagca ggccgagacg
     1381 atcctcgagg gcgtcgagtt ctcggtcggc cgcaccggca ccgtcacccc ggtggcccgc
     1441 ctccgcccgg tggcggtcgg cggcgtcacc gtctccagcg ccaccctcca caatgaggat
     1501 gagatcgccc gcctcgacgt tcgcgtcggc gacaccgtga tcgtccagcg cgccggcgac
     1561 gtcatccccg cggtcgtgag cgtggtcgcc gacaagcgcc ccgcgcacac ccggccgatt
     1621 gtctttccca aagcttgtcc ctcctgcacc gagcccctcg tccggccgga gggagaggcc
     1681 g
//
LOCUS       DAOOKS010000496         1678 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_92975_length_1678_cov_3.030807, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000496 DAOOKS010000000
VERSION     DAOOKS010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92975_length_1678_cov_3.030807"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..965)
                     /locus_tag="PK112_07840"
     CDS             complement(<1..965)
                     /locus_tag="PK112_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013150056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HPC11994.1"
                     /translation="MNKQNETEIKKSAAYAPAEVEDRIYRKWLEAGVFVGDPHSPKKP
                     YSIVIPPPNVTDVLHLGHALNNTIQDFLIRRHRMMGYEAEWIPGSDHAGIATQVIVEK
                     RVAKEGTTRREMGREKFVERTWEWANQNKDKILSQLKRMGCSCDWSRTRFTLDPGLSR
                     AVAEVFRRLHEKGWIYRGFRIVNWCPSCQTSLSDDEVEHAEFDSHLWYIKYKLKGSDE
                     YLTVATTRPETMLGDTALAVNPKDTRYKKYVGKTIILPILERDIPVVADSYVDPEFGT
                     GVVKVTPAHDPNDFEIGKRHDLPEINILNADGSLNENAGKFKNLDR"
     gene            1123..>1678
                     /gene="mazG"
                     /locus_tag="PK112_07845"
     CDS             1123..>1678
                     /gene="mazG"
                     /locus_tag="PK112_07845"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HPC11995.1"
                     /translation="MDTIKDRVLDPRRPPFERLVALLAILRSPEGCAWDRKQTHRSLL
                     PYLIEETYEVVEAVENDNPAALREELGDLLVQIVFHAQLAAEAGRFTIDNSIADVVEK
                     LVARHPHVFGERKNLNPRQVRDQWEQIKVESGEKDRVLSGVPKAMPALTMAYRVGEKA
                     GGVGFDWNSPEEVLGKIEEEIGEIR"
BASE COUNT          297 a          559 c          489 g          333 t
ORIGIN      
        1 cggtccaggt tcttgaactt gccggcgttc tcattgagcg acccgtcggc gttcaggatg
       61 ttgatctcgg ggagatcgtg ccgcttgccg atctcgaaat cgttcgggtc gtgcgccggc
      121 gtcactttca ccactccggt gccgaactcg gggtcgacat aactgtcggc caccaccggg
      181 atgtcccgct cgagaatcgg caggatgatc gtcttgccga cgtacttctt atagcgcgtg
      241 tccttcgggt tgaccgccag ggcggtgtcc ccgagcatcg tctccgggcg cgtcgtggcc
      301 accgtgaggt actcgtccga ccctttcagc ttgtacttga tgtaccacag atggctgtca
      361 aactcggcgt gttcgacctc gtcgtccgac agcgacgtct ggcaggaggg gcaccagttg
      421 acgatgcgga acccccggta gatccagcct ttctcgtgca ggcgtcggaa cacctcggcc
      481 accgcccgcg acagccccgg atcgagcgtg aaccgcgtcc gcgaccagtc gcaggaacag
      541 cccatgcgct tgagctggct gagaatcttg tccttgttct ggttggccca ctcccaggtc
      601 cgctcgacaa acttctcccg gcccatctcc cggcgcgttg tcccctcctt ggccacccgc
      661 ttctccacga tcacctgggt ggcgatcccc gcatgatccg acccgggaat ccactccgcc
      721 tcgtacccca tcatgcggtg acgccggatc aggaagtcct ggatcgtgtt gttcagcgcg
      781 tgcccgaggt ggaggacgtc ggtcacattg ggcgggggaa tcacgatgga gtacggcttc
      841 ttgggtgaat gcggatcgcc gacaaacacc cccgcctcca gccacttccg gtatatgcgg
      901 tcctcaacct ccgccggtgc gtaggccgcg gacttcttga tttcggtctc gttttgcttg
      961 ttcatgccgc tttcattcca tatattcgcc gaccggatcg aaccttccgg cgggcggaac
     1021 gtaaaccgcc tagtatactg gcgcccgggg cgtttgtcaa ggcgcgaaag gcgtccgccc
     1081 gcgtgccgct aagtgcgcta attacttata ctatcgtcag atatggacac aattaaagac
     1141 cgcgtcctgg atccccgccg acccccgttc gaacgcctcg tcgctctcct cgctatcctc
     1201 cgctctcccg agggatgcgc ctgggaccgc aagcagaccc atcgcagcct cctgccctac
     1261 ctgatcgagg aaacctacga ggtggtcgag gccgtcgaaa acgacaaccc ggccgccctc
     1321 cgcgaggagc tcggcgatct cctcgttcag attgtcttcc acgcccagct cgccgccgaa
     1381 gccggccggt tcactatcga caactcgatc gccgacgtgg tcgagaaact ggtcgcccgc
     1441 cacccccatg tgttcggcga acgaaaaaac ctcaatcccc gccaggtacg cgaccagtgg
     1501 gaacagatca aagtcgagtc gggggagaag gaccgggtcc tctccggcgt ccccaaagcc
     1561 atgcccgccc tgactatggc ctaccgggtg ggcgagaaag ccggcggtgt cgggttcgac
     1621 tggaacagcc ccgaagaggt gttgggcaaa atagaggagg agattggcga aatccgcg
//
LOCUS       DAOOKS010000497         1677 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_93105_length_1677_cov_2.303329, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000497 DAOOKS010000000
VERSION     DAOOKS010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93105_length_1677_cov_2.303329"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="PK112_07850"
     CDS             <1..670
                     /locus_tag="PK112_07850"
                     /inference="COORDINATES: protein motif:HMM:NF017038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPC11996.1"
                     /translation="AVGFVFHRDGEMLAAETLFECAKSGDYSTARLKAYAERFERSWA
                     GAELRKTRNFHAGFEGGLYAGMFHAAVQTVTGGRGLFDRRVKKPDHEYMLTLAGFERR
                     FGRRPEKARVKCDNAYTFDKLTDVYKSGTMHEEEQPPHLVIADYDICNNRCPEEYGNP
                     CQHFCPAQVYEMVDDDQRPGKVRLQLTPSNCVHCKTCDIADPYQIIRWMTPQGGEGPN
                     YENL"
     gene            695..961
                     /locus_tag="PK112_07855"
     CDS             695..961
                     /locus_tag="PK112_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11997.1"
                     /translation="MTALVPGSAGVALLLAAFVLSLLGRLSEHGIAYLAMNALGAALA
                     CWYAWASGLLPFVVLEGAWAAAALVRLAVGTGKKRGSPQTGEPR"
     gene            complement(984..>1677)
                     /locus_tag="PK112_07860"
     CDS             complement(984..>1677)
                     /locus_tag="PK112_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC11998.1"
                     /translation="IGFFKAAEDHQFPVRNTGLFFRGRNIQYLGSDAQAGMFAFDEAV
                     DAISDVWENNSAIYVVQVAEKRPAGQATFEEARERVTLDVQQEKVMALCVDTAAAIWN
                     LIQQGSDPKTAAERFGEQYETPDPFTRSGYAPGIRRDPLAIGAAFGLSRPGEFSGPVK
                     YDQGVVIFRLLSKEAPDMSVYTEKRDSVRTVVLNNKRQELFTRWMEETVKSSKVENYV
                     QRALVEQRGMPY"
BASE COUNT          282 a          530 c          586 g          279 t
ORIGIN      
        1 tgcggtcggc ttcgtattcc atcgcgacgg cgagatgctc gcggccgaga cgctcttcga
       61 atgcgcgaag agcggggact attcgacggc gcggctcaag gcatacgcgg agcggttcga
      121 gcggagctgg gccggggcgg aactccgcaa gacgcgcaat ttccacgcgg gcttcgaggg
      181 cgggctctac gccggaatgt tccacgccgc ggtgcagacg gtgaccgggg ggcgcgggct
      241 gttcgaccgc cgcgttaaaa aaccagacca cgagtacatg ctcaccctcg ccggattcga
      301 acgccggttc gggcggcggc cggagaaagc gcgcgtgaag tgcgacaacg cctacacctt
      361 cgacaaactc accgacgtgt acaagtcggg gacgatgcac gaggaggagc agccgccgca
      421 cctggtgatc gccgactacg acatctgcaa caaccggtgc cccgaggagt acggcaaccc
      481 ctgccagcac ttctgtcccg cgcaggtgta cgagatggtg gacgacgacc agcggccggg
      541 aaaagtgcgg ctccagctca cgccctcaaa ctgcgtccac tgcaagacct gcgatatcgc
      601 cgatccctac cagattatcc gctggatgac gccgcaggga ggcgaggggc cgaactacga
      661 gaatctctga gggccgcggg atgacggatc gcgcatgacg gcgttggtgc cggggtcggc
      721 cggcgtggcc ctcctcctgg ccgccttcgt gctgagtctg ctggggcggc tgtcggagca
      781 cgggatagcc tatctggcga tgaatgcgct gggcgcggcg ctcgcctgct ggtatgcctg
      841 ggcgagcggt ctgctgccgt tcgtggtcct cgagggggcc tgggcggcgg cggcgctcgt
      901 gcggctggcg gtcgggaccg gcaaaaagag aggctcgccg caaacgggcg agcctcgcta
      961 atcggcggcg ccggggtcga aactcagtag ggcatgcccc gctgctcgac cagcgcgcgc
     1021 tggacgtagt tctccacttt cgaactcttc accgtctcct ccatccaccg cgtaaacagc
     1081 tcctgccgct tgttgttgag gacaacggtg cggacggagt cgcgcttctc cgtgtacacc
     1141 gacatgtcgg gagcctcttt gctgaggagc cggaagatca cgaccccctg gtcatacttg
     1201 accggtccgg agaactcgcc cggccgactc aggccgaatg cggccccgat cgccagcgga
     1261 tcgcggcgga tgccgggcgc gtagccggag cgggtgaagg ggtcgggggt ttcgtactgc
     1321 tcgccgaagc gctcggcggc ggtcttcggg tcgctcccct gctggatcag gttccagatg
     1381 gcggccgcgg tgtcgacgca cagcgccatg actttctcct gctgcacgtc gagagtgacg
     1441 cgctcgcggg cctcctcgaa cgtggcctgc ccggccggac gcttctcggc gacctggacg
     1501 acataaatgg cgctgttgtt ttcccagaca tcgctgatgg cgtcgacggc ttcatcgaag
     1561 gcgaacatgc cggcctgcgc atcgctgccg aggtactgaa tgttgcgccc gcggaagaaa
     1621 aggccggtgt tgcgcacggg gaactggtgg tcctcggcgg ccttgaagaa gccgatc
//
LOCUS       DAOOKS010000498         1671 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_93594_length_1671_cov_3.211015, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000498 DAOOKS010000000
VERSION     DAOOKS010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93594_length_1671_cov_3.211015"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="PK112_07865"
     CDS             complement(<1..566)
                     /locus_tag="PK112_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HPC11999.1"
                     /translation="MCGIVGYVGPKQAKPILLQGLKRLEYRGYDSSGIALLTERGLMV
                     AKSAGKIANLEKMLSENEFDSSLCTHGIAHTRWATHGEPNQLNAHPHTDGRGEIALVH
                     NGIIENYRALKEFLQRQGFELKTETDTEVLVQLIRYYYRDDLTEAVCEALAQVEGTYG
                     IAVISARHPNTIVAARQGSPLVLGIGEK"
     gene            complement(573..>1671)
                     /locus_tag="PK112_07870"
     CDS             complement(573..>1671)
                     /locus_tag="PK112_07870"
                     /inference="COORDINATES: protein motif:HMM:NF014879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HPC12000.1"
                     /translation="AGGICITASHNPSPWNALKFFNRTGEFITPAQFRELDELFRSGR
                     FGYRPYDRLGSIDRQEGWIDWHIQKTLAVPFVNRAAVRRRKFRVVVDAINGAGSTALP
                     ALLRKLGAGVVEVNCKGDGDFVHEPEPIPKNLRQLSLMVRKHKADIGMACDPDADRLA
                     LVDERGRPIGEELTLALSVQQVLTRKKGPVVINLSTSKVTERVARDRGAAVYYAKVGE
                     ANVVELMRAKRALVGGEGNGGVILPSFHAGRDALMAAALVLSRLAEANVEFSSLADSL
                     PTYYTRKDKGTVPADFSARLARFEREAPGLLGEFAIDRQDGLRVDFSGGWVQIRTSNT
                     EPIFRLIVETESKDRTEHLADRVMGYFREPR"
BASE COUNT          295 a          549 c          502 g          325 t
ORIGIN      
        1 tccttctcgc cgatgccgag cacgaggggg gagccctggc gcgcggcgac aatggtgttg
       61 gggtggcggg cggagatgac ggcgatcccg taggtccctt cgacctgggc gagcgcctcg
      121 cacaccgcct cggtgagatc gtcgcggtag tagtagcgga tcagctggac gaggacctcg
      181 gtgtcggtct cagtcttgag ttcgaatccc tggcgctgga ggaattcttt gagggcgcgg
      241 tagttttcga tgatcccgtt gtggacaagg gcgatttcgc cccggccgtc ggtgtggggg
      301 tgggcgttga gctggttggg ttccccgtgg gtggcccagc gggtgtgggc gatgccgtgc
      361 gtgcagaggc tgctgtcgaa ctcattctcg ctgagcatct tctccaaatt ggcgatcttc
      421 cccgccgact tcgccaccat caggccgcgc tcggtcaaca gggcgatccc cgacgaatcg
      481 tatccccgat actccaggcg cttcaacccc tgcaggagaa tcggcttggc ctgcttcggc
      541 cccacgtaac ccacgatacc gcacataggc tcttacctcg gctcgcgaaa atatcccatc
      601 acccggtctg ccaggtgttc agtcctgtcc ttgctttccg tttctacaat caggcggaaa
      661 atcggctcag tattcgaggt gcggatctgg acccatccgc ccgagaaatc cactcgcaat
      721 ccgtcctgtc gatcgatggc aaattccccg agcagtcccg gcgcctcccg ctcaaaccgg
      781 gcgagccgcg cagagaagtc ggcgggtacg gtccccttgt cctttctagt ataataggtc
      841 ggaagggaat cggcaagact tgaaaactcc acgtttgcct cggcgagacg ggacagcacc
      901 agggcagccg ccatgagggc gtcccggccg gcatggaacg acggcaaaat cactcccccg
      961 ttcccctcgc ctccgaccag cgcccgtttc gcccgcatga gctcgacgac attcgcttcg
     1021 cccactttcg catagtacac cgctgcgccc cggtcgcggg cgacccgttc ggttactttc
     1081 gaggttgaca aattgatgac gaccgggccc tttttccggg tcaggacctg ctgaaccgaa
     1141 agagccagag tcagttcctc gccgatcggg cgtccccgct catcgaccag cgccagccgg
     1201 tcagcatcgg ggtcgcaagc cattcctatg tccgccttat gcttacggac cataaggctg
     1261 agctgccgca aattcttggg aatcggctcc ggttcatgga caaagtcgcc gtcccccttg
     1321 cagttaacct cgaccacccc ggcccccagc tttcgcagca gggcgggcag tgccgtcgag
     1381 cctgcaccat taatagcgtc caccaccacc cggaacttcc ggcgccgaac cgccgcccga
     1441 ttcacgaatg gtaccgccag agtcttctgg atgtgccagt cgatccaacc ctcctgccgg
     1501 tcgattgacc ccaggcggtc atacggccgg tagccgaaac gccccgaccg gaaaagctcg
     1561 tcgagttccc ggaactgggc cggggtgatg aactcgccgg tccggttgaa aaacttcagc
     1621 gcgttccacg gcgacgggtt gtgggaggcg gtgatgcaga tgccgccggc c
//
LOCUS       DAOOKS010000499         1667 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_93987_length_1667_cov_2.423077, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000499 DAOOKS010000000
VERSION     DAOOKS010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93987_length_1667_cov_2.423077"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="PK112_07875"
     CDS             <1..589
                     /locus_tag="PK112_07875"
                     /inference="COORDINATES: protein motif:HMM:NF015113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HPC12001.1"
                     /translation="FAVDVWADLIVAEPGDACPSCGGALSAARGIEVSQVFQLGTKYS
                     DSLGAKFLDAEGKERPILMGSYGIGVTRTPQAALEKYHDERGICWPKNIAPYQVALIP
                     LNMDKAEHRTAAENIYTALNEANIDVLWDDRTERAGVKFNDADLIGLPVRLVIGDKSL
                     ADGKVEMKARRGGEPHLVPIDRIVPAVRDLLDTLP"
     gene            686..1141
                     /gene="rimP"
                     /locus_tag="PK112_07880"
     CDS             686..1141
                     /gene="rimP"
                     /locus_tag="PK112_07880"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HPC12002.1"
                     /translation="MPSVNERFKADLLALVEAPLAAEGAYVADLVVATFKKSATVRVF
                     VYSERGTTLEECARLSRIVGDVLDGTDWFDYGYTLEVSSPGLDRPLVTPRDFRFRTGE
                     TVRVEFRDPARKKLTAELVAATDTDVELRHEGTVERVPLAEIKRAKIVF"
     gene            1155..>1667
                     /locus_tag="PK112_07885"
     CDS             1155..>1667
                     /locus_tag="PK112_07885"
                     /inference="COORDINATES: protein motif:HMM:NF020118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NusA N-terminal domain-containing protein"
                     /protein_id="HPC12003.1"
                     /translation="MSFDMIEAMTLIAREKNIEFDAVLETLEAGLMAAAKKKYAFTDN
                     ITFRFDRKNNELLMIATKRVVEQVADPNVELTLAEAKDIDPEAELGDEVDIYIDYEVE
                     FGRNAIAAASRSSSRRSARPNATASTTSTSTRSAPSSPASSSRSTRAMSSSTSAAGRG
                     SCPSRSRFPVR"
BASE COUNT          335 a          518 c          509 g          305 t
ORIGIN      
        1 cttcgcggtc gacgtgtggg ccgacctcat cgtcgctgag cccggcgatg cgtgcccatc
       61 gtgcggcggc gccctctcgg ctgcgcgagg catcgaggtc tcgcaggtct tccagctcgg
      121 taccaagtac tccgattcgc tgggcgcgaa attcctcgat gcggagggga aggagcggcc
      181 tatccttatg ggctcctatg ggatcggggt cacccgcacc cctcaggccg ccctcgaaaa
      241 gtaccacgac gagcggggca tctgctggcc caaaaacatc gccccctacc aggtcgctct
      301 cattccgctc aatatggaca aggcggagca ccggacggcc gcggagaaca tctataccgc
      361 cttgaacgag gccaatatag atgtactttg ggatgatcgt acggagcggg ctggggtcaa
      421 gttcaacgat gccgacctga tcggcctgcc ggtacgcctc gtaattggcg ataagtcgct
      481 cgccgatggg aaggtagaga tgaaggcccg gcgcgggggg gagccccatc tcgtgcctat
      541 cgaccgcatc gtgccggctg tccgcgacct cctggacacc ctgccgtaaa tcgttccatt
      601 tttgggttgc gcggcaactc aagttcccat atcttaacag attctgggtg ggcagtggcc
      661 catttttttg tacgggaaac ggtgtatgcc gagtgtgaac gaacggttca aagccgacct
      721 cctggcgctc gtcgaggcgc cccttgccgc ggaaggcgcg tatgtggccg acctcgtcgt
      781 cgccactttc aagaagagtg ccaccgtccg cgtgttcgtc tactccgaac gggggacgac
      841 gcttgaggaa tgcgcccggc tctcgcggat cgtgggcgat gtgctcgatg gaaccgactg
      901 gttcgactac ggttatactt tggaagtgtc ctcgcccggc ctcgatcggc cgctggtgac
      961 cccgcgcgac ttccggttcc gcaccggcga gaccgtccgc gttgaattcc gcgacccggc
     1021 gcgcaagaag ctgaccgccg aattggtcgc ggcgaccgat acggatgtgg aactgcggca
     1081 cgaagggacc gtcgagcgcg tgcccctggc cgagatcaag cgggcgaaaa tcgtattttg
     1141 acggagatgc gtgtatgtcg tttgacatga tcgaggcgat gactctcatc gcccgagaaa
     1201 agaacatcga atttgatgcg gttctggaaa ccctcgaggc cggcctcatg gccgccgcca
     1261 agaagaagta cgccttcacc gacaacatca ccttccgttt cgaccgcaag aacaacgaac
     1321 tgctcatgat cgcaacgaaa cgggtcgtcg aacaggttgc cgaccccaac gtcgaactca
     1381 ctctcgccga ggccaaggac atcgatcccg aagccgagct cggcgacgag gtcgacatct
     1441 acatcgacta cgaggtggag ttcggccgca acgccatcgc cgccgcaagc agatcctcat
     1501 ccagaaggtc cgcgaggccg aacgcgaccg catctacgac gagtacatcg acaaggtcgg
     1561 caccctcatc tccggcgtcg tccagcaggt cgacaagggc aatgtcatcg tcaacctcgg
     1621 ccgcggggag gggatcatgc ccatcaagga gcagattccc cgtgaga
//
LOCUS       DAOOKS010000500         1666 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_94063_length_1666_cov_2.545003, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000500 DAOOKS010000000
VERSION     DAOOKS010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94063_length_1666_cov_2.545003"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..879
                     /locus_tag="PK112_07890"
     CDS             118..879
                     /locus_tag="PK112_07890"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HPC12004.1"
                     /translation="MFSLPEAMLRVVAEKTGRSLVDIYGVATFYRAFSLRPRGKHLVS
                     CCLGTACHVRGGPAIAREIEKRLGIRAGETTADREFTLETVNCLGACALGPIVVVDGH
                     YFSKVKTSMIPDILARAKEGLDAVRVETDERIFPVEVSCARCNHSLMDPRHEIEGHPS
                     VRVTASFGSKHSWLAISSLWGSYTVHSEHEIPAETVVTFFCPHCHAEMMGGAPCAECG
                     APMVPMIVRGGGIVQLCSRRGCKGHILDFGGTAAD"
     gene            914..>1666
                     /locus_tag="PK112_07895"
     CDS             914..>1666
                     /locus_tag="PK112_07895"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12005.1"
                     /translation="MARKLTSVDDLAGFRSRVMAERRRAEAIPTIVVSADTGAQASGV
                     NDLIRLFKKHMLDRFLQEKLRLRITGCHGFCEMNPYVTVEPYGHFYPRLRPADVPRII
                     EAALSGQPADDLLYRDAATGTVYPRQSDIPFFARQKRTIVGNNQKLDPIRIMDYFENG
                     GYGALLKALEKNDPEWIIQEVQTAGIRGRGGAGFPTGRKWALARTSGRPGQQKYIVCN
                     ADEGDPGAYMDRGVLEGNPHAIMEGMVIGGLAI"
BASE COUNT          318 a          429 c          643 g          276 t
ORIGIN      
        1 ttttttgaat aaaataaaaa aagtcatacc tttgcagctc ataaaatgtg tttggaaaga
       61 ggttatcttc ctcgacgaca aaatcatcca ttatggcttc aaacattaaa tgtataggtg
      121 ttctcacttc cggaggcgat gctccgggtg gtggcggaga agacgggccg gtcgctggtc
      181 gacatctacg gggtggcgac gttttaccgg gcgttcagtt tgaggccgcg ggggaagcac
      241 ctggtgtcgt gctgtctggg gacggcgtgc cacgtgcggg gggggccggc gattgcgcgg
      301 gagatcgaga agcggctggg gatcagggcg ggggagacga cggcggaccg ggagttcacg
      361 ctggagacgg tgaactgcct gggggcatgc gcgttggggc cgatcgtggt ggtcgacggg
      421 cactactttt cgaaagtgaa gacatcgatg atcccggaca ttctcgcgcg ggcgaaggag
      481 gggctcgacg cagtccgggt ggagacggac gagcggatct tcccggtcga ggtgagctgc
      541 gcgcggtgca accacagtct gatggatccg cggcacgaga tcgaggggca cccgtcggta
      601 cgggtgacgg cctcgttcgg cagcaagcac agctggctgg cgatatcgtc gctgtgggga
      661 tcgtacacgg tgcactcgga gcacgagatt ccggcggaga cggtggtgac gtttttctgt
      721 ccgcactgcc atgcggagat gatgggcggg gcgccctgcg cggagtgcgg ggcgccgatg
      781 gtgccgatga tcgtgcgcgg cggcggtatc gtgcagctct gctcgcggcg gggctgcaag
      841 ggacacatcc tcgatttcgg cggcacggcg gccgactgac ggaggcaatg cgatgaacga
      901 ggagacggca taaatggcaa gaaaactgac atcagtcgat gacctggcgg gattccggag
      961 tcgggtgatg gcggagcggc ggcgggcgga ggcgattccg acgatcgtgg tgagcgccga
     1021 cacgggcgcc caggcgagcg gcgtgaacga cctcatccgg ctgttcaaga agcacatgct
     1081 cgaccggttc cttcaggaaa aactgcgcct gcgcatcacg gggtgccacg ggttctgcga
     1141 gatgaacccc tacgtgacgg tggagccgta cgggcacttc tatcccaggc tgagaccggc
     1201 ggacgtgccg cggataatcg aggcggcgct gagcgggcag ccggccgacg acctgctcta
     1261 ccgcgacgcg gcgaccggga cagtgtaccc gcggcagagc gacattccgt ttttcgcgcg
     1321 gcagaagcgg acgatcgtgg gcaacaacca gaagctcgac ccgatccgga tcatggatta
     1381 tttcgagaac ggcgggtacg gggcgctgct gaaggcgctg gagaagaacg acccggagtg
     1441 gatcatccag gaggtgcaga cggcggggat ccgcgggcgc gggggggccg gtttcccgac
     1501 cgggcggaag tgggcgctgg cgcggacctc ggggcggccg gggcagcaga aatacatcgt
     1561 gtgcaacgcc gacgaggggg atccgggagc gtacatggac cgcggcgtgc tagaggggaa
     1621 tccgcacgcg atcatggagg ggatggtgat cggggggctg gcgatc
//
LOCUS       DAOOKS010000501         1663 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_94298_length_1663_cov_4.562189, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000501 DAOOKS010000000
VERSION     DAOOKS010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94298_length_1663_cov_4.562189"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1485)
                     /gene="atpA"
                     /locus_tag="PK112_07900"
     CDS             complement(<1..1485)
                     /gene="atpA"
                     /locus_tag="PK112_07900"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008339891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HPC12006.1"
                     /translation="MGLNPEEVTSVIKKELEKYETRLEMESVGTVLQVGDGIARIWGL
                     EDVQMSELIEFPGDIMGLVLNLEEDNVGAAIFGSDTHIHEGDTVRRTGRVASVPVGEA
                     LLGRVVNPLGQPLDGKGPIVTDKFRVIEGHAPGVVDRQPVKEPLQTGLKAIDSMIPIG
                     RGQRELVIGDRQTGKTALVIDTIINQRHSDVYCIYVAIGQKASTVAQVVETLRRYGAM
                     EYTTVVAATATDPAPLQYIAPYAGCAMGEEFMYNGKHALVVYDDLSKQAQSYRQLSLL
                     LRRPPGREAYPGDIFYTHSRLLERAAKLNEKHGGGSLTALPIIETQAGDVSAYIPTNV
                     ISITDGQIFLEGELFFAGVRPAINVGISVSRVGGNAQTKMMKQVAGSLKLDLAQFREL
                     EAFTQFGSDLDEATLRQLNRGRKMVELLKQGQYVPMSVEHQVMIIWVGTKGYLDDVPA
                     EAVKTFEAEFLAFCEKEFPDIAHNLSKEKKISESTEKELQAAVAK"
BASE COUNT          249 a          561 c          528 g          325 t
ORIGIN      
        1 cttcgccacc gccgcctgca gctctttctc cgtcgactcc gaaatcttct tctctttgct
       61 caggttgtgg gcgatgtccg ggaattcttt ctcgcagaag gcgaggaatt cggcctcgaa
      121 cgtcttgacc gcctcggccg gcacgtcgtc gagatacccc ttggtcccca cccagatgat
      181 catcacctgg tgctcgaccg acatcggcac gtactgcccc tgtttgagga gctcgaccat
      241 tttgcgcccg cggttgagct ggcggagcgt cgcctcgtcg aggtccgacc cgaactgggt
      301 gaaggcctcg agctcgcgga actgggcgag atcgagcttg agcgacccgg ccacctgctt
      361 catcatcttg gtctgggcgt tgccgcccac gcgggacacc gagatgccca cgttgatggc
      421 ggggcggacg ccggcaaaga acagctcgcc ctcgaggaaa atctggccgt cggtgatcga
      481 aatcacgttg gtcggaatgt aggccgacac gtcgccggcc tgcgtctcga taatgggcag
      541 ggccgtgagc gacccgccgc cgtgtttttc gttcagcttg gcggcgcgct cgagcagccg
      601 cgagtgggtg tagaagatgt cgccggggta ggcttcgcgg cccggcgggc ggcgcagcaa
      661 gagcgacagc tggcggtacg actgggcctg cttggacagg tcgtcgtaca ccaccagggc
      721 gtgcttgccg ttgtacatga actcctcgcc catggcgcag ccggcgtagg gggcgatgta
      781 ctgcagcggg gccgggtcgg tggcggtcgc cgccacgacc gtcgtgtact ccatcgcccc
      841 gtacctgcgc agcgtctcca ccacctgcgc caccgtcgag gccttctggc cgatcgcgac
      901 gtagatgcag tagacgtcgg aatggcgctg gttgatgatc gtgtcgatca cgagcgccgt
      961 cttgccggtc tggcggtcgc cgatcacgag ctcgcgctgg ccgcgcccga tcgggatcat
     1021 cgagtcgatc gccttcaacc ccgtctggag cggttctttc accggctggc ggtcgaccac
     1081 gcccggcgcg tggccctcga tcacgcggaa cttgtcggtg acaatcggcc ccttgccgtc
     1141 gagcggctgg ccgagcgggt tcaccacccg ccccagcagg gcctcgccga ccgggacgct
     1201 ggcgacgcgg ccggtccgcc gcaccgtgtc cccctcgtga atgtgcgtat cggagccgaa
     1261 gatggcggcg ccgacattgt cttcttcgag attgagcacg aggcccatga tgtcgccggg
     1321 aaactcgatc agctcggaca tctggacatc ctcgagcccc cagatgcgcg cgatcccgtc
     1381 gcccacctgg agtacggttc cgacggattc catttcgagc ctggtttcgt acttctccag
     1441 ctctttcttg atgaccgatg taacttcttc agggttgaga cccatcttcg tactcctgtc
     1501 ttaaccgcac cgccatcgcc gcatccgggc ggggccgcgc cgccgccccg cgcgcgtcgc
     1561 atggggcggc gcctgcggcg gtcagttcac tttcaccttc aaaagctgct cctcgatcag
     1621 ccccagcccg tgccggatcg agccgtcgat aatctcgttg tgc
//
LOCUS       DAOOKS010000502         1660 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_94620_length_1660_cov_2.640498, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000502 DAOOKS010000000
VERSION     DAOOKS010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94620_length_1660_cov_2.640498"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1411)
                     /gene="uvrB"
                     /locus_tag="PK112_07905"
     CDS             complement(<1..1411)
                     /gene="uvrB"
                     /locus_tag="PK112_07905"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HPC12007.1"
                     /translation="MAKPTAPTAAGHTPFRLRSQFSPRGDQPQAIAQLLAGLREGRMH
                     QVLLGVTGSGKTFTIANVIAQYGRPALVLSHNKTLAAQLYGELTAFFPDNAVEFFISY
                     YDYYQPEAYLPTTDTYIEKDTSINEDIDRLRLRATASLLERQDVIIVASVSCIYGLGS
                     PVEYKNQYIPLVRGGEYNRDQVIRKLIDIHYNRNDIDFSRGNFRVRGDTVELIPAYHD
                     HAMRIEFFGDEVERITEIDPLTGEVLRERDRIAIYPAKHFITSPERMQEAIRGIREEL
                     AERLEFFRSRNLLLEAQRLEMRTKYDLEMLEEVGYCSGVENYSRHLSGRRPGERPFTL
                     LDFFPDDFLTIIDESHQTIPQVRGMFAGDRSRKEVLVEHGFRLPSALDNRPLVFEEFE
                     RVVLKRVYVSATPADYELTKAEGIVVEQVIRPTGLLDPVITVRPLGTQVDDLLAEARR
                     RAERGERVLVTTLTKRMAED"
BASE COUNT          271 a          491 c          580 g          318 t
ORIGIN      
        1 ggtcctcggc catgcgtttg gtgagcgtgg tgaccagcac gcgctcgccg cgctccgccc
       61 gccgcctggc ctcggccagc aggtcgtcga cctgcgtccc gagcgggcgg acggtgatga
      121 cggggtcgag cagaccggtc ggccggatga cctgctcgac cacgatcccc tccgccttgg
      181 tcagttcgta gtcggccggg gtcgcggaca cgtacacccg cttcagcacc acccgctcga
      241 actcctcgaa tacgagcggc cggttatcca gcgccgaggg caggcggaaa ccgtgctcga
      301 cgagaacctc cttgcggctg cggtcgccgg cgaacatccc ccgcacctgg gggatggtct
      361 ggtgcgactc gtcgataatg gtcaggaagt cgtcggggaa gaaatccagc agggtgaagg
      421 ggcgctcgcc cggccggcgc cccgacaggt gccgggagta gttctcgacc ccggagcagt
      481 acccgacctc ttcgagcatc tcgagatcgt acttggtgcg catctccagc cgctgggcct
      541 ccaagagcag gttgcgggag cggaagaact ccagccgctc ggccagttct tcgcggatgc
      601 cgcggatcgc ttcctgcatc cgctcgggcg aggtgatgaa gtgtttggcc gggtagatgg
      661 cgatccggtc gcgctcgcgc agcacctccc ccgtcagcgg atcgatctcc gtgatccgct
      721 cgacttcgtc gccgaaaaac tcgatccgca tggcgtggtc gtggtaggcc gggatgagtt
      781 cgacggtgtc gccgcggacg cggaaatttc cccgcgagaa gtcgatgtcg ttccggttgt
      841 agtggatgtc gatgagcttg cggatgacct ggtcgcggtt gtactccccc ccgcgcacga
      901 gcgggatgta ctggttcttg tattcgaccg gcgacccgag gccgtaaatg caggagaccg
      961 aagccacgat gatgacgtcc tggcgctcga gcagcgaggc ggtcgcgcgc agccgcagcc
     1021 ggtcgatatc ctcgttgatc gaggtgtcct tctcaatgta cgtgtccgtg gtcggcaggt
     1081 acgcctccgg ctggtagtag tcgtagtagc tgatgaagaa ctcgacggcg ttgtcgggga
     1141 agaaagccgt gagctcgccg tagagctggg cggcgagcgt cttgttatgc gacagcacga
     1201 gcgcgggacg cccgtactgg gcgatcacgt tggcgatggt gaacgtcttt ccggaaccgg
     1261 tgacgccgag cagcacctgg tgcatgcggc cttcccggag ccccgcgagc agttgggcga
     1321 tcgcctgggg ctggtcgccg cggggcgaga actggctgcg gagccggaac ggggtgtggc
     1381 cggcggcggt cggggcggtt ggtttggcca tgtggttcat cgtcctccac tgacgcaggt
     1441 gcgccgcaat atatgcaata caaccgaggg ggcaatggcg attgttcgcg gccggccctg
     1501 gtgccggtcg tccacaattg gaacccgttg ggcgaccgcg ggtttaaaag gtgatttgga
     1561 cgtcttcgac gcaagtttgg ccttgacttg gcgtgcaaaa ggtatacttt atgcggttta
     1621 acctgatttg ggataacagc tttccacgtt tggaggaatg
//
LOCUS       DAOOKS010000503         1660 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_94631_length_1660_cov_2.292212, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000503 DAOOKS010000000
VERSION     DAOOKS010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94631_length_1660_cov_2.292212"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1152
                     /gene="tyrS"
                     /locus_tag="PK112_07910"
     CDS             <1..1152
                     /gene="tyrS"
                     /locus_tag="PK112_07910"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HPC12008.1"
                     /translation="KRSLRENKPLRVKQGFDPTAPDIHLGHTIGIRKLRQFQDLGHTV
                     VLIVGDYTGMVGDPSGRSATRPQLDYDTLMKNAETYQRQFFKILDRAKTEVHFNGEWF
                     KQMSFAEVMQLAMKFTVARLLERDDFEKRMRRGIPISVHELFYPLMQAYDSVAIRADV
                     ELGATEQKFNLLAGRTIQEAYGVEPQCILTLPVLVGIDGENRMSKSLGNYIGIDESPR
                     EIFGKIMKIPDRLIADYAALVADYTLEELEDIKQKLASPDINPMDVKKMLGERMVEMY
                     HQAGAGAAAREEFGRVFSQKQLPDEIPECPSARIREWGLDPDRIYLVHLMAKAELAKS
                     NSDARKLIQAGAVELDGERVSDPDYEFALPDTGFVVLKVGKRRFLRLRR"
     gene            1244..>1660
                     /locus_tag="PK112_07915"
     CDS             1244..>1660
                     /locus_tag="PK112_07915"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPC12009.1"
                     /translation="MIRIVFTATVAAALLGGCASQAIRRETVEVHADPQPVRVEKIPL
                     AAEIERVNPLAYNFFVDGVLFEALGNSYQATESYGKALQYYPDSYVIRASLAENLFRM
                     QRFDDALAVLQPVAPEDAAVWELRGDIYRAGGQIDAS"
BASE COUNT          320 a          492 c          549 g          299 t
ORIGIN      
        1 aagcggtcgc tccgcgagaa caagccgctg cgggtgaagc aggggtttga cccgacggcg
       61 cccgacattc acctcggcca caccatcggc atccgcaagc tgcggcagtt tcaggacctc
      121 ggacacaccg tcgtgctcat cgtcggcgac tacaccggca tggtcggcga cccctcgggg
      181 cggtcggcca cccggccgca gttggactac gataccctga tgaagaacgc ggagacttac
      241 cagcggcagt ttttcaagat tctcgaccgc gcgaaaaccg aggtgcattt caacggcgag
      301 tggttcaagc agatgtcgtt cgccgaggtg atgcagctgg ccatgaagtt cacggtggcc
      361 cggctcttgg agcgggacga tttcgaaaag cgaatgcggc gcgggattcc gatctcggtc
      421 cacgagctct tctacccgct catgcaggcg tatgattcgg tggcgatccg ggccgatgtc
      481 gagctcggcg cgaccgagca gaagttcaac ctgctggccg gaaggacgat ccaggaggct
      541 tacggcgtcg agccgcagtg catcctcacc ctgccggtgc tggtggggat cgacggcgag
      601 aaccggatgt ccaagtcgct gggaaactat atcggaatcg acgagtcgcc gcgggagatt
      661 ttcggcaaga tcatgaagat tcccgaccgg ctgatcgccg actacgccgc gctggtggcc
      721 gactacaccc ttgaggaact cgaggacatt aaacaaaaac ttgcaagtcc cgatattaat
      781 ccgatggatg tcaaaaagat gcttggggaa cgcatggtcg agatgtacca ccaggccgga
      841 gccggcgcgg cggcccggga ggagttcggg cgcgtcttct cccagaagca gttgcccgat
      901 gagattcccg aatgtccctc ggcccgcatc agggagtggg gacttgatcc cgaccgaatc
      961 tatctggtgc acctgatggc caaggccgag ttggcgaagt cgaacagcga cgcccgcaaa
     1021 ctgatacagg ccggagcggt cgagctcgac ggggagcggg tgtcggatcc cgactacgag
     1081 ttcgcgctgc ccgacaccgg cttcgtcgtg ctgaaagtag gcaaacgccg gttcctcagg
     1141 ctgcggcgat agcgaccggg cactttctac ggcggacgtt gtagtaaggg tgacctttcg
     1201 ctctccccgg gggcggcgtc cggtcccccg gagacggttc aagatgataa ggatagtttt
     1261 cacagcaacg gtggctgcgg cccttctcgg cggctgcgcc tcgcaggcga ttcgccgcga
     1321 gacggtcgag gtgcatgccg acccgcagcc ggtgcgggtg gagaaaattc cgctggcggc
     1381 ggagatcgag cgggtcaacc cgcttgctta caactttttt gtcgacggcg tgctgttcga
     1441 ggccctcggc aattcgtacc aggcgaccga gagttacggc aaagccctcc agtactaccc
     1501 ggactcgtat gtgatccgcg cctcgctggc tgagaacctc tttcgcatgc agcgtttcga
     1561 cgatgcgctg gcggtgctgc agccggttgc gccggaagac gccgcggtgt gggaattgcg
     1621 cggcgacatc tatcgcgcgg gggggcagat cgacgcctcg
//
LOCUS       DAOOKS010000504         1656 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_94952_length_1656_cov_4.634603, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000504 DAOOKS010000000
VERSION     DAOOKS010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94952_length_1656_cov_4.634603"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="PK112_07920"
     CDS             <1..387
                     /locus_tag="PK112_07920"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPC12010.1"
                     /translation="FHREIYRRQRAREDAAPRRSFITDRGTADAFAFHPETAAEVGTT
                     PAAEYRRYTGVIQLGTAAALGPEYYQADEVRIEPIAAVLALERQIRSVWERHPGYQFV
                     PARESVDEKYAVFVEIVCRLAGKTPV"
     gene            457..>1656
                     /locus_tag="PK112_07925"
     CDS             457..>1656
                     /locus_tag="PK112_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12011.1"
                     /translation="MRLPLVMAALLLWSGAPAADREFFKEFESTYDLLHVELLNNAGE
                     KAEVSNFVYQKDVATFTFEKGTVYLQRYVKDRPTTAVFVGQGRVTIDVPVHSERQGLW
                     AVTKDSTVDQPVEVVAIRMADDLDLRLRERFQFEPAVIDFRVVNKLGQGEFFFKPVIM
                     HTYDNYFRLAASIYERGPDGFFYADFNRYTFTFDPNWPEQVQIGYEVEGGDQVSTVGA
                     SFQRREAGRYDDSLLSQIAYQTTALRRTAEVRLSGTDGRRIEQAKATLDLAVNADSLR
                     FTWLFLKYSLDVDSVLVGDERIDYVRRKTFDYTGIILPRYYHQGDTLSLTVWYRGTNF
                     DHFLPYLSNPVPSPTEVTFNVPKGYNYYTAGMSEPVDVGGGRQEFTSALPAPLGELYF
                     HCFPTGVE"
BASE COUNT          305 a          510 c          551 g          290 t
ORIGIN      
        1 ttccaccgcg agatctatcg ccgccagcgg gcccgcgagg acgccgcgcc gagacggtcg
       61 ttcatcaccg accgcggcac ggccgacgcc ttcgccttcc acccggagac ggctgcggag
      121 gtcggcacga cgccggccgc cgagtaccgg cggtacaccg gggtcatcca gctcggcacg
      181 gccgccgcgc tggggccgga gtactaccag gcggacgaag tccgcatcga gccgatcgcg
      241 gcggtgctgg ctctcgagcg gcagatccgg tcggtctggg agcggcaccc gggttaccaa
      301 tttgtcccgg cgcgggaatc ggtggacgaa aaatacgcgg ttttcgtgga gatcgtgtgc
      361 cggctggccg gaaaaacacc ggtttaattt gacagcgggg gggatcctgg cgtttataag
      421 atactgaaag acagggacta aaatgtcgag ggagctatga gattaccgct cgtgatggcc
      481 gctctgctgc tgtggtccgg cgcgcccgcc gccgaccggg agttcttcaa ggagttcgaa
      541 tccacctacg acctcctcca cgtggagctg ctcaataacg ccggcgagaa ggccgaggtc
      601 agcaatttcg tctatcagaa agacgtcgcc acgttcacat tcgagaaagg gacggtgtat
      661 ctccagcggt atgtgaaaga ccgcccgacc acggccgtgt ttgtgggcca gggacgcgtc
      721 acgatcgacg ttccggtgca ttcggaacga caggggctgt gggccgtgac caaggacagc
      781 acggtggacc agccggtcga ggtggtggcg atccggatgg cggatgatct cgatctgcgg
      841 ctgcgggaga gattccagtt tgagccggcg gtcattgatt tccgcgtcgt gaacaagctt
      901 ggccagggcg agttcttctt caaaccggtg atcatgcaca cctacgacaa ttatttccgc
      961 ctcgccgcat cgatctatga acggggcccc gacgggttct tctatgccga tttcaaccgc
     1021 tacacgttca cctttgaccc caactggccg gagcaggtgc agatcgggta cgaggtggag
     1081 gggggcgacc aggtgtcgac cgtgggggcg agcttccagc gccgggaggc gggccggtac
     1141 gacgactcgc tgctgtcgca gatcgcgtac cagacgacgg cgctccgccg cacggccgag
     1201 gtgcggctgt cggggaccga cggccgccgc atcgagcagg cgaaagcgac gctcgacctg
     1261 gcggtcaacg ccgacagcct ccgcttcacc tggttgttcc tcaaatactc actcgacgtg
     1321 gattccgtgc tggtcgggga cgagcgcatc gactatgtgc ggcggaagac gttcgactac
     1381 acgggcatta tcctcccccg ctactaccac cagggggata cgctcagcct gacggtgtgg
     1441 taccgcggca cgaacttcga ccacttcctg ccctacctga gcaatcccgt tccgagcccg
     1501 acagaggtga cgttcaacgt ccccaagggc tacaactact acacggcggg gatgagcgag
     1561 ccggtggacg tcgggggcgg gcggcaggag ttcacctcgg ctcttccggc gccgctgggc
     1621 gagctgtact tccactgttt cccgaccggc gtggaa
//
LOCUS       DAOOKS010000505         1655 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_95099_length_1655_cov_2.623125, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000505 DAOOKS010000000
VERSION     DAOOKS010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95099_length_1655_cov_2.623125"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1655)
                     /gene="purL"
                     /locus_tag="PK112_07930"
     CDS             complement(<1..>1655)
                     /gene="purL"
                     /locus_tag="PK112_07930"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HPC12012.1"
                     /translation="LRFGLPDNPRVRYLVDGVVRGIGDYGNSFGVPTVAGEVCFDEAY
                     TGNPLVNAMAVGLVRADRIASAVARGAGNALMIVGSKTGRDGIHGATFASEELSEKSQ
                     EKRPSVQIGDPFTEKLLVEATLEIIEQDLIVGIQDMGAAGITCSSSEMSAKGKAGVEL
                     HIERVPVREPHMTPYEILLSESQERMLVCVKKGKEDAVRAVFDKWGLDSTIIGHVTDD
                     GLMTVRLDGQVVAQVPSRELVLGGGAPVYHRETRRPAYMDEIVRLDPRRYPLERDWNG
                     TLAAMLAAPNICHKGWVFEQYDSMVRTNTAVGPGSDAAVLRIRKTHKALAMTTDCNGR
                     YCYINPRLGAQSAVAEAARNIVCSGGQPLAITNCLNFGNPYKPEIYYGFAEAVAGMGE
                     ACRALGTPVTGGNVSFYNEDPRRAVFPTPTIGMIGLIEHTDHITTQWFKDEGDVVCLI
                     GRNSDHLGASEYLHTIFGETRGAVAPLDLQFERRMQEVLLAAIRAGEIKSAHDAADGG
                     LAVALAECCIARRERPIGAAITTGEDIRPDALLFGEAQSRVIV"
BASE COUNT          240 a          597 c          534 g          284 t
ORIGIN      
        1 cacgatcacc cgcgactgcg cctcgccgaa caggagtgcg tcggggcgga tgtcctcgcc
       61 ggtggtgatc gccgcgccga tcgggcgctc gcggcgggcg atgcagcact cggccagggc
      121 caccgccagc ccgccgtccg ctgcgtcgtg cgccgacttg atctcccccg cgcgaatggc
      181 cgccaggagg acctcctgca tccgccgctc aaactgcaga tcgagcggcg cgaccgcccc
      241 ccgggtttcg ccgaagatcg tgtggagata ctcgctcgcc ccgaggtggt cgctgttgcg
      301 gccgatcagg cagacgacgt ctccctcgtc tttgaaccac tgggtcgtga tgtggtccgt
      361 gtgctcgatc aggccgatca tcccgatcgt cggcgtcggg aacaccgccc gccggggatc
      421 ctcgttgtag aacgagacgt tccctccggt cacgggcgtg ccgagcgccc ggcaggcctc
      481 gcccatcccg gccaccgcct cggcgaaccc gtagtagatt tccggcttgt aggggttgcc
      541 gaaattgagg cagttggtga tcgccagcgg ctggccgccg gagcagacga tgttgcgggc
      601 cgcttcggcc accgcgctct gcgcgccgag gcgcgggttg atatagcagt agcgtccgtt
      661 gcagtcggtc gtcatggcga gcgccttgtg cgtcttgcgg atccgcagca cggcggcatc
      721 cgagcccggc ccgaccgccg tgttcgtgcg caccatcgaa tcgtactgct cgaacaccca
      781 ccccttgtgg cagatattcg gcgccgccag catcgccgcc agcgtcccgt tccagtcgcg
      841 ctccaaaggg taccggcgcg gatcgagccg cacgatctcg tccatgtagg ccgggcgccg
      901 cgtttcccga tggtacaccg gcgcgccgcc gccgagcacc agttcccggg acggtacctg
      961 ggcgactacc tgcccgtcca gccggaccgt catcagcccg tcgtcggtga cgtgcccgat
     1021 gatcgtcgaa tccagccccc acttgtcgaa gaccgcgcgc accgcgtcct ctttcccctt
     1081 cttcacgcac acgagcatgc gctcctgcga ttcggacagg agaatctcgt acggcgtcat
     1141 gtgcggttcg cgcaccggga cgcgctcgat gtgcagttcc acccccgcct ttcctttggc
     1201 cgacatctcc gaggagctgc aggtgatccc ggccgccccc atgtcctgga tgcccacgat
     1261 caggtcctgc tcgataatct cgagggtcgc ctcgaccagc agcttttccg tgaacgggtc
     1321 gccgatctgc accgaggggc gcttctcctg cgatttctcg gagagttcct cggaggcgaa
     1381 cgtcgcgccg tggattccgt cgcggccggt cttcgacccg acaatcatga gcgcgttccc
     1441 ggccccgcgg gcgaccgccg aagcgatccg gtcggctcgg accaggccga cggccatggc
     1501 gttgaccagc gggttgcccg tgtaggcctc gtcgaaacag acctcgccgg cgactgtcgg
     1561 gacgccgaac gaattgccgt agtcgccgat cccgcgcacg acgccgtcga cgagataccg
     1621 cacccgcggg ttgtccggga gcccgaagcg gaggg
//
LOCUS       DAOOKS010000506         1650 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_95538_length_1650_cov_2.991850, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000506 DAOOKS010000000
VERSION     DAOOKS010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95538_length_1650_cov_2.991850"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="PK112_07935"
     CDS             <1..296
                     /locus_tag="PK112_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-5'-phosphate-dependent protein subunit
                     beta"
                     /protein_id="HPC12013.1"
                     /translation="EGIGDDFLCPTLDLTVIDDICQVSDRDCFLMARDLTRKEGICGG
                     GSSGGIVLAALRYAQEADLAEDKLLVAILPDHGNKYISKIFNDEWMREKGFIE"
     gene            305..1483
                     /locus_tag="PK112_07940"
     CDS             305..1483
                     /locus_tag="PK112_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015481293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019343 - cysteine biosynthetic process via
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HPC12014.1"
                     /translation="MAHDRSKNWQFETTAIHSGRVPEDGTRSVTTPIYPSSTYRVDYP
                     GDESGYVYARWKNPTRLALEETLAKLERGTKAAAFASGLAALDAVLKLLKTGDHVVAV
                     DDLYGGTMRQFERIGRRFGLDFTYVDGRDPKNLESAIRPNTRLFWLETPTNPLMHLVD
                     IEKVAAVAKERGILVGVDNTFATPYLQQPLELGADIVLHSMTKYLGGHCDLVAGALIV
                     KDSSLGEQLWFNQYAGGAHLGPWESWLVLRGLKTLALRMERHSVNAMKIAEYLETVEA
                     VEKVYFPGLDGRPVPNRMRLPGGMVAFTIAPQFDFEAVKRFVMALRVFVLAESLGGVE
                     SLVNHPAAMTHSSIPREIREPRGITDGLVRLSVGIEHIDDLLIDLRSAFDALTQSARA
                     "
BASE COUNT          284 a          529 c          555 g          282 t
ORIGIN      
        1 tggaggggat cggcgatgac ttcctctgcc cgacgcttga tctgacggtg atcgacgaca
       61 tctgccaggt gagcgaccgg gactgtttcc tcatggcccg cgatctcacg cgcaaagagg
      121 gcatctgcgg cggcggttcc tccggcggga tcgtgctggc ggcgctccgc tacgcgcagg
      181 aagccgatct ggccgaggac aaactgctcg tggcgattct ccccgaccac gggaacaagt
      241 atatcagcaa aattttcaac gacgagtgga tgcgcgagaa aggattcatc gagtagaggc
      301 gcttatggct cacgaccgca gcaagaactg gcagttcgaa accaccgcga tccactccgg
      361 ccgcgtgccg gaggacggca cgcgctcggt gaccaccccg atctacccca gctcgaccta
      421 ccgtgtcgac tatccggggg atgagtcggg ctacgtgtac gcccgctgga aaaacccgac
      481 gcggctggcg ctcgaggaga cgctggcgaa actcgagcgg gggacgaagg cggcggcgtt
      541 cgcctccggg ctggccgcgc tcgacgcggt gctcaaacta ctcaaaaccg gcgaccacgt
      601 ggtggcggtc gacgatctct acggcggcac gatgcgccag ttcgagcgga tcggacggcg
      661 cttcgggctg gatttcacct atgttgacgg gcgcgacccg aagaatctcg agagcgccat
      721 ccgcccgaac acccggctct tctggctcga gaccccgacg aacccgctca tgcacctcgt
      781 ggacatcgag aaagtcgcgg cggtcgcgaa ggagcgcggg atcctggtcg gggtcgacaa
      841 cactttcgcc acgccctacc tccagcagcc cctcgaactc ggcgccgaca tcgtcctcca
      901 ctcgatgacc aagtacctcg gcggccactg cgacctcgtg gccggcgcgc tcattgtgaa
      961 ggattcctcg ctcggtgagc agctctggtt caaccagtac gccggcgggg cgcacttggg
     1021 accgtgggaa tcctggctcg tgctgcgggg gctgaaaacg ctcgcgctgc gcatggagcg
     1081 gcactcggtc aacgccatga agatcgcgga gtacctcgag acggtcgagg cggtggagaa
     1141 ggtgtatttt ccggggctcg acggccggcc ggtgccgaac cgcatgcgcc ttccgggcgg
     1201 catggtggcg ttcacgatcg cgccgcagtt cgatttcgag gcggtcaagc ggttcgtgat
     1261 ggcgctgcgg gtgttcgtgc tggccgagtc gctcggcggg gtggagtcgc tggtgaacca
     1321 cccggcggcg atgacccatt cctcgattcc cagggaaatc cgcgagccgc gggggatcac
     1381 cgacggcctg gtgcggctgt cggtcgggat cgaacatatc gatgatctgc tgatcgacct
     1441 gcggtcggcg ttcgacgcgc tgacgcagtc ggcccgggcc tgagcggccc gggcgggccg
     1501 gcggcgcgcc caatatctgc gcttgcctcg gcggccgggg atcatcttat tatctccgac
     1561 gaactcatac caccgaaagg agttgccccg tgcctcacaa aacgaccgtc gccgcgctcg
     1621 ttctcggcgg actggcgctg cttgttctcg
//
LOCUS       DAOOKS010000507         1639 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_96519_length_1639_cov_3.471591, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000507 DAOOKS010000000
VERSION     DAOOKS010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96519_length_1639_cov_3.471591"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="PK112_07945"
     CDS             <1..196
                     /locus_tag="PK112_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12015.1"
                     /translation="DNLAAGLGIDPENPDADRVRQAIEDTVQDVNNRVAAYKRITGCE
                     IREKELEKTSSKKVKRYLYQ"
     gene            217..633
                     /locus_tag="PK112_07950"
     CDS             217..633
                     /locus_tag="PK112_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008085364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HPC12016.1"
                     /translation="MVHAAEIALESAGFCDLVDITAQLSDLVRASGIGSGIVTVFCPG
                     STGGLTTIEFEPGLQKDLPELLERIVPSDRSYHHDRTWHDGNGFAHLRSALIGPDLTV
                     PFTAGKLALGTWQQVVFLDFDNKARRRRLAVQIIGE"
     gene            649..>1639
                     /gene="mtnA"
                     /locus_tag="PK112_07955"
     CDS             649..>1639
                     /gene="mtnA"
                     /locus_tag="PK112_07955"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019121350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HPC12017.1"
                     /translation="MAVEAIQRIGKKIRVIEQSKLPLAFSYLELDDYRDVIAAIRRLD
                     VRGAPAIGIAAAYALALAVEKAADFALSHLERAADEVKAARPTAVNLAWAVDRVMRRV
                     RQAPPLTRREALAILWEEAEAIHDEDRRLCERIGQYGADLIKPGDGILTHCNAGALAT
                     GGKGTALAVIYAAQEAGKRPRVYADETRPLLQGARLTAWELLQAGVDVTLICDSAAAT
                     VLRQGKVQHVIVGADRIARNGDAANKIGTYPLAVLAARHGVPFCVAAPYSTFDENTAA
                     GEQIPIEERAADEVTGGFGRRTAPEGVKVYSPAFDVTPFQLVTYYITDQGIKPG"
BASE COUNT          305 a          498 c          593 g          243 t
ORIGIN      
        1 cgacaacctt gccgccggac tcgggatcga tccggaaaac ccggacgccg accgggtgcg
       61 gcaggcgatc gaggacacgg tgcaggacgt caacaaccgc gtggcggcgt acaagcgaat
      121 caccgggtgc gagatacggg agaaagaact ggaaaagacg tcctccaaga aggtgaagcg
      181 gtacctgtac caatagggag agcgggcgga gatactatgg ttcacgcggc ggaaatcgcg
      241 ctggaatcgg ccgggttctg cgacctcgtc gatatcaccg cgcaactgtc cgatctcgtg
      301 cgcgcgtcgg gaattgggtc ggggattgtc accgtctttt gtcccggatc aaccggcgga
      361 ctgaccacca tcgaattcga accgggcctg cagaaggacc ttccggaact gctcgagcgg
      421 attgtgccgt cggaccggag ctaccaccac gaccggacct ggcacgacgg caacgggttt
      481 gcgcatcttc gctcggcgct catcgggccg gatctcaccg ttccgttcac ggcgggaaaa
      541 ctagcgctcg gcacgtggca gcaggtcgtg tttcttgatt tcgacaacaa ggcgcgccgg
      601 cggcggctgg ccgtgcagat tatcggcgag tagcgggggc ctgacaacat ggcggtagag
      661 gcgattcagc ggatcgggaa gaagatcagg gtgatcgagc aatccaagct cccgctggcg
      721 ttcagttacc tggagctgga cgactaccgg gacgtgatcg cggcgatccg gcggctcgat
      781 gtgcgcgggg cgccggcgat cgggatcgcg gccgcctacg cgctcgcgct ggcggtcgaa
      841 aaggcggcgg acttcgcgct ctcccacctc gagcgcgcgg ccgacgaggt gaaagcggcg
      901 cgcccgacag ccgtaaatct ggcctgggcg gtcgaccgcg tgatgaggcg tgtgcgccag
      961 gcgccgccgc tgacccgccg ggaggcgctc gcgatcctgt gggaggaagc ggaggcgatc
     1021 cacgacgagg accgccggct ctgcgagcgc atcgggcaat acggcgccga cctgatcaag
     1081 ccgggggacg gaatcctgac gcactgcaac gcgggcgccc tggccaccgg ggggaagggc
     1141 accgcgctcg cggtgatcta cgcggcccag gaggccggca aacggccgcg ggtgtatgcc
     1201 gatgagaccc ggccgctttt gcagggagcg cggctcacgg cctgggaact gctccaggcc
     1261 ggagtggacg tgactctcat ctgcgacagc gcggccgcga ccgtcctgcg gcaggggaaa
     1321 gtgcagcacg tgattgtggg ggccgaccgg atcgcgcgca acggcgacgc ggccaacaag
     1381 atcggcacgt atcccctggc cgtgctggcc gcccggcacg gcgtcccgtt ctgcgtggcg
     1441 gcgccgtatt cgacattcga tgaaaacacg gccgccgggg agcagatccc gatcgaggaa
     1501 cgggccgccg acgaagtgac gggcggattc ggccggagga ccgctcccga gggggtcaaa
     1561 gtgtattcgc cggcgttcga tgtgaccccg tttcagttgg tgacgtatta tattactgac
     1621 cagggaatca aaccggggg
//
LOCUS       DAOOKS010000508         1631 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_97244_length_1631_cov_2.733503, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000508 DAOOKS010000000
VERSION     DAOOKS010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97244_length_1631_cov_2.733503"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1631)
                     /locus_tag="PK112_07960"
     CDS             complement(<1..>1631)
                     /locus_tag="PK112_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12018.1"
                     /translation="PTARYNNTRPCNDSGEVVGFFETYEAPIGCGFRDTLNLAWHADR
                     DGNAYGGVFYDRPVGTQVDIADTMCMLASVRSGLGIVPLRGPVDRGGVYYNWWTSRWA
                     PSLGPIFMEPSADPSVRSFGRGSWWYNQDWFYSLESEYRRLSTHEQDHPNYWTGEIAR
                     ALTPPWNPVPLTEANAIASNTTPDYLVSWGPLTLAAGHKVTLSFAVVGGELIHKDPWI
                     LAYIPRRPAEYEQRLDFSDLIANARWAQRVFDNPGFDTDGDGYAGEFRVCVQDSALVG
                     GQWVAQKADTQWYKGDGIPDFRVAYPPPPPPFWLTPVPGGVHVRFNGQVSETQVDPLL
                     QAPDFEGYRIYLGRDERAEALTVVGSFDRRNYDKWVKKPDGWGGHIWDLKGMPYSQEE
                     LRCLYAFGPDPCHDTSFHPEDYTILSNYRHPLFPNDSIFRFEPHGYNASEFDTTCGSD
                     TLCTAVRKVYPEMPDPRTLPVDSITPEMFTAEGYFKFYEYEFTIEHLLPTVAYWVNVT
                     AYDVGSPGAGVYPLESDKTAGLKIVYPSSPYDAKG"
BASE COUNT          287 a          535 c          530 g          279 t
ORIGIN      
        1 gccccttcgc gtcataaggc gaagacgggt agacgatctt cagccccgcc gtcttgtcgg
       61 actccagcgg gtagaccccc gcgccgggac tcccgacatc gtacgccgtc acattcaccc
      121 agtacgccac ggtcggcagc aagtgttcga tcgtgaactc gtactcgtaa aacttgaaat
      181 acccctcggc cgtgaacatc tccggagtga tcgaatccac cggcaacgtc cgcggatcgg
      241 gcatctccgg atacactttc cgcacggcag tgcagagcgt gtcgctcccg caggtagtgt
      301 cgaactccga ggcgttgtag ccgtggggct caaaccggaa aatggaatcg ttggggaaga
      361 gcgggtggcg gtaattcgac aggattgtgt agtcctcggg gtggaaactc gtgtcgtgac
      421 aggggtcggg gccgaaggcg tagaggcagc ggagttcctc ctgcgagtac ggcatcccct
      481 tgaggtccca gatgtgtccc ccccagccat caggcttctt cacccacttg tcgtagttgc
      541 gccggtcgaa cgagccgacc acggtgagcg cttccgctcg ctcgtcgcgg ccgaggtaga
      601 tgcgatagcc ctcgaaatcc ggcgcctgca ggagcggatc gacctgggtc tcggacacct
      661 ggccgttgaa ccggacatgg acgccgcccg ggacgggggt gagccagaac ggggggggcg
      721 gaggcgggta ggcgacccgg aaatccggga tgccgtcccc cttataccac tgggtgtcgg
      781 ctttctgggc gacccactgg ccgcccacca gggcggaatc ctggacacag acgcggaatt
      841 cgccggcata cccgtcgcca tccgtgtcga aaccggggtt gtcgaacacg cgctgggccc
      901 agcgggcgtt cgcgatcagg tccgagaaat ccagccgttg ttcgtactcg gccggccgcc
      961 gcgggatgta ggcgaggatc cacggatcct tgtggatcag ttctccgccg accacggcaa
     1021 acgagagcgt caccttgtgc ccggccgcca gcgtcagcgg tccccacgag accaggtagt
     1081 cgggcgtcgt gttcgaggcg atcgcattcg cctcggtcag cggcaccgga ttccaggggg
     1141 gcgtcagggc gcgcgcaatc tcccccgtcc agtagttcgg gtggtcctgt tcgtgcgtgg
     1201 agagccgccg gtactccgac tcgagcgaat agaaccagtc ctgattgtac caccagcttc
     1261 cccggccgaa tgacctcacc gacggatcgg cggagggctc catgaagatc ggcccgagac
     1321 tgggcgccca ccggctcgtc caccagttgt agtagacgcc cccgcggtcc accggcccgc
     1381 ggagggggac tatccccaga ccgctgcgca ccgaggccag catgcacatg gtgtcggcga
     1441 tatccacctg tgttcccacc ggcctgtcat agaacacccc cccgtaggcg ttgccgtcgc
     1501 ggtcggcgtg ccaggctagg ttgagcgtgt cccggaaacc gcagccgatc ggcgcctcgt
     1561 acgtctcaaa aaaccccacc acctcgcccg agtcattgca cggccgcgtg ttgttgtagc
     1621 gcgcggtcgg g
//
LOCUS       DAOOKS010000509         1626 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_97676_length_1626_cov_3.330363, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000509 DAOOKS010000000
VERSION     DAOOKS010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97676_length_1626_cov_3.330363"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1042
                     /locus_tag="PK112_07965"
     CDS             <1..1042
                     /locus_tag="PK112_07965"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPC12019.1"
                     /translation="AMHYTLLRDSLAGTNGTVLLWFMHICSERGLYAQTTVVGAEILR
                     RYGASPLVAEARYLYAEALTRLGRYAEAVAVYDSIVAAYPRVQDKAEAACRIGQIYLN
                     YLNEPRRALRLFDSVAAAYPGGLGYLRALLAAPDAYLRLGELDSARTALGRLAQRPLI
                     EEAAEEAAFKLAKIDFYEKRFDTARAGFNKLLVAYPKGFYVNDALQMMLLIDEAGSEP
                     LLSEFSDALLFEERREYDSCRAELERIAGADHPALSDDALYRLADLSIARFDSAAALE
                     YIERLAGQYPESYFAPWGLKLKGDMLTADPRRREEGKAIYRRLLEENANYPFTSDVRK
                     RLRELEVDPRTG"
     gene            complement(1061..1309)
                     /locus_tag="PK112_07970"
     CDS             complement(1061..1309)
                     /locus_tag="PK112_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12020.1"
                     /translation="MFVWCSLLFVLGILAFLDSVFNMGMIFRQVNSVFFMLVSLALLV
                     RTTTKMKEKKVESYQDKVFQLERRVRTLEEGRKKLETY"
BASE COUNT          279 a          510 c          550 g          287 t
ORIGIN      
        1 ggcgatgcac tacacgctgc tgcgcgattc gctcgcgggg accaacggga cggtgctgct
       61 gtggttcatg cacatctgca gcgagcgggg tctgtacgcg cagacgaccg tggtgggggc
      121 ggaaatcctg cggcgctacg gcgccagccc gctggtggcc gaggcccgct acctctacgc
      181 cgaggcgctc acgaggctgg gacggtacgc cgaggcggtc gccgtgtacg attcgatcgt
      241 ggcggcctac ccgcgcgtcc aggacaaggc cgaggcggcc tgccggatcg ggcagatata
      301 tctcaactac ctcaacgagc cgcggcgggc gctgcggctg ttcgattccg tggcggccgc
      361 ctaccccggg gggctggggt atttgcgggc cctcctggcg gcgcccgacg cctaccttcg
      421 cctcggcgaa ctggacagcg cccggacggc actcgggcgg ctggcgcagc ggccgctcat
      481 agaagaagcg gccgaggaag cggccttcaa gctggccaag atcgatttct acgagaagcg
      541 gttcgacacg gcgcgggcgg gcttcaacaa actcctcgtg gcctacccca aggggttcta
      601 tgtcaacgac gcgctgcaaa tgatgctgct gatcgacgag gcggggtcgg aaccgctcct
      661 gtccgagttc tccgacgccc tcctcttcga ggagcggcgc gagtacgatt cctgccgggc
      721 ggagctggag cggattgccg gggcggacca cccggcgctc agcgacgacg cgctctaccg
      781 gctggccgat ttgagcatcg cccggttcga ttccgcggcc gcgctggagt atatcgagcg
      841 gctggccggg cagtatcccg agtcatactt cgccccgtgg ggactgaagc tcaaggggga
      901 catgctcacg gccgaccccc ggcggcggga ggagggcaag gcgatctacc gccggctgct
      961 cgaggagaac gcgaactacc cgttcacctc ggacgtgcgc aagcggctcc gcgagctcga
     1021 ggtcgatccg cggacgggct gacggcgcgc gttgccggcc tcagtaggtc tccagcttct
     1081 tgcgcccctc ttcgagcgtg cggacgcggc gctccaactg gaacaccttg tcctggtagc
     1141 tctccacctt cttctccttc atcttggtcg tcgtgcgcac caggagcgcg agcgacacga
     1201 gcatgaagaa gacggaattg acctggcgga agatcatgcc catgttgaac acggaatcca
     1261 gaaaggccag gattcccagc acgaagagca aactgcacca tacaaacata cgtgcctccg
     1321 aatgcggccc gggtcccgcg gccggtcccg ggcgtaaaat tagaggttgt gcccggcagg
     1381 gccgccgtta tatttacctt ttcaagggac ggtcccttgc ctctagtgta tcggtaagcc
     1441 gagaggactc gtttaggagg agacggtggc tgacttatct ctgacggacc cggacaagtt
     1501 cgaccggacg aacgccgtgg cggcgggggc ggtgtttctg gtgtcgttca ttacgtacgc
     1561 gctgacggtg cagccgacgt tttcattctg ggattgcggc gaattcattg caacgtcgta
     1621 cattct
//
LOCUS       DAOOKS010000510         1626 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_97723_length_1626_cov_1.820496, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000510 DAOOKS010000000
VERSION     DAOOKS010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97723_length_1626_cov_1.820496"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="PK112_07975"
     CDS             <1..528
                     /locus_tag="PK112_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12021.1"
                     /translation="GALTIKGLAETQVVPFTDPPSTAGAYTNDINCLVRGPKSSWSIQ
                     FTAVNSLGARATKTLKVLNPRKKMTISLTQIGKAVLIVGPSTYYECALRLEVHDPDNA
                     LIGGTMLATTIHTEEWTIEEGICTELGASHCRCEAGLFWLVAENVHIPRHKPLKVTII
                     NRNGKRVSASLTIRP"
     gene            632..>1626
                     /locus_tag="PK112_07980"
     CDS             632..>1626
                     /locus_tag="PK112_07980"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPC12022.1"
                     /translation="MASPIRRVHFIEFNAKVGTLQMYPVFPKYGTPLLAALLKGRGYD
                     ARIFLEGVSDMAFEKLADCDAACFPVFAPALNKVRECAARFRAARPKTPLIAGGPQAC
                     LFPETVAGLCDFVVRCEGDEALPELLDRLGRGEDAHALPGISYRRNGATVHNPDRPPP
                     AVPDLGPDFALIEGFDRLGRGPARMRRVVNTLQTSRGCRFKCKFCPTAKLFGGSYRTR
                     SIDAVVADIRARRRHNPFFFVVDNSFLGDRAHAVKLLDRLARERLGAAFIVFERHEIG
                     FDDEMLALLARAGVRCIIVGMESLEDSNLQAYDKRQTSAQAIRAVENIRRHGMHV"
BASE COUNT          272 a          594 c          500 g          260 t
ORIGIN      
        1 ggcgcgctga cgatcaaggg tctggccgag acgcaggtcg ttccgttcac cgaccccccg
       61 tctacggccg gcgcctacac caacgacatt aattgcctcg tgcgcggccc gaagagcagt
      121 tggtcgatcc agttcaccgc cgtcaactcc ctcggagcca gggccacgaa aacgctcaag
      181 gtgctcaatc cccgcaagaa aatgacgatc tccctgactc agatcgggaa ggccgtcctc
      241 atagtggggc cgtcgacata ctacgaatgc gcgctgcgcc tcgaggtcca cgaccccgat
      301 aacgcgctga tcggaggcac gatgctggcc acgacgatcc ataccgagga gtggaccatc
      361 gaagagggaa tctgcaccga gctcggtgcg tcgcactgcc ggtgcgaggc cggtctcttt
      421 tggctggtgg ctgagaatgt ccatataccg cgccacaagc cgctgaaggt gacgatcatc
      481 aaccggaacg gaaaacgcgt gtcggcgtcg ctcaccattc ggccgtagcc gccgcgccgc
      541 ggcttcgtgc cggcgtcggc ttgaaccgct aatcttgcgc cgccggccga gacccgggcc
      601 ggatggccgt ctccttcccg gaggaggaac catggcgtcc ccgatcagac gcgttcactt
      661 catcgagttc aacgcgaaag tcggcaccct ccagatgtac cccgtgttcc ccaagtacgg
      721 cacgccgctc ctcgcggcgc tcctgaaagg gcggggctac gatgcgcgca tctttctgga
      781 gggcgtcagc gacatggcct tcgagaagct ggccgactgc gacgccgcct gcttccccgt
      841 gttcgcgccg gcgctcaaca aggtccggga gtgcgcggcg cggttccgcg cggcgcggcc
      901 gaaaacgccc ctcatcgccg gcggcccgca ggcctgcctc ttccctgaga cggtggccgg
      961 cctctgcgat ttcgtcgtgc gatgcgaggg tgacgaggcg ctccccgaac tcctcgaccg
     1021 cctcggacga ggcgaggacg cccatgcgct ccccggcatc tcgtaccggc gcaacggcgc
     1081 caccgtccac aatcccgacc gcccgccccc cgcggtcccc gacctcggtc ccgacttcgc
     1141 gctgatcgag gggttcgacc ggctggggcg cggcccggcg cgcatgcgcc gcgtggtcaa
     1201 cacgctgcag acctcgcggg gctgccgctt caaatgcaag ttctgcccta cggccaagct
     1261 cttcgggggc tcctaccgca cgcgaagcat cgacgccgtc gtcgccgaca ttcgcgcccg
     1321 gcgccgccac aatcccttct tcttcgtcgt cgacaacagc ttcctgggcg accgcgccca
     1381 cgccgtgaag ctgctcgatc gcctggcccg ggagcggctc ggcgcggctt tcatcgtctt
     1441 cgaacgccac gagatcggct tcgacgacga gatgctggcc ctgctcgccc gagccggcgt
     1501 ccggtgcatc atcgtcggca tggagtccct cgaagactcc aacctgcagg catacgacaa
     1561 gcgtcagacg agcgcccagg ccatccgcgc cgtcgagaac atccggcgcc acggcatgca
     1621 cgtcat
//
LOCUS       DAOOKS010000511         1624 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_97883_length_1624_cov_2.244742, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000511 DAOOKS010000000
VERSION     DAOOKS010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97883_length_1624_cov_2.244742"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1273)
                     /locus_tag="PK112_07985"
     CDS             complement(<1..1273)
                     /locus_tag="PK112_07985"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HPC12023.1"
                     /translation="MITYSEVRRLNARGNVIPVFARIPADLVTPVAAYRKLALGKRES
                     FLLESVEGGEKVARYSFLGFDPFLVIEGDDGRVRLIRGRRRHTVEAGPLKFVRELFRF
                     YRPVPVAGLPRFTGGAVGYFAYDTARWIEKIPDANSDPIGLPVMHLGLYRHLVVFDHL
                     KQEILLIANILHDQGKRGLRAKYDEAAATVRSMEQRLSAPARSRPGRPAGRARVTPLC
                     TEAEYRRMVARAKRYIKEGDIFQVVLSRRWRVETEVDPLTVYRRLRRINPSPYMFLLA
                     FGDAAVVGASPEMLVRIEQGTIETRPIAGTRPRGSSEAEDARRIADLLADPKELAEHT
                     MLVDLGRNDLGRVSVPGSVSVPQHMLIERYSHVIHIVSSVVGRLRRGVSPVDGLAACF
                     PAGTVSGAPKVRAMEIIDELEKHRRGVYAGAV"
     gene            complement(1270..>1624)
                     /locus_tag="PK112_07990"
     CDS             complement(1270..>1624)
                     /locus_tag="PK112_07990"
                     /inference="COORDINATES: protein motif:HMM:NF013813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HPC12024.1"
                     /translation="PHTVLFVDDFDFDWRTLGADVERHRAFPRRTNVEFVKIVSRRKL
                     RVAEWERGAGATGSSGTGAAAAVAAAVISGFADRACEVVFDSGSLFVDWHEETDIIEL
                     TGPVTYVGGGVFEGR"
BASE COUNT          277 a          558 c          542 g          247 t
ORIGIN      
        1 cgaccgcccc ggcgtagacc ccgcggcggt gcttctcgag ttcgtcaatg atctccatgg
       61 cccgcacttt gggcgccccg gagacggtgc cggcgggaaa acaggcggcc agaccgtcga
      121 ccgggctcac tccgcgccgg agacgcccca ccaccgagct cacaatgtgg atcacgtggg
      181 agtagcgctc gatgagcatg tgctgtggga cactgaccga acccgggacg ctgacgcggc
      241 ccagatcgtt gcggccgaga tcgaccagca tggtatgctc ggccagttct ttcgggtcgg
      301 ccagcaggtc ggcgatccgg cgggcatcct cggcctcgct gctcccccgc ggccgggtgc
      361 cggcgatggg gcgggtctcg atggtgccct gctcgatccg cacgagcatc tccggggagg
      421 cgccgaccac agcggcatcg ccgaatgcca ggaggaacat gtagggggag ggattgatgc
      481 ggcgcaggcg gcggtacacg gtcagcggat cgacctcggt ctccacccgc cagcgccggg
      541 agaggacgac ctggaagatg tccccttcct ttatatagcg cttggcgcgg gcgaccatgc
      601 ggcggtactc ggcctcggtg cacagcggcg tcacccgggc ccgtccggcg gggcggccgg
      661 ggcggctccg ggccggcgca ctcagccgct gctccatcga gcggaccgtg gccgcagctt
      721 cgtcgtactt ggccctcagc cccctcttcc cctggtcgtg caggatgttg gcgatcaaga
      781 ggatctcctg cttgaggtgg tcgaaaacca cgaggtggcg gtacagaccg aggtgcatga
      841 ccggcagtcc gatcgggtca gagttggcgt cagggatctt ctcgatccag cgggctgtgt
      901 cgtaggcaaa gtaccccacc gcgccgcccg tgaagcgcgg caggcccgcc acgggaaccg
      961 gacgatagaa ccggaacagc tcgcgcacga atttcagcgg cccggcctcc acggtgtggc
     1021 ggcggcgacc ccggatcagc ctcacccgcc cgtcgtctcc ttcgattacg agaaacgggt
     1081 caaacccgag aaacgagtag cgcgcgactt tctctccccc ctcgaccgat tccagcagaa
     1141 acgactcccg cttgccgagc gccagtttcc ggtaggccgc taccggtgtc accaggtcgg
     1201 cggggatgcg tgcgaacacc gggatcacgt tcccgcgggc attgaggcgg cggacttcac
     1261 tgtaggtgat catcgccctt cgaaaactcc cccaccgaca taagtgacgg ggccggtgag
     1321 ctcgattatg tcggtctcct cgtgccagtc gacaaagagc gagccggagt cgaaaaccac
     1381 ctcgcacgcg cggtcggcga acccggaaat cacggccgcc gcgacggcgg ccgccgcgcc
     1441 ggtgccggaa gacccggtgg cgccggcgcc cctctcccac tccgcgaccc ggagcttcct
     1501 gcggctgaca atcttcacga actcgacatt ggtgcggcgc ggaaacgccc ggtgccgttc
     1561 cacgtcggcg ccgagcgtcc gccaatcgaa atcaaaatcg tccacgaaca gaaccgtgtg
     1621 cggg
//
LOCUS       DAOOKS010000512         1621 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_98120_length_1621_cov_3.366539, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000512 DAOOKS010000000
VERSION     DAOOKS010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98120_length_1621_cov_3.366539"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..872
                     /locus_tag="PK112_07995"
     CDS             <1..872
                     /locus_tag="PK112_07995"
                     /inference="COORDINATES: protein motif:HMM:NF015436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HPC12025.1"
                     /translation="LVFIRIAWFFSWILTALANLVLRILGIRRDSEGQAVTEDEINLM
                     IFEGKQSGVFDETEEQLVRNVFDFADSTVRRAMTPRTDVVGLPLDGDPAQVIAVVIDN
                     GYSRYPVYEGSLDRIVGILHTRDLVLHRLNAHEFALRDLIRKPVFVPDSLPLARLLRD
                     FQRKKNKFAVVLDEFGVTAGIVTLEDILEQLVGEILDEDERGELPLVRHSESVAYADG
                     AVRPGAVNVLMDARLPEDRAETLAGLVIDHLGHLPSKDDSVIIGDMKITVLELADNRL
                     QRLKLERLAVPTA"
     gene            891..1559
                     /locus_tag="PK112_08000"
     CDS             891..1559
                     /locus_tag="PK112_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018046265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HPC12026.1"
                     /translation="MTGIVTAPLRWMRALYDWVIRWSESRHARTALFFLSLAEASFFP
                     IPPDALLIALCMGAYRRWAKFALICSLGSVFGGMLGYLIGHSAFELIGSHILSWTASL
                     SGSDPEALLQTARYWFNEKELWGMKVGAWAVGIAGFTPIPYKVFTIAAGFFEMSFPVF
                     VIASVLSRSARFFLVAGLIGLLYRRHGRRIQVFIDKYFNALAIAFVLLLAGGFWAISL
                     MKGD"
BASE COUNT          233 a          568 c          477 g          343 t
ORIGIN      
        1 tcctcgtctt catccggatc gcctggtttt tctcctggat cctcaccgcc ctggccaatc
       61 tggttctccg cattctcggc atccgccgcg attcggaggg ccaggcggtc accgaggacg
      121 agatcaacct gatgatcttc gagggcaagc agtccggcgt gtttgacgaa accgaggagc
      181 agctcgtccg caacgtgttc gatttcgccg actccaccgt ccggcgcgcc atgactcccc
      241 gcaccgacgt cgtcggcctt ccccttgatg gcgacccggc gcaggttatc gccgtcgtca
      301 tcgacaacgg ctactcgcgg tacccggtgt acgaggggag cctcgaccgc attgtgggga
      361 tcctccacac ccgcgacctc gtgctccacc gcctgaacgc gcacgagttc gcgctccggg
      421 atctcatccg caaacccgtt tttgtccccg actccctgcc gctcgcgcgg ctcctgcgcg
      481 atttccagcg caagaagaac aagttcgcgg tggtgctcga tgaattcggc gtgaccgccg
      541 gcatcgtcac gctcgaggac atcctcgagc agttggtggg ggagattctc gatgaggacg
      601 agcgcggcga gctcccgctc gtccgccact ccgagtcggt cgcgtatgcc gacggggcgg
      661 tgcggccggg ggcggtgaac gtgcttatgg acgcccgcct tcccgaggac cgcgccgaga
      721 ctctcgccgg gctcgtcatc gaccaccttg ggcacttgcc ttccaaggac gacagcgtta
      781 ttattggcga catgaagatc accgtgctcg agctggccga caaccgcctc cagcggctca
      841 aactggagcg gctagccgtc ccgacggcct gaccgggcgg gcgaatcggt atgacaggca
      901 tcgtcacggc tcccctccgc tggatgcgcg cgctctacga ctgggtgatc cggtggagcg
      961 agtcccgcca tgcccggacg gcgctgtttt tcctctcgct ggccgaggcg tcgttcttcc
     1021 cgatcccgcc cgacgctctc ctcatcgcct tgtgcatggg cgcgtatcgg cgctgggcga
     1081 agttcgcgct gatctgttcg ctcggttccg tcttcggggg catgctcggc tacctcatcg
     1141 gccactccgc tttcgaactg atcgggtcac acattctgag ctggacggca tcgctgtccg
     1201 gttccgaccc cgaggccctc ctgcagactg cccgctattg gttcaatgag aaggagctct
     1261 gggggatgaa agtcggcgcc tgggcggtcg ggatcgccgg tttcacgccc attccctaca
     1321 aagtcttcac catcgccgcc ggcttcttcg aaatgtcctt tcccgtcttc gtgatcgcct
     1381 ccgtcctcag ccgctcggcc cgctttttcc tcgtcgccgg cctcatcggt ctgctctacc
     1441 gccgccacgg ccggcggatc caggttttta tcgacaagta cttcaacgcc ctcgccatcg
     1501 ctttcgtgct ccttctcgcg ggcggattct gggctatcag cctcatgaag ggcgactgat
     1561 cccgctctcc ccgccggctc ccggcaacgg agtcttgctt tgtcggcccg atgagttata
     1621 c
//
LOCUS       DAOOKS010000513         1621 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_98146_length_1621_cov_2.551724, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000513 DAOOKS010000000
VERSION     DAOOKS010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98146_length_1621_cov_2.551724"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(45..1289)
                     /gene="icd"
                     /locus_tag="PK112_08005"
     CDS             complement(45..1289)
                     /gene="icd"
                     /locus_tag="PK112_08005"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase (NADP(+))"
                     /protein_id="HPC12027.1"
                     /translation="MAKYKHIVVPSEGTRIVVKNKQLQIPDEPIIPFIEGDGTGRDIW
                     KATRRVVDAAVEKAYGGRKRIVWMEIYAGEKANELYKEWLPDETVDAIRKYVVALKGP
                     LTTPIGGGFRSLNVSLRQFLNLYACVRPVRYFRGVGSPVCHPEKLNVVIFRENTEDVY
                     AGIEWQKGSKEAQKVIAWLNKNMKQAIRTDSGIGIKPISMTGTTRLVRKAIKYALEKK
                     HKSVTLVHKGNIMKYTEGAFRDWGYRVAKKEFGAHTITEDELWSKYDGKQPEGKLLIK
                     DRIADSMFQQILTRPDEYEILATPNLNGDYLSDACAAQVGGLGMAPGANIGDFVALFE
                     ATHGTAPKYADKDMINPGSLILSAAMMLDYLNWPEAGALIHSALQTTIDRKTVTYDLE
                     RQMPGATKVGTAEFGMQIIKNM"
     gene            complement(1301..>1621)
                     /locus_tag="PK112_08010"
     CDS             complement(1301..>1621)
                     /locus_tag="PK112_08010"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714988.1"
                     /note="Catalyzes the reversible oxidation of malate to
                     oxaloacetate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HPC12028.1"
                     /translation="KAIEERTANGGGEIVKLLKTGSAYYAPAAATVQMVRAILNDEKR
                     LLPCSCYLTGEYGIEDMHIGVPCILGAKGVEKILELKLNKAELTSLQKSAATYKEHMK
                     IMGY"
BASE COUNT          237 a          561 c          454 g          369 t
ORIGIN      
        1 aggacgctcg cggtcctgcc ttttcatgtg tcggccggtc cgatctacat gttcttgatg
       61 atctgcattc cgaactccgc ggtcccgacc ttggtcgccc ccggcatctg gcgttccagg
      121 tcgtaggtga ccgtcttccg gtcgatcgtc gtctgcagcg ccgagtgaat caatgcaccg
      181 gcctcgggcc agttcaggta atccagcatc atcgccgccg acaggatcag cgagccgggg
      241 ttgatcatgt ccttgtcggc gtacttgggc gccgtgccgt gggtcgcctc gaacagggcc
      301 acgaaatcgc cgatgttcgc gcccggcgcc atccccaggc ccccgacctg cgcggcgcac
      361 gcatccgaca ggtagtcccc gttcagattc ggcgtcgcca ggatctcgta ctcgtccggc
      421 cgggtgagaa tctgctggaa catcgaatcg gcgatccggt ccttgatcag cagcttgccc
      481 tccggctgct tcccgtcgta cttcgaccac agctcgtcct cggtgatcgt gtgggcgccg
      541 aactccttct tcgccacccg gtatccccag tcgcggaacg cgccctcggt gtacttcatg
      601 atgttcccct tgtgcaccaa agtcaccgat ttgtgcttct tctccagcgc gtacttgatc
      661 gctttgcgca ccagccgggt ggtcccggtc atcgagatcg gcttgattcc gatcccgctg
      721 tcggtccgga tcgcctgctt catgttcttg ttcagccagg cgatcacttt ctgcgcctcc
      781 ttcgacccct tctgccactc gatcccggcg tagacgtctt ccgtgttttc gcggaagatg
      841 acgacgttga gcttctccgg gtggcagacg ggtgacccga cgccgcggaa atagcgcacc
      901 ggccggacgc aggcgtagag gttgaggaac tggcgcaggc tcacgttgag cgagcggaac
      961 ccgcccccga tcggcgtggt gagcggcccc ttgagcgcca ccacgtattt gcggatcgca
     1021 tccacggtct cgtccggcag ccactccttg tacagctcgt tggccttctc ccccgcgtag
     1081 atctccatcc acacgatccg cttcctgccg ccgtaggcct tctcaaccgc cgcatcgacg
     1141 acccgccgcg tcgctttcca gatatcccgc ccggtgccgt ctccctcgat aaacgggatg
     1201 atcggctcat cggggatctg gagctgtttg ttcttgacga cgatgcgcgt cccctcgctc
     1261 gggacgacga tatgcttgta cttcgccatc gctcgctcct ctagtatccc atgatcttca
     1321 tgtgttcttt gtaagtggcc gccgacttct gcagcgacgt cagttccgcc ttgttcaact
     1381 tcagctcgag gatcttctcc acacccttgg cgccgagaat gcacggcacg ccgatgtgca
     1441 tgtcctcgat cccgtactcg cccgtgaggt agcacgagca cggcaggaga cgcttctcat
     1501 cgttgaggat cgcccgcacc atctgcacgg tcgctgccgc cggtgcgtag taggccgacc
     1561 cggttttgag cagcttgacg atctcgccgc ccccgttggc cgtccgttcc tcgatcgcct
     1621 t
//
LOCUS       DAOOKS010000514         1620 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_98220_length_1620_cov_2.797444, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000514 DAOOKS010000000
VERSION     DAOOKS010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98220_length_1620_cov_2.797444"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1582)
                     /locus_tag="PK112_08015"
     CDS             complement(<1..1582)
                     /locus_tag="PK112_08015"
                     /inference="COORDINATES: protein motif:HMM:NF012999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPC12029.1"
                     /translation="MNDVAVFSMRLARLLAALAVVAILTGGAAAASGSANPTGQPKPA
                     GSQDVLPPAFSEGFTLPAPEAVALEPEIVPSLGDPKVAPSVNVMPGKFVGRGEPYTWV
                     GRILTIWGNVKNGAPPLTFEWQFGDGSAPVTGSVSNAKYIATTHTYASSGIKRAVLTV
                     TDADGFSDRDTVIIDVIGTPTLKDSINASVEDGLRYLYNSQNTGGLWIGYSSYYVSAT
                     AQAVLCFENQGYLPSDDPNENIYVECIQQGLDYLTANLRPVAIGLQTYCDPEAVAPGN
                     TDANGLGIYPVSGSPYEAGPIMMALAGSGDAMKTHPAGTGVANVIGRSYYEILVDMVD
                     WCAWAQNESGRGGWRYSANYSSSDNSVSQWPTIGLEAAEKQWGISPPPCVKSELRLWI
                     NASQLANGRFTYYPSTYATSYVPMTGAGLCEMSFVGIPTDSGRVKRAMQQLFLGWSDY
                     YGKGFPYAMYGIKKGCDIARPEPIEDLDTTAGYFDWYSDYARYLLAPGRQNATTGQFL
                     IHHGYLGDARIHDHTWCIL"
BASE COUNT          278 a          479 c          569 g          294 t
ORIGIN      
        1 caaggatgca ccaagtgtgg tcatggatgc gggcgtcgcc caggtagccg tgatggatga
       61 ggaattggcc ggtggtcgca ttctggcggc cgggagccag caggtagcgg gcgtagtcgg
      121 aataccagtc gaagtagccg gcggtggtgt cgagatcctc gatcggttcg gggcgggcga
      181 tgtcgcagcc cttcttgatg ccgtacatgg cgtacgggaa ccccttgccg tagtagtcgg
      241 accagccgag gaagagctgc tgcatggccc gcttgacccg gccgctgtcg gtgggaatgc
      301 cgacaaagga catttcacac aggccggctc cggtcatcgg cacgtagctg gtggcgtacg
      361 tgctcgggta gtaggtgaag cgtccgttgg cgagctggct ggcgttgatc cagagccgca
      421 gttccgattt cacgcagggc ggcggcgaaa tgccccactg cttctcggcc gcctccaggc
      481 cgatcgtcgg ccactgggag acggagttgt ccgaggagct gtagttggcc gaatagcgcc
      541 agccgccgcg gccggactcg ttctgggccc aggcgcacca gtcgaccatg tcgacgagga
      601 tctcgtagta ggagcggccg atgacgttgg cgacgccggt gccggcggga tgcgtcttca
      661 tcgcgtcgcc cgaaccggct agcgccatca tgatcggccc ggcctcatac ggggatcccg
      721 agacggggta gatgccgaga ccgttggcgt cggtgttgcc gggagcaacg gcttccggat
      781 cgcagtacgt ctgcaggccg atcgcgaccg gtcgcaggtt ggcggtgagg taatcgagcc
      841 cctgctggat gcactcgacg tagatgttct cgttcggatc gtcgctcgga aggtagccct
      901 gattttcaaa acagagaacc gcctgcgcgg tcgcgctgac atagtaactc gagtacccga
      961 tccagagacc cccggtgttc tgcgaattgt agaggtaccg cagtccgtcc tccaccgagg
     1021 cgttgatgct gtccttgagc gtcggcgtgc cgatgacgtc gatgatgacg gtatcccggt
     1081 cggagaatcc gtcggcatcg gtgacggtca ggacggcgcg cttaatgccg gaggacgcat
     1141 aggtgtgggt cgtggcgata tatttggcgt tgctcaccga tcctgtgacc ggggcgctgc
     1201 cgtcgccaaa ctgccactcg aaggtcaggg ggggcgcgcc gttcttgacg ttgccccaga
     1261 tggtcaggat gcgcccgacc caggtgtacg gttcgccgcg gcccacgaat ttgccgggca
     1321 tgacattgac gctgggagcc accttggggt cgcccagcga cggaacaatc tcgggctcca
     1381 aggccaccgc ctcgggggcg ggaagagtga aaccttcgct gaacgcgggc gggagcacgt
     1441 cctgactgcc cgccggtttc ggctgccccg tcgggttcgc gctgccggag gctgccgcgg
     1501 cgccgccggt gagaatcgcg acgacggcga gcgccgcgag gagacgcgcc agccgcatcg
     1561 aaaagaccgc tacatcgttc atgtgaatcc accttgctcg aaacgagcat ctgtccaaat
//
LOCUS       DAOOKS010000515         1618 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_98412_length_1618_cov_3.206654, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000515 DAOOKS010000000
VERSION     DAOOKS010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98412_length_1618_cov_3.206654"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="PK112_08020"
     CDS             complement(<1..620)
                     /locus_tag="PK112_08020"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HPC12030.1"
                     /translation="MKQLILMAGALLMLGIAASAQESPAGVTGKGVKLGLDFSRINTE
                     YDELDDFLDSRVGFTAGAFLTYRVSRQFAVQPEILYVAKGAEKDFFFYTPEWATEYLE
                     IPVLLKFDLMPAGPAHPNLFAGPALDFLLGSEFRVSDYRIDLSDYTQSVDVGLVFGGG
                     IDYGYFTFDARYTVGLIGMVDGFDDFAAAVNEATGAEPGDSYYLDG"
     gene            1233..>1618
                     /locus_tag="PK112_08025"
     CDS             1233..>1618
                     /locus_tag="PK112_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12031.1"
                     /translation="MNRRYVETKVLAQHGPSTHLLIKAGDTVVQANYAEVAPCAFPVW
                     TGAPVYVEIPAHGVKNSVPGSFSRAPEPQDASGELPNIIKFPVYTHQEETALQEFEDL
                     AGPSGRSNGATGPGSRHQPSTGVTTKP"
BASE COUNT          322 a          544 c          475 g          277 t
ORIGIN      
        1 tcgccgtcga gatagtaact gtcgcccggt tccgctcccg tcgcctcgtt gaccgcggcg
       61 gcgaaatcat cgaagccatc gaccatgccg atcaatccca ccgtgtaccg cgcgtcgaaa
      121 gtgaagtacc cgtagtcgat gccgccgccg aagacgagcc ccacgtccac cgactgggtg
      181 taatcggaga ggtcgattcg atagtcgctg acccggaact ccgaaccaag gaggaaatcc
      241 agcgccggcc cggcgaagag gtttggatga gccggccccg caggcatgag gtcgaacttc
      301 agcagcaccg ggatttccag gtattccgta gcccattcgg gcgtgtagaa gaagaaatcc
      361 ttctccgctc ccttggcgac gtacaggatc tccggctgca ccgcgaattg gcggctgaca
      421 cggtaggtca gaaacgcacc ggccgtgaaa cccacccggc tgtccagaaa atcatccagt
      481 tcatcgtact cggtgttgat tcgggaaaag tcgagaccga gtttgacacc cttgccggtc
      541 accccggcag gcgactcctg cgcgctggcg gcgatgccca gcatcaatag tgctcccgcc
      601 atcaggatta gctgcttcat aggacctcct catcacgccg gtcgttgata acgccgtcat
      661 tttgtggcgg caatatggcg ggggctgatc gccccgccaa gataaaagcg gcctcccgcc
      721 atgatgatcg cggtgaaaaa acccttacgg atagcgcccg aaatcgccgc accacggccg
      781 ccgccgcgat atccgccgcc gagcgcggac ctcggtcaca acgatcgtct cggctgacgt
      841 atgatgacgg ggacggctgc gatttccggc ccgccggctc aggaattccc tgagctccac
      901 cgctcctttt ggcgaagcag gcgttcgccc gcgcgatgga aatcgtggcc ggaatcgttg
      961 cgtcggctca ggattctgtt cccgcgcggc tcgcctttat gaccgctccg actccgattt
     1021 ggcaaccccc acattccggc ggcgcccgat tgtcgcaatt tcttgtctgc cgttatttct
     1081 caacagagaa aacaaacggg tcgaaagggc cgacgctgac ggcagcgcgc cggccggttg
     1141 ggcggacaag cggcgccgaa tcggcttcac ttcgacccgc ccgttgcgaa gagaccagcc
     1201 gctgactcat aacccaatgg aggtaggtcg ccatgaaccg cagatacgtc gagacgaagg
     1261 ttctcgccca gcatggcccg tccacccacc tgctgatcaa agccggcgac acggtcgtgc
     1321 aggcgaacta cgccgaagtg gcgccgtgcg cctttcccgt gtggaccgga gcccccgtct
     1381 atgtcgagat tcccgcgcat ggagtgaaga acagcgttcc cgggtcgttc tcccgggccc
     1441 cagagcccca agacgcctcc ggcgaactgc ccaacatcat caagttcccg gtgtacacgc
     1501 accaggagga gaccgcgctt caggagtttg aagacctcgc cggtccgtcg gggcgcagca
     1561 acggcgccac cggcccgggc tcgcgccacc agcccagcac cggcgtgacc acgaagcc
//
LOCUS       DAOOKS010000516         1617 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_98533_length_1617_cov_2.502561, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000516 DAOOKS010000000
VERSION     DAOOKS010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98533_length_1617_cov_2.502561"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="PK112_08030"
     CDS             complement(<1..239)
                     /locus_tag="PK112_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12032.1"
                     /translation="MKKMAWGIALALILILALAGCGKEKAADKVDEAVQEAVINNALD
                     GEAEVDIDGDKYTIEDSEGNKVTVGGTEWPEGAAA"
     gene            complement(236..439)
                     /locus_tag="PK112_08035"
     CDS             complement(236..439)
                     /locus_tag="PK112_08035"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPC12033.1"
                     /translation="MTALTQREREVFRLLVEGYTLRETAQQLGLKYSTVNTHMTGIYR
                     KLGVNSRAELIIRYRDYDKEDAT"
     gene            complement(698..>1617)
                     /locus_tag="PK112_08040"
     CDS             complement(698..>1617)
                     /locus_tag="PK112_08040"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPC12034.1"
                     /translation="LRGISFSGFILLCCLLGMLFCSVSFFRERALIPEVGNQPFPKPA
                     WSIMAMVFLILAFNDVIVPLLLLQMKIAQNKPLEIWYFGGIVLGLLLVLLLQKYLQLN
                     ICLMLNLSLAFMAVGFVLSMPAETSDVLPGICAADFGLAYAVGMVNIYYLAGFMSKKF
                     RNITFYRAGIVLSAVYYFLAFFTFRLFEKALPVMVVGVVCILIAFFALSPVFVKLLYS
                     GEWIDDSYRPDVTFESRLRARLRELNLSPKETEVCELLLRGYILRQTAAMLEIAYSTA
                     NTYCTSLYRKLGINSRTELTLLFKEYIEA"
BASE COUNT          360 a          455 c          466 g          336 t
ORIGIN      
        1 tccgccgcgg cgccctcagg ccattcggtt ccgcccacgg tcactttgtt tccttcgcta
       61 tcttcgatgg tatatttgtc gccgtcgata tccacttcgg cttcgccgtc gagggcgttg
      121 ttgatcacgg cctcctgcac ggcctcatcg accttatcgg cggccttttc tttgccgcag
      181 cccgccagag ccagaatcag aatcagcgca agcgcaattc cccatgccat ttttttcatg
      241 ttgcatcctc cttgtcataa tcgcggtacc ggataatcag ttccgcgcgg gagttgactc
      301 ccagctttct gtatatgccc gtcatgtggg tgttgaccgt ggaatatttg aggccgagct
      361 gctgcgcggt ctcccgaagg gtgtagccct ccaccagcag gcgaaaaacc tcccgttccc
      421 gctgcgtcag cgcagtcatc ctctcctcct cgggacggac gccctccgga gaggcccgct
      481 ccgacgcggg tacaggttcc cgctttttga caaacgggct tctcagcttt tcccacaggg
      541 acattccgcc ggtcacctcc tccaagacgt ggcatgctgc caagcaactt tattttgagg
      601 cccggcaggg gattcggaca attcattagt tcatccgaaa agaaaaaagg tgcctcatta
      661 gttctaatga ggcacctttt tatagggtga cggcccttca ggcttcgata tattctttaa
      721 acagtagcgt cagttcggtg cggctgttga tgccgagttt gcggtagagg gaggtgcagt
      781 aggtattggc tgtggagtag gcgatctcaa gcatggcggc ggtctggcgc aggatatagc
      841 cgcgcagcag cagttcgcag acctccgtct ccttgggcga gagattcagt tccctcaggc
      901 gcgcccgcag gcggctttca aacgtcacat ccgggcggta gctgtcgtcg atccattccc
      961 cggaatagag cagtttcaca aagaccggag agagcgcaaa aaaggcgatc aggatacaca
     1021 ccaccccgac gaccatgacc ggcagggcct tttcaaagag gcggaaggtg aagaatgcga
     1081 gaaaataata gacggcggag aggacgatgc cggcccggta aaaagtgatg ttccggaatt
     1141 tcttgctcat gaatcccgcc agataataga tgttgaccat cccgaccgcg taggcgaggc
     1201 cgaagtccgc cgcgcagatg ccgggcagta cgtcggaagt ctccgcgggc atggagagca
     1261 cgaatccgac ggccatgaag gcaagcgaga gattcagcat caggcagatg ttgagctgca
     1321 ggtatttttg caagagcaaa acgagcagca gccccaagac gatgccgccg aaataccaga
     1381 tctccagggg tttattctgg gcgattttca tctgaagcag caggagcggc acgatcacat
     1441 cgttaaacgc cagaatcaga aacaccatgg ccatgatcga ccacgccggt ttggggaagg
     1501 gttgattgcc cacctccggg atcagggcgc gttcccggaa gaaggatacg gagcagaaca
     1561 gcatcccgag caggcagcag agcaggatga agccggagaa ggagattccc cggaggc
//
LOCUS       DAOOKS010000517         1615 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_98701_length_1615_cov_3.400000, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000517 DAOOKS010000000
VERSION     DAOOKS010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98701_length_1615_cov_3.400000"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            102..989
                     /locus_tag="PK112_08045"
     CDS             102..989
                     /locus_tag="PK112_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003330385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HPC12035.1"
                     /translation="MSLVTVLFIADVCGKVGRQALAHMVKPLRRKYGAHYVIANSENA
                     AGGFGITAEMARKVFAYGVNVQTSGNHIWDRYDIIRYLNENPRMLRPANFPEGVPGRG
                     CSVDRVDGVTIAVINLMGRTYMKDIDCPFQVARRLVEEVRRETSVIIVDMHAEATSEK
                     QAMLYWLDGKVSAVIGTHTHVATADEQVTPKGTAYITDVGMTGPYDSIIGMDKEPSLE
                     RFLTGMPKRFNTAEGDCRLAGAVLTIESETGRAHRIERIMYPFDIATFRPTDIAPADG
                     TEDAAPEEEFDNEADGEEV"
     gene            989..>1615
                     /locus_tag="PK112_08050"
     CDS             989..>1615
                     /locus_tag="PK112_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HPC12036.1"
                     /translation="MSAKIIDGKEVAASVLDRLQPRIAALRERGVTPGLAAVLVGEDP
                     ASATYVRSKAKKCEEIGLYSEVIRRPADIGQDELMRIVADLNANPKIHGVLVQSPLPR
                     HIDEMAVTLAIRPGKDVDGFHPYNVGMMALGFPRLLPCTPHGIVKLLEFYGLDPAGKE
                     VVVVGRSNIVGKPVAAMLLQKAAWANATVTVAHSRTANLAAVARRNPRA"
BASE COUNT          305 a          516 c          549 g          245 t
ORIGIN      
        1 gttgttgtaa actccgcctt tagtctgtgc ggccggcagg gtggaagcgg tgggcgcccc
       61 ggccgcggga aaccagtcgg cccgccctgg aaagttcact catgtcgtta gtcaccgtgt
      121 tgtttatcgc cgatgtctgc ggcaaagtcg gccgccaggc gctggcgcac atggtcaaac
      181 cgctgcggcg gaagtacggc gcgcactacg tcatcgccaa ctccgaaaac gccgccggcg
      241 gcttcgggat cacggccgag atggcgcgca aggtgttcgc ctacggcgtg aacgtgcaga
      301 cctcgggaaa ccacatctgg gaccggtacg acatcatccg gtacctcaac gagaaccccc
      361 gcatgctccg gccggcgaat ttccccgagg gcgtccccgg ccgcgggtgc tcggtcgacc
      421 gggtggacgg agtcaccatc gccgtgatca acctgatggg acggacctac atgaaggata
      481 tcgactgccc cttccaggtg gcccggcggc tggtcgagga ggtgcggcgg gaaaccagcg
      541 tgatcatcgt cgacatgcac gccgaagcca cctccgaaaa gcaggccatg ctctactggc
      601 tcgacggcaa ggtgtcggcg gtgatcggca cgcacaccca cgtggccacg gccgacgagc
      661 aggtgacgcc gaaagggacg gcctacatca ccgatgtggg gatgacgggg ccctacgatt
      721 cgatcatcgg catggacaaa gagccgtcgc tcgagcgctt tctcacgggg atgccgaagc
      781 gcttcaacac ggccgaggga gactgccggc tggccggcgc cgtcctcacg atcgaatcgg
      841 agacggggcg ggctcaccgg atcgagcgga tcatgtaccc cttcgacatc gccaccttcc
      901 ggcccaccga catcgcgccg gccgacggga cggaggacgc ggcgccggag gaggaattcg
      961 acaatgaagc cgacggggaa gaggtctgat gagcgcgaag attatcgacg gaaaagaagt
     1021 cgcagccagc gtgctggacc gcctccagcc gcgcatcgcg gccctccggg agcggggggt
     1081 gacgcccgga ctggcggccg tcctcgtggg cgaggatccg gcctcggcga cctacgtgcg
     1141 gtcgaaagcc aagaagtgcg aggagatcgg cctctactcc gaggtgattc ggcggccggc
     1201 ggatatcgga caggacgagc tgatgcgcat cgtggccgat ctcaacgcga atccgaaaat
     1261 ccacggcgtg ctcgtgcagt cgcccctgcc ccggcacatc gatgagatgg cggtcaccct
     1321 ggcgattaga ccgggcaaag acgtcgacgg ctttcacccc tacaacgtcg ggatgatggc
     1381 cctcggattt ccccggctgc taccctgcac gccgcacggc atcgtcaagc tgctcgaatt
     1441 ttacggcctc gatccggcgg ggaaagaagt cgtggttgtc gggcggtcga atattgtcgg
     1501 caaaccggtg gccgcgatgc tgctgcagaa ggcggcctgg gcgaatgcca cggtgacggt
     1561 ggcgcactcg cggaccgcaa atctcgccgc cgtggcccgg cgcaacccgc gcgcc
//
LOCUS       DAOOKS010000518         1610 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_99219_length_1610_cov_2.034084, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000518 DAOOKS010000000
VERSION     DAOOKS010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99219_length_1610_cov_2.034084"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..172)
                     /locus_tag="PK112_08055"
     CDS             complement(<1..172)
                     /locus_tag="PK112_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPC12037.1"
                     /translation="MKVRKLLHTRMRVNDIERSVKFYQDALGLEASRRFTSPRGAQLV
                     FLRTPNSEEEIEL"
     gene            complement(293..377)
                     /locus_tag="PK112_08060"
     tRNA            complement(293..377)
                     /locus_tag="PK112_08060"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(341..343),aa:Leu,seq:tag)
     gene            complement(509..1414)
                     /gene="asd"
                     /locus_tag="PK112_08065"
     CDS             complement(509..1414)
                     /gene="asd"
                     /locus_tag="PK112_08065"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374796.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HPC12038.1"
                     /translation="MSQDTVEFFHRKRRAMVEEKIPGEFWLRWLYSSSLSGRLALHGL
                     VKRAFFSWLYGRKMDKPRSVHRVLPFIVEHDLDVDEFAKQAMAYKTFNEFFARKLKAS
                     ARPIDPDPASAILPADGRHLVFPDVDAADGFYVKGDRFELGELIGGELVAKQFIGGAM
                     LISRLAPVDYHRFHFPCAGTPGRASEIKGPLFSVNPIALRRSVRYLVQNKRMVTLLET
                     ERFGLVALIEVGATMVGGIRQSFIPGTKVKKGDEKGLFKFGGSCVITLFQRDRIVFDA
                     DLREQSLNHIETYAKMGERFGVATR"
BASE COUNT          281 a          555 c          523 g          251 t
ORIGIN      
        1 agagttcgat ctcctcctcg ctgttcggcg tgcgcaggaa caccagttgc gccccgcgcg
       61 gcgaagtgaa gcggcgcgag gcctcgaggc cgagcgcgtc ctggtagaat ttcacggaac
      121 gctcgatgtc gttcacgcgc atccgggtgt ggagcagttt gcgtactttc atgacaactg
      181 atggcaggag atgggactca gcggcgtttt caagccccgg ccggatattg tatcctcccg
      241 cgcatcgccg gcggcgtgtc atccgaagct cgcgcggagc acgagcgaga gttggtgccc
      301 gggacagggg tcgaacctgc acaccttgcg gtacgggatc ctaagtcccg cgcgtctgcc
      361 aattccgcca cccgggcaat tcggctgggg atcgagcgcg gattcccggc gcccgccgcc
      421 ccgggcgcaa ggccgaaagg cgcgccgccc gacaggacac gaacgaccgc ggacaacagc
      481 cgcatcgccg gcgcgccgcg cgcgcgggct accgcgtggc cacgccgaac cgctcgccca
      541 tcttggcgta ggtctcgatg tggttgaggc tctgctcgcg caggtcggcg tcgaacacga
      601 tccggtcgcg ctggaacagc gtgatgacgc aggagccgcc gaatttgaag agccccttct
      661 cgtcgccctt cttcaccttc gtgccgggga tgaagctctg gcggataccg ccgaccatcg
      721 tcgcgcccac ctcgatcagc gcgaccaacc cgaaccgctc cgtctcgagg agcgtgacca
      781 tgcgcttgtt ctggacgagg tagcggacgc tacgccgcag cgcgatcgga ttgaccgaga
      841 agagcgggcc cttgatctcg ctggcgcggc cgggcgtgcc ggcgcagggg aagtggaacc
      901 ggtggtagtc caccggcgcg agtcgcgaga tgagcatcgc gccgccgatg aattgtttcg
      961 ccaccaactc gccgccgatc agttcgccga gttcaaagcg gtcgcccttc acataaaaac
     1021 catcggccgc gtcgacgtcg gggaacacga ggtggcgccc gtcggcgggc aggatcgcgc
     1081 tggcgggatc cgggtcgatc ggacgggcgg acgccttgag tttgcgggcg aagaactcgt
     1141 tgaacgtctt gtaggccatc gcctgtttgg cgaactcgtc cacatcgagg tcgtgctcca
     1201 cgatgaaggg gagcacccgg tggacgctcc ggggcttgtc catcttcctc ccatacagcc
     1261 acgagaagaa cgcacgcttc accagcccat gcagggcgag ccggccgctg agcgagctgc
     1321 tgtagagcca gcgcagccag aactcgcccg ggatcttctc ctcgaccatc gcgcggcgtt
     1381 tccggtggaa aaactcgaca gtatcttgcg acatgcccgc accgtgagca cgggcccgat
     1441 ccgcgccaaa ccgaaaacct ccggtccgcc gcaccccgtc gccgcaccgg tccggccacc
     1501 acggtatccg cccacccggc accacgtccc gaatccggga aacgaccgtc ccaccggcgg
     1561 gacatccctc cccgtgtccc gggaaaagcc cccgggacgt cgctgcgagc
//
LOCUS       DAOOKS010000519         1605 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_99668_length_1605_cov_2.296129, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000519 DAOOKS010000000
VERSION     DAOOKS010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99668_length_1605_cov_2.296129"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..1210)
                     /locus_tag="PK112_08070"
     CDS             complement(95..1210)
                     /locus_tag="PK112_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008496478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HPC12039.1"
                     /translation="MTREDRYRGNEDIVDRTEQLLKEITEANGVSGYEEEVRRLMARE
                     LRETADRIEYDRLGSIMGWKKGTADRPRVMVAGHMDEVGFMVREITSEGYIKFLPLGG
                     WWGHVALGQRMRVITRKGPVIGVVGSKPPHMLEAEERKKVLELKDMCIDVGVQEGFNA
                     KEALGIQLGDPIIPDSDFTIMGNPRMYLSKAFDNRMACAVVIDVFRALKGEGHPNTLL
                     GAGSTQEEVGLRGAQTLAHLGDPDAALIVDVGIGQDIPPNGFKKAEKLGGGPGILLFD
                     ASMIPNRRLRDLVTDTAEELKIPYHLSYMERGGTDAGRIHMSRTGVPSVVVGPPVRYI
                     HSHNGILNRDDYDNTVRLIAAVVRKLDARTVAGLTEV"
     gene            complement(1271..>1605)
                     /locus_tag="PK112_08075"
     CDS             complement(1271..>1605)
                     /locus_tag="PK112_08075"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPC12040.1"
                     /translation="SNTVFPEDVHLRELRRFGLSRFFDFTIFSSTFGKRKPHPDIFVQ
                     AANQAGYGPAECVYIGDRYVEDIEGPHGVGMPAILKMKAGREYPAEMPLARRRIGALA
                     ELADHLRF"
BASE COUNT          260 a          559 c          483 g          303 t
ORIGIN      
        1 gcccgcacgt gtacatgcgc accttccccg gctcgatcgg ccggaactcc tccttctcgc
       61 ggttcagcgt gttgcggaat cgcaggggca tggatcagac ctccgtgaga ccggccaccg
      121 tccgggcgtc gagtttgcgc accaccgccg cgatgagccg gaccgtgttg tcgtagtcgt
      181 cgcgattgag gatgccgttg tggctgtgga tgtagcggac cggcgggccg accaccaccg
      241 acgggacgcc cgtccgcgac atgtgaatgc gcccggcgtc ggtcccgccc cgctccatgt
      301 aggagaggtg gtacggaatt ttcagctcct ccgcggtgtc ggtgacgaga tcgcgcagcc
      361 ggcggttcgg aatcatcgag gcgtcgaaca gcaggatgcc cgggccgccc cccagcttct
      421 cggccttctt gaaaccgttg ggcgggatgt cctggccgat gccgacatcg acaatgagcg
      481 ccgcgtcggg gtcgcccagg tgggcgagcg tctgcgcccc ccgcagcccg acctcctcct
      541 gcgtcgaacc ggcgccgagg agcgtgttcg ggtgcccctc gcccttgagc gcgcggaaaa
      601 cgtcgatcac caccgcgcag gccatgcggt tgtcgaacgc cttggacaga tacatccgcg
      661 gattccccat gatcgtgaaa tcggaatcgg ggatgatcgg gtcgccgagc tggatgccga
      721 gcgcctcctt ggcgttgaat ccctcctgca cccccacgtc gatgcacatg tctttcagct
      781 cgagcacttt cttgcgctcc tcggcctcca gcatgtgcgg gggcttggag ccgacgaccc
      841 cgatcaccgg acccttgcgg gtgatgaccc gcatccgctg accgagggcc acgtgacccc
      901 accagccgcc cagggggaga aacttgatat acccctcgga tgtgatctcc ctgaccatga
      961 acccgacttc atccatgtgc ccggccacca tgacccgcgg gcggtcggcc gttcccttct
     1021 tccaacccat gatcgagccc agccggtcat actcgatccg atccgcagtc tctctcaact
     1081 cgcgcgccat caggcggcgc acttcttcct cataccccga cacgccgttg gcttcggtga
     1141 tctctttcag gagttgctcg gtcctgtcca cgatatcctc atttcctcta tagcggtctt
     1201 ctcgcgtcat cagcttcggt ttgcgcccgc aaggcgacaa tatagccggc ccgcggctgc
     1261 tcgtaaaccc ctaaaagcga agatggtcgg ccagttccgc cagggctccg atccgacggc
     1321 gggccagcgg catctccgcc ggatactccc gtcccgcttt catcttcaaa atcgccggca
     1381 tccccacccc gtgcggtccc tcgatgtcct ccacgtaccg gtcgccgatg tagacgcatt
     1441 ccgccggccc gtacccggcc tggttggccg cctggacaaa gatgtcgggg tgcggcttgc
     1501 gcttgccgaa ggtggaggag aagatggtga aatcgaagaa gcggctcagc ccgaaccgcc
     1561 ggagttcccg caggtgcacg tcttccggaa acacggtgtt ggaga
//
LOCUS       DAOOKS010000520         1595 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_100571_length_1595_cov_3.014286, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000520 DAOOKS010000000
VERSION     DAOOKS010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100571_length_1595_cov_3.014286"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="PK112_08080"
     CDS             <1..293
                     /locus_tag="PK112_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HPC12041.1"
                     /translation="MNLLPIGQFDGGHVLYGLFRRAQHVLGMIGLAALLVLGYFSPFW
                     WLFGAFGLLFGVKHPPTLDDDRPLSPTARRMGIAAIVIMALSFTPMPFPSPF"
     gene            complement(262..>1595)
                     /locus_tag="PK112_08085"
     CDS             complement(262..>1595)
                     /locus_tag="PK112_08085"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPC12042.1"
                     /translation="LPKVREMRAGYPDAVVILMTAHPTVQTAISVLRRGAYDFLVKPF
                     KLELLKSTIERALEHQRLARENVRLRGQVEFLKVANAHKAHIDIERYLRLVIESCKKE
                     LGAVAAGIIEVDPSSGDLLRKLCVVDDPLLEPLALDPTVLEKFSSAGRAEPVIEARRT
                     QSKAGREVRLSILQPIMVRQRFHGVITLLVVSKFGRVTPGQLDVLAILTNSAAAAIAN
                     DKLYRDLRRSYLEAIRGLANAIEARDKYTAGHTDRVSRLAEMIARRLGWSESRIFDLH
                     MGCTLHDIGKIGVPDRILNKPTALTEEERTQMRSHPEVGLRIIRGIELFKPAVPYIKA
                     HHEWYDGTGYPKGLRGEEIPVEGRLLAVADTFDAILSDRPYRNGNSLAVAVRELLTYR
                     GRQFDPEIVDVFLEILREGQIDFRRMYGRSEDVSCLIDEARAKREKETASA"
BASE COUNT          234 a          481 c          594 g          286 t
ORIGIN      
        1 cgatgaatct cctgccgatc gggcagttcg acggcgggca cgtcctctac gggttgttcc
       61 ggcgggcgca gcacgtcctg ggcatgatcg gcctggcggc gctgctcgtg ctcggctact
      121 tctccccctt ctggtggctc ttcggcgcgt tcggactgct cttcggcgtg aaacacccgc
      181 cgacgctcga cgacgaccgg ccgctgagcc cgaccgcgcg gcgcatgggc atcgccgcca
      241 tcgtcatcat ggcgctgtcg tttacgccga tgccgtttcc ttctcccttt tagcgcgcgc
      301 ctcgtcgatg aggcaggaga cgtcttcgct gcgcccgtac atccggcgga agtcgatctg
      361 gccctcccgc agaatttcca ggaaaacgtc gacgatctcg gggtcgaact ggcggccgcg
      421 gtaggtgagg agttcgcgga cggcgacggc gagggagttg ccgttgcggt agggacggtc
      481 cgagaggatg gcgtcgaagg tgtcggcgac ggcgaggagg cggccctcga cggggatctc
      541 ttcgccccgg agccccttgg gatagccggt gccgtcgtac cactcgtggt gggccttgat
      601 gtacgggacg gcgggtttga agagctcgat gccgcggatg atgcgcaggc cgacttcggg
      661 gtggctgcgc atctgggtcc gctcctcctc ggtcagggcg gtgggtttgt tgaggatgcg
      721 gtcgggcacg ccgatcttgc cgatgtcgtg gagggtgcag cccatgtgga gatcgaagat
      781 gcgcgattcg ctccagccga ggcggcgggc gatcatctcg gccagccggg agacgcggtc
      841 ggtgtgcccg gcggtgtact tgtcgcgggc ctcgatggcg ttggccaggc cgcggatcgc
      901 ctcgaggtag gagcgccgca ggtcgcggta gagcttgtcg ttggcgatgg cggccgccgc
      961 cgagttggtg agaatggcga ggacgtcaag ctgcccgggg gtgacgcgcc cgaacttcga
     1021 caccaccagc agggtgatca cgccgtggaa acgctggcgg accatgatcg gctggaggat
     1081 ggagaggcgc acctcccgcc cggctttgga ctgcgtccgg cgggcctcga tgaccggctc
     1141 ggcccgtccg gcggaggaga acttctccag caccgtggga tccagcgcga gcggctcaag
     1201 gagcgggtca tcgacgacac agagcttacg cagcaggtcg cccgaactgg ggtcgacttc
     1261 gataatgccg gcggcgacgg cgccgagctc cttcttgcac gactcgatca cgaggcgcag
     1321 gtagcgttcg atgtcaatgt gcgccttgtg ggcgttggcc actttcagaa actcgacctg
     1381 gccgcggagc cggacgttct cgcgggcgag acgctggtgc tccagggcgc gctcgatcgt
     1441 cgatttgagc agttcgagct tgaagggctt gacgagaaaa tcataggcgc cgcggcgcag
     1501 cacggagatg gcggtctgga cggtcgggtg ggcggtcatc aggatcacca cggcgtcggg
     1561 gtagccggcc cgcatctcgc gcaccttggg caggg
//
LOCUS       DAOOKS010000521         1595 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_100584_length_1595_cov_2.754545, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000521 DAOOKS010000000
VERSION     DAOOKS010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100584_length_1595_cov_2.754545"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="PK112_08090"
     CDS             <1..471
                     /locus_tag="PK112_08090"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HPC12043.1"
                     /translation="GGTSVAVDANQNAVFGYSPCWLHTVKYSPEGDLLWWRPFAVDSA
                     GLGTFRDLAADQDGNVYVIGEVPQQVSGTEWLEDIVLYKYPASCCLLRGDMNESGGVT
                     VADITFLIGYVFRGGPPPYCLTEADVSGDGHVTVVDVTSLIAYVFRGGPAPAGC"
     gene            complement(507..941)
                     /gene="rfaE2"
                     /locus_tag="PK112_08095"
     CDS             complement(507..941)
                     /gene="rfaE2"
                     /locus_tag="PK112_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HPC12044.1"
                     /translation="MAARHRRRGHKIVFTNGVFDILHRGHVEYLQKAKAMGDILIVGL
                     NSDASTRRLKGPERPVQSQADRAAVLLALRAVDYVVIFGEDTPYELIRQVRPDVLVKG
                     SDYKLGEIVGRDIVRSHGGTVRRVKLTPGRSTTQVLRKAAKG"
     gene            complement(1013..>1595)
                     /locus_tag="PK112_08100"
     CDS             complement(1013..>1595)
                     /locus_tag="PK112_08100"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HPC12045.1"
                     /translation="ESVNAVIISDYGKGVITLSLLNSVMEVCRKRGLFVAVDPKETHF
                     HNYKRVSLITPNHHEAGFAAGRRIVSEADLIDVGNSLLRELEARSILITRGPDGMSLF
                     EQDAEPTHIPTFARKVFDVTGAGDTVIAAFVAAVCAGATLVEAAIVANAGAGITVGEV
                     GTATVSSAELYAELLRNVKNGTLVTRGSTGTQA"
BASE COUNT          256 a          560 c          470 g          309 t
ORIGIN      
        1 ggcggtacgt ccgtggctgt cgatgcgaac caaaacgctg tcttcggtta ctccccctgc
       61 tggctccata ccgtgaaata ctctcccgag ggcgacctgc tctggtggcg tcccttcgcg
      121 gtagactcag cgggcttagg gaccttccgg gatctcgccg ccgaccagga tggcaacgtg
      181 tacgtgatcg gggaggtgcc gcagcaggta tccggcaccg aatggctcga ggacatcgtt
      241 ctctacaaat accccgcctc ctgctgcctg ctgcgcggag acatgaatga aagcggcggc
      301 gtcaccgtgg ccgatataac gttcctcatc ggatacgttt tccgcggcgg accgcctccc
      361 tactgcctca ctgaagcgga cgtgtccggc gacgggcacg tcacggtcgt cgatgtcacg
      421 tccctgatcg cgtacgtatt tcgcggcggt ccggccccgg ccggctgctg aaaccggcgc
      481 ggcgggagtc ctcccgactc ccgcctctac cctttcgcgg ctttccgcaa aacctgcgtg
      541 gtcgaccgcc ccggcgtcag tttcacgcgc cgcacggtgc cgccgtgcga ccgcacgata
      601 tccctcccca cgatttcgcc caacttgtag tccgatccct tcaccaagac atccggccgc
      661 acctgcctga tcaattcgta cggcgtgtcc tcgccgaaaa tgacgacgta atcgacagcg
      721 cggagggcca ggagaacggc ggcccgatcc gcctgactct ggaccggccg ttccggtcct
      781 ttgagacggc gcgtcgaagc atccgagttc agccccacta tcagtatgtc ccccatagcc
      841 ttggcttttt gaaggtactc cacgtgcccg cggtggagga tatcgaacac gccgttggtg
      901 aagacaattt tgtggccgcg ccgccggtgg cgggccgcca cctcgccgat cctcctgcgc
      961 cctgtcggcc tggccctgtt cattgcgccg cccgtcggac ctttctcccg tgctacgcct
     1021 gcgtccccgt cgatcccctg gtcaccagcg tcccgttctt gacgttgcgc agcagttccg
     1081 cgtagagctc tgcagaggac acggtcgcgg ttccgacctc tccgacggtg atgcccgccc
     1141 cggcgttcgc gacaatcgcc gcctcgacca gcgtcgcccc cgcgcacacg gcggccacga
     1201 aagccgcgat cacggtatcg ccggccccgg tcacatcgaa cactttccgg gcgaaggtcg
     1261 gtatgtgggt cggttcggcg tcctgctcga acagcgacat accgtccggc ccgcgggtga
     1321 tgagaatcga ccgggcctcg agttcccgca gcaggctgtt cccgacatcg atcaggtcgg
     1381 cctcggatac tatccgccgc ccggcggcga acccggcctc gtggtggttc ggggtgataa
     1441 gcgacacgcg tttatagttg tggaaatgcg tttctttggg gtcgacggcc acaaacagcc
     1501 cccgcttccg gcaaacctcc atcacgctgt tcagcagcga cagcgtaatg acgcccttgc
     1561 cgtagtcgga gataatcacg gcgttgaccg actct
//
LOCUS       DAOOKS010000522         1577 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_102267_length_1577_cov_3.180683, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000522 DAOOKS010000000
VERSION     DAOOKS010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102267_length_1577_cov_3.180683"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..876
                     /locus_tag="PK112_08105"
     CDS             100..876
                     /locus_tag="PK112_08105"
                     /inference="COORDINATES: protein motif:HMM:NF024783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HPC12046.1"
                     /translation="MPTSKRERWSRDQLLIAFNLYCRLPFGRLHSRHPDVIRAAELIG
                     RSPDALAMKLVNFASLDPQHVARGVRGLNNVSRADRDMWREFSGNWEALAYESETAWR
                     ALLTGQDHGREGEEWEAPEGVTEKVMSTRVRVVQGFFRRTVLAAYEYRCACCRLSIQQ
                     MLNASHIIPWRVDKQRRADPTNGIAMCAFHDRAFDRGLITLDGDRRIVVSREVPTKSR
                     VAMHRVGLIELAGARAAAPSRFAPDEAALEYHRENIFTGG"
     gene            complement(1125..>1577)
                     /locus_tag="PK112_08110"
     CDS             complement(1125..>1577)
                     /locus_tag="PK112_08110"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HPC12047.1"
                     /translation="QVGADRIANAVAALARWGGPAIVVDLGTATTFDVLDAAQAYIGG
                     VIIAGPEAAMAELARRAARLFEVRIEKPDTVVGKSTAGALKSGAFYGLIGQVDFIIDR
                     IIEECGFDRCKVAATGGLAFEIEGHSRHITEVVPSLTLEGLRIIGEGN"
BASE COUNT          301 a          424 c          580 g          272 t
ORIGIN      
        1 ggaggcggac gaggggggcg gggagtaggg ggggcttctg ttctatcaag agggcggagc
       61 acgcttaaag atgcaatctg cccggggagg aggacgggtg tgccgacatc aaagcgggaa
      121 cgctggtcca gagatcagct gttgattgcg tttaatcttt actgccgcct gccgttcggg
      181 cggcttcaca gcagacaccc ggatgtgata cgcgccgccg aattgatcgg tcggtccccc
      241 gacgcgcttg cgatgaaact ggtcaacttc gcaagtcttg atccgcagca tgtagcacgc
      301 ggggtccggg gactgaacaa cgtcagccgg gccgatcggg atatgtggcg ggagttttcg
      361 ggaaactggg aggcgcttgc gtatgagagc gagacagcct ggcgagcgct gctgaccggg
      421 caggatcacg ggcgggaagg tgaagagtgg gaagcgccgg agggagtgac cgagaaggtc
      481 atgtccaccc gggtacgggt ggtgcagggg tttttccgac gaacggttct tgcggcttat
      541 gagtaccgat gcgcctgttg tagattgtcc attcagcaga tgctgaatgc aagccacatc
      601 attccgtggc gggttgacaa acaacggcgg gccgacccga ccaacggcat tgccatgtgc
      661 gcgtttcacg atcgggcgtt cgacagaggg ttgatcaccc tcgacggcga taggcgcatc
      721 gtggtatcga gagaagtgcc gacgaagtcg cgggtcgcta tgcacagagt tggcttgatc
      781 gaactagccg gcgctcgggc cgcagcgccg tcgagattcg cgccggatga agcggcgctg
      841 gagtaccacc gagagaacat tttcaccggc ggataggagg gaaaagatgt accggacgtt
      901 caggttaacg cttctggagg ctgatgcacg gcgggtgctg gacgctcttc ggatgcagga
      961 ggagttgtgg gagcgaaccg ctcgatatct gcgagaagac tacctcgatt cggacgaagg
     1021 ctgcgtggct gattgcgggg atgagtggga ggctgcagaa atccatcggc actacgccgg
     1081 aatcatcacg gctctggaag ggcagatcga cgaacagcgg gcgcctagtt cccctcgccg
     1141 atgattcgca agccctcgag cgtgagcgag gggaccacct cggtgatgtg gcgggagtgg
     1201 ccctcgatct cgaaggccag gccgccggtc gcggccacct tgcagcggtc gaagccgcac
     1261 tcctcgatga tgcggtcgat gataaaatcg acctggccga tcaggccgta gaaggcgccg
     1321 gatttgagcg cgccggcggt cgatttcccc acgaccgtgt cgggcttctc gatgcggacc
     1381 tcgaagaggc gggcggcgcg gcgcgccagc tccgccatcg ccgcctccgg accggcgatg
     1441 atgacgccgc cgatgtaggc ctgggcggcg tcgaggacgt cgaaggtggt cgcggttccc
     1501 aggtcgacga cgatggcggg gccgccccag cgggcgaggg cggcgacagc gttggcgatg
     1561 cggtcggcgc cgacctg
//
LOCUS       DAOOKS010000523         1576 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_102371_length_1576_cov_3.263642, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000523 DAOOKS010000000
VERSION     DAOOKS010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102371_length_1576_cov_3.263642"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="PK112_08115"
     CDS             complement(<1..370)
                     /locus_tag="PK112_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12048.1"
                     /translation="MNRPLVVLLALACAVAPAVASADPAAPTALPDTPAGRCLAEFVS
                     ALNSSDSAQWIAFLAQHPPADANDSATVQERRFGLMRTVHGDFGGLDPVKVLESSEYF
                     LSVILRGRVPGLEFQDPLRQL"
     gene            complement(523..>1576)
                     /gene="ric"
                     /locus_tag="PK112_08120"
     CDS             complement(523..>1576)
                     /gene="ric"
                     /locus_tag="PK112_08120"
                     /inference="COORDINATES: protein motif:HMM:TIGR03652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HPC12049.1"
                     /translation="RAAEGMPYTGLQPAGLPAPAEVAAADKAYAADDVEPVIELLESS
                     LEEHVRSLFTEAMRAKKEGDKSLEAGREKIDAYVRFILYVHGLVEKIEAGPEHGVGEH
                     SSVPDPHNTKGATMNTVSADRTVGDLVTEQPGRAKVFEDLGIDYCCGGHRPLTEACAE
                     KGIDPENVLAQLARVDAARRPDADADYGSMSLTALADHIVDTHHAYLRQALPRANQLA
                     AKVAQAHAASPKGADLKRVDEHVRALAAELQSHMVKEEHILFPMIRRIDAGERGLVID
                     GPVMVMMREHDEAGEALAAIRTLTDDFALPAWGCNTYRAMLDALQELELDLHQHIHKE
                     NNVLFPRAQQAAAVGA"
BASE COUNT          246 a          501 c          569 g          260 t
ORIGIN      
        1 cgagctggcg cagcgggtcc tggaactcga gcccggggac gcggccgcgc aggatgacgg
       61 agagaaagta ctccgacgat tcgagaacct tgaccgggtc gagcccgccg aaatcgccgt
      121 ggaccgtccg catcagcccg aaacggcgct cctggacggt ggcgctgtca ttcgcatccg
      181 ccggcggatg ctgggccaaa aaggcaatcc actgcgcgct gtcgctgctg ttcagtgcgg
      241 acacgaattc ggccagacag cggccggcgg gagtatcggg cagagccgtc ggagcggcgg
      301 gatccgcgga cgcgaccgcg ggtgcgacgg cgcaggcgag cgcaagaaga accacgagcg
      361 gacggttcat atctcctccg tcgagcggtt tccgccagta tacgaaacgc gcgggccggt
      421 tgttgcagaa aacgcggcgg ggatgggacc gaccgggccg cgaaagaaca aaaacggcgc
      481 cggcggatcg gccgccggcg ccggtgtcgg ctgtgtgagc gttcaggcgc cgacggcggc
      541 cgcctgctgc gcccgaggaa agaggacgtt gttctccttg tgaatgtgct ggtggagatc
      601 cagctccagc tcctggaggg cgtcaagcat cgcccggtag gtgttgcatc cccacgcggg
      661 gagggcgaaa tcatcggtga gcgtgcgaat ggcggcgagc gcctcgcccg cctcgtcgtg
      721 ctcccgcatc atgaccatca ccggaccgtc gatcaccagg ccgcgctccc cggcgtcgat
      781 gcgccggatc atgggaaaga gaatgtgctc ctccttcacc atgtgcgact ggagttcggc
      841 ggccagggcg cgcacgtgct cgtcgacgcg cttcaggtcg gcgcctttgg gcgaggcggc
      901 gtgggcctgg gcgactttcg cggccagttg gttcgcgcgc ggcagcgcct ggcgcaggta
      961 ggcgtgatgg gtgtcgacga tgtgatcggc cagcgctgtg agactcatcg aaccatagtc
     1021 cgcgtcggcg tcggggcgcc gcgccgcatc gacccgggcg agttgagcga gaacgttttc
     1081 agggtcgatc cccttctccg cgcaggcctc ggtcagcggc cggtgcccgc cgcagcagta
     1141 gtcgatgccg agatcctcaa agaccttggc gcggccgggc tgttcggtga ccagatcgcc
     1201 gaccgtccgg tcggcagaaa cggtattcat agttgctcct ttcgtattgt gcgggtcggg
     1261 gaccgacgag tgttcgccca cgccgtgctc cggcccggcc tcgattttct cgaccaggcc
     1321 gtgcacgtag agaatgaagc ggacgtaggc gtcgatcttc tcccgccccg cctccagtga
     1381 cttgtctccc tccttcttgg cgcgcatcgc ctcggtgaag aggctgcgga catgttcctc
     1441 cagcgacgat tcgagcagct cgatcacggg ctccacgtcg tcggcggcgt aggccttgtc
     1501 ggccgcggcc acttcggcgg gggcggggag gccggcgggc tgcaggccgg tgtagggcat
     1561 gccttcggcc gcgcgg
//
LOCUS       DAOOKS010000524         1569 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_103084_length_1569_cov_2.752972, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000524 DAOOKS010000000
VERSION     DAOOKS010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103084_length_1569_cov_2.752972"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..731)
                     /locus_tag="PK112_08125"
     CDS             complement(132..731)
                     /locus_tag="PK112_08125"
                     /inference="COORDINATES: protein motif:HMM:NF017415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HPC12050.1"
                     /translation="MRLIKRYSNRRLYDTRASRTITHRELAALVRRGEALRVVDTVTG
                     SDITAEVLGRIAFRETAAWTTARESQALFTRIIELGGERTMSILKNTVLASIGAFEVS
                     KARAEKIVDELIKRGEVEKGDKTKAVMEILDRAEKSTAKVYGKVAAEVRKVQQEVAKY
                     SDQAKKYKLVKTEDLRALEEKVERLTRLIEDMEARSGKS"
     gene            complement(810..1211)
                     /locus_tag="PK112_08130"
     CDS             complement(810..1211)
                     /locus_tag="PK112_08130"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPC12051.1"
                     /translation="MSHKINISSAKVLVIDDEPEITEIVETFLTEAGYYVMVENQPFR
                     AIEKATAFNPDVILLDIMMPGMDGYDVCEALKKVPRLAGTPIIFLTGKDRNDDMGRSF
                     KSGGDMFIKKPFSCERLLEILNIVLLSTGRH"
     gene            1441..>1569
                     /locus_tag="PK112_08135"
     CDS             1441..>1569
                     /locus_tag="PK112_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011692503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein"
                     /protein_id="HPC12052.1"
                     /translation="MKEAADRKYIVRNRKARHDYEIKETYEAGIALVGSEVKALREG"
BASE COUNT          251 a          442 c          549 g          327 t
ORIGIN      
        1 gagggttgcg ggcgccccgg gcgcgcgcgt atcatctagc ggtctcgtct cactgtggag
       61 agaccaggta cactgagccg gcggccctgc ggccgccggc gtcgttatgg gggtcgggct
      121 gaggcgggcg gtcaggattt gcccgaccgg gcttccatgt cctcgatcag ccgcgtcagc
      181 cgctcgacct tttcctcgag ggcgcggagg tcctcggttt tgaccagctt gtacttcttg
      241 gcctggtcgc tgtacttggc gacttcctgc tgcactttcc tgacctcggc ggcgactttg
      301 ccgtagacct tggcggtcga cttctcggcc ctgtcgagga tttccatgac cgccttggtt
      361 ttgtcgccct tctcgacctc gccgcgcttg atgagttcgt cgacgatttt ttcggcgcgg
      421 gccttgctga cctcaaaagc gccgatcgag gcgaggacag tgttcttgag gatgctcatg
      481 gttctctcac ctccaagttc gatgatccgc gtgaaaagag cctgggactc ccgggccgtg
      541 gtccaggcgg cggtttcgcg gaaggcaatg cggccgagca cctcggcggt gatgtcgctg
      601 ccggtgacgg tgtcgaccac ccggagcgct tcgccgcggc gcaccagcgc ggcgagttcg
      661 cggtgggtga tggttcggct ggcgcgggtg tcgtagaggc gccggttgct gtaccgtttg
      721 atgagccgca tagaccacct gccctattat agtgcggcgc accaaaaaga gcaacaaaaa
      781 tcggcggccg gagaaagcgg ggccgcgggt cagtgccggc cggtggagag caggacaatg
      841 ttgaggattt ccaggagccg ttcgcacgag aacggtttct tgatgaacat gtctccgccc
      901 gatttgaagg atcgccccat atcgtcgttg cggtctttcc cggtgaggaa gatgatgggg
      961 gtgccggcga ggcgcggaac cttcttgagc gcttcgcaga catcgtaccc gtccatgccg
     1021 ggcatcatga tgtccagcag gatgacatcg ggattgaagg cggtggcttt ttcgatggcc
     1081 cggaagggct ggttttccac catgacgtag tagccggctt cggtgaggaa ggtttcgacg
     1141 atttcggtga tttcgggttc gtcgtcgatg accagcacct tggctgacga tatgttgatc
     1201 ttgtgagaca tttgtccacc tttgtgaagt ctccgcccgg ggccgagggg ccgtcgggcg
     1261 ctacatattt gttttcggct gtcggggggg aacactttac cggggggcgg caatttcccc
     1321 ggaggagcgg ggggcgcgcg gcggcgcggg cggccgcggc ggcgatgcgg ggacgacgca
     1381 aacggcgggg gagacccgat tttaattgcc cccgctggtc ggccgtccta tcgtacgccc
     1441 atgaaggaag ccgccgaccg gaaatatatc gtccgcaacc ggaaagcccg ccacgactac
     1501 gagatcaagg agacgtacga ggcgggcatc gccctggtgg gtagcgaggt caaagcgctg
     1561 cgggagggg
//
LOCUS       DAOOKS010000525         1567 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_103259_length_1567_cov_3.585317, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000525 DAOOKS010000000
VERSION     DAOOKS010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103259_length_1567_cov_3.585317"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(411..>1567)
                     /locus_tag="PK112_08140"
     CDS             complement(411..>1567)
                     /locus_tag="PK112_08140"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPC12053.1"
                     /translation="QDRMIRIAFVIDTIATPGAGTEKQLLLLVRHLDRSRFEPHLLCL
                     QSSPWLERNSLPCPVEIIGVRSLRSPATTGALLRFRRYVRRHRIDIVQTFFIDSNLFG
                     TVSGWLSGVPVIISSRRNFGKDYWHTPAWLFLLRLLRRLTTCYIANSRLTADYSIEVE
                     RIAADRMHVIYNGLDFAPFANLTPELRAAARSAWGVADRHVLIGIIANLRPIKNHRLF
                     VEAAARVHSRRPDTRFLIAGDGDQREPIECRLHELGLQDTAILTGQVADVLPVLAALD
                     IGVLCSHGESLSNAIIEYMAAGIPPVVSDVGGNREAVGGEHGLVFPPDDLDAFVACLD
                     RLIADPALRTRLGRAAADYARCTYEYREAVGRHETLYETCRAAARPRRRS"
BASE COUNT          284 a          409 c          645 g          229 t
ORIGIN      
        1 aggtcggcgg tggcggcgaa gccggggctg tcggccaggt agtcggggag ccagatgaag
       61 gcgtggccgc ggtagagttt gtaggtcatg gcgccgccgg cgaggagccc gacgatgacg
      121 accgcaatga gcgcggtgcg gaagagcttc attcgctgtc tatctccttc agtgagcggc
      181 cgggtacatc tgtcctgtta aggccgaggg ggacgtctct tacacgcaga ccgccgatgc
      241 gttcctgctg tgcaaaccgc gggcgcgggg gataagttcc ccgattcagg aaggccgccg
      301 gcagacgact tcggcgatca tcaaaaagcc acttctcaat atattggcgg ggccatggag
      361 agcaaggcaa atcaacgact cgggacgagg cgcgcaaaag ggtcggggga tcaggaacgg
      421 cggcgggggc gggccgcggc gcggcaggtt tcgtagaggg tctcgtggcg gccgacggcc
      481 tcgcggtatt cataagtgca gcgggcgtaa tcggcggcag cgcggccgag gcgggtccgg
      541 agggcggggt cggcgatgag gcggtcgagg caggcgacga aggcgtcgag gtcatcgggt
      601 ggaaacacca ggccatgttc gccgccgacg gcctcacggt tgccgccgac gtcggacacc
      661 accgggggga tgccggcggc catgtactcg atgatggcgt tggagaggct ctcgccgtgc
      721 gagcagagga cgccgatgtc gagagccgcc aggacgggga ggacgtcggc gacctggccg
      781 gtgaggatgg cggtgtcctg caggccgagc tcgtggaggc ggcactcgat gggttcgcgc
      841 tggtcgccgt cgccggcgat caggaaccgc gtgtcggggc ggcgggagtg gacgcgggcg
      901 gcggcctcga cgaagaggcg gtggttcttg atggggcgga ggttggcgat gatgccgatg
      961 aggacgtggc ggtcggccac gccccaggcc gaccgcgcgg cggcgcgcaa ttcgggcgtc
     1021 aggttcgcga acggagcgaa atcaagaccg ttgtaaatga cgtgcatgcg gtcggcggcg
     1081 atccgctcaa cttcgatcga gtagtcggct gtcaggcggg agttcgcgat gtaacaggtg
     1141 gtcaggcggc gaagcaggcg gaggaggaaa agccaggccg gggtgtgcca gtagtccttg
     1201 ccgaaattgc ggcggctgga gatgatgacg ggaacccccg aaagccagcc ggagacggtg
     1261 ccgaagaggt tggaatcgat gaagaaagtc tggacgatgt cgatgcggtg ccggcggaca
     1321 tagcggcgga accgcagcag cgcccctgtc gtcgcggggg agcggagcga gcggacgccg
     1381 atgatttcaa ccgggcaggg cagcgagttt cgctccagcc agggggagga ctggaggcac
     1441 aggaggtgcg gttcgaagcg ggagcggtcg aggtggcgca cgaggagcag aagctgcttc
     1501 tcggtgccgg cgccgggggt ggcgatggtg tcgatgacga aggcgatgcg gatcatacgg
     1561 tcctgtc
//
LOCUS       DAOOKS010000526         1560 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_104004_length_1560_cov_2.411296, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000526 DAOOKS010000000
VERSION     DAOOKS010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104004_length_1560_cov_2.411296"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..928)
                     /locus_tag="PK112_08145"
     CDS             complement(<1..928)
                     /locus_tag="PK112_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12054.1"
                     /translation="MPARARERAEALLWLIPALALYALFVLRLEFVQDDAFISFRYVA
                     NFLNGHGLVYNIGERVEGYTSPGWVVLLSLAGALGFDYPAVARFLGILCGAGLPVLAW
                     AVARRTEPQRGPLAAAIPAYLVAATASLAYWAAAGLETTAFALAAGVSLYGFLVRRVP
                     LLAVGLVWAVWLRPEGAVVAAILIAVETLVIKRFPRMTVAAGALAFVLSLPMVAFKLG
                     YYGALPPNSFYAKTGWDFEQIRGGAEYAGQFFTHYPVAAFGLLAGLVTWRASGPTVRA
                     IGLFSLFYVVYVILIGGDVLKVHRFFLPILGPL"
     gene            complement(1038..>1560)
                     /locus_tag="PK112_08150"
     CDS             complement(1038..>1560)
                     /locus_tag="PK112_08150"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPC12055.1"
                     /translation="DQKGFDLIAEAADRIFAMNLQMIILGTGETRYHDFLTRLQAAYR
                     DKVRAYLMFDDTLAHQIEAAADIFLMPSRFEPSGLNQLYSLKYGTVPIVRRVGGLADT
                     VTDYDPATGEGTGFVFDDYTAEALTGAIARAVLLFERKRVWTKVMKRGMAEDYSWQHT
                     AEEYARLYRDLVS"
BASE COUNT          305 a          513 c          522 g          220 t
ORIGIN      
        1 tcaggggccc cagaatcggc aggaagaagc ggtggacttt cagcacatcc cccccgatca
       61 ggatcacata cacgacatag aacagcgaga acagcccgat cgcgcggacg gtcggcccgg
      121 aggcccgcca cgtgaccagc ccggctagca gtccgaaggc cgccaccggg tagtgagtga
      181 aaaactgccc ggcgtactcc gctcccccgc ggatctgctc gaaatcccag ccggtcttgg
      241 cgtagaacga attcggcgga agggcgccgt agtaccccaa tttaaacgcg accatcggca
      301 gagaaaggac gaaagcgaga gcgccggcgg ccacggtcat gcggggaaag cgcttgatga
      361 cgagggtttc gacggcgatg aggatggcgg cgaccacggc gccctccggg cgcagccaga
      421 ccgcccacac cagaccgacc gccagcagcg gcacgcggcg caccaggaac ccatacaggc
      481 tcacgccggc ggccagcgcg aaagccgtgg tttccaaacc ggccgcggcc cagtaggcca
      541 gcgaggccgt ggccgccacc agataggccg ggatcgcggc cgcaagcggg ccccgctgcg
      601 gctctgtccg acgcgccacc gcccatgcga gaaccgggag cccggcgccg cagaggatgc
      661 cgaggaagcg ggcgaccgcg gggtagtcga atccgagggc gccggccagc gacaggagca
      721 ccacccagcc ggggctcgtg tacccctcga cccgctcccc gatattgtac acgagaccgt
      781 gtccgttcag gaagttggcg acgtagcgaa aggaaatgaa ggcgtcgtcc tgaacgaatt
      841 ccaatcgaag gacgaaaagt gcgtagagcg ccagggccgg aatgagccaa aggagcgctt
      901 ccgcacgttc gcgcgctcgc gcgggcatgg tgctgtcatc tccgctcccg ccccgagatt
      961 gtgctcgggc gggtcgtgaa agacggcccg gtgcggcccg ggtcgtcgcg gcaattactt
     1021 cttgcgccgg accggtctca cgagaccagg tctcgataga gccgggcgta ctcctcggcc
     1081 gtgtgttgcc acgagtaatc ctcggccatg ccgcgtttca tcactttggt ccagacgcgc
     1141 ttgcgttcga acagcaacac ggcgcgggcg atggcccccg tgagcgcctc ggcggtgtag
     1201 tcgtcgaaca caaagccggt cccctcgccg gtcgccgggt cgtaatcggt caccgtatcg
     1261 gccaggccgc ccacccgccg cacgatcggg accgttccgt acttgagcga atacaactgg
     1321 ttcaggccgc tcggctcgaa ccgcgagggc atgaggaaga tgtcggccgc ggcctcgatc
     1381 tggtgggcga gggtgtcgtc gaacatgagg tacgcccgga ccttgtcgcg gtaggccgcc
     1441 tgcaaacggg tgagaaaatc gtggtagcgg gtttcacccg tccccaggat aatcatctgc
     1501 aggttcatgg cgaagatccg gtcggccgcc tcggcgatga gatcgaatcc cttctggtcg
//
LOCUS       DAOOKS010000527         1557 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_104259_length_1557_cov_4.336218, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000527 DAOOKS010000000
VERSION     DAOOKS010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104259_length_1557_cov_4.336218"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="PK112_08155"
     CDS             complement(<1..576)
                     /locus_tag="PK112_08155"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HPC12056.1"
                     /translation="MMRSLRCATVLLLLVLCASTVVAVNEHYLRLKADSPKALAELSE
                     VVSIDNVQDGVVLAYATDEQLAALERLGYAFEELPRPSTLIVPEMTSDLRDARAWDVY
                     PTYDAYVAMMNQFAATYPGLCQIVTIGTTTEGRALLAAKISDNVAVDEDEPEVLYLSS
                     MHGDETTGYVLMLRMIDSLLSAYGVNPQITSM"
     gene            complement(699..>1557)
                     /locus_tag="PK112_08160"
     CDS             complement(699..>1557)
                     /locus_tag="PK112_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12057.1"
                     /translation="FPFYDSAYTEIYIGSNGIIGFAPGAMNSRFKKHIPNDTLPNALL
                     AWLWDDLNPDDVNNAAAAHVYLDTSGGRCVIQFTAYPEYLANPGDVITAEVILEPDGS
                     IRFQYLSVAAGFDAAGGTIGIESPDGTDGLEVAYLTPYLKDSLCIRFVQPYQWLGLSR
                     FSGTVAAGGTDTVGLMFRSAGLDSGLYQAALVVSCNDPNHPAETVPAELTVTLQPAWI
                     CGDINGDGAGPSVADLTFFINFLFRGGPAPPDPAACDFDGSGDIALPDLTQLINFLFR
                     GGSPPTCGN"
BASE COUNT          299 a          462 c          525 g          271 t
ORIGIN      
        1 catgctggtg atctgaggat tgacgccgta ggcgctcagg agggagtcga tcatgcgcag
       61 catcaggacg tacccggtgg tctcgtcgcc gtgcatcgac gacagataca gcacttccgg
      121 ctcgtcctcg tcgaccgcga cgttgtcgga gatcttggcg gccaacaggg cgcggccctc
      181 ggtcgtcgtg ccgatggtca cgatttggca gaggcccggg taggttgcgg caaactggtt
      241 catcatggcc acgtaggcat catacgtggg gtacacatcc caggcccttg catcgcggag
      301 atcggacgtc atttcgggga cgatcagcgt gctcgggcgc gggagttcct cgaaggcata
      361 cccgaggcgc tccagcgcag ccagctgctc gtcggtggcg tacgccagca cgacgccgtc
      421 ctgcacgttg tcgatcgaga cgacctcgga gagctcggcc agtgccttcg gggaatcggc
      481 cttgagccgc aggtagtgct cgttgacggc gacgacggtc gacgcgcaga ggacaagcaa
      541 aagcaggacg gttgcacagc gaagagaacg catcataact gctttccttt ggttcggcgg
      601 agcctatgta agtattttcg aacagtacct ccacaatata aaccaaaaaa ccgggcgaca
      661 aaggggtttt tgccgcccgg taaggtcagt cggtgcaatc agttgccgca ggtcggcggc
      721 gaccccccgc ggaagagaaa attgatcaac tgcgtgagat ccggcagcgc gatgtcgccc
      781 gagccgtcga aatcgcaagc cgcgggatcc ggcggcgccg gcccgccccg gaagaggaag
      841 ttgatgaaga aagtcaggtc ggccacgctc ggccccgccc cgtcgccgtt gatgtcgccg
      901 cagatccagg caggctggag cgtcaccgtc agctccgccg gaaccgtctc cgccgggtga
      961 ttcggatcgt tgcagctcac cacgagggcg gcctggtaga ggccggaatc cagacccgcc
     1021 gagcggaaca tcaggcccac ggtatcggta cctccggccg ccaccgttcc cgaaaagcgg
     1081 ctgaggccca gccattggta gggctggacg aaccggatgc agagggagtc tttcaggtag
     1141 ggcgtcaggt acgcaacctc caggccgtcc gtgccgtccg ggctctcgat ccctatcgtg
     1201 ccgccggccg catcgaaccc ggcggcgacg ctcaggtact ggaagcggat ggaaccgtcc
     1261 ggttcgagga taacctccgc cgtgatcacg tcgccggggt tcgccagata ttcgggatag
     1321 gcggtgaact gaatgacgca acggccgccg ctcgtatcga ggtagacgtg ggccgcggcg
     1381 gcattgttca catcatccgg gttcaggtcg tcccacagcc aggcgaggag ggcgttgggg
     1441 agagtgtcgt tggggatgtg tttcttgaag cgggagttca tcgctccggg ggcgaacccg
     1501 atgatcccgt tcgacccgat gtagatctcg gtgtaggcgc tgtcatagaa cgggaag
//
LOCUS       DAOOKS010000528         1551 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_104904_length_1551_cov_2.812166, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000528 DAOOKS010000000
VERSION     DAOOKS010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104904_length_1551_cov_2.812166"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="PK112_08165"
     CDS             complement(<1..432)
                     /locus_tag="PK112_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12058.1"
                     /translation="MGGVYVAVHKALPGSGNLATYRPRLATRIFSTERYADGRETHTL
                     LGRVYKQDREPEQLRNIPRDLKFATIAVEDPADGAAALRDAVSRISTSDGVGVTSLNA
                     AGLCARASLPNSSDLKLPAEFQPQDVYDYRTPLGLAMMALEK"
     gene            complement(1314..>1551)
                     /locus_tag="PK112_08170"
     CDS             complement(1314..>1551)
                     /locus_tag="PK112_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12059.1"
                     /translation="EDRVQILTCALGIAGAALVAQTRTPALMLAGFGCWVVSNIVLIG
                     IEWRAGHPWLAAMFAVYEVTALAGIANTLGWIGG"
BASE COUNT          281 a          492 c          500 g          278 t
ORIGIN      
        1 tttttccagc gccatcatgg ccaggcccag cggcgtgcgg tagtcataga catcctgcgg
       61 ctgaaactcg gccggcagtt tgaggtccga cgaattcggc aggctcgctc gtgcgcacag
      121 gcccgcggcg ttgagcgagg tgacccccac cccgtcggac gtggagatcc ggctcaccgc
      181 gtcgcggagc gccgctgcgc cgtccgccgg gtcctcgacg gcgatggtcg cgaacttcag
      241 atcgcggggg atgttgcgaa gctgctccgg ttcccggtcc tgcttgtaca cccgccccag
      301 aagcgtgtgg gtctcccggc cgtcagcata ccgctcggtg gagaagatac gggtagccag
      361 gcgcggccgg taggtcgcga ggttgccgct gccgggtagg gccttgtgga cggccacata
      421 cacgccgccc acaccagatc ctctggttct gcgacggaaa gtactgcccc gcggtgtttt
      481 cggtgtcgtg gggcctcggc gctgtggggg tcggccagac catcgccacc tgatcctcca
      541 gccgcgaccc gttcccggta cactgggggg aaggggaacc cccgttcgtc gtcacccttg
      601 gcgtacccca ggatgaagac ccgtttccgc cggtgagggg cgccaacttc ttccgcagag
      661 aataatcccc acgtcgcttg gtaaccatct tcttccaggt cgctgaggac tgttgagagt
      721 ccaagcgaga tgtggccttc gacgttctcg aagaaaacga cgcgagggtg gattgctcgg
      781 atctgcgccc ggaagtaggg ccagaggtgc cggggatcgg cttttccggc ccgtttcccg
      841 gcggaactga agggagtaca ggggtacccg ccacagagga tgtccaccat tccgtgaaac
      901 gcttcccggg aagcttcgca gatcgctcca gatcggagca gggggaagga accccgcttg
      961 aacctttgca gccaggtttt cgatggcgaa agcttcgacc tctgagtaag cgaggacgcg
     1021 gcacggtcgg ccaagagctc gctcgatgcc aagttcgagc ccggcgtatc ctgtgcaaaa
     1081 actgatgaga gtgggggagg aaggatccac acgccgtcgc cccctcagat ggtgaggctc
     1141 tccctacggg agagggggtg ccagaagccg taaccgatac cgtattttaa gcacatttta
     1201 tcacactcat tttttctgca tgtgcaggtc tgctcaaaca gcacctgcag gagatctatc
     1261 taattcgttt ggttctcacc ggcataaggg ttccggaggg gcgggcccgg gagtcacccc
     1321 ccgatccagc caagggtgtt cgcgatcccc gcgagcgccg tcacctcata gaccgcgaac
     1381 atcgcggcca gccaggggtg cccggcccgc cactcgatcc cgataaggac aatgttgctc
     1441 acgacccagc acccgaaccc ggcgagcatc agggccgggg tccgggtctg cgccacgagc
     1501 gccgcaccgg cgatccccag agcgcaggtg aggatctgga cgcgatcctc c
//
LOCUS       DAOOKS010000529         1549 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_105125_length_1549_cov_2.670013, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000529 DAOOKS010000000
VERSION     DAOOKS010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105125_length_1549_cov_2.670013"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="PK112_08175"
     CDS             complement(<1..482)
                     /locus_tag="PK112_08175"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPC12060.1"
                     /translation="MYESVVAEIERLRPDFVVTVGDMIEGHTTDTIEMRSRWDEYFAI
                     IRPLTMPVRFTPGNDDISTDAMESTYRARAGEPYYAFDHRGIHFVVLDNSRTESAGEI
                     AQEQLGWLKDDLAKNRSACYTIVFLHKPFWYRTLGSGQPDALHDIFRANGVDAVFTGH
                     "
     gene            complement(858..1466)
                     /locus_tag="PK112_08180"
     CDS             complement(858..1466)
                     /locus_tag="PK112_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12061.1"
                     /translation="MLTILIAAWLVSPLYPLNPVVEDTVNGVNMHFTIGVGGPNVLTD
                     PGLLISAKYEFLLRHPFLLRACADYGFAAMRSKTYPDGALHSMILSAEILLYRGTDAM
                     TGFVGMGPLLYKGYFRFDGLEPTDFVPGAVGLDIAMAPKIGYRATLGLRFNRNFSLEI
                     GMSEARPTIRITGDTGDSRLRTFSQPVRLHDVRMSVGYLVPL"
BASE COUNT          310 a          411 c          557 g          271 t
ORIGIN      
        1 atatgaccgg tgaaaacggc atcgaccccg ttggcccgga agatgtcgtg cagggcgtcc
       61 ggctgcccgc tgcccagtgt ccgataccag aacggcttgt ggagaaagac aatcgtgtag
      121 caggcactgc gattcttcgc cagatcgtct ttcagccagc cgagttgctc ctgggcgatc
      181 tcgccggcgc tctctgtacg gctgttgtcg agcaccacga aatggatgcc gcggtggtcg
      241 aaggcgtagt acggctcccc tgcgcgggcg cgataggttg actccatggc gtctgtgctg
      301 atatcgtcgt tgccgggtgt gaagcggacc ggcatggtga gcggtctgat aatggcgaag
      361 tactcatccc accggctgcg catttcgatc gtatcggtgg tgtgcccctc gatcatgtcg
      421 ccgacggtca ccacgaagtc gggccgcagc cgctcaatct cggcgaccac cgattcgtac
      481 actccgtcct ggtggtcacc ggtacggtcg ccaagaaccg cgaatcgaac cggggcgtcc
      541 gatagatcat cgcatggcgt ctgggctgga gctggagatc cgagggcgat gaggacaagg
      601 ccaaatatgg cggtagtttt catcacgtcg caacacccgt ctttgagttt gccggttttg
      661 ccccgaaaga tacaccgggt ccgcgtccga cacaatgcaa tctgaagtgt tcggtacctg
      721 ggcgagggac gagaatccgc caaaggggtg ggggaaccgg cagagcgggc ggccgccctg
      781 ccgcggctcg cggcagtacg gtcggagcgg tcaaggagcg cgggcctttc ggatgctccg
      841 ggcgacggca tgggcggcta gagcgggaca aggtagccga cagacatgcg gacatcgtgg
      901 aggcggacgg gttgggagaa agtgcggagg cggctgtcgc cggtgtcgcc ggtgatgcgg
      961 atggtggggc gcgcctccga catgccgatt tcgagcgaga agttgcgatt gaagcgaagg
     1021 ccgagggtcg cccgatagcc gattttcggg gccatggcga tgtcgaggcc gacggcgccg
     1081 ggaacgaaat cggtcggttc gaggccgtcg aaacggaagt atcccttgta gaggaggggg
     1141 cccatgccga caaacccggt catggcatcg gtgccccggt agaggaggat ctcggcgctg
     1201 agaatcatag agtgcagcgc accgtcgggg taggtctttg agcgcatggc ggcgaatccg
     1261 taatcggcgc aggcgcgcag gaggaacggg tggcggagga ggaattcgta cttggcggag
     1321 atgaggagac cgggatcggt gaggacgttg ggtccgccga caccgatcgt gaaatgcatg
     1381 ttgacgccgt tcaccgtgtc ctcaacgacc gggttgagcg ggtagagcgg ggaaaccagc
     1441 caggcggcaa tcaggatggt cagcatactc gtagggcctc acagcggtga tctaaaagaa
     1501 taacgcaccg cgatcagaaa aagtcgaaga aaagttgcgg ggatggggc
//
LOCUS       DAOOKS010000530         1540 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_106023_length_1540_cov_3.292929, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000530 DAOOKS010000000
VERSION     DAOOKS010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106023_length_1540_cov_3.292929"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="PK112_08185"
     CDS             <1..512
                     /locus_tag="PK112_08185"
                     /inference="COORDINATES: protein motif:HMM:NF023385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3473 domain-containing protein"
                     /protein_id="HPC12062.1"
                     /translation="APSWSINDSIPWAFSILAELEFDYDSSIYPIKHDIYGMPHGPRV
                     IHRMHLEDDRLLYEVPASTYRLLGSNIPIGGGGFLRHSPYWYSKHIIRRLNTSGVPAV
                     VYMHPWEIDPDPPKLAGLTAMQRLRMYGSTSIFYHKLDRLLSDFEFCALADYLRLLKR
                     RQIGFLERP"
     gene            621..1307
                     /locus_tag="PK112_08190"
     CDS             621..1307
                     /locus_tag="PK112_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12063.1"
                     /translation="MLKRLLLAVLALAVCVIPAEGAKKKPKAGAIKDGWYVDAKYDFK
                     FQVPGNWSVAIKDGDDPVRAVLVQKNYQIPPEYLDAEDYTEVPRLTVYVLQTTMSALE
                     FRDSLLSKTYESDQKKDIRKYFEILNDVSIGEGTRREPVVTKSRELLYLDSLPASLWF
                     GEAQYVKNVTLSAAAQSGKRVNGDYAGTIVAVKNGDNLLLFHLMSEGMYHSSVWQEIR
                     TMIEGLKWPR"
BASE COUNT          314 a          437 c          484 g          305 t
ORIGIN      
        1 gggcgccgag ctggtcgatc aacgacagca tcccgtgggc gttcagcatc ctggcggaac
       61 tggaattcga ctacgacagc tcgatttacc ccatcaagca cgacatttac gggatgccgc
      121 acgggccgcg ggtgatccac cgtatgcacc tggaggacga ccggctcctg tatgaagtgc
      181 cggcctcgac ctaccgcctt ctcggatcaa acatccccat cggcggcggc gggtttctcc
      241 ggcattcccc ctactggtac tcgaaacaca tcatccggcg gctgaacacc tcgggagtgc
      301 cggcggtcgt ctacatgcac ccctgggaga tcgaccccga cccccccaaa ctggccgggt
      361 tgacggcgat gcagcggctg cgcatgtacg gctcgacctc gattttctac cacaagctcg
      421 atcgcctgct cagcgatttc gagttctgcg ctctggccga ttacctgcgc cttctgaagc
      481 ggcggcagat cggatttctc gaacgcccgt agccggcggg ggcgggagcg cgacgttttc
      541 cgcaaaatat ccttgactaa atgtgtataa gagtaaacct tggttgggag accggagaac
      601 caagaggagg ttaatgacaa atgcttaaac gactgctact tgcggttctt gctctggctg
      661 tttgcgtgat cccggcggaa ggcgccaaga agaagccgaa agcgggggcg atcaaggacg
      721 gctggtatgt cgacgcgaaa tatgacttca aattccaggt gccgggaaac tggagcgtgg
      781 cgatcaagga cggggacgac ccggtgcggg cggtcctcgt gcagaaaaac taccagatcc
      841 ctccggagta cctcgatgcc gaggactaca ctgaagtgcc tcgcctgacg gtctatgttc
      901 tgcagacgac gatgagcgcc ctggagttcc gcgattcgct gttgtcgaag acctacgaat
      961 cggaccagaa gaaggatatc cggaagtact ttgagatcct caatgacgtg tcgatcggcg
     1021 aggggacgcg gcgggagccg gtggtgacca agtcgcgcga gctcttgtat ctcgactccc
     1081 taccggcgtc gctctggttc ggtgaggcgc agtacgtgaa gaacgtcacc ctctctgcgg
     1141 ccgcgcagag cggcaagcgg gtgaacggcg attacgccgg gaccatcgtg gccgtcaaaa
     1201 acggcgacaa cctgctgctg ttccacctga tgagcgaagg tatgtaccac tcctcggtgt
     1261 ggcaggagat tcggaccatg atcgaggggc tgaagtggcc gcgctgagcg ggcagcgtgc
     1321 tacagtttgt tcgggaaagg ccggcgggga ccggcctttc ttttatgttg accgggcgct
     1381 tccgcagtcc tatcatgtaa agctgaggtt tcagcgagcg atggaggtcg tatgtgggcc
     1441 gtcagggccg tgctcatcac cgtgatcgtc atcgcggtcg tcgcgtttgc gatccataac
     1501 gtcggcgaat accagaaggt cgacgtcaac ctgatttgga
//
LOCUS       DAOOKS010000531         1539 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_106122_length_1539_cov_3.619946, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000531 DAOOKS010000000
VERSION     DAOOKS010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106122_length_1539_cov_3.619946"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1539
                     /locus_tag="PK112_08195"
     CDS             <1..>1539
                     /locus_tag="PK112_08195"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HPC12064.1"
                     /translation="YLVLVDARALPALAESGLRYDLIATTVDRSTLALGTHHDGANPG
                     GYPTVYAEDGVRLYRVEPGDVRDRAEADGLASLPPGSRRIVYREPAPREVKQRAPGSL
                     DLETLIALVRQDSLQSYTLQLQAFPNRVTGSLGNRQSRDWLQNRLISYGLDSAYQDSF
                     LYGSTNCQNVIGVKMGTTLPDHRIVVGAHRDAVSGSPGADDNGSGTAAVLEMARVMAD
                     IPTEMTWVFALFDSEEQGLNGSYHYAASAAANGDSIIMMLNMDMIGYNGNVNNVSVHY
                     GEDTVWASRWKIIADSLSTNLIGYFSGSASNSDHYPFQQEGYDVVFLIEYNFSTVYHS
                     EQDSTTYMSFPYMRRIVQASLATAYDADGAYLPTPGVLFTEVDPIPFMVPPVDTSEFL
                     ISITPGPGGAIAPGSAQVHFRYDAGAWQTGPLADLGGGTYAVPLPGPLCGERVQFYVS
                     AAEVTTGMYTYPDSASPWSAAAATQFTTALADNFETAQGWTVTGNATAGQWARGVPAG
                     GGSRG"
BASE COUNT          259 a          499 c          543 g          238 t
ORIGIN      
        1 tacctcgtcc tggtcgatgc ccgggctctc ccggcgctcg ccgagagcgg gctgcgctac
       61 gacctgatcg cgaccactgt cgaccgcagc acgctggcgc tcggcacgca ccacgacggc
      121 gccaacccgg gcggatatcc gacggtgtat gccgaggacg gcgtgcgcct ctaccgggtg
      181 gagccgggag acgtgcggga cagggcggag gcggacggtc tcgcgtcgtt gcccccaggg
      241 agccggcgaa tcgtgtaccg cgagccggcg cctcgggagg tgaagcagcg cgcgccgggg
      301 agcctggacc tcgagacgct gatcgccctg gtgcggcagg actcgctgca gtcgtacaca
      361 ctccagctcc aggccttccc caaccgggtc accggttccc tggggaaccg gcagtcgcgc
      421 gactggctcc agaaccgcct catcagctac ggccttgact ctgcgtacca ggacagcttt
      481 ctctacggct cgacaaactg ccagaacgtc atcggcgtca agatggggac gaccctcccc
      541 gaccaccgca ttgtggtcgg ggcgcaccgg gacgcggtgt cgggctcgcc gggagcggac
      601 gacaacggct ccgggacggc ggccgtgctc gagatggcgc gggtgatggc ggacataccg
      661 acggagatga cgtgggtgtt cgcgctgttc gactccgagg agcaggggct gaacggatcc
      721 taccactacg cggcctcggc ggcggccaac ggcgacagca ttatcatgat gctgaacatg
      781 gatatgatcg gatacaacgg aaacgtgaac aacgtgagcg tgcactacgg cgaggacacg
      841 gtgtgggcct cgcgctggaa gatcatcgct gactcgctgt cgacgaacct gatcgggtac
      901 ttcagcggat cggcctcgaa ctccgaccac tacccgttcc agcaggaagg gtatgacgtc
      961 gtcttcctca tcgagtacaa cttctcgacc gtgtaccact cggagcagga cagcacgacc
     1021 tatatgagtt ttccgtacat gcggcggatc gtgcaggcgt cgctggcgac cgcgtacgac
     1081 gcggacggcg cgtacctgcc gacgccgggg gtgctcttca cggaagtcga cccgatccca
     1141 ttcatggtgc cgccggtgga cacgtccgag tttctgatct ccatcacgcc cggtccgggc
     1201 ggggcgattg cccccggcag cgcgcaggtg cacttccggt acgacgccgg ggcgtggcag
     1261 accggaccgc tggccgatct cggcggcggc acgtacgcgg tcccgctgcc tggaccgctc
     1321 tgcggggaaa gggtgcagtt ctatgtgagc gcggccgagg taacgaccgg gatgtacacc
     1381 tatccggact cggcctcgcc gtggtcggcg gcggcggcga cgcaattcac caccgcgctg
     1441 gccgacaatt ttgaaacggc gcaggggtgg acggtgacgg ggaacgcgac cgccggtcag
     1501 tgggcgcggg gcgtgccggc gggcggcgga tcgcgcggt
//
LOCUS       DAOOKS010000532         1538 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_106247_length_1538_cov_2.861767, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000532 DAOOKS010000000
VERSION     DAOOKS010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106247_length_1538_cov_2.861767"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(220..307)
                     /locus_tag="PK112_08200"
     tRNA            complement(220..307)
                     /locus_tag="PK112_08200"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(271..273),aa:Ser,seq:gga)
     gene            complement(556..1335)
                     /gene="fetB"
                     /locus_tag="PK112_08205"
     CDS             complement(556..1335)
                     /gene="fetB"
                     /locus_tag="PK112_08205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HPC12065.1"
                     /translation="MSGANGPGFVQVALALVLVAIAVIVSRYWKLNLAKEMTLGTIRS
                     FVQLIAVGYALEFIFGLNTWWLIVLTILVMLTTGAYTSGGRIRGVDGALAIAWVSIAA
                     GSLVTIAVMVAVDIIPFTARYVIPLGGMIISNAMNAAALAMTRLISDLRAHTLAIETA
                     LALGKPWREASRGLQRDAATAGMLSTLNFMKTVGIVALPGAMTGMILGGADPLEAVLL
                     QIIVAYMLLSAVTITSIVAVDLTVRKFFTPHHQLRRLPALK"
     gene            complement(1332..>1538)
                     /locus_tag="PK112_08210"
     CDS             complement(1332..>1538)
                     /locus_tag="PK112_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HPC12066.1"
                     /translation="IERTVMDIVRRTGLTVIWVTHHPDQALRLGGETLLLVQGRLVES
                     GPSDEVINRPQTDLGRRYRARELR"
BASE COUNT          249 a          516 c          528 g          245 t
ORIGIN      
        1 tgacggatgc cggccgcgtg cgtgtaccgc ggtcctcttc aaaccgatag tcgctgatcg
       61 gccggcccct ccgtcccgcg gatccgcgac tacttccggc gacacggccg cccccggtct
      121 cgccttgcgc ccggaccgcc tcgcgggtgt aatccttgat ctcgtggtcc gacccgaccg
      181 ggtggttgtc gacagacaaa ccggggagac ctcgacaaat ggcggagagg cagggattcg
      241 aaccctggat aggtttccct atacacgcgt tccaggcgtg cgccttcagc cgctcggcca
      301 cctctccgtc gcacaacgag cgcccaccgc cggctgcacc agccggggcg gggctgccgt
      361 aaggtagggg aatcgctcca aaagacaagc tttttcggcc cccgcgcgcg tgctcgctcc
      421 gtggtagtga ctgaagtccc cgcccccgcg tattctcagc cccggcgcgc gggtcggctg
      481 cgcttccacg aagaacctgc gggcaggttt tccctgccgc gctcctcgat cggcggcggc
      541 ccgcggcgcc cggcctcact tgagcgccgg aagccggcgc aactggtggt ggggggtgaa
      601 gaacttgcgt acggtcaggt cgaccgccac gatcgacgtg atcgtcaccg ccgacagcag
      661 catgtaggcg actataatct ggaggagcac ggcctcgagc gggtcggcgc cgccgaggat
      721 catcccggtc atcgcccccg gcagggcgac gatccccacg gtcttcataa aattcagcgt
      781 ggagagcatg ccggcggtgg ccgcgtcccg ctgcagtccc cgcgaggcct cccgccacgg
      841 cttgccgagc gcgagggcgg tttcgatggc gagggtgtgg gcgcgcagat cggagatcag
      901 ccgggtcatg gcgagggccg cggcgttcat ggcattactg atgatcatgc cgccgagcgg
      961 gatgacgtag cgcgccgtga acgggatgat gtcgacggcc accatgacgg cgatggtgac
     1021 cagggagccg gcggcgatcg agacccaggc gatcgcgagc gcgccgtcga ctcccctgat
     1081 ccgtccgccc gaggtgtacg cgccggtggt cagcatgacg aggatggtga ggacgatcag
     1141 ccaccaggtg ttgaggccga agatgaactc cagggcataa ccgacggcga tgagctgcac
     1201 gaacgagcgg atcgtgccga gggtcatttc tttggccagg ttcagcttcc agtagcggga
     1261 gacgatgacg gcgatggcga ccagcacgag cgccagcgcc acctgcacga atcccggtcc
     1321 gttggccccg ctcatcgcag ttccctcgcg cggtagcgcc ggcccaggtc ggtttgcggc
     1381 cggttgatca cctcgtcgct cggtccggac tccaccaacc gtccctggac cagcaggagc
     1441 gtctcgccgc caagccggag ggcctggtcc gggtggtggg tgacccagat aacggtcagg
     1501 ccggtgcggc gcacgatgtc catgaccgtg cgctcgat
//
LOCUS       DAOOKS010000533         1537 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_106336_length_1537_cov_3.114710, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000533 DAOOKS010000000
VERSION     DAOOKS010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106336_length_1537_cov_3.114710"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="PK112_08215"
     CDS             <1..603
                     /locus_tag="PK112_08215"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HPC12067.1"
                     /translation="YHRAERDERLMSRGVDFLFHAVIDAVVDNYNATLDILEYEIDKI
                     EDDVLEDLDQETLRSIFTLRRDIIQLRRIVIPQKEVVLHLSREQFPQISTRAAVYLSD
                     IHDHLVRIDDLANFHREILNSSLEIYYSSISTRTNEAIKVLTILSVLFIPPTFLVGVW
                     GMNFRNMPELEWRYGYGAALLFIFLIIGGLLVYFRKKKWI"
     gene            679..1317
                     /locus_tag="PK112_08220"
     CDS             679..1317
                     /locus_tag="PK112_08220"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPC12068.1"
                     /translation="MANRQVKKRIRQSPKRPAGERRDQLLRAAHELFLRQGYRATSTD
                     EIARRAGLTKGAFYFHFASKEDILMTFVQDFVEGHTRAMERLAGKRLTPAGLLAELGR
                     IDARMPLREARKNLSLLAEIMYLPRFRALIRRAYERSVAVMAECLDPGYGRTAAQRRR
                     LVELMHAIYDGVCFAGMVHPQLSNFERQLETFRRLTAKKRIRSNRKHGPAKS"
     gene            1298..>1537
                     /locus_tag="PK112_08225"
     CDS             1298..>1537
                     /locus_tag="PK112_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12069.1"
                     /translation="MVPRKVEKPGRAAGPVRRWGGVVALAAALAAAGAAAAADGARPD
                     TMVLTAAEVRRLALEYNRDYLQALEEVRAADAEVVK"
BASE COUNT          272 a          459 c          541 g          265 t
ORIGIN      
        1 taccaccggg cggagcggga cgagcggctg atgtcgcggg gggtggattt cctgttccac
       61 gcggtgatcg acgcggtggt cgacaactac aacgcgaccc tcgacatcct cgagtacgag
      121 atcgacaaga tcgaggacga tgtgctggag gatctcgacc aggagacgct ccggtcgatt
      181 ttcaccctcc gccgcgacat catccagctc cgccggatcg tgatcccgca gaaggaggtg
      241 gtgctccacc tgtcgcggga gcagttcccg cagatttcga cgcgggcggc ggtgtacctg
      301 tcggacattc acgaccacct cgtgcgaatc gacgacctgg cgaatttcca ccgcgagatc
      361 ctcaactcgt cgctcgaaat ctactactcc tcgatttcga cgcgcaccaa cgaggcgatc
      421 aaggtcctga ccatcctcag cgtgctgttc attccgccga ccttcctcgt gggcgtgtgg
      481 gggatgaatt tccggaacat gcccgagctc gagtggcgct acgggtacgg ggcggcgctc
      541 ctgttcattt tcctgatcat cggcgggctg ctcgtctatt tccgcaagaa aaagtggatt
      601 tagcggaaaa ttgtttgaaa cttcccttga caacgggccg ttcatcccct atagataccg
      661 attggtaggt atgtagagat ggcgaaccga caggtgaaaa agcgcatccg ccagagcccg
      721 aagcggccgg ccggcgagcg ccgggaccag ttgctccggg cggcccacga gctgttcctc
      781 cggcagggct accgggcgac gagcacggac gagatcgccc gccgggcggg cctcacgaag
      841 ggggcgttct acttccactt cgccagcaag gaagacatcc tcatgacttt cgtccaggac
      901 ttcgtcgagg ggcacacgcg ggcgatggag cgcctggcgg ggaagcggct gacgccggcg
      961 ggtcttttgg ccgagctggg gcggatcgac gcgcgcatgc cgctgcggga ggcgcgcaag
     1021 aatctctcgc tgttggcgga aatcatgtac ctgccgcgct ttcgcgcgct gatccgccgg
     1081 gcttacgagc ggtcggtggc ggtcatggcg gagtgtctcg acccgggcta cgggcggacg
     1141 gccgcccagc ggcggcggct ggtcgagctc atgcacgcga tctacgacgg cgtgtgtttc
     1201 gccggcatgg tgcatccgca gctcagcaat ttcgaacgac aactggaaac attcaggcgg
     1261 ctgacggcca agaagaggat acggagtaat cgaaagcatg gtcccgcgaa aagttgagaa
     1321 accgggccgg gcggccgggc cggtgcggcg gtggggcggg gtggtggcgc tggcggcggc
     1381 tctggcggcg gccggcgccg cggcggcggc cgatggggcg cgcccggaca cgatggtgct
     1441 gacggcggcg gaggtccgcc gcctggcgct tgagtacaac cgcgattacc tgcaggcgct
     1501 cgaggaggtg cgggcggccg atgccgaggt ggtgaag
//
LOCUS       DAOOKS010000534         1530 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_107066_length_1530_cov_3.437288, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000534 DAOOKS010000000
VERSION     DAOOKS010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107066_length_1530_cov_3.437288"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="PK112_08230"
     CDS             complement(<1..396)
                     /locus_tag="PK112_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12070.1"
                     /translation="MPAASPRKPHRRPPAASLRPFLLAYAFTVMAFFLLSFVPHGRVW
                     GLNSWAWLPLWGRLLLLIAAAAAPLGALRLSEPSAPAPGRRGAYALAAGLAGIVLFAA
                     FLILRQQTHFLGDGYTLLKWLEEGGAINKM"
     gene            complement(400..1383)
                     /locus_tag="PK112_08235"
     CDS             complement(400..1383)
                     /locus_tag="PK112_08235"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPC12071.1"
                     /translation="MKHLAALTVLFLAASAAAVEIKPQGVPVTSGDDITADSLLRQGD
                     AEFQARDYAAALRTYLSAVDQARREFNRSVETEALAQTARMCLVQDRKEEGRPYLEQA
                     AAIAVDSDPMGWSRYLGVRGRYQWQDSELAAARETFELMFDYCSVNALFGRAVDAAHM
                     VAIVAASPEDQIAWNRRGIAAAEAADEERWLGPLWNNLGGTYWDNGQFDSALACYLKA
                     RDYHWRFSDETAKLFADYHVGMTYRHLGDYREAQQWLRPVLAWAERLDNREAIGQACE
                     DLGEALLAEGKRAEGTALLRRARDAYRAAGYDRTRPEIWDNITRRLKELGE"
BASE COUNT          241 a          495 c          565 g          229 t
ORIGIN      
        1 catcttgttg atggcgcccc cctcctcgag ccacttcagc agcgtgtagc cgtcgccgag
       61 aaagtgggtt tgctggcgga gaatcaggaa cgcggcgaag aggacgatcc cggcgaggcc
      121 ggcggcgagc gcgtatgcac cccgcctgcc cggcgcgggc gcagacggtt cggaaaggcg
      181 gagcgctccc agaggcgcgg cagcggccgc gatcagcagc agcaaccgtc cccacagcgg
      241 cagccaggcc cacgaattca atccccacac gcgcccgtgg ggcacgaacg agaggaggaa
      301 gaaggccatc acggtgaacg cgtaggcgag gagaaagggc cggagggagg cggcgggggg
      361 acggcggtgt ggtttgcgag gggaagccgc gggcacgaat tactccccca gctccttgag
      421 gcgcctggtg atgttgtccc agatttccgg ccgggtgcgg tcgtacccgg cggcgcggta
      481 ggcgtcgcgg gcgcggcgca aaagcgcggt tccttcggcg cgcttgccct cggcgagcag
      541 ggcctcgccg aggtcctcgc acgcctgtcc gatggcttcg cggttgtcaa ggcgttcggc
      601 ccaggcgagc accgggcgca gccactgctg cgcctcccgg tagtctccca ggtggcggta
      661 ggtcatgccg acgtggtagt cggcgaagag tttggcggtc tcatcggaga agcgccagtg
      721 gtagtcgcgg gctttgagat agcaggccag ggcgctgtcg aactgcccgt tgtcccagta
      781 ggtgccgccg aggttgttcc atagggggcc gagccagcgc tcttcatcgg ccgcctcggc
      841 ggcggcgatc ccccgccggt tccaggcgat ctggtcctcg ggcgacgcgg cgacgatcgc
      901 gaccatgtgg gcggcgtcca cggcccggcc gaacagggcg ttgaccgagc agtagtcaaa
      961 catgagttcg aatgtttcgc gggcggccgc gagctcggaa tcctgccact ggtaccgccc
     1021 ccggacgccg aggtaacgcg accagcccat ggggtcggaa tccacggcga tggcggcagc
     1081 ctgttcgagg tagggccgcc cctcctcttt ccggtcctgc acgaggcaca tgcgggccgt
     1141 ctgggcgaga gcctcggtct cgacggagcg gttgaactcc cggcgggcct ggtcgacggc
     1201 cgacaggtag gtgcgcaggg cggccgcata gtcgcgggcc tgaaactcgg cgtccccctg
     1261 gcggagcagg gagtcggcag tgatgtcatc gccagaggtc acgggtactc cttgtggttt
     1321 gatctcgaca gcggcggcgg aggccgccag gaacagcacg gtcagtgcgg cgaggtgttt
     1381 catgccgtct cctcggtttg catccggtgg cggacggaat ataatcgacc ggagcgggcg
     1441 ccgccaccgc aaatcgggcg ccgggcattt ccatttgacg cggccggacc gccccccgcc
     1501 acgcccaccg agcacatcct cgccgcggcc
//
LOCUS       DAOOKS010000535         1528 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_107259_length_1528_cov_3.994569, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000535 DAOOKS010000000
VERSION     DAOOKS010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107259_length_1528_cov_3.994569"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..970
                     /locus_tag="PK112_08240"
     CDS             <1..970
                     /locus_tag="PK112_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12072.1"
                     /translation="RVFPGRSGERKLERSGSGYEVGYLMPELRDTRQKAANRTLTVMV
                     PQDNPLIVTSPFGSVVLESLENDVMLTTEHADVTGSDIVGRVGLTCQMGSVRLTGVEG
                     HIAITARRTPVELQGCDGEVTIDNEYASVQLAECAGPVDIRNSGQVRISYHQGDISVD
                     NSNGQVEVQHSEGNCRLTSRYQPMQIAYVKGSVTAENSHAPIVISDITGRIEAGNSFA
                     PIQATDFSGPISLTNSNGPITVSVSGPLQGPSAIRSTGGVINLTLEPGIDLLMRATAV
                     DGKIESSYPLSLKQAGTTTVAELALGKARPELTITGTRTRIVVGED"
     gene            complement(1043..>1528)
                     /locus_tag="PK112_08245"
     CDS             complement(1043..>1528)
                     /locus_tag="PK112_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12073.1"
                     /translation="MQEAAFLEIEIGSNEVLLTEANRILYHQFIALERLLRENRAMQR
                     EIARGQLEKSALETLKTITATFNHYINNAVGAILGRAQLVQHDIRRGTFARNPESALE
                     SMEIIVNGVKTIRSVMEELTNLSAFKTTVYYDDTYIIDIEKKIRKKVEAIGDSPAPAA
                     K"
BASE COUNT          283 a          447 c          519 g          279 t
ORIGIN      
        1 gcgtgtgttt cccggccgca gcggggaacg gaagctcgaa cggtccggca gcggctacga
       61 ggtcggctac ctgatgcccg agttgcgcga tacccgccag aaggccgcga accggactct
      121 caccgtcatg gtgccgcagg acaacccgct catcgtgaca agtccgttcg gcagcgttgt
      181 gctcgaaagc ctggaaaacg atgtgatgct caccaccgaa cacgccgacg tgaccggcag
      241 cgacatcgtg gggcgggtag ggctgacgtg ccagatggga tcggtgcgtc tgaccggagt
      301 cgaggggcat attgcgatca ccgcgcgccg tacgccggtg gagctgcagg gctgcgatgg
      361 ggaggtgacg attgacaacg agtacgcatc ggtgcagctg gccgagtgcg ccgggcccgt
      421 cgacatccgc aacagcggcc aggtgcgcat cagctaccac caaggggata tcagcgtcga
      481 caactccaac ggccaggtgg aggtccagca ctccgaggga aactgccggc tgaccagccg
      541 gtaccagccg atgcagatcg cgtacgtgaa ggggtcggtg acggccgaga acagccacgc
      601 cccgattgtg atctccgaca tcaccgggcg gatcgaggcc ggcaactcct tcgctcccat
      661 ccaggccacc gacttctcgg gaccgatcag cctgactaat tccaacggac caattacggt
      721 gagcgtgtcg ggaccgctgc agggaccatc ggcgatccgc tcaaccggcg gcgtgatcaa
      781 cctgacgctg gaaccgggca tcgacctgct catgcgggcg actgcggtcg acggcaagat
      841 cgagtcatcc taccccctgt cgctgaagca ggccggaacg acgactgtgg cggagctggc
      901 gctggggaaa gcgcggccgg aactgacgat tacgggaacg cggaccagga tcgtggtggg
      961 ggaagactga gcccgcgggg cgacccttca gagcttctct ccgactttcg ataaatcatc
     1021 taatccattg gtgtccaacc gcttacttcg cggccggggc cgggctgtcg ccgatcgctt
     1081 cgactttttt ccggatcttc ttttcgatgt cgatgatgta cgtgtcgtcg tagtaaacgg
     1141 tggtcttgaa ggcggagaga ttggtgagct cctccatcac tgagcggatc gtcttcacgc
     1201 cgttgacgat gatctccatc gattcgagcg cgctctcggg attccgggcg aacgtgcccc
     1261 gacggatatc gtgctggacg agctgggcgc ggccgaggat agcgccgacc gcgttgttga
     1321 tgtagtgatt gaaggtggcg gtgatggttt tgagggtctc gagggcggat ttttcgagct
     1381 ggccgcgggc gatctcgcgc tgcatggcgc ggttctcgcg cagcagccgc tcgagcgcga
     1441 tgaactggtg gtagaggatg cggttggcct ccgtgaggag cacctcgttg gagccgattt
     1501 cgatttcgag gaaggccgcc tcctgcat
//
LOCUS       DAOOKS010000536         1528 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_107264_length_1528_cov_3.391039, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000536 DAOOKS010000000
VERSION     DAOOKS010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107264_length_1528_cov_3.391039"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="PK112_08250"
     CDS             <1..456
                     /locus_tag="PK112_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPC12074.1"
                     /translation="RTNFLGRWISHCGWYPDPVLRLFRREKGNVNEAVVHEQVEVDGS
                     VGRLSGELLHFSYPTLEHYLAKSAVYTTLGAREAFRRGRRARWYDLVIRPPISFFSHY
                     IVRQGFRDGMEGWIISVMSAVAVFVKYAKLRHLQRTATPEQREQYGFGD"
     gene            440..1483
                     /locus_tag="PK112_08255"
     CDS             440..1483
                     /locus_tag="PK112_08255"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HPC12075.1"
                     /translation="MALEIDLQPGDKILISRTDRLGDLILALPFVETIKRRYPECRVD
                     VLASLYASPILENNPHIDRIVRVQNDQLWTDNLYKKDLLHRIRMEGYRAVVALYPERQ
                     ISQLFQRAEIPIRIGTARRFHSIYFTHHVTHSRKANVKHEYEYNLDFLQFFRDGETVA
                     SPKVYLRERELRNARRILDDVGAEESFVVIHPGSRGSAERWPPERFIELYCQLSREGL
                     SVVLSGSEEEGQGLSDLTAGLPVAVRDISGQTDLRTLAAVLSQARVVVANSTGPLHLA
                     TAVGTHVVGLYPSRKAVSPRRWGPLGEQHRVIQPTGVKCECPPKGCRCMDTISVERVV
                     EEVVALYHVQSVG"
BASE COUNT          265 a          491 c          499 g          273 t
ORIGIN      
        1 cgcaccaatt tcctcggccg ctggatctcg cactgcggat ggtaccccga tccggttctg
       61 cgcctgtttc gccgcgaaaa ggggaatgtc aacgaggccg tggtccacga acaggtggaa
      121 gtcgacggat cggtggggcg gctgtccggc gaactcctgc acttcagcta cccgactctg
      181 gagcactacc tggccaaatc ggccgtttac acgactctcg gagcgcgcga ggcgtttcgc
      241 cggggccgcc gcgcgcgctg gtatgatctt gtcattcggc ccccgatctc gttcttttcg
      301 cattatattg tccggcaggg attccgcgat ggaatggagg gatggatcat ttcggtgatg
      361 tcggcggttg cggtctttgt gaaatatgcc aagctgcgcc acctgcagcg gaccgcgacc
      421 ccggaacaga gggagcagta tggctttgga gattgacctg cagccggggg acaaaatcct
      481 gatcagccgg accgaccggc tgggcgacct catcctcgcg ctgccctttg tcgagaccat
      541 caagcggcgc taccccgagt gccgcgtcga cgtcctggcg tcgctgtacg cctcgccgat
      601 tctggagaat aacccgcaca tcgaccggat cgtccgggtg cagaacgacc agctctggac
      661 cgacaacctg tacaagaagg acctcctgca ccggatacgg atggaaggct accgggcggt
      721 cgtggcgctg taccctgaac ggcagatctc gcagttgttc cagcgggccg aaatcccgat
      781 ccgcatcggg accgcccgcc ggttccactc gatctacttc acccaccacg tcacccacag
      841 ccgcaaggcc aacgtgaaac acgagtacga gtacaacctc gacttcctcc agttcttccg
      901 cgatggcgag acggtggcca gcccgaaggt gtacctgcgg gaacgggaac tgcgcaacgc
      961 gcggcgcatt ctcgacgacg tcggggcgga ggaatcgttt gtcgttatcc accccgggtc
     1021 gcgcgggtcg gccgaacgct ggccgcccga gcgctttatc gagctctact gccagctgtc
     1081 ccgggagggg ctgagcgtcg tgctctccgg atcggaggag gagggccagg ggctgtcgga
     1141 tctcaccgcc gggctgccgg tggccgtgcg ggacatctcc ggccagaccg atctgcggac
     1201 gctggcggcc gtgctgtcgc aggcgcgggt ggtggtggcc aactccaccg gcccgctcca
     1261 cttggccacc gccgtcggca cccacgtcgt gggcctctac ccgagccgca aggcggtctc
     1321 gccgcggcgc tggggaccgc tcggcgaaca gcaccgggtc atccagccga ccggagtcaa
     1381 gtgcgagtgc ccccccaagg ggtgccggtg catggatacc atctcggtgg agagggtggt
     1441 cgaggaagtc gtggccctgt accacgtgca gtcagtcgga tgacggcgat atgaatctgg
     1501 ccgagttcat catacggatc gaatcgtt
//
LOCUS       DAOOKS010000537         1528 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_107268_length_1528_cov_3.190767, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000537 DAOOKS010000000
VERSION     DAOOKS010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107268_length_1528_cov_3.190767"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="PK112_08260"
     CDS             <1..268
                     /locus_tag="PK112_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007789222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HPC12076.1"
                     /translation="TTVSEEYELEYGTDRLEVHADAVLPGDRVVVVDDLIATGGTLLA
                     ACRLVERLGGVVAGVAAVIDLRFLPWREKLSGYDVRYLVSYDSE"
     gene            336..412
                     /locus_tag="PK112_08265"
     tRNA            336..412
                     /locus_tag="PK112_08265"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:370..372,aa:Arg,seq:cct)
     gene            612..>1528
                     /locus_tag="PK112_08270"
     CDS             612..>1528
                     /locus_tag="PK112_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013061480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12077.1"
                     /translation="MKSLTVKGTVVLLLIGTTVFAQGGVGLEYGNRLEFDVWDFSRPA
                     LEGYQIDFSGGGARALGMGRAFLGLSDDVTAISWNPAGLYTLSQPVISATYGSTLPRG
                     YTDTDPQFVLDPSQTRFDHNGWISGITALDFAAPLRIKGHPFVGSFTYSTGFDEYQGY
                     QFSFDTTFRDTFTTPDTIFVDTVVHYTVNRESEMDGALKAVSFGFGTRIYREWSVGAA
                     LNVYSGTAVRHAASYESSQDRIDYSFQEYSQVYATTVIDSNRFSGYNFTLGVRYNASP
                     LAAGLIIRTPFDLEVETDRSIYEIAKKNER"
BASE COUNT          280 a          433 c          530 g          285 t
ORIGIN      
        1 tacgacggtg agcgaggagt acgaactgga gtacgggacc gaccggctcg aggtgcatgc
       61 ggatgcggtc ctgccgggtg accgagtggt ggtggtcgat gatttgatcg ccaccggggg
      121 aacgctcctg gccgcctgcc ggctggtcga acggctgggc ggcgtcgtgg ccggggtggc
      181 ggcggtgatc gatctgcgct tcctcccgtg gcgggagaaa ctctccgggt acgatgtccg
      241 ctacctggtc tcctatgact cggagtgagg gggaaaaggt cgttgacaaa gccgggcggc
      301 gagggtttct tgcggggcgg attgcgcgag acggcgcccc cgtagctcag tcggatagag
      361 cagcggtttc ctaaaccgcg tgccgcaggt tcgattcctg ccgggggtac caaaaaagac
      421 ggcaggaaag gggcaaggat tgcgccggag gaggtcaacc cattcgcgaa catgcatcta
      481 gaccggggac ccggcgggga aatcgtttga caggcgattg gggcaggcct atatttattg
      541 gttaattttc ggtcccggtg cgtctgtagc agtggaagtt agttgtgcga tacgataagg
      601 agtcgaaggt aatgaagtcg ttgacggtga agggcacggt ggtgctgctg ctgatcggaa
      661 caacggtgtt cgcccagggg ggcgtgggcc tggagtacgg aaatcgtctg gagttcgacg
      721 tctgggattt cagccggccc gctctcgagg gctaccagat cgatttcagc ggcggcggcg
      781 cgcgcgcgct ggggatgggg agagcgttcc tcgggctgtc ggatgacgtg accgcgatca
      841 gctggaaccc ggcgggattg tacactctca gccagcccgt catctcggcc acctacggga
      901 gcaccctgcc gcgcgggtac accgacaccg acccgcagtt cgtgctcgat ccgtcgcaga
      961 cgcgcttcga ccacaacggg tggatcagcg ggatcacggc cctggatttc gcggcccccc
     1021 tccgcatcaa gggacacccc ttcgtcgggt cgttcaccta ctcgacggga ttcgacgagt
     1081 accaggggta ccagttctcg ttcgacacca cgttccgcga caccttcacc accccggaca
     1141 cgatattcgt ggacaccgtg gtgcactata cggtgaaccg ggagtcggag atggacgggg
     1201 cgctgaaggc ggtgagtttc ggtttcggga cgcggattta ccgcgagtgg tcggtcggcg
     1261 cggccctcaa cgtgtacagc ggaacggcgg tgcggcacgc ggcgagttac gaatcctccc
     1321 aggatcgcat cgactactcg ttccaggaat actcgcaggt gtatgcgacg accgtgatcg
     1381 actccaaccg gttttcgggc tacaacttca ctctcggtgt gcgctacaac gccagcccgc
     1441 tcgcggccgg gcttatcatc cggaccccct tcgacctcga agtggagact gaccggtcga
     1501 tctacgagat cgccaagaag aacgagcg
//
LOCUS       DAOOKS010000538         1527 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_107354_length_1527_cov_4.156929, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000538 DAOOKS010000000
VERSION     DAOOKS010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107354_length_1527_cov_4.156929"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="PK112_08275"
     CDS             complement(<1..192)
                     /locus_tag="PK112_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12078.1"
                     /translation="MRRSFCLACAFLAAGAMGAGADNFQGSFDSPYTLPDSVVVTANR
                     FGSSDTRSAWPTEVITVDPA"
     regulatory      complement(256..443)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(493..1329)
                     /locus_tag="PK112_08280"
     CDS             complement(493..1329)
                     /locus_tag="PK112_08280"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pepsin/retropepsin-like aspartic protease family
                     protein"
                     /protein_id="HPC12079.1"
                     /translation="MRRLSLLFLAAAVPAAAAAASVVRFDPLQGLVEVEAVIDGHAKG
                     RFGIDTGADHFYIDSAFAAAHGLNLASGRPSRAVVGIDGHSTAIAGQVRSLEIGDERL
                     YNLDAIVMDIGALVKDDRADPPDGLIGYSLLRRFYVTVDYPNRRLILQQTEPDFLQSG
                     GLPSVPFETKGHLVVVQATFDDSATAPMALDYCASYVAVSPALAERLGVPAAAERTIL
                     GRLSLGGLITTDSVAVGIADISPIKARLRDAAFEGLIGASFLYRHKITIDYRRGRIYV
                     HR"
     gene            complement(1389..>1527)
                     /locus_tag="PK112_08285"
     CDS             complement(1389..>1527)
                     /locus_tag="PK112_08285"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HPC12080.1"
                     /translation="CSGLPRITAELAARGYSESDIRKILGGNFLRVFRRVCDRSPAAS
                     G"
BASE COUNT          274 a          496 c          541 g          216 t
ORIGIN      
        1 ggccggatca acggtgatca cctcggtcgg ccaggccgac cgcgtgtccg aggaaccgaa
       61 ccggttggcg gtcacgacca ccgaatcggg cagggtgtag ggggaatcga atgatccctg
      121 aaaattgtcg gccccggctc ccatcgcgcc ggcggccaga aacgcgcagg ctaaacaaaa
      181 actgcgccgc atggcaacct ccaccggttg cggccgcgca gtgcctcgat ctcgtcagcc
      241 cgcgctgaca gcaaccgcct gagatgtggt cgcggcaggt ttcctggctt cggcatccaa
      301 ccctcgccgg gcgccttccc cgcgaatccg cgagtggcct ggtgcccggg aagtagccgt
      361 tacagtagcg gggctgcgac ggactcgcac cgtcttccct attacccgtc cctctggacg
      421 gcaccgcaac gttgacgcga ctatcgtagc gacgggaggg gcggctgtca agcggaaagc
      481 ggagaggttg gcctaccggt gcacgtagat gcggccgcgc cggtaatcga ttgtgatctt
      541 gtggcggtag agaaaggacg ccccgatgag gccctcaaaa gcggcgtcgc gcaggcgggc
      601 tttgatcggg gagatatcgg cgatgccgac agcgacgctg tcggtggtga tgaggccgcc
      661 gagcgacagg cggccgagaa tcgtccgctc ggccgcagcg ggcaccccga gccgttcggc
      721 gagcgcgggg gagacggcca cgtacgaggc gcagtaatcc agcgccatcg gcgccgtcgc
      781 cgaatcgtcg aacgtcgcct ggaccaccac gaggtgcccc ttggtctcga acggcaccga
      841 gggaagtccg ccggactgga gaaaatccgg ctcggtctgc tggagaatga ggcggcggtt
      901 cgggtaatcg accgtgacgt agaagcggcg cagcaggctg tacccgatga ggccgtcagg
      961 aggatcggcc cggtcatcct tgaccagcgc cccgatgtcc atcacgatcg catcgaggtt
     1021 gtacaggcgt tcgtcgccga tctccagcga gcgcacctgg ccggcgatcg ccgtgctgtg
     1081 gccgtcgatc cccactaccg cgcggctggg gcggccggag gccaggttga gaccgtgcgc
     1141 ggcggcgaag gcgctgtcga tgtagaaatg atcggccccc gtatcgatgc cgaaccggcc
     1201 tttcgcatgg ccgtcgatca ccgcctccac ctcgaccagc ccctgcagcg gatcgaagcg
     1261 gaccacgctc gccgccgcgg ccgctgccgg caccgccgct gcgagaaaga gcaggctcag
     1321 gcgtctcata tccggaagaa acagggccgg ggcgggccgg ggcaagcgaa aacgccgggg
     1381 aggggggatc agccggacgc ggccggcgac cggtcgcaca cgcgccggaa gacgcgcagg
     1441 aaattgccgc cgaggatttt cctgatgtcg ctctccgagt agccccgggc ggcgagctcg
     1501 gccgtgatgc gcggaagccc ggagcag
//
LOCUS       DAOOKS010000539         1527 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_107410_length_1527_cov_2.550272, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000539 DAOOKS010000000
VERSION     DAOOKS010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107410_length_1527_cov_2.550272"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(199..1389)
                     /gene="sucC"
                     /locus_tag="PK112_08290"
     CDS             complement(199..1389)
                     /gene="sucC"
                     /locus_tag="PK112_08290"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF001913.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HPC12081.1"
                     /translation="MRLYEHEGKQLFEKIGIPTAPRRVCRTPEEARAAALELGPVVVK
                     AQVLTGGRGKAGGVKLAATPEEAQHIAADLLAVKIRGYEVAAVLVEPRLDIARELYLG
                     VTIDRANYTVVVIASGEGGVDIEETAVRNPEKIVRHHLRIDEEFYGFDALAIAKQIGL
                     PSDLLKSAAPIIVNLYRLFRRCDAKLVEINPLVLTADNKLIAADARVSLDDDALFRHP
                     ELQALGIAHRHEEGEMTPRERRAAEWGIPYLDLDGDIGMFPGGAGFGIMGNDFIHYYG
                     GRPANFMDSGGGPTPERLANMLVLLDENPNVKAIFAARFGGISRCDDFAKGVIKFLRE
                     HGLSKPMVMRMTGNMWQEGVRLFEEARTKSPELFARIEIHGIETPIEEISRRAVELAR
                     GTAS"
BASE COUNT          246 a          438 c          560 g          283 t
ORIGIN      
        1 agatgtcagg accacaaggg gcctgaccta caggaggaga cggagtccgg gagaccggag
       61 tgtggtggtg ctgccggaac gcaggtcggc caagagaatc cggccggcgt gtcggccttt
      121 gcccggccat cgatcctgct cctctcagcg ctgctcgcac gcgcaaggcg ccgcgcccgg
      181 ctgcggcctc tcccctgctc atgaggccgt ccccctggcc aattcgaccg cacggcggga
      241 gatttcctcg atgggagtct cgatgccgtg gatctcgatg cgcgcaaaaa gctcgggcga
      301 cttagtgcgg gcctcctcga acaggcggac gccctcctgc cacatgttgc cggtcatgcg
      361 catcaccatc ggtttggaga ggccgtgctc gcgcaggaat ttgatgaccc ctttcgcgaa
      421 atcatcgcag cgggagatgc cgccgaagcg cgcggcgaaa atggctttga cgttggggtt
      481 ttcatcgagc aggacgagca tgttggcgag gcgctcggga gtggggccgc cgccggagtc
      541 catgaagttg gcggggcggc cgccgtagta gtggatgaaa tcgttgccca tgatgccgaa
      601 accggcgccg ccggggaaca tgccgatgtc gccgtcgagg tcgaggtacg ggatgcccca
      661 ttcggccgcc cggcgctcgc gcggcgtcat ctccccctcc tcgtgccggt gggcgatgcc
      721 cagggcctgc agttcggggt gccggaacag ggcgtcgtca tcgagcgaca cgcgcgcgtc
      781 ggcggcgatg agtttgttgt cggcggtgag caccagcggg ttgatctcga cgagcttggc
      841 gtcacagcgg cggaagagcc ggtagagatt cacaatgatc ggagccgcgc ttttgaggag
      901 atccgagggg aggccgatct gtttggcaat cgcgagggca tcgaacccgt agaactcctc
      961 gtcgatgcgg aggtggtggc gcacgatttt ctccgggttt cggacggcgg tctcctcgat
     1021 atcgaccccc ccttcgccgg aagcgatgac gacgacggtg taattggccc ggtcgatggt
     1081 cactccgagg tacagctcac gcgcaatgtc cagccggggt tcgaccagaa cggcggcgac
     1141 ctcgtagccg cggattttca cggcgaggag gtcggcggcg atgtgctgcg cctcttcggg
     1201 ggtggcggcg agtttgacgc cgccggcttt gccccgcccg ccggtgagca cctgggcttt
     1261 gaccaccacc gggccgagtt ccagagcggc agcgcgcgcc tcctccggtg tacggcagac
     1321 gcggcggggg gcggtgggga taccgatttt ctcgaacagt tgtttacctt cgtgttcata
     1381 gaggcgcatg gtcagggtcc ttagtaaagg ggcgcgcagg ggccgatgtt cgcgttattt
     1441 gcgggataat gtagggagtc gggagcggga ggtcaagggg tgtcgatttc tgggttggga
     1501 aatggggcgg agggggttta ttgaggg
//
LOCUS       DAOOKS010000540         1527 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_107425_length_1527_cov_2.181386, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000540 DAOOKS010000000
VERSION     DAOOKS010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107425_length_1527_cov_2.181386"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="PK112_08295"
     CDS             <1..355
                     /locus_tag="PK112_08295"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HPC12082.1"
                     /translation="HLIAEVGAVVEILDKLDWRGVFEAYAGGNEVQAVKPDPEIFRLV
                     LARLGAVPRESLVIGDTINDILAAQAVPLPVIAVASPWEPAGRVLAACPDFFLDSISQ
                     LPRVLDEINTRRSPG"
     gene            complement(502..1161)
                     /locus_tag="PK112_08300"
     CDS             complement(502..1161)
                     /locus_tag="PK112_08300"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HPC12083.1"
                     /translation="MKLRIVQKHIAELDLVPWVEAADRAGMDLVCFGELAATGCLYEP
                     RPVEPVDRILQRLVPFGVGVMFGAVRVEVEGRYNSYFFRRHRQTWVYDKINLFPPFGE
                     DRIYRPGKVPGLFVTPFGDFGVAVCYDLRFDDIFARLAELAVPYLIVPAAFPQVRIED
                     WRRLLAQRARELGATVIGVNAVGSDGVNRFGGSSMVVAPDGSIVAQADETGEQILDVT
                     L"
     gene            complement(1330..>1527)
                     /locus_tag="PK112_08305"
     CDS             complement(1330..>1527)
                     /locus_tag="PK112_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12084.1"
                     /translation="VFFNGRRAASADVAPPRRPQGERLVARSSAVGRCPADWKWSSYN
                     SCLGRHYVPVRVDMVNMTVSE"
BASE COUNT          283 a          494 c          520 g          230 t
ORIGIN      
        1 tcacctcatt gcagaggtcg gcgctgtcgt cgagatcctc gacaagctcg actggcgggg
       61 ggtgttcgag gcctacgcgg gcggcaatga agtgcaggcg gtcaaacccg acccggagat
      121 tttccgcctg gtcctcgcgc ggctcggcgc cgttccgcga gaatcgcttg tgatcggcga
      181 cacgatcaac gacatcctcg cggcgcaggc ggtcccgctg ccggtgatcg cggtggcctc
      241 gccgtgggaa ccggccggcc gagtcctagc cgcctgccca gatttcttcc tcgactcgat
      301 ctcccagctc ccgcgtgtgc tcgacgagat caacacccgc agatcgccgg ggtgacggtt
      361 gctgcgcacg agagagttgg gcgcacagcc ggcccgaaca gacgtgtccc cctgagcgga
      421 gccggggtct cacacgggcg gcgcagtgcc tccaagaata gtcccacgcg gcttcctgct
      481 cgcggcgatt cggtcgggga gctacaacgt cacgtcgagg atctgctcgc cggtttcgtc
      541 ggcctgggcc acgatggaac catccggcgc gaccaccatc gaggaaccgc cgaaccggtt
      601 gacgccgtcg ctgccgacgg cgttgacgcc gatcacggtg gcgcccagtt cgcgggcgcg
      661 ctgcgccagc aagcgccgcc agtcttcgat gcgcacttgc ggaaaggccg cggggacgat
      721 caggtacggg acggcgagtt cggcgaggcg ggcgaagatg tcgtcgaaac ggagatcata
      781 gcagacggcg acgccgaaat cgccgaaggg cgtgacgaag aggccgggga ccttccccgg
      841 ccgatagatg cgatcctcgc cgaacggcgg gaagagattg atcttgtcgt agacccaggt
      901 ctggcggtgc cggcggaaga agtaggaatt gtaccggccc tccacctcga cgcggacggc
      961 gccgaacatg acgcccaccc cgaacggcac gagccgctgc aggatccgat ccaccggttc
     1021 caccggccgc ggctcgtaga ggcagccggt ggcggcgagc tcgccgaagc agacgaggtc
     1081 cattccggcc cggtcggccg cctcgaccca cggcacgaga tcgagctcag cgatgtgttt
     1141 ttgtacgata cgcagcttca cggcggctct cccggcgtcc acagacaata cgggacaggg
     1201 aacgtttgcg ttaaggtagg gggcgcaacg cgccaggcaa gtaaatagcg cgggaggcgg
     1261 aaaaaccgac ttaacagaag gagtcagcgg aaagggggta ccccacagct ctgcggtggg
     1321 ggctttgcgt tattcactca cagtcatgtt caccatgtcc acccgcacgg gcacgtagtg
     1381 ccttcccagg caggaattgt agctcgacca tttccagtcg gcagggcaac ggcccaccgc
     1441 agagctgcgg gcgaccagcc gctcgccttg cggccggcga gggggtgcga catcggcgga
     1501 agccgcccgc cggccgttga aaaatac
//
LOCUS       DAOOKS010000541         1525 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_107627_length_1525_cov_2.838095, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000541 DAOOKS010000000
VERSION     DAOOKS010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107627_length_1525_cov_2.838095"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1525
                     /locus_tag="PK112_08310"
     CDS             <1..>1525
                     /locus_tag="PK112_08310"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HPC12085.1"
                     /translation="KGGAEPLFFDSAGMIITLILLGRLLEAKSKGRAGEAIRSLVQLQ
                     PAKTTAIIGGTEVEIDAAAAQPGMLLRVRPGERIAADGRVVEGTPAVDESMLTGESLP
                     VEKQPGDAVIGGSLNGHSVFTFQVTAAGADSVLAKIIALVSEAQGKKAPVQKLADRVA
                     AVFVPIVLGLAALTLALWLLFAPGNDVMMRSVISVLIIACPCALGLATPTAILVGTGR
                     AAREGIIIRGGDVLERITTVDTVLFDKTGTLTHGELQATGVTALNGYREEELIALVGG
                     AESMSEHPIGRALARLREERGIPPAEVRKVEARPGFGLIGQSEGGRLVIGNQALLEQE
                     NVALAGHQEAIERTTAAGHTAVLAGLDGKLIGLFGIADRVRPEAAEVVRELQKTKQVA
                     MISGDTRRTAEKVARLAGVEHFEAEIKPDQKRLLVDSYRRAGGTVAMVGDGINDAPAL
                     AAADVGVAVGSGTDVAVETADVVLVRPDLRGVPKMFLAAAWTMRVIRQNLFWAFVYNA
                     "
BASE COUNT          251 a          449 c          594 g          231 t
ORIGIN      
        1 cgaaaggcgg ggccgagccg ctctttttcg attcggccgg gatgatcatc acgctgattc
       61 tcctggggcg gttgctggag gcgaaatcga aggggcgggc gggcgaggcg atccggtccc
      121 tggttcaact gcagccggcc aagacgaccg cgatcatcgg cggcacggag gtcgaaatcg
      181 acgccgccgc cgcgcagccg ggaatgctcc tgcgggtgcg gccgggcgag cggatagcgg
      241 ccgacggccg ggtggtggag ggaacgcccg cggtcgatga gtcgatgctg accggggagt
      301 cgctgccggt cgagaagcag ccgggggatg cagtgatcgg cgggtcgctc aacggccaca
      361 gcgtgttcac gtttcaggtg acggcggccg gcgcggactc ggtcctggcg aagattatcg
      421 cgctcgtatc ggaggcgcag gggaaaaaag cgccggtgca gaagctcgcc gaccgggtgg
      481 cggcggtctt tgtgccgatc gtgctcgggc tggcggcgct cacgctcgcg ctctggcttt
      541 tgttcgcacc cggaaacgac gtcatgatgc ggagcgtcat ctccgtcctg atcatcgcct
      601 gcccctgtgc gcttgggctg gcgaccccga cagccatcct cgtcgggacg gggcgggcgg
      661 cccgcgaagg aatcatcatc cggggcggag atgtgctcga acggatcacg acagtcgaca
      721 cggtcctttt cgacaagacc ggcacgctga cccacgggga gcttcaggcg accggcgtga
      781 ccgcgcttaa cggataccgc gaggaggagt tgatcgcgct cgtgggcggg gccgaaagta
      841 tgtcggagca cccgatcggc cgggcgcttg cccggctgcg cgaagagcgg gggatcccgc
      901 cggcggaggt gcgcaaagtt gaagcccggc ccgggttcgg cctgatcgga cagagcgagg
      961 gggggcgact cgtgatcggc aaccaggcac tgctcgagca ggaaaatgtc gctctggccg
     1021 gccaccagga ggctatcgag cggacgaccg ccgccggcca caccgccgtg ctcgctggat
     1081 tggacggcaa gctgatcggc ctgttcggca tcgccgaccg agtccgcccg gaggcggcgg
     1141 aagtagtcag agaacttcag aagaccaagc aggtggcgat gatctccggg gacacccggc
     1201 gtacggccga gaaagttgcg cggctggcgg gggtcgagca tttcgaggcg gagatcaagc
     1261 cggaccagaa acgactgctg gtcgattcct accgccgggc ggggggcacg gtggcgatgg
     1321 tgggagacgg gatcaacgat gcccctgctc tggcggcggc cgatgtgggc gtggcggtcg
     1381 ggtcggggac ggacgtggcg gtcgagacgg ccgacgtggt gctcgtacgg ccggacctgc
     1441 gcggggtgcc gaagatgttt ctggcggcgg cgtggacgat gcgggtgatc cgccagaacc
     1501 tgttctgggc cttcgtctac aacgc
//
LOCUS       DAOOKS010000542         1517 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_108469_length_1517_cov_3.256498, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000542 DAOOKS010000000
VERSION     DAOOKS010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108469_length_1517_cov_3.256498"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1517)
                     /gene="lepA"
                     /locus_tag="PK112_08315"
                     /pseudo
     CDS             complement(1..1517)
                     /gene="lepA"
                     /locus_tag="PK112_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012466592.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
BASE COUNT          240 a          515 c          478 g          284 t
ORIGIN      
        1 aactgcttca tccgcttctt cccctccttc tggcgctcga gcagtttccg cttgcgggtg
       61 atgtccccgc cgtagcactt cgcggtcacg tttttccgca tgggtttgac ggtcgcccgg
      121 ctgatgatcc ggctgccgat cgccgcctga atcacgacct cgtacatctg gcgcggaatc
      181 aactcccgca gcttctcatt gaggctcttc ccccagctgt aggcccggtc gcggtggata
      241 atgaccgaca gggcatcgac cggctctccg ttgacgagga tgtcgagctt gacgagatcg
      301 gagcgccggt agtacggcac gccgtagtcg aacgaggcgt agccgcggct gaccgacttg
      361 agcttgtcgt agaaatcgaa gatgatctcc gacaggggga aagcgtacga caggttggcc
      421 cgcgcggccg agagatactc ggtgttcttg tactcgccgc gccgctcggt cgccagcttc
      481 atgatcgccc cgacatattc gctcgggacg atgatctggg cgtcgacgta cggttcctgc
      541 acgtggtcgg tctcctgcgg cgggggcatg tccgccgggt tgtcgaccgc cgccatcgtc
      601 ccgtcggtcc ggtagacgtg gtattcgacg ttcggcacgg tgttgatgat caactgcccg
      661 tactcgcgcg agagccgctc ggtgacaatc tccatgtgca ggagtccgag aaatcccacc
      721 cggaagccga acccgagcgc ctgcgaggat tccggcgtgt acgagagcga ggagtcgttg
      781 agcgcgagtt tttcgagcgc ctcccgcagc cgcgtgaagt cctcggccac cgccgggtag
      841 aggcccgaaa acaccattgg cttgatgttg acaaagcccg gcaggggcgc ggccgccgga
      901 ttctccaccg tggtgagggt gtccccgatc ttggtgtcgg ccacctcgcg gatcgaggcg
      961 aacaggtacc ccacctcgcc ggccacgagc gcctccgccg gcgccgtgcc gagccgcagg
     1021 taccccacct cgtcgacgtc gtacgtcttg cccgtggcca tggcgcggat gcggtcgtgc
     1081 gtccgcagcg tcccgttgaa cagccgcagg agcggcatcg ccccccggta gctgtcgtaa
     1141 atcgaatcga acaccatcgc ctgcagcggc gcacccggat cccccttcgg ccccgggatc
     1201 cggcgcacga tcgcctcgag cacctcggta atccccgtcc cgtgcttggc cgaaacccgg
     1261 agcacttcct cccgcggcac gccgagcagg tcgaccagct ccgcgaccac cttctccgtc
     1321 tccgcgttcg gcaggtcgat cttgttcacg accgggagta tctcgaggtc gtgctccatg
     1381 gccagctaga ggttcgacag cgtctgggcc tcgattccct gggaggcgtc gaccaccagc
     1441 agcgcgccct cgcaggcggc cagcgagcgg ctcacctcgt aggtgaaatc cacgtggccc
     1501 ggggtgtcga tcagatt
//
LOCUS       DAOOKS010000543         1516 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_108614_length_1516_cov_2.574264, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000543 DAOOKS010000000
VERSION     DAOOKS010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108614_length_1516_cov_2.574264"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..802)
                     /locus_tag="PK112_08320"
     CDS             complement(<1..802)
                     /locus_tag="PK112_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12086.1"
                     /translation="MRRFVCLVSAFALLVSAAGTLSAETEQEIVNRYMKKVEKKHVQK
                     IGWVALNYTLNRINRNNDYNSFASSISAQIAEADLPWLGQASTFGVDFAVGVKPRLAW
                     TVGGEYWLKFGSDIAGPLTYSPPSGGATVVDNLTSEIKVYGVTTGLQYFLLNAPAPDR
                     PAAGLSARAFGAVGYYHASWELWPEYQNLNLSTAASESENSTFEGNAPGFTLGLGGEI
                     PLNVLNLSLGADFGYLYLNFTNIAWYNAQDEEIIVTYTGDQDGRVDLDL"
     gene            complement(1041..>1516)
                     /locus_tag="PK112_08325"
     CDS             complement(1041..>1516)
                     /locus_tag="PK112_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HPC12087.1"
                     /translation="GKKDLGMMMMSYGSVYVAQVALGASNGQTVKAMIEAERYNGPSL
                     IIAYSHCIAHGIDMAHSMDQEDKAVRCGHWMLYRYNPDLIEQGKNPLQLDSKPPSIPF
                     SEYAYSEIRFRSLKAIDPERAAMLLKLGQQDCDRRWRLYSQLAGLDYSLPPRAGD"
BASE COUNT          277 a          402 c          551 g          286 t
ORIGIN      
        1 agaggtcgag atcgacgcgg ccatcctggt caccggtata ggtgacgatg atctcctcgt
       61 cttgagcatt ataccaggca atgttggtga agttcaggta gaggtacccg aagtcggcgc
      121 cgaggctgag gttgaggacg ttgagcggga tctcaccgcc gaggcccagg gtgaaaccgg
      181 gggcgttgcc ctcaaaggtc gaattctcgc tctcggaagc cgcggtggag aggttgaggt
      241 tctgatactc gggccagagt tcccaggacg cgtggtagta cccgacggcg ccgaaggcgc
      301 gggcggacag gccggcggcc ggccgatcgg gagcgggcgc attgaggagg aagtactgca
      361 gcccggtggt cacgccgtag actttgattt cgctggtgag gttatcgacc accgtggcgc
      421 cgccgctggg aggggaatag gtgagggggc cggcgatatc ggagccgaat ttcagccagt
      481 attctccgcc gaccgtccat gcgagccggg gcttgacgcc cacggcgaag tcgacgccga
      541 aagtggacgc ctggcccagc caggggagat cggcctccgc aatctgggcg gagatggagc
      601 tggcgaaact gttgtagtcg ttgttgcggt tgatacgatt cagggtgtag ttgagcgcca
      661 cccagccgat tttctgcacg tgcttcttct caactttttt catgtagcgg ttgacgatct
      721 cctgctcggt ttccgccgag agggttccgg ccgcagacac taggagcgca aaggcgctca
      781 ccaggcacac gaaacgacgc atggcaacct cctgtgaact ggtaagaatc agtttgtgat
      841 tttatcggaa ccgctttcgg gggacttgac ggaagagcgt gtcttcgaag gcgttcgcgc
      901 aaaaatagga cgggcggagg cggggcgcgg gattgttcag attgtgcaca gcggagcggg
      961 gcggccgggc cggagcccaa agaaaacggg gctcgcgaag agccccgtgg aaatggcaca
     1021 gaaaacggcc ggtcgggcgg ctagtccccg gccctgggcg gcagggagta gtcgaggccg
     1081 gccagttggg agtacagccg ccagcggcgg tcacagtcct gctggccgag tttcaggagc
     1141 atggcggccc gctccgggtc gatggctttg agcgaccgga agcggatctc gctgtaggca
     1201 tactcggaga aggggatgga cggcggcttg ctgtcgagct ggagcgggtt cttcccctgc
     1261 tcgatgagat ccgggttgta gcggtagagc atccagtggc cgcagcggac ggccttgtct
     1321 tcctggtcca tggagtgggc catgtcgatg ccgtgggcga tgcagtggct gtaggcgatg
     1381 atgagcgagg gaccgttgta gcgctcggcc tcgatcatgg ctttgacggt ctggccgttg
     1441 ctggcgccga gggcgacctg ggccacatag acgctgccgt acgacatcat catcatgccg
     1501 aggtccttct tgccgc
//
LOCUS       DAOOKS010000544         1515 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_108718_length_1515_cov_2.974658, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000544 DAOOKS010000000
VERSION     DAOOKS010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108718_length_1515_cov_2.974658"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            185..955
                     /locus_tag="PK112_08330"
     CDS             185..955
                     /locus_tag="PK112_08330"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPC12088.1"
                     /translation="MHVLAFHKLTRRLTFGSTNYRPDRFRRLLVSLLTDGYTFVPLDG
                     AGERRTTGERELAITFDDGYGHLVDALPGLMEEFGFRPTVFVPTGYLGRPNAWDYSFV
                     FRAEPHLTAGDIRALSACGVAFGSHGHTHTDLTRLSDSTLIEQLRVSRAILEDLTGYP
                     VTALSYPFGRVDSRVSEYARAAGYCCGYTLRFPSLADTLFTAGRYGVWCFDTPGSVRR
                     KLRPGPLQSFERLKARVTHALSAGTRLTHRRVRGTARS"
     gene            1066..>1515
                     /locus_tag="PK112_08335"
     CDS             1066..>1515
                     /locus_tag="PK112_08335"
                     /inference="COORDINATES: protein motif:HMM:NF020892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HPC12089.1"
                     /translation="MGTRKTETMRFPYREIEAKWQQRWIERGVHRAPRMPDPARKWYQ
                     LEMFAYPSGDIHMGHFRNYVIGDAVARQRLMLGADLLHPFGWDAFGLPAERAAIQRGV
                     PPAEWTRSNVGVSRATLQRTGVSFDWTREIDSSRPDYYRWTQWMFIQL"
BASE COUNT          221 a          572 c          441 g          281 t
ORIGIN      
        1 ggaatttgcc aacgagtcgt tcgatcgctc ccagctcacc gtcgtcaccc tcggacccgc
       61 cccgcaccgc cgcctccgcg aggtcacggg agccggggcg aagcgctgac cggcgccgcg
      121 tccccgggtc gcgaggtcgc ggcggacact tgacggcctc gctatcgccc cctacgttac
      181 ccccatgcac gtcctcgcct tccacaaact cacccgccgc ctcaccttcg gctccaccaa
      241 ttaccggccc gaccgtttcc gaaggctgct cgtttcgctc ctcaccgacg gctacacctt
      301 tgtccccctc gacggggcgg gggagaggcg cacaacgggg gagagggaac tggcgatcac
      361 gttcgatgac gggtacggcc acctggtcga cgcgctcccc gggttgatgg aggaattcgg
      421 ctttcgcccg accgtttttg tccccaccgg ataccttggc cgccccaacg cctgggacta
      481 tagctttgtc ttccgcgccg aaccgcacct gacggccggg gacattcgcg ctctctcggc
      541 gtgcggtgtc gcttttggct cccacggcca cacccacacc gatctgaccc gcctttcgga
      601 ttcaactctg atcgaacagc tccgcgtgtc ccgcgcaatt ctggaggatc tcaccggcta
      661 tccggtgacc gctctcagct accccttcgg acgggtcgat tcccgggtgt ccgagtacgc
      721 gcgcgccgcc ggctattgct gcggctacac gctgcgcttt ccctcgttgg ccgacacgct
      781 gttcacggcc ggccgttacg gcgtgtggtg tttcgatacc ccgggttcgg tccggcgcaa
      841 actcagaccc ggtcccctgc aatcgttcga gcgtctcaaa gcccgggtca cccacgcgct
      901 ctcggccggg acccgcctca cccatcggcg cgtcagggga accgctcgtt cgtagcggcg
      961 ccgcgggtcg gccgcctctg cacgcggcgc tcaaactaac ccttgcctcc tccgccgcat
     1021 tgtgctactt tcgtccctac tctggagtga ccgaggatgt tgtcgatggg gacccgtaag
     1081 actgaaacca tgcgctttcc gtacagagag atcgaggcca agtggcagca gcggtggatc
     1141 gagcggggtg tgcaccgcgc cccccgcatg cccgatcccg cccgcaagtg gtaccagctc
     1201 gagatgttcg cctatccctc gggcgacatc cacatgggcc atttccgcaa ctatgtcatc
     1261 ggcgatgccg tggcgcgcca gcgcctcatg ctcggcgccg acctcctgca cccgtttggc
     1321 tgggatgctt tcggcctgcc ggccgagcgc gccgccatcc agcgcggcgt cccgcccgcc
     1381 gagtggaccc gaagcaacgt cggcgtgtcc cgcgccacgc tccagcgaac cggcgtctca
     1441 ttcgactgga cccgcgagat cgattcctcc cgccccgact actaccgctg gacgcagtgg
     1501 atgttcattc agctc
//
LOCUS       DAOOKS010000545         1511 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_109130_length_1511_cov_3.644918, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000545 DAOOKS010000000
VERSION     DAOOKS010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109130_length_1511_cov_3.644918"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..988)
                     /locus_tag="PK112_08340"
     CDS             complement(<1..988)
                     /locus_tag="PK112_08340"
                     /inference="COORDINATES: protein motif:HMM:NF012879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPC12090.1"
                     /translation="MSKPVPVIAVLLGALSALVAGPAGSCRAAETYELDNGMTVILKE
                     QHGAPMVSSIVFVRSGSKYESRFENGITHFLEHLLFDGTATLAREQLDASIGDLGGYI
                     NAFTRKELTAYLVLLPKQYIEYGMTVQADMLFNSVFPEAELAKERKVVVEEIRRDADA
                     PGAAAEAFFTDRAYAGTDYARPVLGYPAFIENIPRAAVIDYWRRYYIPRRMTLLVIGD
                     FEPAAMKKTVANVFGRFTNPASAADSAASASPAPAPVLEGQTIYDTAAQVPSTYLNLS
                     LAAPPIGSAEYYAMDLLARYLAMDAVSPLMVALKGGAEPLATEASVSLVPYEE"
     gene            1145..>1511
                     /locus_tag="PK112_08345"
     CDS             1145..>1511
                     /locus_tag="PK112_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12091.1"
                     /translation="MTKRDRIELLRRSARTGLTGLVFLIIGGTGLFVAGITWSAGTDW
                     EGPGDGYACTYPPAGHGGQQSMTPNGQPPGEPSDHLILPLIIKTDNSDKRPAAADRAY
                     FHTCRRAGEVRDRTSPCSST"
BASE COUNT          259 a          462 c          544 g          246 t
ORIGIN      
        1 attcctcata ggggacaagg ctcaccgacg cctcggtggc cagcggctcg gcgcccccct
       61 tcagcgcgac catgaggggg gagacggcgt ccatcgcgag gtaccgggcg agcagatcca
      121 tggcgtagta ttccgcgctg ccgatgggcg gggcggcgag ggagagattc aggtaggtgg
      181 agggaacctg cgccgccgtg tcgtagatgg tctgtccctc gaggacgggg gcgggagcgg
      241 gcgaggcgga tgcggcggag tccgccgccg aggccgggtt ggtaaagcgg ccgaagacgt
      301 tggccaccgt cttcttcatc gccgcgggct cgaaatcgcc gatcaccagc agcgtcatgc
      361 ggcgcggaat gtagtagcgc cgccagtagt cgatcacggc ggcgcggggg atgttctcga
      421 tgaacgccgg gtagccgagc acggggcgag cgtaatccgt gccggcgtag gcccggtctg
      481 tgaaaaacgc ctcggctgcc gcaccgggcg catcagcgtc gcggcgaatc tcctccacca
      541 cgaccttgcg ttccttcgcc aactccgcct ccgggaagac cgagttgaag agcatgtcgg
      601 cctgcacggt catgccgtac tcgatgtact gcttcggcag gagtaccagg taggcggtga
      661 gctccttgcg ggtgaaggcg ttgatgtagc cgccgagatc gccgatcgag gcgtcgagct
      721 gctcgcgggc gagggtcgcc gtgccgtcga aaagcaggtg ctcgagaaag tgggtgatgc
      781 cgttctcgaa ccgggactca tacttgcttc cggagcggac gaacacgatg ctggagacca
      841 tgggcgcgcc gtgctgctct ttgaggatca cggtcatgcc gttgtccagc tcgtaggttt
      901 cggcggcacg gcaggagccg gcggggcctg ccaccagggc cgaaagcgcg ccgaggagga
      961 cggcgatgac ggggacgggt ttcgacatat gaactcccgg ttctttttgc cttcgcggcg
     1021 gtttggtcga gccgacgatc tgccgatgct aaggtatacc ggggcgggag ggatgtcaac
     1081 cggcccgcgg gggcggaatt ttggcgttga caaccccgga taacatggta tattcacgca
     1141 acttatgacg aaacgggacc ggatagaact gctccgccgg agcgcccgga ccggcctcac
     1201 cgggctggtc tttctgatta tcggcggcac gggcctgttt gtcgccggga taacttggtc
     1261 ggcggggacc gactgggagg ggccgggcga cgggtacgcc tgcacctacc ccccggcggg
     1321 ccacgggggg cagcagagca tgaccccgaa cgggcagccg ccgggggaac cctcggacca
     1381 tctcatcctc ccgctcatca tcaagaccga caactcagac aagcgtccgg cggccgccga
     1441 ccgggcgtat tttcacacct gccgccgggc gggcgaagtg cgcgaccgca cctccccctg
     1501 cagttcgacc g
//
LOCUS       DAOOKS010000546         1510 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_109277_length_1510_cov_2.875601, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000546 DAOOKS010000000
VERSION     DAOOKS010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109277_length_1510_cov_2.875601"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1095)
                     /locus_tag="PK112_08350"
     CDS             complement(<1..1095)
                     /locus_tag="PK112_08350"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HPC12092.1"
                     /translation="MNNSDQLFTADLHCDTIHRILQGADFSRRNEIGHLDLPRLIEGG
                     IDLQVFACFTESFIEAKDRVPLVHRMLDGLHRTVEAHGEKVALARTSSEAARNRAAGK
                     ISAVFSIENGLVLNDNLDTLRQFYERGVRSLTLTHNVSSAWCISSADEHPAFDGLTAF
                     GREVVRMMDELGMIVDLSHAAPSAVAAVLEIATRPVIASHSCVRALCDHHRNLTDEQI
                     RGIAATGGMVGINFLCDFLSPACRDASAAYINAHYDLGRRYWLLFTAECGAEEYAARR
                     AELQPFLAGWEQVVRATGVDVGVVADHIDYIVRLVGPDHVGLGSDFDGITFAPAGLED
                     CAKMPRLADELRGRGYCREDLAKIMGGNFLR"
     gene            1284..>1510
                     /locus_tag="PK112_08355"
     CDS             1284..>1510
                     /locus_tag="PK112_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XylR N-terminal domain-containing protein"
                     /protein_id="HPC12093.1"
                     /translation="MRGERLRRAAAGAPPAGEAGFDLSRELSFHPDTGRTLFRDSRVL
                     ILDANAIGMLRQNLLDTLGWAQARDFLLRFG"
BASE COUNT          266 a          501 c          499 g          244 t
ORIGIN      
        1 gcggaggaaa ttgcccccca tgatcttagc taggtcttcc cggcaatatc cccgcccccg
       61 cagttcgtcg gccagacgcg gcattttcgc acaatcctca aggccggccg gggcaaacgt
      121 gatcccgtcg aagtccgacc ccaggccgac atggtccggg ccgaccaggc ggacaatgta
      181 gtcgatgtgg tcggccacca cccccacatc gacccccgtg gcccgcacga cctgctccca
      241 gcccgcaaga aacggctgca gctccgcccg ccgggccgcg tactcctcgg cgccgcactc
      301 ggccgtgaac agcagccagt atctcctgcc cagatcatag tgggcgttga tgtaggcggc
      361 ggaagcgtcg cggcaggctg gggagagaaa atcgcagagg aagttgatgc cgaccatgcc
      421 gccggtcgcg gcgattcccc ggatttgctc gtcggtgaga ttccggtggt ggtcgcagag
      481 agcgcggacg cacgagtggg aggcgatcac cggccgggtg gcgatctcca gtacggccgc
      541 caccgccgac ggggccgcgt gggagaggtc gacgatcatg ccgagttcgt ccatcatccg
      601 caccacctcc cgtccgaaag cagtcagacc gtcgaacgcc gggtgctcat cggccgagga
      661 aatgcaccac gcgctcgaga cgttgtgggt cagggtcagg cttcggacgc cccgctcata
      721 gaactgccgg agagtgtcca ggttgtcgtt gagcaccagt ccgttttcaa tcgagaagac
      781 ggcgctgatt ttgcccgcgg cgcggttgcg cgcggcctcg gatgaggtcc gggcgagggc
      841 gaccttctcc ccatgggcct ccacggtgcg gtgcagcccg tcgagcatcc ggtggaccag
      901 cgggacccgg tccttcgcct cgatgaacga ctccgtgaaa caggcgaata cctgcagatc
      961 gattccgcct tcgatcaggc ggggaagatc gaggtgtccg atctcattgc gccgggagaa
     1021 atcggcgccc tggaggatcc ggtgaatcgt gtcacagtgc agatcggcgg tgaagagttg
     1081 atcgctgttg ttcataacgt cctttccgga gccaagatta cacagaaatg gagcgtcagg
     1141 caagttccgt cggggccgga gtcaaattcg gcctatcggg agccgataag agaggcgtgg
     1201 acatcgtttc gaccaaaaga aaccggccgg ccgcccgcgg ccgacggccc caggaggcgg
     1261 gccggcggga gggctgggtc actgtgcggg gggaacgcct ccgccgcgcg gccgccggcg
     1321 cgccgcccgc gggcgaggcc gggttcgacc tttcccgcga actgagcttc catcccgaca
     1381 ccgggcgcac cctctttcgc gacagccgcg tcctgatcct cgacgccaac gcgatcggca
     1441 tgctccgcca gaacctcctc gacactctcg gctgggcgca ggcccgcgac tttctcctgc
     1501 ggttcggcta
//
LOCUS       DAOOKS010000547         1505 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_109852_length_1505_cov_3.161379, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000547 DAOOKS010000000
VERSION     DAOOKS010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109852_length_1505_cov_3.161379"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1237)
                     /locus_tag="PK112_08360"
     CDS             complement(<1..1237)
                     /locus_tag="PK112_08360"
                     /inference="COORDINATES: protein motif:HMM:NF017039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HPC12094.1"
                     /translation="MTGPRDTDDSLTPLMRQYYAVKAQHPGKILFFRMGDFYEMFGDD
                     AVKAAPILGIALTSRSHGANERVPLAGVPYHSADKYLARLLAAGEKVVVVEQVEDPRT
                     AKGLVKREIVEILTPGTATIEGEASDRQAVWLAAVCPRGDSALGLAALDLSTGAFLVD
                     EGPTDEVRERLRVLEPREVLYPTGGEAEDGRPGFDPANGAIHLTGFAEWNFDYRTAVR
                     ELSEHFGTSTLDGFGIGEARLGIAAAGAVFRYLRENHRERLSHIDRIVRFDTAEYMVL
                     DYGTVRNLELLRNLAEGTEPNSLFAAVNRCRTAPGARRLAAALVRPFRNKARIEKRLS
                     GVAELVRDRDLAARVREHTQRLPDLEKIAGRLGIGKLNPRQMKAIGEGLAECRALGRL
                     IAAAHSPLLTELAAALPDTT"
     gene            complement(1234..>1505)
                     /locus_tag="PK112_08365"
     CDS             complement(1234..>1505)
                     /locus_tag="PK112_08365"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HPC12095.1"
                     /translation="VERRAAEAEQVTGTYYTIQVGVFTDADIARTQAERFRQTGWKTE
                     IADKTISRVTYRAVYVGRFAAYQEAAAAKARLEREMNETYQVVAR"
BASE COUNT          210 a          548 c          499 g          248 t
ORIGIN      
        1 cggtggtatc ggggagggcc gccgcgagtt cggtcaatag cgggctgtgc gccgcagcga
       61 tcaggcggcc gagcgcgcgg cactcggcca gtccctcgcc gatcgctttc atctggcgcg
      121 ggttcagctt cccgatcccc agccgcccgg cgatcttctc caggtccggc agccgctggg
      181 tatgttcgcg cacgcgggcg gcgaggtccc ggtcgcgcac cagttcggcc acgcccgaga
      241 ggcgcttctc gatccgcgct ttgttgcgga agggccgcac gagagcggcc gccagccgcc
      301 gcgcccccgg ggccgtccgg cacctgttca ccgcggcgaa gagcgaattc ggctcggtcc
      361 cctcggcgag attgcgcagc agctccagat tccgcaccgt cccgtagtcc agcaccatgt
      421 actccgcggt gtcgaaccgc acgatgcggt cgatgtgcga caaccgctcg cggtggtttt
      481 cgcggaggta gcggaacacc gcccccgcgg ccgcaatccc caggcgcgcc tcgccgatcc
      541 cgaatccgtc gagcgtgctc gtcccaaaat gctcgctgag ctcccgcacc gccgtccggt
      601 agtcgaaatt ccactccgcg aacccggtca ggtggatcgc gccattggcc ggatcgaacc
      661 cgggccggcc gtcctccgct tccccgccgg tggggtacag aacctcgcgc ggttcgagga
      721 cgcgcaggcg ctcgcgcacc tcgtcggtcg gcccctcgtc gacgaggaac gccccggttg
      781 acaggtcgag cgcagccagc ccgagcgcgc tgtctccccg cgggcagacg gccgcgagcc
      841 acaccgcctg ccggtccgag gcctctccct cgatcgtcgc cgtccccggc gtcaggattt
      901 ccacgatctc gcgtttgacc agccctttgg cggtcctggg gtcctcgacc tgctcgacca
      961 ccaccacctt ctcccccgcc gcgagcaggc gggcgaggta cttgtcggcc gaatggtaag
     1021 gcacgccggc aagcggcacg cgctcgttgg cgccgtggga ccgcgaggtc agcgcaatcc
     1081 ccagaatggg ggctgccttc accgcgtcgt cgccgaacat ctcatagaaa tcccccatgc
     1141 ggaaaaacag gatcttgccc ggatgctgcg ccttcaccgc gtagtactgg cgcatgaggg
     1201 gagtaaggga atcgtctgtg tcccgcgggc ccgtcatcgc gcgacgacct ggtaggtttc
     1261 gttcatctcg cgttcgaggc gggctttggc ggcggccgcc tcctggtagg cggcgaaccg
     1321 cccgacatac accgcccggt aggtcacgcg ggagatggtc ttgtcggcga tctcggtctt
     1381 ccagccggtc tgccggaagc gctcggcctg ggtgcgggcg atgtcggcat cggtgaacac
     1441 ccccacctga atggtgtagt acgtgccggt cacctgttcg gcctcggcgg cgcggcgctc
     1501 gactc
//
LOCUS       DAOOKS010000548         1505 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_109869_length_1505_cov_2.774483, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000548 DAOOKS010000000
VERSION     DAOOKS010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109869_length_1505_cov_2.774483"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="PK112_08370"
     CDS             complement(<1..480)
                     /locus_tag="PK112_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008082416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPC12096.1"
                     /translation="MSAKKAAKKPAAKAAAGGENPRGTRAAQARGRRGVKTPHELREN
                     GIALHQEEIAYQMALRQREISVSEFFTKNRHLLGFDNPKKALLTTVKEAVDNSLDACE
                     EAKILPELTVEVRQTGEDRYRVAVEDNGPGIVRSQVPRIFGKLLYGSKFHALKQARGQ
                     "
     gene            complement(659..1003)
                     /locus_tag="PK112_08375"
     CDS             complement(659..1003)
                     /locus_tag="PK112_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016780356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12097.1"
                     /translation="MKKAIVALMILAVAASPALAKKHGKPRHSRKSNAALCFPYGDTA
                     VAAATAFSIIFDSIKGVDRYYPVPVAQPVYVPQPVPVSVCPPQPVYYQAYQPVACNLP
                     AAYAPVPYYPVR"
     gene            complement(1224..>1505)
                     /locus_tag="PK112_08380"
     CDS             complement(1224..>1505)
                     /locus_tag="PK112_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12098.1"
                     /translation="PDWSPDGESIVYSSRQGGFGIWLVNLKTREEIPLPRSGSSDEAP
                     SWAPDSRHIVYCVQRGRKRDLFIMDVYDRAPTQITRGPGNFSSPAWAPR"
BASE COUNT          204 a          487 c          544 g          270 t
ORIGIN      
        1 ctggcccctg gcctgcttga gggcgtggaa cttggagccg tagaggagct tgccgaatat
       61 ccgcggcacc tggctcctga cgatcccggg gccgttgtcc tcgaccgcga cgcggtaccg
      121 gtcctcgccg gtctggcgca cctccaccgt gagttcgggg agtatcttgg cctcctcgca
      181 cgcgtcgagg ctgttgtcca ccgcctcctt gaccgtggtg aggagcgcct tcttcggatt
      241 gtcgaaaccg agcaggtgcc ggttcttcgt gaagaactcg gagacggaga tctcgcgctg
      301 ccggagcgcc atctggtagg cgatctcctc ctggtggagg gcgatgccgt tctcacggag
      361 ctcgtgcggg gtctttaccc cgcgccggcc ccgcgcctgg gcggcgcgcg tcccccgggg
      421 gttttccccg cccgcggctg ctttcgcggc cggttttttc gcggctttct tcgccgacat
      481 tgtgtcgtgt tccccgatcc aattgcgacg attcggcata gtagcatact gatgccgtcg
      541 cctcaaggag gatcgggaac cggttcggga gaagccgccg atcggaccgg gcggggcgcc
      601 ggacgaggcg tcccgccgga tccgctgcgt ttcccccgaa cccctccgcg gcttccggtc
      661 agcggaccgg atagtacgga acgggcgcgt acgccgcggg gaggttgcag gcgaccggct
      721 ggtacgcctg gtagtagacc ggctgtggcg ggcagaccga gactggcacc ggttgcggca
      781 cgtagaccgg ttgcgccacg ggcaccgggt agtacctgtc gacgcccttg atgctgtcga
      841 agatgatgct gaaggccgtg gccgccgcca ccgcggtgtc gccgtagggg aaacagaggg
      901 ccgcgttcga ttttcgcgag tgccgcggtt ttccgtgctt tttcgcgagg gcggggctcg
      961 ccgccaccgc gaggatcatc agcgccacga tcgccttttt catcgcaccc tcctccctgt
     1021 tggcccggcc gtcgccgcac cgcggctgtt catggagtta tacgacaggt cggcccgcgc
     1081 catccccgcc tgcagccggc atatttcagc ggaggatggc gcgtggagat tcgcccggtg
     1141 acgaaacgga gcggaacgcg gcgccggggg gagcaggatg gcgccccctg tccgaggcgc
     1201 gcgggtcggg cacgacgtct gcgtcagcgg ggggcccagg cgggggagga gaagttgccg
     1261 ggaccgcggg tgatctgcgt cggcgcgcgg tcgtagacgt ccattatgaa cagatcgcgc
     1321 ttcctgccgc gctggacgca gtagacgatg tgccggctgt cgggggccca ggagggcgcc
     1381 tcgtcgctgg agcccgagcg gggcaggggg atctcctcgc gcgtcttcag gttgaccagc
     1441 cagatgccga agccgccctg ccgggaactg tagacgatcg attcgccgtc cggcgaccag
     1501 tcggg
//
LOCUS       DAOOKS010000549         1504 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_109969_length_1504_cov_3.674258, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000549 DAOOKS010000000
VERSION     DAOOKS010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109969_length_1504_cov_3.674258"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="PK112_08385"
     CDS             <1..452
                     /locus_tag="PK112_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SecY family transport protein"
                     /protein_id="HPC12099.1"
                     /translation="LFPNTDWVQNLVAVWLAPGGLWYSLIYGVIIIFFAYFYTAIVFN
                     PMEIADNMRKNGGYIPGIRPGKNTADYIEKILTRITLPGAIFFAAIAILPWVLIGQFN
                     VNFFFGGTGLLIVVGVALDTLQQIESHLIMRHYDGFMKKGRIRGRSM"
     gene            452..1105
                     /locus_tag="PK112_08390"
     CDS             452..1105
                     /locus_tag="PK112_08390"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008287617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HPC12100.1"
                     /translation="MRHLVFLGPPGSGKGTQAAKLAAEMKIRYLSTGDLLRAAVSTGT
                     ELGRKARGYMERGELVPDALLVGLIEATIRAGELDHGFILDGFPRTVPQAEALRDMLN
                     GNGKELDAAVLLAVSDEEVVRRLSGRWFCPSCQATYNYPHHMPRREGRCDAEGAALER
                     RPDDQPEVVRRRLDVYKKQTRPIEDFYRAAAILVEVQGEQPPEAVFADLLDIVGARK"
     gene            1122..>1504
                     /locus_tag="PK112_08395"
     CDS             1122..>1504
                     /locus_tag="PK112_08395"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HPC12101.1"
                     /translation="MIVLKTAEQIEVMARAGRVVGRTLDMVGTVIRPGMTTGQLDRLI
                     EDFIRAEGAIPAFKGYQGFPASACISIDDEVVHGIPGKRVIEEGVIVSVDIGAIVDDY
                     YGDSARTFGIGEVSEEKRRLMEQAV"
BASE COUNT          268 a          443 c          541 g          252 t
ORIGIN      
        1 agctgttccc caacaccgac tgggtgcaga acctggtggc ggtgtggctg gcgccgggcg
       61 gcctctggta ctccctcatc tatggggtga tcatcatctt cttcgcctac ttctacacgg
      121 cgatcgtgtt caaccccatg gagatcgccg acaacatgcg caagaacggc gggtacattc
      181 caggcattcg gccgggcaag aacaccgccg actatattga gaaaatcctc acccggatta
      241 ctctgccggg cgccatcttc ttcgccgcca tcgccatcct cccctgggtg ctcatcggcc
      301 agttcaacgt gaacttcttc ttcggcggca ccgggctcct catcgtggtc ggcgtggccc
      361 tcgacaccct ccagcagatc gagtcgcacc tgatcatgcg gcactacgac gggttcatga
      421 agaaagggcg aatccgggga aggtccatgt gatgaggcat ctcgtgtttc tcggcccgcc
      481 cggatcgggc aaagggacgc aggcggcgaa gctggcggcg gagatgaaga tccgctacct
      541 gtcgaccggg gacctgctgc gcgccgcggt gagcaccggg accgagctgg gccgaaaagc
      601 gcgagggtat atggagcgcg gggaactcgt cccggatgcg ctcctcgtgg gattgatcga
      661 ggcgaccatc cgggccgggg aactcgacca cggattcatt ctggacgggt ttccgcggac
      721 ggtgccgcag gcggaggcgc tgcgggatat gctgaacggc aatggcaagg aactcgatgc
      781 ggccgtcctc ctggccgtgt cggacgagga agtggtgcgg cggctgtcgg ggcggtggtt
      841 ctgcccgtcc tgccaggcga cctacaacta cccgcaccac atgccccggc gggagggccg
      901 gtgcgacgcg gagggcgcgg ccctggagcg gcgcccggac gaccagcccg aggtcgtgcg
      961 ccggcgactg gatgtgtaca agaaacagac gcggccgatc gaagatttct accgggcggc
     1021 ggccattctg gtcgaggtgc agggggaaca gccgcccgag gcggtgtttg ccgacctgct
     1081 cgacatcgtc ggggcgcgaa aataggacgg aagcgacgtt tgtgatagtg ctgaagacgg
     1141 cggaacagat cgaggtgatg gcccgggcgg gccgggtggt cgggcggacg ctcgacatgg
     1201 tcggcaccgt catccggccg ggaatgacga ccgggcagct cgaccgcctc atcgaggact
     1261 tcatccgggc cgagggagcg atcccggcct tcaaggggta ccagggattc ccggcctcgg
     1321 cgtgcatttc gatcgacgac gaggtcgtgc acggaattcc ggggaaacgg gtgatcgagg
     1381 agggggtgat cgtgtcggtc gatatcggcg cgatcgtgga tgactactac ggggactcgg
     1441 cgcggacctt cggcatcggc gaggtgtcgg aggagaagcg gcggctcatg gagcaggcgg
     1501 tgat
//
LOCUS       DAOOKS010000550         1497 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_110815_length_1497_cov_2.696949, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000550 DAOOKS010000000
VERSION     DAOOKS010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1497
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110815_length_1497_cov_2.696949"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(161..550)
                     /gene="rplL"
                     /locus_tag="PK112_08400"
     CDS             complement(161..550)
                     /gene="rplL"
                     /locus_tag="PK112_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014996120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HPC12102.1"
                     /translation="MANPAIEEIVDKIAGLTAMDLADLSKAIQDKFGVTAAAPMAMMP
                     GMMAGGGGTAAVAEEQTEFDVVLTAVGDKKIQVIKVVRELTSLGLKEAKDLVEGAPAK
                     VKEGLSKDEAMKAKEKLEEVGAQVDIK"
     gene            complement(602..1165)
                     /gene="rplJ"
                     /locus_tag="PK112_08405"
     CDS             complement(602..1165)
                     /gene="rplJ"
                     /locus_tag="PK112_08405"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HPC12103.1"
                     /translation="MAKPEKVTAVAEMKKLFDECDSFFVTDYQGLSVADVTALRKSLR
                     EQGVTFLVGKNTLLKRAAHEAGVGEIDSFLVGPTAIAFARADASAAAKVLQDSFKERE
                     LPRTKVFVVADRVYGAGDLKALAELPPREQLLAQVAAAVEAPLAELVRTIDAFFQQLV
                     GSVDALAEKRKAEGPAGPPPGEAGAAS"
     gene            complement(1184..>1497)
                     /locus_tag="PK112_08410"
     CDS             complement(1184..>1497)
                     /locus_tag="PK112_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004807404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HPC12104.1"
                     /translation="PRGLMPNPKTGTVTMDLSRAVKELKAGRIEFRIDRQSNVAVSIG
                     KVSFEANQLKENVETFIAAVARAKPASAKGTYFLSASLCSTMGPGVKLDHSLLLADLK
                     K"
BASE COUNT          226 a          536 c          432 g          303 t
ORIGIN      
        1 tcccggccgt ccggtcgcgg gagcacctgc tgcagtcaat ccaccagccg aattcgaaaa
       61 aaatgggatc gctaagacct cacgctgacg gtatggtgtg gtaacccgcc gggacccggt
      121 ttgttcccgg cgggcgtttc ttttccgacg cccccggggg ctacttgatg tcgacctggg
      181 cgccgacttc ctcgagcttc tccttggcct tcatcgcctc gtccttcgac aagccttcct
      241 tcactttcgc cggggctcct tcgacgaggt ctttggcctc tttcaggccc agcgacgtga
      301 gttcgcgcac gaccttgatg acctggatct tcttgtcgcc gaccgccgtg agcacgacgt
      361 cgaattcggt ctgctcttcg gccacggccg cggtcccgcc gccgccggcc atcatccccg
      421 gcatcatcgc catcggggcc gccgccgtca cgccaaactt atcctgaatg gccttggaca
      481 gatccgccag gtccatcgca gtcagtccgg caatcttgtc gacaatctct tcaattgcag
      541 ggttagccac ggttcgtacc tcttccgttg aatgtaaagt tacacctttc gtattgcctc
      601 gtcatgacgc cgccccggcc tctccgggcg gcgggccggc cgggccctcc gccttccgct
      661 tctcggccag cgcgtcgacc gagccgacca gttgctggaa gaacgcgtcg atcgtccgca
      721 ccagttcggc cagaggcgcc tcgaccgccg ccgccacctg ggccagcaac tgctcgcgcg
      781 ggggcagctc ggcgagcgcc ttgagatcgc ccgccccgta gacccggtcg gccaccacga
      841 acaccttcgt ccgcggcagc tcccgctcct tgaacgagtc ttggagcacc ttggcggccg
      901 ccgatgcatc ggcgcgcgcg aacgcgatcg cggtcggccc gaccaggaag gagtcgatct
      961 cccccacccc ggcctcgtgc gccgcccgct tcaacagcgt attcttgccc accaggaagg
     1021 tgacgccctg ttcccgcagc gacttgcgca gcgcggtgac atcggccacg ctcagcccct
     1081 ggtagtccgt cacgaaaaag gagtcgcact cgtcgaacag ctttttcatc tcagcgacgg
     1141 cggtaacttt ttccggtttt gccatacgat tgctccttct ccgtcacttc ttgagatccg
     1201 ccagcagcag gctgtgatcg agtttgaccc cgggacccat ggtcgagcag agcgacgcgc
     1261 tcaggaagta ggtccctttg gccgaggccg gcttggcccg ggcgacggcc gcgatgaagg
     1321 tctcgacatt ttctttgagt tggttcgcct cgaacgacac cttgccgatt gagacggcca
     1381 cgttcgactg ccggtcgatg cggaactcga tgcgcccggc cttgagctcc ttgaccgccc
     1441 ggctcaggtc catcgtcacc gtccccgtct tcgggttggg catgagcccg cgcgggc
//
LOCUS       DAOOKS010000551         1495 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_110995_length_1495_cov_3.638194, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000551 DAOOKS010000000
VERSION     DAOOKS010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1495
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110995_length_1495_cov_3.638194"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            316..1485
                     /locus_tag="PK112_08415"
     CDS             316..1485
                     /locus_tag="PK112_08415"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPC12105.1"
                     /translation="MSKIVNWTIRFRLALWDFLRFHPACRFAASAAIRLYLRLPALRR
                     DESLLRAARLNVRAARICPHPGLLRRLERSLDASVGRLLRTNFDWDALSHESRPDQVA
                     KGIILKHPVSPREKGVLFISFEGQWLRLFRHADIDRLARDYHLVIAPSWSPPHSLAFS
                     VAARRWPGRFFTLISNLNEIPVFARIAPNSEVVPLFGSSWVHPQIFDVSAPVAKEYDI
                     VILANFAAYKRHFLLFRALRDMRPDTRVLLLGKSMDGRTPETIMNEARAYGVADRITI
                     KPGLPDGEMIRAFRSARVALMLSGNEGSCVAVVESMFADIPIGVFEDAVIGSLAYVNE
                     HTGRRLRRRRLGRQLEEFIADSGRYSPRRWVMENGISCFGSTAVLNDALRARVLE"
BASE COUNT          219 a          575 c          432 g          269 t
ORIGIN      
        1 gaggcctaca atgtcgtcaa agccgccgag gccggactcg aaggcaaccc ccacgactac
       61 cgcgactacc tcctgaaacc gtacctctac cctgagaccg cggtcgggaa ggactctctc
      121 gccgccgccg ttccggcggg ttgagatcgg cgcggacggg ctccttggcg ggcggcgccg
      181 agaggacacc gggcgccgga atttggccga gctccgtggg aatcagtttg ccgccggccg
      241 gcggcgcgaa tatattggcc ctgcgattcc cgaccaggcg cgtcggcctg tcccgcatag
      301 ccgccgcagc aggagatgag caagatcgtg aattggacca taagattccg cctggccctg
      361 tgggacttcc tgcgtttcca cccggcctgc cgtttcgctg ccagcgccgc gatccggctc
      421 taccttcggc tcccggcctt gcgccgcgat gagtcgctcc tgcgcgccgc ccgcctgaac
      481 gttcgcgccg cccgcatctg cccccatccg ggactcctcc gccggctcga acggagcctc
      541 gatgcctccg tcggccgcct cctccgcacg aactttgact gggacgccct ctcgcacgag
      601 tcgcggccgg accaggtggc caagggaatt atcctcaagc accccgtctc cccgcgcgag
      661 aagggcgtgt tgttcatttc attcgagggg cagtggctcc gtctcttccg ccatgccgac
      721 atcgaccgcc tcgcccgcga ctaccacctc gttatcgccc cgtcttggtc cccgccccac
      781 agcctcgcgt tttcggtcgc cgcccgccgg tggcccggac ggttcttcac cctcatcagc
      841 aatctcaatg agatccccgt ctttgcgcgc atcgccccga attccgaggt ggtcccgctt
      901 ttcggctcca gttgggtcca cccgcagatt ttcgacgtct ccgcgccggt cgccaaagag
      961 tacgacatcg tcattctcgc caacttcgcc gcctacaagc gccacttcct tttgttccgc
     1021 gcgctccgcg acatgcgccc cgacacccgg gtgctgctgc tgggcaaatc catggacggc
     1081 cgcacccccg agacgatcat gaacgaggcc cgcgcctacg gcgtcgccga ccgcatcacg
     1141 atcaaaccgg ggctgcccga cggggagatg atccgcgcct ttcgctccgc ccgggtcgcc
     1201 ctgatgctct ccggcaatga aggctcctgc gtcgcggtcg tcgagtcgat gttcgccgac
     1261 attcccatcg gcgtcttcga ggacgccgtg attggctccc tcgcctacgt caacgagcac
     1321 accggccgcc gcctccgccg ccgccgcctc ggccggcagc tcgaggaatt catcgccgac
     1381 tccggccgct actccccgcg ccggtgggtc atggaaaacg gcatctcctg tttcggcagc
     1441 accgccgtgc tcaacgacgc cctccgcgcc cgcgtccttg agtagggcga agagt
//
LOCUS       DAOOKS010000552         1493 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_111241_length_1493_cov_2.915855, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000552 DAOOKS010000000
VERSION     DAOOKS010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1493
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111241_length_1493_cov_2.915855"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1295)
                     /locus_tag="PK112_08420"
     CDS             complement(<1..1295)
                     /locus_tag="PK112_08420"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPC12106.1"
                     /translation="MELLRAEHLSFRYETQSELLLADVSFSLDHAARVGLIGNNGSGK
                     TTLLALIRSELSPVSGSLTVRPDTRTGSLPQEVAFAEDASVEDYLWSARPDLDHLRRR
                     LAAADQSSVDYADLMNLFYQAGGAVTEAAIAHLCAGFRLHPSLLRRPLAVLSGGEKTK
                     VALARLLLLGPDLLLLDEPTNHLEIAALEWLETYLAGLSIPCIVISHDRRFLDVCANE
                     IWELDRGRLTVYSGNYSFYRAAREADRRRRERICEEAQQKAKRLREAAARRRAESERT
                     EHFKFKRSVSKRGSIQKRDAGSGRSGADSSKTMRAALALEKRLAALREQAEAQRPFLE
                     KERRITLACPPLDAPLPLTVANLAHAFGGRSVLSGLSFSLRGGAHLGLIGPNGSGKST
                     LLKILAGRLEPTSGAFHWTPRARLGYYSQEHDILDPAAR"
BASE COUNT          263 a          418 c          574 g          238 t
ORIGIN      
        1 atgcgcgcgg ccgggtcgag aatgtcatgc tcctgtgagt agtagccgag gcgggcgcgc
       61 ggggtccagt gaaaggcgcc ggaagtcggt tcgaggcggc cggcgaggat tttgagcagg
      121 gtcgatttgc ccgagccgtt ggggccgatg agtccgaggt gggcaccgcc gcgaagcgaa
      181 aacgaaaggc cgctcaggac agagcggccg ccgaaagcat gggcgaggtt ggcgaccgtg
      241 aggggaagag gggcgtcaag gggcgggcag gccagcgtga tgcggcgctc cttctcaagg
      301 aacggccgct gggcctccgc ctgttcgcgc agggcggcga ggcgtttctc caaagcgagg
      361 gcggcgcgca tagttttgga cgagtcggcc ccgcttcgcc cagaaccggc atcccgcttc
      421 tgaatgctcc ccctcttact gaccgagcgc ttgaacttaa agtgttcggt gcgttcggat
      481 tcggcgcggc gccgggcagc cgcctcgcga agcctcttcg ctttctgctg cgcttcttcg
      541 cagatccgct cgcggcggcg gcggtcggcc tcgcgcgccg cgcggtaaaa tgagtagttg
      601 ccggagtaga ccgtcaggcg gccgcggtcg agctcccata tctcgttggc gcagacatcg
      661 aggaaacggc gatcgtgact gatgacgatg caggggatgg acaggccggc gaggtacgtc
      721 tcgagccact cgagggcggc gatctcgaga tggttggtcg gttcatccag cagcagcaga
      781 tcgggcccga gcaagaggag acgggcgagc gcgaccttgg tcttctcgcc gcctgagagg
      841 accgccagcg ggcggcggag gagcgacggg tggaggcgaa aaccggcgca caggtgcgcg
      901 atcgcagcct ccgtgaccgc gccgcccgcc tggtaaaaca ggttcatgag gtcggcatag
      961 tcaaccgacg actggtcggc cgcggcgagg cggcggcgga gatggtcaag gtcggggcgg
     1021 gccgaccaga ggtaatcctc gacggaagcg tcttcggcaa aggcgacctc ctggggaagg
     1081 ctgccggtgc gggtgtcggg gcggacggtc aggctgccgg agacggggga aagctcgctg
     1141 cggatgagcg ccagaagggt ggtcttcccg gagccgttgt tgccgatgag gccgacgcgg
     1201 gcggcgtgat cgagggagaa agagacgtcg gcaagcagga gctcggattg cgtctcatag
     1261 cggaaagaga gatgttcggc gcggagcaat tccatagagt ttattgtcgg cgcacgctgg
     1321 tgcgggcatt cgcggccggg tttttccggg tcgcggggca gcctgtcgga acctgaccgg
     1381 cacgactcac aatacgctgc ggcgacgggg ggcgccagtt gacttttcgc gttccccgcg
     1441 ttacccttgc agcaacgtgc aatgaggttc gacctgagga gagaatcatg aag
//
LOCUS       DAOOKS010000553         1491 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_111449_length_1491_cov_3.671309, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000553 DAOOKS010000000
VERSION     DAOOKS010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1491
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111449_length_1491_cov_3.671309"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="PK112_08425"
     CDS             <1..807
                     /locus_tag="PK112_08425"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF014498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase"
                     /protein_id="HPC12107.1"
                     /translation="VFLPNPLAGCPMADMAEMADVLAAWECLEEFGGDRRVMPISYMN
                     TAAGLKAFTGRHGGLICTSSNARAALTWAFGLREKVFFFPDEHLGYNTGIALGIAPEE
                     MVLWDFKRDGGGLTRAQMEKAKIILWKGHCHVHTNFRAEHVEQVRRDYPGVKVVVHPE
                     CPHEVVRLADSVGSTSHIVDFCRKAPAGSTIAIGTEINLIHRMALEHPDKRIFELSGQ
                     TCPVCANMYRTTLNDLVYTLENLDTVKPIAVKEPIRSEARLALEKMLEIA"
     gene            908..>1491
                     /locus_tag="PK112_08430"
     CDS             908..>1491
                     /locus_tag="PK112_08430"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="E3 binding domain-containing protein"
                     /protein_id="HPC12108.1"
                     /translation="MAVDVLVPQMGESVLEGTILEWKVKIGDRVKADQPLVELMTDKI
                     NIEIPSETDGILTHLYAKEGEVVPVGTRIATIDDGGAAAKTAKTSEERPQAAPAAVSA
                     PAAPPKPRPAVAGTAPAGQGKLSPKVRVMIREYSLDPATIVPTGKDGIVKVEDVLRAV
                     EERSLAQGFAAAPIPAPAPPESAAAGPRPETPPPA"
BASE COUNT          281 a          456 c          511 g          243 t
ORIGIN      
        1 gtcttcctcc cgaatccgct ggccgggtgt ccgatggcgg acatggccga gatggcggat
       61 gtgctggcgg cctgggagtg tctcgaggaa ttcggcggcg accgccgggt catgccgatc
      121 tcctacatga acacggcggc cgggctcaag gcctttaccg ggcgccacgg tgggctgatc
      181 tgcacctcgt ccaacgccag ggcggccctc acctgggcct tcgggctgcg ggagaaggtc
      241 ttctttttcc cggacgaaca cctcggctac aacaccggga tcgccctcgg catcgccccc
      301 gaggagatgg tgctgtggga cttcaagcgg gacggcggcg ggctgacccg cgcgcagatg
      361 gagaaggcga agatcatcct ctggaagggg cactgccacg tccacaccaa tttccgcgcc
      421 gaacacgtcg aacaggtgcg gcgcgactac cccggggtga aggtggtcgt gcatcccgag
      481 tgcccccacg aggtggtccg gctggccgac agtgtcgggt cgacctcgca cattgtcgac
      541 ttctgccgca aggcgccagc cgggagcacg atcgcgatcg gcactgagat caacctgatc
      601 caccggatgg ccctggagca tcccgacaag agaatcttcg agctctcggg gcagacctgc
      661 ccggtgtgcg ccaacatgta ccggacgacg ctcaatgacc tggtctacac gctggagaat
      721 ctcgatacgg tcaagccgat tgcggtaaaa gagccgatcc gcagcgaggc tcgcctggcg
      781 ctggaaaaga tgcttgaaat cgcttagcat ttccgcaggg gatccttgac tcttccggta
      841 gattttccgt atattaacag cttggattag aaatcattat atgtttgcca gataaggaaa
      901 tggagcgatg gcagttgatg ttctcgtccc gcagatgggg gaatcggtgc tcgagggaac
      961 tatcctcgaa tggaaggtca aaatcggcga ccgggtgaaa gcggatcagc cgctggtcga
     1021 gctgatgacc gacaagatca atatcgaaat cccgtcggag acggacggga ttctcacgca
     1081 cctgtatgcc aaggaggggg aggtcgtccc ggtgggaacg cgcatcgcca ccattgacga
     1141 cgggggcgcg gcggcgaaga cggccaagac ctccgaggaa cggccccagg cggctccggc
     1201 ggctgtttcc gccccggctg cgccgccgaa gccgcgtccg gccgtggccg ggacggcgcc
     1261 ggcggggcag gggaaactct cgcccaaggt gcgggtgatg atccgggagt actcgctgga
     1321 tccggcgacc atcgtgccga ccggcaagga cgggatcgtc aaggtcgaag atgtgctccg
     1381 ggcagtcgag gagcggagcc tggcccaagg gtttgcggcg gcgccgatcc ctgcgcccgc
     1441 gccgccggag agcgccgcgg ccggcccacg tccggagacg ccgccgccgg c
//
LOCUS       DAOOKS010000554         1491 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_111489_length_1491_cov_2.713788, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000554 DAOOKS010000000
VERSION     DAOOKS010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1491
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111489_length_1491_cov_2.713788"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1491
                     /locus_tag="PK112_08435"
     CDS             <1..>1491
                     /locus_tag="PK112_08435"
                     /inference="COORDINATES: protein motif:HMM:NF012999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPC12109.1"
                     /translation="PGIDRGLAPPVRGTVVLDVTAYLWHHGCGPTAAGMVMGYWSMNG
                     YEQLVKYGVPAMIAGDHGDSVCGAPIGDHYHDYSCPIDGGSPIPDMSELGGAHTSDCI
                     ADFMRTSWSAAYNLYGWSWFVDVGQALLAYPPYADPTYTHFRMRELDFSSVSFTDYKA
                     EIDAGRPTVFLVDTDGNGVTDHFVTGIGYSEDPPKYGVHDTWDQSIHWYLWRAIAPGV
                     SWGIYGMHFFGPAVSFSAAPRFGDISLEVQFTGQSFDPPDSWQWDFGDGQSSSVRSPS
                     HQFTAPGIYDVSLTATFAGEPISSLREDFIIALADSLAGQDQLVEGGSEVEVVISASN
                     TVPLKEILIPLSYAGTLDLTLVSFSTAGCRTDYFESTGYFDFAPGRDQATIYLISSTA
                     GTSPELAGGSGPVVRLTSSTGSAGPGDSAVISMSGYSTYSPRFSGSLLAYTPKTLSPV
                     LRYAECCVGERGDVDGDGSVKISDVTALVSYFFRDGEPPACNDEADS"
BASE COUNT          249 a          436 c          490 g          316 t
ORIGIN      
        1 cccggcatcg atcgcgggct ggcgccgccc gtacggggga cggtggtgct ggacgtaacg
       61 gcgtacctct ggcaccacgg gtgcggcccg acagccgccg gcatggtgat ggggtactgg
      121 tcgatgaacg ggtacgaaca gctggtcaaa tatggcgtgc cggctatgat tgccggcgat
      181 cacggggatt cggtgtgcgg cgctccgatc ggcgaccact accacgatta ctcatgcccg
      241 atcgacggcg ggagtccgat tccggacatg tcggagctcg ggggggcgca tacgagcgac
      301 tgcatcgcgg acttcatgag aacatcctgg agcgccgcct acaacctgta cgggtggagc
      361 tggtttgtgg acgtggggca ggcgctgctg gcttaccctc cgtacgcgga tccgacctac
      421 acgcattttc ggatgcggga gctggatttc tcgagcgtgt cgtttaccga ctacaaggcg
      481 gagatcgacg cggggcgacc gaccgtattc ctggtggaca ccgacggcaa cggggtgacg
      541 gatcacttcg tgaccggaat tggctactcg gaagacccgc cgaagtatgg ggtgcacgac
      601 acgtgggacc agtcgatcca ctggtacttg tggcgtgcca ttgcccccgg cgtgagctgg
      661 ggaatatacg gcatgcattt tttcggcccg gctgtcagtt tctccgctgc cccgcgcttc
      721 ggggatatct cgctcgaggt ccagttcacg gggcaatcgt ttgatccgcc agattcatgg
      781 cagtgggatt tcggcgatgg ccagtcctcc tcggtgcgat cccccagcca tcagttcacc
      841 gccccgggga tttacgatgt ctcgctcacg gcaaccttcg ccggcgagcc gatttcctct
      901 ctgcgcgagg atttcattat tgccttggcc gactcgcttg ccggtcagga tcaattggtc
      961 gagggcggca gcgaagtcga agtcgtgatt tcggcctcga acacggtccc gctcaaggag
     1021 atactgattc ccctgtcgta cgcggggaca ctcgacctga ccctggtgtc gttctcgact
     1081 gcgggttgtc ggacagacta tttcgaatcg accggctatt ttgatttcgc gccgggacgg
     1141 gatcaggcga cgatctacct catcagttca accgcaggta cttcgccgga gcttgcaggc
     1201 gggagtggcc ccgtggtcag gctcacgtct tccaccggct cggcggggcc gggggattcg
     1261 gcggttataa gtatgtccgg gtattcgacg tactctccgc gattctccgg ttctctgctg
     1321 gcctacacgc cgaagacctt atccccggtc ctcaggtatg cggagtgctg cgtaggtgaa
     1381 cgtggcgatg tggacggcga cgggtcagtc aagatttccg atgtcaccgc cctggtctcg
     1441 tacttcttcc gggatggaga accgccggcg tgcaatgacg aggcggacag t
//
LOCUS       DAOOKS010000555         1486 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_112100_length_1486_cov_2.408106, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000555 DAOOKS010000000
VERSION     DAOOKS010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112100_length_1486_cov_2.408106"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            13..168
                     /locus_tag="PK112_08440"
     CDS             13..168
                     /locus_tag="PK112_08440"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HPC12110.1"
                     /translation="MDVDSNTEVARKFGIMSIPSLIFFKNGEELDRVVGAIPKSQLTQ
                     RLEKVLA"
     gene            592..1416
                     /locus_tag="PK112_08445"
     CDS             592..1416
                     /locus_tag="PK112_08445"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPC12111.1"
                     /translation="MRQYQQYDGPARGMSFGPGGVSPWIKWMFIVNGGMYVLQLIFPV
                     ITSYLGLTPARFFREFPIWLHTPLTYMFLHGGFLHVAFNMFALWMFGTEIEQTWGTGA
                     FRRFYLLAGLSGGAATLALNSGQAAVTVGASAAIYGVLVAYWFMFPNRLLYIYFLVPV
                     KVKYAIPGMIVLVFLAGGPSIAHMAHLGGALFAVVYMKTDWRWLRFGRSIKNLRHRRQ
                     EAKLQKNRERAEDIMKRVDAILDKINEVGIDNLTPEERRFLEEASSQLSGKPGEDK"
BASE COUNT          291 a          415 c          484 g          296 t
ORIGIN      
        1 aaggtcgcca agatcgacgt ggattcgaat accgaagtgg cgcgcaagtt cgggatcatg
       61 tcgattccct cgctgatctt ctttaagaac ggcgaggaac tggatcgggt ggtgggggcg
      121 attccgaagt cgcagttgac gcagcggttg gagaaagttc tcgcctagaa ttcgattctc
      181 ggcagtgcag gcacaggcgc cggcggccac tgaagccgtc ggcgtttttg cgcccacccc
      241 tccggcggcc ccgcgcggaa cttttggcgg gggccgccga ttacagaagc agacggcccg
      301 tgccgagagc gcggacaatg gacttgattt ttcccggagg acagcgctag tatatgagtt
      361 ggtacgacat cgcagttctg gcggtttttc tggttgtctg gctggtgctg atcacgcgag
      421 tcctgcccaa attcggcgcg ggctgactgc ccgggcgctg tgatcttccg gtggaagata
      481 aacgggatca acaggataca accgacgagg cggtacggaa agaagcgccc gacagaaatt
      541 gacggatgcg ctcatgatcg gatccggcac ggcaaccgat atctaagacg gatgaggcag
      601 tatcagcaat acgacgggcc ggcgcgcggg atgagttttg gtccgggcgg agtctccccc
      661 tggatcaagt ggatgtttat tgtcaatggg gggatgtacg ttctccaact catcttcccg
      721 gtgatcacct cctacctcgg gctcaccccg gcccggttct tccgcgagtt tccgatctgg
      781 ctgcataccc cgctgaccta catgttcctc cacggcgggt tcctccacgt cgctttcaac
      841 atgttcgcgc tgtggatgtt cgggaccgaa atcgagcaga cgtgggggac cggcgcgttc
      901 cggcgcttct acctgctggc cgggttgtcg ggcggggcgg ccacgctggc gctgaactcc
      961 ggacaggcgg cggtgactgt gggcgcctcg gccgcgatct acggcgtgct cgtggcctac
     1021 tggttcatgt tccccaaccg gctgctgtat atctactttc tggtgccggt caaggtcaag
     1081 tacgccattc ccgggatgat cgtgctcgtg tttctcgccg ggggaccgag catcgcgcac
     1141 atggcgcacc tgggcggtgc gctgttcgcg gtcgtctaca tgaagaccga ctggcggtgg
     1201 ctgcgcttcg ggcgctcaat taaaaacttg cgtcatcgcc gccaggaggc caagcttcaa
     1261 aaaaacaggg aaagggccga ggacataatg aaacgggtcg acgccatcct cgacaaaatc
     1321 aacgaggtcg gcatcgacaa cctcacgccg gaggaacgga ggttcctcga agaggcgtca
     1381 tcgcagctat cgggcaagcc cggggaagac aagtagcgag aatgcgaaaa aaagtattgc
     1441 tggcggaacg gtccgatgcg acccggagtg tggcggagac gatcct
//
LOCUS       DAOOKS010000556         1485 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_112169_length_1485_cov_3.670629, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000556 DAOOKS010000000
VERSION     DAOOKS010000556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112169_length_1485_cov_3.670629"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1245
                     /gene="hutI"
                     /locus_tag="PK112_08450"
     CDS             <1..1245
                     /gene="hutI"
                     /locus_tag="PK112_08450"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HPC12112.1"
                     /translation="TLLVKNAGQVITMAGPVPRLGPALRDIGLIAHGAIAAAGDEIVA
                     VGPSDDIERHTRLAENCTVINAKGAVVTPGLIDPHTHPVFSMTREKEFEMRIEGKTYM
                     EIAAAGGGIRASVRDLRSADRAVLLEQTRRRVARMLRYGMTTIEAKSGYGLSTESELR
                     QLEIIREVDQQCPIDLIPTFLGAHEVPDEYRERRSEYVDLLVGEMIPAVAAAKLAEFS
                     DIFTEVGVFEIEDSRRIQQAAKEAGLKLKFHADEIKPLGGAELAAAMGAVSADHLVHV
                     SERGIKALAAAGTVAVLLPGTSFSLASSYAPARKMIEGGVTVALSTDCNPGSSCTESL
                     PFIVSLAALQLKMTAAEALAAVTVNAACAVDRAATLGRLVPGLRADLVIWDMNDYREL
                     PYHYAVNLVSRVIKNGKVVVSQ"
     gene            1272..>1485
                     /locus_tag="PK112_08455"
     CDS             1272..>1485
                     /locus_tag="PK112_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12113.1"
                     /translation="MHRQAYRSVAAALFLTGLGLSCGVYTFNPAGKSSIKTIAVSRFE
                     NQTVELALTDQITDGVIDAFIADGTLA"
BASE COUNT          255 a          523 c          463 g          244 t
ORIGIN      
        1 acccttttgg tcaaaaacgc cggccaggtg atcacgatgg ccggcccggt cccccgcctc
       61 ggccccgctc tccgcgacat cgggctgatc gcgcacggcg cgatcgccgc cgccggcgat
      121 gagatcgtcg ccgtcggccc atccgacgac atcgaacgcc acacccggct cgccgaaaac
      181 tgcaccgtca tcaacgccaa aggggcggtc gtcacccccg gcctcatcga tccccacacc
      241 cacccggtct tctccatgac gcgcgagaag gagttcgaaa tgcgcatcga ggggaagacc
      301 tacatggaga tcgccgcggc cggcggcggc atccgcgcct ccgtccgcga cctccgctcc
      361 gccgaccgcg cggtcctgct cgaacagacg cggaggcgtg tcgcccggat gctccgctac
      421 ggcatgacaa cgattgaggc caagtccggg tacggccttt cgaccgagtc cgagctccgc
      481 cagctcgaga ttatccgcga ggtcgaccag cagtgcccga tcgatctgat accgacgttc
      541 ctcggcgcgc acgaagtccc cgatgaatac cgcgagcgtc ggagcgagta tgtggacctg
      601 ctcgtcggcg aaatgatccc ggcggtggcc gcggccaaac tggccgaatt ctccgacatc
      661 ttcaccgagg tcggcgtgtt cgagatcgag gattcgcgcc gcattcagca ggccgccaag
      721 gaggccgggc tgaagctgaa attccacgcc gatgagatca agccgctggg cggcgccgag
      781 ctggccgccg ccatgggcgc ggtctcggcc gaccacctcg tccatgtatc cgaacggggg
      841 atcaaggccc tggccgccgc cggcacggtg gcggtcctgc tgcccggaac ttccttctcc
      901 ctcgcctcgt cctatgcccc ggcgcggaag atgatcgagg gcggcgtgac ggtcgcactc
      961 tcgaccgact gcaacccggg ttcaagctgc accgagtcgc tccccttcat cgtgtcgctg
     1021 gcggcgctcc agttgaagat gacggccgcc gaggcgctgg ccgcggtgac ggtcaacgcc
     1081 gcctgcgccg tcgaccgggc cgcgactctc ggccgcctcg tccctggcct gcgcgccgat
     1141 ctggtgatct gggacatgaa cgactaccgg gagcttcctt accactatgc ggtcaatctc
     1201 gtctcccggg tgatcaagaa cggaaaagtg gtcgtgagcc aatgagcggc ccgcgcgagg
     1261 agagaacgat catgcatcgc caggcgtatc ggtcggttgc cgcggccctg tttctcacag
     1321 gcttggggct ttcctgcggc gtgtacacat ttaacccggc ggggaaatct tcgatcaaaa
     1381 ccatcgccgt ctcccggttt gaaaaccaga cggtcgagct cgcgctgacc gatcagatca
     1441 ccgatggcgt catcgacgcc ttcattgccg acggcacgct ggcgg
//
LOCUS       DAOOKS010000557         1483 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_112459_length_1483_cov_2.801120, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000557 DAOOKS010000000
VERSION     DAOOKS010000557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112459_length_1483_cov_2.801120"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /gene="rplU"
                     /locus_tag="PK112_08460"
     CDS             <1..303
                     /gene="rplU"
                     /locus_tag="PK112_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009852294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HPC12114.1"
                     /translation="VAGFQYSAQEGDVLRVPHQKADPGTSLAIDRILLVRGDGATAIG
                     TPTVADARIDAEVVGEGLADKVLIYKYKRRTKYRRTQGHRQRFTEIRINKIHAPGA"
     gene            370..>1483
                     /locus_tag="PK112_08465"
     CDS             370..>1483
                     /locus_tag="PK112_08465"
                     /inference="COORDINATES: protein motif:HMM:NF018899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HPC12115.1"
                     /translation="MLSLSAHAQNTAEQFRVVDIQVEGNRVATRSLILGVASLEVGAV
                     LSVTDVSESIRRLYALGIFRDVRIEAEPVTGGIRIFIIVEELPKLAGLAFEGNKKLKD
                     DKLRKEIGLGVGGYISPYLIEKSRAKIFDLFAAEGYFRAKIEPELIYGADSVEAVLQF
                     NIRERDKVKVEQVVVTGSRRVEAGDLVGRMRNRKRGLLRTSDFAQDKYDEDLQKVIDE
                     YHKRGFIDAYLINDSLAIDTARNRMTIYLDVYEGPRYYFGETSFKGNERLSADHLAKL
                     LKHKAGQVFDSEKYDASLGEVASGYYDIGHLHVEIFDERSTRSDSLIDITYDITEGLP
                     SNINLVKIVGNYKTKDRVIRRELSTLPGQVFDRTALI"
BASE COUNT          292 a          471 c          462 g          258 t
ORIGIN      
        1 gtcgctggtt ttcagtatag cgctcaggaa ggggatgtcc tccgggtacc ccaccagaaa
       61 gccgaccccg gcacgtcgct ggccatcgac cgcatcctct tagtccgcgg cgacggggcc
      121 acggcgatcg gtacgccgac ggtcgccgat gcgcgcatcg atgcggaagt tgtgggtgaa
      181 ggcctggccg acaaggtgct catctacaaa tataagcgcc ggaccaagta ccgccgcacc
      241 caggggcatc gccagcgttt caccgagatt cgcatcaaca aaatccacgc ccccggcgcc
      301 tgagcgcagt tgagtcggac gaacggaccc cgagcaggga ttgctcgcgc ctgtctcttg
      361 gcggcgctgg tgctgagcct gtcggctcac gcccagaata ccgcggagca gtttcgggtg
      421 gtcgatatcc aggttgaggg caaccgggtg gcgacccggt cgctcatcct gggcgtcgcc
      481 tctcttgagg ttggcgcggt tctttcggtc accgacgtct ccgagtcgat ccgccgcctc
      541 tacgccctcg gcatcttccg cgatgtccgc atcgaggccg agccggtgac cggcggcatc
      601 cggattttca tcattgtcga agaactcccc aagctggccg ggttggcatt cgaggggaac
      661 aagaagctca aggacgacaa actccgcaag gagatcggcc tgggcgtggg cggctacatt
      721 tccccctacc tgatcgagaa gagccgggcg aagattttcg acctgttcgc cgccgaggga
      781 tatttccggg ccaagatcga gccggagttg atctacggcg ccgattccgt cgaggcggtc
      841 ctgcagttca acattcgcga gcgcgacaag gtcaaagtcg agcaggtcgt ggtcacgggc
      901 agccggcggg tggaggccgg cgacctggtc ggcaggatgc gcaaccgcaa gcgcggtctc
      961 ctgcgcacct ccgacttcgc ccaggacaag tacgacgagg acctgcagaa agtcatcgac
     1021 gaataccaca agcgcggttt catcgacgcc tacctcatca acgattcgct ggcgatcgac
     1081 acggcccgca accggatgac catctacctc gacgtctacg aaggcccccg ttactatttc
     1141 ggcgagacgt cgttcaaggg aaacgagagg ctctcggcgg accatctcgc gaagctgctg
     1201 aagcacaaag cggggcaggt gttcgactcc gagaagtacg acgcctcgct cggggaggtg
     1261 gcctccggct actacgatat cggccacctc cacgtggaga ttttcgacga gcggtcgacc
     1321 cggtccgact cgctcatcga tatcacctac gacatcaccg aggggctccc ctccaacatc
     1381 aacctcgtga agatcgtcgg gaactacaaa accaaggacc gggtgatccg gcgggagctg
     1441 tcgacgctgc cgggccaggt gtttgaccgg acggcgctga tcc
//
LOCUS       DAOOKS010000558         1477 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_113162_length_1477_cov_2.077356, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000558 DAOOKS010000000
VERSION     DAOOKS010000558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113162_length_1477_cov_2.077356"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            332..>1477
                     /gene="hisS"
                     /locus_tag="PK112_08470"
     CDS             332..>1477
                     /gene="hisS"
                     /locus_tag="PK112_08470"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HPC12116.1"
                     /translation="MAKDDRKKPEKVKPQVLKGFRDYPPDEQIAREKMLARCREAVEL
                     MGFLPLQTASLEFADTLLGPHYSAENLAELFGFRGPDDVDMALRYEFTVSLARYVAGN
                     PALPLPFRRYQYGNVWRVDKPGPGRFREFMQFDIDIVGTRNLLADAEIIAAMVKTLDH
                     LGIGKFKVRYSTRRLLNGLIEFAGIPAAVGPDVMRVIDKLEKQGRAAVVLELGPGRTD
                     ASGDRIPGLGLADGQIAQIEKFLEIAGQHDRDHLPEAERLLGGIPASREGLDELKEIC
                     DHLAAMGVDPAKSGVDLTIVRGLGYYTGPVFETTLLDLPEYGSVFSGGRYDNLVERFI
                     NQSVPGVGSSIGIDRLLAALLKLETLDPAQATSQVIVTVMDREHIPDY"
BASE COUNT          289 a          498 c          432 g          258 t
ORIGIN      
        1 tgaaacgaac accggcggct cgagttcatc gggcggaaca gccaggctcg acgcagccag
       61 gagaccggcc gccgacagca gtggtagcaa gacaactaat cgaaagaaca tgcgcccttc
      121 ccctcgttca tagttccgtg atgttcgtgc tatcatcgac aaacatacct aatttccgag
      181 aaatgtcaat gcaaatacac cgatactact gccgccggat ggtcaaccgg agaccgatag
      241 ggaaggaaaa aatgcttttc tttgggtcgc gattgcctac atttacccgc gagagtcggt
      301 gaccggaagc ccagcaacaa gttaggagac catggccaaa gacgaccgca agaagccgga
      361 aaaggtaaag ccgcaggtgc tcaagggatt tcgcgactac ccgcccgatg agcagattgc
      421 ccgcgagaag atgctcgcca ggtgccgcga ggcggttgag ctgatggggt ttttgcctct
      481 ccagaccgcg tcgctggagt tcgccgacac gctgctcggg ccccactaca gcgcggagaa
      541 cctcgccgag ctgttcggtt ttcgcggtcc cgatgacgtc gacatggctc ttcgctacga
      601 gttcacggtc tcgctggccc gctatgtggc cggcaacccg gcgctgcccc tgccgttccg
      661 ccgctaccag tacggcaacg tgtggcgggt ggacaagccg ggcccggggc ggtttcgcga
      721 gttcatgcag ttcgacatcg acatcgtcgg cacgcgaaac ctcctggccg acgcggagat
      781 catcgccgct atggtgaaga ccctcgacca tctgggcatc ggcaagttca aggtccgcta
      841 ctcgacccgc cggcttctca acggcctgat tgaattcgcc ggcatccccg ccgccgtcgg
      901 ccccgacgtc atgcgcgtga tcgataagct cgaaaaacag gggagagcgg cggtggtgct
      961 cgagctcggt cccggccgga ccgatgcctc cggcgaccga atccccggtc tcggcctcgc
     1021 cgacggccag atcgcccaga ttgagaagtt cctcgagatc gccgggcagc acgaccgcga
     1081 ccatcttccc gaggccgaac gcctgctggg cggcatcccc gcctcccgcg agggtctcga
     1141 cgagctcaag gagatttgcg accacctggc cgccatgggt gtcgatcccg ccaaatccgg
     1201 cgtcgacctg acgatcgtcc gcggcctcgg ctactacacc ggccccgtct ttgaaaccac
     1261 cctcctcgac ctccccgaat acggctcggt cttttccggc ggccgctatg acaacctcgt
     1321 ggagcggttc atcaaccaga gcgtccctgg tgtggggtcc tcgatcggca tcgaccgcct
     1381 cctcgccgcg ctcctcaagc ttgagaccct cgacccggcg caggccacct cgcaggtgat
     1441 cgtcaccgtc atggaccgcg agcacatccc cgactat
//
LOCUS       DAOOKS010000559         1476 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_113224_length_1476_cov_3.218156, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000559 DAOOKS010000000
VERSION     DAOOKS010000559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113224_length_1476_cov_3.218156"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            69..>1476
                     /locus_tag="PK112_08475"
     CDS             69..>1476
                     /locus_tag="PK112_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005009081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HPC12117.1"
                     /translation="MASDERATSNFIRDIIDDDLAKGTYDGRVHTRFPPEPNGYLHIG
                     HAKSILLNYGLAQTYGGKFNLRFDDTNPTKEEEEYVNSIIEDVRWLGADWEDRLFFAS
                     DYFPQLYEWAVQLIKAGKAYVDDLTPEEIRATRGTLTEPGQESPFRNRPIEENLDLFE
                     RMKNGEFPNGAKTLRAKIDMGAGNVNLRDPVMYRVLHARHHRTGDAWCIYPMYDWAHG
                     QSDSVEGITHSICTLEFEDHRPLYNWFLDQLGIYHPRQIEFARLDLTYTVMSKRKLLR
                     LVQEKYVDGWDDPRMPTISGLRRRGYTPESLRDFAERVGVAKKKGSVADLALLEHCLR
                     DDLNKRARRVMAVLDPLKVVITNYPEDRVEEIEAVNNPENAAEGTRMVPFAREIYIER
                     DDFMEHPPAKFFRLSPGAEVRLKHAYFIRCQEAVKDAAGKIVELRCTYDPASRGGEAP
                     DGRKVKGTLHWVSAAHAFE"
BASE COUNT          291 a          446 c          502 g          237 t
ORIGIN      
        1 gaggcgttca gcccgaccct gcgcgccgcc gataaagagt gatacgagaa gattgatgaa
       61 gggtgcctat ggccagcgac gaacgagcga catcgaactt cattcgcgac attatcgatg
      121 atgaccttgc gaagggaacc tacgacggac gggtgcacac ccgctttcct ccggagccga
      181 acgggtacct ccatatcggg cacgccaagt cgattctcct gaactacggc ctggcccaga
      241 cctacggcgg gaagttcaac ctccgcttcg acgacacgaa tccgactaaa gaggaggagg
      301 agtacgtcaa ctccatcatc gaggatgtcc ggtggctcgg agccgactgg gaggaccgtc
      361 tctttttcgc ctcggactat ttcccgcagc tctacgagtg ggcggtgcag ttgatcaagg
      421 cggggaaagc ctatgtcgat gatctgacgc ccgaggagat ccgcgcgacg cgggggacgc
      481 tgaccgagcc gggccaggag tcgcccttcc gcaaccggcc gatcgaggag aatctggatc
      541 tgttcgagcg gatgaagaac ggggagtttc ccaacggggc gaagacgctg cgggcgaaga
      601 tcgacatggg agcggggaat gtcaacctgc gcgacccggt catgtaccgg gtgctgcacg
      661 cccggcacca ccgcaccggc gacgcctggt gcatctaccc catgtacgac tgggcgcacg
      721 gtcagtcgga ctcggtcgag gggatcacgc actcgatctg cacgctcgag ttcgaggacc
      781 accgcccgct gtacaactgg tttctcgacc agctggggat ctaccacccg cggcagatcg
      841 agttcgcccg cctcgatctc acctacacgg tgatgagcaa gcggaagctg ctgcggctgg
      901 tgcaggagaa gtatgtcgac gggtgggacg atccgcggat gcccacgatt tccggcctgc
      961 ggcggcgggg gtacacgccg gagtcgctgc gcgatttcgc cgagcgggtg ggcgtggcca
     1021 agaagaaagg ctcggtggcc gacctcgcgc ttctggagca ctgcctgcgc gacgacctca
     1081 acaagcgggc gcggcgggtg atggcggtgc ttgatccgct caaggtcgtg atcacgaact
     1141 acccggagga ccgggtcgag gagatcgagg cggtcaacaa tccggaaaat gcggccgagg
     1201 gaacccgcat ggtgccgttc gcccgggaaa tctatatcga gcgcgatgac ttcatggagc
     1261 acccgcccgc gaagtttttc cgcctctccc ccggcgccga agtgcgcctg aagcacgcct
     1321 acttcatccg gtgccaggag gcggtcaagg atgcggccgg gaagattgtg gagctccgct
     1381 gcacgtacga cccggcctcg cgggggggcg aggcgccgga cggccgcaag gtgaagggga
     1441 cgctccactg ggtgtcggcc gcgcacgcgt tcgaag
//
LOCUS       DAOOKS010000560         1469 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_114071_length_1469_cov_4.012730, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000560 DAOOKS010000000
VERSION     DAOOKS010000560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114071_length_1469_cov_4.012730"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="PK112_08480"
     CDS             <1..454
                     /locus_tag="PK112_08480"
                     /inference="COORDINATES: protein motif:HMM:NF014874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HPC12118.1"
                     /translation="KQAVYQGMARLEWPGRFQIVARRGRPTLVFDVGHNAGGIAAFVE
                     TFQTRFPGRKALILTGFVKRKEHQKMVDHLAKIAAQYVIVPLDTKRSVDTAHLLGTIN
                     WRRVPVHRAGSVATGWRRVARTAQPDDIIAVIGSHYLVGEFFEKIGSA"
     gene            460..>1469
                     /locus_tag="PK112_08485"
     CDS             460..>1469
                     /locus_tag="PK112_08485"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HPC12119.1"
                     /translation="MDSKERRRPAPETADPERLPQGEALPAGPGPAAARAAAAAVELP
                     AEKLTRLTEANRQLKRKIFDLYTIFEISRNFSSVLNYQTLLDSFVLTCVAQVSASKGA
                     IYLKQDARSDRFSLAKSKGGGAFPPKEATFPSDSKIADYLARLNRPVPTSELLAGFSR
                     EPEAEALRSFDPGLVVPLIYQSRLIGLVLIGEKIGAKEFGLDDIEFLSILGNQISVAI
                     ENARLYESERAATQQLLAAQQQLVHTERLAALGEMSARVAHEVNNPLGIIKNYLLLVQ
                     RTARGNVETGNYLEIISQEIDRIARIVRELLDFHRPEKTALRPMKLGKVIDDVLVLMD
                     RQ"
BASE COUNT          290 a          558 c          387 g          234 t
ORIGIN      
        1 caagcaggcc gtgtaccaag gcatggcccg gttggagtgg cccggccgat tccagatcgt
       61 cgcccgccgc ggccgaccaa ccctggtatt cgacgtcggc cacaacgccg gcggcatcgc
      121 cgcctttgtc gagaccttcc agacccgctt ccccggccgc aaggccctca tcctcaccgg
      181 cttcgtcaaa cgaaaagagc accagaaaat ggtcgatcac ctcgccaaaa tcgccgcaca
      241 atacgtcatc gtacccctcg ataccaaacg ctccgtcgat actgcccacc tcttggggac
      301 catcaactgg cggcgcgtgc cggtccaccg cgccgggtcg gtggcgaccg gctggcgaag
      361 ggtcgcccgg accgctcaac ctgacgatat catagctgtg atcggctcgc actaccttgt
      421 cggagagttt ttcgagaaga tcggctcggc atgatggaga tggattcaaa agagcgccgc
      481 cggccggcgc cggagacggc cgatcccgaa cgcctccctc aaggggaggc gctccccgcg
      541 ggtcccggac cggcggccgc ccgcgccgcg gccgcggccg tcgaactgcc cgccgaaaaa
      601 ctcacccgcc tcaccgaggc caaccgccag ctcaaacgca aaatcttcga cctctacacc
      661 atcttcgaaa tcagccgcaa cttcagctcg gtcctcaatt accagaccct cctcgattcc
      721 ttcgtcctca cctgcgtggc ccaggtctcc gcctccaagg gagccatcta cctcaagcag
      781 gacgcccgct cggaccgctt ttcgctcgcc aagagcaagg ggggaggcgc cttccccccc
      841 aaagaggcaa ccttcccgtc cgactccaaa atagccgact acctcgcccg cctgaaccgc
      901 cccgtgccga cttccgagct gctcgccggc ttctcccggg agccggaggc cgaggctctc
      961 cgtagtttcg atcccggcct ggtcgtcccg ctgatctacc agagccgcct catcggactg
     1021 gtcctcatcg gcgagaaaat aggcgcgaaa gagttcggcc tcgacgacat cgaatttctg
     1081 tccatcctcg gcaaccagat ctcggtggct atcgagaacg cccggctcta cgaatccgaa
     1141 cgcgccgcca cccagcagtt gctggctgcc cagcagcagc tcgtgcacac cgagcgcctc
     1201 gccgctctcg gcgaaatgtc cgcccgggtc gcccacgagg tcaacaaccc gctcggcatc
     1261 atcaagaact acctcctgct ggtccagcgc actgcccgcg gcaacgtcga aaccggcaac
     1321 tacctcgaaa tcatcagcca ggaaatcgac cgcatcgccc gtatcgtccg cgaactcctc
     1381 gacttccacc gccccgaaaa aaccgccctc cgacccatga aactggggaa ggtgatcgac
     1441 gacgtcctcg tcctcatgga ccggcagtt
//
LOCUS       DAOOKS010000561         1462 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_114943_length_1462_cov_3.725657, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000561 DAOOKS010000000
VERSION     DAOOKS010000561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1462
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114943_length_1462_cov_3.725657"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            335..1192
                     /locus_tag="PK112_08490"
     CDS             335..1192
                     /locus_tag="PK112_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12120.1"
                     /translation="MKNQDVKVCAAMSGGRWPAVSAILFLLAAAPALAESPESVAGGP
                     PRTDSPALIGDPQSAPAENVYYINFDSNLEGWYFLNHPDTVQEYYPDQYTCDELTRMR
                     ANGASFALSWGYFDGSGAMMVTCPFRGWYRDSCYYSWRIKSVQVNVPDEWDGLYRVVV
                     RVYKGTGSRIWAKIGYRPAGSANTYYLPGWSELPTEGWTTLTLSEPSQGAFADLDAVS
                     ILFGAYSSDGNVYVDWVQAYEDQPDVPVLVAPQTIRLSRPTFPHFNGPATATTTRSRW
                     LPTPSSPTC"
     gene            1159..>1462
                     /locus_tag="PK112_08495"
     CDS             1159..>1462
                     /locus_tag="PK112_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12121.1"
                     /translation="MATDPKFTNVLIDTSYIYSTSCKVTRPLPFSDYDYLPGGGGAHD
                     PHYYWRVKAHRVGPSGVSLYSNYFQFIINGPHEVPSEYPSILAARGALPPYIGGTVL"
BASE COUNT          282 a          465 c          441 g          274 t
ORIGIN      
        1 gctcggacca agcagcggcg cctcaacggc ttagcgggca gtccgcggtc ggcccggcgg
       61 gcaaccgccc cggcatgtca tccgtagacc tccgctgtcc agattctgct cgccgtgggc
      121 gcccccacga ttgtcggaag gatcctgttc agaaaaagca cagtcggtcc ggccggtctc
      181 ccgatccggt ccatttcatg ggccgcgggg atcccggtga gagattaccg attgacgaaa
      241 gacggttcgc ggttatattg aaccaacagc aggtggtcct ttgtcctgag tcgaacttga
      301 tgcagaacga gaattcttcc cggcggtgga tactatgaaa aatcaagacg tgaaggtctg
      361 tgcggcgatg agcggcgggc gatggccggc cgtgagcgcc atcctgtttc tcctggcggc
      421 ggctccggcc ctcgcggaat cgccggaatc ggtcgccgga ggaccgcccc gaacggacag
      481 cccggctctc atcggcgatc cgcaatcggc cccagccgag aacgtctact atataaattt
      541 cgactccaac ctggagggct ggtatttcct caaccacccg gacacggtgc aggagtacta
      601 ccccgatcaa tacacctgcg atgaactcac tcggatgcgc gcgaacggcg cgagttttgc
      661 cctctcctgg ggctacttcg acgggagcgg cgccatgatg gtcacgtgcc cgtttcgcgg
      721 ctggtacagg gacagctgct actactcgtg gcgtattaag tcggtccagg tcaatgtccc
      781 ggacgagtgg gacggcctct accgggtggt ggtgcgggtc tacaagggaa ccggctcccg
      841 gatatgggcg aaaatagggt atcgaccggc cggcagcgcc aatacgtact acctgccggg
      901 ctggagcgag ctccccacgg aaggctggac gactctcacc ctcagtgaac catcccaggg
      961 agcttttgcc gacctcgacg ccgtgtcgat tctgtttggc gcctactcgt ccgatggcaa
     1021 cgtctacgtg gactgggtgc aggcgtacga ggatcagccg gacgtgccgg tgctggtcgc
     1081 tccgcaaacg atcagactat ccaggccaac cttcccacat ttcaatggac cggcaacggc
     1141 gactactaca cgctccaggt ggctaccgac cccaagttca ccaacgtgct gatcgacact
     1201 tcatacatct acagcaccag ctgcaaagtg acgcggccgc tgccattctc ggactacgac
     1261 tacctgcccg ggggaggggg cgcccacgac ccgcactact actggcgggt caaagcgcac
     1321 cgggtcggac cgagcggggt cagcctgtac tccaattact tccagttcat tatcaatggt
     1381 ccacatgaag taccctcgga gtacccttcg atcctggcgg cccggggcgc cctgcctccg
     1441 tatatcgggg ggaccgttct cg
//
LOCUS       DAOOKS010000562         1460 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_115172_length_1460_cov_3.793594, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000562 DAOOKS010000000
VERSION     DAOOKS010000562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115172_length_1460_cov_3.793594"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="PK112_08500"
     CDS             <1..525
                     /locus_tag="PK112_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HPC12122.1"
                     /translation="NRRVLLLNQNYEPLSVCSAKRAIVLVIEGKAEMIESADGLKLRT
                     VRTSYSLPSVVRLWNFRKVPLKRIMLTRKNIILRDDGRCQYCGANKGPMTVDHVIPRT
                     EGGSDSWSNLVCACERCNNLKGNRTPEQAGMGLLRRPTRPSYITFIQRNYGVADQWRP
                     YLFLDYNNGTTYTT"
     gene            553..1047
                     /locus_tag="PK112_08505"
     CDS             553..1047
                     /locus_tag="PK112_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12123.1"
                     /translation="MPDLLWLTDIHLDFLDTDGRRRFFRRIGESCADGVVVTGDIGEA
                     DTVEGHLAELAEAAAHLSEALPGALARYSRLLVLTHVPPFREACRYAGRIADGDHLPH
                     FSSQVVGEVLEAHLRANPSRFATVLCGHTHGGGKAEVLPNLTVFTGAADYGRPRIQKV
                     LHLS"
     gene            1159..>1460
                     /locus_tag="PK112_08510"
     CDS             1159..>1460
                     /locus_tag="PK112_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12124.1"
                     /translation="MTHPQGPPPYPTQAYTAGYGDAAGSCHTYAAPPPPGYAQPLSAR
                     GRAVATVYALGVTLLFHLISIPLLLWENSLVQRYSMAIWDGGIAEGEFGAWGLVTFG"
BASE COUNT          246 a          510 c          451 g          253 t
ORIGIN      
        1 aaccggcgcg ttctgctttt gaaccagaac tacgaaccgc tctcggtgtg ttcggccaag
       61 cgggcgatcg tcctggtcat cgagggtaaa gccgagatga tcgagtcggc cgacggtctc
      121 aagctgcgca ccgtgcggac ctcctactcc ctgcccagcg tggttcgcct ctggaacttc
      181 cgcaaagtcc ccctcaaacg gatcatgctc acgcggaaaa acatcattct ccgcgacgac
      241 ggccggtgcc agtactgcgg cgccaacaag gggccgatga cggtcgacca cgtcattccc
      301 cggaccgagg ggggcagcga ctcctggtcc aaccttgtgt gcgcctgtga gcggtgcaac
      361 aacctgaaag gcaatcggac gccggagcaa gccgggatgg ggctgctgcg aagaccgacc
      421 cgcccgagtt acatcacgtt catccagcgc aactacgggg tcgccgacca gtggcggcct
      481 tacctgttcc tcgactacaa caacggaacg acatacacaa cgtaggggga ccggcagggc
      541 agaggtggcg cagtgcctga tcttctctgg ctcaccgaca tccatctcga cttccttgat
      601 accgacggcc gacggcggtt cttccgccgg attggggaga gctgcgccga cggcgtggtg
      661 gtcacgggcg acatcgggga ggcggatacc gtcgaggggc acctcgccga gctcgcggag
      721 gcggccgcgc acctgtccga ggctctgccg ggagccctcg cccggtactc tcgtctgctc
      781 gtgttgaccc atgtgccgcc tttccgcgag gcctgccggt acgccggacg catcgcggat
      841 ggcgatcatc tgccgcactt ctcttcgcaa gttgtcggcg aggttctgga ggcgcacctg
      901 cgggccaacc cctcccggtt cgcgaccgtg ctctgcgggc acactcacgg cggcgggaag
      961 gcggaggtgc ttccgaatct cacggtgttt acgggagcgg ccgactacgg ccgtccgcgg
     1021 atccagaagg ttcttcatct ctcctagctc cccgctgccc tctttctgca cattcgcccg
     1081 gaccccgcca aaatcgccgc gccgaccgtc cgataattcg ggaaaacagc attccttttc
     1141 acgattgagg atagaggtat gacgcacccg caaggccccc ccccttaccc gacccaggcc
     1201 tacaccgccg gatacggcga cgcggccggg agctgccaca cctacgccgc cccgccgccg
     1261 cccggctacg cccagccgct ctcggcccgc gggcgagccg tcgcgaccgt ctacgccctc
     1321 ggcgtcactc tcctgttcca cctgatctcg atcccgctcc tgctctggga gaacagcctg
     1381 gtccagcgct acagcatggc gatctgggac ggcggcattg cggaggggga attcggcgcc
     1441 tgggggctcg tgactttcgg
//
LOCUS       DAOOKS010000563         1460 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_115199_length_1460_cov_2.809253, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000563 DAOOKS010000000
VERSION     DAOOKS010000563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115199_length_1460_cov_2.809253"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(139..>1460)
                     /gene="tolB"
                     /locus_tag="PK112_08515"
     CDS             complement(139..>1460)
                     /gene="tolB"
                     /locus_tag="PK112_08515"
                     /inference="COORDINATES: protein motif:HMM:TIGR02800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HPC12125.1"
                     /translation="SFPTILAAALLSFASSARSQSGESVRQIMFDTIQARAVEPLKVG
                     VDQMKYIGIQYITAEDSSLMLHATAVVQRDIDFYADFDLVPLDTFYLRVYEITEVDLL
                     GWERLGAERLVRLEAEFPGSNIRIYWRLYDVTHNQEIAKGQVEYHRAYWRELSHDVAN
                     EVVYRLTGDPGIFRTKIAYVKQLGKDLKEIFIADYDGANERQLTKTGSICLSPAFTPD
                     GREIYFTSFLGGKADLYKVGVDDGKVVKIAAYEGINSAPAVAPDGRKIACVLSLDGDA
                     EIYTLDIGGRIMSRLTRLRSIESAPSWSPDGRRIVFSSDRTGQPQLYVMNDDGLNERR
                     LTFRGSYNDSPVWAPQGDRVVFVTRTDAGFNIASVDVGGSEYHMLTNLGNNENPHFAP
                     DGKHVIFTSSRLGGTDLYTMDAGGRNQRRLTQTKNVSNPAWGPFPRR"
BASE COUNT          251 a          395 c          543 g          271 t
ORIGIN      
        1 agtcactttg cacgcaatct atccgtttgc cgaaaaacgg tcaacataaa acgaaaaaaa
       61 agcattcccg gggtcgtgtc gcaaattaca ccgcgcgcgc agccgatggt tctcgcgcgc
      121 gggccgggcg ggggccgctc accgccgcgg gaagggcccc caggcggggt tgctgacgtt
      181 tttggtctgg gtaagccgcc gctggttgcg gccgccggcg tccatcgtgt acaggtcggt
      241 gccgccgagg cgcgaggagg tgaatatcac gtgcttgcca tccggggcaa agtgggggtt
      301 ttcgttgttg ccgaggttgg tcagcatgtg gtactcggag ccgccgacat cgaccgaagc
      361 gatgttgaac ccggcatcgg tgcgggtgac gaagacgacg cggtcgccct ggggcgccca
      421 gacgggggag tcgttgtagc tgccgcggaa ggtgaggcga cgctcgttca ggccgtcgtc
      481 gttcatcacg tacaactggg gctggccggt gcggtcggag gagaagacaa tccgccggcc
      541 atcgggcgac cagctcggag ccgattcgat cgagcggagg cgggtgaggc ggctcatgat
      601 ccgcccgccg atatcaaggg tgtagatctc agcgtcgccg tcgagcgaca gcacgcacgc
      661 aatcttgcgg ccgtcgggag ccaccgccgg cgcggagttg atcccctcgt aggctgcgat
      721 tttcacgacc ttgccgtcgt cgactcccac cttgtacaga tcggccttgc ccccgaggaa
      781 cgaggtgaag tagatctctc tcccgtcggg cgtgaaagcc ggggagaggc agatggagcc
      841 ggttttggtg agctggcgct cgttggcgcc gtcgtagtcg gcgatgaaga tttccttgag
      901 gtctttgccg agctgtttga cgtaggcgat cttggtgcgg aaaatgccgg ggtcgccggt
      961 gaggcggtag acgacctcat tggcgacgtc gtgagacagc tcgcgccagt aggcgcggtg
     1021 gtattcgacc tggcctttgg ctatctcctg gttgtgcgtg acgtcgtaga ggcgccagta
     1081 gatgcggatg ttggagccgg ggaattcggc ctcgaggcgg acgaggcgtt cggcgcccag
     1141 gcgctcccac ccgaggaggt cgacctcggt gatctcgtac acgcggaggt agaaggtgtc
     1201 gagggggacg aggtcgaagt cggcgtagaa gtcgatgtcg cgctggacga ccgcggtggc
     1261 gtggagcatg agcgaggagt cctcggcggt gatgtactgg atcccgatgt atttcatctg
     1321 gtcgacgccg accttgaggg gttcgacggc gcgggcctgg atcgtgtcga acatgatctg
     1381 gcggacggat tcgccggact ggctgcgggc ggaactcgcg aacgagagga gtgcggcggc
     1441 gaggatggtc ggaaacgaca
//
LOCUS       DAOOKS010000564         1457 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_115589_length_1457_cov_2.524964, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000564 DAOOKS010000000
VERSION     DAOOKS010000564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115589_length_1457_cov_2.524964"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="PK112_08520"
     CDS             complement(<1..889)
                     /locus_tag="PK112_08520"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPC12126.1"
                     /translation="MAMQVGVIGCGYWGPNLIRNIAAHPEMKVQAIADLRRERLNLIA
                     RQYPTITRCSADAGEVIRAPEIDAVVIATPVSSHFALAMDALEAGKHVFLEKPFTATV
                     EQAERLIETAERKNLRLFVDHTFLYTGAVQTIKRLVDGGELGELYYFDSVRVNLGLFQ
                     HDVNVIWDLAAHDVSIMDYLLGRDPRAISATGVAHFDRDLENIAYVTTFYDHNLLGHI
                     HVNWLAPVKVRKTLLSGSKKMVIYDDMEPSEKVKVYDRGIDFVRDKDQIYNILIEYRT
                     GDMWAPKVELTEALRKVTAE"
     gene            complement(905..>1457)
                     /locus_tag="PK112_08525"
     CDS             complement(905..>1457)
                     /locus_tag="PK112_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12127.1"
                     /translation="LNSGAGRRNTFSLQIGEATIPLVGGQPPLAEFFYPKPTYRYYAQ
                     LIPMGLEEFRQYAVIPVPDSMVRRLIGEQGYLEVSVAINGSLAEENNFVTLWGRYDGD
                     ESTGLIPGWRRTSIERFVHQNDPRLPQPVKFVSDSAVCYYIPRADGDPSAGGDLAPAW
                     GRQTGRYNLFLMHFKADGSVDVY"
BASE COUNT          253 a          467 c          455 g          282 t
ORIGIN      
        1 attcggcggt cactttccgc agcgcctccg tgagctcgac tttcggcgcc cacatgtcgc
       61 cggtgcggta ctcgatgagg atgttgtaga tctggtcttt gtcgcggacg aagtcgatcc
      121 cccggtcgta cactttcact ttctccgagg gctccatgtc gtcgtagatc accattttct
      181 tcgaccccga caggagcgtc ttgcgcactt tcaccggcgc cagccagttg acgtggatgt
      241 gcccgagcag gttgtggtcg tagaacgtgg tcacgtaggc gatgttctcg agatcgcggt
      301 cgaagtgggc cacgccggtc gccgaaatcg ccctgggatc ccgtcccagc aggtaatcca
      361 tgatcgacac gtcgtgagcc gcgagatccc agatgacgtt gacgtcgtgc tggaagagcc
      421 caagattaac ccgcaccgag tcgaaatagt acagctcgcc cagctccccg ccgtcaacca
      481 gccgcttgat cgtctgcacc gccccggtgt agaggaaggt gtggtcgacg aagaggcgaa
      541 ggtttttgcg ctcggcggtc tcgatgagcc gttcggcctg ctcgactgtg gccgtgaagg
      601 gcttctcgag aaacacgtgt ttgcccgcct ccagcgcgtc catggccagg gcaaagtgcg
      661 acgacaccgg cgtggcgatc acgacggcgt cgatttcggg cgcccggatc acctccccgg
      721 catcggccga acaccgggtg atcgtggggt actgccgggc gatcagattg agccgttcgc
      781 ggcgcaggtc ggcgatcgcc tgcaccttca tctcggggtg ggccgcgatg ttccggatga
      841 gattcggtcc ccagtagccg cagccgatga cgcctacctg catggccatt ggtcccttcg
      901 cttgtcagta tacatccacc gagccgtccg ccttgaaatg catgagaaac agattgtacc
      961 gtccggtctg ccggccccag gccggcgcga ggtcgccgcc ggcgctcggg tcgccgtcgg
     1021 cccgcggaat ataatagcaa accgccgaat ccgacacgaa tttcacgggc tgcggcagcc
     1081 gcggatcgtt ctgatgcacg aaccgctcga tcgaggtccg gcgccacccc gggatcagac
     1141 ccgtcgattc gtcgccgtcg taccgtcccc agagggtgac gaagttgttc tcttccgcca
     1201 gcgagccgtt gatcgccacg ctcacctcca ggtacccctg ctccccgatc agccgccgca
     1261 ccatggaatc cggcaccggg atcaccgcgt attggcggaa ttcttcgagg cccatcggga
     1321 tcagctgggc gtagtagcga taggtcggtt tggggtaaaa gaactcggcc aaggggggct
     1381 gcccgccgac cagcggaatg gtggcctctc cgatctgcag ggagaacgta ttccgccgcc
     1441 cggctccgga gttgaga
//
LOCUS       DAOOKS010000565         1450 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_116487_length_1450_cov_2.600000, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000565 DAOOKS010000000
VERSION     DAOOKS010000565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116487_length_1450_cov_2.600000"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            536..1363
                     /locus_tag="PK112_08530"
     CDS             536..1363
                     /locus_tag="PK112_08530"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HPC12128.1"
                     /translation="MKPVYHFVRLLGTRAGWPDDMTPREEQVMANHFAYLKDLTRKGI
                     VVAAGPVFNPTFGLIILRTGSEAEARALMDSEPSVAAGVHTYELQPMTLSLLADHRSP
                     LRYVSRPSARSRRKEIVVPVSADSAWTLWTTNAGLEAFLVEKANVDLRLGGSFELYFS
                     MASPVGQRGSEDCAILSFLPGRMLSFEWNAPPSFPSRRDGERTWVVVEFEPLAPDRPA
                     ADSGVAPAAPAAAQTRITLTHLGWGEGEDWDSLYAYFDRAWGLVLDALREYLAGTAE"
BASE COUNT          230 a          511 c          446 g          263 t
ORIGIN      
        1 cctcctgggc cagggcggcc ccgcccagga gcagacatcc cagaagcacg catccgaaaa
       61 atcgtgtccg cataccatac ctcccgcaaa cgcttagcgg tgacaaggac tcccaagatc
      121 taatgaatgt tctcattgtg atccttctcg gtggacgccc ggccgggaaa ccgtcgatcg
      181 atatgccgag gcgcgccccc tcgatcgcgg gcaggagaag cggcggtcgc cgatccggac
      241 ggcggaaaat gatccgtgat tcggctgatt tctaattcaa catagcgtgg atctggtagt
      301 ctgtcaagtg cttttcccgc gtggcccgcg cccctctcgc gcttgcgccg accgtcgcca
      361 acgcctatgt tgtcgctgcc gtttgcggtc cgcaacaacc gacaggagga ttctttagtc
      421 tatgctgtgc cgctgtgatc ctcaccccag gcgccgcgcc gtcgcggccg gcgtcgtcag
      481 cctgcttctc attctgtccg ccggcgcccc tgccgctcaa ggagacaatc ccgatatgaa
      541 acccgtctat cacttcgtcc gcctgttggg cacccgcgcc gggtggcccg acgacatgac
      601 cccgcgcgag gagcaggtca tggccaacca tttcgcctac ctcaaggacc tgacgcggaa
      661 aggaatcgtg gtggcggccg ggccggtctt caaccccact ttcggcctga tcatcctgcg
      721 caccgggtcc gaggccgagg cgcgcgccct catggacagc gagccttcgg tcgcggccgg
      781 cgtgcacacc tacgaactgc agccgatgac gctctccctc ctcgccgacc accgctcgcc
      841 gctgcgctat gtctcccgtc cgagcgcgcg ctcgcggcgc aaagagattg tcgtccccgt
      901 ctccgccgac tcggcctgga ccctctggac gaccaatgcc ggcctggagg ccttcctggt
      961 cgagaaagcc aacgtcgacc tgcgcctcgg cggctcgttc gagctgtatt tctcgatggc
     1021 ctccccggtc ggccagcgcg ggagcgagga ctgcgcgatc ctgagcttcc ttcccggacg
     1081 gatgctctcg ttcgagtgga acgctccccc ctcgttcccg tcccgccgcg acggcgagcg
     1141 cacctgggtg gtcgtcgaat ttgaaccgct ggctcccgac cggccggccg ccgactcggg
     1201 tgtggccccg gccgcgccgg ctgcggcgca aacccgcatc accctcaccc acctcggttg
     1261 gggggagggg gaagactggg acagtctcta tgcgtacttt gaccgcgcct gggggctggt
     1321 tctcgatgct ctgcgggagt acctggcggg tacggccgag tgagggtggc ggcgccggcg
     1381 cgcggcgccc cggcgcgtgt ataacgaata acgagacttg tccccgacag aggagcgcaa
     1441 tacattaatt
//
LOCUS       DAOOKS010000566         1445 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_117055_length_1445_cov_2.848921, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000566 DAOOKS010000000
VERSION     DAOOKS010000566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117055_length_1445_cov_2.848921"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="PK112_08535"
     CDS             complement(<1..232)
                     /locus_tag="PK112_08535"
                     /inference="COORDINATES: protein motif:HMM:NF018648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HPC12129.1"
                     /translation="MRETTDRPLSRLTRPLARFLHTESSSGIVLLVCTAAAVAAANSP
                     LADSWMRFWNTVLTVGAGRFSLSYPLWYWVNDG"
     gene            complement(419..1018)
                     /locus_tag="PK112_08540"
     CDS             complement(419..1018)
                     /locus_tag="PK112_08540"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HPC12130.1"
                     /translation="MKKALITVAALLLMSAGVSAQVENPISLYVGGAISIPNSPEEFS
                     EAYNTGFHGSVGLGLAVMPSLQVVGKVEYHRFGLDVTQDMEDMGVTGGHNNMWMYGAD
                     ARYSFGLPAAPIKPFLLGGVGFARISASDLEGTGDLVAGLNELNPDAETNLYLNLGGG
                     VELKTGPMFSFFGQVRYVTVDTDGESSSFIPVTLGLKFF"
     gene            complement(1259..>1445)
                     /locus_tag="PK112_08545"
     CDS             complement(1259..>1445)
                     /locus_tag="PK112_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12131.1"
                     /translation="EGYDFELLDSLDLARHHPRIIRFENLHMTGAFYAAAIRKLPAAG
                     YRFVANRMDTTAYATLP"
BASE COUNT          286 a          439 c          481 g          239 t
ORIGIN      
        1 gcccgtcgtt aacccagtac cagagcggat aggagagaga gaagcggccg gcgccgaccg
       61 tgaggacggt gttccagaag cgcatccacg aatcggccag gggcgagttg gcggcggcca
      121 cggctgccgc cgtgcacacc aggagcacga tcccgcttga ggattcggtg tggaggaagc
      181 gcgccagcgg gcgggtgagc cgcgacagcg gccggtcggt cgtctctctc atggatcctc
      241 tcctctgagg gttcgtaccg ataataccgc acgatcccgg cgcgggcaag ccggttcggc
      301 gcccggccgg gagaatcgtc accggcccgg cgcgaacggg aagggagttg acgagcgggc
      361 acaaaaaaac cggggcggct ggccgccccg gcgcataatc tctgccgaca acccgcacct
      421 agaagaactt caggccaagg gtcaccggga tgaacgacga cgactcgcca tcggtgtcta
      481 ccgtgacgta gcggacctgc ccgaagaagc tgaacatcgg accggttttc agctcgacgc
      541 cgccgccgag gttcaggtag aggtttgtct cggcgtcggg gttgagctcg ttgaggccgg
      601 cgaccaggtc acccgtgcct tcaagatcgg aggctgagat gcgggcaaaa ccgacgccgc
      661 ccaggaggaa cggcttgatc ggcgccgccg gcagaccgaa cgagtaccgc gcatcggcgc
      721 cgtacatcca catattattg tggccgccgg tcacccccat gtcctccatg tcctgtgtca
      781 catcgagccc gaaccggtgg tactcaacct tgccgaccac ctgcaagctc ggcatcacgg
      841 ccagacccag accgaccgag ccgtggaaac cggtgttgta ggcctccgag aactcctccg
      901 gcgaattcgg gatggagatg gcgccgccga catacaggct gatcgggttc tcgacctgag
      961 ccgagacgcc ggccgacata agcaggagag ccgcaactgt aatcaacgct ttcttcatgt
     1021 cctactcctt acgctttgtt gcctttagtg tggtcattgc atagatcggc cgattcgttc
     1081 cggaacttga ctcccggccg agaaatttct ccccccgcaa gtcactgccg cagaacatat
     1141 tatcggtaag acgaggggcc gccactgacc gggaaagcgg gggaaagggg atgcgcgcac
     1201 cttggctacg gggaatcggg ttgaggtctt gcggacggat cggcgccgcg ggcgcgcctc
     1261 acggcagggt ggcgtaggcg gtggtgtcca tccggttggc gacgaaccga tagcccgcgg
     1321 ccggaagttt ccggattgcg gcggcgtaga atgcaccggt catgtggaga ttctcgaagc
     1381 gaatgatccg cggatgatgg cgcgcaagat cgaggctgtc gaggagttcg aagtcgtaac
     1441 cttcc
//
LOCUS       DAOOKS010000567         1441 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_117658_length_1441_cov_1.228716, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000567 DAOOKS010000000
VERSION     DAOOKS010000567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117658_length_1441_cov_1.228716"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="PK112_08550"
     CDS             <1..181
                     /locus_tag="PK112_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12132.1"
                     /translation="GAASGTGGRVALHVVKLASALETSMSFAEFVSIDHPKPQKFRRY
                     AGQNPADALGKQSRT"
     gene            483..1283
                     /locus_tag="PK112_08555"
     CDS             483..1283
                     /locus_tag="PK112_08555"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HPC12133.1"
                     /translation="MVKKAPAYDVPGLRAGIRISRLLCESPKPLGLAELCQASGLNKH
                     MVYRCIQTLLSEGMLIETGEGPKYEPSLLSFHYNSMPVARMTVTRAAREPLHALWEEL
                     NECVYLGVPHDDRVLYLIHHEGGREIRSGGGRMGGHYYLHADGAGKVLAAYGDDALRG
                     RLLKEGLKKLTPNTHIDRKRFLDEMARVRRQGFAFDNEEYIKGVICFGAPVFDYTGSV
                     AGAICSSVLTIHYTFKELEAVIGPKVLACARRISATMGWAGEKGGERA"
BASE COUNT          285 a          408 c          474 g          274 t
ORIGIN      
        1 cggtgccgca tcggggacag gtgggcgggt tgcgcttcat gtcgtaaaac ttgcatccgc
       61 actcgaaaca agcatgtcgt tcgccgaatt tgtctctatc gaccatccca aacctcaaaa
      121 gtttcgaaga tacgccggac aaaatccggc ggacgcgcta ggtaaacaat cacggacgta
      181 atgtcaagca tatcgaacac gagatgatgt tcgtcgtcaa cattttcgtg atgcccgtct
      241 cgccggaacc ccttgacctg cgcccgggcg tatttatatt gcggggcggt cgaaccgcct
      301 gtttgaaagg tgaccgggga ttgattccca cggatgaagg tcaaggtaca aaccacggag
      361 atatgaaatg gcagcgaagg atcttgcatt ttcggcggtt ctgatggccg tggccgtttg
      421 ggtgccgtgt ggttgagggc ggggggttga cacctgtcgg ttcattagtg taccatcggt
      481 tcatggttaa gaaagcgccc gcttatgacg tgccggggtt gcgggccggc atccggattt
      541 cgaggctgtt gtgcgagtcg ccgaaaccgc tcggcctggc cgaactgtgc caggcgtcgg
      601 ggctcaacaa gcacatggtg taccgctgca tccagaccct gctctcggaa ggcatgctca
      661 tcgagaccgg cgagggcccc aagtacgagc cgtcgctgct ctcgttccac tacaactcca
      721 tgccggtggc gcgcatgacc gtcacgcgcg ccgcgcggga gccgctccac gcgctctggg
      781 aagagttgaa cgagtgcgtc tacctcggcg tgccgcacga tgaccgcgtc ctctatctga
      841 tccaccacga gggcgggcgc gagatccgct ccggcggcgg gcgcatgggc ggtcactatt
      901 acctgcacgc ggacggcgcc ggcaaggtgc tcgcggccta tggggacgac gcgctgcgcg
      961 ggcggctgct gaaggaggga ctgaaaaaac tcacgcccaa cacgcatatt gaccggaaac
     1021 ggtttctgga tgagatggcc cgtgttcgca ggcaggggtt cgccttcgac aacgaagagt
     1081 acatcaaagg cgtcatctgc ttcggcgcgc cggtcttcga ctacaccggg agcgtggccg
     1141 gggcgatctg cagctccgtg ctgaccatcc actacacctt caaggagttg gaggcggtga
     1201 tcggcccgaa agtcttggcg tgcgcgcggc ggatctccgc aacgatgggc tgggcgggag
     1261 aaaagggagg ggagagggca tgatcaatct ggcgaaaagg gaccggcggt cggtcggaaa
     1321 actgttcgac tactctgtgc ttccgaaaca gacgtcggaa gcggagatcc ggaaagggtg
     1381 ccgcgaggcg gtcgcctaca actgcgcggc cttttactcg gcctcgccgt actggactcc
     1441 g
//
LOCUS       DAOOKS010000568         1440 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_117692_length_1440_cov_3.761733, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000568 DAOOKS010000000
VERSION     DAOOKS010000568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117692_length_1440_cov_3.761733"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="PK112_08560"
     CDS             <1..334
                     /locus_tag="PK112_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12134.1"
                     /translation="RYRVSRVVAVGAEAAVRSGYAYTLPPKSPAAPDGDRLTVDYYRA
                     ALARENGARFPTHTTLNLNAVFDFGAAEAYCAIANITNRANPIIRSADGFIYDAGILP
                     SAGVTVRF"
     gene            complement(364..639)
                     /locus_tag="PK112_08565"
     CDS             complement(364..639)
                     /locus_tag="PK112_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12135.1"
                     /translation="MSALTYVLTLGALIGVVWHVIATMAIYNYLRRRGDKVSFVWLRL
                     MSPIYASRYRAATRQETGRVGHLFYHWVIPINLAFLLVVLAVASRYV"
     gene            complement(785..1387)
                     /locus_tag="PK112_08570"
     CDS             complement(785..1387)
                     /locus_tag="PK112_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12136.1"
                     /translation="MEENRYKLAGWLAIVQAVLFPVAIVIGLIEAGVAAEFFNIRRPF
                     VGPSDFLMIPFTAIAVYTLLKFRSLLHERYAYRDLDLLIIVSVWWTIAFQVCGLAFGI
                     LTSAFWPVDRVVLLVGFVAFFTGAMVSIGVVDILIAVKLLGAKERFGEYIRGFAYVTM
                     AAGICEVTVLMSVLALLLVPVSAIVLALIFFRDQQEVEFV"
BASE COUNT          321 a          466 c          448 g          205 t
ORIGIN      
        1 gcgctaccgg gtgagccgcg tggtggcggt cggggcggaa gccgcggtcc ggtcgggata
       61 cgcctacacc ctgccgccaa aaagcccggc ggccccggac ggggaccgct tgaccgtcga
      121 ctactaccgg gccgccctgg cgcgggagaa cggcgcgcgc ttccccacgc acacgactct
      181 gaatctgaat gcggtgttcg acttcggcgc ggccgaggcc tactgcgcca ttgccaacat
      241 caccaatcgg gccaacccga tcatccggtc cgccgacggt ttcatctacg acgccgggat
      301 cctgcccagc gccggcgtga ccgtgcggtt ctgaagcctg aagcccgctt ttcctacaaa
      361 gagctacacg taccgggagg cgacggcgag cacgaccagc agaaacgcca gattgatcgg
      421 gatcacccag tggtagaaca ggtggccgac cctgccggtc tcctgccggg tcgccgcgcg
      481 gtaccgcgag gcgtagatgg ggctcatgag gcgcagccac acaaaactca ccttgtcgcc
      541 gcggcgccgg aggtagttat agatggccat agtcgcgatc acatgccaga ccacaccgat
      601 caaggccccc agggtgagga cataggtcag ggcgctcata cgtgctcctt tctgccgggg
      661 cgggccatca acccgccgcg cccaaagaac ggcgatacgc cggccgcgag gaccggggcc
      721 atgaatacgg cgcccgcagg accgaacccg gcctctcgaa cgggagtgag cgcgttccgg
      781 tttttcacac aaactccact tcctgctggt cgcggaagaa aatgagggcg aggacgatgg
      841 ccgagacagg cacgaggaga agggccaaca cggacatcag cactgtgacc tcgcagatcc
      901 cggcagccat cgtgacatag gcgaagccgc ggatgtattc gccgaagcgt tccttggccc
      961 cgagcagttt gaccgcgatc agaatatcca cgacgccgat cgagaccatg gccccggtga
     1021 aaaaggccac aaacccgacc aggagcacca cccgatcgac cggccagaag gcggaggtca
     1081 ggatgccgaa cgccagcccg cagacctgaa acgcgatcgt ccaccagacc gagacgatga
     1141 tcaggaggtc gagatcgcgg taggcgtaac gctcgtgcag gagcgagcgg aatttcagca
     1201 gcgtgtagac cgcgatcgcc gtgaacggaa tcatgagaaa atcggagggg ccgacaaacg
     1261 gccggcgaat gttaaaaaac tcggcggcta cgcccgcttc gataagaccg atcacgatgg
     1321 cgacgggaaa gagcacggcc tgcacgatcg caagccaacc ggccagctta tacctgttct
     1381 cttccatact tccaagaccc cgccttcagg atgcgtgagg ttctctgtct cctaaacaaa
//
LOCUS       DAOOKS010000569         1439 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_117834_length_1439_cov_3.250723, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000569 DAOOKS010000000
VERSION     DAOOKS010000569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117834_length_1439_cov_3.250723"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(245..481)
                     /locus_tag="PK112_08575"
     CDS             complement(245..481)
                     /locus_tag="PK112_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12137.1"
                     /translation="MAVEDPRQPVVHRRPRRRVDRLELDREFGPVLCLLPRARQQHGV
                     RHLEDHAVDHPADRDLEIGRPADPFPRVEAGLKD"
     gene            complement(725..1147)
                     /gene="mce"
                     /locus_tag="PK112_08580"
     CDS             complement(725..1147)
                     /gene="mce"
                     /locus_tag="PK112_08580"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HPC12138.1"
                     /translation="MISHVGIAVADLDAAVARYRLLTGGAAPVIVDVPDQKVRVAIFS
                     GGEGAPGHGGGRIELVAPVSDDSPVAKFIAKRGEGLHHICLYVADIERKLAELKAAGA
                     RLIDETPRCGAEGNRIAFVHPAGSHGVLLELEEHPTAL"
     gene            complement(1159..>1439)
                     /locus_tag="PK112_08585"
     CDS             complement(1159..>1439)
                     /locus_tag="PK112_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12139.1"
                     /translation="DYVTQPYIANASADSLVRIEVTSVERLGDSAVARVLVHFDNAGR
                     KSVIDDKVTLYWHQGKWIKPTISRLKDQAAFDSVRNAAIEAAKKESGN"
BASE COUNT          202 a          447 c          543 g          247 t
ORIGIN      
        1 cgaagagccg ggaggaattc atcggtgagc gggtgcggat cgtccgggag gtcctccgca
       61 agaatttcgc aggggacgca tgatcctcgc tctcgtcgcg gtgctggcgc tggcggtggc
      121 gggcaaccgc ttcgtcagcc tccccgactc cgtcacgctg ccggcccccg ggggctggcg
      181 ggtgctgggc gagccggcgt cgtatccggc ggtgctgcac aacgaagcaa gcggggcgcg
      241 gctgctagtc tttgagaccc gcctcgaccc gggggaacgg atcggcgggg cggccgattt
      301 ccaggtctcg gtcagccgga tgatcgacag cgtggtcctc caaatgccga acgccgtgct
      361 gctgacgagc acggggcagc aggcagagaa ccgggccgaa ttcgcggtcg agttccagac
      421 gatcgacacg gcgacggggg cggcggtgta ccaccggctg gcggggatcc tctaccgcca
      481 tcccgacgga tcgcagctcc tgttcagcct gtgggcgcag gccccgcccg gggcggacgc
      541 ggcgctgcgg gcggagctgg agcggatgca gtcgggtttc cgctacaacg ggccgcagga
      601 ggcgcaggta tttgcgccgg cgcgctccga tatgcagtgg tttctcgtct tctcatcgga
      661 gcggcggcgg tggtgctgtt cttgcggcag cggcggcgcc tcgcgcgggc ggcgcccgag
      721 ccgcctagag cgccgtaggg tgttcctcca gttcgagcag gacgccgtgg ctgccggccg
      781 ggtggacgaa ggcgatgcgg tttccctcgg cgccgcagcg gggagtctcg tcgatgagtc
      841 tcgccccggc cgctttcagt tcggccagtt tgcgctcgat gtccgcgaca tagaggcaga
      901 tgtggtggag gccctcgccg cgtttggcga tgaatttggc caccgggctg tcgtcggaga
      961 cgggggcgac cagttcgatg cggccgccgc cgtggccggg agcgccttcc cccccggaga
     1021 agatcgcgac tctcactttc tggtcgggga cgtccacaat gacgggggcc gcgccgccgg
     1081 tgaggaggcg gtaccgggcg acggcggcgt cgaggtcggc gaccgcgatg ccgacgtggg
     1141 agatcatgtc tgaagccatc agtttccgga ctccttcttg gcggcctcaa tcgcggcgtt
     1201 gcgaacgctg tcgaaagcgg cctggtcttt cagtcgggag atcgtcggtt tgatccactt
     1261 cccctggtgc cagtagagcg tgaccttgtc atcgatcacc gatttccgcc cggcgttgtc
     1321 gaagtgaacg agcacgcggg cgacggccga gtcgccgagg cgctccaccg aggtcacctc
     1381 gatgcggacg agggagtcgg ccgaggcgtt cgcgatatag ggctgcgtga cgtagtcgt
//
LOCUS       DAOOKS010000570         1430 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_119062_length_1430_cov_2.916364, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000570 DAOOKS010000000
VERSION     DAOOKS010000570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1430
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119062_length_1430_cov_2.916364"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..246)
                     /locus_tag="PK112_08590"
     CDS             complement(25..246)
                     /locus_tag="PK112_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12140.1"
                     /translation="MRYRALAGLLILGAGVLVLACGKGGSEADGPPDRVPPETKVAEM
                     KDSTRFDPAEPEDTAAHDPEEGEPATPPE"
     gene            complement(272..484)
                     /locus_tag="PK112_08595"
     CDS             complement(272..484)
                     /locus_tag="PK112_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12141.1"
                     /translation="MKKLLVGGLVLLWALTCAFGCGGGEKAEPQTETQMEPAPEQTAP
                     PAAESVAVDTMATPDSAVTDTGALGE"
     gene            719..>1430
                     /locus_tag="PK112_08600"
     CDS             719..>1430
                     /locus_tag="PK112_08600"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HPC12142.1"
                     /translation="MDTSIAPFILAGPIWNIISNSSAFGIFILGVLVFMSLTSWAIIF
                     AKYREFRAVEAENNKFLQTFRRARSLSEAIGQAKSHSSAPVSHIFLAGYNELTEIVGA
                     KNEGGGLQEVKHSLADEDLASVEMVMDKTHNEQIGKLERRVIFLASTANSAPFMGLLG
                     TVVGIMDAFWSIGERGSASLAVVAPGIAEALLATIVGLGAAIPAVIAYNWANSKLKYA
                     NDFSQNFILEFLARVRREV"
BASE COUNT          236 a          495 c          373 g          326 t
ORIGIN      
        1 ctcaggacgc ttcgcggctt ttcgttattc aggcggcgtc gccggctctc cctcttcagg
       61 gtcgtgagcc gccgtatctt ccggctcggc cgggtcgaaa cgcgtgctgt ctttcatctc
      121 cgccactttg gtctcgggcg gcacgcgatc agggggccca tcggcctccg atcccccctt
      181 gccgcacgcg agcaccagca cgcccgcccc caggatcaac agccccgcca gcgctcggta
      241 ccgcatgtcc ccgatccctc gacctctccc gctactcgcc cagggcgccc gtgtccgtca
      301 ccgccgagtc cggagtcgcc atcgtatcta ccgccacgct ttccgctgcc ggtggcgccg
      361 tttgctccgg agccggctcc atctgggtct cggtctgcgg ctctgctttc tcgccgccac
      421 cgcacccgaa agcgcaggtc agcgcccaca ataggactag tccgccgaca agtagcttct
      481 tcatctgccc gctctcctct ccttgagtcc ggttaacgta tgctgtctct ccttttccac
      541 ctgcaaagct gcttgagatc gaagattttc ccccgcccgt gctcagtcaa gtttttaatt
      601 ccgcctgttt tagttgaccc tgccggatcc tcccgatatg ttgcggctcc gatttcggaa
      661 acgcggatgg attccgtccg cctcgaaaac tttaggtgaa acgggtgttt aaaagaatat
      721 ggatacttcg attgctccct tcatcctcgc cggcccgatc tggaatatca tatcgaattc
      781 gtccgcattt ggcatcttta ttctgggcgt ccttgtgttc atgtcgctga cgtcctgggc
      841 catcatcttc gccaagtacc gcgagttcag agcggtcgag gccgagaaca acaagttcct
      901 ccagactttc cgtcgcgccc gctctctgtc ggaagctatc ggccaggcga aatcgcactc
      961 ctccgccccc gtctcccaca tcttcctcgc cggctacaat gaacttactg agatcgtcgg
     1021 ggccaaaaac gaaggcggcg gtttgcagga agtcaaacac agtctggccg atgaggatct
     1081 ggcgtcggtt gagatggtca tggacaagac ccacaacgag cagatcggca agttggagcg
     1141 gcgcgtcatc ttcctcgcct cgactgccaa ctccgctccg ttcatgggct tactcgggac
     1201 tgtcgtcggt atcatggatg ccttctggtc gatcggcgaa cgcggctccg cctccctcgc
     1261 ggtggtcgcc cccggtatcg ccgaagctct cctggccacg attgtcggcc tcggcgccgc
     1321 catcccggct gtcatcgcct acaactgggc gaacagtaag ctgaaatacg ccaacgactt
     1381 ctcccaaaat ttcatcctcg agttcctcgc ccgggtgaga cgggaggtcc
//
LOCUS       DAOOKS010000571         1430 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_119086_length_1430_cov_2.615273, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000571 DAOOKS010000000
VERSION     DAOOKS010000571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1430
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119086_length_1430_cov_2.615273"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="PK112_08605"
     CDS             <1..474
                     /locus_tag="PK112_08605"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HPC12143.1"
                     /translation="CYAAEAGDSAFAAVMSQPPTVSGEGWTWLTDGDLVTLEPGAAPL
                     VERIEQYFDGTGADGEPPALPRAMALEQNYPNPFNASTVIAFELPARAAVRLAVCSAL
                     GRHVATLADGVRGAGRHEVRWDGRDDSGKPVASGVYLCRLEGAGRSAVRKMIVLK"
     gene            607..1227
                     /gene="pdxT"
                     /locus_tag="PK112_08610"
     CDS             607..1227
                     /gene="pdxT"
                     /locus_tag="PK112_08610"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HPC12144.1"
                     /translation="MSEYGKLTVGVLALQGDFERHLYQLRRVGAKAREVRRPADLEGL
                     DGLIMPGGESTTMNILMDRFALREPLRAFAQTRPVWGTCAGMIMAAREIEDNQAGVRP
                     LGLIDITVVRNGYGRQVFSFEAHLAARLGPAATALAATFIRAPRVTRVGPGVTALAEY
                     RGAPVLVAAGRVLASSFHTELGEDTTLVEYFLRKYLLRTAAANIYS"
     gene            1228..>1430
                     /locus_tag="PK112_08615"
     CDS             1228..>1430
                     /locus_tag="PK112_08615"
                     /inference="COORDINATES: protein motif:HMM:NF028191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12145.1"
                     /translation="MQKVFFTQSVGDRSAHAPSPQAPPRRERAMVPGVRLPRWMKTRA
                     GYSPNYDRIKNLLVAHGLHTVCQ"
BASE COUNT          222 a          431 c          550 g          227 t
ORIGIN      
        1 tgctacgccg ccgaggccgg ggactcggcc tttgcggcgg tgatgtcgca gccgccgacg
       61 gtctcggggg agggttggac gtggctgacc gacggcgacc tggtgacgct cgaaccgggg
      121 gccgcgcccc tggtggagcg gatcgagcag tacttcgacg gcacgggggc ggacggcgag
      181 ccgcctgccc tgccgcgggc gatggcgctc gagcagaact acccgaaccc gtttaacgct
      241 tcgaccgtga tcgccttcga gttgcccgcg cgcgcggcgg tgcggctggc tgtttgcagc
      301 gcactcggcc gccatgtcgc aactttggcc gacggcgtcc ggggggcggg acggcacgag
      361 gtgcgctggg atggacggga cgacagcggc aagccggtcg ccagcggcgt ttacctctgc
      421 cgcctcgaag gggcaggacg gtcggccgtg cgaaagatga tcgttctcaa ataggggggc
      481 cggggagacc ggtcggggcg catgacgggc ttgaccgccg gcggggaggg gactttattt
      541 cgggcacttt tgacgcccta tagttgttgc tgaagcctga gatgagacgg ggaacgcgga
      601 accgccatga gtgaatacgg caaactgaca gtcggcgtgc tggcgctcca gggggatttc
      661 gagcgccatt tgtaccagct ccggcgggtc ggggcgaaag cgcgggaagt gcggagaccg
      721 gccgacctcg aggggctgga cgggctgatc atgccgggcg gcgagtcaac cacgatgaac
      781 attctcatgg accggtttgc gttgcgtgag ccgctgcgcg cgttcgcaca gacgaggccg
      841 gtgtggggca cctgcgcggg aatgatcatg gcggcgcggg aaatcgagga caaccaggcc
      901 ggggtgcggc cgctcgggct catcgatatc acggtcgtgc gcaacggcta cgggcggcag
      961 gtcttctcgt tcgaggcgca cctggcggcg cgcctgggcc cggccgcgac ggcgctggcg
     1021 gccaccttta ttcgggcgcc ccgcgtgacc cgggtcgggc cgggggtgac cgccctggcc
     1081 gagtaccggg gggcgccggt cctggtggcg gccgggcggg tgctggcgag ctcgttccac
     1141 accgagctgg gggaagacac gacgttggtg gaatatttcc tgcgaaaata cttgttgcga
     1201 acggccgccg ctaatatata ttcgtagatg caaaaagtct tttttaccca gtcggtcggc
     1261 gaccgctcag cccacgcgcc gtcgccccag gcccctcccc ggcgggagcg ggcgatggtg
     1321 ccgggagtgc ggctgccgcg gtggatgaag acgcgggccg gctattcgcc aaactatgac
     1381 cggatcaaga acctgctggt cgcccacggg ctccacacgg tctgccagga
//
LOCUS       DAOOKS010000572         1425 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_119752_length_1425_cov_2.729197, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000572 DAOOKS010000000
VERSION     DAOOKS010000572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119752_length_1425_cov_2.729197"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="PK112_08620"
     CDS             <1..527
                     /locus_tag="PK112_08620"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598180.1"
                     /note="catalyzes the formation of glycerone phosphate and
                     D-glyceraldehyde 3-phosphate from D-fructose
                     1,6-bisphosphate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HPC12146.1"
                     /translation="RQLQEMTAAFKYAHELGMFTVLWCYLRNPAFKKDGVDYHTAADL
                     TGQANHLGVTIEADIVKQKSPETNAGYKAIGFGKTSEKVYTELTTDNPIDLTRYQVVN
                     CYMGRVGLINSGGESKGKGDLAAAVRTAVINKRAGGTGMISGRKAFQRPIKEGIELLN
                     AIQDVYLEKDVTVA"
     gene            648..>1425
                     /locus_tag="PK112_08625"
     CDS             648..>1425
                     /locus_tag="PK112_08625"
                     /inference="COORDINATES: protein motif:HMM:NF027806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12147.1"
                     /translation="MRLCYLVLLTAFLAIPAAALAAEPATGGSADTLGPTTVETAAPA
                     LDTAAAAGAATPEYPMDPERKAKLISYSRFVNIWRFADLIITCGILALILFTGLSARM
                     RTLAQRIFRRRGLAVAGFVVLFMAVDFLLNLPFSIYRNYMVESDYGFVNQSFGGWFGE
                     AVLSLVVACVIAIIAVSFLYWVINRFRRWWLVFMIGAIPFTIFFIVIAPVLISPLFND
                     FVPIQNQGLGTKLVNLAESVGIHDPDVFQINASKQSSKINA"
BASE COUNT          251 a          434 c          463 g          277 t
ORIGIN      
        1 tgcgccagct ccaggagatg acggcggcgt tcaagtacgc ccacgagctc gggatgttca
       61 ccgtgctctg gtgctacctg cgcaatcccg cgttcaagaa agacggcgtc gactaccaca
      121 cggcggctga tctcaccggc caggcgaacc acctcggggt gacgatcgag gcggacatcg
      181 tcaaacagaa gtcgccggag accaacgccg gctacaaggc gatcgggttc gggaagacca
      241 gtgagaaagt ctacaccgaa ctgacgaccg acaacccgat cgatctgacc cggtaccagg
      301 tggtcaactg ctacatgggg cgggtcggcc tgatcaactc cggcggcgag tcgaagggga
      361 agggggatct cgcggccgca gtgcgcacgg cggtgatcaa caagcgcgcc ggcgggaccg
      421 gcatgatctc cggccggaaa gcgttccagc ggcccataaa ggaagggatc gagctgctca
      481 atgcgatcca ggacgtctac ctggagaagg acgtgacggt ggcgtaggcc gcccggcggc
      541 gaataccggg aggcgggtcg tggatgcccg cctctttttt tttgcacccc ggccgccgtt
      601 gtcccgtata ctcctgcatt acgattctca cccggaggta aacgacaatg cgtctgtgct
      661 atctggttct cctcactgcg ttcttggcga ttccggcggc ggctctggcg gccgagccgg
      721 cgaccggcgg gtcggccgac acgctcggcc cgacgacggt cgagacagcg gcgccggcgc
      781 tcgacacggc cgcggcggcg ggcgcggcga cgccggagta tcccatggat cccgagcgga
      841 aagcgaagct gatttcgtac tcgcgctttg tcaacatctg gcgctttgcc gacctcatca
      901 tcacctgcgg gattctggcg ctgatcctgt tcaccggcct gtcggcccgg atgcgcacgc
      961 tggcgcagag gatttttcgg cggcgggggc tcgcggtcgc cggattcgtc gtcctgttca
     1021 tggcggtcga tttcctgctc aatctcccct tcagcatcta ccgcaattac atggtcgagt
     1081 cggattatgg atttgtgaac cagtcgttcg gcgggtggtt cggggaggcg gtcctgagcc
     1141 tggtggtcgc gtgcgtcatc gcaattatcg ccgtgtcgtt tctctactgg gtgatcaacc
     1201 ggttccggcg gtggtggctg gtcttcatga tcggggcaat cccgttcacg atctttttca
     1261 tcgtgatcgc gccggtcctg atcagcccgc tgttcaatga cttcgtgccg atccagaacc
     1321 aggggctggg gaccaagctg gtgaacctcg cggagagcgt ggggattcac gaccccgatg
     1381 tcttccagat caatgcctcc aagcagtcgt cgaaaatcaa cgcct
//
LOCUS       DAOOKS010000573         1419 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_120561_length_1419_cov_2.762463, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000573 DAOOKS010000000
VERSION     DAOOKS010000573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120561_length_1419_cov_2.762463"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1027)
                     /locus_tag="PK112_08630"
     CDS             complement(<1..1027)
                     /locus_tag="PK112_08630"
                     /inference="COORDINATES: protein motif:HMM:NF028512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPC12148.1"
                     /translation="MFRRPVKEINAEVVAEEKYLDSLQRITREACLSAGLSRKEVSPI
                     LLAIEEGATNIIRHAYLYEKGTIRLRIVIYKKMIVFSLIDTGRSYQPESDGKLDLQRL
                     VESGRKGGLGFYMIKKIMDSVEYISSAGYNELRMIKQAQPVASESIPFLRHMATLRAK
                     FSVWTFLIVLVIVGGAYYYFDSQTTRQMKSHLDETVASLVNTIGDQAAGYVINRRSDV
                     EFDELIVSAVRSNAGILRQVVLTDTAGTILAHSDDIHNIRKPYAPPPEVHAARVGVPF
                     RFSENGRALSYLMLPIRQGDRSFGTVHAVYSSEQLQQRLAEARRRILVLTFVLLMVGV
                     AGIWVLSN"
     gene            complement(1137..>1419)
                     /locus_tag="PK112_08635"
     CDS             complement(1137..>1419)
                     /locus_tag="PK112_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12149.1"
                     /translation="EDVLRLQEALAADIAGGLRLTLGRDGAARLSVSGTKDPEAWQLY
                     LEGLRFMRNQEYQKAIDAWEKVLERYPGNEATLDNIEQARLRLKSNNQD"
BASE COUNT          233 a          471 c          461 g          254 t
ORIGIN      
        1 agttcgacag gacccagatc ccggccaccc ccaccatcag caggacgaaa gtgaggacga
       61 gaatgcgccg gcgcgcctcg gccagccgct gctggagctg ctcggaggaa tacacggcgt
      121 ggaccgtccc gaacgaccgg tccccctgcc ggatcggcag catcaggtag ctcagcgcac
      181 gcccgttctc ggagaaccgg aagggcaccc cgacgcgggc cgcgtgcacc tccggcgggg
      241 gcgcgtacgg tttgcggatg ttgtggatgt cgtcggagtg ggccaggatc gttccggccg
      301 tgtccgtgag caccacctgc cgcaggatgc cggcgttgga gcgcacggcg ctcacgatca
      361 actcgtcgaa ctcgacgtcc gaccgccggt tgatcacgta cccggcggcc tgatccccga
      421 tcgtgttcac cagcgacgcc accgtctcgt cgaggtgcga tttcatctgc cgcgtcgtct
      481 ggctgtcgaa gtagtagtag gcgcccccaa cgatgaccag cacgatgagg aaggtccaga
      541 ccgaaaactt cgcccgcagc gtggccatgt ggcggaggaa ggggatgctc tcgctggcca
      601 ccggctgcgc ctgcttgatc atccgcagtt cattgtaccc cgccgaggag atgtactcca
      661 ccgagtccat gatcttcttg atcatgtaga accccagccc ccccttgcgg cccgattcga
      721 cgaggcgctg caggtcgagc ttgccgtccg actccggctg gtacgaccgc ccggtgtcga
      781 tgagcgagaa gacgatcatt ttcttgtaga tcacgatgcg gagccggatg gtccccttct
      841 cgtagaggta ggcgtggcgg atgatgttgg tggccccctc ctcgatcgcc agcagaatgg
      901 gcgagacctc tttcctggac agcccggccg agaggcaggc ctcgcgggtg atgcgctgga
      961 ggctgtcgag gtatttctcc tccgccacga cttcggcgtt gatctctttg acgggacgac
     1021 ggaacatagg tcgatagtat gcgatggacg ggccggcgtc catttaaaaa acgcgcgtcc
     1081 cggcgggacc gcgggtcggg gcgccgcgcc gtgccgcccc ggcagccgat ggccgcctag
     1141 tcctggttgt tcgacttgag gcggagtcga gcctgctcaa tgttgtccag cgtggcttcg
     1201 ttgcccggat accgctccag caccttctcc cacgcgtcga tcgctttctg gtactcctgg
     1261 ttccgcatga agcgcaggcc ctcgaggtac agctgccagg cctcgggatc cttcgtcccg
     1321 gacacggaga gccgggcggc cccgtcccgt cccagggtga gcctcagacc gcccgcgata
     1381 tcggcggcga gggcctcctg cagccgaagc acgtcctcc
//
LOCUS       DAOOKS010000574         1415 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_121092_length_1415_cov_2.464706, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000574 DAOOKS010000000
VERSION     DAOOKS010000574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1415
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121092_length_1415_cov_2.464706"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            177..815
                     /locus_tag="PK112_08640"
     CDS             177..815
                     /locus_tag="PK112_08640"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPC12150.1"
                     /translation="MKRNPAIPHHLREAIPPPDERDEERVKELIREIKRGNQLAFAEL
                     VRRYRVQVAALAYKMVGDYDEAADIAQNVFVKMSRNIWRYDERRKFYTWLYRITVNAS
                     IDYIRKHSRHRHEPLENFENVVEQADRRPDASYHRRQIDDYIREATASLTDKQRSAFV
                     LRDVEGCRIDDVADIMNMPEATVRWYLHRARTKIRRELLRKCPHLLALFGIR"
     gene            912..>1415
                     /locus_tag="PK112_08645"
     CDS             912..>1415
                     /locus_tag="PK112_08645"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HPC12151.1"
                     /translation="MAQTEELQVNIDGMHCAGCVANIERGLGALAGVAECRVNLATRS
                     AVVAYDRDETSAGAILRTIEKLGYGARVGAPDVLTANRKELSAAIRRFWPALGLSLPL
                     MALAMGPMVGLGGILAPAADGIAQALLAGGILFGAGRAILGDAFAQARRRAANMNTLI
                     ALGTLTAY"
BASE COUNT          270 a          439 c          485 g          221 t
ORIGIN      
        1 cctgaacgac aacagttgat cggcggtccc cggcaagcga ggaggcgtcc ggagccgggc
       61 gccttttttc gtggccgatt gaaccgggaa ccgtcccctc cccgctcctt cgccggcact
      121 catggaactt tggccggggc atgcggttat acctgacaaa gcgtacggag caaggcatga
      181 aacgaaatcc ggcgatcccg caccatctcc gcgaagcgat ccccccgccg gatgaacgcg
      241 atgaagagcg ggtcaaagaa ctaatccggg aaatcaagcg cggcaaccaa ctggcctttg
      301 ccgaactggt gcgccgctac cgggtgcagg tcgcggcgct cgcctacaag atggtcggcg
      361 actacgacga ggcggccgat atcgcgcaaa acgtgttcgt caaaatgtcc cgcaacatct
      421 ggcggtacga tgagcggcgg aagttctaca cctggctgta ccggatcacg gtcaacgctt
      481 caatcgacta tatccgcaag cacagccgcc accgccacga gccgctggag aatttcgaga
      541 acgtggtcga gcaggcggac cgacgcccgg atgcatcgta ccaccggcgg cagatcgatg
      601 attacatccg ggaggcaacc gcgtcgctca ccgacaagca gcgctcggcg ttcgtgctgc
      661 gcgatgtgga ggggtgccga atcgacgacg tcgccgacat catgaacatg ccggaagcaa
      721 ccgtgcgctg gtacctgcac cgggcgcgga cgaagatccg gcgggagctg cttcggaagt
      781 gcccgcacct gctcgcgctg ttcgggatca gatgacgcgc ccgcgagcct taccgtacct
      841 tttctgttgg cgaacaacga tcttaaggcc ggagagcggg cttttggccc gaggtttgga
      901 aggaccggtg gatggcccag accgaagaac ttcaggtaaa catcgacggg atgcactgcg
      961 ccgggtgcgt cgcgaacatc gagcgcggtc tcggagcgct ggcgggggtg gcggagtgcc
     1021 gggtgaatct ggcgacccgc tcggcggtcg ttgcgtacga ccgggacgag acgagcgcgg
     1081 gggcgattct ccggacgatc gagaaactcg gctacggagc gcgggtcggc gcgccggacg
     1141 tactcaccgc caaccggaaa gaattgagcg ccgcgatccg gcggttctgg ccggccctgg
     1201 ggctgagtct ccccctgatg gcgctggcga tggggccgat ggtcggtctc ggcgggatcc
     1261 tggcgccggc ggccgacggg atcgcgcagg cgctgctggc ggggggaatt ctgttcggcg
     1321 ccggtcgggc gatactcggc gacgctttcg cacaggcccg gcgaagggcg gccaacatga
     1381 acacgctgat cgcgctcggc acactcaccg cctac
//
LOCUS       DAOOKS010000575         1408 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_122012_length_1408_cov_2.972653, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000575 DAOOKS010000000
VERSION     DAOOKS010000575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122012_length_1408_cov_2.972653"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(154..>1408)
                     /gene="metG"
                     /locus_tag="PK112_08650"
     CDS             complement(154..>1408)
                     /gene="metG"
                     /locus_tag="PK112_08650"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF020892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HPC12152.1"
                     /translation="QVAYVWVDALSNYISAVGYGDDPAAFAKWWTNAEVVHLMAKDIL
                     KFHCLYWPAMLMAAGVRLPDTMFLHGFFTVDGEKMSKSLGNQIDPNAMVAQFGADGTR
                     YLLLTQYPFGVDGDIQAQRFAAQYNADLANDLGNLVSRVVKMVQTNFEGRLPGPHREI
                     AGLQELMEAAEAAPAAAYEHIRNFRIGQAVAEGMNLVRAANKFFNDRVPWQLARDGRR
                     DEMGGILYACCEVIRIVSIILAPVMPNKMRAVRGIFGLDDGTLTLDAARVFFALEPGT
                     PVAFGEAVFPRIAPPQEAPPATAAAPPPAEGSADGLIDIADFGRVQLRVAEVLRAERV
                     PGADRLLRLQISLGDETRQIVAGVAEHYTPEQITGRRIVVVANLKPATIRGLESRGML
                     LAAKKGKVLRLVVPDGDLPPGATIS"
BASE COUNT          223 a          515 c          456 g          214 t
ORIGIN      
        1 gctgctcggc cagcgcgacc gagcgctccg aggcggccag atccaccccg atgttgatga
       61 cagtgtggac gccggcggcc cgcgcggcgg ccagcgcctc ctcgcgccgg ccctcgaaag
      121 ccgcgaaatc gagatggcag tgggagtcga tcatcagctt atcgtggcgc ccggcggaag
      181 atccccgtcg ggcacgacca gccgaagcac tttccccttt ttcgccgcca gcagcatccc
      241 ccgcgactcc agcccccgga tggtcgcggg cttgaggttg gcgacgacca cgatgcgcct
      301 gcccgtgatc tgctccggcg tgtagtgctc cgccaccccg gcgacaatct ggcgggtctc
      361 gtcgccgagt gaaatctgca ggcggaggag ccggtccgct ccggggaccc gctcggcgcg
      421 gagcacctcg gccacccgga gctgcacgcg gccgaagtcg gcgatatcga tgaggccgtc
      481 ggccgagcct tccgcgggcg gcggcgcggc cgcggtcgcg ggcggcgctt cctgcggcgg
      541 cgcgatccgc gggaagaccg cctcgccgaa ggccactggc gttcccggct ccagcgcgaa
      601 aaacacccgc gcggcgtcga gcgtgagcgt cccgtcgtcc agtccgaaaa tgccgcgcac
      661 cgcgcgcatc ttgttgggca tcaccggggc gaggatgatc gagacaatcc ggatcacctc
      721 gcaacaggcg tagaggatcc cgcccatctc gtcgcgccgc ccgtcgcggg caagctgcca
      781 cggcacgcgg tcgttgaaga acttgttggc cgcccgcacg aggttcattc cctcggcgac
      841 agcctgcccg atccggaaat tccggatgtg ctcgtaggcc gcggccggcg cggcctcggc
      901 cgcctccatc agctcctgca gcccggcaat ctcccggtgc ggacccggca gccggccctc
      961 gaaattcgtc tgcaccatct tgaccacccg cgacacgagg ttccccagat cgttcgcgag
     1021 gtcggcgttg tactgcgccg caaaccgctg ggcctggatg tccccgtcga ccccgaacgg
     1081 gtactgagtg agcagcaggt accgggtgcc gtcggccccg aactgggcga ccatggcgtt
     1141 cgggtcgatc tggttcccga gcgacttcga catcttctcg ccgtcgaccg tgaaaaaccc
     1201 gtgcaggaac atcgtgtccg gcaaccgcac tcccgccgcc atcagcatcg ccggccaata
     1261 gaggcagtgg aacttgagga tgtccttggc catcaggtgc acgacttcag cgttggtcca
     1321 ccacttcgcg aacgccgccg gatcgtcgcc gtacccgacc gccgagatgt agttcgacag
     1381 cgcgtccacc cacacgtagg ccacctgc
//
LOCUS       DAOOKS010000576         1403 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_122687_length_1403_cov_3.442878, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000576 DAOOKS010000000
VERSION     DAOOKS010000576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122687_length_1403_cov_3.442878"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1229
                     /locus_tag="PK112_08655"
     CDS             <1..1229
                     /locus_tag="PK112_08655"
                     /inference="COORDINATES: protein motif:HMM:NF024764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPC12153.1"
                     /translation="VSQRLTVGGLLLLVAGWAVVGVRQSHLFFRYSLVPPFEDAFTIG
                     GYTLFSPAHLADLLNLLVLLLPPVGLLVLVIVSAARSRRLAARDAVFLIVLSLSALGA
                     AFIFDPKLGMPRDWDLFAFAGIAPAVLLYVLTLDPQSPLPGGLRVAAMAVVLSLFALG
                     GRLVAANDPPIQIARFNDYLTVDPERDRNARYLLLTYYKDRGDSAAADRVRAEWRAAF
                     PLRDKLDEAQRLHREEKLPEATALFEQIVREDPTYADAWAGLGWMMVRYRDWDSAIAC
                     LDMSLGLNPYHAAACSNRGLAHLQLGHAQQAARDFERSLSIDPNVPAVMYNLAVAYLR
                     CRRYEDAGRVIARMAAMPDMLAGEHLKLFEWYATYGPLPAAVAQLRFAIDKGADSAYV
                     QYLLQRFPDAAKAFRR"
BASE COUNT          194 a          522 c          411 g          276 t
ORIGIN      
        1 gggtatcgca acgcctgacg gtgggcggtc tgcttctcct ggtcgctggc tgggctgtcg
       61 tcggcgtgcg gcagtcgcac ctgtttttcc gctactcgct cgtcccgccg ttcgaggatg
      121 ccttcacaat cggcgggtat actctctttt cacccgccca cctggccgac ctgctgaacc
      181 tgctggtgct tctgctgcct ccggtgggtc tgctcgtcct cgtcatcgtg agcgccgccc
      241 gctcccgccg cctggccgcg cgcgacgccg tcttcctcat cgtgctctcc ctctcggcgc
      301 tcggcgcagc cttcattttt gacccgaaac tcggcatgcc ccgtgactgg gacctcttcg
      361 ctttcgccgg aatcgccccc gccgtgctgc tctatgtcct gactctggat ccccaatccc
      421 ccctgccggg cggcctgcgt gtcgccgcga tggctgtcgt cctttcgctc tttgcgctgg
      481 gcggccggct ggtcgccgcc aacgatcctc ccatccagat cgcccgtttc aacgactacc
      541 tgacggtcga cccggaacgc gatcgcaacg cccgctatct cctgttgacc tactacaaag
      601 accgcggcga cagcgccgcc gccgaccggg tgcgcgccga gtggcgcgcc gctttccccc
      661 tccgcgacaa actggacgag gcgcagcgcc tccaccgtga ggagaagctg ccggaagcga
      721 ccgccctgtt cgagcagatt gtccgcgagg atccgacgta tgccgatgcc tgggccgggc
      781 tggggtggat gatggtgcgc taccgcgatt gggacagcgc cattgcctgt ctcgacatgt
      841 cgctcggtct caatccctac cacgccgccg cctgtagtaa ccgcggcctg gctcatctgc
      901 agctcggcca tgcccaacag gcggcccggg atttcgagcg gtccctgtcg atcgacccca
      961 acgtccccgc cgtcatgtac aacctcgccg tcgcctacct gcgctgccgc cgctacgagg
     1021 acgccggcag agtgatcgcc cgcatggcgg cgatgccgga catgctggcc ggcgaacacc
     1081 tgaaactgtt cgaatggtat gccacctatg gtcctctccc ggcggcggtc gcgcagctcc
     1141 gcttcgcgat cgacaaggga gccgattccg cgtacgtcca gtacctgctg cagcgttttc
     1201 cggacgccgc gaaggccttc cggcggtagc cgcggccccc gaatccggtt gaccggagcc
     1261 ggttgcggtg ctacctttcg cccctatgac cagtcctgcc gaccataccc ggaccgaaaa
     1321 actcctcctt gaggcggccc ggatcttcaa cagcacgctc gaatacgaga gcctgattgc
     1381 cgaggtgctc cgcctggtca aca
//
LOCUS       DAOOKS010000577         1393 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_124171_length_1393_cov_2.584454, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000577 DAOOKS010000000
VERSION     DAOOKS010000577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124171_length_1393_cov_2.584454"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="PK112_08660"
     CDS             <1..814
                     /locus_tag="PK112_08660"
                     /inference="COORDINATES: protein motif:HMM:NF012432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HPC12154.1"
                     /translation="DRARQRVRAAADGVAVGKISGAVGNFAHLDPKIEAMVCRRLGLK
                     PAPVSTQVVQRDRHAALVTALALLASSLEKFATEIRLLQRTETGEMMEGFSRKQKGSS
                     AMPHKKNPITAERICGIARLLRGYALSAMENIPLWHERDIAHSSVERVILPDACIITD
                     YGLQKFIEILNGLVIDARRMRENIYYRGGVVFSQQLLLKLAGPVGSRDTAYRIVQRNA
                     MKALETGESFRDLVRQDIEIRRYLSEKDIDRSFDLEYYTKNVDRIFERVFGK"
     gene            811..>1393
                     /locus_tag="PK112_08665"
     CDS             811..>1393
                     /locus_tag="PK112_08665"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF014696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HPC12155.1"
                     /translation="MIVRDGWPFLVIGAALVLATAAGAVRWSSLGLLAAAGLCALLTL
                     FTAYFFRNPPRVVPDDPRAVVAPADGKVVLITPLEDHPALGGPATRISIFLSVFDVHI
                     NRIPTAGAVDYVKYNPGRFFAAFADKASELNEQTEVGLTTPGGARLAVAQIAGLIARR
                     IVCRLRAGDTVRTGAPFGMIRFGSRTDLTIPAAW"
BASE COUNT          223 a          471 c          468 g          231 t
ORIGIN      
        1 tgaccgggcc cgccagcggg tgcgcgcggc ggccgacggg gtggcggtcg gcaagatctc
       61 cggggcggtg gggaatttcg cccacctcga cccgaagatc gaagcgatgg tgtgccggcg
      121 gctcggcctg aaaccggcgc cggtctcgac ccaggtggtg cagcgcgacc gccacgccgc
      181 gctggtgacg gcgctggcgc tcctcgcctc gtcgctggag aagtttgcca ccgagatccg
      241 tctcctccag cgcaccgaga cgggggagat gatggagggg ttcagcagga agcagaaggg
      301 gtcatcggcg atgccccaca agaagaaccc gatcacggcc gagcgcatct gcggcatcgc
      361 ccgcctgttg cgcggatacg cgctgagcgc gatggagaac atccccctct ggcacgaacg
      421 cgacatcgcc cactcctcgg tcgaacgcgt gattctcccc gacgcctgca tcatcaccga
      481 ctatggtctg cagaaattca tcgagatcct caacggtctc gtgatcgacg cgcggcgcat
      541 gcgcgagaac atctactacc gcggcggcgt cgtcttctcg cagcagctcc tcctcaagct
      601 cgcggggccg gtcggctcgc gcgacaccgc ctaccggata gtgcagcgca acgcgatgaa
      661 ggcgctcgag acgggagagt cgttccgaga cctggtcagg caggacatcg agatccgccg
      721 gtacctctcg gagaaagaca tcgaccggag tttcgacctg gagtactata cgaaaaacgt
      781 ggaccgcata ttcgaacggg tgtttgggaa gtgattgtgc gcgacggttg gccgtttctt
      841 gtgatcggag cggcgctcgt gctggcgacg gcggcggggg cggtgcgctg gagcagcctc
      901 gggctgctgg cggcggccgg cctgtgcgct ctgcttaccc tgttcaccgc ctatttcttc
      961 cgcaaccccc cgcgggttgt tcccgatgac ccgcgggcgg tggtcgcccc cgccgacggc
     1021 aaagtcgtgc tcatcactcc cctggaggat caccccgccc tcggcggccc ggccacgcgc
     1081 atctcgattt tcctgagcgt gttcgacgtc cacatcaacc gtatcccgac cgccggcgcg
     1141 gtcgactacg tgaagtacaa ccccggtcgg tttttcgcgg cctttgccga caaagcatcc
     1201 gaactcaacg agcagaccga ggtcggcctg accacgccgg ggggggcgcg gctggcggtg
     1261 gcgcagatcg ccgggctgat cgcccgccgc atcgtctgcc ggctccgcgc cggggacacg
     1321 gtgcgcaccg gcgcgccgtt cggcatgatc cgtttcgggt cgcggaccga tctcacgatt
     1381 cccgccgcct ggg
//
LOCUS       DAOOKS010000578         1391 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_124434_length_1391_cov_2.964072, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000578 DAOOKS010000000
VERSION     DAOOKS010000578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124434_length_1391_cov_2.964072"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..820)
                     /locus_tag="PK112_08670"
     CDS             complement(<1..820)
                     /locus_tag="PK112_08670"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HPC12156.1"
                     /translation="MRTVEHILDLVGHTPLVRLRTGMTDPGPRAYVKLEFFNPTGSVK
                     DRMAYYIITKALRDGRLKPGDTVIDNTSGNTGSAVAMVCSVLGLRAVLTTPVKTSQEK
                     IDLIKSFGAEVIVTRETEDHADPEGAYMVARRLAREHGYFDLDQYNSQENVEAHYRLT
                     GPEIWDDTEGRLTHFVAGIGTGGTFSGVARFLKEKNPAIKAIAVDPEGSVFAAYIRSG
                     RTTTPAAYKIEGIGSDVITEALHPQYIDEVITVSDRDAFQRARLIARTEGISGGG"
     gene            1004..>1391
                     /locus_tag="PK112_08675"
     CDS             1004..>1391
                     /locus_tag="PK112_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12157.1"
                     /translation="MRSLGILCCLILAAACGLAGCSKDDDIVELEREVMAQQGQTDSH
                     PAAVSDVPVLPAAASAPDSVRPELNAGAIPDEPPDEPLNETPVEAQDVAPETTPDQPL
                     TPVDLPVPPAGDGFVVQLAGCEDPDYA"
BASE COUNT          217 a          479 c          429 g          266 t
ORIGIN      
        1 acccgccgcc ggagatgccc tcggtccgcg cgatcagccg cgcccgctgg aaagcgtcgc
       61 ggtcggaaac ggtaatgacc tcgtcgatat attgcggatg gagcgcctcg gtgatcacgt
      121 cggagccgat cccttcaatt ttgtacgccg cgggggtggt ggtccgcccg ctgcggatat
      181 aggcggcgaa caccgaaccc tccgggtcga ccgcgatcgc cttgatcgcc ggattcttct
      241 ccttgaggaa ccgcgcgacg ccggaaaacg tccccccggt tccgatcccg gccacgaagt
      301 gggtcagccg gccttcggtg tcgtcccaga tctccggacc ggtcaggcgg tagtgggcct
      361 cgacattctc ctggctgttg tactggtcga ggtcgaagta cccgtgctcg cgggccagcc
      421 tccgggccac catgtaggct ccctccgggt cggcgtgatc ctccgtctcc cgcgtcacga
      481 tcacctcagc gccgaacgac ttgatcaggt caatcttctc ctggctcgtc ttcaccggcg
      541 tcgtcagcac ggcccgcagt ccgagcacgg agcagaccat cgccaccgcc gagccggtgt
      601 tgccggaggt attgtcgatc acggtgtcgc ccggtttcag ccggccgtcc cgcagcgcct
      661 tggtgatgat gtagtaggcc atgcggtctt tcaccgagcc ggtcgggttg aagaactcga
      721 gcttcacgta cgcccgcggt ccgggatcgg tcattccggt ccgcagccgc accagcgggg
      781 tgtggcccac caggtcgaga atgtgttcta ctgtcctcat acgcctcttg cttttactcg
      841 tcaagcgtag gcatttctta tacaaatttc aactattttc atcgcccccc ggacaaacgc
      901 tttttcgttg acaatcccgg ccgcgcgccc tagtatcggc cggaagcatc gcgtagacct
      961 aactgcggcg ccccggcgcc ggcgggactg atttggaggt actgtgagat ccctggggat
     1021 actttgctgc ctgattctgg ccgccgcctg cgggctcgcc ggctgttcga aagacgatga
     1081 cattgtcgag ctggagcggg aggtgatggc gcaacaggga cagaccgact cccaccccgc
     1141 ggcggtttcc gacgtccccg tgctgccggc ggcggcctca gcccccgatt cggtccgccc
     1201 cgaactgaac gccggcgcga ttcccgacga gcccccggac gagcctctca atgagacgcc
     1261 ggtcgaggcg caggatgtcg caccggaaac gaccccggac cagcccctga ccccggttga
     1321 cctcccggtc ccgcctgccg gcgacggttt cgtcgtccag ctcgcggggt gcgaagatcc
     1381 cgattacgcc g
//
LOCUS       DAOOKS010000579         1391 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_124457_length_1391_cov_2.576347, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000579 DAOOKS010000000
VERSION     DAOOKS010000579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124457_length_1391_cov_2.576347"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1391)
                     /locus_tag="PK112_08680"
     CDS             complement(<1..>1391)
                     /locus_tag="PK112_08680"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HPC12158.1"
                     /translation="AKSIDLGGKCAIPIDRHSAYFVNFPPPGSIVNYSAADILSDTFD
                     RRQLAGKLVLIGLDDVTSRETFTTPVAANMTKLQVQAAAAENIVNADFLAVSHSTGTT
                     LLILLAVGIVFAFLLPQVSVMYRFIILLIALVILINANYFLFSAFRVVPETVYIALEL
                     LLFLAATPLLDSQLIRGAEPAKARDEFDDIGALRNVERRTVRPEDLAHAPVRTVQENP
                     LDHAPTTAIDYRPARTSTAPAATEALPVDHAVIDIDRPADEASAPVAEEQHFDASLIS
                     DDDLEIIEAGHPAPADRSAGAPMSETVYDPPADASGEWDLANQTPGGTPRPANLGRYQ
                     ITGTLGRGAMGLVYKGIDPAINRPVALKTIRLDFVNDPEEMKELKERLYREAQAAGKL
                     SHPNIVTIYDVGSEGPLQYIAMEYLEGRTLEEIIKKKTKFNYRIFAEIIIQVCSALQA
                     AHEQGIVHRDIKP"
BASE COUNT          204 a          369 c          554 g          264 t
ORIGIN      
        1 ccggcttgat gtcgcggtgg acgatcccct gctcgtgcgc ggcctggagg gccgagcaga
       61 cctggatgat gatttcggcg aagatgcggt agttgaattt ggtcttcttc ttgatgatct
      121 cctcgagggt gcggccctcg agatactcca tggcgatgta ctggaggggg ccctcggaac
      181 cgacgtcgta gatggtgacg atgttggggt gggagagttt gccggcagcc tgggcctcgc
      241 ggtagaggcg ctctttgagt tccttcattt cctcgggatc gttgacgaaa tcgaggcgga
      301 tggtcttgag ggcgacggga cggttgatcg ccggatcgat gcctttgtaa acgaggccca
      361 tggcgcccct gccgagcgtg cccgtgatct ggtagcgccc gaggttggcg gggcgggggg
      421 tgccgcccgg ggtctggttg gccaggtccc actcgccgga ggcgtcggcg ggcgggtcat
      481 agacggtttc gctcattggg gcgccggccg accggtcggc gggggcggga tgcccggcct
      541 cgatgatctc caggtcgtcg tcactgatca gggacgcgtc gaagtgctgc tcctccgcaa
      601 ccggggcgga ggcttcgtcg gcggggcggt cgatgtcgat cacggcgtgg tcgaccggca
      661 gggcctcggt ggccgcgggg gcggtagagg tgcgggccgg ccggtagtcg atggcggtcg
      721 tgggcgcatg gtccagcggg ttttcctgca cggtgcggac cggcgcgtgc gcgagatcct
      781 cgggccgtac ggtgcggcgc tcgacgttcc tcagcgcgcc gatgtcgtcg aattcgtcgc
      841 gcgccttggc cggctccgcg ccgcggatga gctgcgagtc gagcaagggg gtcgcggcca
      901 ggaagagcag gagttcgagg gcgatgtaga cggtttccgg cacgacgcgg aatgcgctga
      961 agaggaagta gttggcgttg atgaggatga ccagcgcgat caggaggatg atgaaccggt
     1021 acatcacgga cacctggggc aggaggaagg cgaagacgat gccgacggcc aggaggatga
     1081 gcagggtggt gccggtgctg tggctcacgg cgaggaagtc ggcgttgacg atgttctcgg
     1141 ccgcggccgc ctggacctgg agcttggtca tgttggcggc gaccggcgtg gtgaacgttt
     1201 cccgcgaggt cacgtcgtcc agaccgatca gcacgagttt gccggctagt tgcctgcggt
     1261 cgaaggtatc gctgaggatg tcggccgcgg agtagttgac gatgctgccg ggcggaggga
     1321 agttgacgaa gtaggcgctg tgacggtcga tcgggatcgc gcactttcct ccgaggtcga
     1381 tgctcttcgc c
//
LOCUS       DAOOKS010000580         1389 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_124696_length_1389_cov_2.961019, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000580 DAOOKS010000000
VERSION     DAOOKS010000580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124696_length_1389_cov_2.961019"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="PK112_08685"
     CDS             <1..632
                     /locus_tag="PK112_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12159.1"
                     /translation="SAVGKLPARIFTWIDGSIVGMYLLATMIAGIMVRKYVSRVDHFL
                     VAGREMNVFLGIASLAATDMNRDARTGTIEDLVLLMRAVLGEYVPDPGMFLNRDAPAE
                     SPFAPPPPKDSRATLRLEENELAVEAEDSLVAARFVFRGRIVPHVYNTRLDVYSTYVN
                     GQTRVLVYGFFSGASFGSGPFLYWEGSGRLTGFSLATVSGGRVEVRIRR"
     gene            746..>1389
                     /locus_tag="PK112_08690"
     CDS             746..>1389
                     /locus_tag="PK112_08690"
                     /inference="COORDINATES: protein motif:HMM:NF015039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HPC12160.1"
                     /translation="MRPLILSTLAALWIGGPSVWAAEGGLDPALVARIRADYDATGFS
                     PATVNAVTNNDLRALALSREKVTAFDDLFNVRLKETGITNQAGSGRCWLFAGLNVFSP
                     RVCTKLDLSKFQFSQPYLAFWDKMEKANFFLEEMIRLRERPLYDRELAVALDGPFGDG
                     GWWHYATALIEKYGVVPLTAMPETKQSGATGTVNSLAERKLRAFAAEIRRMAAGG"
BASE COUNT          229 a          446 c          472 g          242 t
ORIGIN      
        1 tctccgccgt cggtaaattg ccagcgcgga tattcacctg gatcgacggc agcatcgtcg
       61 ggatgtacct gctggccacg atgattgccg gcatcatggt gcggaagtac gtctcccgcg
      121 tcgatcactt tctggtcgct ggccgcgaaa tgaacgtctt tctggggatc gcctcgctgg
      181 cggccaccga catgaaccgc gacgcgcgga cgggaacgat cgaggatctc gtgctgctga
      241 tgcgggcggt gctgggcgag tatgtgccgg acccggggat gtttctcaac cgcgacgcgc
      301 ccgcggagag cccgttcgcg ccgccccccc cgaaagacag ccgggcgacc ctgcggctcg
      361 aggagaacga gctcgcagtg gaagccgagg actcgctcgt ggcggcccgg ttcgtgttcc
      421 ggggacggat cgtccctcac gtctacaaca cccggcttga tgtgtacagc acctatgtca
      481 acggacaaac gcgcgtgctc gtctacgggt tcttcagcgg ggcgtccttc ggcagcgggc
      541 cgtttctcta ttgggagggg agcggccggc tgacgggttt ctcgctggcg accgtgtcgg
      601 ggggccgggt cgaggtgcgc atccgccgat aatcccgccc gcggcgacaa tcgccgttgc
      661 ccgctccgcg caaatccgct accttgccgg gaaaatcgcc ggccaacggt cgggaatatc
      721 gacgctcaga agggagagac aacacatgcg cccgctcatc ctcagtaccc ttgccgccct
      781 gtggatcgga ggcccgtcgg tgtgggccgc ggagggcggc ctggatcccg ccctggtggc
      841 caggattcgc gccgactacg acgcgaccgg gttttcgccg gccaccgtca acgccgtcac
      901 caacaacgac ctcagagcgc tcgccctgag ccgggagaaa gtgaccgcct tcgacgacct
      961 gttcaacgtg cgcctcaagg agaccgggat caccaaccag gccggctcgg gccggtgctg
     1021 gctttttgcg ggactgaatg tcttctcccc ccgggtctgc accaagctcg acctgtcgaa
     1081 attccagttc tcccagccgt atctcgcctt ctgggacaag atggaaaagg cgaacttctt
     1141 tctcgaagag atgatccgcc tgcgggagcg gccgctgtat gaccgggaac tggcggtggc
     1201 gctcgatggt cctttcgggg acggcggctg gtggcactat gccaccgcgc tgatcgagaa
     1261 atacggcgtg gtgccgctga cggcgatgcc ggagacgaag cagtcgggcg cgacggggac
     1321 ggtcaattcg ctggccgagc gcaagctgcg cgccttcgcg gcggagatcc ggcggatggc
     1381 cgccggggg
//
LOCUS       DAOOKS010000581         1386 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_125122_length_1386_cov_3.039820, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000581 DAOOKS010000000
VERSION     DAOOKS010000581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1386
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125122_length_1386_cov_3.039820"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1325)
                     /gene="dxs"
                     /locus_tag="PK112_08695"
                     /pseudo
     CDS             complement(1..1325)
                     /gene="dxs"
                     /locus_tag="PK112_08695"
                     /inference="COORDINATES: protein motif:HMM:NF024688.2"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
BASE COUNT          214 a          493 c          442 g          237 t
ORIGIN      
        1 tcccgggcga cattttccag aagggtcgcg accgcttccg catcgagccc gatctcggcc
       61 aggagctcct cccggtcccc gtgggtcaca aaccggtcgg ggataccgag ggcgcggaac
      121 tggccgtccc acccgcgctg gaggaggaac gcgccgaccg cctcgccgaa cccgccccgc
      181 agttgtccct cctccatggt gatgatcgcc cgcgcgtgat cggcgatctc cagcagcatg
      241 gcttcgtccc acggtttgac gaagcgggcg ttgatgatgc ccacccggtc agcgccctcg
      301 cgcatcctcg ccgccgcctc ctgcgcctgc gccaccatag tcccgaccgc cagcacggcc
      361 acctcgcggc ggggagttac gtactcccac ttcccccact cgagggggtc gatttcgggg
      421 cgcatcgcgg tcggcacggc gccgcgcggg tagcggatgg ccaccggccc cggcaggtcg
      481 tggccggccg tgtagtggag catcgcccgg agctcgttcc cgtctttcgg cgccgccacc
      541 gcgacattgg gcaccgagcc gagatagcac aggtcgaaca cgccgtggtg cgtggggccg
      601 tcgtttccca ccagcccggc gcggtcgagg cacatgacca ccggcaggtt ttgcagggcg
      661 atgtcgtgca ccacctgatc gtaagtgcgc tgcaggaagg tcgagtagat cgcgaggtac
      721 ggcttgatgc cctcggcggc cagtccggcg gcgaaggtcc cggcgtgctc ctccgcgatc
      781 ccgacatcga aaaaccgctc ggggtaggcg gcggcaaaat cgaccagccc cgtcccgtcg
      841 cacatggcgg cgctgatcgc cgccacccgc cggtctttgg cccccagttc gaccatcgtg
      901 tcgccgaaca ccttcgtgaa cgtcggcagg cccgccgatt tcgccgccgc cttgcccgtc
      961 accttgtcga actgcccgac cccgtggaac cgcgagggat ctccctcggc cgggcggaac
     1021 ccccgcccct tctgcgtcac cacgtgcaac aggagcggtc cgcggatgtt ctggagatcg
     1081 cgcaggcgct tgatgagcag ttcgaggtcg tggccgtcga tcggcccgaa atagcggaag
     1141 ccgagttttt cgaagagcat gcccggggtg atcagtccga gcacggagtc ctcgatccgc
     1201 gaaatggcct cgcggatccg gtcgcgccgc ttgaaccgcc cggtcagttc ccagatttcg
     1261 ttgcgcagcc ggttgaactt ctcgtccgtc atgatgctcg tgaagtagcg ctagatcgcc
     1321 cccacgttct tcgagatcga ccaggtgttg tcgttgagaa cgaccagcag atccttcttg
     1381 agcgat
//
LOCUS       DAOOKS010000582         1380 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_125968_length_1380_cov_3.381132, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000582 DAOOKS010000000
VERSION     DAOOKS010000582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125968_length_1380_cov_3.381132"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1064
                     /locus_tag="PK112_08700"
     CDS             <1..1064
                     /locus_tag="PK112_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12161.1"
                     /translation="ALLLPPKDVTLPTPGAGRLEDVVSVTKGLNLPVMVTPSDVYARM
                     LKSRTIAREIVERFDLMALYKEDNFVETYDVLMDRARFRVTEEGLLEISVEDRNAARA
                     AAIANAFVDVLDSINREIVTGRVRQNRTFVQERLAVAKVELDSARAQFREFQMRNRAI
                     DFDEQTRLAIDQAARLKSDLASLDIDIAMKEQTLGADNTDLRELKRRRATVQDRLDQL
                     ERSNPDSSFFSLPVSAIPTLRGQYEVLYSRVRVAESIYRILLEQLEEVKYQEQEKQPV
                     LSVLDRAVPPEIKSRPQRSIIVLAAFALAVLAAVLLAAAVDYVDRMRTASPENYRRVQ
                     MFAQAWFGWLPGLKRRPAE"
     gene            1136..>1380
                     /locus_tag="PK112_08705"
     CDS             1136..>1380
                     /locus_tag="PK112_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12162.1"
                     /translation="MTSSEISPEARIDPTAHIGRFCVIGAQATVGAGCVIGHHVVIHE
                     DTAVGDNVRIDDGAVLGKRPMRAANSARVAADGAAGSP"
BASE COUNT          234 a          420 c          513 g          213 t
ORIGIN      
        1 cggcgctgct gttgcccccg aaagacgtca cgctgccgac gccgggtgcg gggcgcctcg
       61 aggacgtcgt gtcggtcacg aagggtttga acctgccggt gatggtgacg ccgtcggatg
      121 tctacgcgcg catgctgaaa agccggacga tcgcccggga gatcgtcgaa cgcttcgacc
      181 tcatggcgct ctacaaggaa gacaatttcg tcgagaccta cgacgtgctg atggaccggg
      241 cgagattccg ggtgacggag gagggactgc tggagatttc ggtggaggac cggaacgcgg
      301 cgcgggcggc cgcgattgcg aacgccttcg tcgacgtgct cgatagcatc aaccgggaga
      361 ttgtgacggg gcgggtgcgg cagaaccgga cgttcgtgca ggaacggctg gcggtcgcga
      421 aggtcgagct ggattccgcc cgcgcccagt tccgggagtt ccagatgcgc aaccgggcga
      481 tcgatttcga tgagcagacg cggttggcga tcgaccaggc ggcccggctc aagagcgacc
      541 tggcctccct cgacatcgac atcgccatga aggagcagac gctgggggcc gacaacaccg
      601 acctgaggga gctgaagcgc cggcgggcca ctgtccagga ccgtctcgat cagctcgagc
      661 ggagcaaccc cgacagctcg ttcttctcgc tcccggtgtc ggccattccg acgctccgcg
      721 ggcagtacga agtgctctac agccgggtgc gggtggcgga gtcgatttac cggattctcc
      781 tggagcagct cgaggaagtg aagtaccagg agcaggagaa gcagccggtt ctgtcggtgc
      841 tggaccgggc ggtcccgccg gagatcaaga gccggccgca gcggtccatt attgtgctcg
      901 cggcctttgc gctggcggtg ctcgcggcgg ttctcctggc ggcggcggtg gactatgtgg
      961 accgcatgcg cacggccagc ccggagaatt accggcgggt ccagatgttc gcgcaggcgt
     1021 ggttcggctg gctgcccggg ctgaagcggc ggccggcgga ataagcgcgc gggcggggcc
     1081 gccgacggtg cgggcgcgcg gtctttcgaa cacgaaaaat tgaggataca cggccatgac
     1141 aagttcggaa atctcgccgg aggcgaggat tgaccccacc gcgcacatcg gccggttctg
     1201 cgtcatcggg gcgcaggcga cggtcggcgc gggctgcgtg atcggccacc acgtcgtcat
     1261 ccacgaggat acggccgtcg gcgacaacgt gcggatcgac gacggcgcgg tgctggggaa
     1321 gcgtcccatg cgcgcggcca actcggccag ggtcgcggcc gacggcgccg ccggctcccc
//
LOCUS       DAOOKS010000583         1380 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_126006_length_1380_cov_2.578868, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000583 DAOOKS010000000
VERSION     DAOOKS010000583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126006_length_1380_cov_2.578868"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /gene="hemB"
                     /locus_tag="PK112_08710"
     CDS             <1..598
                     /gene="hemB"
                     /locus_tag="PK112_08710"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HPC12163.1"
                     /translation="HGHCGVVEKGIVKNDESVEILAAQAVSHAAAGADMVAPSDMMDG
                     RVGRIRNALDAAGHAMIPIMAYSAKYASAFYGPFREAAGSTPQFGDRRGYQMDSANGR
                     EAMREIELDLAEGADIIMVKPALAYLDVIARARAAFPAPLAAYNVSGEYSMVKLAAER
                     GLADERTPTLEVLTSIKRAGADIIISYHAPDAAEWLGA"
     gene            complement(771..1223)
                     /locus_tag="PK112_08715"
     CDS             complement(771..1223)
                     /locus_tag="PK112_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HPC12164.1"
                     /translation="MRLKTAREILDFAIQKEQEAADFYNDLAERVDRPGMKSVFKGFA
                     KEELGHKAKLESVKAGNRMLAAEKKILDLKIGDHLVEVDLRPDLSYQEALIIAMKAEK
                     AAFKLYHDLAGTTDDPALREMLLGLAQEEAKHKLRFEIEYDDQILIEN"
BASE COUNT          208 a          449 c          468 g          255 t
ORIGIN      
        1 ccacgggcac tgcggagtgg tcgagaaggg aattgtcaag aacgacgaat cggtggaaat
       61 tctcgcggcg caggcggtga gccacgcggc cgcaggcgcc gacatggtgg cgccgagcga
      121 catgatggac ggccgggtgg ggcggatccg caacgcgctg gatgcggccg gccacgccat
      181 gatccccatc atggcctact ccgcgaaata cgcctcggcc ttctatggcc cgttccgcga
      241 agcggccggt tccacgccgc agttcggcga ccggcgcggc taccagatgg actccgccaa
      301 cggccgggag gcgatgcggg agatcgagct cgatctcgcg gagggcgccg acatcatcat
      361 ggtcaagccg gcgctggcgt atctggacgt catcgcccga gcgcgggcgg ccttccccgc
      421 gccgcttgcg gcctacaacg tgagcgggga gtacagcatg gtcaagctcg cggccgaacg
      481 cggtctcgcc gatgagcgga cgccgacgct ggaggtgctc acctcgatca agcgggccgg
      541 ggccgacatc atcatttcat accacgcccc cgatgcggcc gagtggctgg gggcatgaac
      601 agcgccgctc ccgcccggcc cacgccggcg gtgcgaccga cttgcgtcgg cctgctccgt
      661 gcctggcggc ggcattgacc cgatcgcatc ccgttacggt ttgacggatc ctaacgccgg
      721 tgttgccgtg cccggcgcgg tccgttttcg aaagcgctcg tgctcccagc tcagttctcg
      781 atcaggatct gatcgtcgta ctcgatctcg aaacggagct tgtgcttcgc ctcctcctgg
      841 gccaggccga gcaacatctc ccgcagcgcg ggatcatcgg tcgtgccggc gaggtcgtgg
      901 tagagcttga aggcggcttt ctcggccttc atcgcaatga tgagcgcctc ctggtaactc
      961 aggtcagggc gcaggtcgac ctcgaccagg tggtcgccga tcttgaggtc gaggatcttc
     1021 ttctccgccg cgagcatgcg gttgccggct ttgaccgatt cgagcttcgc cttgtggccc
     1081 agttcctcct tggcgaagcc tttgaagacg ctcttcatgc cggggcggtc gacccgctcg
     1141 gccagatcgt tgtagaaatc cgcggcctcc tgctccttct ggatggcgaa atcgaggatt
     1201 tctcgtgcgg tcttgagtct catgcatgtc ctcctgccgg tcgtcccgag cctggctggc
     1261 tcgttgattt tcgccggcag tatagtacag gggcgggccg gaggcaatgg ctagttcgcc
     1321 cgggcgggcc ccgggggcgg aggccgtcgc gagatactct gctcacccgg cggatccggg
//
LOCUS       DAOOKS010000584         1379 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_126163_length_1379_cov_2.413142, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000584 DAOOKS010000000
VERSION     DAOOKS010000584.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126163_length_1379_cov_2.413142"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1334
                     /gene="gltA"
                     /locus_tag="PK112_08720"
     CDS             <1..1334
                     /gene="gltA"
                     /locus_tag="PK112_08720"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012173746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HPC12165.1"
                     /translation="NEVPFGLSDEQAVIEAERCLQCKKPTCIEGCPVRINIPAFIKAI
                     AEKDFLRAIETIKQTNSLPAVCGRVCPQETQCEERCVLKKKFEPVAIGYLERFAADYE
                     RERERLTGVTPGADSGKKVAIIGSGPAGLTAAGELRKYGHDVTVFEALHSPGGVLVYG
                     IPEFRLPKDIVHYEIEALKKLGVKVVLNRVIGMSETIDDLMRQGFDAVFIGSGAGLPL
                     FLNVPGENLIGVYSANEYLTRANLMKAYKFPEYDTPIIKGRNIGVFGGGNVAMDSART
                     AKRLGAENVYIIYRRTEAELPARAEEVHHAKEEGIIFNLLSNPTQFLGDDKGRLRAVE
                     CLRMELGEPDKSGRPRPVPVKGSEFITDIDLAIIAIGNSPNPIIQKTTPGLEQSKWGT
                     IIVNEETMRTSKRGVFAGGDIVSGAATVILAMGAGRKAAQSMHEFLTTGQW"
BASE COUNT          292 a          448 c          414 g          225 t
ORIGIN      
        1 tcaacgaggt cccctttggg ctgagcgacg agcaggcggt catcgaggct gagcgctgcc
       61 tccagtgcaa gaaacccaca tgcatcgagg gatgccctgt ccgcatcaac atccccgcct
      121 tcatcaaggc aatcgccgag aaagatttcc tccgggcgat tgagaccatc aagcagacga
      181 actcgctacc ggccgtgtgc ggccgcgtct gcccgcagga gacccagtgc gaggaacgct
      241 gtgtgctcaa gaaaaaattc gaaccggttg ccataggcta cctcgagcgc ttcgccgccg
      301 actacgaacg ggagcgcgaa cgcctcaccg gcgtcacgcc cggcgccgac tccgggaaga
      361 aagtcgcgat catcggttcg ggtccggccg gcctcaccgc ggccggcgaa ctccgcaagt
      421 acgggcacga tgtcaccgtc ttcgaggcgc tccacagccc cggcggcgtg ctcgtctacg
      481 gcatcccgga gttccgtctc cccaaggaca ttgtccacta cgagatcgag gcgctcaaaa
      541 agctcggcgt gaaggtggtc ctcaaccgcg tcatcggcat gtcggagacc atcgacgacc
      601 tgatgcgcca gggctttgac gccgtgttca tcggctccgg cgcgggactc ccgctcttcc
      661 tgaacgtgcc gggagagaat ctgatcggcg tctactccgc caacgagtac ctcacgcgcg
      721 cgaacctcat gaaggcgtac aaattccccg agtacgacac tcccatcatc aagggcagga
      781 acataggcgt cttcggaggc gggaacgttg caatggactc ggcccgcacg gcgaagcgcc
      841 ttggcgcgga gaacgtctac atcatctacc gtcgcactga ggcggaactt cccgcgaggg
      901 cggaggaggt tcatcacgcg aaagaggaag gaataatatt caacctgctc tcaaatccca
      961 cgcagttttt aggggatgat aaaggccgcc tccgcgcggt ggagtgcctg cgcatggagc
     1021 tcggcgagcc cgacaaatcg ggccgcccgc gcccggtgcc ggtaaagggc tccgagttca
     1081 ttaccgatat cgacctggcg atcatcgcaa taggcaactc gcccaacccg atcatccaga
     1141 agaccacgcc gggactggag cagtcgaagt ggggcaccat catcgtgaac gaggagacca
     1201 tgcgcaccag caagcgcggc gtcttcgccg gcggcgacat cgtctccggc gccgccacgg
     1261 tgatactcgc catgggcgcc ggtcgcaagg ctgcccagtc catgcacgag ttcctcacga
     1321 caggccagtg gtgatcaaaa tcgggaaggc gacgatatat atcgtcgcct tcctgcttg
//
LOCUS       DAOOKS010000585         1376 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_126553_length_1376_cov_2.641938, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000585 DAOOKS010000000
VERSION     DAOOKS010000585.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1376
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126553_length_1376_cov_2.641938"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="PK112_08725"
     CDS             <1..196
                     /locus_tag="PK112_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015332279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HPC12166.1"
                     /translation="PQRWIKEVLDQDELILITRKGVTCRTAVKGISVIGRNTQGVRLM
                     QLRDDDTVIDVAHVVTDESQ"
     gene            291..515
                     /locus_tag="PK112_08730"
     CDS             291..515
                     /locus_tag="PK112_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HPC12167.1"
                     /translation="MRRLVIAVIGFTVLALGVALLVLPGPAFLVIPAGLGILSLEFVW
                     ARNLLRRLRRKLTGQPPADPAPPRSTGLGG"
     gene            complement(544..>1376)
                     /locus_tag="PK112_08735"
     CDS             complement(544..>1376)
                     /locus_tag="PK112_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HPC12168.1"
                     /translation="LAFMAFVYYRYETVSPGRGTPAALEFLTGYLIEKSLSIDNVFVF
                     LLIFSYFNVAPIYQHRVLFWGILGALVFRAFFIALGALLIAKFHAIIYLFGAFLVFTG
                     IKMAWAKDKQFHPERNPVLRLFRRFVGVTKDYRGKRFFVRAAGRRLATPLFVVLLLVE
                     SSDIIFAVDSIPAIFAVTKDPFLVFTANVFAILGLRSLYFALAGVMRLFHHLHYGLSV
                     ILVFVGIKMILSDIYKVPIGISLGAVGLIIAVSIAASLVWPKRAVAPGDPPALPEIPA
                     "
BASE COUNT          284 a          454 c          437 g          201 t
ORIGIN      
        1 gccgcagcgc tggatcaagg aggtcctcga ccaggacgaa ctgatcctca tcacccgcaa
       61 gggcgtgacg tgccgcaccg cggtcaaggg gatcagcgtc atcggccgca acacccaggg
      121 cgtgcggctc atgcagctgc gcgatgacga caccgtgatc gacgtcgccc acgtggtcac
      181 cgacgaatcg cagtgagcgg gcgccggccg ccccgcctct caccgacccg ggactgctcc
      241 cggcgtaccg gtgaccgggg gaagcgatga ttgccggaac gataagacaa gtgcgaaggc
      301 tggtcatcgc cgtgatcggt ttcacggtgc tcgcgctcgg cgtagctctc ctggtgctgc
      361 ccggcccggc gtttctcgtc attccggccg gcctcggcat cctctccctt gagttcgtgt
      421 gggcgcgcaa cctgctgcgc cggctgcgcc gcaagctcac cgggcagccc ccggccgacc
      481 ctgcgccccc gcgctccacg ggcctcgggg gctgacctcc cggaccggcc ggaactttcc
      541 gacttacgcg ggaatctcgg gcagggccgg cggatcgccg ggcgcgaccg cgcgtttcgg
      601 ccaaaccagc gaagcggcga tcgacaccgc gatgatcagt cccaccgccc ccagagagat
      661 gccgatcgga accttgtaga tatccgagag gatcattttg atcccgacaa agacgaggat
      721 gaccgacagg ccgtagtgga ggtggtggaa cagccgcatc acgcccgcca gcgcgaagta
      781 gagcgagcgc aatccgagaa tcgcgaacac gttggccgtg aacaccaaga acggatcctt
      841 cgtcaccgcg aaaatcgccg gtatcgaatc gaccgcgaat atgatgtcgc tggactccac
      901 gaggaggagc acaacgaaca aaggcgtcgc cagccggcgg cccgccgctc tgacgaaaaa
      961 gcgcttcccc cggtaatcct ttgtgacccc gacgaacctc cgaaagagtc gcagcaccgg
     1021 gttccgctcc gggtggaact gcttgtcttt cgcccaggcc atcttgatcc ccgtgaacac
     1081 caggaaagcg ccgaagaggt agatgatggc gtggaatttc gcgatcagga gggcgcccag
     1141 ggcgatgaaa aacgcccgga acacgagcgc cccgagaatc ccccagaaca acacccggtg
     1201 ctggtatatc ggagccacgt tgaagtacga gaagatgagg aggaagacga agacgttgtc
     1261 gatgctcagc gacttctcga tgaggtagcc ggtgagaaac tccagcgccg ccggagtgcc
     1321 ccgcccgggc gagaccgtct cgtaccggta gtacacgaac gccatgaacg cgagcg
//
LOCUS       DAOOKS010000586         1375 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_126655_length_1375_cov_3.209091, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000586 DAOOKS010000000
VERSION     DAOOKS010000586.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1375
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126655_length_1375_cov_3.209091"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            197..991
                     /locus_tag="PK112_08740"
     CDS             197..991
                     /locus_tag="PK112_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12169.1"
                     /translation="MPWNSPANQRRLRRLFDEGFSAMDIAEPLASFDDDRSAAAVRAF
                     LEQRDLMVAGVRRDGFVCGFADRRELGRGRLGAYLRPFPPECILSEADSLRKVILAMR
                     GGGPCFVSVLGEVGAVITLADLEKPPVRMFLFGMITIFEMIMTWGIRTYFPGDSWRAH
                     IPPGRLAKAEQLWQERQRRRSTADLLDCLQFSDRAQLLLRIPRVTDLLAQGGIPSRRA
                     ALTAIGELETLRNNIAHSQEIIPESWSRIVVFSTRLELLLGEAFRS"
     gene            1015..>1375
                     /locus_tag="PK112_08745"
     CDS             1015..>1375
                     /locus_tag="PK112_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12170.1"
                     /translation="MRSCKLAGAALLLAVLAWPLAAQDAPPVDGGWPRTFTTASDGEM
                     TLFAPQVAAWENQERLVAFCAVSYLPAGATERRLGTIKIHAATEVSLEERLVRITNLQ
                     IVESNFPGLPKDQVRGLL"
BASE COUNT          216 a          460 c          447 g          252 t
ORIGIN      
        1 gctgttgaca ttcgggaggg cgcatagtat cgtatcttct gacggcaggt agtcggactc
       61 gtgccgccgc ccgcccgcag atcgccggcg gcggccctga cgtgaggttc ccttcattgt
      121 ccaaaaccga acagcaggac aggatccgct ctcaccggcg gacggttccc tgttgccgca
      181 ccggggagtc tgtgctatgc cctggaacag cccggccaat caaaggcgcc tgaggcgcct
      241 gtttgacgag gggttctcgg cgatggacat cgccgagccg ctcgcgtcgt tcgacgacga
      301 ccgttcggcg gcggcggtcc gggctttcct ggagcagagg gatctcatgg tcgcgggtgt
      361 gcgccgcgac ggcttcgtgt gcggttttgc cgacaggcga gaactcggcc gcggccgcct
      421 gggcgcttac cttcgcccgt tccctcccga gtgtatcctc tccgaggccg attcccttcg
      481 caaagtgata ctggcgatgc gggggggagg cccctgtttc gtcagcgtac tgggtgaagt
      541 cggcgccgtc atcacgctgg ccgatctcga gaagcccccg gtccggatgt tcctcttcgg
      601 gatgatcact attttcgaga tgatcatgac gtggggcatt cggacatatt tccccggcga
      661 ttcctggcgc gcccacatcc cgcccggacg gctggccaaa gccgagcaac tgtggcagga
      721 gagacagcgc cgccggagca ccgccgatct gctggattgc ctccagttct ccgatcgggc
      781 gcaactgctg ctgaggatcc cgcgcgtgac ggacctcctg gcccagggag ggattccatc
      841 ccggcgggcg gcgctcaccg cgattggcga actggaaacg ctccgcaaca atatcgccca
      901 ctcccaggag atcatccccg agagctggtc ccgcatcgtc gtcttttcca cgcgcctgga
      961 actgctcctc ggcgaggctt tccggagtta gcgccgagag gaggaatact gactgtgaga
     1021 agctgcaaac tggcgggtgc ggcgcttctg cttgccgtcc tcgcctggcc gctggcggct
     1081 caggatgcgc caccggtcga cgggggctgg ccccggacct tcactaccgc aagcgacggc
     1141 gaaatgacgc tcttcgcccc gcaggtcgcc gcctgggaga atcaggagcg gctcgtcgca
     1201 ttctgcgccg tgtcctacct cccggccggg gcgaccgagc gccgcctggg cacgatcaag
     1261 atccatgcgg ccaccgaggt ctcgctcgag gagcggctcg ttcgcataac caacctccag
     1321 atcgtcgagt cgaacttccc cgggctcccc aaagaccagg tgcgcgggct gctcg
//
LOCUS       DAOOKS010000587         1374 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_126841_length_1374_cov_2.517817, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000587 DAOOKS010000000
VERSION     DAOOKS010000587.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126841_length_1374_cov_2.517817"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="PK112_08750"
     CDS             complement(<1..345)
                     /locus_tag="PK112_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12171.1"
                     /translation="MTRILAVLAALVLLATAAGFGDDDESRDWNTAPASEIHERLAAA
                     KAGDLQRRTALKQLLGGSEATTQTNYDVTFYDVKLRINDTTEIVWGRVTFVATAAEGN
                     VGKELIAAAAGEI"
     gene            complement(588..>1374)
                     /locus_tag="PK112_08755"
     CDS             complement(588..>1374)
                     /locus_tag="PK112_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12172.1"
                     /translation="MGIRNLPPVGGFIVDNAGGDDPMEFVLGWESELFDISTLSNVAP
                     ATVSVQANFLNLPIGVYYDTIVVTARKSTNPADTVIVKYSVIPPTQQPKIWVSNSSFV
                     VPAQENSGPLPDIGMAIHNEFGGCMPWYIVENVPWLFPSDTSGINPASLIFGVNSDGF
                     LFGEYPDSIKLYAPSASNSPGTIRVRLKVWRFHGDCDYNSVINVLDLTYMISFLFRGG
                     PQPQPTQLVGDLNCDHLVNIADLTYFIEYLFRNGPIPCWNPYK"
BASE COUNT          271 a          448 c          395 g          260 t
ORIGIN      
        1 tatctctcca gccgcggcgg cgatcagctc cttgccgaca ttcccctcgg ccgccgtcgc
       61 cacgaacgtc acccgccccc acacgatctc ggtggtgtcg ttgatccgga gcttcacatc
      121 gtagaatgtg acgtcgtagt tcgtctgcgt ggtcgcttcg gacccgccga ggagctgttt
      181 cagggcggtg cgccgctgca ggtcccccgc cttggccgcc gccagacgct cgtgaatttc
      241 cgacgccggg gcggtgttcc agtcccggct ctcatcgtca tccccgaaac cggcggccgt
      301 cgcaagcagc acaagcgccg ccagaaccgc caggatgcgt gtcaccgatg ctgagctcaa
      361 tggtgcctcc aaactctcta tggaccgatc acttgttatt catcgtcggc ggaccaaggt
      421 acaactgtat gtacggggag cgtcgatcgt tccgcaaaat aaccgatccg ccccgtaagt
      481 caaggccaat tcggctgtta gccgaggcgc acattttgcc ccgcgcccat agaaaaacgc
      541 cgcggctttc cgccgcggcg cctctccagc cggccttcac cgggccgtca cttgtacggg
      601 ttccaacacg gtataggacc gttgcggaac agatattcga tgaaatacgt gaggtcggcg
      661 atattcacca ggtggtcgca gttcagatcc ccgaccaact gcgtcggctg cggctgggga
      721 ccgccccgga acaggaacga aatcatgtag gtgaggtcaa gaacgttgat caccgagttg
      781 tagtcgcagt ccccatgaaa acgccacact ttcaggcgca cccggattgt gcccgggctg
      841 ttggaggccg agggagcgta cagcttgatc gaatccggat actctccgaa caggaacccg
      901 tccgaattca ccccgaagat cagcgacgcc gggttgatcc cggaggtgtc ggaggggaac
      961 agccagggga cgttttccac gatgtaccac ggcatgcagc cgccgaattc attgtgaatc
     1021 gccatcccga tatccggcag cggcccgctg ttctcctgcg ccggcacgac aaacgagctg
     1081 ttcgaaaccc agatcttggg ctgctgcgtc ggcggtatga ccgagtactt cacgatcacc
     1141 gtgtccgccg gattggtcga tttccgcgcc gtcacgacaa tggtgtcgta gtacaccccg
     1201 atcggcagat tcaggaaatt cgcctggacc gagacggtcg cgggcgccac gttgctcagg
     1261 gtcgagatat cgaacaactc agactcccaa ccgagcacaa actccatcgg atcgtccccg
     1321 ccggcattgt cgacgatgaa cccgccgacc ggcggcaggt tgcgtatccc catc
//
LOCUS       DAOOKS010000588         1370 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_127376_length_1370_cov_3.591635, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000588 DAOOKS010000000
VERSION     DAOOKS010000588.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127376_length_1370_cov_3.591635"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="PK112_08760"
     CDS             <1..571
                     /locus_tag="PK112_08760"
                     /inference="COORDINATES: protein motif:HMM:NF017509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12173.1"
                     /translation="ATVRYRTTVKEVREEIQPAFDDYLAKRTGHAQTALELRMKIRED
                     LKAQKERELRRYHRNEIVQQVIERNKVSVPAGLVKEYLDAMVKDLRRQDSTADEAELR
                     GAYHGMATAALQWNLLSDHIARQENIQVTREDTDKVIKRFAENYEITEAEAREGLRRS
                     GKYDNITDTLREEKVLDFLISKAEVVKKE"
     gene            620..1237
                     /gene="clpP"
                     /locus_tag="PK112_08765"
     CDS             620..1237
                     /gene="clpP"
                     /locus_tag="PK112_08765"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HPC12174.1"
                     /translation="MDEKIQAQYLVPMVVEQTGRGERAYDIFSRLLKDRIIFIGTPID
                     DNVANLVIAQMLFLEAEDPDKDIFVYLNSPGGSVTAGLAIYDTMQFIKPDVVTTCMGI
                     CASMGAFLLAAGTHGKRAALPNSRVMIHQPLAGTQGQISDIVIMTKEFTRTKERLNDL
                     LAQHTGQPLERIEKDTDRNFFMSASEAKDYGLIDKVYDFKRSERK"
BASE COUNT          319 a          380 c          457 g          214 t
ORIGIN      
        1 agcgaccgtg cgctaccgga caacggtgaa agaggtgcgc gaggagattc agccggcgtt
       61 cgacgactac ctggccaaga ggacgggaca cgcccagacg gctctggagt tgcggatgaa
      121 gatccgggag gatctgaagg cccagaagga gcgggagttg cgccggtacc accggaacga
      181 aatcgtccag caggtgattg agcggaacaa ggtctcggtc cccgccggac tggtgaagga
      241 gtacctcgac gcgatggtca aggacctccg gcgccaggac agcacggcgg acgaagcgga
      301 actgcgcggc gcgtaccacg ggatggcgac ggcggcgctg cagtggaacc tgctctctga
      361 ccatattgcc cggcaggaaa acatacaggt tacgcgggaa gataccgaca aagtaataaa
      421 gcggttcgcg gagaactatg agatcaccga agcggaggcg cgcgaggggc tccggcggtc
      481 gggcaagtac gacaacatca cggacacctt gcgcgaggaa aaagtgcttg atttcctcat
      541 ttccaaggcc gaggttgtga agaaggaata gccgcgacga cgaaaacgga ctgaggtcca
      601 aggagttatg caggagatta tggacgaaaa gatacaggcg caatacctgg tgccgatggt
      661 ggtcgagcag accggccgcg gggagcgggc gtacgatatc ttctcgcgtt tgctgaagga
      721 ccgcatcatc ttcatcggga cgccgatcga cgacaacgtg gccaacctgg tcatcgccca
      781 gatgctcttc ctcgaggcgg aggatccgga caaggacatc tttgtctatc tgaactcgcc
      841 gggcgggtcg gtgacggcgg ggctggccat ctacgatacg atgcagttca tcaagcccga
      901 cgtggtgacg acgtgcatgg ggatttgcgc ctcgatgggg gcgttcctgc tggccgccgg
      961 cacccacggc aagcgggcgg cgctgcccaa cagccgggtg atgatccacc agccgctcgc
     1021 cggcacccag gggcagatct cggacatcgt gatcatgacc aaagagttca cccggaccaa
     1081 agagcgcctc aatgatcttt tggcccagca taccgggcag ccgctcgaac ggatcgagaa
     1141 agataccgac cggaacttct tcatgtcggc ctcggaggcc aaggactacg gcctgatcga
     1201 caaggtctac gatttcaagc ggtcggagcg caagtaggag ctagaccggg cgagggaatc
     1261 gccggtcggg aaacggatat aggatatgtc agcggactcc gagaaacccc gtccgccgca
     1321 gcgctgctca ttttgcggca agccggccag ccaggcgaaa cggctctttt
//
LOCUS       DAOOKS010000589         1370 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_127463_length_1370_cov_2.062357, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000589 DAOOKS010000000
VERSION     DAOOKS010000589.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127463_length_1370_cov_2.062357"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            49..615
                     /locus_tag="PK112_08770"
     CDS             49..615
                     /locus_tag="PK112_08770"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HPC12175.1"
                     /translation="MPDSFFRWHHLCRAQYLEARSLLSGYLLSSQGDRVGMAHSVEGR
                     FPFLDHRVVEFAATIPPWHKLFGLKEKFVLKRAMARELPEEITARVKQPYMAPDSNSF
                     VQPDSPGYIADILAESEIARTGLFESAAVQQLREKATRLKHAHLSFKDNMSMIGILST
                     QLLIHAFIDRFQPAPEPGRSAFSVWHEA"
     gene            complement(760..>1370)
                     /locus_tag="PK112_08775"
     CDS             complement(760..>1370)
                     /locus_tag="PK112_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12176.1"
                     /translation="LITDPTGEARQRLERAAQEPTIVFRRGARPAMEGETASAVPAAG
                     SGALPGPEPLPDLPDDAPAAPTARPYPSPVPPIAVRESLHDRFADIPGFEHAYSMTLD
                     GKFKSAAGEEQFRKGFKHVHKTLRDVIELFPEKGGARRRREEGVCEVERNRLYLVSTG
                     EELVFITINSIRTDLNYEKIMKNLLFPDELQVLLQQRGADGK"
BASE COUNT          223 a          479 c          411 g          257 t
ORIGIN      
        1 cgccggattc tcgatggtca cagctcgctg taggctttct gcgtcgacct gccggactcg
       61 tttttccgct ggcatcacct ctgccgggcg cagtatctcg aagcacggtc gctcttatcg
      121 ggctacctgc tctcgtcgca gggggaccgc gtcggcatgg cccactcggt cgagggccgc
      181 ttccccttcc tcgatcaccg ggttgtcgaa tttgcggcga ccatcccgcc ctggcacaag
      241 ctcttcggcc tgaaagagaa attcgtgctg aaacgggcga tggcgcgcga gttgccggag
      301 gagatcacag cgcgcgtgaa acagccgtac atggcccccg actccaacag ctttgtccag
      361 cccgactcgc cgggctacat cgccgacata ctcgccgagt ccgagatcgc gcgcaccggt
      421 ttgttcgaat cggccgcggt ccagcagttg cgcgagaagg cgacccggct caagcacgcc
      481 cacttgtcat tcaaagacaa catgtcgatg atcggcattc tctcgaccca gctgctcatc
      541 cacgctttca tcgaccggtt ccagcccgcc cccgaacccg gccgcagcgc cttttccgtt
      601 tggcacgaag cttaggccgg gcaaacacca tccgtatcga cggtcgatcg agaatgtcag
      661 cggtgagatt cgcccccgtg agaactgccg ctccggactg agtcatgaga cgcgcgccgg
      721 caccgtatca ggaataccct gcgcccggcc tgccccccct tacttcccgt ccgcccccct
      781 ctgctgcaac agcacctgca gctcgtccgg aaagagcagg ttcttcatga tcttctcgta
      841 gttcagatcg gtccggatgc tgttgatcgt aatgaagacc agttcctccc ccgttgacac
      901 gaggtacagg cgattccgct cgacctcgca cacgccctct tcccgccgcc gtcgcgcccc
      961 gcccttctcg gggaacagtt cgatcacgtc gcgaagcgtt ttgtgcacgt gtttgaatcc
     1021 cttgcggaac tgctcttcgc cggccgccga cttgaacttc ccgtccagcg tcatgctgta
     1081 ggcgtgctcg aaaccgggga tgtcggcgaa gcggtcgtgg agcgactccc tgaccgcgat
     1141 cggcggcacc ggcgaggggt acggccgagc ggtgggcgcc gcgggcgcgt catcgggaag
     1201 atccggaagc ggctccggac cgggcaacgc acccgatccg gcggccggaa ctgcggacgc
     1261 cgtctcgccc tccatggccg gccgggcgcc gcggcgaaac acgatggtcg gctcctgagc
     1321 ggctcgttcg agccgttgcc gcgcctcgcc ggtgggatcg gtgatgagcg
//
LOCUS       DAOOKS010000590         1365 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_128155_length_1365_cov_3.160305, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000590 DAOOKS010000000
VERSION     DAOOKS010000590.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128155_length_1365_cov_3.160305"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..122)
                     /locus_tag="PK112_08780"
     tRNA            complement(48..122)
                     /locus_tag="PK112_08780"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(88..90),aa:Asn,seq:gtt)
     gene            907..>1365
                     /locus_tag="PK112_08785"
     CDS             907..>1365
                     /locus_tag="PK112_08785"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HPC12177.1"
                     /translation="MKRFWLAAIVALAALGLLAATGLAGKDTDERAWLGVYTQSVDYD
                     LADAFDLTVSYGAVINEVVEDSPADQAGLKNGDVIISLNGEKITDSDDLIDVLHEASP
                     GDRLELVVIRDGKEQSVSVVAGARSEEDDNLYGTFRSYFDGGNHSAYTLSG"
BASE COUNT          242 a          488 c          387 g          248 t
ORIGIN      
        1 ggggtcttgc gttaaacaag aaccgctgaa atcagcggat tgaattttgg ctccggtggc
       61 tggactcgaa ccagcggccc gctgattaac agtcagctgc tctaccactg agctacaccg
      121 gagtaatcaa aaaaccgcgc ttcgcccgca ggcgaagctc accattaata ccgtttttcc
      181 cgccaaagtc aactcctttt tcccgcaccc ctcacccgat ccgctctcag tcgatctgcc
      241 ctcagccgat ccgctctcag ccgatccgcc ctcagccgat ccgctcttag ccgacccgct
      301 cttactcgat ccgcttccgg ccgacccgcc cacacccgat cggtcctccc ggcccccatc
      361 gtccgggcgg cccgtcgtcg gaggcatgga ccacgcgcac ctcggggatg tccctgctcc
      421 cgccgaatcg cccgggatcg ccacagcgat cactacggat tcggcgtagt cattgcgact
      481 gccaaccttc tccccacccg cgcgagtccg caaggcagcc atagattcgc ggaccgcgcg
      541 ccatcggttc cccggcagtg tgcccctgca catgagcgac ccggtgcaat ccacgcggcc
      601 ggtcaatcca gacggtcggg cgtagccggc cggatgtcgg gggcgtgacg tacaacttgt
      661 tgccccgcat atgagtagcc ccgccatgag actctgccgg gacggggcgg ggcggggaga
      721 ggagaggacg ggagaggctc cgcgacccgg ctcgcaccgt ctgcaccggc acccccgggc
      781 cgacggcata tctcctgcgc tccggcgccc cgccctctcg gtctctcccc cgtgaaacct
      841 cgccggacgt cccgcgtata tcgctttagc aacctgaaca gtctgaccca tggaggatat
      901 agcgctatga aacgattctg gctcgcggcg atcgtggctc tggcggccct cggcctgctc
      961 gccgccaccg gtctcgccgg aaaagataca gatgagcgcg cctggctggg ggtctatacg
     1021 cagtcggtcg actacgatct ggccgacgct tttgatctca ccgtgtccta cggcgccgtc
     1081 atcaacgagg tggtcgagga ttcccccgcc gaccaggccg gcctgaagaa cggggatgtc
     1141 attatctcgc tcaacggcga aaaaatcacc gactccgatg atttgatcga tgtcctccac
     1201 gaagcctcgc ccggcgatcg cctcgaactg gtcgtcattc gcgatggcaa agagcagagt
     1261 gtctccgttg tcgcgggcgc gcggtcggag gaggacgaca acctctacgg cactttccgc
     1321 tcctacttcg acggcggcaa ccattccgcg tacacgttgt cgggc
//
LOCUS       DAOOKS010000591         1365 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_128167_length_1365_cov_2.861832, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000591 DAOOKS010000000
VERSION     DAOOKS010000591.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128167_length_1365_cov_2.861832"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..885)
                     /locus_tag="PK112_08790"
     CDS             complement(<1..885)
                     /locus_tag="PK112_08790"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPC12178.1"
                     /translation="MAFLEVQGLARSYRGVRAVEDFSFAMERGEIYALVGPDGAGKTT
                     VIRAIANLITPDRGRILLDGTDIRDRFDRVKSRLGYMPQVFSLYPDLSVEENLIFYAG
                     VYGVTGRAYEERRDRMYDFSNLRPFAGRRAAALSGGMKQKLALSCALMHDPELLLLDE
                     PTTGVDPLSRRQFWDILLQLKRDGAAVLVTTPYMDEVARADRGSFVFGGRKLAEGSPR
                     ELTESFSGEVYFVGAAADLELVRRLNELPEVSAQRFGAGLHLYLPRGGRLEPHLPALA
                     ACGIRPEQIRKIRPDLEDA"
     gene            complement(885..>1365)
                     /locus_tag="PK112_08795"
     CDS             complement(885..>1365)
                     /locus_tag="PK112_08795"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HPC12179.1"
                     /translation="HTQAGIAVGSARSAIASAEAEIAKIDADIARLLDQWDDAFPVAP
                     IAGTVTEKYVDAGELLAPGRPIARIARLDTVWVKIYLPAREFAAVTIGAAATVDTEAG
                     ESSPGRVTWVADQAEFTPKNVQTKKARADLVYAVEITVANPAHKLKIGQPVFVTVGE"
BASE COUNT          227 a          451 c          480 g          207 t
ORIGIN      
        1 ggcgtcttcg aggtcgggac ggatcttccg gatctgctcc ggccgaatgc cgcacgccgc
       61 cagagcgggc aagtggggct cgaggcggcc gccccgcggc agatacaggt gcaggccggc
      121 gccgaagcgc tgggcgctca cctcaggcag ctcgttgagc cggcggacaa gctcgaggtc
      181 ggcggccgcg ccgacgaaat agacctcgcc cgaaaacgac tcggtcaact cgcgcggcga
      241 cccctcggcg agtttccgcc cgccgaagac gaaactcccg cggtcggcgc gggccacttc
      301 atccatatag ggggtggtga cgagcacggc ggccccgtcc cgcttgagct gcagaagaat
      361 gtcccagaac tggcggcggg agagcgggtc gacgccggtg gtgggctcat cgagcagcaa
      421 cagctcgggg tcgtgcatga gggcgcagga gagcgccagt ttctgcttca tgccgccgga
      481 gagggccgcc gcccggcggc cggcgaaggg gcggaggttc gaaaaatcgt acatccggtc
      541 gcgccgctct tcatacgccc ggccggtcac gccgtagacc cccgcgtaga aaatgaggtt
      601 ctcctccacg ctcaggtcgg ggtagagcga aaatacctgg ggcatgtacc cgaggcggga
      661 tttgacccga tcgaaccggt cgcggatgtc ggtcccgtca agcaggatgc ggccgcggtc
      721 gggcgtgatg agattggcga tggcgcggat gacggtcgtc ttgccggcgc cgtccggccc
      781 gacaagggcg tagatctcgc cgcgctccat ggcgaaggag aagtcctcga ccgcccgcac
      841 gccgcggtac gaccgggcca gaccctggac ctcgaggaaa gccatcactc ccccaccgtc
      901 acaaacaccg gctggccgat tttcagtttg tgggccgggt tggcgaccgt gatctcaacg
      961 gcgtacacca gatcggcccg ggcctttttg gtctgcacat tcttgggcgt gaactccgcc
     1021 tggtcggcca cccacgtgac ccggccggga gaggattccc ccgcttcggt atcgacggtg
     1081 gcggcggcgc cgatagtcac ggcggcgaac tcccgcgccg gcagatagat cttgacccac
     1141 acggtgtcga gccgggcgat gcgcgcgatc ggccgtccgg gggcgagcag ttcccccgca
     1201 tcgacatact tctccgtcac cgtcccggcg atcggggcga ccggaaaagc atcgtcccat
     1261 tggtccaaaa ggcgggcgat gtcggcgtcg atcttggcga tttccgcctc ggccgaggcg
     1321 atcgccgagc gggcgctgcc gaccgcaatt ccggcctggg tgtgg
//
LOCUS       DAOOKS010000592         1362 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_128623_length_1362_cov_2.368018, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000592 DAOOKS010000000
VERSION     DAOOKS010000592.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128623_length_1362_cov_2.368018"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(386..1078)
                     /locus_tag="PK112_08800"
     CDS             complement(386..1078)
                     /locus_tag="PK112_08800"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HPC12180.1"
                     /translation="MRKVFTVALVLAVAMMFAGAAAYADGSCAVKKTAESASAKSACT
                     AGKTAEAKMTSAENAGCSGHKTGETAMTAAGSCANAKFMAGCDPSFCTPEDCAAWGKM
                     CQAHGENAEVRMMSVKGMTCTGCENSIKAALEKAPGVLEVAKVDHKRGLAVVIVDKTM
                     AKNDDMTTLVTNKGYEAQIIPAVATTGTAAPAAKVSDGAKKSCAATCAKPCDKAKSTE
                     TKAGDKSTSTPH"
     gene            1123..>1362
                     /locus_tag="PK112_08805"
     CDS             1123..>1362
                     /locus_tag="PK112_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12181.1"
                     /translation="MVQYTFFYPASQARLPVCGYFWAMMCCIGVIGIGYPTYRYVGLT
                     PQTGGWFQKKAYYFSPLLPSSLSSIGYRKKNLDRTR"
BASE COUNT          247 a          382 c          439 g          294 t
ORIGIN      
        1 atcaagcggg ccctcgtagc cggagcgccg agcggcgtcg aggtcgaacc ggccgtcggg
       61 ggtgaggtac tcacgtatgc ttttgcctcg cggttgagtc ggcaagggat cttcggcaag
      121 gatcgaacct gcgaaaatca ggcagagggc gagagaaaca aatgtgtact tcatggaaac
      181 actccggcgt ctatgcggcc gggcggccgg cgatttgaac tccctacgtc aacgggaata
      241 agatgccaaa aatggagtat ttggtcaagg agaatgccga ggagcagggg cggaaaccgg
      301 cgcgcgcggg agcagaggcc acgtgaaaac ggcgggagcg atcgctcccg ccgttcgggt
      361 tcagtctata agcctgaggc cgtgatcagt gcggggtcga ggtcgacttg tcgcccgcct
      421 tggtctcggt cgacttggcc ttgtcgcacg gcttggcgca ggtcgcggcg cacgacttct
      481 tcgcgccgtc ggaaaccttc gccgccggag cggcggtgcc ggtcgtggcg acggccggga
      541 tgatctgagc ttcgtaaccc ttgttggtca cgagggtggt catgtcgtcg ttcttcgcca
      601 tggtcttgtc gacgatgacg acggcgaggc cgcgcttgtg gtcaaccttc gcgacttcga
      661 ggacacccgg cgccttctcg agggcggcct tgatggagtt ctcgcagccg gtgcaggtca
      721 tgcccttgac cgacatcatg cggacttcgg cgttctcgcc gtgggcctga cacatcttgc
      781 cccaggccgc gcagtcctcg ggagtgcaga acgacggatc gcagccggcc atgaacttgg
      841 cgttggcgca actgccggcc gcggtcatcg ccgtctcccc ggtcttgtgg ccggagcagc
      901 cggcattctc agccgaggtc atcttggctt cggcagtctt gcccgccgtg caggccgact
      961 tggccgaggc ggattccgcg gtcttcttga cggcacagga gccatcggca taagctgcgg
     1021 cgccggcaaa catcatggcg accgccagaa cgagggcgac ggtgaatact tttctcatgg
     1081 tgttactcct cttcacacgg gtacatcagc ggttcaggtt ttatggtaca gtatacgttt
     1141 ttttatccgg ccagtcaagc gcgattaccg gtttgtggat atttttgggc gatgatgtgt
     1201 tgtattggcg tcatcggcat cggctatccc acctatcggt atgtcggttt aacaccccag
     1261 accgggggat ggtttcagaa aaaagcgtac tatttcagtc ccttattgcc atcgtcttta
     1321 tcttcaatcg gttaccggaa gaaaaacctt gatcgcacaa gg
//
LOCUS       DAOOKS010000593         1361 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_128727_length_1361_cov_2.916539, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000593 DAOOKS010000000
VERSION     DAOOKS010000593.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128727_length_1361_cov_2.916539"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1361)
                     /locus_tag="PK112_08810"
     CDS             complement(<1..>1361)
                     /locus_tag="PK112_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12182.1"
                     /translation="VTAMHMSLLEGTIYDYAAWVYPTVSRGGFGLGVMRLGTGDIVRR
                     EEFIETGTFDYVQSQILLSYGRRFHPHFAAGASLKVVYQTLDVYSNWGVGADLGLLAR
                     PHEQVSVGMVLRDVIPAGLTLNQEEETAPLSVAGGMSVSGLRIRDDIAGLISFELEQI
                     EDRAVRVHTGGELLFAGAYAVRAGWDRDQLAFGAGFRRGRFQVDYTYKLMDYIPDSHR
                     FSISYDIGPSVAEQAEEQRLAELRKGTDLLEEERRRQFNLYKQKADEFRARFRLDSAL
                     TYYQRALAFDENNQEIIGAIAAMESTQRIQQSEQTDLLERQHELNRMMASYLEQARNF
                     AGKEYYPAALDMIQLIFEVDPNYAPARALKAGIEAAMATAVSETLETAREARRDGNTV
                     AAIEAYERVLYLDPNNVTAQRGKEALARSLDVGRHLNTGIDLFKAGRYGDARRQFELV
                     LSA"
BASE COUNT          224 a          475 c          422 g          240 t
ORIGIN      
        1 tggccgagag cacgagctcg aactgccgcc gcgcatcccc gtaccggccg gccttaaaca
       61 ggtcgatgcc ggtgttgagg tgccgcccga catcgagact ccgggccagc gcttcttttc
      121 cccgctgcgc cgtcacgttg ttggggtcga ggtagaggac gcgctcgtag gcctcgatcg
      181 cggcgaccgt gttgccgtcg cgccgcgcct cgcgggcggt ttcgagagtt tccgacaccg
      241 cagtcgccat cgccgcctca atccccgcct tgagcgcccg cgccggcgcg tagttcggat
      301 cgacctcgaa aatcagctgg atcatgtcca aggccgcggg gtagtactct ttccccgcga
      361 aattccgcgc ctgctcgaga taggacgcca tcatccggtt caactcgtgc tgccgctcga
      421 gcagatccgt ctgctcgctc tgctggatcc gctgcgtgct ctccatggcc gcgatcgccc
      481 cgataatctc ctgattgttc tcgtcgaacg ccagcgcccg ctggtagtag gtcagggcgg
      541 aatcaagccg gaagcgggcg cggaattcgt ccgccttctg cttgtacaga ttgaactgcc
      601 gccgccgctc ttcctcgagc agatcggtgc ctttgcgtag ttccgccagg cgctgttcct
      661 cagcctgctc ggcgaccgag ggcccgatgt cgtaggagat ggagaaccgg tgcgagtcgg
      721 ggatgtagtc catcagcttg taggtgtagt cgacctggaa ccgcccgcgg cggaagccgg
      781 caccgaaagc caactggtcg cggtcccagc cggcccgcac ggcataagct ccggcgaaca
      841 ggagctcgcc gccggtgtgg acccgcaccg cccggtcctc gatctgctcc agctcgaacg
      901 agatcagccc cgcgatgtcg tcgcgaatcc gcagcccgct gaccgacatg cctccggcca
      961 ccgacagggg cgcggtttcc tcttcctggt tgagagtgag gccggccgga atcacgtccc
     1021 gcagcaccat ccccaccgaa acctgctcgt gcggccgggc gagcagcccg agatccgccc
     1081 ccactcccca gttcgagtag acgtcgagcg tctggtacac gactttgagc gaggccccgg
     1141 cggcgaagtg ggggtggaac cgccgcccgt aggacagcaa aatttgcgat tgcacatagt
     1201 caaacgtgcc ggtttcaatg aactcctcgc ggcgcacgat atcgcccgtt cccaggcgca
     1261 tgacgccgag cccgaacccc ccgcgcgaca cggtcgggta cacccaggcc gcgtagtcgt
     1321 agatcgtccc ctcgagcagc gacatgtgca tggccgtgac t
//
LOCUS       DAOOKS010000594         1359 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_129005_length_1359_cov_3.305215, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000594 DAOOKS010000000
VERSION     DAOOKS010000594.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129005_length_1359_cov_3.305215"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            296..>1359
                     /locus_tag="PK112_08815"
     CDS             296..>1359
                     /locus_tag="PK112_08815"
                     /inference="COORDINATES: protein motif:HMM:NF020028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase"
                     /protein_id="HPC12183.1"
                     /translation="MSHTATHIPAAPTWDLDSIFPGGSASAEYKAFREKVKKDLAGAA
                     TAVDRLPAAITPENRPQWAAAISTLQSILADLELLLAFASCLKAQNVSDTLAHAADAE
                     GEEDVAQWKKLMTRIEAMSLQQSDAAWQLLTAAPELRGTEFFLNEVRETARRKMPVEK
                     EELALDLAVNGYHTWNQLYDKMAGDLREPFEEGGQTKLLSMGQIATKMSSPDREVRRR
                     AFEAMTNGWKRRQDLAAMALNAQAGFRLSLYKYRGWDLMYEPLRNARLQEATLDTMWR
                     VIAEEKKRLRAYVDAKKRLLGIDRFRWYDEFAPAGRSDRQYSFAEAGDFVVAQVRSFS
                     DDMADFYRRAIDSRWVEAEDR"
BASE COUNT          244 a          431 c          464 g          220 t
ORIGIN      
        1 ccggcggcgg cgaatgttga cggcgccggc gcgccagggg ccgacattac ggtcgacgac
       61 ctgacctatc ttgtggccta catgtttcgg agcggttccg cgccgatttg tgcgggaccc
      121 tcggcgcggt gagagggcgg cattggggag cggcgcaggg tcgcccgacg ggtgagtccg
      181 gccggcgcaa tgcggccggg tgcgggcagc ggcgccttgc ctttcccctg cttttttctt
      241 accttcacca tacttggaaa aatgctctaa cccccacgaa tcggagaccg cgaatatgtc
      301 ccacaccgcc acccacatcc ccgccgcgcc cacctgggat ctggactcga tcttcccggg
      361 aggcagcgcc tccgccgagt acaaagcgtt ccgcgagaaa gtgaagaaag atctcgccgg
      421 ggccgcgacc gcggtcgacc ggctccccgc cgccatcacc ccggagaacc gcccccagtg
      481 ggcggcggct atttccaccc tccagtccat cctcgccgac ctcgagttgc tgctggcgtt
      541 cgcatcgtgt ctgaaagccc agaatgtcag cgacacgctt gcccacgccg ccgatgccga
      601 aggagaggag gatgtcgccc agtggaagaa gctcatgacc cggatcgagg cgatgtctct
      661 gcagcagtcg gatgcggcct ggcagctgct cacggcggcc ccggaactgc gcgggacgga
      721 gtttttcctc aacgaggtgc gcgagaccgc ccgccggaaa atgccggtgg aaaaagagga
      781 gctggcgctg gatctggcgg tcaacggcta tcacacctgg aaccagttgt atgacaagat
      841 ggccggcgac cttcgggaac cgttcgagga aggcggccag acgaagctgc tctcgatggg
      901 gcagattgcg accaaaatgt ccagccccga ccgggaggtg cggcggcggg cgtttgaggc
      961 gatgaccaac gggtggaagc gccgtcagga tctggcggcg atggcgctca acgcccaggc
     1021 gggattccgc ctgtcgctct acaagtaccg cggctgggat ctcatgtacg aaccgctgcg
     1081 caacgcccgc ctgcaggagg cgacgctcga caccatgtgg cgggtgattg cggaggagaa
     1141 aaagcggctc cgcgcctatg tcgacgcgaa gaagagactg ctggggatcg accggttccg
     1201 ctggtacgac gaattcgcgc cggccggccg gtccgatcgg cagtacagct tcgcggaggc
     1261 cggcgatttc gtcgtggccc aggtgcggtc gttctccgat gacatggcgg atttttaccg
     1321 ccgggcgatc gacagccgct gggtggaggc cgaggaccg
//
LOCUS       DAOOKS010000595         1359 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_129071_length_1359_cov_2.394172, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000595 DAOOKS010000000
VERSION     DAOOKS010000595.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129071_length_1359_cov_2.394172"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="PK112_08820"
     CDS             <1..292
                     /locus_tag="PK112_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPC12184.1"
                     /translation="APLAVLMKSRAFHRVVDATNDSDYGLQAGIFTQRIKDIWYAFER
                     IEAGGVVVNDIPTYRADHQPYGGMKSSGVGREGVRYAIQDQTEVKILSLNLK"
     gene            297..707
                     /locus_tag="PK112_08825"
     CDS             297..707
                     /locus_tag="PK112_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007165197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HPC12185.1"
                     /translation="MTPRSDNPARRRVSSGSPYEEPFGFCRAIRVGNAIHVAGTGPIA
                     ADGSTGAPGDPYGQTMRCLEIIRTAIEQLGGRAADVVRTRIYVTDLTRQDEIGRAHGE
                     FFREINPAATMIGVAGLVRADWYVEIEAEAIVDE"
     gene            786..1145
                     /locus_tag="PK112_08830"
     CDS             786..1145
                     /locus_tag="PK112_08830"
                     /inference="COORDINATES: protein motif:HMM:NF019278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HPC12186.1"
                     /translation="MKFRVIKHHQAAGGPSRELESGERLQFERRPSEYEGWVWCTAED
                     GGGGWVPETWLTITQDPAGGATAGWCFLKRDYSPEELTVHPGDILEGRREESGWLWAC
                     TADGRKGWVPRDAVEPV"
BASE COUNT          237 a          474 c          458 g          190 t
ORIGIN      
        1 cgccccgctc gcggtcctca tgaagtcccg cgccttccac cgtgtggtcg atgcgaccaa
       61 cgactcggac tacggcctgc aggccggcat cttcacccag cgaatcaagg acatctggta
      121 cgctttcgag cggatcgagg cgggcggagt ggtcgtcaac gacatcccca cctaccgcgc
      181 cgaccaccag ccctacggcg ggatgaagag ctccggcgtg ggccgggaag gggtccgcta
      241 cgccatccag gaccagaccg aagtgaagat tctctcgttg aacctgaaat agggtcatga
      301 cgccgcgatc cgacaatccc gcacgccgcc gggtctcctc cggctccccc tacgaggagc
      361 cgttcgggtt ctgccgggcg atccgggtcg gcaacgccat ccatgtggcg ggcacagggc
      421 cgatcgcagc cgatggctcc acgggcgcgc cgggcgatcc gtacgggcag acgatgcgat
      481 gcctcgagat cattcgcacc gctatcgagc aactcggcgg gcgggcggcc gatgtggtgc
      541 gcacccgcat atatgtgacc gacctcacgc gccaggacga aatcggccgc gcccatggcg
      601 agtttttccg cgagatcaac ccggccgcca ccatgatcgg cgtggccggc cttgtccgcg
      661 ccgattggta tgtcgagatc gaagccgagg ctatcgtcga cgagtgagcg ggagcgcggc
      721 aaccgaaggc ccgccgggcg ccgaacacgg cgccccggcg cgcgcgagaa aggagcaacc
      781 gcgacatgaa attccgcgtc atcaaacacc accaggccgc cggcggcccg tcgcgcgagc
      841 tggaatccgg cgaacgcctc cagttcgaac gccgcccgag cgagtacgag ggctgggtgt
      901 ggtgcaccgc tgaggacggc ggcggggggt gggtgccgga aacctggctc acgatcacgc
      961 aagaccccgc gggcggcgcg acggccggct ggtgctttct caaacgagac tacagccccg
     1021 aggaactcac cgtccacccg ggggatatcc tcgagggccg ccgcgaggag tcgggctggt
     1081 tgtgggcgtg caccgccgac ggccgcaagg gctgggtgcc gcgcgacgcc gtcgaaccgg
     1141 tgtaaccgga ttacccagca cggcggcgcg gcgttcgccg gccgcgcctg ccctgtcccg
     1201 ccgccggact acccggccag ccggtcaagc gccgcgcggt caatcgacca ctcgtacccc
     1261 tcgctggaca gctcgaatcc catagcccgg ttcacgagat ggatcggctc gttgaccgcg
     1321 cgcatctcgg tccagatgta gcggctttgc gggaaatcc
//
LOCUS       DAOOKS010000596         1358 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_129195_length_1358_cov_2.696086, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000596 DAOOKS010000000
VERSION     DAOOKS010000596.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129195_length_1358_cov_2.696086"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="PK112_08835"
     CDS             <1..569
                     /locus_tag="PK112_08835"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
                     /protein_id="HPC12187.1"
                     /translation="YRGRLRMCLVERQSRWLTTHGEPYRHAMIGPFAINQELLVPAGE
                     VRELTGTWDGLIAGFANVTRENLLGVAFVTAHDTGYADAATEAPVRLPGEEPEFLRGD
                     ANADGKIDIADAVRVLAYLFGGGQTALVCLAAGDANDDGKIDISDAIKILGHLFAHTG
                     PLPPPFGACGPDPTPDALTCVSFPPCAP"
     gene            674..>1358
                     /locus_tag="PK112_08840"
     CDS             674..>1358
                     /locus_tag="PK112_08840"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPC12188.1"
                     /translation="MPKQRSLKSAALSRRGFMARSAAVTSAAAAAAALPVHAAGSDML
                     RIAMVGCGDRGTHDAIVLLQSVPGVELYAMADMFQDRIDASLAELRRRVPGKVNVTKE
                     RTFLGFDAYKKVLALGEIDIVMYLTPPGFRPEHVAETVKAGKHMFLEKPGAVDAVGIR
                     ALLAASEEADKKRLSIVVGTQQRYAPQYLDVLDLIWSGRIGELRVLKAHWICEMVDWH
                     FHPHRPEWSD"
BASE COUNT          208 a          497 c          432 g          221 t
ORIGIN      
        1 actatcgggg tcggctgcgc atgtgccttg tggaacgtca atcgcgctgg ttaaccacgc
       61 acggcgagcc ctaccgccac gccatgatcg gccccttcgc catcaaccaa gagttgctcg
      121 tgcccgccgg cgaagtccgc gagctcacgg gaacctggga tggtttgatc gccgggttcg
      181 ccaacgtcac gcgggagaat ctgctgggcg tcgccttcgt gacggcccac gacaccggct
      241 acgccgatgc cgcgaccgag gccccggtca ggttgccggg cgaggagccg gaattcctgc
      301 gcggcgacgc caacgccgac ggcaaaatcg atatcgccga tgcggtcagg gtgctcgcgt
      361 accttttcgg cggcggccag acggcgctgg tttgcctggc cgccggcgat gccaacgacg
      421 acggcaaaat tgacatctcc gacgcgatca agattctcgg ccacctcttc gcgcacacgg
      481 gaccgctgcc gccgcccttc ggcgcctgcg gccccgatcc cacccctgac gccttgacct
      541 gcgtttcgtt ccccccctgc gcgccctagg cccttgaccc cgccgcgcgc ccgcggctac
      601 actcgggttg tcgctgcggc gctcccgcgc gcgaaacgcg cggtcgaagc gccgcgcatt
      661 cgcgaggtgc cccatgccga aacagcgttc gctcaagtcc gccgctctct cccgccgcgg
      721 tttcatggcc cggtcggccg ccgtgacgag cgccgcggcg gcggccgccg ccctccccgt
      781 ccacgccgcg gggtcggaca tgctccgcat cgccatggtc ggctgcggcg accgcggcac
      841 acacgatgcc atcgtactgt tgcaatcggt gccgggcgtc gagctctacg ccatggccga
      901 catgttccag gaccggatcg acgcgtccct ggcggagttg cgtcggcgcg ttcccggcaa
      961 ggtgaacgtc accaaggagc gcacattcct cggcttcgac gcctacaaga aggtgctggc
     1021 cctcggggag atcgatatcg tcatgtattt gacgcctccc ggtttccgtc ccgagcacgt
     1081 cgccgagacc gtgaaagccg gcaagcacat gttccttgag aagccgggcg ccgtggacgc
     1141 ggtgggaatt cgcgcgctgc tggcggcctc cgaggaagcc gacaagaagc gcctctccat
     1201 cgtcgtcggc acccagcagc gctacgcgcc gcagtacctc gacgtcctcg atctcatctg
     1261 gagcggccgc atcggcgagc tgcgcgtcct gaaggcccac tggatctgcg agatggtcga
     1321 ctggcacttc catccgcacc ggcccgagtg gagcgaca
//
LOCUS       DAOOKS010000597         1346 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_130904_length_1346_cov_3.441518, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000597 DAOOKS010000000
VERSION     DAOOKS010000597.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130904_length_1346_cov_3.441518"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="PK112_08845"
     CDS             <1..262
                     /locus_tag="PK112_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HPC12189.1"
                     /translation="EDVDAIGLSILSGAHMTLFPAILEEMKKKGAGDIILFGGGIIPE
                     DDKAALENMGVGKIFTPGAPTEAAIEYLREAVAKKQKGEQIM"
     gene            370..>1346
                     /locus_tag="PK112_08850"
     CDS             370..>1346
                     /locus_tag="PK112_08850"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPC12190.1"
                     /translation="MNRNIDPRQQAVRDQVKQFAEKEIYPITRELDQMPEPRRFPTEL
                     YRKIGAAGFIGYAMPKELGGSGKSHLEYITLVEELCYHDPAVGLLCAVGELATHPIIH
                     FASDQQKKKYVPDCARAARIPAFVLTEPNAGSDAANQQTEAVEGDGGFILNGEKIFIM
                     HGDAADLAVVFCKVKDKPKLSAFIIDDLKQPGWQARTLRQKMGMRAATTGGIHLKNVF
                     VPKENLLGEFGKGFRYAMTTLDSARVGVAAQGVGIAQRMLDEAVAYAKKREAFGAPIA
                     KLQAIQWMIADMATRLAAARHLTYHAAKLQDAGESFGLEASMAKLYCSET"
BASE COUNT          269 a          481 c          403 g          193 t
ORIGIN      
        1 ggaggatgtg gacgccatcg ggctcagcat cctctccggc gcccacatga ccctctttcc
       61 tgcgattctc gaggaaatga agaagaaggg ggccggggat atcatcctct tcggcggcgg
      121 catcatcccg gaggacgaca aagccgcgct cgagaatatg ggcgtcggca agatcttcac
      181 ccccggtgct ccgaccgagg ccgccatcga gtacctccgc gaggcggtcg cgaagaaaca
      241 gaaaggcgag cagatcatgt aggccgggtt ccgagggccg gccgcccgcg gcgacccggc
      301 ggcccgccga tgaccccctg cgaatacagg cacaatgtca ctctataaca accccgtcag
      361 ggaggaccca tgaacaggaa tatcgacccg cggcaacagg ctgtccgcga ccaggtcaag
      421 cagttcgccg agaaggagat ctacccgatc acgcgcgaac tcgaccagat gcccgaaccg
      481 cgccgcttcc ccaccgagct ctaccgcaag atcggcgccg ccggcttcat cggctacgcc
      541 atgcccaaag aactcggcgg ttccggcaag tcgcacctcg agtacatcac cctggtggag
      601 gagctctgct accacgaccc ggccgtaggc ctcctctgcg ccgtcggcga actcgccacc
      661 cacccgatca tccacttcgc ctccgaccag cagaagaaga agtatgtgcc cgactgcgcc
      721 cgcgccgcgc gcatccccgc gttcgtgctc accgagccga acgccggttc cgacgccgcc
      781 aaccagcaga ccgaggcggt cgagggggac ggcggcttca ttctcaacgg cgagaagatc
      841 ttcatcatgc acggcgacgc ggccgacctg gccgtggttt tctgcaaggt caaggacaag
      901 cccaaactct ccgccttcat catcgacgat ctcaagcagc ccggctggca ggcccgcacc
      961 ctccggcaga aaatggggat gcgcgcggcg accaccggcg gcatccacct caagaacgtc
     1021 tttgtcccca aagagaacct gctcggcgag ttcggcaagg gtttccgcta cgccatgacc
     1081 accctcgact cggcccgtgt cggcgtggcc gcccagggcg tcggcatcgc ccagcggatg
     1141 ctcgatgagg ccgtggccta cgcgaagaag cgcgaggcct tcggcgcgcc gatcgccaag
     1201 ctgcaggcga tccagtggat gattgccgac atggccaccc ggctggcggc ggcccgccac
     1261 ctgacctacc acgccgctaa actgcaggac gccggcgaat cgttcgggct ggaggcctcc
     1321 atggccaagc tctactgctc cgagac
//
LOCUS       DAOOKS010000598         1343 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_131344_length_1343_cov_5.155280, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000598 DAOOKS010000000
VERSION     DAOOKS010000598.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131344_length_1343_cov_5.155280"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            570..713
                     /locus_tag="PK112_08855"
     CDS             570..713
                     /locus_tag="PK112_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12191.1"
                     /translation="MRSALSLDGIECYPNVEVTPSVTVSLMAEIDRVIEARGGLPGAF
                     KAD"
     gene            838..>1343
                     /locus_tag="PK112_08860"
     CDS             838..>1343
                     /locus_tag="PK112_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12192.1"
                     /translation="MSEHFLSALRAQLRAHGQAPLRVIGIDLGTTNCTVAEIVADGAA
                     EDFEPRCIEIEQETPFQGEYTHILIPSIVALHGGREYVGEGAKRLRAGLAGPETGLER
                     NRTIFWEVKNEMGIQRTYARAPEGHRGAAEVSGRILRFLANRYRADHGEPRKAVVTVP
                     ASFQQPQR"
BASE COUNT          249 a          419 c          471 g          204 t
ORIGIN      
        1 acgaccgaat ggccgccgcg gcgccgcccg cggaagcgat cgccgaggcg caacgcctga
       61 tgatccattc cgatcgcccg ctctaccgcc atccgtattt ctgggcttcc tacgtgctta
      121 cccagacggg cgctcggaag accaagtaag aacagtgcgc ggagtcgctc cgggtgacct
      181 tgcccggcct ccggcagacc tgcatggtcg gcggctctct gcgggggcac attcaggacc
      241 cgtcttatga ctcgcaggac ggccgtgtgg aggtcggctc gccccgcagc actcccggag
      301 cggcccaaaa acccttgcga tttgactcgc ccggcgctat cttcgaaccc ggtggcggcg
      361 ctaagcacag ccgatctgga agaggttcgg ttggggagac aacacggcgt tgtgacacgg
      421 cgattgaagg ctatcaccga tgagcggagt gggacgacgg ggcggcgagc ttttcattgt
      481 cgaaaacggc gattccgacc ggaaagcctg gcagtgtctc cacgactggg ccgagacagc
      541 tgacaagctc gatagtgcga cggggtactg tgagatcggc gctctccctg gacggtatcg
      601 aatgctaccc gaatgtcgag gtcacgccga gcgtgaccgt ctccctcatg gccgagatcg
      661 acagagtgat cgaggcccgc ggcggcttgc cgggggcgtt taaggcggac tagcggggcc
      721 gcacactatc ggactggaac gcgggcattc tccggacagc agggggagac acaacgatga
      781 gcagcgcccc gctccggcgg gcgaacaacc gaacctgtga caaggggacc gggacatatg
      841 agcgagcatt ttctctccgc cctgcgggcg cagctgcgcg cgcacgggca ggcgcccctg
      901 cgcgtcatcg gcatcgacct gggcaccacc aactgcacgg tcgcggagat tgtcgccgac
      961 ggcgcggctg aggatttcga gccgcggtgt atcgagatcg agcaggagac gccgttccag
     1021 ggtgagtaca cgcacatcct gataccctcg atcgtcgcgc tccacggcgg ccgcgagtat
     1081 gtcggcgagg gggccaaaag gctgcgggcc ggcctcgccg gcccggagac cggcctggag
     1141 cgcaatcgga cgatattctg ggaagtgaag aacgagatgg ggatccagcg cacctacgcg
     1201 cgcgcgccgg aggggcatcg cggcgcggcc gaagtgagcg gtcggattct gcggtttctg
     1261 gccaacaggt accgggcgga ccacggggag ccgcgcaagg cggtggtgac ggtgccggct
     1321 tcgttccaac agccgcagcg aga
//
LOCUS       DAOOKS010000599         1340 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_131922_length_1340_cov_2.346304, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000599 DAOOKS010000000
VERSION     DAOOKS010000599.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131922_length_1340_cov_2.346304"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(203..874)
                     /locus_tag="PK112_08865"
     CDS             complement(203..874)
                     /locus_tag="PK112_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006987115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPC12193.1"
                     /translation="MLRHIALTGICVVLIAALVGCGMSKTQKGALIGAGSGAVVGGLV
                     GNAAGNTAVGAIIGAAVGGAAGAYIGHYMDQQAEEMQKDLEGARIERVGEGIKITFAS
                     GILFDVNKADLRPEAQQNLVNLAAILNKYAETNVLIEGHTDTTGSDSYNLTLSRSRAE
                     SVSSFLAAQQVVSNRFIIQGYGEQQPIATENTAAAMQANRRVEIAIYANDKLKKAAEE
                     QVQEG"
BASE COUNT          268 a          375 c          417 g          280 t
ORIGIN      
        1 tctccggagg ccggggcgag cggggcgcat agggataacc ccggacttac gccgcccgcc
       61 gggtggacag ccgccggcgg caaggctggc cgggcaggag gggggccgcc gggcagtaaa
      121 gctgtcaaga ggtgaaccca aaaagagaaa gggacagctt ggcagctgtc ccttggatcc
      181 atcaatcctc ggccggagcg cgtcagccct cttgcacctg ctcctcggcc gctttcttca
      241 gtttgtcatt ggcgtagatg gcgatctcta cgcgccggtt ggcctgcatg gcggccgcgg
      301 tgttctccgt ggcgatcggc tgctgttcgc cgtaaccctg gatgatgaag cggttgctca
      361 ctacctgctg ggcagcgagg aaactcgaga ccgactccgc gcgggagcgg gagagcgtga
      421 ggttgtagga atccgatccg gtcgtgtcgg tgtgcccctc gatgaggaca ttggtctcgg
      481 cgtacttgtt gagaatggcg gcgaggttca cgaggttctg ctgggcctcg ggccgcaggt
      541 cggccttgtt cacgtcgaaa aggattccgg aagcgaacgt aatcttgatg ccttcgccga
      601 cacgctcgat ccgcgcccct tcaaggtcct tctgcatttc ctcagcttgc tgatccatgt
      661 agtggccgat ataggcgccg gccgcgccgc caacggcggc gccgatgatc gcgccgaccg
      721 ccgtgttgcc ggcggcgttg ccgactagtc cgccgaccac ggcccccgac ccggcgccga
      781 tcagagcccc tttctgcgtt ttgctcatac cgcagccgac aagggcggcg atcagcacca
      841 cgcaaatccc ggtcaaagca atgtgcctaa gcatgagtcg ctactccttg aacaattggg
      901 ttatgccata tgccaagcta cccgtactac acatcgggag attcgcatgc gaatccaact
      961 aaaggatggc gaattccggg gtggcgtcca agtttttttt cggactccgg actaaccagc
     1021 ttgactgccg ggaggctgac tgccggattg gcggttgagc tggccagttg acagactatc
     1081 accgcctatt tggcagccat gctgacattc ttggcaggtt ggtcatcgga acgagcagat
     1141 tcgttatagt gatattgttg tcaatcatgt gcttatgtgg aatggcacgg aagttgataa
     1201 tcacttatga cagaagtcac agaaggaatc gagaaacaga gagacgaaat aaggaggttc
     1261 cagaaatgcg tagcgtgatg attccgaatc ggttggcgcg ggaaatcgac agcatgttca
     1321 acaccttcat cagttttccg
//
LOCUS       DAOOKS010000600         1323 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_134556_length_1323_cov_2.373817, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000600 DAOOKS010000000
VERSION     DAOOKS010000600.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134556_length_1323_cov_2.373817"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..702)
                     /locus_tag="PK112_08870"
     CDS             complement(94..702)
                     /locus_tag="PK112_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPC12194.1"
                     /translation="MTDGKAKTGDDKRKELEKRRAFEKEALPHMDALYRTALRMTKNN
                     NDAEDLVQEAYVKAYRFWDRFEPGSNCRAWLFKIMTNIFINDYRSKSRSPMSVNVDEI
                     DDSFLYGQLAATAPSDNPEKELFAKIFDDDVKRAIEGLPEDFRLVVVLSFLEGFSYQE
                     IADIADLQLGTVKSRLHRGRKLLQKALYDYAIKNGYIKDTAR"
     gene            complement(1072..>1323)
                     /locus_tag="PK112_08875"
     CDS             complement(1072..>1323)
                     /locus_tag="PK112_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12195.1"
                     /translation="AGDRPATARDLPLGRLPAEEGVHYEYEEEEALRVGRLVRHPTFG
                     RGKVLKVEGRGESLMLEIHFQGLGVKRVMAKYARLTVIG"
BASE COUNT          249 a          415 c          348 g          311 t
ORIGIN      
        1 atctttcgag gtgttctctg acctgtctgg tgtcgatttc cgaggcttct ttatcgatta
       61 tgtcgtacaa caggttcagc gcttcctgac aattcatctg gccgtatcct tgatgtagcc
      121 gttcttgatt gcgtagtcat acaaggcttt ttgaagcagt ttcctccctc ggtgcaggcg
      181 cgatttcacc gtccctagct ggagatcggc aatatcggcg atctcctgat acgaaaaccc
      241 ttcgaggaac gacagcacga cgacgaggcg aaaatcctcg ggcaaccctt cgatggcgcg
      301 tttcacgtca tcgtcgaaaa tcttcgcgaa caactctttc tcgggattgt cgctcggggc
      361 ggtggccgcg agctgaccgt agagaaagct gtcgtcgatt tcgtcgacat tgaccgacat
      421 cggggagcgg gacttggagc ggtagtcgtt gatgaaaatg ttggtcatga ttttgaacaa
      481 ccatgcccga caattcgacc ccggctcaaa ccgatcccag aagcgatacg ccttgacata
      541 cgcctcctgc accagatctt cggcgtcgtt gttgttcttg gtcatccgaa gagcggtgcg
      601 gtagagggcg tccatgtggg gcagcgcctc tttctcgaat gcccgtcgct tctcgagttc
      661 cttcctctta tcgtcaccgg ttttcgcttt accgtccgtc atggaaacca tatctcactt
      721 ccccgcgacc ggtaaagcaa gccattgcct atacttagaa acaacggctg cggtcgcgaa
      781 agttccctgg ttgcaccctc tgttcccggc cgccggctga tctccatcct tattgttgag
      841 agactccctg ggcgtgggcc ggccgccaaa ggctaatccc atccaccaat tcaatataag
      901 ccgcccgcat gccccctaca agagaattcg gaccgacctt ggtcggttcg acggttccgg
      961 catcctgaaa gttgtaccac ccagaaggcc gaccgccgag aatatcggga tagtttccat
     1021 ggagattgcc ctcaacctcc cccaggggcc tgcctcccgc gccttccggc ctcagccgat
     1081 gacggtcagc ctggcgtact tggccatcac tctcttcacc ccgagtccct ggaaatggat
     1141 ctcgagcatc agcgactcgc cgcgcccttc gactttgagc actttgcccc gtccgaaagt
     1201 agggtggcgc acgagccgtc cgacgcgcag cgcctcctcc tcttcgtact catagtggac
     1261 cccttcctct gccggaagcc gtcccaacgg gaggtcgcgg gccgtggcgg ggcggtcgcc
     1321 ggc
//
LOCUS       DAOOKS010000601         1318 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_135340_length_1318_cov_2.642914, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000601 DAOOKS010000000
VERSION     DAOOKS010000601.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135340_length_1318_cov_2.642914"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..814)
                     /locus_tag="PK112_08880"
     CDS             complement(<1..814)
                     /locus_tag="PK112_08880"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPC12196.1"
                     /translation="MLDVDWYRAARAAWERNPGQEMPEFNAAIEALGRQADTMIFVAA
                     DDKEIIRAARLAREFGRTFICVGSGCEYERAEEIRGELVELIIPVNYPEAPEVGSLED
                     ELEVTLEDLRHWERAPGNAARLAQEKIPFAFTAAGLDDPSEYLSRVRTAVRYGLPPET
                     ALRALTTVPARLCGVDRYVGTLEPGKLANLVVCSGDLFADTTRILAVWAAGQKYEFEP
                     LHPGEYRGQWQLRTAADSATLALEGAPSKLAGRLVAGSDSVELGEIEAARDRI"
     gene            complement(879..>1318)
                     /locus_tag="PK112_08885"
     CDS             complement(879..>1318)
                     /locus_tag="PK112_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPC12197.1"
                     /translation="VIVFEHGVIREVSRSARVPEGALVVDLGGRVVYPGFIDPHSDYG
                     LLAEEEGHGQGGRIAWGGPKYEADRIGGSAWNEAIRAEIDWVDSFRPDSTAAQKYLGA
                     GITVTQTTRQEGIFRGRPAVVLLRQGLPNNQVLRARSYPALSF"
BASE COUNT          213 a          455 c          423 g          227 t
ORIGIN      
        1 taatgcggtc gcgggcggct tcaatctcgc ccagctccac ggaatcggag ccggccacca
       61 gtcggcccgc gagtttcgat ggtgcacctt ccagggcgag tgtcgcgctg tccgccgcag
      121 ttcgcagttg ccactgaccc cggtattccc ccggatgcag cggctcgaac tcgtatttct
      181 ggccggccgc ccagacggcc aggatgcgcg tcgtgtcggc gaacaggtcg ccgctgcaca
      241 caacgaggtt ggccagcttc cccggttcga gggtaccgac ataccggtct actccgcaga
      301 gccgcgcagg gaccgtcgta agcgctctca gggccgtttc cgggggcaga ccgtaccgca
      361 cggccgtgcg gacgcgggag aggtattcgg aggggtcgtc caagccggcc gcggtgaaag
      421 caaacgggat cttttcctgg gccaggcgcg cggcgttgcc gggggcgcgc tcccagtgcc
      481 ggagatcctc cagggtgacc tcgagttcgt cctccagaga gcccacttcc ggcgcttccg
      541 ggtagttgac cggaatgatc agctccacca gctcaccgcg gatttcctca gcccgctcgt
      601 attcgcaccc gctgccgaca caaatgaagg tccgcccgaa ctcccgggca agccgtgcgg
      661 cccggatgat ttctttgtca tcggccgcca cgaaaatcat ggtgtcagcc tgccgtccca
      721 gagcctcgat ggccgcgttg aattcgggca tttcctgacc ggggttgcgt tcccaggccg
      781 cccgcgcggc ccgataccag tctacgtcga gcaatgtttg ccgaatcagg gcgatagccc
      841 ccatttgcga agaggggtaa tcctgcgccg atgagccctt agaacgacag cgccggatag
      901 gagcgggccc gcagcacctg attgttgggc agcccctgcc gcaggagaac cacagccggg
      961 cgtccccgga agattccctc ctgtcgggtc gtttgcgtca ccgtgatgcc cgccccgagg
     1021 tacttctggg ccgccgtcga gtcgggtcgg aaggaatcga cccaatcgat ctcggcgcgg
     1081 atcgcctcgt tccaggcgct gccgccgatc cggtcagcct catacttggg accgccccag
     1141 gcgattcggc cgccctggcc gtgtccctct tcctcggcga gcaggccgta gtcggagtgg
     1201 ggatcgatga atccggggta gaccacgcgg ccgccgaggt caaccaccag cgctccctcg
     1261 ggtacgcgcg ccgaccggct gacctcgcgg atgacaccgt gctcgaacac gatcaccg
//
LOCUS       DAOOKS010000602         1317 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_135492_length_1317_cov_3.083201, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000602 DAOOKS010000000
VERSION     DAOOKS010000602.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135492_length_1317_cov_3.083201"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1317)
                     /locus_tag="PK112_08890"
     CDS             complement(<1..>1317)
                     /locus_tag="PK112_08890"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12198.1"
                     /translation="HFQGPLPVYVALPTPMPHDAIPTKAITVAVWVNHDPMTTDQMEI
                     FMPMSGGEYQKQLGHFELRNNNTARFLLRTPVDPPEDFINMNNVGAVPAKEWVHYAAT
                     YDSVDGVGRLYINGEIVGEAFATREMYNNWDTGARVGYCVDNARPFFGYMDDFAIWSR
                     ALSQDEIYVLMDKSIPVPPPPIEITIDGDFSDWAYLPALIKDPADVFEPNGDIKAVYA
                     HSTADTLYARIAVYGTAAPRDAQRYYYHILIDADNDPATGFDNSEYEGNPTGVVETIG
                     ADFYIMIGRKNGADDGLKIYFCRAGGYEKEIAWDVLYAASGDSIELAVPYASLVPEDD
                     LGAIFKPGQTFKIAAFQEGNANDWEVDWTESAEVRIPPAGNMPPVVSIEPAAAAVALC
                     GESVSCSLTVTAVDPENDPLTYQWSVSGSATIVPGDPPATAVAAFTA"
BASE COUNT          217 a          409 c          439 g          252 t
ORIGIN      
        1 gccgtgaagg cggcgacggc cgtcgcgggc ggatcgccgg ggacgatcgt cgcgctgccg
       61 ctcaccgacc attgatacgt taggggatcg ttctccgggt cgacggcggt caccgtcagg
      121 ctgcagctca cgctctctcc gcaaagcgcc accgcggcgg cggcgggctc gatgctgacg
      181 accggcggca tgttgcccgc cggcggaatt cgaacctccg ccgactcggt ccagtcgacc
      241 tcccagtcgt tggcgttgcc ctcctggaac gccgcgatct tgaaggtctg gcccggcttg
      301 aagatggcgc ccagatcgtc ctcagggacc agcgaggcat agggcacggc cagctcgatg
      361 gagtcgccgg aggcggcgta aaggacgtcc caggcgatct ctttctcgta ccccccggcc
      421 cggcagaaat agattttcag gccatcgtcg gcgccgttct tccggccgat catgatgtag
      481 aagtcggcgc cgatcgtctc gacgacgccg gtggggttgc cctcgtactc ggagttgtcg
      541 aagccggtgg cgggatcgtt gtcggcatcg atcaagatgt ggtagtagta gcgctgcgcg
      601 tcccgcggcg cggccgttcc gtaaaccgcg atccgcgcgt agagagtgtc cgcggtgctg
      661 tgggcataga ccgccttgat gtcgccgttg ggttcgaaga cgtcggcggg atccttgatc
      721 agcgccggaa gataggccca atcggagaaa tccccgtcga tggtgatctc gatgggcggc
      781 ggaggcaccg gtatgctctt atccatgagc acatagattt catcctggga cagcgcgcgg
      841 ctccagatgg cgaagtcatc catgtacccg aaaaagggcc tggcattgtc gacgcagtac
      901 cccacccggg cgccggtgtc ccagttgttg tacatctcgc gggtcgcgaa ggcctcgccc
      961 acgatctcgc cgttgatgta cagccggccc acgccatcca cgctgtcgta ggtggccgca
     1021 taatgcaccc actccttggc gggcaccgcc cccacgttgt tcatgttgat gaaatcctcc
     1081 ggcgggtcga cgggggtcct gagcaagaac cgcgccgtgt tgttgttcct gagttcgaag
     1141 tggccgagct gcttctggta ctccccgccg ctcatgggca tgaagatctc catctggtcg
     1201 gtcgtcatgg gatcgtggtt gacccagacg gccacggtga tggccttggt gggaatcgcg
     1261 tcatgcggca tcggcgtggg cagggcgaca tagaccggca acgggccctg aaaatgc
//
LOCUS       DAOOKS010000603         1314 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_135985_length_1314_cov_3.056394, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000603 DAOOKS010000000
VERSION     DAOOKS010000603.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135985_length_1314_cov_3.056394"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            213..>1314
                     /locus_tag="PK112_08895"
     CDS             213..>1314
                     /locus_tag="PK112_08895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPC12199.1"
                     /translation="MANAINLLVLTHNYPRRAGDHAGVFIALLAKHLEPHGLRPIVLA
                     PHDPGAAEREDLEGVRVHRFRYAARDQDEDVAYRGHMQHIVLGSVSGVFRFRRFLHCW
                     RAAAFDLLVRERIDAVAGHWLVPSGFVLKTIADRTTLPLVLSSHGTDVRLIRKFAGAA
                     YRYFRPLGRRLDRWTFVSTFLRDAIAGLDPGLAPILEVLPLPHDETVFHRDPAVPREP
                     HTIVAVTRFTPQKRVDALVKAFALVAAHRPDTRLEIYGAGPGEHGIRALIDRFGLGER
                     VRISPPIPQDRLRTVYNRAGMVVLNSHEEGFGLALSEAMLCGAPVIGAASGGITDIIA
                     HERRGLLVEPDNTAALAEAILRLLADHPLRARL"
BASE COUNT          194 a          519 c          378 g          223 t
ORIGIN      
        1 tggaaggcct cgtaccggtc gtcctcccac acccgggccg cgatatccag aatcgggttg
       61 gtcagaatct cgcgaaaatc gagatcgagc gcgaaatcta tcggagtccg ggtcttcata
      121 gtcaccgccg agtaagccgg agtataacac ataagcgcgc cgcccgaaaa ttatatttgg
      181 cgcggccgcg gcccttttcc tactctgcgg tgatggcgaa cgcgataaac ctgctcgtcc
      241 tgacccacaa ctacccccgc cgggccgggg atcacgccgg cgtgttcatc gccctcctgg
      301 cgaaacacct ggagccccac ggtctgcgtc ccatcgtgct tgccccccac gatcccggcg
      361 ccgccgagcg cgaggacctc gagggcgtgc gcgtgcaccg cttccgctac gctgcccggg
      421 atcaggacga ggacgtcgcc taccgcggcc acatgcagca catcgtcctc ggctcggtat
      481 cgggcgtctt ccggttccgg cgcttcctcc actgctggcg cgccgccgcg ttcgatctgc
      541 tcgtgcgcga gcgcatcgac gcggttgccg gccactggct cgtcccctcc gggtttgtcc
      601 tgaaaacaat cgccgaccgc accaccctgc cgctcgtgct ctcgtcgcac ggcaccgatg
      661 tgcgactgat ccgcaagttc gccggcgccg cctaccgcta tttccggccg ctcggccggc
      721 ggctcgaccg ctggactttc gtctcgactt tcctccgcga cgctatcgcc gggctcgatc
      781 ccgggctggc gccgattctc gaggtcctcc cgctgccgca tgacgagacc gtattccacc
      841 gcgacccggc cgtcccgcgc gaaccgcaca cgatcgtcgc cgtcacccgc ttcacccccc
      901 agaaacgcgt cgacgccctc gtgaaagctt tcgctctcgt cgccgcgcac cgccccgaca
      961 cccgcctgga gatatacggc gccggtccgg gtgaacacgg gatccgcgcc ctgatcgacc
     1021 gtttcggcct gggcgagcgc gtacgcatct cgccgcccat tccccaggac cgcctccgca
     1081 ccgtctacaa ccgggccggc atggtggtcc tcaactcgca cgaggaggga ttcgggctgg
     1141 cgctctcgga ggcgatgctc tgcggcgccc ccgtgatcgg cgccgcttcc ggcggcatca
     1201 ccgacatcat cgcccacgaa cggcgcggcc tgctcgtcga acccgacaac accgccgccc
     1261 tcgccgaggc gatcctccgc ctactcgccg accatcctct gcgcgcccgc ctcg
//
LOCUS       DAOOKS010000604         1313 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_136126_length_1313_cov_3.156598, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000604 DAOOKS010000000
VERSION     DAOOKS010000604.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136126_length_1313_cov_3.156598"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..175
                     /locus_tag="PK112_08900"
                     /pseudo
     CDS             1..175
                     /locus_tag="PK112_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063501.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate mutase L"
     gene            complement(272..427)
                     /locus_tag="PK112_08905"
                     /pseudo
     CDS             complement(272..427)
                     /locus_tag="PK112_08905"
                     /inference="COORDINATES: protein motif:HMM:NF012305.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
     gene            complement(612..>1313)
                     /locus_tag="PK112_08910"
     CDS             complement(612..>1313)
                     /locus_tag="PK112_08910"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HPC12200.1"
                     /translation="LVEELVRHVAFAVHGAYRFTYGEREIDVAPPFRWVSMVDSIREA
                     AGLDLMALSYEQARAEAKKMHLPDEDLTNWGKCVEAIWEAKVEPTLVQPTFIADFPRE
                     ISPLAKVHRENPRLAERFELFIAGQETANAFSELNDPVDQLRRFIEQGKALAAGDEEA
                     QPLDDDFITALTYGMPPTAGLGLGVDRFVMLLTNQHTIREVLLFPQMKRELADGAVPV
                     SRILARIIGADKETP"
BASE COUNT          220 a          448 c          419 g          226 t
ORIGIN      
        1 cgtccgcgac atgaccgtgc gggcggcgcg cgaggcggcc ctgggggcgg gggccatcct
       61 caagtacatc accgccgggc ggctgcgccg caccgaggcc gccaggatcc gcgagatcgc
      121 cccgaacata atcctggtcg ccggcggggt ggactacggc gagcgcgaca ccgcccccgc
      181 cgccttcgcg cggacgggct tcgctgagcg tcagggcgcg gccgccgtag tcgctcccct
      241 ggaggccgtc aatcgccgcc tgggcctctt cgtcattcgg catttcgaca aacgcaaacc
      301 cgcgggaccg accgctcacc cggtccatga tgatcttggc cgaggtcacg gtgccgtacg
      361 cctcaaactt cgccttcagc tcctcttcat cagtgctgaa cggaagattg ccaacgtaga
      421 tattcatcgc atcaaactcc aaaaaaagac tagttgccgg ttttcatgtt cccccgctgc
      481 gcgcacatca ggtcataggg cactcctccc aagcgacacc gttaaccgtt gatcgggtct
      541 gcagcgggga caggcgacgt gcggaggcgg actgcagacc ggggccacca tgtatctcac
      601 cttttgcacg gtcacggggt ctccttgtcg gcgccgatga tccgggccag gatgcgcgac
      661 accggcaccg cgccgtccgc cagctcccgc ttcatctgcg ggaacaggag cacctcgcgg
      721 atcgtgtgct ggttggtcaa gagcatgacg aagcggtcga cgcccaggcc gagcccggcc
      781 gtcggcggca tgccgtaggt gagggcggtg atgaaatcgt catcgagcgg ctgcgcctct
      841 tcgtcgccgg cggccagcgc ttttccctgc tcgatgaacc gccggagctg gtcgaccggg
      901 tcgttgagtt cggagaaggc gttggcggtc tcctggccgg cgatgaacaa ctcgaaccgt
      961 tcggccaaac ggggattctc ccggtggacc ttggccagcg gcgaaatctc ccgcgggaaa
     1021 tcggcgatga aggtcggctg cacgagcgtc ggttccactt ttgcttccca gatcgcctcc
     1081 acgcacttcc cccagttggt caggtcctcg tcgggaaggt gcatcttctt cgcctcggct
     1141 ctcgcctgct cataagacag ggccatcagg tcgaggccgg ccgcctcccg gatcgaatcc
     1201 accatcgaca cccaccggaa cggcggggcg acgtcgatct cccgctcgcc gtaggtgaag
     1261 cggtaggcgc cgtggacggc aaaggccacg tggcgcacca gctcttcgac cag
//
LOCUS       DAOOKS010000605         1312 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_136302_length_1312_cov_3.447892, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000605 DAOOKS010000000
VERSION     DAOOKS010000605.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136302_length_1312_cov_3.447892"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="PK112_08915"
     CDS             <1..366
                     /locus_tag="PK112_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12201.1"
                     /translation="ALIAGLPNLVTVLLGYLLLTGRSVFARIAPLAGRSLAYWATLIF
                     LLADPLNIAVGPFLYGGDAGGIAHGLGLNQYFLQALAFGVFLWNRELVASRLLPAFGV
                     VTSHPLFRPWLSGGPAAGE"
     gene            465..>1312
                     /locus_tag="PK112_08920"
     CDS             465..>1312
                     /locus_tag="PK112_08920"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPC12202.1"
                     /translation="MVDRSRGFFDRWGLLVVLAVALLVRVVYLFQYHATGDWSQLTVD
                     ANYHHHWAQDIAAGDVFGDTTYFRAPFYVFCLALLYAVLGDSLWIGRLFGLAIGLASV
                     ALTSLLARRLFGAKAGFFAGLVHALCMMAVYFEPELLVDPLYTLLLLAALHGFVTWYD
                     SRAVRDALTFAVLLGLAAITRPNALVMVPVAVLVLAVRWRRIAAPVPHLGALALGLLL
                     VIGPITLRNYLKANDPALIATSGGINLYIGNNPEADGAAAVLPPPMGPYWRLERTEYI
                     AESDLGR"
BASE COUNT          148 a          489 c          393 g          282 t
ORIGIN      
        1 gcgctcatcg ccggcctgcc caacctcgtg accgtgctac tgggctacct gctgctgacc
       61 gggcggagcg tattcgcccg aatcgcgccg ctggccgggc gttccctcgc ctactgggcg
      121 actctcattt ttctgctggc cgatccgctg aatattgcgg tcgggccctt cttgtacggg
      181 ggggacgccg gcgggatcgc ccatggcctc ggcctcaacc agtactttct ccaggcactc
      241 gcgttcggcg tcttcctgtg gaaccgggaa ctggtcgcct cccgcctctt gccggcgttc
      301 ggggtcgtca ccagccaccc ccttttccgc ccctggcttt ccggcgggcc agcggccggt
      361 gaatagagcc ggcggccgcc gcctgcggcc ccggccgcag gggcggtccg ggccgcgccg
      421 aatttgatgc atgcgttcct ttaacaactg gcctatattg cggcatggtt gatcggtcgc
      481 gaggattttt cgaccgctgg gggcttctcg tcgtcctcgc tgtcgccctc ctggtgcgcg
      541 tggtctatct cttccagtac cacgccaccg gcgactggtc gcagctcacg gtcgacgcca
      601 actaccatca ccactgggcg caggatattg ccgccggcga tgtcttcggc gacaccacct
      661 atttccgcgc ccccttctat gtgttctgcc tcgccctcct gtatgcggtc ttgggcgact
      721 ctctctggat cgggcggctc ttcggcctgg cgatcggcct ggcctcggtc gctctcacca
      781 gtctgctggc gcgccggctc ttcggcgcga aagccggctt tttcgccggc ctggttcatg
      841 ctctctgtat gatggccgtc tatttcgagc cggaactgct ggtcgatccg ctctacaccc
      901 ttctcctgct ggcggcgttg cacggcttcg tgacctggta tgactcgcgc gccgtccgcg
      961 atgccctcac gttcgccgtc ctcctcggcc tcgccgccat cacccggccc aacgccctcg
     1021 tgatggttcc ggtggcggtt ctcgtgctgg cggtccggtg gcgccgcatc gccgccccgg
     1081 tcccccacct cggggcgctc gccctcggtt tgctcctcgt catcggcccg atcacgctcc
     1141 ggaactacct caaggccaat gatcccgctc tcatcgccac ctcgggcggc atcaacctct
     1201 acatcgggaa caaccccgag gccgacggtg cggcggcggt gctgccgccg cccatgggtc
     1261 cctactggcg gctcgaacgg accgagtata tcgccgaaag cgacctcggc cg
//
LOCUS       DAOOKS010000606         1307 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_137065_length_1307_cov_3.623802, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000606 DAOOKS010000000
VERSION     DAOOKS010000606.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137065_length_1307_cov_3.623802"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="PK112_08925"
     CDS             <1..861
                     /locus_tag="PK112_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12203.1"
                     /translation="AGILAPAGGLPCLGLFPYLKRQGCPALPPEDITGLESLFGIFLY
                     CQVKQQISRLWGEHDPQVALLKRRVRDILASSEYVSWTDPSTRAVLVAPAQDAPHPRP
                     DAFVVEPDMLRRTVLAAFNRTTNRSSWCAAIFRLIRDETGAPPVVPLYELLALMVEVN
                     AAFLAGAECAPAPLPGPEELCTRAAGDRARQRAVDDTAASAIARYVTLGRITPAEGEA
                     FRKALELYLADFLAGGSDGLPAYFREVMPPEAHERYLQDYKYVFETTLNEGYRRMIDL
                     LGQSDIGLLQ"
     gene            895..>1307
                     /locus_tag="PK112_08930"
     CDS             895..>1307
                     /locus_tag="PK112_08930"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HPC12204.1"
                     /translation="MHVNVTDLLLFLDGKLSGDRRDVVQGHLAECEFCREFLEGLQDT
                     QVVGESDREPIPPPAMQLRDRIYNEALRGRVIPMTPMAPAAPPQEYLLAADGRPEERP
                     AVECLREYFSESPEMVLRVMRSAPDRRQWVQLVTD"
BASE COUNT          200 a          465 c          400 g          242 t
ORIGIN      
        1 gcgggtatct tagcgccggc cggcggcctc ccctgccttg ggctcttccc ctatctgaaa
       61 cgccagggct gcccggcgct gccgcccgag gacatcaccg gcctcgaatc cctcttcggc
      121 atcttcctct actgccaggt gaagcagcag atctcgcgcc tttggggaga acacgatccg
      181 caggtcgcgc tcctcaagcg gcgggtcagg gacatcctcg cgtcctcgga gtatgtctcc
      241 tggaccgatc cctccacccg cgccgtgctg gtggcccctg ctcaggatgc cccccatccg
      301 cggcccgatg cctttgtcgt cgagcctgac atgctgcgcc gaaccgtgct cgcggccttc
      361 aaccgcacga ccaatcgatc ctcctggtgc gccgcgattt tccgcctgat ccgcgatgag
      421 accggcgctc cgccggtcgt cccgctctat gaattgctgg ccctgatggt cgaggtgaac
      481 gccgcctttc tcgccggcgc cgagtgcgcc cccgcgcccc tccccggccc ggaagaactc
      541 tgcacccgcg ccgcgggcga tcgggcccgc caacgggcgg tggacgatac cgccgcctcg
      601 gctatcgccc gctatgtcac cctgggacgg atcactccgg ccgagggaga ggctttccgc
      661 aaggctctcg agctctacct cgccgatttc ctcgccggcg gctccgacgg cctcccggct
      721 tacttccgcg aggtgatgcc gcccgaagcc cacgagcgct acctgcagga ctacaaatat
      781 gtgttcgaaa cgactctcaa cgaggggtac cgccggatga tcgatttgct tggtcagtcc
      841 gacatcggcc tgctccagtg actattttct acgggagcat ggaaggagaa gaccgtgcac
      901 gtgaacgtga ctgatttgct cctgtttctc gacgggaagc tatccggcga ccgcagggat
      961 gtggtgcagg ggcatctcgc tgagtgcgag ttctgccggg aattccttga aggacttcag
     1021 gatacgcagg ttgtgggcga gtccgaccgg gagccgatcc ccccaccggc tatgcagttg
     1081 cgcgaccgca tatataatga ggctctgcgc ggacgggtga ttccgatgac ccccatggcg
     1141 ccggccgccc ccccgcagga gtacctcctg gccgccgatg gccgcccgga ggagcggccc
     1201 gccgtcgagt gccttcgcga gtatttctcc gaaagccccg aaatggtcct ccgcgtcatg
     1261 cgcagcgccc ccgaccggcg gcagtgggtc cagttggtga ccgatga
//
LOCUS       DAOOKS010000607         1306 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_137247_length_1306_cov_4.264588, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000607 DAOOKS010000000
VERSION     DAOOKS010000607.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137247_length_1306_cov_4.264588"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..>1306)
                     /locus_tag="PK112_08935"
     CDS             complement(15..>1306)
                     /locus_tag="PK112_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HPC12205.1"
                     /translation="PSIGLHQRDNRKLLATLTELRDIGNTVLVVEHDRETIEAADHII
                     DLGPRAGIHGGQVVAQGTLRDITRSRESLTGQYLKGERKIEIPGQRRRPNGKTIALTG
                     CRGNNLKAADVEFPLGLFIAVTGVSGSGKSTLVNETLYPILARHFFGSRRPPLPYGAI
                     AGLEHIDKVIDIDQSPIGRTPRSNPATYTGVFTHIRDLFAAIPESKARGYQPGRFSFN
                     VKGGRCEACEGDGIIKIEMHFLPDVYVPCEVCKGKRYNRETLEIAWKGRNIAEILDMT
                     VDEALAFFENIPRIKNKLLTLREVGLGYIHLGQQATTLSGGEAQRVKLAAELSKVATG
                     RTLYILDEPTTGLHFEDIRMLLGVLNELVDRGNTVIVIEHNLDVIKTADWIIDIGPEG
                     GDEGGRIISVGPPEVVARDKHSYTGVFLNAVLNGKAL"
BASE COUNT          183 a          357 c          509 g          257 t
ORIGIN      
        1 ggaggccgtc cgacttacag cgctttcccg ttcaggacgg cgttgaggaa gacgccggta
       61 taggagtgct tgtcgcgggc gacgacctcg ggggggccga cgctgatgat ccgtccgccc
      121 tcgtcgcccc cctccggtcc gatgtcgatg atccagtcgg ccgtcttgat gacgtcgagg
      181 ttgtgctcga tcacgatcac cgtgttgccg cggtcgacca gctcgttgag gacgcccagg
      241 agcatgcgga tgtcctcgaa gtggagcccg gtggtgggtt cgtcgaggat gtagagggtg
      301 cggccggtgg cgaccttgga cagttcggcg gccagtttga cccgctgggc ttcgccgccg
      361 gagagagtgg tcgcctgctg gccgaggtgg atgtagccga ggccgacctc gcggagcgtg
      421 aggagcttgt tcttgatgcg ggggatattt tcgaagaagg cgagggcctc gtcgacggtc
      481 atgtcgagga tctcggcgat gttgcgccct ttccaggcga tttcgagtgt ctcgcggttg
      541 taccgctttc ccttgcagac ctcgcagggg acgtagacgt cggggaggaa gtgcatttcg
      601 atcttgatga tgccgtcccc ctcgcaggcc tcgcaccggc cccccttgac gttgaacgag
      661 aaccgtccgg gctgataccc gcgggccttg gactcgggga tggcggcgaa gaggtcgcgg
      721 atgtgggtga acacgccggt gtaggtggcc gggttggagc ggggggtgcg gccgatcggg
      781 gactggtcga tgtcgatgac tttatcgatg tgttcgagcc cggcgatggc gccgtacggc
      841 aggggcgggc gccgagagcc gaaaaagtgc cgggcgagga tcgggtagag cgtctcgttc
      901 accagggttg atttgccgga gccggagacg ccggtgacgg cgatgaagag gccgaggggg
      961 aactcgacgt cggccgcttt caggttgttg ccgcggcagc cggtgagggc gatggttttc
     1021 ccgttggggc ggcggcgctg gccggggatt tcaatcttcc gttctccctt gaggtactgg
     1081 ccggtgagcg actcgcgcga ccgcgtgatg tcgcggaggg taccctgggc gaccacctgc
     1141 ccgccgtgaa tgccggcgcg ggggccgagg tcgatgatgt ggtcggcggc ctcgatggtc
     1201 tcgcggtcgt gctcgaccac caggacggtg ttgccgatgt cgcgcagctc ggtcagggtg
     1261 gcgaggagtt tgcggttgtc gcgctggtgc aggccgatcg agggct
//
LOCUS       DAOOKS010000608         1306 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_137265_length_1306_cov_3.291767, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000608 DAOOKS010000000
VERSION     DAOOKS010000608.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137265_length_1306_cov_3.291767"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1024)
                     /locus_tag="PK112_08940"
     CDS             complement(<1..1024)
                     /locus_tag="PK112_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12206.1"
                     /translation="MRITLFVMVLSLAIGTAAGESETDVLGRILGEVGFTRADLGYHP
                     AGYWNRFPLDVPYRLTSFDALFAEPLKLYDYATTMGNAVELYLDPTYADTASDGLYQL
                     VYNLGVDRKLGGFRSYSANLRPAPAGEEPLVDAVEGLYALAGRDTEFYTFGNRAESPD
                     MTAEIRQKTARLPDSVQVVLAGLIVNLTDAIRWRATALRNCDVADLEAALAIRDLAST
                     QGDGMVYYPELDDIAAAIDLPSMHYAALKTAAAAESAERALRRFARAVPDDFTAELPT
                     PFGTIALFSSVARSERMAPPRLFGELRASLPQWTEYDATNTLLVVDFGRRMIYQGTPG
                     AAASLGN"
     gene            1109..>1306
                     /locus_tag="PK112_08945"
     CDS             1109..>1306
                     /locus_tag="PK112_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005001518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12207.1"
                     /translation="MKGIFAVLAALVLAAGCVKEVDRSAAPKTAADTLTYATHFRGER
                     HLDRIRQLTFGGQNAEAYFNAD"
BASE COUNT          229 a          414 c          440 g          223 t
ORIGIN      
        1 ggtttcccag cgacgccgcc gcgcccggcg tcccctggta gatcatccgg cggccgaaat
       61 cgacgacgag gagcgtgttg gtcgcatcat actccgtcca ctgcgggagc gaggcgcgca
      121 gttcgccgaa gaggcggggc ggggccatcc gctcggaccg ggcgaccgaa gagaacaggg
      181 cgatcgtccc gaaaggcgtg gggagttccg cggtgaaatc gtcggggacc gcgcgggcga
      241 atcggcgcag ggcgcgctcg gcgctctcgg ctgcggccgc cgtcttcaag gcggcgtagt
      301 gcatcgaggg aagatcgatc gcggcggcga tatcgtccag ctccgggtag tacaccatcc
      361 cgtccccctg cgtgctcgcc aggtcgcgga tcgccagcgc agcctcgagg tccgcaacgt
      421 cgcagttgcg cagcgccgtc gcgcgccagc gaatcgcatc cgtgagattc acaatcagcc
      481 ccgcgaggac aacctgcacc gagtccggga gacgcgcggt tttctgtcgg atctccgcgg
      541 tcatgtcggg cgattcggcg cggttgccga aggtgtagaa ctcggtgtcg cggccggcca
      601 gcgcgtagag cccctccacc gcgtcgacca gcggctcttc ccccgccggg gccggccgca
      661 ggttcgccga gtaggagcgg aagcccccca gttttctgtc gacccccagg ttgtacacca
      721 actggtagag accgtcggaa gccgtgtcgg cgtaggttgg atcaaggtac agctcgacgg
      781 cgttccccat ggttgtggcg tagtcgtaca gcttcagcgg ttcggcaaag agcgcatcga
      841 acgaggtcag ccggtaggga acgtcaagcg ggaagcggtt ccagtagccc gcggggtggt
      901 agccgagatc cgcgcgcgtg aatcccactt cgccgaggat tcggccgagg acgtcggttt
      961 cggactcccc cgcggcggtt ccgatcgcca gtgacaggac catgacaaag agcgtaattc
     1021 tcattgtact ttggctcccg gttgattttt cttagacata tatgcagcat aacctcttca
     1081 ttggcaacag catttgatgg aggaccgggt gaaaggaatc ttcgcagtgc tcgcggctct
     1141 cgttttggcg gcggggtgtg tgaaagaggt ggaccggagc gccgctccga aaacggcggc
     1201 cgacacgctc acctacgcca cccacttccg gggcgagcgg catctcgacc gcatccgcca
     1261 gctcactttc ggcggccaga acgccgaagc ctatttcaac gccgac
//
LOCUS       DAOOKS010000609         1305 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_137464_length_1305_cov_2.676800, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000609 DAOOKS010000000
VERSION     DAOOKS010000609.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137464_length_1305_cov_2.676800"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="PK112_08950"
     CDS             complement(<1..533)
                     /locus_tag="PK112_08950"
                     /inference="COORDINATES: protein motif:HMM:NF018460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HPC12208.1"
                     /translation="MNTLILIWIGAVGLAIGSFLNVVIYRLPLKKRFGLGRSICPKCG
                     TQLRWYHNIPLASWAALRGKCAFCRQPISWRYPLVELMTAGAYVYFWAYLGFSPMFFV
                     WAALVSAVIVIIFVDLDHQIIPDSITLSGMAAGLAASFLPNGLGIVQSLIGLVVGGGS
                     LYLIAVLGVHADDHTVDG"
     gene            complement(561..>1305)
                     /locus_tag="PK112_08955"
     CDS             complement(561..>1305)
                     /locus_tag="PK112_08955"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HPC12209.1"
                     /translation="PFFYAPPEDLSGDAVVLRGDEAHHALRVLRLGIGDRLLVIDGLG
                     AARRGEIVEAGARGATVTVRVRDTIRNFGEPAVRLTLAAGLSAGAKFDAVVQRGTELG
                     VQRFVPILGEKSKVRMEDAGRAAARVRRLEKVALAAVKQCRRSYRPEIALPTGLESFL
                     AYTDPEALNLVFHAAPGGKPLSAQAIPPGVSRVSLLVGPEAGFSEAEAGRAIAAGYAP
                     ISLGPRILRSETAGPVVCALVMHALGELS"
BASE COUNT          243 a          483 c          408 g          171 t
ORIGIN      
        1 ccatcgacgg tgtgatcgtc ggcatgtacc cccagcaccg cgatcaggta gagcgatcct
       61 ccacccacga cgagaccgat gagtgactgc acgatgccga gcccgttcgg tagaaacgac
      121 gccgccagcc ccgccgccat gcccgacagc gtgatcgaat cgggaatgat ctggtggtcg
      181 agatccacga agatgatcac gatgacggcc gagacgagtg ccgcccagac aaagaacatg
      241 gggctgaacc cgaggtaggc ccagaagtac acgtacgccc cggccgtcat cagctccacc
      301 agcgggtacc gccacgaaat cggctgccgg cagaatgcac acttcccccg cagagcggcc
      361 cagctggcca gcggaatgtt gtggtaccac cgcagctgcg tcccgcactt cggacagatc
      421 gaccgcccga ggccgaaccg cttcttcagc ggcagccggt agatgacgac gttgagaaac
      481 gagccgatcg ccaacccgac cgcgccgatc cagatgagaa tgagtgtatt catacactct
      541 ctgtatcggc ggatcgggcg ctaactcaac tcgccgagcg cgtgcatgac cagcgcgcag
      601 accaccggac cggctgtctc gctccgaagg atgcgcgggc cgagactgat cggcgcgtag
      661 ccggccgcga tcgcccgccc cgcctcggcc tcggaaaacc ccgcctccgg acccaccagc
      721 agcgagaccc ttgacacgcc cggcgggatc gcctgggccg agagcggctt tccccccggc
      781 gcggcgtgaa acacgaggtt cagggcctcg gggtcggtgt acgcgaggaa cgactcgagg
      841 ccggtcggca gggcaatctc cgggcgatag gaacggcggc attgcttgac ggccgccagg
      901 gccaccttct ccaggcggcg cacccgggcc gccgcccgcc cggcgtcctc catcctgacc
      961 ttggatttct ccccgagaat cggcacgaac cgctgcacgc ccagctcggt tcctctctgg
     1021 accacggcgt cgaacttcgc ccccgccgac agcccggccg ccagcgtcag ccgcacggcc
     1081 ggctccccga aattgcggat cgtgtcgcgg actcgcacgg tcaccgtcgc cccccgcgct
     1141 cccgcctcga cgatctcccc ccgccgcgcg gcgccgagac cgtcgatcac cagcaggcgg
     1201 tccccgatgc ccaggcgcag gacgcgcagg gcatggtgcg cctcatcccc ccggagcaca
     1261 accgcgtctc cggagaggtc ctcaggggga gcgtagaaga agggg
//
LOCUS       DAOOKS010000610         1293 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_139610_length_1293_cov_2.100162, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000610 DAOOKS010000000
VERSION     DAOOKS010000610.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139610_length_1293_cov_2.100162"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="PK112_08960"
     CDS             complement(<1..283)
                     /locus_tag="PK112_08960"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HPC12210.1"
                     /translation="MLFSKACSYAIRAAVLAAAKDPDGRRAFIPIRELADEVGVSFHF
                     LTKILRKLTEVEIMESFRGPNGGVGLVRPARRSLLSTSSTGRPMIRAVNS"
     gene            complement(636..1046)
                     /locus_tag="PK112_08965"
     CDS             complement(636..1046)
                     /locus_tag="PK112_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12211.1"
                     /translation="MNKRTGLLLTLALTALALLSCGGSRTLVLYEGERRPAEQVAVLD
                     VSHKSLYVMGIDTLVHRYRALSEEHIEVLPGEHTLVLNYSSWKGTSDEPMTLSFEAQA
                     GHLYQVKASAGYRRWTAWIIDMNDGSVVAGSPPD"
BASE COUNT          239 a          418 c          360 g          276 t
ORIGIN      
        1 ccgagttgac cgcgcggatc atcggccgcc cggtcgagct ggtggacaac aacgaccgcc
       61 tggccggccg caccaagccc acccctccgt tcggtccgcg aaaggactcc atgatctcga
      121 cctccgtcaa cttgcggagg atcttggtga gaaagtggaa cgagacgccg acctcatcgg
      181 ccagctcgcg gatcgggata aacgcccgcc gcccgtcggg atctttggcc gcggcgagca
      241 cggcggcgcg gatcgcgtag ctgcacgctt tggaaaacag catcttttcg gttcacattc
      301 cagttttagg actcttttat ctgaaatatt atatcgccgt tcatttctgt caagcccatt
      361 ttgctgtttc acggcacaaa attcgcccgg tggacgcctc tgtcgtttgc gtccctgccc
      421 ggcatccttg tgcgcaggat gttctcggca gccaacaact cttacatcaa tgacttatgg
      481 tgtacttgca ggagtagccg atagtcccct caaatctgcc tgcctctttc ccagcctcgc
      541 cgcgcgcttc attatatgtc gtcagcttcc gccacccgcc gagtcaaacc cctccttcgg
      601 tcgttccagg ccgggcgccg ctacggtacc gcacgtcaat cgggcggcga gccggccacc
      661 accgacccgt cgttcatatc gataatccac gccgtccacc gccggtaacc cgctgatgct
      721 ttgacctggt agagatgtcc ggcctgcgcc tcaaacgaca gcgtcatggg ctcatcggag
      781 gtccccttcc acgaggagta gttcaggacc agcgtgtgtt ctcccggaag tacctcgatg
      841 tgctcttccg agagcgctcg gtagcggtgg accagcgtgt cgatccccat cacgtacagc
      901 gacttgtgac tgacatcgag aaccgcgacc tgctccgccg gccgacgctc gccctcgtag
      961 agcaccagcg tccggctccc tccgcacgag aggagagcga gtgcggtcag cgccaaagtt
     1021 aaaagcagtc ccgttcgctt gttcatggcc gatttcctca tgcctcatgg tcgtcgctgt
     1081 tcgggtggga agcaagcgga agttttgcca gacacggttg attctgaccg tgcacaatgt
     1141 gacactcacc ggccgattgg tgatttactt tctccgccgc ttgaccgtat tcattggggc
     1201 gggacacaat cgggaggaaa gcgcgagggc cggcgccccc ctttcgtggc gaccgactcg
     1261 ggggataacc gggatctcgt gaagattgat caa
//
LOCUS       DAOOKS010000611         1291 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_139902_length_1291_cov_2.635113, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000611 DAOOKS010000000
VERSION     DAOOKS010000611.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139902_length_1291_cov_2.635113"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="PK112_08970"
     CDS             <1..285
                     /locus_tag="PK112_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013129558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12212.1"
                     /translation="MDTMEMIHDAINLKVAYVVGTAFYTDGSGRNEMRLNYSYPTEEQ
                     IELGVERLGKLIRMRVRGEKRAGKTPADDTDEEECAACADTGDRTSEQDR"
     gene            complement(370..>1291)
                     /locus_tag="PK112_08975"
     CDS             complement(370..>1291)
                     /locus_tag="PK112_08975"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HPC12213.1"
                     /translation="GEAAFALMHQLLDRVWGEVARVLAPGGIACVNIGDAARSIGSGF
                     RLFPNHARVIDAFLRLGLHNLPNIIWRKPTNAPNKFMGSGMLPPGAYVTLEHEYILMF
                     RKGDKRVFRSPDEQRRRAESAIFWEERNRWYSDVWSDLTGAPQKLASAARSRSGAFPF
                     ELAWRLVNMFSIKGDVVLDPFVGTGTTLLAAAANERHSIGYEIEFGLRPALLRQLGET
                     PGVSRQVLRERIERHLALVGRRTAEGKPPTFTVSRHGWPCVSRQETGMVLRPAASVRQ
                     FSEETTALSVQWEVSYGEIGPGEQVEMPLA"
BASE COUNT          226 a          453 c          414 g          198 t
ORIGIN      
        1 atggacacca tggaaatgat ccacgatgcg atcaacctga aagtcgccta cgtggtcggc
       61 acagcgttct acaccgacgg ctccggccgc aacgagatgc ggctcaacta ctcctacccg
      121 accgaagagc agatcgaact gggcgtcgaa cgtctcggca aactcatccg catgcgcgtg
      181 cgcggcgaaa agcgcgcggg aaagacgcct gccgacgaca ccgacgaaga agagtgtgcg
      241 gcttgcgctg acaccggcga ccgcacatcg gagcaggata ggtagggacc atccgccggt
      301 gatatcggcc cgctcacgca aagtggcggg ccgcccgtcg gtcgaggcgc ccgacgccgc
      361 gccgtcggcc taggcgagcg gcatctccac ctgctccccc ggtccgatct cgccgtagct
      421 tacctcccac tgcacggaga gcgcggtcgt ctcctcgctg aactgccgca cgctcgccgc
      481 cggccggagc accatccccg tctcctggcg gctcacgcac ggccacccgt ggcggctcac
      541 tgtgaacgtc gggggcttgc cctcggccgt ccgccggccg accagggcca ggtggcgctc
      601 gatccgttct ctcaacacct gtcgcgagac ccccggcgtt tccccgagct ggcgcagcag
      661 cgccgggcgc agaccgaact cgatttcgta gccgatgctg tggcgttcgt tcgccgcggc
      721 ggccaggagg gttgtcccgg tcccgacgaa cgggtcgagc acgacatccc ccttgatcga
      781 gaacatgttg accagccgcc aggcgagttc gaacggaaag gcgccgctgc gcgagcgcgc
      841 cgccgaggcc agtttctgcg gcgccccggt gagatccgac cagacgtcgg aataccaccg
      901 gttgcgctcc tcccagaaaa tggcgctctc cgcccgccgt ctctgctcgt caggactgcg
      961 gaacacccgc ttgtccccct tgcggaacat gaggatgtac tcgtgctcga gcgtcacgta
     1021 ggctcccggc gggagcatcc ccgagcccat gaatttgttc ggtgcattgg tcggcttgcg
     1081 ccagataatg ttggggaggt tgtgcagccc gagccggagg aaggcatcga tcacccgggc
     1141 atggttggga aagaggcgga acccggaccc gatactgcgg gcggcatcgc cgatattgac
     1201 gcacgcgatt ccccccggcg cgagcacgcg ggcgacttcc ccccagacgc ggtccagcag
     1261 ttggtgcatg agggcaaagg ccgcctcccc t
//
LOCUS       DAOOKS010000612         1288 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_140379_length_1288_cov_3.198702, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000612 DAOOKS010000000
VERSION     DAOOKS010000612.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140379_length_1288_cov_3.198702"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="PK112_08980"
     CDS             <1..376
                     /locus_tag="PK112_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12214.1"
                     /translation="AGELHGAEVPIKKYFITAEIVTGEGEIIQPGSRCFKSVSGYDIV
                     RIFAGAWGLLGLIVRATFRVMPIAGAGDFATMRQRAITRSPLAAALDRDNDAADAVYS
                     RKIKDKFDPRGVLPAVERAGSL"
     gene            complement(394..1023)
                     /locus_tag="PK112_08985"
     CDS             complement(394..1023)
                     /locus_tag="PK112_08985"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HPC12215.1"
                     /translation="MRPLLPILLCALTLAVPIGAWSDDAPADSVAAPSDTVAPAPPRD
                     TVRYLRTSDLDRFLPSTDSVDLERRLSQNPMVALFKSMLVPGWGQFGNGAYVKAILFF
                     GLDAWLVSSAIDHGRKASDYFDSYQSAADVTDRNLWYDRYDDERSRRNKYTWFAVIVS
                     FVSMFDAYVDAHFSGFPDADRARRSEPGLSLNLEPAPEGGVTATLSLTF"
     gene            complement(1031..>1288)
                     /locus_tag="PK112_08990"
     CDS             complement(1031..>1288)
                     /locus_tag="PK112_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12216.1"
                     /translation="ASLSDDGEMEIIFDARCSDRDLGRYIRDVYKGKYVYGSAVRLYR
                     GAYLNFNPGKAQPALLDGDLVELPAQEYNFRISPTRLNLFC"
BASE COUNT          246 a          365 c          470 g          207 t
ORIGIN      
        1 ggcgggggaa ctgcacggag cggaagtgcc gatcaagaag tattttatca cggcggagat
       61 agtcaccggc gagggggaga tcatccagcc ggggtcgcgc tgtttcaagt cggtgtcggg
      121 gtacgacatt gtccggattt tcgccggcgc ctggggactg ctggggctga tcgtgcgggc
      181 gacgttccgc gtgatgccga tcgccggggc gggagatttc gcaaccatgc gtcagcgggc
      241 gatcacgcgc tcgccgctgg cggccgccct cgaccgggac aacgacgccg ccgacgccgt
      301 gtactcgcgc aaaatcaagg acaagtttga cccgcgcggc gtgctgcccg cggtcgagcg
      361 cgccggctcg ctgtgagctg cggcggaaga gagtcagaaa gtcagcgaga gtgtggcggt
      421 gacgccgcct tcgggggcgg gctccaggtt gaggctcaga cccggttcgc ttcggcgggc
      481 gcggtcggca tcgggaaatc ccgagaagtg ggcatcgaca tacgcatcaa acatggacac
      541 gaacgagaca atcaccgcaa accacgtata cttgttgcgc cggctacgct cgtcatcgta
      601 acggtcatac cagaggttgc ggtcggtgac gtccgcggcc gattggtagc tgtcgaagta
      661 gtcggaggcc ttccgcccgt ggtcgatggc cgagctgacc agccaggcgt ccaggccgaa
      721 gaaaagaatc gctttgacat aggcgccgtt gccgaactgg ccccaccccg gaaccagcat
      781 cgatttgaac agggcgacca tcgggttctg cgagaggcgc cgttcgaggt cgaccgagtc
      841 ggtggagggg aggaagcggt cgaggtcaga cgtgcgcagg tagcgaacgg tgtcgcgcgg
      901 cggggcgggg gccaccgtgt cggacggggc ggcgacggag tccgcgggag cgtcatccga
      961 ccaggcgccg ataggaacgg ccaaggtgag ggcacacaga aggatgggga ggaggggcct
     1021 catggacgac tcagcagaag aggttcagtc tggtcgggct gatccggaag ttgtactcct
     1081 gcgcggggag ttccaccaga tcgccgtcga gcagggccgg ctgcgccttg ccggggttaa
     1141 aattgagata ggcgccccgg tagagccgca cggcgctgcc gtacacatat ttccctttat
     1201 acacgtcgcg gatgtagcgc ccgagatctc tgtccgagca gcgggcgtcg aaaatgatct
     1261 ccatctcgcc atcatcggaa agcgaggc
//
LOCUS       DAOOKS010000613         1288 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_140428_length_1288_cov_2.477697, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000613 DAOOKS010000000
VERSION     DAOOKS010000613.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140428_length_1288_cov_2.477697"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="PK112_08995"
     CDS             <1..810
                     /locus_tag="PK112_08995"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF017864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HPC12217.1"
                     /translation="EPRQLAIMCGSHNGSDAHREAVKGNLERAGFTAADLQCGAHWPI
                     GMEQARVFPAHGEERDPLRHNCSGKHSGFLALAKFLGDPPERYLDPAAKGQALVRRAL
                     AEVCEYPEAQMPAGIDGCSAPNYPLPLYNLALGSKKLAATEAAEEGLRGALRRVRDAM
                     LAHPEMVSGEGRIDLDLARSFPGRMVCKIGAEGIEGIGLVDPPLGIAIKIADGSWRAM
                     GAICVAIFRQLGLIDDIMRFPLLGPHENPAVRNARGIVTGRIAAEVVLKRA"
     gene            951..>1288
                     /locus_tag="PK112_09000"
     CDS             951..>1288
                     /locus_tag="PK112_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HPC12218.1"
                     /translation="MAKDHPPVEPFRIKSVEPVRLLSRPERERRVVEAHYNVFKIDAA
                     DIYIDLLTDSGTAAMSNRQWAALMMGDESYAGATSFRRFERAVREIFGKRFVIPCHQG
                     RAAENLMFSTV"
BASE COUNT          208 a          376 c          501 g          203 t
ORIGIN      
        1 gagccgcgcc agctggcgat catgtgcggg tcccacaacg ggagcgacgc gcaccgggaa
       61 gcggtgaagg gcaacctgga gcgggcgggg ttcacggcgg ccgatctgca gtgtggcgcg
      121 cactggccga tcgggatgga acaggcgcgg gtgttcccgg cgcacgggga ggagcgcgac
      181 ccgctgcgcc acaattgttc ggggaagcac tccggctttc tcgcgctggc gaaatttctg
      241 ggcgatcctc cggagcgcta cctcgacccg gcggcgaagg gacaggcgct ggtgcgccgg
      301 gcgctcgccg aggtgtgcga gtatcccgag gcgcagatgc cggccgggat cgacgggtgc
      361 agcgcgccga actacccgct gccgctgtac aacctggcgc tgggatccaa gaagctggcg
      421 gcgacggagg cggcggagga ggggctgcgg ggggcgctgc ggcgggtgcg ggacgccatg
      481 ctggcccacc ccgagatggt gtcgggggag gggcggatcg acctcgacct ggcgcgctcg
      541 tttccggggc ggatggtgtg caagatcggg gcggagggaa tcgaggggat cggcctggtc
      601 gacccgccgc tggggattgc gatcaagatt gccgacggga gctggcgggc gatgggcgcc
      661 atttgtgttg cgattttccg gcagttgggg ttaattgacg atatcatgcg gtttccgctc
      721 ttagggccgc acgagaaccc cgcggtgcgc aacgcgcgcg ggattgtcac cgggcggatt
      781 gccgcggagg tcgttttgaa gcgagcgtag gcacgtatgc gcggattcgg ctatcgggtt
      841 ccccacctgg gacgggacta ccaccagcgg tgggtcaagc gggcgaccta acggcgacca
      901 tgtcaccgga tcgttcacgg cagttttcac ccagaacaaa ggagaaggcc atggccaagg
      961 accatccgcc ggtcgagccg ttccgcatca agtcggtgga gccggtgcgg ctgctgagcc
     1021 ggccggagcg cgagcgcaga gtcgtcgagg cccactacaa cgttttcaag atcgacgcgg
     1081 ccgacatcta catcgacctc ctgaccgatt cggggacggc ggcgatgtcg aaccgccagt
     1141 gggcggcgct catgatgggc gacgagtcgt acgcgggggc gacctcgttc cgccggttcg
     1201 agcgggcggt gcgggagatt ttcggcaagc ggtttgttat cccctgccac caggggcggg
     1261 ccgcggagaa cctgatgttc tccacggt
//
LOCUS       DAOOKS010000614         1273 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_143005_length_1273_cov_3.263547, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000614 DAOOKS010000000
VERSION     DAOOKS010000614.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143005_length_1273_cov_3.263547"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            160..768
                     /gene="purN"
                     /locus_tag="PK112_09005"
     CDS             160..768
                     /gene="purN"
                     /locus_tag="PK112_09005"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012475272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HPC12219.1"
                     /translation="MADGRRPRIGVFLSGGGSNLQALIDASKCGILSGEIALVVSNLR
                     KAFGLERARQVQIPVFVYKEADYATPEEGQAALLRVLKEHGVEWIALAGYLKLLPGNI
                     LRAYPRRVVNIHPALLPKYGGKGMYGHFVHEAVLAAGEKESGLTIHLVDEAYDHGAVL
                     HQQRVPILPGDTPETLAARVLEQEHRWYARVLDKLIRGEYGG"
     gene            758..>1273
                     /locus_tag="PK112_09010"
     CDS             758..>1273
                     /locus_tag="PK112_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HPC12220.1"
                     /translation="MAAETDRVVMQTEIREYPLMRRGKVRDIYDLGDTLLFVATDRIS
                     AFDVVMPNGIPGKGRVLTQMSLFWFDFLSDVVESHLVTADVDRYPAALRAYREQLEGR
                     SMIVAKADRVEAECIVRGYISGSMWKELQEARKRGTNAVHGFEFPPALGESEQLPRPI
                     FTPSTKNDDGHD"
BASE COUNT          235 a          362 c          476 g          200 t
ORIGIN      
        1 atggtgaaca tggagatacc ggagcagttt gatggggata cgggggcgtt tctgcgggcg
       61 gcagagaagg tttgactttg gccggcgaag gcgtgtactt agcaccttgg cggaccggcc
      121 ggtgaaggca aggcggcaag cgagggacaa acggacagga tggcagacgg cagacggccc
      181 cggatcggcg tatttctctc cggcgggggc agcaacctcc aggcgctgat cgatgcctcg
      241 aagtgcggga tcctctcggg agagatcgcg ctggtggtgt caaacctgcg caaggcgttc
      301 gggctggagc gggcccggca ggtccagatc cccgtgttcg tctacaagga ggcggactac
      361 gcaaccccgg aggaggggca ggcggcgctc ctgcgcgtgc tgaaggagca cggcgtggag
      421 tggatcgcgc tcgccggcta cctgaaactc cttccgggaa atatcctccg cgcatacccg
      481 cggcgggtcg tgaacatcca cccggcgctg ctgccgaaat acgggggcaa ggggatgtac
      541 ggacatttcg tgcacgaggc ggtgctggcg gccggggaga aggaatcggg gctgacgatc
      601 cacctggttg acgaggcgta cgaccacggc gcggtgctcc accaacagcg ggtgccgatc
      661 ctgccggggg acacgccgga gacgctggcg gcgcgcgtgc tggaacagga acatcgctgg
      721 tacgcccggg tgctggacaa actcattcga ggtgaatatg gcggctgaga cggatcgggt
      781 ggtgatgcag accgagatcc gcgagtaccc cctgatgcgg cgggggaagg tgcgggacat
      841 ttacgatctc ggcgacacgc tgttgttcgt ggcgaccgac cgcatttcgg cgttcgacgt
      901 ggtcatgccg aacggcatcc ccggcaaggg gcgggtactc acgcagatgt cgctgttctg
      961 gttcgacttc ctcagcgatg tggtcgagag ccacctggtc acggccgacg tcgaccggta
     1021 cccggcggcg ctgcgcgcct accgggagca gctcgagggg cgctcgatga tcgtggccaa
     1081 agccgaccgg gtggaggccg agtgcatcgt gcgggggtac atttccgggt cgatgtggaa
     1141 ggaactgcag gaggcgcgaa agcggggaac caacgcggtg cacggatttg agttcccgcc
     1201 ggctctgggc gagtcggaac agctcccccg cccgatcttc acgccctcga ccaagaacga
     1261 cgacgggcac gac
//
LOCUS       DAOOKS010000615         1265 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_144460_length_1265_cov_2.938017, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000615 DAOOKS010000000
VERSION     DAOOKS010000615.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144460_length_1265_cov_2.938017"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            31..618
                     /locus_tag="PK112_09015"
     CDS             31..618
                     /locus_tag="PK112_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12221.1"
                     /translation="MRMVARQTVVRVAVAALVGILAYAGIAGAAVRKPAKTSVPVPQV
                     VTTPSQSAPVPSAAAEPTLRQEPAEAQVNWQVMAGGGGSGTAGALQFSATIGQPIVGW
                     TSATGVNVHAGFWQDFSSGQCCENRGNVDHKFSGMNPVSVADITYLIAYVFRGGPVPP
                     CPEEADLTADGDITVVDITYLIAYVFRGEPAPPAC"
     gene            862..>1265
                     /locus_tag="PK112_09020"
     CDS             862..>1265
                     /locus_tag="PK112_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase"
                     /protein_id="HPC12222.1"
                     /translation="MKVEEVINSSFLKPGSVIYSAGNAATPQVILGQLARDPAIRDID
                     LYCVLPLGDRIAALYTEERCQGLTHRVIFNSRLTKEAVNNGWAKYHPMHLREIPRTMM
                     NVVKPNIVLCSVAGPDNGGYYSLGTTVEGTLGA"
BASE COUNT          231 a          360 c          454 g          220 t
ORIGIN      
        1 cgtgacagat cgctgataga ggagtgcgca atgagaatgg tcgctcgtca aacggtggtg
       61 agggttgcgg ttgcggcgct ggtcggcatt ctggcctacg ccggcatcgc gggggccgcg
      121 gtgcggaaac cggcgaagac cagtgtgccg gtgcctcagg ttgtcacgac gccgagtcag
      181 agcgctccgg tgccgagtgc ggcggcggag ccgaccttgc ggcaggagcc cgcggaggct
      241 caggtcaact ggcaggtgat ggcaggcggg ggcggcagcg gcacggccgg agcgctgcag
      301 ttctcggcca cgatcggcca gccgatcgta ggctggacca gcgccaccgg ggttaatgtc
      361 cacgccgggt tctggcagga tttcagttcc ggtcagtgct gtgagaaccg ggggaatgtc
      421 gaccacaaat tcagcggtat gaatccggtc tcggtggcgg acatcactta cctgatcgcc
      481 tacgtcttcc gcggcggacc ggtgccgccg tgcccggagg aagcggattt gacggccgac
      541 ggcgacatca cggtggttga catcacgtat ctgattgcgt acgtcttccg cggcgaaccg
      601 gcgccgccgg cctgctgagc ggcgcagggc gggcgggttc gactgcgaca gggtggccac
      661 ggtcgggaca aagcagcccg gcggcgaccc atcgagcgag agcgcgctct cgttccgacc
      721 ttattttcgc cgacagatag aaaagggtgg cggcccgggc aggcttgtgt tatttttcac
      781 aagcccgccg ccggtatgcc ccggggccgg gattaagtat gaacgtgcag aagaaggtgt
      841 tggcaggtct gtatgcgaat cgtgaaagtt gaagaagtca tcaactccag ttttctcaaa
      901 cccggaagcg tgatctactc agccggcaac gcggccacgc cgcaggttat cctcgggcag
      961 ttggcccgcg atccggcgat ccgggatatc gatctctact gcgtgcttcc cctgggcgac
     1021 cggatcgcgg cgctctacac ggaggaacga tgccaggggt tgacgcaccg ggtgattttc
     1081 aacagccggc tgaccaagga ggcggtcaat aacggctggg cgaagtatca tccgatgcac
     1141 ctgcgggaga ttccgcgcac catgatgaac gtcgtgaagc cgaacatcgt gctctgctcg
     1201 gtggcggggc cggacaacgg cggctactac agtctgggga caaccgtgga ggggacgctg
     1261 ggggc
//
LOCUS       DAOOKS010000616         1265 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_144491_length_1265_cov_2.426446, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000616 DAOOKS010000000
VERSION     DAOOKS010000616.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144491_length_1265_cov_2.426446"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="PK112_09025"
     CDS             complement(<1..204)
                     /locus_tag="PK112_09025"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481980.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HPC12223.1"
                     /translation="MAENIGRVVQVIGPTVDCEFRSDALPNILNAIRIVDQARNIDLT
                     VEASMHVGDKIVRCVALASTDGLV"
     gene            complement(261..1127)
                     /gene="atpG"
                     /locus_tag="PK112_09030"
     CDS             complement(261..1127)
                     /gene="atpG"
                     /locus_tag="PK112_09030"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HPC12224.1"
                     /translation="MPTLRDVKKRIRSVRSTRRITGAMEMVAAAKLRRAQQKVEQTRP
                     YADKLEEMLAHLAAGSSNEIVHPYFEERPVRKRTLVLFTGDRGFCGSYNANIIRDTLA
                     WLRDHTAVATELVVIGKKGYDYFRHRPQAVVGAHPGFNTTLDYGKARDIVGFLTHRFV
                     SGETDRIDLLYTRFLSLSKYRITLQSYLPIRPPAVGAAAPGGADYIFEPDAEHIYAAL
                     MPSYATTMFVTALVESFASELGSRMIAMNAATKNAGEMIDRLTLDYNKARQAQITKEL
                     LEVVSGAEALKG"
BASE COUNT          191 a          422 c          398 g          254 t
ORIGIN      
        1 caccaggccg tcggtcgagg ccagcgccac gcaccggacg attttgtcgc cgacgtgcat
       61 ggaggcttcg acagtcagat cgatatttct cgcctgatcg acaattcgga tggcgttgag
      121 gatgttcggg agcgcgtccg agcggaactc gcagtccacc gtcggcccga ttacctgcac
      181 caccctgcca atgttttcag ccattgagat aactccgcat ctatgtcaca tcattctctt
      241 cgcgtgaatc cgccccggcc tcatcctttc agcgcctccg cccccgagac cacttcgagc
      301 agttctttgg tgatctgggc ctgccgcgcc ttgttgtagt cgagggtgag ccggtcgatc
      361 atctccccgg cgtttttcgt cgccgcgttc atggcgatca tccggctgcc cagctcgctc
      421 gcgaacgact ccacgagggc ggtgacgaac atcgtcgtcg cgtagctcgg catgagcgcc
      481 gcgtagatgt gctcggcatc cggctcgaag atgtagtcgg cgccgcccgg cgccgccgcc
      541 ccgaccgccg gcggccggat ggggagatag ctctgcagcg tgatccggta cttgctgagc
      601 gagagaaacc gggtgtagag caggtcaatg cggtcggtct cgccgctcac aaagcggtgg
      661 gtgaggaagc cgacaatgtc gcgcgccttg ccgtaatcga gcgtcgtgtt gaaaccgggg
      721 tgcgccccga ccaccgcctg cgggcggtgg cggaaatagt cgtacccctt cttgccgatg
      781 acgaccagtt cggtcgcgac cgccgtgtgg tcgcggagcc aggcgagcgt gtcgcggatg
      841 atgttggcgt tgtaggagcc gcagaagccg cggtcgcccg tgaaaagcac gagcgtcctc
      901 ttcctgaccg ggcgctcctc gaaatacggg tggacaatct cgttactcga tcccgccgcc
      961 aggtgggcca gcatctcttc caacttgtcg gcgtacggcc gggtctgttc gaccttctgc
     1021 tgggcccgcc ggagcttcgc cgcggccacc atctccatcg cccccgtgat gcgccgggtc
     1081 gagcggacgg agcggattct cttcttgacg tcgcgcagtg tgggcatgcg tcgctacacc
     1141 tgtttgtgcg gtcccggtcc ggccgccgcc ggggcggcgc ccggcgccga ggctgcaaac
     1201 tgcgccttga acttcgccac cgccgcctgc agctctttct ccgtcgactc cgaaatcttc
     1261 ttctc
//
LOCUS       DAOOKS010000617         1261 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_145177_length_1261_cov_2.869818, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000617 DAOOKS010000000
VERSION     DAOOKS010000617.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145177_length_1261_cov_2.869818"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..>1261)
                     /locus_tag="PK112_09035"
     CDS             complement(102..>1261)
                     /locus_tag="PK112_09035"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPC12225.1"
                     /translation="EGGMFVQFIHTYQMDWPTFAMVGRTFADVFPNALLVATSPDTAS
                     NDLMLVGIKGSGPLNLARAREMGQYCRQGGNFAVRDPAVLVRLVRAQNLKELFGGGPL
                     NTDSHPRLEYAAPKLLYRSDPEIAARIAAGGRLTEPLARLKEKVATEVESQIDFLEYE
                     LSVHLPMHGFVPPILAYDRATPAQRARHDSLLCAYCRQNPVVLSLCQDERARESCAAC
                     QIEGLEATVAAGRATAMTYSYLAFLYSRGGQTDKLPALYREWVQSDPREAKAYHGLAA
                     ALIERNNLTEALPALLTADRENPGNPVILTDLGYLYGRMGRPDLARDYFGRALQADIR
                     YARAHMGMGAAEATAGNIPAAIAHYREALRLDPALEGAQTALAKLQAGRQP"
BASE COUNT          184 a          444 c          421 g          212 t
ORIGIN      
        1 acccggagct ctttctcgcc gaacttcagg ctgcggcgcg cgaggcctag ttcgttccgc
       61 gcttgcggtg ttgaaacggg tggtgcgggc tgggccgcgg gtcagggctg ccggcccgcc
      121 tggagcttgg ccagcgctgt ctgagccccc tccagggccg ggtcgaggcg aagggcctcg
      181 cggtagtgtg cgatagcagc cgggatgttg cccgcggtcg cctcggccgc ccccatcccc
      241 atgtgcgccc gggcgtaccg gatgtccgcc tgcagcgctc ggccgaaata gtcccgcgcc
      301 aggtcgggcc ggcccatccg gccgtagagg tagcccaaat cggtcagaat gaccggattg
      361 cccggattct cccgatccgc cgtcagcagc gcgggcaagg cctcggtcag gttgttgcgt
      421 tcgatcagcg ccgcggccag accgtggtag gccttcgcct cgcgaggatc gctctgcacc
      481 cactcccggt acagcgcggg cagtttgtcc gtctggccgc cccggctgta gagaaaagcc
      541 aggtagctgt aggtcatggc ggtcgcccgg ccggccgcca cggtcgcctc aagcccctcg
      601 atctggcagg cggcgcacga ttcccgcgcc cgctcgtcct ggcagagcga gagcaccacc
      661 gggttttgcc ggcagtatgc gcacaggagg ctgtcgtgcc gcgcccgctg cgctggggtc
      721 gcccggtcgt atgcgagaat gggcggcaca aaaccgtgca tcggcagatg taccgacaat
      781 tcgtattcca gaaaatcgat ctgcgactcg acctcggtcg ccactttctc cttcagccgc
      841 gcgagcggct ccgtcagtct ccccccggcg gcgatccggg ccgcgatttc ggggtccgac
      901 cggtacagca gcttgggcgc cgcatactcc aggcgcgggt ggctgtcggt gtttagcggg
      961 ccgccgccga acaactcttt caggttctgc gcccggacca gccggacgag cacggcggga
     1021 tcgcggaccg cgaagttgcc gccctgccgg cagtactgtc ccatctcccg cgcgcgggca
     1081 agattcagcg gcccgctccc cttgatcccg acaagcatca gatcattgct ggccgtgtcc
     1141 ggcgaggtcg ccaccaggag cgcgttcggg aaaacgtcgg cgaacgtccg ccccaccatc
     1201 gcgaacgtcg gccagtccat ctggtacgtg tggatgaact ggacgaacat ccccccctcc
     1261 g
//
LOCUS       DAOOKS010000618         1253 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_146685_length_1253_cov_2.679466, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000618 DAOOKS010000000
VERSION     DAOOKS010000618.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146685_length_1253_cov_2.679466"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..758
                     /locus_tag="PK112_09040"
     CDS             <1..758
                     /locus_tag="PK112_09040"
                     /inference="COORDINATES: protein motif:HMM:NF015039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HPC12226.1"
                     /translation="DVAQLRARKERMLAEIYRLLVAAYGPPPTEFTFRYKVKDSVVTE
                     PKTYTPRSFYDEIFGGPLPDYAILMDNPAKGYDTLYRIESSRNMADRADLTMLNLPVA
                     RLKEYCLRALLDSQAVWFACDVGVDNFGDSGILRNGIYDFEGTLGVSFAIAKADRITL
                     RDSYPNHAMTLVGVDTAADGSPVKWLVANSWGDKGGNDGYWIMYNDWFDTNVYLVAVE
                     KRLLAEADRKKLDQAPVVLPPWDPFYQAAKRLK"
     gene            771..>1253
                     /locus_tag="PK112_09045"
     CDS             771..>1253
                     /locus_tag="PK112_09045"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HPC12227.1"
                     /translation="MDDFLSRLAGLVPPEQFSTHPDHLRVAAKDESTTTPVTPRAVVW
                     AVTIDEIAAVVRLCRAHRVPITTRGGGSALEGSAVPVEGGIVLDLSRMTAILGYWPED
                     LQVEVQPGLIYDDLNRRLKDDGLFFPPSLGGSGDVATIGGMVSTNASGIYSVKYGGTR
                     D"
BASE COUNT          234 a          381 c          421 g          217 t
ORIGIN      
        1 cggatgtcgc gcagcttcgg gcgcgcaagg aacggatgct ggcggagatc taccgcctcc
       61 tcgtcgccgc ctacggcccg ccgccgaccg agttcacctt ccgctataag gtcaaggact
      121 cggtggtcac cgagccgaaa acctacacgc cgcggtcgtt ctacgacgag atcttcgggg
      181 gaccgctccc cgactacgcc atcctcatgg acaacccggc caaggggtac gacacgctct
      241 accggatcga gagcagccgc aacatggccg accgggcgga cctgaccatg ctcaacctgc
      301 cggtcgcgcg gctcaaggag tattgcctga gagcgctgct cgactcgcag gcggtctggt
      361 tcgcgtgcga cgtcggcgtg gacaatttcg gcgattccgg cattctccgc aacggcatct
      421 atgatttcga ggggacgctc ggggtgtcgt tcgctatcgc caaggccgac cgcatcaccc
      481 tgcgcgacag ctacccgaac cacgccatga cgctggtcgg ggttgacacc gccgcggatg
      541 ggtcgcccgt caagtggctc gtggccaaca gctgggggga caaaggaggc aacgacggtt
      601 actggatcat gtacaacgac tggtttgaca ccaacgtgta cctggtggcg gtggagaagc
      661 ggctgctggc ggaggccgac cggaaaaagc tcgaccaggc gccggtggtg ctgccgccct
      721 gggatccgtt ttaccaggcc gccaagcgcc tgaagtaggc ggcgcggacc atggatgatt
      781 ttctgagccg actggcgggg ctggttccgc cggagcagtt cagcacccac cccgaccatc
      841 tgcgggtggc ggcgaaggat gaatcgacga cgacgccggt gacgccgcgc gcggtggtct
      901 gggctgtgac aatcgacgag atcgcggccg tggttcgcct ctgtcgcgct caccgggttc
      961 cgattacgac ccgcggggga gggagtgcgc tggagggatc ggctgtaccg gtggagggag
     1021 gaattgtgct cgatctcagc cggatgacgg cgattctcgg atactggccg gaagacctgc
     1081 aagtcgaagt acaaccagga cttatatatg atgacctgaa ccgcaggttg aaagacgacg
     1141 ggttgttctt ccctccctca ctgggcggct cgggtgatgt agcgaccatc ggcggcatgg
     1201 tctcgaccaa cgccagcggc atctattcag tgaaatacgg cggcacgcgt gat
//
LOCUS       DAOOKS010000619         1249 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_147460_length_1249_cov_2.435511, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000619 DAOOKS010000000
VERSION     DAOOKS010000619.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147460_length_1249_cov_2.435511"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="PK112_09050"
     CDS             complement(<1..367)
                     /locus_tag="PK112_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12228.1"
                     /translation="MASATDTPAPVPAAPPAAPILFVDFDGDGINDNFVDANRDGIPD
                     EPEPIASTAGIFSGDTRFASPSFALAARNSDVFNQRLSRLTCLTLNRGGFGSAENFGP
                     GNGISAGAVSSGTCVGGVCH"
     gene            complement(634..>1249)
                     /locus_tag="PK112_09055"
     CDS             complement(634..>1249)
                     /locus_tag="PK112_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12229.1"
                     /translation="WGPHGSPQADMYIGTNGYEYDDWDYSPSRHPSVIEDGCVACHMA
                     DPALWAPVGGHTFNIAGEVEVEDEPGMFEEVTNFANSCGGCHESFATINPVFPADIDY
                     DYDGTSEGVQDEIHGLLDSLGVLLLNAGLVIEEDGLLEPDPEVTTSADSAGAVWNYLF
                     VESEGSHGIHNTNYAVALLQSAIAYLAGDTGPALAGRPSLFSAH"
BASE COUNT          253 a          352 c          426 g          218 t
ORIGIN      
        1 agtggcacac gccgccaacg cacgtgccgc tgctgaccgc gccggcgctg atgccgttac
       61 cgggaccgaa attctcggcc gaaccaaacc cgccgcggtt gagcgtgagg caggtcaggc
      121 ggctgagccg ttggttgaaa acatcggagt tgcgggccgc cagggcaaac gatggagagg
      181 caaaccgggt gtcgccggag aagatgccgg cggtcgaagc aatcggctcg ggctcgtccg
      241 ggataccgtc gcgattggcg tcgacgaaat tgtcattgat gccatcgccg tcaaaatcga
      301 caaagagaat cggagcggca ggaggggcgg caggaacagg ggccggggtg tccgtcgcgg
      361 atgccacact gacgagtatc ggaaacacaa ccgagagcac tgagcgaatt cccaaagacg
      421 tctcctttcg cgatgcgagt ctcagcacaa ctcaatttgc gacgagcgga tcgcggctgt
      481 atccaatact tgataataag gtacatttga ccaaattcca aactatttct ggggtttttt
      541 gcggtagtgc ccccaatcgc aaagattgac gacgcgtaac gggaaagccc ctccgggggg
      601 aggggccttc gcgcaaaagc gggacgaaac ctgtcagtgg gccgagaaca gggagggccg
      661 tccggccaaa gccggaccgg tatcgccggc caggtaggca attgccgact ggagcagggc
      721 caccgcgtag ttggtgttgt gaatgccgtg cgagccttcg ctctcaacaa acaggtagtt
      781 ccagactgcc ccggccgagt ccgccgacgt ggtcacctcg gggtcgggct cgagcaggcc
      841 gtcttcctcg atcaccaggc cggcgttgag caggagaacg ccgaggctgt cgagaagacc
      901 gtggatttcg tcctgaacgc cctcggaggt gccgtcgtag tcgtagtcga tgtcggccgg
      961 gaagacgggg ttgatcgtgg cgaaggactc gtggcagccg ccgcaggagt tagcgaaatt
     1021 ggttacttct tcaaacatcc ccggctcatc ctcgacttcg acttcaccgg cgatgttgaa
     1081 ggtgtggccg ccgacggggg cccagagggc gggatcggcc atgtggcagg cgacgcagcc
     1141 gtcttcgatg acgctggggt ggcgggacgg gctgtagtcc cagtcgtcgt actcgtaccc
     1201 gttggtgccg atatacatgt cggcctgcgg gctgccgtgg ggtccccag
//
LOCUS       DAOOKS010000620         1245 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_148143_length_1245_cov_2.647059, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000620 DAOOKS010000000
VERSION     DAOOKS010000620.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148143_length_1245_cov_2.647059"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            135..770
                     /locus_tag="PK112_09060"
     CDS             135..770
                     /locus_tag="PK112_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012553561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HPC12230.1"
                     /translation="MKLYDYPQAPNPRRVNIFLAEKGIQVERVPIDLMKGEQLTPEYR
                     AKNPACDIPMLELDDGTCISQIRAICRYFEETHPATPLLGRTPTEKALCEMWEHLAFM
                     NGLLAVAEVFRNTAKGFADRAVVGPHNYPQSAELAERGRARVANFFTDFDRQLQGRDY
                     VAGNFYSMADITTLVTCDFAKWIKAEILAQCTHLKAWYDRIAARPAVQANR"
     gene            916..>1245
                     /locus_tag="PK112_09065"
     CDS             916..>1245
                     /locus_tag="PK112_09065"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HPC12231.1"
                     /translation="MSLDQRQFRDALGAFPTGVTVVTARDVDGKPVGATANSFSSVSL
                     DPPLVLWSVARTANCYNAFMKAKYYAIHVLHSGQENISRLFATKEADKFANLKWHENA
                     NGAPLLDD"
BASE COUNT          235 a          455 c          363 g          192 t
ORIGIN      
        1 ctgcggtgtt tcatcccgtg gtctcgtatt cgatggtgcc ggccgctgtt accatgcggc
       61 catgccatgc gcaacgtccg ccggatcgcg atccggacgg cagccgtttc cgacagccac
      121 gaggataagc ccagatgaaa ctctacgact acccgcaggc cccgaacccc cgccgcgtga
      181 acatctttct cgccgaaaaa ggcatccagg tggagcgggt gcccatcgac ctgatgaaag
      241 gcgagcagct cacccccgag taccgcgcca agaatcccgc ctgcgacatt cccatgctgg
      301 agctggacga cggcacctgc atcagccaga tccgcgccat ctgccgctac ttcgaggaaa
      361 cccacccggc cacgccccta ctcggcagaa cgccgaccga gaaggcgctg tgcgagatgt
      421 gggaacacct cgccttcatg aacggcctgc tcgccgtcgc cgaggtattc cgcaacacgg
      481 cgaagggctt tgccgaccgc gccgtggtcg gcccccacaa ctatccgcag tcggcggaac
      541 tcgccgagcg cggtcgcgcc cgggtggcga atttcttcac cgacttcgac cggcagctcc
      601 agggccgcga ctacgtcgcc ggcaacttct actcgatggc cgacatcacc acgctggtga
      661 cctgtgattt cgccaaatgg atcaaggccg agattctcgc gcaatgtacc catctcaagg
      721 cctggtacga ccgcatcgcc gcgcgccccg cggtacaggc caaccgctga cccgccgcgg
      781 gtgcggcagc gccgggggct gtgctaaatt gacccgcctc ccaggcgggt gatgcgcgcc
      841 cggctccggc cgggaccggc gccgcacccg ccggcaagtt cacgcaacgc gccatccaac
      901 cagaggggaa tcatcatgag tcttgatcag cggcaattcc gggacgcgct cggcgccttt
      961 cccaccggcg tcaccgtcgt caccgcccgg gacgtggacg gcaagccggt cggcgccacc
     1021 gccaacagct tttcctcggt ctcgctggac ccgccgctgg tgctgtggag cgtcgcccgg
     1081 accgccaact gctacaacgc cttcatgaag gcgaagtact acgccatcca tgtgctgcat
     1141 tccgggcagg aaaacatcag ccgcctgttc gccaccaagg aagccgacaa gttcgccaac
     1201 ctcaagtggc acgaaaacgc caacggcgcg ccgctgctcg acgat
//
LOCUS       DAOOKS010000621         1226 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_151741_length_1226_cov_4.037575, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000621 DAOOKS010000000
VERSION     DAOOKS010000621.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151741_length_1226_cov_4.037575"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..837)
                     /locus_tag="PK112_09070"
     CDS             complement(<1..837)
                     /locus_tag="PK112_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HPC12232.1"
                     /translation="MKKVLIGGALLLVAGVALSAGAITMKGSDTLVRLGQRWAEEYMK
                     QNPGTVIQVSGGGSGTGIAALLNGSTDICQASRDMKEKEYQLAEKNGKEPFRVAVALD
                     GIAVFLNAKNPVGELTVPQLKGIYTGAIVNWKEVGGPDAPIILYGRENDSGTYGFFKE
                     HVLNDEDYADRTQTLPGTAAVVNAVGKDPNGIGYGGLAWATGVKFAAVRQNDTAAAVM
                     PSLETVSDGRYPISRDLYWFFNGPPTGELKKLLNWVLSPAGQTMAADIDYVPLPAERA
                     RAN"
     gene            complement(933..>1226)
                     /locus_tag="PK112_09075"
     CDS             complement(933..>1226)
                     /locus_tag="PK112_09075"
                     /inference="COORDINATES: protein motif:HMM:NF028240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HPC12233.1"
                     /translation="NAGWSLGASAGKIKNPGSWTVRYVYRELEADAVVGAFTDSDLGE
                     GGANASGHEVSAACQVAGNTTFSATYFYNTIGIGASDKAEYLNRLQVDLQLKF"
BASE COUNT          190 a          399 c          385 g          252 t
ORIGIN      
        1 gttggctctg gctctctcgg cgggcagcgg cacgtagtcg atgtcggccg ccatggtctg
       61 accggcgggc gagagcaccc agttgagcag tttcttcagc tcgccggtcg gggggccgtt
      121 gaagaaccag tagaggtcgc gcgaaatggg ataccggccg tccgagaccg tctccagcga
      181 gggcatcacg gcggcggctg tatcgttttg ccgaaccgcc gcgaacttca cgcccgtcgc
      241 ccacgccaga ccgccgtagc cgatcccgtt gggatccttc cccaccgcgt tcaccaccgc
      301 ggccgtcccc ggaagcgtct gcgtccgatc ggcgtagtcc tcatcattca ggacgtgctc
      361 cttgaagaag ccgtaggtgc cggagtcgtt ttcgcggccg tagagaatga tcggagcgtc
      421 cggtccgccg acctctttcc agttgacgat ggcgccggtg tagatgcctt tgagctgggg
      481 cacggtgagc tcgccgaccg ggttcttcgc attgagaaac acggcgatgc cgtcgagcgc
      541 gaccgccacc cggaatggct ccttgccgtt cttttcggcc agttggtact ctttctcctt
      601 catgtcgcgg gaggcctggc agatgtcggt cgatccgttg agcagggcgg cgatccccgt
      661 gcccgagccg ccgccggaaa cctggatcac cgtccccgga ttttgcttca tatactcctc
      721 ggcccagcgc tggccgaggc ggacgagcgt gtcggaccct ttcatcgtga tggcgccggc
      781 gctcagcgcc actccggcca cgagcagcag ggcgccgccg atcagtacct tcttcatcgt
      841 gttctctcct ctgccttcca tctggcgttc cgatgacata acatttgcat tgttaggatc
      901 ttgtgaggat gcctcttgca cgggtgtgcc cctcagaatt tgagctgcag gtcgacctgc
      961 agccggttta agtactcggc cttgtcggac gcaccgattc cgatggtgtt atagaaatag
     1021 gtggcgctga acgtagtgtt cccggccacc tggcaggcgg ctgagacctc gtggccggag
     1081 gcgttggcgc cgccctcgcc caagtcggaa tcggtgaacg ccccgacgac ggcatcggcc
     1141 tcgagctcgc ggtagacata ccgcaccgtc cacgagccgg gattcttgat tttgccggcc
     1201 gacgccccga gcgaccagcc ggcatt
//
LOCUS       DAOOKS010000622         1222 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_152516_length_1222_cov_3.429306, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000622 DAOOKS010000000
VERSION     DAOOKS010000622.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152516_length_1222_cov_3.429306"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="PK112_09080"
     CDS             <1..598
                     /locus_tag="PK112_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12234.1"
                     /translation="YRFQGYEDSKVYRDENATGVFIGYGINAVRLWDEFEKRGQLDSA
                     MAIADKIMKEYPEYAQMYMIVSNELRQQGDTAAADSLLRQAHDTLTAFCRSNPENLFY
                     LSDLGLIKVSIGSISGRQDLLDDGLALMWEAFRANPESGTAFRKLFYALSDQRRYAEI
                     QEAARLHASYPRNLEDPFLQQLLGLTQSTPRIPPGAGQ"
     gene            complement(675..1157)
                     /locus_tag="PK112_09085"
     CDS             complement(675..1157)
                     /locus_tag="PK112_09085"
                     /inference="COORDINATES: protein motif:HMM:NF021386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HPC12235.1"
                     /translation="MDYYEGVIKNTEFFTTTELADKLKMNVQVITRKVQAGEITAYKI
                     GKDWRIPEQSVYEWLQQRSNRNGAAAAPAPRRNIRLKRSAETETVPPSRRTKRKYLLE
                     YILAQFEPNRLYEDHEVNRVISRFNEDPVAVRTDFLEEHMMEQVGGRLRRSAGYKLSE
                     "
BASE COUNT          210 a          392 c          382 g          238 t
ORIGIN      
        1 ctaccgcttc caggggtacg aggactcgaa agtgtaccgg gacgagaacg cgaccggcgt
       61 gtttatcggc tacggcatca acgccgtgcg gctctgggat gaattcgaga agcgcgggca
      121 gctggactcg gccatggcca ttgcggacaa gatcatgaag gagtaccccg agtacgcgca
      181 gatgtacatg atcgtgtcga acgaactgcg ccagcagggc gacacggcgg cggccgattc
      241 gcttctgcgc caggcgcacg acacgctcac cgcgttctgc cgctccaacc cggaaaacct
      301 gttctacctg agcgacctcg ggctcatcaa agtcagcatc ggctcgatct ccgggcgcca
      361 ggatctgctc gacgacgggc tcgctctcat gtgggaggcg ttccgggcca accccgaaag
      421 cggcacggcc ttccggaaac tcttctacgc cctcagcgac cagcggcgct acgcggaaat
      481 ccaggaggcg gcccgcctgc atgcctcgta tccgcgcaat ctcgaggatc cgttcctgca
      541 gcagctcctc ggcctgaccc agtccacccc ccgcatcccc ccgggggcgg gccagtagcg
      601 gacccggccg ccggaaaaca aaaacctccc gcacgcccgt gcgggaggtt gtttctttca
      661 cgcaaccggc cggcctattc ggagagcttg taaccggcgc tgcggcggag cctccccccc
      721 acctgctcca tcatgtgttc ctcgaggaaa tcggtgcgca cggcgaccgg gtcctcgttg
      781 aaccgggaaa tgacccggtt gacctcgtga tcctcgtaga gccggttcgg ttcaaactgg
      841 gcgagaatgt actcgaggag gtatttccgt ttggtgcggc ggcttggcgg caccgtctcg
      901 gtttcggccg agcgcttgag acggatattc cggcgggggg ccggggcggc cgcagccccg
      961 ttgcggttgg agcgctgctg cagccactcg tacacggact gctccgggat gcgccagtct
     1021 ttcccgatct tgtaggcggt gatttcgccg gcctggactt tgcgcgtgat gacctggacg
     1081 ttcatcttca gcttgtcggc cagctcggtg gtggtgaaga actcggtgtt ctttatgacg
     1141 ccctcgtagt agtccacttg cgctctccat catctttagg cgttgcggaa cgtgtcatat
     1201 cctcgcacag agtagtgtag tt
//
LOCUS       DAOOKS010000623         1216 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_153680_length_1216_cov_3.757967, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000623 DAOOKS010000000
VERSION     DAOOKS010000623.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153680_length_1216_cov_3.757967"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="PK112_09090"
     CDS             complement(<1..888)
                     /locus_tag="PK112_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12236.1"
                     /translation="MIAGLLTGLSLLNHLISPSSFHLSAEAWDDLLAYHRDFDVFRVR
                     PVTTAVVGFLAEQTGLSVRSAFFLLQFALFFVTGPAFYHFLRSLQFSPARSRAGMVIL
                     LLSLPLFLAHFEPVWTWDDFWCYILLPSAMAFALGRRYWPAAACIAGAMIARETSAAF
                     LPVLFLLFRTNPKVGIARPLLATLAPVAVLVVFRAAVLGAVSGSPVWSLAFNFESWAR
                     TRDSLFSLIVSLGFLWPVGLTQAFRRPEESPAWYDAVRFGALLTAGAYLVSGLLFGHI
                     RESRLLLPPALLFIPLVLVY"
BASE COUNT          270 a          441 c          368 g          137 t
ORIGIN      
        1 atatacgagc acgagcggaa tgaacagcaa cgccggcggc agcagcaggc gactctccct
       61 tatatggcca aagagaagac ccgacaccag gtacgcgccg gccgtcaaca gcgccccgaa
      121 tcgcaccgcg tcgtaccaag cggggctctc ctccggccgg cggaacgcct gagtcagccc
      181 caccggccac aggaaaccca gcgacacgat cagcgagaac agggagtcgc gcgtccgcgc
      241 ccagctttcg aaattgaagg ccagcgacca cacgggcgag ccgctgaccg cgccgagcac
      301 agccgcccgg aacacgacca gcaccgcgac cggcgccagc gtcgccagaa gcgggcgggc
      361 aatcccgact ttcgggttgg tgcggaacag gaggaacagc accggcagaa aggccgcgct
      421 tgtctcgcgg gcaatcatgg ctcccgcaat gcaggccgcc gccggccagt accggcgacc
      481 cagggcaaag gccatcgccg acgggagcag gatgtagcac cagaaatcat cccatgtcca
      541 caccggctcg aagtgggcca ggaacaatgg aagcgacagc agcagaatca ccatacccgc
      601 ccgactccgt gccgggctga actgcagcga ccgaaggaaa tggtagaacg ccggaccggt
      661 gacgaagaag agcgcgaact gcagaaggaa aaacgcgctc cgcaccgaca gccccgtctg
      721 ctccgccagg aaaccgacca ccgccgtggt caccggccgc acccggaaca cgtcaaaatc
      781 ccggtggtag gcgagcaaat cgtcccaggc ctcggcggag agatggaacg aactgggact
      841 gatcaggtgg ttcagcagac tcagtccggt cagcagcccg gcaatcaacc agaccgcgcg
      901 cgtttccgat gaacctgaca cccggataga cctcgttccc ctgtgatttc ggcccgactc
      961 gccgccgcac cgccacatgc ctgcagccgt acgggcgagt gatgatagag agcgccgggc
     1021 gcgcgccgca agcaaaaacg gcgcaatcgg cccggcgccg ccgccccccc cggcctatcc
     1081 gcccgcccga tctccttgcc ccgcgccgcc ccgtcgatta tcttccctct gaaatggcgg
     1141 ttcaaccggc cgggcaatcc cccttcgtag tcaggccgac cggtcccccc gcctcaggcg
     1201 gagcgctgac gtgcac
//
LOCUS       DAOOKS010000624         1216 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_153726_length_1216_cov_2.756245, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000624 DAOOKS010000000
VERSION     DAOOKS010000624.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153726_length_1216_cov_2.756245"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            261..>1216
                     /locus_tag="PK112_09095"
     CDS             261..>1216
                     /locus_tag="PK112_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015809619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HPC12237.1"
                     /translation="MFEKVLIANRGEIALRVIRACREMGLKTVAVYSEADRDALHVRF
                     AEEDVCIGPPPARQSYLDFKHIIAAAEVTNAGAIHPGYGFLAENADFAEICESCGLAF
                     IGPTPEQIRQLGDKVQAKDLMRKAGVPCIPGSEGAVATFREAATVAEEVGYPVILKAV
                     AGGGGKGMRICRDVAELERGFHVASAEAGNAFGNPDLYLEKVVLNPHHVEIQVIGDTY
                     GNYLHFGERDCSIQRRHQKLIEETPSPLMTPELRRRMGEAALNGAAQVGYRGVGTMEF
                     LVDDQRNFYFMEMNTRIQVEHPVTEEAYEVDLLQDQIRVALG"
BASE COUNT          242 a          353 c          403 g          218 t
ORIGIN      
        1 cgcgccgatc cggacaaagc accgcttatc cagagacacc ccgatggggg tgtctctttt
       61 ccttatgcgc atcctctgcg cctctttgtt agtgattgtg cgacaatatc ttatcgtccc
      121 agaaaggggg gaacaccgga cgcgcgggag cgtttattga gttgaattcg ggggacgaat
      181 agggtataat acctatctgt gacgtgcggt ccgggcagca ccctgtccgg gctgtcaacg
      241 aaaagacgaa aggactgtgc ttgttcgaga aagtactcat tgccaatcga ggcgaaatcg
      301 ctctccgggt gatccgggcg tgccgtgaga tggggctgaa gacggtcgcg gtgtacagcg
      361 aagccgaccg cgatgccctc cacgtgcgct tcgccgaaga ggatgtatgt atcgggccgc
      421 ccccggcccg gcagtcgtat ctcgatttca agcatatcat tgcggcggcg gaggtgacca
      481 acgccggcgc catccacccc ggctatggtt ttttggcgga aaacgccgat ttcgcggaga
      541 tttgcgagtc atgcgggctg gcgttcatcg gcccgacgcc ggagcagatc cggcaactgg
      601 gcgacaaggt ccaggccaaa gacctgatga gaaaggccgg cgtgccctgc attccgggat
      661 cggagggtgc ggtggcgacg ttccgggaag ccgccacggt ggccgaagaa gtcggctacc
      721 ccgtcatcct gaaagcggtc gccggcggcg gcggcaaggg gatgcggatt tgccgcgatg
      781 tggcggaatt ggagcgcggc ttccatgtcg cctcggccga ggccgggaac gccttcggca
      841 accccgatct ctacctggag aaagtcgtgc tcaatcccca ccacgtcgag atccaggtga
      901 tcggcgacac ctacggcaac tacctgcatt tcggcgagcg tgactgctcg attcaacggc
      961 ggcaccagaa gctgatcgag gagacgccct caccgctcat gacgcccgag ctgcggcggc
     1021 ggatgggcga agcggcgctc aacggcgccg cccaggtcgg ataccgcggg gtggggacga
     1081 tggagttcct cgtcgacgat cagcgcaatt tctacttcat ggaaatgaac acgcggatcc
     1141 aggtggaaca tccggtgacc gaagaggcct acgaagtcga cctcctgcag gaccagatcc
     1201 gggtggcgct gggcga
//
LOCUS       DAOOKS010000625         1207 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_155591_length_1207_cov_2.631076, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000625 DAOOKS010000000
VERSION     DAOOKS010000625.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155591_length_1207_cov_2.631076"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="PK112_09100"
     CDS             complement(<1..644)
                     /locus_tag="PK112_09100"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HPC12238.1"
                     /translation="MTGSNISFEFFPPRTPEAVSRLETTRARLAAYGPSFFSVTFGAG
                     GSTRELTLETVLAIRRATGIEAAPHISCLGTPIADLRAVLRRYVDEGIKRVVALRGDM
                     PSGLTGFGELRYANELVAFIRAETGRQFHIEVAAYPEFHPQAPSAKADLANFKRKVEA
                     GADSAITQYFFNADAYFRFVDSVRALGVEIPIVPGIMPVTNFSQLARFSEMCGA"
BASE COUNT          198 a          395 c          427 g          187 t
ORIGIN      
        1 tcggcgccgc acatctcgga gaaacgcgcg agctggctga agttcgtgac cggcatgatg
       61 ccgggcacga tcggaatctc cacgccgagc gcccggacgc tgtcgacgaa acggaagtag
      121 gcgtcggcgt tgaagaagta ctgcgtgatc gcgctgtccg cgccggcctc gaccttgcgc
      181 ttgaaattgg cgaggtcggc cttggccgac ggcgcctgcg ggtggaattc ggggtaggcc
      241 gcgacctcga tgtggaactg gcgcccggtc tcggcgcgga tgaacgcgac gagttcgttc
      301 gcgtagcgca gttcgccgaa gccggtgagg ccggacggca tgtcgccacg gagcgcgacg
      361 acccgcttga tgccctcgtc gacgtagcgg cgcagcaccg cgcgcagatc ggcgatcggc
      421 gtgccgaggc aggagatgtg cggggccgcc tcgatgccgg tcgcgcgacg gatcgcgagc
      481 actgtctcga gcgtcagctc gcgcgtgctg ccgccggcgc cgaaggtcac ggagaaaaac
      541 gacgggccgt acgcggcgag ccgcgcgcgg gtggtctcca gccggctgac cgcctcgggc
      601 gtccgcggcg gaaagaattc aaaactgatg ttgctgcccg tcatcgccat gctctgcctg
      661 tgatgccgcc ggtgagggag cggcaagacc tgggccggca ctcgggcagt ccggccccgc
      721 gcctgcgccg ggagcttctt tttgtgcggc aggcaattgg gccgaatata ccgtactgat
      781 aaggagatgt aaccatatat tcaacttcta cctgagtagg gaaccccgac ttggacccac
      841 cggagtcggc ggccagttta gcgtgcgcgt caggcatggt ccgtgatcgg cgcggcgctg
      901 tcgcgaacgg gtccgcgagc gccgagggct gcgggaggcc ttcctgccct gcccccgtcg
      961 ggagactcgg tccggcagcg ccgatggatg ccggcatcgg acaccagctc cgtcgatgcg
     1021 ccggtccgaa acgacgaccg gcacgacgaa atgtcccgca ccgcgcgggg tttgcttttg
     1081 cggcgaccgg cctggcagta gaatcccgct tctctctccg gcccgcccgg gttcgcccgg
     1141 cggcagcact ccaaacgata agcccccagg cccgaggacc tcagcatgag cacatccgca
     1201 gcggcag
//
LOCUS       DAOOKS010000626         1203 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_156376_length_1203_cov_4.234321, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000626 DAOOKS010000000
VERSION     DAOOKS010000626.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156376_length_1203_cov_4.234321"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..145)
                     /locus_tag="PK112_09105"
     CDS             complement(<1..145)
                     /locus_tag="PK112_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase (FAD)"
                     /protein_id="HPC12239.1"
                     /translation="MAHTENKAADDLLDREFRVLDHGFVRLIDYMGGDESIVQAARVS
                     YGKG"
     gene            311..1186
                     /locus_tag="PK112_09110"
     CDS             311..1186
                     /locus_tag="PK112_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12240.1"
                     /translation="MIALRRPPEGRTRLAWHELEQIQTNEWVLKLRPPYYGAVFADRE
                     PDLAAKSPAGFHIARTGAVAWAVFRGLTPRDLARINDFAGAYRRFVLLGDNPNLKGQF
                     SDELDGCLALDYNFAESRSRSYTEIGELVYHAKYRRSVAAAEELGRRLAEAAGRVPTM
                     RCVSRPCLSYVPPGPHKRFDLPRILARLLVDYGWCTMPLREFEPLVHPELSLAKPDFK
                     RLTIDEKIRVWTRLLARGVRLSGNVAGAAVYVIDDLYQSGATLWSYARYLKAAGAAAV
                     FGLVCEKSRGDRDNR"
BASE COUNT          197 a          445 c          362 g          199 t
ORIGIN      
        1 tccccttgcc gtaggacacc cgcgccgcct gcacgataga ttcatccccg cccatgtaat
       61 cgatcagccg cacgaagccg tggtcgagca cccggaattc cctgtcgagc aggtcatcgg
      121 cggctttgtt ctcggtatgc gccatgtgat tctccggagt ttgcccccga atatagcgcg
      181 gccgccgggg cggggcaaac ggtttgcacg agggcccggc aaaatgtgat tgccccccgc
      241 ctccccgccc gctatcgtca gcccggacgc acgaccaagg cccgaccacg aacttgtccc
      301 ggggaggaac atgatcgcac ttcggcgccc gcctgaaggc cgcacgaggc tggcctggca
      361 cgaactcgag cagatacaga ccaacgagtg ggtcctcaag ctccgccccc cgtactacgg
      421 cgccgtgttc gccgaccgcg agccggacct ggccgccaaa tcccccgccg gattccatat
      481 cgcccggacg ggcgcggtcg cctgggcggt gtttcgcggg ctcaccccgc gcgacctcgc
      541 ccgcatcaac gacttcgccg gcgcctaccg ccggttcgtc ctccttggcg acaacccgaa
      601 cctcaagggg cagttctccg atgagctcga cggctgcctg gcccttgact acaatttcgc
      661 cgaaagccgc agccgcagct ataccgagat cggcgaactc gtctaccacg ccaagtaccg
      721 ccggtcggtg gccgcggccg aggaactcgg gcggcggctg gccgaggcgg ccggccgcgt
      781 accgaccatg aggtgcgtct cccgcccctg cctctcgtac gtgccgccgg gaccgcacaa
      841 gcggttcgat ctcccccgga ttctcgcccg gctgttggtc gattacggct ggtgcaccat
      901 gcctctgcgt gaattcgagc cgctcgtcca ccccgagctg agcctggcca agcccgattt
      961 caaacgtctg accatcgacg agaaaatccg cgtctggacc cggctccttg cccgcggagt
     1021 gcgcctctcg ggcaatgtcg ccggcgccgc cgtctatgtg atcgatgacc tctaccagtc
     1081 gggcgcgacc ctatggtcat atgcccggta cctcaaggcg gccggggctg ccgccgtctt
     1141 cgggctggtc tgcgagaaat cgcgaggcga ccgggataac cgatgaaccg cgaaaccgcc
     1201 ctc
//
LOCUS       DAOOKS010000627         1200 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_156971_length_1200_cov_3.791266, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000627 DAOOKS010000000
VERSION     DAOOKS010000627.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156971_length_1200_cov_3.791266"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="PK112_09115"
     CDS             complement(<1..573)
                     /locus_tag="PK112_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HPC12241.1"
                     /translation="MNAPFAAPAENYRVDLPVFNGPLDLLLYLIKKEEVDIYDIPIAR
                     ITQQYLKYMELMKELNLEVAGEFVVMAATLIHIKVRLLLPRDPDQAEEDDPREELIMA
                     LLEYKKYREASEILREKAIMEERYFVPELPVGRPDLKVDLSPGTTLFDLLTAFREVLA
                     NRSDEQVHRVDTFEVSIEDRIGHVMRILRGR"
     gene            complement(577..>1200)
                     /locus_tag="PK112_09120"
     CDS             complement(577..>1200)
                     /locus_tag="PK112_09120"
                     /inference="COORDINATES: protein motif:HMM:NF012788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HPC12242.1"
                     /translation="YGFLGYPVLQAADICVYNAHKVPVGKDQAKHVWLANDLAKRFNH
                     YYGPTLNEPEGLYREIPLILGSDNRKMSKSYDNHIPLDFTEEETAKRLRTFYTDSQKI
                     RLGDPGRPEQCPIYLLHRIYTPGAEESVAAPCRSGALGCVECKKRLADNLNAALRPIR
                     ARRQELLRRPGDVWDVLADGARRARERAAEVMEKIHTTMKLNYRKQG"
BASE COUNT          191 a          368 c          399 g          242 t
ORIGIN      
        1 gcgcccgcgc aggatgcgca tgacgtggcc gatgcggtcc tcgatcgaca cctcgaaagt
       61 gtcgacccgg tggacctgtt catcggagcg gttagcgagc acctcccgga aggccgtgag
      121 gaggtcgaag agggtggtgc ccggggagag atccaccttg aggtccggcc gcccgaccgg
      181 cagctcgggg acgaagtagc gctcctccat gatcgctttc tcgcggagga tttcgctcgc
      241 ctcgcggtac ttcttgtact cgaggagggc catgatcagt tcttcgcgcg ggtcgtcctc
      301 ctcggcctgg tccgggtcac gcgggagcag gaggcggacc ttgatgtgaa tgagggtcgc
      361 cgccatgacc acgaactcgc ccgccacctc gagattgagc tccttcatca gctccatgta
      421 cttcaggtac tgctgggtga tgcgggcgat cgggatatcg tagatgtcga cctcttcctt
      481 tttgatcagg tagagcagca ggtcgagcgg accgttgaag acggggaggt cgacgcggta
      541 gttttcggcg ggtgcggcaa atggtgcgtt catgcctcag ccttgttttc ggtagttcaa
      601 tttcatggtg gtatggatct tctccatgac ctcggcggcc cgctcgcggg cgcgccgcgc
      661 gccgtcggcc aggacatccc acacgtcgcc ggggcgccgc agcagttcct ggcggcgggc
      721 gcggatcggc cgcagggcgg cattcagatt atcggccagc cgcttcttgc actcgacaca
      781 gcccagcgcc ccgctccggc agggcgcggc caccgactcc tcggcccccg gcgtgtagat
      841 gcggtggagc aggtagatcg ggcactgctc cggccgtccc ggatcgccca ggcggatttt
      901 ctgcgagtcc gtgtagaatg tccgaagccg cttggccgtc tcttcttccg tgaaatccag
      961 cgggatgtgg ttgtcgtacg acttcgacat cttccgattg tccgaaccga ggatgagcgg
     1021 aatctcgcga tacagcccct ccggctcatt cagcgtcgga ccgtagtagt ggttgaaccg
     1081 cttggccaga tcgttcgcca gccagacgtg cttggcctgg tccttgccga cagggacctt
     1141 gtgcgcgttg tagacgcaga tgtcggccgc ctgcagcacc ggatagccga ggaaaccgta
//
LOCUS       DAOOKS010000628         1186 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_159956_length_1186_cov_3.264368, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000628 DAOOKS010000000
VERSION     DAOOKS010000628.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159956_length_1186_cov_3.264368"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1013
                     /gene="murB"
                     /locus_tag="PK112_09125"
     CDS             <1..1013
                     /gene="murB"
                     /locus_tag="PK112_09125"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HPC12243.1"
                     /translation="RPRTWLFLLDFQGFCEYIPVVRSTQRAVPRAQVSTAEMRSAFGP
                     ELEFDKPMAPLTSFRTGGTAAWFLPVRSAEAAAGAVQQARRMGLEFFLLGGGSNLLVA
                     DRGYGGLIIKMDVRGLARVDEATIECGAGEDLTALVTYAQENGLAGLEFAAGIWGTVG
                     GAIYGNAGAYGGEIGEVVTAVTLVDREGKVMTVDRAYCGFGYRDSALKRSHEIVVSAR
                     FRLQPGDPAEIGRRVREILASRAQKHPDRLTAGCFFKNIPDPREPYGKLPAGRLLEEA
                     GAKGLAVGGARVFERHANIIVNAGGATAADIAALAALMKEKVRERFGIELQEEVIRLG
                     EF"
BASE COUNT          197 a          316 c          454 g          219 t
ORIGIN      
        1 cgcgaccccg gacgtggctg tttctccttg attttcaagg attttgcgag tatattccgg
       61 tggtgagatc aacccaacga gcggtgccgc gggcgcaggt gtctacggcc gagatgcgca
      121 gcgcattcgg gccggaactc gaatttgaca aaccaatggc gccgttgacg agtttccgca
      181 cgggcgggac agcggcgtgg tttttgccgg tgcgctcggc ggaagccgcc gccggggcgg
      241 tgcagcaggc ccgccggatg ggtctggagt tttttctgct gggcggagga tctaacctgc
      301 tggtggcgga ccggggctat ggcggtctga tcattaaaat ggatgtgcga gggctggcgc
      361 gggtcgatga ggcgacgatc gagtgcgggg cgggggagga tttgacggcg ctcgtgacgt
      421 atgcgcagga gaacggtctg gccgggctcg aattcgccgc cggcatctgg ggcaccgtcg
      481 ggggagccat ctacggcaac gccggcgcct acggcggcga gatcggcgaa gttgtcacgg
      541 ccgtgacgct ggtggaccgc gaggggaagg tcatgacggt ggaccgcgcc tattgcgggt
      601 tcggctaccg ggactcggcg ctcaagcgct cgcatgagat tgtcgtcagc gcgcgcttcc
      661 gtctgcagcc cggcgatccg gcggagatcg gccggagggt gcgcgagatt ttggcctcgc
      721 gggcgcagaa acaccccgac cgtctgacgg cgggttgttt tttcaagaat attcccgatc
      781 ccagggagcc ctacgggaaa ctcccggcgg ggcggctgct cgaagaagcg ggggccaagg
      841 ggctggcggt cgggggggcg cgggtgttcg agcgccatgc gaatattatc gtgaacgccg
      901 gcggggccac ggcggccgac attgcagcgc tcgcggcgct catgaaggag aaggtccggg
      961 agcggttcgg gatcgaactg caggaagaag tgatcaggct gggtgaattc tgagggaaag
     1021 gaggacgatt tatcgagtag tgactaacga ctgaagtatc attctggcac cagagtggtt
     1081 gtgaaagcgt tctctgcatg gcgccgtctg tacgcgccgt tgattttctg cctcgcgctg
     1141 ccggcggccg gttccgcatg ggccgcatcg ggaccccggt cagcgg
//
LOCUS       DAOOKS010000629         1179 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_161492_length_1179_cov_3.331851, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000629 DAOOKS010000000
VERSION     DAOOKS010000629.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161492_length_1179_cov_3.331851"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="PK112_09130"
     CDS             complement(<1..238)
                     /locus_tag="PK112_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12244.1"
                     /translation="MSVAGMRMLSIALSLWILTPPMLSAGRLAPELEERLQGKAAGEK
                     IRVWIGVTGAVDPRALKAALTSSYPDGATRHREGM"
     gene            556..1080
                     /locus_tag="PK112_09135"
     CDS             556..1080
                     /locus_tag="PK112_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6125 family protein"
                     /protein_id="HPC12245.1"
                     /translation="MNLGHLSPDTLRAMLADFAKNWLAHDGLWFQAVERRYGLETAIA
                     LDAEAWEQFTAIEARQIMALHRLPENGGLEALKTALGFRLYAFINEQEILQEERAADG
                     SLVSFEFQMVDCRVQSARRRKGLPLFPCKSVGLVEYEGFARTIDPRIRTQCLCCPPDA
                     AAGADQYCAWRFSL"
BASE COUNT          198 a          419 c          348 g          214 t
ORIGIN      
        1 gcatcccctc gcggtgccgg gtcgccccat cggggtagga tgaagtcaga gcggctttga
       61 gagcgcgggg atcgaccgcc cccgtcaccc cgatccagac cctgattttc tctcccgccg
      121 ctttcccctg cagccgctct tccagctccg gcgccagccg gccggccgac agcatcggcg
      181 gcgtcagaat ccagaggctc agtgctatgg acagcatccg cataccggcg acactcatac
      241 gacccgtcct catttcagaa tcaactccct agtgacgcgt ccggcgcccg attgacgcaa
      301 ccgaatcgag cgcccccgac cgctgcggcg cccttgcggc gccggtgtgg ggcgggtgcc
      361 tgaacagccc ggatgaggac tcgccgaccg cccgtcgtcg gccgaggccc gaacagcctg
      421 ggcggcgcgg cgtcggcggc cggtctctca ccgcttgaga gcaagatact tcctaagctt
      481 gcctttgcca agcggtttgc tgatatttcc gataaacaag agagcgacca ctgcctcacg
      541 aggacacgaa agaccatgaa tctcggccac ctctcgcccg acacgctgcg cgccatgctc
      601 gcagattttg cgaaaaactg gctggctcat gacggccttt ggttccaggc cgtcgagcgc
      661 cgctatggat tggaaacagc aatcgccctc gatgccgagg cctgggagca gttcaccgcc
      721 atcgaggccc gccagatcat ggccctccat cgccttcccg aaaacggcgg cctggaggct
      781 ctcaagaccg cccttgggtt ccgcctctac gccttcatca acgagcagga aattctccaa
      841 gaggagcggg cggccgatgg ctccctggtc tcgttcgagt tccagatggt cgactgccgc
      901 gtacagtccg ctcggcgccg gaaaggcctt ccccttttcc cctgcaaatc ggtcggtctg
      961 gtcgagtacg agggttttgc ccgcacgatc gatccccgca tccggacaca atgcctctgc
     1021 tgtccgccgg acgcggccgc cggcgccgac cagtactgcg cctggcggtt ctcgctttag
     1081 cccgagcccc tgatcggcgc cgaactgcgg tctctccgcg cgttcctcaa gtgtgaccgg
     1141 ggggcttcaa atggcggaag agttcgcccc ggcgttctt
//
LOCUS       DAOOKS010000630         1178 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_161692_length_1178_cov_3.875334, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000630 DAOOKS010000000
VERSION     DAOOKS010000630.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161692_length_1178_cov_3.875334"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="PK112_09140"
     CDS             complement(<1..878)
                     /locus_tag="PK112_09140"
                     /inference="COORDINATES: protein motif:HMM:NF015237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HPC12246.1"
                     /translation="MRHPLAALGGALVLAGGALFIILVLIDLSAAHENMYRSLVTFIL
                     APAVILLGVLLFLISVRLQIGRARRAGEDVRFTLSIDPADPKYIRNLWLFLAFTAVLV
                     LVVVYAGTEAYDATESVVFCGETCHRVMEPQYVTYHNSPHARVACAECHIGPGASFWV
                     KSKVDGLRQVWATALNTYPEPIPTPIVNLRPARETCEQCHWPEQFYGDKLLVRTYYRT
                     DEQNSPWTVALRVKIGGGTPRATGQAGGIHYHMLTERRLEYIPADEKRSVIAWVRSIG
                     PEGDTTVWVNADAEQPA"
BASE COUNT          212 a          377 c          394 g          195 t
ORIGIN      
        1 gccggctgct cggcatcggc gttcacccac acggtcgtgt cgccctccgg cccgatcgag
       61 cggacccagg cgatcacgct gcgcttctca tcggcgggga tgtattcgag ccttcgttcg
      121 gtcagcatat ggtagtggat gccgccggcc tggccggtag cccgcggcgt gccgccgccg
      181 attttgacgc ggagggccac ggtccacggc gaattctgct catcggtccg gtagtaggtg
      241 cggacgagga gcttgtcgcc gtagaactgc tccggccagt ggcactgctc gcaggtttcc
      301 cgggccgggc gcaggttcac gatgggcgtg ggaatgggtt cggggtaggt gttgagggcg
      361 gtcgcccaca cctgccgcag accgtccact ttcgacttca cccagaacga ggcgcccggt
      421 ccgatgtggc attcggcgca ggccacccgg gcatggggcg agttgtggta cgtcacgtac
      481 tgcggctcca tcacccggtg gcaggtttcg ccgcaaaaaa ccaccgattc ggtggcgtcg
      541 tacgcctcgg tgccggcgta cacgaccacg agcacgagca ctgcggtgaa agcgaggaac
      601 agccagaggt tgcggatgta tttcgggtcg gccggatcga tggagagggt gaagcggacg
      661 tcctcgccgg cgcgccgggc ccggccgatc tgcagccgca ccgaaatcag aaacagcagc
      721 acgccgagga gaatcaccgc cggcgccagg atgaacgtca ccagcgaccg gtacatgttc
      781 tcgtgggcgg cggacaagtc gatcaggacg agaatgataa agagggcgcc gccggcaagc
      841 acgagcgcgc cgccgagcgc cgccagggga tggcgcaaga gtgttcgtct gggatggcca
      901 ttcgtagtca ttagtcgatt acctcgttat cgtttgcgac tccgatcggc gccaacatac
      961 ggcaaaacgg ccggcgaatc aagacatgca ggctcatatt tcgccgcaat cagcactttc
     1021 ccgaaagcgg accgcccagt ccgcccgata cgccctgcct ggtcggggtg gcggaccgac
     1081 cggtttgtcc ggtcggtccg cacccgcgcc agatcagaag ttcaccagca ctcccgaccg
     1141 gaccatatag tagcggccgg tctcgtcccc ccagaccc
//
LOCUS       DAOOKS010000631         1178 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_161723_length_1178_cov_2.947462, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000631 DAOOKS010000000
VERSION     DAOOKS010000631.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161723_length_1178_cov_2.947462"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="PK112_09145"
     CDS             <1..691
                     /locus_tag="PK112_09145"
                     /inference="COORDINATES: protein motif:HMM:NF026048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPC12247.1"
                     /translation="SEMLGDFSKRFLRLRIRPWKVLDWPLRMIKYLLLGFFVWAILIQ
                     MTPASIEAFVYSNYNQVADILMLRFFTDISRFALAVTGALVLLSLVVRGFWCRYLCPY
                     GALLGLIGLISPTRIRRNETTCIDCARCAKACPAFIKVDKVKYVVSDECVGCMACVDS
                     CPVKDALTVHMFTKKRPVNKRYWALALVVLFWVGLAGFKLFGPWENNISDAQYREHLP
                     RLERGEYVHPR"
     gene            738..>1178
                     /locus_tag="PK112_09150"
     CDS             738..>1178
                     /locus_tag="PK112_09150"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPC12248.1"
                     /translation="MNESFPSVFEVYAHEYDLITNAAQREPHHAAEVAAMIARFAPAS
                     VLDAGCATGLTTLLFARQGVAAVGLDRSPRILAVARQTRCPEGLPISYRRGTFEDLPK
                     SLRNRFDLVVCLANAIAGVGTVARLNLALRNFSAALRPGGHLVLQ"
BASE COUNT          181 a          408 c          343 g          246 t
ORIGIN      
        1 ctcggaaatg ctgggcgatt tctcgaaacg attcctgcgt ctgagaatcc gcccctggaa
       61 agtgctcgac tggccgctcc ggatgatcaa gtacctcctc ctcggtttct tcgtctgggc
      121 gatcctcatc cagatgaccc cggcgtcgat cgaggcgttc gtctactcga actacaacca
      181 ggtcgccgat attctcatgc tccgcttctt caccgacatc tcccgcttcg ccctcgccgt
      241 gaccggcgcc ctggttttgc tgtcgctggt cgtgcgcggc ttctggtgcc gctacctgtg
      301 tccctacggc gctcttctcg gcctgatcgg attgatctcg cccacccgca tccggcggaa
      361 cgagacgacc tgtatcgact gcgcccgctg cgccaaggcc tgcccggcct tcatcaaggt
      421 tgacaaggta aaatacgtgg tctccgatga gtgcgtgggc tgcatggcct gcgtcgacag
      481 ctgcccggtg aaggatgccc tgacagtgca catgttcacg aaaaaacggc ccgtcaacaa
      541 gcgctactgg gcgcttgccc tggtcgtctt gttctgggtc gggctggccg ggttcaagct
      601 gttcggaccc tgggaaaaca acatcagcga cgcccagtac cgcgagcacc tgccgcgcct
      661 cgagcgggga gaatacgtcc acccgcgtta ggccgacacg cttgcggggc ggggacaatc
      721 accgtatcat ccctcccatg aatgagtcat tcccgtctgt ttttgaggtc tatgcccacg
      781 agtacgacct catcaccaac gcggcccagc gcgagccgca ccacgccgcc gaagtggcgg
      841 cgatgatcgc ccggttcgcg ccggcctcgg ttctggatgc cggctgcgcc accggtctca
      901 cgaccctcct gtttgcccgg cagggggtgg ctgcggttgg tcttgaccgc tctccccgga
      961 tcctcgccgt agcccggcag acccgctgtc cggagggact gccgatctcg taccgccggg
     1021 gtactttcga ggatctgccg aaatcgctgc gcaaccgctt tgatctggtc gtgtgtttgg
     1081 ccaacgccat cgcaggagtc ggcaccgtcg cccgcttgaa cctggccctg cggaactttt
     1141 ccgccgccct ccggcccggc ggtcacctcg tgctccag
//
LOCUS       DAOOKS010000632         1160 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_165683_length_1160_cov_3.627149, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000632 DAOOKS010000000
VERSION     DAOOKS010000632.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_165683_length_1160_cov_3.627149"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="PK112_09155"
     CDS             complement(<1..424)
                     /locus_tag="PK112_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12249.1"
                     /translation="MSIFALWSNLSGNLFNSEEEVVSVPTDGGYIPATRSTTETAFAL
                     HLGVQLNPPAARSPIRPRVALGAGLWMFNSDIAYRVDREDDPFLSDDETSFEFGLRGN
                     GGIDVFFAEKWGIALDFTFDRLFGVDRVVPTDDAGGGRP"
     gene            complement(679..>1160)
                     /locus_tag="PK112_09160"
     CDS             complement(679..>1160)
                     /locus_tag="PK112_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12250.1"
                     /translation="FRYPFLHYGTSLEMYRAVAGYLDANDIAVAHASVIVDDYLYDLG
                     LQKVGGRPTDKEYEQLMNEYVNHVLDQIEAAEVLAKEILGRPCRQILQLRANQLNAMY
                     LDVMLQAIKDMGYEFISLDRALQDEAYQKPHAYFGGKGVGYLEMIDRSDPDLLPAGE"
BASE COUNT          199 a          369 c          393 g          199 t
ORIGIN      
        1 ccgggcggcc gcccccggca tcgtcggttg gaaccacccg gtccaccccg aacaggcggt
       61 caaaggtgaa atcgagcgca attccccact tctcggcgaa gaacacgtcg atcccgccgt
      121 tgccgcggag accgaattcg aaactggtct cgtcatcgga caggaacggg tcgtcctcgc
      181 ggtcgacgcg gtaggcgatg tcgctgttga acatccacag gcccgccccc aacgcgactc
      241 tcgggcggat cgggctgcgg gcggcgggcg gattgagctg gacgcccagg tggagggcga
      301 acgcagtctc ggtggtcgag cgggtggccg gtatgtaccc gccgtcggtc ggcacactca
      361 cgacctcctc ctcgctgttg aaaaggttgc cgctgagatt ggaccagagg gcgaagatgc
      421 tcatcggttt cgggcgcaaa gtgacccggc cgagcacgct gaaggcggag accgtagcgg
      481 gacacatccc cctcggccca cgtgatgccg gcgctgagag ccgcctctcc ggggcggtca
      541 agcggatcgg cgccggccgg ggcggccggc agcgtcgcca acagaaccaa caggaatgac
      601 cttgccctgc gcataaaacc tcctcccgtc cgcgcggcgc gggccggctg tacccaacct
      661 cccgcgccgc agtgactgtc actcgcccgc cgggagcaag tccgggtcgc ttcggtcgat
      721 catctcgaga tacccgaccc ccttgccgcc gaaatacgcg tgtggcttct ggtaggcctc
      781 gtcctggagc gcccggtcga gggaaatgaa ctcgtagccc atgtccttga tcgcctggag
      841 catgacgtcg aggtacatcg cgttgagctg gttcgcgcgc aactggagga tctggcggca
      901 gggccggccg agtatctcct tggccaacac ctccgcggct tcgatctgat cgaggacatg
      961 gttgacgtat tcgttcatga gctgttcgta ctctttgtcg gtcggccggc cgccgacttt
     1021 ctgcagcccg aggtcgtaga ggtagtcatc gacgatgacg ctcgcgtggg cgaccgcgat
     1081 gtcgttggcg tcgaggtacc cggcgacagc gcggtacatt tcgagggagg tgccgtagtg
     1141 gaggaagggg tagcggaaat
//
LOCUS       DAOOKS010000633         1159 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_165894_length_1159_cov_3.777174, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000633 DAOOKS010000000
VERSION     DAOOKS010000633.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_165894_length_1159_cov_3.777174"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /gene="bamD"
                     /locus_tag="PK112_09165"
     CDS             <1..715
                     /gene="bamD"
                     /locus_tag="PK112_09165"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HPC12251.1"
                     /translation="QYYLGLAYYGNEDFELAALEFDRLARNYPASAFFANAIFMRGVA
                     YFDSTPKNPGLDQTELEKAIAVFEDFLIDFPESPLVPDVQKYLAAARTRFAKKYFNAG
                     IVYERLGAFKAAKVYFQKVIDEYTDTDLAARAAFHRADMDLRLKDFAAARTGFESFLA
                     AFPESDLAGKARKRAVESAYRQCRLSYDKGDTAEARQCLRQFRESYPANGYEDDVNEM
                     LEALDKHPAGSDTTRHAES"
     gene            702..>1159
                     /gene="nadD"
                     /locus_tag="PK112_09170"
     CDS             702..>1159
                     /gene="nadD"
                     /locus_tag="PK112_09170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HPC12252.1"
                     /translation="MRSPEQGGVWGVLGGAFDPVHSGHLALAREILRARNLAGVLFVP
                     AYRHPFKGDRARASWPHRVAMLRLALAGDDRLRVCEIEAEQELSGYTLDTLRALKKAY
                     PQAAFRFLVGADLLDQIDQWHSPEELLRETPVLAGARPGYQLKVSPGLPA"
BASE COUNT          194 a          423 c          347 g          195 t
ORIGIN      
        1 ccagtactac ctcggcctcg cgtactatgg caacgaggac ttcgaactgg cggcgctgga
       61 gttcgaccgc ctcgcgcgca attacccggc ctcggccttt ttcgccaacg ccatcttcat
      121 gcgcggcgtc gcctatttcg actccacccc gaaaaacccc ggtctcgatc agacggagct
      181 tgagaaagcc atcgccgtct tcgaggactt cctgattgat ttccccgaat ccccgctcgt
      241 tcccgatgtg cagaagtacc tcgcggcggc gcgcacgcgg ttcgcgaaga agtatttcaa
      301 cgccggcatc gtctatgagc gcctcggcgc tttcaaggcg gccaaagtgt atttccagaa
      361 agtcatcgac gagtataccg acaccgacct ggccgcccgg gcggcctttc accgcgccga
      421 tatggatctc cgcctcaagg actttgccgc cgcccgcacc ggttttgaaa gcttcctggc
      481 tgccttcccc gaaagcgacc tggcgggcaa agcgcgcaag agggccgtgg aatccgcgta
      541 ccgccagtgc cgcctgagct acgacaaggg cgacaccgcc gaggcgcgcc agtgcctccg
      601 gcagttccgg gagagctacc cggccaacgg gtacgaggat gacgtcaatg agatgctcga
      661 ggcgctcgac aaacaccccg ccgggtccga caccaccaga catgcggagt cctgagcagg
      721 ggggcgtgtg gggcgtcctc ggcggcgcct tcgatccggt gcacagcggg cacctcgccc
      781 tggcccgcga gatcctccgc gcccgcaacc ttgccggcgt cctcttcgtc cccgcctacc
      841 ggcacccgtt caagggtgac cgggcccgcg cctcctggcc ccaccgggtc gccatgctcc
      901 gcctcgctct ggccggggac gaccgcttgc gcgtctgcga gatcgaagcc gagcaggagc
      961 tttccggcta caccctcgac accctgcggg cgctgaagaa ggcgtacccc caggcggcct
     1021 tccgcttcct cgtcggcgcc gacctcctgg accagattga ccaatggcat tcgcctgaag
     1081 agctgcttcg cgagacgccg gttttggccg gcgcccggcc cggctaccaa ttgaaggtca
     1141 gcccggggct gcccgccga
//
LOCUS       DAOOKS010000634         1149 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_168256_length_1149_cov_2.716636, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000634 DAOOKS010000000
VERSION     DAOOKS010000634.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_168256_length_1149_cov_2.716636"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="PK112_09175"
     CDS             complement(<1..400)
                     /locus_tag="PK112_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12253.1"
                     /translation="MSRIHALILTGVLLLAAGAAAQTAPPVLDPIGAQSVNENVSLNF
                     GVSASDADATIPVLSTSALPTGATFTDNLDGTGSFAWTPDFTQASEYSVTFYAADAVT
                     ADIDSEIVSITVNNVNQEPVLAAIGPRSVAE"
     gene            complement(422..>1149)
                     /locus_tag="PK112_09180"
     CDS             complement(422..>1149)
                     /locus_tag="PK112_09180"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HPC12254.1"
                     /translation="QPQPVAPSGGELFATVSLEYHDMFAGSIDELVVSSELPEEIAGV
                     QLNITYDPAAVVLGQPKAADDASGLKLLYKNTGPGEMLVLMHFTRPGDQQQLIQRGLA
                     DLVQIPITAQNDVEYGNPAQLKLTSVKLATPTAKALEVKGFGPTLPAAFTLYQNYPNP
                     FNPMTTIRFELSGTSRRVQLDVFNVLGQQVRRLLDETVAPGEHSVVWDATTDNGSKVA
                     TGVYLYRLQVGDQSDTKKMLLLK"
BASE COUNT          200 a          368 c          345 g          236 t
ORIGIN      
        1 tctcggccac acttcttggc ccgatcgccg ccagcaccgg ctcctggttg acattgttga
       61 cagtaattga cacgatctcc gagtcgatat cggcggtgac cgcatcggcc gcatagaacg
      121 tcacggagta ttcgctggcc tgcgtgaaat ccggggtcca cgcgaaactc ccggtgccgt
      181 cgaggttgtc cgtgaaggtt gcccccgtcg gaagcgccga agtgctcagg acggggatag
      241 tggcatcggc atcgctcgcg gagacgccga aattcagact cacgttctcg ttgaccgact
      301 gggcgccgat cggatcgagc accggcggcg ccgtctgggc cgccgcgccg gcggccagca
      361 ggagaactcc cgtgaggatc agtgcatgta ttctggacat ctaccttctt cctccctgat
      421 gctacttcag caggagcatt ttctttgtat ccgattggtc gcccacctgc aaacgataga
      481 ggtagacgcc cgtagctact ttacttccat tatcggttgt ggcatcccag accacactgt
      541 gttctccggg cgccaccgtc tcgtccagca gccggcgaac ctgctggccg aggacgttga
      601 agacatccag ctgcacccgc cggctggtcc cggacaactc gaaccggatt gtcgtcatcg
      661 ggttgaacgg gttcgggtag ttctggtaga gggtgaacgc cgccggcagt gtcgggccga
      721 accccttgac ttccaaagcc ttagcggtcg gcgtcgccag tttcaccgac gtcagtttca
      781 actgggcggg gttgccgtac tcgacgtcat tctgcgccgt gatcgggatc tgcaccagat
      841 ccgccagacc gcgctgaatg agctgctgct gatcccccgg ccgcgtgaaa tgcatcagca
      901 caagcatttc gccgggtccg gtattcttgt acaggagttt caggcccgag gcgtcgtcgg
      961 ccgccttcgg ctgcccgagc accaccgccg cgggatcgta ggtgatgttc aactggactc
     1021 cggcaatctc ttccggaagc tcggagctga ccaccagctc gtcgatcgag cccgcgaaca
     1081 tgtcgtggta ctccaggctg actgtcgcaa acagctcgcc tccgctcggc gcgaccggct
     1141 gcggctgct
//
LOCUS       DAOOKS010000635         1140 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_170394_length_1140_cov_3.153917, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000635 DAOOKS010000000
VERSION     DAOOKS010000635.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_170394_length_1140_cov_3.153917"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="PK112_09185"
     CDS             <1..325
                     /locus_tag="PK112_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HPC12255.1"
                     /translation="MNIMLATVLERTREIGVRRAVGATRRDIMRQFLIEAVAICLVGC
                     VVGVALGLMLSRAISFYADWPTIVSPYAIVLAVGVSTAVGVIFGLYPAGKAAKLDVIE
                     SLRYE"
     gene            381..>1140
                     /locus_tag="PK112_09190"
     CDS             381..>1140
                     /locus_tag="PK112_09190"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPC12256.1"
                     /translation="MSRYGMRNAALGLAAAGLMAAATPARELTYDAAMEIALRRSSRV
                     EIIRGNLEVAERKYQAEKVNFLLPEISLNGTLPGYSSAEQYTYPDGFGGTKQVGLRKS
                     LDMNTFIGLNQNLITGGSLDIRAYLQGNERHFPQTLRLETGEGVFTANESERTGRFSF
                     ALQQPLLQPSEAKFTLNNRKDELSLARLGRQEETATLKQEVAQAYIGALQLQIDKQIA
                     EEKLRSARLKADIDSVKLQDGILSEEQWLETSSAR"
BASE COUNT          213 a          372 c          377 g          178 t
ORIGIN      
        1 catgaacatc atgctcgcga ctgtgctcga gcggacgcgc gagatcggcg tccgccgggc
       61 ggtgggggcg acccgccgcg acatcatgcg ccagttcctc atcgaggccg tggcgatctg
      121 cctcgtcggg tgcgtggtcg gagtggcgct cgggctgatg ctctcccgcg ccatctcgtt
      181 ctacgccgac tggcccacga tcgtctcccc ctacgcgatc gtgctcgcgg tgggcgtctc
      241 caccgcggtc ggcgtcatct tcggcctcta cccggcgggc aaagcagcta agctcgacgt
      301 gatcgaatcg cttcggtacg aatagccgcc gggggagggc ggacggcaat cggcagccac
      361 agaggagaca ggatgcagat atgagcagat acggaatgcg caacgccgca ctgggcctgg
      421 ccgcggccgg gctgatggcc gccgcgacgc cggcgcgcga actgacctat gacgcggcca
      481 tggagattgc gctgcggcgc tccagccgcg tcgaaatcat ccgcggcaat ctcgaggtgg
      541 cggagaggaa gtaccaggcc gagaaagtga acttcctcct ccccgagatc tccctcaacg
      601 ggacgctccc ggggtactcg tcggcggagc agtacaccta ccccgatggg ttcgggggca
      661 ccaagcaggt ggggctgcgg aagtcgctcg acatgaacac gtttatcgga ctgaaccaga
      721 acctgatcac cggcgggtcg cttgatatcc gggcctacct gcaggggaac gaacgccatt
      781 ttccccagac cctccggctg gaaacgggcg agggggtttt caccgccaac gaatccgagc
      841 gcaccggccg gttctccttt gccctccagc agccgctcct gcagccgtcc gaagcgaaat
      901 tcacgctcaa caaccgcaag gatgagttgt cgctcgcgcg cctgggccgg caggaggaaa
      961 cggcgacgct gaagcaggag gtggcccagg cctacatcgg ggcgctccaa ctgcagatcg
     1021 acaaacagat cgccgaggag aaactccgct cggcccggct caaggcggac atcgactccg
     1081 tgaaacttca ggacgggatt ctctctgagg agcagtggct cgagacctcg tcggcgcggc
//
LOCUS       DAOOKS010000636         1136 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_171373_length_1136_cov_2.969473, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000636 DAOOKS010000000
VERSION     DAOOKS010000636.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_171373_length_1136_cov_2.969473"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="PK112_09195"
     CDS             <1..519
                     /locus_tag="PK112_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPC12257.1"
                     /translation="TPFEADANYLQVEAGTLEDGCNMSSARVKITSSTLSNDFSLTGG
                     QDNGIGAVDIVYYWSGGAMAAYPVSMLTGTAYTANCMVTSGPAKVGPGEQARYSVEIR
                     DRFGNPLGDHTIVLSASGGTVSGISQETNGNGEAGGFVWTAPAAPGDVNLTFTDTDPR
                     GGVILSYKVTVE"
     gene            654..>1136
                     /locus_tag="PK112_09200"
     CDS             654..>1136
                     /locus_tag="PK112_09200"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPC12258.1"
                     /translation="MSDHASDSARADSSLPEAFFASAAAHAERTAFRAEGGRGRAYTY
                     AEAAAAVRRFARELRAQGVTRGTEVGLLAENRPEWGIAHLAILAAGGTVVPIDGTLKP
                     QEMGYLAGHCRLARVVASRAFDKPARELPGVTVIPLEGVWEAGGPSPDPAESGAAAPA
                     D"
BASE COUNT          185 a          378 c          401 g          172 t
ORIGIN      
        1 acgccgtttg aggccgacgc caactacctt caggtcgaag ccggcacgct agaagacggc
       61 tgcaacatgt caagcgcacg ggtcaaaatc acctcgagca ccctctcgaa cgatttctcg
      121 ctcaccgggg ggcaggacaa cggcatcgga gccgtcgaca tcgtgtacta ctggtcgggc
      181 ggggccatgg cggcctaccc ggtctcgatg ttgaccggaa cagcctacac ggccaactgc
      241 atggtcacct cggggccggc aaaagtgggt ccgggggagc aggcccgcta ttccgtggaa
      301 atccgcgatc gattcggcaa cccgctgggg gaccatacga tcgtgctgag cgcctccggc
      361 ggaacggtca gcggaatctc gcaggaaacc aacggaaacg gggaagccgg cggattcgtg
      421 tggaccgccc ccgccgcccc gggtgacgtc aacctgacgt tcaccgacac cgacccgcgg
      481 gggggcgtca tcctctccta caaagtgacc gtcgagtaac cccccggcat ccgccgggag
      541 agtgacatcc ggccgccggg gcgtgcgctc cggcggctgc ctttggggcc tttccggaga
      601 ggccgaccgg acttttccct tgcgcgcttc accgccgatt ctacattggc gacatgtcgg
      661 accacgccag cgacagcgcc cgggctgata gttctcttcc cgaggctttc ttcgcctcgg
      721 cggctgccca tgccgagcgg acggctttcc gggccgaagg gggacggggg agagcgtaca
      781 cctacgcgga agcggccgcg gccgtacgcc ggtttgcccg cgaactccgg gcgcagggcg
      841 tgacccgcgg caccgaggtc gggctgcttg cggagaatcg tcccgagtgg ggaattgcgc
      901 acctggcgat cctggcggcg ggcgggactg ttgtgccgat cgacggaact ctcaagccgc
      961 aggagatggg ttatctggcc ggccactgcc gtctggcgcg ggttgtggcc tcgcgggcct
     1021 ttgacaagcc ggcccgggaa ctgcccggcg tgacggtcat cccgctggag ggcgtgtggg
     1081 aggccggggg cccctccccg gacccggcgg aaagcggggc ggccgcgccg gcggac
//
LOCUS       DAOOKS010000637         1133 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_172115_length_1133_cov_2.702226, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000637 DAOOKS010000000
VERSION     DAOOKS010000637.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_172115_length_1133_cov_2.702226"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            135..222
                     /locus_tag="PK112_09205"
     tRNA            135..222
                     /locus_tag="PK112_09205"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:169..171,aa:Ser,seq:cga)
     gene            complement(398..>1133)
                     /locus_tag="PK112_09210"
     CDS             complement(398..>1133)
                     /locus_tag="PK112_09210"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPC12259.1"
                     /translation="DGGVTVGDATMLICIIFRDCQTPLACGVVKSARRAAPEPIASLR
                     TEFDGAATTVILEASEPLMGVQITLQADHPDSIANTTETGLELVHGTVDGLTRVGLLD
                     LQGEVHLPQGSTTLFTVPGEVTLVDGLVADMSHQSMIARIGGKSSSPNLPRRFELAQN
                     YPNPFNPTTVIQFALPVATHVRLEVINILGQTVTTLVDRQMEAGYQSVEWNSTNSDGS
                     RVASGVYFYRLEAGTFRDTKKMLLLK"
BASE COUNT          204 a          344 c          378 g          207 t
ORIGIN      
        1 ccgggtggcc tgagccggga tgcggctgct ctgggccgag gcgggcggaa attggacttt
       61 acaaacggtg gcggaagatt aaattggata gtcgcggcga ccggggcttt cggccccggc
      121 gcctgcgaag acacggagag gtggctgagt ggtcgaaagc ggcggtctcg aaaaccgttg
      181 taccctcggg taccctgggt tcgaatccca gcctctccgc cattttgtgt gagggcgcgg
      241 aggttgcggc gggggaaagc gggaggtcca gtgcgcattc aggcaatttg accgggagaa
      301 ggcggcaaac ttccaccgta ttgtcgccac cggccggcaa atgagaaaag ggcggccggc
      361 ggccgcccct ttcgtttgcc gtttccgtgt ccagcagcta cttcaacaag agcatcttct
      421 tcgtgtcgcg gaatgtcccc gcttccaggc ggtagaaata cacgccgctc gcgacccggc
      481 tcccgtcgga gttggtgctg ttccactcga ccgattgata ccccgcctcc atctggcggt
      541 caaccagcgt cgtcaccgtc tggccgagaa tgttgatcac ctcgagccgg acgtgggtcg
      601 ccaccgggag cgcaaactgg atcacggtcg tggggttgaa cgggttcggg tagttctgcg
      661 ccagctcaaa ccgccgcggc agattcggcg aactggactt gccgccgatc cgggcgatca
      721 tcgactggtg cgacatatcg gccacgaggc catcgaccag cgtcacctcg cccggaaccg
      781 tgaacagcgt cgtcgatccc tgcggcaggt gaacctcgcc ttggagatcg agcaggccga
      841 cacgagtgag cccatcgacc gtgccgtgaa ccagctcaag gccggtttcg gtggtgttgg
      901 cgatgctgtc cgggtgatcc gcctgcagtg tgatctgcac gcccatgagc ggctcggagg
      961 cttcgagaat gaccgtcgtc gcggcgccat cgaactcggt gcgcagcgag gcgatcggtt
     1021 ccggggccgc ccggcgggcc gacttgacaa ccccgcaggc caagggagtc tggcagtcgc
     1081 ggaatattat gcatatgagc atggttgcat cgcccactgt aaccccacca tca
//
LOCUS       DAOOKS010000638         1129 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_173097_length_1129_cov_3.094972, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000638 DAOOKS010000000
VERSION     DAOOKS010000638.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1129
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_173097_length_1129_cov_3.094972"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="PK112_09215"
     CDS             <1..492
                     /locus_tag="PK112_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12260.1"
                     /translation="AKQARDRLEKNRKRNARNIAYPMLRPFRPENRKDYEIAYVGLDE
                     VEDTTCYHFRVTPKVKTDEHIAGDYYFETDGFHLVRVDFSPARLVKKAMFKLDRLDMS
                     IVYGPAPEGFWLPKQFDVAGRGKAALFFGVNFAGTEYYRNPQINVGQSDTLFKSEDAD
                     NGE"
     gene            482..979
                     /locus_tag="PK112_09220"
     CDS             482..979
                     /locus_tag="PK112_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011822382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HPC12261.1"
                     /translation="MASEKFKTHVVKVEIPEGLNVIFGMAHFIKTAEDLYEALIEASP
                     TIKFGVAFCEASQKRAIRTEGNAGDLIERAAKAAFDVGCGHTFFIYLKDGFPVNVLNR
                     VKSTSEVCRVFCATANPLEVLVAETDLGRGVLGVIDGQPPLGIETEADRAERREFLRR
                     IGYKR"
BASE COUNT          234 a          324 c          381 g          190 t
ORIGIN      
        1 gccaagcagg cgcgcgaccg gttggagaag aaccgcaagc gcaacgcccg caacatcgcc
       61 tatccgatgc tccgcccgtt ccggcccgag aaccggaagg attacgagat cgcttatgtc
      121 ggcctcgacg aagtcgagga cacgacctgc taccattttc gggtgacgcc gaaggtcaag
      181 acggacgagc acatcgccgg ggactactat ttcgagaccg acgggtttca cctggtgcgg
      241 gtggatttct ccccggcccg gctggtgaag aaagcgatgt tcaagctgga tcgtctggac
      301 atgtcgatcg tctatggtcc ggcgccggag ggattctggc tgccgaagca gttcgacgtg
      361 gcggggcggg gcaaagcggc cctgtttttc ggagtcaatt tcgccggcac tgaatactac
      421 cggaatcccc agatcaacgt cggccagtcc gacacgctgt tcaaatcgga ggacgcggac
      481 aatggcgagt gagaagttca agacccacgt ggtgaaggtc gagatcccgg agggactcaa
      541 cgtgattttc gggatggcgc acttcatcaa gacagccgag gatctctacg aagcgctgat
      601 cgaggcctcg cccacgatca aattcggggt ggccttctgc gaggcgtccc agaagcgggc
      661 gatccggacc gaggggaacg cgggggacct gatcgagcgg gcggcgaagg cggcgttcga
      721 cgtcggctgc ggccacacct ttttcattta cctcaaggac ggtttcccgg tcaacgtgct
      781 caaccgggtg aagagcacca gcgaagtgtg ccgggtcttc tgtgcgaccg ccaacccgct
      841 cgaagtgctg gtggccgaga ccgatctcgg gcgcggcgtg ctcggggtga tcgacgggca
      901 gcccccgctg gggatcgaaa cggaagcaga cagggctgag cggcgggagt ttctccgccg
      961 gatcggctac aaacggtaac ggcggccccg ttccgggcca cggcgggcgg gcggacagga
     1021 cgacaaacat gcgattcgag ctcgataaga atgcgaccga acgggtggtg atcgaagaca
     1081 gcgagtacaa gggacaccac ctggtgcagc tccgtattta cttcctggc
//
LOCUS       DAOOKS010000639         1125 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_174221_length_1125_cov_1.894393, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000639 DAOOKS010000000
VERSION     DAOOKS010000639.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_174221_length_1125_cov_1.894393"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..961
                     /gene="dctP"
                     /locus_tag="PK112_09225"
     CDS             <1..961
                     /gene="dctP"
                     /locus_tag="PK112_09225"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HPC12262.1"
                     /translation="GADTIRLKLATLVPTGSSYHKSLLVLRESWRKLSNGSVDLVIYA
                     DGKLGSEADTVGLMKLNSIQASMITVAGLSEIEKSVGSLQNIPMGFRDFSEVDYVGEK
                     LQPMLEEILDQKGFRVLFWSDAGWVHFFAKKAVVRPDDLKKLKLFCWAGNPEQVAIYK
                     SSGMDAVHLETADIVPGLQTGLIDATPAPPVFALKAQIDSRAGHMLQLNWAPLVGACV
                     VRKETWEKIPAALREKLQQAAIDIGREIKSNGRKEMVESIAEMEKRGLKVTKVTPELE
                     AEWRAVAEAAYPQIRGRIVPTEMFDRALELIREYRKSKSAPAQ"
BASE COUNT          222 a          364 c          360 g          179 t
ORIGIN      
        1 cggagccgac accataaggc tcaagctcgc caccctggtg ccgaccggca gttcctacca
       61 caagagcctc ctggtcttgc gcgagtcctg gcggaaactc tccaacggca gcgtcgatct
      121 cgtcatctac gccgatggca aactgggaag cgaggcggac accgtcggcc tcatgaagct
      181 caactcgatc caggcctcga tgatcaccgt cgccgggctg tccgagatcg agaagagcgt
      241 cggaagcctg cagaacatcc cgatgggctt ccgcgacttc agcgaggtgg attacgtggg
      301 ggagaagttg cagccgatgc tcgaagagat cctggaccag aaaggcttcc gggtcctgtt
      361 ctggtccgac gccggatggg ttcatttctt cgccaagaaa gccgtggtcc gcccggacga
      421 cctcaagaag ctcaagttgt tttgctgggc cggaaacccc gagcaggtcg ccatctacaa
      481 gtcgtccggc atggacgccg tccatctgga gacggcggac atcgtgcccg gtctgcagac
      541 gggcctgatc gatgccacgc cggcgccgcc ggtgttcgcc ctcaaggccc agatcgactc
      601 ccgcgccggc cacatgctgc agctcaactg ggcgcccctc gtcggcgcct gcgtcgtccg
      661 caaggaaacc tgggagaaga tccccgccgc gctccgcgag aagctccagc aggccgcgat
      721 cgacatcggc cgggaaatca aaagcaacgg ccgcaaggag atggtcgagt ccatcgccga
      781 aatggagaag cgcgggctca aggtcaccaa ggtcacgcct gagctggagg ccgagtggcg
      841 ggccgtggcc gaagccgcct atccccagat ccgaggtcgc attgtcccga cggagatgtt
      901 cgaccgggcc cttgaactga ttcgggaata ccggaaatcg aagtccgcac cggcccagta
      961 gcagcggcga gatgactcgc gctccgagac gcagccgcgc ggtgcatcca attgtgacgt
     1021 tgagtacagc ccggacattg cgcgctcgct tcgggtagtg tccggtcgca ggaccgcttg
     1081 gggccgcggc tgtgggctaa agccatcccg cgcggtacca ggcca
//
LOCUS       DAOOKS010000640         1119 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_175550_length_1119_cov_2.968985, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000640 DAOOKS010000000
VERSION     DAOOKS010000640.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_175550_length_1119_cov_2.968985"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="PK112_09230"
     CDS             complement(<1..183)
                     /locus_tag="PK112_09230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPC12263.1"
                     /translation="MIRREGGRPGFTLVEILVVVSIIVIMFGFAAPVIARFMSGKKVK
                     AVTGRLARGLLSARMKA"
     gene            complement(180..899)
                     /locus_tag="PK112_09235"
     CDS             complement(180..899)
                     /locus_tag="PK112_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HPC12264.1"
                     /translation="MTISATGDRGGFTLLEILVVISLMTFLVTALVALTLNLSHRAYE
                     ASTRTLLDRIDLSIDKYKDLTGFCPPDGFDAPVTTREGGLEIRSAACLYEFLGRPLQV
                     IKQGAGGKVVVERYDSPVMRGLKESELVRVPGDSADVAEITDAWGGPLHYDRLEGDDS
                     YSPQNTPDVHLVPPEYHGPDPRDVPVVAVAEAGTGQNPGRYDVWSHGRHGHEAPEREE
                     DAAALLRETIGNWQPPGKKGP"
     gene            complement(877..>1119)
                     /locus_tag="PK112_09240"
     CDS             complement(877..>1119)
                     /locus_tag="PK112_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12265.1"
                     /translation="MDGDRTRPLTAEERADPDTEIVVVDPWGEIYIARENESKQRKEI
                     WMRNRDGMDLYSKGKNAEDDTVLGTEGKGNDDIGNW"
BASE COUNT          184 a          397 c          332 g          206 t
ORIGIN      
        1 ggccttcatg cgggccgaga gcagaccgcg cgccagcctg cccgttacgg ccttcacctt
       61 cttgccggac atgaacctgg cgatcaccgg cgccgcaaac ccaaacatga tcacgataat
      121 cgacacgacg acgagaatct cgaccagcgt gaatccggga cggcctcctt ctcggcggat
      181 catggtccct tcttccccgg cggctgccag ttgccgatgg tttcccggag caaggccgcc
      241 gcatcttcct cgcgctccgg ggcctcgtgt ccatggcggc cgtgcgacca cacatcgtag
      301 cgccctggat tctgccctgt ccccgcctcc gcgaccgcga cgacgggcac atcgcgcggg
      361 tcgggcccgt ggtattcggg cggcaccaga tgcacatccg gcgtgttttg cggactatag
      421 gaatcatccc cctcgagccg gtcatagtgc agcggccccc cccatgcatc ggtgatctcg
      481 gccacatcgg cgctgtcgcc ggggacgcgc accagctccg actccttgag cccccgcatc
      541 accggggaat cgtagcgctc gacgacgacc ttgccgcccg cgccctgctt gatgacctgg
      601 agcgggcggc cgagaaactc gtacaggcac gccgcggagc ggatctcgag gccgccctcc
      661 cgcgtcgtca ccggcgcatc gaatccgtcg ggggggcaga accccgtcag atccttgtac
      721 ttatcgatgg aaagatcgat gcgatcgagc agcgtccgcg tggaagcctc gtaggcgcgg
      781 tggctcagat tcagcgtcaa cgccaccagc gccgtcacta aaaacgtcat caaggagatg
      841 acgaccagga tctccagcag ggtgaagccc ccgcggtcac cagttgccga tatcgtcatt
      901 gcccttaccc tcggtgccca gcaccgtgtc gtcctcggcg tttttgccct tggagtacag
      961 gtccattcca tcgcggttgc gcatccagat ctccttgcgc tgcttggact cgttctcccg
     1021 ggcgatgtag atctcacccc acgggtcgac gacgacgatc tcggtgtccg gatcggcgcg
     1081 ctcttcggcg gtgagcggcc tggttcgatc tccgtccat
//
LOCUS       DAOOKS010000641         1116 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_176391_length_1116_cov_2.112158, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000641 DAOOKS010000000
VERSION     DAOOKS010000641.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_176391_length_1116_cov_2.112158"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="PK112_09245"
     CDS             <1..581
                     /locus_tag="PK112_09245"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HPC12266.1"
                     /translation="PNLALFLGVVGLLLLYFEFTHSGFVAPGVIGGICVLLSVLGFSF
                     LPINYVGVLLILLAIGLLIAEVKVGGFGVLGIGGVVAMVFGMLILIDSPDPAMRVGLF
                     TALSLALPFAAIFLIILIALIRSYRQKAETGNEGMVGMVGVADSEIGEAGRIRIRGEY
                     WAARSDAPVPAGKSVRVRAVENLTLLVEEVKK"
     gene            606..1116
                     /locus_tag="PK112_09250"
                     /pseudo
     CDS             606..1116
                     /locus_tag="PK112_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683266.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
BASE COUNT          178 a          335 c          374 g          229 t
ORIGIN      
        1 agccgaacct ggcgctgttt ctcggggtcg tggggttgct gctgctctac ttcgaattca
       61 cccactcggg cttcgtggcc ccgggcgtca tcggcgggat ctgcgtgctg ctgtcggtcc
      121 tcggcttctc gttccttccg atcaactatg tgggcgtact gctgattctg ctcgccatcg
      181 ggctgctgat cgccgaagtc aaggtcgggg ggttcggagt gctcgggata ggaggcgtgg
      241 tcgctatggt attcgggatg ctcattctca tcgactcgcc ggaccccgcc atgcgggtcg
      301 ggctgttcac ggctctgtcg ctggccctgc cgttcgcggc gatctttctc atcatcctga
      361 tcgccctgat ccgttcctac cggcagaagg ccgagacggg aaacgagggc atggtcggca
      421 tggtcggcgt ggccgacagc gagatcggcg aggccggccg gatccggatc cgcggggagt
      481 attgggccgc gcgctccgac gcgccggtac cggccgggaa atccgtccgg gtgcgcgcgg
      541 tggaaaacct gacgttgctg gtggaagagg tgaagaaata acatctttgt tcacgggagg
      601 cgcgtatgtc accggtctgg tttggagtca acatcgttct gctttatctc tggagcacga
      661 tcagcattct ccgggagtac gagcgcggcg tgattttccg cctcgggcgc ctggtgccca
      721 acgccgccgg tccggggctg attttcgtct tctggccggt ctataaaatg gtgcgcgtca
      781 gcctgcggac cgtcacgctc gacgtgccgc cgcaggacat catcacgcgc gacaacgtgt
      841 cggtgaaggt caacgccatc gtgtacttcc gggtgatgaa cgcgccccgg gcgatcgtgg
      901 aggtggagaa ctacctctat gcgacctcgc agctggccca gacgacgctg cgctccgtcc
      961 tgggaagtcg acctggacga cctgctcagc cgccgcgaga agctgaacgt caagctgcag
     1021 gaaatcctgg accagcacac ggacatctgg ggcatcaagg tgagcctcgt cgaagtcaag
     1081 caggtcgacc tgccccagga gatgcagcgc gccgtg
//
LOCUS       DAOOKS010000642         1115 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_176531_length_1115_cov_3.278302, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000642 DAOOKS010000000
VERSION     DAOOKS010000642.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_176531_length_1115_cov_3.278302"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="PK112_09255"
     CDS             <1..340
                     /locus_tag="PK112_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12267.1"
                     /translation="CRMCLACDDVLTEVHGANRTRVNRDYLIAGALLADVGKLMEYEI
                     VDGKPVKSDYGKHLRHPFSGVGLAFRHGLPSEVMHVIATHSKEGEGEKRLPESIIFHH
                     CDFIDFDLVK"
     gene            414..>1115
                     /locus_tag="PK112_09260"
     CDS             414..>1115
                     /locus_tag="PK112_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HPC12268.1"
                     /translation="MSHSQTLVEKIAQRYAVGLEPGHAVRAGDYLSIRPAHVMTHDNT
                     GAVIPKFQSIGAARVANPRQPVFTLDHNVQDTTESNLAKYRKIESFAAAHGIDFYPAG
                     RGIGHQIMCEEGYAWPGTMVVASDSHSNMYGGLGCLGTPIVRTDAAAIWATGRTWWQV
                     PPVARCQLKGQLPPGATGKDVIITLCGYFNKDEVLNHAVEFAGDGLRSLSVDERLAIA
                     NMTTEWGALVGLFPID"
BASE COUNT          210 a          354 c          370 g          181 t
ORIGIN      
        1 ctgccgcatg tgtctggcct gcgacgacgt gctgaccgaa gtgcacgggg ccaaccgcac
       61 cagggttaac cgcgattacc tcatcgcggg ggcgctcctg gcggacgtgg gcaagctgat
      121 ggagtacgag attgtcgacg gcaagccggt gaagagcgac tacggcaagc acctccggca
      181 ccccttctcc ggggtcggac tggcctttcg gcacgggctg ccctcggaag tgatgcacgt
      241 catcgcgacg cacagcaagg agggggaggg tgaaaagcgg ctgccggaga gcatcatttt
      301 ccaccattgt gattttatcg attttgacct ggtgaaataa gcgtgggggg gagccgcggg
      361 gctcccgcct gccgaacggg aaaacgaagg cccacgtcat aaaggatcgc cccatgtccc
      421 attcgcagac tctcgttgag aagatcgccc agcgctacgc ggtcgggctc gagcccggtc
      481 acgcggtccg cgcgggcgac tacctctcga taaggccggc ccacgtcatg acgcacgaca
      541 acacgggcgc cgtgataccg aaattccagt cgatcggcgc ggcccgggtg gccaacccgc
      601 gccagccggt gttcacgctc gaccacaacg tccaggacac taccgaatcg aacctcgcca
      661 agtaccgcaa gatcgagtcg ttcgcagcgg cgcacgggat cgatttctac ccggcgggac
      721 gggggatcgg ccaccagatc atgtgcgagg aaggatacgc ctggccgggg accatggtgg
      781 tggcgtccga ttcgcactcg aacatgtacg gcgggctggg gtgtctcggc acgccgatcg
      841 tccgcaccga cgcggccgct atctgggcca ccggtcgcac ctggtggcag gtgccgccgg
      901 tcgcccgctg tcaactcaag ggacaacttc cgccgggtgc gaccggcaag gatgtgatca
      961 tcacgctctg cggctatttc aacaaggacg aggtgctcaa ccacgcggtc gaatttgccg
     1021 gcgacgggct ccgctcgctg tcggtcgatg agcggctggc gatcgccaac atgacgaccg
     1081 aatggggggc gctggtcggc ctcttcccca tcgac
//
LOCUS       DAOOKS010000643         1113 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_177052_length_1113_cov_2.793951, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000643 DAOOKS010000000
VERSION     DAOOKS010000643.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_177052_length_1113_cov_2.793951"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(382..930)
                     /gene="folE"
                     /locus_tag="PK112_09265"
     CDS             complement(382..930)
                     /gene="folE"
                     /locus_tag="PK112_09265"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016493613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HPC12269.1"
                     /translation="MLMEDLYRQLMIQIGEDPNREGLRNTPRRAARAFEFLTRGYKED
                     IDAIVNNALFEADTKEMILVRNIEMYSMCEHHLLPFIGKCHVGYIPNRKVLGLSKVAR
                     IVDVYARRLQIQEKLTKEIGEAIQKYTNALGVAVVIEAQHLCMMMRGVEKQNSVMTTS
                     YIIGEFHEDNATRAEFFSLINK"
BASE COUNT          203 a          404 c          287 g          219 t
ORIGIN      
        1 ggagtgatat ccatgaaact caagaacgaa gagatgcccg aacgtcgcaa agacaatcgg
       61 aagcacgagc aggaagctat cgattcgcgc gaccacgaga aacagcgcga gaccgcggcg
      121 aggaaaactg ccccgaaggc aaggaagtcg aagaaatccg gcggttcctg acaacccgac
      181 cgcaacatgc cgtcgcgagg cgatgccgct gacccatggc gccggccgcc gcccggttcc
      241 gctgcgccgg ccagtccttc tgccaccagc ggtacgacag ggagatctcg aatttcttgc
      301 tgatcgtcag atacatgcgc gtcctttccg ccgccgtcgt ttttcccccg cccctccggt
      361 cgcccccggc ccgcccggcc cttacttgtt gatcaggctg aaaaactccg cccgggtcgc
      421 attatcctca tggaactccc ctatgatata cgacgttgtc atcaccgagt tttgcttctc
      481 caccccccgc atcatcatgc acaggtgctg cgcctcgatc accaccgcca cccccagcgc
      541 gtttgtgtac ttctggatcg cttccccgat ctccttggtc agtttctcct ggatctgcag
      601 ccgccgcgcg tagacatcca caatccgcgc caccttcgac agccccaaca ccttccggtt
      661 cgggatatac ccgacgtggc atttcccgat aaacgggagc aggtggtgtt cgcacatcga
      721 gtacatctcg atattccgca cgagaatcat ctctttcgtg tccgcctcga acagggcgtt
      781 gttcacgatc gcgtcgatgt cctctttgta gccccgcgtg agaaactcga atgcccgcgc
      841 cgcgcgccgc ggtgtgttgc gaagcccctc ccggttcgga tcttcgccga tctggatcat
      901 caactgtcgg tacagatctt ccatcagcat acgggtcacc tgtccttccc tgagttgttt
      961 gatcggcgtt ctctccggcc ctgccgccgc cgcgggccgc ccctcccggt aggctccgtc
     1021 cgcccccgac cgccgggacc ggttcgccgg tccgcaccgc cgtagtatac ctgccctccg
     1081 cccccgacac aaacgcaaaa gcgccccgcg gcg
//
LOCUS       DAOOKS010000644         1111 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_177521_length_1111_cov_3.896780, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000644 DAOOKS010000000
VERSION     DAOOKS010000644.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_177521_length_1111_cov_3.896780"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1111)
                     /locus_tag="PK112_09270"
     CDS             complement(<1..>1111)
                     /locus_tag="PK112_09270"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HPC12270.1"
                     /translation="PEIESRFGSPIRSVVVVPLIGRGHMIGVVEALNKVDGVFTPADL
                     DILVGLANQIAVAIDNAHLYRVARREALEKELLFEIGKKLSSSISQKEVMREILHSVK
                     RAIDFDAGGVFVIEPAKDIIGEVYTEGYDDPGSEERVLVKTGEGLIGHVTATGEPVIV
                     PDVSVDPRYMSARETTRSEIVVPMLLNNRVVGVLNLESDTPGAYRVDNLSLLQAFAAQ
                     AAVSLDRARLHEEILAAKRLEEQLKIAREIQQTFLPEDDPTIPGYDVSGRNVPSGQVG
                     GDYYDFIHIVDYQTGIAIADVSGKGIPAALIMASFRASLIAEIRNNYSIRTIAAKVNN
                     LVYESVKAGSFVTGVYGVLDSRNHIFTFTNCGHNLP"
BASE COUNT          186 a          300 c          417 g          208 t
ORIGIN      
        1 aggggaggtt gtggccgcag ttggtgaacg tgaaaatgtg gttgcgggag tcgagcacgc
       61 cgtagacgcc ggttacgaaa ctgccggctt tcaccgactc gtagacaagg ttgttgacct
      121 tggcggcgat cgtgcggata gagtagttgt tgcggatctc ggcgatgagc gaggcgcgga
      181 acgaggccat gatgagggcg gccgggatcc ccttgccgga cacgtcggcg atggcgattc
      241 cggtctggta gtcgacgatg tggatgaagt cgtagtagtc gccgccgacc tggccggaag
      301 ggacgttgcg gccggaaacg tcgtagccgg ggatggtggg gtcgtcctcg ggcaggaagg
      361 tctgctggat ttcgcgggcg attttgagct gctcctcgag ccgcttggcg gcgaggattt
      421 cttcgtgcag gcgggcgcgg tcgagggaga cggcggcctg ggcggcaaac gcctggagaa
      481 gcgagaggtt gtcgacccgg taggcgccgg gggtgtcgct ctcgaggttg aggacgccga
      541 cgacgcggtt gttcaggagc atggggacga caatctcgct ccgggtggtc tcgcgggcgc
      601 tcatgtagcg cgggtcgacc gacacatcgg ggacaatcac cggctcgccg gtagcggtca
      661 catggccgat cagcccctcg ccggtcttga cgaggacgcg ctcctccgaa ccgggatcat
      721 cgtaaccctc ggtgtacacc tcgccgatga tgtctttggc cggctcgatg acaaacacgc
      781 cgccggcgtc gaaatcgatc gcccgcttga ccgagtggag aatctcccgc atgacctctt
      841 tctggctgat cgagctggag agttttttcc cgatctcgaa gaggagttcc ttctcgagcg
      901 cttcgcggcg ggcgacgcgg tagaggtggg cgttgtcgat ggccacggcg atctggttgg
      961 ccaggccgac gaggatatcg aggtcggcgg gcgtgaagac gccgtcaacc ttgttgagcg
     1021 cctcgacgac gccgatcatg tggccgcggc cgatcagggg gacgaccacg acggagcgga
     1081 tcggggagcc gaagcggctt tcgatttccg g
//
LOCUS       DAOOKS010000645         1101 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_180152_length_1101_cov_3.456979, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000645 DAOOKS010000000
VERSION     DAOOKS010000645.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_180152_length_1101_cov_3.456979"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..955)
                     /locus_tag="PK112_09275"
     CDS             complement(<1..955)
                     /locus_tag="PK112_09275"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HPC12271.1"
                     /translation="MSRLLDDLNDAQREAVQTTEGPLLIIAGAGSGKTRVLTRRLAYI
                     LTRRLAQPHEILAVTFTNKAAGEMKERVAALLGGAMMDMNVSTFHSFCARFLRREGGA
                     IGYGGDFTIFDAADSETLMKSCIKALNLPVSQFAPKAQLRKVSNAKNRFQSAEGFARS
                     AGGYFKQRTAQLYSLYQARLRECNAMDFDDLLLNTVALLQQHSEIGERYRSRFRYLLV
                     DEYQDTNHVQYLLLKSLVGPHRNICVVGDEDQSIYGWRGADIRNILDFEHDFPGAKVI
                     KLEMNYRSTDVILAAASAVIAHNVARKDKVLRTTRRGGEKIS"
BASE COUNT          195 a          317 c          373 g          216 t
ORIGIN      
        1 ggctgatctt ctcccccccg cggcgcgtgg tgcgcagcac cttgtccttg cgggcgacgt
       61 tgtgggcgat cacggccgag gcggccgcca ggatgacgtc ggtggagcgg tagttcattt
      121 ccagtttgat cactttggcg ccagggaagt cgtgctcgaa atcgaggatg ttgcggatat
      181 cggcgccgcg ccagccgtag atcgactggt cctcatcgcc gaccacgcag atgttccggt
      241 gcggcccgac gaggcttttc agcaggaggt actgcacgtg gttggtgtcc tggtactcgt
      301 ccaccaggag atagcggaag cgggaccggt agcgctcgcc gatttcgctg tgctgctgga
      361 ggagggcgac ggtgttgagg aggaggtcgt cgaaatccat ggcgttgcac tcgcgcagcc
      421 gcgcctggta gagggagtac agttgggcgg tgcgctgttt gaagtagccg ccggcgctgc
      481 gggcgaaacc ctcggccgac tggaaccggt tcttggcgtt cgagaccttg cgcaactggg
      541 ctttcggcgc gaactgcgag accggcaggt tgagcgcttt gatgcagctc ttcatgagcg
      601 tctcggaatc ggcggcgtcg aaaatcgtga aatcccctcc gtagccgatc gccccgccct
      661 cacgccggag gaaacgggcg cagaacgagt ggaaggtcga gacgttcatg tccatcatgg
      721 cccccccgag cagggcggcc acccgttctt tcatctctcc ggccgccttg ttggtaaaag
      781 taacagcaag gatttcgtgc ggctgcgcca gtcgccgggt caggatatag gcaagccggc
      841 gggtcaggac ccgggtcttg ccggaaccgg ccccggcgat gatgagcagc gggccctcgg
      901 tcgtctgcac cgcctcccgc tgcgcgtcgt tgaggtcatc gagaaggcga ctcataccga
      961 gcgaatatag gttcgcccgg ggtcggcgca atcggatttc acgatttttc tgtggccggg
     1021 cgcaacggcg aaagacatat ttatgacagg acagtcacac ttcctggatc aggagacgaa
     1081 agcatgaacc taattcgctt g
//
LOCUS       DAOOKS010000646         1085 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_184331_length_1085_cov_3.434951, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000646 DAOOKS010000000
VERSION     DAOOKS010000646.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_184331_length_1085_cov_3.434951"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /gene="liaF"
                     /locus_tag="PK112_09280"
     CDS             <1..535
                     /gene="liaF"
                     /locus_tag="PK112_09280"
                     /inference="COORDINATES: protein motif:HMM:NF021443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HPC12272.1"
                     /translation="PSPAGPAPQTFWAHEGAQSTAAPAGDAPAGGSADPAAPAGGPQF
                     QSPEYGVMRDGKLRYSKLIGDLTVDLRDRAVQHVEVSMGIGDLEIRVHGARLEGGLCR
                     MIVSGFVGDIRILLPPDMAVLAHCSNFIGDIDLLGRRVSGFSSNLDAQTANYAAAERR
                     LYIAANSFLGDIKVYQV"
     gene            complement(560..>1085)
                     /locus_tag="PK112_09285"
     CDS             complement(560..>1085)
                     /locus_tag="PK112_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013681468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPC12273.1"
                     /translation="AGPDVSREGVQRDILPIVEGSAVMTKYGMVRTDHILFIAAGAFH
                     SARPSDLIPELQGRFPIRVELDSLTADDFERILTEPKSALVLQYQALLKTEGIRLEFQ
                     KEAVRRIAEFAERVNSEAENIGARRLQTVMTTLLEEILFEPPEKKRVVITARMVEKTL
                     SAIIENEDLSRYIL"
BASE COUNT          176 a          337 c          368 g          204 t
ORIGIN      
        1 gccctctccg gccggaccgg ctccgcagac tttctgggcg catgaggggg cgcaaagcac
       61 ggcggccccg gccggcgacg cacccgcagg aggatcggcc gatccggcgg ccccggccgg
      121 cggaccgcag ttccaatccc ccgagtacgg tgtcatgcgg gacgggaagc tccgctacag
      181 caagctgatc ggcgatctga ccgtcgatct gcgggaccgc gccgtgcagc acgtcgaggt
      241 ttcgatggga atcggcgatc tggaaatccg tgtccacggg gccagactgg agggggggtt
      301 gtgccggatg atcgtctccg ggtttgtcgg cgatatacgg atcctgttgc cgcccgacat
      361 ggcggtcctc gcgcactgct cgaatttcat cggcgacatc gatttgctcg ggcgccgggt
      421 ctcgggtttc agcagcaatc tcgacgcgca gaccgccaac tacgccgccg ccgaacgccg
      481 gctgtacatc gccgccaaca gcttcctcgg ggacatcaag gtctaccagg tgtaggaagc
      541 cgcccggcgc tcctttccgc tacaggatat accggctcag gtcttcgttt tcgataatgg
      601 ccgagagggt cttctcgacc atcctcgcgg taatcacgac tctcttcttt tccggcggtt
      661 cgaagaggat ttcttcgagc agcgtggtca tgaccgtctg caggcggcgg gcgccgatgt
      721 tttccgcttc gctgttgacc cgctcggcaa actcggcgat gcgccgcacc gcctctttct
      781 gaaactccag ccggatcccc tcggttttca gcagcgcctg gtactggagc acgagcgccg
      841 atttgggttc cgtgaggatg cgctcgaaat catcggcggt cagcgagtcg agttcgaccc
      901 ggatggggaa gcgcccctgg agttcgggga taaggtcgga gggtcgggcc gagtggaagg
      961 cgccggcggc gatgaagaga atgtggtcgg tgcggaccat gccgtacttg gtcatgacgg
     1021 cgctgccctc gacgataggg aggatgtcgc gctgtacgcc ctcgcgggaa acgtcgggac
     1081 cggcg
//
LOCUS       DAOOKS010000647         1075 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_187314_length_1075_cov_1.880392, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000647 DAOOKS010000000
VERSION     DAOOKS010000647.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_187314_length_1075_cov_1.880392"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /locus_tag="PK112_09290"
     CDS             <1..674
                     /locus_tag="PK112_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12274.1"
                     /translation="YVPGVAVRSSPTPGYRSQSLIGGSMFRLLICSGAVLAIAGAVAA
                     AESPIDEGSLYLDGSVFFQRQSGELWGDGLTSAGVGNGSFSLQEGFEIGPTVGYFTSP
                     GLFVGGQFAILYYEQGKSNLAAIALGPTVGYFFRTGQGRTEVKGSAYPYIRGFLSWGN
                     LVSGGDVAIWQFGGKGGLLYMLSGAVALDIAARFQGDSWRPDGGLSINGTTVSVGIGV
                     SAFIY"
     gene            808..>1075
                     /locus_tag="PK112_09295"
     CDS             808..>1075
                     /locus_tag="PK112_09295"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HPC12275.1"
                     /translation="MNHFPIPAITRTSASAEELAVYRALKPYLARCLRLNHDVNNALA
                     GIIGYTDFLLEGGSVLTEDQRRFLSQIMQAAEKVRRLVEELCDEK"
BASE COUNT          191 a          319 c          343 g          222 t
ORIGIN      
        1 actatgttcc cggcgtcgcc gttcggtcgt caccgacacc gggctaccgg tcacaaagcc
       61 ttatcggagg gagtatgttc aggctgttga tttgcagtgg agcggtgctc gccatcgccg
      121 gagcagtcgc ggctgcggaa agcccgattg atgagggcag cctttacctc gatgggagcg
      181 tgttcttcca gagacaaagc ggcgaactgt ggggggacgg tcttaccagc gcgggggtcg
      241 gcaacggcag tttctcgctg caagagggtt ttgaaatcgg accgacggtc ggctatttta
      301 cgtcgccggg cttattcgtc ggcggtcagt tcgcgatcct gtactatgaa cagggtaagt
      361 ccaacctcgc ggcaattgcc ctcgggccga ccgtcggcta tttcttcaga accggccagg
      421 gccgaaccga ggtgaaggga agcgcgtacc cgtatatccg cggcttcttg agctggggaa
      481 acctcgttag cggcggtgac gttgcgattt ggcagtttgg aggcaagggc ggacttctgt
      541 acatgctgtc cggggcggtc gccctcgaca ttgccgcacg ctttcaggga gacagttgga
      601 ggccggacgg aggactctca ataaacggga ccaccgtctc ggtcggcatc ggggtttccg
      661 ccttcattta ctgacctcca accggtctgc ctggtttatg cgatgtttat gccgcgagga
      721 tgcagggcgc ggtcgcccga cacttttgtc cgtcgaccgc cccggtgatc ctcaaccccg
      781 tccccggttc tgccgattaa tccgtacatg aaccacttcc cgattcccgc cataacccgc
      841 acgagcgcct cggccgagga gctcgcggtc taccgggcgc tcaagcccta cctggcgcgc
      901 tgtctgcgcc tcaatcacga tgtcaacaac gcgctggccg gcatcatcgg ctacacggat
      961 ttcctcctcg aagggggcag cgtactcacc gaggaccagc ggcggtttct atcgcagatc
     1021 atgcaggcgg ccgagaaggt gcggcgcctg gtcgaggagt tgtgcgacga gaaag
//
LOCUS       DAOOKS010000648         1072 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_187952_length_1072_cov_4.055064, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000648 DAOOKS010000000
VERSION     DAOOKS010000648.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_187952_length_1072_cov_4.055064"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            533..>1072
                     /locus_tag="PK112_09300"
     CDS             533..>1072
                     /locus_tag="PK112_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12276.1"
                     /translation="MHPRRSGGDIRQLLTISVTGRLLTVGMGLTLLSLASMAQGGGGR
                     LHRFEVPVTAFRLQVEPVAQVVPTGVPARLRTWIELADPAAGYAESVRQYLASRYEVR
                     AELYAPAATPGYPRSLACRVGGEFLLDNLAAPGRYHLRNIRLADPDTGRDFLYAAPDT
                     AAVDVGGDLFIAWVTAEPMT"
BASE COUNT          203 a          353 c          314 g          202 t
ORIGIN      
        1 tcgctgccgc tcgcggtacc tcccgcctgc cggctgcttg atcgtgctca tggctccagt
       61 cacagaatcc tgccaaaaga tggcagaaca tcgggagtaa ctgaccaatc acccaatgaa
      121 aaatgaccaa tggaagatga ttcgcctcga ctccaaatag ccaaaaacaa ataagcaatt
      181 accgataacc aataatccga aatacccaat gacaaatgtc taatgaaaga tgatcaatga
      241 atgccgcccc ccccattcac cctttccgat cgatcgcacc gccgacgatt tgtttcgtac
      301 acccgccgcc cccatccgtt atatatattg aatggccgag atgataattt cacgaattcg
      361 acatttgttc ttgcgcgggc ggagacggtt gagtagaatt ccgacaattt acctcggatt
      421 gggagtctga gccatagggt gggctgggcc ggatgggtac cggccggccg gggagggtag
      481 ggtgttcggg aggaccggcg cgagccggag ccgctttgtt cagcggatgc ctttgcatcc
      541 gcgccgaagc ggcggtgaca tcagacaact tctgacgatc tccgtcacgg gacgtctgct
      601 gaccgtgggc atgggcctca ccctgttgtc gctcgcctcc atggcccagg gcggcggcgg
      661 caggctgcat cgttttgaag ttccggtcac cgcgttccgc ctgcaggtgg agccggtggc
      721 ccaggtcgtg ccgaccggcg tgcccgcccg cctccggacc tggatcgagc tggccgatcc
      781 ggccgccggc tacgccgaat ctgttcggca atacctggcg tcccgctacg aggtgcgcgc
      841 ggagctctac gctccggccg cgacacctgg ctacccgcgg tcgctggcgt gtcgcgtggg
      901 tggcgagttc ctcctcgaca acctggccgc acccggccgc taccacctgc gcaacatccg
      961 gctggccgac ccggacaccg gccgcgactt cctgtacgcc gcgcccgaca ccgccgccgt
     1021 cgacgtgggc ggcgacctgt tcatcgcctg ggtcaccgcc gagcccatga cg
//
LOCUS       DAOOKS010000649         1072 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_188015_length_1072_cov_2.799410, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000649 DAOOKS010000000
VERSION     DAOOKS010000649.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_188015_length_1072_cov_2.799410"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1072)
                     /locus_tag="PK112_09305"
     CDS             complement(<1..>1072)
                     /locus_tag="PK112_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12277.1"
                     /translation="RDTVVSLGADGWSSTREMFPFRRPLLRRSIVDPEDSEYEGAVSE
                     EDFVATYTDTFTAGIPPDLYGQPHIPIKIAVTENSYGWSYSYAEDFILFDYQITSMNS
                     QMLNDVYLGIYVDADVYHPTTTNGYSDDLTGYLEKYSQVIGPCSYADTINIAWLADND
                     GDLDFPRPVPHVTGMRIVRTPADTLEISFNWWFSASGELDFGPRERPYTGKWKEPFRD
                     FGTGGTGTPEGDRNKYYIMSNQELDYDQARVASIGPTDPLWVYPKQELVAEWADGADT
                     RYLLSFGPFNIRPGQTLPLSFAYVGGKNFHTYRQNLSNLPYNFDAFYDSLHFEDFAEN
                     ARWAGWIYDNPGVDTDSNGFAGR"
BASE COUNT          185 a          314 c          365 g          208 t
ORIGIN      
        1 cccggccggc gaagccgttg ctgtcggtgt cgacgcccgg gttgtcgtag atccaccccg
       61 cccagcgggc gttttcggcg aaatcctcga agtgcaggct gtcgtagaaa gcgtcaaagt
      121 tgtagggcag gttgctcagg ttctggcggt aggtgtggaa attcttgccg cctacgtaag
      181 cgaacgagag cgggagggtc tgccccgggc ggatgttgaa gggcccgaac gagagcagat
      241 agcgcgtgtc ggcgccgtcg gcccactccg ccaccagctc ctgtttcgga tagacccaca
      301 ggggatcggt ggggccgatc gacgccaccc gcgcctggtc gtagtcgagc tcctggttgc
      361 tcatgatgta gtacttgttg cggtccccct ccggcgtccc ggtgccgccc gtgccgaaat
      421 cccggaacgg ctctttccac tttccggtat aggggcgctc gcgcggcccg aagtcgagtt
      481 cgccgctggc cgagaaccac cagttgaagg aaatctccag cgtgtcggcg ggcgtccgca
      541 cgatgcgcat cccggtcacg tggggaaccg ggcgcgggaa atccaaatcc ccgtcgttgt
      601 ccgccagcca ggcgatattg atggtgtccg cgtacgagca ggggccgatg acctgcgagt
      661 acttctccaa atacccggtg aggtcgtcgc tgtacccgtt ggtggtggtc gggtggtaca
      721 cgtcggcatc gacgtagatc cccaggtaca cgtcgttgag catctgcgaa ttcatgcttg
      781 tgatctgata gtcgaaaagg atgaaatcct cggcgtagga gtaggaccac ccgtaggagt
      841 tctcggtcac cgcgatcttg atggggatgt gcggctggcc gtacaggtcg ggcgggatgc
      901 cggccgtaaa cgtgtcggtg taagtggcga cgaagtcctc ttccgaaacc gcgccctcgt
      961 attcgctgtc ttcgggatcc acaatggagc ggcggagcag gggtcggcgg aaggggaaca
     1021 tttcgcgtgt cgacgaccac ccgtcggcgc ccagcgagac gaccgtgtcc cg
//
LOCUS       DAOOKS010000650         1061 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_191136_length_1061_cov_3.314115, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000650 DAOOKS010000000
VERSION     DAOOKS010000650.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_191136_length_1061_cov_3.314115"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            328..>1061
                     /locus_tag="PK112_09310"
     CDS             328..>1061
                     /locus_tag="PK112_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HPC12278.1"
                     /translation="MGAFRLVSEFQPRGDQPQAISGLVKGLENGLGHQVLLGVTGSGK
                     TFTMANVIAAVNRPTLVIAHNKTLAAQLYGEFKGLFPDNAVEYFVSYYDYYQPEAYLP
                     STDTYIEKDSSINEEIDKLRHAATYALLERRDVIIVASVSCIYGLGSPEAYQGMLLLL
                     EEGMEIPRDAMLARLVEIQYERNDIDFHRGTFRVRGDVVEIFPAYEQERAIRVEFFGD
                     AVESISLVDPLRGRKIERIRRMPVYPG"
BASE COUNT          249 a          288 c          306 g          218 t
ORIGIN      
        1 aaaatggcgc aaaaaggcgg cggatacgtg actttctact ggccgcaccc ggaaaccaaa
       61 aatgagcaga gaaaaattgg atatgccgag cttataccgg atacggatta ttacatcgga
      121 accgggtatt acccggatac gaaataacga cttctttcat agccggcacg tccggagatt
      181 atttcatctg tctacataag actctgtgta ttctgtacat ataatttgtc aattaaacgg
      241 aaaaatcaac tcagtgaaaa ccctgagctc cagccctctt gtcatccctc aggaattgtg
      301 aaagagtcct ttcccgggag gagggtaatg ggagcgttcc gtctggtcag cgagtttcag
      361 ccgcgggggg atcagccgca ggccatctcc ggcctcgtca aggggttgga gaacggcttg
      421 gggcaccagg tgctgctcgg agtgaccggt tccggaaaga ccttcacgat ggcgaacgta
      481 atcgcggcgg tcaaccggcc gacactggtg atcgcccaca acaagaccct tgccgcccag
      541 ctctacgggg aattcaaggg actgttcccg gacaacgccg tcgaatattt tgtcagttac
      601 tacgattatt atcagcccga ggcctacctt cccagcaccg acacctacat cgagaaggac
      661 tcctcgatca acgaggaaat cgacaagctc cgccacgcgg ccacgtatgc actgctggag
      721 aggcgggacg tgatcatcgt ggccagcgtt tcctgcatct acggtctggg ttccccggag
      781 gcctaccagg ggatgctgct gctgctcgaa gaggggatgg agatcccgcg ggatgcaatg
      841 ctggcgcgtc tcgtcgagat ccagtacgag cggaacgaca tcgatttcca tcgcggaaca
      901 ttccgcgtcc ggggcgacgt cgtcgagatt ttccccgctt atgagcagga gagggcgatc
      961 cgcgtggagt tcttcggaga tgcggtcgag tcgatctcgc tggtcgatcc gctgcgcggg
     1021 cgaaaaatcg aaaggatccg gagaatgccc gtctatcccg g
//
LOCUS       DAOOKS010000651         1061 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_191227_length_1061_cov_2.339960, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000651 DAOOKS010000000
VERSION     DAOOKS010000651.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_191227_length_1061_cov_2.339960"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1061
                     /locus_tag="PK112_09315"
     CDS             <1..>1061
                     /locus_tag="PK112_09315"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005034436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HPC12279.1"
                     /translation="DKSKQIMERARRVIPGGVNSPVRAFKSVGGVPRVIARGEGAYIW
                     DADDNRYLDFGGSWGPLILGHADPEAVAAVVEQVRRGMTYGTATEIEFRLADFIVRHI
                     PAIEKIRFVSSGTEAVMSAIRVARGFTKRDLILKFDGCYHGHADPLLVQAGSGLATFG
                     TPSSAGVPEAVASLTAVLPLDDEDALERFFRQNGDRLAAAVIEGIPANNGLLIQRHEY
                     MRLLRALTEKHGALLVLDEVITGFRLGLGGALQYYGIHPDLVTFGKVIGGGMPVGAFG
                     GRAEIMDLLSPDGPVYQAGTLSGNPVAMTAGLATLEKLADGRIYADLEARNRRFVSAI
                     TARVRGNRVNVVGVASIFW"
BASE COUNT          170 a          341 c          388 g          162 t
ORIGIN      
        1 ggacaaatca aaacagatca tggaacgggc ccggagggtg atccccggcg gagtcaattc
       61 gccggtgcgg gcgttcaagt cggtgggcgg cgtgccccgc gtcatcgcca gaggggaagg
      121 cgcgtatata tgggacgccg acgacaaccg gtacctcgat ttcggcggat cctggggacc
      181 gctcattctc ggccacgccg acccggaggc ggtcgctgcg gtagttgagc aggtccggcg
      241 ggggatgacg tacggaaccg ccaccgagat cgaattcaga ctggcggatt tcatcgtgcg
      301 gcacatcccg gcgatcgaga agatccgttt cgtctcctcg ggcaccgagg cggtcatgtc
      361 ggcgatccgg gtcgcacggg gattcaccaa gcgcgacctc atcctcaagt tcgacggctg
      421 ctaccacggg cacgcggacc cgctcctcgt gcaggcgggc agcgggctgg cgaccttcgg
      481 cactccctcc tccgccggcg tcccggaggc ggtcgcgagc ctgacggcgg tgctgccgct
      541 cgacgacgaa gacgcgctcg agcggttttt ccgccagaac ggcgatcggc tggcggcggc
      601 ggtgatcgaa gggatcccgg ccaacaatgg cctgctcatc cagcgccacg agtacatgcg
      661 cctgctccgc gcgctgacgg aaaaacacgg ggcgctgctg gtgctcgacg aagtgatcac
      721 cgggttccgc ctcggcctgg gaggcgccct gcagtactac ggcatccacc cggacctggt
      781 gaccttcggc aaggtgatcg gcggggggat gccggtcggg gcgttcggcg ggcgggccga
      841 gatcatggac ctcctgtcgc ccgacggccc ggtgtaccag gcggggacgc tctcggggaa
      901 tccggtggcg atgacggccg ggctggcgac gctggagaag ctggccgacg ggcgcatcta
      961 cgccgacctc gaggcccgca accgccgctt cgtgagcgcg atcacggcgc gcgtgcgggg
     1021 caaccgggtg aacgtggtcg gggtcgcctc gatcttctgg a
//
LOCUS       DAOOKS010000652         1058 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_192027_length_1058_cov_3.890329, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000652 DAOOKS010000000
VERSION     DAOOKS010000652.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_192027_length_1058_cov_3.890329"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="PK112_09320"
     CDS             <1..802
                     /locus_tag="PK112_09320"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HPC12280.1"
                     /translation="YRVVAPIGAGAFARVYKAVDPERGGEYIALKCLTPPADGANDGR
                     AARFLREAKILRAISHPNVVRLLGFGAEPVLYLAMEFVSGGDLKARLAAGTPWTLPDA
                     LRLTRGVLRGIGAIHAAGVVHRDMKPANVLLRENREPVVADLGMGKVIGPPALTRYSM
                     LIGTIGYMAPEQLRGRRVDRRADLYAIGCILFQMLAGRLPFTGADAVEVIRKISETAA
                     PPIAALKPDLPPALAAFVDTLLAKDPARRHGSAAEALAALDGVAAEIA"
     gene            904..>1058
                     /locus_tag="PK112_09325"
     CDS             904..>1058
                     /locus_tag="PK112_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent alcohol dehydrogenase"
                     /protein_id="HPC12281.1"
                     /translation="MQNFEFHNPVKVVFGKGSIAELAELVPRGVPVLMTYGGGSIKRN
                     GVYDEVKA"
BASE COUNT          155 a          392 c          361 g          150 t
ORIGIN      
        1 ctaccgggtg gtcgcgccca tcggcgccgg cgctttcgcg agagtgtaca aggcggtcga
       61 cccggaacgc ggcggggagt acatcgccct gaaatgcctc acaccccccg cagacggcgc
      121 gaacgacggc cgcgcggcgc gcttcctccg cgaggccaaa attctgcgcg cgatctccca
      181 cccgaacgtc gtgcggctcc tcgggttcgg cgccgagccg gtcctctatc tcgcaatgga
      241 gttcgtgtcc ggcggcgacc tgaaggcgcg cctggccgcc ggaaccccct ggacgctccc
      301 cgacgccctg cgcctgacgc ggggcgtctt gcggggcatc ggcgccatcc acgccgcggg
      361 agtcgtgcac cgggacatga agcccgccaa cgtgctcctc cgggagaacc gcgagcccgt
      421 ggtggccgac ctcggcatgg gcaaggtgat cgggccgccg gctctgacca gatactccat
      481 gctgatcggc accatcggct acatggcgcc cgagcagctc cgcggccggc gcgtcgaccg
      541 ccgcgcggat ctgtacgcga tcggctgcat tctgttccag atgctcgccg gcaggctgcc
      601 gttcaccggc gccgacgccg tcgaagtcat ccggaagatc tcggaaacgg cggcgccgcc
      661 catcgccgcg ctgaagcccg atctcccgcc cgcgctcgcg gccttcgtcg acacgctgct
      721 ggcgaaggac cccgcgcgcc gccacggcag cgccgccgag gcgctcgcgg cgctcgacgg
      781 cgtcgcggcc gagatcgcct agcgtccgtc ctcggtcagc gccttgccgg atccgcggcg
      841 cgccgctacg ataccctcga agcggcggtt gagcccgcgc ggggggcccg gaaaggacac
      901 ggcatgcaga acttcgagtt tcacaatccg gtgaaggtgg tgttcggcaa aggctccatc
      961 gccgagctcg ccgaactggt gccccgcggc gtcccggtcc tcatgactta cggcgggggc
     1021 tcgatcaagc gcaacggggt ttacgacgag gtcaaggc
//
LOCUS       DAOOKS010000653         1042 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_196840_length_1042_cov_4.264438, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000653 DAOOKS010000000
VERSION     DAOOKS010000653.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_196840_length_1042_cov_4.264438"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="PK112_09330"
     CDS             complement(<1..569)
                     /locus_tag="PK112_09330"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HPC12282.1"
                     /translation="MTAPKLLSWIELSQSALRRNIESLRRLAGARMVAPSVKSNAYGH
                     GLTEIITMLRRSGGCEYVTVHSLEEALTCRRAGWTEGIMVLGPLPPGEYGQVIEQDLE
                     PAVFDLVTLRELGKLGDRKGAAIRTHLKLETGTNRQGFAAQDLPAVAGLYKRFRSLGR
                     PHGASMHFANIEDTTVHEYAEQQLRAYRDL"
     gene            728..>1042
                     /locus_tag="PK112_09335"
     CDS             728..>1042
                     /locus_tag="PK112_09335"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF015280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HPC12283.1"
                     /translation="MLLAIDIGNTNVVFGLFRGADLVGDYRIATPPRSTPDEAGLSIT
                     HFLMRHRIEPARVEKVVIASVVPHLTPVFERTSRRYLSREPLTVSCRLKLPIAIRIES
                     PDQ"
BASE COUNT          153 a          418 c          291 g          180 t
ORIGIN      
        1 agatcgcggt aggcgcgcaa ctgctgctcc gcgtactcgt gcaccgtggt gtcttcgatg
       61 ttggcgaagt gcatcgacgc cccgtggggg cgcccgagcg agcgaaaacg tttgtacagc
      121 ccggccacgg cggggagatc ctgggctgca aacccctggc ggttcgtccc cgtctcgagc
      181 ttgaggtgcg tccggatcgc cgcccccttc cggtccccca gcttgccgag ttcccgcagc
      241 gtcaccagat cgaaaaccgc cggctccaga tcctgctcga tcacctggcc gtactccccc
      301 ggcgggagcg ggccgagcac catgatcccc tccgtccacc ccgcccggcg gcaggtgagc
      361 gcctcctcca gtgagtgcac ggtcacatat tcgcacccgc cgctgcggcg aagcatggtg
      421 atgatttcgg tcagcccgtg cccgtaggcg ttgctcttga ccgacggcgc caccatgcgc
      481 gcccccgcca gccgccgcag cgactcgatg ttccgtctca gcgcgctctg cgacagctcg
      541 atccaactca gcagtttcgg ggcggtcata gggatagttt atacagacgc cgcgccgggc
      601 gcgacaacaa aaagtttccc ctcccccgcc cgccgaccga ctcccgcggc cccgttttcc
      661 gcccgcgatg atccccccgt tctccttcgc cgttgccccc cgccggcctt tccgttatat
      721 tcgccccatg ctgctcgcca tcgacatcgg caacaccaac gtggttttcg gcctcttccg
      781 cggcgccgac ctggtcggcg actaccgcat cgccaccccg ccccgcagca cgcccgatga
      841 ggccggcctg tcgatcaccc atttcctcat gcgccaccgc atcgagcccg ccagggtgga
      901 gaaagtcgtg atcgcctcgg tcgtcccgca cctgaccccc gtgttcgagc gcaccagccg
      961 ccgctacctc agccgcgagc cgctcaccgt ctcctgccgc ctgaaactgc ccatcgcgat
     1021 ccgcatcgag tcccccgacc ag
//
LOCUS       DAOOKS010000654         1035 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_199098_length_1035_cov_3.493878, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000654 DAOOKS010000000
VERSION     DAOOKS010000654.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_199098_length_1035_cov_3.493878"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..372
                     /gene="rpsO"
                     /locus_tag="PK112_09340"
     CDS             103..372
                     /gene="rpsO"
                     /locus_tag="PK112_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HPC12284.1"
                     /translation="MPLNKEHKAKIIEENRLHGTDTGSPEVQIALLTARINHLTEHLK
                     SHSKDFHTRLGLLKLVGQRRRLLDYLMDRDIESYRQLIGELNLRR"
     gene            393..>1035
                     /locus_tag="PK112_09345"
     CDS             393..>1035
                     /locus_tag="PK112_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010898560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HPC12285.1"
                     /translation="MHERHKVEFELGGRTMIIEQGYLANQANGAVTVRYGDSMVVSTA
                     CAQSEPKVDQDFFPLTVEYRERSYAAGKIPGGFFKREGRPSEKETLASRLIDRPIRPL
                     FPKEFRGETQCITLVISHDQANDTDILALTGTGAALAVSDIPFEKTIAGVRVGRINGE
                     FVINPTISQLEESDLYITMAGSADAITMVEGGGREIPEDVMIDALLFGHDHIKL"
BASE COUNT          241 a          357 c          278 g          159 t
ORIGIN      
        1 atgtccgcga aaaccggcgg tttgagtcca gggaagcgct cgtcgcccag atcggcaaag
       61 acaaagaagt gattttagac atagtgaaga aggagaaacg gtatgccgct caacaaggag
      121 cacaaagcca aaatcattga ggaaaaccgc ctgcacggca cggacacggg ttcgcccgag
      181 gtccagatcg ccctgctgac ggcccgcatc aaccatctca cggaacacct gaagtcccac
      241 agcaaggact tccatacccg cctcgggctg ctgaaactgg tcggccaacg ccgccggctg
      301 ctcgattacc tgatggaccg cgacatcgag agctatcgcc agcttatcgg cgagctcaat
      361 ctccgccgct aggcgaaggg aaccaatgta gtatgcatga aagacacaaa gtagaattcg
      421 aactcggcgg ccgcacgatg atcatcgagc agggctacct ggccaaccag gccaacggcg
      481 ccgtcaccgt ccgctacggt gactccatgg tcgtttccac cgcatgcgcc cagtccgaac
      541 ccaaggtcga tcaggatttc ttccccctga ccgtcgagta ccgcgagcgc tcctacgccg
      601 ccggcaagat cccgggcggt ttcttcaagc gcgagggccg gccgtcggag aaagaaaccc
      661 tcgcctcccg gctcatcgac cggcccatcc ggcccctctt ccccaaagag ttccgcggcg
      721 agacgcagtg catcaccctc gtcatctccc atgaccaggc caatgacacc gacattctcg
      781 ccctcaccgg caccggggcc gcgctcgccg tctccgacat ccccttcgaa aaaaccatcg
      841 ccggcgtccg cgtcggccgc atcaacggcg aattcgtcat caacccgacc atcagccagc
      901 tcgaagaaag cgacctgtac atcacgatgg ccggcagcgc cgatgcgatc acgatggtcg
      961 agggaggagg ccgggagatc cccgaggacg tcatgatcga cgccctcctg ttcggccacg
     1021 accacatcaa gctca
//
LOCUS       DAOOKS010000655         1035 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_199105_length_1035_cov_3.298980, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000655 DAOOKS010000000
VERSION     DAOOKS010000655.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_199105_length_1035_cov_3.298980"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            414..968
                     /locus_tag="PK112_09350"
     CDS             414..968
                     /locus_tag="PK112_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12286.1"
                     /translation="MGSVRPAVLCGVVLLSAAVSAATLPSRAIVHDCPAELFAQQFSL
                     RVLDPAEAAALGGKSFSGDTLKLLVIAVEWLEPPEKRPSTYAVDTLRQLFFSRDLMPH
                     GSVADYFDEVSYGQLQVTGQVYGWFNNGSYVASGDSLLYLLAELDSLIDFTQYDGNGD
                     GRVDGVVFVVAGRNRPDTNCLTDI"
BASE COUNT          162 a          276 c          383 g          214 t
ORIGIN      
        1 gtccatctcg gcgcgcgtct cttcggtgcg ggccgcctca ccggcgccac ggagagagcg
       61 catggtgatg gcggcgagga tgccgatgat gacgatcacg aggatcatct caaagaggga
      121 gtatccgcgc tgcgaggcgc ggacgagaat gtggtgccgg gtcgtcattt tgttcgactg
      181 ctagtcaggt tccgaggggc cggcggcggc tctgccgacg gatcggcgca ctcggcctat
      241 ctctgttatc ggcaggaagt tagagcgggt ttacgggagt cggcgggcgg ggactgcggc
      301 gcgccgcaag ggcgcggagc caaaaacgcg gttgcggaat cggtaatgtg tcctatattg
      361 tcgatagcat ttatcttatt ttggttgagg cggttggccc aggtgaggtg ttgatgggtt
      421 cggtccggcc ggcggtgctc tgcggggttg tgctgttgtc ggcggcggtg tcggccgcga
      481 cgttgccgtc gcgcgcgatc gtgcacgact gcccggccga gcttttcgcg cagcagttca
      541 gtctgcgcgt cctcgatccg gccgaagcgg cggcgctggg aggaaagtcg ttctcggggg
      601 acacgctcaa gcttctcgtg atcgcggtgg agtggctgga gccgccggag aaacggccct
      661 cgacctatgc agtcgatacg ctgcggcagt tgttcttctc gcgcgacctg atgccccacg
      721 gatcggtggc cgactatttt gacgaggtgt cctacgggca gctccaggtg accgggcagg
      781 tctacggatg gttcaacaac ggttcctatg tcgcttcggg agattcgctg ctctacctgc
      841 tcgcggagct ggactcgctg atagacttca cgcagtatga cggcaacggg gacgggcggg
      901 tggacggggt ggtgtttgtg gtggcggggc ggaaccggcc ggacaccaac tgcctgacgg
      961 acatatgaac ggcggcgatc acgtttcctc cgggcgagga attcgggcct ttcgacggaa
     1021 tgtacgtgaa tcagc
//
LOCUS       DAOOKS010000656         1035 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_199156_length_1035_cov_2.655102, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000656 DAOOKS010000000
VERSION     DAOOKS010000656.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_199156_length_1035_cov_2.655102"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="PK112_09355"
     CDS             <1..199
                     /locus_tag="PK112_09355"
                     /inference="COORDINATES: protein motif:HMM:NF017200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12287.1"
                     /translation="IEEVILPEDNRKDIAEIPPEIKKAIRFKYFSAAIEAIKYALDRP
                     AVCGGAAANAAAGRKRKRPGR"
     gene            196..>1035
                     /locus_tag="PK112_09360"
     CDS             196..>1035
                     /locus_tag="PK112_09360"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HPC12288.1"
                     /translation="MKLVTAEQMRAIDRETIEVHGIPGPVLMENAGRGIAERLAVAVI
                     PKVRGAKVAVVCGKGNNGGDGFVVARHLAGMGAQVAVYYLGPPEQMSEDCRLNFERAK
                     ELGFPMTEVTAVEALPETLPDDFIVDAVFGTGFSGAPRGLAGELIEYINRHEDRTVIA
                     VDMPSGLNADTGEGEGAVVQAHYTFPLALPKYGLYVSPGRELAGAVAVVPIGVPDEVL
                     AKFDLRSDLITLGMVRQWLPDRPPDGHKGTFGRLLIVAGSLGMTGAAALAGTAAYRCG
                     CGLV"
BASE COUNT          172 a          356 c          359 g          148 t
ORIGIN      
        1 catcgaggag gtcatcctgc cggaggacaa ccgcaaagac atcgccgaga tcccgccgga
       61 gatcaagaag gcgatccggt ttaagtattt ctccgcggcg attgaagcaa tcaaatatgc
      121 gctcgaccgc cccgccgtgt gcgggggcgc ggcggccaac gccgcggccg ggcggaaacg
      181 gaaacggccg ggccgatgaa actcgtcacc gccgaacaga tgcgcgcgat cgaccgggaa
      241 acgatcgagg tccacggcat ccccggtccc gtcctcatgg agaacgccgg gcgggggatc
      301 gccgaacgcc tcgccgtcgc cgtcatcccc aaagtccgcg gcgccaaagt cgcggtcgtc
      361 tgcggaaagg ggaacaacgg cggcgacggt ttcgtggtcg cccggcacct cgccggcatg
      421 ggcgcccagg tcgccgtcta ctacctgggc ccgcccgagc agatgtcgga ggactgccgc
      481 ctcaacttcg aacgcgccaa agagctcgga ttcccgatga ccgaggtgac ggcggtcgag
      541 gcgctcccgg agacgctgcc cgatgacttc attgtcgacg ccgttttcgg caccgggttc
      601 tccggggcgc cgcgcggtct ggcgggagag ctgatcgagt acatcaaccg ccacgaggac
      661 cggaccgtca tcgctgtcga catgccctcg ggcctcaacg ccgacacggg cgagggggag
      721 ggggcggtcg tacaggccca ctacactttc ccgctggccc ttcccaagta cgggctctac
      781 gtgtcgcccg ggcgcgaact ggccggcgcg gtggcggtgg tgccgatcgg ggtgcccgat
      841 gaagtgctgg cgaaattcga cctccgctcc gacctgatca ctctcggcat ggtgcgccaa
      901 tggctgcccg accgcccgcc cgacgggcac aaggggacat tcggccggct cctgatcgtg
      961 gccggctcgc tcggcatgac cggcgcggcg gccctggccg ggacggccgc ctaccgctgc
     1021 ggctgcgggc tggtc
//
LOCUS       DAOOKS010000657         1032 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_200100_length_1032_cov_2.921187, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000657 DAOOKS010000000
VERSION     DAOOKS010000657.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_200100_length_1032_cov_2.921187"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..931
                     /gene="truA"
                     /locus_tag="PK112_09365"
     CDS             74..931
                     /gene="truA"
                     /locus_tag="PK112_09365"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HPC12289.1"
                     /translation="MAERNIRLTLEYKGTAYAGWQIQAGQPTVQGELVAAIRKTTGCD
                     VSVHGAGRTDAGVHALGQVANFRIDHRLAPDRYRDALNYYLPADIRIREAAEAPLSFH
                     ARRSAKAKRYRYVIAGERSALWREYRWELAGREAAGASPEAAGVLPPADSLAGPGPGD
                     SPAAPAPALRFDLLQQAAALIVGEHDFAPFCVTASRKEDNRCRIDRAHWYRVGPLRVF
                     EIRGNRFLHNMVRSLVGAMVNLAAESPDRHPDNLTLTRFADIIQAPNQQRIVFTAPAR
                     GLYLVSVHY"
     gene            944..>1032
                     /locus_tag="PK112_09370"
     CDS             944..>1032
                     /locus_tag="PK112_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012371594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HPC12290.1"
                     /translation="MKFFIDTANIAQIKKAHAMGVLDGVTTNPS"
BASE COUNT          172 a          347 c          358 g          155 t
ORIGIN      
        1 gcgggcggca ggaagcgggg cgcgacccgg agcccgccgc ctcgagccgg ttctgtgcgg
       61 cgacggatag cgaatggctg aacgcaacat cagactcacg ctcgagtaca aggggaccgc
      121 gtacgcgggg tggcagatcc aggcggggca gccgaccgtg cagggggaac tagttgccgc
      181 aatccgcaag acaacgggct gcgacgtgag cgttcacggc gcggggcgga ccgatgccgg
      241 cgtgcacgcg ctcggccagg tcgccaattt ccgcatcgac caccgcctgg cgccggaccg
      301 ctacagggac gccctcaatt actacctccc cgcggatatc cgcatccggg aggcggccga
      361 ggcgcccctg tcgttccacg cccgccggag cgccaaggcc aagcggtacc gctacgtgat
      421 cgcgggggaa cgctcggcgc tgtggcggga gtaccggtgg gaactcgcgg ggcgagaggc
      481 ggccggggcc tctcctgaag cggcgggcgt cttaccgccg gccgatagtc ttgccggccc
      541 cgggccgggg gacagcccgg ccgctccggc gcccgcgttg cgtttcgatc tcctgcagca
      601 ggcggcggcg ctcattgtcg gcgagcacga tttcgccccg ttctgcgtga ccgcctcccg
      661 caaggaggat aaccggtgcc ggatcgaccg ggcgcactgg taccgggtcg gcccgctgcg
      721 ggtgtttgag attcgcggga accggttcct gcacaatatg gtacggagtc ttgtgggggc
      781 catggtgaac ctcgccgccg agtcccccga ccgccacccg gacaacttga ctttgacccg
      841 gtttgccgat atcatacaag ctccgaacca gcagcgcatc gtgttcacgg cgccggcccg
      901 ggggctgtac ctcgtgtcgg tacactactg aagggatgat cagatgaagt ttttcatcga
      961 caccgccaat atcgcgcaga tcaagaaggc gcacgccatg ggcgtgctcg acggcgtcac
     1021 caccaacccc tc
//
LOCUS       DAOOKS010000658         1031 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_200465_length_1031_cov_2.709016, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000658 DAOOKS010000000
VERSION     DAOOKS010000658.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_200465_length_1031_cov_2.709016"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1031
                     /locus_tag="PK112_09375"
     CDS             <1..>1031
                     /locus_tag="PK112_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC12291.1"
                     /translation="TRLSDGTAICLYNCEDTTGADAAGAEWDIHWYTGDGVPDFRGAA
                     PPPAPKFQLIPSFGKVRVRFNGTYSETTEDPFVGLVDFEGYRIYIGRDDRKTSFSVVC
                     GYDRENYNSFHWLPDRRTWEISPVPLTREELACRFGDSCRDANFDPLAYSMVEPLRTP
                     AGELYYFTPQDFNRSVLGIPGAIRRAYPDQPKPATDDPRQADPDELAPDGLFKYYEYE
                     YDIEGLLPSVPYWINVTALDYGSPRSGLPSLESAVTQGAQYVIPLSDPEKARSEELPI
                     IVYPNPYRIDAEYRSLGFEGRGQDDRPDHRVRCVRFANLPPRCTIRIFSLDGDLIRTL
                     EHDMPPSDP"
BASE COUNT          177 a          367 c          313 g          174 t
ORIGIN      
        1 gacgcggctg tcggacggca cggccatctg cctttacaac tgcgaggaca cgaccggcgc
       61 ggacgcggcc ggcgcggaat gggacatcca ctggtacacg ggggacggag tgccggactt
      121 ccggggcgcg gccccgccgc cggctcccaa gttccagctc ataccttcgt tcgggaaggt
      181 tcgcgtccgc ttcaacggca cctactccga gacgaccgaa gatccgttcg tgggcctggt
      241 ggatttcgag ggttaccgca tctacatcgg acgggacgac cgcaagacca gtttcagcgt
      301 cgtgtgcggg tacgatcggg agaactacaa cagcttccac tggctgcccg accgccgcac
      361 ctgggagatt tcgccggttc cgctcacgcg cgaggaactc gcctgccggt tcggcgactc
      421 gtgccgcgac gccaacttcg accccctcgc ctacagcatg gtggagccgc tgcggacacc
      481 cgccggcgag ctgtactatt tcacaccgca ggatttcaac cgctcggtgc tgggaatccc
      541 cggcgccatt cgccgcgcct atcccgacca gcccaagccg gcgactgacg acccgcgcca
      601 ggcggacccc gacgaattgg cccccgatgg tcttttcaag tactacgaat acgagtacga
      661 catcgagggg ctcctcccct cggtgccgta ctggatcaat gtcaccgcgc tcgattacgg
      721 atctccccgg tccggcctgc cgtcgctcga atcggccgtc acccagggag cccagtacgt
      781 gatcccgctg tccgatcctg agaaggcgcg ctcggaggag ctcccgatca tcgtctatcc
      841 caacccgtac cgcatcgacg ccgagtatcg ctcgctcgga ttcgagggcc gggggcagga
      901 tgaccggccg gaccaccgcg tgcggtgcgt gcgttttgca aatctgccgc cccgctgcac
      961 gatccggata ttctctctcg acggagacct gatccggacg ctcgagcacg acatgccgcc
     1021 gtcggacccc a
//
LOCUS       DAOOKS010000659         1028 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_201405_length_1028_cov_2.823227, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000659 DAOOKS010000000
VERSION     DAOOKS010000659.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_201405_length_1028_cov_2.823227"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="PK112_09380"
     CDS             <1..205
                     /locus_tag="PK112_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010639776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HPC12292.1"
                     /translation="ESVQWAHTLGEVVTALAGAGLAIDRLEEFDGFCYPYREGWVERD
                     GYWYPPEGPPPYPMLFALLARKP"
     gene            224..>1028
                     /locus_tag="PK112_09385"
     CDS             224..>1028
                     /locus_tag="PK112_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HPC12293.1"
                     /translation="MEPTAITAALRRTELFREIDVDELEALVAITVARPLAAGEGLFR
                     QNTPRQNLWIIAEGTVELYMTTAWGGEKRLARFGPGDFLGEGALMDDYPHSTSARAVT
                     PGTVLVVSRANFAALVTRQPEIAVKVLSRVARVISRRMRQASTRVVNAGAQYVSGRTR
                     AEHDLLGPREVPHEFYYGIQTLRALENFNISGISLMHFPVLIESLAMVKMAATQANHD
                     LGLLPDDVTDAINQACREIIDGRWHTQFVVDMIQGGAGTSTNMNANEVIA"
BASE COUNT          175 a          347 c          342 g          164 t
ORIGIN      
        1 agagtcggtg cagtgggcgc acacgctcgg ggaggtggtg acagcgctcg ccggggcggg
       61 gctggcgatc gaccggcttg aagaattcga tggattttgc tacccttacc gggaggggtg
      121 ggtcgagcgc gacggctact ggtatccgcc ggagggcccg cccccgtacc cgatgctctt
      181 cgcgctcctc gcccgcaaac cctaagagaa tgaggtccga catatggaac cgaccgccat
      241 caccgccgcc ctgcgccgca ccgagctgtt tcgcgagatc gacgtcgatg agctcgaggc
      301 gctggtggcc atcaccgtcg cccggccgct cgcggccggg gaaggacttt ttcggcagaa
      361 cacgccccgg cagaacctct ggatcatcgc cgagggaacg gtggaactgt acatgacgac
      421 cgcctggggc ggggagaagc ggctggcgcg cttcggcccg ggggatttcc tcggcgaggg
      481 ggcgctcatg gacgattacc cgcactcgac atcggcccgc gccgtgactc ccggcaccgt
      541 cctcgtcgtc agccgggcga acttcgccgc cctcgtgacc cggcagccgg agattgcggt
      601 gaaagtgctt tcgcgggtgg cgcgggtgat ctcccgccgt atgcggcagg cgagcacgcg
      661 ggtggtcaat gccggcgccc agtatgtctc cggccgcacc cgggcggaac atgatttgct
      721 cgggccgcgc gaggtgccgc acgagttcta ctacggcatc cagaccctgc gggcgctcga
      781 aaatttcaac atcagcggca tctccctcat gcactttccg gtgctgatcg agtcgctcgc
      841 catggtgaaa atggcggcga cccaggccaa ccatgatctc ggcctcctgc cggacgacgt
      901 caccgacgcc atcaaccagg cctgccgcga gatcatcgac ggccgctggc acacccagtt
      961 cgtcgtcgac atgatccagg gcggcgccgg cacctcgacc aacatgaacg ccaacgaggt
     1021 gatcgcca
//
LOCUS       DAOOKS010000660         1024 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate
            AMR_MDS_2721 NODE_202845_length_1024_cov_1.768834, whole genome
            shotgun sequence.
ACCESSION   DAOOKS010000660 DAOOKS010000000
VERSION     DAOOKS010000660.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864796
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:20:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,878
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,832
            CDSs (with protein)               :: 1,832
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 7 of 25
            Pseudo Genes (incomplete)         :: 15 of 25
            Pseudo Genes (internal stop)      :: 4 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_202845_length_1024_cov_1.768834"
                     /isolate="AMR_MDS_2721"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="PK112_09390"
     CDS             <1..524
                     /locus_tag="PK112_09390"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HPC12294.1"
                     /translation="LINSGMPGLPTPEPAGDADSQTDAPSLVGMLDMLLGEGNLLGRA
                     LTAPGGAFADDQVWNEERVRSAQVPAANGVTNAASLARMYAALVSEVDGVRLLDADTL
                     ERAIAPQVSGPSTVPLLDIPFALGFMTHSPLSPLLDGRSFGHYGAGGQTHPEAQGSHD
                     SHSHSPSCQKVSR"
BASE COUNT          158 a          353 c          330 g          183 t
ORIGIN      
        1 cgctcatcaa ctccggcatg cccggtctgc ccacccccga acccgcgggc gatgccgaca
       61 gccagaccga cgcgccgtcg ctggtcggca tgctcgacat gctgctcggc gaggggaacc
      121 tgctcggccg ggcgttgacc gctcccggcg gcgcgttcgc cgacgaccag gtctggaacg
      181 aggagcgggt ccgatcggcg caggtccccg ccgccaacgg cgtcaccaac gcggcgtcgc
      241 tggcccgcat gtacgcggcg ctggtgtcgg aggtcgacgg cgtccgcctg ctcgacgccg
      301 acacgctgga gcgggcgatc gccccccagg tcagcggacc cagcacggtg ccgctgctcg
      361 acatcccgtt cgctctcggg ttcatgaccc actcgccgct gtcgccgctg ctcgacggac
      421 ggtccttcgg ccactacggc gccggcggcc aaacccatcc agaggcacaa ggctcgcatg
      481 attcccatag tcattctcct tcctgtcaaa aagtatctcg atgaattatc ggcagaagga
      541 cgccaacggt tgacgggaat tggcgccaat cctcgttttt cggacgcgcc taatctacac
      601 tttcggccgc ggcgtgtaaa gagggaggcg ccggcagcgg cggcgggtcg ctgagttttg
      661 tgtaatgggg cgggcgttct tacgcttggc ggagggccgg gctactgggc cgtcttcgcc
      721 ttcgccttca gctcgcggac cttgcgcttc aggcgctcgc ggtttttgcg gtgcttcttg
      781 acggcgagtt tcttcttctt gtacatggtg cgttcactcc acaagccttc tcggccgaac
      841 cgctcgacgc ggcacctcga ccgcacccga gcgacggcgc aacaacccat ggtactactc
      901 ttcaggctcc gagggaagtt cgccgagcgg catgacctcg ggaaaatccc ggctgcggta
      961 cgggcggatc gcgtctccct cgcggtggag aaacaaagcg tgcacgcccg ggaggcgctc
     1021 cagg
//
